LOCUS       DACEOM010000001        72906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEOM010000001 DACEOM010000000
VERSION     DACEOM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.001"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..206)
                     /locus_tag="IW781_000001"
     CDS             complement(24..206)
                     /locus_tag="IW781_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181028.1"
                     /translation="MYVIVDGIVGPWFLEPWKALAQEDYEVHYIVLRASMEIRLRSKL
                     KSVLIVQYLKILYNRN"
     gene            complement(197..463)
                     /locus_tag="IW781_000002"
     CDS             complement(197..463)
                     /locus_tag="IW781_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181029.1"
                     /translation="MSVPEVPRANRKATGRCVQPANIKKIHDIIRCALNQAIRWEYID
                     TNKRNPASLATLPKIPKTRRKVWSVQTFREAVKATEDDLLSICM"
     gene            complement(487..918)
                     /locus_tag="IW781_000003"
     CDS             complement(487..918)
                     /locus_tag="IW781_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181030.1"
                     /translation="MDEQLTFEFEQRPTIKGFPELRWTGKRPYRSTQYYPAQLRESYG
                     EEQNGWINKIFWGDNLQVMSHLLKDYRGKIDLIYIDPPFDSKADYKKKIDLKGIGKAT
                     SDSSSFEEKQYGDIWTNDEYLQFMYERLLIMRELLSEKGSD"
     gene            complement(924..1682)
                     /locus_tag="IW781_000004"
     CDS             complement(924..1682)
                     /locus_tag="IW781_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181031.1"
                     /translation="MPDIYDPERGLKIGLTDDDYDDYQKGNLSDDFYARRTDGSIANK
                     VDISRLDDSDEYEEPSNGIDAGSAAIGGLIVAAIIGAGYGIKKLWDRHKVKKTEAQVA
                     KQRKAQVNLQEEENIQMAIEPTDTVVSTDAVQSNDAERRSLTQEEAIQELMKIVVGMA
                     EIADGRKKVSEGIENLSNAGIVDRATLLEKLSVPNVLEGFNAYLENNPQIVMQNQTIF
                     GSIFGRNLTANGQYIPLDMSDIEHQLRTETIQED"
     gene            complement(1685..4570)
                     /locus_tag="IW781_000005"
     CDS             complement(1685..4570)
                     /locus_tag="IW781_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1181032.1"
                     /translation="MFNFGNANEGQRKAIAAAEGPVLITAGPGTGKTYTLVQRAIYLI
                     EECGVKPESIFIATFTEKAAKELITRITNELSDRGIVANINEMYIGTFHSLCLRILKE
                     HLEFTRLRRNYRLLDAFDQQYMVFQNIHRFRNIQEVEVALPNGGAWKQSEAICNYVNN
                     LSEELVTPEELMSDTDLSIAALGRMLKEYRDVLTEGNLMDFSSIQIEAYHLLRDNAGI
                     LEELRSKVTHIMVDEYQDTNFIQEQLVFLLAGDRKNICVVGDDDQGLYRFRGATIRNI
                     LEFPQKFADGECRVIPLVINYRSNSDIVDFYNEWMATTDGAKFKFRWDNFRYDKRIEP
                     HEKTTLHSPAVVKLAGVDDTDEWHEKILQFINNLKASGKLTDYNQIAFLFNSVKHPRV
                     TALARFLEENHINVYSPRSDMFFQRDEIRLALGCLMLMFPRYIQGLENGDYTFLQPAH
                     LTYYRNCIMAANEYLTQPENSNLLKWIRRRGKDHVGLKGATDYAYCGLMYQLFEFQPF
                     AGILDTEMDVGVVDIRPTRNLAKLTQIIGKFEYLHRVDVLDAGEYRGKRRIDQNTELL
                     FNMYLRLLFDGGITEYEDDSEYAPSGCVSFLTIHQSKGMEFPIVLVDSLANVPRKNTN
                     DLMMQIEERYFKRPAFEPYDVTKYFDFWRLYYTAFSRAQDLLILTCCEDKRTPSNYFK
                     DIYGELQSVESPGFDIREFDFKPIKGVNIKNTYSFTSHITVYETCALQYKFYKELEFM
                     PVRANAMLFGTLVHETIEDIHRAALRHEEQTITEENVSRWFDSNYVSLTKTEHTYLAG
                     PQREAALKQVLRYVERQHGDWSQIQQAEVDVSLVKPDYIIEGKVDLIRGEGDTVEIVD
                     FKSQRKPDMVKDREQLERYRRQLHIYAHLVEERTGQKVSKMHIYYTGEDSGVPTITYI
                     YTKSAVEGTMAVFDDTVKKIMKKDFRRCAEDPKICNACDFRFYCRRK"
     gene            complement(4598..4822)
                     /locus_tag="IW781_000006"
     CDS             complement(4598..4822)
                     /locus_tag="IW781_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1181033.1"
                     /translation="MVISYKKLWKLLIDKDMKKRDLREAAQLSSSTVAKLTHGENVST
                     AVLLKICAALQCDISDIMEVVPDGSPASED"
     gene            5113..6273
                     /locus_tag="IW781_000007"
     CDS             5113..6273
                     /locus_tag="IW781_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1181034.1"
                     /translation="MKAELIANIIAAHCSGDESSFKTAVEALAADEDKKGNSRVSSML
                     LDAYKGKNITLMKKPEVGQPAGGGFAVQSAGGSSFTPRDKDSLLELYDIVHSDISLSD
                     VVLPENQRKLLLQILEERQNNSKLAKHNLPPANRLLLCGPPGCGKTMTANAIAHELNL
                     PMAYVRIDGLISSYLGQTSVNLRKIFSSVGNQNIVLFLDEFDAIAKKRDDGHEMGELK
                     RVVTALLQNFDNMPPNVFLIAATNHEHLLDPAIFRRFNYTINIGYPDAKQRSELIQRW
                     FKDYTVETKVDIQKLTDLTADRSVAQIKELVISAAKRYVTTDKAVTMEDMIELLIQQM
                     TNNSNGAGVVDAAADLKAKGVSVRTLAKAIGMPHNTLSYQINKITKGKEVDE"
     gene            6276..8552
                     /locus_tag="IW781_000008"
     CDS             6276..8552
                     /locus_tag="IW781_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HAQ1181035.1"
                     /translation="MADDFRPHLLITEDDVEAIENKKQARSKDLGLDRMKHGTKLSTG
                     LQEIVSAYTRVQGTDSLRDEDIRLFEVVLQEGEKFSNKTLREFLEQEGMTFAKVKDSR
                     HAIVSSSKSKFDSLQQRVGAYRDGKRSNKKFQYIDDFQFPDGMGKQAPSIKELLEREA
                     TFPLDVEIMEQLLPKGTDPQVQVRAEERLIALIEQNQGKIQAKPYKLSDGTPIVRAEI
                     PLGKLEEISRDTIVSHVAPTGFYATSPMYTVPAQAQMSLNPNVSIDELPIVAVLDTGV
                     DFPAELEPLIVEHWVPTGAAPGDKKHGTNVASKVAFENLGEQLASGVLTPRARIIDCN
                     IRGLDPDSHKPDRPDLICNSTMIARIKEAVLRYKDITKIFNFSSSEETPIQGDEISIL
                     GYELDVLAIQYGVKFTISAGNHYLYRSQDSLEDILQDDDNRIAAPADSMLNIAVGAIV
                     GAEHKEGLSRQYDVAPYSRIGPGFRGFRKPDIVSLAGTMTKADFVPPDEYAMMIASGG
                     QWAFEAGTSFTAPTVAGDLAEISQSVPNQDILMSEALLYHGAEMPISEVAKKKITRDE
                     NAFYGNLYGRGIANPLVSMFSTAHRVTFLHRGTMNKKFKQHAKFLMPSICDTQMDMSK
                     RDKKIRVTVTCVTQPPVDKTKGADYLGAYVNASLHSRNSKDNLVTNNPSESDGRKDWD
                     TCFHFEQEFSSFHGGDWEVWLELHTRYDVEDDQEVDYALAITIEDLTKSLQLYDAVVN
                     EAQNRFPAVQLVRLPVRT"
     gene            complement(8604..9008)
                     /locus_tag="IW781_000009"
     CDS             complement(8604..9008)
                     /locus_tag="IW781_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1181036.1"
                     /translation="MLLLGYAYGIIKEVQARRDYIMNELGTYIKEKRTEKGLSIRRLA
                     ELAAISHTEVKRIEDGLRKQTSPQVLRSIASALGVPYEDLMAAAGYIDEPTAETESST
                     VATGIKDTEDLSQEEIDQVNQYIAFLKSRRKD"
     gene            9117..9356
                     /locus_tag="IW781_000010"
     CDS             9117..9356
                     /locus_tag="IW781_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1181037.1"
                     /translation="MVESLGFGKEYQMKPNIEYVKQEMERRKWTGSQLAMKMGVSRME
                     VSRLLRGRRVGGKKCIGGLIKAFPDAELKDLFFLD"
     gene            9864..10376
                     /locus_tag="IW781_000011"
     CDS             9864..10376
                     /locus_tag="IW781_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181038.1"
                     /translation="MKNNANQTTSTIYYRPLKQWIDVTPEQKRDWERFVGTTRKAKQR
                     AGACCIPYKKSYKCDGICDTCEFRCIPKDAPQHLSIDTEMENAYENGVSRTSFLADSR
                     LTTEIDIDSLILNGLLTELQSSDPESYEILMAIADGLSERAGAERLQMPRNTFVYKRN
                     QLLKRLKEKF"
     gene            10611..10928
                     /locus_tag="IW781_000012"
     CDS             10611..10928
                     /locus_tag="IW781_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HAQ1181039.1"
                     /translation="MSRMSDMAQTIEDLRSAAAAISDAADWLTKVFSSEPQAEDAPAS
                     PPEPALTLEQVRAVLADKSRQGHTAEIRALLQKYGASKLSQIDPAHYKALLAEAEVLT
                     NGS"
     gene            10918..12045
                     /locus_tag="IW781_000013"
     CDS             10918..12045
                     /locus_tag="IW781_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HAQ1181040.1"
                     /translation="MAVKHAVLSASSSERWLNCPPSARLCEAYEDKGSDYAAEGTDAH
                     ALCEFRLKQALGIPADDPIENLSWYNEEMEDCAAGYAAYVSELLETAKQTCADPVILI
                     EQRVDFSRWVQDGFGTADCIVIADGELNIVDYKHGKGVEVSAVDNPQMMLYALGALEI
                     FDGIYDIDSVRMTIYQPRKSNISVCVMEKDGLLEWAQNDLTYKAKLAYEGGGDFHCGE
                     WCRFCKAKAECRERAEANLALARYDFEEPPLLTDEEIAGILDKVDALTAWAADVKEYA
                     LQQAVSGTAFPGWKLVEGRSNRKYTSEAAVAAAVEGAGFNPYEKKLLGITAMQKLLGQ
                     SRFEELLAPYIEKPQGRPTLVRSSDKRPEWNTAKNDFMEEM"
     gene            12047..12622
                     /locus_tag="IW781_000014"
     CDS             12047..12622
                     /locus_tag="IW781_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HAQ1181041.1"
                     /translation="MSNNTNRVNNPMKVITGPDTRWSYANVWEPKSINGGTPKYSVSL
                     IIPKSDTKTVAKIKAAIEAAYQEGQAKLKGNGRSVPPLSAIKTPLRDGDVERPDDPAY
                     ANAYFINANSATAPGIVDADRNPVLTRSEVYSGVYGRASINLYAFNSNGNKGIACGLN
                     NLQLIRPGEPLGGKASAEADFATDDDEDFLG"
     gene            12643..12828
                     /locus_tag="IW781_000015"
     CDS             12643..12828
                     /locus_tag="IW781_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181042.1"
                     /translation="MTMFQTILLFAVLAIWLCISAVILISSIQSFLYDRKREKREREQ
                     AARDAEYHENRMKLLEK"
     gene            12894..14834
                     /locus_tag="IW781_000016"
     CDS             12894..14834
                     /locus_tag="IW781_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HAQ1181043.1"
                     /translation="MQTLSIDLETYSDQSLQKTGVYRYVESPDFEILLFAYSVDGGLV
                     QQIDLACGEKIPPAILAALEDDKVTKWAFNANFERICLSRFLGYPTGDYLDPDSWRCS
                     MVWTAYMGLPLSLEGAGAVLGLEKQKLTEGKDLIKYFCQPCAPTKSNGQRTRNLPKHA
                     PDKWLTFKKYNIRDVETEMSIQARLSKYPVPDSVWEEYHLDQEINDRGVGLDMELVRQ
                     AIQMDGRSRSELTQAMKELTSLDNPNSVQQMKQWLADNGVETDTLGKKAVAELLKTAP
                     PQLQKVLTLRQQLAKSSVKKYQAMETAICADDRARGMFQFYGANRTGRWAGRIIQMQN
                     LPQNHLDDLSEARGLVRAGGFDALEMLYEDVPDTLSQLIRTAFVPQENRKFIVADFSA
                     IEARVIAWLAGEKWRQDVFAEGKDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELAL
                     GYGGSVGALKAMGALEMGLSEDELPALVSAWRQANPKIVQFWWAVDHAVMDAVTRKTT
                     TKTHGIIFSARNGMLFITLPSGRSLAYVKPKIGENRFGGDCITYEGVGGTKKWERIDS
                     YGPKFVENIVQATSRDILCYAMKTLRCCSIVMHIHDEVVIEADRRMSLQAVCDQMGRT
                     PPWANGLQLRADGYETDFYKKD"
     gene            14848..15279
                     /locus_tag="IW781_000017"
     CDS             14848..15279
                     /locus_tag="IW781_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181044.1"
                     /translation="MSINKFNSEGYYDPTTYEALTNIEKEERALRAFRPIVYICSPYA
                     GEVAANVENARRYSRFAVDAGYIPIAPHLLFPQFLNDADPKERQLGLFFGNALMSKCS
                     EVWVFGEYISSGMEAEIRRAKWKNYRLRYFTTMCEEVTDYA"
     gene            15272..17611
                     /locus_tag="IW781_000018"
     CDS             15272..17611
                     /locus_tag="IW781_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181045.1"
                     /translation="MRELNIAYGNNRQAKRWVNKTIRFDNLKERLKVTIRTTESAEEY
                     AKMSRAQRDAAKDHGGFVAGVLKGGRRKVDTVESRSMVALDGDRIDTAFLDSYESLCP
                     YASALYTTHSSTEENPRVRLVFPLTRDVTPEEFVAVSRYLAQMLGIDYFDECSYQPNQ
                     LMYWPSTPANGSFVYKETDGGWLDPDAILTKHPEWTDPTRLPTSSRESKANTTAQQKV
                     QDPLTKEGVVGLFNRTYYPISKALETFLSDVYEPTDNENRWHLIASSSMAGVEIKEDK
                     FVYSHHAKDPAYLKLCNAFDIVRIHRFGDLDEKASYKAMCEFAMQQDEVKLLAASERM
                     ADAETDFSGSEDTDWQKRFQYEPRSTVLKNNLHNITLILQNDPQLQNIVFNQQLDGME
                     IKGDVPWKHPSKYWRDADDAQLISYVDSHYGTFSQRNYQIAVTKVADDRSYHPIREYL
                     AALPEWDGVSRVDTLLIDYLGAEDNSYVRAVTRKTLCAAVRRVQEPGVKFDTMLVLNG
                     PQGIGKSTLISRLAGEWFSDSLNLSDTKDKTAAEKLQGYWILEIGELAGLRKAEVETL
                     RSFLSRQNDIYRAAFGRRATPHPRQCIFFGTTNAESGYLRDTTGNRRFWPVKTPGGGI
                     KHSWELTTEDISQIWAEVLVLVKDGEKLHLAPDLEMLAKSEQREALESDEREGLVREY
                     LETLLPEDWDGMDLFDRRSFLAGVNNIGRVGTVARTRVCNMEIWCELFGKDQGSLGRA
                     ESNNLTAMLTKLGWVRKEKKERVKPYGPQFVFVPGDVPD"
     gene            17868..18401
                     /locus_tag="IW781_000019"
     CDS             17868..18401
                     /locus_tag="IW781_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181046.1"
                     /translation="MYEIDSAYVRRCQKRLREWGAPLSGWYCDYIYDVADEEEDPDHI
                     DLFTCELCDCSQVRFVHVMRHDEYFETVSVGCICAGIMEGDIFAARERERLMKNRAKR
                     KRNFPHRQWRKNWYGNYQLTYRGRKVFINNKGSNRYSVYVDGRTAWSYKGKPLDNFVL
                     AAYAAFELADPIERIRP"
     gene            18398..18679
                     /locus_tag="IW781_000020"
     CDS             18398..18679
                     /locus_tag="IW781_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAQ1181047.1"
                     /translation="MREKEIEKKLTQMVKKAGGICPKLVSPGFDGMPDRMVLLPNGRI
                     GFVEVKALGEKPRPLQLSRHRLLRRLGFPVYVLDDTEQIGGILDEIRTS"
     gene            18660..20024
                     /locus_tag="IW781_000021"
     CDS             18660..20024
                     /locus_tag="IW781_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1181048.1"
                     /translation="MRYEPHEYQKYAVDYIETHPAAAIFLDCGLGKTSITLTAIADLL
                     FDSFEVHKVLVIAPLRVARDTWTAEADKWDHLQDLICSVAVGTEAQRRSALMRRADIY
                     IINRENVQWLIDESGIPFDFDMVVIDELSSFKNHQTKRFKSLLKVRPKVSRIVGLTGT
                     PASNGLMDLWAEFRILDMGQRLGRFITKYRTDYFTPDKRNGQVIYSYKPLPYAEDAIY
                     RQISDITISMKSADHLNMPKLVSSEYTVRLSEDEQKKYTDLKQELVLSLGNAEITAAN
                     AASLSGKLSQMANGAIYDDGGETIRIHDRKLDALEDIIEAANGKPLLVAYWFKHDLTR
                     ISERLRKLHIPFSRLDDAASIRRWNNGELPVALIHPASAGHGLNLQSGGSAIVWFGLT
                     WSLELYQQTIARLWRQGQTSETVVVQHIVTKGTIDERIMKALSKKEHTQTALIDAVKA
                     DLKI"
     gene            20080..20307
                     /locus_tag="IW781_000022"
     CDS             20080..20307
                     /locus_tag="IW781_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181049.1"
                     /translation="MSNITIYEDLANAIILQAVKDYRMALKSLKANSRNRAAQADKAE
                     IERFFRSQWYSALTDVKGEMLIRSLQKEVDA"
     gene            20304..20714
                     /locus_tag="IW781_000023"
     CDS             20304..20714
                     /locus_tag="IW781_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HAQ1181050.1"
                     /translation="MTAKEYLNQARHLDALINCRLREIDYWRDLSSSVSGSNFEPHYN
                     PNKPTEAPFVRCLEKIDAIQRDVAEKVAYLVCLKETINAAIDRLASREEQLVLRYRYL
                     DNCSWEEISRMLNVSLRTVHRIHGSALQNFSVPN"
     gene            20848..21195
                     /locus_tag="IW781_000024"
     CDS             20848..21195
                     /locus_tag="IW781_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1181051.1"
                     /translation="MPRKPKRPCSYPGCPNLTDGQYCKEHEAAARRQYNKYERSADVN
                     KKYGRAWKRIRDRYAAAHPLCEMCLKEGRLTPVDEVHHIVPISQGGTHARDNLMSLCR
                     SCHTKIHHDLGDR"
     gene            21330..21878
                     /locus_tag="IW781_000025"
     CDS             21330..21878
                     /locus_tag="IW781_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1181052.1"
                     /translation="MPTKSNNTGGRGGARPGAGRKKSAVKEKAENGNPGGRRLEVLDI
                     PEVEGVDMPKPHEFLSAEQRDGSTLQAEEIYKETWEWLKKIGCAAKVSPQLLERYAMC
                     SARWIQCEEMTNRMGFLSKHPTTQKPIPSPFINIGINYMNQAVRLWNEIFQIVKENCS
                     TDYGEVSPQDDLMERLLRARKG"
     gene            21883..22668
                     /locus_tag="IW781_000026"
     CDS             21883..22668
                     /locus_tag="IW781_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HAQ1181053.1"
                     /translation="MFEKVNPCHPDKVADRIAGALVDAAYRKDENPRIAVEVLIGHGV
                     CHIIAESSVHIPLDEVYAIVKRIGGNLHTDYVEVPQDGHLANNQADGIRCGDNGIFKG
                     MPVTEEQKALCEIARSVYHTYHYDGKYILDGARLILCQSNARTQHLKEVYPHAEVNPL
                     GDWTGGTDVDSGATNRKLGSDMADSVTGGGLHGKDLSKADVSVNIYAWLKAQKTGKPV
                     ELCCAIGDDTVDSIPYAEIVETARRYILSLGGFEKFAEWGLVR"
     gene            22665..23903
                     /locus_tag="IW781_000027"
     CDS             22665..23903
                     /locus_tag="IW781_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAQ1181054.1"
                     /translation="MKTTTEMQLVPIAKLVPYVNNARTHSPEQITKLRSSLREFGFIN
                     PVIIDRDFGIIAGHGRILAAKEEGIKEVPCVFADHLTEAQKKAYIIADNRMAMDAGWD
                     EELLRVEIESLQGMDFDPLLTGFDEKELAALFDDGIEAKEDDFDVDAELQKPTFTKSG
                     DVWTLGRHRLVCGDSTKPEVYDILMDGTKANLVITDPPYNVNYEGSAGKIKNDNMAGE
                     KFYEFLLAAFQNMESVMAADASIYVFHADTEGLNFRRAFADAGFYLSGCCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKNGKHQWYTGRKETTIWEFDKPKKNGDHPTMKPIPLLAYP
                     IGNSSMANSVVLDPFGGSGSTLIACEQTDRICRTIELDEKFCDVIVNRYIEQVGSADG
                     VSVLRDGRTYSYEEVTDGTQ"
     gene            23890..25827
                     /gene="dcm"
                     /locus_tag="IW781_000028"
     CDS             23890..25827
                     /gene="dcm"
                     /locus_tag="IW781_000028"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1181055.1"
                     /translation="MEHNKLTLGSLFDGSGGFPLGGLISGITPIWASEIEPFPIRVTT
                     KRLPFMKHYGDVSKMNGADVEPVDIITFGSPCQDMSIAGRREGLDGSRSSLFYEAVRI
                     VKEMRCKTNGRYPRYIVWENVPGAFSSNKGADFQSVLEEVCSVKGYEIHTPRPERWPT
                     AGEIVADDFSLAWRIFDAQYWGVPQRRKRIYLVADLAGGSAGKILFESEGVSGYTPQG
                     FRSWQGTAGTSEKGSGAAGCVCLNDQGGNRMDVTDGVTCTLRAEVHHPPCVMESAGFC
                     TEHSAHSRSIGYEEETSPTLRAGTVPAAVYENHSQDTRYTKLEGIAPTVSSTYGTGGN
                     NQPFVVETPKTLKIRSGCEGGGKGALIQDNKSATLGCNNDQTVFVPKVYGICSKQSHA
                     MQSDNPHSGFYEADTCRCLDANGGNPSYNQGGMAVVAVQGSMIGRSDKNGPQGSGVNE
                     DVSFTLDATDRHAVAYGIDRATYNMGRNAKFGITVETEIEPTMVSKGPGAVGQPIYHS
                     SKSSFHTNFTDDDVTETLVATDFKDPPTVSKEPDYIVRRLTPTECARLQGFPDWWCSD
                     LGTANPTEDEVEFWTEVFETHRKVMGASSNPKSRNQIIKWLKDPHSDSAEYKMWGNGV
                     ALPNVCFVLSGIVYYAQLSGE"
     gene            25927..26637
                     /locus_tag="IW781_000029"
     CDS             25927..26637
                     /locus_tag="IW781_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="HAQ1181056.1"
                     /translation="MEIRYNVTGAKRKELVKVISDATGARAEYKFMPTCNYEIDYFTV
                     TKDGTLLFDDRADSEEVEQVLEAITAAGFECEAKGGEEQFFEEESEATDTAPQSETVG
                     LTVEIPLDKAAVGNLTKLLDAKGSLIKKALGVSELPIEIQEDRVTFPWFPELPDADAV
                     KAYTHFISALCEMSKNAKRVTVTEKAVDNEKYAFRCFLLRLGFIGSEYKVERKILLKN
                     LSGSSAFKNGGVDHAISE"
     gene            26621..26869
                     /locus_tag="IW781_000030"
     CDS             26621..26869
                     /locus_tag="IW781_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HAQ1181057.1"
                     /translation="MRFPSKEVVERIRKEFPSGTRVELLRMDDPQAPPIGTQGTVMGV
                     DDIGSIMVAWDNSSGLSVSFGEDLCRKVKNTSDGSKKV"
     gene            26999..27949
                     /locus_tag="IW781_000031"
     CDS             26999..27949
                     /locus_tag="IW781_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HAQ1181058.1"
                     /translation="MNEKTARQIAEMKNQTIGVEVEMNSITRQKAAKVAADFFGTGRY
                     ENTAGRNGYSTWSAWDAQGREWKFQKDVSIAGADEQKCELVTPILTYGDIETLQELCR
                     QLRHAGAKSDASRGCGVHIHIGAHGHTPQSLRNLANIMASHESLIAEALKLDRSRMSR
                     YCRTVDPNFLAKVNSRKPKTMAQLADIWYTSHGASYGRNQHYNDSRYHMLNLHATFTK
                     GTVEFRLFQFDEPTAERRGGIHAGQLKSYIQLCLALSQMAKDVRTASPKPQQNENPKY
                     AMRTWLLRLGFIGEEFATARDFLTRNLTGDTAFRHGRAAA"
     gene            28047..28523
                     /locus_tag="IW781_000032"
     CDS             28047..28523
                     /locus_tag="IW781_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAQ1181059.1"
                     /translation="MKEKRYYIAYGSNLNVPQMRMRCPHATILGTANLKGWELLFKGS
                     KTGSYLTIEECESGTVPVVIWEVTAADEAALDRYEGFPNFYYKKDINLQYKGIRTGKR
                     RTVTAFAYIMHEERPIGIPTNFYMRTCLEGYDTFYFDKNILIDAYEKCREVCGYEG"
     gene            28513..28713
                     /locus_tag="IW781_000033"
     CDS             28513..28713
                     /locus_tag="IW781_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181060.1"
                     /translation="MKDNIIRTAVCPLCGRTYHGAPALSREDNKTLICPDCGTRQALR
                     SIGVDTAEQEQIIETIHRHMQE"
     gene            28775..29164
                     /locus_tag="IW781_000034"
     CDS             28775..29164
                     /locus_tag="IW781_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181061.1"
                     /translation="MLLERICTHRKGKQRKTEETTMMNIYQMRNSFSLKEHNTAITRE
                     DFEGSFTRTRESVRFTFNGWDGKSYDGESRSAKVYRTSLPGYENTRFVKVGKALCYID
                     EDSSILEKATGEYQKEAEWLVDVLRSN"
     gene            29145..29501
                     /locus_tag="IW781_000035"
     CDS             29145..29501
                     /locus_tag="IW781_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181062.1"
                     /translation="MFSGATKKSEDTMMENTGIKRYEDYTENCENHYRLPNTSTMENL
                     SMKVTAGEGAVLKMGDKVLVTDHAWKGFIAGVYEFIETPEETGYSHIECRLNLIAMSG
                     ELFEDGGHAIAWAMQQ"
     gene            29612..31219
                     /locus_tag="IW781_000036"
     CDS             29612..31219
                     /locus_tag="IW781_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1181063.1"
                     /translation="MPMRKLKKYKPTRFMAKTSHYDKDAADYAVMFIESLCHTKGTWA
                     GKPFELIDWQEQIIRDLFGVLKPNGYRQFNTAYIEIPKKQGKSELAAAVALLLLCGDG
                     EERAEVYGCAADRNQAKIVFDVAVDMVRFCPALSKRVKILESQKKITYLPTNSSYQVL
                     SADVANKHGFNTHGVIFDELHTQPNRKLFDVMLQGSGDARMQPLYFLITTAGNDTNSI
                     CYEVHQKAIDIAEGRKVDSTFYSVIYGAAEDEDWTDPEVWKKANPSLGITVGIDKVQA
                     ACESAQQNPGEENAFRQLRLNQWVKQSVRWMPMEKWDACAFPVSEDDLEGRICYGGLD
                     LSSTTDITAFVLVFPPLDEEDKYYILPYFWIPEETLDLRVRRDHVPYDLWERQGVLMT
                     TEGNVVHYGYIEKFIEQLGERFNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDM
                     SPPTKELMKLVLEEKIAHGGHPVLRWMMDNIYIRTDPAGNIKADKEKSTEKIDGAIAT
                     IMGLDRAIRCGNDTGASVYDSRGLLFI"
     gene            31239..32618
                     /locus_tag="IW781_000037"
     CDS             31239..32618
                     /locus_tag="IW781_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1181064.1"
                     /translation="MGIFSGLFRSRDKPQNRTTGSTYSFFFGSSSAGKRVNERSAMQM
                     TAVYSCVRILAEAVAGLPLHLYRYKEDGGKEKAIYHPLYLLLHDEPNPEMSSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVIALYPLMPDRMTVDRDRDGKLYYEYTVSTDDAPTV
                     KGTVVRLKPSDVLHIPGLGFDGLVGYSPIAMAKNAIGMAIACEEYGAKFFANGAAPGG
                     VLEHPGTIKDPGRVRESWQSTFGGSGNANKIAVLEEGMKYTPIGISPEQAQFLETRKF
                     QINEIARIFRVPPHMVGDLEKSSFSNIEQQSLEFVKYTLEPWLVRWEQSIQRTLFSAE
                     EKKQYFTKFNVEGLLRGDYASRMNGYATARQNGWMSANDIRELENMDRIPAEDGGDLY
                     LINGNMLPLGNAGAFADTQPNDDGKEENPDEEVLEVDKQDGDESGESDGNSGENAVPE
                     RHHRRGKLV"
     gene            32503..33255
                     /locus_tag="IW781_000038"
     CDS             32503..33255
                     /locus_tag="IW781_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1181065.1"
                     /translation="MKKFWKWTNRTVTNQENQTETVERTLFLNGTIAEESWFDDDITP
                     QLFKEELMAGSGDITVWINSPGGDCVAAAQIYNMLMDYKGNVTVKIDGIAASAASVIA
                     MAGTKVLVSPVSMMMIHNPMTVAMGDTAEMQKAIEMLSSVKDSIINAYEIKTGLSRTR
                     LSHLMDAETWMDANKAVELGFADEVLKRSEVPEDMEPPAVSMLYSKAAVVNSLMDKIA
                     AKCKTNPKKTEDPKPQGRSVDSLYERLNLMKF"
     gene            33270..34472
                     /locus_tag="IW781_000039"
     CDS             33270..34472
                     /locus_tag="IW781_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1181066.1"
                     /translation="MTILELREKRAKAWEATKAFLDSHRNEKGMLSAEDDATYSRMEQ
                     EITDLGKEIARLERQEALEAELNRPVNNPLTGKPMNGKETAKTGRATDEYRQNFWNMM
                     RSKTPMPTVMNALQIGTDSEGGYLVPDEYERTLVEALEEENIFRQLAKIIQTSSGDRK
                     IPVVATKGTASWIDEEGAYLESDDSFGQVSIGAYKLGTMIKVSEELLNDSVFDLESYI
                     SREFARRIGAKEEEAFFTGDGSGKPLGILAATGGAETGVTAASATAITADELIDLFYS
                     LKAPYRRNAVWVLNDSTIKAIRKLKDNQGQYLWQPSLTAGAPDLLLGKPVRTSAYMPA
                     IAADAKTIAFGDFSYYWIADRQGRSFKRLNELYAATGQVGFLTSQRVDGKLILPEAIK
                     VLAQKSAG"
     gene            34494..34805
                     /locus_tag="IW781_000040"
     CDS             34494..34805
                     /locus_tag="IW781_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1181067.1"
                     /translation="MEALLSKVKANLILEHSADDELLKGYITAAVSYAESYQHIPASH
                     YTDNAMPATTEQAVIMLASHFYESRDGSTGGFFADNTNAAQQVWNTVNLLLRLDRDWK
                     V"
     gene            34808..35140
                     /locus_tag="IW781_000041"
     CDS             34808..35140
                     /locus_tag="IW781_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1181068.1"
                     /translation="MSFGKMNTFLSIVEKQFTQDEDGFKTETDVTVAEVLAYREGRHG
                     SEKWANMASFSTATDLFRFRVIPGVEITTDMRILCNGHTFEITSVEDVKGRGMYLEVL
                     GTEVKAGG"
     gene            35133..35564
                     /locus_tag="IW781_000042"
     CDS             35133..35564
                     /locus_tag="IW781_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1181069.1"
                     /translation="MAKATWKMPEDFLMKVSRLADKTDEIIPKVLEAGAEVVEDKVRS
                     NLQSVIGSGTKYDSRSTGELLRSLGTSPALQDRNGDFDLKVGFSEPRSDGSSNAKIAT
                     ILEYGKSGQPAKPFLKPARSASKNACISAMKAKLDEEVENI"
     gene            35561..35902
                     /locus_tag="IW781_000043"
     CDS             35561..35902
                     /locus_tag="IW781_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181070.1"
                     /translation="MSLLSELKTVIEAVGLPVETGVFSDEPPEEYVVVTPLADTYELH
                     ADNLPEYETQEARLSLFSRGNYLKRKKQLSKALLAADFTITDRRYIGHEDDTGYHHYA
                     IDVAKTYETEE"
     gene            35906..36520
                     /locus_tag="IW781_000044"
     CDS             35906..36520
                     /locus_tag="IW781_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1181071.1"
                     /translation="MATIGLDKLFYSKITEDEDGNETYATPASLAKAMTAELSVELAE
                     ATLYADDGAAEVVKEFQSGTLTLGVDDIGAAAASDLTGAVIDQNGVIISASEDGGAPV
                     AIGFRAKKANGKYRYFWLYKVKFGIPATNLTTKGESIEFSTPTIEGTVMRRNKVDGQG
                     KHPWKAEVTEGDAGVSASTITNWYQEVYEPSYAAAAAASLDGEG"
     gene            36534..36914
                     /locus_tag="IW781_000045"
     CDS             36534..36914
                     /locus_tag="IW781_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181072.1"
                     /translation="MDERTAMVTIGGVEYEMLLTTRATKQIAGRYGGLENLGEKLMKA
                     ENFEMALDEIVWLITLLCNQPILVHNLKHPEDKKPELTADEVELLTSPMELTDYKDAI
                     MEAMYRGTKRNVESEPEGKNTAAG"
     gene            37228..39888
                     /locus_tag="IW781_000046"
     CDS             37228..39888
                     /locus_tag="IW781_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1181073.1"
                     /translation="MTDNLGLKIGIEGEKEFKKALSEINQSFKVLGSEMKLVSSQFDK
                     NDKSVQALSARNTVLNKEIEAQKNKVETLRAALRNAADSFGENDRRTQNWQIQLNNAE
                     AALNSMERELSDNERAIEALSREETDAADATERLSQEIARQEDALAGMKRAYSNAVLQ
                     YGKGSSEAKELEGRISQLSGELRENRERMKDAGDVAEDFGDSLEDASSGADKLGSGLS
                     VATVAMGNLISSGIQAALSGIQELGSAIWNLDEATEEYRVAQGKLTTAFEAAGYSGDT
                     AQKSYTEFYKILGDTDTATEASQLLAQLAENEQDITKWTNIAAGVYGTFGDSLPIEGM
                     IESANETAKVGQVTGSLADALNWVGISEDAFNEKLAACSSESERNRLIMETLSGAYDE
                     ASGAFYRNNEALVASREGQAQMDETLAGLGETISHVKNSLRAEFLPAISEVISAFTDM
                     VNGVDGADEAFAGAITGLVNTAVSMLPQFVNTGMQMLTSLLSGIIQSLPAVMEGAAQI
                     IVTLAQGIAAAVPTLIPQIVLVVTQIVQTLIENLPMILDAALQLIMGLAQGLLDAIPV
                     LIAALPAIITAIVEFIVGAIPQIINAGIQLLTSLVSALPEIITAIVAAIPQIIDGLVT
                     AILGSIPQIIDAGVNLLISLIQNLPTIITTIVGAIPQIISSLVNAILNSIPQIIQAGV
                     QLFVSLIQNLPAIIVEIVKAVPQIIAGIVNAFTSSMGQIVNIGKNIVQGLWQGIQSLA
                     GWIWDKVSGWISGIWDGICSFFGINSPSKEMAWVGEMLGRGLAGGIEDSAGEAVSAAE
                     DLNNGILGVMNGLAADMQSAVPSNFVFDAGGTLRSAAGGMGSGGSSFGALITIQQMIV
                     RSEDDIRRISQELYNLIQTGSRAQGRFSTA"
     gene            39903..40679
                     /locus_tag="IW781_000047"
     CDS             39903..40679
                     /locus_tag="IW781_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HAQ1181074.1"
                     /translation="MGFSYNDITSKSMGLKARLTSWQVSGRLRNFTTTVPGKYGVTDF
                     GADFDYREIVVSCSIFPRHSFPALVSTLDDIAAWLDPVGGLKQLILDDVPDRYFMARL
                     NAAAECERLLRSSGSFELTFFCPDPFGYAIEDETFSITAEGSHTITRHTGNMESNPIY
                     RIEGEITSGVGNHISITTNGQELKIVNATLAAGETLVVDTDRMTAYVEDENGVTVRNG
                     LPYLEELNFPTLSVGDNTVSVAVSNAVFTGLEIQARSRWR"
     gene            40686..43211
                     /locus_tag="IW781_000048"
     CDS             40686..43211
                     /locus_tag="IW781_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181075.1"
                     /translation="MALKTILNQQTDFTGEFPVEWAKDGLWRMNESAPDENNNLLDSS
                     GAGRPAFINNWSGTTASMRSGQKGNYFRFNINNPATEQTYLKVANDGSIFAELGGRIL
                     CGGWMNPTTYSVGNTYCPIFNTRYGPGQPIFYLSLFRGRPRVMLYDNTGSLILDETVT
                     PPFSLVNGGWYFIACLIEPDNKTAQYVVGDRESGTVWASEVFTFTGELNRSCTADLIL
                     GMHADSYWYAGGLDDWFLDCDTQLTAEDLMDYFRSSLMTNAGDTSGSVDGITEPGAVT
                     LRASSGVYPSEGVLTTAAAECNLSGTGRVSVTSEYISGTTSVSLVETSTSDDLTDWSD
                     WAAVPADGRLTSPNRAYIRFRVTLTTSDTSRTPKLIDIRLYDIPKAPYEKIGYARPVV
                     LDGNGAWEAVLENAYDIIVTGEINGEDTLSFKIPYRDGKRGYIDSEKKIQIVDDVYKV
                     RTVTDTRDADGSAVTEVYAEAEFYDLTFSVRKEERTFEAEYPETAMAYALEGTEWSVG
                     TVTVRTKRTWTSTEKNALSILRNTADLHGGDLVFDCPNRLVHLLTVNGRDSGALFAYR
                     KNMKSIQRVVDTRELVTRLYAVGAEGMTFADINGGKAYVEDFTYTNEVRISTLDCSSF
                     TNPYQMKEYAEMRLADYAKPTISYVLNAMDLSVLTGYEHEAWELGDYVRVEDKELGLS
                     VTTRIVRREYNLQEPWNTVLELSTTLKNLGSSASEWDNAADSLEGTSMVSNNDIREMV
                     PFNLLRNSRADDGLAYWVSSGFEADSENGASGTASFKAEGVAGMTKSLSQTVYPANRS
                     SYTLSAQIGSENLEKLSDDAQVGIEVVIEYEDGSTESRFIDLY"
     gene            43227..43424
                     /locus_tag="IW781_000049"
     CDS             43227..43424
                     /locus_tag="IW781_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181076.1"
                     /translation="MAYLSSTSAKITPENYSARVKSITVRVCVTNCAGILYITDILLQ
                     AGAVATGWVGHPCEMKWTLDG"
     gene            43417..44001
                     /locus_tag="IW781_000050"
     CDS             43417..44001
                     /locus_tag="IW781_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181077.1"
                     /translation="MGKVAFIRLAEVINRKQDMRVVSVTVKPTITDCSGTIYFTDLML
                     QEGPALTGYTPHTEVFLQKFRENGEVKAPVWFNGVVRGEETVILFNLGETSAGLDVHI
                     YPKSDLEAGAVSLCQGVGGQKVSFPNAVPAEADLALLASTRQCTKNGSPEKKEGFYQY
                     SAAWDSKHKVTLPEGKTARVLFEMQEMQDGGEPI"
     gene            44001..46469
                     /locus_tag="IW781_000051"
     CDS             44001..46469
                     /locus_tag="IW781_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1181078.1"
                     /translation="MDTLKGKQIMVWTFMGNARMYEALRDYGDRISQIGLFSFKVRAT
                     GEIYESGVAISDMLTYINKWPHIKWLLTVANDGANSIFRALRDNTDGAQDRFLSEIVR
                     IMEKYPWCDGIDIDLERGDGYSTHTESTAMFRNIYNTVKDYDPAKHTNICLPGMTSVN
                     GSVGGENWCVYGDLNAYCDTASIMSYGMAWAGSAPGPVSPRSWLEGIYDYATKVMDPD
                     KIFLGMPAYGWNWQIYDTPENLGETYRGVSNTYYAARYWMTGAYNFTGDAPPQPFLPI
                     VAYWDDYDKVPYAFPHVYDYMEGADAVSREYPQLADTYNRRRYLTAYGKEQKTEFGTI
                     FIDRDADGYSSASGIVSIENGIATLGDNGSVTYSFSVNTAGTYDVAVRLCYPFWDKNG
                     IYAALDGSTKHFTESRLWWPHWRSTFWASLASGVTLSAGTHTITISVDAKGVQFYGFR
                     VCSAFSEEPTAGEATFALAPRSFKDVDGNMAVPDKGFKLTLEMLRRKPDSALVWYEDF
                     QDYGVLETDYWTVRSGSFEVWRSDEYSMERVYSQLEGHGELAWQYDGFSELHLRARLA
                     FPANGSGRAGVFCGSLFCCLNYDTQAVELYNGSTLLSSYSQEIARTSSADLRVNPSMY
                     TVEMRVRGNRVRVYSGSSYTLRFTATASGFSGGYAGYRSDNTTVCELLRLGDAWTYEP
                     YERFDVEMPDGSFKSYGRISRFNCTWDEEFQVFTLTSDVEETSTRSEDISLDYDFFHS
                     DLLEISCGGNYTARIIPKDINIWISRLFLGDADGFSILYYQDVDSLVYWANQAAYRWK
                     LRGMCMWSLGQEDMRLWEWLPKQI"
     gene            46557..46976
                     /locus_tag="IW781_000052"
     CDS             46557..46976
                     /locus_tag="IW781_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1181079.1"
                     /translation="MKEFWNVIQMVFTAVGGWLGYFLGGNDGLLIALVVFAVADYITG
                     VMCAVSDKKLNSQVGFKGICRKVLIFLLVGIAHILDVQVIGTGSVLRTAVIFFYLSNE
                     GVSIIENAAHLGLPIPEKLKAVLEQLHDRAENEKEDE"
     gene            46979..47917
                     /locus_tag="IW781_000053"
     CDS             46979..47917
                     /locus_tag="IW781_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1181080.1"
                     /translation="MAYTNSSLVSYTKLSPNHSGQRTHSIDRITPHCVVGQCSVETLG
                     SIFLPTSRQASCNYGIGVDGRVGMYVEEKNRSWCSSSSANDQRAITIECASDTTEPYA
                     FKDVVYQKLITLCVDICKRNGKKKLLWLGDKDKTLSYESKSDEMVLTVHRWFANKSCP
                     GNWMYARMGDLAEKVTAQLGGSADTPTVTTQLYRVRKTWSDSKSQKGAYKILSNAKKC
                     ADANPGYSVFDVNGVNIYTSKTIAAEVPFLVKVSISDLNIRKGPGTDYAKTGKFTGKG
                     VFTIVEVKSGKGSTAGWGRLKSGAGWISLDYAAKIK"
     gene            48038..48460
                     /locus_tag="IW781_000054"
     CDS             48038..48460
                     /locus_tag="IW781_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1181081.1"
                     /translation="MTNQQKEQITALRSQGYGYATIAKAVGLKKDTVVAFCRKMGLTG
                     TKAADNSRIELDAGFCLQCGALLTQTPGRKRVKFCSDNCRTAWWNAHPEKVNRRAVYH
                     FTCAHCGKPFTAYGNAKRKYCSHACYIADRYKGGDGHE"
     gene            48673..50439
                     /locus_tag="IW781_000055"
     CDS             48673..50439
                     /locus_tag="IW781_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1181082.1"
                     /translation="MRIVNKIEAKTPQIPHRKRVAAYARVSMESERLQHSLSAQVSFY
                     SSLIQSNPAWEYVGVYADNGITGTKAEAREEFNRMIADCEAGKIDIVLTKSISRFARN
                     TVDLLNTVRRLKELGVSVQFEKERIDSLTEDGELMLTLLASFAQEEIRSLSDNVKWGT
                     RKRFEKGIPNGRFQIYGYRWEGDHLVIHEEEAKIVRLIYDNYMNGLSAETTEKQLAEM
                     GVKSYKGQHFGNTSIRQILGNITYTGNLLFQKEYVADPISKKSRINRGELPQYFVENT
                     HEAIIPMEVYQAVQAEKARRRELGALANWSINTSCFTSKIKCGRCGKSYQRSNRKGRK
                     DPNANYTIWVCGTRRKTGNAYCQNKDIPEQMLKDACAEVMGLDTFDEIIFSEQIDHIE
                     IPAPNEMIFYFKDGRIVPHHWESTMRKDCWTDERRAAKGRYVQEHQLGPNTSCFTSRI
                     RCDSCGENYRRQRSRHKDGSFDSVWRCASGGKCQSPSIKEDALKNLCADAMGLEEFSE
                     TVFREQIVCIHITAPYQLSIRFFDGHTFETAWENKRKMPRHTEERKQHMREVMIQRWR
                     EKRGESNDNTCDDKPIHGNADQ"
     gene            50378..51949
                     /locus_tag="IW781_000056"
     CDS             50378..51949
                     /locus_tag="IW781_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1181083.1"
                     /translation="MAKVTTIPATISRFTATPINEKKKRRTAAYARVSTDSEEQLTSY
                     SAQVDYYTNYIKSRDDWEFVSVYTDEGITGTNTKHREGFKRMVADALAGKIDLIVTKS
                     VSRFARNTVDSLTTVRQLKEKGVEIYFEKENIWTLDSKGELLITIMSSLAQEESRSIS
                     ENCTWGQRKRFADGKVTVPFKRFLGYDRGPDGNLVLNKDEAVIIRRIYSMFLQGMTPH
                     GIAARLTADGIKSPGGKDKWNAGAVRSILTNEKYKGDALLQKSYTVDFLTKKKKVNEG
                     EIPQYYVEGNHEAIIQPEVFELVQQELERRKSSRGRHSGVHLFSGKIRCGQCGEWYGS
                     KVWHSNSKYRRVIWQCNHKYDGEEKCSTPHLTEDEIKAMFVSAANKLIGKKAAIISPL
                     RNSLDVAFDTSALETEVAELQDEIMVVSDLIEKCIYENAHVALDQTEYQKRYDGLTTR
                     FDTAKARLEEIEAALADKKSRRAAIDAFLDTLAQADPMEKFDPALWCGLIDYVTVYAR
                     DDVRFAFKDGQEIKA"
     gene            complement(52048..52782)
                     /locus_tag="IW781_000057"
     CDS             complement(52048..52782)
                     /locus_tag="IW781_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAQ1181084.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFEIILLSLIQCRGIIKKCAFAV"
     gene            complement(53292..54017)
                     /locus_tag="IW781_000058"
     CDS             complement(53292..54017)
                     /locus_tag="IW781_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181085.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(54171..54497)
                     /locus_tag="IW781_000059"
     CDS             complement(54171..54497)
                     /locus_tag="IW781_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181086.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(54534..54899)
                     /locus_tag="IW781_000060"
     CDS             complement(54534..54899)
                     /locus_tag="IW781_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1181087.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(54936..55151)
                     /locus_tag="IW781_000061"
     CDS             complement(54936..55151)
                     /locus_tag="IW781_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1181088.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            55280..55615
                     /locus_tag="IW781_000062"
     CDS             55280..55615
                     /locus_tag="IW781_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1181089.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(55691..57688)
                     /locus_tag="IW781_000063"
     CDS             complement(55691..57688)
                     /locus_tag="IW781_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181090.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(57786..58061)
                     /locus_tag="IW781_000064"
     CDS             complement(57786..58061)
                     /locus_tag="IW781_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1181091.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            58293..59027
                     /locus_tag="IW781_000065"
     CDS             58293..59027
                     /locus_tag="IW781_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1181092.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            59066..60208
                     /locus_tag="IW781_000066"
     CDS             59066..60208
                     /locus_tag="IW781_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1181093.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(60303..61694)
                     /gene="sufB"
                     /locus_tag="IW781_000067"
     CDS             complement(60303..61694)
                     /gene="sufB"
                     /locus_tag="IW781_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1181094.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(61699..62169)
                     /locus_tag="IW781_000068"
     CDS             complement(61699..62169)
                     /locus_tag="IW781_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1181095.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(62156..63391)
                     /locus_tag="IW781_000069"
     CDS             complement(62156..63391)
                     /locus_tag="IW781_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1181096.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(63388..64674)
                     /gene="sufD"
                     /locus_tag="IW781_000070"
     CDS             complement(63388..64674)
                     /gene="sufD"
                     /locus_tag="IW781_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1181097.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(64687..65460)
                     /gene="sufC"
                     /locus_tag="IW781_000071"
     CDS             complement(64687..65460)
                     /gene="sufC"
                     /locus_tag="IW781_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1181098.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(65657..66505)
                     /locus_tag="IW781_000072"
     CDS             complement(65657..66505)
                     /locus_tag="IW781_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1181099.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(66529..67215)
                     /locus_tag="IW781_000073"
     CDS             complement(66529..67215)
                     /locus_tag="IW781_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181100.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(67208..68242)
                     /locus_tag="IW781_000074"
     CDS             complement(67208..68242)
                     /locus_tag="IW781_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1181101.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(68637..68975)
                     /locus_tag="IW781_000075"
     CDS             complement(68637..68975)
                     /locus_tag="IW781_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1181102.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(68977..69333)
                     /locus_tag="IW781_000076"
     CDS             complement(68977..69333)
                     /locus_tag="IW781_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1181103.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(69339..70526)
                     /locus_tag="IW781_000077"
     CDS             complement(69339..70526)
                     /locus_tag="IW781_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1181104.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(70717..71562)
                     /locus_tag="IW781_000078"
     CDS             complement(70717..71562)
                     /locus_tag="IW781_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1181105.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            71796..72761
                     /locus_tag="IW781_000079"
     CDS             71796..72761
                     /locus_tag="IW781_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1181106.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        18942 a        18109 c        17948 g        17907 t
ORIGIN      
        1 aatttttagc atctaattta acttcaattc ctattataca aaattttaag atactgcact
       61 atcaacacac tcttaagttt gcttctaagt cttatttcca tactcgctct taatacaata
      121 tagtgtacct cgtaatcttc ttgggcaaga gctttccatg gctccaaaaa ccatggcccg
      181 acaatcccat ctacaattac atacaaattg aaagcaaatc atcttccgtg gcttttaccg
      241 cttctcgaaa tgtttgaaca ctccacactt ttcgcctagt ctttggaatt tttggtaacg
      301 tagccaaaga cgcagggttt cttttgtttg tatcaatata ctcccagcgg atagcttgat
      361 ttaaggcaca gcggataata tcatgtattt ttttgatgtt cgctggttga acacaacgac
      421 cagttgcttt tctgtttgcc cgtggtactt ctggaacgct caataaatca ttgtagtgat
      481 ttgtgattaa tcacttcctt tttcgcttaa cagctcccgc attatgagaa gcctttcata
      541 cataaattga aggtattcat cgtttgtcca aatatcgcca tactgctttt cctcaaacga
      601 agagctgtcg ctcgtggctt tgccgatacc tttaaggtca attttctttt tatagtcggc
      661 cttgctatcg aacggagggt caatgtagat gaggtcaatc ttcccccgat aatctttcag
      721 cagatggctc atgacctgga gattgtctcc ccagaatatt ttgttaatcc atccgttctg
      781 ttcctcaccg taagactccc gtagctgcgc agggtaatac tgcgtagagc ggtaggggcg
      841 ctttcccgtc cagcgaagtt cagggaaacc cttgattgtg ggtctctgtt caaattcaaa
      901 agtaagctgc tcatccattt tcgttaatcc tcctgaatag tttcggttct taattgatgc
      961 tcgatatccg acatatccaa tggaatgtac tgcccattcg cagtaagatt acgtccgaat
     1021 atactgccaa agattgtttg attctgcatc acaatctgcg gattgttttc caaataagca
     1081 ttgaagcctt caagcacatt cggaacagag agtttttcca gtaatgtggc tctatcgact
     1141 atccctgcat tgctgagatt ttcaatgcct tctgaaacct ttttcctgcc atcagcaatt
     1201 tccgccatgc cgacaactat cttcattaac tcctgtattg cttcttcttg agttagcgat
     1261 cttcgttcgg catcattaga ctgtacagca tctgttgaaa ccacagtgtc tgtaggttcg
     1321 attgccattt gtatattttc ttcctcctga agattgactt gtgctttgcg ttgttttgct
     1381 acctgtgctt cggttttctt taccttatgt ctgtcccata atttctttat tccgtagcct
     1441 gctccgataa tagcagccac aatcaaaccg cctattgcag cagaacctgc atcaattccg
     1501 tttgacggtt cttcgtattc atcgctatcg tccaagcgac tgatatcaac cttgtttgct
     1561 atggagccgt ctgtccgtct tgcatagaaa tcatccgata aattaccttt ttggtaatcg
     1621 tcgtagtcat cgtctgtaag accgattttc agacctctct ctgggtcata tatgtccggc
     1681 atcgttattt cctcctgcag tagaatcgga agtcacacgc attacagatc ttcgggtctt
     1741 cggcgcagcg gcggaaatct ttcttcatga ttttcttgac cgtatcatcg aataccgcca
     1801 tagttccttc cacagcggac ttcgtgtata tgtatgtaat agtcggcaca ccgctgtcct
     1861 cgccggtata gtagatgtgc atcttactaa ctttctgtcc ggtacgttcc tctacgaggt
     1921 gagcataaat atgcagctgc cttcgatatc gttcaagctg ctcccgatct ttcaccatgt
     1981 caggttttcg ctgagattta aaatccacaa tctctacggt atctccctca ccacgaatta
     2041 ggtcaacctt cccctcaata atgtagtcag gcttgaccag cgaaacatcg acctctgcct
     2101 gttgtatctg tgaccagtca ccgtgctgcc gttccacata acgtagcacc tgtttcaggg
     2161 ccgcctcacg ctggggaccg gcgaggtaag tatgctcggt ctttgtcagc gacacatagt
     2221 tagagtcaaa ccaccggctc acattctcct cagtgattgt ctgttcctca tgccgcaggg
     2281 cggcgcggtg tatgtcctct atcgtctcat gcacgagcgt accgaagagc atagcgttcg
     2341 cacgaacggg catgaattcc agttccttgt agaacttata ttgaagggcg caggtctcgt
     2401 aaaccgtaat atgcgatgtg aacgagtagg tgttctttat gttcacgccc tttatcggct
     2461 tgaaatcaaa ctcccttata tcaaaaccgg ggctttctac agattgcagt tccccgtaga
     2521 tgtctttgaa gtagttgcta ggcgttcgct tatcctcgca gcaggtcagg atcagcaaat
     2581 cctgcgcacg ggagaaagcc gtgtagtaga gccgccagaa gtcaaagtac ttcgtgacat
     2641 cgtaaggttc aaacgccgga cgcttgaagt atctctcctc aatctgcatc atgaggtcgt
     2701 tcgtgttttt cctcggcaca ttcgcaaggg aatccacaag gacaatagga aactccatac
     2761 ccttggactg gtgaatggta aggaatgata cacagccgct cggtgcatac tctgaatcgt
     2821 cctcgtattc cgtaatgccg ccgtcaaaga gaagcctgag atacatatta aaaagcaatt
     2881 ctgtattctg atcaatacgg cgtttcccac ggtattctcc tgcgtccaaa acgtccacgc
     2941 ggtggaggta ctcgaactta ccaataatct gcgtcagttt tgcaaggttt cgagtcggac
     3001 ggatatcgac cacgccaaca tccatttccg tatcaagaat ccctgcaaac ggctgaaatt
     3061 caaagagctg gtacatcaga ccgcaatagg cgtagtccgt tgctcccttc aagccgacat
     3121 ggtctttacc tctgcgccgt atccacttca aaagattgga attctccggc tgtgtgaggt
     3181 actcatttgc tgccatgatg caattgcggt agtaggtcag atgcgctggc tgcaggaagg
     3241 tgtaatcacc gttctccaag ccctggatat agcgcggaaa catgagcatg agacaaccga
     3301 gggcaagcct tatctcgtcg cgctggaaga acatatccga acgcggtgaa tatacattta
     3361 tatgattctc ctcaaggaag cgggcaagtg ctgttactcg cggatgcttc acggaattga
     3421 agagaaacgc aatctgatta tagtccgtaa gtttgccgga ggctttcaag ttgttgataa
     3481 actgcagtat cttctcgtgc cactcatccg tatcgtccac gcctgcgagt ttcacgactg
     3541 cgggactgtg gagagtggtt ttctcgtgcg gttcgatcct tttatcgtaa cggaagttat
     3601 cccagcgaaa cttgaacttc gcaccgtctg tggtagccat ccactcgttg tagaaatcaa
     3661 cgatgtcgct gttggagcgg tagttgatga ccagtgggat gacgcggcat tcgccgtccg
     3721 caaacttctg cggaaactcc aggatgttac ggatggtggc acctctgaag cggtacaagc
     3781 cttggtcatc gtcaccgacc acacagatgt tcttgcggtc tcctgcgagg aggaacacaa
     3841 gctgctcctg gatgaagttc gtgtcctggt attcgtccac catgatgtgc gtgaccttgg
     3901 agcgcagttc ctccaaaatc ccggcgttgt cccggagcag atgataggct tcaatctgaa
     3961 tagacgagaa gtccatcaga ttgccctcag ttaggacatc gcggtactcc ttcaacatcc
     4021 gtcccagagc cgcaatcgac aggtcagtat cggacatcag ttcctctggc gtcaccaatt
     4081 cttcggagag gttattcaca tagttgcaga tagcttccga ctgcttccat gcgccgccgt
     4141 tcggcagagc aacctctacc tcctggatat tacggaaacg gtggatattc tggaacacca
     4201 tgtactgctg gtcgaacgca tccaggaggc ggtagtttct gcgaagtctt gtaaattcaa
     4261 gatgctcctt aaggatacga agacagagtg aatgaaacgt gccgatatac atctcattga
     4321 tgttcgccac gatgcctcga tccgacagtt cattcgtgat tcttgtaatc agttcctttg
     4381 ccgctttctc cgtaaaagtg gcaataaaaa tgctttccgg ctttacgccg cattcctcta
     4441 tgagatagat cgctcgttgg acaagtgtgt aggttttgcc tgttccaggt cccgcagtaa
     4501 tgaggacagg accttcggca gcagctatcg cttttcgctg tccctcgttt gcatttccaa
     4561 aattaaacat attggtgcct tacatccttt ccggcaatca atcttctgat gcgggactcc
     4621 catccggcac gacttccatg atatcagata tgtcgcactg aagggcagca catatcttca
     4681 agagaaccgc cgtgctgaca ttttctccgt gtgtgagctt cgccacggtg gaggaactga
     4741 gctgcgccgc ctccctcaaa tcccgcttct tcatgtcctt atcgatcagt aacttccata
     4801 actttttata gctgataacc atgcaaatgc tcctcaaatc tgcaaccctt tgtttccgtc
     4861 aagggcgcat ttctcccaag taacttaaca gttacatatc agtatagcac atttcgtttc
     4921 aaaagtcaat tgatgagtgc gagaacgcga aattttattt tccaaaatcg gagttgggat
     4981 atggatggta atttggattg tgttcaaagg tgttcaaaga atttcggaaa acttattgac
     5041 atttgcctcg aaatagtgta aactaaaagt tacagtccga cactatgact gagaaagagg
     5101 aatcgataga ctatgaaagc cgaattgatt gcaaatatta tcgctgctca ttgctccggg
     5161 gacgagagtt ccttcaagac cgctgtggaa gctttggcgg ctgacgagga taagaaagga
     5221 aacagccgtg tttccagtat gctcctcgat gcatataagg gaaaaaacat tactctgatg
     5281 aagaagcctg aggtcgggca gcctgccggg ggaggctttg ccgtccaatc tgcaggaggc
     5341 agttccttca ctccgagaga caaggacagt ctcctggaac tgtacgacat tgtccactcg
     5401 gacatttctc tctccgatgt tgtcctgcca gagaatcagc ggaaactcct cctgcagatt
     5461 ttggaggaac gccagaacaa cagtaagctg gcaaagcaca atctccctcc cgcaaaccgt
     5521 ctactactct gtggacctcc tggatgcgga aagacgatga cggcaaatgc catagcgcac
     5581 gaactgaatc tgcctatggc ttatgtgagg atagacggtc ttatttcctc ctaccttgga
     5641 cagactagtg tgaacttaag gaagatattc tccagcgtgg gcaaccaaaa cattgtcctt
     5701 tttctggatg aattcgatgc catcgccaag aagcgcgatg acggacacga gatgggagaa
     5761 ctgaaacgcg tcgtaacggc tctgctccag aacttcgata atatgccgcc gaatgtattt
     5821 ctgattgcgg ctaccaacca tgagcatctt cttgatcccg caattttcag acgattcaac
     5881 tatactatca atatcggata cccggatgca aaacagcgga gcgaactaat tcagcggtgg
     5941 tttaaagact atactgtaga aaccaaggta gatatacaga agctgactga ccttactgca
     6001 gaccgcagcg tggcacagat aaaggaactg gttatctccg ccgctaaacg ctatgtgacc
     6061 acagataagg cggttacgat ggaggatatg atagaactgc ttatccaaca gatgacgaat
     6121 aattcaaacg gggcaggcgt ggtggatgcc gccgccgatc tgaaagcaaa gggcgtgagc
     6181 gtccgtactc ttgcgaaagc aatcgggatg ccacataaca ctcttagcta tcagataaat
     6241 aagataacta agggcaaaga ggtggatgaa taggtatggc agatgatttc agaccccatc
     6301 tcctcattac tgaggatgat gtagaggcta ttgagaataa aaagcaggct cggagtaaag
     6361 acctgggatt ggatcggatg aagcatggaa cgaaactttc cacaggtctt caagaaattg
     6421 tgtctgccta tacccgcgtg caagggacgg attctcttcg tgatgaggat atccgtctgt
     6481 ttgaagttgt gctgcaagag ggtgagaaat tctcaaacaa aacacttcgt gaattcttgg
     6541 aacaggaagg aatgaccttc gccaaagtaa aagattcacg ccacgccatt gtatcatcat
     6601 ccaagtccaa gtttgattcc ctacaacaaa gggtaggcgc ttaccgtgat ggcaagcgca
     6661 gtaacaagaa atttcagtac atagatgatt ttcaatttcc ggatggaatg ggaaaacagg
     6721 ctccctcgat aaaagaactg cttgaacgtg aggctacttt cccacttgat gttgaaatca
     6781 tggaacaatt gctcccaaag ggtaccgatc cgcaggttca ggtccgtgcg gaagagaggc
     6841 ttattgcgct tattgagcag aatcaaggta aaatccaagc gaagccatat aaactttctg
     6901 atggaacacc tattgtccgt gcggagattc cgcttggtaa acttgaagag atttccaggg
     6961 atactattgt cagtcatgtt gctccgacag gcttctacgc cacatctccg atgtatacgg
     7021 ttcctgcgca ggcgcagatg tcgttgaatc caaatgtttc gatagatgag cttcctatcg
     7081 tggctgttct tgatacggga gtggatttcc ctgcggaact tgaaccgctg attgtcgagc
     7141 attgggttcc tactggagcc gctccggggg ataagaaaca cggaacgaat gtggcgagca
     7201 aggtggcgtt tgaaaatctc ggcgaacagc ttgcgtccgg cgtactcaca ccaagagcaa
     7261 gaatcatcga ctgtaacatc cgtggactcg atcccgactc ccataagcct gatcgtcccg
     7321 accttatttg taacagcact atgattgcaa gaatcaagga ggcagtcctg cgctataaag
     7381 acataactaa aatcttcaac ttctcatcaa gcgaagagac tcctatccag ggtgacgaaa
     7441 tcagtattct tgggtacgaa ctggatgtcc ttgccattca gtatggtgtg aaatttacca
     7501 tttcagccgg caatcattat ctgtatcgta gccaggactc ccttgaagac atcctgcaag
     7561 atgatgacaa tagaatagca gcaccagcag attccatgct gaatatagca gtcggagcta
     7621 ttgtcggtgc ggaacacaag gaaggtttga gccgccaata tgatgtggct ccttattcga
     7681 gaatcggtcc cgggttccga ggtttccgca aacccgatat cgtatctctt gcgggaacga
     7741 tgaccaaggc tgacttcgta ccgccagatg aatatgccat gatgattgcc agcggcggtc
     7801 aatgggcatt tgaggcaggt acaagtttta cggctcctac tgttgcagga gaccttgcgg
     7861 agatatcaca gtctgtgccg aatcaggata tccttatgtc ggaggcactt ctctatcacg
     7921 gcgccgagat gcctatcagc gaggttgcaa agaaaaaaat cacaagggac gaaaatgcct
     7981 tctatggcaa tctgtacggc agaggcatag caaatcctct tgtgagtatg ttttctactg
     8041 ctcatcgcgt gaccttcctc cacaggggta cgatgaataa gaaattcaag cagcacgcca
     8101 agtttctaat gccgagtatt tgcgatacgc agatggatat gagtaagagg gataaaaaaa
     8161 tacgagtgac agtaacctgc gtcacacaac cgcctgtcga taaaactaag ggcgctgatt
     8221 atctcggagc atatgtcaat gcgtcccttc attcaagaaa cagtaaggat aaccttgtta
     8281 cgaataatcc gtcagaatca gatggtcgca aggattggga tacctgtttc cacttcgagc
     8341 aggaattttc aagttttcac ggtggtgatt gggaagtatg gcttgaactt catactcgct
     8401 atgacgtgga ggatgaccag gaggttgact acgctcttgc cataacgata gaggatttaa
     8461 ccaagtcgct gcagctatac gatgctgttg tgaatgaagc acagaaccgc ttcccagcag
     8521 ttcagcttgt acgtcttcct gttcgcacat aattaaaaag agaacgaggt gccctctttg
     8581 ctaagaagtc acctcgttaa tctttaatct ttgcgtcggc ttttcaagaa tgcaatatac
     8641 tggttgacct gatcaatctc ttcttgactc aagtcttccg tatcctttat gccggtagct
     8701 actgtactgc tttctgtttc cgcagtaggc tcatcaatat atccagcagc cgccatcaaa
     8761 tcctcatagg gcacaccgag cgcggacgct atggagcgaa gaacctgcgg agaagtttgt
     8821 tttctaagac cgtcttctat tcgcttcact tcggtatggc taatagcggc aagttctgca
     8881 agccgcctta ttgacagccc tttttccgtt cttttttcct ttatataagt cccgagttcg
     8941 ttcataatat agtccctcct cgcttgaacc tcttttatta taccataagc gtaacctaaa
     9001 agcaacactt ttttgaaaaa cctcttgcat tttgtaacct aaaggtgtat actatagaaa
     9061 acggaacctt aaagttccaa gtcaacctgc cgccataacg cagtatttat tttagaatgg
     9121 tggaatcttt aggttttgga aaggagtatc agatgaagcc aaacatagag tatgtgaaac
     9181 aagagatgga acgccgcaaa tggacaggca gtcagcttgc tatgaaaatg ggtgtttctc
     9241 ggatggaagt gagccgtctt ttgcgtggaa ggcgtgtcgg cggtaagaag tgcatcggag
     9301 gtcttatcaa ggcttttccc gatgcagaac ttaaagacct attttttttg gattaagtgg
     9361 aacttatagt ttccgaagaa aggagaactg tatggaacag gtcaagaatc tcaatgacaa
     9421 gcgtgtttgc gaccagagca aggacggcaa ggtcatcgtt atccgaaaga aagactgtat
     9481 tacacgaatc actgccaatg cagacagaac actcaagatt acgcatgaat atgcgaagta
     9541 aattcaaaca gaaatccgtg agagcgccag acgacagtac ggatacctga atcaaggtat
     9601 cgttctgtcg tttttctttt ttggacgcta aatctgtcct gagcaagaca ttaaactgct
     9661 caccgcccga caccgcatca cctgatcact gatggctcaa cggcatccgg cggcacaact
     9721 caatatcaca gctgcctttt gagcgggtta gctgcaatcc gaaacggaga acttcgttag
     9781 gactgcggtt aggtttttgc accctttttg cggcagcgcc cagagtcctc cgtttcgaga
     9841 aatcgacaaa cggaggactt tttatgaaaa acaatgcaaa tcagaccaca tcaaccatct
     9901 actaccgtcc gctcaagcag tggattgatg tcaccccgga acagaaacgg gactgggagc
     9961 ggttcgtagg gactacccgc aaggcaaagc agagagccgg agcctgctgc atcccgtaca
    10021 aaaagagcta caagtgcgac ggtatttgcg acacctgcga gttccgctgt atcccgaagg
    10081 acgctcccca gcatctctcc atcgacactg aaatggagaa cgcttacgaa aacggcgtct
    10141 cccgcaccag ctttcttgcg gacagcaggc tgaccacgga gatcgatatc gattccctga
    10201 ttctgaacgg cctgctcacg gagcttcagt catccgaccc ggaaagctac gagatcctca
    10261 tggctattgc ggacgggctt tctgaacgcg ccggcgcaga gcggctgcag atgccccgga
    10321 acacatttgt gtataaaaga aatcagctgc tgaagcgact caaggaaaaa ttctaaaatc
    10381 tttcggccaa cccctccttt cctgtccaga tgggtctgtg aaaggcaaca caagacgcct
    10441 tgggaaagga gggaccgctg atatgagtta caacgcaaac cattatgacg cccgtgccga
    10501 cgaggacatt gttgatgtcc tgaccgcaat cagcgtggtg tcaaggagac tggcaagcaa
    10561 cctgaccatc gcacgccagc agagcaaatc cagggaagga ggaaaatcac atgagcagaa
    10621 tgagcgatat ggcacagacc atcgaagact tacgcagtgc tgccgctgct atttcggatg
    10681 ccgctgactg gctgacgaag gtattcagca gtgaaccgca ggcagaggat gctcctgctt
    10741 ctcctcccga accggctctg acgctggagc aggtcagagc cgtgcttgcg gacaaatccc
    10801 gccagggaca caccgccgag attcgcgccc tgcttcaaaa gtacggcgca tccaagctgt
    10861 cacagatcga ccccgcacat tacaaggcat tgctcgccga agcggaggtg ctgactaatg
    10921 gcagttaagc acgcagtctt atccgcttct tcttccgaac ggtggctcaa ctgtccgccc
    10981 tccgcaaggc tgtgcgaagc ctacgaggac aagggcagcg attacgccgc cgaaggaacg
    11041 gacgcccatg cgctctgtga gtttcggctg aagcaggctc tggggattcc ggcggatgat
    11101 cccatcgaaa acctctcctg gtacaacgaa gagatggagg actgcgccgc cgggtatgcc
    11161 gcctatgtat cggagcttct ggagaccgca aagcagacct gtgccgaccc ggtcatcctg
    11221 atcgagcagc gagtggattt ctcccgctgg gtgcaggacg gtttcggcac cgccgactgc
    11281 atcgtcatcg ctgacggtga gctgaacatc gtggactata agcacggcaa aggcgtggag
    11341 gtcagcgccg tagataatcc gcagatgatg ctgtatgccc tcggcgctct ggagatcttt
    11401 gacggtatct acgacatcga ctccgtccgc atgaccatct accagcccag gaaatccaat
    11461 atcagcgtct gcgttatgga aaaggacggt ctgctcgaat gggcgcagaa cgacctgacc
    11521 tataaggcaa agctggcata cgagggcggc ggcgattttc actgcggcga atggtgccgg
    11581 ttctgcaagg caaaggccga atgccgggaa cgagccgaag cgaatctcgc gcttgcccgg
    11641 tacgactttg aggagccgcc cctcctgacc gatgaggaaa tcgccggcat cttggacaag
    11701 gtggacgctc ttaccgcctg ggctgcggat gtgaaggaat acgctcttca gcaggcagtc
    11761 agcggtacgg cgttccccgg ctggaagctg gtcgagggcc gctccaaccg caaatacacc
    11821 agcgaagccg ctgtggccgc agccgttgag ggcgccggct tcaaccccta cgaaaagaaa
    11881 ctcctcggca tcaccgccat gcagaagctg ctgggccaat cccgctttga ggagcttctc
    11941 gcaccctaca ttgaaaagcc gcaaggcagg ccgacgctcg tgcggtcgag tgataaacgg
    12001 cccgaatgga ataccgcaaa aaatgatttt atggaggaaa tgtaatatgt ctaacaacac
    12061 aaacagagtc aacaacccta tgaaggtcat caccggtccc gacacccgct ggtcctacgc
    12121 caatgtctgg gagcccaagt ccatcaacgg cggcacgccg aagtactcgg tatcgctgat
    12181 catcccgaag tccgacacta agacggtggc gaagatcaag gccgccatcg aagccgccta
    12241 ccaggaggga caggccaagc tgaagggcaa cggccgcagc gtgcctcctc tctccgcgat
    12301 caagactccg ttgagagacg gcgacgttga aaggccagac gatcccgcct atgcgaacgc
    12361 ttacttcatc aacgccaact ccgccaccgc tcccggcatc gtggacgcag accgcaatcc
    12421 tgttctgacc cgctccgagg tgtattccgg cgtgtacggc agagcttcca tcaacctgta
    12481 cgctttcaac agcaatggca acaagggcat cgcctgcggt ctgaacaatc tgcagctcat
    12541 ccgtcccggc gaacccctgg gcggcaaggc cagtgccgaa gctgacttcg caactgatga
    12601 cgacgaggat ttcctcggtt aagacaaggg aggtaaacgg ccatgacaat gtttcagaca
    12661 attctgcttt ttgctgttct cgccatctgg ctgtgcatca gcgcagtcat tctgatcagc
    12721 agcatccagt ccttcctcta tgaccgcaag cgcgaaaagc gtgagcgtga acaggcagcc
    12781 cgtgacgcag agtatcatga aaaccgcatg aagctgctgg agaaataaag taccaagccc
    12841 cagggcggcg gagcgatctg ccgccctatt ggggtatgga aggaagtgac gaaatgcaaa
    12901 ccttatcaat cgacctggag acctacagtg accagtccct gcaaaaaaca ggtgtgtatc
    12961 gttatgtgga gtctcccgat tttgaaatac tgctctttgc ctacagcgtg gacggcggtc
    13021 tcgtacagca gatagacctt gcctgcggag agaaaatccc gccagcgatc cttgccgcac
    13081 tggaggacga caaggtgacc aagtgggcct tcaacgctaa ttttgaacgc atctgcctgt
    13141 cacgctttct gggctatccg accggcgact atctagatcc ggattcctgg agatgctcga
    13201 tggtctggac tgcgtacatg ggtctgccct tatccctgga gggagccggc gccgttcttg
    13261 ggctggaaaa acaaaagctg accgaaggca aagacctcat caaatatttc tgccagccct
    13321 gtgcgccaac taagtccaac ggtcagcgca cccgcaatct cccaaaacac gccccggata
    13381 aatggctgac atttaaaaaa tacaacatcc gcgatgtgga gacggagatg tctatccagg
    13441 cacggctctc aaagtatccc gtgccggaca gcgtctggga ggaataccac ctcgatcagg
    13501 agatcaacga ccgcggcgtg ggactggata tggagctggt gcggcaggcc attcagatgg
    13561 acgggcgctc ccgctcggag ctgacccagg caatgaagga actgacctca ctggataatc
    13621 ccaactcggt acagcagatg aagcagtggc ttgcggataa cggcgtggag accgatacct
    13681 tgggcaaaaa ggctgtagcg gagcttttga aaactgcgcc gccgcagctg caaaaggtac
    13741 tgaccctgcg ccagcagctt gcgaaatcca gcgttaaaaa atatcaggcg atggaaaccg
    13801 ccatctgcgc cgatgaccgg gcaagaggta tgttccagtt ttacggagcc aaccgcaccg
    13861 ggcggtgggc aggccgcatc attcagatgc agaatctccc tcagaatcat ctggacgatc
    13921 tgtccgaagc cagagggctt gtccgggcag gcggctttga cgctctggaa atgctctatg
    13981 aggatgtgcc ggataccctt tctcagctga tccgcacggc attcgtgccg caggaaaaca
    14041 ggaaattcat tgtggcggac ttctccgcga ttgaagccag ggtgatcgca tggcttgccg
    14101 gcgagaagtg gcggcaggat gtattcgccg agggcaagga catctactgc gccagcgcgt
    14161 cccagatgtt cggcgtcccc gtagaaaagc acggcgtcaa cggccacctt cggcagaaag
    14221 gaaagatcgc cgaactggct ctcggatacg gcggctccgt gggcgcactt aaggctatgg
    14281 gcgcactgga gatggggctg tcagaggacg aacttccagc tctggtttcc gcatggcgtc
    14341 aggcgaatcc gaagatcgtg cagttctggt gggcggttga ccacgctgtg atggacgctg
    14401 ttacccgtaa gaccaccacg aaaacacacg gcatcatatt ctccgccaga aacgggatgc
    14461 tgtttatcac tctgccgtcc ggcaggagcc ttgcctatgt gaagccaaag atcggagaga
    14521 accgttttgg cggcgactgt atcacctatg aaggcgtcgg cggcacgaag aaatgggaac
    14581 gcattgacag ctacggtcca aaattcgtag aaaacatcgt ccaggcaacc tcacgagata
    14641 tcctttgtta cgccatgaag acccttcgct gctgctccat cgtcatgcat atccacgacg
    14701 aagtggtcat tgaagccgac cgccggatgt cactacaggc cgtctgtgac cagatgggca
    14761 ggactccgcc ctgggcaaac ggactgcagc ttcgtgcaga tggctacgaa actgattttt
    14821 acaagaaaga ttaacgaggt aacgcctatg agcatcaata aattcaacag cgagggctat
    14881 tacgacccca ccacctatga agccctcacc aatatagaaa aggaggaacg cgccctccgc
    14941 gctttccgcc ctatcgtcta catctgctct ccctatgccg gagaggtggc cgccaatgtg
    15001 gagaacgccc ggaggtacag ccgctttgcc gtggacgccg gatacattcc cattgcgccg
    15061 catctgctgt ttccgcagtt cctcaatgac gccgatccaa aggagcgtca gctggggctg
    15121 ttctttggaa acgccctcat gagcaaatgc tccgaggtgt gggtattcgg agaatacatc
    15181 tcttccggca tggaagcgga gatccgcaga gccaaatgga agaactaccg tttgagatat
    15241 tttacgacta tgtgcgagga ggtaaccgac tatgcgtgaa ctgaacatcg cctacggcaa
    15301 caaccggcag gcaaagagat gggtcaacaa gaccatacga tttgacaatc taaaggagcg
    15361 gctcaaagtg accatccgca ccaccgagtc cgccgaggaa tatgcgaaga tgagccgcgc
    15421 ccagcgtgac gccgccaaag accacggcgg ctttgtggcg ggcgtgctga agggcggcag
    15481 acgcaaggtc gataccgtgg aaagccgctc gatggtggcg cttgacggcg accgcatcga
    15541 caccgctttt ctggatagct atgagtccct ctgcccctat gcctctgcgc tgtacaccac
    15601 tcacagcagc acagaggaga atccccgcgt ccggctggtg ttcccgctga ccagggatgt
    15661 gaccccggag gaatttgtgg cggtgtcccg ctatctggct cagatgctgg gcatcgacta
    15721 tttcgacgaa tgttcctacc agcccaatca gctgatgtac tggccgtcca ccccggctaa
    15781 cggctccttt gtgtataagg agacagacgg tggctggctc gaccccgatg cgattctcac
    15841 aaagcacccg gaatggaccg accctacaag actgcccaca tcttccaggg aaagcaaggc
    15901 gaataccacc gcacagcaga aggtgcagga cccgctgacc aaagagggcg tggtgggtct
    15961 gttcaaccgc acttattatc ccatcagcaa ggcgctggag acatttctct ccgatgtcta
    16021 tgagccgacc gacaatgaga accgctggca cctgatcgca tcctccagta tggcgggcgt
    16081 ggagattaag gaagataaat tcgtctacag ccaccatgcc aaagatcctg cttacctcaa
    16141 gctgtgcaac gcctttgaca tcgtccgtat ccaccgcttt ggcgatctgg acgaaaaagc
    16201 gtcctataag gcgatgtgcg agttcgccat gcagcaggat gaggtaaagc tgctggcggc
    16261 aagcgaaaga atggcggacg ccgagacgga tttctccggc agcgaggata ccgactggca
    16321 gaagcgtttc cagtacgaac cccgctccac ggtgctaaag aacaacctcc acaacatcac
    16381 gctcatcctc cagaacgatc cgcagctcca gaatatcgtg ttcaaccagc agctggacgg
    16441 catggagatc aagggcgatg tgccctggaa gcacccgtcc aagtattggc gggacgccga
    16501 tgacgcacag ctgatcagct atgtggactc ccattacggc acattctccc agcgcaacta
    16561 tcagatcgcc gtgaccaagg tggcagacga ccgctcctac catcccatcc gcgaatatct
    16621 ggcggctctg ccggaatggg acggcgtttc ccgtgtggac acgctcctca tcgactatct
    16681 gggtgcggag gataattcct acgtccgtgc cgtgaccaga aagaccctct gcgccgccgt
    16741 gcgccgggtg caggagccgg gtgtgaagtt cgataccatg ctggtcttaa acggtcccca
    16801 gggcatcggc aagtctaccc tcatctcccg ccttgccgga gagtggttct ccgacagtct
    16861 gaacctgagc gacaccaagg acaagaccgc tgccgaaaag ctgcagggct attggattct
    16921 ggaaatcggt gagctggcag ggcttcgcaa agctgaggtg gagacgctgc gctccttcct
    16981 ttcccgacag aacgatatct accgcgccgc attcggcaga cgggcaacgc cgcacccaag
    17041 gcagtgcatc ttctttggca ccaccaatgc cgagtccggc tatctgcggg ataccacggg
    17101 caaccgccgg ttctggcctg tcaaaacgcc gggcggcggc ataaagcact cctgggaact
    17161 taccaccgag gatatcagcc agatctgggc ggaggtgctg gtgcttgtaa aggacggcga
    17221 gaagctgcat cttgctcccg acctggagat gctggcgaaa agcgaacagc gggaagccct
    17281 ggagtccgat gagcgtgaag ggctggtgcg cgagtatctg gagaccctgc tcccggagga
    17341 ttgggacggc atggatctgt tcgaccgccg ctccttcctc gccggagtga acaatattgg
    17401 ccgtgtgggt acggtcgcca gaacacgggt ctgcaatatg gagatctggt gtgagctttt
    17461 cgggaaggat cagggcagcc ttggccgcgc cgaatccaac aatctcacgg ccatgctcac
    17521 gaaactcggc tgggtgcgca aggagaaaaa ggagcgcgtc aagccctacg gaccgcagtt
    17581 cgtctttgtt cccggcgatg ttcctgactg atttttcggg aacggagcga ataaggaaca
    17641 gttcccgact tccggcagtg ttcccagggg agactccggg aacgccgcca ggaacacacc
    17701 gaatgtgccg ccgcaaggca actttacagg cttcgttcct gtgttcctaa aaaagtatac
    17761 aaattgaaaa tgtataaaaa agactgtaca gaacccgtaa aacacgcata cgcacgcgcg
    17821 taaggatttt caggttttta agaacacgga ggtaaatcaa aatgtcaatg tatgaaatag
    17881 acagcgcata tgtccgcagg tgtcagaagc ggcttcggga atggggagcg cctctctccg
    17941 gctggtactg tgactacatt tacgatgtgg ccgatgaaga ggaagatccc gatcacatcg
    18001 acctgttcac ctgtgaactc tgcgattgtt cacaggtacg ctttgtccat gtgatgcgtc
    18061 atgacgagta ttttgagact gtttcagtcg gctgtatctg cgcagggatt atggagggcg
    18121 atatcttcgc cgccagagag cgtgagcggc ttatgaaaaa ccgcgccaag cggaaacgta
    18181 actttcccca ccgtcaatgg cgaaagaact ggtatggaaa ctatcagctg acttatcggg
    18241 gcagaaaggt gtttatcaac aacaagggca gcaatcgcta cagcgtatat gtggatggca
    18301 ggaccgcctg gagctacaag ggcaagcccc tcgacaattt tgtcttggcc gcctacgccg
    18361 cttttgaatt agccgacccc atagaaagga tacgcccatg agagaaaaag aaattgaaaa
    18421 gaaactgacg cagatggtca aaaaggccgg cggcatctgc cccaagctcg tatctcccgg
    18481 ttttgacggt atgccggacc gcatggtgct gctgccgaat ggacggatag gctttgttga
    18541 agtcaaggct ctgggagaaa agccgcgacc tctgcagctt tcccgccaca ggcttttgcg
    18601 gcggctggga tttccggtgt atgtgctgga cgatacggag cagattggag ggatactgga
    18661 tgagatacga acctcatgaa tatcagaaat atgcggtgga ttacattgag acacaccccg
    18721 cagccgctat cttcttagac tgcggcctgg gaaaaaccag catcacgctg acagccatag
    18781 ccgacctgct gtttgacagc ttcgaggtcc ataaagtgct ggtcatcgca cccttaagag
    18841 tggcgcggga cacatggacg gctgaagcgg ataagtggga tcaccttcag gatctcatct
    18901 gctccgtggc tgtcgggacg gaagcccagc gccggtcggc attgatgaga cgcgctgaca
    18961 tctacatcat caaccgggaa aacgtccagt ggctcattga tgagagcggc attccctttg
    19021 acttcgatat ggtggtgatc gatgagttat cctcgttcaa gaatcaccag acaaagcggt
    19081 tcaagtcact gttaaaggtc agaccaaagg tgagccgcat cgtcggactg accggcactc
    19141 ctgcgtccaa cggtctgatg gatctgtggg cggagtttcg catcctggac atggggcagc
    19201 ggcttggacg gttcatcacc aagtaccgca ccgattactt cacgccggac aagcgaaacg
    19261 gccaggtcat ctactcctac aaacccctgc cctatgcaga ggacgccatc taccgacaga
    19321 tttcggacat caccatctct atgaagtccg ccgaccacct gaatatgcca aagctggtca
    19381 gcagtgaata cacggttcgg ctttccgagg atgaacagaa aaaatacacg gacttgaaac
    19441 aggagctggt gctgtcgctg ggcaacgcag agatcactgc cgccaacgcc gcctccctct
    19501 ccgggaaact ctcccagatg gcgaacggcg caatctatga cgacggcggc gaaaccatcc
    19561 gcatccacga ccgcaagctt gacgctttgg aggacatcat cgaagccgcc aacggcaaac
    19621 cgcttctggt ggcttactgg ttcaagcatg acctgacccg tatttcggaa aggctgcgaa
    19681 agctgcatat cccgttctcc cggctggatg acgccgccag tatccgcaga tggaacaatg
    19741 gcgagttacc ggtggcgctg atccatcccg cttccgccgg gcatgggctg aatctccaga
    19801 gcggcggttc ggccatcgtg tggttcggac tgacctggag cctggagctt tatcagcaga
    19861 ccattgcgcg gctctggcgg caggggcaga cttctgaaac tgtggtggta caacacatcg
    19921 tcacaaaggg caccattgac gaacgcatca tgaaagccct ctccaaaaag gagcataccc
    19981 agacggccct gatcgatgcc gtgaaagcgg acttgaaaat ctgagacaat ctaagaaaat
    20041 ccgtgccaat ccgaggatca aaatttcgga ggtgcgaata tgagcaatat cacgatttac
    20101 gaggaccttg cgaacgccat catcctgcaa gctgtgaagg attaccgcat ggcgctgaaa
    20161 agcctgaaag ccaactcccg taatagggcg gcgcaagcgg ataaagccga aattgagcgg
    20221 ttcttccgtt cgcagtggta ttcggcactc acagatgtga aaggcgagat gctgatccgc
    20281 tccctgcaga aggaggtgga cgcatgaccg caaaagagta tctgaaccag gcgcggcacc
    20341 tggatgccct tataaactgc cgtctgcgtg agattgacta ctggagggat ttatcgagca
    20401 gcgtctcagg cagtaatttc gaaccgcact acaacccaaa caagccgaca gaagcccctt
    20461 ttgtccggtg ccttgagaaa atcgacgcca tccagaggga tgtggcggaa aaggtggcgt
    20521 atctggtgtg tctcaaggaa accatcaatg cggcaatcga caggcttgcc agccgtgagg
    20581 agcagctggt actccgttac cgttacctgg acaactgctc ctgggaggag atatcccgga
    20641 tgctgaacgt gtcgcttcgc acggtacacc gcatacacgg gtcggctctc cagaactttt
    20701 ctgtcccgaa ttgaaagttg gcacggtttg gcacagtatg gcacacccgt cctgtgatat
    20761 cattacaata gcgaagtaga atacaagaac agccttcatg ggagcgatcc tgtgggggct
    20821 tttattatgc cccaaggagg tgaagcgatg ccaaggaaac ccaagcggcc ctgctcctac
    20881 cccggctgtc ccaacctcac ggatggtcaa tactgcaagg agcacgaagc agccgcccgc
    20941 aggcagtaca acaaatacga acgcagtgcg gacgtaaaca agaagtacgg aagagcctgg
    21001 aaacgaatcc gtgaccgcta tgctgcggcg catcctctct gtgagatgtg tctcaaggaa
    21061 ggacggctga ctcctgtgga tgaggtacac cacatcgttc ccatctctca gggtggcact
    21121 catgcgagag acaatctgat gagcctttgc cgttcctgcc ataccaagat ccaccacgac
    21181 cttggcgacc ggtagggcgg tcaaaatctc tgcgggtcct gtacgcgggc agcggcccgg
    21241 ggcttcgtgc gcgaaaaagg cgaaatcaaa agggtaattg acggcggccg atagcggtcg
    21301 ctctattttt gcggaaaaga ggtgagaaaa tgccgacaaa atccaataac acaggtgggc
    21361 gcggtggtgc gagacccggt gcgggaagaa aaaagtctgc cgtcaaggag aaagccgaga
    21421 acggcaatcc gggcggacgc agactggaag ttctggacat tcccgaagtc gagggtgtcg
    21481 atatgccaaa gccccatgag ttcctctccg ccgagcagcg tgacggaagt acgctccagg
    21541 cggaggaaat ctacaaagaa acctgggagt ggctgaagaa gatcggctgc gccgcaaagg
    21601 tatcgccaca gcttttggag cggtatgcca tgtgcagcgc ccgctggatt cagtgcgagg
    21661 agatgaccaa ccgcatggga ttcctctcca agcaccccac tacccagaag ccgatcccat
    21721 cgccgtttat caatatcggc atcaattaca tgaaccaggc ggtgcggctg tggaatgaaa
    21781 tcttccagat cgtcaaggaa aactgcagca ccgactacgg ggaggtctct ccccaggatg
    21841 atttgatgga gcgcttactg cgggcgcgga aaggataacg ccatgtttga aaaagtaaat
    21901 ccgtgccacc cggacaaggt ggctgaccgc atcgccggcg ctctggtgga tgcggcgtac
    21961 agaaaagatg aaaatccgag gatcgccgtg gaggttctca tcggccacgg tgtctgccac
    22021 atcatcgcgg agagttccgt acacattccg ctggatgagg tgtatgccat cgtgaagcgc
    22081 atcggcggta atctgcacac ggattatgtg gaagtgccgc aggacggaca cctcgccaat
    22141 aaccaggcgg acggaatccg ctgcggcgac aacggtatct tcaaagggat gcctgtgacc
    22201 gaggagcaga aagccctctg cgagatcgcc agaagtgtgt atcacactta tcactatgac
    22261 ggcaagtaca ttctggacgg cgcaaggctc atcctctgcc agagcaatgc caggactcag
    22321 cacctcaagg aagtatatcc tcacgccgag gtaaatcccc tgggtgactg gacaggcggc
    22381 acggatgtag actccggcgc caccaatcgc aagctgggca gtgatatggc tgactccgtc
    22441 acaggcggcg gtctgcacgg aaaagaccta tccaaagcgg atgtgtcggt caacatctac
    22501 gcctggctga aagcgcagaa aacgggaaag cccgtggaac tgtgctgcgc catcggggac
    22561 gataccgtgg acagcattcc ctacgctgag atcgtggaaa ccgcccggag atacatcctg
    22621 tcccttggcg gctttgagaa atttgcggaa tgggggctgg tacgatgaaa acgacaacgg
    22681 aaatgcagct cgtgcctatt gccaagctgg taccctatgt aaacaacgcc cgaacccact
    22741 ccccggagca gatcacgaag ctccgctcgt ccctccggga gttcggcttt attaatcccg
    22801 tcatcatcga ccgtgacttt ggcattatcg ctggtcacgg tcgtattctt gctgccaaag
    22861 aagaaggcat caaggaagtt ccgtgcgtct ttgctgacca tctcaccgag gcgcagaaga
    22921 aagcctatat cattgcggac aaccgcatgg cgatggatgc cggatgggat gaggagcttc
    22981 tgcgggtgga gatcgagtct ttgcagggca tggactttga ccctctgctc accggctttg
    23041 acgaaaagga gctggcggct ctgttcgatg acggcatcga agctaaagag gatgacttcg
    23101 atgtggatgc ggagctgcaa aagcctacct tcaccaaaag cggcgatgta tggacgcttg
    23161 gcaggcatcg gctggtatgc ggggactcca ccaaaccgga ggtctatgac atcctcatgg
    23221 acgggactaa ggcaaacctg gtgatcaccg acccgccgta caacgtcaat tatgagggtt
    23281 cggcgggcaa aatcaagaac gacaacatgg caggcgagaa attctatgag tttctcctcg
    23341 ccgcttttca gaatatggag tcggtcatgg cggcggacgc atccatctat gtgttccacg
    23401 ccgacaccga gggcttaaac ttccgcaggg cgtttgccga tgcgggattt tatttatccg
    23461 gctgctgtat ctggaagaag cagtccctgg tcctcggacg ctctccctac cagtggcagc
    23521 atgaacctgt gctgtacggc tggaagaaaa acggcaagca ccaatggtac acgggcagga
    23581 aagaaaccac catctgggag tttgataagc ccaagaagaa cggcgaccat cccaccatga
    23641 agccaatccc cctgctggca tatcccatcg gcaactccag catggcaaac tcggtagtgc
    23701 ttgacccctt cggcggctcc ggttctaccc ttattgcctg tgagcagacc gaccgtatct
    23761 gccgaaccat tgagctggat gagaagttct gcgatgttat cgtgaaccgc tacattgagc
    23821 aggtcggttc tgcggatggc gtgagcgtcc ttcgggacgg caggacatac agctatgagg
    23881 aggtcacgga tggaacacaa taaactgacc ctcggcagcc tgtttgacgg ctccggcggt
    23941 ttccctttag gcggcttgat ctccggcatt acccctatat gggcttcgga gatcgagccg
    24001 tttcctatcc gggtgaccac caaacggctg ccgtttatga agcattacgg cgatgtctcg
    24061 aaaatgaacg gcgcagatgt agagccggtg gatatcatca ccttcggctc accatgccag
    24121 gacatgagca tcgcaggccg acgggagggc ttggacggct cccgttccag tcttttctac
    24181 gaagccgtcc gaatcgtaaa ggaaatgagg tgcaaaacca atggcagata tccaagatac
    24241 atcgtctggg agaacgtccc cggcgcattc agctccaaca agggcgcgga cttccagtcc
    24301 gtcctcgaag aggtctgctc ggtcaaggga tacgaaattc atactcctcg acctgagagg
    24361 tggccgaccg ccggagagat cgtggcagat gatttcagtc tcgcatggcg gatatttgat
    24421 gcgcagtact ggggagttcc ccagcgcaga aaacgtatct accttgtcgc agatcttgca
    24481 ggcgggagtg ccggaaaaat actatttgag tccgaaggcg tgtctgggta tactccgcag
    24541 ggcttccgtt cgtggcaagg aactgccgga acttctgaaa aaggctctgg agcggcaggc
    24601 tgcgtctgcc tgaacgacca gggtggaaac cgtatggatg tgacggacgg cgtcacctgc
    24661 actttgcggg cagaagttca tcatccaccc tgcgtgatgg aatcggcagg cttttgcacc
    24721 gagcattctg cacacagccg ctccatcggt tatgaggagg aaacctctcc caccctccgg
    24781 gcggggacgg ttcctgcggc agtctatgag aaccatagtc aggacaccag atacaccaaa
    24841 cttgagggaa ttgctcccac cgtttcatca acctacggca caggcggcaa taatcagccc
    24901 ttcgtggtgg aaacgccaaa gacgctgaag atccgctccg gctgtgaggg cggcggcaaa
    24961 ggtgcattaa ttcaggacaa caagtccgct accctcggct gcaacaatga ccagacagta
    25021 tttgtgccga aggtctacgg catctgctcc aaacagagcc atgccatgca gtcggacaac
    25081 ccacacagcg gtttttatga agcggatact tgccgctgtc tggacgcaaa cggcggtaac
    25141 ccttcctaca accagggcgg catggctgtg gtggctgtgc aaggctccat gatcggaagg
    25201 tccgacaaga acggtcccca gggcagcggc gtcaatgagg atgtgtcctt cacgctggat
    25261 gctaccgacc gccatgcggt agcctacggc attgaccggg ccacatacaa tatgggacgg
    25321 aacgctaagt tcggaatcac ggtggaaacg gagatcgagc caacgatggt gtccaaggga
    25381 ccgggagcgg tcggacagcc tatctaccat tcaagcaaga gttccttcca caccaatttt
    25441 acggatgatg atgtgacgga aacgctggtc gctacagatt tcaaagatcc gcctaccgta
    25501 tccaaggagc ctgactacat cgtccgcaga ctgacgccta ccgaatgcgc cagactgcag
    25561 ggattcccgg actggtggtg ttccgacctt ggcacggcca atccgaccga ggatgaagta
    25621 gagttctgga cagaggtatt tgagacacac cgaaaggtca tgggtgcatc ctccaatcca
    25681 aagagccgaa atcagatcat caagtggctg aaagaccctc actccgattc tgcggaatac
    25741 aaaatgtggg gcaacggagt ggcgctgcct aatgtctgtt ttgtgctttc cggcatcgtg
    25801 tactatgcac agttatccgg cgaatgatcc ggcagctatt ctacagcaaa atgtgcggaa
    25861 atcgcttgct atttcagggg ttcagagcga atatgtgact accaaaaaca aaggaggttt
    25921 tcgcacatgg aaatcagata caatgtgaca ggcgcaaagc gcaaggaact ggtcaaggtc
    25981 atttcggatg ccacgggcgc cagggcggaa tacaaattca tgcccacctg caattatgag
    26041 atcgactact tcacggtcac caaggacggg acgctcctgt ttgacgaccg tgctgacagc
    26101 gaggaggtcg agcaggtact tgaagctatc accgccgcag gctttgaatg cgaggcgaag
    26161 ggcggcgagg aacagttctt cgaggaagaa tccgaagcca ccgacactgc gccacagagc
    26221 gaaactgtgg ggcttacagt ggagatcccg ctggacaaag cggcggttgg caaccttact
    26281 aaactgctgg atgccaaggg tagcctgatt aagaaggcgc tcggtgttag cgaacttcca
    26341 attgagattc aggaagaccg ggtaacgttt ccctggttcc cggaactgcc ggacgccgat
    26401 gcagttaaag cctacaccca tttcatttcc gcactctgcg agatgagcaa aaacgccaag
    26461 cgcgtgacgg tcacggagaa agcggtggat aatgagaagt acgccttccg ctgctttctc
    26521 ctgcgcctgg gcttcatcgg cagcgaatac aaagtcgaac gcaagatcct gctgaagaat
    26581 ctctccggtt cttcggcatt taagaacgga ggtgtagacc atgcgatttc cgagtaagga
    26641 agttgtagaa cgtatccgca aggagttccc atcaggcacc cgagtggagc tgctgcggat
    26701 ggacgatccc caggcaccgc caatcggcac acagggcact gtgatgggcg tggatgatat
    26761 cggctccatc atggtcgcct gggacaacag cagcggcctt tccgtgtcgt tcggagaaga
    26821 cctgtgccga aaggtcaaga acacatctga tggtagtaaa aaggtataaa ctacacaata
    26881 tcttgcggtc atctttgtgt agtatattat cgaaaatggc cttgctatta tcctctttta
    26941 gagcgaatat gtgtacaccg aaagggaaaa cacaccaaac gaaaacggag gattcaccat
    27001 gaacgagaaa acagcaaggc agatcgcaga gatgaaaaac cagaccatcg gggtcgaggt
    27061 cgagatgaac agcatcaccc gccagaaggc agccaaggtt gccgccgact tcttcggcac
    27121 aggcagatac gagaataccg caggccgcaa cggctacagc acctggagcg cctgggacgc
    27181 acaggggcgc gagtggaaat tccagaagga cgtttccatc gcaggagcgg acgagcagaa
    27241 atgcgaactg gtcactccga tcctgaccta cggggatatc gaaaccctgc aggagctttg
    27301 cagacagctc agacacgcag gagcgaaaag cgacgcctcc agaggatgcg gagtccacat
    27361 ccacatcggg gcgcatggcc acacaccgca gagccttcgg aacctcgcca acatcatggc
    27421 aagccacgaa agcctgattg ccgaagcctt aaagctcgac cgcagccgca tgagccgcta
    27481 ctgccgcacg gtagacccca acttcctcgc caaggtcaac agcagaaagc ccaagacgat
    27541 ggcgcagctt gcggacatct ggtacaccag ccacggcgca agctacggca ggaaccagca
    27601 ttacaacgac agccgctacc atatgctcaa cctccacgcc acctttacca aaggaacagt
    27661 cgagttcagg ctcttccagt ttgacgagcc gaccgcagag cgccggggcg gcatccacgc
    27721 aggacagctc aaaagctaca ttcagctttg cctggcgctg agccagatgg caaaggatgt
    27781 gcggacggca agccccaaac cccagcagaa cgagaacccc aaatacgcca tgagaacctg
    27841 gctcctccgc ttgggcttca tcggcgagga gttcgcaacg gccagagatt tcctgacccg
    27901 caaccttact ggagatacag ccttccggca cggcagagca gccgcttgaa ggatttaggt
    27961 taaatgccct gcccctgacc gcttcggcgg tcttagggtg gtagaaggac aagtaaccta
    28021 agtcctccag gaaaggatgg atacacatga aagaaaaaag atactacatc gcctacggca
    28081 gcaacctcaa tgttccgcag atgcggatgc gctgccctca cgccacgatc ctcggcacgg
    28141 ctaaccttaa aggttgggag ctgctgttca aaggaagcaa gaccggctct tacctcacga
    28201 ttgaggaatg cgaaagcggc acggttcccg tggtgatctg ggaggtgacg gctgcggatg
    28261 aagccgccct cgaccgctac gagggatttc ccaatttcta ctacaagaag gacatcaatc
    28321 tccaatacaa gggaatccgt acagggaaac gcagaacggt gacggccttt gcctacatca
    28381 tgcatgagga gcgccccatc ggcatcccca ccaactttta catgaggacc tgcctggagg
    28441 ggtacgacac cttttacttc gacaagaaca ttctgatcga cgcctacgaa aaatgcaggg
    28501 aggtatgcgg ctatgaagga taacatcata aggacggcgg tctgcccgct ttgcggcagg
    28561 acttatcatg gcgctccggc gctttccagg gaggacaaca agaccctcat ctgcccagac
    28621 tgtgggacaa ggcaggcgct ccggtcaatc ggcgtggaca ccgccgagca ggaacagatc
    28681 atcgagacga tccaccgcca tatgcaggag taatgtacac agtttcctcc actgatcttt
    28741 gtgcagattg tgctcggaat taacttgcta ttctgtgctt ttagagcgaa tatgtacaca
    28801 ccgaaaggga aaacaaagaa aaacggagga aaccaccatg atgaacattt accagatgag
    28861 gaacagcttc agccttaagg agcataacac cgcaatcacc agagaggatt ttgaagggag
    28921 ctttaccagg acccgcgaga gcgtccgctt caccttcaac ggctgggatg gcaagagcta
    28981 cgacggtgag agccgcagcg caaaggtcta ccgcaccagc cttcccggat acgagaacac
    29041 acgatttgtg aaggtcggaa aagccctttg ctacatcgat gaagacagca gcattctgga
    29101 gaaagccacc ggcgaatacc aaaaggaagc ggaatggctg gtggatgttc tcaggagcaa
    29161 ctaagaaatc ggaggatacg atgatggaaa acacaggaat taagagatac gaagactaca
    29221 ccgagaattg cgaaaaccac taccggctac cgaacaccag cacgatggag aacctttcga
    29281 tgaaggttac cgccggagaa ggcgcagtgc tgaagatggg cgataaggta ctggtcaccg
    29341 accacgcatg gaagggattc atcgccggag tgtacgagtt cattgagacc ccggaggaaa
    29401 ccggatacag ccacatcgag tgccgactga acctcatcgc catgagcggg gaacttttcg
    29461 aggatggcgg tcacgctatt gcctgggcga tgcagcagta aataacagcg aaaacaatac
    29521 cctgagaacg agccgcacgg ctctttctct cgtacagata cattttggaa gtcgcagtca
    29581 tgcggctctt ttttatgcca ttttggaggt ggtgcctatg cgaaaactga agaaatacaa
    29641 gcctaccagg tttatggcga aaacctcgca ctacgataag gacgccgccg actatgcggt
    29701 catgttcatc gagtccctct gccacaccaa aggcacctgg gccggtaagc cctttgaact
    29761 gatcgactgg caggagcaga tcatccggga cctgtttgga gtcttaaagc ccaacggcta
    29821 tcggcagttc aatacggcat acatcgagat accgaagaaa cagggcaagt cggagcttgc
    29881 cgccgctgtg gcgctcctgc tcctgtgcgg ggacggtgag gaacgcgccg aggtgtacgg
    29941 atgcgccgcc gacaggaacc aggcaaagat cgtctttgat gtggcggtgg atatggtgcg
    30001 gttctgcccg gcgctttcca agcgcgtaaa gatcctggaa tcccagaaga agatcaccta
    30061 tctgcctacc aactcatcct atcaggtact gtcggcagac gtggcaaaca agcacggctt
    30121 caacacccac ggcgtgattt tcgatgagct gcatacccag cccaaccgaa agctctttga
    30181 cgttatgctc cagggctccg gggacgccag gatgcagccg ctgtatttcc tgatcactac
    30241 tgccgggaac gacaccaact ccatctgcta tgaggtgcat cagaaagcca tcgacattgc
    30301 ggaaggcagg aaggtcgatt ccaccttcta ctccgtcatt tacggtgctg ccgaggacga
    30361 ggactggaca gacccggagg tgtggaagaa agccaatccc tctcttggca tcacggttgg
    30421 catcgacaag gtgcaggccg cctgtgaatc cgcccagcag aatcccggcg aggagaacgc
    30481 cttccggcag ctaaggctca accagtgggt caagcagtcg gtgcgctgga tgccgatgga
    30541 gaaatgggat gcctgtgcat tccctgtttc cgaggacgat ctggaggggc gcatctgcta
    30601 tggcggtctg gatctgtcct ccaccacgga tatcacagcc tttgtgctgg tgtttcctcc
    30661 attggatgag gaggataaat actacatttt gccatacttc tggataccgg aggaaacact
    30721 tgaccttcgt gtccgcaggg atcatgttcc ctacgacctg tgggagcgcc agggcgtgtt
    30781 gatgaccacc gagggaaatg tggtccacta cggctacatt gaaaaattca ttgagcagct
    30841 gggcgaacgg ttcaatatcc gggagattgc tttcgaccgc tggggcgctg tgcagatggt
    30901 gcagaacctg gagggcatgg gctttacagt agtccccttt gggcagggct ttaaggatat
    30961 gtccccgccc accaaagaac tgatgaagct ggtactggag gagaaaatcg cccacggcgg
    31021 ccacccggtg ctgaggtgga tgatggataa catctacatc cgcaccgacc cggcaggcaa
    31081 catcaaggcg gacaaggaaa aatccacaga gaagatcgac ggggccattg ccaccatcat
    31141 ggggcttgac cgggcgatcc gctgtggcaa cgatacggga gcttcggttt atgacagccg
    31201 gggccttttg tttatttgag gaaggacggt gatcatatat gggtatcttt tcagggcttt
    31261 tccgttccag ggataagccc cagaaccgca ccacgggcag cacctacagc tttttctttg
    31321 gcagcagctc ggcaggcaag cgagtgaatg aacgctccgc catgcagatg acggcggtgt
    31381 attcctgcgt ccgtatcctg gcggaggctg tggcgggact gccgctccac ctctaccgct
    31441 ataaggagga tggcggcaag gaaaaggcca tctaccatcc gctgtatctg cttttgcacg
    31501 atgagccgaa cccggagatg agttccttcg tgtttaggga aacgctcatg acccaccttc
    31561 tcctgtgggg aaacgcctac gcccagatca tccgcaacgg caagggcgag gtcattgccc
    31621 tctacccact gatgccggac agaatgacgg tggaccgtga cagggacggc aagctctact
    31681 acgaatacac cgtcagcacc gacgatgcgc caactgtcaa gggtacagtc gtgcggctga
    31741 aaccctcgga tgtgctgcat attccggggc ttggctttga cggacttgtg ggctacagcc
    31801 ccatcgctat ggcaaagaac gccatcggca tggcaatcgc ctgtgaggag tacggggcga
    31861 agttcttcgc caatggtgca gctcccggcg gtgtcctgga gcatccggga accatcaaag
    31921 accccggacg tgtccgggaa agctggcagt ccaccttcgg cggcagcggc aacgccaaca
    31981 agatcgccgt tttggaggag ggcatgaaat acacgcctat cggcatctcc ccggaacagg
    32041 cacagtttct ggaaacgaga aagttccaga tcaacgagat cgcccgtatt ttccgggtgc
    32101 cgccccatat ggtgggcgac ctggagaaat cgagcttttc taatattgag cagcagtcgc
    32161 tggagttcgt gaaatacacg ctggagccct ggctggtgcg ctgggagcag tccatccagc
    32221 ggacgctctt ttccgcagag gaaaagaaac agtattttac caagttcaat gtggaggggc
    32281 tgcttcgcgg cgactacgcc agccgcatga acggctatgc cacggcaagg cagaacggct
    32341 ggatgagcgc caacgacatc cgggaactgg agaatatgga tcgtatccct gccgaggatg
    32401 gcggcgatct ctaccttatt aacggcaata tgctcccgct gggtaacgcc ggagcttttg
    32461 cagatacaca accgaacgat gacggaaagg aggaaaatcc cgatgaagaa gttctggaag
    32521 tggacaaaca ggacggtgac gaatcaggag aatcagacgg aaacagtgga gagaacgctg
    32581 ttcctgaacg gcaccatcgc cgaggaaagc tggtttgacg acgacatcac gccgcagctt
    32641 ttcaaagagg aactgatggc aggctccggc gacatcaccg tctggatcaa cagccccggc
    32701 ggcgactgtg tggcggcggc acaaatctac aatatgctga tggattacaa gggcaatgtc
    32761 acggtcaaaa tcgatggcat tgcggccagt gccgcatcgg tcattgcgat ggcaggcaca
    32821 aaggtgctgg tcagccctgt ctctatgatg atgatccata accccatgac cgtggcgatg
    32881 ggtgataccg ctgaaatgca gaaagccatc gagatgctct caagcgtcaa ggattccatc
    32941 atcaatgcat atgagatcaa gaccggtctc tcccgcacaa ggctctccca ccttatggac
    33001 gcagagacct ggatggacgc aaacaaggcg gtggagcttg gctttgctga tgaagtcctg
    33061 aaacgctccg aagtgccgga ggatatggag ccgcctgcgg tgtccatgct gtattccaag
    33121 gctgctgtgg ttaattccct tatggataag atcgcagcga aatgtaagac caaccctaag
    33181 aaaaccgaag accccaaacc ccagggccgc tccgtagaca gtctctatga gcggcttaat
    33241 ttgatgaaat tttaaggagg ataacgacta tgactattct tgaactgcgc gaaaagcgcg
    33301 ccaaggcctg ggaggccacc aaggcattcc tggattccca cagaaacgaa aaaggtatgc
    33361 tgtctgctga ggacgatgcc acctactccc gcatggagca ggagatcacc gatcttggca
    33421 aggagatcgc ccgtctggag cgccaggagg cgctggaggc ggaactgaac cgcccggtaa
    33481 acaatcccct caccggcaag cccatgaacg gcaaggaaac tgccaagacc ggccgcgcca
    33541 ccgatgagta ccgccagaac ttctggaaca tgatgcgctc caagaccccg atgcccactg
    33601 tgatgaacgc tttgcagatc ggcaccgatt ccgagggcgg gtatctggtt cccgatgagt
    33661 atgagcgcac tctggtagaa gctctggagg aggagaacat cttccgtcag ctggcgaaga
    33721 tcatccagac ctccagtggc gaccgcaaga tccccgtggt ggctaccaag ggtactgcat
    33781 cctggatcga tgaggaaggc gcatatctgg agagcgacga ctccttcggt caggtgtcta
    33841 tcggcgctta caagctgggc accatgatca aggtatccga ggagcttttg aacgacagcg
    33901 tctttgacct ggagagctac atctcccgtg agtttgcccg ccgtatcggc gccaaggagg
    33961 aggaagcgtt ctttaccggg gacggctccg gcaagccgct gggcatcctt gccgccacgg
    34021 gtggtgcgga gaccggcgtt accgccgcgt ccgcgaccgc tatcactgcc gatgagctga
    34081 tcgacctgtt ctattccctg aaagcccctt accgcagaaa cgctgtgtgg gtgctgaatg
    34141 attccaccat caaggccatc cgcaaactga aggacaacca ggggcagtat ctctggcagc
    34201 cttccctgac cgcaggtgcg cctgacctgc tgcttggaaa gcctgtacgc acctctgcct
    34261 atatgcctgc cattgccgca gacgccaaga ccatcgcctt cggtgatttc agctattact
    34321 ggatcgctga ccgccagggc cgttccttca agcgtctgaa cgagctgtat gccgctaccg
    34381 gccaggtggg cttccttacc tcccagcgtg tggacggcaa gctgatcctg ccggaagcca
    34441 tcaaggtgct ggcgcagaag tctgcgggct aatgatgaag ggaggcggcg gtgatggaag
    34501 cacttctttc taaggtcaaa gccaatctca ttctggagca ttcggcggat gatgagcttc
    34561 tgaagggcta catcaccgcc gctgtttcct atgcggaaag ctaccagcat atcccggcga
    34621 gccactatac tgacaacgca atgccggcca ctaccgaaca ggcggtgatc atgctggcat
    34681 cgcatttcta tgagtccagg gatggctcca caggcggctt ctttgcggat aacaccaacg
    34741 cggcgcagca ggtgtggaat acagtgaatc ttctcttacg gcttgaccgg gattggaagg
    34801 tgtgagtatg agctttggca agatgaacac cttcctttcc atcgtagaga aacagtttac
    34861 acaggatgag gacggcttca agacggaaac agatgtgacc gtggcagagg ttctggcata
    34921 ccgggaaggc cgtcatggca gtgagaaatg ggcgaacatg gcgtccttct ctacggccac
    34981 cgatctgttc cgattccgtg taatcccagg tgtggaaata acaacggata tgcgaatcct
    35041 ctgcaacggg catacctttg agatcacttc tgtagaggat gtcaaaggca gaggaatgta
    35101 tctggaggta ctggggacgg aggtgaaagc gggtggctaa agcaacatgg aaaatgccgg
    35161 aggactttct gatgaaggta tcccggctgg cggataagac ggatgagatc atcccgaaag
    35221 tgctggaggc gggtgcagag gtcgtggagg acaaggtgcg ctccaacctg cagtctgtca
    35281 ttggcagcgg gacaaagtat gactccagaa gcaccgggga gcttttacgc tcccttggca
    35341 cttctcccgc actgcaggac aggaacgggg attttgactt aaaggtgggc ttttccgagc
    35401 ctcgttcgga cggtagcagc aacgccaaga tcgccaccat cctggaatac ggcaaaagcg
    35461 gccagcctgc taagccgttt ctgaagcccg cccgatcggc atccaaaaac gcctgtatca
    35521 gcgccatgaa ggcaaagctg gacgaggagg tggagaacat ttgagtttac tttcggaact
    35581 gaaaactgtg atagaggcgg tcggtctgcc tgtggagaca ggcgtgttct ccgatgaacc
    35641 gccggaggaa tatgtggtgg tcacgccgct tgcggatacc tacgagcttc atgccgataa
    35701 cctaccggaa tatgagaccc aggaggcccg cctctccctg ttctccaggg gaaattatct
    35761 gaagcggaaa aagcagcttt ccaaagccct gcttgccgct gattttacca ttacggacag
    35821 acggtatatc gggcatgagg acgataccgg ctaccaccac tacgccattg acgtggcgaa
    35881 aacctatgaa acggaggaat gagatatggc taccattggc cttgataaac ttttctattc
    35941 aaaaatcacg gaagatgagg atggcaacga gacctacgcc actccggcat cccttgcgaa
    36001 agccatgacc gcagagcttt ccgtggagct tgctgaggca acgctgtatg cggacgatgg
    36061 cgcggcggag gtcgtgaagg agttccagag cggcaccctc acccttggcg tggacgatat
    36121 cggcgcagcc gctgcgtctg acctgactgg ggcggtgatc gaccagaacg gcgttatcat
    36181 ctccgccagt gaggacggcg gcgcacctgt cgccattggt tttcgtgcaa agaaagcaaa
    36241 cggcaagtac cgctatttct ggctgtacaa ggtgaagttc ggcatccctg ccaccaacct
    36301 gaccaccaag ggcgagagca tcgagttctc cacgcccacc atcgagggga cggtcatgcg
    36361 ccgcaacaag gtggacggcc agggcaagca cccctggaaa gccgaggtca ccgaggggga
    36421 cgccggcgtg tccgcatcca ctatcaccaa ctggtaccag gaagtctacg aaccgtccta
    36481 tgcggccgct gccgcggcat cgctggacgg tgaaggttaa ggaggtctga gtcatggatg
    36541 aaagaacagc gatggtcacc attggcggcg tggaatatga gatgcttctg actacacgcg
    36601 ccacaaagca gatcgccgga cgctacggcg gcttggagaa cctgggcgag aagctgatga
    36661 aagcggagaa ttttgaaatg gcgctggacg aaatcgtctg gcttattacg ctcctttgca
    36721 accagcccat cctcgtccat aacctgaagc acccggagga caaaaagccg gagctgactg
    36781 ccgatgaggt ggagcttctc acctccccga tggaactgac ggattacaag gatgccatca
    36841 tggaggcgat gtaccggggt accaagcgga acgtggaaag tgagccggag ggaaaaaaca
    36901 cggcggccgg gtaagcgatg aagaattgtt tacccggctt ttgtattacg gcatggccca
    36961 tctgaatctg tcacgggatg aggtgtggct catgccgttt ggcttgctta tggacctctg
    37021 ggaatgccat aagcagttta tggggatcgc aaagccgaag caggtgctga ccattgacga
    37081 tgtgattccc tacggaatct aacggggtcc ccggaaagcc atatggcttt ttggggagag
    37141 gacgaatgac ggaatgagcg agttttcgtg tttacacgga aacgagggat atgaagtcca
    37201 tgaggacgta ggaaggaggt gcagcccgtg acggataatc tcggtctgaa gatcggcatt
    37261 gagggcgaga aggaatttaa aaaggccttg tccgagatca accagtcctt caaggtgctt
    37321 ggctctgaaa tgaagctggt gtcctcgcag tttgacaaaa acgataagtc cgttcaggca
    37381 ctttccgcaa ggaacactgt gctaaataag gaaatcgaag cacagaaaaa caaggtggaa
    37441 accctgcggg ccgccctccg gaacgccgca gattcctttg gggagaacga ccgccgcacg
    37501 cagaactggc agatccagct caataacgca gaagccgcct taaacagcat ggagcgggag
    37561 ctttccgata atgagcgcgc cattgaagcc ctttcccggg aggaaacaga cgcagcggac
    37621 gctacggaac gtctctccca ggagatcgcc cggcaggagg atgcgcttgc agggatgaag
    37681 cgcgcctatt ccaatgccgt gctgcagtac ggtaaaggct ccagtgaggc aaaagaactg
    37741 gaggggcgca tctcccagct ttccggggaa ctgcgggaaa accgggagcg gatgaaggac
    37801 gccggggatg tggcggagga tttcggcgat tctctggagg acgcctccag cggggcggat
    37861 aaattaggct ccggcctttc ggtcgccacg gtggcgatgg gaaacctcat ttcttccggc
    37921 atccaggcgg cattgagcgg catccaggag cttggcagcg ccatctggaa cctagacgaa
    37981 gccaccgagg aataccgggt ggcccagggc aagctgacca ctgcctttga ggcggcggga
    38041 tacagcgggg acacggcaca gaaatcctac acggagttct ataaaatcct gggcgatacg
    38101 gacacggcca cggaagcgtc ccagcttctg gcgcagcttg cggaaaatga gcaggacatt
    38161 accaagtgga cgaatatcgc ggctggcgtc tacggtacct ttggcgactc cctccccatc
    38221 gagggcatga tcgagtccgc caatgagacc gcaaaggtcg ggcaggttac cggctccctg
    38281 gcggacgcct taaactgggt gggcatcagc gaggacgctt tcaatgaaaa actggctgcc
    38341 tgttccagtg aaagtgagcg caatcgcctc atcatggaaa ccctctccgg ggcctatgac
    38401 gaggcaagcg gcgcgttcta ccgcaacaac gaggcgctgg tggcatccag ggaaggccag
    38461 gcccagatgg acgagaccct ggcggggctt ggggagacca tctcccatgt gaagaacagc
    38521 ctccgggcgg agttcctccc ggcaatttcg gaagtcatct ccgcctttac cgacatggtc
    38581 aacggcgtgg acggggcaga cgaagccttt gccggggcca ttacggggct ggtgaacaca
    38641 gcggtttcca tgctgccgca gtttgtaaac accgggatgc agatgctgac ttctctgctt
    38701 tccggcatta tccagagcct tcccgctgtg atggagggcg cggcgcagat catcgtcacg
    38761 ctggcccagg gcatcgcggc ggcggtcccc accctgattc cccagattgt cctggtggtt
    38821 acccagattg tacagacctt gatcgaaaat ctcccgatga tcctggacgc ggcgctgcag
    38881 ctgatcatgg ggctggccca ggggctgctg gatgccatcc cggtcctgat cgcggccctg
    38941 cccgccatca ttacggcgat cgtggagttt atcgtggggg cgattcccca gatcatcaac
    39001 gccgggatac agctgttgac ctcgctggtt tccgcgctgc cggaaatcat cacggccatt
    39061 gtggcggcga tcccccagat cattgatggg ctggtaacgg cgatccttgg cagcatccct
    39121 cagatcatcg acgccggcgt gaatctgctg atttccctga tccaaaacct gccgaccatc
    39181 attaccacta ttgtaggggc aatcccgcag attatttcct cgctggtgaa cgccatctta
    39241 aacagcatcc cgcagatcat ccaggcgggc gtgcagctgt tcgtgtcgct gatccagaac
    39301 ctgcccgcca tcatcgtgga gattgtaaag gcggtgccgc agatcatcgc gggtattgtg
    39361 aacgccttta cttcctcaat ggggcagatc gtcaacatcg gcaagaacat cgtgcagggc
    39421 ctgtggcagg gcatccagag ccttgccgga tggatctggg acaaggtttc cggctggatt
    39481 tccggcatct gggacgggat ctgcagcttc tttggcatca actcgccctc gaaggaaatg
    39541 gcctgggtgg gcgagatgct gggcaggggc cttgccggcg gcatcgagga cagcgccggc
    39601 gaagcggtca gcgccgcaga ggacttaaac aacggcatcc tgggcgtgat gaacgggctg
    39661 gcggcggata tgcagtccgc agttccctct aactttgtct ttgacgcggg cgggacgctc
    39721 cgctccgcag ccggcggcat gggcagcggg ggttcttcct tcggggccct catcaccatc
    39781 cagcagatga tcgtccgcag cgaggatgac atccgcagga tttcccagga actttacaac
    39841 ctgatccaga ccggctcccg cgcccaggga agatttagta cagcttaaag ggggtgcagt
    39901 ctatgggctt ttcatacaat gacattactt caaagagcat ggggctgaaa gcaaggctga
    39961 cctcctggca ggtcagcgga cggctccgca actttaccac gaccgttcct ggaaagtatg
    40021 gcgttaccga cttcggagcg gactttgact accgggagat cgtggtgtcc tgcagcatct
    40081 ttccaaggca cagctttccc gccctggttt ccacgctgga tgacattgcg gcgtggcttg
    40141 acccggtggg cggcttaaag cagctgattc tggatgatgt gccggaccgg tatttcatgg
    40201 caagactgaa cgctgcggcg gagtgcgaac ggctcctgcg ctcctccggc agctttgagc
    40261 tgaccttttt ctgccctgac ccctttggct acgccatcga ggatgaaacc ttctctatca
    40321 cggcggaggg cagtcatacc ataacccggc ataccgggaa catggagtcg aatcccatct
    40381 accgcattga gggagaaatt acctccgggg tgggaaacca tatcagtatt acgaccaacg
    40441 ggcaggaatt aaagatcgtt aacgccaccc ttgcggcggg cgaaacgctg gtggtggata
    40501 ccgaccgcat gaccgcctat gtagaggatg aaaacggcgt cacggtgcga aacgggctgc
    40561 cgtatctgga agaactgaat tttcccacgc tttctgtggg cgacaatact gtctccgtgg
    40621 cagtgtcaaa cgctgtgttt acggggcttg aaatacaggc aaggagcaga tggaggtgag
    40681 cggcgatggc gctaaagaca atactgaatc aacagacaga cttcacaggc gagttcccgg
    40741 tcgagtgggc aaaggacggc ctgtggcgga tgaatgaatc cgctcccgat gaaaacaaca
    40801 atcttctgga ttcctccggt gcgggaagac ctgcttttat taacaactgg agcggcacca
    40861 ccgcatctat gcggagtggg cagaaaggca attactttcg ttttaacatc aataatcctg
    40921 ccacagagca gacctatctg aaggtcgcca atgacggcag cattttcgca gagcttggag
    40981 ggcgtatcct ctgcggcggc tggatgaacc ccaccaccta ttctgtggga aacacctact
    41041 gtccgatctt caacacaaga tacggtcccg gccagccaat cttctacctc tcccttttcc
    41101 gaggcaggcc gagggtcatg ctctatgaca ataccggctc cctgattctg gatgaaacgg
    41161 tcacgccgcc cttctccctg gtaaacggcg gctggtactt tatcgcctgc ctgatcgagc
    41221 cggataacaa gacggcacag tatgtggtgg gcgaccgtga aagcggcact gtgtgggcat
    41281 cggaggtttt cacctttacc ggggaactga accgctcctg cacagcggat ctgatccttg
    41341 gaatgcacgc cgattcctat tggtacgcag gtggattgga cgactggttt ttagactgcg
    41401 atacccagct taccgcagag gatttgatgg attattttcg ctcctccctg atgacaaatg
    41461 ccggggatac ctccgggagc gtagacggta tcactgagcc gggagcagtc acgctccggg
    41521 catcaagtgg tgtgtacccg tctgagggcg tgctgaccac agcggcggct gagtgcaacc
    41581 tctccggcac gggacgtgtg tctgtgacca gcgagtatat ttccggcaca acatcggttt
    41641 cccttgtgga gacatcaacc agtgacgacc ttaccgattg gagcgattgg gcggcagtcc
    41701 ccgcagacgg gcggctgact tctcccaacc gggcatacat ccgtttccgg gtaacgctca
    41761 cgacttccga tacttcccgg acgccaaagc tcatcgatat ccggctctac gacattccaa
    41821 aagcccccta tgagaagatc ggctacgcaa ggccggtagt gctggacggg aacggcgcat
    41881 gggaggcggt actggagaat gcctacgaca tcattgtcac aggcgagatc aacggcgagg
    41941 acaccctctc ctttaagatt ccttaccggg atggcaagcg tgggtatatc gacagcgaga
    42001 agaagatcca gatcgtggac gatgtgtaca aggtcaggac ggtcactgat accagggatg
    42061 cggatggcag cgccgtgacc gaggtgtatg cggaggcgga gttctatgac ctgacattct
    42121 ctgtccgcaa ggaagagcgc acctttgaag cggaataccc ggaaacggcg atggcctacg
    42181 ccttagaggg aaccgagtgg agcgttggga cggtgacggt acggacaaag cgcacctgga
    42241 ccagtacgga aaagaatgcc ctgtccatcc tcagaaatac agcagacctc cacggcggcg
    42301 atctggtttt tgactgcccc aaccgtctgg tgcatctgct gacggtaaac ggcagggaca
    42361 gcggcgcgct gtttgcctac aggaaaaaca tgaaatccat ccagcgtgtg gtggatacca
    42421 gggagcttgt gacaaggctc tacgctgtgg gtgcggaagg gatgaccttt gccgacatca
    42481 acggcggaaa agcctatgtg gaggacttca cctacacaaa tgaggtgcgc atctccaccc
    42541 tggactgctc ctccttcaca aacccctatc agatgaagga atacgccgaa atgcggctgg
    42601 cggattacgc aaagcctacc atctcctacg tgctgaatgc gatggactta tccgtgctga
    42661 caggctacga gcatgaagcc tgggagcttg gggattatgt ccgtgtggag gataaggaac
    42721 tggggctttc ggtgaccacc cgcatcgtcc gccgggagta caatctgcag gagccgtgga
    42781 acacggtgct ggagctttcc accacgctga agaacctggg cagttccgcc agcgagtggg
    42841 acaacgcggc tgactcgctg gaaggcacca gcatggtatc caacaatgat atccgggaaa
    42901 tggtcccctt caacctgctg cggaactccc gcgctgatga cgggctggcc tattgggtca
    42961 gctccggctt tgaggcagac agcgaaaacg gcgcatccgg cacggcttcc tttaaagcgg
    43021 agggcgtggc gggcatgacc aaaagcctct cccagaccgt ttatcccgcc aaccgctcca
    43081 gttacaccct gtcggcgcag atcggctcgg aaaatctgga gaagctgagc gatgacgccc
    43141 aggtgggcat tgaagtggtc attgagtatg aggacggcag tacggaatca agattcattg
    43201 acctgtactg atggaaggag gacgaaatgg catacttatc atccacctcc gccaaaatca
    43261 cgccggagaa ctactccgcc agggtcaagt ccatcacggt gcgggtgtgc gtcaccaact
    43321 gcgccgggat actttatatc acggacatcc tcctgcaggc aggggcggtg gccacgggat
    43381 gggtaggcca tccctgtgag atgaagtgga cgctggatgg gtaaggtcgc ttttatccgg
    43441 ctggcggagg tcattaaccg gaaacaggat atgcgtgtcg tgagcgttac cgtgaagcct
    43501 accatcacgg actgctccgg cacgatttat tttaccgacc tgatgctcca agaaggaccg
    43561 gcgctgacgg gatacacgcc ccataccgag gtgttcctcc aaaagttccg ggagaacggc
    43621 gaggtcaagg cacccgtctg gttcaacggc gtggtgcggg gcgaggaaac ggtcatcctc
    43681 tttaaccttg gggagacctc cgctgggctg gatgtgcata tctatccgaa gtccgacttg
    43741 gaagctgggg cggtttccct ctgccagggc gtgggcggcc agaaggtatc cttccctaac
    43801 gccgtcccag ccgaagcgga tctcgccctg ctggcttcta ctcggcagtg tacgaagaac
    43861 ggcagcccgg agaaaaaaga gggcttttac cagtacagcg ccgcctggga ttccaagcat
    43921 aaggtgacgc tcccggaagg gaaaacggcg cgggtgctgt ttgagatgca ggaaatgcag
    43981 gatggaggtg agccgatctg atggacacac tgaaaggcaa acagatcatg gtatggacgt
    44041 tcatgggcaa tgcccggatg tacgaagccc tccgggacta cggcgaccgc atcagccaga
    44101 tcgggctgtt ctcctttaag gtaagagcga ccggagagat ctatgaaagc ggcgtggcga
    44161 tctcggatat gctcacctac atcaataaat ggccccatat caagtggctt ttgaccgtgg
    44221 caaacgatgg cgcaaacagc atcttccgcg ccctgcggga taacacggac ggggcgcagg
    44281 acaggtttct ctcggagatc gtccgcatca tggagaaata tccctggtgt gacggcattg
    44341 acatcgacct ggaacgtggg gacggctatt ccacccatac ggagtccacg gcgatgttcc
    44401 gcaacatcta caatacggtg aaagactatg accctgcaaa gcacacgaac atctgcctgc
    44461 cggggatgac ctcggtaaac ggctcggtgg gcggcgagaa ctggtgtgtt tacggcgact
    44521 taaacgctta ctgcgatacc gcatccatca tgtcctacgg catggcatgg gcaggctccg
    44581 caccgggacc ggtttcccca agaagctggc tggagggcat ctacgactac gccacgaagg
    44641 tcatggaccc ggataagatc ttcctcggta tgcccgccta cgggtggaac tggcagattt
    44701 acgatacgcc ggagaacctg ggcgagacct accggggcgt ttccaacacc tactacgccg
    44761 caaggtactg gatgacggga gcgtacaact tcacaggtga cgcgccgccc cagccctttc
    44821 tccctatcgt ggcctattgg gatgattatg acaaggtgcc ttacgccttt ccccatgtct
    44881 acgattacat ggaaggagcg gacgccgttt cccgcgagta ccctcagctt gcggacacat
    44941 acaaccgcag gcgctaccta actgcctacg gcaaggagca gaaaaccgag ttcggaacta
    45001 ttttcatcga ccgggacgct gacggctact ccagcgcgtc cggcattgtc tccattgaaa
    45061 acggtatcgc aaccctgggc gataacggct cggtgaccta cagcttttcc gtgaacacgg
    45121 cgggaactta cgatgtggcg gtgcggctct gctacccctt ctgggacaag aacggcatct
    45181 atgcggcgct ggacggtagc acaaagcact tcacggaaag ccgcctgtgg tggccgcact
    45241 ggcggagtac cttctgggcg tccctcgcaa gcggcgtaac gctttcggcc gggacacaca
    45301 ccattaccat ttcagtggat gccaaaggcg tccagtttta cggcttccgg gtctgctcgg
    45361 ctttttccga ggaacctacc gccggggagg ccactttcgc ccttgcgccc aggagcttta
    45421 aggatgtgga cggcaatatg gctgtccctg acaaaggctt caagctgacg ctggagatgc
    45481 tccgcaggaa gccggactcg gcgcttgtat ggtatgagga cttccaggat tacggcgtgc
    45541 tggagacgga ttactggacg gtgcgctccg gctccttcga ggtgtggcgg tcggatgaat
    45601 attcgatgga gcgtgtctac tcccagctgg aagggcatgg ggagcttgcg tggcagtatg
    45661 acggcttttc ggaactgcat ctgcgggcca ggctggcttt cccggcaaat gggagcggac
    45721 gggccggtgt gttctgcggc agcctgttct gctgcttaaa ctacgacacc caggcggtag
    45781 aactgtacaa cggttccacg ctccttagca gctacagcca ggagatcgca aggacttcat
    45841 cggcggattt gcgggtcaat ccctccatgt acacggtgga gatgcgtgtc cgtggaaacc
    45901 gggtgcgggt gtattccggt tcttcctaca ccctgcgctt tacggcgaca gccagcggtt
    45961 tttccggggg ctatgccggg taccgctcgg acaacaccac ggtctgtgaa ctgctgcggc
    46021 tgggggatgc ctggacctac gagccgtatg agcggtttga tgtggagatg ccggacggca
    46081 gcttcaaaag ctacggcagg atcagccgtt tcaactgtac ctgggatgag gagttccagg
    46141 tgttcacgct gacctccgat gtggaggaaa cctccacccg gagcgaagac atttccctgg
    46201 attatgactt cttccattcc gaccttttag agatttcctg cggcgggaac tacacggcaa
    46261 ggatcatacc aaaggacatc aacatctgga tctcccggct gtttcttgga gatgcggacg
    46321 gcttttctat cctctactac caggacgtgg attccctggt ctattgggcg aaccaggcgg
    46381 cgtaccgctg gaagctccgg gggatgtgta tgtggtcttt gggtcaggag gatatgcggc
    46441 tgtgggaatg gctgccgaaa cagatttgac aatttaatct tttgggaatc agcgactact
    46501 tttcggagcg gccgcttttt tcatacacaa aaccaagaga aacggaggtt accaacatga
    46561 aggaattttg gaatgtgatt cagatggtgt tcaccgctgt gggagggtgg cttggctact
    46621 tccttggcgg caacgatggg ctgctcatcg ccctggtggt gtttgcggtg gcggactaca
    46681 tcacgggcgt aatgtgtgcc gtatctgaca agaagctcaa cagccaggtg ggattcaagg
    46741 gtatctgccg caaggtgctg atcttcctgc tggtggggat cgcccacatc ctggacgtgc
    46801 aggtcattgg aacaggctct gtactgcgca cggcagtgat cttcttttat ctgtccaacg
    46861 agggcgtgag cattattgag aacgccgcac accttggact gcccatcccg gaaaagctga
    46921 aagcagtatt ggagcagctc catgacagag cggaaaacga aaaggaggac gaatgattat
    46981 ggcttacaca aacagttcac tggtatctta cacaaaactc agccccaacc actccgggca
    47041 gaggacgcac tccatcgacc gtatcacgcc ccactgtgtg gtggggcaat gctcggtgga
    47101 aacactcggc agtatctttc tgccgacttc cagacaggca agctgcaact acggcatcgg
    47161 cgttgatggc cgtgtaggta tgtatgtgga ggagaaaaac cgctcctggt gttcctcttc
    47221 cagcgccaac gatcagcggg ccatcaccat cgaatgcgca tccgacacca cagagccgta
    47281 tgcctttaag gatgtggtct accagaagct gatcaccctt tgcgtagaca tctgcaagag
    47341 aaacggcaag aaaaagctcc tgtggcttgg cgataaggac aagacgctct cttatgagtc
    47401 gaagtccgat gagatggtgc tgacggtgca tcgctggttt gccaacaagt cctgccccgg
    47461 aaactggatg tatgccagga tgggcgatct ggcggaaaag gtcacggcac agcttggcgg
    47521 ttcagctgat actcctactg taaccacgca gctttaccgt gtccgcaaga cctggtctga
    47581 cagcaagtcg cagaaaggcg catacaagat cctctccaac gccaagaagt gcgctgatgc
    47641 caatccggga tatagtgtgt ttgatgtaaa cggtgtaaac atctatacat cgaaaacaat
    47701 agccgcagag gttccgttcc tcgtaaaggt cagcatctcc gacctcaata tccgaaaagg
    47761 accgggaaca gactacgcca agaccggtaa gtttaccgga aaaggggtat tcaccatcgt
    47821 tgaggtgaag tccggcaagg gctccacagc gggctgggga cgcttgaaga gcggcgcagg
    47881 ttggatctcg cttgactacg cagccaaaat taaataactt aacctgctga aagcccaccc
    47941 taccataacg gttcggtggg cgtatttttt tcggccaaaa cgggctacct tgtccagatg
    48001 ggtcattgag ggaaaccctc ggaacggagg agccacaatg acaaatcagc aaaaagaaca
    48061 gattaccgcc ctacgctcac agggatatgg atacgccacc atcgccaagg cggtgggact
    48121 gaagaaagat accgttgtcg cattctgccg gaagatgggg ctgaccggca caaaggctgc
    48181 ggataacagc cgcattgaac tggacgccgg attctgcctg cagtgcggcg ctttgcttac
    48241 gcagactccc ggaagaaagc gagtcaagtt ctgctcggat aactgccgta ctgcctggtg
    48301 gaatgcacac ccggaaaagg tcaaccgcag agccgtatac cacttcacct gcgctcactg
    48361 cggaaagccc ttcactgcct atggcaatgc aaaaagaaaa tactgctctc acgcctgcta
    48421 tatcgcagac cgctacaaag gcggtgacgg gcatgagtga ggacaaattc cgttctgaaa
    48481 tgagctacct tgccgccctc tccatcgcaa agaatctccg agaaaagggg cttctgagcg
    48541 aggaggaata tgccgtaatt gatacaaatc tgagggctga gttttcgcca tctttgggta
    48601 cattattatc ggaaaatgac ttgatataat cggctttcag agtgatatat agtgtcggaa
    48661 aggagtgatt tcatgcggat tgtaaataaa atcgaagcga aaacaccgca gataccgcac
    48721 cgcaaaaggg tcgctgccta cgcaagagtc tcaatggagt ccgagcggct gcagcactcc
    48781 ctttcggcac aggtcagctt ttacagcagt ttgattcaga gcaatcctgc ctgggagtat
    48841 gtgggcgtat atgccgacaa cgggataacc ggcaccaaag ccgaagcccg tgaagagttc
    48901 aatcggatga ttgccgactg cgaagccgga aaaatcgaca ttgttctgac aaagagcatt
    48961 tcccgtttcg cgcgcaatac cgttgacctg ttgaatacgg tgcgcaggct caaggagctg
    49021 ggcgtttccg tccagtttga aaaggagcgc atcgactccc tcaccgagga cggcgagttg
    49081 atgctgaccc tcttagcatc ctttgctcag gaagaaatac gcagcctgtc ggacaacgtc
    49141 aaatggggta cccggaaacg atttgaaaaa ggtatcccca acggccgctt tcaaatttat
    49201 gggtaccgct gggagggcga tcatctggtc atccatgagg aggaggcaaa aatcgttcgg
    49261 ctcatctacg acaattacat gaacggtttg tcggcggaga ccacagaaaa gcagcttgcc
    49321 gagatgggtg taaaatccta taagggacag catttcggta acacttccat acggcagatc
    49381 ctcggaaata tcacttatac gggcaacctt ctgttccaga aggaatatgt ggcagacccc
    49441 atcagcaaga aaagcaggat caaccgcggg gagctgccgc agtatttcgt ggagaacacc
    49501 cacgaagcca tcattccaat ggaggtctac caggcggtgc aggccgagaa agcgcgccgt
    49561 cgtgagcttg gcgccttggc aaactggagc attaacacct cctgctttac cagcaaaatc
    49621 aagtgcggcc ggtgcggaaa gagctatcag cggtctaacc gcaaggggag aaaagaccct
    49681 aatgccaact acaccatctg ggtctgcggt acccgaagaa agaccgggaa tgcgtattgt
    49741 caaaacaagg acatcccgga gcagatgctc aaagatgcct gcgctgaagt catgggactg
    49801 gatacgtttg atgaaatcat cttttcagag cagatcgacc acattgagat tcctgctccg
    49861 aatgagatga ttttctattt taaggatggc cgcatcgttc cgcaccactg ggaatccacc
    49921 atgcggaagg actgctggac ggatgagcgc agagccgcca agggacggta tgtgcaggag
    49981 catcagctcg gccccaacac ttcctgcttt accagccgca ttcgctgcga cagctgcggc
    50041 gagaactacc gcaggcagcg ttcacggcac aaagacggca gctttgattc cgtatggcga
    50101 tgtgcgtcag gcggaaaatg ccaaagcccc agcatcaagg aagatgccct caaaaacctc
    50161 tgtgcggacg ctatgggtct ggaggaattt agtgagacgg ttttccgtga gcagattgtc
    50221 tgcattcaca tcacggctcc atatcagctt tccatccgct tctttgacgg gcataccttc
    50281 gagacggcat gggaaaacaa gcggaagatg ccccggcata cagaggagcg aaaacagcat
    50341 atgcgagaag taatgataca gagatggagg gaaaaacgtg gcgaaagtaa cgacaatacc
    50401 tgcgacgata agccgattca cggcaacgcc gatcaatgaa aagaaaaagc gccgcaccgc
    50461 cgcctacgcc cgtgtttcca cggacagcga ggagcagctc accagctaca gcgcccaggt
    50521 ggactattac accaactata tcaaaagccg ggacgattgg gagtttgttt ccgtgtatac
    50581 ggacgagggc ataacgggta cgaataccaa gcaccgcgag ggattcaaac gcatggtggc
    50641 ggatgcactg gctggcaaga tagacctcat cgtcaccaaa tcggtcagcc gattcgcccg
    50701 caatacggta gatagtctga ccacggtgcg ccagctcaag gaaaaaggcg tggagatcta
    50761 ttttgaaaaa gaaaacatct ggaccctgga cagcaaggga gaactactta ttaccatcat
    50821 gtcctcactg gcgcaggagg aaagccgcag catttcagag aactgtacct ggggacagag
    50881 aaaacggttt gctgacggga aggtcaccgt gccattcaaa cggtttctcg gctatgatcg
    50941 gggacccgat ggcaatctgg ttttgaataa ggacgaagcg gttatcatcc gccgcattta
    51001 cagtatgttc ctgcaaggca tgacgccgca cggcattgcc gccaggctaa ccgctgacgg
    51061 cattaaatcg ccgggcggca aggataaatg gaacgcagga gctgttcgca gcatcctcac
    51121 caatgagaaa tacaaaggcg atgcgcttct tcaaaagagc tacacggtgg atttcctcac
    51181 caaaaagaaa aaggtcaacg agggagagat tccgcagtac tatgtggagg gcaaccacga
    51241 agccatcatc cagccggagg tgtttgagct ggttcagcag gaactggagc gtagaaaaag
    51301 cagccgtggg cgacacagcg gcgtccatct tttctccggt aagatacgct gcggacaatg
    51361 cggcgagtgg tacggctcga aggtctggca ttccaacagc aaataccgcc gggttatctg
    51421 gcagtgcaat cataagtatg acggcgagga aaaatgctcc acgccgcact tgacggagga
    51481 tgaaattaaa gcgatgtttg tctcagcggc aaacaagctg attggcaaaa aagcggcgat
    51541 tatctcgccg ctccgaaatt cgctggacgt tgctttcgac acatctgcac tggaaaccga
    51601 agtcgcagaa ctgcaggatg agatcatggt cgtctcagac cttatcgaaa aatgcatcta
    51661 cgaaaacgct catgtggcgc tcgaccaaac ggaatatcag aaacgctatg acggtctcac
    51721 cacccgtttc gataccgcga aggctcggct ggaggaaatt gaagccgcgc ttgccgacaa
    51781 gaaatccaga cgggcggcaa tcgacgcctt tctggacacg ctggcgcagg ctgacccgat
    51841 ggagaaattt gaccctgccc tctggtgcgg gctgattgat tatgtaacag tctatgccag
    51901 ggacgatgtg cggtttgctt ttaaggacgg gcaggaaatt aaagcataag gataaaaaag
    51961 atactcctca ctaccattac ggcggtgagg agcttttctg tgcatataaa aacagccgtt
    52021 tcggatttta caccgcaacg actgatttta caccgcaaag gcacactttt taattattcc
    52081 acggcattgt attaaactca gtaagattat ttcaaaaaca ctaaagaatc gtcgttcttt
    52141 ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg tgtaaaattc
    52201 tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat aagcatcccc
    52261 gatcacgtcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttaa tgacttcggt
    52321 aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga gatccacatc
    52381 atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg ctttgtttag
    52441 atcattcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga aaagatactc
    52501 tggcgaaatc gcatacccca aaatatcaac gattgttgca atcagatctt cttttgattc
    52561 ttcatctgaa agtaattcgc gatacaacat cgtttgtttg cttaccgtat catattcctc
    52621 taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata aatatttata
    52681 gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttgc tacggagatt
    52741 atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac actcctatct
    52801 cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag aaaaagcaaa
    52861 aaaaaaaaga aagaaactta ttcttgctaa taaactacgc gaaaggagtc taacgttgtt
    52921 tgatataact tagactgatc gttaacttat tttttatatt tctacatagt tatctttaga
    52981 accatttcag atacatatga aacaattaca tatgattttt tcataaaaaa acagtactgt
    53041 ctagtagtat aaaatataca aatttcttaa aaattttttc actaaaatca ctacctgaaa
    53101 cagtctaaaa cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac
    53161 aaataaatat agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc
    53221 tactggagat gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga
    53281 tggaagaatc attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg
    53341 ttcttaaata ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatatct
    53401 ttaccaactt tctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat
    53461 aaagattgag catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca
    53521 tttacaagtt tccaagtagg tggtatagct gtgtatgttt ctacaaatga attaagtttt
    53581 tcattaagtg acttctcttt tatatttcca tctaaaaact ctgtatatat ggcttgagct
    53641 atactaaaac aaagctggcc ttgttctaat aattttacat cattagttag taaagctgtg
    53701 ttcacgttat caatcgcttc aagaagagta tctttggtgt ccctaacagc tttaggtgtg
    53761 ccttctggaa tttccctact tgatacctcg tccattttat cttccataaa ccccttaaaa
    53821 tcatatcttt ctcccaaatc catagaagtt tttgagaaag ctttcatttc tgagtctatt
    53881 tcttcaataa tatttaacat gtctgataca tattgtttag gctgattcgt ttttaatcta
    53941 tatgtggtaa atatctcaat tcctccaatt atcaataaaa ttgccaaaca aatccataat
    54001 aattttttct ttttcatttt tcctcctata cactgctgtt ataacagtga ctaatgtaaa
    54061 gattttccca gcaatcatca gactattatt tacagggaat caaaaaaact ccttagtaag
    54121 cttacacctt taggagttcc tgtttgaacg gtatgcataa tacatttaaa tcaatttaac
    54181 attaacacct cagtatatgg tatctctaca tatacacggt ctttttttgg atagtactcg
    54241 acatttatat tgatagcact tccattgtct aacgtagctt tagcccggta cgtgttatca
    54301 ccattatcat gagtgatcgt cacatcatca catgtccgat ttaagttata ctgttcttgt
    54361 aaaatctgag ttaccaatgg tacagatgac ttctctagtc cagatgaacc cgtcattctt
    54421 gaaaaaatta tacctataat agccaataca acgataccta taatgacaaa cttcttattc
    54481 atatttactt tattcaactt tttatctcct atctccaaaa tgattttctc tcatcagtaa
    54541 ctcattgaca tccccaatag tacaccaaag atggctccag ctacccctgc tatcatcaaa
    54601 atcgtaccca tttgttcatc tttctctcga tataagtaac ccataataac agcaacagca
    54661 ccaaatacca caggaataaa tgctaatgat actaccatag aaatccaacc aataatcaaa
    54721 tacagcttcc ctgtctcttg tccatgtacg atttgttcct tcatttcatt aattgtctgc
    54781 tcagtacttt tctttactgt gtctgttccg gttgtctcat ctgtttcttg taatgctccg
    54841 caaaactggc aatacttact ttcatttgtt atttctttac cacattttct acaatacatg
    54901 tcttctcctc ctaaaatcat tcaattgctt attagctaac tgtttacttt attaaataca
    54961 tcagaaaaca gctcatttat tggtacactt aatatctcag aaagcatttt catttgatag
    55021 ggtttaggaa tcgttctccc tgtttgccac gaacttacac attgctgtgt aacattcatt
    55081 tttttggcta actcagtttg ggaaagtcct ttttcttttc tgtatcggct gagattactt
    55141 acttttttca tgattctccc ctcctgtttt attatataca acaatatgat gtatttcaac
    55201 tcataaaata tataaatact tcaaatgtca ttacaaatac aacaacttgt tgtactctac
    55261 tgagaagggg ggtgtattaa tgaaacagaa gcaatatgca aaggtcatta gagaaaagcg
    55321 aaaagcactc gggtggacac aaaaagaatt agctaaaaaa atcttctcta cacaacaggc
    55381 agtagcaaga tgggaaaatt ctgtaacaga acctaacctc gatagcttaa cagctctttc
    55441 tagagcccta ggaacccctg taagccattt tctagacaat gttgacatag attatgagga
    55501 agagtttttg gctctctatc gctctcttag tactgaagat gcaattcgaa caattgacta
    55561 tatgaagcta ttaaaaaggc aagaaaatga acgcaatcag ctattgaaag attgaaaaat
    55621 aaataaccca ccgaacttta acggtgggtg tattgagatg aaataagatt aagagctatg
    55681 gataacctgt ctagtttaat ataattgggt ctcttctatt ccttactctc cgtccatcat
    55741 ctcttgttac acttgtaaat tgataaatag gaactaattc aactcctgat aatatcaatt
    55801 cttttattac ttcatctatc cctgcccctt tcgctagata tactaaaaaa ctactgcttg
    55861 ctttcacatt gtatagtttc ttaatatcct tagaagctcg ttcaagagtg aatccttgtt
    55921 taacaaagta gcctctgttt tctattaaag catttttcac gctgttatcc ctgacatttc
    55981 gtaggcgttc acaaacagtg ttaataagtt ctttttctgt cattagatat gtcccttgcc
    56041 acgataagcc ctgtaaaact tcatgtgctt catctaaaat caattgtttt tccttgctaa
    56101 gttctgatcg ggaattttga ggaaatactc ttttagcctc tttttcgtta aaacatatct
    56161 taacccattc ataagaaaaa tattgatatg taaaatgaaa tttttccaga agtggaagga
    56221 tatcttcatt caatttttga atcacagaag ttgcattaaa aacgtctaat cgatagacat
    56281 tgcttctctt atgtttaaag tttcctaacg ctttctcttc ctttaaccta tttaggtatt
    56341 cgagcatctt caaaggaaca tcttcatcat ctaatttttt tatcaatcct ataaaagtca
    56401 taagtgatag taacttatta catttatcta tgtcataagt tttaccatag taagaactca
    56461 tttttttagc taattgttcc cccgacaaaa tacaagtcac actttcctta ctatcatcaa
    56521 aaaaattaac atcagaaagc aagacatcat agacacgtcg catttcctct agataattcc
    56581 ttattgtcct gaaaagtttg tctttcattg attcaagagt gtcaaaaagt acttttgatt
    56641 cctctttcaa ttttcgaaaa tgtgtaggat acaagtctag gttctctaat agccaattaa
    56701 atgtttctac ccgactgtct ttctttaaaa ttagcatcac aactccaata aaattttcac
    56761 tgatatcaca attactggaa tgacaataaa aaatggaatg cccagtttta ctggtaaaaa
    56821 tactagcaga gggattatga tcgttgtgaa acagacatct taagttttta cttgacttaa
    56881 gtaatttatt catatcaatg gttaacaatc tttcataaat ctcattaaaa gacatgtcgg
    56941 aggaatcaaa caggcagtct ttaaaccact gtttcatttc ttctttgtcg tcatttttta
    57001 tcaacttaac aaaatcggtt tcattgacta gttcatactc tctccctgaa tcactttgac
    57061 aatttttaga acatgtcatg gtaaaatcaa tcggaagctc agtaatatct aacatgttgt
    57121 cttcattaat tattatcact gcatctgact tatatcctcc aaaaaacatt ctattcgaag
    57181 catttacatt gtcatcacac ccggggatgt tctttttcaa gtattcgtaa acagcaacca
    57241 ttttaccata gtcagtaatt gcttctttta atagaaagac aatcttgtat ttttctagat
    57301 tttcagagta ccgaatactt ttttgtatcg caaacgcaga attatgaact aaattgatag
    57361 ataaaacctt ttctatgctt aagtatctac tatcatcttt actcaaatct actaccttcc
    57421 ccttctcatc tttaacagtg ttatcgacat ccagaataat tactctggtt tccacaaaac
    57481 tctccttgtc attgccttta ttctttgtct tgcttggaat aaatgattgt gatgaattta
    57541 gtatctctac aaactcatct cttgtaactt ctctatagtt atctaaacac ctatctctta
    57601 catcagtttt ttcttttttt gtaactggct tctctaataa tttttcttta tctacataaa
    57661 ttggaatatt aatcattgat gtttccattt gtgtttcctc cttataaaat tctgttaaag
    57721 actaaaacat acagataata aaaacaaact ctttattact atatgtttta gtcttatttg
    57781 tttactcaac ctttataccg ttccatgaaa ctatcaatat cagatttttt aaatctaatg
    57841 accgtatcaa tttttataac aggtaacccg tattgagaaa catattttct aatcgttcct
    57901 actgatacac ctagatattc acttgcctgc tgatagtcca tatactctag agtgactcca
    57961 gaaataatgt cttctagcat aggctttagc actgtaatta tctctttcac ttgttgttca
    58021 acttgtatag aaacaatgct atcaatgatt tgtagagaca tatttccccc tcctttcgtg
    58081 attgcttgaa caaattttct aagaattatc ttcgtatttt ttgctcaaaa taaaagtatt
    58141 ttgttattaa ccgattaatc aaacaaaaaa tgattaccag ttcatcgaca tacacataat
    58201 acctcgataa aaaaggggtg tcaatagttt tgtaaaattt atttacaaca cccctttttt
    58261 gaggtatact aattttagga ggtgtggtca atatgataaa acgtccacca ataaattatt
    58321 tagaacgaaa aaagatactc ggaacaaaaa taaaagctat aagaaagagt aaaaaattaa
    58381 cacaaccagc atttggtctt atgattaaca acggtcaatt aattgacaaa aagactatct
    58441 acgaatggga gaagggaacc tatttaccaa taccagagcg attatcccga attgctgatt
    58501 tgggaaatat gagtatagag gagttagttt gtggcaatgt agaagaatat attttgggaa
    58561 ttattcttta tagagatagc attgtactag atggtataac atttccagat aaaaacctgt
    58621 tccagcatct tcgtcaacag ttcccaccag tacactctaa tttagataca tggctggata
    58681 gatattccaa attagaacca gagatgcaag aatttattgc taacaagact tgtaataaag
    58741 ttaaaaatga aaaaattagt cttttcaata tcttaaaaat agaagaatta ttcatcaatg
    58801 ctattgtaga agaatttgac aataacattc ttttcctcac tagcagtata gaagaactac
    58861 tagaaagaat ggtagatgaa tggctaccta ttcaattgaa agacatgtct tatcccgaag
    58921 aagctgtgag agaaattact gataatataa acaagctaga acaaacgatt tcatcaattg
    58981 ggaaaaaata cactaagaaa aaaatgaagg gaggtgatac catctagtgg taagtactcc
    59041 ttgtaaacta agaggagatg atttcatggc aaaaatatca aagtacaaaa agaaaaacgg
    59101 tcaagtggcg tggatgtata aaggacacat tgccaccgat ccacgaactg gtgaaaaaat
    59161 aaatactacg agaagaggtt ttagtagcaa acaagaagct caaaaagatt atgacgagta
    59221 cagacatcaa atattatacg gtgttaaaaa gaaagcacct gatatgacat ttgaagacct
    59281 gtataatgaa tggataactc atcagcgtac cagtgttaaa gcttcaacaa tcgcaatctc
    59341 agtaagatac gcaaataatc aaatacttcc agcatttggt aagcttaaac tatcgaatat
    59401 ctctgtccca tattgtcaaa aagtggtaga tgaatggcac agtaaatatg aaagttacga
    59461 ttatatgaga aaacagactg cacaaatttt acgctacggg gttgcaatgc aatatattga
    59521 caataatcct atggaaaaaa cacttcttcc acgaaaaaaa gagtatgaga aaaatcgtaa
    59581 attttactca aaagaggagc ttaataattt actggatgct tttaaagatt ttggtaacat
    59641 gaaacaatac gccttcttca gattattggc atacacaggt atgagaaaga gtgaagtact
    59701 cgctttacag tggaaagaca tagacacgtt taataaagaa ctacatgtca ataaaactct
    59761 tgctgtagat gaatttggta aagttataat tcaatctccg aaaacaagag cttctagacg
    59821 agttatctct ttagatactg agactctttc tatcctcaac aactggaaat tacagcaaaa
    59881 agaagagtat ttaaaacttg gatacaatac tagctctaaa gagcaacatg tttttacaac
    59941 agttaaaaac actctataca ttccaaacac tgttaatgat tggttaagat atattttgaa
    60001 aaaatataac ttgcctcgta ttacacctca tgggtttaga catacacatg ctagtttatt
    60061 attagaagca ggagaatctg tcaaggtagt tcaacaacga ttagggcatg aaaatagtaa
    60121 agtgacatta gacatctatg cacatattac aaataatgca cctaagaaaa caggtcaaga
    60181 ctttgctgat atgatggctc atcagtaaaa gagctggtca aaatctggtc atttctagta
    60241 tacaggcaaa acaaaaaagc tagaaccctt atatatcaag ggttctagct cctataacag
    60301 gtttacccaa cactgccttc catctcataa ctgatcaatc gattgagttc gactgcatat
    60361 tccattggaa gttctttggt gaatggttca acaaagccca tgacgatcat ttcagttgct
    60421 tcagattcgg tcaggcctcg gctcatcaga tagtagagtt gttcttctga gatttttgag
    60481 actttggctt cgtgttctaa agagacttgg ctgttgtgga tctcattgaa tgggatcgta
    60541 tcggatttcg accattcgtc catgatgatc gtgtcacatt cgatatgaga gatcgaaccc
    60601 gcactgtttt tggcaaaggt tacttgtcct cgatagttta cttctccgcc gtcttttgaa
    60661 attgatttag agacgataga gctagatgta tttggtgcat tgtggatcat ttttgcaccc
    60721 gtatcttgga tttggtttgc tcctgcaaag gcgatggaga gcatggtgcc tcgtgcacct
    60781 tttccatcta agtaaacact tgggtatttc atcgttgttt ttgcacctag gtttccgtcg
    60841 atccattcaa cggttgcgcc ttcgtatgct tttgctcgtt tggttactaa gttgtacaca
    60901 ttgtctgacc agttttggat cgttgtgtaa cggcaatacg catcttttct tgtaaagatt
    60961 tctacgatgg cggcatgcag gctgttactt gaataagtag gtgccgtaca accttcgaca
    61021 tagtggatgc ttgctccttc gtcaacaatg atcagcgtac gttcgaattg ccccgtgttt
    61081 tccgcattga tccggaagta cgtttgtaat ggaacatcga ctttgacgcc ttttggcacg
    61141 taaataaagg tcccacctga ccatacggct gaattcagcg ctgctaattt gttgtcggtt
    61201 ggcggtacaa gttttgaaaa gtactctttg aataattcag ggtattcttt caaagcagag
    61261 tcagtgtcag taaagacgat tcctaacttt tcgaattcat ctttcatatt atggtacacg
    61321 acttctgatt catattgtgc ggaagcaccg gctagataag ctcgttcagc ttctggtata
    61381 ccaatctttt caaatgtatc tttgattttt tctggtacgt cttcccagtc acgagcgggt
    61441 ttgtcacttg gtttttggta atatttgatg gcatcgaaat cgatgtctga aaggtcgggg
    61501 ccccatgttt gcattggcat tttgttaaat gtttctagag atttcagacg gaattcaagc
    61561 atccattctg gttcgccttt gattcttgac atctcacgga tcacttcttc tgtcaagcct
    61621 tctcctgtac tgaaaaccgg ttcgacatca tcgtgaaacc cgaatttata ttcttctaat
    61681 tctggtacgc ccatgttctc actcctcttc cttgtggtaa tgatggaaag ctgcggtacc
    61741 atcgttttcc attgcttttc ctagtgcttt ccaggcaagt gtcgcacatt tgatccgagc
    61801 gggaaattta gctacaccac caagcatagc agcatcgcct aattggtcgg gatctttgac
    61861 ttcttttcct tggacaagct gtagaaagtc tacagttaaa tgttctgctt cttctattga
    61921 ttttccgatg acggcgtctg tcatcatcga ggcacttgcc gtactgatgg agcagccact
    61981 gccgtcaaag gcgatatttt tgatgattcc gtcttttaca tcgacttgca attcgatcac
    62041 atctccacat gtggggttgt tcaattcgat cttgcttgtc gagtcagtca agcttccacg
    62101 atgatgcgga tgggaagaat gatccaagat cacttgacgg tataaattgt ctaatttaga
    62161 tagtcccatg ttggaaaaac tcctttgttg ccatgattgc ttcaactaaa cggtcggcgt
    62221 cttgcgttgt gttgtagaaa taaaagctcg cgcgagcagt cgctgatact tcaaggtact
    62281 tcaatagcgg ttgcgcacaa tgatgacctg ctctgacggc taccccttcc atgtctaaag
    62341 ctgtcgcgac atcatgggga tgtaacccat ccaaattgaa tgcaacgact cctgtccgaa
    62401 aattcgggtc ttgtggacca taaacagtca atccatcaat tgcttgtaat tttggcaaca
    62461 cataagcggc tagttcttct tcatatcgat ggatattgtc cataccgagt ttttgcaaat
    62521 aatcgattgc atggcctaat gcgatagcgc cggcgatatt tggtgtccct gcttcgaatt
    62581 tccaaggtag ttctttccat gtactgtcgt ataattcgac aaaatcgatc atttcgccac
    62641 caaattcaac aggttccatt tcttctaacc attttcgttt accatataaa acaccaatac
    62701 ctgttggtcc gcacattttg tgtccgctga aagcaaagaa atcacagtct agtgcttgga
    62761 catcaatggc catgtgcggg gcagattgag caccatctac tacgatgacc ccgccgtttt
    62821 catggacaag ttcagctaat tcttgaacag gattgaagac acctaagaca ttggatacat
    62881 gagcaatgga gacgattttg gttttgtccg taatcttttt cgctgcatcc tccatatcca
    62941 ggaagccttc ttctgttagt tcgatatatt tcaagacagc acctgttcgt tttgccaact
    63001 gctgccacgg gataacatta gaatgatgtt ccatgtaaga gagcactatc tcgtctcctg
    63061 ctttgatata gcggtcgccg aaggttctgg ctacccaatt caagcttgtc gtcgtaccac
    63121 gagtaaacag cacttctgct gtttcttttg catggatgaa cgcccgtact ttttctcttg
    63181 cttgttcata atcatgggtc gcgcgttctg ctaacgtatg gacaccgcga tgaacattgg
    63241 cgttatcgtg ttcgtaatag tacgctaatg cctggagtac ttgttttggc ttttgggtcg
    63301 tcgctgcatt atccagataa actaaaggtt catcattgac gtcttgaaat aagataggaa
    63361 aatcattgcg tagcttttta gcatcgatca tccattcaac ttcccttcga ttacttcgac
    63421 taattctttc cgaacttgtt ctacagggat tgctgttaag acagaaccga ggaatcctcg
    63481 aatcactaag cgctctgctt cttctttatg caatcctcta ctcatcagat agtacatttc
    63541 ttctggatca actcgtccta cactggcagc atgtccagca gttacttcgt tttcatcgat
    63601 caaaagaata ggattcgcat caccacgtgc tttatcagaa agcatcaaga cacgactttc
    63661 ttgttgggca tctgcacctt ttgccccttt caagatgtgg ccgataccat taaaggtcaa
    63721 tgttcctttt tctcggatca ctccgtgttg caaaatatgc ccgatcgtat gaggggcttt
    63781 attggttact cgtgtatcga ttccttggat ttgtctgccc gagctgattg ccacgatttt
    63841 gacttctgcg tgtgcacctt ctcctgctaa atctgagtca aagtcagcga tcacatgacc
    63901 gtcattcatg acaccaattg cccagtcgac agaagcatct cgtaaaatgt gcccacggcg
    63961 attcatataa gaagtaatat tttctcctaa ttgatctacg gctgaatatt tgatttttga
    64021 accagcttta gcgatgactt ccacgatgat attacctgat gattttgcta cttgttcttt
    64081 ggttgtctgg aaacgttcca gataagaaaa ttcactatgt tcatctgcca cgatcaacac
    64141 atgcttgaag aagtgctcat cggatgcgcc atcttgaatg aataaggact cgagtggttc
    64201 atcgatcacg acatttttag gcacatacag gaaaataccg ctgttcataa aagctacatg
    64261 gagtgcagtc aattggtctt cgttcatgtt tactgctttt ttcatataat attcttggac
    64321 tagttctgga tattcgatca ttgcagtgaa caagtcggta aaaatgactc ctttttctgc
    64381 taattccaca ggcagttgtt caaaaatcgt ccatgaacct tgttggacaa gcacaggatt
    64441 gtctttcatt gcatcaaatg ccggaatcgt gcctgtttca ctgatggttt gtgtttcttt
    64501 cacatcaaat aacgaccagc gatggaattt cacccgatcg atatgcggca atggcagttc
    64561 atctgctttc gctagtgcgt cttgacggaa ggttctcatc caatcaggtt cgcctttact
    64621 taaagaaaaa tcatttaccg cgtcaagata atcgagccag tttttttctt tcattcttgc
    64681 tcccccttac gcttcttctt ctttatagtc gattcctaat tctttactga taccagcgta
    64741 gccttctgct tccagacgtt tagctaggtc agcattccct gtcatgacta ctcggccttc
    64801 catcatgatg tgaacgacat caggtgtgat atagttcaat aatcgttgat agtgggtgat
    64861 gatcaatgca ccgaaattgt ctccgcgcat ttcatttact cctttagcaa cgactttcaa
    64921 agcatcgata tccagacctg aatcgatttc gtctaaaatt gcaaatgtag gttctaacat
    64981 caacaattgc aggatctcat tccgtttctt ttctccgcca gaaaaacctt cgttcagata
    65041 acgttcagcc atttcttctg gcatatttag taaagccatt ttttcatcta actttttcaa
    65101 aaatgccata acagaaattt tgtcgttttc atcacgtttc gcattgatcg ctgcacgcat
    65161 gaattccgca ttcgtgatac ctggtatctc acttggatat tgcatagcta aaaataagcc
    65221 aagtcgcgca cgttcatcta cttccaagtc taaaacattt ttcccgtcaa acaaaatttc
    65281 gccttctgtt acttcatagt ttggattgcc catgatagcg gcagataagg tagattttcc
    65341 tgtgccgtta ggtcccatga tggcatggat ttctccggtt ttcattgtta ggtttacgcc
    65401 tttaaggatt tctttttcct cgatcgacac atgtagattt tttatttcta agacagacat
    65461 gcattatacc cccaaattct ctttcgttct cactcatttt agactaattg agaatgaagc
    65521 gcaatgacct tccttactaa aatcaaatat cttttattta ttattaaaca attttgattt
    65581 ctaaattaga ataggtataa aaaagatgag aaataagcca ttacagactt atttctcatc
    65641 ttttttgttc cataccttat tttgcatcag cctttactgg tttaacagag ccgccccatt
    65701 tgtctgtgat ccattcttgg acttctttgc ggtgaagcac ttcaatcagt tttttgattt
    65761 tgtcactgtc ttcatcacct ttacgagtcg caataatatt cacgtaagga gagttgtcct
    65821 tttcaagcaa tacggcatct tctaatggat tcaaccctgc gccgtaagcg aagtttgcgt
    65881 tgattgcaac taggtcacct tcatcatttt ggtacgtaga agctaaaagt gatgggtcga
    65941 tcgtatggtt gaacttcaag ttcttagggt tttcttcaat atcatcgaat gttgctgttt
    66001 ccagatctac tccatctttg actttgacta agccagcatc ttgtagaatt gtgatgatgc
    66061 gtccccagtc tgattcagaa tttgaagtaa tgattgttgc accatctttt aattcggaaa
    66121 catctttgac ttttttagag tacaagccca ttggttcgat gtggacagca ccggcattga
    66181 caaagtcata tcctttttct ttgatttctt tgttcaaata aggaatatgc tggaagtaat
    66241 tggcatcaat gtcgccttct tccaaagatt ggttcggtaa tacatagtca tcgaatttga
    66301 cgatttcaag atcgattcct tcatctttca ataatggttt cacatgttcc aaaatctctg
    66361 cgtgcggtgt cggtgaagcg ccgactttca atgtcgtgtc gtctgcttta tttgctgaat
    66421 ctttgctgtc gctgttgtta ccgcatgctg ctaatcctac agtcagcaaa agcgctgctg
    66481 caaatccaaa aattttcttt ttcatatcct acatccccct atatcctatt atcttttgtc
    66541 tgttttctta gttaagaaat caccgattcc ttgaacgata aatacgatta gtaaaataat
    66601 taaagtcgca acgaatgtca ctgttagatt accacgctgg tagccttcct gccaagcaag
    66661 acctccaagt ccacctgcgc cgatcgcgcc ggccatcgca gtaaatccga tcatcgagat
    66721 cgttgtaact gttaatccag aaatcaatgc gggaatactt tcaggtatca agaccttcca
    66781 gatgatttcc cagtaacttg ctcccattgc atctgctgct tccagtacac cagggctgac
    66841 ttcacgaaaa gcaatctcta ctagacgggc ataaaatggt gctgcggata aaacaagagc
    66901 tggaatggct gcttgcgcac ctagcatagt gcctacgatt gctttagtaa atggaatgat
    66961 caaaaccatc aggatcataa atggcgttga ccggaaaata ttactgatga tcgatacgat
    67021 tccataaatg attcgagcat acggtttgtt tttgcgtcca atagagtaaa ggatcaatcc
    67081 caaaactaat ccgatgatcg tcactaagac cattgagatt aatgtcatat atagtgtatc
    67141 aaggatagcc tgattcatcg tttccgggtt gatttgttta aaatctagat atgtttctga
    67201 aaagctttta tccacgatgg atcacctcaa ttcctactcc ttgttctttg atgaacactt
    67261 ctgcttgatc taattctttg gtttcaccag tcatcaagac agtcaatgaa ccaaaggaac
    67321 cttctttcac ttgtttgatc tttccataca caacgttgac atcgatgtta tagcgtcgaa
    67381 tcgcttggga aatgactggc tcgttggcat ttctttcgct aaaggtcaaa gttgccaaac
    67441 ggccgcttgg attttctttg atgagttcag ctaatagttc tgtcgaatca gcttctggtt
    67501 cgatgtcttg ttgtacaaag cgtttggtta cttctttttt cggttgtctg aagacagtca
    67561 atacgtctcc ttcttctacg actttcccca actccatcac agcgactttg ttgcagattt
    67621 tgcggatcac attcatttca tgtgtgatca agacgatggt caaattcaat ttcttattga
    67681 tcgctaacaa taagtctaat acttcttctg ttgtttgcgg gtccaatgcg ctagtagcct
    67741 catcacaaag caggatctca ggatcatttg ccaatgctcg tgcgataccg acgcgttgtt
    67801 tctgtccgcc ggaaagttgt gaaggatatg cttgtccgcg tccttctaac ccgactaaac
    67861 gcaacagttc ttctgcccgt tctttacgtt tgctctttgg tacgccagct aattccagcg
    67921 gcagctggat attttcaagt acagttcttg accataacag gttgaaatgc tggaagatca
    67981 taccgatttt ctttcggaat gtacgtaatt ctttgttttt taattttgta atatcttgat
    68041 cgtttacgat cacttcgccg ctcgtgggaa gctcaagtcc attcaggaga cggactaatg
    68101 tactttttcc tgcgccagaa tagccgacga ttccataaat atcccctttt tcaacgttta
    68161 gtgaaacgtc attgacggct tggatgtttc cgtttttccc attgaatgtc tttttgacat
    68221 tcgttagttg gattaatgac atgccattca catctttctc ttataatttt tcaaacaaaa
    68281 aagccttcgc cctcttgcct taagcaaaag gacgaaagct tgaacttccg tgttaccacc
    68341 ttgatttacc gttacttcgc aataacagcc tcttccagta cttggactta gcttctttcc
    68401 gtatactgtc gcgctgtaac gggcactccc gtagtaagtt tgcttacgct cgcttactct
    68461 gctctaagac catcttccat ctcattttcg ttacccattt tcagcaaaca gggctctctt
    68521 caaacgtccg tgacatgtac tcttcttttc aaagctttct cgtttcatat aggattgctt
    68581 atgattatag caactggaag aatcagtgtc aacttttttc aagtgatttt ctgattttat
    68641 aattcgtcaa attcttttgg atcaacatct ttgaaactta agatccacgc attcatttca
    68701 tctgaatcat tcaagatatt gatatcttca ccgattttat cattaacaga agaaactgta
    68761 cccgttagcg ggctggcaaa ttcattgacc gctttttccg cttcaacttc gatcagaggt
    68821 tcatcttttt ttagcgattg tcctgctttt ggcagattgg caaacgtgat gtctccaagt
    68881 tcttcttgcg cttcatttgt caaaccaaca cagtattctt ttccattaaa taagatccaa
    68941 aggttgtcct ttttcttcaa atgcttgcct gccattttat atcagttctc cttcataaat
    69001 tttttcattg aatccaattg caacaagctg gtcctctcgt aagatcagtg gtcgcttgat
    69061 cagcatacca tctgtcgata agacttggct cgcttttgaa gccgtaaact cgcctacttg
    69121 atctttcagt ccaagttccc gatatttcat cccgcttgtg ttgaacaggc gacggattgg
    69181 tacttgactc gtttctagcc aagtctcaat cgttgaggcc ttcggtactt ctttgatcat
    69241 atcaacggtt tgataactaa cgccatgctg gtctaaccac gctttggctt ttttgcatgt
    69301 tgaacatttc gggtaccagt aaaaggtgta caattttctt acctccttga agatcttgca
    69361 ggtgcttcat tttggtatct catcgataat atcaacccga taccgatcat gttccctaaa
    69421 atcgaggatc ctccctgact aatgaaaggc aatgggatcc ctgttaaagg aagtaatccg
    69481 atatttgctc caatattctc aaagacatgg aacagcagca tcatgatcaa accgcttgcg
    69541 atataggcgt aaaattcatt attcgtatcg aaacacaccc gaatcatacg atagattaaa
    69601 ataaaataca agaaaatgac gaatgcacca ccgataaagc cgaagttttc accgataacg
    69661 gtaaaaatca tatcggactc acgaacaggc acatagatat tgcttacgtt gaatccttta
    69721 ccaaacaagc caccggtacc aattgccaaa agtccttggg ccggctgata actcattcct
    69781 gacgtatcat ggaaagggtc cagccatgaa tcaatacgtg caaactgata agatttgaat
    69841 ccaactttat aaagcaagtc tcttccgcct tcagtcgtaa ccaagaagat cgttcctgct
    69901 ccaacgacta ctgccaagat tactacaggt gcgatgatgc gccaagagat tccagacatc
    69961 aggaaaatcc cgccaaagat cgcaagaaag acaagcatcg ttccaaagtc atcttgggcc
    70021 atgatcaatg ctaaaaccgg tattgtcacg atcaagagct tcccaatcag ccaaaagtcc
    70081 gatttcatcg tccgttcttt tacttgcgta ttatgtttag ttacgatcag tgccatcatc
    70141 aagatgtacg caatcttcat caactctgct ggctggaaag taaacgcacc gaatcgaaac
    70201 cagtttttag accctgttga agcagctaaa ttctgatcat agaatttgag caagagtccc
    70261 attaccacta accccaaacc ataaaggtaa ggagtcagtt tccagatcca tttggaattc
    70321 aaatgcatga tgacaacgat tgctgccgct ccaactaaat accagacaac ttgcatccct
    70381 acgcctttca agactgctgg attgttcgga tcatggctaa gtgctacata taatgagagc
    70441 agaccaatca aacataacag aaagactggt aaaatcacgc cataatcaat acgattatcg
    70501 ttcgaagctt ttaatttttt ttccatgttt catccctttt cttaaaaacc atactgctga
    70561 ttattatagc ggtccagtta gataatggaa agtctttcca tagacatttg ggaaaaatta
    70621 actttccgtt tgtgcagttg cttgagcaaa tgcagtacaa aagcctactg cttatcttta
    70681 gtgtatcata gaactttttt tgatacatgc aattatctac cagctgagat tctcctcgct
    70741 gtgagaagtt tctgtgcttt cattgtatcg aaggatctct tcggcaatct ctagcagctg
    70801 atcagaaacc agattggttc gttggatgaa ataaaaatat cgatggaaat tcaacggagt
    70861 cattggcaca ttttctggaa cagagcgctt agacaaaatc gagcgaccga ttccttcctt
    70921 cagcaacgca acaattacat cgttactttt cacgatcatc ttcttatctt ggatattcat
    70981 ctcttcaaaa taacgctgcg tgtaatgaaa aacaccagag tttgcttctc ttacaagcca
    71041 caatttgctt tttgggtccc cagcgatgac tagctggtct tccatcaaag agtatctttg
    71101 tacgccggaa gtctctaatg gcttttcgat aaaaccaaaa tcggattcat gtttcgctac
    71161 ttgttctaat gattcatgag aattcgtgat ttcgatctca aaatgcactt taggaaacct
    71221 tttgatcaga tgtttcatca actgaggcaa aagatagaca gcaaaggtgt gggaagcaac
    71281 gatccgacat acttcttctt cattttgttc attgagcaat ttttgctgag tttcttccca
    71341 ttcttctaac aaaaccagtg tccgttcata taataagtcg gcttgcttag ttggaagcat
    71401 ctcttttctt ccgttacgtt gaaaaagatc gatattcaat tcccgctcca actgtttgat
    71461 ttgattcgag actgctggtt gagataaaaa cagtacttct gctgctctgg aaaaatttct
    71521 tgtttcatat actgctcgaa aagtcgttaa taacttaaac atggcaccac ttccataact
    71581 ttttatgata attactataa aaactatttg atttaaatat acaaaaaacc atctataata
    71641 gaaatgtaca acaaaacagt tgctgatcca actagtaact tagatgaaga caatcagctg
    71701 tctgcttgct cgtaatcata cattcctatt cattttacaa tgtattcgtt catcttggta
    71761 ctaaaatcta ttaaataaaa aggagaatat tatatatgaa gaactttttg aaaattattc
    71821 cgatcccgat ctgcgggttg atccttgggt tagcttccct tggaaatctg tttaaagact
    71881 atcaccatgt tgctttaggc aatctaactg gcggtatctc tatgattctt atgatattca
    71941 tttttgttaa attgtttatc ttattcgaac acacgaaaca aacgttgcat gatccaatca
    72001 tcgcctccgt ttcaccaact tttacgatgt cactcatggt tatttgtact tattttgttt
    72061 catatgaaag tattgcacca attgtcaaat tcatttggct gtgcgctgtt atcttccaag
    72121 tgatccttgt cttttatttc aattatcacc atgttatcaa agctgatctt tcaattgaag
    72181 caatttatcc aagctggttt atcatttacg ttggatttgg ggtcatcact gtaactgccg
    72241 ggaacttctt cccacttatt ggaaaaattt tcttttggat ttcattgatc tgctatgcgt
    72301 tcttattgcc gatcatcatc aaacgtgtct ttttcgtaag aaatatggct catccgacac
    72361 gtccgctgat tacgatcgtt gcagcacctg gttctctttg cctaacaggt tatcttaaaa
    72421 actttgctga acctaatttt tatcttgtcg tagcattgtt cgctttatct caagtattgt
    72481 atttcgttgt gctgtctatg cttccacgtt tgttcaagct tcgtttttat ccaagctatg
    72541 cagcctttac attcccgctt gttatctctg caacagctat atttactact gttcattact
    72601 tcaacagttt aggtatctat agtagcgtat tggatttctt aaaagttgcg gaatgtctgt
    72661 ttgcaagtgt agttgtcttc tacgttcttg ctcattacgt acgtttctta attaaagaac
    72721 atagcaaaca tggaaaattt gtttctaaaa aaatcgctta ataatcgatt caataggttg
    72781 tgaaaaagct gttctgcatc aagtaataag aacggccaaa caaaaacagc tgttcatgtt
    72841 tttgtactgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
    72901 tcgtaa
//
LOCUS       DACEOM010000002        72182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEOM010000002 DACEOM010000000
VERSION     DACEOM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.002"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(507..1061)
                     /locus_tag="IW781_000080"
     CDS             complement(507..1061)
                     /locus_tag="IW781_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1181107.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1187..3116)
                     /locus_tag="IW781_000081"
                     /pseudo
     CDS             complement(1187..3116)
                     /locus_tag="IW781_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3422..4138)
                     /locus_tag="IW781_000082"
     CDS             complement(3422..4138)
                     /locus_tag="IW781_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1181108.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4139..5962)
                     /gene="glpO"
                     /locus_tag="IW781_000083"
     CDS             complement(4139..5962)
                     /gene="glpO"
                     /locus_tag="IW781_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1181109.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5976..7472)
                     /gene="glpK"
                     /locus_tag="IW781_000084"
     CDS             complement(5976..7472)
                     /gene="glpK"
                     /locus_tag="IW781_000084"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1181110.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7608..9068)
                     /locus_tag="IW781_000085"
     CDS             complement(7608..9068)
                     /locus_tag="IW781_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1181111.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9058..10353)
                     /locus_tag="IW781_000086"
     CDS             complement(9058..10353)
                     /locus_tag="IW781_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1181112.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10685..11362)
                     /locus_tag="IW781_000087"
     CDS             complement(10685..11362)
                     /locus_tag="IW781_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1181113.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11392..12483)
                     /locus_tag="IW781_000088"
     CDS             complement(11392..12483)
                     /locus_tag="IW781_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1181114.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12584..13018)
                     /locus_tag="IW781_000089"
     CDS             complement(12584..13018)
                     /locus_tag="IW781_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1181115.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13602..15059)
                     /gene="ascB"
                     /locus_tag="IW781_000090"
     CDS             complement(13602..15059)
                     /gene="ascB"
                     /locus_tag="IW781_000090"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1181116.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15056..16951)
                     /locus_tag="IW781_000091"
     CDS             complement(15056..16951)
                     /locus_tag="IW781_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1181117.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17009..18784)
                     /locus_tag="IW781_000092"
     CDS             complement(17009..18784)
                     /locus_tag="IW781_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1181118.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18834..20330)
                     /locus_tag="IW781_000093"
     CDS             complement(18834..20330)
                     /locus_tag="IW781_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1181119.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20524..22146)
                     /locus_tag="IW781_000094"
     CDS             complement(20524..22146)
                     /locus_tag="IW781_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1181120.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22474..23787)
                     /locus_tag="IW781_000095"
     CDS             complement(22474..23787)
                     /locus_tag="IW781_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1181121.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24032..24256)
                     /locus_tag="IW781_000096"
     CDS             complement(24032..24256)
                     /locus_tag="IW781_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181122.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24276..24644)
                     /locus_tag="IW781_000097"
     CDS             complement(24276..24644)
                     /locus_tag="IW781_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1181123.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24840..25550)
                     /locus_tag="IW781_000098"
     CDS             complement(24840..25550)
                     /locus_tag="IW781_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181124.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25568..26065)
                     /locus_tag="IW781_000099"
     CDS             complement(25568..26065)
                     /locus_tag="IW781_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1181125.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(26102..26554)
                     /locus_tag="IW781_000100"
     CDS             complement(26102..26554)
                     /locus_tag="IW781_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1181126.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26689..27294)
                     /locus_tag="IW781_000101"
     CDS             complement(26689..27294)
                     /locus_tag="IW781_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1181127.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27744..28364)
                     /locus_tag="IW781_000102"
     CDS             complement(27744..28364)
                     /locus_tag="IW781_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1181128.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28366..29235)
                     /locus_tag="IW781_000103"
     CDS             complement(28366..29235)
                     /locus_tag="IW781_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1181129.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29258..29665)
                     /locus_tag="IW781_000104"
     CDS             complement(29258..29665)
                     /locus_tag="IW781_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1181130.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29684..30973)
                     /locus_tag="IW781_000105"
     CDS             complement(29684..30973)
                     /locus_tag="IW781_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1181131.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(31000..31302)
                     /locus_tag="IW781_000106"
     CDS             complement(31000..31302)
                     /locus_tag="IW781_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1181132.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31327..33420)
                     /locus_tag="IW781_000107"
     CDS             complement(31327..33420)
                     /locus_tag="IW781_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1181133.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(33909..34673)
                     /locus_tag="IW781_000108"
     CDS             complement(33909..34673)
                     /locus_tag="IW781_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1181134.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34784..35653)
                     /gene="mdcB"
                     /locus_tag="IW781_000109"
     CDS             complement(34784..35653)
                     /gene="mdcB"
                     /locus_tag="IW781_000109"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1181135.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(35678..36754)
                     /locus_tag="IW781_000110"
     CDS             complement(35678..36754)
                     /locus_tag="IW781_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1181136.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(36778..37371)
                     /locus_tag="IW781_000111"
     CDS             complement(36778..37371)
                     /locus_tag="IW781_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1181137.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(37375..39051)
                     /gene="mdcD"
                     /locus_tag="IW781_000112"
     CDS             complement(37375..39051)
                     /gene="mdcD"
                     /locus_tag="IW781_000112"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1181138.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(39041..39340)
                     /locus_tag="IW781_000113"
     CDS             complement(39041..39340)
                     /locus_tag="IW781_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1181139.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(39442..41088)
                     /gene="mdcA"
                     /locus_tag="IW781_000114"
     CDS             complement(39442..41088)
                     /gene="mdcA"
                     /locus_tag="IW781_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1181140.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDAFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(41095..42006)
                     /locus_tag="IW781_000115"
     CDS             complement(41095..42006)
                     /locus_tag="IW781_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1181141.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            42126..42983
                     /locus_tag="IW781_000116"
     CDS             42126..42983
                     /locus_tag="IW781_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1181142.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(43170..43532)
                     /locus_tag="IW781_000117"
     CDS             complement(43170..43532)
                     /locus_tag="IW781_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181143.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            43878..45317
                     /locus_tag="IW781_000118"
     CDS             43878..45317
                     /locus_tag="IW781_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1181144.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(45324..47876)
                     /locus_tag="IW781_000119"
     CDS             complement(45324..47876)
                     /locus_tag="IW781_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1181145.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(47990..49321)
                     /locus_tag="IW781_000120"
     CDS             complement(47990..49321)
                     /locus_tag="IW781_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1181146.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(49287..50765)
                     /locus_tag="IW781_000121"
     CDS             complement(49287..50765)
                     /locus_tag="IW781_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1181147.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(51070..52905)
                     /gene="tdc"
                     /locus_tag="IW781_000122"
     CDS             complement(51070..52905)
                     /gene="tdc"
                     /locus_tag="IW781_000122"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1181148.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            53375..54802
                     /locus_tag="IW781_000123"
     CDS             53375..54802
                     /locus_tag="IW781_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1181149.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(54894..55259)
                     /locus_tag="IW781_000124"
     CDS             complement(54894..55259)
                     /locus_tag="IW781_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1181150.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(55256..55621)
                     /locus_tag="IW781_000125"
     CDS             complement(55256..55621)
                     /locus_tag="IW781_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1181151.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(55813..56061)
                     /locus_tag="IW781_000126"
     CDS             complement(55813..56061)
                     /locus_tag="IW781_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181152.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            56060..56188
                     /locus_tag="IW781_000127"
     CDS             56060..56188
                     /locus_tag="IW781_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1181153.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            56245..57939
                     /locus_tag="IW781_000128"
     CDS             56245..57939
                     /locus_tag="IW781_000128"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1181154.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            57943..58062
                     /locus_tag="IW781_000129"
                     /pseudo
     CDS             57943..58062
                     /locus_tag="IW781_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            58144..58335
                     /locus_tag="IW781_000130"
     CDS             58144..58335
                     /locus_tag="IW781_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181155.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            58713..59846
                     /locus_tag="IW781_000131"
     CDS             58713..59846
                     /locus_tag="IW781_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1181156.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            59877..60197
                     /locus_tag="IW781_000132"
     CDS             59877..60197
                     /locus_tag="IW781_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181157.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(60226..60690)
                     /locus_tag="IW781_000133"
     CDS             complement(60226..60690)
                     /locus_tag="IW781_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1181158.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(60821..62078)
                     /locus_tag="IW781_000134"
                     /pseudo
     CDS             complement(60821..62078)
                     /locus_tag="IW781_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(62107..62424)
                     /locus_tag="IW781_000135"
     CDS             complement(62107..62424)
                     /locus_tag="IW781_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1181159.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(62450..62770)
                     /locus_tag="IW781_000136"
     CDS             complement(62450..62770)
                     /locus_tag="IW781_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1181160.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(63234..64649)
                     /locus_tag="IW781_000137"
     CDS             complement(63234..64649)
                     /locus_tag="IW781_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1181161.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(64666..65982)
                     /locus_tag="IW781_000138"
     CDS             complement(64666..65982)
                     /locus_tag="IW781_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1181162.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(66132..66830)
                     /locus_tag="IW781_000139"
     CDS             complement(66132..66830)
                     /locus_tag="IW781_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1181163.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(67119..68108)
                     /locus_tag="IW781_000140"
     CDS             complement(67119..68108)
                     /locus_tag="IW781_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ1181164.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(68188..69021)
                     /gene="msrA"
                     /locus_tag="IW781_000141"
     CDS             complement(68188..69021)
                     /gene="msrA"
                     /locus_tag="IW781_000141"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1181165.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(69196..70830)
                     /locus_tag="IW781_000142"
     CDS             complement(69196..70830)
                     /locus_tag="IW781_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181166.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(70817..72151)
                     /locus_tag="IW781_000143"
     CDS             complement(70817..72151)
                     /locus_tag="IW781_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181167.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        21300 a        15184 c        12086 g        23612 t
ORIGIN      
        1 cagcggtttg acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg
       61 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaaggaag agattgtgaa
      121 aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggatttc tacatttttg
      181 cataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttaagg aagaggttgt
      241 aaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagct gctcatattt
      301 tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg
      361 tgagcctacc atttaactcc gaatcacaag aaataatttc aaaaacaact tctcctgttt
      421 ttggttcggt tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg
      481 aatgcgacat aggtcacacc ccttttttat tttctttgaa acatctttcg gtactcgaca
      541 gcatacaaga caaaagattg taaaagaccg taagtgatga cggcaaaaat gactctcacc
      601 cacagcgttg gtaattctgg gaaaagcagc aaaaggatca tgccaacata gatgacagca
      661 gtcgctactt tgccatacca cttcgctcca cttaatttga tatttttaga tagcccgatt
      721 aatccagcta caaacataaa gccttctttt atcaagaata gaccaaacaa cagccaaagc
      781 cacggtcgat accatgccat agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac
      841 ggatcaagca ccttccctaa gtctgttacc tgattatatc tccgagcaat gaccccgtct
      901 agataatcag acaaacctga gacaacaatg accgctcctg ctatataaaa tgactgtctc
      961 agcacaaaat aaagataaat cggcagcaaa aaaatacgga atatcgataa aagattcgga
     1021 atcgtttgta tttctttacg ccagtctgtg cttaatttca ttttatcgcc ctacctcctc
     1081 agcactcttt tctttttatt ttagcttaca cggggaataa aaccaatgat ttcactgttt
     1141 ttatttttct ttacagacac tgatattatg agctaatttg agataatcac ttgctgtttt
     1201 catcgtttac tcttctttct tcctgtcgtt tgcgcatttc ttttactagt actttatgag
     1261 ctttttttga tcctcgctct aggtcacgct gccgtttacg tgttttataa tcgataaaca
     1321 gactgtctaa atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc agacgaacac
     1381 gctgacgagg cacgtggatt atttttttgt ctaaatatac atcaatcgta tcttcaccta
     1441 tatcttcata aactaacgct tctttttggt attcattgac aaatatccga tcaccttttg
     1501 caaagagctg acgttctttt ttattctcct tcctatttgt aactgctgtc ttaggaagaa
     1561 attctttttt ctgtgtccga taggcttttt ccgccaagta acgctctgct tgatggatca
     1621 atttcatcga catatccatt ttatgagcaa tccacaaagc ttggctttct cctgtttctt
     1681 ccaacccgca actgatattt aggacgaagt gcttcccgat cgaaagccat agcagccggc
     1741 acaaaatctt catgatctcc ggcgaatttc ttgatttctc cataatgggt cgtggcaatg
     1801 atcaatgccc cttgctcata catagattcc atgattgcga tcgctagtgc agccccttca
     1861 tttggctctg ttccactgcc aatctcatct aaaagtacca acgtattcct ttttatcttt
     1921 ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc attttctaaa
     1981 ttttggtggt caccgacatc gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct
     2041 tttgccggaa cttgcatacc gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac
     2101 acgactgttt tgcctccggc atttgctccg gtgataatca agcctcgata gtctttaccg
     2161 atttcaacat ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata
     2221 atgatatgtt ccgatttatt gattctaggt gtgcttccat tgatttctcg gctaaatttt
     2281 gcccgagcaa agatgatatc taacaccgtg atcgtttcga tcaataaatc gatcacttct
     2341 tcgttttctg cgatagcacc agtcaatgcc gccaggactt gatattcttc actgatttct
     2401 tcagctttta acaactgata gtgttcattc aatttagaaa tcacagttgg ttcaataaaa
     2461 accgtcgttc ttttatttga ttcatcgata atcgttccat ctattttatt tttatagctg
     2521 gctttgatag gaatcgtata gtattcgcct ttttgaacaa tcattgcttc ttggatcatt
     2581 tctttgtttt tcggatgacg caagaatttc agtaattttg actggatttc tttttcgatt
     2641 agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact gactttatta
     2701 ttttttattt tctggtcaat caactcttct acatttatca aatcaggaag atgtttgcta
     2761 taagcaaaca acaagggtgt ctggtattga ttcttatcaa aaaactttgt gatcatccga
     2821 ctgcttctaa gaaaatccgc atattcgatc aaatcggttg gctgcagaat caaccctttt
     2881 ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca taaatggaac atgctgactg
     2941 ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac agtttgtagg
     3001 tttgtcgaga ccgtcatatc gctgattcgt tttttgctat agtttccaat tgctcttgct
     3061 tggatttcct ttttgatccg atcaaattgt aattgactaa tcatttcgtt gtccatattc
     3121 tctccttttc ttgaagagat acctgtccca aaataaataa gggcctagag ataaataaaa
     3181 ggatagaaca cagcttaaaa aagtcatgtc ctatccttat tagaatacaa agatattgtt
     3241 ttcctgacag gtaccgtttc ataagcacac caaataaact ttgtttattg gctattcgac
     3301 ttactgatta gataccagct acactcacaa aacatacccc attcttctag gtcttattta
     3361 ttttccactt aactatagca agacacattt tggttgtcaa actttagtat gttttataga
     3421 tttacaccat taatgaatag acacctaccg cgataattgc tccgatcatt ggacctgcga
     3481 ttggtaccca tgaatatgcc caatctgaag ttcctttatt agctattggc aataccgcat
     3541 gtgctaatcg aggaccaaag tcccgagcag ggttgattgc atatcctgtt ggccctccta
     3601 atgaaagacc gatggctaag atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa
     3661 attcgttttg tccaaaagct agcaacccaa gaactaatac aaatgtacca attaattctg
     3721 tgatcacatt tgctggataa ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg
     3781 cgctttcatc tttcgttgca ttccaatgtg gcaaataaga aagccaaaca agaatggccc
     3841 ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac gacaagattc caactaattg
     3901 cccctgtcat cgccatagct agagagactg caggattcaa atgagctggg cccatgaatc
     3961 cagaaatata gacagccatt gttacagcga gtccccaacc aaatgcgatg acaatccagc
     4021 cagaagcctg tgctttactt ttgtttaaat tcacagccgc acacacacca tctccaagca
     4081 aaactaggat cagtgtccca ataaactccc ccatgatttg agtcatatca ctgttcattt
     4141 atttctcccc ctttagttcc cgcagatctg attctgcaat caatgcttct aaacgtttag
     4201 tctgttgcgc cttctcctct tctgtccatt caaaatatcc agcaattgca tcgatcacag
     4261 gttgcttgat ctcatcaagc tgatctcgtt caaacaacag atgatttgta cgacgaatga
     4321 gataatcgcc tggggccaaa accatttctt cttctaaacc ataacgtaac cgagctgact
     4381 cagcgagact caaaccagga taaggtgcca tttcttttgc taattcaaag atacgtcggg
     4441 cattagtccc gtaaaaatca gcgatataag tagcatcttc ttcctctaaa ccggctgcta
     4501 ctccttcttt tgctaattct gtcactgttt cttctaattt agttggatcg aagtttccac
     4561 ctgaaatctg atatttttta gagtcgattt ctttcgtctc tattccgtat tcttctttta
     4621 ataattggcg tatcaatcgc aaagctcctt ctgccatctt acggtaatcg gtgattttcc
     4681 caccagataa agtcagcaaa ccatctggtt ctctttctaa agaactacct ctggaaatcg
     4741 tagaaggtgc tttttcatca cggctgtttt ccaaatggtt caacacgtct tctacttcag
     4801 cacgagaaac tttgttttcc ttatattcac ttactgtatc gacaacttta ttgaagctct
     4861 tgtctgaaat cgatccatta tctccaccat tgtaatcaga accagaatta ccaatcaaca
     4921 gtggacgaag tcctgcccag cttgcttcga tatcattcaa tgtgatattt gcttctggat
     4981 agcgatgatt gatcacatcc aatagataat ctacatcttc ttgtgtgact ttcggatccg
     5041 taaaatctcc ttggtaatcc gtatctgtcg taccaaagta agtcttgttt tctcttggaa
     5101 tagcaaaaac catccgccca tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag
     5161 gcagtttttt cgcatctaca actaaatgga tccctttagt tggacgcatt ttaggagaga
     5221 ctggacgcgt aaaattcaag tttcgtactt tatctaccca aggaccactc gtattgatca
     5281 ctaattttgc tttgatttcg atcacttcat ctgtcagcag gtcacgagct ttgacgccaa
     5341 cgatttgatc gccatcatat aaaaagccaa ccgctttcat tttacttact agataagccc
     5401 catcttcggc agcctttttg atattatcaa tcactaaacg ggcatcattg ttgcggaaat
     5461 ctagatagac acccgcgcct tttagccctt cttttttcaa aaacggttct ctttccaata
     5521 cttcttctgg tgtcagcgta taattctcat atttggttcc tgtcacattt gccagtttgt
     5581 cataaaggtc cattgctact ttgaccgaga acatattgaa ggttgttgct ccttcgccct
     5641 catagattgg caaaagcatt ggatctggtt ttgggatgtg tggggcaata ctttgtacga
     5701 ctgcacgttc accaactgtg tcagctacta cttccacatc aaatgtcttc agataacgaa
     5761 taccgccatg aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt
     5821 cgatcaatcc tgttttgatt cctgatgctg ctgactggac tgccacaccg gcaccagtga
     5881 ttccgccacc gatgatcaac agatccaatt cttcttgctg cattttttga atgctatctt
     5941 gtcttgtctt gtttgaaaac atatggattt cctccttatt caaatgcttg ggtagctttt
     6001 actgcttttt tccaaccgtt gtaaagtttt tctcggcgct cttcctccat ttgaacttca
     6061 aatagcttgc cagcttcata aaattcgcgg atctcgtctg tatctttcca ataaccgaca
     6121 gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc tgctcgttgg
     6181 atcggtacat tcaagatatc tgtttggaat tgcatcagat attcattgtt tgctgcaccg
     6241 ccatctactt tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt atcgatgata
     6301 tctctcactt gataagcaat cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta
     6361 ccacgtgtca agccaaacat agcccctcgt gctgcctgat cccaataagg agcacctagc
     6421 ccaacgaatg caggaacaac ataaacctca tcttcgcttg tagagcgttt cgctgcgtct
     6481 tctgaatcgc tagctttttg caacatttga agtccatctc tcagccattg aatcgaagaa
     6541 ccagccacaa aaatacttcc ttctaaagca tagtaaacct ttccatttat gccgtaacca
     6601 attgtcgtca ataaattatt tttggataat tgcggttttt caccggtatt catgacaata
     6661 aatgaacctg taccataagt attcttgacc atgcctggtt cgaatgccat ttgtccgaat
     6721 aatgcggctt gctggtctcc tgccatccct gaaattggtg cttcgcttcc ataaaaatga
     6781 taaccttgcg tataaccata aacttctgag ttagaagtga cttttggtaa catcactcga
     6841 ggaatattta gtaaatctaa aatctcctga tcccaatcta gctgatggat attgaacaac
     6901 atcgtccgac tggcattcga gtaatcagta acatgtgctt gtccatcggt cagtttccat
     6961 actaaccaag aatcgatcgt tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg
     7021 ccttctacat gatcaagaat ccagcgtatc ttcgttgctg agaaataggc atcgatcact
     7081 aaccctgttt tttcgtggaa aaattcttga tagccttcat tttttaagtt atctgcaatt
     7141 ccagctgact gtctggattg ccaaacaact gcatgataga ttggacgtcc tgtatctttt
     7201 tcccatatta ctgttgtttc acgctggttc gttatgccaa tcccagcaat ctgatttggt
     7261 ttgatccctg attcgatgaa cgctccggca ataactgact gtacagaatt ccagatttca
     7321 tttgcatcat gttccaccca actttcttgc gggaaatatt gcgtgaactc tttttgagaa
     7381 ctgccgatgt gtcttccttt cttgtcaaag ataattgctc gtgagctagt tgtaccttgg
     7441 tcaatcgcca tgatatacgt tgattctgtc attgtgattc cctccattat atgcaaacgc
     7501 tttctttaac tactcttatc atactactgt tttaaaaaaa gaaactgtca aaattttatt
     7561 ctttttttct ttttttgaca gttttttatt atgaagctat tttttaatca attcttttct
     7621 tttttcagcg tctctattct tcttttcaat cgaataatgt cgtaaggtga cgcaaattct
     7681 gacagataat attcacactg ttttttgttt ctctggcaaa atgtgatcac caaatcatat
     7741 tctttacctt cttcataact tccaatctca ataccgccga ttcccctcag ttctctgatc
     7801 agatattgtt gaaaagcaat acgataaatc ggcagacttt ctaaatcgat tccaatcact
     7861 actggttttg caatataaaa atcgatcatc aaaagaacat tggcatactg gatcatcagg
     7921 taactaagct cactatcttc agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt
     7981 aatttcgcca gtaactgtgt taaggaatgc ccgagcattt tctgctgctg ttccaaaagc
     8041 cgatcacgct catagacttc gatctttcca acaaagaaaa accattcatt gttgatctgg
     8101 gaaatttggt agcctactgc cttttcttct ttgattttca aacgacgagg acgataatgc
     8161 cataacatcg tttcccgaat gtaagtatct aaaacagcgg ttggtaactt tttgctccgt
     8221 gtcaaatcat actgataaaa tatttcttcg tctactatcg aaaaagaaac aaaaaagctg
     8281 taaaacatca ttgtttcgta tacattcagt tctgctgctg ttcgactaag atgcatcacg
     8341 atgattggat cgattgcttg ataaagccgg tccgactgat agagtaattt cttttctttc
     8401 aaagttgcgt acgtactttt ttcattcaat agtctttttt tcgtaatccc gagccagcaa
     8461 gcaatctgtg cttctgctga tgccgtaaac gttgtttcta atacacggtc caatcctaga
     8521 atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata
     8581 tattggaata gttcataata aaaatatcgt atctgtagct cttctccgta taattgaccg
     8641 tttttgattt gtaatccaaa ttcctctaag agctggttca attctttgat ctttcgaaaa
     8701 acagaagact cactgatatt aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt
     8761 tctaataaat agacgagcat cttaaacttc aagctttcct gcaataaaaa caatagaatt
     8821 ttttccaaac ttacatcttc gtccaacaca agcagcgcct gatactcatt tcgctgtact
     8881 tctacttttt tttccatcat tcggccaatc aattcaatat cttccaaata ttgatcaaac
     8941 gaggcttttg acagttggac atgctctctc aaaccattga tagaggctgt tcccccttgc
     9001 agaacaaggt ttcgcaaaat caggatctgt aactgctctt ttctttctaa taatgcttca
     9061 atcttcatag gcagtattcg ccacctttga ccacaccata tctggcacac ggtattctgg
     9121 ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc catccttctt ctactgcaat
     9181 tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt tggagctgtg ctccaagaat
     9241 ctttttttca gaatcaaata gaaatttgag tttaactttg ctttgattta ttgctgtaag
     9301 cttgtagtct acgtatcctg tttcaatttt ttggggataa aaaatgctct cttcttctgt
     9361 caaacctacg cttcctaaga aatagccaaa ccactgattg ccgactggac gataggttcc
     9421 ctgatccttt gtctgtgagc cactaattgt cttgctgaca tttcgtgctg tacggacagc
     9481 gttatttact agagaaacat acattttttc atttgttgga cgaaaagtta cttggatcgc
     9541 atcgccaatc gcataaacgt ctttttgaga cgtttgcaag tagtcatcta cccagatcgt
     9601 tccatcatca ttcaatgtta attgttcttc ccacatctga ttgtcgggac gagaatgatt
     9661 agccagcaaa acaagatcat agttctcctt tcttttttcc gtctctacaa tagcggtttc
     9721 attttcggtc aaagacacaa cttgttcttc taaaaaaagt gaaagccctt gatttttcat
     9781 ttcctttact aacggttctg tcatttcagg atctagataa cgaaaaagaa ttgactttcg
     9841 gctttcgtaa agatggattt gtttttttct tgatgccaac ccttcagcta tttcgattcc
     9901 tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc atgatctttt gctgaagagc
     9961 cgttacgttt tgtatctttt taactgggtg aataagcgtt gattctgcag aaatatttcg
    10021 aaacttttga tttgaaccag tcgctagaat caaacgatca aaatccaatt tttttcctcc
    10081 tgctaaaacg actttttttt ctttcaatcc tattactgtc ttttctgtgt aaacatggat
    10141 tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa agttgttttt cactggtaac
    10201 ctttcctttt aatatcaaat tgaacccgct gggaatccat cctaacttcg attgcttttc
    10261 aatcaaatat atttcacttg ttggcatcaa ttggcgaaga taaatagccg catgaatccc
    10321 tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt cctctttctt ataaggtgtt
    10381 ctactgctaa cttctcaatc agaaaacaca gtattcataa ctcgctttca accttatgat
    10441 acatagcgat gcagcagaat caaccctctc catcataccg tgttctaggt ctgcttctca
    10501 ataatgtact agtggaccta attgaagagt atcatttctg aagagatgtt tcaaaaaatg
    10561 tactcttata aaagaaaaaa actcgccttt tacgtacctc tgtccaaata ggaaacagaa
    10621 aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag ttagaataga cacatcattt
    10681 atttttaatt ctttaatgct tcaacaactg cttcgcctaa ttgatgagca gattgaggat
    10741 tttgccctgt aagaagccgc ccatcaattt gaaccacttc tgaaaaagct gctccagact
    10801 gatagtttgc tcctttttct ttcaaagcat cttctgctaa gaatggtact tcattcgttg
    10861 taccatttgc ttcctcttct tcattactga acccagtcac tgtacgtcca gctaaaatag
    10921 attttccaga ctcatctgta actggcaaca atcctacaac accgtgacaa acagctgaga
    10981 ccagcccttc atttcgatag atttgtgaag cgactttccc aagatctatt gcttctggaa
    11041 aatcccacat tgttccgtgg ccaccagcat aataaaccgc tgcataatca tttgcatcca
    11101 catcatcagg acgtaaggta tttcccaagg cccgatttct gaaatcttca tcttcataat
    11161 aggcccaatc gatttcatcc atattcaaaa cactttgcgg ttctaaaggt acatatccgc
    11221 ctttaggact gacaaaatct gcatcgatcc cattttttac taatacatca taaaaatgag
    11281 tcagttcact taaccacaaa cctgtaggac gctcgatctt ttcgtacttt tctacatttg
    11341 ttaatacgat caatactttc attttcccat cctctttcta taaaaattgc gttattgata
    11401 acgtgctttc aagaaattat ataatgggct tggcaaataa agctctttat atgcttcttc
    11461 aaaaggtttc gttacaaatt catcctcacg gttttccaat aatttctctg cccagtcagg
    11521 atcgacgatc atttgctgtc caattgctgc aagttctgca tatttcaata cctgctctac
    11581 atcttcgcga ttgcggatcc caccgacacc aactaaaggg atctttcctt gaatcgtgtt
    11641 atggaccact tcaagaatcg atttatcgtt gtatttatca gaacgagcta ttcgatcata
    11701 catatttaat gaaacatgca gataatctag ttttgactca cgtaatcgtt caagaagcca
    11761 gatcgtgtct tcaaaacgga tacctggtgt ttcatattct tctggtgaga aacgataccc
    11821 tacgatgaat ggttttttag catattgttc aaccgctgag aaaaccgttg ttaataattc
    11881 ttcaataaaa cgataacgat tttccaagct accgccccat tgatcctctc gtctattcga
    11941 atgcggagag aaaaattgtt ggatcaaata cgtatttgct ccatgcaact cgattccatc
    12001 aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat gcttggattg tttcaacgat
    12061 ttcttcttca ctcattgctc gtggtgtttc cgcatcagga cgttttgctg caactgcact
    12121 tgcagataca ggctgttctc cagataaagt tgctcgactt gtcattcgtc caccgtggaa
    12181 aatctgaata atcgcttttg cgccttctcc ttggattgct ttcgctagct cacttaatcg
    12241 aggaatcatt tcatcatgag cgatgctgag ttcacctggc cagccttttc cgatatcttg
    12301 gacatttgca gcaccagtaa taacagcacc aagaccttta gaacgttgtt tataataagt
    12361 gatctcatct tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca taggcgacag
    12421 catcaatcgg ttcttcaatt gaactcctga aggtaaagta attgattgat caaattgtga
    12481 cattgtcatt cctcccataa ttcgctctga ttaacctatc gagaatcatc tatcattaaa
    12541 ccactcgatt agttgtacat gcaactaatt tgcttaaaaa attttatttt ttttgtaact
    12601 tatctgttgc atcattcatt gtctgggcta attctttact tgcttcattt cccaaaactg
    12661 ctttgtatga ctgatgaagt tcttcccagc taccatcgac ttccttagca aattggcgtc
    12721 cttgttctgt aagataaact aatgacaagc gaccttcgct aactgttttg acgtatcctt
    12781 tatattctaa tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg
    12841 caatatcttt ttgtggtatt ccatcttttt cttttaatat caataaaata aaaccataag
    12901 ttggtgccag acctgtcctt ctaaacgctt catcagctaa tttattcaac atgcgatcta
    12961 gctttttcac gctaaagaaa agacattcat ctaacgcttt ttcaattggc ttttccattt
    13021 catcccctcc attggcgtac atatcatagt atgattttta ttttttgtca aaaaacacgg
    13081 aataatgtcc gcctcctctg tctagatttg aaaaaaagaa gtagccatct tcaaagaatt
    13141 tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt
    13201 cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
    13261 atttggctta ataccgaaaa actggctttt gaacaccgtt tatatcagag gttgtgaaaa
    13321 agccgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttgtcg
    13381 ttttttgagc taatgccgat ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc
    13441 ctgccgttga actccgagtc acaaggagca atttcaaaaa acagcttctc atgttttatg
    13501 actatggtga cttgtgatcg aggagttggc aggagaatac tgtttattcg taaagaaggg
    13561 gctgtgacaa gacttttgtc acagtccctt atttcctttt attataggat ttcgccattt
    13621 gattggatga cttgtctgta ccaatcaaat gattttttct ttaaccgttt ctttgtgcca
    13681 ttcccttcgt catcttgatc aacatagata tatccataac gttttgacat ttgtgaagta
    13741 gaagcgctga cgatatcaat gcagccccac gacgtataac caaataaatc tactccatct
    13801 ttgatagctt ccttcatctg ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct
    13861 tgaacggttc catcttcttc tactttgtct atagctccga gtccgttttc agctatgaac
    13921 agtggttttt gatacctatc atataattga ttcaaagcaa ttctcaaacc cttaggatca
    13981 atctgccagc cccattcact tgatggaaga tacggatttt taattcctcc agttattaag
    14041 tttccttgga ccccttccca attttctgga tgagcactga ctgcactaga catgtagtag
    14101 ctaaaagaaa caaaatcaac agggtaggct gctaagattt cttcatcacc tacttctatt
    14161 tttaagttga cacctttttc ttcaaacatt cgttgcgtat acgctggata atatcctctt
    14221 gcttgaacat cactaaagaa caaagaagtt ctttgctgct caattgtttt ttgaatatca
    14281 tccggatcac aagaatacgg aacaagaagc tgatagctga tcatacaacc gacttgtgaa
    14341 cctttgataa tttcatgaca agcttttgta gctaaagcac ttgctataaa ctgatggtgt
    14401 gcaccttggt atttcgcttg ttccaaatgg ggatggtttt cttcaattac tcctacgcta
    14461 acaaaagggt gatgttttac acagttgatc tcattgaaag tcatccaata ttttacttta
    14521 tttttatagc gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat aagctttcga
    14581 ttaggccatc caccatattc tgtaaccaaa tataacggca tttcataatg ggaaagagaa
    14641 acgactggct caatcccata ttttagacat tcgtcaaaca cttgatcata aaaaaataat
    14701 cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac
    14761 atccgatagc atttaaaacc catttcagca aataaacgga tgtcttcttt aaagtgatga
    14821 tagaaatcat ttccgtgtct tttaggataa taatacttgc tgttgggatc ctgcgccgct
    14881 tttacttgct gatgcgtcat catatgccat tgatctgtcc atttttcttt aggtaaatcg
    14941 ctgtcgaatg tatacacatc agacacagat aatccttttt catctacatc ccatgcacct
    15001 tcacattgat tggccgctgt cgctccgccc cataaaaatc cttctggaaa ttttttcata
    15061 tcgatccccc taaacttgct agttttcttg tttgattatt tttcttatga ctagtaatga
    15121 tatttttgaa tctactacta tttgtaacaa taaccattgt ggtcggatca tatccctctt
    15181 tgattaattc ttgcaaatct acttccatca ataaatcgcc tttacgtact ttttttccat
    15241 cagatactgc actttgaaag tatttgccat ttaaattcac tgtatcaata ccgatatgga
    15301 tcagtatctc tgctccttct tcactgacta ccccaattgc atgtttcgtt ggaaaaactg
    15361 ttgaaacttc cccgtttaca gaactgtaga tttttccgtc cgttggaatg atcgcaaacc
    15421 cttctcctaa agatccacta gcaaatgttg gatcgtttac ttcagataaa tcaattattt
    15481 cacctgctgc aggagtactt aacactatat cctttgagga ttcttcttcg atttcatcct
    15541 tgtagccgaa aagaaaagtt agcatacagg aactgatcat cgctaagaaa taactagcaa
    15601 ctagccaaac aaatccttca ccaatataaa tcgggaaaga caaaatactt cttgtagcaa
    15661 ctgccaaagc accggctcct gcggcaccag ctactccacc aaatatagct ccacatatac
    15721 aagcaatgta aaatggtcgt ttatatttaa gagtcacacc ataaacagca ggttcagtga
    15781 ttccaaataa tgctgtgata aatgcagata aagataaact ttttactttc ttatttttcg
    15841 ttttcaaaaa taccccaagt actgctcctg cttgcgcagc tacggcagtc atacatgctg
    15901 gacctagcgt atcacgaccg tagaaagaaa gattgttggt cattactggt acaagtcccc
    15961 aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc gataacagca ccagataaaa
    16021 cagggctaaa gttataaaca gctaaatatc cagctgcaat cagctgactt gcataatctg
    16081 caattggacc tacaactaat aaaataattg ggaccatcac gatgatacaa atgaatggtg
    16141 tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca atataagaaa
    16201 gaacccatac agctaaaatt gctggaataa ccgtacttgt ataccttgct agaatcacgg
    16261 gcatccctag aaatcgcaag gttatgctat cactatatgc actgacaatc gtaggataaa
    16321 cgagagctgc tgcaataacc atagcgatga aacgatctgt attgaacttt tttgcagcag
    16381 tatatgccaa aaatataggc aagaaataga aaaatgcatc agctgctgca ttcaaaatga
    16441 tataagttcc tgattcagta gttaataaat tcaaactcgt acataaggcg agtactcctt
    16501 tgatcaaacc acttcctgcc aagacgccta aagtcggggc aaaaacagcg gcgatcatgt
    16561 ctataaaaga gtttaaaatg ccttttttct cagttcctct ctttccacta gatgcatcat
    16621 cttctagttt catttgtttg gataatacgt catacacttc atttactgca tttcctacta
    16681 cgacttgaaa cattcctcca gcttcgacta cagtgatcac accttctagt tgctctacct
    16741 tctccttatc tgccttctta ttatctttta gggtaaagcg taaccttgtt gcacaatggt
    16801 aaacattgct gacattttct tcgcctccga caaatcggac aatatctttc gctaattgtg
    16861 aatagtccat ttttacttcc cccttgttaa ttaatgccgt agcaatcctt cttacaagat
    16921 aagtatagtt cttagaaaac ggtttctcca ttcaattagt accccttcta atagcgaacg
    16981 aaactgtaca atagtgtgta tggtttactt agccgagcaa atcactcact gcttgaaaga
    17041 aaacaagtat atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc
    17101 tttgctgatg tataagtggt atctcaagct gatactctgt gatttttgtt tctacttggc
    17161 ttttgataag acagcacaac aaagtctcgg catttaacgg aagcataact aggtcagaac
    17221 taggagagat tagttgctga atggaatcta tctttttatg gatttttatt tgattttctt
    17281 ctttgtagta atgatcaagc agttttgttt tctttttttc ttcaactatg gtcagctttt
    17341 cacgcaaaat ctgataaatc tcccaagatt caaactcatc tagaacacta ggtagataaa
    17401 tcaaatcatc ccactcaaaa ataagctctt gctcagtagt caaaattgca tcatattccg
    17461 ttattttgtt tgattgattt ttgaaaagat acactggttc tatccggcat tctttgatcc
    17521 acatgttcat actttgttga atctgttttt ttagtgtatt caaaatacta attggctggt
    17581 tactaacaat cagtatctcc ccttgttttc gaaaaggatt caacgcttcg gcaagataca
    17641 aaacaaaata agcgctttca gctaaattgg gacgtaattg aaaatgtatt gggaagaaac
    17701 gccgaattaa ataaatttct aaaggataac gggagatgat cttttgtaca aaatgattca
    17761 tggtattatg tccggcatcc atcctcgttt tcatttgttt gatatgggtt agcaaaaatt
    17821 ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa
    17881 atgctcttag attcttctct atttctttgt ttttttcatc attaatcatc aaataattta
    17941 actctaatag tcgattttca attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt
    18001 ctgatgataa cttttttgtg agggtctcta ccatcggaat aaataatgta tacctgtttg
    18061 ttttttctaa aagttcttct cttcgaagaa tggtcaaaac gagataccga gaaaatcgta
    18121 tgaagtcctc tcctgaaaca atataattat atgatataag aacctctttt aatgtctttt
    18181 ggacagtcgg catgatattg gaaaaaattg tactgatttg ttgaggaaac cttgcgtgtt
    18241 ggatcaccgt ttctgttcca acaagagata agaaaattct ttttgaattt tcttcagcaa
    18301 tgatttttag tccttttgtc gacgaaactt ccagttctaa tttaggattg cgctcgatcc
    18361 aacgcaaaat cgtttttatt tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg
    18421 ctatcttttg catactgaca tacccttttt gaaacaataa gtacgtcaca atagttgaag
    18481 ctcggaaatc catgctggaa tagattttat gtttcgtcac ctcttgtatg atttgctgtt
    18541 tagtttcaga ttcaattgat tctaaaaaat aacctgaacg tttatttgac tggataatga
    18601 ttttcttctc gtagatagca agcatatttt tgatttcagt ttggatcgtt ttcttgttgg
    18661 tacctaaagc ttcagctaat tcttcgcttg ttatccaatg attcttattt cttaatacta
    18721 caagaatttg gagttgtcgt ttatttaagt ccatttgctt acacctcctc tttgttataa
    18781 acatacagga aaaacctctc aaatgacaat agtcacttgc agaggtttaa aaatcatttg
    18841 aagaaagatc tatattcaag gtttttacca ttagactgga taatttgttt ataatattca
    18901 aaactatctt ttttcaatct cctaaagctt ccgctcccat cattttgtct atccacataa
    18961 ataaagccat atcgcttcct catttcccct gttcctgccg atacgatatc gattggtccc
    19021 caccataagt aaccaatgat ttggcaacca tcttttattg cctcagcaat ttcctgaaga
    19081 tgtattttta gatactcaca acgttttgga tcgtggattc gattcaattc atctggtgct
    19141 tcatctagtc caatgccatt ttcaactatg aataatggta attcataacg gtctgtcaat
    19201 tcattcatga cataacgaaa ccctagagga tcaattggcc aactccaagg ttcgggagat
    19261 ttttccttca aaaagggatt atcgaaacca tttgctccgc cagtatctac gcgcatttct
    19321 gcttcttttt tatagacagc gctacgataa taactaaatg caatataatc tgccgtgtta
    19381 gcttgcaagc actgttcgtc tccatctaac attactggtg cacagtcttc ttccttccat
    19441 gtcctgtaaa tataagctgg ataatgccct cggcacatga catcggagaa aaaccatgaa
    19501 ttgcgtttaa attgcagtga accaaataca ttctctggat cacagtcata tgcataagtc
    19561 gctaatgcag aaaagctaca cataataccc atcttagcat ttggatcgat tttctttaat
    19621 aattgagtag cacgggcgtt cgctagaaac atatgatgac aggcttgata aatcactttt
    19681 tttgttagtg tagcaatggg ttcaagttta tctctagcag ggaatgtaac caatccagca
    19741 gctgcataag gcatacgaaa agcattattg atctcattaa aggtcatcca atatttcact
    19801 ttcccttggt agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc gattaatttt
    19861 cgattttgcc accccccata ctctcctacc aatgctaagg gggtttcata atgactaaga
    19921 gtaattactg gctcgatatc atatcgattt aatgtattga ttagtctgtc ataaaatacg
    19981 agaccagcct catttggctt ctgttcgtct cctcttggaa atattcgtgc ccatgagatg
    20041 cttgttcgaa aagcttttaa acctaactcc ttcagtaaac gaatatcctc ttcaaatcga
    20101 tgataaaaat caatcgcttc atgagaaaga taatggagtg attcgtctaa agcaatctcc
    20161 caaatctttt tttcttcatt ccactgaata ctaggatgct ggttcatgat cccgataagc
    20221 gtatctgtca cattaggtaa tttgccgtct tccaaccatg caccttcaca ttgattagca
    20281 gctactgctc cgccccataa aaaattttct ggaaatattt cctgatccat aactatctct
    20341 cctcgctatc aacaatttct tacttgaagt atagctcgat tcatcaaaac aagatattct
    20401 aactatttcg gcagaagaga agaaaaaaat tgaatgctac tcttcatttt taatatttat
    20461 atataacaaa tacataagac gatccccgaa agaaaacact cgttaaccat ctaagcaaac
    20521 gatttatttt acttccaaca caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg
    20581 taactgtttc agaggataat ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa
    20641 cgtctgcttt tcactggaca tattacaaat aacgagccat tgctggtctt catatatgcg
    20701 agaataggca aagacatgct caggcgtctt ttccactaat tcatagtctc cccagacgat
    20761 gatgtcatgt tttctacgca aatcgatcaa tttcttgtac atataaaaaa cagaatcttg
    20821 atctgctaac gcttcttctg cattgatttc tttataattt ggattgactc gaagccatgg
    20881 aattccagta gtaaacccag catgttcacg ggcgttccat tgcataggcg tcctagcatt
    20941 gtctcttcct ttcgccttga ttgattccaa gatttcctct tttgtaaatc cagaagaaat
    21001 cctttcgtta tacatattga ttgtctcaat atcttttgct tccgcgatat catcgattgg
    21061 gcaattcgtc atgcctaact cttctccttg atagatataa ggggtgcctt tcatcatatg
    21121 gagcaagata gcaaacatct tggcactttc ttttcgatac acatcgtcat ttccccaacg
    21181 agaaacaatt cgcggaagat cgtgattatt ccaaaataaa ctattccatc cttcatcacc
    21241 taaagatgtt tgccatttgg ataatacttt tttcagttca gagatactca agggtttcaa
    21301 atcccatttg tctttgcctt cttgctgatc taatcctatg tgttcgaatt ggaagaccat
    21361 ggataattca tttcttttag gattggaata aagtttagcg atttcggtag ttgctcccca
    21421 tgtttcgcca acagtcatca gatttttgtc accaaaagtt gcttgattca tttcctgaag
    21481 atactcatgc agtttaggtc cattacttgt gatttcttta tccggttctt tgccaatcat
    21541 atcgatcacg tccatacgaa agcccccaat acctttatcg atccagaaat tcatcatttg
    21601 ataaatctct tggcgaacct gtttattttt ccaattcaaa tcaggttgac gcttgctata
    21661 caagtgcaga aaatattgcc ccgttttttc atccaactgc caagcagaac cactgaatat
    21721 tgagcgcaat ctattaggct cttttccatt gaccggatca cgccaaatat aatagtcccg
    21781 ataaggattt tctttatttt tacgggcctc aatgaaccac ggatgttcat cagaagtatg
    21841 attcaccacc agatccatga tgatacggat atttcttttc tttgcttctt caatcagttc
    21901 ttccatttct tccatcgtac cgaattctgc catgattgct tgataattac tgatatcata
    21961 cccattgtca tcattcggag actcgtatac tggagaaagc caaattgcat caatacctaa
    22021 atacgccaga taatccaacc tggaaataat cccccgcaaa tctccaatac catcttgatt
    22081 tgaatcttga aaacttcttg gatagatttg ataaacaact gaatggtgcc accatttttg
    22141 ttccatgatt ttcctcctat aaatggatgt ttctattttt tgcttaatca tttattttag
    22201 tctctctcac taaaaattgt aacgctttca aatagctatt gcaccttgtt atcggtaaca
    22261 gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa ggattgattt
    22321 atgcagggaa gaatgctgat ttcacaactt ctttataaac ggtgttcact cagctgtccc
    22381 ttggtattag ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa
    22441 tactcaggcc agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa
    22501 tcccacaaat acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc
    22561 tgctaacatg gtaattggat agaagataaa acctaatgca atcccatctg aaatactata
    22621 agtcaaaggc atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca
    22681 ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt
    22741 tacttgtgaa gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat
    22801 ccctgtagta atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc
    22861 tacaaatgca ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc
    22921 agaagctaaa gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt
    22981 tgctaatcca actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac
    23041 tacgacccac atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt
    23101 tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac
    23161 cagacctaaa atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac
    23221 aagcaagata gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag
    23281 tgatcctaat cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc
    23341 aaggaaagcg ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat
    23401 cgcatcaatg atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc
    23461 aacgaatacg ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata
    23521 agcaaaaaaa gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac
    23581 ccccattaaa acacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc
    23641 atccattcca gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat
    23701 aaatgttgta aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc
    23761 gaagtaggaa agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata
    23821 aagtccgtta aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa
    23881 aacataagta aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa
    23941 tccctttatc tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt
    24001 taataaagtt tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc
    24061 gtttctgttc ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc
    24121 caaaacctgt atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat
    24181 tctttgctcg aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc
    24241 ttcccattag atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct
    24301 ataacaaaat aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct
    24361 gtgatttctc ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat
    24421 actaaagaag gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg
    24481 aatacgccta attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc
    24541 aattcgattg tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc
    24601 tctacgtcat tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt
    24661 ctatttgatt ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac
    24721 cttgtgtttg atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca
    24781 caaacttttt ttacaaaaaa aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt
    24841 tataattctg cttctgattc atctttggtt agtgtgtgtc taacactagt tacgccaaat
    24901 gtttgatcga ttttttcctt caacgcttcg attgcctgtg cgccacgtgc cgcttgttca
    24961 gcattgatgg attcaatgac cagcaccgct gcttgtgttt cttccgtcag catcgttctt
    25021 tgggcttcac gccagttgag acagcgacag atcgctcctt gttcatcata gtagatgatt
    25081 tcttccggaa gtgccggagc atcggaatct gcacctaaag gcaggaatga ttcgccacct
    25141 tgggcttttc ctaaatgcag atttcctgcg atcttttcga tatcttctcc gccgcaaggt
    25201 acgccaaaag acaaggaaat actattatag atatctacaa gcggattgat tgggaaaaat
    25261 tcccgttctt gatttgcacg tttcagtaaa gcttcaatag aagaacgtgc acctttttta
    25321 gtcttgaatg tggtaaatgc ctgacgccac tgagcaatga cttcgttttg gctgaatgtt
    25381 tcttctgtaa ggtaatttct tgcttctttt gctgcctcat tcaataattg atgatacgtt
    25441 tcttcacttt cttttgttac gtggttatcg ataccattta cggataagat ttcgatggtt
    25501 gcttcaggaa atacttccca gaaattggta tccactatta cttttttcat ctgttcacac
    25561 ctcttgatta gaataattgc ttgatgaata gatagtctgt ttggggatca tctcccatat
    25621 aaaaaacatg ttcgctgtat ttgataaatc cttttttacg ataaaaagcg agcgcaacat
    25681 cgttgtgttc ccaaacaccg agccaaatgg ctgttttttg cttttctttt gctttttcaa
    25741 tagctgtatc tagtaaaaat gtaccgatac cttgatgttt aaattttttt cgtacataga
    25801 ttcgctcgat ttctaaaaaa tgatcagcca tttcctcaga ttgactcgtt cctgtattta
    25861 gtttcaggta acccgcaggc tcttgatcga tactcactag gaaaaaatcg gtttctttat
    25921 cttttagctc ttctgtaagt ttttctagtg cataagcttg tgtcaagtaa tcagctaaat
    25981 gctccggtga attatctttt ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta
    26041 gtaagtctac atctgaaggc gtcatttttc gaatttcgta tttattcacg gcttcttcct
    26101 ttcaatagat ccttttattg ccttttttga caaaattcca atcttcgcta acattcttgc
    26161 atactttatt taaaagatat tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg
    26221 cgactgcatt ggagtagttg ttttctcgaa tgatgaaagg atagatttct gctccttttt
    26281 tagtcggata gagtttcttg atttttttat tttctttatc ttccaaacgt tcgatcatcc
    26341 cattggattc taatttcttg attgctcggg cagtcgttgt acgatctact ttgatcattt
    26401 ctgctagttt ttcttggatg attccaggat tttcgcaaat acgtactaaa taaagatact
    26461 gtcctcttgt taaatcgact tctttgaatt cgatattgct aatagaatct agcgctcggg
    26521 caatcattcc gatgtctcga aggatttcag ccatttcatc ccacctttat tttgtttgta
    26581 agactagttc acctcttttt gttgcaaatg caactatttt caataaaaaa atcaagttct
    26641 tttttaataa ctagtaaagt atattctttt cttcttttac gaaaaagctc aatcacaata
    26701 gatctggttt ttgttttcta aaataagtaa ctgtttcttc gttgttcgct tttctgatag
    26761 ctttggcaac tcttctatac taaaaaatcc catgttggaa gtttcattat tctcaataaa
    26821 cttggcctct tcaccgtgga tcgcacaagc aaaaacaagt ttataatact gaaaaagctg
    26881 tggaatatct ttttgtttat tcgtatcaaa aactgctcgc aactgagtag tttctactgt
    26941 taatcctgtt tcttcataga cttctttacg cacattttct tcaggagaca atcctatttc
    27001 agcaaatcca ccaggaagtg accattcttt cgtccgttta tcttcgatta gtaggacctt
    27061 gccatctttt ttgatatagg cacgaacatc tacttttggt gttggatgtc cttctccaat
    27121 cgttgtgagc gcttttattt cttcgaaagt ttcttttcct atttcactga tcagttccaa
    27181 tgaaatcgtc cggatctcct caaaacgctc tttatcaaat acatcttttc cataatatag
    27241 accagcttga gaaagtgcga gtagtctttt gtattgagcc aacttcttgt tcattctctt
    27301 cccttctttt ttagcaaatg tatgactatg taatcaagta tagaagactg gtttaattac
    27361 ggcaaataaa tataatgaaa tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa
    27421 aagacattcg acttctctgg ggctgtgaat aaatggcgtt caaaagcttg catctgcggt
    27481 aataagcccc accaaacgaa aaatatgacg agctattctt gtctggctgt tcttattaat
    27541 tgaggcagga cagctttttc acaacctccc cactaaacgg tgttctccta ccaactcctc
    27601 ggtcacaagt cacaatattc atcaaacatg agaagctgtt tttttgaaat tgctccttgt
    27661 gactcggagc tgaacgacag gctcacgacc tctcggtaaa cagcgttcaa aagccaattt
    27721 ttcgatatta agccatttct ttgttactta ttgtcattga tttctttttc taatttatcc
    27781 aatagtggag tgtaatccac ttgagtatag tctgttacat caaattcata tagtgttcct
    27841 aactggaatt tgtcatattt tcggtcggcg atgatctttt tgaatgcttc tttcgtaata
    27901 tgattggtcc ctttcgttcg atcttccaat acgtcaccat agtgataatg atccatgata
    27961 tagctcaaat gacgatctga ttcacgatca cgtttgtacc gacgttgcca taacacttca
    28021 aagtctgctg ttaggcgaat cgtgatcacc tgatatccgt atctatctgc taattcttgg
    28081 agtcgtgctt tttgcttatc actaaaagga tactcggaca caatcacttt ctttcctgct
    28141 tccatataca aatcaagtgc tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc
    28201 gcccgttctt ccaaattgtt aaacccgatg gattctgcat agtattcctt gatttcatcg
    28261 agcgtgatgg catacatatc tggaaatcgt tcccgtagtt tattcataag atatgttttt
    28321 cctgtaccag gactaccagc cagaagaatg aaatactttt tcatctcatt tatttcctaa
    28381 gttaattgga ctttccaagt tcataaacag tccaatctta tgcttgacaa tttcttttac
    28441 tgcttcattt gctggaatca cattatggcg aagagacttg ttgatttctg tttcactaaa
    28501 ggtttcttta gctactttcg tccagttgac taaaagctcc gttccaacat tgaatttgcg
    28561 gatcccgtta ttgatcaatt caggataatc ttcttctttc acacctgttc ctccatggat
    28621 aactaggggg acatcaacta ctgcatcgat ttctttcagc aagggaatgt ttacttctgt
    28681 ttttgatttg aattgtccat gattcgtacc gatagcaata gctaatgcat ccacgcctgt
    28741 agcttctaca aatttcactg cgtcttccgg tcttgtatat accttgtcat tttcagcgac
    28801 atggattcct tcttctgtac cgccgattgt tccaagttct ccttctacgg aaacaccgtg
    28861 cgcatgggca tattctacta ctgcttttgt cttcaggata ttttctttga aaggcagatg
    28921 ggacccatca tacatgacag aagtatatcc gctgtcgatt gcttctttga tatcatcaaa
    28981 gtctcgggcg tggtctaagt gcaagactgc atcaaccatt tcttcttctg caatagctgt
    29041 cattacattg actaacacat gcatgccgat atatttagcg gtatcgacac ttgtttgaat
    29101 aatgatcggc gaacccattt cttttgctgc gcctagcata gcaggaagca tttctaggtt
    29161 atgcgtatta aaagctccaa ctgtaaaatt caactcttct gctgtttttg ttacttcttt
    29221 caatgttgta tacatctttt attgccacct tatcttttta tttgtttcct ttgatcgtta
    29281 cttgttttgc aatttgtcgc atctcatcca ttttgcccat gtagtaatcg atcccttgat
    29341 catcaccagc acgagcagca tctgcacgct tctggttata gaggctcatc agttttttat
    29401 acccatctcc gatcgtctgt ttattttcca aactcttttc taaatattgg atggcttgat
    29461 cgttcacata caattcagaa aatttaagtc caacttgttc ataaagtttc gctaatttat
    29521 gcttatcttg ggtagtggaa atctcttgtt tcaattgtgt aatctctgat tgaatctttt
    29581 cggtcgcttc tggcgatagg gctttttctt cttttgccag aacttttttc ggttcttttt
    29641 cttttttctt aaaaaatcca aacatcattt tccctacttt caattaaaat gctttcaaaa
    29701 ctgggcttaa gatccaggca tataagagtg ctgcggctac tgtatctaca tcggttgctg
    29761 tagcattgtt gaatcccatc gaattaaaga tcgttactag caaagcgggc aataatgtaa
    29821 taaagaaacc atgagcaata ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc
    29881 caaatatccc tgctgtccct ccggcaaaga aattggttag cataccaggt aaaatcatcg
    29941 ctaagccgaa catgggcaaa gtaaacatgg caataatcgt accaatcgtt gtcgtaatga
    30001 aacctaaaat gacagcattt gggctataag gaaagaaaac tggacaatct aaggcaggga
    30061 tcgcatcagg aactaatttc atagctatcc ctcgaaatgc cggcacgatt tcacctaaca
    30121 gtagacgtac accagctagc aatacataca cacccactac gaattggatg gcttgtaaaa
    30181 aggcaaacat gatatagttt tgatctcctg ataattcaga agcgaatggt tcgcctgcaa
    30241 ataaaacagt gatgatatat agcgggacca tcacgaccat gacagacaaa taagtatctt
    30301 gcagaaattc gaaatgtgtc ggcaacttga tatcttcgat cgacttttta ttttcgcctt
    30361 tctcaccaaa aagcttagca acaccggctt caaataagta cccgatcgta caaaaatgac
    30421 ctaaagcaat atcatttgat ccagtgattt tacgaatgat cggttgcgct actgccggca
    30481 tagcaatggc gaaacaagct ccgataaaac caccgaccaa gatcaatacg atccctcgca
    30541 gaccggcaaa atagccaaag actgtcgtca tcgttgccat ccataaaatt gcttgaccag
    30601 ttaaaaaaat gtacttccat tttgtgaaac gagccaaaat aatattgaat acaaaaatgg
    30661 ctaagaacgt cagtgcaata tctctaccta aacccaaatc attcattgct tgaccattga
    30721 tagattcaat agaagggatg atcccttgca tatgaaatcc ttcagtaaag attttcccga
    30781 aataagttaa acttccaacg atgatgctcg aaccggcact caacacttga aatcctagga
    30841 gtgtctttaa cgtgccagag atgacttgtc ctgctgattt cttctgcagt agtagaccca
    30901 acatagcaat caatgcaatg gtgatcgatg cctgagtcaa aatattttcg atgataaaat
    30961 tgataacgcc catgttttta ccctcttttc tttttgtatt tagataactc ccttatcttg
    31021 cagaactggg gtgatttttt cttcaatttc attttttgaa acaatatttt tcaaatagat
    31081 catcgccgtt ttaggatcaa ctgcgaactt ttcgaactgc gaacgaaaat tttctgctgt
    31141 gatgatcaca tctggtttca tggaaactgc ggaagaaata tcacaatggt ccagtttggc
    31201 ttctactcct aatttgttca aaacatcctc agcagacatc tgtgctgcaa aactacttcc
    31261 taaacctgca ccgcatacga ataaaatgtt caatttgttc atctttgttc tcccccgtat
    31321 catttattat ttctcaacta ctgaaaaaag cagttgtttg aaatcttcga tatttgtgca
    31381 ttcaatcaat cgatccaact tctcttcgtt gttgatcaat tcaattagtt ctttcatgat
    31441 gttcagatgc gagtaggagt ctactgctgc caaacaaaaa ataatcttta ctggatccat
    31501 ttcaggatta ccgaaatcga ttggttgctc aatcgtagct acgctgactc ctaatttatt
    31561 gaccccatct tctggtcggg catgggctag tgccatgtgt ttgccaatca cgatatacgg
    31621 tccgtaatca ttcactgcac gaatcatcgc ttcgacatag cgctcttcga caatgttttc
    31681 tttaaccaat ggtctagcag ctagttcaat cgattcctcc caagtagtgg ctttttcttt
    31741 gatcatgata ttgctgtccg tcaaaatatc ttttaacata ggctggatct cccttttgtt
    31801 gatttctaaa ttgtttgttt cgaataattt ttctaacttg ccatagatga ttcgatctac
    31861 tcggccacca ctttctttca tgacatctat tatttgagtg aataactccg tcgattcatg
    31921 actgttaggc tctaaccgct gtagttcttc atttcttttc aaaaagtctt ctataaccaa
    31981 gatattcgtc tcagttaaaa tcggatcgac aactaaaagc ggcttatttc gataattcaa
    32041 ttgaaaggtg ctgaaaacca gattgacatc caatttatct actaattcga tttcttttga
    32101 acttaaaact gcagtaatct cgatctgagg aaacttttct ttcagattct ctgctaacag
    32161 atttccagta gccatcccat ggttacagac aacgactgat ttataaacat aattcaagtc
    32221 tcgcttgatt acactggcga tcgtcgaaaa gtggatgaca agaaaagcta tctcgtcctc
    32281 cagtatcttg ccaccaatta cttcttcgat cgtaggaaca aaatgagtga ctgcgcggta
    32341 gattgggaaa taatttgttc ggatgttttc tttcaatgga ttcatgactt ggatatgatt
    32401 cttgattctt acaatcagtg cagccatgtg cttatagaga ttttcgaata aagcttcttc
    32461 ctttagatgg aaaggtacgt gtgtctcgtt ctctacaaat tggatcaatt ggatcgttag
    32521 taattgagct tgtacccact cgattgaatt ggagatatct ctagcattga acgtctcaat
    32581 agtgaaacga agataatttc tttctacttg cgggacattg tccaattgaa attcctcgat
    32641 catgtggtca ataaatgcag aaaagctact gtcttttatt tctgtatttt tccaattaat
    32701 ggcagagata aattcatgtt tcctgattcg ctggatccag atcaatgtaa aaagcaggat
    32761 ttgtttttta taaatatcat cttcttgttt caacaactta ttggaataga tctcctgtat
    32821 ttttttgatt tccaccaaag gaaaataatg gagaaagatt ttttgagccg gtgttaattg
    32881 tgcatcttga ttcataaaga agtcggtcat tcccagattc tgattgataa aatcatagat
    32941 catcgtacgg atagatcgtt ctgaaccttt ataaaccagt ccttgctttc cataactgac
    33001 gatctctatc ccataactga ccagtgttgc tcgtaaccgt ctcatatctt catctaatgt
    33061 acttttagag atttgaaatt cttcttcttt tttgtttaga tggatcggga ttcggctgta
    33121 agcaacggct aaaactagat caaaaatacg ttcgcgccga ttcaaaaagc cgtctttcac
    33181 atcttttatt tcagtttcta atcgagcttg cttttccttt tccagcttca acaaaaaacc
    33241 tttgttgcga attgtacgga tctccggaag ctttttactg ctcaaccaga tattcgcttc
    33301 aagaacttcc tgacggatcg tccgctgact gacattgaac tcacttgcta gatgttttgc
    33361 ttttactgga tattgggctg tcatgattct gtgaagtatt tcaattgttc ttgttttcat
    33421 agaaatgatc tccttctgat ttcaagtata tggttccgct tacataataa aaagtagata
    33481 tttctgccac ttgaagtgga aagaaagtaa gcaaagcaaa caaaaaataa gccatatgct
    33541 ttttcatctc ctcaacgaaa aagacatacg cctattatta caaaaatttt gcccatctat
    33601 acaaaaaaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacaa ctaaactaaa
    33661 atagtgcttc ctatttttcg tttggttgtt cttattatcg cagatgcaag cttttaaaca
    33721 ccatttagtg gagaggttgt gacagagatg ttcagccttg agcgataaga agcgattttg
    33781 aaaaatcgct tcccatattt tacaaaatcg tgccttattg ccgataggct atctctggca
    33841 caccgtttat tcagaaaaag gagctgcgac aaggcttttg tcacaactcc gttcataaat
    33901 atttcctgtc aatccagcat aatcggctga ataaaatcgg cttgccatct tttttctttc
    33961 tcagaaaaat caaaatacca tatctgagaa aaatactgtc gttgtgtctt acttcctgag
    34021 atcaatcgcc catcttttcg atcgactgaa taatcacagc atctgaaatc aattcggatg
    34081 gcaaaactat tttcgtggat ctgtcgataa tttcctactt cctgaatagt tattttttta
    34141 gtatgatttc ttacaccgtc tgctttgttt ttttgcagaa ctttctgatg ttcggcaaac
    34201 agcttgtctg tataacagct tctggctttt tcaggagttt cttcttccca agcggattgg
    34261 atcttcatga atatcttttg gatttccaga aactgtgctt tcttttcttt agccgttcct
    34321 gacatgcggg acaatatctc cgctttcagc cttttctttt gtcttttatt cttgacgata
    34381 gaaaaagcag aaacccctcc tactgacaaa acagctaatg caagaaatcc cgtttgctca
    34441 taactagaag gacctgtacc tggaccataa tacccataag aatctctgcc gtaagaacga
    34501 ctgccaaaat aactgccccc atatgaacga ccaccaaatg aacctctgcc cgaacttcga
    34561 ccgctagatg tacctccacc atgaccaccc gcgacagcga atccttgggt cggcagagca
    34621 aacaaacata ggcccactaa caaaatgatt gctaaataat tgattttttt cattgtcttc
    34681 ctcgttatct tttattacaa aaaactattt ttatgatagc cttgctaaaa ggaagagtca
    34741 ataaccagat gcataaactg tttttttagc aattttgtct tatttagtca ttgagtaaca
    34801 tcggacaaat gtatgtgtcg gcagtccata attcctctat cttcagcatg aacaagcttg
    34861 cagacaagag atctgcgctt cctccaggag agatttggtt ttcctgacaa aagtgcaaca
    34921 gtgctgtaaa tggacgattt ggtaaagcac tttgactagc agcatgagca agtttttgca
    34981 tttccactaa cgttttctga tcactgcgat gcaatacgca tgtgtcatct agcgaactca
    35041 tcaaaactaa caacatatgc atttgtttct gtacatatga atcattatct agctccttag
    35101 ctgcttgaat tgcaagcctg atatgcggat accctaatat cgcttcttca tatgcaccat
    35161 ttacgccata tttttgcttc accctatgac catgagtggt ttttttatga cgatattttg
    35221 tatcaggaaa agcagcaagc ttgctgacat catcaagtat tttcgataaa gaaaaaggct
    35281 gttccatact gattttttgt gcaatgacac tgatcaatag ccccattgcc caaatcgctc
    35341 ctttatgggt attgatgcca tttgtagcaa tcagcattgc cttttccgct tttcgcccaa
    35401 tctcagcaat tctttctctt aactcctgat caatagcatg atgccatgct atttgagcca
    35461 tttcttcaaa aaaaggagtt agcgtcttag cagacttaag aaataaagca tagtccatat
    35521 cggaatgtgc acccgcatcc agtgcatcca ctaatccagg ttttggactt aatgttactt
    35581 ctgcgatcaa tgcttcctct accaacgcag cgagttgctg agtcactgct ttttttcgtt
    35641 cgtcatttat catgacactc actccttctt cctactatta cttgctgaaa ccttttttga
    35701 tagctaccca aatgatatga agtaaccagc ttaaagcaaa tgcacaactt gccgtcatta
    35761 gataagctac aagaatcatg actcctgaag gtaactgcag ccaactatct ttaaacaata
    35821 ataacagtac ttggaaaaca aatacattgg ctaaatacgt acgataagca taaacagaca
    35881 accatttgaa caatggcaat tttggcgaat cgtttacaat cattttggaa gcgcctatga
    35941 agacagcaaa gatgatgact aaactgtata aactttggat cgtattcaag taaggtgcat
    36001 tcgcaaagct caagtctcct ggatgtgcga gcaatgaacg gacagaaaag aacgcgataa
    36061 ctaaggcaac aggtaagaaa agataacgta ctctgtccaa atattgatag actttttcgt
    36121 gataaacaaa tgcggctacc ccataaatca tgaaactagc aacaaaccga tctaataagt
    36181 accagctgtc atgataaggc ccagtcaata cttgtgtttg atagaacaga taccatgcga
    36241 catataaaat cgttgaacca atcaatactg gccatacata ttttttatta gcaaatactt
    36301 tgtaaccgag ccaaacaaaa aacggcatga acaattggat ctgcagcatc ataacggtat
    36361 accataaatg aggcgctcca tcacctgtga catatttcag taaaaagctt gcagtatccg
    36421 tatacggagt ttgttgttgg actgctggaa atagccaaag ataagccgtc gtccatagaa
    36481 gatacggaac gactagttca aaaaactttt ctttcaaaaa ttcccaataa gacaaatgct
    36541 gacttgtttt taccatatta taaacaattg caaaaataaa cattggtgct gtgtatttaa
    36601 cgatgacgta aaaggtacca agaaaagctg tatttgttgc gctgtggctg tttgccaaag
    36661 caaagctcaa tatcgtttgc atcataacag ccgttacagc tgatactttc tggaagtcac
    36721 ttacgttgat tttgattttt ttgttttcaa acatctcttt ctctctcttt ttctttatta
    36781 ccaaagttct ttggttaatt taggtccttc gactgttttc aatagaattt ttttctctgg
    36841 ctctctcaaa tactctttta gtgaaaaacc gccttgccga gtcactactt gggtatcgaa
    36901 ttgctcaaaa aaagcaggat gcgattgaat tacctgaatt ggcaattcaa tcggtgaatt
    36961 tgcgtacagc agcaaatcaa aatcactggt ttcttttact gccggaatcc cagtcgataa
    37021 ttcaaatcct aagctgccgc cgattcccca tttacatcct gataatatgt ttctagcagc
    37081 atgaaattgc tgataaacag gataatccat gattttctct tgtctatacg attcttgttc
    37141 gatgatgtcc cacggatgaa tgacttgttt gacggcatgt tttgagattt ctgttgacca
    37201 tctctgattg cgggcaggtc cacgtaaacc aatcggtagt atttctgcgt gatccgtcga
    37261 cgcacgacga actgttccga acatcttttc agttagaacg acccattcag gcaaggtgaa
    37321 cccttctatt tcatgtttat caaaaaggac aatatcatgt gcttgaatca ttttttatgc
    37381 atcccattct tcgtccattt tggcacggac tttatttgtt tcgacacgtc cgaatgtttt
    37441 ggctgtttcg tttgtgtatc gatttgtcag catcgtgtca tttgtattgc ggacatcttg
    37501 aatggcttca ttgattgctt cgatgacttt gctgacattt tgttctgaag cagcttggtc
    37561 ggtgatatct tctaggaaat gatagatcgc tcctagtttt gtataattat tgatatcgta
    37621 agcgatagct ggaacttttt cagcagctgc ttcgatttca gcaaccgaac gttttgtcac
    37681 acgtgcagca ctttctttag acatcgcttg gacggtaatt tctttattgt ttaaagcaat
    37741 caagcggctt gattgtaatc catgtgctaa aaatgcacct gaaatagcat ttcctgcaat
    37801 caatccaata actggatgtc cagcttgacg gagttttgca taagcagcag cactattagc
    37861 caaagccaca tggataccga tcaattcttc gttatagccg tatgcttggc ttggtacatc
    37921 gatcacaacg atgattggcc gtttcacttt tttgtcttta tcttcttcgt aaacgtgatc
    37981 caaaatttgt cccatgcgga aaccttcaac taaaccaact tcaccgtttc gtacacggta
    38041 catcgggttt ttaggatcag gtacgattga tgtcacgatg gcttcttcta tgaatttact
    38101 tgatttacca taatacacag tactaataga agaagttgga tttttcatgc cagttagtgc
    38161 cgcaaaccaa tcgtaaccaa cacttgctgc agctggttca tttccttctg ctacttctgg
    38221 taagtcaatt gattttgctt gatggttagc aattgcttca ttgtaatcgt caatggccat
    38281 tggttttgaa agatctaaag aatcaagtaa tgataaataa aatgctgagc tttcactgcg
    38341 atgcgtgtct tttcgttcgt ccattgctgc gatgacagca gctttgattg cttctacact
    38401 atcagtcact aattcatcaa tgatttttga ttctacccgt tgacgagatc ctaatgtatt
    38461 ccaaatcaag tctttgtcac tagaatcaaa ttctcgaacg cctgcttctt gttcaataac
    38521 ttctggtcca ttcaaaccga ctctcgcttt ttttgttgcg attaaataac tcattaatgc
    38581 tgaagtaatc gacataccgc caaatgatcc tacacgtcct ggaatcaaac cgatgactgg
    38641 tacatattgt tttagagcaa tgattacatt tccgatttct gaaatagata acagtccgta
    38701 gtttgcttct tgcaaacgaa caccacctgt atctagtaca atgactggaa agatctcatt
    38761 tcctttttta ttttcttcta acacatgttc aagtgcagca acgatttttg caccggaaac
    38821 ttcaccgata ccgccacctt ggaattttcc ttccatagcg attactactg cttgtttgcc
    38881 attgatcgtg ccacggctga caacgatccc gtcatcactt tcagggacga ttccttgtgc
    38941 tggtaaactt ggcgcgatga tttgatcgaa tggatcaatc aattcacgtc cattgtcaag
    39001 tagagcgttc actcgttctc tggcgtttaa ttctacaaaa ctatttttca tctgaaagtt
    39061 cctccattgc ttgcgtcaat cgtaggtaca caactcctgg tgtagcaccg aagtcattga
    39121 tcacgatatc tgctgaaatt ggataacgat caaaaaatct cgttaataca ttgttccata
    39181 cttcgccgaa gccatcgctt cctgtacaaa tttttacagt agattctgtt ttttctgttg
    39241 gaaacatcaa gatttccaaa tctcctgaac cgaccactcc tacatggatt ttcttattga
    39301 tcggctgttt cgttggatat ttataagcta attgttccat ggatctctcc cctttttgta
    39361 aaacagagca tacacgttct gcttattcgt ctttcttctt ccatctgggt aaatggcgac
    39421 aaaaggtttt tctcgccaat tttatgacca agttctgaat ttcattggtg gttcatacag
    39481 gccatctgac cattcgatca aatcatcgat ggtttgtgca gctaataatg aacgtttcgc
    39541 ttcactacgt ttgatgccta gatcttctgg taatttcacg atgccggctg cacgcaagct
    39601 atctagtgat tctttcgtgc tagtcattcc aagaggtgta acaccgccaa ttgctgcaat
    39661 tgctgcttgt cgttcttctt ttgtttttgc tttgtacaaa taagcaattc cttcttctgt
    39721 cacgatatgt gtagtatctt cgctataaat catcactggt gcattggcta agcctgcttg
    39781 tttttgaaca gctagtgcat ctaattcttc gacaaagact ggttttttat ttgaaccata
    39841 tgtttcaacc atttgaacaa ctaatttttg gcctttccct aatggatcat cattgtcgcg
    39901 aagtgattgc catgctggtg tagaatgacg acgaccgcca ggattatgtc ccatgttagg
    39961 cgcaccgcca aaaccagata aacgtccttt tgttacggta gaagagtttc cttcgtaatc
    40021 taattgcaag gttgaaccga taaacatatc gatggcatat tgtcctgcag cttgtgataa
    40081 tgttcggtta ctgcgcatcg tgccgtcttt accgacaaaa aaggcatctg ggcgagcagc
    40141 aatatatttt tccataccaa cttcgccacc aaaactatgg atgctttcaa cccaacctga
    40201 ttcgatggct ggaatcatcg ttggatgtgg gttcaatacc cagttcttcg cgattttccc
    40261 ttttaaccct agtgattcac cataagttgg cagtaacaat tcgatagctg ctgtattgaa
    40321 tccaataccg tgattcaaag attgtacgcc gtgttctgcg tagattcctt tgattgccat
    40381 cattgccatt agaatttgca attctgtgat gttttgagga tctcgtgtga ataaagcttc
    40441 taattgataa ggtttatctg ctttcacgac aacgtctacc cagtcaccag ggatatctac
    40501 acgtggtaaa gaatcgacaa cttcgttaac ttgaacgatc acgataccat ctttgaaagc
    40561 tgcagcttcg atgattgttg gtgtttcttc cgtattgaaa cctgtgtaca agttgccgtc
    40621 tttatctgct ttatctgcag ctactaaaac aacatttgga atcaaatcga tgaataagcg
    40681 tccataaagt tccaaataag tatggatatc acctaatttc attgtgccgt cttcaatcat
    40741 ttgggcgaca cgcaagcttt gtgcaccagc ataagagaaa tcgatcttgc ttgcgattcc
    40801 tttttcaaaa atattcaaat gttctggtcg tgagatacta gacatgatca aatgcagatc
    40861 attcactttt tctgcatcta ctttttcaag actttctgaa agaaaacttg cttgtttttg
    40921 attgtcgcct tctaatacaa ctttgtcgcc gggagcaatc agtttttcca ttacttctac
    40981 gattttttca gtttctacat acttgccatt gagcaagttg ctgatttttt ccaatcgctg
    41041 cgcttttttt tcgcgtttag aagcccagtt gcggtttggt ttttccataa ttagtcatct
    41101 ccatttttga tatagaaacg tggcgtcatc aataccatgt tggtctagat tgatcgtacg
    41161 gatcccttta tctacaccaa attttgtatg aatcagcttt gtcaaagtat tgccgggggg
    41221 aaattcaatc gtcagatcca ttcctaattc ttttgccaag tccatcattt ggttccactg
    41281 cacagggaaa gctaggtttt ccaacaaatc tttacgaatc tcttctgcgt cctgagtggc
    41341 tcttccatcc gtatttttta tatacaaaca tctagcatca gatagatgga cagactgcat
    41401 atacggcttg aatttcttga ccgtatcatt catcaagaga cagtgagatg gcacagggac
    41461 tttcagcagc ttcgctgttt gcgcttggtg ccgtttggct tctgccaacg taacggataa
    41521 accttcgata ctcccagata acgtatgttg catcggacaa ttttcatttg acagataaac
    41581 tggatggtca ggatcaaatg tttttgctac aatttcctca gcttcgcttc tcgtcacacc
    41641 tactactacg cccattgcat aaccgcttgg ataagcttct ttcattaaga ctgcgcggtg
    41701 atagactagt tccgctgcat tctcaaatct caaagatcga caagcaacag ctgcacaaaa
    41761 agcaccaatc gaatgtcctg ctgcaatatc aggctgaata ccgttttttt ccatttcatc
    41821 taaataaaat actgcttttg tcagtaatgc tagctggata aaaaccgtat cttgatagtt
    41881 ttcttcttta tcaactaacc gataccccgt cacattttct aaacgttcca tgtacatttc
    41941 agggatatcc gctaacatcc ctaacttctg actgccttgt ccaggaaata agaaacctgt
    42001 actcatgagc ctcgcctcct cagcacttac tttttccact gctatcttag tcaatttctg
    42061 ctataataaa aaatgccaaa aaaagaaaaa accataacaa aaaggtatgg atgggatgga
    42121 taattatgaa attgcttcat ctgcagtatt ttaaaaaagt attcgaaaca aaaaatgtta
    42181 cccaagcagc caaagaatta tttatttctc aacctgcctt gagccgtgcg atcaaacatt
    42241 tggaaaacga attaggtgtc cctttgtttt accatagcgg cagaaacatt gagacgaccg
    42301 tttatgcaga agaattttat ccatatgcag tcaagacatt agacacatta gaagaaggaa
    42361 tcaaattatt gtcttcggtc aatgagaaga tcgttacttc cgtcatattg tatttagagg
    42421 tagcctctgt ttctattccg aacttggtcc gaatttttat ggaaaagcat cctgatatcc
    42481 aattaacgat catgcaacat catttaccag atgatattac cgatcctgta ctgtacatca
    42541 cttcggagat gaagcctggc atgaccaata ttcctatcat gaaagaacct gtttatgtcg
    42601 ccattcctaa gaaacatcct cttgctaaaa aagaacatct gcaattagaa gatatccaac
    42661 gcacccccat cttgatgctg tcaaagagca acgccttccg tcatacactg gatgcagcac
    42721 ttgaagcgaa aagtatcgaa ttgacgatca gttcgacgac agatgatcct gcgacattac
    42781 gttctattgt caagcaagga ttagctgtta gctttttccc aaaagtttct tggtcttacg
    42841 ataaaaatga tcctttcgtc ttgcgggaaa tcaaagattt gcccttgaca cgaacaattt
    42901 atttatcttc tagcttcaca gaagatcatc ctttgatcaa aatgattgca aaaacgatgc
    42961 gggaatttta tttaggaaaa taaaaaaaaa agagctggga caaaattctt gtcgcagctc
    43021 ttttttcgaa caaacggtgt tccagaagta atttcttaga aaataagccg aaatttccca
    43081 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctaa acatttctgt
    43141 cacaacctct tctctattgt cctagacatt cattgcgttg atttaaaact tccatcaatc
    43201 aaatctgcta gttcatctag cttttctatt ttacgtagat caatagagat ccaatgttct
    43261 ttattcatgt gataggctgg aaaatacccc tcttttcctc gcaaaattcc gccaagttca
    43321 ggatcgacct tcaaatcaag aatattgatg atcctatggt cacttgcatc atcatagatt
    43381 ttttcttctg gaacagacat caggacccca aaccattttt tgcttttttg atgtctgaat
    43441 acgcaataat caggaaattt tttaaaaata tattctggag acaaattgta tgtttcattg
    43501 atgtgatcga ttagttcttt tttcgttagc atctgcttcc atctctttct tattgattcg
    43561 ctttattcaa agataccacg taagaataga aaaatgaata caagtagttg ctaatcaaat
    43621 acttcgatat ttttcttttt tatcaactct taaatgttca atccgcctgt gttggtcttg
    43681 ctaccatcta ctattaaaat gtttattatt gtttgcccac ctattttttg attttagtgg
    43741 tatcattttt caagttcatg atgaaattct tatcatcagt aaatgctcca gaaaaataaa
    43801 caatgattga aaaatcagtt gaaacttgac caccgtttat tttctccgta ttcttagaat
    43861 agaagggagt ctgattgatg tattcactaa tgaaaaaaat cattaccgaa aaagatattc
    43921 gacgtcatgt ttctttagta gaacaattgc tgaatcatac aaagataacc gtaaatgaat
    43981 tagctgaaat catagggaca accgaacgaa cgattttttc tgaccttcaa tctattcgct
    44041 ctcagctgcc cgaaggctgg gacatcatca gcgatcaagc aggaatcagt ttgcaaaacc
    44101 agcaaaattt attaacaaat gacctttggg agattttttt caaacaatca gtcagtgtcg
    44161 aactgctgaa aaatctgctt tttacaaaaa aagttgctgt ccctgatttt ctagccgacc
    44221 atgggctttc ttatgggaca ttgaaaagac atgtgactaa aatcaatcag cgtttagcaa
    44281 gttacgactt gcagatcgat ttgacgaaat atacagcttg tattcttgga aaagagcgtg
    44341 cgattcgtac tttttatcat cgattgctga ttcctttcac tcataacaac tattttttcg
    44401 aggattattc gatccatgaa tcccattatt ttcaattttt aagaaacctc agtcaaactg
    44461 aactagcggt tgaaacagaa gagattttcg gaacatgttg gttctttatc aatacgatcc
    44521 gtataaaagc gaattgccga ttagattctt ctatccatac caacagcaca ctctcctccc
    44581 tgtatgactc tgcattgaaa aaattgtatt taacggaagg tatttatctt aaagacactg
    44641 agctttcttt tgcttctttc tgctttctgg aaagttggaa ttacaacaat aactatggac
    44701 aagaaaccgc tcgctgcctg catcattctc catttttaga ggtcctcgaa acgtttgtag
    44761 aggaactagc ttcagagctg tctcttgatc aactaaaaaa aacatctctg actgataact
    44821 tggctctcct catattaaaa tatcatgaat cgccgatatt gattgaagca ttggaccgtc
    44881 aataccatcg ttttttagaa acatatgatc agcaatatgc acacttgtat caacagaaag
    44941 agaatttact gaacgatcta aaaaaagaaa tagaaatcaa tgaaccaaat tactttctcc
    45001 aactcttgtc tctactgatc caacaaacga ttttttctat caaaccgaat ttgttcaatg
    45061 tctattttat tttccaagga gaaccttcat ggaaggcgtt cttgcagcag gaattaaaag
    45121 attacttagg aaaacgtgtc tgcttcttac ctatcgaact aaccgatctt tccactattt
    45181 cttttgaaaa aacagatatc ttgatttcca atttccctct agattctcta gatatcccta
    45241 ttgtatacat ttcttctatt ccaacgaaaa atgagctcaa tcgtttgact gaactcactt
    45301 tcaaaacttt tttatagtaa cggctagcga ctgtattctc tagctgtttt ttcttttttg
    45361 cgaaacagtt tgttcatgat ccctttttct atttctacaa ctaagaatag aagaatcaca
    45421 tttgctccaa tcaataactg ttgtgtcatc gttaaactta ctgtaccgat cagctgctgg
    45481 ccgactggta gaaaaataac catcccttgc aataaagcaa ggatacctaa tgaaagaaaa
    45541 agcgctttgt tttgcaaaag accttggttc aatgacggct tggacaactc tctgcaattg
    45601 atcatgtaaa cagcttgagc taagacgata ttttgcagaa gaatggtttg ctgtaagctt
    45661 tgcccttcaa actgcatagc caataaatag gcaggaacca tgatcagtag ggagacatag
    45721 atgatccgaa acaaatgata accagatagg attccttgct ttggatctct tggtgggcgc
    45781 ttcatgattt ctggatctgg cgactcgaac cctagtgcat aagataaagt gattgtcgtt
    45841 accatattga cccataaaat ctgtactggg gacaaaggta gcgggcgatt cagcataagt
    45901 gcccaaatca agatcaatcc ttgagctaat gcagttggca agaagaaatt gatggttttt
    45961 tgcaaattat ccaaaatccg ccggccctct tttacggctt ttgcaatcgt atggaaatta
    46021 tcatctccta aaaccatatc agctgcctgt ttgcttactt cactaccttt gattcccata
    46081 gcaattccta catcggatct tttcaaagca ggtgcatcat ttacgccgtc tccggtcatt
    46141 ccgacgattt ctccattttt ctgcaaagct ttaacaattc gcagtttatg ttctggtgtc
    46201 gttcgagcaa agacatcgac tattggcact tgctgaatca actcttcatc agacataaga
    46261 tccaaatcga tcccttctaa aactttggca gtatgtttta agccaatttg ttccgcaatc
    46321 gcttttgctg tgtctttgtg atcccctgta atcattttca ctgagatccc tgcttgttgg
    46381 cattccttta cagctttgac tgcactttct tttggcggat cgatcaatcc ggcgatccct
    46441 gcaaaagtta attgggttaa agtctcatgc gtgagttcct gtttcgaatc tactttttta
    46501 taagcaaatc ctaaaacacg ctgacctttt ctcgcaaatt ctgcagctgt ctgactccat
    46561 gcttcttgtt ctgcagaaga aagagttgat ttagcaaata atacttctgg tgcacctttg
    46621 acgtaaatca ccgctccttt ttcctccgca tgcatcgttg ccatatattt gtattcagaa
    46681 ctgaacggaa tctttgcttc tatttttttc aaaggtagat tggctgtttc tgtgtgtcga
    46741 atcagcgcaa cttctgtcgg attgccactt aaattttctg ttttctgttc ttcttttgtt
    46801 tttaattcct gacagttttt cataatggac aacacgcagg cttcttcttt tgcagctaca
    46861 tccacaactg tcatttcatt ttttgtcaat gttcctgtct tatctgaaca gatcactgtc
    46921 atagaaccta atgtttctac cgaaggaagt cctttgatga tcgccttttc ttttgccatt
    46981 tcatgtactc caacggatag aatcatggtc aaaacagctg gcaatccttc gggtaccatt
    47041 gacaccacca aagcaatcgt agccgaaaga aggatatgcc aatccatacc atatcgaaat
    47101 gtcgtaaaaa taactaagaa taggctcaaa caaagaatgc cttgaaaaat ttgtttgttt
    47161 aatcggttga tttttttaat cagaggcgtc gtttgttgtt cgaccgattg caatgcttga
    47221 ttgattttac cgatttctgt cgtgtttcct gtttctacaa cgacaccgac tgcagaacca
    47281 gattgtacaa gcgttcctga aaaacccata tttttctggt cgccgatcga ttcttcttct
    47341 gtcaaaacgg catgactttt ttcagatgct tctgattctc ccgtcaaaat cgattcttcc
    47401 actaataaat catgtgcttc gattaatcgg atatctgctg gaacgacatc tccggcacgt
    47461 aagacaatga catctccttt taccagttct ttcgcaggga ttttttcttg catcccgttt
    47521 gatagtacta tcgcttcttg tcccatcagc tgcttcaaac cattcaatga ttcttccgct
    47581 tttctttctt gccagtaact aacgagtcca ttgacgatca ctacgaataa aataatactt
    47641 ccttcaatat attcactaga agcaaatttc aaaaaagagg caatgatcaa cacgatcatc
    47701 agcagatcag taaaatgctt tgctattttt ttccatcgtt ttgtctgctg ccttgctttt
    47761 atttcattat atccttgatc agtcaggcgt ttctgtcgtt cttcttgcga caagccttct
    47821 gttgatgttt gatagttttt caatacatct tctaccgtca atctgtacca gttcatccta
    47881 tcccctcctg tttaattgat actgttatta tacgcaggga agttttttca ggcgtatcga
    47941 ttatttcagg ggggtctgaa ataattcgtt ttgcaaaatg tatctgggcc taactgatat
    48001 ttctattgct tattctttta tatacaaaat ttaagaatac aaaccagata accaataaaa
    48061 aaggaataaa gcgtgtcgtg ctttcgaata acaagctgaa agcaattgaa gtaaaaaaca
    48121 ccaacaaagc aagatcttta tatctttgtg tagtgttccg tttcttttct ttaataaatt
    48181 gaacatgtgc acacaagatc gaagaccaca caaatagaaa acaactcgtt gtcatgcctg
    48241 ccaaaagctg aaaaaacaat cgactatcag ctacaaaaaa atgtagacaa agaccggcaa
    48301 aaaataacat tcctgtaatt aaaataccgt gtaaaggtac gcctttctta gaaagcgcac
    48361 aaaaccaatg gggcaaactt ttttcttttg ctgtttgata taaaatcctg ctagtactgt
    48421 agactgcact atttgctgag gacaaagaag caaccaaaat cattagattg acaattgctg
    48481 ctccgccttg gcaatggatc acttgcagca tagtcactaa aggactttct tttaaatcta
    48541 aacaatgcca tggaacaaca aataaaatcg acaaaatcgt tcctatatag aaaaagagaa
    48601 tacgccaagg cagacagttg attgcttttt tcaaattctt ttgcggatca cttgtctcgc
    48661 caacggttaa gccgatcatt tcaattccag caaaactgaa taaaaccata ggaaatgaga
    48721 ctaccattcc agcaaagcca aacggtagta attcatcgat ctgtccaaaa ttttctttga
    48781 ttccgataga ctgggtatga aaatctgaga cactcaatcc tatacccaat aagataaaaa
    48841 ggaaaatccc caagatcttg atgattgaga aaaaagattc caattcacca aaccacttga
    48901 ctgcaacaag gttgactgcc attaagatca ttagtgcgct acctccgctt atcaaagctg
    48961 gtacatgtac aaaccagaaa tggatataca atcctattgc tgttaattcc gcaagtccag
    49021 ctccaagcca gcaaagccaa taagtccaat tgacaaaaaa tgcccacttt ttgcctaaat
    49081 aatagtcaac gaaatcactg atagagttaa atgtactatt tgctaaaaat aaatcaccca
    49141 tggcccgcat caaaccataa atagccaaac cgcatatcaa ataaacgaga ataacagctg
    49201 gccctgctga tgcaattgcc tttgctgaac ctaaaaataa tcctgttcca attgcgccac
    49261 ctaatgcgat taactggata tttcgattag ttagagtccg tttcattgcc ggtttttcca
    49321 tcaagttcac ctttttcttt ttcaagcagc atggcgactt cctgttccca agaaggtttc
    49381 tttatttttt caatacagag tggtactaca aacaaaatga cactgatggc tgtgactgca
    49441 attagattga tccatgttcc agctttcatc gtcagtccga cagaaaatcc gattgccgca
    49501 aataaaacaa taacgacagt ccaaactcca agattgctct ttttctcttt ttttccaatt
    49561 ctaaatggtc gattcaactt tgcatgtgta taacgcaatc tcaaaactgc gatccccatg
    49621 atgacataga cgatacagta gataacagta gttgaattta cgagcatcaa aaatgcttga
    49681 ttgacgccag gaatcagcaa ataaaaaagt gcaaagattg agataatgac agactgagtc
    49741 aaaataacgg atttggatac gtcaaattga ttcgtcttcc aaaaattgaa ttttggtgga
    49801 tacgcacctt ttctagcact ttctgtcact gtctttgctg gtcctgaggc ccaagcagat
    49861 aattggactg ctacaccaat gaagacaagt aaactaaaca gattcacaat aagatgtggc
    49921 aaaccaagaa tttggcaata aatcgaaata gattgcgcga tattattcaa ttccatttgt
    49981 ccttttggta cgacatttgc tacaactaaa gcattcaaaa tattgacgaa aaatataaaa
    50041 attaacgcgg caaatactcc gcgaatatac gttttaactg gattttccaa tcgagtgata
    50101 tacacagagg acatttcgat accagtaaaa ataaacatga tcggcgcaaa ataaacaaat
    50161 gaggatgcag tcgtagtatc tggtattaat ttgtcccatg aaaaactgcc taatgttccg
    50221 gttggtacga tcccaacttt gatccacgca gcaaacccta ataagagcat catcgtcaaa
    50281 ggcacgtaca atcctaacca aacaccgact tttccgccga tttttgccat atcaaatttc
    50341 aaattcaaaa aagtgatgat ccaataaacg actaaaatga caactaatgt aaatttgctg
    50401 ttcaaaccaa gcggcacatt atcgataata ttaccaaaca acggagcgag tgctgaagcg
    50461 accatcacca tcccaggaaa catttggacc cataacagcc atgagacaac gaacccccat
    50521 ttttttccta gagcattcga tacccaaagt tctggaccac ctttttgggg aaacattcca
    50581 gcaaattcac cagagatcaa tgagattgga aaagcgtaca ataatactgc aaccagcatg
    50641 taaaaaatca tcgtccagct cgttgctgct acatctggaa tatttcgtac acttgctaca
    50701 agggcgcagg tcattccgat aaaggtcatg gtcgtgattt gctttgatga accagtatct
    50761 ttcatcttta ttccttcttt caatagcgac atatgttttt gttttctttt atttttgttc
    50821 aagaacagaa atgacaaggt ttcatcgatt cagtacaaaa cccagtatca ccgaaacatc
    50881 cattgaacga tcttagtgaa ctgggtttga tacatgaaac gaacgatcca acttatagaa
    50941 cgttttataa aaaagaatat cttggcgagg aatatatgaa aaacaggggg aaatacaatt
    51001 gttttgatga agcaaataat tgtctgttct ccatatgtct cttgttattc tttcacacaa
    51061 aaatcagttc tattggatga tttcgtttag tttcttttcc attgctttgg cgattttttt
    51121 tgtataaaaa ttgaaaattc gatcatcatt caaataagga gtcatgatag ccgcacgtag
    51181 cagagtgatt ttctgttctt ttttccattc ttctttcgtt agacccattc tttcaacaaa
    51241 acgaatcgga gaatcaccgt aatcttcttg tgtaaatgta gtatgagaag taataaaccg
    51301 ttcaccataa acatcgccat ccatataaga agaccgatcg aacatttttt catttaattc
    51361 attgatagcg ttcaaatctg tacagccttg ttctttgaat acccaattga ccatattaaa
    51421 atcaggatga ttcaatggat agacttcgat tgtttttcct ttaacagtaa aagtcagatg
    51481 atcgagaaat tctctgaatc tttgagctgc ctcgattgaa gccccaatca attgaccata
    51541 tccggtcaca ttcaatggca atacacgatg tgctgtccag actgcggcgg cggttgctcc
    51601 agcttttgat ccttccaaaa tataagctcc taacatatct ggagccttta ctgatttttc
    51661 aaacacatat ggcgcgaaat aagaaatgat atttctcata tttttatgtt tgattacgat
    51721 tcctccagca gcataaggga catagcccat cttatgagga tctatcgtga cagaatccgc
    51781 ttctgaaata gctctgaacc cttcatatac ctctttgtca atcgttacgc agtggtggaa
    51841 aacatgatgt tcttcaaaga attcagctaa ggaagcatac ggcacaaatt ctcctgcttc
    51901 gtttagaaac agtgaacgcg catagccgcc atatgcggca tcaacatgta aatagaaata
    51961 gatcccttca tcttttaacc tttctcttaa ttggacgatt ttatctacac tgtctacttg
    52021 accttcttct gtcgttccta cgacagcaac cacacctaga attgggattt tttgtccagc
    52081 taattcacga attgtttttt ccaaagcatt aatatccatt cgataatcct cttgaactgg
    52141 gatagcaacc atctgatcta agccgactcc gcaaatatct aacgccttca tccaagaata
    52201 gtgttttgtt tgagggacca gccatttccc taatctttgg atattttttc cgctacggga
    52261 agaagctgct ttgacctcat ccatttcttc gtccgtaaag ctttcggtca tttccaaaat
    52321 ttcttcaaca gataaattca aaagttccca ctcagacatt tttttcactt tttcaggcag
    52381 cacttccttg acagctaaag gaatcgattt gatgcaacgg gcgtaccata atccttcaag
    52441 attagcaata gaaccatctg ctgcaatgtg tccccatccg tctgtcatat tgaataatga
    52501 agcaaaatcc tgtcctactt ctgcttccat ttgcgaagtt gccatagaag attccaatgc
    52561 aacattattt gggttccaca gcatcgcata gttgtaagct aataaagcag gcatcaatgt
    52621 ttcagcattc atttgtcccc agtaacggcc tgctgaatgc catgggatcg atcctgcgcg
    52681 taatcgctga ctgacttcat ccaagacctc tatcatatgg tcgcgggtgg ctagataatc
    52741 tggagtgagc ttatctcctt cgctaattgc aggcatatct gaaggaatat aattttctct
    52801 ccatccaaga tgttcatcta ccatcttgtt caacaacatt ttataaactg gtccattttc
    52861 agctttgtcg cctataaaga cggccttgat atccatatct ttcatttttc ttccctccat
    52921 attttgttgg tcaatgtctt gcagatacat gtgtttatct gtaacacctc ttcactatag
    52981 cgccaaaaat gagaattttt tttttataac atcaaatcca cgtagatttt tacaaacaaa
    53041 aataaataaa gtcggatttt tctgataatt caacgcaaaa aagcgaacaa tttttcaata
    53101 aatcagtctt ctaaaataga taacctaaaa aaagcgaaca aacaaataaa atgactgtcc
    53161 ttcattcact aaacatattt ttattttttt atattgttaa aaattcgcaa tttatttttt
    53221 aatagaatta tctgttttcc aaattttata acataaaaaa ccttttttaa cggcatttat
    53281 atgctttttc agaaattaac tgatgatttc aaaaagaaaa catgtcataa tatttcccat
    53341 attgtgcaaa aaaatacaag gcggaggctc tactatgatt gaagaatata ttgagaaaaa
    53401 tattttaaga cagctgtttt tatgtgggca attctatgta aataaagaag tcaatctaga
    53461 aaagctctcc aatctcctgc atgtttgcaa aaccacttta ctcaatgata tcaataacat
    53521 aaaaaaagaa tttgaagaac agatcgctta tacacatcgg gaaaaggacc gttacacttt
    53581 atatttttca gaacatatac ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt
    53641 gtttttaaaa acttgtctgc tttatttgga agaagatgaa cctgattatc ttcaattaac
    53701 tgaatgtgaa ttcatctctg tcagcaaagc ttattcatta aaaaaacaag tattagctta
    53761 ttttaatgac tgcgggatcg aaatcgaccg ctactcccct cgattcactg aaatggaaag
    53821 acgtctgctg cttcttaatg tgtcgtatcg tttaggcggt tttaattctt gggagctacc
    53881 tgaatcgttt tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa atagtggcag
    53941 attttatgat aaagaaaata aagaaatctt atcgattggt tttgctatca gtttcctacg
    54001 ccagcaagtg tgtgctgtga caatagattc aaaatttatc gaagaaatca aaaaacgtcc
    54061 tatttacaat tatgtcgaat cagcatggga aaacactgat ttccaaacct attataaaaa
    54121 agaagaattc gcctttattt tgacactatt caatctttgt aattatggtt tccattctta
    54181 tcagttgatt gcggaagact ttcaacagct acaccaagtg tttattgaca atactcccga
    54241 aataaaagaa ctagttgcta cctttgaatc tcacttcaat caagaattat tcggaaacca
    54301 accgttcgaa cgtgcgctga ttcatctgat gcgttcggct tgggataact atcaattatt
    54361 catgccagaa aaattttatc ttttaaatga ggaacagaca aatttatata aagaagtcca
    54421 gacaattttt tccagctggt ccagccagtt accttatgat ctacggttga atcctaattg
    54481 catgcgggct tttgtgatcg aattgagcgg aattctccgt ctgacaaaag aacatctgac
    54541 tatctatatc gtcacaaatt cggatgttca ttatctgatt tatcgggaag ccttggaagc
    54601 tgtaactact ttcgattttc aagttgcacc aacaatttat tcaagtatat ctgatataaa
    54661 gaagtatgcg cagcagtcct caaaccgggt attatgtgaa cgtacattat atacaccaga
    54721 tgcggtccaa tatgaaaata ttattcctat ttcgatcaat acgatcgatc gagctatcat
    54781 ttcagctgtg caaaacaaat aaggggctgt gacaaaaatc atgtcacagc ctcttatttg
    54841 ttgaagatgc ccatttctgt aacaatctct tgatacacgt caggctcaca acctcactta
    54901 ttttggacat ctacgggtcc gatctcttta tttaaaaaat aagaaatgat ggttcggata
    54961 actaccaacg ccgctagttg gaaaatatcc tgcaaggtag gtttgataat cgattcgatg
    55021 atcccagctg aaacaagaat ttccaaactt agaagtatat agcctcccaa catcttttta
    55081 atagcattgt tctcgctgac tcggtcgctt tgcacttttg tatggacaaa taaatatttc
    55141 aaaaaatcct tgattgatag acaaacaccc caaaccaata aacagatagc aaagatatca
    55201 agtcccagta caagtttttc gaaatatgga agaagaaact gcatcaactc tgtcgtcatc
    55261 ctacctctcc tcttctttgt ttttctttgt cttctatttc ctgattcaag aaataagaaa
    55321 tgactgttcg aatgataata ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga
    55381 taactgactc gatgatatca gcaacgacta gtacttccaa gcttaacaaa atataacttg
    55441 ctagcagttt tcttgtatct gcattacgag tattacgttc tgcatagttt ttcttgacat
    55501 gagaccaatc aaaaagtttg aatagaacaa gaacgatccc gacaattaag ataccgatag
    55561 aacaaatatt taaaatagca atgatccatt ctagaaattc aattaaattt gagcgaaaca
    55621 tatacgctac ctactttctt ttaatttgtt cctattcttt tattgtaaaa gaaagttacc
    55681 ttgttcgtct atttaaaagt tgttctaatg ataacggttc gcttaaattt ttctttttca
    55741 ctctcccatt tttctccctc catccataaa tcgtggaagt atccaaaaca acgtagcaag
    55801 ttaaaataag gcttagtccg tcatcagctt ttcaaagaag cactgtctcg gtgctttttt
    55861 tgtacccaaa tttctaaaaa tagaaccata cacctcctca aatcttttct tcttacctac
    55921 cctattttta tttacaggaa aatcagacac ttcttctttt ttgtcaaaat ttcctttttc
    55981 ttctcgtcct atcatttggc attctaataa aaacgaacta tccttggata tagaaagaga
    56041 aaacaaggag gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt
    56101 gcttgccctt ggcgctgcat atctaggaaa aaagacagga ctattcgaag atgacagtca
    56161 tttatatgat gaattcgaat caatctagca tcctatttag ttgaatcact acggacatac
    56221 ttggaagaca ttggaggaat cacaatgaga aaaagtaaag caataatgat tggtgccggt
    56281 attgccaata tggcagcggc tgtttatttg attcaagaag gcaaatggaa aggggatcag
    56341 atcacctttt attcattgga tgatcacggt tcaaatgatg gtgcaccgac cgacactgta
    56401 gtggatgaat attggaataa aaaccatcca ttagaaaacc aaaaaggcta tattgctcga
    56461 ggcggtcgca tgcttaatta ccgtacctat gtagatttga tggatctgtt aagtcgaata
    56521 ccttctgtta ctgagccagg gatgactgct gaagaagata cgagagattt tgatgcaaaa
    56581 caccgaacct ttgacaaagc acgtttgatg gaaggtggca aaggaatcat tgatggcgga
    56641 aaactgggat tcaataataa agaccgtatt cttctgacta aactgattgc tatgcctgat
    56701 tcagaagaag aaaaactaga caatatcact atcgaagaat atttcaaaga tgatccacac
    56761 ttttttgaaa caaacttctg gtttatgtgg gaaacaacat ttgctttcag aacacgtagt
    56821 tctgctcaag aattgcgtcg ctatatgcat caaatgattt atgaattcac tcaaatcgaa
    56881 catctagtgg gcgtcaatcg aacacgttac aatcaatacg aaagtattat gcttcctttg
    56941 atcgagtact tgaaagaaca aggatgcaaa atcattttga accgccgtgt cactgactgg
    57001 gaattcaaag aaacaccgat gcaagatgaa atcactgtga ctggtttgaa aattaccaat
    57061 acactgactc aagaagaaga atacgttgtg gtcgatgaag atacagcagt tattttcact
    57121 aatgggtcca ttacagattc agctacttta ggagattacg aaacaccagc tgtagaaaat
    57181 atggattatg gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg
    57241 ggaaatcctg acaaattctt tgctgatcga gatgcaagcg aatgggtcag ctttaccctg
    57301 acgacgaaaa accatttgtt gttgaatgaa atcacccgta tcacgactca agaaccagga
    57361 aatgccttga attcatttat ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc
    57421 aatatgtcta tcgtcgtgca tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca
    57481 gttatttggg gttatttcct ttatccaaga cgacgtggcg aatttgtgga taaacagtat
    57541 attaaaatga atggaaaaga aatgctagaa gaactgattg gacaactttc aaaagtagat
    57601 cctggaccag tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt aataaataat
    57661 atccctgtct atatgcctta tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga
    57721 ccaaaagtca ttcctaaaca ttcgaccaat ctggccttta caggagaatt tgtcgagcag
    57781 ccttatcaga tgatctttac tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc
    57841 cattttgcgg gtattcctat gtcccgtctt gtcaaaacac cgcgttacga caaagatatt
    57901 cctactctag ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg tgaaaaagct
    57961 gccctgcatc aagtaataag aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg
    58021 ttggacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttgtgaaaaa
    58081 gctgccctgc atcaagtaat aagaacaccc aagctaaaac tgcttcttat gtttttgact
    58141 tggttggact tattaccgca gatgcaagct tttgaacacc atttaccaag aggttatgaa
    58201 aaagctgccc tgcatcaagt aataagagca gccaagctaa aacagcttct tatgtttttg
    58261 acttggttag acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttat
    58321 gaaaaaggcg tttagtttcg agtatgaaga aaaattttgt gaaaatggtt cctcacgttt
    58381 ttgcataaat ttgacttaat accgagaaac tgatcccttc tgtcataacc tctttttatt
    58441 tatgtctgtt tctttttgaa aacaactatt ttttcaacgt ttactctctt gccaatccaa
    58501 catcatctaa cggatacaac tatttcaatg attaccatta cactaaaaaa tcggaaacac
    58561 acctctccga acacaaatac ataagactat agaatgtttc atttaagaaa aaaagcgttt
    58621 cattatcaaa aaggtaagat ctcctttaaa atgaaattat ctgtagtact aataatgcag
    58681 acaattttat gaatctgaaa ggagcatctc caatgaaagc agtcacttgg caaggaaaac
    58741 agaaaatgga aatcagaaac gtagatgatc caaaaatatt agaaccaaca gatgcaatca
    58801 tccgtatcac agcaacagcc atttgcggct ccgatctgca tctttaccat catggtgaag
    58861 cagttttgga aaaagggtat gttgtaggac acgaaccaat gggaatcgtt gaagaagtgg
    58921 gatctcaagt tcaaacattg aaaaaagggc aacgtgtggt tatccctttt aatattagtt
    58981 gtggtcattg tcatttctgc ctgaatcaca tggaaagcca atgtgatgaa tcaaatccag
    59041 atcagttata tggcgggctt ttcggatttg gcaagttgaa cggtaatcat tggggtggtc
    59101 aagcggaata tctaagagtt ccttatgcag actctacttc atttgttgta ccagataatg
    59161 atcttcctga tgaaaaagtt ttatttctat cagatatttt gcccacagct tattggagcg
    59221 ttgaaaacgc aggagtaaaa aaaggcgata cagttgtgat tctaggttct ggtccagtcg
    59281 gtctattcgc tcaaaaattc gctgttatgg ctggagcaga gcgcgtaatc gcagtagatc
    59341 cagtacagca ccgtctagat aaagcggcta attacaatgg ggttgaaact tatttcttag
    59401 aagacacggc acaagctgga acagaattgt atgaactggc taaaggtggc gcagatgtca
    59461 ttatcgattg tgtaggaatg gacggattag agcctgtcaa agaaaaagcg aaaaatttag
    59521 taagcttaca aagcggcaca atctctccat tacagatggc atcgcaagct gtcaaaaaat
    59581 ttggaacagt ccaagtaact ggtgtttata tgactccagc ttcttcttac ccgctgcaag
    59641 aattttttat gcgggatatc gctgtcaagc atggacaggc accaattatc catctaatgc
    59701 caaaaattta cgcaatgatt gcagaaggac tattcgatcc tacacaaatc attactcatt
    59761 cgctgccact atcagaagca gcacaagcat atgagatctt tgacaaaaaa gaagataaaa
    59821 atataaaagt tgttttgaaa ccttgaattc agtaaaaaat acaggtgatt ttctttatgc
    59881 ccaaaatcaa acgaaatacc catgagatcg aaataggaaa agaagaagat atcgaaatac
    59941 gaggacgtcg cttccgcttc tatttccaaa atcgttacac attgatctca ctggctgtcg
    60001 atttattaac cgggattttc tatatttttg gaagccttgc ttccttaact aaaatccccg
    60061 atcgatatgg cacctatttt tatttggctg gtgctatttt cttgacgatt cgtcctattc
    60121 tcagaatcgt aaggaacatt tttatttacg acgaaaaaaa agcaatgaag agaacaagca
    60181 aaaaaaaaga ggcatgagaa tttttcaaat ctcatgcctc ttttttcacc ctatcgtatc
    60241 actttgatac agacgagcgg tcgtcccact ttcttccttg accattgtca aaaatgtcca
    60301 ctcgtatcgt tcataaaacg tggtatgatc tgtaattaag taaagttttt cgtacccaag
    60361 ttgatgcatt tcttttctga tttccgctaa tatccgcttg gctatcccct gcttacgctt
    60421 ttccggttca acaaacaggg cacaaagatt tggtgtcaga tcagggcggt tatggaagtc
    60481 gttttcaatg acgcccgctc ctccgacgat ggtttctttt tcatctaaga ccacatacca
    60541 ttgaggaata ctttccttat tagcgattcc agtacgaata ctttcctcat acacaacagc
    60601 aggaactccc cattttgatg aaaaccaatt cgcgctttgt tttactaatt ctgggtgttc
    60661 tcttaatttg atccagttat atgtaaccaa tgtttaaacc tccgactaaa ataaaacgtt
    60721 tacaaaaatt atacaagtct ttatgaaaat tagcaatgac aacaatagtg atgtaaaaaa
    60781 aaaacggaaa acaatcactt gttctccgtt ttttgattaa tcaagcatct aaagcttctt
    60841 ttcttctttc ttggatcgta gctaccttga cgaacggtag atagatcaaa atagaaagaa
    60901 ccaaattgac agcggctagt acaccacctg caatactttt tgttgccaat acaccgccaa
    60961 taattggtgg agttacccat ggaggcataa cggtagctgc tggcactaaa cctgtagaag
    61021 tcgcaaagta agcaacacta actaagacca ttggtgtcaa aataaatgga atgaacatga
    61081 tcgggttcaa cacgataggt aacccaaaca tcgttggttc attaatattg aagacacctg
    61141 gcgcaatcga caagtttgtt acaaccatat atggtttgtt tttacgtcct actaaataaa
    61201 tagctaacag taaacctaaa gttgcacctg ttcctcctaa gttgacaaag ctgtcaaaga
    61261 aaggtttgtt cacgataaac ggtgcgctat gcccagctgc gattgcatta acgtttgctt
    61321 caatggctgg cgcattgatt gtttgcatca aaggatcgac catattcgct ccgtgtaaac
    61381 caaagaacca taagaatggt gtgataaatg caagcagtaa ggcagatgga taagagtttg
    61441 caagtcccat aaatggttct tggaccactg tatagaaaga acctacgata tcatcaacac
    61501 cgaatgcagc aaaaattgca gcaactaaag caaacgcaga gatagtgatc attgctggta
    61561 gtaatgaagc aaatgctttt gcaactgccg gtggcacacc atcaggcatt ttgatgatca
    61621 atttttcatt tccgcttaaa cgtacaaaga tttctgttga aataatccca acaataatag
    61681 caataaacaa accgtttgag ctcataccta atgctccgcc taaagcgaag aaagagccta
    61741 aagaaatcac acctgcggaa acaccgtctt ttcggtagcc gttagctaaa tgataagcaa
    61801 gcaagcaaga aagcaatcaa gacagaaaga atggcaaatg taccgttcca agcagcagcc
    61861 ccgaatcctt tccatgattc accaccaaaa attgagttca tcaattcttg gaacccggga
    61921 atcggtaagt tattgatcat tactgctaat gctcctaaga tcattaacgg catactcacc
    61981 ataaatgagt cccgaatcgc aactaaatgc cgttgtgcac caatttttga tgctacaggt
    62041 ataaaatgtt tctccataaa tgcaacaaat ttatccataa atcttccctc ttttctttac
    62101 tgtagattac gcgttcaatc gacgatacag atcaatgatc tcttttgcta aatctgtaaa
    62161 agctatagat gtcatcagat gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg
    62221 atttccttgt gcttcttgtg tcagcatctc tgtttgagag tgatgtgctt ggactaaaga
    62281 ttcctctgca tctttgattt tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc
    62341 aatcgcttcc attgcatcgc tttttgcatt tccgccaaac atgatcagac ccatgatggc
    62401 ttctagattt ttttgatctt ccatttattg tatcctccga taatttttat tatttgttta
    62461 ttagtttttc tgcttggtcg agtacttttt caccattcat catgccatag tcagccatat
    62521 tgattacatc taaaggaatg cctttaggtg ctagcttttc agcaaattga ttttgcataa
    62581 agcgtacttg cggtcctaaa agtaaaacgt ctacattttt agattctagt tgattatccg
    62641 catctgaagc agatacagca aagatgtctg cttccattcc ttttgcttct gctgcctttt
    62701 gcatttttgt gactaggaga cttgtactca ttccagctga acatacaagc atgattgttt
    62761 tcttttccat tttaaacacg ttcctttcta tacttacact tgtatataag caagtagcgt
    62821 gccaacttta aaagcgcttt aaaaagttga tatcatcaca tttaaggtct ataccaagtt
    62881 gcatacacac taaaaaagaa agcgcacact aaacacacaa attaattttt attacctttt
    62941 ttcttttaac gtgcacaaaa aaagttgtga cacaaaactt gtcacaactt ttttcaaata
    63001 aacggtattc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataagat
    63061 gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca caacctctca
    63121 ataaacggtg ttcaatcagc tgaccctggg tattagctca aaacgctgga ttcacaacct
    63181 cttttatttc aaataaccag taaatacaat gaacatctac aaaaaacttc tatttattta
    63241 tagatttcgt caggcaaatc caattctctc ttatcaatca aagtctgata ccacgaagca
    63301 gattttttca atgttctgct tcgttcatcg ttcaattcga ttcgaactaa tccatatcga
    63361 ttcttaaatg cattcattgg tgacacacag tcagtgaatg cccataacat atagccttcg
    63421 caatttgcgc cttcttctgt tgctttgatc aagtaatgca aatgctgtcc gataaattcg
    63481 atacggtaat catcttgcac gattcctttc tcatccatat actgttcttc atattctctt
    63541 cccataccgt tttctgttat cagccaaggg atattatgat aattttcttt tagatacatg
    63601 gctatatcat acatgatttt cggatagatt tcccatcccc tggaattatt cattttcttc
    63661 ccaggtaaat caaagacttc ataatatcgt tcaggatgga aaggtgtctc ttcattccat
    63721 tgataacgag gggcgcgaac gcgtttagga taatattgat tgattccaag gaaatctact
    63781 gtatgatgct gaatcgtttc taaatcctct gttgcaggat cgaaataaat cgattgtttt
    63841 tgacacaact ccaggaactc ttctgtgtag tatcctttta ccatcggatc aaaaaatact
    63901 cgattataaa acaaatcata catataagca gctttttggt cttctggaga attggatcga
    63961 gcatagacca tttcaggatt taaaatacag ccaatccttc caccataatt tccgctgcgg
    64021 taaatctcta ctgctttagc cgttgctaaa actttatgat aattccacat catccacttt
    64081 tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg
    64141 attggttcgt tgaatgtaaa ccagtttttt accctatcac cgtaacgatc aaaggcaatt
    64201 tgagcatact tagcataaaa atcaactact tttcttgaag accagccatc atatttttcc
    64261 aaaagaaaag ctgggacttc ataatgttcc aaacaaatca tcggttctac gtctgcttcg
    64321 atcaaagagt cgatcatttc acttatatgc tgagcatatt cttcatctac tatacagttc
    64381 tcataatcag taaaaaaacg agcccaattg atagaagtcc gataatgtgt cagatggatg
    64441 gatttcatca gatccacatc ctgctggtat tgttccataa aattagttgc tacggctggg
    64501 ccatacccat tatgccaaac aaaatgttca tttttatacc agctatctat aaacgaatcc
    64561 tgtccttctt tttttccttt ccaaccttcc gtttgccaag cagaagatgc agcaccaaga
    64621 ataaaatttt ttggaatagt aattttcatg atttcctcct gttttttaga tcccatcatt
    64681 ttccacacga tttgctactt ttacaaatgg cagatagatc aaaatagaaa caataataca
    64741 aacaagttgc gtaataactg caccgatatt tcctgctgtt gccagataag cattgacgat
    64801 cggcggcatt ggccaaggga ccattacaac tgctttcgcc gcaaatccta aagcggttgc
    64861 aaaataacca atcgttccag tgataagagg cgttaagata aacggaatag ctaaaattgg
    64921 attcagcatg atcggcatac caaaaataac tggttcattg atgttgaaga tcccaggagc
    64981 aagggataac ttggtgattt gtttcatatc ttcccgtttt ccaactaaca atactgcaat
    65041 cagtaaaccg atcgtgactc cagacccgcc catactcata tacatatccc aaaatggcat
    65101 agtaataatg ttcggtactt cttttccagc ttcaaaagct tccgtattta cagcaattgc
    65161 cgctaaaagc aacggttccc gtatcggctt caccatttga tttccatgga taccaatcac
    65221 ccaaaagact tgcgcaataa acatcaatag caaaataccc ggtaaccctt gtacgacacc
    65281 ttctaatggg cgttgtacga cattgtaaat gatatcataa aggtacattc ctgtaagttc
    65341 tttgatgata aatcccgcag ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc
    65401 agaaaaacta gcagaaacat tcgttggcac ggtatcaggc attctcagct gtagtttctt
    65461 ctgtttcctc aaccagcaat aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa
    65521 taaccctttc gtatctgtat attgtttagc caatacgttt tctactataa cagtctgatt
    65581 ttcattcact aattcataaa cagttggatt aacagataaa taagacatca ctgcgatgat
    65641 accagggaat acaccatcca ctttattcag cttggctaat tcgatcccta tcagaaaaac
    65701 agcatagatg gtcaagaaac ttagtgtgac atagctgatg gcagatgcga taggctttaa
    65761 ttcttctaaa aaacgcaaga aacttatctg tgctaagcca ttttccgagt caaagaccat
    65821 tgctgaaaac aatgtgccaa aagcacccgt aataattacc ggaatcaatg cagcaaatgc
    65881 gttttttatc accatgatat accttaaatt attgattctc gttgccacgt tccctaaaac
    65941 attggctaat ttatccataa aactcattcc agatcccctc attcctgctt cttctccttg
    66001 acgtcatctc tttttgtcta ttagtcagcg cttacatttc gatgaacaga aactatttac
    66061 tcctcttttt taccaaatat aaaacaaaaa ttcatctgat gtatttatca taaaatacat
    66121 cagatgaact gtcaattagt ttctttttct cctattattt tctgtaattc tctccgattt
    66181 attgccatat ggacagtcat tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc
    66241 tgaacgatct ctgtatgaat atcgatcgtc tcgattttca gatgctggat atcataattt
    66301 ttattaaaca aagcaattga ttcattaatg attgggataa tatgcagcaa ggctacgttt
    66361 ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg
    66421 ccttctcgaa aagaagtttc aatcttttgc gttaattttt tcatttggac gatttgattc
    66481 ttcgttgcat attccgctgc caaacctgcc atcttaggct ccaataaata tctcagttca
    66541 aataagtctc gtatcatccg atcctgatct tcaatcaaag aaaaaccgag tgggtcattg
    66601 gcaatccctc gcttatcact gacaaatgtc cccgctcctt gccgaacttc aaggatattc
    66661 ctggaagcta gagcccgcac agcttctcgc accgtgcttc gccccacctc taaataatcg
    66721 gacaattcat attcattcgg cagtttatct cctacttgca actgcatttc ctttaaatat
    66781 ctaaggatct tgtcagtagt tacttctact agggttttat tttcttccaa accacatacc
    66841 tccttttcat ctgatgtatt ttttcgtaat tattataaca cgtttgaatc atctgatgat
    66901 tttttgtata caaaattgat taatccgttc atagacatac aaacgaagaa gatatttacc
    66961 ataaaaaaga ctaggataaa attcactcct agccttcgat catttttatt ttgaacgaca
    67021 aaaaatatga tgttcaataa cagacgatct tcattcgatt cttgatgtca tcttttgttt
    67081 tttttaatat atggataaaa agtttgatta catcgtttct attctagaat ttggtcgatt
    67141 ctcaattctt cttctggtga gaaatctaaa ttatccatca ttcgaacact ttcttgtaac
    67201 tgacttaacc gacttgctcc gaccagtaca cttgtaacag atttttgtcg caaattccat
    67261 gcaagggcca tttgcgctaa tgattgtcct cgtgatacgg cgattgtttg cagtgctttg
    67321 attttttcca gagttgatcc aacttgttct tctgaaagga atggtatttc cttacgatgt
    67381 gctctagaat cttcaggaat accatgtaga taacgatttg tcagcaaccc ttgagcaagc
    67441 ggactgaacg tgattgcccc tagcccttct tcttctaata ctggagatag atcgtcttct
    67501 agtagtgcac gagaaaacat attgtagcgc atctgatgaa taatgaacgg tgcttccttt
    67561 ctttttaaaa tttcagtcat tttttttgta tcttctccat tatagttgga tataccaata
    67621 tataatgctt ttccttgtct tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt
    67681 gtatcagggt ctgggcgatg atgatagaag atgtcaacat aatctagccc cattctttgg
    67741 agactttggt cacaacttga aatgatggat ttacgagagc cccattcccc atatggccct
    67801 tcccacatat gataacctgc tttgctagaa ataatcagtt catcacggta ggcttgaaaa
    67861 tcttctctaa aaatacgccc aaagtttttc tctgcagctc cagcagggcc tccataatta
    67921 ttcgcaaggt cgaaatgggt aattcccatg tcgaatgcgc ctcttaaaat ctctctttga
    67981 ttatggatag gatcataatc accaaaattt tgccataatc cgagagataa aatcggtaat
    68041 ttcaaacctg aatgaccgga tttacgatat tccatttttt cataacgtgc actatctgca
    68101 atatacaatt tttctcctcc tcatcaaatc tttttaaaac gctttctttt ttctattttt
    68161 agtttactac attttaaaga aaaggagtta atcaaaatga ttttttcgta tatttttttg
    68221 ccatttgttt tttactcgtt gatagatcag aaattgatta cgtgcttgct ggattttttt
    68281 ataacgtttc ggttgttttt tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt
    68341 accggctggt tcgatttttg tgacgattgg ttggtggaaa gttcctgaac tgtctagttt
    68401 ctgttttgat tgttccgcta attctttttg tcgttcgcct tgataaaaaa tcactggacg
    68461 atattgcttc cctctatctt gaaattgtcc aaatgtatct gtgggatcag taatttgcca
    68521 atagattgtc agcaactcgc tataacttat ttctcttgtg tcataaatga tctccacagc
    68581 ttccacatga cctgtgtaac caccacttac ttgatcataa tttggttttt caacatgtcc
    68641 gcccgtgtat cctgacaaaa cagataggat cccttttttc gattcaaacg gctcgaccat
    68701 acaccagaaa catccccctg caaatactgc acgttcttga tagtcagcat cttgcatggc
    68761 atgcttggaa aagtcatatt tttgctcctt atcaaatcct tccgtaatca ataaataaaa
    68821 gtccattaca tcaggcgtca gattcattcg gatagctaat ggacgtagtg ttgcttctag
    68881 ttttgataac tgttctttaa cggatatatt ttctcttatg tcattctttg tctgaatcaa
    68941 tacttttcgt tcccaatctc gtgtattcgg attcaaaatc agattataaa gtgtttgaat
    69001 gaatatttgt tcctcatcca tagattctcc tcctttccga agaagacaag ctagtgactt
    69061 tagctgtaat ttatcataaa gtgaggaaat aagcgattac ttcatccccc tacagatttt
    69121 tcgtataaaa aagagagaat ccttatcgac agattctcct ttttaatcaa tttattttat
    69181 aaatgaaaag ttaatttacc ttttatggaa aactatttta aaaaataaac ataaatatgc
    69241 agttaatgta acaagataag aaataatagt cgttactgta taacttgtgc ttactttaat
    69301 atcagcaact cctttgactc cgcttatttg taccaatcca ttagcattca cctgctcctt
    69361 tacaggttga tgatttattt ctacattcgt tcctaaataa gcatatacag gaagtgtgat
    69421 tacttggctc ttctcattag ttactctata tatgatagaa ctaccagttg ttttataagg
    69481 aaggtttacc cattgactgc caatctttgc ttcgtgtttt attatatgct ccttattctc
    69541 caatgaagcg ataggagaat aatcatataa aaaataatcc attcctttag tggtatacgc
    69601 ctctactgtt tctttgttta caacaatatg tcccggctga gaaattattt tttgattgaa
    69661 atttttttta ctagaaacag ttaaagtgat taataacaaa acagtcataa ataaataaaa
    69721 tagttctctt gtcttattgt atcttctatt tttgaaaaat atcatcgtta atatcagaga
    69781 agagaatatt atttgcaagc ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt
    69841 attttgcaga agataccatg gcaccaaact agttgtagct agccaagtca aacacgtacc
    69901 ataataaatc tttttttcta tgtttgttaa ttttttaaat aaaatcaggg gaatacataa
    69961 agataacaga ccaactagtc ccattgagta agtagttaag tcgttgttca tagcttcagt
    70021 cagactatcg aacacattag atgcatgagc atataaattt gtcacgctag gtttgttgat
    70081 tttttggtag agcatttgtt caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt
    70141 cattcctgcc gcactaaata attttttaaa tatagcaata aaatctgcat tcttgaaacc
    70201 tttagaaacc aaacgaataa aaataaaaat gataattaat gctgtactca ttaatgttga
    70261 tagtacatgg ctgtacacta gcaaactcat actcaatgct agctttacag acggatactt
    70321 ttcttctttt ccagagatga tttgataagc ataaaataat acagggataa caagtgttat
    70381 tgcgatagct tcggcaattg cggaacgata gtagatgtct actgtccgat aagcagagaa
    70441 aatatagaga caactaaata aaaatgccgc ttttttttgt tttaaaaacc gctcgccata
    70501 atgataagct acgagtgttg tacacaagtt caataaccaa acgtaaataa tatagcttac
    70561 aatcaaattt tttgtcatcc aatataacat tacagctgga aaaaaagtca gaaagggata
    70621 aaaataattt acaccatatc cagtataatt gaatgtctca taattaatcg gactggtaaa
    70681 aatagtcgat aaccccttga ttcgagataa gtgaaaagaa agatctccag tggtataaat
    70741 actctgtgta tttttgaaaa agatgaaaga atatatacta ctaactataa ttaatccact
    70801 caatattact acttttttat ttattttcat tcttcttgtt tatctccttt ttagtcacat
    70861 atcgagaatc tttcaactta ttaggtgcaa atcgtttaaa acttgaatac accactaaac
    70921 aataagtcag aatactgatc cataaagcca tgagagcagt tggagtatat tttgttgtaa
    70981 tttctatctt attttctcca gcatttacat ccacattcac taagccatgt tttttataag
    71041 agtcatttac gactttaccg tttactttta cctgtgtacc taaataagca tatactggta
    71101 ctgtcacttt ttgctttttg ttgcttgcca ctgtgtaagt aatgttatta tctgcagctt
    71161 tatacttccc tttttcccac gattctccta cttttacttc atgtttttct aagtgaggcc
    71221 tttcttttat cgcttctgtt ggggcgtaat cgtataatcc tcccatatct gatgttgtat
    71281 aacgtgggag tgtttcttca ttgataacaa tatgaccgtg ttttgattgg ataacttttg
    71341 cgtaattttc ttttgctgct atagacgtga cgagtagaaa caccacagta gcgccaatga
    71401 ttaaccatga atatttttta ttcatagtct ttttcataaa aacaagcgtt atgatcattg
    71461 aagaaaaaaa gatttggata cctaagatac gccaaggaaa ctggagcaag cttatcggtg
    71521 ttttttgaaa caaaaaccat ggaaataaat tagttacgca tacccatgta gaaactacgc
    71581 caacatagat gattttttct tttttctcca tttctttcag tatgaataaa ggaaaaatca
    71641 acgcaaccaa tcctaataac cccatagtgt atgttgtaat atcgttattt aacgcttgaa
    71701 tgatactatc tgaaatagaa agtgcttcta gctgcaaatc tgttttgaag ggactgtgta
    71761 tttcttgata attcatctgc tgcaacatag gataccaaaa atagctagtt aatgcacaag
    71821 tcaaaaaaac agcctttact aaattcacga ccacaaattt tatatcgttt tttatttgct
    71881 tgcttaaaaa caaacgaact ataatcatac aagcaatcaa tgcactacac aatagtgtag
    71941 ttaagacatg agtatacaca agtagactta tactgacaga taaagcgatg actttttttt
    72001 ctctattgaa tatcaatcca taagcataat ataaaacagc tgggataatc gtaagagcga
    72061 ttgcttctgc aattgcggat cgataataga tatcaactgt tcgataagtt gcaaaagtat
    72121 ataaacaact aaataaaaaa gcagcatcta tctttttaaa aaacttttct ccataataaa
    72181 aa
//
LOCUS       DACEOM010000003        67604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEOM010000003 DACEOM010000000
VERSION     DACEOM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.003"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(54..1220)
                     /gene="dnaJ"
                     /locus_tag="IW781_000144"
     CDS             complement(54..1220)
                     /gene="dnaJ"
                     /locus_tag="IW781_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1181168.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(1371..3200)
                     /gene="dnaK"
                     /locus_tag="IW781_000145"
     CDS             complement(1371..3200)
                     /gene="dnaK"
                     /locus_tag="IW781_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1181169.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(3251..3814)
                     /gene="grpE"
                     /locus_tag="IW781_000146"
     CDS             complement(3251..3814)
                     /gene="grpE"
                     /locus_tag="IW781_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1181170.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(3908..4951)
                     /gene="hrcA"
                     /locus_tag="IW781_000147"
     CDS             complement(3908..4951)
                     /gene="hrcA"
                     /locus_tag="IW781_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1181171.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(5185..6351)
                     /locus_tag="IW781_000148"
     CDS             complement(5185..6351)
                     /locus_tag="IW781_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1181172.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(6367..6594)
                     /locus_tag="IW781_000149"
     CDS             complement(6367..6594)
                     /locus_tag="IW781_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1181173.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            7078..7668
                     /locus_tag="IW781_000150"
     CDS             7078..7668
                     /locus_tag="IW781_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1181174.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7858..8571
                     /locus_tag="IW781_000151"
     CDS             7858..8571
                     /locus_tag="IW781_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181175.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(8686..9696)
                     /locus_tag="IW781_000152"
     CDS             complement(8686..9696)
                     /locus_tag="IW781_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1181176.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(9733..10215)
                     /locus_tag="IW781_000153"
     CDS             complement(9733..10215)
                     /locus_tag="IW781_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1181177.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            10365..10733
                     /locus_tag="IW781_000154"
     CDS             10365..10733
                     /locus_tag="IW781_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1181178.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            11198..11650
                     /locus_tag="IW781_000155"
     CDS             11198..11650
                     /locus_tag="IW781_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1181179.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(11774..12625)
                     /locus_tag="IW781_000156"
     CDS             complement(11774..12625)
                     /locus_tag="IW781_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1181180.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(12638..13423)
                     /locus_tag="IW781_000157"
     CDS             complement(12638..13423)
                     /locus_tag="IW781_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1181181.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(13775..14716)
                     /gene="ribF"
                     /locus_tag="IW781_000158"
     CDS             complement(13775..14716)
                     /gene="ribF"
                     /locus_tag="IW781_000158"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1181182.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(14720..15643)
                     /gene="truB"
                     /locus_tag="IW781_000159"
     CDS             complement(14720..15643)
                     /gene="truB"
                     /locus_tag="IW781_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1181183.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(16011..18373)
                     /locus_tag="IW781_000160"
                     /pseudo
     CDS             complement(16011..18373)
                     /locus_tag="IW781_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18821..18994
                     /locus_tag="IW781_000161"
     CDS             18821..18994
                     /locus_tag="IW781_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1181184.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(19151..19498)
                     /gene="rbfA"
                     /locus_tag="IW781_000162"
     CDS             complement(19151..19498)
                     /gene="rbfA"
                     /locus_tag="IW781_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1181185.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(19525..21831)
                     /gene="infB"
                     /locus_tag="IW781_000163"
     CDS             complement(19525..21831)
                     /gene="infB"
                     /locus_tag="IW781_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1181186.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(21844..22155)
                     /locus_tag="IW781_000164"
     CDS             complement(21844..22155)
                     /locus_tag="IW781_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1181187.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(22152..22445)
                     /locus_tag="IW781_000165"
     CDS             complement(22152..22445)
                     /locus_tag="IW781_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1181188.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(22467..23642)
                     /gene="nusA"
                     /locus_tag="IW781_000166"
     CDS             complement(22467..23642)
                     /gene="nusA"
                     /locus_tag="IW781_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1181189.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(23665..24138)
                     /gene="rimP"
                     /locus_tag="IW781_000167"
     CDS             complement(23665..24138)
                     /gene="rimP"
                     /locus_tag="IW781_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1181190.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(24283..28641)
                     /locus_tag="IW781_000168"
     CDS             complement(24283..28641)
                     /locus_tag="IW781_000168"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1181191.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(28853..30562)
                     /locus_tag="IW781_000169"
     CDS             complement(28853..30562)
                     /locus_tag="IW781_000169"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1181192.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(30630..31898)
                     /gene="rseP"
                     /locus_tag="IW781_000170"
     CDS             complement(30630..31898)
                     /gene="rseP"
                     /locus_tag="IW781_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1181193.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(32059..32859)
                     /locus_tag="IW781_000171"
     CDS             complement(32059..32859)
                     /locus_tag="IW781_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1181194.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(32856..33668)
                     /locus_tag="IW781_000172"
     CDS             complement(32856..33668)
                     /locus_tag="IW781_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1181195.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(33863..34420)
                     /gene="frr"
                     /locus_tag="IW781_000173"
     CDS             complement(33863..34420)
                     /gene="frr"
                     /locus_tag="IW781_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1181196.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(34423..35145)
                     /locus_tag="IW781_000174"
     CDS             complement(34423..35145)
                     /locus_tag="IW781_000174"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1181197.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(35281..36162)
                     /locus_tag="IW781_000175"
     CDS             complement(35281..36162)
                     /locus_tag="IW781_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1181198.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(36261..37043)
                     /gene="rpsB"
                     /locus_tag="IW781_000176"
     CDS             complement(36261..37043)
                     /gene="rpsB"
                     /locus_tag="IW781_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1181199.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            37402..37881
                     /locus_tag="IW781_000177"
     CDS             37402..37881
                     /locus_tag="IW781_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1181200.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            38098..39189
                     /gene="phnW"
                     /locus_tag="IW781_000178"
     CDS             38098..39189
                     /gene="phnW"
                     /locus_tag="IW781_000178"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1181201.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            39182..39310
                     /locus_tag="IW781_000179"
     CDS             39182..39310
                     /locus_tag="IW781_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1181202.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            39314..39859
                     /locus_tag="IW781_000180"
     CDS             39314..39859
                     /locus_tag="IW781_000180"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1181203.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(40313..42004)
                     /locus_tag="IW781_000181"
     CDS             complement(40313..42004)
                     /locus_tag="IW781_000181"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1181204.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(42424..43371)
                     /gene="arcC"
                     /locus_tag="IW781_000182"
     CDS             complement(42424..43371)
                     /gene="arcC"
                     /locus_tag="IW781_000182"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1181205.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(43486..44505)
                     /gene="argF"
                     /locus_tag="IW781_000183"
     CDS             complement(43486..44505)
                     /gene="argF"
                     /locus_tag="IW781_000183"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1181206.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(44596..45825)
                     /gene="arcA"
                     /locus_tag="IW781_000184"
     CDS             complement(44596..45825)
                     /gene="arcA"
                     /locus_tag="IW781_000184"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1181207.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            46286..46987
                     /locus_tag="IW781_000185"
     CDS             46286..46987
                     /locus_tag="IW781_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1181208.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(47596..48612)
                     /gene="tsaD"
                     /locus_tag="IW781_000186"
     CDS             complement(47596..48612)
                     /gene="tsaD"
                     /locus_tag="IW781_000186"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1181209.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(48609..49073)
                     /gene="rimI"
                     /locus_tag="IW781_000187"
     CDS             complement(48609..49073)
                     /gene="rimI"
                     /locus_tag="IW781_000187"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1181210.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(49080..49622)
                     /gene="rimI"
                     /locus_tag="IW781_000188"
     CDS             complement(49080..49622)
                     /gene="rimI"
                     /locus_tag="IW781_000188"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1181211.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(49606..50430)
                     /gene="tsaB"
                     /locus_tag="IW781_000189"
     CDS             complement(49606..50430)
                     /gene="tsaB"
                     /locus_tag="IW781_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1181212.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(50519..51499)
                     /locus_tag="IW781_000190"
     CDS             complement(50519..51499)
                     /locus_tag="IW781_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1181213.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(51523..53007)
                     /gene="galT"
                     /locus_tag="IW781_000191"
     CDS             complement(51523..53007)
                     /gene="galT"
                     /locus_tag="IW781_000191"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1181214.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(53019..54008)
                     /gene="galE"
                     /locus_tag="IW781_000192"
     CDS             complement(53019..54008)
                     /gene="galE"
                     /locus_tag="IW781_000192"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1181215.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            54256..54423
                     /locus_tag="IW781_000193"
     CDS             54256..54423
                     /locus_tag="IW781_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1181216.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(54485..56296)
                     /locus_tag="IW781_000194"
     CDS             complement(54485..56296)
                     /locus_tag="IW781_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1181217.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(56293..56658)
                     /locus_tag="IW781_000195"
     CDS             complement(56293..56658)
                     /locus_tag="IW781_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1181218.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(56821..57216)
                     /locus_tag="IW781_000196"
     CDS             complement(56821..57216)
                     /locus_tag="IW781_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1181219.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(57234..58196)
                     /locus_tag="IW781_000197"
     CDS             complement(57234..58196)
                     /locus_tag="IW781_000197"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1181220.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(58196..58408)
                     /locus_tag="IW781_000198"
     CDS             complement(58196..58408)
                     /locus_tag="IW781_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1181221.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(58429..59127)
                     /locus_tag="IW781_000199"
     CDS             complement(58429..59127)
                     /locus_tag="IW781_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1181222.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(59147..59689)
                     /locus_tag="IW781_000200"
     CDS             complement(59147..59689)
                     /locus_tag="IW781_000200"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1181223.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(59821..60825)
                     /locus_tag="IW781_000201"
     CDS             complement(59821..60825)
                     /locus_tag="IW781_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1181224.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(60822..61811)
                     /locus_tag="IW781_000202"
     CDS             complement(60822..61811)
                     /locus_tag="IW781_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1181225.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(61808..62614)
                     /locus_tag="IW781_000203"
     CDS             complement(61808..62614)
                     /locus_tag="IW781_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181226.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            62780..63736
                     /locus_tag="IW781_000204"
     CDS             62780..63736
                     /locus_tag="IW781_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1181227.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(63813..64331)
                     /locus_tag="IW781_000205"
     CDS             complement(63813..64331)
                     /locus_tag="IW781_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1181228.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(64419..64568)
                     /gene="rpmG"
                     /locus_tag="IW781_000206"
     CDS             complement(64419..64568)
                     /gene="rpmG"
                     /locus_tag="IW781_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1181229.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            64796..65242
                     /locus_tag="IW781_000207"
     CDS             64796..65242
                     /locus_tag="IW781_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1181230.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(65437..67332)
                     /gene="asnB"
                     /locus_tag="IW781_000208"
     CDS             complement(65437..67332)
                     /gene="asnB"
                     /locus_tag="IW781_000208"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1181231.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        19041 a        14377 c        11737 g        22449 t
ORIGIN      
        1 gattgttcgt ctggttttta attattaatg acttagcgtt tttactagaa aaactacttc
       61 cgctttttcc cgccaaaagc atctttaaac ttatcgaaga aaccgtcttc ttgctgttct
      121 tcaactttga tgccgccagc ttcagcgaat gcacgtaatg cttctctttg ttgttcattt
      181 agatttttcg gtgtgatcag ttttactttg acatgttggt cgcctgttgc accaccacgt
      241 aacttcggag cgccttttcc acgtaaacgg aagttcgtac ctgtctgtgt tccagcaggg
      301 atcttcaatt tcacatctcc atggacagtt ggtacttgga cttcatcccc caaagctgct
      361 tgtacaaaac ttaaaggcaa ttcatagtaa atttcagaac cttcacgatc aaagatatca
      421 ctttcttcta cacggaagac cacatacaaa tcaccaaatg gtccgccgtt ttctccagct
      481 tctccttgac cagctaaacg catttgttgt ccttcttcta caccagctgg tacattcact
      541 ttaactgaat gtgctttctt ctcatgtcct gagccatgac aggttggaca aggttctttg
      601 atttctttcc ctgtcccatg acaaacatca caagtttgac ggctcatcac tcgtccaagt
      661 ggtgtttggc gttctacatt aatcgtacct gcaccatgac atttatgaca agtagttggt
      721 tgtgtgcctg gttttgcacc gttcccacca caagtatgac agatttcttc ccgattatac
      781 ttgatttctt tttctacacc aaaaatcgct tcttcaaaag acaagtcgac cgtatattgc
      841 aaatcagcac cttgacgagg cgcatttgga tcgactgacc gaccgcctcc tccaaagaaa
      901 gagtcgaaga tatcttcaaa accaccgaag ccgccaccag agaagccgcc gaatccacca
      961 aatccgccac cagctccacc agcaccgtaa tttggatctg tgcctgcatg cccgtattgg
     1021 tcatatgcag cgcgtttttg cgggtcactt aaaatctcat atgcttctga tacttctttg
     1081 aatttttctt ccgcatcagg ttctttattg atgtcaggat ggtatttttt cgaaagctta
     1141 cgataagctt tctttatttc atcttctgat gctccttttg agagccctaa gacttcataa
     1201 taatcacgtt ttgttgccat aggctttcct cctattggtt ctcatcattt tcacgtcgct
     1261 tgttatcata tcatattttg tttgaaacaa aaaccgatac ttcaaactat ttttcaaaag
     1321 aaaaagccaa agtagcaact ttggccttct ctcgaaattc agtgaactga ttatttatcg
     1381 tcgccatcga cttcttcaaa atcagcatca acgacatcat ctgctccgcc ttgagctgct
     1441 tctgggtttt cttgagcttg ttgttgtgcc gcttgttcgt atagtttaac agtcaagttt
     1501 tgaacgattt cgttcaatgc atcgcgttta gttttcattt cttcgatgtt gtttgcttca
     1561 acagcggctt tcaattcatc gcgagctgtt tctgcttttt tcacttcttc ttcgtccact
     1621 ttgccttcta attctttcaa tgttttgtcc acagagaata gcaatgcatc tacatcatta
     1681 cgtaggtcaa cttcctcttt acgagcttta tctgcttctg catttgcttc tgcatctttt
     1741 accatacgtt cgatttcttc gtctgttaga cctgaagaag atttgatcgt gattttttgt
     1801 tctttttgtg tacctagatc tttcgcagat acatttacaa tcccgttttt atcaatatcg
     1861 aatgttactt cgatttgtgg aataccacga ggagctgctg gaatatctgt caattggaat
     1921 cttcctagag ttttgttgtc agcagccatt gggcgttcac cttgtaatac atggatgtct
     1981 actgctggtt gattatctgc cgcagttgag aatacttgtg atttacttgt tggaatcgtt
     2041 gtattgcggt caatcaattt agtgaacact ccacccattg tttcgatacc taatgacaat
     2101 ggtgtaacgt caagtaaaac aacatctttc acatcaccag tgatcacgcc accttggata
     2161 gcagcaccca tagcaactac ttcatcaggg ttaactgatt tgtttggttc ttttcctgtt
     2221 tctttacgta ctgcttctac aactgcaggg atacgagtag atccaccgac taaaataact
     2281 tcgtcgattt ctgattgtga taaacctgca tcttttagcg cttgacgtac tggtactttt
     2341 gtacgttcta ctaagtcagc tgttaattca tcaaattttg cacgtgtcaa tgtcatttcc
     2401 aaatgcaatg gtccagattc tcctgctgtg ataaatggca agctgatttg tgtgcttgat
     2461 acgccagata aatctttttt cgctttttca gcagcgtctt tcaagcgttg taaagccatt
     2521 ttgtcttgag acaagtcaat gctgttttct tttttgaatt ctgctaccat gtagtcgatg
     2581 attttgttat caaagtcgtc cccacctaga tggttgtcgc cagctgttga caatacatca
     2641 aagacaccgt ctcctagttc aaggatagat acgtcaaaag taccgccacc aaggtcgaat
     2701 actaagattt tttcgtcgcg atctgtttta tctaaaccat aggcaagagc agctgcagtt
     2761 ggttcattga cgatacgttc tacttctaga ccagcgatct taccagcatc ttttgttgct
     2821 tgacgttgtg catcattaaa gtaagctggc acagtgataa ctgctttttc gactttttca
     2881 cctaaatagt cttctgcgaa tcctttgata tattgaagga tcattgctga gatttcttgt
     2941 ggtgtatatg atttaccttc tacatctact ttgtaacctg cttcaccgat atggcgtttg
     3001 attgatgaaa tcgtatttgg gtttgtcaca gcttgacgtt ttgcaacttc ccctacttgg
     3061 atttctccat ttttgaatga tacaacagaa ggtgttgtac ggtttccttc tgggttagcg
     3121 atgatttttg cttcgtttcc ttccaatact gctactgcag agttagtcgt acctaagtca
     3181 ataccgataa ttttactcat aattaacgtc tccttcttta attgaaagtg taagttttat
     3241 atccgcacaa ttattgtgca acaatgacca tggttggtct taacacgcga tcatgtaatt
     3301 tgtagccttc ttgtagtact tctacaatcg tatcggctgg tgtatcttca gtcgctggaa
     3361 cagtttgtac tgcttgatga agattaggat caaaggcttc tcctttagca gggatttttt
     3421 caattccttc ttcttccagt gcgttacgca aactttccaa gaccatttcc acgccttttt
     3481 tcaaacctgc cccttgatca tctgacactt ctgtggctaa tgcacgttcc aggttgtcaa
     3541 ttgatggaag taattttttg gctaaatctt gtgaacggta tttttgtaat tgctcacgct
     3601 cattttttcc tcgatttgcc atatttgcga tttcagcacg agcccgcaag tatttatctt
     3661 ccatttcttc taactcagct tttaatttcg ctgtttcgaa ttcttccgct tcagcttctg
     3721 aaatacccga atcttctgca gcttcgacat cgatttccgg ttctggctga gcatctgtca
     3781 tttctttatc tagttcttct tgattttctt tcacgataca gtcttccttt cattattcag
     3841 tcagctgttt gattgctctt actaagaaag agagaacgat cagctgcaaa tagcttcgtt
     3901 attcacatta cgagtttgac aagtcaagcg aacggtagta atcagcaagt ttagctgcta
     3961 gttctcttcg ataaacatcg actagaccga acatccgaga ataaggcata ctagtaggcc
     4021 ctaacaaggc tattgtacct cttccatgac catctatctc gtaacttgct tgaatcatac
     4081 tcatattctg caataaatca ttgcctaatt cagcaccaat cttgatatga atattgctat
     4141 ccatcggagt gatcatttgt gtaagtccgt ctgagtcttt cataaaagaa aacatcgatt
     4201 tgaactgatc aagatcttga cggggctcat aatccaagag attcatttgt ccactgacat
     4261 aaaccttatc ttcaaaaaca tgaccaagaa cggtgtcaaa taactgagac atcccttctg
     4321 tcgtttggaa atatttatga agaatcatcg gaatctccgt acgaagttta tggtacaccg
     4381 tcatcagagg atctccgact agacggtcgt ttacaatctg taccattttt tctagttcct
     4441 gacttcccat atattcggga agagtgaaca cttggctttc tacattcccc atatctgtta
     4501 cgacgatagc aataatctgt cgattattca agggtacgat acgaaagcca gtcagtcggc
     4561 ggtcttttat ttcaggtccc aatgaaaaag tcgtatagct tgtcagctca gataatatct
     4621 cagcagattg cttaataatc tcattgattt catgaaattc tctgcctaga gactgacgaa
     4681 tcagcagcaa atcatcttct gctacgcgag caggttttaa caaatgatcc acgtaataac
     4741 gatagcctaa agcagatgga atccgcccag aagaagagtg agtcttcaac agcaatcctt
     4801 catcttctaa tgctttcata tcattgcgaa tcgttgctga gctggccttg actccctcag
     4861 acatcaatgt tttggaacca acgggcacgc cactactcgt atagtgttgg atgatcagac
     4921 gtaaaatgtc gttttgtcgc tttgttaaca tcttcctcac ctctcattag cactcatata
     4981 ttctaagtgc taatccactt ataaatatag caagaagccc ttctcctgtc aagagaaaga
     5041 ctccattttt tagcactcta aacacaagag tgctaattct aaataattga tgtaaatcca
     5101 gttatatcaa cgtttcaata gtcctttatt gtatttttga acttttttta tttaaattca
     5161 gataaaaaac aaatattctt taaactattt ttcaaacaaa aagcgctcaa acacattgtt
     5221 ccccaaaaag gtccctcttg gtgtcaaata aacacggtct tcttcattaa caagtaaccc
     5281 ttcttgctct aattccctta gcacatcacc gtacaccgat tcaattgttt tttgatagcg
     5341 atcatagaaa tgttttttag taatccctgt tttttttctt agtcctaaga acatttcttc
     5401 ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt catttgctcg
     5461 taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat ctccaagata
     5521 accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca tgaggttatg
     5581 ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac gtccatgctt
     5641 ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat caggcagttc
     5701 gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga tcaatgagta
     5761 caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca gatatccttc
     5821 tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa aattttctgc
     5881 ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc caattttttt
     5941 caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc catagttttt
     6001 cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag taaactcatt
     6061 accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa gttgtttagc
     6121 agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg ctgtcggatg
     6181 actttctagc attattttca tttcattcag cagcatttcc acatactcat ctactggctg
     6241 cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc aaaatgggat
     6301 atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca ttgaaattct
     6361 ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc gttcaagcac
     6421 caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta aaaatgcttg
     6481 cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg taatcgcaaa
     6541 gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt tcacagattc
     6601 ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg catactaaac
     6661 gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat gattttttct
     6721 ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc tgagtgcccg
     6781 atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt ttaatattgt
     6841 actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt tataatgctg
     6901 gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa gttgactatc
     6961 tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc aattttaatg
     7021 aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag cttgaaaatg
     7081 aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc ttatattact
     7141 agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca atcagaggtc
     7201 acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat acctgaaaaa
     7261 gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca tcatttatat
     7321 caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg tggggcagtt
     7381 gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa tgcgcaattt
     7441 ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca acaagctgtg
     7501 aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac agacttgatt
     7561 cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg tctatacaaa
     7621 cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc attctcttca
     7681 attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat ttgtcacact
     7741 ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa ggttttacgg
     7801 attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt attttatatg
     7861 aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg tacgtctcct
     7921 agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag acaaaccagt
     7981 tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac atcttcaaca
     8041 agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt tgagacaaat
     8101 atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat agatccgtta
     8161 gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat tagaggtaat
     8221 acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc tgcaggaaca
     8281 cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt tatgctcact
     8341 gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg gacaatcact
     8401 agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta tccagcgttc
     8461 attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat tcctacagga
     8521 aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta gactttctaa
     8581 tatgttatac aaaaagaagc tgacagcatt ttgtcagctt ctttcacttt cttttctatt
     8641 tagatttatt ggtctttgat agactccaag gcaggaaaaa attttttaaa taaacgtttg
     8701 caaaacagtc gcttttctat cttctaccgc tacttctgca taggcaccat taatcaatgt
     8761 cagttgtggc ggtacatgac cacaatcaat atcatacaag atcggtagtt ttaattcctt
     8821 tgacagatct tcgtatacat ccttcatttt ataatctaac aaatcctctg ccgcacttct
     8881 accgaacatg atccccgagc tgccatcaaa ccagcctgcc agtttcatct gaacaagtga
     8941 tcttcttaaa tcaactgacg atagctcaca gttttctaga aaccacaaga tcggctttcc
     9001 atcattatac tttcttttaa aagattggac atctccataa ggagtaccga ttaaatgcct
     9061 gatcgtatca atacaaccac ctaataatcg tcccttcatc cttactgcat ctccgctgac
     9121 tgattgccaa tttgttggtt cagtgagatg gaaaacagaa gatgatgaat gctcatgggg
     9181 ccacactttt tgatactttt cagaagattg ctgtagaatc gattctcctt ctcttgtttt
     9241 cagtacactt tcccacattg ctgtcgtttc atccaagtac tctcctctta aatcaataag
     9301 gttagttcca tgtgcagtgg ctatccctgt ttttaatgtg acagcaagta gcaacgtact
     9361 aatatctgaa taacctacta tccattttgg ttgtagagca tcaaaatcga caaaatcgag
     9421 aatctcaatc aataattctc ctccccaagt cggaaagatc atttggatat tttcatcttg
     9481 catcatttga ttgaattcat ctgctcgttt tcttgctgga cctgattttt ccttatcttg
     9541 cgtccaaagc gtctttccag taactacttg gaaagatttt tcttccattc tctttaaagc
     9601 tgattcgagt aacgaatggt gttcctctga tacgcctgag gatggtgcag taaccccaat
     9661 cgttgcattt ctttctaata atggataact tatcatgttg cttcacctcc aagtatgcac
     9721 tattttactt ggttaattgt ttttttcgtt tgccggcatc aaaaatatcg gcatctcaag
     9781 tagctggatg attttgttta agtcaaccgt ttggatgacc atcgtttgat ctggtctacc
     9841 tggtgcaaaa acaagttttt catttgataa gagctcttca tcaataataa tcggaatctg
     9901 tttttcataa ccaaacggag aaacagcgcc ctgcttttgt cccgtttgtt cttccatgcg
     9961 ttctgatgaa acaatagaaa gccttttacc gacgatcttc cctaatgctt tgaaatccgt
    10021 ttgtttcgta gtaaaagtca gatatacgta gttattatct tttttatctt ttaagaataa
    10081 gcttttggtc tctgtacctg taaatccttg ttctttcttt attaccaaag catcttcatt
    10141 tgtataaatg gctcgatgat gatacaactc aaaatatata ttttcgtgta agaatttctt
    10201 tgccaagtca atcattttta tgtctctttt ctattttatt atttgagaca tcttatcata
    10261 taaatatagc ggttacaaca ttacttgtgt tttgttcgtt ttttatacat aactattgtt
    10321 tttctggtaa aataaatcaa accacttaat ggaggcgttt tagaatggaa aaatttcagc
    10381 cgatcatcaa tcaaatcaaa gaaaaagaac atcaacaaaa gatcaaagac ctttttgcta
    10441 agataacaga aacgtatccc cagctagaca caactgtaaa atggaaccaa cctatgttta
    10501 ctgaccatgg tacctttatt attgcattta gttttgctaa aaatcatttt tctatcgcac
    10561 cggaaaaagc agcgattcgt gcccttgaaa aaaatatcca agaagcaggc tacgtatata
    10621 cggataacgt catcaaagtt ccttggaaaa gtgtaatcaa ctgggaattg attgaacaat
    10681 tgatttcatt taatattgaa gataaaaaag gtcatgataa attttggcga taaagcttta
    10741 tacgaataaa ccttttactg gcgaggaaac tattcatcac cttatacagg ttttgttcaa
    10801 tcctatcctt tttatcatcc ctggttaaac acttaattga caaagtatct gctattttta
    10861 taaggagtct ttcttgtgct gaaaaaaatc actgattgaa tggctttgaa ttttctactt
    10921 agtaaaatta ttttttcagc aattttcctg caaaactttt gaataaggaa aaaatacgag
    10981 aaaatatcag tgatctttca taatactgcc cattattttc aggtatattt aaatataata
    11041 tcactttagg agagaacgaa tttctttaga gaaataaaag ttagaatttt ctgacttgat
    11101 ttttacaatg accttaatta aaatcgttct ttctacattt cgtgattaag caaatatcaa
    11161 ctaagtcagt agaaccattc acataggagg gtgaacattg aaagattttt actcagtaaa
    11221 tgaattagcc gaacaattag gtgttactac acgttctatc agaaattatc ttcatgaagg
    11281 aaaactaaaa ggaacaaagg ttggcgggca atggaaattt tctgaacgta atctttttga
    11341 gttcctatac ggagatcaag cagaggaagc agcaaaagaa atgcagcgat tcatgctaaa
    11401 tgcaccaatc acgatgcgtt tcaaccttca atatcgtgat tttacagcaa tcaatcaatt
    11461 ccgagaacaa ttagtccagt accataacga tgtttacgca aataaaaaag accgtctttt
    11521 acaatacgat ctttataaag acaatcatgc ggagatacta atcggtggta acttcaatta
    11581 tgtcgtaaac tttagtcaat ggatcaatga aaaattattg atccagacag atatttccct
    11641 tgtatcttaa acaattaaaa agcgctagaa attccagaga tgttggaaat tctagcgcta
    11701 tttttttata agtacttact gtttggttca aacaaatatt tttttaataa atgaaatttt
    11761 accatcttta tcctcaaata aagaccgtga ccatgcttcc tactacgatc agcaataatc
    11821 cgataatcgt taattttttt tcttttggtg tcttattttc atgcaggatg tacataccgc
    11881 ctaatgtaga gataactacg tttagctgtg taatgatata tgcagtagca accccatttt
    11941 catttgctga aaaaatataa gccaatgctg acacactaaa gatggctcca acgatcgttg
    12001 ctttccaact cttgtcttca gaaaatgcgg ttggtttacg gaaaatcaca taaatcactg
    12061 cccctaaaaa gacaccgatc atttgcggaa agaaaatgga aagtccgctt gcatcaacta
    12121 gtttaggcaa tgcactgtat acccaataac caatacttgt tgatgctaat agactaattc
    12181 cttttgtgat accgcctttt tttccaggat catcactgat tgccgttaaa gcagaaccaa
    12241 atacaagaag aatgattgct gctgctccaa tgattttccc catacttcca gaccattctc
    12301 caaaagcgaa gacaccaatc aacgaggttc cgactaactg taatgcagtc gaaattggca
    12361 tcgttttaga aacaccaatc aagttgaaag atgtatactg tcctgtttgt ccaataaccc
    12421 aaaatgcacc agaaagtaaa ctaatcaaaa atgtagtcac tgaaatacca gctggagaag
    12481 cgaataattg aacgagtaag ccgacaagca ccgctccaat accagttcct aatatctcat
    12541 ttgttggtct gccaccaatc tttttcaaaa tcagtggctg gatcccccag ccaactgccg
    12601 ggatcaatgc aatcaataaa cccattttta ttcaccctta tccttttcca ccttggaaag
    12661 aaggatataa agtcatccca ccatctgcaa atagtgtaat acctgtaaca taactggatt
    12721 cgtctgaagc caaccatgca gctgtcgccg ctatttcttt tggttcacct acgcgtccta
    12781 gtgggatcat actttccaag agttcttttt gttcaggatc atcaaatttt gccgcattaa
    12841 taggtgtacg gatagcacca gggcctatac tattgattct gattcccttc ggtgcatact
    12901 ccattgctac cgtttcggta aataatttta ccccgccttt tgaagcagcg taatgagcaa
    12961 acgttggcca tgggatctgt tcatgaacag aagacatatt gatgatattt ccttttttat
    13021 tattctctgt aaagtaattt aaagccgctt tcgttccgat aaaaacgcca gtcaaattga
    13081 cttttaacac tctttcccaa tcttcaaggc taagttcatg ggttggatgc tgattttcca
    13141 tcccagcatt attaacccaa acgtccagtg tcccaaaatt ttctaaagcc gtatcaagta
    13201 aacgctgtat tccttcttca gaactgacat ctgcttgtac agagactgct tttccgcctg
    13261 cctcttcgat tgctttgact gctttttctg ctccttcttt atccgagtta tagttgacaa
    13321 ctacattcat tttttctttc ccgaatcgtt gagcaatcgc tgttccaatc ccctttgagc
    13381 caccagttac tactgcgact ttgttttcta attccttgta catagtaccc actccttttt
    13441 catacctaaa cctgttatta cggataactt ttatttccgc cttaaagtga gtgtacacca
    13501 agatatatag tctaaagcaa atgatacgct ctagcaatcg ttttcatgaa ttgctagtgt
    13561 ccttttattt ctataaagaa agaggttgtg acagaagtgg tcagcttcaa gaaatttcgg
    13621 cttatttccg aagaagctac ttctgaaaaa ccatttattt ggaaaaaaag gctgtgacaa
    13681 gacttttgtc acagcccctt tcctgggctt atttatcacc cattttattg taattgctag
    13741 ttatacataa acaagagatt ttatgcataa tgttttattg ctgagaaaaa tattcagctg
    13801 ttgcttgttc atcctccttg agttgttcaa tcaattcctc tacactagaa aatttcactt
    13861 gatctcttaa aaattgattc cagcgtaccg cgacatgttc accatagata tcttgattaa
    13921 agtccaaaat atacaactct acagtcagct cccgtccttc accgaatgta tcattatgtc
    13981 caattgaacc catggctgga taccatgttt ccccaatttt tatttccgac acataaacac
    14041 cttctttagg caaatgaacc gtgctcttta ctttgacatt tgctgtaggg aatcctagta
    14101 gtctgcctcg tgcatcgcca tggacaacta ccccttctac ctcatatgtg caacctaata
    14161 gttttgctgc ttcttttact tgtccgctgt ctagtaatcc acgaatacgg gtagagctga
    14221 ttttttcacc ttgttcttct tctttagaaa ctgtgacgat tttgaatctt cctttggcat
    14281 attcaggaaa atgagcgaca tctgcaatgt cttttggtcc atacgtataa tcaaaaccgg
    14341 aaacagcata ttttgcatgt aaccctacga tgtattgatc aacaaattcc tgcggtgcta
    14401 aatgagcaaa agaagaagta aaatcaactt catataaata atctataccc aaagcggcca
    14461 ttttttcttc tttttgctta aggctagtca aatatttcac gctgctagga tcgatctttt
    14521 taaagacaat agatggatgc tgattgaaag tcatcaatgc taatttcaat ccttcttttt
    14581 gtgcaatttc catacctgtc tttatgactt tctggtgtcc gatatgcacg ccgtcgaaaa
    14641 atcccagcac taaaacgaca tcgtcttctg gaatttgttc tgattggtaa ggatgtctga
    14701 tcttgatgat attcattcgt tattcctcat ttctcaatac tttactaggt tttaatttgt
    14761 ttttttcttt cgggtttggc tgataaatac tgacaacttt tcctttataa aacaaagcaa
    14821 tttcttctga cggcatttct gggagcgcaa atacagtata atcaagtctc ataccgtttt
    14881 ttactttctg ccatacttca tcagtaatat ctactcgttt gaaccgttcc actccttttt
    14941 ctattggcag caaacaatca tcgattgtcc catttttcac agcttgagct acttcttcta
    15001 atgtcagagc atccttctgg gacataccgg cacttgctgt tcgggtcaga tcagacatgt
    15061 gcgcagggta accaagtttc ttgcctgtat ctacagctaa tgtgcggaca taggttcctt
    15121 tcccgcattc tacttcgaag cgccaagaaa tggttcctgt cacttcgtct cttgtcaatt
    15181 cacttgtccg gataaaacgg taaatctgag cctttctaac aggacgctcc actgtttcat
    15241 tattgcgggc atattcatat aaccgcttac cgttgacttt taccgctgag tacattggtg
    15301 gtatctgggt gatctcacca ataaatgaag ccatcacttc atctattttt ttttcagata
    15361 aagacatatc tactggtttt gtttctaccc gttctccgga tcgatcttcc gtcgttgtac
    15421 tgaaacctag agttatctcg cctacataga ttttcccaga atctgtcaaa tattcgataa
    15481 cttttgtggc ttttccaata caaatgggca acacaccgtc tacgtctggg tcaagcgtac
    15541 ccccatgtcc gatttttttc gtatgtaaga tttttcttaa tttgaatacg caatcatgac
    15601 ttgtcattcc gcgttctttc cataaaggta ataacccgtc catgtctttt ctccatttca
    15661 tttttgattt aactgaatta ttataacaca ttgcatcaat tactgacgaa tttgacatca
    15721 taatgaacgg tttttatttt tcggcaataa aaacgtaaca aaattcatgc tcacgtaaat
    15781 agaaatcatg ttatgctttt ttaaaggagg gaatagaatg gaagattttt tctttgcacc
    15841 atttgattgg atttatgggt ggcttacagg taaatagttt ggttttcact tcccaaaaac
    15901 gaaaaaaaag actggccatt cttccagtct tttttccgtt ttttttattt attttttcag
    15961 tttcttaatc aatgtaaaaa attctatttt gcgggatcga ttttatcatt ttattggtca
    16021 agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc acgaatatag
    16081 ttttgatgtt cttgtaattt ctgatccatt tttttcgcaa aatcttctgc ttgacttcct
    16141 ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt tactacacgc
    16201 atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat taaatgatgg
    16261 ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa agcaaataag
    16321 atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc acgcggatct
    16381 tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat ttttagtttt
    16441 gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga ttccgttcct
    16501 gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca atcaatgatt
    16561 ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg agcacgcgga
    16621 tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt atccatgact
    16681 gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc cttcttttct
    16741 gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc ttgctggaag
    16801 actgttgagc ttgcaatgag atttagcgaa ggataatcat ttcgccaaga aaggtcatgt
    16861 gatttccgca tccatttgaa gtgctgagtc aacatagagt caaccactcg caagaatgat
    16921 tcgtaacttg caaaaaacca tgccgtccag tcagaacgta cccttctaag aacccttcat
    16981 cttggtgttc cgataattga ctgtcgatga cgcgaccaga tggcgaaagc cattcatcat
    17041 tttctggttc gattttttcc atccattggc gatttgtcgt tttgaaaact tgattcaatc
    17101 ggtttgattt tgtttcatct ggtccaaata tacggaaatt ttctggattt ttctcaatga
    17161 tctcgcgaac gaatttacca agttcggtca tatcttctgc tttaacggag cctggttttt
    17221 caaattgcaa agtatatttt ttccaatctg gcataataag cggtcttgga tcgattccac
    17281 cattggtgat aggatttttt gccattcttt ggtcaccagt aggcgcgatt tctctgattt
    17341 cttctaaaat acgtccttgt gcatcaaata atttttctgg ttcataggat ttcagccaac
    17401 ggagtaaagc atctgcatga tccatatgtt cttgatctac aggaatcggt acttggtgtg
    17461 ctcggaaggt tccttcaatt ggttccccgt cccattcttt tggaccggtc catccttttg
    17521 gtgtgcgtac gatcaatact ggccattttg gcatcacgac atctgttgct gtcttcaggc
    17581 gagcttcttt ttggatggat ttaattttct cgattgcttg atccattttt tcagccataa
    17641 caggatttaa tttttctgaa tcatttcctt caataaagaa aggttcccag ccaagtccgt
    17701 taaagtaatt tgctaattct ccatctgatt tacgtgccaa gatcgttggg ttggctattt
    17761 ttgcaccatt taaatgaagg atcggtaaaa cagctccatc tatcactgga ttgataaaga
    17821 catttgagaa ccagcttcca gctaaaggac cggtttctgc ttcaccatca ccaatcacga
    17881 cagcagagat tacttcagga ttgtctaata cagctccgac tgcatgtgaa agagagtatc
    17941 caagctctcc gccttcatgg atagaacctg gtgtttctgg tgctgcatga gaagcaatgc
    18001 ctccaggaaa tgaaaatcgt ttgaataatt tttgcattcc tgtctcatct tccgttactt
    18061 ctggatagat ctctgtgtaa cttccatcta aataagcatt cgataccatg acttgtccgc
    18121 cgtgtcctgg cccttcaata taaaacatgt tcaaatcata tttattgatg actcggttca
    18181 aatggacata aataaaattt tggccaggaa tcgttcccca atgaccgatt ggatgttttt
    18241 tgacatcttc gggtttcagt gtacgtctca gtaaggggtt atctttaaga taaagttgcc
    18301 caacagaaag ataattagca gcacgccacc aagccgtcat tttatcaaag tattctttac
    18361 tagaataatc catacataac actccttttc attattactc ttattctagc gcagatactc
    18421 ccaattgaaa cctaataagc acttctgttc atttgtttgg attttatatc tagccaaatg
    18481 aacaggtata aaaaaccacg aaatttttcc tttcgctgac gttttttaca ttgatccaaa
    18541 aaacttgtcg ttatcacaat ggctgttccg cagttcattt cgctcctcat catgcataat
    18601 ttgctgaatg acgctgggcc agttaacaca ttgctggaaa actggggctg gatcgagcaa
    18661 ttcctttcct gattgatccg ttttatgtaa aagtctctgt tatctttgtc aatatgtgaa
    18721 ttggtatccc agctactatg ctaatttcta cagggattat ccaaaatttg gatcgtgacc
    18781 aaatcgaagc ggcgtaatcg gaaatatcaa taacttcaac gtgatttttc ttttaactgt
    18841 caacggaccg acaaactcaa acttttatgg tgcaggttcc actgatcttc tagttacttg
    18901 gctatacctg actgtcacta aaatagatta caatctagct tccgttatcg gtatcttgat
    18961 ctttatctat atgcaaaaat ttatatacta ctagaaaaaa gaaagtcgaa ttttttactt
    19021 ttctttttct cttttttggt tagctgacac gccgcaagac taaagtcatg gaactcttag
    19081 gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca ggatttgcgg
    19141 gagtttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg ttgccataga
    19201 caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt agattgtgcc
    19261 ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct gttttcttat
    19321 ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag tcccccgtaa
    19381 ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt ttgcgcaaaa
    19441 tatcattgac ttcttttaag atttcttgac cgactcggcg gtcacggtag ttagccataa
    19501 tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga taaatccttc
    19561 aattacgtca tctactttga tgtcattgaa tttttcaatc attgcaccac attcataacc
    19621 cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt ggccttcata
    19681 gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat atccttctgt
    19741 tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac gaacgatcat
    19801 ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg cagtttcgat
    19861 ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt cagtatcagc
    19921 ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag cgttgctggc
    19981 tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga cgatttttac
    20041 acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg ctgaaccttg
    20101 tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca ggctttcaaa
    20161 taagttatcc aaagtcacac ggcttgtggc agcacggttt tctaataatg cacgtttcgc
    20221 gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga aacggtcacc
    20281 tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc ctgcttcttt
    20341 atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat taccgacaac
    20401 gattgggtca ccaacatgaa gtgttccttc ttggacaagt aaggttgaca caggaccttt
    20461 acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg gatctgcttt
    20521 taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga tgttttggcc
    20581 gaatttcgct gagatctcaa caaagatcgt ttctccaccc catgcttcag gaattaactc
    20641 atactcactt aattcttgca taacatgttg agggtttgca cctggtttat caattttatt
    20701 tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt caactgtttg
    20761 tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga tacttgctcc
    20821 acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag tgattggttt
    20881 accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg cttcgccgct
    20941 tgttacacgt gaatggcgta atgtatccaa taaagttgtt ttcccatggt ctacgtgtcc
    21001 cataatcgtt acgactggcg gacgtgagac taatttatct ggatcgactt cttctgcctc
    21061 gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt ccatgccata
    21121 gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat tgaccatgac
    21181 accaagcata aataattttt taatgatttc tgctggctca cgatgaattt ttttcgcaat
    21241 atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac ggaatttgcg
    21301 tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt ttttattata
    21361 acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt tgttgttatt
    21421 gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc tgctggtatt
    21481 tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac cgctcccttg
    21541 accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg ttgtttcttt
    21601 tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt gtgcagcttg
    21661 ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcat tgctagtaat
    21721 cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca cgacgtcttt
    21781 actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca tgcaatcacc
    21841 ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca ttattccgaa
    21901 taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata gctcaaagca
    21961 aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct cgcttgcatc
    22021 ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg tcagttcttc
    22081 acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg ctttttgccg
    22141 attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt gacatagtca
    22201 agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc gagaattttt
    22261 ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc tcgtccaggc
    22321 atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat tcggaccatc
    22381 tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat tttcctttgt
    22441 ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac cgtttcattg
    22501 ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat tgcttcatca
    22561 tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt catgtcagat
    22621 tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc gttttgtccg
    22681 cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca ggcctttgga
    22741 tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt tgcaatgaat
    22801 acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa ttcattgacg
    22861 attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc gatatttgga
    22921 tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat gctgacaatt
    22981 tcaacgattc catcatagac ttctggtact tcttgctcaa ataagcggcg caataggtct
    23041 ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac tcgggaaaca
    23101 taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc ttgtttagaa
    23161 agaactgcct caattttacc aagattgaca taaatataac gtttatcctg gcgttcgacg
    23221 attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat cgttctttct
    23281 gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat ccgcccaaaa
    23341 tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg attgattaaa
    23401 agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt tacttctttt
    23461 accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac gttttgtgct
    23521 tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc gatcacgatt
    23581 tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat ttctttactc
    23641 attttttctg attcctctcc gtgattaaaa ttggatagct aagcgagctt ttgcaatatt
    23701 ttttcggtca aaagtcaatt ctttttctcg tgtttttatg cgtattttca atgtaagctg
    23761 ttcagcatca aatgattgaa gaaatccttc atattgtttt tcgccatcta ctggttggta
    23821 taaggagaca tggacataat caccaagtgc cttttcatag tcgctttctt ttttcaatgg
    23881 ccgctctgct cccggggagg atacctccag gaaataagct tgaggaatcg gatctggatc
    23941 tgctgcatca agtttttcac tcagtttctc actgacaaag gcgcattctt cgatatcgat
    24001 accgccttct ttgtcaataa atactcgtaa gaaccagctt tttccttctt ttacaaattc
    24061 gacttcgaca agttcaaaat tttgttcatc taaaatcggt gtcaccattt ctgtgactgt
    24121 ttcaacgacg ctactcaaaa gtttcgcctc cttaaaaaat taagtgcgta attccattaa
    24181 aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata agaactatac
    24241 catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca tatcgaataa
    24301 cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca tatattcgat
    24361 caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa atggaccgtc
    24421 ttttcttgct tcaacgattt gttttgccac gttggttccc aaacttggga cagctctaaa
    24481 tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt tatacaaatc
    24541 aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca gcactgtcag
    24601 ttgattcttt tcttttacgg aagcatctaa tcctttgtcc gtgatttctt tcattgcttg
    24661 tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg cacgtacaga
    24721 aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga cacgaagagc
    24781 catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt ttgaacaaga
    24841 atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg tttctcgcaa
    24901 ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt taaatgccat
    24961 cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac agccgattac
    25021 ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta accatacgtc
    25081 tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag gatgcgtttc
    25141 ttctagcatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg tccctgtttt
    25201 ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata tccgcatcac
    25261 ttccgggtca tccgtaggga tcgtttgtgg atcgatccct gataaatctt gaagcatccg
    25321 aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat cgtgaatcga
    25381 gtgaaaatca aaatgagtcg ttttccattc cgaattttga tcgtctgctg gatattggat
    25441 cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc ctggatgctg
    25501 gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt ctgcacttcg
    25561 aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg ttttgtcggc
    25621 gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata ctttcgtata
    25681 gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg gcactttgtc
    25741 tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt ttaagcgtgc
    25801 accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg taccatcttc
    25861 aaaaaactcg gaatattggc actctggaca ataataatga ggggctaacg gattaacttc
    25921 agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg aacctactag
    25981 atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa taaccgagaa
    26041 gccattacca ttaatagaat tcaactcttt ttttaatcgt ttttgaatga tctcaggtaa
    26101 cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt catcttctga
    26161 acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt cgcagatatc
    26221 tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc ctaaaaagtg
    26281 aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac tatgacgatt
    26341 caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg catcttcttc
    26401 gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt ttccgatttc
    26461 taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt cttgctcgat
    26521 caaaggcgca tagaccgctt ttggcataac ttcaatataa tcgtagaatt ttgctcggtt
    26581 tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct tatcacaagc
    26641 tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta ttcgtgggac
    26701 gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt tcagtcctgc
    26761 ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag aatctccttc
    26821 tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca ttgcctcttt
    26881 cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc ggtgatgctg
    26941 ttccaagctg acaccaaatt ttttagataa cacacctaat ccgaaacgtt tgaattgagg
    27001 atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt caggaatacc
    27061 ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt gggctaccaa
    27121 aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg actttgatcc
    27181 tcgaaccatt ccatccgtga taccagtcag atctaccgtt gttcgtgaca aaggatgtcc
    27241 tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt tgactgcggc
    27301 tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt caaatacgac
    27361 atatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga caccatcatc
    27421 tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc cagcactatg
    27481 tgcttccgga aaggcttgcg caccaccgtg gtctgtaatg gcgatagctc ggtgtcccca
    27541 tttccctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg tactcatatt
    27601 gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt tgcgaggtgt
    27661 atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa acgtatcttc
    27721 ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa atacttgttc
    27781 atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat ctgtgatttt
    27841 caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga atacgtaccc
    27901 ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg ttggttcatc
    27961 ggctggaata tttcgtccta gctggatgtg gatgggtcct tcaagagctg gcgattgctc
    28021 ttttctttcc tttttctttt gttcgtggac aactagactt tcagctgctt gcttcataaa
    28081 ggcttctgcc tgttcttgtt tgcgttcttc aaaaagtttc aatactcgtt ccgcttgctg
    28141 ctcatccact tctggttcga tacgaaattt tgggaatccg taactgacga acaactcttc
    28201 tactaatggc aagtattgtt gcttcaaata agaaatagct cctgtactgt cgattggcaa
    28261 gatgacttta cgatctttta tcacaggtac ctgcgttttc aatacttgct gtgctagtgg
    28321 tgtatcgcat tgctgatttt ccaaagcttg ggcccagtaa tcttgcaaga gctgttcatc
    28381 gaatgtctgg tcatctgcta agatattcag tttgacttgt gcaatatctt taaaagcgat
    28441 agtcagatgc tgagtcaatg accgatatag cataataggc aaaattttgg aaaaatgcag
    28501 cgtaaattcc cataaacgcg atttacgatg gaccactaca ctatccattt caccagcagt
    28561 aagcagtgga tgattttttt ctgcttcctc tagttggatt tgttccaaca gtttttcaaa
    28621 taaatctcgt ttttctgaca aagatatacg ccctttctga tgagaaacaa tgcaattgat
    28681 cgcatcttat cctcttcttt ttcagtacct tttagtatac tcgttttttc tgagttttac
    28741 attaaaaaat tggaactgcg attcaaatcg cagttccaat tttaaaagcg aatcatcaga
    28801 ttgtcaaaaa cagctgattc agctattttt attttctgtg ttttaacgct tattattcgc
    28861 cttggttcaa gaggattggt aaagtattca ccaattcttc tttacgtact tcaaccattt
    28921 cgccagtttt cttgattttc acttctacga ttccatccac tgctttttta ccgacagtga
    28981 ttctgattgg acagccaatc aaatcagaat ccgcgaattt cactcctgcg cgttcatttc
    29041 tgtcatcaac cagtacttca tagccagctt ttgtcatcgc ttcttctagt tcattcgtca
    29101 ggtttgtttg gtattcgtct tttagattca tttggacgat atgcaaatca aatggcgcaa
    29161 ttccttttgg ccagttgatt ccttgttcat ctgaatgttg ttctacgatt gctgataata
    29221 aacgacttac accgatacca taacagccca taatcacaaa tttttctcgt ccattttcgt
    29281 ctagtacagt tgctcccata gattcactgt aacgagtgcc gattttgaag atatgtccga
    29341 tttcgattcc tcgagtaaag gcaagaacac cattgttgtc tggtgaagga tctccttctt
    29401 gtacaaaacg aagatcttca taagaaagta cagtgaaatc gcgttctgga ttcacatttt
    29461 tatagtggaa tcctgtttcg ttcgctccag taatggcatt cgctaaatct tggacataac
    29521 ggtctgcata tacttttacg tcatctgaaa caccgactgg tccgacagaa ccaaactcag
    29581 cacctagata tttttgtgct tcttcttctg ttgcttcttc taaaaagtca gcagataaaa
    29641 agtttttcag ttttacgtca ttgacttcat ggtctccgcg taccaaaaca aggacaggtt
    29701 gttcatccgc aatgaacaat acagacttga tgattttttg aggttcaaca gaaaagaaat
    29761 ctgctacttc gtcaattgat tttacgttag gtgtagctac tttctccaat tcaagctgtg
    29821 tttcatgcga tttttttgat acatagtagc ttgtagccat ctccaagttt gcagcataat
    29881 cactttcagt cgaataacag atcgtatcct ccccgatttc tgagatcgcc atgaattctt
    29941 ttgagtcttt cccgcccatt gcgccaccgt ctccgataat cgagcgaaat tctaagccac
    30001 aacgcttgaa tacttcggtg taggcttttt cgtaatcttt atatgtctcg tccaaacttt
    30061 cttccgttgc atggaaagaa tacgcatctt tcatgataaa ctctcttccg cgaagcaaac
    30121 cgaagcgagg tcgtttttcg tcacgatatt taggttggat ttgataaaga tttaaaggta
    30181 atttcttata tgaattgatt tcattacgca tcaaatcagt aaaagtttct tcgtgagttg
    30241 gtccaaggat catttttcga tcattgcggt ctttgaaacg atacaagttt ggtccatatg
    30301 tttcgtaacg cccggattct tcccaaagtt ctgctggcaa gatggcaggc atcagcattt
    30361 ctaccgctcc gattttttca aattcttccc gcataatgcg tttcagtttt tccaatacac
    30421 gatttgctaa tggcagatat gaataaatac ccgctgatac ttggcggata tatccagctc
    30481 ttagcaagat ttgatggctt aatacttctg catcatttgg aacttcgcgt aatgttggaa
    30541 tcaacatttt tgattgtctc attctatata ctcctttatt tcctcaaaat agttatcgtt
    30601 tgttattata ccctatttgt caactttttc tagaaaaaga atcgttgaat atcgttccat
    30661 gtaactagca ccatcaacaa cattaaaaat ccaaagccag ctaatgtcaa tattccttct
    30721 ttttcttgac ttaatggttt cccgcgaatc ccttcaaaaa tattcaagac taatttcccg
    30781 ccatctaagg caggaatcgg aagcagattg acgattccga gattcattga aagaagcgcc
    30841 atcaaactaa taacagttgt tacaccttga tttgctgctt ctgaagacaa ttggtacatc
    30901 atgacaggtc cgcctaattt atctaaactg aaacctgtaa atagtgatcc taaggctttg
    30961 aaaatttcca atgaaccgct gaaagcttga cgcgtccctc caatgatctt atccataaaa
    31021 cctgtattca ttggggcttt tataccaagt tggccaactt tttcgccatt tgactctaca
    31081 gatttcggtg tcacgtcgac agattgcacc tgaccttctc gttcgatctt aaagtctaat
    31141 gttttatccg gatttttcgt aatgactgtt gtcagatcat tccatgaatg gatttctttt
    31201 ccgtccacac tgactacctc atcattttct tttaagcctg cttctgccgc agctccatta
    31261 ggcatgatct ctcctacgcg attagtgttc gtcacttgaa caccaccttg cataaatgcc
    31321 agaatgatga ataacaggat tgccaaaata aagttattca tgggtccagc aaagttggtc
    31381 agcatacgct gccacaattt agccgattgg aactgtacgt cttttggcgc aattctgatt
    31441 tctgttccgt cgttttcaat aatcgtcgca tcgtgatcaa cagggtaggt tactacttct
    31501 gtttcgtctc cattcacata accagtgata gtcagctcat cttctaaatc ataacggctc
    31561 aattccatcg ggatagcatt tgtcaattgg attttcttac ttaaatttat cttctctacg
    31621 atgccgtcag aatttagtaa aagagaaaga ggcattcctg gtgccatttc tgtttcatca
    31681 tccccattgc ctgccattcg aacataaccg ccaataggta ataggcgaag cgtatatgtt
    31741 gtcccgtctt ttgcttgatg cccataaatt ttcggaccca taccgatagc aaattcgcga
    31801 acgaggattc ctgatcgttt cgcaaagaag aaatgaccaa actcatgaac aatcactaat
    31861 ataccaaaaa cgatgataaa tgtcagaatc gttttcatag cttttttctc ctttacttgt
    31921 tttttgtgtt ttttataaaa caacgataaa acactataat tttaccatag tataccaaag
    31981 acaccaatag tatcaatata atgaaaaata aaaacctgcg tcttttatga aattcacaaa
    32041 gaaacgcagg tatacttact aaataatgcc aaataaatgc atgataggaa atacaaacag
    32101 caaactgtcg aaacggtcta agattccacc atggcctggt aagatatttc ccgagtcttt
    32161 cacatcataa tgacgtttga ttgcagattc gaccaaatca ccaaattgtc cgactgctga
    32221 aaaaataatc gtcatcagaa gcatgacaaa cataccatga ttaaatagtt cttttgctgg
    32281 gtatagcaat aaataaagaa atgcgactac aacagcacta ccaataccac cgagcgcacc
    32341 ttcaatcgtt ttgttgggcg aaatatcagg acttaactta tgctgaccat aacgtcgacc
    32401 aaccatatat gctccgatat cagttgccca aacaataaat agaccaaata tcaaaacagc
    32461 caagctttct gttcgtgcat tgacaaaatt ctggaaacct attcccacat acaagctgac
    32521 aagcacagga aaacctgctt catcaatcgt atacgtattt ttagaaataa ctgatgttcc
    32581 taaaagaatc ataactataa gataaaataa cataaatgta cttacattct ctggaagaaa
    32641 gaaaaaccat ctttcttttg gcaaaacaag taacactgca ccaacggcgg ataaaactcc
    32701 ttcaaaactt aagagtgtca atcctttcat ccgaaacagc tcgtaaactc ctattactgc
    32761 taagattgct gctgtaagct caaccgctat gcctccgatc catataatcg gaataaacaa
    32821 gactagcgcc acggctgctg tgaccacacg ttgtttcatg actgatcccc tttctcatct
    32881 tctgtttttt ttacaccgcc gaatcgacgg tctcgatttt gataagaggc gattgcttct
    32941 tcaagatgcg caccatcaaa atcaggccaa agcgcttttg taaaatacag ctcactatat
    33001 gcgatctgcc ataacagaaa attactaatt cgctcttctc cacttgtacg gatcatcagc
    33061 tcaggatctt gtaactcttt aggcaagaaa cctgtcatta aatgcttagc aatcaattca
    33121 tcatcgattt cttctgcgtg tatttctcct tttgctactt cttcggcaat ttcttggaca
    33181 gcagtcacga tctctgctct gcttccataa ttcaaagcaa aattcaatac cattccagta
    33241 ttgttttttg tttgctcaat tgcacgtctt acagcatctt gcgtatgttc tggcaatacg
    33301 ttttcataac ccatgacatg aactttgaca ttttctttaa tcaattctgg tacgaacgta
    33361 tcaaagaagt cgacaggtag ttgcatgaga aaacttactt catcttttgg tcgcttccag
    33421 ttttcagtcg aaaaggcata aagcgtcaac actttcactc caagccttga tgcttttttt
    33481 gtgactttct ttactgtttc catgccttct ttatggccag caactcgtgg caaacgacgg
    33541 ttttgcgccc aacgaccatt gccgtccatg atgatggcaa tgtgctgagg aattggtcca
    33601 tcggtattaa attgaaattc gcttttttct tgaatatatt tgtttttttg cggaaaaaaa
    33661 cgtaacattc tttttcctcc tatacttaaa agattcccac tatcttaagt attatagcaa
    33721 tatctaactg tttttcctat gaaatcattg tttttttatt taaaaactga ataatttttt
    33781 ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg gaatgatccg
    33841 cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta tcagcagcga
    33901 tcttatctac ttctttgatg ctgttatctg tcaatgtttg tacgtctttt tctaatccgc
    33961 gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca tcgatcgcat
    34021 cacgacggat gttacgaatc gcgatttttg cattttctgc ttctttttta acatctttag
    34081 ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg acatttccat
    34141 cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca tcttgaagaa
    34201 tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg attgatgcca
    34261 tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc aaaaggctag
    34321 cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt gctgcttttt
    34381 tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc cccttacagt
    34441 tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat ttaaattgaa
    34501 gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat ccatgacttg
    34561 taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg catttgcatc
    34621 tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca ggataacatc
    34681 tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc
    34741 ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg ctttacggcg
    34801 aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc
    34861 cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta acatacccat
    34921 gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc gccagatatt
    34981 tccgccgccc acaacgattg ccatttcaac gcccagctcg tgaacttctt tgatttcttg
    35041 gatgatttct ttgattactg gtggtttgat tccaaatcct gcgtccccag ctaaagcttc
    35101 tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca ttcctccaat
    35161 attttctatt gccattttac catacttaga tgatttcgac actctaagac tggtttttct
    35221 tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa
    35281 ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt tttcgatacc
    35341 ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg caacgtattt
    35401 ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga tttctgcttt
    35461 gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt
    35521 caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg gaatttgtga
    35581 ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc ttgcaactgt
    35641 ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca tatgcaagta
    35701 tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga agctgatttt
    35761 ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt tatctgtttt
    35821 gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc
    35881 ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg agtttacttc
    35941 aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag ctgcaatgcg
    36001 gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa ctgctttttc
    36061 catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta cgccagtcat
    36121 ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact cctcctgtgt
    36181 gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga
    36241 aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac aacatctacg
    36301 atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac tacttgatct
    36361 tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa tttaacggca
    36421 cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc gcagtttgta
    36481 tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc aatacgttct
    36541 ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc gataccgcct
    36601 aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt tttaggtaga
    36661 acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct gatacgtttt
    36721 tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac gaagtattgt
    36781 cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt acctacgaat
    36841 aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc atctactaat
    36901 ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa gatatatttc
    36961 ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc ttctagtaat
    37021 tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt tttcctcttc
    37081 ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca ataagaatgt
    37141 ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc cgtatcgcaa
    37201 gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa aaagaattca
    37261 agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa ataattttag
    37321 gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata catgtgaaat
    37381 ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat taaacaagtc
    37441 attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg gcattatggc
    37501 gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat cgtgaaggca
    37561 cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact
    37621 aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt gaaagtcgaa
    37681 cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt atttgcatct
    37741 gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt cgatacgatc
    37801 atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct tcaccatcca
    37861 actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc gtgttttttt
    37921 attttcaata ttttccttat tgaataaacc tttacactct ttacaaaaat acatcatttt
    37981 ttattcaaat ttttcaacac cttgattgaa tcacaataat catcatttat attggatgtt
    38041 aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg
    38101 tataaattat taacttctgg accattgacg acttctgaaa cagtgaaaaa tgctatgctg
    38161 atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat aagaaaaaaa
    38221 ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat gcaaggtagc
    38281 agctccttcg gggtagaatc ggtattaagt tctgtgatca ggccagaaga tactgtactg
    38341 atttgttcaa acggcgcata tagacaacga atgatcaaca tgtgcgaatg tctgaaactg
    38401 aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt gattacacag
    38461 ctgattgaag agaatccgtc tatctccgca ctagcaatgg tccatagcga gactacttcc
    38521 ggtattttga ataatttgga agagattgct gctgtcacac aagaaagaga tcttttgttt
    38581 atagtggacg cgatgtcaag ctttggtggg ataccgattt gtgtagccga actagggatt
    38641 gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt ttcttttgta
    38701 cttgctaata aagcggaact ggaaaaacga aaaaatgtta gccgaagttt gtcacttgat
    38761 ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt cacttcccct
    38821 acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc
    38881 gaaactcgat attcacgtta tctcgccaat aatcatgtgc tacgacaaaa aatgacagag
    38941 atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc ttttctttac
    39001 cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca aaatggattt
    39061 gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa tattggcgag
    39121 atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa ggagaacgtt
    39181 aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg gctgtatggc
    39241 tcctgtccac gcttttagaa atgcttttct agaaaaagga atccaactga ctgacaaaga
    39301 aatccgctaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc tttccctccc
    39361 ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa aagaggtgga
    39421 ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc attctacctt
    39481 gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca atattggttc
    39541 cacgaatgga tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa aaaaagaaag
    39601 gtattcacca gacttcatgg caacttttga agacgtcgaa gggtatggtc gaccgtttcc
    39661 ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg aagtaatcaa
    39721 agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt tgacagtagg
    39781 tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa aattagagat
    39841 ggggaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc cggcgctgat
    39901 atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga catgaatcca
    39961 gaatgacttt tatacaaaat aagaggttgt gaaaaagctg tcctgtatca agtaataaaa
    40021 acagccaagc aaaaatagct cctcatattt ttcgtttggt taggcttatt accgcagatg
    40081 caagcttttg aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg acttttgtca
    40141 cagtcccctt atttgcaaac aaacagtgtt taagagacaa cctctccact aaacggtgtt
    40201 cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag ctattttctc
    40261 ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt tcttacatct
    40321 tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca gtcactgcat
    40381 aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc gtatgagcat
    40441 agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact gacggttcgt
    40501 atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta accacttccc
    40561 agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat cctgcttttt
    40621 ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt tctccttcaa
    40681 atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt
    40741 ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt tccaaatcag
    40801 ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat acttcttcta
    40861 ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg atcaaaccaa
    40921 atggaatatg gtgcatgtcg tcagaccagt caaagcccat ttccttcaag acagctttca
    40981 actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt ttgaagtcgt
    41041 aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt
    41101 ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg atctctgcac
    41161 ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca tccatcttgt
    41221 cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga tcatagattt
    41281 tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata gcctctttat
    41341 cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct ggttctgttt
    41401 cagctacttc atggaattgg acgtataaac gaagcaattc attgattggt tctgctttaa
    41461 cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac tgtgttcccc
    41521 agtcacctaa atggttgatg cggattggct gataaccgat tttttccata atgaaaccga
    41581 tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg gcaatattag
    41641 gagaagacat gtcgattggt actgttcctt gattcccgat gttgctatcc ccataatgtt
    41701 ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa
    41761 agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg attttttctg
    41821 ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg
    41881 gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgc tcgacttgtt
    41941 ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga tctttattat
    42001 tcatctttat tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat ctctagcgac
    42061 ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact ccaattgtcc
    42121 taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa acagaattct
    42181 ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa ttcgctagcc
    42241 aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac tttcttgaat
    42301 tatatcagaa aaaaaaacga aaggctagtc gtttcataaa aaaacaatcc agatggccta
    42361 aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac
    42421 aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc
    42481 taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcttc
    42541 tactttcgga agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt gtttcatttc
    42601 tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga catacacatt
    42661 gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga
    42721 agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac
    42781 aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat
    42841 gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg
    42901 tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc
    42961 ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc
    43021 cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac
    43081 ttcgcccatg gcgttttgta accagtaacc gatagatcct tctgtcattg caacacatgt
    43141 atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt
    43201 accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt
    43261 cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt
    43321 agacaagatg gcattgcccc ctaaagcgac aactactttt cgtttggcca tttcattcgt
    43381 cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt
    43441 ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat
    43501 aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc
    43561 ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt
    43621 gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg
    43681 caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt
    43741 gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga
    43801 tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt
    43861 aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc
    43921 ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt
    43981 gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc
    44041 ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg
    44101 ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac
    44161 cattttttga ctgaatccac ggaattcgat tccgtcaaac atacgtccta gtactttagc
    44221 tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg
    44281 atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt
    44341 ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt
    44401 caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc
    44461 tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct
    44521 ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagactg
    44581 ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg
    44641 accaccacga cctcgtgaca attcacttga attgatttct aacactttga tgccatagct
    44701 acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat
    44761 tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt
    44821 accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa
    44881 gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat
    44941 cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt
    45001 aaatacggta tccaaatgca tcattgcacg gttgttcgga attttgattg ccaacacttt
    45061 ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt
    45121 agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc
    45181 ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct
    45241 tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt
    45301 catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa
    45361 gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat catatagact
    45421 gctagaacgt acttcgatat cttttgtacg aaccccagcc atgattttat ctaccatttc
    45481 ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat
    45541 atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc
    45601 ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtcttcgc
    45661 aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg
    45721 atccataata tctggtgtca aattttccac ttcttgtcca ggccgtttca gtaaaactgt
    45781 ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct
    45841 tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc
    45901 attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa acgtttttct
    45961 atttttaagc gtaatttatt cattttttat tctgtataca ctttcaaaaa aatatttatt
    46021 cattgaaaaa atttttttga ataaattaaa aaaataaatt atattatcgt tgttagcgtt
    46081 tactatatgt attattgttg ttttttatac acaaatcatt cgcttcaaaa caaattattc
    46141 aataccattg tatatttttt acataataac aaattatatt tgtaaagtat taaaacttgt
    46201 ttctaatgtt ggtatgttaa aactttacaa attctttaaa aaaagtgaca ataaatatag
    46261 tgaacaataa ggagaggact ttttaatgcg tgttaataaa atcgattttc acttttcaaa
    46321 actcagatat caacctgatt ttgaacattt ctcggatgaa gagttccaac agatcaagga
    46381 acatactgtt cttcgttctt ataaaaaagg acagatcctt ttcgacgatg gtgatcccag
    46441 acaacggttg tatatcttga ccaaaggact cgttcgttta gaacgttatg atgaaagtgc
    46501 aacttattac tactatgact atgtgaatac aaataacctt tttcctatgt caggaatgtt
    46561 tggagaagag acctacactt atacagctca agcgatgact gatattgaaa cattctacct
    46621 tcctgtttcc tactatgaaa aattagcacg aaaaaatccc gaacagttga tctatatgat
    46681 acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt atccaaatcg gattgacctc
    46741 tagcgccttt gaccgggtaa aacaaaattt attcattttg aaagaagaat tgggcgtacc
    46801 aacaaaagat ggtcatatca ccattcctta tccgatcacc ttaaaagaat tagcagtgaa
    46861 tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa caactacgtg attccgggct
    46921 gttagattat gacaaaaagc gttttacttt ctgtaagaca gctactactc cagctatgag
    46981 agaataacgc gaacaaaaat aagcagtgac cctgataaat atcagggtca ctgcttattt
    47041 tcctccctaa aacttctgaa agatccggca gttttagatc actggacaag actcaaagat
    47101 tttctatgtg tgtttcagtt atgtaatatt cactgtttgc aaaaaatact gttagattaa
    47161 ctagatgaag cgtcttgtcc tcatccgatc tagattattc gatattcctc gacaataaac
    47221 gattactcag tgagggcaat cagaggaacg gataatgata aataagctgt aaaaaatagc
    47281 aaaataacaa taatgattaa aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca
    47341 gccctatttc gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc
    47401 ataaacatga ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc
    47461 ttacgaactc ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca
    47521 ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcagc
    47581 tttttcacaa cctctttatt cagataaaac caagcttgga tcagcattca actgatagct
    47641 agcaaaattt cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc
    47701 tccacaaagt cgaagagggg gaatcactag ttctacctca gggagtttag ctgccaaagc
    47761 atcctgcaat ccttcacgca acccttgatt ggccgctaca cctccagcta taatcagttg
    47821 cttgactgga taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact
    47881 tgcttgaaag ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt
    47941 atgcaccaaa ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc
    48001 atggatcatc gctcgcggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat
    48061 ttcttttccg cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc
    48121 tgctgcgtca tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac
    48181 taattctgta tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac
    48241 cagacgggct gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg
    48301 tgcccaagca aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg
    48361 tccataagtg acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc
    48421 atcttccaag cataaggtga tttgttctac gtgatggcgg ctagctactt caggtactac
    48481 gccaccaaaa cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt
    48541 tccatttcta acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa
    48601 ttcttcattc atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc
    48661 gtcggattgc gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa
    48721 agttttcttg ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt
    48781 atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa
    48841 actgcaacag caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag
    48901 ccacagatct ggttatcttc agctaaaagc agataatcag agtgaggctg ttggatgtct
    48961 gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc
    49021 catacgcatt ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct
    49081 cactcatttt ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat
    49141 ctttttttga tcgttgttgc ttgaaacccc aatttccgat aaagtctttg tgcatcttga
    49201 ttactcatac gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg
    49261 atttcgtcaa taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata
    49321 ttcgtgatat ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca
    49381 ttatcaaaga tacctagata tagatgagga agcggtgact tcaattctga tataaaggca
    49441 ctttttgtcc atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg
    49501 atatcttctg tagtcaattc tcttagtacg taacgacgat cgatttgttc gactgttttt
    49561 tctgtataaa tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac
    49621 ataactttcg tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctactcgttt
    49681 taaatagcga ggaagaaaag cttgtttctc tgcaactggt tcaactaaag cacctaattg
    49741 agccacagtt acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg
    49801 caatatttcc tttatttcag agaagaattt gtgacaatca ctcccgacga aataaatagc
    49861 ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag
    49921 tacagtttct aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg
    49981 agcatcaaac atgggcacaa taatattatc cacaccgatg caattagccg cgatggtttt
    50041 cagactagat actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt
    50101 tacacctata cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat
    50161 gtcttttgga cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac
    50221 actgtggttg cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac
    50281 accgatcgcc aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct
    50341 ttctgctatg acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat
    50401 accaaaggca aaactagtac aataatacat gattatctgc tggcgggcaa attttgcccc
    50461 ttcctgaaac acaaaaaagc tgcggtatca ctccattttc ccacagcttt tccatctttt
    50521 atttcgttga ttctcgaata atcaactctg tttccacaat aatctttctt gaaaccggtg
    50581 ctttggaatt acttaattga agaatcgtct ccactgctag ctctcccatc cattcggtat
    50641 aaacttttac agtagaaagc gagggcgtta catatttcgc tacactgaca tcattgaagc
    50701 ccatcacaga aatatcttct ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc
    50761 caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta
    50821 aatattgctt cactgcttga tatcctgact cgacagaaaa atctgcttcg ataattggac
    50881 cttcgtcgta gcctttttct gccaaaactt gttgaaatgc cataattctg ggatctttta
    50941 gccgctgatg gtttttcttc gtatattctg ttccagcaag catagcgata tgttgcgctt
    51001 tatgggagac aaagtgttca atgacttttt gaacacttga cttaaagtcg acaactagag
    51061 agtcgatccc ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt tgggatcctt
    51121 tcatttctaa taactgagct tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa
    51181 gatcactaat ggaatcccac gtttctttca ataaagtaac gtttgcttct tctgcctttt
    51241 tttcgattcc taggcgaata gataaataat aaagatcttc aagctcttcc gtatcgtcgt
    51301 accactggat caggcgaagc gtttgcttag tatttgctgc atttttacga tgtttcgtat
    51361 aatttaagcg ttctgctgct tcaaaaattt tttgctttgt ggctaagcca acggaaagat
    51421 caggatcata gttgagaact cttgaaacag tagcaggaga aactcctgcc aactgcgcaa
    51481 tgtctttaat tgttgccatt tgtctctcct ccttttttac gtttataaaa tcgcagtaaa
    51541 tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat actccagcgt cttccagtac
    51601 acgtgcaaaa acctgaccta cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa
    51661 atcaggatgt tcttttttta attgttctgc ccacggacga tgatattcag ccatctggtt
    51721 ttcttctcct aataagaaat gctctacttc tactagttct ggtttcaaac gaggtggtaa
    51781 aacagcaagc cccattactt cgatcaaccc aatgttctcc tttttgatat gttggacatc
    51841 tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa acattgttgt ctcgaaggac
    51901 aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg
    51961 tgtaccatct gctgaaaaag ctctgataga tactgtttca tccgaatatg ctttccattt
    52021 ttccaatact gagttagctg cttcaataag caagtttttc tctgttgatt gcaaacggat
    52081 cacagacatc ggccatttca cgactcctgc gatgacttgc ggataatctt ctagtttgat
    52141 aagtttttct atcggtgctt ttgccatagg aaattcatga cgtcctgcct gataatgatc
    52201 atgagaaaga atcgatcctc caacaattgg taagtcagca ttcgaaccaa caaagtaatg
    52261 aggcaagact tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg
    52321 gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa taagcataag gggaatattg
    52381 gaatccccag ctttcaccgt ctagattcat tcgaataatc cggtgattgg tgcgggctgg
    52441 atagttcaag cgacctctat atccttcgtt ttccatgcaa agcatgcagg taggatagtc
    52501 tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg gcttagataa
    52561 attgattgtg atttccagtt caccatactc cgactctgcc ggaaatttga tgttttttgc
    52621 gatggctctt gttttgatgt agtcattttt cttacttaaa ttaaagaaat actcagttgc
    52681 ttcctctggg cttttcgaat agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg
    52741 tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt tctgctggca attctgaaag
    52801 aacaccattt tctcttgcct tagcgacaag ctgatccaat aagtctacag attcttcctt
    52861 aactggtctt acttcaatgc tttctagagc atcttctcct accatcccta aaagacgatt
    52921 ctgtaaatat aagcggtcca tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa
    52981 atcacaaatc gtttgactga ttgacatatc tcattcccct attcttcata accatttgga
    53041 tggctgacat gccaatccca tgctgttttg ataatcgctt tgatatctgt gtattcaggt
    53101 ttccagccca gaatctctct cgctttttca cttgaagcga ctaatcggct aggatctcca
    53161 gctcttcttg gtgctatttc tgctggaatt tcttttcctg tcacttctct agctgcttct
    53221 agcatttcct tgactgagta tcctttgctg ctgcctaaat tgaagaagtt gctttcattt
    53281 ccttctttca agtattccaa agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca
    53341 taatctctga tacatgttcc atctggtgta tcataatcgt ctccataaac agccaaggct
    53401 tttctttgac caagagctac ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga
    53461 tcttctccga tagatgcatc tgcttttgct ccggctacat tgaagtagcg caaagcgaca
    53521 tagcgcatgc catatgcttg gtcacaccac ttcatcattt tttccatcat caatttactt
    53581 tcaccatacg gatttttagg attagctggt gtatcttctg tgattggaga ctctttcggc
    53641 tctccataag ttgctgcagt agaagagaat acgattttat tcacgttgaa ctcgtgcatg
    53701 acttcaagta agatttgcat accgtatacg ttgttgttga aatacatcaa tggtttttcg
    53761 acagattctc ctactaaaga gcttgcagca aaatggatta ctccttcaat cggttccttt
    53821 tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc cttcataaaa atgagcatct
    53881 ggatgtactg cttgtttgtg acctgtgagc aaattatcta ccacagctac ttgatacccc
    53941 ttttgaacta gctggtctac agcgtgcgaa ccgatataac cggctccgcc tagtactaaa
    54001 attgtcataa aaaacactcc tttgattagt aaaattgtgt ttgttgattc tatataattt
    54061 aaaaaacgaa gtttactttt atggtgaaaa caaacaaagt aaaatttcgt aaattcatat
    54121 ggtaaattta taataaaaca tttagtaaac attttcaagg gttttttgca aaggtttaca
    54181 aacaaacgta aaactagcct ttttttctct tttgttccat aatagatata acaaataata
    54241 aatgaggtgg attcaatgaa aaaatttgta tctggaatat tagtaggaag tctggcaaca
    54301 gccgctgccg ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga tccaattgac
    54361 gaaaaagaag caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg cgtttctcgt
    54421 taataattat ttaaaaatac gcatgaagac tttctaaaaa acagataagt catcatgcgt
    54481 atttttattt gtcagtcaat ataagttgtg ctattttatc tgcctgtctc agattccatg
    54541 gatctgtata aggtctagct actgctgcag ttagccaatc cacaccttca acaggtattc
    54601 catagcaata aaagtcagac tggatttccc ctgaagcatc aatcacgcga ttcgtacctc
    54661 gctcaacatc tatagcttct gaatgataac ttcctgtatt atctgaaaat gaaaaagggc
    54721 gaacaagttt gttttccttc aaatttttta tgacaggatt acttgaacga gaaaagcttg
    54781 tttgtggcaa tcgagcttca atcaaatagc gactgctgat ttctttatct tgtagagaaa
    54841 taacatagcg gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa tgaaaaatac
    54901 ccgcatctat cagtgctgct aattctttcg tccgaatgat tggcggaccg atcgttaaaa
    54961 agctatttag atgagtaaac catccccata gtatttcttt gtattcctta gcagaaaaaa
    55021 cgtgccattc aatagcctgc cggattggat cacgccagtc ttttaatgca tcaaatgttg
    55081 aagcaaatgc tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct tcaataaata
    55141 ttcggctagc ttctgaaaag ggctcattct gataggcaag aggagtttct aaatacgacc
    55201 aatcccatct ataagggatt agtgctggat gctttccgag acactcctct gaagagaggg
    55261 agagaaaatc atgctcaaaa tttttctttg gaatcggcaa gtgatattct tcgatgattt
    55321 tcttgtaata aaacaactca acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca
    55381 aaaatcccga ctgatgccat tgctccaggt tttcttttgt tagtaaccgt ggccatgcca
    55441 ttgctccgcc ttgcttttga tttcttcctc gagggtaata agggagcccc cttcttgatc
    55501 cactgtatac aatcggttcc ttgcctgatg gatgatagac cagttttccc ttttcgttct
    55561 cgaagcgccc tcctcttgat actgtaaaca atccgacata atcaaaaaaa cttaagccta
    55621 gacctcttaa aaagacattt tctttaggcg gtatcttcga aagatctaca tctgcaggat
    55681 tagaaggaag ctgatacagt aaaacatttt tctttgcgta gtctacccac actctttctt
    55741 cttctgtctc atcattttcc cagtgaccgc ttgctaaaac aagctgatcg atagaatagc
    55801 tttgctgatc tgtctgtaaa agataaccat cttttctttt gcttactttt tttactaaag
    55861 aataaacaaa ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt
    55921 gatagagtcc gtataaacat cgagtagatt gctgatcttt ttccagacga ttgatttctt
    55981 ctataattag ttgcttattt ttggttaccg tctgttcgat ataatctgta gcaggtcctt
    56041 tactccattg aaacaagttg ggtccctgtg ttaccaatcc tccactggac aatgtttcat
    56101 ctgtaaaaag cgtcacttgt tgagaaacag aattcattaa taactccggt ggctgattca
    56161 accgccaaac tcgacctcca ggaccattgg gatcgaaaag aaaaagttca agagaatgtc
    56221 tgtcctgatt attatggaga atcctttctg ctacacttaa tcctctaggt ccggcaccaa
    56281 tgatccctat tttcatttgc atgcctccat ttcgataaat tttttcaata aaacagcatg
    56341 gttctgatgt tcattttttg caccataaac aaaagtaatg actggatgtt ctttcataag
    56401 ttgactgagt ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt cttgctggta
    56461 tttctttgca aatgaagcaa atcgttctgg tacatggtca aaagttttgc gtgcttcatt
    56521 actaggtgca atttctttca accacagatc taactgcgct ttttcttttg aaattcctcg
    56581 tggccagaat ctatccacga gtattctata gccatcctct ttttcagcat caaggtaaac
    56641 acgttttaat ttgatcatag ttgtttcctt ctttctacct tttcgcttag cttatatatg
    56701 tagataatag cacaaaataa aaaaaatgaa cggcacaact atccattgcg ccgttcatgt
    56761 gatcttggtc aaaatctaat gattgattcg gtttttaaag ctgtcataca atcatctgtt
    56821 ttatactttt ttacttttga tttttccaga ccatccgtca tatccaccct taagtaaata
    56881 aatctcttga taacctgctt tacgtaattt atttgctgta cggatgctca aacttttctt
    56941 ttgatcgtaa atatagacag gctggtcttt tcgaatagat cctaatgaat ctttcaaaac
    57001 agtgtaaggg atgtttcttg cacctaaaat atgcccagca tcaaacacat ctttttcgcg
    57061 aacatcgatt acttgtgctt ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt
    57121 tgctgaacgt ttggccatta ctcgtagata gacttcccaa aggatgattg caagcacaat
    57181 caatagcaaa atgatattaa ttacccataa aaccatgtaa tgcctccgtt tttttaagca
    57241 aatttcaagg ctaatgaggc agcaccaatg acaccagctt cattaccaag ctctgctaat
    57301 ttgatttttg ttgattctct tacttgcggg aaagtaaatt ctttaaaata tttctctaca
    57361 cggctacgta aaaattctcc tgctgccgat actcctccac ctaacacgat acttgacgga
    57421 tttaatgtat tccccaagtt tccgcaagct aaacctaagt agaagcatac gcgatcaaca
    57481 accatcaatg caaacggatc gtcttcttga gccatttcaa agatatcttt acttgttact
    57541 tcttctccgt tgtctagcat tgctttcagt tttgaatccc ctgcgtattc ttctgccaag
    57601 aaacgaccta ggcgtaccac acctgtagcg ctagatacag tttctaaaca tccgcgttta
    57661 ccgcaagtac attcgaaccc ttcaggatcc acagtgatat gtccgatttc tcctccagct
    57721 ccgctcacgc cgtgaatcaa atttccgcca gcaacgatac ctccgccaac acctgttcct
    57781 agagtaacga atacaacgtc tggatcgttt tctccagcac ctttccatcg ttcacctaaa
    57841 gcagcaacat tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc ctcgatcagt
    57901 tttttcacaa attgaacttc tgtccagttt aagttatacg caccaataac cgttccagct
    57961 tcgctgtcca cgcttcctgg ggtacccatg ccgattccga taaagtcttc ggctttcata
    58021 ttatataaat tcaaacgatg attgattgat tcaatgattt caggaacgat atgcttacca
    58081 tcatccaaaa tattggtatc gatgctccat ttttgctgaa tctctccttc tggtgttaaa
    58141 attgcaaatt ttgctgttgt tccacctaaa tcaatcccaa taatcttctt gtccattaca
    58201 aatttccttt ctttcggctt tcttcaattc ggtgctctct ttttaatatg cgccacgctg
    58261 tcatgtatgt atcatgatca atcaaacggc tatcatacag attacgcaat tcgatagtca
    58321 ttaattcaat gtcatagatg cgtgcaccca catagataaa gatgccaaat tttttgaaca
    58381 gctgctgtac atcatataag ctctccatct ttaagcactt ccttcatatt atacaagtaa
    58441 accatatttt tttaaaccca gtaaaataca tattacaaaa agaaatacaa aaactagtgt
    58501 agaaagaatc ctttggtgta tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt
    58561 ccctaacact aatccgccaa ttagcccacc gacatgtccc caaacatcta cagaggtatc
    58621 cagaagacca aaaacaaatg tcaacacaat aaataaaaga aaatgtctga ctagctgctg
    58681 gatcgctggg ttatgtttaa aatgaaaccc taagataaac aacgcaccga acaaaccgaa
    58741 tatcgaagta cttgcaccac cagacagaac attcgcttcg ttaaaagcaa agcttgctgt
    58801 atttcccatt atcccgctga acatataaat caagaaaaaa cgccaatggc catagattgc
    58861 ctcgatttgc tgccccataa aatacagtat cagtgagttc attacgaaat gcatcagtcc
    58921 aaaatggatg aagattggtg tcactaaacg ccaccattca ttgaagtgaa caagataagg
    58981 accatacatt ccggcataat aagggatctc aagtcgcgga aataattcca tcatgaggaa
    59041 gaccgctgtc tgtatcccta aaaaagcata ggtccataac ggtcgtctta accatgcacg
    59101 caattttatt tgctgttgat aattcatgaa aacctcccat ttcattttag tcaataaaca
    59161 attgttggat cttctgatca aagctctccg gttgccattg ttcattcaat tgttcacgaa
    59221 aaacaaggct acatgttttt ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc
    59281 caaaaccgat tcgataaccg gctctattgt agataatacc tggtacaatg aggagatcga
    59341 tcatctccgg caaatagagc ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta
    59401 caagaacgga ctgttcatcc atttcataaa acgccatctg acgtttcggc aaagttttag
    59461 gtacaactat tttcttttgt tctttttctg ctcgttggat aaccggctgt gtatccagtt
    59521 caaattcatt cgataaagtc gtccctatca cttttgctga cttccagtac tcgctggcga
    59581 atagctgttt atagattcgc tgttcttttt cctttttctt gccacttttg gccagatttt
    59641 tcagcttttc gattcctttc cggcgtaatc ttactttcaa ttggtccaaa aactcaccct
    59701 tccttctctc ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa
    59761 atgcataaat agaataaaaa gaagacctag aaatgattgg ccttcctttt ttaatgaatt
    59821 tcaactgttc agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat atggcgcacc
    59881 aaaaagggca atcaaaattc ctgtaggtac ttccccactt ggtagaatca ctctagcaac
    59941 catatctgca ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt
    60001 attttcttta cgtacgacta gcttcgcagc atgaggagct agtagtccta caaaagataa
    60061 actacctgtc accgctacac taccgcttgc caaagcaaca gctaacaaca aaaggatttt
    60121 ttgactttta gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa
    60181 tacaatcctc tgtgcataaa gtcctcctag cggaattaag ataaccgccc atggaagcaa
    60241 tgcccaaaca taaggccagc tcgcacccca gattgatccc gctaaccatg cattcacaaa
    60301 tccataattt tccttagata attggatcgt gcatagcaat gttaatgcag aaaggccggc
    60361 attaacggca ataccggaca ataagattcg attcataccc aaaaagccat ttcgatcata
    60421 ggccgcaaga tagacacagc aagcagctaa aacaccacct agaaaggcta caactagcaa
    60481 tagaaacggc agcgaatagt gcgaaaagaa acctaaataa agcatcacta aaaatcctgc
    60541 tccggcatta atgcccaata tactagaatc tgccaattca tttcttgtta ctccttgcaa
    60601 gatataacca gaaacgccaa gacaagctcc tgctaataaa ctgatcagta atcgaggaac
    60661 tcgaaattct ctaacaatca gcgccaaaga agatgattct ttccctatga acgtggcaaa
    60721 aagctgatcc aggtaaataa aaggcgtacc aatcattaaa ctacctatag acaggatgac
    60781 aagtacaaaa agcaagaaat agaacgtcca atatattttt ttcaccgttg ttccctcctt
    60841 acttgtatca gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa aggtgtttca
    60901 aaagggggat tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc accgccaact
    60961 aaaaaagaga caggtagaac ataacgatag tccgttccaa ccattccgcg aacaactggc
    61021 ggaaccaaca gcccaataaa tgaaacaggt ccgactaacg aaacggcaat ggctgctaac
    61081 actgcaacta gacagaaaac aatcaaccgc gttcgtttag gatgacttcc aagagctaat
    61141 gcatgtgttt cactcaaatg aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa
    61201 acagcaagta tatataaagg aaacaatact gctaattgtt tccatgtgat attggcagct
    61261 ccgcctacaa accaaaaagc aagatcctgc tgcagattaa agaattgggt aagtgcttgg
    61321 ctgatagcag taaaaaaaga actaatagct acgccagcta aaatcagctg taaaggattc
    61381 atgcccattt tcgaaaactg caaaaaacca aatacgagca aagttacagc aaaagaacct
    61441 gccaatgaca ttagaaacac agaaaaatca gaagctttag gccaaaaagc aaaactaagg
    61501 gccattgcca acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc taaaggattt
    61561 cttgtcactc cttgcatcaa tgcaccgctc aatgcaaaac aactccctac aagaaatgct
    61621 cccgccacac gaggcaaacg aatatttcgt atgatttggt gactttgttt tgatctatca
    61681 aaatcaaaca acgcctgcca aaaatctgct ttttccacag tcgctgcgcc atttgaaaga
    61741 gcaacaaaaa aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct atttttcttc
    61801 atagtcctca taattgatcc tcctgctctt ttggcaacaa atcataggtt agaaaaactg
    61861 gtttatttgt tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac
    61921 actcttttgt gatcacttct tcaggtgtac cttctgtgat caacttaccg tttttcattg
    61981 ctaataaata atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac atcaagatcg
    62041 ttgtttgttt catttgattg atttcagtca ataactgtag gatctctaat tggtgtgccg
    62101 gatctaaaaa tgttgtcggt tcatccaaga tcaagatatc tgtttcctga gcaagcgcca
    62161 tagcgatcca gacacgctgg gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa
    62221 atccagctag acctgtagca tccaacgccc actcaattgc ttcatgatct tgcgtgtcca
    62281 tgccactgaa cagtttttga taagggtatc gtccgtaaga tactacttct tctactgtca
    62341 tacctgtagg gtgttcacta gtttgtgcta ataatgcaat tttttttgca agttcttttg
    62401 aactgtatgt agagatatca gagttattga caatgatctt tcctgatttc acaggtagta
    62461 tacgggtaaa acttttcaat aaagtagatt ttccacttcc atttggccca atcaaactag
    62521 tgattttccc ttcaggaatc gtaacagaca tgttttcaat gatttttttg ttttcgtagc
    62581 cggctgcaat gtcagctgct cttaatctcg tcatttcacc catcctttcg ctgaaaatca
    62641 ttctcaatta tgacgctgtt tcaacatttc gtcaagcgct actttcatat ctatagccaa
    62701 atgaagcggt tgacagcgtt cgaaatatca ggaataataa ccaatgagaa agattctcaa
    62761 ttatgttagg aggaaagaaa tgaaattaaa aacaaaagta ttcacattat ctagcatatt
    62821 gctgctggct ggagtactga gcgcttgctc cccctcttca gataaagaaa cagctacaac
    62881 tgccaattcg acgacttcat ccaatagtac tcatacagtc acagatacac ttggacataa
    62941 agtagagatt ccaaacgcgc ccaaacggat catcggcagt tacttagaag actatctgat
    63001 tgcactaggg gaaaaaccta tcgcccagtg gactgttggc agtggttcca ttcaagatta
    63061 tctgcaagat gatttaaagg atgtcccaac gatttcttat gaccttcctt atgagaaagt
    63121 gttaagcttt gagcctgatt tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa
    63181 atacgaacaa tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac
    63241 atgggaagaa caactaaaag atgtcggcaa agctttggca aaagaaaaag aggcagaaaa
    63301 aatcatttcc gattatcaaa aatccatcaa gaaaactcga gaagaactag cagataaaat
    63361 cgagaacaag acagctgcag tgttgtgggt gaccaacaac tcagccttta tggtcagcaa
    63421 aaatcggtca agtggaagaa tcgtatacga cgatctggaa tttggtgttc ctgatctagt
    63481 ggaagaggtc tccaaaaaag ccacttctga ctggtctgct gtttcctctg aaaagctagc
    63541 tgaattagat gctgactaca tcatccttgt gaacagtgac aaagatgctg ccatgttcaa
    63601 tgaaccaagc tggaagaatc taaaagcagt gacagacaat catgtactgc aattcggacc
    63661 tgaatctagt tggctatata acggacctat tgccagtcag gaaatggtca atgatatcaa
    63721 agaaaaacta aattgatttt aactaaaaaa agcatcttca cgattagtcg tgagatgctt
    63781 tttttcttat taacaaacta gacgcataga ttttattctt cttttgaagc aaccctattt
    63841 tttactcttt cccatttttg aaaacgatgg gcatatagat tttcttgatc cgttatcccc
    63901 tcatactcct ctataagggc gaagtctgaa aaatcaatat tcccaatata cgtatcgcct
    63961 tcaaacgtgc tatcgatcaa tgttcgccag atccgaccag tgtaaggaag aagttcttga
    64021 aaaatccgac taccacccga aacatagata tcttctgaga tcgtttgact atacgacaaa
    64081 agttcatcga tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt tggttgcgtt
    64141 gtcaacacga tgatatggcg ataaggaagt gaaagatccc ccattccttc ataggtcttt
    64201 cttcccatca ctaaaattcg atccattgta tgttcgcgaa agaaacgcat atcatttggc
    64261 aatcgccatg gtaacaaacc atttttgccg atcagaccat ttttatcttg tgcccacatg
    64321 gaaataaaca tattatccgc tccttatgat tttagatgtt ttgagataaa aaaagtctca
    64381 attctttgac cagcaaggaa ttgagaactt taaaaacatt attttgtttc acggtgcaaa
    64441 gtaactttgc gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg attgttacgt
    64501 ttgtttttgc ttgttagata gttacgttct ttacaagaag tacactctaa agtgatgttt
    64561 acgcgcatgt ttttccctcc catttttaca agaatcctct aagatgactc tcagacttga
    64621 ttatcttacc atgatttttt ctagattgct agtgtttttc acgattttgt taagtaaaac
    64681 tacttgacga taaatacaac ggttttactt ctcaaacaaa atctttttct ttttgtataa
    64741 atacccttac acttgtttat atagattatg ttttttaata aagggggata ctaatatgcc
    64801 atggaacatg aaggactttc ctgcatcaat gaaaaactta gataaattga caagaaaaaa
    64861 agcaatcgac atcgcaaatg cattattaga tgaagggtac cctgataacc gtgcgatccc
    64921 aattgccatt gatcaagcaa aaaaatggta tgaagatgct agtgaatcag aaaaacgaac
    64981 tttcgaaaaa gaaaaaaatc caagcaagac ggatgaacac gatacgaatc ctcgtgctgg
    65041 aaaactgctt gattcagatg tgatcgtaga atatgaggaa gaacaatgga tcgtgaaatc
    65101 taaaggggca aaaaaggcaa gcaaccattt tgatacaaaa aaagaagcaa tcgaaaaagg
    65161 aaagcaagta gcacaaaaca aagaatcaac tttgttcatt tataaaaaag atgggacaaa
    65221 agaaaaagaa atcgattatt gaacatatgt aaaggggttg tgacgaaaat cgtgtcacaa
    65281 cccccttatg ttcgaaacaa tggtgttcct ctgccaactc cgaaccataa gtcataatat
    65341 ctataaaacg ggaagagctg ttttttgaaa ttgtttctta tcactcagtg ttaaacggta
    65401 ggagaaaacc tcttttttat tgatatttga ttttcatcaa acagtcacat agtcgattgc
    65461 ttctgcttgc ggaatcgttt catctacaaa gtagacttta taccatgtca agaaactgaa
    65521 gattgtccag atttttcttt gctcttcttt acgtccttcg tgatggtcat ctagcaactt
    65581 catgattttt tcttgatcaa aaaattcttt tgcaaaatct gcttcgaata atgctttgac
    65641 ttgttcgtag ccgtgttctt ctttcaacca agctttgatt ggcacaggga atcctaattt
    65701 cacacgattc gaccattctt ccggaagatg tttgttcgca gcttgtctga aaatatcttt
    65761 tgtgttgttt tgattcaata aaaacttact tggtatttgt tgagccaatt tcatgacttc
    65821 gatatccaat aaaggtactc ggacttccaa agaacttgcc atagaaagtt tatctgcctt
    65881 taacaagata tcttttggca tccattgatg aagatcgaca tattgcatct tattgacttc
    65941 atctttgatt ccttctgttt tctcgtaatg aacagtcatg atttcatcca cagatggtgc
    66001 tgtttgatat tttggtgtta acacttctga tgcttctgat tcttcgaaca ctcgagcatg
    66061 accgataaaa tattcttttg caggagcaag tgattcatat aaatggatcc gtccatggaa
    66121 gtttttcatt tttccgattt tacgtcctaa attatagcgt gttccttttg gtaatttttt
    66181 caatccttga gcaatcacac ggatgaattt tgaattcgta tggaatccat acgtttggta
    66241 tcccgcaaac agttcatccg ccccttctcc agattggact acccgcacac tttgtgaagc
    66301 aagatttgct aaaaagtaca aaggtacgca agaaggattt gaatcaggtt catctaagtg
    66361 atactggatc aaagggaatg ctttgaacga catgtctccg tcgatcactg ctgctgtatt
    66421 atctaagtcc aatagttcgg ttagttttct ggcttcaatc gcttcattat aggttttatc
    66481 atcaaacccg attgagaaag aatgatctgg tttcaataca gaggttacat aactggaatc
    66541 aaccccgctg gataagaaag aaccaacttc tacatcactg accgtatgtg cttcaataga
    66601 agcttgaacg gtttcgtcga ttttttcgat ccattcttgt cgggaatgcg tttcttttgt
    66661 ttcaaaatct gcatcccaat attgctgaat gtctattttt ccatctttat acgtataata
    66721 atggccttca ggcaaacgat agacatcttt gaagaatgtt tcgccgttta atggagaata
    66781 ttggaaagtc atataaggtt tcaacgcttc tttgttcaat tctttgttaa aatcaggatg
    66841 aggcagaaaa cttttgattt ctgaaccaaa cataaatgtt ccgttcattt ctgcataata
    66901 atatggtttg atcccaaagt ggtctcttgc accgaataac tcattctttt cattatccca
    66961 aatagcaaaa gcaaacattc cgcggatctt ttgcaataat tctgttcccc attcttcgta
    67021 accatgcaaa aggacttctg tatctgcatg tgtttgaaaa acgtggccag ctgcaatcaa
    67081 ttcttctctc aaaggctggt aattatagat ttcgccatta aaaataatga tctttgtccg
    67141 atcttcattg taaataggct gagtaccgcc ctctaagtca ataatactta atcttctgaa
    67201 tcctaaagca acatgtttat cgatatattc tcctgaacta ttaggtccac ggtgaacgat
    67261 tcgatccatc attgtattaa taattgtttt tttgttgtcc ttgtcgttaa caaaacctac
    67321 aataccgcac atttaacttt cacatcctaa tataaaatta ctgtttattt cattcatatg
    67381 catcttatca tagaataaat agcggacgaa tattttctct cttacaattt cgtcacgtga
    67441 aaacaaaaga tgaaagtttc tgtttttaca gaaaataaag tcccaatatc taatttacca
    67501 tgttttcttt tttgaggcta ccttattctc ttcgtgttaa aaaaatatta gtggatgcta
    67561 tttccaaaaa aaccagcaaa aaaagacacg acctaaagat cgtg
//
LOCUS       DACEOM010000004        65712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEOM010000004 DACEOM010000000
VERSION     DACEOM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.004"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            197..715
                     /locus_tag="IW781_000209"
     CDS             197..715
                     /locus_tag="IW781_000209"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1181232.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            843..1526
                     /locus_tag="IW781_000210"
     CDS             843..1526
                     /locus_tag="IW781_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1181233.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1835..1908
                     /locus_tag="IW781_000211"
     tRNA            1835..1908
                     /locus_tag="IW781_000211"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1869..1871,aa:Arg,seq:tct)
     gene            2067..2426
                     /locus_tag="IW781_000212"
     CDS             2067..2426
                     /locus_tag="IW781_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1181234.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2549..4180
                     /locus_tag="IW781_000213"
     CDS             2549..4180
                     /locus_tag="IW781_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1181235.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4242..4883)
                     /locus_tag="IW781_000214"
     CDS             complement(4242..4883)
                     /locus_tag="IW781_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1181236.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5165..5473
                     /gene="rplU"
                     /locus_tag="IW781_000215"
     CDS             5165..5473
                     /gene="rplU"
                     /locus_tag="IW781_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1181237.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5500..5844
                     /locus_tag="IW781_000216"
     CDS             5500..5844
                     /locus_tag="IW781_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1181238.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5857..6150
                     /gene="rpmA"
                     /locus_tag="IW781_000217"
     CDS             5857..6150
                     /gene="rpmA"
                     /locus_tag="IW781_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1181239.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6557..9391
                     /locus_tag="IW781_000218"
     CDS             6557..9391
                     /locus_tag="IW781_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1181240.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9582..10073
                     /locus_tag="IW781_000219"
     CDS             9582..10073
                     /locus_tag="IW781_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1181241.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10176..11165
                     /gene="manX"
                     /locus_tag="IW781_000220"
     CDS             10176..11165
                     /gene="manX"
                     /locus_tag="IW781_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1181242.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11198..12001
                     /locus_tag="IW781_000221"
     CDS             11198..12001
                     /locus_tag="IW781_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1181243.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            12022..12936
                     /locus_tag="IW781_000222"
     CDS             12022..12936
                     /locus_tag="IW781_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1181244.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            13019..13393
                     /locus_tag="IW781_000223"
     CDS             13019..13393
                     /locus_tag="IW781_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1181245.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13532..13777
                     /locus_tag="IW781_000224"
     CDS             13532..13777
                     /locus_tag="IW781_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1181246.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13949..15013
                     /locus_tag="IW781_000225"
     CDS             13949..15013
                     /locus_tag="IW781_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1181247.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15259..15684
                     /locus_tag="IW781_000226"
     CDS             15259..15684
                     /locus_tag="IW781_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1181248.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15677..16126
                     /gene="nusB"
                     /locus_tag="IW781_000227"
     CDS             15677..16126
                     /gene="nusB"
                     /locus_tag="IW781_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1181249.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16223..17077
                     /locus_tag="IW781_000228"
     CDS             16223..17077
                     /locus_tag="IW781_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1181250.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17078..18415
                     /locus_tag="IW781_000229"
     CDS             17078..18415
                     /locus_tag="IW781_000229"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1181251.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18420..18644
                     /locus_tag="IW781_000230"
     CDS             18420..18644
                     /locus_tag="IW781_000230"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1181252.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18646..19536
                     /locus_tag="IW781_000231"
     CDS             18646..19536
                     /locus_tag="IW781_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1181253.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19545..20363
                     /locus_tag="IW781_000232"
     CDS             19545..20363
                     /locus_tag="IW781_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1181254.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20470..20919
                     /locus_tag="IW781_000233"
     CDS             20470..20919
                     /locus_tag="IW781_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1181255.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20937..22619
                     /gene="recN"
                     /locus_tag="IW781_000234"
     CDS             20937..22619
                     /gene="recN"
                     /locus_tag="IW781_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1181256.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22674..22790)
                     /locus_tag="IW781_000235"
     CDS             complement(22674..22790)
                     /locus_tag="IW781_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1181257.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22863..23819)
                     /locus_tag="IW781_000236"
     CDS             complement(22863..23819)
                     /locus_tag="IW781_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1181258.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23990..24358
                     /locus_tag="IW781_000237"
     CDS             23990..24358
                     /locus_tag="IW781_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1181259.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24528..24959
                     /gene="mraZ"
                     /locus_tag="IW781_000238"
     CDS             24528..24959
                     /gene="mraZ"
                     /locus_tag="IW781_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1181260.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24980..25939
                     /gene="rsmH"
                     /locus_tag="IW781_000239"
     CDS             24980..25939
                     /gene="rsmH"
                     /locus_tag="IW781_000239"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1181261.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25944..26345
                     /gene="ftsL"
                     /locus_tag="IW781_000240"
     CDS             25944..26345
                     /gene="ftsL"
                     /locus_tag="IW781_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1181262.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26346..28538
                     /locus_tag="IW781_000241"
     CDS             26346..28538
                     /locus_tag="IW781_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1181263.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDRDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28564..29526
                     /locus_tag="IW781_000242"
     CDS             28564..29526
                     /locus_tag="IW781_000242"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1181264.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29540..30901
                     /locus_tag="IW781_000243"
     CDS             29540..30901
                     /locus_tag="IW781_000243"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1181265.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30927..32015
                     /gene="murG"
                     /locus_tag="IW781_000244"
     CDS             30927..32015
                     /gene="murG"
                     /locus_tag="IW781_000244"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1181266.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32030..33250
                     /locus_tag="IW781_000245"
     CDS             32030..33250
                     /locus_tag="IW781_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1181267.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33406..34731
                     /gene="ftsA"
                     /locus_tag="IW781_000246"
     CDS             33406..34731
                     /gene="ftsA"
                     /locus_tag="IW781_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1181268.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34754..35995
                     /gene="ftsZ"
                     /locus_tag="IW781_000247"
     CDS             34754..35995
                     /gene="ftsZ"
                     /locus_tag="IW781_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1181269.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            36012..36689
                     /locus_tag="IW781_000248"
     CDS             36012..36689
                     /locus_tag="IW781_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1181270.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36703..37302
                     /locus_tag="IW781_000249"
     CDS             36703..37302
                     /locus_tag="IW781_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1181271.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37317..37604
                     /locus_tag="IW781_000250"
     CDS             37317..37604
                     /locus_tag="IW781_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1181272.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37653..38435
                     /locus_tag="IW781_000251"
     CDS             37653..38435
                     /locus_tag="IW781_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1181273.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38456..39163
                     /locus_tag="IW781_000252"
     CDS             38456..39163
                     /locus_tag="IW781_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1181274.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39425..42223
                     /gene="ileS"
                     /locus_tag="IW781_000253"
     CDS             39425..42223
                     /gene="ileS"
                     /locus_tag="IW781_000253"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1181275.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42272..43798)
                     /locus_tag="IW781_000254"
     CDS             complement(42272..43798)
                     /locus_tag="IW781_000254"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1181276.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43813..44460)
                     /locus_tag="IW781_000255"
     CDS             complement(43813..44460)
                     /locus_tag="IW781_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1181277.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44644..44973)
                     /locus_tag="IW781_000256"
     CDS             complement(44644..44973)
                     /locus_tag="IW781_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1181278.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45150..45878
                     /gene="sfsA"
                     /locus_tag="IW781_000257"
     CDS             45150..45878
                     /gene="sfsA"
                     /locus_tag="IW781_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1181279.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45894..46907
                     /locus_tag="IW781_000258"
     CDS             45894..46907
                     /locus_tag="IW781_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1181280.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46907..48184
                     /locus_tag="IW781_000259"
     CDS             46907..48184
                     /locus_tag="IW781_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1181281.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48247..50949
                     /locus_tag="IW781_000260"
     CDS             48247..50949
                     /locus_tag="IW781_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1181282.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51101..51418
                     /locus_tag="IW781_000261"
     CDS             51101..51418
                     /locus_tag="IW781_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1181283.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51448..51768
                     /locus_tag="IW781_000262"
     CDS             51448..51768
                     /locus_tag="IW781_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1181284.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51876..53336
                     /locus_tag="IW781_000263"
     CDS             51876..53336
                     /locus_tag="IW781_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1181285.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGANVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53404..53625
                     /locus_tag="IW781_000264"
     CDS             53404..53625
                     /locus_tag="IW781_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181286.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53656..53838
                     /locus_tag="IW781_000265"
     CDS             53656..53838
                     /locus_tag="IW781_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1181287.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53838..54251
                     /locus_tag="IW781_000266"
     CDS             53838..54251
                     /locus_tag="IW781_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1181288.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54374..55555
                     /locus_tag="IW781_000267"
     CDS             54374..55555
                     /locus_tag="IW781_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1181289.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            55944..56017
                     /locus_tag="IW781_000268"
     tRNA            55944..56017
                     /locus_tag="IW781_000268"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55978..55980,aa:Arg,seq:cct)
     gene            complement(56086..57225)
                     /locus_tag="IW781_000269"
     CDS             complement(56086..57225)
                     /locus_tag="IW781_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1181290.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57524..58159)
                     /locus_tag="IW781_000270"
     CDS             complement(57524..58159)
                     /locus_tag="IW781_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181291.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58272..58907)
                     /locus_tag="IW781_000271"
     CDS             complement(58272..58907)
                     /locus_tag="IW781_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1181292.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58941..59402)
                     /locus_tag="IW781_000272"
     CDS             complement(58941..59402)
                     /locus_tag="IW781_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1181293.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59532..59963)
                     /locus_tag="IW781_000273"
     CDS             complement(59532..59963)
                     /locus_tag="IW781_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1181294.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59981..60301)
                     /locus_tag="IW781_000274"
     CDS             complement(59981..60301)
                     /locus_tag="IW781_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1181295.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60600..61376
                     /locus_tag="IW781_000275"
     CDS             60600..61376
                     /locus_tag="IW781_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1181296.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61391..61594
                     /locus_tag="IW781_000276"
     CDS             61391..61594
                     /locus_tag="IW781_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1181297.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61610..61948
                     /locus_tag="IW781_000277"
     CDS             61610..61948
                     /locus_tag="IW781_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1181298.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61935..62114
                     /locus_tag="IW781_000278"
     CDS             61935..62114
                     /locus_tag="IW781_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181299.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(62157..62627)
                     /locus_tag="IW781_000279"
     CDS             complement(62157..62627)
                     /locus_tag="IW781_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181300.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62714..63412
                     /locus_tag="IW781_000280"
     CDS             62714..63412
                     /locus_tag="IW781_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1181301.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63590..63931
                     /locus_tag="IW781_000281"
     CDS             63590..63931
                     /locus_tag="IW781_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181302.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63924..64595
                     /locus_tag="IW781_000282"
     CDS             63924..64595
                     /locus_tag="IW781_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1181303.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64601..65287
                     /locus_tag="IW781_000283"
     CDS             64601..65287
                     /locus_tag="IW781_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181304.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            65290..>65712
                     /locus_tag="IW781_000284"
     CDS             65290..>65712
                     /locus_tag="IW781_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1181305.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKP"
BASE COUNT        21825 a        10992 c        13754 g        19141 t
ORIGIN      
        1 taaaacttgt aatgttaggg taaacgtttt ttaaactaac gtcaattatt tataataaaa
       61 aatgagataa agatgaggaa atagaattag aaacatataa ttattaaagg gaaacatttt
      121 tacagctata atttgaaaaa aatttatcgt tattatatga ttaagaagga ttgaataaag
      181 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
      241 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
      301 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
      361 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
      421 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      481 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
      541 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
      601 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
      661 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
      721 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
      781 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
      841 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
      901 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
      961 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
     1021 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
     1081 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
     1141 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     1201 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     1261 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     1321 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     1381 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     1441 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     1501 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     1561 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     1621 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     1681 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     1741 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     1801 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     1861 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     1921 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     1981 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     2041 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     2101 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     2161 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     2221 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     2281 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     2341 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     2401 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     2461 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     2521 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     2581 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     2641 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     2701 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     2761 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     2821 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     2881 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     2941 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     3001 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     3061 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     3121 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     3181 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     3241 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     3301 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     3361 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     3421 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     3481 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     3541 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     3601 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     3661 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     3721 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     3781 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     3841 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     3901 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     3961 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     4021 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     4081 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     4141 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     4201 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     4261 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     4321 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     4381 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     4441 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     4501 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     4561 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     4621 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     4681 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     4741 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     4801 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     4861 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     4921 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     4981 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     5041 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     5101 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     5161 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     5221 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     5281 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     5341 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     5401 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     5461 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     5521 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     5581 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     5641 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     5701 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     5761 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     5821 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     5881 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     5941 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     6001 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     6061 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     6121 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     6181 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     6241 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     6301 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     6361 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     6421 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     6481 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     6541 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     6601 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     6661 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     6721 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     6781 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     6841 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     6901 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     6961 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     7021 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     7081 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     7141 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     7201 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     7261 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     7321 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     7381 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     7441 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     7501 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     7561 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     7621 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     7681 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     7741 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     7801 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     7861 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     7921 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     7981 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     8041 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     8101 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     8161 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     8221 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     8281 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     8341 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     8401 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     8461 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     8521 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     8581 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     8641 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     8701 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     8761 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     8821 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     8881 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     8941 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     9001 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     9061 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     9121 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     9181 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     9241 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     9301 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     9361 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     9421 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     9481 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     9541 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     9601 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     9661 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     9721 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     9781 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     9841 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     9901 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     9961 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
    10021 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
    10081 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
    10141 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    10201 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    10261 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    10321 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    10381 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    10441 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    10501 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    10561 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    10621 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    10681 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    10741 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    10801 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    10861 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    10921 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    10981 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    11041 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    11101 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    11161 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    11221 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    11281 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    11341 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    11401 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    11461 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    11521 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    11581 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    11641 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    11701 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    11761 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    11821 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    11881 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    11941 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    12001 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    12061 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    12121 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    12181 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    12241 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    12301 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    12361 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    12421 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    12481 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    12541 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    12601 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    12661 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    12721 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    12781 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    12841 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    12901 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    12961 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    13021 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    13081 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    13141 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    13201 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    13261 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    13321 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    13381 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    13441 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    13501 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    13561 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    13621 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    13681 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    13741 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    13801 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    13861 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    13921 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    13981 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    14041 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    14101 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    14161 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    14221 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    14281 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    14341 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    14401 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    14461 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    14521 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    14581 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    14641 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    14701 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    14761 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    14821 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    14881 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    14941 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    15001 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    15061 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    15121 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    15181 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    15241 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    15301 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    15361 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    15421 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    15481 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    15541 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    15601 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    15661 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    15721 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    15781 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    15841 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    15901 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    15961 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    16021 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    16081 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    16141 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    16201 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    16261 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    16321 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    16381 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    16441 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    16501 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    16561 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    16621 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    16681 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    16741 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    16801 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    16861 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    16921 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    16981 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    17041 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    17101 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    17161 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    17221 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    17281 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    17341 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    17401 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    17461 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    17521 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    17581 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    17641 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    17701 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    17761 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    17821 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    17881 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    17941 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    18001 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    18061 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    18121 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    18181 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    18241 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    18301 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    18361 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    18421 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    18481 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    18541 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    18601 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    18661 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    18721 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    18781 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    18841 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    18901 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    18961 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    19021 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    19081 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    19141 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    19201 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    19261 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    19321 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    19381 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    19441 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    19501 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    19561 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    19621 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    19681 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    19741 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    19801 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    19861 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    19921 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    19981 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    20041 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    20101 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    20161 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    20221 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    20281 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    20341 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    20401 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    20461 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    20521 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    20581 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    20641 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    20701 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    20761 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    20821 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    20881 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    20941 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    21001 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    21061 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    21121 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    21181 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    21241 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    21301 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    21361 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    21421 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    21481 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    21541 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    21601 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    21661 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    21721 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    21781 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    21841 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    21901 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    21961 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    22021 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    22081 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    22141 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    22201 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    22261 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    22321 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    22381 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    22441 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    22501 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    22561 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    22621 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    22681 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    22741 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    22801 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    22861 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    22921 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    22981 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    23041 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    23101 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    23161 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    23221 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    23281 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    23341 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    23401 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    23461 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    23521 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    23581 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    23641 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    23701 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    23761 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    23821 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    23881 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    23941 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    24001 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    24061 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    24121 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    24181 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    24241 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    24301 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    24361 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    24421 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    24481 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    24541 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    24601 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    24661 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    24721 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    24781 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    24841 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    24901 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    24961 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    25021 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    25081 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    25141 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    25201 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    25261 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    25321 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    25381 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    25441 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    25501 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    25561 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    25621 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    25681 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    25741 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    25801 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    25861 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    25921 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    25981 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    26041 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    26101 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    26161 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    26221 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    26281 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    26341 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    26401 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    26461 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    26521 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    26581 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    26641 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    26701 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    26761 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    26821 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    26881 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    26941 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    27001 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    27061 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    27121 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    27181 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    27241 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    27301 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    27361 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    27421 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccgtgac tttggtgaaa
    27481 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    27541 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    27601 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    27661 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    27721 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    27781 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    27841 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    27901 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    27961 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    28021 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    28081 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    28141 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    28201 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    28261 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    28321 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    28381 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    28441 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    28501 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    28561 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    28621 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    28681 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    28741 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    28801 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    28861 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    28921 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    28981 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    29041 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    29101 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    29161 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    29221 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    29281 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    29341 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    29401 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    29461 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    29521 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    29581 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    29641 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    29701 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    29761 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    29821 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    29881 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    29941 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    30001 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    30061 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    30121 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    30181 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    30241 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    30301 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    30361 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    30421 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    30481 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    30541 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    30601 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    30661 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    30721 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    30781 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    30841 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    30901 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    30961 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    31021 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    31081 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    31141 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    31201 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    31261 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    31321 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    31381 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    31441 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    31501 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    31561 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    31621 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    31681 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    31741 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    31801 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    31861 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    31921 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    31981 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    32041 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    32101 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    32161 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    32221 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    32281 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    32341 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    32401 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    32461 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    32521 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    32581 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    32641 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    32701 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    32761 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    32821 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    32881 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    32941 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    33001 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    33061 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    33121 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    33181 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    33241 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    33301 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    33361 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    33421 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    33481 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    33541 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    33601 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    33661 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    33721 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    33781 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    33841 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    33901 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    33961 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    34021 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    34081 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    34141 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    34201 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    34261 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    34321 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    34381 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    34441 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    34501 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    34561 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    34621 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    34681 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    34741 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    34801 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    34861 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    34921 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    34981 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    35041 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    35101 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    35161 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    35221 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    35281 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    35341 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    35401 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    35461 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    35521 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    35581 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    35641 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    35701 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    35761 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    35821 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    35881 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    35941 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    36001 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    36061 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    36121 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    36181 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    36241 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    36301 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    36361 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    36421 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    36481 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    36541 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    36601 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    36661 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    36721 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    36781 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    36841 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    36901 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    36961 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    37021 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    37081 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    37141 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    37201 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    37261 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    37321 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    37381 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    37441 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    37501 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    37561 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    37621 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    37681 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    37741 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    37801 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    37861 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    37921 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    37981 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    38041 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    38101 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    38161 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    38221 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    38281 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    38341 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    38401 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    38461 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    38521 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    38581 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    38641 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    38701 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    38761 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    38821 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    38881 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    38941 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    39001 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    39061 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    39121 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    39181 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    39241 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    39301 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    39361 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    39421 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    39481 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    39541 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    39601 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    39661 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    39721 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    39781 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    39841 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    39901 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    39961 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    40021 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    40081 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    40141 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    40201 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    40261 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    40321 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    40381 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    40441 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    40501 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    40561 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    40621 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    40681 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    40741 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    40801 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    40861 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    40921 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    40981 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    41041 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    41101 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    41161 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    41221 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    41281 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    41341 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    41401 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    41461 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    41521 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    41581 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    41641 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    41701 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    41761 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    41821 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    41881 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    41941 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    42001 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    42061 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    42121 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    42181 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    42241 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    42301 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    42361 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    42421 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    42481 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    42541 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    42601 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    42661 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    42721 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    42781 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    42841 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    42901 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    42961 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    43021 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    43081 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    43141 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    43201 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    43261 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    43321 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    43381 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    43441 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    43501 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    43561 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    43621 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    43681 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    43741 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    43801 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    43861 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    43921 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    43981 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    44041 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    44101 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    44161 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    44221 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    44281 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    44341 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    44401 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    44461 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    44521 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    44581 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    44641 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    44701 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    44761 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    44821 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    44881 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    44941 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    45001 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    45061 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    45121 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    45181 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    45241 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    45301 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    45361 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    45421 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    45481 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    45541 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    45601 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    45661 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    45721 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    45781 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    45841 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    45901 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    45961 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    46021 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    46081 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    46141 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    46201 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    46261 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    46321 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    46381 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    46441 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    46501 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    46561 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    46621 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    46681 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    46741 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    46801 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    46861 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    46921 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    46981 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    47041 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    47101 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    47161 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    47221 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    47281 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    47341 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    47401 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    47461 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    47521 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    47581 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    47641 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    47701 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    47761 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    47821 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    47881 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    47941 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    48001 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    48061 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    48121 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    48181 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    48241 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    48301 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    48361 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    48421 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    48481 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    48541 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    48601 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    48661 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    48721 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    48781 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    48841 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    48901 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    48961 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    49021 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    49081 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    49141 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    49201 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    49261 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    49321 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    49381 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    49441 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    49501 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    49561 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    49621 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    49681 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    49741 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    49801 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    49861 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    49921 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    49981 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    50041 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    50101 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    50161 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    50221 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    50281 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    50341 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    50401 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    50461 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    50521 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    50581 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    50641 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    50701 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    50761 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    50821 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    50881 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    50941 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    51001 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    51061 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    51121 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    51181 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    51241 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    51301 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    51361 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    51421 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    51481 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    51541 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    51601 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    51661 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    51721 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    51781 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    51841 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    51901 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    51961 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    52021 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    52081 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    52141 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    52201 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    52261 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    52321 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    52381 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    52441 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    52501 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    52561 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    52621 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    52681 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    52741 atggagcaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    52801 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    52861 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    52921 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    52981 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    53041 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    53101 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    53161 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    53221 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    53281 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    53341 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    53401 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    53461 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    53521 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    53581 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    53641 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    53701 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    53761 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    53821 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    53881 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    53941 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    54001 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    54061 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    54121 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    54181 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    54241 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    54301 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    54361 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    54421 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    54481 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    54541 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    54601 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    54661 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    54721 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    54781 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    54841 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    54901 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    54961 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    55021 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    55081 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    55141 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    55201 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    55261 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    55321 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    55381 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    55441 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    55501 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    55561 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    55621 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    55681 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    55741 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    55801 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    55861 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    55921 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    55981 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    56041 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    56101 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    56161 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    56221 gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg
    56281 caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac
    56341 ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt
    56401 gatttcaaaa ttctcgaata attctttttg attgatttta aactttttta agagatcaat
    56461 cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt ttccccattc
    56521 atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc
    56581 ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtggcaccag caactaacac
    56641 gagcatagga gaagagtact gcgcagaaag gttttcttct acgagtttca ttaaagattt
    56701 aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat
    56761 tactgctttt ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga
    56821 cgctctaata tggttattaa acttaataac ggtagatttt acatggtctt tagcatactt
    56881 atttaaaaag cgttgatatc ccactctatc taaatcagaa atcaataccg ctggcatata
    56941 tttctttatg ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc
    57001 ttctttataa agttgcatcc aatcctcaaa gtaatctgat aataatagat ttttgcgatc
    57061 ccctttaagg cctttgttca agttatattc taattcgttg gcggcatctt ttgcttctcc
    57121 ttttgtctta aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga
    57181 aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg aatcagctct
    57241 ctttctctga tacaatagga actataaaga agcccattgt ttgggtttta ttattttttg
    57301 cacatccttt ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat ggtataattg
    57361 gatgtgtatt agatcatcca atcagaggtg tttattatga ttaaagtgat ttttaaaaga
    57421 ataactgaac ttccagagtt tagattattt gttgattatt ggtatgtctg gttttttatt
    57481 gttgcattgc tgataggtct agatgtcata ttagaaagaa aacttaagta agagttgagt
    57541 taataaattg aattcctaaa tgagatattt tgtagcccat attttgtttg ccgacatcac
    57601 ctaatactat ggagctaatc gttttttcta acagaccaaa atcagataat ctatttaagc
    57661 taccatctat tagctcttgt gaatagccag acatgctgat atttccagtg aaattttcgt
    57721 ttccacgact cattaagcat gctaacaatt gaatctcaat cgatgttaat tgattcagta
    57781 caagaagata atactcctcg ttatcccatg aagcgttgtt aattcgaagt aaacaattct
    57841 tataaagatt tttatacata tctattttat tttgtgcctt tgatttttct acctcgtcat
    57901 ttatcccttc gaatattcca atgagttggt ccttgttctc taagttgaaa acatcttctg
    57961 gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat ttctcgatgc
    58021 gtttgaaacg cttctcgttt ttagacccaa aataaagtga ctgcacaacg ccaccaatat
    58081 aaggaattgc tccaataccg ccttcaacta ttaattcaag atattctttt ttggttagtt
    58141 tttcattgct attatccata gttacctcct attttgtcat ttcctttaca agcattgtga
    58201 ctcctgatgg cactcttttg tataggtttt attattgtgc acaatctaac tcttagtggg
    58261 gcgtggttca tttatttttt acttacttta ctattaatca tatcccattg agcataggat
    58321 ttttctatgt tcgatcctct tgaactaatt tctcctgtaa aagttacttt atcgcccttt
    58381 gtaaaggtat cagcattgtt taagtccttc gcaaaaatta catatgaaac tttaggtgtt
    58441 gcagaaatat cattccaact tttatcatcg tactctttgt ctgctataat ggctattcta
    58501 gtagtcattg attcgataac agttccttca aaagtatatg ttttaccact aatatattta
    58561 gtaaaagctg tattttgctc agtagctgga gtaatagaat aataactatc taagaaagca
    58621 ccccagtcat gattatttgt ctctaaaaat tcttcaaatt gagttaggct tttattgctg
    58681 ctgttagatg tagtagtttg agtatcagat gatgagcttt cctgtttgtt attgtcattt
    58741 gcctcgtttg atgaggaaag cgagaaacct acggtagtaa aaataatccc gataataagg
    58801 agaatcatag gtgttttctt atttgttttt ttgataaatc ttacaattaa catgattaaa
    58861 cctataataa ataaaacaat accagtaaaa ccaaatatac cgtccattaa tagatcctct
    58921 ttctcttttg atattgaata ttataaacca agcaattctt ttttcttaaa atcgaattct
    58981 tcttgattaa taatcccttc atctaacaac tctttatact ttttaatttc atctgcactt
    59041 gaaatttgtg tttctttgga attattatct aatatagatt gttgcttctc tatatatttt
    59101 tttaaatttt ccatttcatt ccaatatttt tttgtaaaca taacggtgtt ttcatctttc
    59161 gtggccgcta atatacctcc gcgactttcg tttccgccta gcaaggtaaa ttgtatataa
    59221 ccattactca tacctggttt ttttaattgt acggctgata tatttttaaa ggggatagtt
    59281 ttttctcctt taataccttg attaactaaa tttataaatc cttttctaat aattgaaata
    59341 gaatcatcag ttactttaat caaggttttt cctggagatt ttattaaaat ctctttttcc
    59401 attttttctt cctcatttct gtgatatgat tttttttgta gaagctcata tatgaggaaa
    59461 gagcccgtgt tgcagcacgg gcttttttaa tgttttgctg aaatcggttt ttttaataat
    59521 tcttgatgta ttcatacata ttgccttgcg taagaatatt ttttcttaaa atagcattgg
    59581 cagacagcat aacaagtgta tctgagctta ttctgaatag aataatactc cataaatttt
    59641 tcgagattaa actgagattc atcagtcagt tcgttctcaa tatagatatt taataaaatt
    59701 agaatagcta ttttatctgc ttctgtttca aattttgagt gaaaagttgt agaagtatcg
    59761 tacaaaactg aaaattcaaa atgagaggcg ctgaaatgtg caagttcatg agataaatga
    59821 aaggcttctg cagtttcacc gtatagattt tcattcaaaa aaatgattct gggttttgga
    59881 tagtagaaac caggctcttt catctccata taaactactt tcaaattgta ttcgctcaac
    59941 atttctttca atttcaaata catacaaacc atcactccaa ctattcattt tcctctaaag
    60001 ctttagcaat tgcaatcgct ttacgcattg tctccttaga tatttctttt ccatcaaaag
    60061 aaaaaacagt atcgtcttct gataaatcca catgtttagg ggtttctctt tcttctctac
    60121 ctagaaggta gtctacagag acatcgaaat agtcagcaat ccgacttaat tcatctgaat
    60181 taggagtatt atttttccac tttgctagat aaccgttaga atatccaaaa ttaatttcta
    60241 actgccgtat agatatcttt ttcctcttcg ccaagtcttt tattatttca taggtattca
    60301 tcgataaaac aacctttcta aatgtttaca aaaaaagttt agaaaaatac gcagaaataa
    60361 tttgactaat tctgagtaat gagctatact atatcttgta aacagattta acaactaaaa
    60421 agacaacaaa aaacactatt gatatgtaaa tgcagaccgc caagaaagct ttaaaatcaa
    60481 tgtttttatg tcttatttaa ttatgctctt agtatagagt attactcaga tactgtcaat
    60541 tgaatttaga aaaaagttgt taaatttgtt tacgaatata aaagaaagga atgagaaata
    60601 tgaacacacc gcaaattttt aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg
    60661 atgaaccata ttttgtaggc aaagacgttg cgagtgtttt gggttattca aacactaaag
    60721 atgctttgtc gcggcatgta gatttggaag ataagatggg gtcgcgaatc acgacctcag
    60781 gtcaatcaag agagatgaca atcatcaacg aatctggttt gtacagttta atcttaaaat
    60841 caaaacttcc ctctgccaaa aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa
    60901 ttagaaaaca tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag
    60961 atacaatcat tcaattagca actcaactaa aagaagaaag aactggaaga ttaatcgcag
    61021 aacaaaagat tgcagagtac gaacctaaaa tctcctattt agatagcata ttatcttcta
    61081 cagattcagt aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga
    61141 ataaattact tcataaacta ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca
    61201 aaaaacacat gaaccaagga tacacaaaat ctcatacaac tgagatcccg aaagccgatg
    61261 gtggcactaa aattgtaatg aataccaaat ggacacagaa agggcgtcta tttatctacg
    61321 aattactaaa aaaagaagga tattaccctc aaatggattt agaggaaatt ggttagaaag
    61381 gagttttagt atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga
    61441 atatgtcgaa agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag
    61501 caaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat
    61561 tatagattcg attatcacta tgtacgaatt gtaaagggag gaacagctaa tgcaatatct
    61621 agaagcaaaa atcccaattc cagaaggcta tgtaattatc tcccaagtgg attatgagga
    61681 gttaaaaaaa gctgatgata ctggtagatg gatgacgttg ccagaagtgc tagaacggat
    61741 taacagaaaa tatgattggt ttacttctag agttttaaag aacccaagat atagaaatat
    61801 tatcgatatc gagaaaaaca aaaatggatt tgtctattat ccagttgaag gaagagacac
    61861 atatctattt ttaagaagta aaacacttga atttttagaa acaaattttt cggaaatctt
    61921 aaggaggcaa gcggatggga aaatttaata gagcgctagt gttcagcgca ccgctaatca
    61981 tctacgcttt aggactttgg ggaagcaggc aagcgttgat aggaacgatt gtttacatgg
    62041 tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg
    62101 agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag
    62161 tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg
    62221 tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta
    62281 aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata
    62341 tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt
    62401 aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa
    62461 ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaatcata
    62521 tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat tccttttatt
    62581 tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca ttgaaaaatt
    62641 gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta taccaaaaag
    62701 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag
    62761 cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc
    62821 actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc
    62881 atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat
    62941 ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga
    63001 aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta
    63061 tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg
    63121 aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt
    63181 tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt
    63241 ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag
    63301 attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga
    63361 aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag
    63421 cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc
    63481 taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc
    63541 ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga
    63601 actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga
    63661 aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca
    63721 agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa
    63781 gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga
    63841 agagtacgac agagataact tgattgatat ctcttattac ggaccaggta agaaatacat
    63901 tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac
    63961 tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt
    64021 gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg
    64081 agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata
    64141 aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac
    64201 acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa
    64261 atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg
    64321 gactgggatt atatgtattt caaggggaag atattccaac accacctaga atcgatacaa
    64381 agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata
    64441 tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg
    64501 ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa
    64561 tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga
    64621 ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga
    64681 ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagtcat
    64741 tgaaattcgt tttattgatc ctagacgttt caccgtacag caacgaaact tcatctatgc
    64801 gctgataggc gatattttta tcgatacagg catgccaacg gacttctgga aggaattctt
    64861 ctactttcgt tttgaaggtg tcacagggcg caaaataagc ctgaaagacg aatcgaatac
    64921 aactgtgagt gatgccaatg tcttagcaaa tatcatctta gatttcatct ttgaacatca
    64981 tattcctttc aaagaaggct atgagatttt acctgcgaat caagaatatt acttctacaa
    65041 atgcatcaca aaaagagtct gctgcatctg tggcaaaaca ggagctgaca tcgatcactt
    65101 tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat catgcagagt acacatttgc
    65161 agcactctgc agaatccatc acacagagaa gcacaaaata ggtgtgatta atttcaaaaa
    65221 taaatatcaa atcaaaggga tcaaattaaa ccagaaaaca atcaaaaagt taaatattgg
    65281 agggtaaaaa tgacagaaca tcgaagttat tatgcgatta taccagccaa cgtaaggtac
    65341 gacaaaagac ttaaaccaaa tactaagttg ttatacggag agataacggc cttgtgtaat
    65401 gaaagaggct tttgttgggc aggcaatgag tactttgcag atttatatgg tgtgaataaa
    65461 gagaccatat cgcgatgggt aagtgatttg attaagtttg gatacttgaa tcgggaaatc
    65521 atttacaaag agggtaccaa tcaaataatc aataggtacc tacgaattaa tcaataccct
    65581 attgacgaaa aacgcaatac ccctattgac gaaaaagtca aagataataa tacatctatt
    65641 aataatacat ttaataatac aaaagaatat ataagagagt taccgccttc gaaaaaatcg
    65701 aaggctaagc cc
//
LOCUS       DACEOM010000005        64901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEOM010000005 DACEOM010000000
VERSION     DACEOM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.005"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            299..1960
                     /locus_tag="IW781_000285"
     CDS             299..1960
                     /locus_tag="IW781_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1181306.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IW781_000286"
     CDS             complement(2030..2377)
                     /locus_tag="IW781_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1181307.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IW781_000287"
     CDS             2461..3402
                     /locus_tag="IW781_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181308.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IW781_000288"
     CDS             3403..4446
                     /locus_tag="IW781_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181309.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IW781_000289"
     CDS             4452..5522
                     /locus_tag="IW781_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181310.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IW781_000290"
     CDS             5512..6465
                     /locus_tag="IW781_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1181311.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IW781_000291"
     CDS             6477..7487
                     /locus_tag="IW781_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1181312.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IW781_000292"
     CDS             complement(7772..8593)
                     /locus_tag="IW781_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1181313.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IW781_000293"
     CDS             complement(8690..8968)
                     /locus_tag="IW781_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181314.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IW781_000294"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IW781_000294"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1181315.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IW781_000295"
     CDS             complement(11724..12311)
                     /locus_tag="IW781_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181316.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IW781_000296"
     CDS             12486..13808
                     /locus_tag="IW781_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1181317.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IW781_000297"
     CDS             13963..14739
                     /locus_tag="IW781_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1181318.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IW781_000298"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IW781_000298"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1181319.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IW781_000299"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IW781_000299"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1181320.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IW781_000300"
     CDS             16629..17258
                     /locus_tag="IW781_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181321.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IW781_000301"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IW781_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1181322.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IW781_000302"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IW781_000302"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1181323.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IW781_000303"
     CDS             19445..19981
                     /locus_tag="IW781_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1181324.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IW781_000304"
     CDS             complement(20021..21784)
                     /locus_tag="IW781_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181325.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IW781_000305"
     CDS             complement(21787..23502)
                     /locus_tag="IW781_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181326.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IW781_000306"
     CDS             complement(24281..24505)
                     /locus_tag="IW781_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181327.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24705..27473
                     /locus_tag="IW781_000307"
     CDS             24705..27473
                     /locus_tag="IW781_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1181328.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27554..27973
                     /locus_tag="IW781_000308"
     CDS             27554..27973
                     /locus_tag="IW781_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1181329.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="IW781_000309"
     CDS             27985..28455
                     /locus_tag="IW781_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1181330.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="IW781_000310"
     CDS             28472..29251
                     /locus_tag="IW781_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1181331.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="IW781_000311"
     CDS             29238..30059
                     /locus_tag="IW781_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1181332.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="IW781_000312"
     CDS             30076..31125
                     /locus_tag="IW781_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1181333.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="IW781_000313"
     CDS             31138..32142
                     /locus_tag="IW781_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1181334.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="IW781_000314"
     CDS             32775..33794
                     /locus_tag="IW781_000314"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1181335.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33837..34718
                     /gene="galU"
                     /locus_tag="IW781_000315"
     CDS             33837..34718
                     /gene="galU"
                     /locus_tag="IW781_000315"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1181336.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35018..35395
                     /locus_tag="IW781_000316"
     CDS             35018..35395
                     /locus_tag="IW781_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181337.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            36001..36615
                     /locus_tag="IW781_000317"
     CDS             36001..36615
                     /locus_tag="IW781_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1181338.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            37388..39127
                     /gene="yfcC"
                     /locus_tag="IW781_000318"
     CDS             37388..39127
                     /gene="yfcC"
                     /locus_tag="IW781_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1181339.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            39284..40468
                     /gene="iadA"
                     /locus_tag="IW781_000319"
     CDS             39284..40468
                     /gene="iadA"
                     /locus_tag="IW781_000319"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1181340.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            40579..40782
                     /locus_tag="IW781_000320"
     CDS             40579..40782
                     /locus_tag="IW781_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1181341.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(40883..41545)
                     /locus_tag="IW781_000321"
     CDS             complement(40883..41545)
                     /locus_tag="IW781_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181342.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(41558..43060)
                     /locus_tag="IW781_000322"
     CDS             complement(41558..43060)
                     /locus_tag="IW781_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181343.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            43608..45287
                     /locus_tag="IW781_000323"
     CDS             43608..45287
                     /locus_tag="IW781_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1181344.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            45414..45893
                     /locus_tag="IW781_000324"
     CDS             45414..45893
                     /locus_tag="IW781_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1181345.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            46112..46792
                     /locus_tag="IW781_000325"
     CDS             46112..46792
                     /locus_tag="IW781_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1181346.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            46800..48131
                     /locus_tag="IW781_000326"
     CDS             46800..48131
                     /locus_tag="IW781_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1181347.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            48146..49108
                     /locus_tag="IW781_000327"
     CDS             48146..49108
                     /locus_tag="IW781_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1181348.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            49219..49557
                     /locus_tag="IW781_000328"
     CDS             49219..49557
                     /locus_tag="IW781_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1181349.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            49636..50343
                     /locus_tag="IW781_000329"
     CDS             49636..50343
                     /locus_tag="IW781_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1181350.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(50446..51927)
                     /locus_tag="IW781_000330"
     CDS             complement(50446..51927)
                     /locus_tag="IW781_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1181351.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            52236..52772
                     /locus_tag="IW781_000331"
     CDS             52236..52772
                     /locus_tag="IW781_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1181352.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            52850..54220
                     /locus_tag="IW781_000332"
     CDS             52850..54220
                     /locus_tag="IW781_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1181353.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            54287..54622
                     /locus_tag="IW781_000333"
     CDS             54287..54622
                     /locus_tag="IW781_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181354.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(54703..55560)
                     /locus_tag="IW781_000334"
     CDS             complement(54703..55560)
                     /locus_tag="IW781_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1181355.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            55667..56182
                     /gene="tadA"
                     /locus_tag="IW781_000335"
     CDS             55667..56182
                     /gene="tadA"
                     /locus_tag="IW781_000335"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1181356.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            56205..56289
                     /gene="ffs"
                     /locus_tag="IW781_000336"
     ncRNA           56205..56289
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW781_000336"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            56499..57884
                     /locus_tag="IW781_000337"
     CDS             56499..57884
                     /locus_tag="IW781_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1181357.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            57921..58598
                     /locus_tag="IW781_000338"
     CDS             57921..58598
                     /locus_tag="IW781_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1181358.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            58606..59370
                     /locus_tag="IW781_000339"
     CDS             58606..59370
                     /locus_tag="IW781_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1181359.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            59372..60031
                     /locus_tag="IW781_000340"
     CDS             59372..60031
                     /locus_tag="IW781_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1181360.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(60061..60570)
                     /locus_tag="IW781_000341"
     CDS             complement(60061..60570)
                     /locus_tag="IW781_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1181361.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(60682..61680)
                     /locus_tag="IW781_000342"
     CDS             complement(60682..61680)
                     /locus_tag="IW781_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1181362.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            61881..62465
                     /locus_tag="IW781_000343"
     CDS             61881..62465
                     /locus_tag="IW781_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1181363.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(62542..63717)
                     /locus_tag="IW781_000344"
     CDS             complement(62542..63717)
                     /locus_tag="IW781_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1181364.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            63799..64179
                     /locus_tag="IW781_000345"
     CDS             63799..64179
                     /locus_tag="IW781_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1181365.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            64253..64615
                     /locus_tag="IW781_000346"
     CDS             64253..64615
                     /locus_tag="IW781_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181366.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT        21205 a        11178 c        13370 g        19148 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgtcgt ttaggaggaa cagcaagtga
    33841 aggtcaaaaa agcagtcatt cctgccgctg ggctaggaac acgttttttg ccggcaacaa
    33901 aagccatggc aaaagaaatg cttccaatcg tggataaacc aacgatccag tttattgtgg
    33961 aagaagcatt ggattcggga atcgaagaca tcttgatcgt aacgggaaaa gaaaaaagac
    34021 ccattgaaga tcactttgat gcaaatatcg aattggaaaa caatttgcgg gaaaaggaaa
    34081 aaacagaatt actcgcttta gttgaagaaa cgactcaagt caatcttcac ttcatccgtc
    34141 aatctcatcc aagaggactg ggggatgccg tgcttcaagc aaaagcattt ataggaaatg
    34201 aaccgtttgt cgtcatgctg ggggatgatt tgatgaaaga tgatatccca ttgactcaac
    34261 aattgatgat ggattatgaa gaaacacaag cttccgctgt tgccgtcatg cgtgtaccag
    34321 aaaatgaaac atctaaatac ggaattattg atcctaaaac agatagcgaa aagggtcgtt
    34381 gcagagtaaa agggtttgta gaaaaacctg aaatgggaaa agcaccaagt aatttagcca
    34441 ttatcggacg ttatctactg actcctaaaa tatttgagat attagaaact caagaaccag
    34501 gagcaggaaa cgagatacag ctaacggatg cacttcagac tcttaaccag acagaggcgg
    34561 tctatgcgag agagtttaaa ggaaaaagat acgacgtagg cgataagtta ggatatatga
    34621 agacaaatat tgaatatgga cttcaacatt cggaaattgg gacttcatta agtgcatata
    34681 ttatccagtt gagtaaaaag ctaagaacag aaagttaact ggaaataagt ggcctgctga
    34741 gaatgaaagg ctatttgtta ttaaagcggc agcttgtgcg tcatttgcgc aactgtcgtt
    34801 ttagtttttt gactctagat cagttgagtc gtggaaaacg aaagatagaa aaagactttc
    34861 gtgaatagat tcagaaaata ttatgctaat ggtttaattt ttttttaagt tgtataaaag
    34921 aaaatgatta cataagagaa atgaaagcca agccagaaga ttcatagtag aataaaaagg
    34981 cgaatttttt aattataaaa aaaggtgtgt gttcactatg ggaaaattag tcgctgttgc
    35041 acagtcgctg cttgatatac tcaagtggct gatggaagga agcaattgga tatttgtgtt
    35101 gccactattg cttattagtc tatttatcat gtttcgcatg gtcagaaaaa aagaaacgtt
    35161 tctgattttt atctggtatg cagcatccgt tggggttgct accttgatgg ttgaattggc
    35221 gttaggtagt tgggaagctt gcgtatttgc tttatttttt gctgtactgt atctaatcat
    35281 ttatatccca tatatgaaaa gaaagtctcg gataggcaga aaaggtagac atacaactac
    35341 tttttctagt acagattctc tcgaacaaag tagaagaacg agaagaagac ggtaatattt
    35401 agtaggtata cgcaaaaaaa gataaaataa tggataaact attgagatga gagatttatt
    35461 taattggtgg aaaaaagctt ttttcactta tataaaagaa tcttttttaa aggaatgctt
    35521 gttgtatcgg gataagccat tccttttttt atgttattta tatatatata aatcatacca
    35581 atgttttttt gtattttctt gcttgcactt ggataagaga ttacttacaa taaattcaga
    35641 tagaaacagc tgaataaaaa aaggctccct gacccttagt tggcgctaag aatcagggaa
    35701 tgaccagtca cacacataca ccctgaccga tcattttgcc atagtcaatg cagtgcatcg
    35761 acctaattta ttgtacctaa tctagggtga aaattctacc cctatgtaga tattttccat
    35821 ggtctgcacg ttgttttatt tgctatgtca aggtattggg cgtattcgtt caatgccttt
    35881 tttgtgtttg aaacagcaaa gaccacatgt gttactgtct aactactgtt tttttatgta
    35941 cttccttaga ttaggcagct gtttctattc atgtcaagta tagctaggcg ggagagataa
    36001 atgagtttat gttattttga ctattatcac caattggaag agaaattaag gatagaagag
    36061 ccgctgatgc ttcaagctgg cacttggatc gcgaaacaaa ttaatcaagg tggtagattg
    36121 cagatatatg ctagccgcac actaagtggt gtagcatttg aattttggga tcaaaccccg
    36181 gatcttgtac caagtatatt gattgaaaat ccagccggag gtgtctatga aacgctagaa
    36241 ggtgcaggac aggcaattat tgatcagctt tctgtcaaat cagaagacat ttttcttctt
    36301 ctttcgaatg aaggaagaaa cccggcaatc atcgaactag ctcagtggat caaagaaaat
    36361 ggtcatccat taatcattgt tactggattt gatttgtcac gaagtatcaa atcgcaacat
    36421 agcagtggtt tgcgattatt tgaatttgct gatttggttt tggacaacca cgccaattta
    36481 catgatgcag tcttgacttt gccaaatcta gatccagcga tctgcggaac agcctctctt
    36541 gcaacgatct ttttgctgca gcaagttttg tattttacag caaaacagct agtccaggac
    36601 acaagtggaa aatagttatc cacatgttga caaagttcct taaaaaaata atgttccaca
    36661 taaaaatgga gcgtttgaaa aaaagaaaac gattattaat gaggattaac aacgactact
    36721 ttttttgttt tagttatttt aagatggatc atctttgatt ttaagcttgt tttgtttaaa
    36781 gtttgttatt tttataagtt gtttcacagt tatgagaaat gagttataag aatatgtagt
    36841 gtcagaaaaa taaaaagtag tggaaaaaga gacaagaaag aatccatatg gattctttct
    36901 tgtctcttaa tacacgttga tgaaagagtc ttccccaaaa ctctttacaa agtaattata
    36961 gtacatttgt tatcattatt gcaatgaagt ttacctgata taacaaaatt gtccaacagg
    37021 ataccttttt ctaatagaaa taaaatacac aaaaattgaa ggatagataa tggatattta
    37081 taagtgttta ttattttttt tgcaatgaaa ttcaattttt caatgattgt aaaatgaaaa
    37141 aaacaggacg agattttctc taatctaaaa ataaaaaaag aattttaagg aaaaacagag
    37201 aaataaaata cttaatataa atggaaagag aaaaaaataa tcatacatag gagaaaaaaa
    37261 acaaaataga aaaaagtgat tttctaataa attattcatg tggaaatgtc atttttactg
    37321 tataattcat ttgattatcc attttttatt catcaaaatt actgaaaaaa gaaaggaaga
    37381 aataaatttg aagaaaacaa attcttcagg aaaaccgaaa cgcgacttca ctaaattatc
    37441 gacaccccat acgtatgtca ttatttttgg tgtagtcatc tttgcatgga tactgacttt
    37501 tgtcgttcca gcaggaaagt tcagcacgca agatattgaa tacaaagatg caaatggcga
    37561 aacaagtact cgtactgttt tacgtcaaga ttccttccgt tatgcttacg agctggataa
    37621 gtcgtatgtg tttgatcaat tagaagaact gcaagatcat ccagcagaac gtgaaaaact
    37681 ggatgttccg gaaaaaggat tggaaaaagt cattgctgac ggagaaaaaa atcttacgca
    37741 ggaaaaacta gatgaaatct ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa
    37801 tatctatgat acttctaaaa aattgcataa aacagccaaa atatggggaa cagatgattt
    37861 tggcggcttt ggtttcttaa attttgtttt tgaaggacta gtatctggag ataagtatgg
    37921 atctgctgta gggatcgctg cattgatcct tgttgttgga ggtgcttttg gtattattat
    37981 gcgtaccgga gcgatcgacg caggtatcta tgcgtttatc agtaaaacta agggactgga
    38041 gcgtttagct ttaccgctat tattctttgc tttttcattc ggtggcgcta cattcgggat
    38101 ggcagaagag gtaattccat tctcaatggt gatggttcca tttgtcattg cgttaggata
    38161 tgactcgatc gtagcagtaa ccgtgactta tgtcgcatct caagtcggaa atgcgacctc
    38221 ttggatgagc ccgtttagcg tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc
    38281 tggtgcgaca ttccgattga ttatgtgggt agttgttact gcattagccg ctggttatat
    38341 gatgatatat gcagaaaaaa tccgtaaaaa accagaaagt tcgttgactt acaaatcaga
    38401 tgattatttc cgttctcata tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg
    38461 gcataaattg atcttattag agatgttagt tgtccttgtc tggatcgttt ggggcgtgac
    38521 tcaaaaaggc tattacattc cagaaatcgc ttctcaattc tttgtgatgg gattagttgc
    38581 tggaatcatt gctgttcttt tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc
    38641 tttccaatct ggtgcagctg atttagctgg tacagcaatc gtagtaggta tggccaaagg
    38701 gattcttctt gtcttgggag gatctgatgc atctgttcct tcagctttga atacgatttt
    38761 gcatgggatc gggacggcgt tgaccggtgt tccagctgtc attggtgctt gggcgatgta
    38821 catcttccaa agtttgttca acttggttgt tacatcaaat tcaggacaag cagctttgac
    38881 tatgccaatc atggctccac tggcagattt agtaggtgta tccagacaag tagcagttct
    38941 tgcttatcag cttggttcag gttttatgga cgcttttaca cccgtatcag ccagcttgat
    39001 tggtgtgcta ggtgttgcac ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat
    39061 gcaaggattc ctgtttgttt taggaactat tttcatcatg attgccattg caataggtct
    39121 gcagtagtac tggagacctg taaataaaaa agtaatccaa acagtagata aggactccaa
    39181 gaaccgtcat tttacggtaa aatcaaagga gtaaagatcc tatcgtttgg attatttttt
    39241 aagatcctat catcagttat acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca
    39301 aattgaagtg tttgcaccag attacttagg aaaaatggat gtactgattg ccggtgggaa
    39361 gattttggct gtagaagaac agctagaagg cggctacgaa ggagtggaag tagaagaact
    39421 ttcaggtgag ggaaaagtac taaccccggg tttcattgac tgtcatttcc atattttagg
    39481 tggaggagga gaaggcggtt atcaaaatcg aacgccagaa gtcacattga gccaattgac
    39541 tacagctggt gtaacgacag ttgtcggttg tttgggaaca gacggagaag gtagagatat
    39601 gactgcgttg atcagcaaag cgaaaggtct ggaagcagaa ggaatctcca cttatgtata
    39661 cgaaggttcg taccgattgc cggtaaaaac agtaacagat tctatcatca aagacttttt
    39721 gacaattgat aaaattatcg gaatcggaga aatcgcagta tccgatcacc gttcttctca
    39781 gccaagtttt gaagagttcg ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc
    39841 cggaaaagct gggatcatca atgtccactt aggcggagga aaaagaaagc tagagctatt
    39901 gacacgaatc gtagaagaaa cagaaattcc gatcacacaa tttttgccta ctcatgcaaa
    39961 tcgcacacca gaattatttg aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga
    40021 cttcactgct agcgaagatc ctgatttttg ggagaagaca gatggtgaag ttcgcttcag
    40081 caaagcactg aaacgattga tcgaggaaga tgtttcttta gataatttca cgatgacctc
    40141 agatggacaa ggaagcttgc cttattttga tgaaaacaat catttcctag ggttaggagt
    40201 aggaagtgcc aaggcattgc tagtagggat caaagaagct gtgcaaaaag aaagtatccc
    40261 tttggaaatc gccttacgag ctataaccag taatccagca cgtatattga aattggataa
    40321 aaaaggaaaa atcgaaattg gtgctgatgc agatctgtgt atcttggata aagaaacgct
    40381 tgatattgat acagttatcg ctaaaggaga aataatggtc caagaaaaag aagtcaaagt
    40441 atgggggact ttcgaaaaaa gtttttaatc aacagctcta actatctttt ttagaaaacg
    40501 agcgttctgt ttgtctgtat ctgttgttga tgttaccctg aaggtagatc aataaacaaa
    40561 ctaagaggag gaagcacaat ggcagattta aaaggacgtt ctaaagatat gaaagataaa
    40621 gtagtcggtg aaggaaaaga agcatacggc aaagcaacag acgacaagag caaagaaaca
    40681 gaagggaaag cacaatcact aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac
    40741 gaagtcaaag acaagttttc tcgaaaagac gaaaaaaaat aaaaaccact tattactaat
    40801 tgaatcaagc aaataaaaaa tccgaaccat atctgcgaga cagatatggt tcggattttt
    40861 tatttgatca ttatcctgtt ttttaaactt cgattttttg aaatttttta ttttttagtt
    40921 caaagacttc atcacagctt tttgctactt caatctcatg agtcacgatg ataatacagc
    40981 gattttgttc atgggcgatt tcttggaaaa gtttgacgat atcttgtgaa gttgtttcat
    41041 ctagatttcc agtaggctca tccgctacga ttagttgatg ttcacagcag atagcacgaa
    41101 cgatagcgac tcgctgctgc tgacctcccg aaagtttggt cacctgtttt tttgctaata
    41161 tttcatcaat tcctacacga gccagtcgat ctaaagcaac ttgtcgataa ttctcttgct
    41221 tggtcttagt gatttccatt gctgctatga cgttgtctag agccgataaa taaggaagca
    41281 agttgtaagc ttggaaaata atcgagacat catttttacg ataacttctt aacccgattt
    41341 tgttcagtga acgatcttga tagtatacct ttccttcttt tggtgcatct agtccagaaa
    41401 taagcgaaag aaaagtcgtt tttccagaac cacttgctcc taaaatagcg tacatttttc
    41461 cagcttcgaa attcagttct acatctttat aaagcgcatc ttgcggatct tgataccaaa
    41521 atcccaattg ttctgtccgt aacatcaaat tcctccttta gtttaatagg atttttcttg
    41581 ggtttaatct taatatccct atagaagcta aaaagacaga aaacagactg attccaaatg
    41641 ctactccggc taatagtccc aattgtgcag gtttgacatt gatttccaat tcgctgactt
    41701 gatcagaaac agcaaatgga ttattcgtgg ttccttgttg accatcgcgt tgaccgcctt
    41761 gttcttgtct gttacctggc atttgtccac cgccaggagc ttgctggttt tgtgtcgtgg
    41821 tttctgtctg ttggtcaagt agctgctgtc ccacggcatt cgcgacgacg tttccgctaa
    41881 gcgaagcaac tcctaatgca agaatcatac aaatagccac ttcggtaaac aattggagaa
    41941 tgattttact tctggattct cctaaagata gcaagacgcc taactcatgc cgacgctccc
    42001 gaatggtaat cataatgatc agagtaagta tgataatacc ggctacagcc actagtaaaa
    42061 caacattttt agaaaaactt gccacgttgt ttaaaggttc caacattgac tgatacatgg
    42121 aatcatttga ttgtaagcta aatgtatctg tgtcgatcag cttctcagct tctttgagaa
    42181 actcatccat tttatctgga tcagatagag tgtaaatagc agaatctatc gtgttatcgt
    42241 cagatgtccc atttagttca tttgcaaaag tgtaagaagt gtaaagtgta ttcgacggat
    42301 tcatgaagtt gaatttcata cccatactgc cgcttgtttc gctggattca taaattcctt
    42361 tgatcgtcat ttcataagtg gtatcttcat cttcagtact cgtgattgtg aaagtatctc
    42421 cgactgccag gtcatttgtt tctgctagag tagaatcaat gactacattg tttgtgcctt
    42481 tgtcgctgtc atctattcct tcaccatccg tgatttttgc tgttccatca gaaaaattac
    42541 tattttgtgc agttgctgac acaccactga tttgaaagtc tgcttgagtc atttgcggat
    42601 tcccgcccat tcctccagca ggttcaccgc ctgcatcagt agtttctgaa ctgtcttcac
    42661 ttgaatcaga actagtgatg gcagttattc catcttttgc catagctgag gcggaacttt
    42721 caaaggaata actctttaca ttatctaatt cagctatttt ttccgcgtcc gttaaggaaa
    42781 ctggtgtaag agaaaaactg cctggatctg gacgttcttc agtggtagaa ccgcttgaag
    42841 aagtttgttc ctgtttttta aatgaagctt ctcgattggc acttaaagtc actgttgctc
    42901 caacttcttt ttgtgcttga ttcgctgctt cttctgcagc gcttcgaatc gtgaggccag
    42961 ctagcacaaa aataagaatg gctgtgaaca cggcaaacaa taacaatgaa cgtccccatt
    43021 ttacttttgt acttttccat gcacgtttca taaaattcat attttgcatc cctcctagta
    43081 gatgcatttg ataatagaca acctatctta aagctagctt aaatcttatg ctgtttgcac
    43141 accatcaaca tgaggttctg tatatataaa aagtcactat agttattgca aaaaatgtgg
    43201 taaaatgaaa aaagtatgtt tcaaaccgac gaagaagaaa tgaaagagga atagcaaaag
    43261 aaatcctaga tgctacagag ggatttttac gtgcatagtt gttctaacaa ttgctgatac
    43321 tagcgattta gtttatttgt tgtataataa tggggaacag ctgaatactt atataagtat
    43381 tctggctata gcgtttcgca aaagatccat tttgttaatg aagtatatcc aaaataaaat
    43441 gcccatccaa ctgtcagaac gcggatgtgc tggtaatttt cctgctgatt ctccataagt
    43501 tgagaaatag tgattttgca tatgcttcat tggaatggct catatattta gataaagaag
    43561 aaaatcgaca tctttaaact tggatgtcat aagaagaggt gaagtttgtg agtaccatta
    43621 aaattatccc attaggcggc gttcgtgaaa atggaaaaaa catgtatgtc gcagaagtgg
    43681 gcgaggatat ttttatttta gattgtgggc taaaatatcc tgaaaatgag ttgttgggga
    43741 tcgacgtcgt aatccctgat tttacgtatt tagaagaaaa cattgatcgt gtagctggtg
    43801 tctttttaac acatggacat gcagatgcga tcggggcact tccttacttg ttgtcaaaaa
    43861 tttctgtacc agtattcggg acagagttga cgatcgagtt agcaaaattg aatgtcagtc
    43921 gtaatgaagc agccaaagga tttaaggatt tccatgtggt agatgagcat acagaaatag
    43981 actttggtga aacagtagtg agctttttcc gtaccactca tacgatacca gattcagtgg
    44041 ggatcaatct gaaaacaaaa gaaggtagta tcgtttatac gggagatttt aaatttgacc
    44101 aaacggctat cccaatgtat caaacagatt atgcacgatt ggcggaaatc ggcaaagaag
    44161 gagtcttagc cttactaagt gaatcggcaa atgcagaaaa tcctcagcct gtagcttctg
    44221 aagcccaaat agcagatgaa gtatttgata cgatcaaata ttgggaaggg cgtatcatcg
    44281 tcgcttgtgt agcaagtaat ttgcaacgag tgcaacaagt attggatgca gcagaaaaag
    44341 cacaacgaaa agttgtcttg actggtcaag atttcgaacg aattattcgc acagcgatgc
    44401 gtttagaaaa acttcaatta ccaagtgaag atctgcttgt tacgttgaaa gacatgaaaa
    44461 actatgatcc agaagaactg atcattttag aaactggccg aatgggtgaa cccatcaagt
    44521 ctctgcaaaa aatggcaaac ggtacacacc gtaatgtcaa gattgaagaa ggagatttgg
    44581 tttatatcac tactacgcca agcatcgcga tggaaaccat cgtggcgaaa acggaagata
    44641 tcatttatcg agctggtgga acagtgaagt tgatctctga gaatatgcgc gtttctggcc
    44701 atgccaatcc taatgacttg caattaatga tcaatttgat gaaaccaaca tattttgtgc
    44761 cagttcaagg agagtatcgt gaattagctg ctcatgcaga tttggctcat gcagtaggga
    44821 tgccatacaa aaatatctat attactggac gcggagacgt gctggaatat aagaacaagc
    44881 gtatgagtgt tgcaggaact acaagtgcag aaaatgtaat gattgatgga ttaggcatag
    44941 gtgatatcgg aaacattgtt ttacgtgatc gaaaaatctt gtcagaagac gggattttcg
    45001 tagcggttgt cactatcagt cggcgagaaa agaaaatcat ttcaaaacct caaattactt
    45061 cacggggatt cgtttacgta aaagctagcc gtgatctgat gaaagaaagc agcaatatga
    45121 ttgaaacaat cgttgagaaa catttagaaa gcaatgattt tgagtggagc aaattaaagc
    45181 aagacatccg agatcaatta ggccgttact tatttgaaca aacaaaacgc cgcccagtga
    45241 tcttgccagt cattatggaa gctactcaac gtcgaaataa aaaataaaca aaaagagtaa
    45301 gatgaacctc tctatagagt gctcgtcttg ctcttttttt gcgaaaaaca aataattttt
    45361 gaatacagca tccctcgctt tataataaac gatagaataa agggagtgaa tcaatgatga
    45421 gtcaaacaaa agtcgaacgt tatttagaag aacaaaagat cagctatcaa ccacttgatt
    45481 ttagtcatgt ggtagaagaa tctttcacag aagaactaca aaaaagaggg attgatccag
    45541 ctttgatatg taaaactctc gtagtgaaag gaaacaaaac tggggtgatc atcgcattga
    45601 ttcctttgaa cgatcactta gattacaaaa aaacgagaca agtgacgaaa gatcgtaaag
    45661 ttggttttcc tggaatggat tttgtattaa tgcatacagg atatccccac ggagcaaaca
    45721 cccctattgg aataaaactc gcacatcctg attattgttt tttagcagac agttctttaa
    45781 aagaaaaaga agaagtaatt gtgtcaagcg gtgaaatcgg gagaagcctt cggataaaag
    45841 tagccgattt agaaaagatc gtccatccta tttacgctga tttaattagt taagggacga
    45901 gaagagaagc taagcagagc agagacctca aacttgagat tatatctgct tggtacttgg
    45961 tgcatcctgt attatctgtt acaattgctt tgtacatact gtactatcaa tccaatgaaa
    46021 atatatttag cagctatatt ttttcattga tgtgcaagtg atgaaatcgt ttttaataaa
    46081 aaagatacag agaaaacgcg aggtgtaaaa tatgaaaatt acgtatcatg gacattcctg
    46141 tttaacaatt gaaatggaaa atggccaacg cgtgatgatt gacccgttta ttacaggaaa
    46201 tccattgaca gacttaactg cagaagaagt aaagacagat tggttgctga ttactcatgg
    46261 tcatagtgac cacataggag atatgattcc tattgcaaaa gaaaatcaag ccacaattat
    46321 cagtattgtt gagattgctg attatgcgaa cagcagagga gtagattcat tcggtatgaa
    46381 tatcggtgga aaacatgcgt ttccattcgg cgtggtaaaa ttcgtacatg cacagcatag
    46441 ttcaagctat gaagtagacg gtatcgttca gtatatgggc gagcctagcg gaattattat
    46501 tcaagcagaa ggaaaaacga tttatcatgc aggggatact gcttatttca gtgatctagg
    46561 attgcttgca gaagaattcg atattgatgt cgctttcttg ccgattggcg ataactatac
    46621 gatggggcca gaagatgcag caaaggctgc gcaaacaatt cgtgccaaga aagtagttcc
    46681 aatccattac aatactttcc ctgtcattga acaagatccc gaatcttttg tagagttact
    46741 ggaagatagt gaaggaaaaa taatgagcgt ctcggaaacg ctggaagtgt aggcagaaaa
    46801 tggcgacaaa acatgatttg attttagaat atattgaaag tttacctgtt ggcaatcgaa
    46861 tttctgtaag aagcatcgca aaagaattga atgtcagtga aggaacagcg taccgtgcaa
    46921 tcaaagatgc agaaaatgtc ggactagttt caacaattca acgagtaggg accatccgta
    46981 tcgaacgcaa attaaaaaaa catattgaac gtttgacatt tggcgaggtc gtcaggatca
    47041 ttgaagggga cgttttaggc ggggcggcag gattagacaa ggtcttgaat aaattcgtca
    47101 tcggcgcgat gactaaacat gccatggaac gctatattac gccaggatca ctaatgatcg
    47161 tcggcaaccg ggcggaagtc caaaaattag cattggaaga tggcgccgca gtattgatca
    47221 caggaggatt caatacgact gctgagattg caaagctagc tgatgatctt gaaatgccga
    47281 tcttacgcac ctcttatgat acatttacag tcgcaaccat gatcaaccga gcgttaagcg
    47341 accaattgat caaaaaagat atcatgcttg tcagtgatat ctatatgcca gctgaaaaga
    47401 ctcattattt gcagcagaca gatacgattt ctgattatca gcgtttgtca gaagaaacgc
    47461 agcattcgcg ttttccagtc gtcaaccgcc atcatcgttt gatggggatt gttacggcaa
    47521 aagatgtttt agggaaatcg ccaaaccaga tcatcgatcg ggtgatgacg aaagaaccaa
    47581 tcagtgtaaa gaaaacaatg agtattgcct ctgttagcca tcaaatgatc tgggatgggc
    47641 tagaagtcat gccggtcgtt tccgatgact tgacattgga aggcttgatt acccggcaag
    47701 atgtaatgaa agcgatgcag cttgtccagc gacaaccgca gattgcagat acgatttcgg
    47761 atcagatatc tggaaatatc catacgattg atactgaccg tgaaggaaat cgtttggaaa
    47821 accctaagtt caaattcgta gtagcaccac aaatggtcaa tggcgttgga accatttctt
    47881 ttggcgtact tagcgaaatc atttctgatg tcgcacaaaa aacaatggtg atgaaccaaa
    47941 aacgaaatat cttgattgaa caagtgaacc tacattattt gcggctgatc caattggaaa
    48001 gcgaattaga cattcgtcca cgtgtattgg aaatcggcag acgttccgct aaactggata
    48061 tcgaagtgtt tattgaaaac accatcgttt ccaaagcaat cgttgtttgt caagtaatgg
    48121 aacgttctta gaaagcagga gtgaaatgac agtacagcaa gaaatagtag atacaataaa
    48181 aaaatacgat aaaatcatta tccacaggca tttgcgccct gatcctgatg cattaggatc
    48241 acaagtagga ttagccgaac tattacggga aagtttccca gaaaaagaaa tacgtcaagt
    48301 aggtgaaacg attgaagggc ttcgcttttt agcagaaatg caagaagttg aagatgtctt
    48361 ctaccaagga gcgctagtca tcgtgactga tacagctaat tcacccagaa tcagtgatca
    48421 aagatatcag ttggcagaaa aactgatcaa aatcgaccat catcccaatg atgaccctta
    48481 tggagatctt gtgtgggtaa atacaaaagc aagtagctgt agtgagatga ttgcagaatt
    48541 tgctttgatg tttcctgaat ctttaaagat gaacgttaag gctgcacggt tactttatgc
    48601 aggaatcgtc ggagatacag ggcgcttttt gtatccctcc acgacgtccc gtacattaga
    48661 gattgcttcg atattgagaa attatccatt tgatgcaagt gctttgaaca gagagattga
    48721 acaaatgccg atgaaagtcg ctaaactttc cggttatctt tatcaaaata tccaggtgga
    48781 tgaaaatggt gcaggaagaa taatccttcc gcaagaaatt cttgataaat atggaatcga
    48841 tgattcagag acagcttcga tcgtttcact tcctggagtg attgacgaag tgctggcttg
    48901 ggggattttt gttcagcagc cttcagggta ttaccgcgta cgtttacgtt ctaaggggcc
    48961 gatcatcaat gaattggcta aacgccatca tggaggaggc catccattag caagtggtgc
    49021 gaatgcaaaa gatttagcag aagtagagga aatctataaa gagatccaag atatatgcca
    49081 atcgtataag agcagaaaaa cagaatgata cttcgttcga aaacgtgcct tttgtagtaa
    49141 aaatcacaaa tgtatctttt caagatcttc tttttccgtt acaatagtgc tcactaaaga
    49201 ttttaaagga gaaagaagat gacattacca aattgcccag aatgtggctc agagtacaca
    49261 tatgaagatc gaggcttgtt gatttgccca gaatgcggga acgaatggaa caaagaagaa
    49321 caagaaactg tcatagaaga cggattagtg gtattagatg caaatggaaa ccgtctgtta
    49381 gagggcgata gcgtgacagt gattaaagac ttgaaagtaa aaggtgcgtc aggtgccatt
    49441 aaacaaggaa caaaagtcaa gaacatccgt ttagtagaag gcgatcataa tatcgattgc
    49501 aaggtagaag gatttggacc gatgaaactg aaatcggaat ttgtgaaaaa gaattaaaga
    49561 atattcaagg tcttttcaca ttcttttcct actactaatg ataaaataaa gtaaacgaaa
    49621 aggagtgaaa cgaaaatgta tggttatgga atgtatggtt ttttagatcc gacatttctt
    49681 ttggtcattc ttggattagt tatttccgga attgcgtcag cgtacgtcaa tagtactttc
    49741 cgaaaatacg atcaagtgag aagcaaaaac aatgtgactg gtacacaagc agcacaatat
    49801 atattacaaa gtcaacagat aaataatgtc ggtgttcaac aaattgccgg agatctcaca
    49861 gataactaca attcaggtaa taagatgttg agtttgtctc aagcaactgc acagtctact
    49921 tcagttgctg cgataggtgt agcagctcat gaatgcggac atgcggtaca agatgcttcg
    49981 ggttatgtcc cattgaagat tcgtgcagca cttgttccag cagctaactt tggttcgatg
    50041 atttcgttcc ccttgattat ggtaggtgta ttgcttagct ggaactctac gttaatcaat
    50101 atcgggattt tcgctttttc tttagcatta cttttccaat tggtgacatt accagtcgaa
    50161 tttaacgcgt ctcggcgagc aatccaaatt ttgagtgaag gcggactact gacggaagaa
    50221 gaagtaccga tggcaagaca cgtattattt gcagcagcac tgacttatgt tgcagcagcg
    50281 ctttcgacat tcctgcagct gttgcgcttg atcttattgt tcggcggcaa ccgaagagat
    50341 taaaataaag aagttgtgaa atcagcgctt tgatctgagc taataccgag agttagctga
    50401 ttgaatacca tttatttgga ataagaggat gtgacaagaa ttttgtcaca tcctctttgt
    50461 tttgttctca taaccttttt tcatatacag ttcatgaacc gcttttatta acttgccaaa
    50521 taacactgta ttgtgcgttg tatccagata gaaagtctcc gtttcatctg aaattttctc
    50581 atgctgatct gtaatgatca aatcagcatg ttctgaatat gcggtaattt taacagcatc
    50641 tttgcgaaat aactgctgca aatggctttg aagatgataa tttcctgtaa actgtttggt
    50701 tatctgcaaa tggatgagta actgctcttt cgtttctgtt tgtagtaagt gttggatcaa
    50761 ttgactaagg tattgactga tttggtttct ttccccattt tttgaaaaaa ttgtatcgat
    50821 tttttctctt aatttctttt cctgtataaa agaactagaa tgattggacg tggcttctgg
    50881 aaagcaaagt gtagaaaaac tactgctgga ttcacccaac agttcgaaaa aaacaactgc
    50941 caacacaaga aaataaaaat aaattggtat tttattttct aaatggttga gaggaaactg
    51001 ttgagttaag taagtcatca attgaccgac ttgtcgacaa ataaggtttt tgcttgtgga
    51061 aaataaaaaa ccaatctcct gtttttgtcg aaaagagatt tgatttggaa aaaaacaata
    51121 gaaaaaataa ttaaagtagg ctctctctgt ttctgtctct tcacaagggc gactcactga
    51181 aaaagaagta aagtattctg aataatcatg tgtatgctgc aataagtcca tcaattcagt
    51241 gatttcagaa gaagtttttg gaaaatatgc ctgttgtttg tttcgtaata tgaataaagt
    51301 taacagcaag tagatatttt tttcttgttt tgaaattttt tctatttgct catctgtact
    51361 gttcattttt agtgcattta attcaagtga ggaaaatggc caatcgttac ctccaaatgt
    51421 atctgaaaga aaaaagtaaa gatagagacg gatagataac tcatttcctt caagagtaca
    51481 gcaatttttt acagatagga tccgtaaatg gaattttttt aaggaatgat tgagctgttt
    51541 tgtcagtttt tcgagatagg agggcgatat ataaagttgt tcaagtaaat atttttttgg
    51601 tacagcagtt tgtggataat tgattagtaa catcaaatag ttaaaacgat tggattcttt
    51661 taggtagcat aattttaact tagaaaaaac agctaacttt ttagtttgat cggtttcttt
    51721 ggtttggatc tctcctttat gattagaata aataaattca tcaatcaata aaaattgctt
    51781 gagtgataca tttatttttc ggatcagttc tttgattgtc cgttttggaa tatgtaagtc
    51841 attggataat tcttgtaaag aacaattatt tttttgaatg accatttgta gaacaagtaa
    51901 ctcttttttt cttgcttcaa ttagcataaa tacctccttt ttattctgtt tttgtatata
    51961 gttagacaaa aactgtgagt ggaaagtcat ttcagtctgt gttattctca cagagtgtcg
    52021 ccttcttttt tatataacgt tatttaattt ttttgttacc ttgtttattc tattgtttat
    52081 ttcctagggt attttgtcaa gatattcttg tttttaaggc ggaaaaattg atttttaatg
    52141 gatataacta aatatgtttt gataattatc aataatattt ttagaattat ttatgttatt
    52201 tatttatggg tagatgaaaa gagacggagg taagaatgat ggggagagta acaaattata
    52261 gtaaagaata tttgctgttc aaaagtatgg tatacgtgga agaatatgga atgaagtcaa
    52321 ttactgcaag agagttagca gagttctgcg gttgttctac atacccgatt tatacacatt
    52381 tcaaatcaat cagcgggtta aaagagaaga tattagaaga aatcactgtt tattttgaaa
    52441 actacttagc tgaatgcagt tccgatgatg ttttaagcat tgtctactta ttgaaagatt
    52501 ttttttctat ccatgaaagt attcgtgaaa tcattgcaaa atctgattta gacatggatt
    52561 cgctatttaa gcggccattt ttctcatatg tcaaaggtca cacgaagatc aaagaagaaa
    52621 agtcgcttga attggtatgg cttaatgcat tgggaagttt gcgctcagat gattcttctc
    52681 aagattttct tgaaatgatg aacgctgttt gggataaaaa cgaggtgacg gatgaatact
    52741 tggaaaatat cttaaaggaa cagtcacctt aggcagcaac atgaaaaagt agatcagcca
    52801 cctttatttt tcgtaaaaaa gattgtaaaa tgtctaacga aacattggaa tgaaggagga
    52861 agcgacaacg ttaccacagc aatttatcaa aaaatatcgt cttcttttag gcgaagaagc
    52921 atccgatttt ttttctgcgc ttgaacaagg aagcgtgaaa aaaggatttc ggtggaatcc
    52981 actaaaaccg gcaggattgg acatggtcca aacttaccat agcgaagaat tacaacccgc
    53041 tccctacagc aatgagggat ttcttggcac tgtcaatgga aagtctttcc tccatcaagc
    53101 tgggtatgaa tatagccaag aaccaagtgc aatgatcgtt ggaacagctg ctgcagcaaa
    53161 acccggtgaa aaagttttgg atttgtgtgc ggctcccggt ggtaaatcaa cccaattagc
    53221 tgctcaaatg aaggggaaag ggttattagt gacgaatgag atctttccga aacgtgcgaa
    53281 gattttatca gaaaatatag aacgatgggg agtttcgaac gcaatagtga caaatcatgc
    53341 acccgcagaa cttgtccctc atttttcggg cttttttgat cggatcgtgg ttgatgctcc
    53401 ttgttcagga gaaggaatgt tccgaaaaga tccaaatgca ataaaagagt ggacagaaga
    53461 atctccgcta tactgtcaaa aacgtcagca agagattttg tcttcagcaa taaaaatgct
    53521 aaaaaataaa ggacaactga tttattcgac ttgtacgttt gcaccagaag aaaatgaaga
    53581 gatcattagc tggcttgtag aaaattatcc agtaacgatt gaggagattc ctttgactca
    53641 atccgttagt tcaggacgtt ctgaatgggg aagcgtagct ggtttggaaa aaacaatacg
    53701 tatatggcct cataaagatc agggtgaagg acattttgtt gcaaagctga cttttcatgg
    53761 acaaaatcag atgcataaag aaaagaagac acgaaaaaaa agtaaagtgc aaatgacaaa
    53821 agagcaggaa aaactctgga cggagttttc aaatgatttt cattatgaag ctactggaag
    53881 acttttagtg ttcaacgacc atctttggga agttcctgag ttagcccctt ctctcgatgg
    53941 tttaaaagtc gtacgtacag gtttgcattt aggagatttc aaaaagaatc gatttgaacc
    54001 tagctatgca ttagcgcttg ctacaaaaaa gatagagaat ataccttgct tgccgatcac
    54061 tcaaaaggaa tggcaatctt acacagcagg agaaacattt caacgtgatg ggaatcaagg
    54121 atgggttttg ctagtattag ataagatacc tgtcggattt gggaaacaag taaaaggaac
    54181 agtaaagaac tttttcccta aaggattgag atttcactga gtggattagt tcgtttattt
    54241 ttttgtctat gctatgatgg agatagataa aaaggagctg atacttatga aaacatcaac
    54301 aaaagtaaca atcggattaa gcgtagcagc cgctgccagt gtagcgacag cagtcgttgt
    54361 atcgggaaaa gtaatggaaa agatccatca tatgactact cggacgaaag taaaaaaatt
    54421 cgtctatgac aaatttgatg gaaatgaaaa attactagaa atcgtagatg gtctttcaga
    54481 tagtgacttg gattctttga tgggaatgtt agcaaaaatc aaatcaggaa aaaagaaaat
    54541 ctctgtttat ggtgattcaa tcaaagattc aaccgaagat gtaaaagacc ggctaatgca
    54601 ttttgttgaa aaaatgatgt aagagaaact agcttttgaa cactattcat tcgaaataag
    54661 gggttgtgac aaaattattg tcacaacccc tttaatctat ctttaattat gttcatgaag
    54721 caataaaaga tagcaaaaca gtaaatcgtt ggcttttttt agattgaaac cagactgttc
    54781 ttgaaatttt tcgattcgat attttagagt attacgatga agaaacagtg tttttgcagc
    54841 agagctgaca ttgcctaatt cattccataa ttcttgaatg atcacttcca tttcattttg
    54901 tttgatcagt tctatgtaac ttttgatcaa cttactatct tttacagtat cttttgtata
    54961 gtaatgtaaa gccacatctt gcatagaaaa agtacggctg tgagtataaa gattgttttg
    55021 ctctgttaaa aaaatttttc tttcttcagc aaacaaaggg acgaggtcgg tgccaggtga
    55081 atggaaagaa ccaacgaata cagacgttgt cgtatcaaaa tcagcatcta aagatagaaa
    55141 aataccgttg atttcagtag catcaagata atcacggttt atttgttcga ctaagagcgc
    55201 atctgtgtct gtataaaaaa agaaatcagc agaatagcga aataattcca aaatagtatc
    55261 ttgccattct ttttttaaga attctccttt tgattcgacg tgaagctgga tcaaacgaaa
    55321 gcttttttct tgaacacaag aagcatcttt aaaaagatag tgataccatg ggtgtttttt
    55381 ttgatcgttg attacaggga ataacgcttt caaaaggctg gtttcttgtt gagataatga
    55441 atcttgtttg atccagataa aagcatcatc aattgcaaaa gaaaggatat ccgcttctga
    55501 tgctggagag ttttttttct gtgcttgtgg gtaaagagca agtaatttat ccattttcat
    55561 cttgttcacc tcttgtattt attttaccat agaaaaaaaa gttagttaca gcctttttat
    55621 catatgctaa agtaaaacaa gaaaaaacaa aggcagggaa gggtaattgg taaatacaaa
    55681 tcttgatgca gtagaaaaag aaaaatggat gcgccttgca ttggcagaag caaagaaagc
    55741 agaaatgctt catgaagttc cgattggagc agtggttgta ttggatggaa aagtgattgg
    55801 aagaggatat aaccttagag aaaccacgca agatgctact acacatgcag aaatgcttgc
    55861 aatcaaggaa gcctgtgaaa aagtgggaag ctggcgcttg gaagatgctg cactttttgt
    55921 taccttagag ccttgtccta tgtgcagcgg cgcgatgatt ttgtctcgtg tagcagaagt
    55981 ttatttcggc gcgtatgatc caaaaggcgg aacagccggt accttgatga atcttttgga
    56041 ggacgagcgg tttaaccacc aagcatatgt agaaggtggc attttagaag acgaatgtgg
    56101 gatgattttg actgactttt tccgaaagct ccgccagcga aaaaagaaat taaaaaaaga
    56161 ctggtcaacg aatgaaaaat aaggtatact attctttgcc gaaaggctag gacaatggtg
    56221 ggcttgtgaa gcgtgtcagg tctggaagga agcagcacta agcaggttcc gccatgtgtc
    56281 tgattataat gaaacgacaa cagactttcg ggtctgtttt ttttattttt atagtactgt
    56341 aaaaatgaat gttcctgatt ttttagccag actgatttat cgaatattgt acttatacaa
    56401 tttgcttcaa ggcaggaaaa actctgaaat agagcttgtt tcaatggaag aaaaagctgc
    56461 ctatggtatg ataaagataa agagaggcga gagggtacat ggaaaaaata gtgattttac
    56521 atacgaatga tctgcattct catttagaaa attggccaag gatcagaagg tttttggatc
    56581 aacgtaaacg agaaaacgaa aagaaagaaa aaacgacaac gattgcagta gatctaggtg
    56641 attttgtgga tcgttggcat ccattgtcag aagcaacgaa tggacaggaa aatatcttgc
    56701 tgatgaatga agttggttat gatgcagcga cgataggaaa caatgaaggc gtaggaaatt
    56761 ccaaagaaga gttgaatcat ttatacgatc atgctgagtt tgatatcatc ctggataact
    56821 tattcgataa aaatacgctc cagccaccgg catggacgaa accatataaa atcattacaa
    56881 cgaaacacca gacgaaaatc ggactaattg catttactgc tccatttccg cttacttata
    56941 atccgaatgg ctgggacata cggaatcctt atgatatttt gccagaatta gtcgaagatt
    57001 tacgttcaca agtggatgtt ttagttttga tgagtcatct cgggattcag gatgatttcc
    57061 aaattgcaaa agaaatacca gcaattgatg ttattttagg atcgcacacc catcatttat
    57121 tcagagaagg tcacgtaatc aatgatgtcc aaatagcagc cgctggaaag tacgggcagt
    57181 atgtcggtga agtccattta accatcgatg aagagaaaaa aatccttaag cattcagcca
    57241 aagccatccc taccgaaaca atgacagctt ttacagaaga tgaacaagaa ataaatagtt
    57301 atctagaacg tgggcatgaa ttattaaaag aaaaagaagt agcagatttg ccattttctc
    57361 tgagtctaga tatcttcaat gagcattcgt ttatccaagt agcctttgag gcggtgaaag
    57421 aacgaggaaa gacggaagca gcaatattaa acagtggtct gtttttaact gaaattccaa
    57481 aagggcgggt caatcaggat cagcttcaca ctgcgttgcc gcatccgatg cacttgttaa
    57541 atgtcacatt gggtggaaat gatctgatcc gcttagtttt agaaatagaa aaaaatcgta
    57601 actttttacg taactatcct atgaagggaa tgggatttcg cggtaagatt tttgggcaga
    57661 tggtctataa tggaatcaca tatgattcta ttaaccatca agtcttttgg aagaacaagc
    57721 caatcgatct gaataaaaat tatactttta ctaccgtaga tcatttgatg tttgttccat
    57781 tttttccaac gattgagatt gctggaaaaa atgaattttt gttccctgaa tttatccgaa
    57841 gtgtggtagg agattacttg aaagctcact acccaatcaa ataaaaacaa ggtataataa
    57901 aaaagatagg tggtgaacgc atgtctgcaa aacaaaatga acagcagaac gttgagcata
    57961 aaacagaaat tgctaagaaa aaatcatcag ctgaacagga aacaacagtc accgaagaac
    58021 tgacagctgc tttggaagaa atcgttgagg agcctaaagc agaaaaagtc aaaggcgagc
    58081 tagtgacact gttagatgac agtaattttt tgataggtga aagacattat cggcttgtgt
    58141 ccgattatcg tgaaggattt aatgcagaaa agcttggaga acgttacagt gatgtactag
    58201 cccgatatga ttatatcgta ggagattggg gttatgaaca actacgccta aaaggatttt
    58261 tccaagcgga taatcgacgg gcgcatccag atcagcgaat cgattccttg gaagactatc
    58321 tttacgaata ttgcaatttt ggttgtgcgt attttgtcat cgagagaatc ggcggtaaaa
    58381 aagaaaagac aactcagcgc cgaaaaaaga aaaaaaacca taatcgtgcg tatatcgatg
    58441 agaaaaaagg accagtcgca tctaatcgca aaaaaccggt tattaaaaat cgaaaaacag
    58501 agtcagcaaa aacctctgaa aaagtaaaaa aacaagaaca acctaaacaa gtagaaaaaa
    58561 caaaaacagc atttacgatc agaaaaaggg aagagtagtg gatgaatgga ctataaagga
    58621 tatttaattg atttagacgg aacgatttat cgtgggacac aacccatacc ggccggtaaa
    58681 cgatttgtgg aagaattgca aaaaagaaaa ttacctttct tgtttgttac caataacacg
    58741 actaagtcac cagaaacagt agctaatcgt ctagcagatg agtttgacat tcatgtagct
    58801 ccagagacag tctacacagc tactttggca acaatcgatt ttatgaaagc agatggaaaa
    58861 gggaaaaaag tctatgttat cggagaagca ggattgattg acttgatttt agctgccgga
    58921 tttacatggg aagaagaggc tcctgactat gtagtggtag gcttagataa ttatctgacc
    58981 tatgaaaaag tagttaaagc aacacttgct attcaaaaag gtgctacttt cattggaacg
    59041 aacccagata agaatatacc tactgaacgc ggccttcttc ctggagcagg gtcagtcatc
    59101 tcttttgtag agacagcaac ccaaacgccg ccgatctaca tcggaaaacc agaagccatc
    59161 attatggata aagccgttga agtattaggg ctgcaaaaag aagaagtaat catggttgga
    59221 gataactatg aaacagatat ccaagctggg atacgcaata atatagatac tttgctcgtt
    59281 ttatcaggtt ttacaaaaga agaagatgta cctgggttgc ctatcccagc tacctatgtg
    59341 aaacaatcac tggatgaatg gagcttctaa tatgaaaaat tcgagatggc agtggatgga
    59401 atatgctgga ttgttcagcc tatttctgac actgatcagt ctggcagttg gtgtgacgat
    59461 caactttcga ccactatacg tatttgatat cggacatttg caaattttgg attatacttc
    59521 tttagatcaa gagacgttac tgaaaaattt tgatcaccta atgaattatc tcaataatcc
    59581 cttcaaaacc atcctcagct tgccagattt cccagtttct gctagcggag cccatcactt
    59641 ttatgaagtg aaaattttgt ttttagtgga ttatgctgtg tttttcatca ctttgatccc
    59701 gagtattttg tttatcaaat atttgcaaaa gaatgatcgg ctttggcggt tgattcgtcc
    59761 gtttcaaatc ggcatgttac tgccagttgt tttcggtttt ttcatgatga taggatttga
    59821 tcgctttttt atcctttttc atgaaacgtt tttcaacaac gatgactggt tattcgatcc
    59881 cgttactgat ccaatcatca atgtacttcc agaagaattt ttcatgcata gctttatctt
    59941 attttttgta ttattagaat tattttttgc tgtttttctt ttcttaggaa aaaatagctt
    60001 gaaacaaaca aaaaagaaag aactcgtcta aatagacgtt ttctttcttt ttttgttgaa
    60061 ttattgagta aggcgacttc ttttttctgg ttctttgtgt gctaatcgac tggcagcagc
    60121 agcggcgatc gctccgacga tatcatctaa gaaggtatgg acatgaacgc catcgtggct
    60181 gtttagttct tttaaaatac caggtttcac cttgtcgata taaccataat tcgtaaagcc
    60241 gatcgtaccg taaacattca cgattgacaa ggctaggatc tcatcgatac cgtaaaggcc
    60301 ttcgtcatct tcgataatat gttgcaacgg actcatcaat tttttctttt ctgctaatat
    60361 atctaattgg attccagtga taatggcgtt atgtacttca cgctttgtta gcacgctgtt
    60421 gacactttca gtacaatccg ccaaagtcaa atcagaaata tagtcttttt gtaaaaacat
    60481 gacaagttgt gcgatatctt ctacagttac gccacgttcc ttcaatagtt ctcttgcctt
    60541 ttcttctaaa gtctcagttt tgacgaccat attattgctc cccctttatt ttttctttct
    60601 ctatttcatg ttaacggaac atgaaaaaaa tgcaagaaac tgctttcggg tttaacttga
    60661 aatgcaaaga gatataaaaa atcaactagg acgaagattt ttatcgtata agcttgtgct
    60721 gtgaccaggc tgagttcgtt cctttggatt aatatagtgt aaagcgttgt tcactgcagt
    60781 aggtgcttca ccgagcccag tagcaatcag tttcactttt ccttcatagg tacagatgtc
    60841 accagctgca taaacgcctg gaatagaagt agacatatct gattttacag taatggcatt
    60901 tctggtacta tcaagtcccc aagaagaaag atgttctaaa ttagaagaga atccataatt
    60961 gacgatcaga gaatcaatca tcaaatcaat cgtctcgtca cttttcactt tttttaaccg
    61021 gatatctgtc agttccccat tattgcctga taatccatcg atgagatagg gagtaagcaa
    61081 gttgacagat gaagatttca agcgtgatac actatgttcg tgcgcacgaa attccgggcg
    61141 acgatgaatc aagtaaactt cgcttgcaat aggctctaac atcaacgtcc aatcgatagc
    61201 agaatcacca cctccagcaa tagcgacttt ttttccagca tacttcatca aatcattcac
    61261 gaaataatcc aatccgtggt tttcaaatga ttcagcatta tctaaattca attttcttgg
    61321 ctggaatgaa ccatttccta aagctaaaat gactgctttg gaataatgga tgcctttatt
    61381 tgtagtaatc tcgataactt cgtcttcacg agtaagcgag agaacttctt cttttaaatg
    61441 gaatgtatgg ttaaaagtag ttaattgttt ttctaattga tcgattaatt cacttgcttt
    61501 tattgctgga tacccaggaa tatcgtaaat atatttttcg ggatacaaag ttgctaattg
    61561 accaccaagt tgaggtagac tgtcaatgat ttttgtttta gcttggcgca ttcctgcgta
    61621 aaaagcggca aacatcccta caggacctgc accgatgatt gtaatgtcat agatttccat
    61681 aaaaattcct cccttttcac actatagcag aatttaattg agaatgcttc tcaaaaatgt
    61741 ttgaattcga aatcgaaacg gtaagttata gaatgtatct agtgatgaaa aataaaagaa
    61801 aacaaatcta gtatttttac gagaggttag gtattatgtt attattatta tgaagtatgc
    61861 aaatattagg aggaaacaat atggcattcc cacaactaga tttaaaaagt gaaaaaggac
    61921 caaaagctgt tatcaaaaca aaccgtggtg acattactgt tcaattattc cctgaactag
    61981 caccgaagac ggttcaaaat ttcattgaac tttccaaaaa aggttattat gatggcgtga
    62041 tttttcaccg cgttattcct gatttcatga tccaaggcgg cgatccaact ggaacaggaa
    62101 tgggtggcga aagtatttat ggcgaaagtt ttgaagatga attcagtcgt gaactgttca
    62161 accttcgcgg agctttatct atggcaaatt caggtccaaa cacaaacgga agccaattct
    62221 ttattgtaaa taacacaaat gtgccagcta atatgcttgg acaactagaa ggtgctggat
    62281 tcccatcaga aatcattgaa gcgtataaag gtggcggaac tccttggcta gatttccgac
    62341 atacagtctt tggacatgta ctagaaggga tggacacagt agatgaaatc gcaaatgtac
    62401 aacgtggtcc acaagaccga cctgtacatg atgtagtgat tgaaacaatt gaaatttcag
    62461 aatagtttgg tatggtacgg agtaattatg aaaaaggacg aaacctagta aggtctcgtc
    62521 cttttatttt gaataaatga tttaatcgat tgtctctaaa gcatacttga ttcgattaag
    62581 tccttccagt aaagtttctt ctggacaagc aatattcaag cgcatatgcc catgaccgga
    62641 aggaccaaac gtgatgccag gatttaatac gacttttcct ttccgaacaa gagtgttctc
    62701 caattcttgg tcgtctgaaa tataggcggt aaaatctaac cacattaagt aagttccttc
    62761 agggttcatt attctcactt tcggaagata ctgttcaaaa aactgattaa ctgtttgaac
    62821 atttttttct agataaggaa tcagttggct taaccattct ccacccgtct cataagccgc
    62881 ttgggttcca atcagtccaa aagtgttgat ttcgtgctgt tggttcatca ctaaatgttt
    62941 ttcaaaatca gctttcatct ctggattttt gataaatacc attgaatttt tgattgcagc
    63001 tagattgaaa gtcttggttg cggaagtaaa agcaactagt agatcatcta aattaggatc
    63061 aatcgttagc atggaagtaa atgtatgatc gaataaagtt aaatcttgat ggatctcgtc
    63121 actaaacaga atcacttgat gttttaaaca caattcgctt aactgtttca attcttcttt
    63181 gctccaaaca cgccctccag gattgtgagg attgcataaa atcattgctt ttacattgtt
    63241 ttcttcgatt tttttctcca tgtcagacaa gtccatgatg aaatgattat ttttttctag
    63301 caaagagctt cgaacgatgt gacgttgatt tgtttctata atagaggaaa atggaggata
    63361 aactgggtca tgaatcaaaa tagaatcgcc tggttttgta aaagtttgta ctgctgcagc
    63421 taaactggtt aacacaccac ttgtaaaaac gatattttct ttcgtcaact ccacagcatg
    63481 gtgttggcgt tcccaatgaa tgatagcctg atatagttta tcagacagag tggcataacc
    63541 aaagattcca tgtttgatgt attcactaaa cgctttagct accccaccgg gagacaagaa
    63601 gtccatatct gctacccaca aaggaagtaa gtcatccatt tgatatgtct cagcaattgc
    63661 gtcccatttt acactttctg tatcttttct tgaaatctgt ttatcaaatt cgatcatctt
    63721 aagacctcca gtcattttcg tcgcttcttt ttttgagcta tttacagtat aatgataaat
    63781 gcgaagagag ggaagtagat gacttataaa attggacaaa taattgaagg aaaagtgaca
    63841 gggatacagc cttatggcgc attcgtttct cttgataacg agacgcaagg acttattcat
    63901 gtttcagaag tacaatccgg ttatacgaaa aatatccatt cattgttaaa agtcggacag
    63961 ccagtaacag ttcagatcat cgacattgat gaatattcca aaaaaatcag tttatcttta
    64021 agaacgttag aaaaaacaaa tccagctgtt ccatatcgca gaaaaaggta tttcacgaat
    64081 aaaaatcgta agatcggatt taccactatt gcggagcagt tacctatttg gatagaagaa
    64141 gcattatctg aactagaacg gaaagaaaat aagaaataaa aagtgtattt tttctgaaaa
    64201 tgtgctagaa tttttatgta agccatagaa caagttgatc gaggtggaaa ttttggcaag
    64261 caaaagagtt gctggtacaa ttatgttgca tttggaggat ggtactaaaa aatttttggt
    64321 ccattcaatt gatgacaagt tggaatttgc gttagcggaa ctttctgaag gaaagactgg
    64381 tttagcgaat atacttaatt tcttaaaaga aatagttcat atcgatgtta gcaagattag
    64441 tttgatggaa ttgacaaata cacatattaa agaggaaaat gttccgttgt ttgtctttga
    64501 aactgaacaa aagaatcaaa gagaagaatt aggcgaagga tatatttggg aagaaccagg
    64561 tcaaatgcgc tctgttcttg gaacttacga agtagaggga gtgcctttct tctaaaatac
    64621 agattaagcg aacaaagaag tttgaatttt tttcaaactt ctttttttat tgtttggtcc
    64681 aaaaaataca atgaaatgtt ctgctaaaga tagtttttta gtgcatttta ttttcaaaat
    64741 atgttgaaaa cggttgtata acgaataata ataattgatc gaaaaatcat ttcacaaagt
    64801 aatgcttaat ttatagttga ccaaggaaga aatacatgat aaatttaaca gcgttgattc
    64861 tgaagtggat gttatatcac atttctaaaa aatttaaaaa a
//
LOCUS       DACEOM010000006        61939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEOM010000006 DACEOM010000000
VERSION     DACEOM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.006"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW781_000347"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW781_000347"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1181367.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW781_000348"
     CDS             complement(937..1518)
                     /locus_tag="IW781_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1181368.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW781_000349"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW781_000349"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1181369.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW781_000350"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW781_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1181370.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW781_000351"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW781_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1181371.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW781_000352"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW781_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1181372.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW781_000353"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW781_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1181373.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW781_000354"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW781_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1181374.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW781_000355"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW781_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1181375.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW781_000356"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW781_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1181376.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW781_000357"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW781_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1181377.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW781_000358"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW781_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1181378.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW781_000359"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW781_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1181379.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW781_000360"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW781_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1181380.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW781_000361"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW781_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1181381.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW781_000362"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW781_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1181382.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW781_000363"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW781_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1181383.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW781_000364"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW781_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1181384.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW781_000365"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW781_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1181385.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW781_000366"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW781_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1181386.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW781_000367"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW781_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1181387.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW781_000368"
     CDS             13972..14157
                     /locus_tag="IW781_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1181388.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW781_000369"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW781_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1181389.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW781_000370"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW781_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1181390.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW781_000371"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW781_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1181391.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW781_000372"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW781_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1181392.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW781_000373"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW781_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1181393.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW781_000374"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW781_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1181394.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW781_000375"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW781_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1181395.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW781_000376"
     CDS             18232..18879
                     /locus_tag="IW781_000376"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1181396.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW781_000377"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW781_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1181397.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW781_000378"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW781_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1181398.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW781_000379"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW781_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1181399.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW781_000380"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW781_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1181400.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW781_000381"
     CDS             20317..21255
                     /locus_tag="IW781_000381"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1181401.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW781_000382"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW781_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1181402.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW781_000383"
     CDS             21935..22774
                     /locus_tag="IW781_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1181403.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW781_000384"
     CDS             22750..23619
                     /locus_tag="IW781_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1181404.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW781_000385"
     CDS             23609..24406
                     /locus_tag="IW781_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1181405.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW781_000386"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW781_000386"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1181406.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25810..28479)
                     /locus_tag="IW781_000387"
     CDS             complement(25810..28479)
                     /locus_tag="IW781_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1181407.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28717..28905)
                     /locus_tag="IW781_000388"
     CDS             complement(28717..28905)
                     /locus_tag="IW781_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1181408.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29175..29537
                     /locus_tag="IW781_000389"
     CDS             29175..29537
                     /locus_tag="IW781_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1181409.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29589..31271
                     /locus_tag="IW781_000390"
     CDS             29589..31271
                     /locus_tag="IW781_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1181410.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31388..33424
                     /gene="recG"
                     /locus_tag="IW781_000391"
     CDS             31388..33424
                     /gene="recG"
                     /locus_tag="IW781_000391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1181411.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33473..34474
                     /gene="plsX"
                     /locus_tag="IW781_000392"
     CDS             33473..34474
                     /gene="plsX"
                     /locus_tag="IW781_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1181412.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34596..34838
                     /gene="acpP"
                     /locus_tag="IW781_000393"
     CDS             34596..34838
                     /gene="acpP"
                     /locus_tag="IW781_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1181413.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            35106..36110
                     /locus_tag="IW781_000394"
     CDS             35106..36110
                     /locus_tag="IW781_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181414.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            36111..37055
                     /locus_tag="IW781_000395"
     CDS             36111..37055
                     /locus_tag="IW781_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181415.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            37055..38017
                     /locus_tag="IW781_000396"
     CDS             37055..38017
                     /locus_tag="IW781_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181416.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            38044..38958
                     /locus_tag="IW781_000397"
     CDS             38044..38958
                     /locus_tag="IW781_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181417.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38984..40765
                     /locus_tag="IW781_000398"
     CDS             38984..40765
                     /locus_tag="IW781_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1181418.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            41113..41799
                     /locus_tag="IW781_000399"
     CDS             41113..41799
                     /locus_tag="IW781_000399"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1181419.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41819..45400
                     /gene="smc"
                     /locus_tag="IW781_000400"
     CDS             41819..45400
                     /gene="smc"
                     /locus_tag="IW781_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1181420.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45409..46218
                     /locus_tag="IW781_000401"
     CDS             45409..46218
                     /locus_tag="IW781_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1181421.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46229..47227
                     /gene="ftsY"
                     /locus_tag="IW781_000402"
     CDS             46229..47227
                     /gene="ftsY"
                     /locus_tag="IW781_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1181422.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47414..49681
                     /gene="gshAB"
                     /locus_tag="IW781_000403"
     CDS             47414..49681
                     /gene="gshAB"
                     /locus_tag="IW781_000403"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1181423.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49770..50222)
                     /locus_tag="IW781_000404"
     CDS             complement(49770..50222)
                     /locus_tag="IW781_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1181424.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50372..51322
                     /locus_tag="IW781_000405"
     CDS             50372..51322
                     /locus_tag="IW781_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1181425.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51315..52274
                     /locus_tag="IW781_000406"
     CDS             51315..52274
                     /locus_tag="IW781_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1181426.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52271..53026
                     /locus_tag="IW781_000407"
     CDS             52271..53026
                     /locus_tag="IW781_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1181427.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            53049..54002
                     /locus_tag="IW781_000408"
     CDS             53049..54002
                     /locus_tag="IW781_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1181428.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54646..54942)
                     /locus_tag="IW781_000409"
     CDS             complement(54646..54942)
                     /locus_tag="IW781_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1181429.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            55297..55653
                     /locus_tag="IW781_000410"
     CDS             55297..55653
                     /locus_tag="IW781_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1181430.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55663..56562
                     /locus_tag="IW781_000411"
     CDS             55663..56562
                     /locus_tag="IW781_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ1181431.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            57079..57810
                     /locus_tag="IW781_000412"
     CDS             57079..57810
                     /locus_tag="IW781_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1181432.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            57955..59982
                     /locus_tag="IW781_000413"
     CDS             57955..59982
                     /locus_tag="IW781_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ1181433.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            60090..61187
                     /locus_tag="IW781_000414"
     CDS             60090..61187
                     /locus_tag="IW781_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1181434.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            61494..>61939
                     /locus_tag="IW781_000415"
     CDS             61494..>61939
                     /locus_tag="IW781_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1181435.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        20459 a        11084 c        13212 g        17184 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaaggaag aggtcgtgaa atcagcgttt
    25501 tgacctgagt attagagaaa caaacgggaa aatagctcct catattttgc cgtttgttga
    25561 gctaataccg aaggtcagct gattgaacac cgtttaagaa agaggtcgtg agcctgtcat
    25621 ttaactctga gtcacaaaga gcaatttcaa aaaacagctt ctcatctttg atgaatatcg
    25681 tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt ttttctgtaa
    25741 ccaaaaaacg atggaacgtt gttttgattg cccttggtgc ggacagtctg aacaggttcc
    25801 atcgtttctt tattttctac gtcttcttct gtgtgtgttt cgtggttgtt cttctattgc
    25861 tggatcaatc gtgccataaa aggggcgatt gcggtcaagg taccagacat aaagaataaa
    25921 caatgcaata cataggacaa aaaggattgc acctatcgta aatccttcag gcaagtaact
    25981 gaaagtgaca gtatgtttac ctgctggaac ttttatcatc aagaatgcgt ctttgaaatt
    26041 gctgaccttg actggtttcc catcaatttt agctttccat cctttgtcat aaggaatagt
    26101 tgtcaccaac atggaatctt cagcggtatt tacagtccca gaagcttttc tgccagtggc
    26161 ttccatttcg acaccttttt ctttgatctg attcattgcg gcttgatagg ctacaacatc
    26221 tagaccaacg actttaggct ccatgaaact tatttctttc gtcccataaa aacttgcagt
    26281 aaatgtcact gttgtctctt gaggataata gccaagatca tagtattgac cagaaatgtt
    26341 gatttgcgtt tttcgcgatg taccattgac agtgatcgtc gcactagaac tttctaattg
    26401 cgcaaaattg gttgggaaca aacttaagta agcttgtgta tttgcaggca catggacttc
    26461 ccaagtgagc tcttttggct ggttatctgc tgtttctgag taagtgacgc cagtcgttgt
    26521 ctttgtgatc gtcgtattca actgattctt caaagtaggt gtatagaatg taaaatactg
    26581 ttcatttaga ccagacaatg tattgatcaa gtttgtctga ctggtcaaat tatctgtcac
    26641 tggctgctca acatcattaa cggacaacgg tgcagtaaat gctagtggca atgcattgac
    26701 attctgatac agtgtatagt cgccgctttg accagcttgg cggaagccat atttactgaa
    26761 cgtgctttcg tttttagaaa tgttatattt gattcctgta aacccatcca tcagcaaagt
    26821 attattgtta taacggatat tcaaatttgt ccctcttgaa cggaaaccta atttatccag
    26881 gtaagaggaa gaattacgat ttcttataga agaaaacaaa ctgataccac tgtaaccata
    26941 gttgatcgaa tcgtttgcag atactggatc cagattttcc aagcggtaaa acgagtcgga
    27001 atcttttttt gtttcattta caagagcttt caagtcagga taaggatcag tatataaact
    27061 gcgggaagca taattccaat catcaagaat gccatggatc atactgttcg tattgaccag
    27121 tgcttcagca gacatgatca ataataacaa tatactcaga tgattgaatg aaagtttttt
    27181 atgctgataa aatcccacag caaaagcata aatccctaag aaaaccactg tcaatacaaa
    27241 ggaagtcatc gtcacatagg tgtagcttcc ttcaggtttg atgccccatg ccaccgcaaa
    27301 gagagcagct acgaccaaaa tgattcctaa caacagccca cgatcgtctt gttctatctt
    27361 ttcccatcct tttcctgcaa gcaggagcac taagaaagag aacagatagg cataacggaa
    27421 taaaaacata ttcggtgcat gcataccatg ccagaacaaa ttcaatggaa caagataaaa
    27481 acttgcgata agtatggcga aaagactgcc gtacaaaaat ttatttttca acggtacttt
    27541 ccgactggca aaataaaaca gacagaggac taatggtagc agacctgcgt aaacgaacgg
    27601 aatcgatccg tattttgtcg tgtcataaac accaatcata tttttcatga ttagatccag
    27661 ataagaagtc gcttctgttt tcaacgtagt gatctgactc agtgcctcgc catttgaccg
    27721 aagatccagc actgctggca atacaatgat cattgaagcg ccgcctgcca gtaaagaagt
    27781 gataccataa ggaacaatcg tttttttata ttctgaccag tttgtcaata gacgggcgat
    27841 aaaatataag aaagaaaata cgccaatcat aaaacccata taaaaactgg ataaaaacaa
    27901 gagtaaataa ctgataaata aaagtttcgg ccgtttaaga tccataacac gatgaatccc
    27961 caaaatcact aatggaagat aaaaaaatgc atccagccac atgatgatct ccgaatgtgc
    28021 agtgataaat gagatcaatg cgtaactgac gctaagtgca acatgccccc agcgaggaat
    28081 cttgaacgta cgtgcaagaa accaaaaagc caatccggca cagccgacct taagcaaagt
    28141 cagaacatac agtgcatctg gcatcatctg gtttggaaaa aagaacacca gtggcgtgaa
    28201 gatcccgcct agatagtaag aaattaaaga taaataattc aatcctagag aagcattcca
    28261 tgtataaaaa atactttgct ttccaagaag aacatttcgg aagctggcat gaaaattaga
    28321 aaactgtgaa aaggcatcac ttgccaaaat acttctgctg cttccaggat agattccgat
    28381 acttagataa acaagtgcta aaatcaaaaa cggaatcagg aaactagctg ctgcgtagcg
    28441 tccgttttct ctaaagaatg actttaaatt ttttttcata tttattttcc accaaatttc
    28501 tatgaagttg tattcagttt atcatatcct tgtttttctc tgcttaaaaa gagtctagtt
    28561 ttcacaaaaa aataaaattt agaataaata acttcataat ttgttcgtat aacaaaaata
    28621 aacgcaaaaa aagggtagga atcaaaagtc ccacccttaa aaatttttat taaataaaaa
    28681 accgttcact atctgaacgg tccaagtatc attcaattaa acgcgttcaa cttttcctga
    28741 tttcaaagca cgagttgaca cccaaacttt tttaggtttt ccgtcgacca taacgcgaac
    28801 tttttgtaag ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg aacggttgtt
    28861 tccacttttt gtcttgcgac ctgtaaagta acaaactttt gccatggtaa tttcctcctt
    28921 tgccttgatt tggagcaata gaataattca gctcatacca gactaacata tcatatagaa
    28981 tgggaatttg caagtacatt ttcatagata caattgacaa ttgagaagaa taattgtaaa
    29041 ttaaacaagg ttatacctga aattttgcaa ggtttttgag tactttgggt aactgccttt
    29101 tatttcaagt tgcgctatga taaaatatga tagaagaaaa atgggagttt tttacccgtt
    29161 aaggaggctc attcatggct gtaaaaattc aaacgtcagc tggaaccatt gaaattacca
    29221 atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt gttgtaggaa
    29281 tggccagcaa aaatcaaatt aaagataata ttactgagat tttgcgtaaa gaaaattact
    29341 caagaggagt agttgtccgc caagaagata atggtattgc tgtagatgtt tatacgatta
    29401 tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa gtgaaataca
    29461 atctagaggc attattaggc gtaactgcca attcagttaa tgtctttgta caaggtgttc
    29521 gtgtactgcc agactaggca ggagactggc tggtaactca gcagtcgatc tgaaggagga
    29581 ttatctaggt gagtgtaaca gaaataagtg caagccagtt ccaggaaatg gtccaggcag
    29641 gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc attaaatgta ttccctgtgc
    29701 cagatggcga tacgggaaca aatatgaatc tatctatgac tagtggagct aaggcagtag
    29761 tagattcaac ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa ggattgctga
    29821 tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga ttttctaagc
    29881 aaattccaga tgttgtctca ttaaatgctt ctgatcttgc tgcagccttc actcatggag
    29941 ttgaaactgc gtataaagca gtaatgaaac cagtagaagg aacgattttg acagtagcta
    30001 gagaagcagc gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc gttgaagtaa
    30061 tgacagctgt tgtaaaagga gcaaagcgtg ctttagctaa aactccagac ttgcttccag
    30121 tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg actgctgttt gtttatgagg
    30181 gctttttaag tgcattgaat ggagaattcc aagcggatga aacatatgaa ccttctccag
    30241 cagaaatgga tgatatggtc aatgcagaac atcatcgcag tgtccaaggt caaatggcga
    30301 cagaagatat ccattttggt tactgtacag aaatcatggt aaaaatcgga gaaggaccta
    30361 cagtcgatag tacattcgat tacgaagaat tccgtaacta tttagatggt ataggagatt
    30421 ctttacttgt ggtcaatgat gacgagatca tcaaagtcca cgtgcataca gaaaacccag
    30481 gagaagtctt gaattatgga caaaaattcg gttcattagt caaaatcaaa gtcgacaaca
    30541 tgcgcttgca gcatgagacg attttggaac acgatgaaga agtgaacgag tttgcgaaaa
    30601 aagaaatacc acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa ggcgtccaag
    30661 agctgtttga aagtttaggc gctaattatg ttatcagtgg tggacaaaca atgaatccaa
    30721 gtacggaaga cattgttaaa gcgattcaag aagtaaacgc agataaagtg attgtcttgc
    30781 caaacaataa gaacatcttc atggcagctg atcaagcagc agaagtagca gatatcccag
    30841 tagcagttgt tccttcaaga acgatttccc aaggattgac agctatgctt tcattcaatg
    30901 atcaagcttc tttagaagaa aacaaagaaa cgatgacaga aatgttgtct agcgttgtga
    30961 gcggtcaagt cactcacgcg atcagagata cctcaattga cggtgtagag atcaaagaag
    31021 gagacttttt gggcatgatc gatggaaaaa tcctgatctc taacccagat atcacagcaa
    31081 cagcgttaag tacactagaa caaatgatca atgaggacac agagatcgta acgatcttgg
    31141 caggtgatga aggaacagtt gaacaagcaa atgaatttgc tgagaaacta acagaaaaac
    31201 atcctgattt ggaaatcgag atccaccaag gagatcagcc tgtctatcct tatctatttt
    31261 ctgctgaata atttctgtgt cagaatatct ttctaacaca gtattcattc ataaaaggcc
    31321 ttcggttagt gatcaggtat gatcgctgac caagtccttt ttttgttaaa ggagggagga
    31381 aaaatttatg cgttctttag atgatccaat cactatgctg gcaggagtcg gagaaaaaag
    31441 agcagctagt cttgccagct tgggcatcca aacgattgaa gacttactga cttattatcc
    31501 ttttcgctac gaagatattc aggaaagaaa tctaaacgag atccaggatc aagaaaaggt
    31561 tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt ataaaaaaag
    31621 ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt tttttaatca
    31681 accttatcta aaagaaaaag tcattttatc ggaggaaatc gccgtatacg gtaaatggga
    31741 tgcaaagcgt aaagccttga acgggatgaa gattttaggt tctcaaacaa tggatgactt
    31801 ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg acattagttg atctaatcaa
    31861 aaaagggttt caagaatacg gcacattgat tgaagaaaat ctgccaaaca accttgtgga
    31921 gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt atgcattttc caaaagacca
    31981 cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa gagttctttt tatttcaaat
    32041 gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg agatccttta
    32101 tgatgtccaa aaattgaaac aatttactca aaagctacct tttgaactga caaatgcaca
    32161 aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata tgcagcgact
    32221 tttgcaggga gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt tatacgctac
    32281 cgttacagca ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg cacaacaaca
    32341 tatggaaagc ttgcaacagc tgtttgatcc caatgaagta accatcgcat tgattacggg
    32401 gtcaacgaaa gcaaaagaac gacgtgagct ccttgaaaaa ctagagcaag gggaaattaa
    32461 cgtcgtgatc ggcacacatg cattgatcca agatggagtc gaatttcaga atttaggtct
    32521 agttattacg gatgagcagc accgttttgg cgtgaaccag cggaaagtac ttcgtgaaaa
    32581 aggctggcga ccagatgtct tatttatgac agcgactccg attcctagaa cattagctat
    32641 tacagcatat ggtgagatgg atgtatctat cattgatgaa cttccagctg gacgtatccc
    32701 gatcgaaaca cgctgggtaa gaacacctca attggacagt gtactggatt ggacgaaaaa
    32761 agaactggca agaggacatc aaatgtatgt catctgtccg ctgattgaag aatcagaagc
    32821 attagatgta aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat ttgcaccaaa
    32881 atatgaagtt ggcttgttac atggtaaaat gaagaatcag gaaaaagaag taatcatgga
    32941 agccttcaaa gacaaccaga tgcagatatt agtttcgact actgtaattg aagtaggggt
    33001 aaacgtgccg aacgcgaccg tcatgctgat catggatgcg gatcgatttg gattggcaca
    33061 gctgcaccaa cttcgtggac gtgttggacg cggaagtgat gcttcctact gtatcttagt
    33121 tgctaatcct aaaaatgaac taggtgtgga acggatgaaa atcatgacag agaccaccaa
    33181 tggttttgta ctaagcgaaa aagatctgga attacgtggt cctggtgaag tatttggctt
    33241 ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg gtgacggatg gaaatgtttt
    33301 ggaggtagcc agagaagagg ctgcagccct ttggcaaata aaggattggc agatgcttcc
    33361 tgagtatcag cctttagcgc aaagcctcca aatagaaaat caggataatc gttatttcga
    33421 ttagcagact gtgctatact ttatactgat gacggaaaaa ggagtggaaa caatgaaaat
    33481 tgccgtagat gcaatgggcg gagatcacgc acctcaagca attgtagaag gcgtggcttt
    33541 agcgcagcaa gattttccag aagtagaatt cttactttac ggaaaagaag cagaaatcaa
    33601 gaaatactta acaaatgaaa aaaacatcac gattatccat acagacgaaa aaatcaatag
    33661 tgatgatgag ccagtaaaag caattcgacg aaaaaagact gcatcaatgg tccttgctgc
    33721 acaagcagtg aaaaacggtg aagcagatgc tgtattttca gcagggaata caggcgcgct
    33781 actagcagca gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc ctggcttgat
    33841 gtctaccttg ccagtagtag ggaaagaagg tgccggtttc gatatgctgg atcttggcgc
    33901 taacgcagaa aacaaaccag aacatttgtt gcaatacggt attttaggtt ctttttatgc
    33961 acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg gaacagaagc
    34021 aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg aaacaagctt
    34081 gaattttatt ggaaacgtag aagctcgtga gctactcaat ggtgtggctg atgtagtggt
    34141 cacagatgga tttacaggca acgctgtttt gaaatctatt gaaggaacag caatgaatat
    34201 gatgaatcta ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa tgggcgctat
    34261 gcttttaaaa gacggcttac gttctttgaa agcagaaatg gattattcca aacatggcgg
    34321 tgccgttttg tttggcttga aagcaccagt cattaaaact catggagcaa caggccccga
    34381 tgctgttcgc tatacgatcc gccaaatcca cacaatgctt gagacagatg tagttggaca
    34441 attagtcgaa caatttgaaa aaagcgaaga atagtatttg gatatctgaa gcgatccgtt
    34501 ccattgaaaa aaaggtggac attcctgaaa aatgccagta aaattagcca tggtgtaggt
    34561 aattgacaag tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa gtatttaaca
    34621 aagtagcgaa aattatttca aatcattttg agatagatac cgataaagtg acggatgaat
    34681 tgaacatcaa agatgattta aaagctgatt caattagtat tatggaattc gttttagagc
    34741 ttgaagatga attcggtacg gaaatttcag atgaagacgc tgaacaaatt gaaacagtcg
    34801 gtggcgcagt agattatatc agcagccatc ttaaataatt tcaaataaaa gcaaagaagg
    34861 ataaaaagct gcatgcagct ttttatcctt ctttttttaa tctttttgac acgtaaaagt
    34921 catttttctg ataattctgg cgataaacag aaattatgtt gcaaagtaaa gcggttttct
    34981 atataattat taataattca ttacacaaag ataagaataa cctttgttat aaaacgattt
    35041 attctgtgta atgtcctttt ttattaagaa aaacttccat ttaggaagca gagaggggta
    35101 ttcgattgtc cgataaccaa ttattagaag ttcagaacct acatacagga ttccgactca
    35161 aagatgacta ttacgatgca gtagatgacg tttcatttac tttagacaaa aacgagattt
    35221 tagcaatcgt tggggaatct gggtgcggga aaagtacctt ggctactacg attatgggat
    35281 tacttgatcc taataacaca aaaattacag gtgagatttt atataacgat ctgaatttaa
    35341 tcgacctgaa cgaaacgctc tataataaaa tccgcggaaa tgatatcgga atgattttcc
    35401 aagatccgtt gtctgcatta aatcctctca tgagaatcga agaccagatc aaagaaggac
    35461 tttcctatca cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta gaattgctag
    35521 aacaagtagg gattcctaat cctgcgagag ttggacgcca atatccccat gaactatcag
    35581 gcggtatgag acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct ccaatcgtca
    35641 ttgcagatga accgacgaca gccctagatg taaccatcca agcacagatt cttgatttac
    35701 tgaaagacct tcaggaagaa acaaaaacag ggatcatctt gatcactcat gacttaggag
    35761 tcgtagcaga aatggctgat cgagtggcag tgatgtatgg cggacagttc gttgaagtag
    35821 caggtgtaaa agaactgttt gatcatccta agcatcctta tacacgctct ttactgaact
    35881 ctataccaca agaaggaaat catgatgcag aactccatgt gatcgaaggt gtcgttcctt
    35941 ctctgaaaaa catgcctcgt aaaggttgtc ggtttgcacc acgtattcca tggataccag
    36001 caagcgatca tgaagaagag cctatccttc atgaagtcgc accgaaccac ttggtacgtt
    36061 gctcctgcta taagcatttc cactttagag acgaaaaagg agaggtgtag atgacagaat
    36121 taatcacaat caaagacctg aaagttcatt accctattcg cagtggattc ttcaatcgag
    36181 taacggatca tgtatatgct gtagatggtg tggattttat tatcgaaaaa ggaaagacat
    36241 atggtttagt gggagagtct ggctctggaa aatctactac gggaaaagcc gttgtgggat
    36301 tagaaaaggt aacagccgga caaatcatgt atgaaggaaa agatgtaacg aagcgaagca
    36361 accgcaaaaa aatgggttat aacaaggatg tgcagatgat cttccaagat tcaatgtcca
    36421 gtttgaatcc taaaaagcga gtgttggata tcattgctga acccatccga aattttgaac
    36481 ggttaagcga tcaagaagaa aagaaaaagg taaaaggact gctagatatc gttgggatgc
    36541 cggaagatgc gctatataaa tatcctcatg aattttcagg cggacaacgt caacgtctag
    36601 gagtagcccg tgctgttgct accaatccaa aactgatcgt agccgatgaa cctgtatctg
    36661 ctctggatct atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc cagcaagaat
    36721 acggcctcag ctatttattc atttcacatg atttaggtgt agtaaaacac atgtgcgaca
    36781 atatcgcaat tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa gacatttata
    36841 atgacccgcg acatatctat acgaaacgtc tgctttcagc tattccgcaa atcgatgtag
    36901 aaaataggga aagccacaaa gaaaatcgac gaagagtaga acaagagtat atccaaaacc
    36961 aaaaagaata ctatgatgcg actggtcgag tctacgattt gcgtacgatc acaactacac
    37021 acaaagtggc gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc tgcgacgtgt
    37081 tttattgatg atcccgcaag ttattatttt aagtgttctg atcttcttat tagcccaagc
    37141 gatgccgggg gatccattta ccggtttgat caatccaaat caggatccgg cagtgatcga
    37201 acagatgcga caagcagctg gcttgaatga cccatggtat gtccagtact tccgttggat
    37261 cggaaatgct ttgcaaggcg attttggtca aagtttctta tacaaacgct ctgttgctga
    37321 cttgatcggc gaacggatca tcaacacttt atatctatcg atcttgacag ttgtccttac
    37381 ttatttgatt gctgtgcctc tggggatgct tgctggccgg tatcaaaatt caatcttgga
    37441 taagattgtc gtaatctaca acttctttag ttttgctgtt cctttattta tcttcggctt
    37501 gatcatgcta tttgtttttg gttatcgttt aggctggttc ccaacaagtg gatcacaaac
    37561 actaggtttg caaggtggat ttatcactca atggatagac cgtttgaaat atattctgtt
    37621 accatcaatt acccaagcat ttttggcgac tgccgtcaca atccaatatc tgcgaagtga
    37681 agtaatcgat tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag gggtaccgac
    37741 gaataaagta ttcagtcgac atattttccg aaatgcagcg ttgccgatgg cttctcaaat
    37801 ggggtatgaa atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga tttttggtta
    37861 tccgggagtc ggaaaactat ttattgattc catcggccaa cgggactata ccgtgatcac
    37921 atccttagta ttgattttag gaatcgcgac gctagtgggg acattacttt cagatatcat
    37981 catgagtatc gttgacccgc gtattcggat tcaataaaac caatcagaaa ggagaatgga
    38041 tcgatgagtg aaaaaaacaa tacagcagtt caagaaagta ttcctcctat ggggatacga
    38101 atgattgctc gagaatttaa aaaagataaa attgctattt tttcattagg tttgctagtg
    38161 gttttattat tagtgatctt tatcggtgcg atgctgaccg atcaagacaa agtgatgtat
    38221 gttagtatct tcgacaaata cgctgcacca ggaacagttt cgacacaagg acaaaagttc
    38281 attctgggag cagatgaagg cggacgtgat gtattaggac aattgatcat aggtgctaga
    38341 aactctgtta cgatcggatt tgcgatcacg atccttactt cgatcatcgg ggttggatta
    38401 gggatcatct ctggctacta cggagggatc attgacaaca tcttgatgcg gatcgttgat
    38461 tttatcatga ttctaccgat catgctgatc attatcgtct tcgtatcgat tatttccaga
    38521 tacaatgtct ggtcctttat attcatcatg agtgcatttt attgggtagc taaagcccga
    38581 ttattccgaa gcaagacatt atctgaagca agaagagatt atgtcagtgc ctcaaaaaca
    38641 atgggaacca atgattttaa aatcatgttc cgcgaaatca tgccgaatct aagttcatta
    38701 atcattacta atttgacgat gaactttgcg gcaaacatcg gtatcgagac aactttgact
    38761 ttcttaggtt tcggcttgcc ggcaaatgta cctagtttag gtacattgat cggttatgca
    38821 agtaacggag aagtcttagt gaatcggcaa tggatctggt tacccgcatc aatccttatt
    38881 ttagttttga tgttgagtat caattatgtt ggacaagcat tcaagcgttc tgcagatgcg
    38941 cgccaacggt taggctagta tagaagcgaa gggggaaata ggaatgaaga aaacgatttt
    39001 tggatttgca gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg gaggtacaga
    39061 tacctctgga aacaactctg gaggcaacgg caataacaaa gaggaattaa cttttccaat
    39121 tgcaacaaaa aataatgatg atgcaatcga agacggcgaa ttagaagctg cggtcgcaac
    39181 ggacagtcag tttaaaggat tgttccagtg ggagttttac caagattcat atgatgcagc
    39241 ttttatggct ccttctcatg aaccgctatt tgcaaacgat gacgatttca gaatcacaga
    39301 cgacggagct gcaacgcttg atctagatga agatacgaaa aaagcgacaa tcaaagtaaa
    39361 agacaacgtc aaatggtcag atggtgaaga tgtaacggcg gacgatgtga ttttccctta
    39421 cgaaatcatt gggaataaag attacactgg tatccgttat gacgacactt tccgtaacat
    39481 cgtaggtatg gaagaatatc atgatggaag tgccgacaca atctccggaa tcaaaaaaat
    39541 agacgacaaa acagttgaaa tcgaatataa agaagtaaac ccaagcatgc ttcaatcggg
    39601 tggtggtatc tggtcatatg cagcaccaaa acatacgtta gaaggtgtag caatcaaaga
    39661 tatggaatca agcgatcaag tacgtaaaaa tccagtgact tttggccctt attacatgag
    39721 taatatcgtt gcaggagaat cagtagaata cttgccaaac gagcactact ggaatggtac
    39781 gcctaaattg aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa ctgaagcatt
    39841 gaactctaaa ttatatgata tgattttcca aatgccaaca gatacttacg atacctataa
    39901 agaaattgct ggctatacaa acttaggccg tgaacaaact tcttatactt atctaggatt
    39961 caagttaggt aaatggaatg cagatacaaa gagtgtggaa tacaatccag attcaaaaat
    40021 ggctgataaa tcattacgtc aagcaatggg ctatgcatta gataataatg cagtaggtga
    40081 acgtttctat gcaggtttga gaagcaatgc gacttcattg attcctccag tattccagtc
    40141 ataccatgat gatgagatga aaggattcac acaagatcta gacaaagcga aaaaactatt
    40201 agatgatgct ggctacaaag atgtggatga cgatggttta agagaagaca aagatggaaa
    40261 taaactaaca atcaactttg cttcgatgtc cggtggggaa acagcgcaac cattggcaga
    40321 ttactatatc caacaatgga aagaaattgg tttggatgtc caatacacaa caggtcgtct
    40381 gatcgaattc aattctttct atgataaatt agaaaacgat gatcctgaaa ttgatgttta
    40441 ccaagctgct tggaatacag gaactgatcc aaatccaagt ggactatggg gaccaaatgc
    40501 tgcgttcaac tacactcgtt atgaatcaga agaaaataca aaattgatca acgatatcag
    40561 ttcaagcaaa gcatttgatg aagagtacca aaaagaagca tatcaaaaat ggcaacaata
    40621 tgcatttgaa gaagcatttg cgattccaac gttgttccgt aatgaagtat tgccagtcaa
    40681 cgaccgtgtc aaagactggg attggtctta tgatgtacca aacccatggg caaatgtttc
    40741 tgttacagca gattcacgtt cataatcact aaaaatgacc gaaaaagggc gatgtccttt
    40801 ttcggttttt tgatattttt ctgtttattt ctctaatatt caggtcaagg cgctgatttc
    40861 atgacttctc caataaacgg tgttcaaagt ccagtttctc ggtattaagt caaatttatg
    40921 caaaaatatg gaaagccatt ttcacaaaat tttttcttca tactcgaaac taaacgaatt
    40981 tttcacaact tctcgtcaat cactaggatt attaaagctt tacttacctt ttgaccgaac
    41041 catttcaaat ttgacaaaaa accagtataa taaggaagta tcgaaaaaat atccataaaa
    41101 ggaatgtgga aaatggacaa ccagctgaca aaagaactaa aagaaaacta taacatcgtt
    41161 tttcacgatc taaatttgct tgaacaagct tttacccatt catcctatgt gaatgagcat
    41221 cgcaatctgc aattatcgga taatgagcga cttgaatttt taggagatgc cgttttagaa
    41281 ctgatggttt ctcagtattt attccgttta tatcctcatt tgccagaagg aaaattgaca
    41341 aaaacacgag ctgcgatcgt tcgcgaagac agcttgtcga aatttgccaa agaatgtcac
    41401 tttgatcaat atgttttgtt gggcaaggga gaagagaatt caggaggacg tacgcgacca
    41461 gcattgcttt gcgacctatt cgaagctttt ttaggcgcgt tatatcttga ccagggattc
    41521 gacacgaccc atgcattcat cgaaaaagtg atttttccta aagtcaaagc aggtgctttt
    41581 tcacatgaga tggatcataa aacaaaacta caagaagtct tgcaaaaatc aggtgatgta
    41641 tcaatcgaat accgattgat caatgaagaa ggtccagctc atgaacgagt attttggatc
    41701 gaagtttatg tagatgatca attgattggc actggacaag ggaaatcgaa aaaattagca
    41761 gaacaagctg cggcggaaaa tgcattggca gcactgtaaa agtaagtgag ggaaagcttt
    41821 gtatttgaaa cgaattgaaa tagccggttt taaatcattt gcagacaaga cagtcattga
    41881 ttttgaaaat agcgtcacag cagtggttgg accaaatggc agcggaaaaa gcaatattac
    41941 agaggctatc cgctgggtat taggagaaca atctgctaaa agtctgcgag gcggaaaaat
    42001 gccggatatc atctttgccg gttccgatac ccgtaagcag ctgaatattg cagaagtcac
    42061 agtgatttta gacaatagtg atcattatct gccattggag tacaatgaga tcagtgtaac
    42121 ccgccgttat cgacggactg gggaaagcga gtttttcatc aataaacaaa gctgcagatt
    42181 aaaagatatc caagaattgt tcttagattc tggattagga aaagaatcgt tctcaatcat
    42241 ttctcaagga aaagtagaag cgattttcag tagtaaaccg gaagaccgtc gtgggatctt
    42301 tgaagaagca gctggtgtct tgaaatacaa gcagcggaaa aagaaagcag aacagaagct
    42361 ttttgaaaca gaagacaacc tcagccgcgt ccaagacatc atccacgaat tggaagaaca
    42421 gttaactcct ttggccgccc agagtgaggc agcaaaggag tttttacggc taaaagaaac
    42481 actgactcaa accgatgtct ctttgatggt tgctgaaatc aagacagcaa aaaaagattg
    42541 ggacaacaaa caagcacagc ttgcgaaatt caatctagaa ttaggcaaac tatctgagtc
    42601 gatccaagaa caagaatcca ttttggcgaa gcagcgtaaa gaaaatgcgc aagccgatcg
    42661 tctgatcgaa aaaaatcagc aagtattatt agatctttcg gaaaaactga agcagacaga
    42721 aggacaaaaa gatgttttgc aggagcggac aaagcataca caaaaaagct cgcaagaata
    42781 tcaaacttca ttagcagaag cacaaaaaaa ggttaaacat tttgaaaagc ttcaagaaag
    42841 cttgatgaaa gcagcagcag aaaaagaaac agagatccaa aaagctgaag caaatctcat
    42901 aaaaacacag caagaattag aaaaatacca aaaatcaacg aaagaattat tggcagaatt
    42961 aagagatcaa tatgttgatt tgatgcaaga acaagcagct gtaggaaacg aactgaagta
    43021 tctagaacgt caatatatac aagaaacagc aaaaagcaag cagacgttag ccaagcaatc
    43081 ggaagtagaa gcatcagtag accgattgat cttacaaaaa gaagaattga cacaaaagca
    43141 ggcgcagcta aaatcatctt taactgaaac aaaagagaag ttagaaatga tccaacaaaa
    43201 cgggaaaaag ttccaagaag cattagcaaa agaacagcct aaaatgtatc aattaatgaa
    43261 tcaagtccag cagctacgtg ctcgtaagaa aagccttcaa gaaatccaag aaaactattt
    43321 tggtttttac caaggggttc gattggtctt gcagcataaa cagcagcttt cgggtattgt
    43381 cggagcagtg gctgaactga tcgatgttcc tgcagatttc acgttagcta tcgaagcagc
    43441 tttaggtggg gcagcccagc atgtcatcgt tgaaaatgaa aaagatgctc ggcaagccat
    43501 cacgtatttg aaacagcaaa gaggaggaag agcaacattc cttccactga cgacaatcaa
    43561 accaagacaa ttaccggctc atatattgac acaagcagca gctgtagaag ggtttatagg
    43621 gattgccagt gaacaagtca gttatcccga tcaaatccaa actgtggttc aaaacctgct
    43681 aggaacgatc cttttagcca aagacttaac tagtgccaat gcaatcgcgc aaacgatccg
    43741 ctatcaatat cgagtggttt ccttagaagg tgatgtgatg aatgccggag gatccatgac
    43801 aggtggagcg aataaacgag gaaaccaagg cagtttgttt gtacaaaatc aagaattgaa
    43861 acagttgact agtgaatttg aagaagcaga taaacaactc caagcacaag aaaaaaaagt
    43921 tcaggagctg caacaagaaa cagctcgttt agccgaagaa caagaagttt tgcgtacacg
    43981 aggcgaacaa ttacgttttg aagaacaaga agcgacaaat cagctacaaa acatcataaa
    44041 tgaacttgaa cgttttgaaa aagaaaagca aatctcaact tttgagacac gtgaactgca
    44101 gcagtttatc gaagattacg aaaagcaaac gaacgaattg aaagacaaac aaacagatct
    44161 tgaatcgcaa cgacaaaaaa tcgacgaaga aatcaaatcg ttaagtcaag aaagcgacca
    44221 gatggaagcc agaagagcac aagtacaaag tcaaaaagca caagaacaag cagatcttgc
    44281 tgtcttgaaa gagcagttta atcatctgca gatccagctc cgcggtgccc gagtccaaaa
    44341 agcagaggca accgaacgtc aagaagcgat agaaaagcag ttggcgacat tgacagcaga
    44401 cttttctgat catgaagtca cagaagaaag tttggaaaaa caaattaacg aattatctgc
    44461 tcagcgagaa acattaaaag ccgagttggc aaaagccaaa gagcagcgag atagaaggca
    44521 aaaggagatc gataagcttg aagcagtctt ggcagagcgt aatcgtgagc aaaaagcacg
    44581 actgtcggaa caatccaaat tagaagtcca aaaagaccga gctgaaatga ttttagataa
    44641 tcatttgacc tatctgcagt cagaatatca gctgacattt gagaaagctt cccaagacta
    44701 tcaagaaaca accgatatcg aagacagtag aacaaaggtt tcatccttaa aagaacagat
    44761 tgaaaaactg ggaccagtaa acttaaattc aatcgaacaa tacgaacaag tcagtgaaag
    44821 acatacattc ttagcaacac agcgagatga tctgttagca gcgaaaaacc aattgtttga
    44881 aacgatggat gagatggatg atgaagtgcg gacaagattc aaagaagtat ttgaagctat
    44941 ccgtcaagaa ttcaaaattg tctttccaaa tatgtttggc ggcggtaaag ctgaattagt
    45001 actgacggaa ccaactgatt tgctgaaaac aggtattgag attgaagtac aacctcctgg
    45061 caaaaaactg caaagtctaa gtttgctttc tggaggagaa cgagcactta cagccatcgc
    45121 gttgctcttt tccattatcc gtgtctgtcc tgttccgttt tgtatcttag atgaggtaga
    45181 agcagcgtta gatgaagcca acgtgaaacg ttttggtcgt tacttgagcg atttccaaga
    45241 tgacacacaa tttatcgtag tcacacaccg caaaggaacg atggtcgcag cagatgtact
    45301 gtacggagtg acgatgcagg aatccggtgt atcaaaaatc gtttctgtac ggatggaaga
    45361 tatcaatgaa gaggcgaaaa tcaacgaaac gaaagtgtag gaacgaaaat gatcaaatta
    45421 gtagcaatcg atttggacgg tacattatta aatgaacaaa agctgatttc agatgagaat
    45481 aaacaagcat tagcacaagc aaaacagcaa ggggtcaaga tcgttctatg tacaggacgc
    45541 cccttagctg cgatggcgca ttatctgcaa gaattaggac tggtagatga aggtgactac
    45601 agcatcactt tcaacggcgg tttggtacaa aaaaatgata ctggtgaaat catggaaaag
    45661 aaagtgatgg aagtatcaga tatccaacgc ttatacaaac tggctcagca attggatctg
    45721 ccactagatg ttttgtctga caatgtggtt ttacagctgc ctagtgcccc aaggaagcaa
    45781 tccttgtaca atgtgttgaa taagctgctg caattccaac cagcggatat aagcgatatt
    45841 acagatgaat ttgtgcttaa taaggcagta atcgcttatg accaagaaga attagatcct
    45901 aaaatcaaag aaataccagc agaattccat gaaatgtatg agatcatcaa aacaagaagt
    45961 atgcttttgg aatttatgcc aaaaggtgtt acaaaagctt atggtatttc tttattggca
    46021 aaagatcttg gtttgaaaca agaagaaatc atggcgatcg gtgatgagga aaatgattta
    46081 ccgatgatcc aatatgctgg actaggcgta gcgatggaga atgctgtgcc tttcgtcaaa
    46141 gaagcagcag atttcgtgac agccagcaat gaagaaaatg gcgtagccaa agtgatccaa
    46201 aaatttgtat tagagtaggg ggagtcaaat gggatttttt gataaaatca agcgtgcttt
    46261 tactggcgaa gagaaaacag aagaagttga agtacaggag aagtacgaca agggtttaga
    46321 aaagacgcgt aaaacatttg gtgaacggat gaatgagttg tttgccaatt ttcgaacagt
    46381 cgatgaagac ttttttgaag aattagaaga aacacttatc ggtgcagatg taggttttga
    46441 aacagcgata cgaatcaccg aagcattacg tcaagaagtg aaactcagaa atgcgaaaaa
    46501 atcggcagaa gtccaaaatg cgatcatcga gaaacttgtg gatctctatg aagcggaagg
    46561 aatcaatgaa gtaaatgaac taaatatcca agaagacgga ctaacagtta ttttgtttgt
    46621 cggtgttaat ggtgtaggga aaacaacaag tattggtaaa cttgcccatc agttcaaaaa
    46681 cgaaggtaaa aaagtattga tggcagcagc agataccttc cgcgctggag caatcgacca
    46741 gttagttgtc tggggcgaac gcgctggtgt tgaagttgtt cgtggcaatg caggaggcga
    46801 tccagcagct gtcgtattcg atgctttgga aagagcaaag acagagcagg cagatgtttt
    46861 actagttgat actgcaggcc gactgcaaaa taaagtgaac ttgatgaacg aattagaaaa
    46921 aataaaacga gtaatccagc gggaattacc agaagcaccc caagaaacac ttcttgtatt
    46981 agatgcaacc actggtcaaa acgcgatggt tcaagcaaaa caattcaaag aaacaacaga
    47041 tgtgaccggt cttgttttga caaaattaga tggtacagca aagggcggta tcgtacttgc
    47101 tatccgtaat gaattgcatt taccagtgaa attggtcggt cttggtgaag gaatcgatga
    47161 cttggagccg tttgatccaa atgattttgt cgttggattg ttcaaaggat tactaaaaga
    47221 agaataaccc atgtctaaaa agcaggagct gtgacaaaag ttgcagctcc ttgtttgaat
    47281 gattgttttt aacatagctg tgtttttgat gccttcattt ttatacttat ttgtaagcgg
    47341 ttctcaaaca atttcgttct ttttcttaga ctttttgatt ataattagat tcatatgaat
    47401 tggaggtcac ttaatgatga attttaagca attattattg catgtcaatg cgcgcccttt
    47461 tatcgaccaa gctcgatttg gtatagaacg ggaaggacag cgggttgatc ttgcaggaaa
    47521 tctagcaaaa accgatcatc cagcaatctt tggcgatcga tcctatcacc cctatatcca
    47581 aacagatttt agcgaaacac aaacagagat gatcacccct gttaccgatt ctattcccga
    47641 attatttcag tatctcgctg ctgtttatga tgtgactgct cgttctatac cgaaagaaga
    47701 gatgatctgg ccattaagta tgccacctgc cttaccggaa aaagacgaag agatcattat
    47761 tgcaaaatta aaaaatttcg aagatgtctt gtatcgacgt tatttagcaa aagaatacgg
    47821 gaagcgaaaa cagatggtaa gcggtattca tttcaatttt gaatttggcg atgagttgct
    47881 aagaacattg ttcagccatc aggaagaatt tcaagatttt tccgaattca aaacagaact
    47941 ttatttgaaa acagcgagaa actttatgcg ctatcgctgg atgatcactt atttattcgg
    48001 tgcttcacca atgagtgaga agaactactt tttagatgaa tcacatccgc aagaacctgt
    48061 tcgcagtatt cgcaacagtg cgctgggcta tacgaatcat ccaaatgtga aagtttccta
    48121 tgcttctatg aaacagtatt tagcagatat cgagcgaatg atcgaagaag ggaaactttc
    48181 ggaggagaaa gaattttata caccgcttcg tttccgaggc ggaaagaaag tcgcagattt
    48241 agcaacaaca ggtgttcgtt atattgaatt gcggaatatc gatttgaatc cttatgcaag
    48301 attgggtatc aatccagagc aagttcggtt cttacaatta ttcctgatgt acatgttatg
    48361 gacagaagaa aaagaagact gtgaccaatg ggtagcagaa ggtacgactc gaaataacaa
    48421 agtagcacta gaacaaccat cagatcaaac agaatttcat caagaaggta gagaaattct
    48481 tgaaggaatg aagcaaatgc tggttgaatt ggattggttg gattctcttt atttagtaga
    48541 ggaagcattg actcaaatgg atcatcctga acaaacgtta gcagcgaaac tctatcaaga
    48601 agcacagcta tcgagtcagc aagaagttgc tgtggcattg ggacatcaat attataaaga
    48661 aagccatgaa cgtccctatc agttggccgg ttttcgcgaa atggaacttt ctactcagat
    48721 atttatgttc gatgccatcc aaaaaggtgt ccaagtcaaa gtattagatg agtcggatca
    48781 atttttgcgt ctgcaattcc aagaccatgt agaatatgtg aaaaatgcaa acatgacaag
    48841 taaggacagc tatatcgtcc cacttatcat ggaaaacaaa acagtaacga aaaaagtctt
    48901 aaaagaagct gggttccggg taccaggagg cgcagaattt tcatccatgg aagaagcggt
    48961 aaaagcttat ccgagatttg ccgagcaggc atttgtgatc aaacctaaat caaccaatta
    49021 cgggttaggt atcacgattt tcaaggaagg agctagtctg gaagattatc aagcaggttt
    49081 agccattgct tttcgtgaag acagttcggt tttagtagaa gaatttatgc cgggaacgga
    49141 atatcgtttc tttgtgatcg acggagaagt ccaagccatc atgttgcgag tccctgctaa
    49201 tgttattggt gacagtatcc gaacagtgaa agaacttgtt gaagaaaaaa atagtgaccc
    49261 tttgcgtgga acgaatcatc gtgcaccttt agaattgatc caattaggtg aattagaaca
    49321 attgatgttg aaagaacaag ggttgacgat cgaatcagtt ccccaagcca atcaaattgt
    49381 ttatctaagg gagaactcaa atatcagtac aggtggtgat tcgatcgata tgactgatga
    49441 attttctgaa gcttacaaaa aaatcgccgt ttctgcggta gaagcactag gagctaagat
    49501 tagtggtatc gatttgatta taccagataa agaaatcgat cctacaactg ataaaaaagc
    49561 atatgggatc atcgaagcaa atttcaatcc agccatgcat atgcatgttt atccttttgc
    49621 aggaaaaggc agaagattga cgatgaatgt attgaaattg ttatatcctg aagtatttta
    49681 aaagaaaaag cttttgacag attgtcctat ttgatgtcaa tctgatcaaa agcttttttt
    49741 gcatgtaaaa atttatgtgt tttttcgttt taaaataaac gatcccagaa gctttttttc
    49801 ttttttggtt tttctttttc agctggcgaa gagttttctt ctgggtattt tttttcgata
    49861 tcaatcacag gttcatttac tgcttctttt gctgttaaga gcaatccgaa ttcctcagga
    49921 tttgcagtga cgtaatcgtt gactacagtg aaacggctat tgtttttcgt tgctagctgg
    49981 atatattggt tttggacaga aaggggtaga gccccattca agagtatgga agcatctgga
    50041 tgttttttga gttcttttaa aaagttttct ttgttttttg gttggatcat ttcagcaaca
    50101 gtcatactaa gatagcagcg ttccctgaat gtccctaaat atttatgctg ttcttcaggg
    50161 ttcactaaag gtgtaccata aagtccctta tccaaatggc tttttacatc atcttgtgcc
    50221 ataaaaaata ccctccttct tctctttgtc tttcttacga ttatagcata ttcccaataa
    50281 tacctaaaac atatgaaaaa gcggttgctt ttgttcaacg attcacttaa aataagaatg
    50341 aaaatcattc tcaattagaa aggtggattg gatgcgctac ggacgactag tagcattatt
    50401 agttatttta agtagtattt cattgttcat cggagtatcg aatgtacaat tgtcagaact
    50461 gctccagtta gatgggcaaa gcttacaaat cttgctagtg actcgtttgc cacgaacgat
    50521 ttgtttgatc cttgtgggag cgaccagtag tatttgtggg ttgatcatgc aacatttgac
    50581 ccaaaataaa tttgtttcac caacgactgc gggcacgatg gatagtgcgc gtttaggtat
    50641 tttagtggct atgattttcc tacctggtgc atccttgatt gtccgatcct tgacagcatt
    50701 ttgctttgct tttgcaggaa cattgctttt tttatccctt acgcgattgt tcctccagaa
    50761 aaatcaattg atgcttccgt tgattggagt catgttaggg aatatcattg gttcagttgc
    50821 tacttttttt gcttatcaat tccagttggt acaaaatatg acttcatggc ttcaagggaa
    50881 ttttgcgact gtgatacgag gaaattatga gttattgtat gctgttgttc cattgttgct
    50941 cttggtttgg ctatttgctt accgatttac gatcattggt ctgggcgaat cgtttgcagt
    51001 aaatctggga atagattacc agaagatgca gtttttaggc attggcttag tatcattggc
    51061 tagtgcagtc atgttgatca tggtaggaag cattcctttt atcggagtga tcattccgaa
    51121 tcttgtctct agaatatatg gagatcaagt gcacaaaaca attggcgtaa ctgctttagc
    51181 aggtagtttg ttcttagtat tttgcgatct tgtcgctcgc gtgttgattt ttccttatga
    51241 aataccagtc agtgtgatcg ttggaatcat cggtggggct atcttccttt atcttctgat
    51301 aaggggacga cgatatgctt aaaaaaaagc catctatttt tattggtatg cttgttcttg
    51361 ttttatttgt tacaggaatg tacttgacga tccatacata tggaaactgg cattttgcat
    51421 gtgcattgag gagtaagaaa atttttgcat ttatccttgt ctcgctagct gtcagcagtg
    51481 ccacaatcag ctttcagaca attacgcata accagttttt gactccttca gtattgggac
    51541 ttgagtcgtt atatgtcctt gtccaaacgg tccttttctt ctttatcgga ggagtgacga
    51601 tgcttcagca gacaggtaca gtttttttct tattgaacgt tgggctgatg gtcgggctga
    51661 gtctattgtt atttttcttt cttttgaaaa aaatgagagc gaatcttttt ttattactga
    51721 tgtcagggat gattttaggt acgctttttg gtagtgtcag taccttttta caagtcatca
    51781 tggatccgaa tgaatatgat ttgcttcaag gcagattatt tgcaagcttt ggcaatatcc
    51841 agagtcagta tcttttgcct gcttctttga tcattatcgg ctgctggtac ctgttatttc
    51901 gatatcgaca tgtgctagac gttttccatc tcggaaatga acaagcagta aacctcggga
    51961 tagacacaga tcgttttgaa tttttgattt tatgttgtgt cagtcttttg acagggacat
    52021 caacggctct tgtgggtcca gttacttttt tagggtttat tgtcgcaaat atcagttatc
    52081 gtgtcttttc tacttatcgt catggtcagc tatttatcgg aagctttctg gtagctatgc
    52141 tgtttctgac aggaggacag ctcgcagtag agcagatttt cggctggaat acaacagtga
    52201 gtgtcgtcgt tgaatttgtc ggaggaattt attttgtcac aaaattgatt cgcgaaagga
    52261 agagtatcct atgattgatt tacaaaaggt caataaactg taccatggaa aatcagtggt
    52321 taaagatgta cagttaacga ttcctgaaaa gaagttgact gcttttatcg gtccaaatgg
    52381 tgctggaaag tcaacggtgt tgtcgatgat cagtcgtttg ataccaaaag atacaggtga
    52441 aatctattta gaccataacg aagtaaaggc ttggaaatcc aatgaattag ccaaaaaatt
    52501 atcgatccta cgacaatcga atgaaataaa tgtccaaatc accgtgaaag aattagtaga
    52561 atttggacga tttccttata cgaaaggaaa attgacgaca aaagacgagg aaatcgttca
    52621 ggaatcactg gaacaccttg ggttagaaga aatagcggat caatcgattg acactctttc
    52681 aggcggtcaa ctccaacgtg cgtatattgc aatggtgcta gcgcaagaca cggattatat
    52741 tttactggat gaacctttga acaatctaga tatgaatttt gcggttcaaa tcatgcagat
    52801 tttaaaaaat ttagtacaag aaatcgggaa aacaattgtt attgttcttc atgatatcaa
    52861 ctttgctgca agttatgcag acgaaattgt tgcaatgaaa gatggacagt tatttacgca
    52921 aggaacgacc caagaaatga taaaaaaaga tgtattggat gaattatacg aaatggacat
    52981 acggatttgt gagattgaag gccgcagatt ctgtctttat ttctaaaact aacattagga
    53041 gagaagagat gaaaaaatta gtagtaggta tcttgattct agcatcattt ggtttagcag
    53101 catgtggaaa tagcaacaac accagtcagg cagacacaaa aaaaagcagc acacaaactg
    53161 aaactacatt gacgataaca gatagtaatg gagaccaaat cgaggtgcca aataatccag
    53221 aaaaagtcgt cgtattcgac aatggttctt tagatacgat ggatgctctt ggtgttggag
    53281 ataaggtagt tggagcagct acaagcagcc ttcctgagta tctttcatcc tataaaaaag
    53341 tagaatcagc aggtggaata aaagaaccag acttagaaaa aattaatcag cttcagccag
    53401 atctaatcat catttctggc cgacaaagag attttcaaaa agatctaagt caaattgctc
    53461 cgaccatttt cctttcttta gatgcgaaaa atccatggga atctttccag caaaatgtca
    53521 ctgctttagg ggagatcttt ggtaagcaag aagaagctaa aactcaatta gaggaattat
    53581 ctagtgcaat cgatcagacg aaaaagaaag cagaagctac tgacaaaaaa gcactagtca
    53641 cacttgtgaa tgaagggcaa ttatcagcct acggatctgg ttctagattc ggtttcatcc
    53701 atgatttatt tggctttgaa caagcagatg atcaaatcga agcatccact cacggtcaaa
    53761 gtgtttctta tgaatatgtg ctggaaaaaa atccggatat cttatttgta gtagacagaa
    53821 caaaagctat tggtggagac gacagcaaag atgatatttc tgccaatgaa ctggtagcac
    53881 aaacgaatgc tggtaaaaat cagcagatca tctcccttga accagatgtc tggtatttaa
    53941 gcgggggcgg tttagaatca atgaaactga tgatcgaaga tgtgaatcaa gcatttaaat
    54001 agcataaata acagaggatt aaactctcat actatgtaac aataatgttt cttacattct
    54061 taaatagcaa gagcctatcc tcaaagaagt tatcaaaaac catccgtaaa atcgtattga
    54121 tgattttacg gatggttttt atttatttga gaaatctttt ttcaacgtga attcattatc
    54181 tacatcatac gggttgattc tagaatgggg agtggcaaaa aatagtgtga tccatttttt
    54241 cagcatcaag atcgcctcct ttgatttgaa tcaaaggtac aaagcgaaac ttagataaaa
    54301 cttaaattta gatattttta ttttgtaccg atctcttctt taaacgatgt tccatctgct
    54361 gcttctcggc aataagtcca ctagactaga aaatatgaga agctattttc tgtccagcgt
    54421 tcttattact caaaacaaat cgcagttgtc acaatctctt ctaaacgatg ttccatctgc
    54481 tgcttctcgg caataagccc actagactag aaaatatgag aagctatttt ctgtccagcg
    54541 ttcttattac tcaaagcaaa tcgcagttgt cacaatctct tctaaacaat gttcaatcag
    54601 ctgaccttcg gtattagctc aaagcactga tttcacaacc tctatttagg catgtgtctc
    54661 agcaagcggt tgatagttga tgatctgcat ttttcgataa aaatcggcgg gaagcttact
    54721 ccaccgtttt agtaattgat agaaataact ctcttctgct ttgattttat actcgcttaa
    54781 tatcaatcca aaactattcg ttttttttga agctaatttt ttataaatat ccaatgcttt
    54841 ggttttatct tcattgacaa agagtgtttt tacttcaata taagaaccct cgatattttg
    54901 tgtttcttcg ataaaataga tgttttttat atattccttc atagtgattc ctccaaagaa
    54961 gtcttttatt tgataagttt actctaccga agagttgtta ataatccgtg aacaaaaagt
    55021 aattcttttt ccaatagttg ttaaaggttt tttatttttt atagtatttt ttgttattta
    55081 agaaaggaat aaggttaaac aaataacagg ttgctttttg ggtctttttc cgttcaagaa
    55141 actgaacgaa gaaaagggaa agttgtctct tttgtgataa gagaaatcat aataaacgaa
    55201 tgacttactt tccaataaat tgagaacagt cagaaccaga caaattagtt gcatctgatg
    55261 aataaagtga ttattctatg acatgaggtg atacagatga aattagaagt gacaaaagaa
    55321 gcacaagaag tattgaagaa taaattagaa gcagacgatc aacttttatt agatatcgaa
    55381 aacggtgacg gtccatttgc agaaagccaa gtcagttgcc aattggatac ggcatttcga
    55441 ttgatcattg tcaaaaaaga tacacaaaca gatctaagtc tttattctgt agtggctgat
    55501 acaccagttg gaccaatcaa aatcaaagac agtgcaatac tttacatgga tgatccatcc
    55561 acacttgctt tagaaccaac ttataatagt ttacaattaa aaggaccaag cggcttgaga
    55621 aaaggcaact tgcaagttgt tcgaaaagac tagagggata aaatgggaaa attggcgatc
    55681 atcagcgacc tacatgtgga catcaataaa ttcggcgaat cagagctgat gcagctttgg
    55741 caagttttgc aaaagcacaa gatcactcgg cttcatctag ccggagatac ggctaataaa
    55801 gtagaccgtt gcctagctgt tgtcgaattt ttcgtcaata aaggattatc tacgacattt
    55861 catttcggca atcacgagtt ggcagatgtg cccaatgaac agatgatgga ccattttcca
    55921 gacccacatt ttttaaatga acggtatatt gctttaaatg agcagaccgt tcttttaggg
    55981 atgaatggct ggtacgacta tcagttttcc gaattgaaag attacgaggc aatcgtacga
    56041 atgaagcgtt tgtattggta tgatcggttc atccagcgtg ggaaaacaga tctcgaggtc
    56101 aatgcagaaa tgcttgcagc tgcaaaaaat ttgctagacc agctggaagc aaaaaaactt
    56161 gaggtgattt tatccactca tttcgttcct aaacgggcat ttatcatgta tcaatctgct
    56221 ccttatgagc gttggaacaa attaaatgca tttttaggat cagaatcatt cggagaatta
    56281 cttgaccgtt attcaaatat caaacaagta gtttttggcc atacgcatcg tcgctttgaa
    56341 gataaatcca tcggtcacac gatttacagc tgcagacctt ttggttatta ttatgaatgg
    56401 cagctgaccc gagattttgt cttgaaagaa cagttgctag acagctatga tcctatgaaa
    56461 ttacgtgtcc tcttgcgtgc ccatcaccca gcttttcagg cctatcaaac acaacatttg
    56521 caagaggaat ttgaacaagc gatgacaatc gttccgtatt gagacagcat tagtaatgtg
    56581 taaaagaaaa taccttgtaa aacataagga taagtaagag cagtagatcc atttatcgtg
    56641 gatatgctgc tttttttgcg gaaaataatc agcgaatgtt tatttgatcg gatgatttgt
    56701 aactcgcaga gtactagatg caagcgtgta ttcttttttt gaacgataat cacaaaaaaa
    56761 aatataatat acatttttta tttaattata attaatattg attgaagtct aggcagtcaa
    56821 gtcacatttt gatagatagg agggggactt acgaattatt ttaaaggcta tgacagctgt
    56881 atttctagat tgtgtttgca tcaagttgca ataaaaacta gaaatcaaat cgggctgata
    56941 atcatttata actaatcgat acataggacc aaatataggt ctatgttctt ttatttcata
    57001 agtatcgtaa aacagaattt tgctatggat taatccaatc gactgatatg tttagtaaat
    57061 aaagaaagag gaatgtcaat gatgaaaaaa gaaatcgtat taacagcagc aatgatgtta
    57121 ttcagcatgg tagcaagtac gacttttgtt tctgcaactg aagtataccc aaaggaatat
    57181 aacacagaag gaacgattac atttgaagca ggagatgagg gtgtaactcc tcctgttgat
    57241 ccagagaatc ctgatcccaa taaacctgtc gatccatcag atcctccaag ccctggaaca
    57301 ggaggggcat tatcgattga ttatggatca aaattcaaat ttgggactca aaaaatttct
    57361 actgcggata aaacgtatta tgcagctgct gatgtgatga acgatgggtc tagaaaaccg
    57421 acatatgtcc aagtgacaga taggagaagt accttatctg ggtggaaatt aagtgtctct
    57481 caaccagaac agtttaaaac tgccagtggg gatgaactag ttggagcaca gctgaaattt
    57541 acgaaagggc aggctgtttc tttggttgat ccaacatata cgccgcaaac ggtgaattct
    57601 gaattaacgt tgacaccggg aggaaacaat acgttagcaa ttaatgctaa atcaggaaca
    57661 ggtgtgggga cttgggtcta tcgatttggt gcaaatgaaa atgaaaatca agatgcggta
    57721 cagctttcgg tacccggtaa atctgtcaag ttagcgcaac aatattccac taaattagtt
    57781 tggacattag aagatacacc taacaactag tttactgaaa agaactgtta aaaataaata
    57841 cgtagtcata ttgtcttgcg aggtaatcga gcaaaaattg tcaaaggcta ggctaaattt
    57901 gtttggatac gaatagttca tctaggaatt taacgatcag gaggaaaaga tatattgaaa
    57961 aagaggagga tcgtgctcat tttaagcagt ctcttagtga ctaatatctt tccgatcata
    58021 gggtactctt tggaattgaa tcagttggag catggaaata gaagagacga agaaagcaaa
    58081 ctcacttcga cattcgacag tttaatagaa tccaaccaga cacaagaaag cagtgaggat
    58141 ttaagtattt cgagtagtgg agagaccact gacaacgtag aagctgaggg ttcggacaaa
    58201 caaggactag aagatgcacc aatttctgcg gttgagctgc ctgacgatcc caatattatt
    58261 tctatcgata aaatttttca aacacctatc ggtactagca cgtccatatt ggaaaatggg
    58321 aagttactgc agttgaaccc agcagaaagg tctcaaagag gagctatttg gtcaaataag
    58381 aagattagtt tactctcgga ttttacattt aaatcttatt tatacttagg aaatcaacga
    58441 gatagagcgg gagatggcat gacctttaca ttgactggcg atcctagaat ggctagtaat
    58501 cccagtcaag ttattggatc gccaggtatg ggaattggct catattctac tcgttctggc
    58561 caaccttaca ttgcgaatgc gctctcgatt gagtttgata cgtataaaaa tagtggttct
    58621 tctgatcgaa tggatcgaga gataactgct gataatagag ggcatggaca tgtcgctttt
    58681 gtcacgccta aaccaaataa taataattat tctggagagc atactggcgt gacggtagct
    58741 cctacatttc tttctaatgg cacatggcgc ttgttaataa ttaaatggga cgcggagcaa
    58801 agaagtctta cttatgattt ggaaggtgtc ggtcgctctt ctcggattat acctgatctc
    58861 attaggcagt ttggttctac agaagtaaat tgggggttta cttcttcaac aggtactttt
    58921 tttcaagaaa atgctgtcgc tgtcacgcga cttccatcga atgtcacatc aacagctgat
    58981 gtaaaagtca acgatagtga ttatggggcc atcgctgaag ctggaggaaa cgataagata
    59041 actatcagaa atacattgaa ggtcgagggg gattttcttg aatataagaa aatgcagact
    59101 agtatcgacc tcccacaaga attaactatt cccgagcata tcttgatcaa tggaaatcaa
    59161 attgatgcca aagatatctt gataaccgga aatcgagtca cgctaaatct gacagattat
    59221 tttattgctg gagatacgtt aataatcgaa ttggagacaa ctttgcagga agtaattcct
    59281 gaaaaagtac tcactatgca ttttgaatac cttgaaaacg ggaatttgat tgagcaatcg
    59341 aatgaaataa acctaaagat cgcaaagctg aaggagatag atatccatgt ttattataaa
    59401 gataaagaca gtcagattga tattgctgaa atgaaaacaa tcactgggaa aataggagag
    59461 aagtataaag aaactcctat tgatattgaa gggtatgttt ttgactcgga ttcagcgaat
    59521 acagaaggca tcttctccga aaatacagag gatatctatt tttattacag aaaaggcaaa
    59581 ctttatctct caactgctcc tcaatatgtc aattttggaa aacataaaat atccagtaga
    59641 ccattaagtg aattcggaca gttttcaggt gggttgaaaa ttatagatga acgggctttt
    59701 ggaagttgga aattacaatt gaaacaagag aaagttctta caaatggaga ttttgagctt
    59761 ccagaagctc tttcctttgt tacctctgaa aatacaacgg ttatagggac atccgctgtt
    59821 acgattttcg aaagtgatca aaagggtgaa agcgatttgt cctctctttt agattctaca
    59881 gctcatcgtg gaatcagaat aaatattcct gtagaaaacc aaagaatcgg aacctttgag
    59941 gggaaactat cttggatact tgcagatgtc ccaggaaatt aacaggaaga gaagaatata
    60001 acaaaattat tgagtttcaa gaagggcaca ggtaccatag gctcttgtgt cccacagaga
    60061 caagattttg ttatagtctt ctcttacata tgattaaagg agcgattttc ttgagaccaa
    60121 aacaacaatt atttataaca ctcctagtta ttttaggggc actttttaac aatatatcag
    60181 tgtcagctag tgcatttaat ttttcagtga cgccgataac ttcagagaat caaatcgaca
    60241 agagaaaaac ttacttcgat cttcaacttg atccggatca ggaagtagaa gtaaaagcgg
    60301 aattgagaaa tgatacagaa aaagaagtga aaatcgatat ttctgtgaat agcgcaacca
    60361 ccaattcaaa tgtgatggtt gaatacggaa aaaacgagat tgaaaaagac atgagcctga
    60421 tctttgatct ggtagattat gtgagctatc cacagatagt gacgctaaaa cctaaaagtg
    60481 tccaaaccgt tgcttttcat gtcaggatgc cgaatgagcg atttgatggt gtattggctg
    60541 gtgggatcac attccaagaa atccagacgg aaaaggacca aacagaaact aaggatcaaa
    60601 gcttatcgat tgagaatgaa tacacctata ttattgcttt gttgatgcaa cagacactga
    60661 acgaagttgc tccgaacctt actcttcatg aagttaaacc agatcaaatc aatgcaagaa
    60721 atgtcatctt agcaaacgtt caaaatgatc aaaaaaccta tatcaatcaa gtagttattg
    60781 aaacaaaaat tacaaaaaaa ggacattctg aagttttata tcaagaggaa aaagaaggat
    60841 tgcagattgc gccgaataca aatttttctt ttccaactgc tttaaatggt caaccgctaa
    60901 ccccaggtga atatcacctt acaatgactg ttttaggaaa tgaaaatgaa aatggaaaat
    60961 tttcgagaaa aaaaggaaat actacgatca actataccaa tcaatgggta tttgaaaaag
    61021 attttacaat agacggaaaa gtagccaagg aattgaacac gacggatgtt acgattaaaa
    61081 aagacaataa ttggatttat atcttagtag cttcactttt cttattgttt attttattat
    61141 gcatactttt gtttgttttg agaaaaaaag aagagaaaga aaagtaaggt taagataaca
    61201 aatagtaaat ttttattatt gtaggatggt tgggctcttt ttataacaat ataaaatata
    61261 ccattttatt aataaaaatg catactagta aatgatataa ttctaataac aggtttgatc
    61321 gcgagaaagg ccaaataaaa cgctctccaa aatgttaaaa aaaagacagg acgatgtata
    61381 ttatacaaac aagaatcgct gacaacagtg actcggatcc aggaaatcag ttttatcccc
    61441 agctagttgg tgaatgcatg gaaagtaata tacgtagaaa gagctgatga ctaatgaatt
    61501 atgagttttt tttaagcaaa gaagatcaca gaagttattc attacttaaa tatttagaag
    61561 caagctcaaa tttatcagag tcaatatcga atgttcaaga agagttgtcg ctatcgacat
    61621 ttctcttgaa aaaaacgatc gataagctga aacatgattt acaaaagttc gatctagaga
    61681 aaaatttgcg agtaaccgtc agtgaattgg atatatgtct ggaaattaat ggaaaatgtt
    61741 ctagcaaagc tctgttatct cgttatataa cggattcttt atctatgaaa atggtattgg
    61801 ctttctttca ggaaaaatat atctcagtag aaaattttgc taaacagcat cacgtgagtt
    61861 attctgtggc ttataaagta ttacaaagac taaaaagaaa tctaaaaaaa tatcaaattt
    61921 tttttgaaaa gagaaagtt
//
LOCUS       DACEOM010000007        58770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEOM010000007 DACEOM010000000
VERSION     DACEOM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.007"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(56..796)
                     /locus_tag="IW781_000416"
     CDS             complement(56..796)
                     /locus_tag="IW781_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181436.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(926..1873)
                     /locus_tag="IW781_000417"
     CDS             complement(926..1873)
                     /locus_tag="IW781_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1181437.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2002..2766)
                     /locus_tag="IW781_000418"
     CDS             complement(2002..2766)
                     /locus_tag="IW781_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1181438.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2770..3159)
                     /locus_tag="IW781_000419"
     CDS             complement(2770..3159)
                     /locus_tag="IW781_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181439.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3294..3836
                     /locus_tag="IW781_000420"
     CDS             3294..3836
                     /locus_tag="IW781_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1181440.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            3984..4616
                     /locus_tag="IW781_000421"
     CDS             3984..4616
                     /locus_tag="IW781_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181441.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4750..4875)
                     /locus_tag="IW781_000422"
     CDS             complement(4750..4875)
                     /locus_tag="IW781_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1181442.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5009..5902)
                     /locus_tag="IW781_000423"
     CDS             complement(5009..5902)
                     /locus_tag="IW781_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1181443.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6084..7010)
                     /locus_tag="IW781_000424"
     CDS             complement(6084..7010)
                     /locus_tag="IW781_000424"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1181444.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7095..7856)
                     /gene="pflA"
                     /locus_tag="IW781_000425"
     CDS             complement(7095..7856)
                     /gene="pflA"
                     /locus_tag="IW781_000425"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1181445.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8100..10331)
                     /gene="pflB"
                     /locus_tag="IW781_000426"
     CDS             complement(8100..10331)
                     /gene="pflB"
                     /locus_tag="IW781_000426"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1181446.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10608..13058)
                     /gene="parC"
                     /locus_tag="IW781_000427"
     CDS             complement(10608..13058)
                     /gene="parC"
                     /locus_tag="IW781_000427"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1181447.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13070..15115)
                     /gene="parE"
                     /locus_tag="IW781_000428"
     CDS             complement(13070..15115)
                     /gene="parE"
                     /locus_tag="IW781_000428"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1181448.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15281..15715)
                     /locus_tag="IW781_000429"
     CDS             complement(15281..15715)
                     /locus_tag="IW781_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1181449.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15842..16480
                     /gene="plsY"
                     /locus_tag="IW781_000430"
     CDS             15842..16480
                     /gene="plsY"
                     /locus_tag="IW781_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1181450.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16612..17487)
                     /locus_tag="IW781_000431"
     CDS             complement(16612..17487)
                     /locus_tag="IW781_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1181451.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17569..18360)
                     /gene="codY"
                     /locus_tag="IW781_000432"
     CDS             complement(17569..18360)
                     /gene="codY"
                     /locus_tag="IW781_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1181452.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18378..19778)
                     /gene="hslU"
                     /locus_tag="IW781_000433"
     CDS             complement(18378..19778)
                     /gene="hslU"
                     /locus_tag="IW781_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1181453.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19792..20340)
                     /gene="hslV"
                     /locus_tag="IW781_000434"
     CDS             complement(19792..20340)
                     /gene="hslV"
                     /locus_tag="IW781_000434"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1181454.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20377..21282)
                     /gene="xerC"
                     /locus_tag="IW781_000435"
     CDS             complement(20377..21282)
                     /gene="xerC"
                     /locus_tag="IW781_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1181455.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21480..22856)
                     /gene="trmFO"
                     /locus_tag="IW781_000436"
     CDS             complement(21480..22856)
                     /gene="trmFO"
                     /locus_tag="IW781_000436"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1181456.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22825..24903)
                     /gene="topA"
                     /locus_tag="IW781_000437"
     CDS             complement(22825..24903)
                     /gene="topA"
                     /locus_tag="IW781_000437"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1181457.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25025..25882)
                     /gene="dprA"
                     /locus_tag="IW781_000438"
     CDS             complement(25025..25882)
                     /gene="dprA"
                     /locus_tag="IW781_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1181458.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(25938..26705)
                     /locus_tag="IW781_000439"
     CDS             complement(25938..26705)
                     /locus_tag="IW781_000439"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1181459.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26698..27558)
                     /gene="ylqF"
                     /locus_tag="IW781_000440"
     CDS             complement(26698..27558)
                     /gene="ylqF"
                     /locus_tag="IW781_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1181460.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27818..28216
                     /locus_tag="IW781_000441"
     CDS             27818..28216
                     /locus_tag="IW781_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1181461.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28453..28995
                     /locus_tag="IW781_000442"
     CDS             28453..28995
                     /locus_tag="IW781_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1181462.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29078..29629)
                     /gene="lepB"
                     /locus_tag="IW781_000443"
     CDS             complement(29078..29629)
                     /gene="lepB"
                     /locus_tag="IW781_000443"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1181463.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29781..30008)
                     /locus_tag="IW781_000444"
     CDS             complement(29781..30008)
                     /locus_tag="IW781_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1181464.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30005..30523)
                     /gene="msrA"
                     /locus_tag="IW781_000445"
     CDS             complement(30005..30523)
                     /gene="msrA"
                     /locus_tag="IW781_000445"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1181465.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30543..31256)
                     /locus_tag="IW781_000446"
     CDS             complement(30543..31256)
                     /locus_tag="IW781_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1181466.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31259..32155)
                     /locus_tag="IW781_000447"
     CDS             complement(31259..32155)
                     /locus_tag="IW781_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1181467.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32313..33155)
                     /locus_tag="IW781_000448"
     CDS             complement(32313..33155)
                     /locus_tag="IW781_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1181468.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33287..33940
                     /locus_tag="IW781_000449"
     CDS             33287..33940
                     /locus_tag="IW781_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1181469.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34003..34530)
                     /locus_tag="IW781_000450"
     CDS             complement(34003..34530)
                     /locus_tag="IW781_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1181470.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34550..35497)
                     /locus_tag="IW781_000451"
     CDS             complement(34550..35497)
                     /locus_tag="IW781_000451"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1181471.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35509..37395)
                     /locus_tag="IW781_000452"
     CDS             complement(35509..37395)
                     /locus_tag="IW781_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1181472.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(37555..39633)
                     /locus_tag="IW781_000453"
     CDS             complement(37555..39633)
                     /locus_tag="IW781_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1181473.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39646..39921)
                     /locus_tag="IW781_000454"
     CDS             complement(39646..39921)
                     /locus_tag="IW781_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181474.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40283..40738)
                     /locus_tag="IW781_000455"
     CDS             complement(40283..40738)
                     /locus_tag="IW781_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181475.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(40892..42100)
                     /locus_tag="IW781_000456"
     CDS             complement(40892..42100)
                     /locus_tag="IW781_000456"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1181476.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42212..42544)
                     /locus_tag="IW781_000457"
     CDS             complement(42212..42544)
                     /locus_tag="IW781_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1181477.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42692..43567
                     /locus_tag="IW781_000458"
     CDS             42692..43567
                     /locus_tag="IW781_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1181478.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43649..44230)
                     /locus_tag="IW781_000459"
     CDS             complement(43649..44230)
                     /locus_tag="IW781_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1181479.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44236..45495)
                     /locus_tag="IW781_000460"
     CDS             complement(44236..45495)
                     /locus_tag="IW781_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1181480.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45497..46543)
                     /locus_tag="IW781_000461"
     CDS             complement(45497..46543)
                     /locus_tag="IW781_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181481.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46778..47053)
                     /locus_tag="IW781_000462"
     CDS             complement(46778..47053)
                     /locus_tag="IW781_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1181482.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47296..48606)
                     /locus_tag="IW781_000463"
     CDS             complement(47296..48606)
                     /locus_tag="IW781_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1181483.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48788..50017)
                     /gene="rpsA"
                     /locus_tag="IW781_000464"
     CDS             complement(48788..50017)
                     /gene="rpsA"
                     /locus_tag="IW781_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1181484.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50152..50832)
                     /locus_tag="IW781_000465"
     CDS             complement(50152..50832)
                     /locus_tag="IW781_000465"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1181485.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(50881..51516)
                     /locus_tag="IW781_000466"
     CDS             complement(50881..51516)
                     /locus_tag="IW781_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1181486.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTLTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51582..52985)
                     /locus_tag="IW781_000467"
     CDS             complement(51582..52985)
                     /locus_tag="IW781_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1181487.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(52972..54003)
                     /locus_tag="IW781_000468"
     CDS             complement(52972..54003)
                     /locus_tag="IW781_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181488.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54034..54255
                     /locus_tag="IW781_000469"
     CDS             54034..54255
                     /locus_tag="IW781_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1181489.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54420..55157)
                     /locus_tag="IW781_000470"
     CDS             complement(54420..55157)
                     /locus_tag="IW781_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1181490.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55310..56650)
                     /locus_tag="IW781_000471"
     CDS             complement(55310..56650)
                     /locus_tag="IW781_000471"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1181491.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(56887..58410)
                     /locus_tag="IW781_000472"
     CDS             complement(56887..58410)
                     /locus_tag="IW781_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1181492.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT        16116 a        12373 c        10001 g        20280 t
ORIGIN      
        1 gaaacgccgc agcgtttcct ttccaactgt agccgaatat aaatttgtat atcacttatt
       61 taaaatagct attttctccc aaagagtttc cttgatcatc gatcaatcct tgtaatgagc
      121 gttctaagat accatggctt tttttcgatt ttttttcttc tgactgcttt gtttcctcta
      181 acgtttttgt tcgattgaat gttcgtacag acggctcacc atttcttttt tcttgatacg
      241 gcgtcggttc tgtatcgaac aacagataag agtcatccgt ttttttcatc gattcgatta
      301 aatgatcctc agaaattttg ctttctctag ggtctgggat aatcgatgcg ggtacatatt
      361 taggaacaaa ataagaacgc tcccgcacct tcgtgtcaac agatgcgttt cgtgtagtat
      421 gcttggactc tgtccttgtt tcactttgtc tgacagaacg agaggaatca ccaaaaagtt
      481 tttttcgtcc tgtcgatgtg atttctgatt ggtaatggcc ggtataatcc ggtaattttc
      541 ctttatgctt ttccaaattt tccttacgcg gagctgactt caatccattt gttgcacgac
      601 gcttgcgacg gattttctcg gtgttcgtgt tcgtgaaatg aaattctttc atctgttcaa
      661 acggggactg attctgttca gtcaaaagat cttcttgtcc gtcaaataat actcgcttct
      721 tgtcgttggt taattttacg cctaactcat catcgtacgc aggaaaacgg aaatgttttc
      781 gtctgatttc tttcattcaa accatccttc tcaaaaatgg aacttttctc aataatagca
      841 cagattcatt gttttcagta gcaatgcgct gattatttta agaaaatagc gatgaatttc
      901 ataaatatat atatagtaat aagacttaat tcgttgtctc tctgataact agttcaggaa
      961 aaaaacgacg ataaccttga ctcgcccctt tttcttctat tttttttaac aacatttctc
     1021 cacatacttc acccatacgt ataatatctt gcttgatcgt tgtcaattta ggtgaactga
     1081 tttggtctaa aaatacccca tcaaatccaa tcacaccaaa ttcttcgggc aagtgtcctc
     1141 cagcatttat aatccctctt actgctccta gtgctagccg atctgaacta catacaaaac
     1201 aagttttatc tgtaaaatct gcccagtttt ctgtcatata tctttctact tctgtcgaat
     1261 gatttggaaa ccgcaagatt ttactaggaa gatcaacttc cttcatcaca gataaataac
     1321 cagtttccct tgaaatttca aaaggctcgt ccacttcgat accgatataa ataatcgttt
     1381 gataatctct ttctaaggca tattgcgttg cttttgctgt accatattca ttatcgctgt
     1441 ctacataatc atatccttca gaattttccc caaacaagac aactggtttt tccaaacggt
     1501 cgatccaatc gaagtctttt tttctgacac ctgtgatgat gtatccatca caagaaccga
     1561 cgtcgaaggc atcatttgta actaattgga gagaataatg cgctttaccg atccgtttag
     1621 cgattcccat cagcaaattc atgtaatagg gctcagttgt gtcgatttct tccagtataa
     1681 ataatttgat gatctgcgac cgattcgtga caagtgcttt tgcagcaata tttggacggt
     1741 aatccagttc agccattgct gagaaaacta actctttcaa ttcgtcagtc acctgttccg
     1801 gatgattgat gactcgagag accgtcattt ttgaaacatt ggcacgtttt gcaacatctg
     1861 ctaaagtggg catgctctct tcctttctaa catgttgtca ttcttactat caattatacc
     1921 aaattttcct aacagagttt attatagccc aaaataaaaa acggaaaatc ctctatcgtc
     1981 atgagtgatt ttccgtttca attatttata cttttttatc aaattttttg ctaaaaaagt
     2041 ggaagcttct tccattagtg gtatttgtac catatgtcgc tgtgtttttg aggaacgaaa
     2101 aacaatttct tttccgtaag acttatgccg atttttttct acaaatcgtt ccgtttgtat
     2161 ataagggatt ttttcgtcag cttgtccatg ccaaaagaac agaggacgtc cagctaactt
     2221 ttccggctgc aatgacaaat catagctctt aacccatgaa atcaaatcaa aatagtcttt
     2281 tggtacgaca taattatatt ccgatgcgcg ccttacgatt tcttttccat attccaaagg
     2341 tgcaggggaa cccatcaaac acacgcccgc tactatatga ggatgtttag ccaacaaggc
     2401 gcatgttgtc attcccccca tcgaaacccc gccaactgca agctgatcat gtagaagacc
     2461 tcttttttct aaatgcttga taagtgtgtc aaattcaaac agatttccgt agatactatc
     2521 ccaaaatgta taggatggaa tagaagaaac cggctgtttg cgctccccat gattcatcgc
     2581 atctggcagg atcacacgaa tgccctgctg cgcaattttt cttccttgcg tcaaaactaa
     2641 ctccttactt gtctgccatc cgtgataata gatgacaacg ggaatacgtt cctctcttaa
     2701 ttctttcaga acaacttcta acactggaat atgatggaca gaacgtttca atataattgg
     2761 attcaattgt tactcaccta ttttcattaa atttcctgat cgccatagca accatgctcc
     2821 aacagttgtc tggaacaggt tcagtgtacc aaataatata aatgcaggac tgaatggctc
     2881 gggaaataag cacagagaac aaagtcccac cactcctaat gctagcgaaa ggagtggata
     2941 aatagaaaaa ttcaaaaaac tggcaacgct accgaaaata ttaccaataa acagtatcaa
     3001 aaagaaaaaa acgcttaaaa ttaaaccatc tgtcagcgac atctgaaaaa caatgttctc
     3061 tacctcaatt ggctgttgaa tcgaatagac acctaagtat atactgagaa ggagacttag
     3121 cacgtggctg gcacaaaaca cacgattgac cgttttcatt ttaatcacct aacctatctt
     3181 tataattcta ttataatcat tttatcattt ttttgtatgt ataaagtttc aatttttctt
     3241 cacttacgat tttttttgat attgttgtta tataaatgaa aggagtttta caaatgattg
     3301 atccaaaaat gttggggatg atcttcctca ttaattttgc ttatatcact ctgaacacgt
     3361 tacgtttcat gttgacaatg aaaggctatc gactgattgc tccacttgtc agtatggtcg
     3421 agatcacgat ttatatcgtc ggcttatccc ttgttttaga tcgtttagat aatccactga
     3481 atttatttgt ctatgcgtta ggttacgcag taggtatcag tgtcggaatt ttgattgaag
     3541 acaaattggc attaggatac atcatggtca caacgatttt gccagcaaat agcgcagaag
     3601 ataaaagttt gccgggtata ctgagagaac acggttatgg tgtgacacag acttctgcaa
     3661 tggggctgga aggtgagcgg ctagttctcg aaatactttc tcctagaaaa agcgaacgtg
     3721 aactgtatcg cctgatcaaa gaaatcgaag agcgtgcttt tatcatttct tatgagccaa
     3781 aatatatttc aggtggtttc tggactaaaa aagtgagaaa acgacaagga aaataactca
     3841 caaacttaaa aactgtctaa tatctaaatt aaattttaaa tgttagacag tttttattta
     3901 ttattttgat taaaaaacat cgacatatac tcatcaattt gttattatac tttatatact
     3961 tatcaattta atttggagat attatggaaa ttcaacgtag aaccactaat tttttacatt
     4021 taggctcttt ttttgcaact ttgacgatct tattttttct atgttattca taccttaaga
     4081 cctcacatgc gccttctagt gcattaggct ggttgctttt ggatcattct gatatcacag
     4141 aagccgaaga aaatccgttt tttttgattt tgtcttctct ttgcggctta ctgttttctg
     4201 tttatttcgg tgcctatttt tttcatcgac atcctggtac gttaaacgaa gcttcaagcg
     4261 tattgattgc atttaaaacg ttttcttttt tagcaattct tttttattcg tttaatcaca
     4321 atacgctttt cttctttatc atgaatggtt tagtcgtgat tttatccatt tttacttatt
     4381 ttattttttc actcattttt agccaattga aatgtccgct cttctttcga ttagttcctg
     4441 tcacctgttt tatttacagc ttgttttcct tttttgtcct tgctttaatg ccactgacca
     4501 acccttcgac gatccaagtc gcaggaaatc tgttggatat gctcttcatc gtcagtctag
     4561 gtgtatttat gtggcaaaga aaaaacaaac aaaataaaaa gcattttgaa gcctgactat
     4621 tacgacttca taaatgcttt tttagtttta aacacttcaa tctaaaagaa taacccaaga
     4681 ttggataaat tattaatgac tcgccattcg tttcttagtg attctggatt atcaaatgga
     4741 cttcctaatc taatcagcta gttcttccca taaaatgcca tacttgactg aatcataata
     4801 ttgattattt aaaaatctaa cttttcttat cactacttcc tttgtcattc cacatttctc
     4861 accatttttt tgcattcctt tgtttccaga ccatgtagtg aatctaatag gtggtagata
     4921 ggcaaaatac tcaaataatt cttttaacca taaaggaaga gaaaggctcc tcctgcaaga
     4981 gtcagtataa tttttgctta ctgctctctt aggttttact cactaaaact gttccatatt
     5041 ctttgatata agaaaatgca tgcggtataa atttcttgct cagctcagaa aagatttgtc
     5101 gtttaagaaa ggccatttcc tccattgtca atgaaggatc caatgttggt aattcaatct
     5161 caaaattgtt tttttcgaca ataggtaatt ctaatgtgaa tgattcttct tcctttatag
     5221 tattcgtcac aagtagagac tgatggaaaa tactaggccg gcgattttta tatttattct
     5281 tacgtatcct gtcaacgata acttctattt gatcaaaaaa gtacgattca tttacgtctc
     5341 caataagttc ggctaattct ttgaatgcct tcgttacagg aagctgattc ttctgtacaa
     5401 aaaagtaatt agccaaagta agcgggaagt gttctataga ggaaagtgtg ataaaacggt
     5461 acttctcatt tcctgcagtt gtcctcagca attctgtctc tcggctgttt cttactccat
     5521 aaaaatacgc ttctttccgt tcatcttcga aacaatacgc ccaaataact tctcccatcg
     5581 ctaatttttg ttcatcaaca gagagtctct tcaattgttc agctatggtt tcagcagccg
     5641 aagtggcttg ttgcaagtaa tctttaggat caaatattgg aaagttcaaa tgatatcttc
     5701 gttcttccct cttgattaat cctgcatcga tcaataaatc aagaaaatgg gaaaatttat
     5761 tttgcgaaaa ttcttttttc aattctctta gaatcgtttc ttgttctcga tgttcatata
     5821 gataatcggt caatgaagaa aaaagcggat catcggtcag tttttgataa aagttttcct
     5881 tattttttga ataatagttc atatatgttc tcttttctat ccgttttatt ttacttaaaa
     5941 ggcttttacg taaaaaggag gaagaacaag agtcataatt gactgttatt ctcctccatt
     6001 tcttcggcct ttagtatgtt ctatgtttct ttttatatca atcgcttatc cattcatttt
     6061 gaatgcttca tactaccatt tgattagcta aatacagccg tcaattgagg tacgacttgt
     6121 tttttacgtg aaacgacacc tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa
     6181 gcttcttcta ctttttcttt tcctttacca gcaacaagta attctgaatc actgtctaag
     6241 atgtttgtga caactaatac gaacagatcg tagccatttg ctttcatttc atcttccatt
     6301 gctgcttcta gctctgcttg tcgatccatc acttcattaa ggtccactgt attgacttga
     6361 gcgatacgaa cgttggaact gcccattggg aaactttttg catccaaatc aagaagaacc
     6421 gcagctgatt tatcacttag atttgttcct gctttcaaca tttcaagtcc ataagtgttt
     6481 agatcgactt cagcgattgc tgcaagttct tttgcagctg cttcatcttc aggcgtacat
     6541 gttggtgatt tgaacaacaa tgtatctgaa atgatagctg agagcatgat tcctgcgatt
     6601 tctttaggaa ttgttacttg gttttcttta tatagtttta aaataatcgt acttgtacat
     6661 ccaactggtt ctgcacgata gtacaatgga ttagctgttt caaagtttgc aatacgatgg
     6721 tggtctacta cagctaaaat atttacttcg ctgatatctg aaatactttg ttggaattca
     6781 ttatgatcca caagcatgac atctgcattt tctgctttag ctgactctac tactcgtggt
     6841 gcagatagct gaaagtggtc taatgcgtat tgtgtttctt cattaggtgt acctagagca
     6901 actgcttctg tttctttacc taattcattt tgcaagtgtg aaaacgcaat ggctgccccg
     6961 attgcatctg tatctggatt ttggtgacca aaaactaaaa tttttgacat gtcattcgct
     7021 cctttgtgat ttctcctatt taataatagc aaaaaaacgg aaagaagcaa tacttatctt
     7081 ctttccgatg cgacctatcg cgttaaatat tttgtatact cttctgtatg aagcaatcgt
     7141 ttagcatttt cgacacgttc ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa
     7201 ccaagtgttt cccacttgta ttttcccatc gtatgataag gaagaatctc tactctgtct
     7261 acatttgaca aggtttgaat aaagttgtct agtcggatca aatattcatc ataatcactt
     7321 cgctcaggaa ctagtacatg gcgaatccaa accggtttgt tgatttttga caaatattga
     7381 gccatttcta aaatattttc attgtcccaa tgcgttagtt ttttatgctc ttcattatcg
     7441 atgtgtttga tgtcaaataa cagtaaatct gtatacttca tcaattcttg aaaacgtgaa
     7501 aagaaaggtt cttcatacgt aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc
     7561 cgctcttttg cttttttaaa tagttcgatc aaaaaatcga tctgcaatag aggttctcca
     7621 ccactaacag taataccacc tttatctccc cagtaaggtc tgaagcgttc tgctttatct
     7681 aaaagttcat ctgctgtgta ttctttccct ccgccaatat tccaagtatc tggattgtga
     7741 caaaattggc aacgcatgcg gcatccttgc ataaacacga caaagcgcag tccaggtccg
     7801 tctactgaac cgaaagtttc gatagaatga acatagccga ttgttttttc ttccatttaa
     7861 gttcacatct tttctttgat ttcattttaa gtacaagtta gctgtcgaag cgaagctttc
     7921 tgaacaaaat ttttcgcaat tgccgcttcg ttatgtgtaa aatttacagg gacggaacat
     7981 acaagtcctg cccctgtaaa tcgtttcttt caaataagca tactgattat tcgattttga
     8041 tgcgatggct attcaacgtc ctttcgacgc taagtcgaaa aacttttttc acgaactttt
     8101 tacattctgt cgtgtgaagt tctcatcaag acatctaatt gttgttctct tgtcaagtca
     8161 cggaatttaa ctgcataacc agatacccgg atcgtcaagt tcggatattt cattggattt
     8221 tccattgcat cacgcaataa atcattactg aatacattga cattcaaatg ataagctcct
     8281 tttgagaagt atccatctaa gacattacgc aagttttcga ttcttgtatc ttgttctttt
     8341 ccaagacctg aaggattgat tgtttgggta tttgaaattc catccaatgc acagccgtaa
     8401 ttcattttag ctgttgagtt caggctagct aataacccac tttgttccgc attgtaactt
     8461 ggatttgctc ctggcgcaag tggttttcca gcttcacggc catcaggagt atttcctgta
     8521 tttttaccgt aaacaacatt tgaagtaatc gtcaataatg aagttgttgg ttttgagttg
     8581 cggtaagttt tatgacgttt gatttgtccc atgaagtatt ccaagatcca tgaaactaat
     8641 tcatctgcac ggtcatcatc atttccgtat ttagggaagt cgccatccac ttcaaaatca
     8701 actgctattc ctttttcatt acggattgct tttacttttg catatttgat cgctgacaag
     8761 ctgtctgccg catgagagat cccagcaata cctgtagcaa atgtgcgttg caagtcagaa
     8821 tccatcaaag ccagctgagc tgcttcataa gaatatttat catgcatata atgaatgatg
     8881 ttcaacgtat tgacatatag ttctgctaac caatccatca tattcatgaa gttatgtttc
     8941 acttcttcaa agtctagtat ttctgatgtg attggacgat attctggtcc aacttgggcg
     9001 ccagtcactt catcgactcc gccattgatg gcatataata gtgttttagc caagttagca
     9061 cgagcgccaa agaattgcat gtcttttccg acaactgttc ctgatacaca gcaagcaatc
     9121 gcacaatcat cagaacccca ttgttcacga agctgattat cattttcgaa ttgaatcgaa
     9181 gaactttcga tagcgattct gctagcatat gttctgaatc cttctggaag gtgttcagaa
     9241 tacaaaacag tcaagtttgg ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga
     9301 cggaaatcat tctttgtaac taatgaacga ccatctaatc ccatacctgc aatggataaa
     9361 gtagcccaga tagggaatcc tgagaataat tggttatatt caggtgtacg cgcgaatttg
     9421 accatacgta atttcattac taagtggtcg atcaattctt gtgcttcttt ttctgtgatt
     9481 actccattat ccaaatcacg ttggatatag atatcaagga atgctgagat acgtccgatt
     9541 gacatcgcag caccattttg tgatttgatt gctgctaaat atccgaagta tagccattgg
     9601 acagcttctt ttgcatttgc agctggttta gaaatatcat agccatattt cgcagccatt
     9661 tctttcattt gcgctaatgc acgatattgt tcagtgattt cttcacgtaa acgaatcact
     9721 tcttcactca ttgtatgata accagtcata tcatgatctt ttttcttttg ttccatcaaa
     9781 tagtcgattc catacaatgc cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa
     9841 gcgtctggaa gccctgtaat gattttgttt ttacgagcag cacgcatttc agcagtataa
     9901 gcgtcaaata caccttgatt atgtgttttg cgccagtcag tgaaaatatg tgtcaattca
     9961 tcgtttggtg tatagccatt gctttttagc gcattgttag ccatattgat tccgccgaac
    10021 ggcatgaatg cttgtttcaa aggtttgtct gtctgtagac cgacgatttt ttccaaatct
    10081 ttatccaaat agcctgcatc gtgtgaagta atagttgata cgatttctgt gtccatatca
    10141 tagacaccgt tacgctcgaa ttggatatca tttaactttt gcaattcatc ccataatttt
    10201 tctgttgctt ctgttgggcc ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta
    10261 ttttgaataa agtctctagt gtctacgcct tctttccatt tggtaccttt aaatccattc
    10321 cattgctcca ttgtatggac ctcctaaatt tctgttttat gtaacagttt ttacgtgatt
    10381 agtataacac agcaccctat ctttgcaagg gttttcttgt tttttattta acaattttta
    10441 tgttttattt acatatattt aaatcaacgt tgacatacca acatttaagc attgtgaaca
    10501 ctttcatctt tttttgcaaa cgtataagga tttattaaaa atgaatgaaa aagagacgcc
    10561 acctgtatta atacagatgg cgtctcttca ttaaaatact acaacagtta cgactcgatg
    10621 aaaatctcag gagcatcgat aacttcgatg acttctccac cttttttctc atccatcgca
    10681 aatgatccat tcgatgttcg atcacctata gggaaatcag ttgggtcaat cgttaactgc
    10741 tgcccttttt gattcatcaa ggttatttct tttgatcttc cttccgacat atagataata
    10801 cggtgcggat tttttttcag ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt
    10861 tggctgattt cttgagcaag catgcgttta acagctcctc gctgagtaac gattacgatc
    10921 ggcgtatcac cttctgctaa cacaagcagc ccgttgataa tcctatctcc atctttcaaa
    10981 ttcatagact tcactccagc tgcctttgct ccaacaacgg gaacatctgt taaaggatag
    11041 cgcaaaccca ttccttgatg cgttactaag aaaacatcta agcctggctg atcttctttc
    11101 agatacacag caatcagttc atcttcttcc gcctttagct tcatacagct gagcggacgg
    11161 ctcttatagg tgcgccatgg ttcgaactct gtcattcgtg tttgcttgat caatccattc
    11221 ttgctgatga atacaaatgt tttctctgct tgaatttttt gatatgggaa aacagctaaa
    11281 atcgattcgt ctacagacag attggagatt gtttgagaaa tgtgctcacc agcatctttc
    11341 catttcaaat cgggtagttc atggacaggg cggtagatca catttgcttt atttgtgatc
    11401 agaagaacat ggtccaatgt actcaattca cctgagtata gcaagtagtc gccttctcgc
    11461 atcccgattt cttctggttt ggaagcacta taagaacgaa ggcttgttcg cttcacatag
    11521 ccttctcgcg tcacactgac aattacgtct tcttgtgcca ctaagacttg cgtatcaatt
    11581 ttgatttctt cgatttcgtc ttcgattgtc gttagacgag cggaagaata attttttttc
    11641 acttctcgca attctttttt cataacagag aaaagctctt tgtcatttga aaggatttta
    11701 ttcaactctg tgatttgcgc aatcaagctt tcggattcct tttgaagctc tgtgatatcc
    11761 gtgttcgtca aacggtataa ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat
    11821 tgaaagacgt cgactaaatt ctttttggcg tcttttttgt ctttactttc acgaatcgtt
    11881 gcgattactt catccagaat cgataaagct ttcatcaatc cttctacgat atgctgtctt
    11941 ttttgagctt tggctaaatc gaattgacta cgttttgtta tgacttgctt tcgatgctcg
    12001 atataacttc ttaaaatgtc tttcagtcca acttgatgcg gagtcatatg gtcgatagcg
    12061 accatattga aattgtaatt gatctgtagc tcagtgtttt tgaacaaata attcaaaatt
    12121 ccttgagcat tggcgtcttt tttcaattcg acgacaattt gcagacctgt tctatcactt
    12181 tcatctcgta cttcagctat tccatcgatt ttcttattca aacggatctc atccattttt
    12241 ttcaccaagg tcgctttatt tacttcatat gggatttctg tgatcacgat ttgctgttta
    12301 ttacccttca ttgcctctat ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt
    12361 tcataagctt ttttgatttc atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt
    12421 ccaggaatat attccatcaa tttttcaagg ctcgcttgtg gatgatcgat catatagatt
    12481 gtgccatcga tcacttcagc taagttatga gtcgggattt ctgttgcata tccagcagat
    12541 atacctgtcg atccattgac gagaaggttt gggtatcgag ctggaaggac tgttggttct
    12601 ttttctgtat catcaaaatt ccacacgaga tcgactgttt ctttctcgat atctgctagc
    12661 atctctccac ttaacttgga taatctggct tcagtataac gcatcgcagc aggtggatca
    12721 ccgtccatac tcccgttatt cccgtgcatt tcgatcagta cttcccgcaa cttccagtct
    12781 tggctcatgc ggaccatcgc ttcataaata ctgatgtcac catgaggatg ataattcccc
    12841 atgatatttc cgacagattt tgctgattta cggaatcctt tgtcatacgt attgccatct
    12901 ttgttcatgg caaataatat ccggcgttgc actggcttca atccatcacg gatatcaggc
    12961 agtgcacgtt cttggataat atatttggaa taacgtccga agcggtcacc catcacttct
    13021 tctaaagtta attcttgtac ttcttgtcga ttttccatca ggtgacctcc tattctactt
    13081 caaataactg attgttttca tttttatctg ctcgttcttc atctaataaa tcattagaaa
    13141 ctgaaggaga gatttcagta tcctcttttt tatctaagat actgccatct tcttcgagcg
    13201 taaattgcac gtgattttcg atccattttc ttcgaggttc tactttgtca cccatcagtg
    13261 tggttactcg acgttcagct tgcgcagcat catcgatacg gacgcggatc aacgtacggg
    13321 aagtcggatc catcgttgtt tcccacaatt gttccgcatt catctctcca agtcctttat
    13381 atcgctgcag catatatcct ttgccgactt ttttaatcat tgctgccaat tcgtcatctg
    13441 tccacgcata ttcaatgact tgcttctttc cttgtccttt ggatacttta tataaaggcg
    13501 gtaacgcgat atatactttc ccagcttcaa tcagcggttt catataacga taaaagaaag
    13561 taagcagtaa tacttgaata tgggcgccgt ctgtatccgc atcggtcatg atgattactt
    13621 tgtcgtaatt acagtcttcg atagaaaact caggaccgac tcctgcacca atcgtataga
    13681 tcatcgtatt gatttcttca tttttcaaga tatcttgcat tttagctttt tcggtattga
    13741 tgacttttcc tcgtaaaggt agaattgctt ggaactttct gtctcttcct tgtttagcag
    13801 atccgccagc agaatcccct tcgactaaat acaattcatt tttcttaggg tttcgggatt
    13861 gtgcgggtgt caattttcct gaaagaagag attcgccttt tttacgcttc ttcccatttc
    13921 ggctttcttc tctggcttta cgtgctgctt ctctcgcttc tctggctttg attgctttac
    13981 ggaccaacat ctggctcatt tcgctatttt cctgcaaata aaagcccatt tgttcactga
    14041 tgacgttatc tactacggta cgagcaactg gcgtaccaag tttttctttt gtttgccctt
    14101 caaattgcag aagattttca ggtacacgga tagaaagtac agcagctaac ccttctctga
    14161 aatcactacc ttccagattc ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt
    14221 cattataaga tttcgtcatc gccgctttca taccagcttc atgggttccc ccatcttttg
    14281 tccgaacgtt attgacaaaa gataaaacat tttctgaata accgtcattg tattgataag
    14341 cgacctctac ttcgatacct tctttttctc ctgaaaaata aacaactggt gtcaatgtat
    14401 ctttttcttc attcagataa tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt
    14461 ccttgactgg ttcttctcca cgaagatcag aaagagagat tttaacgcct tttagaagaa
    14521 aggcagattc tcttaaccgt tcggctaaaa tttcatatga aaatttcgtt gtactgaata
    14581 ttgtatcatc tggtaaaaaa tggacagtcg tcccgtttgc tttttttgtt tttcctatct
    14641 ttttcaaagt accgtcaggt ttaccgccgt ttttaaattt ctgctgatac tctacgccat
    14701 ctctaacgat cgttacaatt agccatttcg ataaggcatt taccacactg gctcctacac
    14761 cgtgcagtcc accagaagtt ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca
    14821 cagtaaaaat cacttcaact gttggtattc cggaagcatg cattcctaca ggcatcccac
    14881 gtccgctatc tgcaacagtt atactattgt cttcatggat ggtcacatca atctcactgc
    14941 cataacctga taatgcttcg tcaactgcat tatcgacgat ttcatagact aaatgatgca
    15001 agcctcggct gtcagtggat ccaatgtaca tccctggtct ttttctaact gcctctaatc
    15061 cttccaatac ttggatggac gagtcattgt attcgttgtt tacttttttt gccaaggcaa
    15121 acgctccttc aataatactt tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt
    15181 cgtgaaaaaa aagagggtag acgagaaatc aattttaatt atctcctaac cgctttttcg
    15241 acattcccct ctatcgatca tctcttcttt gtggtcattt ttatttttgg ttttctttca
    15301 acttaccaag ttcaatctta atacaacgat ccattacgat atctgtacgt cctgcttttc
    15361 ttagcatctc agctgcttct ttgttttcaa tccctaattg tgcccagaac actttagcat
    15421 cagttttcaa aaaatcacct gctatttcag gtaaaaattc actccgtcta aatacatcta
    15481 cgatatcgat tggatcatcg atttctgtca aattcgcata gactttctcg cctagaattt
    15541 cttcaccagc caataatgga tttactggta tgattttata tccattgtct tgcaatacct
    15601 gtgcaaccat gtaacttgtt cgttctggtt tattgctcaa gcctacgacg gcaatatttt
    15661 ttgcttcttc caaatagttg aaaatcgttt cttgtgttgg attttttaca gacatttctg
    15721 ccaccccttg cttttctttt tattccatct attatacttg tatctttcat tttttcaatt
    15781 gttcctgtta cctattcatg ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa
    15841 aatgaaattg atcttattac ttattttagc atatctttta ggttctattc cttccggtgt
    15901 ctggattggc aaattgtttt tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg
    15961 aacgaccaat acatttcgtg tattaggcaa aacagccggg attgttgtcc tgcttatgga
    16021 tattttaaaa ggtacattgg ctacttgttt gccgatgatc ttccaccttt ctgggattaa
    16081 ccctctttgg tttggggtat gcgccattct tggccataca tttccgattt ttgctgggtt
    16141 caaaggagga aaagctgttg cgacaagtgc aggaatgctt ttaggattta atcctgtatt
    16201 ttttgtttat tcttgtatca tctttatcgt tagtttattt tgtacaagta tggtgagttt
    16261 gagcagtatg atcagtgcag tattgatcac tttatctacg atcgtccttc cttatgtagc
    16321 tcctgtgatt ttggctaagc ccaattggtt actgacgatc attgcctttt tagtttcttc
    16381 ctttatcttt tatcggcata aagacaatat caagcgtatc agaaaccata ctgaaagtcg
    16441 aatcccgttt ggtttgaata gcagcaagaa aaaatcctga tactttttca ttccaaagag
    16501 ataaaacggg agtgtgacaa atcgatgtca cactccctta ttacttacag gatagctttt
    16561 ttacacacct tggtaaacgg tgttccctgc taacttctcg gtcacaacct cttacttcac
    16621 tgtgatggaa tattttgtct tgaagatttc atgggcacct agttgattga tccctacttt
    16681 ttccatcaag tttccactac tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca
    16741 aacgaaaggc gcttcttttg gataaggaga ccagatccct acataaggca tatctttata
    16801 gcttaaagtt acgctatggg aagatttttc acttcgaatg gcgtacgcat ttagacccct
    16861 tgtttcaaaa accaaggcat cttgctcaaa aagttcacga ttcaaagcca gattcgtatt
    16921 tgtctgacct aacgtctttt gatccatatc gatgaatggg cctttcaatg gtaggcgtaa
    16981 tcttgatttc aatggagaaa atgccagata gaaatcttca aatgacaatc cttcttcaag
    17041 aggtacattg aaagcaggat gtccaccgat agaaaagaac atttcttttt caccggtatt
    17101 tgccacttga taatgaaccg tgatgccgtc tcctcctaat tcatagctga tcactaattc
    17161 aaaatcaaat ggatactttt ctaatgtctc cggcgtactc cttaagatga aactagccat
    17221 ttcttgtcca tgttcaatga cttcaaaatc catatcccga gcaaatccgt gttgccccat
    17281 ttgatatgtt ttcccttgat aaacatactg atcatctttt aaacgtccaa caaaagggaa
    17341 taaaaccggt gcatgtctgc cccaatacgc tggatcacct tgccagatat actctaaacc
    17401 atgttctttt gatttcagac tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag
    17461 ctggtcattt tgaataataa cagtcattgc cattcctcca acttcgactt actttttaaa
    17521 atgggaaggc tggaacaaac atttctatct gccccagtcc ccttacttct aataactgtg
    17581 ttttgctaac tcgtctttaa aacgggaatt caatactttt aaataagttc ccttcatgcc
    17641 taacgaacga gattcaatga ttccagctga ttccaatttt cgtaaagcat tgacgatcac
    17701 agaacgagtg atgccgatac tatcggcaat attagaagca gtcagacgac cttcatctcc
    17761 atccaatgct tcaaagatag cttgtacagc cttcaattcg ctataagata atgtgttgat
    17821 tgccatttgg acagccgttg cactccgtac atctgcttcg atgcttcgag acttctgata
    17881 aaggatctgc ataccgacaa ccgtagctcc atattctgct aagactagat cgtcatctgc
    17941 aaacgattgt tccatacgag ataagataat cgtacccaaa cgttcgccag ctccaaagat
    18001 cggaacgaca gtcgtcagac caaatggata tttctctctt gactctaccg gaaatgcagt
    18061 aatatcacta gtaattggga tattagcttc agttttcatc aactgtgcca ctgtatcagt
    18121 ataagattca ggaaattgtt tctcaacaaa cattgttttg attcgttcat tgttcacatc
    18181 gtgacgttca ttataaccta gtaatactcc gtcacttcct atgatatagg tgttgctgtc
    18241 aagaatacca cctagtgtga cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa
    18301 agtattcttt tgctgcaaaa gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat
    18361 tgtcgtcctc cgatttttta taaaatatag cgacttaaat cttcatttgc cgcaattgag
    18421 ccaagtttgt catccacata tgcttctgtg atcgttattt ctcccatttg catatctggt
    18481 gcttcaaata atagatcttc tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg
    18541 atattatctg tgtcacggtt gacatcgaaa gcgatttgag cgatccgttc aattgcttct
    18601 tttgtaaacg taactgacac attttctgta ccgatcaacg cgacgtattg ttttactaaa
    18661 gcattatttg gttcggtcag aatcttcaca aaatcttcag ctgtcaaatc atctaattct
    18721 acccgtatcg ggaagcgtcc ttgaagttct ggaattagat cacttggttt agacaaatgg
    18781 aaagctcctg aagcaatgaa taggacgtga tctgtttgga tgatcccgta ttttgtgttt
    18841 acttgtgaac cttcgacaat tggcaatatg tctctttgga cgccttctct ggatacttct
    18901 ccagattgct gtgatttaga agtgatctta tcgatttcat cgataaaaat aattccactt
    18961 tcttccgcta atttgattgc ctcactatgg atatcagcat ccttgactaa ttttgctgat
    19021 tcttccttga tcaataactc taaagcttct ttgactgtta ccgtccgttc cacttttttc
    19081 ttcggtgtca aagcacctag agtttcattt aggtcgatcc ccatttgctc caatccattg
    19141 ttcatcattg gtgcaggttt cttttggtct tcaacttcaa tggttacttc acgactatcc
    19201 agtaaaccct tttgtagttg ttccaagact gtacggcgat tgattcgaat atcttctgtc
    19261 acttcttctt gcggttcagc ggtattttgc tgactagcat taaaaagatt catcatttgt
    19321 tcaaattgat tagtggattg tttctgttct tttttgatac caggtaccaa gatttttacc
    19381 aaacgttttt ctgcattttt tttagcactt gtgtatacac ggctgtactg ctctttttct
    19441 acgatttgga tcgcattttc tactaaatcc cgaaccattg attctacatc tcttccgaca
    19501 taaccgactt cagtaaattt tgttgcttcg actttgacaa atggtgcatt gacgattttt
    19561 gccaaacgtc gtgcgatttc tgttttaccg acccctgtag gtccgatcat cagcatattt
    19621 tttggtgtaa tatcttgttg cattttttca tctaactgca aacggcgata gcggttacgc
    19681 aaagcaacgg caacagattt ttttgctgcg tgctgtccta cgatatattc atctaattca
    19741 cttacgattt gtcttggtgt ttgttttaat tcatacatat ctactcacct cttacagttc
    19801 ttctacgata atattatgat tagtaaagat acagatatct gcggcaatat ttaacgcatt
    19861 ttctgcaatt tctttcgcac tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc
    19921 agccaacgca aaattccctc ctgatccgat agctaagata ccatcatctg gtgtgataac
    19981 ttctcctgta cctgaaacaa gaagcatttc tttatcattc attacgatca gcatcgcttc
    20041 aagtttttgc atagattgct gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg
    20101 tagattcccg ttatattcgt ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc
    20161 tgcaacactt ccggcgaaac ctacaacaac ttctccattg tagatacgtc gtactttctt
    20221 tgcagtgcct ttcatgacaa cagattcacc cattgttact tggccatctc cagccatagc
    20281 aaatttcccg tctttttcga cggcacagat agtggttgaa tgaaatggtg attcgaccat
    20341 gtttgtttcc tcctaaattt gttcttttcc ttatttttac gctcttggat ggaaagaacg
    20401 atagtttttt tgtaaacttt cttttgttac atgggcatag atctgggtag tcgataaatt
    20461 ggcatgtcct agcaattctt ggactgtccg caaatctgcc ccattattta ataaatgagt
    20521 agcaaatgta tggcgcagca tgtgaggatg gatatcacta tccaaactgc tttttttgat
    20581 catttgattc aagacatatt caattccagt aggcgtgatt ggttttccat gatgattcac
    20641 aaaaacgata tcatgctctt cgtgatattt tgtcatcaat acctggcggc cttccctgaa
    20701 atattcttgc agagcatctt gagcaaaaga acctagagga gcatatctct ctttgttccc
    20761 tttaccatgg atcaacatga cctctgaatc aaaatcaatg tctgataccg acaaactgct
    20821 acactcactt aaacggatgc ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa
    20881 atccaaagga ctatctcctg ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa
    20941 aaaacgtggc agtctcaaat ttttcttttt gagatgaacg taagaaaatg gattttcttt
    21001 tatcacttct tctttgagaa gatactgata aaacgaacgt aggctggcta tcttgcgact
    21061 gatagaattt ctgctatagc taggacgatg atcagctaat gcactcaaat agacacgaac
    21121 cgtaaaatat tccgccttca gcagatcagc gtctccagat tcctccaaaa aatgtttgaa
    21181 atggagaatg tcttcttcat atgcttcttt cgttttttct gaatatcctc tttcgacaat
    21241 caaataacgc aaaaattttt gttcccacgt ggcttctgtc attttctcac cttccttttg
    21301 tcccagagaa taatttagca cagcgtttat aaaaagacaa ataaattgtc agaatttaac
    21361 caatatttct aagtttgttc acaattggcg caattcctca cattttgttt gagaaattct
    21421 taagaaaaac gcaagaaaaa gagcgagact atccgtctca ccctcttcat ggagtttcct
    21481 tactcatttg ggattgcagt tttttctaat tctgttttta ttgcctcatt agcttttaaa
    21541 gctcgttgtg caattgcttc atatcttgct tttttgtctc gaatgcgctc tggcagttca
    21601 ggaaacagac catagttggc attcatcggt tggaaatgtt ttccttccgc atgagtaatg
    21661 tagtaagcca tacttccaat agctgtttct tgtggaaact cgactggttc catttctttt
    21721 gctaagcgtg cggcgttgat tccagcaagt agtccgctcg ctgcactttc aacatagcct
    21781 tctacaccag tcatctgacc tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc
    21841 ggtttcagca attctggcga attcataaag ctgtttcggt gcatcacacc atagcgaacg
    21901 aattcagcat tttctaaccc aggaatcatc cggaaaacac gtttttgctc gccccatttc
    21961 aagtgagtct gaaagcccac gatgttgtat aaagaagctg cagcattgtc ttgacgcagc
    22021 tggataacag cataaggacg tttgccagtt ttaggatctt ccaatccaac tggtttcata
    22081 ggtccgaaaa gcatcgtttt gaatcctcgc tgtgccatta cttcaattgg catacatcct
    22141 tcaaaatatt tttctttctc aaaagattta agttctgcca cttcggcatt gattagtgcc
    22201 tcatggaaag ctttgaattc ctcttctgtc atcggacagt tcaagtaagc tgcttctcct
    22261 ttgtcatagc gggacttcaa atatacttta tccatatcga tcgtagcttt gtctacgatt
    22321 ggtgcagctg catcatagaa ataaaaacca tctgaaccat tgaatgcttt gatgctttct
    22381 gctaatggat gagaagtcaa aggtcctgtt gctactacgg tgattccttc cggtatttca
    22441 gtaatttctt cgtttatcac tgttactaat ggatgctctt tgacttttcg ggtaattgtt
    22501 tcagaaaaag aatcgcggtc aacagctaag gcaccaccag ctggcactgc tgtttcatct
    22561 gccgaagtga ttactaccga attcaatcgg cgcatttctt ctttcaataa ccctactgca
    22621 ttagccaaac tgtttcctct taatgaattg gaacatacta gttcagcaaa attacttgtg
    22681 tgatgcgcct cagtcgattt gactggacgc atttcatata aacggacagg gactcctgct
    22741 tgtgcgatct gccaagcagc ttcacttccg gcaagccctg ctccaacaac atttaccatt
    22801 ttcaatgaaa gaacctcttt tctattattt ttggatgtct tcttcgtaat ctccatttac
    22861 acagactact tgtttaccgc ctttgacttt cttctcgacc agatatccgt cacattttgg
    22921 acaatttctg cccactggtt tatcccaaga agtgaattca cattctggga aacgggaaca
    22981 accatagaat agtctgtttt tcttagattt tcgttcaatg acttgtcctt catgacatac
    23041 tgggcaagtc acaccgattt cttttacgat tggtttcgta ttccgacaat caggaaaatt
    23101 actgcaagca taaaacttcc catatttccc taattttatc accatcggat gtccacagac
    23161 atcacaatcg aatcctgctg gttcatcttt gatttgaatt ttttcgattt tttcttccgc
    23221 attcgtcagt tctttttcaa acggtttata gaaacgatca accactttga cccacgcttc
    23281 cgtgccttct tcaattttat ccaaatctcc ttccatttct gctgtgaaat ggatatcgac
    23341 gatttgaggg aagaattcac agattaatga attgacaatt tctcctagtt cagtcggctc
    23401 aaatcttttt tgtgtcaatt ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc
    23461 ataagtggaa ggtcgcccta ccccgttttc ttctagtgca cgaatcaacg tagcctcact
    23521 aaaacgtgct ggtggttgag taaaatgttg ttttggttca atatcaacag attggactac
    23581 atcgccttct tcaagttctg gaagaatatt ttctttctct tctttgccgt catctctacc
    23641 ttccacatac acttgcatga atcctttgaa tttgattttt gaaccgttcg caatgaagat
    23701 gacaccattt tgttctaatg tcactttcat tgtatctaaa atagctggtg tcatctgact
    23761 agccacaaaa cgtgaccaga tcaatgtata aagtttcaat tgatctttat ctaaatattg
    23821 tttgagttca tctggtgtac gaagcacact cgttggacgc acagcttcat gggcatcttg
    23881 tgcgccttga gcatttttga tctttcggtg atcatgtcta gaaaattctt ttccatacgt
    23941 atcttcaata aatgctgcgg cctctgcttt tgctgtatcg gcaatccggg tagaatcagt
    24001 acgcatgtat gtaatcaagc cgactgttcc ttgtttaccc aaagtgattc cttcatataa
    24061 ttgctgtgct accatcatgg tttttcttgt tcgaaagttt aacttacggg cagcttcttg
    24121 ttgcaggcta cttgtagtga aaggattcgc agggtttctt ttgcgctctt tcttttctac
    24181 ttttttaact tcgaagtctg ttccatcaat tctagcagtg acttctttta cactttgtgc
    24241 atctggtaat ttctttttct taccgtcgat tccccagaaa tttgctttga atttcttgcg
    24301 agctttttta aaattaccgt cgatgctcca atattcttct ggaacaaatt ctcggatctc
    24361 tttttcacgg tcaataataa tttttaaagc gacagattgg acacgtcctg cacttaaccc
    24421 tttcttgacc ttacgccaga gaataggact gatcgaataa ccaacgattc tgtctaaggt
    24481 acgacgagct tgctgtgcat ctactaaatc tacatcgatc gttcttggtt ccttaaaagc
    24541 tgctttgact gcgtcttttg tgatttcatt gaagaccacc cgattttttt ctttcagatc
    24601 caacccaagt aggtacgcta aatgccaagc aatcgcttct ccttctctat ccgggtcact
    24661 tgccaagtaa actttttgag ctttttttgc tgcggctttc aggcttttga tgacatcgcc
    24721 ttttccgcgt atagaaatat aatggggttc ataattgttt tcaaaatcga tacccatttt
    24781 acttttcggt aaatctctta tatggccaac actggccatt actttatagt ttcgtccaag
    24841 atatttctca attgtcttgg cttttgcagg tgattctaca atcactaaat atttatacgc
    24901 cattactgaa tggactcctt tccattgtcg tgttcctact attaaaagag gattcataca
    24961 aatcgtagct aatcatatcc attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat
    25021 ggtgttattc tttaaattct gccaagatat cttcacccga aaccgtgcaa actgcaccgt
    25081 cttggatcaa atgatgacat ccggtagaat gttctgttaa aatatttcct ggaacagaaa
    25141 aaactgttct tccaagatcc aatgcttgct gtgctgttat cagagagccg ctttttttct
    25201 tggcttcaaa gacacatgtt cctttagaca aagctgcgat aatccgattt ctcattggaa
    25261 aacaatgctt tttcgggccg gattgaggag gatattcact caaaagaaga tgatcatttg
    25321 caatcacctc ttgcaagtca cgattttcag caggatagac ttgctctatc cctgttccaa
    25381 tgactgcgat tgtccgtcca ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc
    25441 ctttcgcgca gccactgacg atcacatatc cagcttctat cactggtttt agtaaataat
    25501 aaactgtttt tttggctaaa gcagaagtgt gtcttccacc aacaacgctt aacatgttac
    25561 aagaaagtaa agatagatca ccattataaa acagtacaat tggcggatag gtcaagtgaa
    25621 gcagctgagg gggatatttg ggatcaaagc aagtgataaa cgactgtttc gattggattt
    25681 tgccccagtt agcgtgaatt tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta
    25741 ttttacctat cttaatgatg tcttcaaatc cgatttcttt ataaggatac tgcatcatcc
    25801 agttagcaag tgcccatttt ccttgactac tgattccttt tgtataagca agtttcaata
    25861 aattttcttc tatttgatac atcataaaac cgcctttcgt ctctctttct aataagatac
    25921 gtaaaagcgg ctgtttttta ttgtaaatat ttttttacag gtgcaaaact tttacgatgg
    25981 attggcgtaa ttccttgtcg ttcaagtcct tctaagtgtt cctttgttcc ataacctgca
    26041 ttatgcgaga aaccatatcc aggataaagc acgtcatatt ctttcatcaa ttggtctcga
    26101 tatactttag caatgatact tgcagcagca atcgacacag atcgtgcatc ccctttgatt
    26161 attttttcct gctgaatagg caaatccaag gtcatcgcat cgatcaacaa atagcttggt
    26221 acaatggaca atttttctac tgcttggatc atcgctaatt tacttgcttc ataaatattc
    26281 acatcatcga ttgtctgctg atcaacgatc ccgatcccga ttgccagggc tttttcttga
    26341 acttgttgat aaagaagttc tcgctttttc tctgataatt gtttggagtc attcagccct
    26401 aatatgatct catttttcgg caagataact gcagctgcca cgacaggtcc agctaatggt
    26461 cctcttccta cttcatcaat gccggcaatc cattggtgac cttgtgcgta cccttgcttt
    26521 tcaaagcaaa acatttcttc atatttttcc ttaattaatg cttccttttt caatcgtcgt
    26581 tcaaaagaag ccagtgcttg ctgcacgcct ttgcgtggat catttcttaa tgattctagt
    26641 gtaggatcat ctattctctg tatgcttttc aatcgttctt tgatactttg gatggattca
    26701 gtcatcactt ttttgctcca tttcttcgca acgatccagt gtgtatcgtc caagttttcc
    26761 ttgacgaata tcatgcacga ctaattcgct gccacgatca tagtcttctt tatacccacg
    26821 ttttgctgta atatccatca gtaattccgg tgccaatttt tcttcttgct cgattgataa
    26881 accataacga tcgctgattc ttcctgaata atatcgggca aaaaagtcta atccgtaaat
    26941 cgctaaatca tctaaatgga caagctggtc tttgatcgcg cctgtcagcg ctagtttttt
    27001 gccgatttct tcatcttcaa atttgggcca taaaatccct ggtgtgtcta acaattccaa
    27061 ctcattacct aaacgaagcc actgctgacc ttttgttacg ccaggcttat ttcctgtctg
    27121 tgcaattttc ttgcctacta gtcgattcat caacgtagat ttcccaacat ttggaatacc
    27181 taagaccatt gcccgaatag cgcgaggctt gatcccccga gcagcatctc ttgcccgttt
    27241 ttctttcaat gcttctttag ccgcaggtac tagaccttta attcctttac tttcttgtgc
    27301 cgtgatagct aatgcaagat ggccttgttt acgaaaatat tcttgccaca gctttgtctg
    27361 ttgtggatct gccagatccg ctttattcaa taaaacaagg cgaggttttt gctgaatgat
    27421 ctgatctagc attggattac gagaagataa aggtaatcgt gcgtctacca attcaaatac
    27481 gatgtccaca tatttcattt tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg
    27541 aaaccattgg atggtcattg ttctctccat tcctttcata ccggaatatt ccccggcttt
    27601 acttgttttt ttctttttca atataaattc acttttttat tataaagaaa ttccatctat
    27661 tagttaagtg aaagtgaacc agtcctcatt ctatcacatc ttcacttttt tgatgatgca
    27721 aatttttatt ttggtttcta tatatgaatc taaatttttg acagcgtttc ctaaataaga
    27781 gatgatagac gaacaaaaca gaaaggagac gaaacaaatg aatagtatgg tttttgttaa
    27841 ttttcctgtt gctgatgtca atcgttccgt tgctttttat gaaaagctgg gttttaaaaa
    27901 aaatgaagaa ttttctactg aagaagctgg ttcaatggta tgggatgaca ctttttggat
    27961 aatgcttctc tctcatgatt tttataaaaa atttataaaa gataaagaaa tagctgataa
    28021 taaaaaaaca agtggtgctt tgatttcgtt tagtgttgaa aatgcggatg aagtaaaaaa
    28081 atttgctcaa attgcaaaag aaaatggtgg tactttccac gaagtagata tgggaatgcc
    28141 tgaagaagaa atgttttcat tagaagtcca agatctagac ggaaatacat tagaacccgt
    28201 atggatgaag atgtaagcag ataactaatc tgacgttttt tataacaaaa aaacaaaggc
    28261 ggatgatacg ataagtattc tccgcctcta ttttttttat tcgtacaatc tcttttttac
    28321 agtttataaa agcagtcttc tcaccctttt tttaattgtt agatgccagt gtatgtaaat
    28381 caccaatgtg tcatgacaca ttggtgattt tatggtatcc ttttattatt aactgacaaa
    28441 ggagagaata ttttgaacac tttgattgca aaagacgttt ttagattgcc cgaaatgaat
    28501 gcaagattgt tgtcgacctc tgctctttta ttagcccttg atgtcatctt atacaagata
    28561 gcgattggtc ctgcttattt tcaaatcaca ttcggatttt taagtatggc gcttatgggt
    28621 tatctatatg gaccgatctg ggctggtttg cttgaaatgt tgtcgaacac tttaaatttc
    28681 acgatatttg gttcaggaac gtttcaagct gcttttctta tcacggcttt ccttggtgga
    28741 tttattaatg gcttattttt atatcataaa aagataaacc tgccttccgt catcgctgcc
    28801 caattattga ttatgatccc agtcagcctt tttatcaatt cttggctgat gtctgttttg
    28861 tttggtgcag atttcaaagt aatttttcta ggtagattca tcaggaatat gatccaaatc
    28921 cctattcaaa taatggttgt ttattcattt atcaaaactt tagaggctag aaaagtgttt
    28981 aaacgagctg tttgatttta cgtactaagg tagtaaactg attttggatt aaacaataaa
    29041 gcgtgcagtc tcaacagact gcacgcttta ttgtttctca ctggatccat tttatttcat
    29101 caagtggata atagacaaat cttgctttac ctagaatatc agttttattg atcgtgccaa
    29161 acgaacgact atcttttgaa actcttcgat tatctccaag tacaaaataa ttatcttccg
    29221 gaagcttgga ttgtcccgtt agctcttgta gtgtaaagtc cgtcgtatag gatgcatttg
    29281 catgatctga tttcaggttt ttagttagat aaggttcctt aactactttc ccattgatct
    29341 tcaattgatc attttggtag ctgacatttt ctcctggaag accgattact cgtttgatat
    29401 aaatcgtgcc atctgctaat tgaaaaacaa caatatcaaa tcgtctgatc tccgaaaatt
    29461 tttccatcac aaccatatca ccttgcttca acacgttttc cattgaattg ccttccactg
    29521 gtaccgggat gaataaaaat ccccgcaaca tgaacgcaag gaatacagaa acaagtaagt
    29581 atttgaatac aagccagaag cgattaatat agcgttgttt ttttgtcaat ctctatcaat
    29641 ccatccctct ttttttcatt ctttctttat tataagaaaa atatcaaaaa aatgcgacta
    29701 gatagctgaa ataaaaataa ttctttcaat taggctattg gacaaaaaac ttttttgatc
    29761 ataaagtaca aaactatctc ttatatgtca tgattatttt ctaaatattt ttcccatgct
    29821 tcgtcaaaga tatccatgga cggcaaatag tcgacattca gttccagata attagaaatt
    29881 tcttcgtagc tagagctgtg cttaggaaat tggatgtctt taccggcttc tgtagcgaaa
    29941 acagataaag catcatgtgt gccaccgcct cgcagtgtca ttaaataatg aaaaaagctt
    30001 cgattcaccc ttcgttcctc caattttctt cgataaaggc agcccggcgt gcatggtttc
    30061 ttttaaaatt ttcaggatgc tttttataat actcttgatg gtcttcttct gctagataaa
    30121 atggttgggc tggttcgatt tttgtaacga ttggctgttc gaaacgtcca ctttcagcta
    30181 actgtttctt acttttttca gcaatgatcc gctgttcttc tgtcaaataa aagatcaccg
    30241 ggcgataatt gtcaccgcga tcctcgaatt gtccgaaagc gtcagttgga tcagtttgct
    30301 gccagtaaag ttctactaat tgttcatatg aaatcaatgt tggatcatag ataatctcca
    30361 ctgcttctgt atgtccagtc gtttggcttt ttacttgttc ataagtcgga tctggtacat
    30421 gccctccagt atatcctgac atcactgtat ggataccagg aagcgtgtcg aaaggctgga
    30481 tcatacacca gaagcagcct cctgcaaata ttgctcgttc cattatgatt ccccctctaa
    30541 tactactcat ccgatgattt tttattttct tttgtcttgc tttcagtact gctggtgcta
    30601 gttttgttgg tatttgagtt gtttgaagca tcagaggtat cttttggcaa atatagacta
    30661 aaccgaatgt catcatttac taagtcgatt ttatctgctt taatatacat cccgttttgc
    30721 attcggaatt gatccaacct aagcatgata gtcagatcat ctgtattgac ttcgatccaa
    30781 tctggtaatt tatagtttct cttgatcata ttcatcacat ctttgattgg caaactcaat
    30841 gttccgatag aaagacttct agcttttagt tggacatttc cgttttccat cacatacgga
    30901 tcaaaatata aatagaaatc gataggaaaa cctagtactt cgaaagtacc attgagtagt
    30961 gcttcatttt ccaaataaaa tttatattca atatcactat ctttttgata ctcactaagg
    31021 aaaaagctga tgatttgatt gactttttct ttatttgaat tgatcgttaa gacaggatct
    31081 ccttgtcttt ccgtaagagc tggtatttct gggagttctg gttcccgatt agcaaaaata
    31141 cggctgccta aaaatacggt cgaaccaagt actaccccaa gaaggataag gaaggcgaat
    31201 ttccagtaat ttgttttctt tgttttgttt gaagatacgt cagatcttgt tttcatgttt
    31261 attgatcatc ctccttctct aaccattggt tttgtgtctc gatcagctga tctctcactg
    31321 catttgccat caattgataa cctaaattat ttggatggaa atgatcttct tcatagagca
    31381 cattattttt gatgccattt agattttctg tcgtgctagt tgtttgatct gttccttctt
    31441 ctgtgacacc tacttcctga ttcaatcctt gataaagcaa atcattgatt gggataaagt
    31501 agcagttcgg catttttttt gtcaccgttt ctgttccttt gttccaattg ttaacaattg
    31561 tctgcatgtc agtgatctca ggaaagttca aataaaatgg attgtagatg cctaaaatgt
    31621 aaataggtgc ggttttattg agggatcgaa attcttccaa tagttctgtg acattttcct
    31681 gatagttttt cagtggcttt ttaaaggaat tgaccgttaa cccgaataaa ttattttgga
    31741 taactttcat caaatcatta ccgccaacag tcagtgtgat aaaatcagct gttttgatat
    31801 tgtttcgaat ttcttctttt gtttttactc gctttaagat ctggtcactt cgttcaccat
    31861 ttacaccata gttttctgtt tcaaccgcgg tcaaattata acggtctttt agatcatttg
    31921 cgacaagggg aacgaatcct ccttgcttgg tttgatctcc tactccttca gtcagcgaat
    31981 ctccgatagc tatgtagtga agtttttctt ttttatttgg atcttctctc acagtagtta
    32041 ctggtttcat tagtgggcca gctttcggta tagaagcagc taaacctgca tagatgatga
    32101 tcgcaattaa taatgggaac aaaacgagga tcaaatgtcg tttcgttaat ttcatttttt
    32161 cacctcttgt attcgattgg ttctaatgtt atcaaaaatt ttatcaaaaa ggtagtcttt
    32221 atgaaaaaag aaaacaatta ttttcctaat tttataaaga aaaagagaat aagctgcaag
    32281 cctttcaggc tgagggctta tcctcttttc gactaatcta cataatacat gattgcaaac
    32341 gcgtctttac cagtatgcgt agcgataacc ggattcgtat gcagtacagg gatatgcatg
    32401 tccgggaata attcttgcaa accctctttg aattcttgcg ccaattcttt tgcatccgca
    32461 tgtgaaatac cgatttgtcg cacatttggc aattctttca gttcattttt caaattatta
    32521 aaccatttat cgaacgtttt ctttcctcgc cccttagtta cagggattaa ttcagaatgg
    32581 gcgaaattca tcaccacgcg tatgttcaaa acactagaca gcaaaccagt tgttcggctg
    32641 attcttccac cttttaccaa gttatctagt gtggatattc cgatgtataa ttttgtattg
    32701 tcttgtactt ttttgacttc ttcaaggatt ttctctactg ttttaccttc ttttgctaat
    32761 tcagctgctc gaattacctg aaaagataat ccttggtcag tggtatcact gtcgataacc
    32821 gttactttcg tttttgttag attactggct tgtcttgctg cttctactgt tccactcaag
    32881 ccttttgtca tatggatcga aatcacttca ctgccgtctt ctcctagctc atcatacatc
    32941 gagatgaatt ctccaattgg tggttggctt gttttgggca gattttttgc tttcgccatc
    33001 atttccataa atctttcgcc gtctagttgg tcatcatcgg gatatactgt tccgtcgatc
    33061 atgatggata gcggcatgac acgaatgcct aaagcatcgc gtacattttt ttccatcgta
    33121 catgaagaat ctgttacgat ttttacattt gtcattattt tctctctctc tttctttaac
    33181 aagatgcctt attcatgtta aactacatgt aagacaattt ctatttttag tataacagac
    33241 aaataatgat ttttcattaa ttacacctag taaaggagcc gttttcatgg aaaaaacgaa
    33301 atttaatcga acgtatgaaa tattaaatga agtgtttaat gcagtcaccc atggaattgg
    33361 gactggattg agtatcgcgg gactagtggt tcttttagta aaaggtgccc acttgcattc
    33421 ctccctccat attgtctctt atgctatcta tggatcgatg atgattcttt tatttttaac
    33481 gtctactttg tttcattcat tgatttttac aaaagcaaaa aaggtgttcc aagtattcga
    33541 tcatagttcg atttttcttt tgattgcagg aagttatact cctttttgtt tgctgagtat
    33601 ccgaggatgg ctaggttgga ctttatttgt attaatctgg ttgtttgcga ttcttggcat
    33661 tatatataaa gcgttgacgt tgcataaacg ggagagagta tcaaaagtat ctacgatcat
    33721 ctatattttt atggggtggc tctgtgtcgt ggctgctgtt cctctttatc actctcttgg
    33781 aatgactggt gttattttga tggtagcagg cgggctttct tacacgatag gcgcattctt
    33841 ttatagcttg aattctatcc gctatatgca tgttgtttgg catttgtttg ttatgttagg
    33901 tgcaggtttt atgtacttct ctgttttatt ctttacttaa taaaaaaaat aaagctatgg
    33961 atgggacagc tctaaataac agctagtcta tccatagctt ttttatatcg tcaattcgcc
    34021 tgtcccgctt tttacaggat tctcattacg ctgatacgtt tcaaattgat gcggataacg
    34081 atttttttca tccatttctc cttgactagt attgatcagt gaccaatcag accagtcaac
    34141 aggtggaaaa taagcatctc catcgaatac atgatggatg actgtacggt ataatatcgt
    34201 acaaaatggc agaaattctt gataaacagc tgaccctcca gcgatgaaaa agataccgtc
    34261 tgtttctttt tccagagcaa gcacgtcttc cacactatgc atgacatgca cgccttctgc
    34321 tttataggaa gaatctctcg tcaaaacaat cgtctgacga ttcggcaacg gacggctacc
    34381 catcccttcg aatgtttttc gtcccatcac tagtgtattt gcttctgtca tttgtttgaa
    34441 gaatttcaga tcatttggca aacgccaagg aagacggtct tcttttccaa tcaatccgtt
    34501 ttcatcctgt gcccaaattg cagctaacat tttctcatcc tttctgattc taaacagcga
    34561 taggagcttt gatcgccgga tgtgggtcat atccttctaa ttggatatct tccatctcaa
    34621 aatcaaatac tgactgcttt tcctgattca aaactaaagt cggaaatgcg cgtatttctc
    34681 ggctcaattg ttctttcatt tgttcgatat gattgatata aagatgagca tctcctaatg
    34741 tgtgaacaaa ttcgccaacc gctaaacctg tttcatgtgc aattaggtga gtcagcaacg
    34801 cataacttgc aatgttgaac ggaacgccta gaaacacatc cccactgcgt tgatacaatt
    34861 ggcagcttag ccgtccctct tgaacataaa attgaaacat cgtatgacaa ggaggcaaag
    34921 ccatgctagg aacatcttcc ggattccatg cagaaacgat caatcttcta gaatcaggag
    34981 tctttttgat tgcttcgatc acttgtttga tttgatcgat aaagccaccg tcttttgttt
    35041 cccagtgacg ccattgatag ccgtagatgt gtccaagatc gccgtatgtt tcagcaaagg
    35101 aatcatcggt taggatcttc tcgcaaaaaa gcttgctctg ctttttatat tcttcattga
    35161 attcaggatc tgtcaaacat ctatggccaa aatcagtcat atctggtcca ttatagtctg
    35221 gacttttgat gtagcgttca aatgcccatt catcccaaat atggttttta ttttgtaata
    35281 gatagcggat attcgtatct ccttttaaaa accaaagcaa ttcgcttttg atcaatccaa
    35341 aaggtactcg tttcgttgtt aacaaaggaa accctttttg cagatcaaac cgcatttggt
    35401 atccgaataa gcttaatgtg cctgtgcctg ttctgtcttc tttacgatgc ccttcttcca
    35461 acaattttct tcctaagtca agatacgctt gttccatcct ttttcctcct aatcttctac
    35521 ccattcactc aagtattccc accgtgccat tttttcttca agttgtgctt ctgtttcaga
    35581 tacgtcgttt tgcaattctt gtaatcgagt gaaattatct ccttgatgat tcatctcttc
    35641 ttggagcaat gagatttttt cttcaagttc tgcaatctca tcttcaatcg tttcccattc
    35701 tttttgttcc atgtaagata atttcttctt ttcagcaggt tccttgctct gtatttttgg
    35761 tttcgctttt tcagcaggct gctgggtctc tttttgcttc agtaaataac tggacatgtt
    35821 tccgtaataa agttcttgtt ttccgtttcc ttggaatact aataacttat ctgctacctt
    35881 gtccaagaaa taacggtcat gggaaacagt gatcacagca cctggaaaac ttctaaaata
    35941 atcttccaaa atcgtcaatg ttgctatgtc caaatcattg gtcggttcat ccaataataa
    36001 aacattaggt tgctgaatca gcagtttcaa caaaaataac cggcgttttt ctccgcctga
    36061 aagtttgcct atcagtgtcc catgcataaa tcttggaaat aaaaatcgtt caagtaattc
    36121 tgctactccg atttgactgc catctgctgt ttttgcttct tcagcagctt cttgtaagta
    36181 agcaatcatc cgtttatctg gtgccatttc ttcattttct tgtgtataat acgcaagatg
    36241 aaccgtttca ccgatagaca ttgtaccgct atccagggga attctgccag ctagaatatt
    36301 aagcaatgtc gattttcccg caccattttt accagtaata cctagtcttt cccgagattg
    36361 aatcaataaa tctaaatgtt ctagcagtgt ttggtcattt atacggtaac tgccatcttt
    36421 gatttccagt acttttttcc ctaaacgttg tgtcgctagg ttcagctcta aatcatcttc
    36481 ttggtttact tggtacaagt tctcctttag atcttcgaaa cggttgatcc gtgcttgctg
    36541 cttggtgcca cgtgcctgta cgccagccct catccaagct aattcctgtt tataaagccg
    36601 cttgcgcttt tcttcttgtt ctacttctac acgatcacgt tctgcttttt ctaagacgta
    36661 agcttcataa tttcctttat actcgtataa ttttccaaat gaaagttcga agattcggtt
    36721 tgctactcta tctagaaaat aacgatcgtg ggtaaccatc aatagtgcgc cacggtattg
    36781 tttcaaatag ttttccaacc actcgatcgc ttcataatct aaatggtttg ttggttcgtc
    36841 tagtaatagt aaatcgggtt catcaatcaa cacttgcgcc agactaactc gttttttctg
    36901 accacctgat aaagtactga tttttttatc taattcactg attcctaatt tttgcaaaat
    36961 aatctttgcg ttggtatctg tagtccaagc atcttcttta ttcatttgtt cttctgcttt
    37021 agcataacgt ttttgcacat cagggtcttc tccgttttcc cctagttcga tcaatgcttc
    37081 ctcatatgct cgaacagttt ggatcaaggg gctgtttcct tgaaagactg cttggagaac
    37141 agttaattca tcggaaaatt cagctgcttg agaaagataa ccaatacggt aatcagttgg
    37201 ataaaacact gtttgccgat caccgtcgct gctatcgata ccggcaataa tatttaagag
    37261 acttgtttta cccgtcccgt tcgtcccaat caacccgata cggtcttttt cgtggaccaa
    37321 aaaagaaatc tggtcaaaca aatctttttc accataagtt tttttgaggt cagttacttt
    37381 taattctttc aacatgttcc ctccttatct tggatattgt atcataaaaa tagtgaactt
    37441 caggaaaatt tttttggtat ttgttctttt tgtgttctca aaaaaagaac aggaccgatt
    37501 cgatcctgtt ctgatatgtt ttgtttttta taaaacttga aaatttttac ctttttattt
    37561 tattggactc gttttttttg actttgctcg aaacgcaaat cggaaagaaa agttatcaga
    37621 tggtttgttc atcgtataag attctaacaa attgatattc ctgcgatagc tgttcgcaga
    37681 aaacgtcgtg attttttcag caacttcgta ttcatcaatg atttttcttt caagataata
    37741 acctcttagc atttttttcg taaacagact atcttgctgg atcatccacg aagaaatcaa
    37801 attccccgag ttcattttta ttccttcaag tttgcgttgc tggataaaaa attgaaccat
    37861 cgtttcatca taaatatcag tcaagttatc taaactttca ataatcaact gagtattttt
    37921 ttgatatgtc tctctgattt cttggatatc ttcatcacta ataaatgtct gacgatcagc
    37981 atatcttctt tgccaaaaca ttttcggatg gagaacgatt cgcattaacc tacggacaac
    38041 aatgaaccaa aagccgataa aagacaaaat ctggcttgtg atagaatgtt cgaatcgaga
    38101 aagaaaacgg gagtaaaaac gataaccatt agaagaaagc ttgcccgaac gataactttc
    38161 atccaatcca tcccgttcga tcgacaaaat cagtgcttgt atttcttgta cttcttgctt
    38221 ttcatcatcg gttaaatctt ccaagtacag atcacgtatc cgttcttgat aattttctat
    38281 cactgcttca gtggctaaaa attcttttgt attacgatca atttccttta tttcttttcg
    38341 caaaacttca acgacctctt ctaaaatcat caaagcattc aaatccgtgc ttttcgcttc
    38401 ctctgtttcc gtcagcatag gcaagacaat gatccctatg accaaagtca ctaagatgac
    38461 acccgccgtc agaaacaaaa gcaaagaccg ctggtaaaac atcatattat tgattgaaaa
    38521 tggcaaaata aaaatcgtag ccaaactgac tgttcccttt acaccgccaa atgttaagat
    38581 caagatctcg ttcaactgtt ttttgaactt tttactgcca tctttgaata cataaaaaag
    38641 actgatagaa atgaatctga tcacaaaaag catgacagag attaatacga taatcgctaa
    38701 taaaagaccg tttggatata aaccgtctcc ccatactggc gagaacactt gcgtcaattc
    38761 gattcctaaa aatataaata ctaatgcatt cagtgtaaaa gcaatagttg tccatgtact
    38821 gtgggacaag ctcgacaatt ctgcatcaaa cacagagatt ttcctaaaac cagaagcttg
    38881 gagaatacct gctgccactg cagcaatgat tccagataca tcaaatgcct ctgccaatac
    38941 ataggctaga aaaggaagca aaagttcgat cagcagatac cctgtgacat cttgtgctga
    39001 tgctttttcg atcaaatgaa ttattttttg tttgaaccag acaagcaaaa agccgatcaa
    39061 agctccccca atactagaaa ctactagact catccccgcg tttactgctg aaaaagatcc
    39121 ggtgatcagc gcaccaagag caaactggaa ggcagtaaca ccagatgcat cattcaaaag
    39181 accttctcct tccaaaatat gcatcgcctt tttagggata ttcactcgtc cagaaagaga
    39241 actgactgct accgcatctg tcggacctaa cgctgctcca aaagcaaagc aggcagcaag
    39301 tgggacactt ggcaacagga atgataacgt cgcacctacc ccgacaaggg tcaaaatgac
    39361 tcctccaaat gccaagaaca aaattgtccc gaaatttttg agaatcgaag aaatatctgc
    39421 tttttctcct tctctaaata acaatggtgc aataatcata actaagaaca attcaggttc
    39481 aaaatcgatt gattgtcccc actctgtcag acctaatacc actccaagga aaatttggat
    39541 cagtggagct ggaatcattg gcaaaatgcg gctagctatg ttcgataagg tgatcgttac
    39601 tgcaaataca ataactaact cagcaaattc catcgtccat cccctttatt ttttattttg
    39661 tgtaagcatt tttaaaaatt gttctttttt ctttaaaatt ttggtttcga ctacttcaac
    39721 ctgtgtttgg ttcccctcta attcacactg tacacgtttt gactccaaac gaagcagatc
    39781 atgtttcaga cgttccaact gacgatacat caatatattt cttttacgca acttacaaag
    39841 tatttgacgg cggtcatcca tcgaaagggc gccatagcga tcatgcaaca gttttttttg
    39901 ttgttcaaaa ctctctctca ttttccagat ctcctataca caaacttcca tcattatagc
    39961 aaagttcttt ttggatgaaa aatatttcat tgtttcatcg tttattgcta gttagaaatt
    40021 tccataaatt tttcaacgcc tcattctatt ccaaatagta tcagatatca agtaaaactt
    40081 aagaaagata aaagtttctg acaaaaggat gtttactttc ccattcctgc ctttcttgtt
    40141 cttttatcac aacaaaaaaa tggggctgcg acaaaaatct tgtcacagtc ccttattctg
    40201 aataaacggt gtggaagaga cagcctatca gtagtaataa gacacatctc tttcacagcc
    40261 tcatttttgc gtaaaaatcg atttattcga aaaattcccc gtatacatct tctacacgtt
    40321 gatctggatt gatcacgata tacaagttac ctgttttttc gctgattttt gttgattgat
    40381 attgattgtc taatccttct gttcctttat ctagatatgg gaagtacaca agatcaggtg
    40441 tgccatttcg gtaaaggatt tccattcgct cgatatcttc gtatttcaat gcgcgattaa
    40501 acatgcccat ttccagtccg cctaggttga tatcatgtgt cgcaactgcg tccccttcat
    40561 ggaagatttc gattttaaat ccagcacacg gatggatttc aacaaattca ctgccattga
    40621 ttcgtccaaa acttgttgtg atttgtttga tccaaagatc gccgatatga cggcggttga
    40681 tcgtccaggt ttctccattt ctaaaataaa attttagcgc tgtcatttct ttactcatta
    40741 aaaagactcc tttatgatgt aatttattct tgtactacct cagtttagca caaatttatg
    40801 taagtggtca catcttatgt tttttcttct aatgacgtat ctgcccactt taagtagcca
    40861 acaaagctga gcagatacag tctagtgttt tttaaatcaa tttttctttc gataaaaaat
    40921 ccaaaacctg ttttttatca ttttttattc tttgttctac gacggctttt tcagctaatt
    40981 gcaaagcttc actaacccac ttaccaggtt tttttccagt ttcttctagc aagattttac
    41041 catctactgc taattcgctt aatgacttga taggtaaacg atcataggat tctcctacgg
    41101 catccatctg aggtgtttcg tcaaaataga tcatcaattc ttctacttgc agtgcatgtt
    41161 ttttgcctaa gaagtacaat tcgaatggtt ccaaagctcc agtttctcgt tgtcgtagtc
    41221 cttggacaag tgcttgaacg tctttaatca tttggttaga acacttccac gatttcaaaa
    41281 aggaacgaat ctcattttct tttagatcca gttggctgat caataatgcc catgcttggc
    41341 tctcttcttt caatgggacg gatggtaatt cagcaaaacg taaaagtgct tcgccttctt
    41401 ctttcaaacc gggacagcaa atgtagcact ctgtttcaat aaatgcatgg attcccgcgg
    41461 cccgttcatt tcccaagagc atcttgatga attccacatt tatacgttct acagatattt
    41521 tacctaatag ctcgtggttt tctttgatgg cgtagaaggt ttcttcctct aatttgaacc
    41581 ctagttgact gacaaaacgc aacccgcgca tcattcgtaa ggcatcttca tggaaccgct
    41641 catgaggatt gcctacagct cgcaagatct gttcttctag atcttgcaga ccatcgaaca
    41701 aatcgatgat ttctccatct tctttcaaag caaacgcatt gatcgtaaaa tctcttcttt
    41761 tcaaatcttc tgccaacgaa cgaacgaatt cgacatgatc tggtctacgg tagtcctgat
    41821 aagttgattc cgtccgaaat gtggtgatct catattgttc atcattttct aataccaaca
    41881 cagttccatg ttcgatccca atatctatcg tttttggaaa aagctgtttg atttcttctg
    41941 gaaatgcgct tgttgcgata tccacatcat ggattggctg gttcagcaag atatcacgaa
    42001 cacttccacc tacaaaataa gcttcatatc ctgcattctc taattttttc agaactggaa
    42061 ttgccttttg gtattccaga ggtaattgtt ttaatttcat ttttccaacc tttcctgcgt
    42121 gtttcttgtc ctgagataaa gactcagttg catggtgact gtttgacaag tccgtttcag
    42181 agtcttcaag gccaggacca cttcatcttg tttactcgtc cttacgctca aagcgattat
    42241 ggtctctttg attgaatttt tccatgtttt tttcaaaaac ttcagttaag tcgatattca
    42301 atgaattagc catgatcatc gtaacgaata aaacatcacc aagttcttct gctaccgagt
    42361 taactggttc agaagccttt ttgctttttt ctccataagt gtggttgact tctcttgcca
    42421 attcacctac ttcttcagtt aatcgagcca tctgagctaa tggagagaaa taccccacct
    42481 tgaattgctg gatatattca tccacttcct gctgcatact gtataaagaa cgctttttat
    42541 ccatcttttt ccctccaaaa ctatcttacc aaaactttaa cgcaaatcct agataagagc
    42601 aattgagaaa atcgaatttc catgctacat ttaggaagga atttctaaat ggaaattctg
    42661 tgaaaataca taattgaaag ctggaattca tatggtcaaa cgaaaaacag taaaagaagt
    42721 tctttttatc attataggaa caagtattta tgcatttggt cttgtttact tgaacatcgc
    42781 taatcattta gcagaagggg gagtctcagg gatcaccttg atcttaagag ctttatttgg
    42841 tattgatcca gcttatacga ctttattaat caacatccca ttgattttac taggaggaaa
    42901 gatcctcggt aaacgttcct tggcctatac tgttctaggt acactttctt tgtcattctt
    42961 tttatggatc tggcaaaggg ttccactaga aatcaattta cagcatgatc tactgatcgt
    43021 ttctctactt gcgggtttag ttgcaggtgt aggaagcggg atcgtctacc gcatgggtgg
    43081 aacaacaggc ggcagtgata ttatcgctcg gattttagaa caaaattacg gcatcactat
    43141 ggggcgttcc ctattggcat ttgatatcat cgtcttactt gcttcattaa cctatatcga
    43201 cttgaaacgt atgatgtata cgttgattgt ttcgtatgtt ttcagtcgtg tgattgattc
    43261 gatcttagat ggcggatatt ctgcaaaagg tctacttgtc gtctccaaca aaagcgaaga
    43321 gattgcacca ctactgatga ccggtctgca aagaggtgtg actttccttt ctggagaagg
    43381 cgcattctct ggtaattcaa aaaaaatcat ctatatggtc gttagctcta gtgaattaac
    43441 tgaagctaag aggatcgtcc acgagattga cgaaaaagct ttcctttcaa tcatcaatgt
    43501 gcatgaagtg gaagggcaag gctttactta tttgaagcca cagacaagat tactgaaaaa
    43561 actataagaa aaaagacaaa gataggctat gaggaaagat tccacatcca gctgaccttt
    43621 gtctttttta gtactatact atttttaact aattgttatg ttcacttacc cggttacttt
    43681 tcttttgtgt ctgcaaaagg atctttcggt taacttcatc agataatttc aaaaaagcat
    43741 ccctatctcc atcttctagt gcttgatcga tttgagcata aagcattttt agttttgcct
    43801 cttcttcttt ttcttgaaaa tatcgttcaa ttaattcagt atcttcttca ctgactgaag
    43861 aatcccaaga agaaaatgga ttgtcttcta agatagatag atattctctt gtctgccaag
    43921 cattttcgaa tatacattcc acatataaag gctttttcca attcaaacgg atttcatgaa
    43981 aaatctggtc tgtgtccgta aattcttttc cctgcaaaaa caatttcatg ggttcccctt
    44041 caacagatag atcactgatc tgcagtcctc gatccgcaaa attcgcccct tcaacaaagt
    44101 ggacattgct taaaatcgct tcatgattgg caagataatt caagatccaa aaaacttctc
    44161 ttcggctaaa agatacatga ttgaccatcc acgttaaaaa attcttcttt tcccgtacat
    44221 caattagcat ttttctcacc tctctgttag gtcatctaat aatgacagtg cctccatatc
    44281 tcctggttca agtgttaaat aataagtcaa taattcttgc gctcttttca gctgcccttc
    44341 ttctcttaag aaaaaggcat actctttcaa gaaatctggt tcatgtgata gttcatgata
    44401 agcctgttca tagtgtacag ctgcaacggt aaagtcttct aattcattat aagcatgggc
    44461 taggttccat tctgcataag gattagcagt ttcttccatc tgattgatca cttgtatcac
    44521 atcttcatat ctttcatcat ccaagtaaag attacttaaa gtaagtaatg tttcatcttg
    44581 tttgtctcct acttctaaag ctttcagtaa aagttcttct gctcgctgtc tatcatgcag
    44641 acggaaagca ttttccgagg ccaggtgata aagttcgact tggtaaggat tttctttgat
    44701 tccttcttct aacactgttt gtgcttcttc aatcatttct tcttcttgaa gagcttgtcc
    44761 tagatataga tataacgatt gataatgcgg attaacttct cgaagttctt ccagatagtt
    44821 gattgcctgc tgattttctt tcaattgaag attcgtaaaa gctagctgga acagtcgatc
    44881 atctgtcctt tcttctttca gtgcttcttc taaaggtacg atagctgctt cgaattctcc
    44941 ttgcatactt aaggattgtc ctaaacgttc ctgcatcgag attcctgaaa tttcatttat
    45001 cccgcttgcc aacaatgttt cataaaccac tgctgcctct ttgaaacgat caactgaaaa
    45061 atacaattca gctagcgcaa actggatcaa tggttcatca ggaagcaacc cagctgcttc
    45121 tttcaatttt gcttcactca cttcagggat tccgataact tggtacaaat cagctatcgc
    45181 caataagctt tgcggatagt aatcactttg cttatccact ttttccaggt attcaaaagc
    45241 taagtcgatc tcattatctt cgattgcaat ttctgctaac gggatgttca gtccgtcatt
    45301 ttctgggtat tggttgatca gttgttcaaa aatctgtttt gcttcgatca aaaagccaat
    45361 agccagcaat tcttcgccta gttcagctaa tatatctgct gggtcttctt ttaatgcttt
    45421 ttctaaaaag atttgtgctt gtgacaagtc ttcatctgat aaagcttgca acattttttc
    45481 gctatttgta ttcatttcaa ttccctctta tctcactgac gatttgttga aataccgtca
    45541 ttgttctcaa taattctttt tcgtttggat gttggtatcc gtctattata cctatttttt
    45601 ctaaaacaag tatttttttg cttttttctg ccaaaaaaag aactttttcc aaatactcca
    45661 tctttgaaat ctgttccagc cagtggattg gatagccaag caagtccagc cacacaaaaa
    45721 agttttttag ttggaaatca aaaggatatg cttggagatt ccacacgaaa tgaaacatca
    45781 ttcctaaaaa ttttttcata ctagcagaaa gtagatggcc attttcggtc agataaaaag
    45841 cttgttcaaa cagtttacca taatttgaca ggttttctgc ctctaattga taaaaataga
    45901 tcatttcttc gatcatcccg gcaaacggcc gagtcgttaa ttgcttttta tttggatagt
    45961 ttttatacag attttgcaaa aaacgatgat cacaaatcat tccgcaacag ataaaagtca
    46021 aaaagtcaat ctgctttccg tccacctgcc gctcgctgat tgtttgatca taaacgatgc
    46081 gttctggcag attgatggtt tgcagcacta cacgatttgg tagctgcaaa atcgtccgct
    46141 gcggtgttaa tgctcggctt agagaacgga tcgatacagg gatcatccat agtgatgaat
    46201 caagggttgt atgtttttga aaaaaacctg ccagatccat gatcccttca tttccaaatc
    46261 cgatgataaa catggattga ttttcgggat agcgtttgac gaaatcaagc aggttcttta
    46321 attctgttaa atgattgcat tgagtgttgg cacaaatata ccagtcattg tttgcttgat
    46381 ttgcaaataa cgggttgatc ttatctgcaa acaaatcata atagcgctga ttcgttaaaa
    46441 ataagattgg ctgatcaatg gaaagttctc ttagctgact gactagcgtt tctccgtaaa
    46501 acactttcgt caacgcatct tctacttgat aatcaagttc catacgcatc cctcctcttc
    46561 ttatttttat tttaccatta gtaagcaaat tctagaaaag ataagagcaa agctcacctc
    46621 ataacttgca aataaataaa aattaaataa aatacatgtg ttttctatgt atgacatacg
    46681 gattcggaat ctctgtctct tatttattcc ttttctgcta ggaaataaaa agaaaagcac
    46741 ttgtagccct agagccacaa gcgctttttc aactgtctta tttaacagcg tcttttaatg
    46801 ctttacctgg tttgaatgca ggtactttgc tagctggaat ttcgatttct tcaccagttt
    46861 gtgggttacg acctttgcga gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta
    46921 attgaacttt ttcgccgtta gctaaagcat cttgaatagt tgaaaataca gcatctactg
    46981 ctgcagttgc atctttttta gttaaatcag tagctgaagc aactttttcg attaattctg
    47041 ctttgtttgc catggataga ttcacctcct ccacaagaga tatgatagaa atatcattta
    47101 gttaaaactt tctgagtggt ccagaagttt aaaaagaaat attgattaac ttcaatattc
    47161 tgttatcacg atatcatatg aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct
    47221 ttttttagtc atagaaacgt aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc
    47281 gatatggtaa aatgcctatt ttctcctacg agggataatt cggatggacg tcccttcaaa
    47341 agtaaatgct tttctgattt gattttctaa gaaacgttca taagaaaaat gcatcaattc
    47401 ctcttcattg acaaaaacga caaatgtagg tggtttgaca gcgacttgtg tagcataaaa
    47461 aatcttcagg cgttttcctt tatctgttgg tgttggattg attgctacag catccataat
    47521 gatatcattc aacacagccg aagagatacg aagattctgg ttcatactta cttgttcgat
    47581 catttcaggc agacgttcca accgttgttt agtcactgca gaaacaaaaa cgattggagc
    47641 gtaatcaaga taccggaatt catcacgaat ctcttgttcg aaatcacgca ttgtattggt
    47701 ttctttcttt acaagatccc atttattcac gacgataata attccacgac cagcttcatg
    47761 ggcgtaaccc gctactcgct tgtcttgttc ccgaatccct tcttcagcat tcagtaccat
    47821 caaaacgata tcagaacggt caatggcacg cattgcacgc atcacgctgt atttttcagt
    47881 attttcgtat acttttccgc gtttacgcat accagctgta tctatcatta agaatttctg
    47941 accactttca gaaacgaagt gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga
    48001 aacaattaca cgttcttctc ctaaaatcgc attgatcaaa gaggatttac caacatttgg
    48061 gcgaccaatc aaactaaact taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa
    48121 atacttcact gcttcatcta aaatatcacc aatccctagt ccatggcttc ctgaaacagg
    48181 ataaggatcg cctagcccta aggaataaaa ttcatagata tcgtttctca tttctggatt
    48241 atctacttta ttgactgcta aaatcactgg tttattactt ctgtataaaa ttttcgcaac
    48301 catctcatct gcgtctgtca cgccttcgcg tccgctgact acacaaatga ttacatctgc
    48361 ttcttcaatc gcgatttctg cttgatgctt gatctgatcc ataaaaggtt catcgccaag
    48421 atcaattccg cctgtgtcga tcacgctgaa ttctcgacct aaccattcac cttttgcata
    48481 aatacgatca cgagttactc caggagtatc ttcgacaatc gagatacgct caccagcgat
    48541 gcggttaaaa atcgtagact taccgacgtt cgggcggcca acgattgcaa ttgttggatt
    48601 tgccatatta tttcctcctc acaagtttcc aatctcactt agtttaacga atgcattgcc
    48661 aactttcaag catagattgt caattaaacg aagtattttt attccttatt gaaatactaa
    48721 tcgcttttca aaagaaaaac tgagacaaaa gcagctgctt tcatctcagt cacagataca
    48781 aaatcaatta tttttcagaa tcatcggttg ctttttcttc tacttcttct ttcaacgcat
    48841 cacctaaaat gtctcccata gtaaatccag tattttcttc tggtaattca taagactcaa
    48901 cattttttgg ttcttcttct gaagctggtt tttcttctaa agctttgata ctcaatgcaa
    48961 tacgatgttc ttctggatgg atctctaata ctttcacttt cacttcgtca ccttcatgaa
    49021 gcacttcatg tggtgtcgca atatgtttat gtgaaatttg agagatatgg actagccctt
    49081 ctactccagg gaacacttca acaaaggcac cgaagcttgt caaacgttta actgttccgt
    49141 ctaatactgt gccaactgga gcttttgtat cgatttctga ccaaggtcca ggctgtgtgt
    49201 ctttgattga caaggaaaca cgttcttgtt caggattgat agacaacact ttgacattta
    49261 catcatcatt gacagccaat acatcacttg gttttgctac atgactatgt gaaatctctg
    49321 agacatggac tagaccgtca attccgccta ggtcgacaaa tgcgccaaaa tcagtaagac
    49381 gtgcaacacg tccactgaga acatctccct catggatagt tgaaagaatt tcttttttct
    49441 tttcttctct ttcgttttcc actaccgctt tatgcgataa gatcaaacga ttttctgatg
    49501 gttcgatttc gatgatttta aagctcaatg ttttgccttt atagtctgaa aagtctgcaa
    49561 caaaatgatc ttccaccatt gatgcaggga caaaaccacg tacaccgacg tcaacgacta
    49621 aaccgccttt gacaacattt gtaacaggtg cttcgatgac cttgccctct tggaaatctc
    49681 tttcgatatc ttcccaaact tttttagcat ctaaacggcg ttttgaaagt agatagctgc
    49741 cgttttcttt atctttgccg atcgtactga taacgactaa atctaataca tcaccgactt
    49801 ttactacttc gctgatatct tctaccggca aagttgataa ttcttttgcc ggaacgactc
    49861 cttcaactcc agccccttcg attccgacaa tcacttgttt gtcttcaatg actaaaactt
    49921 cgcccttaac aacatcgcca acttttactt cttgtacgct tttcattgcg tcttccattg
    49981 actggttgtt ctcttgattt tgatcaaatt ctgtcataac acttgtcctc ctaaccaaca
    50041 ttctgccgta agtacggtac tgtttttagt ttaattgatt attcaaaaaa aataaagcga
    50101 tttcgttcgt tttccttgaa aaaacaattg tttttcattt tttttcttaa tttaaaaaag
    50161 aagataacct tttttcaaaa caacagcttt tattgctgca acgacttctg gaatcgattt
    50221 gccagtcgta tcgatacgaa cagcatcctc tgcttgcttc agcggagaga cgtcccttgt
    50281 tgaatcataa tggtctcttt cctgtatctc tttcgtcagt gtttcaagat cagtcggaat
    50341 tcctttttct tggttctctt tgaaacgtct ttctgcacgc tcacttacgc ttgctactaa
    50401 aaagattttc acttctgcat tcggtaatac agctgtcccg atgtctcttc cgtccatgac
    50461 aacgccaccg cgttccccta tttttcgttg gacagcaact aattcttctc tgacttttcc
    50521 atgtgcagag acttcagaaa cagcttttgt gacatctggt tgacggattt ctaaagtcac
    50581 atcttttgat gctatgaata catgctgtcc atcttcttgt tgcgaaaaag agataggata
    50641 ctttttgatc agcgaaacta gcccttcttc atctgaaaaa gatacattgt tcgtaatagc
    50701 taaataagtt actgcgcgat acatggcacc ggtatctgta taaatataat tcaattcttt
    50761 tgctaatatc ttggcaacgg tacttttccc agatgaagca ggcccgtcga ttgcaatact
    50821 gatttgtgtc atgatataac tcctttttct cagaaaaaaa gccgcaaagc gcggctatta
    50881 ttatttaatt cttaattctt gaccagggta aagcataaag ttgtttggat ctaagccatt
    50941 caattcaaac aattgatcag tggtgatccc tgcacgctca gccacttgtt ttggaccttc
    51001 gcctgcttga acagtcgttg ttgaaccggc tgcagctgat gaagaaggtg ttgtttctgc
    51061 tgcattgctg cttgaatccc ctgcttgata agttgcctca cttgaggaag tcagtgttgt
    51121 ctctgctgca atactgcttt ctgacgttac acctgtatct tgagacacag tagattcttc
    51181 cgcgattgta ctagattcta ctgttgaagt ctcagttgac tcctcttttg ttgtacttgt
    51241 agaactttct actactgatg tcgaagtttc agaagaagtt gctgctgttg cattgttgct
    51301 tgatccattt ctgatccaga aataagcacc tgctggaata gcgatacata agaccagtaa
    51361 aatcaaaaat agcgaaagaa ataatgtatt tcctttcttt tgttgtctct gagaacttct
    51421 tgatgaaact tcatcatctg tatcgtagat tggttgttcc catggttctt gtgtttcgtt
    51481 attttctgta gattgatgtc tatcttttct gctcactgtt ttacctccta aactctatcg
    51541 tgtgtattat accataggaa aaagtgtctt gtcctaaaaa atcaatgttc catgtttttt
    51601 ttaaataaat ttagtaaaat cgtttcccaa tgaagttgtt cagtcttaga aaaaagcttc
    51661 ctctttggca gaagttctgc ttttgctcca tcaatatcac aacaattctg cggcttttcg
    51721 gagagttttt cgccaaaata agctaagatg aattttctgc ggcagttttc ttcattaata
    51781 tagcgaagca tctgctgcag gcggaattct ttttcttttt cctgacgttt gagccgttcc
    51841 acccaatttt ctggtttttc agactgctgg atcagctcca tccatttttt ttgcagctca
    51901 tcaaagggag cttgctctgt ggcatattcc aaataaagct caaaactttg tcgctgttct
    51961 ctagaaagtt gattaaagaa ataatggata cgttcatccc cactttgata aaaaagaatt
    52021 gctgcacttt cttcctgatc tctgcctgct cgaccgattt cttgtacata gttttctaga
    52081 ctgtctggca aatcataatg gaccacataa cggatatcag atttatcgat ccccatacca
    52141 aatgcattcg tagcaatgag aaactgcagc tgatttttta caaactgctg ttgaagttgt
    52201 cttctttgag ctgcatcaag accaccgtga tagtatccaa ctgtaaaacg ttccctgaat
    52261 aaatgataca atcgttcgac ttcttttttt gttgcacaat aaatgattgc cgctccttgt
    52321 gctctttcca tgaagtgttc aagttcttgt tctttttgct gcgttttccg aacaaaaagc
    52381 gagatatttt gccgattgac tgattgtctg atctcttttg gtggtcgatc aaatagtaca
    52441 tgacgtatat ctcttgctac taaatctgtt gctgtcgccg ttagtgccag tgtgaccggg
    52501 tttcctaaaa gctttcggat ttttcctaac tgttgatatt ctggacggaa atcaactccc
    52561 cattgagaca cgcaatgagc ctcatctact acatataacg caatattttg ttttttcaga
    52621 tgctctaaaa gagaaggatt agtcaacatt tcgggactca aaaacaaaaa tttatattgc
    52681 gatagatgac ggaggacgta tcttctttca gattgcgaaa gtaaactatt gaaagcagct
    52741 actcgtttct cccctcgttt ttgtaattgc gtcacttgat cttccattag agaaagcagc
    52801 ggtgacacga tgatgaccag accttccatt aaataccccg tcaattgata gcaaaggctt
    52861 tttcccgttc ctgtaggcaa aatagcgaga gtatcttctc catcaagcaa cgcctgaata
    52921 atttcttctt ggccgggacg aaatgaatca aatccaaaat atttttttaa ttcatttatt
    52981 aacattctgt atcgcttcct ttctcaatag atagatttga tacaaacgaa attctccata
    53041 atctaattgt ccatctacat tccattcgct aaagcgccac tctcgcacgg aatccttatt
    53101 tggttccaaa cgttcgattg tttctgcaga gagaatccac tcgtatggaa aatcatcaaa
    53161 aaacaaggcc cactcaataa gatgatctgt tactgttcct ctttttaaat gacggattgc
    53221 catgatctgg tcaattgtct gaccattcat aaacatctgc ttggtgatca tcatgctttg
    53281 attgaaattt tgctgtaata atgaatcgat caaagcgtgc caaagcgaat ctggccgttt
    53341 ttcgatttgt gctaacaaaa ggtggataca tctgctttgt tgcagataaa ccgctgactc
    53401 atcattgttt tttgccagct gataaggaag cagccctgtt cgctcatttc cagataattg
    53461 gttagctaaa aaatctgctg cttcttctgg tatctccgaa aacagctgca ccaaactctc
    53521 atacgcagta tcaatcaaaa tttcacgtgg caattggctt tcactaagcc atttttttag
    53581 ttggaaagta taaaaaggac ttgtctctgc tggaagatag tcttgaatac ccgttgccag
    53641 attagaaatt acttgaacag caaatttaat gagccgccaa ctagtttccc ctgttctgcc
    53701 atagcggtca aaacgcaaac cagtatgttc tatttgatgt tcggacaaca tatcagcccc
    53761 tgcaggcgtt agctttgctt cattttcatt tatttcgatc caaccttgct gcgcaatttt
    53821 ttgcaaaatt tgataaaact tatcctgctt caattctgga aaactgttat ggataaacaa
    53881 aagttcgtgg gtaaatccat aaataagtac tgaagtcgtt cgttttccca caaggagttg
    53941 gtaaagagaa ctaatccgca gcttgtgtcc tgatgaaaat agcgataaaa taaaaacaat
    54001 catattattc tccttaagtt aggatggtta gaaatgtcac tactatgtaa acttgtacct
    54061 gaacgctgta ttgcatgcgg tctttgtcaa atcgaagcgc cagatatttt cgattatgat
    54121 gaagaaggaa tcgttttatt tgcccaagaa ccagaggctc atcaacagtt cgtgatgccg
    54181 gaagaagaaa catatgtaaa aaaagcgtat caaaaatgtc cagttcgagc gattttatta
    54241 gaaaataaaa tataaatata aacgaaactt taaaaacgta cactaagttc tctaaacttg
    54301 aaaataagcc gggatctgtt tctcttgttc cgtgaaatcc cgacttattt tgaaagattc
    54361 atttgctatc tatagttaat tgactagttt tttcacggac cctataggac ccatttatat
    54421 tatttcaact ttttcagcca gttttccaat gtattgacga tcacagtttg ctggtctgcg
    54481 ttagaaattt ctgcctctcc atctcctttt tgctcaccat agctgccaaa acctccatga
    54541 tttccgccat tgattgatac atactctgtt ctttccggta aatactgctt agcttcttga
    54601 taagcttcct gattcaatac tttatctttt gtcgccgtga tcgataaaac aggaacagct
    54661 gattctgtca atgttccttt tttgtcggga taacttgcca tgaagaatac cccttttaaa
    54721 tgtttgtctg aatgattctt attgacataa cgacttgcca ttacgccacc taatgaatgg
    54781 ccaccgatca cataatcgga atttggaaaa tcttctttga ccttatccgc tttgtttccg
    54841 cccatcactg ctagatcaag tggcattttg acaatagcga ctgaaaagcc gttttttgct
    54901 aaatcttgcg cccaaatact ataactttct ggctctacga gagcaccagg ataaaaaata
    54961 agtaatggag actttccatg attctttttc tctccttcaa aaaataagta atcttctttg
    55021 ttttttcctt cttttaccaa cacttgcgct tctgacgatg gttcgtacgt attttgctgt
    55081 atatagtaat aaccggctcc accgagtaat cctaaaatga tgactaagcc aattagtatt
    55141 cgtctaaata ttttcacttt ctcccacctt ttctatctga gtgacccctt tttcttaatc
    55201 aagtatgatt ataccttaaa aaaataagag agaaatgcaa actttccagc atcaaataaa
    55261 aaaagacgga atatccattt gaatatccgc cctttttgta tgtttatttt tatttagcac
    55321 ataaacgata cacagcttct gcataaattt ccattgcttt atacatacta cttaattccc
    55381 atcgttcatt cgcctggtgc ataaagtcag gagtatcagg gaacatcgca ccgaatgcca
    55441 cacactggtt catcgtacga gcaaaagtag cgccgcctga tacaaacggt tcagtcatgt
    55501 ctccagtaat atcgcgatat gtccccaata gatttttcac taactcactg tctagtggca
    55561 cgtataatga atcaaggaag tcaaattctt cataagtcaa cccatatttt tctgctgttt
    55621 tagttaattt agctactaaa tcatctttct tgaatgtaac agggattctc atatctacac
    55681 cgatttttgt ttgttctggt gtgatttcca aacttgcaaa gttcatcgtt agctcgccgg
    55741 attgttcatc ttctgttttg cccacgacag cttcaccagt tgcattttct tgaactaatt
    55801 ttcctaagaa attgattggg ccaaaatcaa atacttctga aagagcaatt gccagacgag
    55861 aaatagcatt cactccttgt gctgcatctt tcgcgtggat acttttacca agaacgacga
    55921 tgccttcacc ttgttcttcg aaatcgaatt cgtgtttttt caatgcttct tttacttcag
    55981 aaagcttttc tccagagtaa ggagctgcat caggaacaac attcaacgct cctccagctt
    56041 ttacagaaaa ctcgttcgtt cctggtcctg tcaagtaagc ttgcaataag cctttttctg
    56101 cataaattaa aggaaattca gcatctggcg caaatccttg cgtaattcct tcttcttttt
    56161 cgttatattt ttctaaacaa cgccataaat tttcttcatc tgtaccaaag ataaaacgaa
    56221 tacgtgtctt gaattccacg ccagcatcaa tcaaagcttt gacggcatac attgccgcaa
    56281 tagaaggacc tttgtcatct tgggatccgc gaccaactaa ccagccgtct tttatagttg
    56341 gatcgaatgg ttttgtttcc caattatttt cgtcccctgc tggaacaacg tccatatgac
    56401 acaagatacc gaataattca tctccagagc caatctctga ataaccgtaa tagccttctg
    56461 gatctttaaa tgttctaaat cctagctttt cactgatttc caatacttta tccaaagctt
    56521 caatcacttt tttaccgaag ggatgtcctt gtcctgaatc tgcttcatct agtacggaag
    56581 gaattcggat caattcgctc aatgattcta atgcttgttc atgatgttgt tctgtcacaa
    56641 attttttcat gatattatcc tcctaatatg ttaaaaaggg tcatgtcaaa ttcctccgac
    56701 acaacctctt cttctcaaat aatgacgttt taagcctgct ccatcagcaa gcagaatgtt
    56761 cttaacaaat gatgaaagaa aaaacactca ctgaatggag ctattgactt atccacgtgc
    56821 ttatcgatag ccgtttatcg gtttcttcat tcttatgata gaccttgtac ctgtatttgt
    56881 ctaggtttag aacattgctg ctacacctag gatcacacag atggctacaa ataatatcgc
    56941 aattaattta ccggtaaatt tccaccacac tgaaacatcg aaacgagcaa ttgctacagc
    57001 acccattacg atagcagatg ttggtgtaat taagtttact aaaccagatg cagattgata
    57061 agcagtgatt actaaatcct taccgacgcc ggcaaattca cccataggtc ccatgattcc
    57121 cattgttgca gcagctaagc cggaagttga aggaatcaag aatgacatag ggatatagaa
    57181 aataaatgta ataatgatga atacacttga agataatgaa cttaatcctt gttcgcccca
    57241 atgtaagatg gtgtctgtga ttaatccatt gttcataacc acttggatac cacgagcaac
    57301 agctactacg attgctacac cgatcaaatc agaagcacca gctaaaaatt cagagacaaa
    57361 ttcagactct ttcatgccat aagtgattgc tactacgatg gacattacta agaataacat
    57421 agtaatttct gggaagtacc agtcacctaa tggaagcatg cttttaccta gaaccgtacc
    57481 tagtaccgga aggtttgtta accagtttgt caatgattca aaaatcgtga aattcgcatt
    57541 caaggttgtc caaggaatca aactgatgat catgatacca aaagtaataa agaacaatac
    57601 attaacatgt ttttgttttt tcgtgattac ttcttgacga ccgattgctt caatatcgaa
    57661 atgcttcata tcttctttac gttggttgta tactaatgat tttgttggat ctttttcgat
    57721 ctttgatgca taacgataca catatacaat agaaatagct aacaaaataa cgaacatcaa
    57781 gaatcgaaga ccaatacctt cacccatcga aatcccaaca gcttgtgaag ctacgcctgt
    57841 tgcaaaagga tttaccgttg aagctagaca gcctacttga gaccctacta atacgattgc
    57901 tacagctact actgtatcaa agcctacagc catcatgact gggatcagta aagcatagaa
    57961 tggaatcgtt tcttctgcca ttccgtaagt agagccgcct aatgcaaata acagcatcaa
    58021 tactggaata agcatttttt cttttccttt gaatttcttc acaactgaag caatcccggc
    58081 atctagtgcc cctgttttgt tgataatacc taaaaatcca ccaacaacca aaatgaagaa
    58141 agagattggg attgcagcag gtgtttcttt agttagctcc cccgctggta cacctagcat
    58201 cccattgatt ggcgccatga aaatatccca cagcccttga ggattttgtt gtactcgttc
    58261 atatgatcca gcgacgatgt tgcctgcatc gtctacacta tagtgcccag ctggtataaa
    58321 ccaagtaagt acagcgatta ctgcaataat aataaataag accgtatacg ctgaaggcat
    58381 ttgaaatttt ttcttttttg tttgttccat cttcttcttc ctttcttttc cgttagagac
    58441 aacaattttg gctctaatgt tattttatca aaaagagtat cagaacaaaa ataataacac
    58501 gcattttgta aaacttttgt tcaaaaagtt cgtttttccc tattccctcc tcttttttca
    58561 ggtttttaaa gctttcttct ttttcattgt agcgtttcct tttttttata aacaaacatt
    58621 ttcgtctttt aaaaggagaa gaatttcaca tttgcaaaga attgattgat ttttatgcat
    58681 aaatcaaaaa aatactcaaa acaaacaaat aaaacgttta cattattgca tgtcttgtat
    58741 ttttttccaa aaaaaaagac caagaaattt
//
LOCUS       DACEOM010000008        55255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEOM010000008 DACEOM010000000
VERSION     DACEOM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.008"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(135..2288)
                     /locus_tag="IW781_000473"
     CDS             complement(135..2288)
                     /locus_tag="IW781_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1181493.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2582..3430)
                     /locus_tag="IW781_000474"
     CDS             complement(2582..3430)
                     /locus_tag="IW781_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1181494.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDLK"
     gene            complement(3654..5594)
                     /gene="thrS"
                     /locus_tag="IW781_000475"
     CDS             complement(3654..5594)
                     /gene="thrS"
                     /locus_tag="IW781_000475"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1181495.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5931..6110)
                     /locus_tag="IW781_000476"
     CDS             complement(5931..6110)
                     /locus_tag="IW781_000476"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1181496.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6157..7980)
                     /gene="cadA"
                     /locus_tag="IW781_000477"
     CDS             complement(6157..7980)
                     /gene="cadA"
                     /locus_tag="IW781_000477"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1181497.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8258..10537
                     /locus_tag="IW781_000478"
     CDS             8258..10537
                     /locus_tag="IW781_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1181498.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10630..11277)
                     /locus_tag="IW781_000479"
     CDS             complement(10630..11277)
                     /locus_tag="IW781_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181499.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11456..12175)
                     /locus_tag="IW781_000480"
     CDS             complement(11456..12175)
                     /locus_tag="IW781_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1181500.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12306..13457)
                     /locus_tag="IW781_000481"
     CDS             complement(12306..13457)
                     /locus_tag="IW781_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1181501.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13674..14414)
                     /locus_tag="IW781_000482"
     CDS             complement(13674..14414)
                     /locus_tag="IW781_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1181502.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14418..14741)
                     /gene="rsfS"
                     /locus_tag="IW781_000483"
     CDS             complement(14418..14741)
                     /gene="rsfS"
                     /locus_tag="IW781_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1181503.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14798..15391)
                     /locus_tag="IW781_000484"
     CDS             complement(14798..15391)
                     /locus_tag="IW781_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1181504.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15375..16022)
                     /locus_tag="IW781_000485"
     CDS             complement(15375..16022)
                     /locus_tag="IW781_000485"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1181505.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16035..16346)
                     /gene="yhbY"
                     /locus_tag="IW781_000486"
     CDS             complement(16035..16346)
                     /gene="yhbY"
                     /locus_tag="IW781_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1181506.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16362..17471)
                     /gene="yqeH"
                     /locus_tag="IW781_000487"
     CDS             complement(16362..17471)
                     /gene="yqeH"
                     /locus_tag="IW781_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1181507.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17468..17995)
                     /locus_tag="IW781_000488"
     CDS             complement(17468..17995)
                     /locus_tag="IW781_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1181508.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18175..18960)
                     /locus_tag="IW781_000489"
     CDS             complement(18175..18960)
                     /locus_tag="IW781_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1181509.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18953..19822)
                     /locus_tag="IW781_000490"
     CDS             complement(18953..19822)
                     /locus_tag="IW781_000490"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1181510.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19823..21202)
                     /locus_tag="IW781_000491"
     CDS             complement(19823..21202)
                     /locus_tag="IW781_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1181511.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21210..21629)
                     /gene="fabZ"
                     /locus_tag="IW781_000492"
     CDS             complement(21210..21629)
                     /gene="fabZ"
                     /locus_tag="IW781_000492"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1181512.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21654..22130)
                     /gene="accB"
                     /locus_tag="IW781_000493"
     CDS             complement(21654..22130)
                     /gene="accB"
                     /locus_tag="IW781_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1181513.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22133..23368)
                     /gene="fabF"
                     /locus_tag="IW781_000494"
     CDS             complement(22133..23368)
                     /gene="fabF"
                     /locus_tag="IW781_000494"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1181514.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23387..24124)
                     /gene="fabG"
                     /locus_tag="IW781_000495"
     CDS             complement(23387..24124)
                     /gene="fabG"
                     /locus_tag="IW781_000495"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1181515.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24136..25053)
                     /gene="fabD"
                     /locus_tag="IW781_000496"
     CDS             complement(24136..25053)
                     /gene="fabD"
                     /locus_tag="IW781_000496"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1181516.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25160..25384)
                     /locus_tag="IW781_000497"
     CDS             complement(25160..25384)
                     /locus_tag="IW781_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1181517.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25437..26399)
                     /locus_tag="IW781_000498"
     CDS             complement(25437..26399)
                     /locus_tag="IW781_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1181518.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26409..26849)
                     /locus_tag="IW781_000499"
     CDS             complement(26409..26849)
                     /locus_tag="IW781_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1181519.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27155..28120
                     /locus_tag="IW781_000500"
     CDS             27155..28120
                     /locus_tag="IW781_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1181520.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28177..28407)
                     /locus_tag="IW781_000501"
     CDS             complement(28177..28407)
                     /locus_tag="IW781_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1181521.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28493..29380)
                     /locus_tag="IW781_000502"
     CDS             complement(28493..29380)
                     /locus_tag="IW781_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1181522.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29469..30692)
                     /locus_tag="IW781_000503"
     CDS             complement(29469..30692)
                     /locus_tag="IW781_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1181523.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30709..31788)
                     /locus_tag="IW781_000504"
     CDS             complement(30709..31788)
                     /locus_tag="IW781_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1181524.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31919..33646)
                     /gene="ptsP"
                     /locus_tag="IW781_000505"
     CDS             complement(31919..33646)
                     /gene="ptsP"
                     /locus_tag="IW781_000505"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1181525.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33646..33912)
                     /locus_tag="IW781_000506"
     CDS             complement(33646..33912)
                     /locus_tag="IW781_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1181526.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34391..36625
                     /locus_tag="IW781_000507"
     CDS             34391..36625
                     /locus_tag="IW781_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1181527.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36773..37093
                     /locus_tag="IW781_000508"
     CDS             36773..37093
                     /locus_tag="IW781_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1181528.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37298..37522)
                     /locus_tag="IW781_000509"
     CDS             complement(37298..37522)
                     /locus_tag="IW781_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1181529.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37720..39300)
                     /locus_tag="IW781_000510"
     CDS             complement(37720..39300)
                     /locus_tag="IW781_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1181530.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39701..41077)
                     /locus_tag="IW781_000511"
     CDS             complement(39701..41077)
                     /locus_tag="IW781_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1181531.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41205..42323)
                     /locus_tag="IW781_000512"
     CDS             complement(41205..42323)
                     /locus_tag="IW781_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1181532.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42465..42899
                     /locus_tag="IW781_000513"
     CDS             42465..42899
                     /locus_tag="IW781_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1181533.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(42977..43510)
                     /locus_tag="IW781_000514"
     CDS             complement(42977..43510)
                     /locus_tag="IW781_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1181534.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGGSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43604..44782)
                     /gene="mutY"
                     /locus_tag="IW781_000515"
     CDS             complement(43604..44782)
                     /gene="mutY"
                     /locus_tag="IW781_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1181535.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44775..45572)
                     /gene="recX"
                     /locus_tag="IW781_000516"
     CDS             complement(44775..45572)
                     /gene="recX"
                     /locus_tag="IW781_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1181536.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45679..47061
                     /gene="rlmD"
                     /locus_tag="IW781_000517"
     CDS             45679..47061
                     /gene="rlmD"
                     /locus_tag="IW781_000517"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1181537.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47235..47813)
                     /locus_tag="IW781_000518"
     CDS             complement(47235..47813)
                     /locus_tag="IW781_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1181538.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48135..48605
                     /locus_tag="IW781_000519"
     CDS             48135..48605
                     /locus_tag="IW781_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1181539.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(48713..49939)
                     /locus_tag="IW781_000520"
     CDS             complement(48713..49939)
                     /locus_tag="IW781_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1181540.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(50693..50893)
                     /locus_tag="IW781_000521"
     CDS             complement(50693..50893)
                     /locus_tag="IW781_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1181541.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(51027..51635)
                     /locus_tag="IW781_000522"
     CDS             complement(51027..51635)
                     /locus_tag="IW781_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1181542.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(51720..52190)
                     /locus_tag="IW781_000523"
     CDS             complement(51720..52190)
                     /locus_tag="IW781_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1181543.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(52238..53152)
                     /locus_tag="IW781_000524"
     CDS             complement(52238..53152)
                     /locus_tag="IW781_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1181544.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            53298..54101
                     /locus_tag="IW781_000525"
     CDS             53298..54101
                     /locus_tag="IW781_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1181545.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            54279..55226
                     /locus_tag="IW781_000526"
     CDS             54279..55226
                     /locus_tag="IW781_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1181546.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT        15892 a        11666 c         9441 g        18256 t
ORIGIN      
        1 ttttttgccg agtaaacggt gttcaaaagc cagtttctcg gtatgaagtc aaatttaagc
       61 aaaaatgtga gaagctattt tcacaaaatt tttcttcata ctcgaaacta agcggctttt
      121 tcacgacctc ttttttattt tgccatgaac atatcataat acgcgtccat tatttcttta
      181 gcaatcgtca agttcatgtg gtcttgttcg tctaataagt tcggcaagac aacactgatg
      241 gcaatttccg gattgtctgt tggtccgtat gccacgatat tactattgac tgtcgtaata
      301 tctggatgac cttccgcaac cgtttctgca gtccctgttt ttgcggaaag gtccattttc
      361 gcactggcta atggtttggc tgctgtgtaa ggatcggtac catggacaac ttggtggaat
      421 ccttcacgga ggatcgccat attttctgca gaaatattga ccgtattttc tactgttgtg
      481 ccaatttctt tcttcacttc acccaagttt ccattttcgt cattgccata gatcgctttg
      541 actaggtgcg gctgtactcg tttcccgcca ttagcgactg ttgaagcata ttgagctaat
      601 tggattggtg tatatgtgtc aaactgtcca aatgccaaat cggtaaagtt acctggagta
      661 taccattctt tgccattatc cgcattgaac ttcttcatat aatcaacaga acgtgagatc
      721 ccaggagatt cattcggcaa atcgatccca gtcgttgtac caagaccaaa ctctttgaaa
      781 gagtctctca attgctgata agctttagct tcttcatcta aactaggtag tcccattcca
      841 ggtgtatagt ctagtcctaa catctttaac gccactttga tcatatacgt attagaagac
      901 aattccaatg ctttgactgc atcaagagca acttgtccgc ttcgattaaa gacagagctt
      961 ttttctgaag caccttgcag acgaatcggc tcatcaatca aaacttgatt tccgctgatc
     1021 gcgttacttg cccagcctgc tgtcaaagta ccagccttga ctacagaccc tggagcaaag
     1081 gcgctagtaa tcgtgcctaa agcattctcg gtgatttctt tggaaccttg ttcatgactg
     1141 aaaccagtca ttgctagcac ttctcctgtc tgaggattca tagccactgc ataagcacca
     1201 ggcgaatact gtgctttgcc gttgttgatc aatgtttgat aattcctttt caaaatttct
     1261 tctacttttg attggaattc tgcattcata gataacatta aattagaacc tttttcccca
     1321 gagaagattt ctttttgatt cgaaacattt ccttcattat caagggaaac ttcatattgg
     1381 gttttggttc cttgcaggac atcttcatat tgtttttcca aataactttg tcctacacgg
     1441 tcattccgag aataaccttt tgctaggtat ttctctgctt catctgctgg aagtccttgc
     1501 ttttcagtcg tcacactgcc aagaatactt ttcagtgaac ttgctgcatt atattctcgt
     1561 gtccagtctg tgccagtcga aactcctgga agttcagaag cgcgttctgc cacaacagct
     1621 aactcttgat cagtaacacc agagtttttg atatagacag tgtttaattc atatgcacta
     1681 ttcatccgtt taaagatggt agcaattttt aattgctcct cgtttaaatt aatctcatca
     1741 gctgtgactt tattgaccat cgcactatat tgttcgctgt tccctaattg cttttctttt
     1801 gccgaaagtc tttttgtggc ttctttcaaa tgtttgggat cagccagcca aaaatctttt
     1861 ttatctctat ctgtcagatt ttcatctacc ggaacatcga tcagcgagtt cagcttcgtt
     1921 gcgatttcaa gcaaatcttt agccgtaact tgtgttcctc tcgtatatgt gatggcctga
     1981 ttcgctttat tcgtaactaa agcttttcct gtggagtcgt agatcattcc gcgaggagta
     2041 ctttcttgga ccgtaatcgt agaagtagac ttcactcgcg cctccatctc ttcgccgttc
     2101 acgatctgca aataacccaa tctagcaatc aacgcaacaa ataacgtaaa tataatgaaa
     2161 aaaagaaaat ttaagcgaaa aggaacatgg gaccggcgaa acgttttgtt cttaggttct
     2221 tgtgcacctt ttcttatttt ttctaaccaa gaagtattta atattttttt cataaaatct
     2281 ttcatcattt cctatccttc cgcctattta cattcatgct tcattgtacc caaaaacttc
     2341 ttaataaaaa agaaaaaatg ggcggagcaa gaaattcttt acctaatttc aagataaaat
     2401 caactttttg tttcacagca atcacttaaa gttacatatc caactatttt tttcattaaa
     2461 aaataaactt atttttgaaa attatgtttt cttaagtcgt gaaacacttt gatggatcat
     2521 ttatttgtgg aaaactacgt tttgttattc taaaaaactt atataaaaaa tagtaaggtt
     2581 tttatttcaa atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc
     2641 taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg
     2701 ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta
     2761 cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt
     2821 cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt
     2881 ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt
     2941 ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt
     3001 ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac
     3061 actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta
     3121 cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat
     3181 cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg
     3241 tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg
     3301 aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt
     3361 agttggccaa ctttttttct gctgaagaat aatcttccat catactttca atacgactaa
     3421 ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga
     3481 cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat
     3541 cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta
     3601 tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt
     3661 tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct
     3721 acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg
     3781 tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc
     3841 attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg
     3901 atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata
     3961 ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt
     4021 tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct
     4081 tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat
     4141 aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag
     4201 gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc
     4261 attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga
     4321 tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc
     4381 atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga
     4441 ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca
     4501 tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc
     4561 gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg
     4621 ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat
     4681 gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta acctagacta
     4741 acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc
     4801 cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct
     4861 agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct
     4921 ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca
     4981 cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga
     5041 acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct
     5101 tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat
     5161 gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc
     5221 atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta
     5281 tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg
     5341 cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct
     5401 tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca
     5461 acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt
     5521 tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa
     5581 gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct
     5641 gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt
     5701 tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt
     5761 gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc
     5821 tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta
     5881 tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc
     5941 cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt
     6001 tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct
     6061 aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct
     6121 cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac
     6181 tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt
     6241 atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat
     6301 taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat
     6361 cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac
     6421 cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc
     6481 tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg
     6541 atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac
     6601 agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat
     6661 catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg
     6721 gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat
     6781 tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac
     6841 ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac
     6901 aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga
     6961 ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc cattgactgg
     7021 ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat
     7081 cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt
     7141 acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg
     7201 tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat
     7261 accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac
     7321 aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct
     7381 gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga
     7441 cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc
     7501 atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt
     7561 gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc
     7621 tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc
     7681 tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa
     7741 actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc
     7801 tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag
     7861 aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc
     7921 acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat
     7981 tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct
     8041 ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac
     8101 aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag
     8161 gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt
     8221 ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca
     8281 aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa
     8341 acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat
     8401 tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca
     8461 ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc
     8521 tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga
     8581 aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga
     8641 ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga
     8701 aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga
     8761 aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga
     8821 gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc
     8881 acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg
     8941 aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa
     9001 gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat
     9061 ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta
     9121 tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt
     9181 tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca
     9241 gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat
     9301 aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga
     9361 acaattacct ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg
     9421 cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct
     9481 ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt
     9541 acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat
     9601 catgaattat caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc
     9661 actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac
     9721 tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt
     9781 actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga
     9841 tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc
     9901 aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg
     9961 atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta
    10021 tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt
    10081 agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat
    10141 caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg
    10201 tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga
    10261 cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat
    10321 tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga
    10381 tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac
    10441 acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa
    10501 tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt
    10561 aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt
    10621 taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag
    10681 cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct
    10741 atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg
    10801 gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg
    10861 aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct
    10921 ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc
    10981 ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag
    11041 cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa
    11101 ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa
    11161 tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac
    11221 tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt
    11281 cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat gctctcatca
    11341 taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag
    11401 tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt
    11461 cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa
    11521 tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca
    11581 tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac
    11641 tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc
    11701 gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa
    11761 aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc
    11821 cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca
    11881 atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt
    11941 atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta
    12001 cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag
    12061 gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat
    12121 tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt
    12181 cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg
    12241 tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca
    12301 attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat
    12361 tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt
    12421 cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat
    12481 ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc
    12541 agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc
    12601 tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct
    12661 attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga
    12721 taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct
    12781 tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg
    12841 gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta
    12901 cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt
    12961 aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga
    13021 aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc
    13081 tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa
    13141 gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact
    13201 gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact
    13261 aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt
    13321 tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt
    13381 tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata
    13441 atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac
    13501 aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac
    13561 acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata
    13621 taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct
    13681 ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca
    13741 gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat
    13801 ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt
    13861 tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta
    13921 tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca
    13981 tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga
    14041 tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca
    14101 gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc
    14161 acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct
    14221 tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct
    14281 ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa
    14341 aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat
    14401 gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac
    14461 catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca
    14521 tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca
    14581 tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg
    14641 ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa
    14701 gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct
    14761 ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat
    14821 tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg
    14881 tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat
    14941 cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt
    15001 caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg
    15061 catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg
    15121 cattattata attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact
    15181 cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag
    15241 gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct
    15301 tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag
    15361 cagtatattc attactatag tccatattca tacaaccctt cattttgaat ataatcaatt
    15421 actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc
    15481 gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt
    15541 gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta
    15601 taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc
    15661 ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct
    15721 aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg
    15781 agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc
    15841 atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt
    15901 gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag
    15961 aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc
    16021 atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt
    16081 gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt
    16141 tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat
    16201 actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg
    16261 atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa
    16321 cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt
    16381 ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg
    16441 atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga
    16501 acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca
    16561 tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt
    16621 gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg
    16681 cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac
    16741 ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata
    16801 attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag
    16861 gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg
    16921 atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt
    16981 ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca
    17041 ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat
    17101 tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa
    17161 agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca
    17221 aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc
    17281 aaaaagtcgt catccgtcaa tgagacatct tggatctcat tataatggcg taatctgaaa
    17341 caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg
    17401 tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat
    17461 tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg
    17521 ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa
    17581 tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa
    17641 tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt
    17701 tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa
    17761 tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat
    17821 ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa
    17881 tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag
    17941 gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt
    18001 ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg
    18061 catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta
    18121 caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata
    18181 ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc
    18241 cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg
    18301 ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg
    18361 cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat
    18421 cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc
    18481 atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat
    18541 gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc
    18601 gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg
    18661 tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg
    18721 gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat
    18781 cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc
    18841 aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa
    18901 gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac
    18961 gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt
    19021 ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga
    19081 aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga
    19141 tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc
    19201 ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt
    19261 tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg
    19321 cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata
    19381 atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca
    19441 tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat
    19501 gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg
    19561 gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag
    19621 ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt
    19681 caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat
    19741 caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc cgaatatatt
    19801 tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg
    19861 gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac
    19921 taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta
    19981 aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg
    20041 caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc
    20101 gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa
    20161 aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta
    20221 cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta
    20281 aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt
    20341 ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat
    20401 aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg
    20461 agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat
    20521 ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat
    20581 ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg
    20641 cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa
    20701 tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg
    20761 ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga atgactggga
    20821 catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg
    20881 cacttttagg accaataaat gtgatattgc actcttcaca catggaggca aatcggctat
    20941 tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc
    21001 tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg
    21061 cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg
    21121 tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag
    21181 ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg
    21241 gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact
    21301 ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt
    21361 gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat
    21421 gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct
    21481 tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc
    21541 cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga
    21601 atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt
    21661 tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt
    21721 tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata
    21781 atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg
    21841 ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat
    21901 actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc agatatgaca
    21961 ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt
    22021 gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc
    22081 gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa aattattcct
    22141 cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg
    22201 cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat
    22261 cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg
    22321 ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc
    22381 tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct
    22441 ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta
    22501 caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag
    22561 gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt
    22621 tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac
    22681 ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg
    22741 attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt
    22801 ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc
    22861 caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac
    22921 ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg
    22981 gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa
    23041 gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat
    23101 ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat
    23161 ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac
    23221 ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg
    23281 tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc
    23341 catagccagt aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga
    23401 ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa
    23461 ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg
    23521 cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg
    23581 caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc
    23641 ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag
    23701 atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt
    23761 gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg
    23821 catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga
    23881 tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga
    23941 tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt
    24001 ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg
    24061 ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat
    24121 ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac
    24181 acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg
    24241 gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa
    24301 acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat
    24361 ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag
    24421 gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac
    24481 atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac
    24541 tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac
    24601 gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat
    24661 cactgcaacc attttcccgc ttccagctgg ggtagcttct gtcatataag cgcctctttt
    24721 tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata
    24781 ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg
    24841 ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg
    24901 ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc
    24961 aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc
    25021 accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata
    25081 tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc
    25141 cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa
    25201 ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga
    25261 tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt
    25321 tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata
    25381 ccataataat atattcctcc gatattgttt gctttttatt tttataattt aacagtctac
    25441 aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc
    25501 tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg
    25561 cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc
    25621 tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa
    25681 tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa
    25741 tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag
    25801 aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct
    25861 gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct
    25921 ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt
    25981 tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt
    26041 aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct
    26101 cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt
    26161 gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc
    26221 gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata
    26281 cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta
    26341 gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt
    26401 aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag
    26461 gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa
    26521 tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg
    26581 cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca
    26641 gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc
    26701 ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga
    26761 gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa
    26821 tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc
    26881 aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca
    26941 tttttttact atatgagctt tttttaagat tttactcatt taaaacaact gataaatcag
    27001 catttataat ttcttttatt agaaaaatca tctatctcac atattttgat tatcaaagta
    27061 ttttctgtga acagaactat agaattccct aaaacttgtc cattatttca aaatttgtta
    27121 tagtcttttt attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct
    27181 ccccatgctg ccgggtgtct atcttatgaa agaccgagat ggtaaggtca tctatgttgg
    27241 taaagcaaaa cggctcaaaa atagagtaag cagctatttt catcagaata aacagcattc
    27301 aaaaaaagta ttacgtatga ttcatcatat aacagatttt gatttcgtcg tagtagacac
    27361 ggaattagat gctttattgc tcgagtgtca attgatccaa cattaccgtc ctttttataa
    27421 ccgacaaatg aattatttca gtaattataa ttatgtacat attaccaata agggatttgt
    27481 attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata aaaaaatgcc
    27541 gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg aaattagttt
    27601 gttagctttg cgagtccagc ttcctgacct acaggaaatg acttttgaac agaaaaaaaa
    27661 ggaactccaa gggctgtttc aaggccgaaa taaaaagcta ctgacttact tgaaaaaaag
    27721 acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat
    27781 cgaattagtt acttatttta ttagacggat ccaggaacaa aagcagtttt taagaacacc
    27841 atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc actacctcgt
    27901 ttgttatggt caacttgcag aaacaatgat tacctctggt gacgtttctc ctgacttcta
    27961 ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga
    28021 tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt ttgaaattga
    28081 actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg gcttctttta
    28141 ggcatcttca acttcaaatg tcaattatcc taaaatttat tggattaagt catagatttc
    28201 catcgctacg atatcgatat tatcaaattc gtattgctga tgagtatctg tttcagttaa
    28261 ttcaaatgta tcggtcgctt tatcataagt tactgtacac ttctctacgc cttctttttc
    28321 aaaacggcga acttgcagtt cattatctgc tgactcgacc atagcctcca gtcgttttat
    28381 gattgcgact aattgtgaat gtgtcatcgg aggtcctcct tttcttattt ctgttatatt
    28441 gtatcaaaag catagcctac ttgcattgat tttctctaaa aaaactcgtt ttttatgatt
    28501 ttttttctgg tatttttcct tcaggccacc aaagtttgat caaagcttgg gttccgtcat
    28561 tctcaaaacc tttatctgcc aactcttcat atagtttcaa tgcttgggta gttgctggca
    28621 atacaagatc cattttttct gcttcttcta atgcaatctt taaatctttg ataaaatgtt
    28681 tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg aggagaataa ttgcttaaag
    28741 accagttagc tgcacttcca cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt
    28801 ttttttgtgc atagacaagc atttccgtca ttcctgtcat cgtacctgca atcatgatct
    28861 gattagccat ttttgtatgc tgacctttcc cagcagaacc atgcaatgta aatgtcgtac
    28921 caaattcttt gaataaaggc agtacttgat cataaacggc ttcttctcca ccgaccatga
    28981 tcgtcagtgt accattcttt gctcctaagt caccaccgga taccggtgca tctaacgcat
    29041 ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc caggcttggt gtacttgtag
    29101 tcaaatcaac taaaatatgt cctgagatat ctgcagaaaa aatccccgac tgtccatagt
    29161 agatttcttc tacatctttg gggtaaccta ccattgtaaa gagaatatta ctttgttctg
    29221 ctaccgcttt tggtgtatct gcccagatag ctccttctgt tactaaagga tcagcttttg
    29281 cttttgtccg attatagaca gctacttcgt gtcctgcttc tagtagatgg cgtgcgacag
    29341 cacttcccat gacgcctgtt ccgataaaac caagtttcat tttttcgacc tcctaaaaat
    29401 caaaaatcct tgcttctatg ctagtatacg ctagaaacaa ggattttttc ataaaaaatg
    29461 tttgaatttt atcttttaaa tgaatttaat tttattttga ttcgttcaat cgagtcttct
    29521 tcttcttttc ttcgcatctc ttcttcatga taaacaacca tgtcttgata aaattcgatc
    29581 atagcttcgc cgaaatgtgt agatgaaata tcataaagtt tagcttctaa aacttttgga
    29641 tctttaggaa cttcactgga aatataatca ataagtgctg gtgcaaagtc ttcgtcggtt
    29701 tcaaaagttt tccctaatga aggatgatcg aacaatcggt tcaaataagc atttccttcc
    29761 acgacacaag gtgttccggc tgccatcgct tctgtataag taagcccttg tgtttctgaa
    29821 gtagaagcac tgacaaaata atctgccgct ttataataaa gtgctacatc attgttgtcc
    29881 acttctcctg tgaactgcac aaattcttct aactgcagtt ctgagatcaa ttcctctaac
    29941 tcttctacat aaggtccttt tcctacgata actaatctgg cgttaggata agcggagatg
    30001 acctgaggaa acccattaat caaggcttgg atatttttct cataagaaat cctgcttaaa
    30061 gaaagaatca tcaaatggtt ttcctgaaga cctagtgact cccttaattt gctgatatcc
    30121 tgctgagtga tatcagaacg tttgaatttt tcaatgtcga taccagtagg aatcacgcga
    30181 agcggtgcta ttacaccata gctgcgtaat gtatcaataa cacgttcact tggacaaact
    30241 acacctgtcg tatgatttgc aaataatcta gacaagtatt ttacatggga ttgcctgatc
    30301 acttttcctt taccgatata atgaaggtag tcttcataca tcgtatggta agtatggatc
    30361 agaggtattt tcaacttctt agctaccatc ttcccaagta tccctgctcc gaattctgta
    30421 tgggtatgaa taagatctag ttctagttct ttggcaacca aataagcata ccacatgcct
    30481 ctaacaacaa ctcttcgatc cttgaaagaa ataaatggta cacttggcat tcgaatcaca
    30541 tctttttcca acttatccgc attaggatcg gtcgttgtaa aaatatagac ttcatgccca
    30601 tgtctttcta attcttgttt taatgttttg atcgatgtag caactccgct gacttgcgga
    30661 aagtaggtat cggtaaaaaa gccgattttc atgtttttcg cctccaaact agtccttgac
    30721 ctgatttact ttttttaatt tgctagagac tttttcattc agtacacttt cgtatacatt
    30781 tttaagttct tcaccgattt tattgatact tcgctctttt gctgtttgat aaccggcttg
    30841 tgtcgtactc ggtagttctt gattggtaat ctgttggatc agtcggataa attcttcgtt
    30901 tgtcttcccc atatagcagt tcttgctatc ttctaaccag ccattataca ctggaatatc
    30961 tcgaacaagt acgttttgtt tgcttgctaa agcttctaaa acaacgatcc cttctgtctc
    31021 ttcataagaa gggaaaaaga atagatctgc acctgaatac gcaccttcta tgatctctcc
    31081 acgaatgtaa ccaggaaaca ggacattttc aggatgatct tcttttacaa ttttccggat
    31141 gttccgagga atcgagtaca tgggaacatg accaaaccaa ataaatgtgt attcgggcat
    31201 cttcttagca atttctacga aatcaatgat tcctttacgt tcgaaaaaca aaccgacaca
    31261 aatgattact ttttgatccg gagacagttt gaaatattct ctgaactttt gttctttcat
    31321 tggatcaggg tgatatttct ctaagtcgat cccattggaa atagcttgaa tcggcacacg
    31381 gataccatag ctttccaaca actgttttga ataaggtgtt ggcgtgatca gatagtccgc
    31441 tttttggtac aatcctacca gatatttttt tacgatagga gaaagctggt tcgatccgat
    31501 aaaggaattt cggaaatctt cctctgtaga atgcgcatga tagacaactt tttttccttc
    31561 tcttctggct ttattgacca tattatgact attgattccg tatgtattga tgtgcaggat
    31621 gtcataatct tctttttcat ctagtgtata agagataccg acttccgtca gagcacgttt
    31681 ttgatgatca agcgcacgcc cgattccaga ttttgcaagc atcttttcac tttcaaaata
    31741 taataatacc ttcaccttac gtcccccttc acttatatag tataacatag cctacaaact
    31801 gtcactcaaa cttaagccct atttttgtct attcttactg acaaaaacgt tttcttaaaa
    31861 aagaacaaaa aaactggcgt agctctttta caagccacac cagcttttga tacgttgttt
    31921 attttacata ttcatgaaca agttcaacga cttcttccat catatcgcat tctttcaatg
    31981 cacgatcagc aagttcagcc attttcgctg tgtccaagcg tttcattaaa ctacgcgttt
    32041 taagaataga tgtcgcactc attgagaact catctagacc catacctact agcaatggaa
    32101 cagctgtttg atctccagcc atttctccac acatacctgc ccattttcct tcagcgtgtg
    32161 ctgcatcaat tacatttttg atcaaacgta aaattgatgg gttatatggt tggtacaagt
    32221 aagaaacgcg ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa tcgttcgtac
    32281 ctacagagaa gaaatctact tcttttgcaa acttgtctgc taagactgct gctgctggaa
    32341 tctcgatcat gatgccgact tggatcgtat cagatacttc cttgccttca cttatcaatt
    32401 tttgtttttc ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt gtagctacca
    32461 tagggaacat gatacgtaaa ttaccatgaa cagatgcacg aagcaatgca cgcatttgtg
    32521 tacggaacat ttcgtcgcct tgttcagata aactgatacg caatgcacga tatcctaaga
    32581 atggattcat ttcatgaggt aattgcagat atggtagttc tttatctcca ccgatgtcca
    32641 ttgtacgaac aactacaggt tttccttcca taccttctaa tacagctttg taagcttcat
    32701 attggtcatc ttctgttggg aagtctggag aatccatata caggaattca gtacggtaca
    32761 gaccaactgc ttctccacca ttattatgga caccaactag atcttttggt gttccgatgt
    32821 ttgcagctaa ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt tttaattttt
    32881 cccattctgc tttttgtgct gcgtaatctg cacctgcttt ttcaaattca gctttttgtt
    32941 catctgtagg atcgatgatt acatcgcctt cgatcccgtt gacagccaaa atatcgcctt
    33001 ctttgacttt agcagtaatt tcttttgtcc caacgattgc tggaatttct aaagaacgtg
    33061 ccatgatagc agaatgtgaa gtacgtccac caatgtctgt tacaaacgct tttacaaaat
    33121 tgcgatctaa ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg accacttctt
    33181 cgttgatcat tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa atacgtttag
    33241 caacgtcacg aatatctgca gcacgctctt gcatataagc attatcttcc attgcttcga
    33301 acataccgat atacatatcc gttacttctt ttagcgctga ttcagcattt acgctgttgt
    33361 ctttaatatt ttgcttgatt tgacctacca tttctggatc tgaaagaacc atcaaatggg
    33421 catcaaatac ttgcgcttct gcttcaccta agctttgcgc tgctttttca cgaatttgct
    33481 gcaattcttc agtagattta gctaatgcag catccaaacg agcttcttca gccgcagtat
    33541 cttctactgt tgccttactg aatgttaaat ccggttgaac tagcaggtaa gcttttgcaa
    33601 cggctacacc gtcactagcg gcaatccctt ttagcatttc aaccattatt cagataagcc
    33661 ttctttcttc attgtttcaa cgattgctgc cattgcatca gcttcgtcag caccttctgc
    33721 agtaatagta acatcagaac cttgacctac acctaaagac ataacgccca tgattgattt
    33781 caagtttact gatttacctt tatactctaa gtttacatca gagttaaatt tgcttgctgt
    33841 ttgtactaat agtgtagctg gacgagcgtg gatcccagtt tctgctacta cgtgaaattc
    33901 tttcttttcc atattaatcg gtctccttta agtgagtgtt gtttttatgt gagggttttc
    33961 ttttttagta attcagagaa tcctaacaaa tgccaccctt tcatctgtaa agattaacat
    34021 tttttttcgt tagatacaac agttttcatt caatttcaga taaaagtatt caaattattc
    34081 cggattcttt ttcatccttt tcttcacatt ttataacaaa cgtgtttatt tcctttttct
    34141 tgtcgctttc tcttctcctg acgtttatca ttctttcctt tgaccctctt tgttttcata
    34201 tgaaacgaat tacttgcttc cctttttatt tttagtataa taagagtcaa aggtcaggaa
    34261 tggtcaatag agatttcacc ttttctacct cataaatctc ttaaaaaact aggggttatt
    34321 cttttacctc atgaaaaacg agatgtaaaa tatggatgta cccatttttg tgaaaggacg
    34381 tgaatgattc atgctttgtc aaaactgcgg caagaatgag gcaacgattc acttgtatgc
    34441 cactgtcaat ggtcagcgga cacaactcga ttactgtcag agctgttatc aaaaaatcaa
    34501 aaatcaacaa aatggaggtc tgactaatat ggcccaaaat gatccatttg gatttggcag
    34561 tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca
    34621 aacaccacca acacaatttg gcggaaacaa caacttcaat ggtggtcagc cacctcaagg
    34681 caatacagaa ggtttattgg gagaatacgg aatcaatatc acagaacaag cacgacaagg
    34741 tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa
    34801 tcgccgaacc aagaacaatc ctgtcttgat tggagaacca ggtgttggta aaacagcagt
    34861 tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa
    34921 agaagtcatt cgtttagatg tcgtatcttt agtccaaggt actggtatac gaggacaatt
    34981 tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt
    35041 catagatgaa gttcacgaaa tcgtcggtgc tggttctgct ggtgatggaa atatggatgc
    35101 tggtaatatt ttaaaaccag ctttagctcg aggagaattg caaatggttg gtgctactac
    35161 cttaaatgag taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt
    35221 acgggtagat gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg
    35281 ttatgaagac tatcatcatg taaaatatac tgatgaggcc atcgaagcag ccgcaacttt
    35341 atccaatcgt tacgtccaag atcgcttctt gccggataaa gccatcgatt tgctagatga
    35401 aacaggttct aagaaaaact tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa
    35461 actgcaagaa gcagaagaac aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc
    35521 agcttattat cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc agctcactga
    35581 agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg
    35641 tattccagtg ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact tagcagatga
    35701 cttgaaagct catgtcatcg gacaagataa cgctgtagat cgagtagcga aagcgattcg
    35761 ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc ggctccttct tatttgttgg
    35821 accaactggt gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac tattcggttc
    35881 acaagattca atgattcgtt tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa
    35941 attgattggt tctcctccag gttatgtcgg atacgaagaa gctggtcaat taacagaaaa
    36001 agtacggaga aatccttaca gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga
    36061 tgttttgcat atgttcttgc aaatcttaga tgatggtcga ttgacagatg cacaaggaag
    36121 aacggtcagc tttaaagata cgatcatcat tatgacaagt aatgccggaa ctggtaaagt
    36181 agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt actcgttctg tattgaacca
    36241 attaaacaac tactttacac cagaatttct gaaccgtttt gacgggatca tcgaattcag
    36301 tgcattatca aaagaaaact tgatgacaat cgtcacacta atgctagacg atgtcaacca
    36361 aatgctggca gcacagcaac ttcacatcga agttcctacg aatgtcaaag aaaaattagt
    36421 tgacttaggc tatgatccat ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca
    36481 aatcgaagat ggtatcgctg aattttatct tgatcaccct tctatccatg agttgaaagc
    36541 aaaactggat aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga
    36601 atcagccgaa gaaacggctg aataaatcca gttttatagc gtgagacggt atttctttga
    36661 gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga
    36721 aattataata agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt
    36781 ggtaaatgta acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc
    36841 ccatctagtc aaggtctaca cagccggaaa taccactatt gtctacactg aagcccctga
    36901 acataatgaa attctgctga tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga
    36961 tgctaaaaat tatttcttag ataaaatcaa agacgccgtt taccacgagg atgaaatcaa
    37021 agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat taggcgctac
    37081 attagccgtg taaacaaatt ttgaacataa taattaaaga gcgtgggaca aaagtaaaga
    37141 acacttttgt ctcacgctct aaacacgtat aaacggcgaa agcagaagca actccttcgg
    37201 aaataagctg aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct
    37261 tggagttaaa cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt
    37321 tcttttgtac ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt
    37381 cctctttatg gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt
    37441 ttacatctaa taatcgacat aatcgtaaca tctcttcttt tttctgtttt tttctttcac
    37501 gtctttgcca ttctgtttcc atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa
    37561 ataattttta tatttttttg attagacatt tctatgattt taatatagta aaaagagccg
    37621 tcacggaaaa tccgtcacgg ctctaaacaa aatatgagag gctgtttttg tttggctatt
    37681 cttattactt gatgcagggc agcttgtgtc aagacttctt tacatcaaac tttttaattt
    37741 tacattggga tatttatccg caaaccagcg ttctgcaaat tggttctcga ataagaacaa
    37801 cggttggtcg aaacggtcac gagccaagat attgcgactt gaactcatct tctcatccaa
    37861 atcttctgga tcgatccagc gagcgatttt gtgtcccatt ggagacatga ttacttcggc
    37921 gttatactca tttaacatac gatattggaa tacttcgaat tgtagctgac caacagctcc
    37981 aatgatatat tcttccgtta gatatgtttt ataaagctga atcgctcctt cttgaactaa
    38041 ttgatagatt cctttgtgga aggatttttg tttcatgaca ttttttgcag tcactttcat
    38101 aaacagctct ggagtaaatg aagggagttc ttcataagca acttttagtt ttccttcgaa
    38161 caatgtatcc ccaatctgat aattgcctgt atcatataca ccgatgatat cgcctgctac
    38221 tgctttttcg acgttttctc tcgaatctgc cataaattgt gtgacattgc ttaatttcag
    38281 ttttttattt gtacgttcca accagacatc cattcctcgt tcgaatgttc cagaacagat
    38341 tcggacaaaa gcgattcggt cacgatgggc tggattcata tttgcttgga tcttgaatac
    38401 gaaacctgaa aattcttcct cataaggact taccatctga tcatcttgtg ttttgtgtcc
    38461 ataaggagct ggtgccaatt caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt
    38521 caatgcagaa ccaaaaaata ctggtgtctg atcgccacga gcaatttttt cttgattaaa
    38581 tgcatctcca gcctctttga ttagttctac ttcttctaag acttgttcat ataaggaatt
    38641 ttcagttaga ggatgatttt cgggaagcag tccatcttca tccaattcta ctaatcgttc
    38701 atcctgataa ttttccggac gataaaactc tacacgatta tggtagatat cataaagacc
    38761 ttctagccct ttacccatac cgattggcca attcatcgga taagattcga tatctaatag
    38821 ctcttctaat tcttctaaca attccaatgg ttcacgacca tcacgatcta gtttgttgat
    38881 aaaagtaaat atcgggatgc cacgtttttt tacaacttgg aataatttct ttgtctgtgc
    38941 ttcgatccct tttgcactat cgattaccat gaccgcacta tccacggcca tcaaagtacg
    39001 ataagtatct tcagaaaaat cttcatgtcc tggtgtatcc aagatattta ctcgtttccc
    39061 ttgataatca aactgcataa cagaactagt aacagaaatc ccacgttgtt tctcgatttc
    39121 catccagtct gatttggcaa aattacctgt ttttttgcct tttacggtcc cggcttgacg
    39181 gatagcccca ccaaacaata gtaattgttc tgtaatcgtt gttttcccag catccggatg
    39241 ggaaatgatc gcaaaagttc tgcgattgtc cacttgttgc tttaattttg gattattcat
    39301 aacctaactc catttcttcc attcaattat ttatattttc tctaggcaca gattgtccga
    39361 tctcttaaag aaagacaatt tcatataggg ctgtgacaaa agccgtgtag cagtctcttt
    39421 ttccgagtaa acggcgttct agagacagcc tatcagcaat aagcccaccc aagccaaaaa
    39481 tacgaaaagc tgtttttgct tggctgctct tattacttaa tgcaggatag ctttttcaca
    39541 acctttgtca actactacaa ctgaatcagt atagcatgga ttcagcgaat gaaaaagcca
    39601 ccaaaccata agagtcaaat atttttttgt cagctgacta aaagaaaaga cgtgttgctt
    39661 gtttattcaa gcaacacgtc tttcataacc aagtttccaa ttatcttctt ggctcgtcat
    39721 cttcgaattc ttttctgaag tatcttcgtt cttcttcagg ttccgcttct gtttcttccg
    39781 gatcatgaaa aatgacacgg attttaagga ttcgagaacc ttccatttct tcagaaacca
    39841 gcgtaatatt atcgacatca aacgataact tttcgccttc atccggaatc atgcctaatg
    39901 ctgtaatcaa ataaccagcc atcgtatcta catcactcat atgcagatca ctgtcaaaag
    39961 cttcattgaa ctcatcaatc agcatacgtc cttggatgat atattcgtgt tcatcgattt
    40021 tttcataaag attctcaact tcatctgatt catcatcgat ttctccgacg atttcttcta
    40081 aaaggtcttc tagtgttgct aacccaacga cgccgccata ttcatctaat aaaatagcca
    40141 tctgattctg cgtctttttc atttcatata acagatcatc aataaagacc gtttcaggaa
    40201 cgaataaagg ctcctgcatg attttcttga tatctaaatt atcaaagccg aatttatggg
    40261 cagcttttag taaattcttt gtatgaagga ttccgactac tttatctttg tcttcgttat
    40321 aaacagggat tcttgaatag ttttcggata aaacttcatt cacattttct tccacagtgt
    40381 cgttgatatc aatcataaat gcatccgttc gtgggaccat gacttctcgg gcaactttcg
    40441 tatcaagaga aaaaacccct tggagcattt ctaattcttc attattcaat acgccttcgt
    40501 tttcaagcat ataacgcatt tcatctctgg tcatttttga atcttcatca tcaaaagtca
    40561 tcggcgtgat acgcgccaat aaacttgtgg aagcagaaag cagccagacg aagggcttag
    40621 ctactacacc aatcaagcgt atcagccctg aagtgaactt tgcgacttct tctgatttat
    40681 ttaaagcaat acgtttaggg tacaattctc caaaaacaat cgatacatag gtcaaaatag
    40741 ccagcacaat gatatttgca atattttttg ccgctgtgcc gccacctaat accggagcta
    40801 accgttccga taaagtatta gctagtgaag caccagacaa aatgttgacc aaagtgattc
    40861 ctacttggat tgtcgatagg aaattatttg gattctgcaa tatttttaaa agcttttgag
    40921 atttcacatc tccttcatca gctttttgct cgattcgatt cttattgatc gaaacaacag
    40981 caatttcgga agctgctaaa aaagcattga tcaatgtcaa aacgatcaat aataaaatct
    41041 gcgctaacag cgactgactc tcagggtcag cattcataat cgcattctct ccttataaaa
    41101 ttaattttta gtttacgata gaaaaaaacg tggcactata gccacgttaa aatggtatca
    41161 caaagcaatt ttctttgcaa taaaaacaag tgtactaaaa ctacctacga ggtcgtagca
    41221 attttcttct tgttttcatt ttgcttgtct tctttgacga ttttgattat ataggaaatg
    41281 atcgtcttgc agattgcgta aaaaggaaca cctaagaaga taccaagcaa tccggcaata
    41341 tttccagcta ctagaagaac gataatgatc gttaatggat ggattttcag tgatttccca
    41401 attacattag gataaatgat attcccatcc aactgttgga cgattagcac aaccacacaa
    41461 caaagtaatg cttttaccgg atcattgaag actgttacta gaaatgcagg tgctagtcct
    41521 agataaggtc ctaaatatgg aattaggttt gtcaatcccg caatgacccc aaataagaaa
    41581 gcataatcca caccaattac catatatcct aaaaatgtga aggtaccaac gaataaacat
    41641 tcgattgctt gtccgctaat gtaattagcc aatgttttat tcagttgccc caataactcc
    41701 acaatttggt ttcttctttt ttctgggaaa aactgttgga tgtttggcac tagtttttct
    41761 ccgtctttca acatatagaa caagatgaat ggtgctgtaa tgattactac ggttgccgaa
    41821 gcgatcgttc caacgatcga acctaaacta ttggatactc cactaaggaa ttgctgaatg
    41881 attgtcccgt aagaaatgtc taattgctca aaatatttat tgatatctac ttccttgaac
    41941 aaaggcaatt cggctacctc ttttaaccag ctttctacct gtttgatgaa atcaggcata
    42001 ttagatgcca atgaggaaat ttgctgtact agacttggga tgacactaag taaaatccaa
    42061 ataattgctg caactaaaag gagcagcaca attgcgacag cccatattct tttgattttt
    42121 gctttcatca acagcttgac aataggattc aataaatagt aaagaaagcc tgcaatcagt
    42181 acaggtgcaa ataacgtgga aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct
    42241 gatgaaatca tgatcaatgc agctatgatc aataattcaa gcgaccaaaa cattagttta
    42301 gactgtctaa gtttttcaaa caatagattt cctcctttct ttcttactag tatatattat
    42361 attcgttaaa aattctttta ttcttttttt atttttgaaa acttgccata ctttttattt
    42421 gttttatttt ccagtatact atacgaaagg aaggtggcta gaccatgaaa gttgtacaaa
    42481 caaaagatac aatgagcaat atttacttag atgctgtacg gatcaggcat caagtttttg
    42541 tagtcgaaca gggtgtgcct ttgtctcgag aaatagacaa agatgaagcc cattgtatcc
    42601 acttcgtcct gtactcagat aaaaaagaac cgcaaggaac tgtacgtctt ttgcctttag
    42661 aaaacggaaa aatgaaatta cagcggatgg cgattctttc tgaatacaga caccaagggt
    42721 taggaaaaat attgattgaa gaagcagaaa actttgctaa gaatcaaggc tataatacga
    42781 ttttattagg ggcacagtcc acagctgaaa ctttttacga aaaactagga tatacagctt
    42841 acggcgatcc ttttgaagat gctggcatgc ctcatattta tatgaaaaag actttataaa
    42901 aatttatttc gaaaaaagca aaaaacagca agcttttgag aaaaatctct cactggctta
    42961 ctgttctttt ttgtgtttac ttacgcgaca attgcttgta acggttatac caaatatcaa
    43021 tatagccttc cgagaacggg ccatctccat tattgatcca gtcaaccaga atcttcacat
    43081 tttcttttag gatgaagtca atatcgttcg ggtaattcat cattttgctg tgcatctcat
    43141 attcatcgac atccaatagt cgtttctcgc cgtcaggaaa taccttgata tccaagtcat
    43201 agtcgatata tttcaaggcc tcatcgtcca gcaaaaaagg tgaagctaga ttacaataat
    43261 aggaaccccc cttttctctg atcattgcta ttacattgaa ccagtatttt ttatgaaaat
    43321 aaacaatcgc tggttcgcgc gttacccatc ggcgtccatc agattcagtg actaatgtgt
    43381 gatcattcac gccgatcata gagtgttcgc ttgtttttaa taccatggta tctcgccacg
    43441 ttcgatgcaa tttgccgtcg tgcttgtaac tttggatcgt cacaaactct ccttcttttg
    43501 gaactcccat agcaaccctt ccttaagcag cattttgtgc tttgtgtttg ggtgttcatt
    43561 acaccgacat cataattatt atatcaataa aaatgagctt tgcctagaaa ggattcctat
    43621 tgtttcgatt tttctctaac tgatctacca gtttcatttg gaccttagga aaaacgaccg
    43681 aatcaaatgc atcaggcttc aaccacttcg aagttttgtt ctcggtaaat tcttgttctg
    43741 ctccctcggt tgcccgtccg taaaaaagga gaacatgcca tttcagatga ctaaaaatat
    43801 gagtaacttc tcctaaatgt cgtgtctgcc aaacaaagag ttcctgcttt tcaaatggga
    43861 gattttgctc ttcttctgct actaggtcat caaacagatc gatttctggc tttgtttccc
    43921 attctttttt caatcgctca tactcctctt gtgtcacttc catcattgga aatgtccaca
    43981 tattcgccag caatttttgg ctatctcttt cttcaaaata atacgctcca gaatgatttt
    44041 gaagagctgc actgatataa taaacgtctt ttggctttgc tttttttgtt tttacaggaa
    44101 aactcgtttg gattcctcgt ttgttagcta aacaaaaggc ttggatcgga caagtctcac
    44161 actttggaga agttggtgta cagatagctg aacctagatc catcattgct tggttgaatt
    44221 cacctggatg cttttcatca atgattttcc gcatcgcttc atcaaagatt ttcctgctag
    44281 aagcttttgc gatgtctgct tcaatgcaaa acaatctgct gactactcgc atcacgttgc
    44341 cgtcaactgc aggttctgga agtccaaatg cgatacttgc aatcgcgcct gtcgtatatg
    44401 gtcctattcc cttcaatgag ctaatttctt caggcgtttg cggcatttcc ccatcaaatt
    44461 ctgacatgat ctgtttagca gcagcttgga tgttccgggc tcttgagtaa taaccaaggc
    44521 cctcccatgc ttttaaaagc ttttcttctg gagcatttgc tagctcttca attgtaggaa
    44581 accattccat gaaacgataa aaataatcga tcactgtgtc tactctggtt tgctgaagca
    44641 tgatctcaga gatccagata cgataaggat cacgattata gcgccaaggc aggtttcttt
    44701 tttcttgttc ataccattga atgaactgat cttgaaattc ttttgtttct ttatctgtcc
    44761 atttttccca ttctttattc actttcctct tccttttccg taatgaatcg ctggatcata
    44821 tccaaatcaa atcctttttg atataagctt tgctttactt ttagatttct ttttttaggt
    44881 tcaaacctta aatttcttcg ccatagttta tctccttgtt tgactaaata atcatattct
    44941 atttcttgat ctgcttcttg cggaagatca gctatcgctt gttggatcac ttcttgagaa
    45001 aagcctttcg tcatcaatgt ttgttggatt tttctcaata attctttttg gctttttccg
    45061 tgattttttc caaacgcttt ttcggctgtc tgatgtgcat tttgaacttg gtcttcaaat
    45121 gtatattggc tgagcgcttg ttcaatgatt tcaggtttca aacctttttg ctgcatctgc
    45181 tgcgctaatt tctttggtcc tttgtcactt aagcgcatat tcgtgcgcac ataactttcc
    45241 ccatagacca aatcatcgac aaggtccaat tcttttaagc gggctacgat tttatttcga
    45301 tcttcttgaa ggatttcctt ttcttttaag tatgttcgga tttctttttc cgatcgcaac
    45361 tgataactta agtaattcat tgctaggtga aaaccgaaat cagctccgct actcttcttg
    45421 atctcttgga tagtttcttc gtctaattcc cgtcctttta ataatcgata gcgtactaat
    45481 acatcttcag atacttgtaa agcttctcct tcagataaat ctacgcgata gaaagggcct
    45541 ttgcctttac tgattctcgt aacggttaac ataaacatcc tcctcgaaca tatattctct
    45601 atcataccac agaaaatccc cgtaatcagg tattatatgt tagaataaac cacagaaaaa
    45661 agaaaaaggg agatacttat ggcagaaaca attgtcaaaa ctggacaaac ggttcctttg
    45721 aaaatcaagc gtttaggcat aaatggtgaa ggaatcggtt atttcaaacg gttgatcata
    45781 tttgttccat atgcactacc aaaagaagaa gtactagtga aaatcacgaa agcaactcct
    45841 cgctatgcag aaggagaatt aataaaagtc aaaaaaacaa gcaaagatcg cgtagtggca
    45901 ccatgtcctg tttactatga atgcggtggc tgtcaattac aacacttagc ttatcaagct
    45961 caattggatt ttaaaaagga tctgcttttg caagcattgg aaaaattcaa accagcaggc
    46021 tttcgatctt atcaactttt gccgacgatc gggatggatg agccttggca ttatagaaac
    46081 aaagcacaat ttcagttacg aaaaaataag cagactcaaa aaattgaagc tggtttatac
    46141 gaagcaaact ctcatcagtt agtccctata acagactgct tggttcaaga acctcgaaca
    46201 caaaaagtaa tgaatactgt tgttcaacta ttgaataaat ttgatgcacc catctatgat
    46261 gaacggacaa acagtggaat cttccgcaca atcatggtta gaatcggtgt taaaactggg
    46321 gagttacaag ttgtttttat tactcgcagt aaaaaattcc cgcaaaaaaa cgcaatgatc
    46381 cgtgagatca atgaacgact gcccgaagtt gtttcaatca tgcaaaatgt ccaggcacaa
    46441 aaaacgtctc tcgtgatggg ggatgaaacg atccacttat ggggaaaaga agccattgaa
    46501 gaacaaatca atgaagtgac tttcgatctt tctcctcgtg ccttctttca actcaatcct
    46561 cagcagaccg aattgcttta tggcgaagga atcaaagcat tagatgtcca gcctaatgaa
    46621 acgatcgtgg atgcttattg cggagttggg acgatcggct taagtgtagc aaaacaagcc
    46681 aaagaagtac ggggaatgga taccgttcct caagcaatcg ctgatgcaaa aaaaaatgcg
    46741 gaaagattag gatatcacaa cactcgatat gaagtcggga cagcagagca agttctgcct
    46801 aaatggttga atgaaggttt tcagcctgat ggtatcatcg tagatccacc acgtacgggt
    46861 ttggataacg cattgatcca atcattattg aaataccctc caaaaaaact tgtttatatc
    46921 tcctgtaatg tttctacact tgccagagat ctggttaatt taagtaaaaa gttcaagatt
    46981 gaatatttac agtccgtcga catgtttcct cagactgcac gttgtgaagt tgtcgtgaag
    47041 atgacgagac tcgctgacta aatgaaagag gttgtgaaaa agtctgccct gtatcaagta
    47101 ataagaacag ccaaacaaaa atagctcttc atgttttatg aatactgtac ttgtgaccga
    47161 agagttaaca ggaggacact atttatttaa aaaaagggtt gtgacatagt ttttgtcaca
    47221 gccctttttg attattattg gatattcacg tctatcactt gataaaatgc atttgtcgta
    47281 tcgctgatat tccatactgc taaaatcaca ttatatcctt ttcgatcctg cggaagtgtg
    47341 atcgtttggt tgactgtttt tggtggaaca gttgctttgt cgtctatttt tgtgatcaat
    47401 tcaaaatcac taaatttcaa aggttgatta ggattccaag ttggtttggt aatgtagtaa
    47461 tcccaagtac ttgtccgatg ttgtgctgtt aaattccaag taatatttaa tgttcctgga
    47521 ttaaccatgt ttttatgcca tctgttagat gtctgttcat ctaaaggctc aaagccgctg
    47581 actcctgcac tagcaatttt tccatcaatg aatgtatttt tggtagcttc gatactttgc
    47641 ggttcccatt gtgctcttcc gacattttga tttaagtttc ctgctgctga tgttcctagg
    47701 tatgcacggc ttcctggaga agtaatataa ccatgcgcaa aacctggaat gctaaaaccg
    47761 aacaacccta aactaactaa cccaataaac aataacgtac taatcttttt catgtagaaa
    47821 cctccttcat ttgataacca cattgtaaac gctttattgt attaacacaa acattatgct
    47881 tttataattt tatgttgaag taaaaaaggt gaaaaaagta agatgcagat tataacagaa
    47941 gtagacgaga aaagtacaaa tagaaaacat gagaatcaaa tcatatgatc tttttcaaaa
    48001 acatcattca tataaacaaa tgttcaactt tttaataaca acaattatca atttttttct
    48061 tacttcacaa actaaataca ggtcttgctg tgagccttca tggtaaacta atattataga
    48121 aaggagttag taaaatgaag atgataaaag atattcgttt ttgggtttgt gttataggaa
    48181 ttgtcatatt aggcttttta tcagggttgc ttagcggaaa tcccggaaaa tattactatt
    48241 ctttgcaact tccaccattt gcaccaccta gctggatatt tgggccgatg tggaccttac
    48301 tgtatatatt gatgggtatt tctctttatt tgttacttaa tcacaacaac aaaaaacagc
    48361 gaaacaatct tgtcggtctt tttgtgattc agttcatttt taattttata tggtctgctc
    48421 tattttttaa tttaagaaat attttcattg cagcaatcga tattacactt ttagttattt
    48481 tcttgtcagt gcttatgtat cagttgtggc tgcatcatcg cctggcaatg tggctcatga
    48541 ttccttatta tttatgggta ctattcgcaa ctctattgaa ttattcaatt tattttttaa
    48601 actaagcgct aaaaagagac gtcctgtaat ttaacacata gaaaaagtgc cacctaagtt
    48661 agattttttg tctaatttag gtggcactct ttattaacca aactattttt ttctagtacc
    48721 cacttgttgt tccatactca ttattctggt cataaccata accattttgt tcctgtccag
    48781 cgtaaggatc ataactttga gtatcttgac ttggtgtata gtactgactt gtatcttctg
    48841 tttgctgata ataggtattc ggatcttggt actgatcata tgtgttttct gaataggaag
    48901 aataatcggt gtacccttca ctattcgatc catcaccgtt tgtcgaatcg ccatcttccg
    48961 aatcatcaaa gaacatcatt gaagcattct catttttcaa attaggtaat gtatcgcttg
    49021 tttttatatt taattgtttc ttcagttcat tttggatcga aagaagatca ttttttccta
    49081 aaatttgata gtagatatca ttgatcattt gtccttcgcc ttgcaactgg tcttgtttga
    49141 ttgtttcaaa cgccggggtg tagttcgtcg ctacatcaag catgtcatcc caatctaagt
    49201 ccgtcgtcac attgttctca actgctttta aaatttttct ataattgctt acgccatcta
    49261 aacttaaaac tttattgacg attttcgtca ctacttcccg ctgacgacgt tgtcgaccga
    49321 tatcgccttc tggatcttcg tgtcgcattc gagaataggc taaacctttt tctccattca
    49381 atgtttgttc acctactggt acatggacac catccaaagt aaagtcaatt ttattattca
    49441 ctttgatgcc accgaccgca ttgatcaaat cttccatgcc atccaaattg atcgttacat
    49501 aatgatcaat aggaatatcg agcagttgct caattgaatc catggccatt tcgacaccac
    49561 caaatgcgta ggcatgattt aatttatcta ccgttccata acctacgata ttcgtataaa
    49621 tatctcggtc caaactgatg atcgttgatt ttttttgttt cggattaact gttactacca
    49681 tcatcgtatc agaacgtcct tgttccgttc ttcctaatcc accggtatct aaacctaata
    49741 gcaaaaccga aaaaggttct ttgtcatcaa tgcttactct ctcagaacgt ttggaggata
    49801 ttcgattaga gtttttagaa attttgttta ctgtttggct cgcttctccc ataaatctga
    49861 ttccgtatgc tgataatccg gcaatcagca aaacgaatac ccctaacaag gttaatagaa
    49921 ctttttgcca tcgcttcata atacacctct gtttcacatt ttctaaaata ttgtctcata
    49981 taactgttca atcagcaaat tcacttttta atcataagaa aactttaaaa aacatttttg
    50041 tgtaacgatt attatttttt aaaatttctc aaaattctta actatcatac cataccgtga
    50101 aaaagatcga aagaccgaat acacgcagtt cgcaatcgtt ttatcttttt ttatgtattt
    50161 tttccagaaa aagcgaacaa caaggtattt tccaaataat cgtgcagtaa aatgatcgca
    50221 catacctttc ccatcttttt aacagtcttt gcctaccgtt cagattatca ttcgatttca
    50281 cgacaatttc ttcctatgac tggtcctttt gttaaaccca tcttctattc gttttatttc
    50341 cagctcactg catggctctt aaaatgactc gaactagtga ttgcattacc atcatctttt
    50401 aatggataga tatgctcctc ttcaaagtaa aacgattttc ttttaaagta atagaattga
    50461 catggtaatg agtatgtata ctagtggttt cacaaatggt cttttattca acatccaaaa
    50521 actgaaattt ctttaaaatc cattactaac gaagaaaatg ttccttgctt gataattagt
    50581 taggacatgt caagtgaaca aatgcattta cttagccaaa aaatctaaaa acttcataaa
    50641 tagacaaaaa gacacgactc tctaagaagt cgtgtcttta ttcaacaaat tattataatt
    50701 tagtcacttc agcagcttga ggtccgcgtg caccttcaac aatcgtgaat tctacttctt
    50761 gaccttcttc taggctcttg aagccgtctc cttgaattgc tgagaaatga acaaatacgt
    50821 catctccgcc gtctactgta ataaacccaa atcctttttc attgttgaac catttaaccg
    50881 ttccgtgttc cataaatgta tatcctccaa aaaagttctt gcaagtaacc ttgcaagaac
    50941 attataccca atttagaaaa cgctagcaag ctttcgctct tttattttcc aaattatttt
    51001 taagactgat atgacggagg aaatggttat tttgctgctt cgaaacgttt gtttacttca
    51061 tcccaattaa taacattcca gaatgctgca atataatctg gtcggacatt tttatatttc
    51121 aaataataag catgttccca aacgtccaaa cctagaattg gtgtctttcc ttccatcaat
    51181 ggagaatctt gatttgcagt agaggtaata gctaattttc cattttccat tacaagccaa
    51241 gcccatccag aaccaaatcg tccagcggct gcttttttga attcttcttt aaaagaagaa
    51301 aaatcaccaa aagcttcatt aatcgcttct tttatttctc ctgtaggttc gccaccagca
    51361 tttggtgcca tgatttccca gaaaaatgaa tggttagcat gtccgccacc attgttacgt
    51421 acagctgtct tgatatctgt tggaatagcg tccatatcag ataataattc ttctattgtt
    51481 ttttcgccta attctgggta tttctcaatt gctgaattta aattcgtcac ataagtattg
    51541 tgatgcttat catgatgcag atgcatcgtt tcttcgtcaa tataaggttc caatgcgtca
    51601 tatgcataag gtagatctgg taatgtgtaa gtcataaaaa aattcctcct caatgttttc
    51661 ttaataatgt ctacattaca aagagtaaca aagagaagga aattcttcaa ttgttatgcc
    51721 tagtcgtctt tttcactttg acctacttct tcttcaaaaa cctcagtttc tttaaaaata
    51781 aacaacttgc tgaaaatata gttcgcaagg ataacaataa tttgagtgat gattttcgcc
    51841 acatactcat tgctgttcat aacattgatc aacaatacca ttgcccccat gtccaatccg
    51901 taagaaagga tacggtagaa gaagaactta ccgcattcaa taagtgcatg tgtaaaattc
    51961 ggtgattttg aatggaatac ccaaagtttg ttcgttacaa aagcaaacaa tacagaacaa
    52021 aaccacgaaa tagaattgga taaggccata cttaaatcaa aatattggcg tgcgagagca
    52081 aaactaacaa aatttacaat cgtagccaaa ccaccaaaaa agagatagac tagtatctcc
    52141 caatatcctt ttgcttctaa atatcctttt aactgtcgat actgcttcat gagccagcct
    52201 accttattaa aaattcttat ttatcatact ctatatttca ataactttca tctttattta
    52261 aaaaaattaa ttttttttga tcctttgcct ttgtctctgc ttggatcgtc acatgtgtaa
    52321 taccatattt taattttaat aaagaaatta tacgaggata tatcttttca atctcgctta
    52381 gtagctgatc ttcaaaatca atatgtccgt cgaaaataat tatcttttcg ctatactgcc
    52441 aaatatggac atgatgaaca tcctttaccc catcgattgc ttcaatttct tgttccattt
    52501 tttcatagtc gatagcaggt gcactttgta tcaaaatagc ggcagctttt ttgatcacca
    52561 ttatcgcttc tatcaatata tatatcgaaa tgatcagtgt aataactgga tcgatccagt
    52621 agatatcgaa aaaacggatc aaaattgctc cgataatcac acttaccgaa gacagtgtat
    52681 cacttaacaa atgcaaataa ctagatttta tattcaaact ttcatctgct tcttgtcgta
    52741 ataataatac tgagaacaaa ttcgcgagca atccaataac tgctactgtt agcatcaaat
    52801 gactatttat cttttctggt gaagtgaacc gcttgaatgc ttcaacggtt aaaaacaagg
    52861 ataatatgat caatatgaat gagttgatga aagcactcaa tatttctaaa cgctggtagc
    52921 catacgtacg ttttttattt gcttcttttc gggccatttt ttgtgccccg taagaaaaaa
    52981 taattgccaa tgtatcacta aggttatgga aagcatctga aacaagagac aaactccctg
    53041 acataatccc tccaataaat tctgataaag tgatcaccgc attcaaaacc actactccaa
    53101 atagtttttt tgatgatctc tgtggctgtg taggcactat tccttcctcc attttattta
    53161 tattggatag tctttagaca agaataacat ttttttgata tttcaccaat cattcagctg
    53221 tttccttaaa gcacttgtat ttcacttgat acttcgctag aattgcaagt gatgacacta
    53281 ctagatgagg agtacttatg acaacaaaac aattaattat cagttccttt atacgattca
    53341 aagacctaaa ggaggcgaga aatgtcccat tttggaaagc cattgtctat cttttagttt
    53401 tgagtataat tatggctttg ccaatctctt ttcaagtttt tcaagtctta aacaacattc
    53461 aattggatgg gcaaaagatt gctactcgca ttcctgattt tacaatccaa aacgggcagc
    53521 ttcaaaccga agaaaaagaa ggattcattt accaaacaaa ctccattatc ttcacatttg
    53581 atccagaggg aaaacgaaca gaacaggaca tctctactga tttgatgggg aattttgtta
    53641 gtgtcgggat gttaaaaaac aaacttatca tagctcttcc taatacaggc acgacttctg
    53701 ctttgttgaa caataaccaa ttagaacttc cctacaccaa tgaatcttta aaaaacttaa
    53761 caggtaagca gctccgttct tttttaagtg aagcatctat accactttgg ataaaagtcc
    53821 tgacatttat attttctatt tatcctagtt ttctgaatct gctgatcact ttgctattta
    53881 ccaacattgc tgcttttctc tatgcacgct ttcgactaac aaaagctact tttcttgatt
    53941 gtttaaaaac attgatctat tcaatttcat tgcctactat aattgcaaca atcttgatgg
    54001 tctttcttcc ttcttttgac actagcgcct tcattgcttt tgcaggaatc tttatttttg
    54061 ctcaagctgt caaaggatgg gcaaaaatat cgatcaatta agaaataaag acaactcgtt
    54121 atttctactt caatagaccc ttttagaggt tgtgacattt ttttgtcaca gccccttttt
    54181 tatttgaaaa atatttcttt ctcaaaaaac aagcaaggcg cttcaaagtt cttcttgtat
    54241 gcgctacact tagagtaaag aaaggcggga aattaactat gtcagaaaga atcatttatg
    54301 cagatcgtcc atttcgtgat gaatttatcg aagaaatcca tactatagcg cctggttata
    54361 tctttaaaac agaattacaa ccagaggatc tacaaaatgt agagatcagt ttgggctgga
    54421 actcagacta ccaagaaaaa ctattagcct cttctacctt gaaatgggtc caatctatct
    54481 cagctggtgt tgatcagcta ccccttgaga ctttttcaaa gaaacatatt ttactttcaa
    54541 atggtagtgg catccacagt gaatcgatta cggagcatgt cttaggaatc atccttgggt
    54601 attctcgtgg tctttttcaa gcacaaaagg cacagagtga catgaaatgg ttaggttcat
    54661 cggtccatta ccaatctatc caagggaaaa aaatactaat cattggaact ggccatattg
    54721 gaaaaatgat tgcaaaaaaa tgcaaagcat tagacatgaa ctgctatgga atcaatacat
    54781 ccggtcatcc agcagaagga atggaaaaga ctttttcttt aaatcaacta aaggaagttc
    54841 ttcctgaagt agatttcgta gtcaatatct tacctctaac ggaggaaaca actgggctct
    54901 ttgatcagca tatgtttgaa cactttgatc caaaatctgt attcatcaat gttggccgtg
    54961 gtgcttctgt taagacacaa gatctggtgc aggctttaaa taaccagcaa ctttcctttg
    55021 cagcattaga cgtttttgag gaagagcctc ttccacaaga tcatcctctt tggaaaatgg
    55081 acaatgtact gattacttcc cacattgcag gtttgacacc tgattttcaa aagaaattaa
    55141 tggctatttt tttaaccaat ctgaaaagct actttgccac ccatgagctt caaaccaatc
    55201 aagtaaaatt gtctgcagga tattaatagc tgaaaacaat attttcaaaa gaaaa
//
LOCUS       DACEOM010000009        51671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEOM010000009 DACEOM010000000
VERSION     DACEOM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.010"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IW781_000527"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IW781_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1181547.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3191)
                     /locus_tag="IW781_000528"
     CDS             complement(2886..3191)
                     /locus_tag="IW781_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181548.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3336..4541)
                     /locus_tag="IW781_000529"
     CDS             complement(3336..4541)
                     /locus_tag="IW781_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1181549.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5235..5621
                     /locus_tag="IW781_000530"
     CDS             5235..5621
                     /locus_tag="IW781_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1181550.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5719..5910
                     /locus_tag="IW781_000531"
     CDS             5719..5910
                     /locus_tag="IW781_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1181551.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6424..6756
                     /locus_tag="IW781_000532"
     CDS             6424..6756
                     /locus_tag="IW781_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1181552.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7053..7208
                     /locus_tag="IW781_000533"
     CDS             7053..7208
                     /locus_tag="IW781_000533"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1181553.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7484..8824
                     /locus_tag="IW781_000534"
     CDS             7484..8824
                     /locus_tag="IW781_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1181554.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(9065..9472)
                     /locus_tag="IW781_000535"
     CDS             complement(9065..9472)
                     /locus_tag="IW781_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1181555.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(9661..10488)
                     /locus_tag="IW781_000536"
     CDS             complement(9661..10488)
                     /locus_tag="IW781_000536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAQ1181556.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            10797..11015
                     /locus_tag="IW781_000537"
     CDS             10797..11015
                     /locus_tag="IW781_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1181557.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            11036..11671
                     /locus_tag="IW781_000538"
     CDS             11036..11671
                     /locus_tag="IW781_000538"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1181558.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11864..12103)
                     /locus_tag="IW781_000539"
     CDS             complement(11864..12103)
                     /locus_tag="IW781_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181559.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12127..13650)
                     /locus_tag="IW781_000540"
     CDS             complement(12127..13650)
                     /locus_tag="IW781_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1181560.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(13668..13790)
                     /locus_tag="IW781_000541"
     CDS             complement(13668..13790)
                     /locus_tag="IW781_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1181561.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(13820..14803)
                     /locus_tag="IW781_000542"
     CDS             complement(13820..14803)
                     /locus_tag="IW781_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1181562.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14827..14976)
                     /gene="rpmG"
                     /locus_tag="IW781_000543"
     CDS             complement(14827..14976)
                     /gene="rpmG"
                     /locus_tag="IW781_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1181563.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(14997..15374)
                     /locus_tag="IW781_000544"
     CDS             complement(14997..15374)
                     /locus_tag="IW781_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181564.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15406..15585)
                     /gene="rpmF"
                     /locus_tag="IW781_000545"
     CDS             complement(15406..15585)
                     /gene="rpmF"
                     /locus_tag="IW781_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1181565.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15600..15869)
                     /gene="rpsN"
                     /locus_tag="IW781_000546"
     CDS             complement(15600..15869)
                     /gene="rpsN"
                     /locus_tag="IW781_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1181566.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16392..18164)
                     /locus_tag="IW781_000547"
     CDS             complement(16392..18164)
                     /locus_tag="IW781_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181567.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18118..19872)
                     /locus_tag="IW781_000548"
     CDS             complement(18118..19872)
                     /locus_tag="IW781_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181568.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(19982..20551)
                     /locus_tag="IW781_000549"
     CDS             complement(19982..20551)
                     /locus_tag="IW781_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1181569.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20569..21993)
                     /locus_tag="IW781_000550"
     CDS             complement(20569..21993)
                     /locus_tag="IW781_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1181570.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(21995..22663)
                     /locus_tag="IW781_000551"
     CDS             complement(21995..22663)
                     /locus_tag="IW781_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1181571.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22794..23378)
                     /locus_tag="IW781_000552"
     CDS             complement(22794..23378)
                     /locus_tag="IW781_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1181572.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23709..23942)
                     /locus_tag="IW781_000553"
     CDS             complement(23709..23942)
                     /locus_tag="IW781_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1181573.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(23957..24907)
                     /locus_tag="IW781_000554"
     CDS             complement(23957..24907)
                     /locus_tag="IW781_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1181574.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24904..25746)
                     /locus_tag="IW781_000555"
     CDS             complement(24904..25746)
                     /locus_tag="IW781_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1181575.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(25879..26604)
                     /locus_tag="IW781_000556"
     CDS             complement(25879..26604)
                     /locus_tag="IW781_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181576.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(27065..27379)
                     /locus_tag="IW781_000557"
     CDS             complement(27065..27379)
                     /locus_tag="IW781_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1181577.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(27395..28990)
                     /locus_tag="IW781_000558"
     CDS             complement(27395..28990)
                     /locus_tag="IW781_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAQ1181578.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(29013..29903)
                     /gene="iolE"
                     /locus_tag="IW781_000559"
     CDS             complement(29013..29903)
                     /gene="iolE"
                     /locus_tag="IW781_000559"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAQ1181579.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(29920..30945)
                     /gene="iolG"
                     /locus_tag="IW781_000560"
     CDS             complement(29920..30945)
                     /gene="iolG"
                     /locus_tag="IW781_000560"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAQ1181580.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(30969..31976)
                     /locus_tag="IW781_000561"
     CDS             complement(30969..31976)
                     /locus_tag="IW781_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1181581.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(32089..33249)
                     /locus_tag="IW781_000562"
     CDS             complement(32089..33249)
                     /locus_tag="IW781_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAQ1181582.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(33325..34779)
                     /locus_tag="IW781_000563"
     CDS             complement(33325..34779)
                     /locus_tag="IW781_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAQ1181583.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            complement(34843..35667)
                     /gene="iolB"
                     /locus_tag="IW781_000564"
     CDS             complement(34843..35667)
                     /gene="iolB"
                     /locus_tag="IW781_000564"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAQ1181584.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(35706..37622)
                     /gene="iolD"
                     /locus_tag="IW781_000565"
     CDS             complement(35706..37622)
                     /gene="iolD"
                     /locus_tag="IW781_000565"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAQ1181585.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(37633..38667)
                     /gene="iolC"
                     /locus_tag="IW781_000566"
     CDS             complement(37633..38667)
                     /gene="iolC"
                     /locus_tag="IW781_000566"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAQ1181586.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(38824..39840)
                     /locus_tag="IW781_000567"
     CDS             complement(38824..39840)
                     /locus_tag="IW781_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1181587.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(39942..40139)
                     /locus_tag="IW781_000568"
     CDS             complement(39942..40139)
                     /locus_tag="IW781_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181588.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(40441..41430)
                     /locus_tag="IW781_000569"
     CDS             complement(40441..41430)
                     /locus_tag="IW781_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAQ1181589.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQK"
     gene            complement(42171..43151)
                     /locus_tag="IW781_000570"
     CDS             complement(42171..43151)
                     /locus_tag="IW781_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1181590.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(43164..44042)
                     /locus_tag="IW781_000571"
     CDS             complement(43164..44042)
                     /locus_tag="IW781_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1181591.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(44058..44858)
                     /locus_tag="IW781_000572"
     CDS             complement(44058..44858)
                     /locus_tag="IW781_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAQ1181592.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            46250..46576
                     /locus_tag="IW781_000573"
     CDS             46250..46576
                     /locus_tag="IW781_000573"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1181593.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            46584..47492
                     /locus_tag="IW781_000574"
     CDS             46584..47492
                     /locus_tag="IW781_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1181594.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            48121..48372
                     /locus_tag="IW781_000575"
     CDS             48121..48372
                     /locus_tag="IW781_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ1181595.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            48914..49072
                     /locus_tag="IW781_000576"
     CDS             48914..49072
                     /locus_tag="IW781_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1181596.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            49144..50370
                     /locus_tag="IW781_000577"
     CDS             49144..50370
                     /locus_tag="IW781_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1181597.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(50459..50851)
                     /gene="rpsI"
                     /locus_tag="IW781_000578"
     CDS             complement(50459..50851)
                     /gene="rpsI"
                     /locus_tag="IW781_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1181598.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(50867..51310)
                     /gene="rplM"
                     /locus_tag="IW781_000579"
     CDS             complement(50867..51310)
                     /gene="rplM"
                     /locus_tag="IW781_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1181599.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT        15825 a        10534 c         8205 g        17107 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagta tttgcatcaa cacttgcatt accgaagtcc ttattataga
      181 catctaaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcaataagtt tggtaagatc attatcagta attgaaccta
     1141 caccataatt ttcatgatct ttgcgccgtt caattgctaa atcagccact tttttccctg
     1201 attcaaaaga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagctga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggttcc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta agctcttttc aaacgtaatt accccattag
     1681 gagcagtttg tgtatcagtt gtttcagccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggg
     2101 tgcttggaga aatttcatct tgtgcttgaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaaccaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa tagttatttt cttagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa acttatgtta actagtttac ttaaagaatt tcatgcacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ctctttctta gattaaactt cagagtattc
     2581 aaatagcgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcattcctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt attaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgcttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3301 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattatcc
     3361 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3421 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3481 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3541 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3601 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3661 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3721 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3781 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3841 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3901 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     3961 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4021 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagatttcct
     4081 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4141 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4201 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4261 aaatctttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4321 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4381 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4441 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4501 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4561 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4621 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4681 aagtacattt tatcaccgag aactcatcat ttttcaccat gatgaatact atttacatat
     4741 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4801 aggaattttt cgtttctaaa tactccaatt tgggaatcca ataaaaattt tcgttcagat
     4861 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4921 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     4981 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5041 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5101 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5161 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5221 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5281 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5341 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5401 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5461 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttaata
     5521 aaactgataa agaaattgga aaggttctgg gtattacaag acagggtgcg acaaatttaa
     5581 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5641 tttgtatgct tctttgctcg tcctcctcct tcccaaaaaa ataaatagat tataagttca
     5701 tgggaagaag gtaaatttat gaatacttta aaagaagatt tcattttagc aaagaatgga
     5761 aatgaagaag cagttgtggc cattctcaaa agatttagcg ctttggtcca taagcaatcc
     5821 tggagaactg gaaaatatga tcaagattgt tatcaagaat gtatgcttgc aatatatcta
     5881 gctatctcga aattcgaaat taaagaataa ctcaactaag ggagtctgtg acataagtaa
     5941 aaagatagtt tcaaatttcg aacaataaat ttgaagccat cttctttata attataaagt
     6001 atcgatttta cagcgttcta taatgattca tagaatctaa aacgaatgat tttaattatt
     6061 aacgtttttt aattcgtttt tagcttaaaa ataacttttg tcacagtctc actcctaaaa
     6121 ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc agaactgggc catggtactt
     6181 caacgaggac ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta
     6241 gatttattca actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat
     6301 tttggttgtt aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac
     6361 taagttgggc tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact
     6421 ctattgaaaa aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag
     6481 aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc
     6541 cgatgggttt cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga
     6601 ttacctgata aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa
     6661 gacgagtgtt ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt
     6721 acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt
     6781 tatgcacaag aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag
     6841 aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa ccaaattgat gctgtttggg
     6901 tgacggatat tacttacatt ccatgtaatg acggtcgcct ctatttgtcc acctatattg
     6961 acttagcaac tcgtattcta cgttactata tggtggattc acacatgaaa aaagagattg
     7021 tcgtccgtcc attacaaatt tattaaggag aattgcctac ggtaattcat tccgatagag
     7081 gtagccagca tcggtctttt atttatcaag agctgttgac cgaccaccac atcacacatt
     7141 caatgagcca aactgggact cctgttgaca atgcagtaat tgaagcgttt catcgatcaa
     7201 tcaaatgaga attaatcgac tccaacaaat ataaaagtaa aatagagatg aaggtgttaa
     7261 tccaagatta tttagaagac tactacccaa acaaaagaat ccatacaaaa tttatgatga
     7321 ctccttgaca atttgaagcg aagaaaataa gggatctata atatctagtg ttggtgacgc
     7381 cttctgttgc caatactttt tcttttttct aataaacgca aacagacacc ttgaaatcct
     7441 ttagaataaa gtcgacgaaa accattcaaa ggagtgtttc aagatgtcct attcagagtt
     7501 taccaaagag ttactagata ttctagacct aaatcttacc tttcatgaag atgcctttcg
     7561 caaagagcgc attaatgacg aaacatgttt tgtttttgat ggcacattga cctatcaacc
     7621 agaggaatgc ttccattgcc actatcaaaa taagcaaacg atcattaaat ggggctggaa
     7681 aaaagtatcc atcttattga atgatgtcag taactacaag accattgtgc ggattaacaa
     7741 acagcgcttc aaatgtaaac actgtggaaa gactttttta gcagaagatt cagttagcga
     7801 tcgtcgctgc tccattgccc gcagagtcaa acaagccatt cttgagctat taagtgagcc
     7861 tatttctatg tctttgattg cacgtatgaa gcacatttct ccgaccactg tcattcgaat
     7921 acttcgcagc cttcggccta aaaccgtttc tttgaaccag ccgttaccag aagtggtgtg
     7981 tttcgatgaa ttcaaatcgg tgaaaaacgt ctcgggagcc atgagctttg tcatgatgga
     8041 cggcaagacc catcaactga ttgatattgt ggaaaatcgg caactcaatc ctttacgcga
     8101 ctatttcttg cgctacccta gaaaagtccg tgaacaagta cgtctagtgg tttcagattt
     8161 ctatacaccc tatcgcacat tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga
     8221 tcgctttcat attagccaac acatcggtcg ggcctttacc aaccaccgaa ttcaagtaat
     8281 gaagaccttt aaaaaagggg atcgtcggtc gaagcactta aagaaatact ggagactact
     8341 tcaaaagaat gcgtgggaat tgaatggtca gcatcgctac tggcgtccgt catttcgaga
     8401 tcatctgaca gaagcggaga tagtcgatcg gctattatat tatgatgact ccttaaagcg
     8461 aggctacgaa gtctaccagg tttttctttc cgctatcaga agacaagatg ttcctgaatt
     8521 cgtcgcgctc ctaaaagaag actacaagga gttgccagag cactatcaac cagtgttcac
     8581 gaccttcaag aaatatcgaa cagaaatcaa acgagcgttg cgagttccct attccaacgg
     8641 ccctatagaa tgtttgaaca accatatcaa agtactaaaa cgaattgcct atggctttag
     8701 aaactttcaa aactaccgag aacgaatctt tttatatcga ggaaaatatt tcaagaaaac
     8761 taagaacact acccaactga ctaaagccag aacaacaaca cggctagaca agatagctgt
     8821 ctagccgtgt gagtatctcg attattctat tgaatttctt caccaacact cattgacgaa
     8881 gagccaaaat aagtaatcta accgactgtt aactaaacca ttgcaggtcc cgttaaagat
     8941 acttgtgcct gttgtcaccg cttctttttc ttgtccacaa gcgcttaaca cacttaataa
     9001 agcccctgct attattaata ataatatctt tttcttatta aaaacctcct catctatttc
     9061 cagattagta aatgtagtaa tctttgcttt cttttttctc aatactaata actaacttat
     9121 ggggtaaaca aatactcgtt tgaccgggtt ttgagatcca tccagttcta accgcgattt
     9181 gatcaggact attatcttct ttaactcgaa ttcttccatc tttaatctca acaatattat
     9241 attgtccctt cgcaggatga taattcacga gtttatgatt gatcttatcc aaatcgaagc
     9301 gatccatttc cttgccatca atttgaacga tggcatattt gatagcatct tcagttgttt
     9361 cattctcctt ttctatagag aatacaaata agggaataaa agagggaata atcaaaatgc
     9421 caataattat gacatcgcct atcttgacac cagcttttct taaaaatttc attaacgata
     9481 tctggctaaa catattagaa tttttccatg gaaatggtga aatttttcgg aaattatctg
     9541 cattaagaga atcagttaaa caaccctcaa gttgttcctt aatacattgt tgatccatct
     9601 tcataccaat aaaaactagt ttattaatgc gttcaccaaa caacggatgc caggcttcct
     9661 tcaattcggg tttttttgta taatcatttg acactcaatt ttaggtaaac tagctaccca
     9721 ataatttaat gattcaatac ttttatggct acccgatgtt tcaaagatcg tcgaaaaatc
     9781 actatgttgg gcaaaccaaa gaaacccttt tgctcttgta atattgccag gaaaattttt
     9841 atccgtaaga tgggcaaatt tttgtgcgtc aaggagtaac cgatttcgat agataaaact
     9901 tgagatacca tattcttctg tttcaggaat atgattttga tagccaagct ctaactcttt
     9961 gaaccagccc attgaatagg aagtctcatc tatattaaaa agatttgtat ttagaatttt
    10021 cgataagtct acttcacctt taattgtcct aattattttg gcattggact gtaacttagc
    10081 aattagaccg ttaattttgt aaatcgtctt ggttgacact aagtcactat tatttaaaag
    10141 taatatgtta caacactcga attgatcaat taacaagcca cgaatatcct gttcttcttc
    10201 aactacatta ccttcagctt ttctttcctg aatcttttca cccgtttcga aattatccca
    10261 aaaacgtttt acatccacta ctataataat ggcaccaatc tctaatcgtt gggtcaaatc
    10321 aatgtctaaa gggtttttag ctaatataat tgcttgaata attggaattg gtttgctgat
    10381 tcctgaagct tcaataacaa tttgattcaa gtcagaaaat tgcactaatt gatctagttc
    10441 aacaatcaaa tcttcgcgta atgtacaaca aatgcatcca ttcggcatcg aaacaagttt
    10501 ttctccagtt cttttaaaac ctttcttgtc gactaatacc ccatcaacat ttacttcccc
    10561 tatgtcattt acaatgacag caatttcctt tccgtgatca ccgtacagaa tatgatttag
    10621 aagtatcgtt tttcctgaac caagatagcc agttaagatt gtaataggta ttttttcata
    10681 gggaacctct ttctatacct tgaatttaca ctttaagtgc aacacataaa aatgagtcat
    10741 aatcgatatc caactagtat aagtttgtcg agaacactcc tagaaggaaa ataattatga
    10801 gccagtattg tcatcttact atactcaaac gagaaagaat ctttctgctt cacgaagaag
    10861 gattttctat tagtataatt ggcaacgaca ttcagcatag cccgtttact attttacatg
    10921 aactgagaag aaacaagaaa aaaactaatc accttccaaa gcggaaaaaa attatgctta
    10981 tcgaagaaaa aaatgcggac gttatcacat tctaaatgat ccgtgagttt ggtcaattgt
    11041 tcgtcattta tttgtcgatt ttcagtggtc gccagagaaa atctcgaatc gtttgaagca
    11101 tgaacatatg gacttttcaa ttagttttat cacgatctac cgtgcaattt gcaatgatct
    11161 tttcgataca gaacccctct cacacggcaa taaaggcctg atccgttcat tgcaccatcg
    11221 aggaaaaaca agacatacaa aaaatcatgt cgagtctcgt gggaagattt gccttactca
    11281 ttcgatccat ggccatccaa aatccgcaga tgatagaagt gattttggtc attggaaagc
    11341 cgatattgta gctggcaaaa ctagtggtac ttgtttagtg actttgacag acagaaaatc
    11401 atgcttcctt ttagcaggaa agaaggtccc cagaaagaaa gccgcttatg tgagcgagaa
    11461 aatgattgaa cttttaagca gtattccaaa tgaggcggtc aaaacgatca ctccagatag
    11521 aggaaaagaa ttctccaatc atttcgcagt tacagaagta ttgaataata tgccctttta
    11581 ttttccagat cctcatgccc ttggcaacaa tgaacaaatt aaaatacgaa tagactcatt
    11641 cgagaatact tgccaaaatc gaaagagata gctacggtag ataactcaac gataaatcag
    11701 atgattgaaa aactgaataa cagacctaga aagtgtttag attggagaac accttatgaa
    11761 gtcttctttg ataaagtgtt gcgcttgatt tgacaattca agaaataata aaaagcagat
    11821 caaatccaaa taagggaaaa atgatctact ctggtattat caattaattg gccaagctat
    11881 tatgccgttt tgctaaacgt ctacttcttc cagtattgac cttgataaaa caacaatcag
    11941 gatcccgttt taatactcta tgaactgtgt caatactaac atgtaaagtt gcagcaatat
    12001 cagctaatgt actcccattt aatctcattt gctttgcaaa ttgataacgc cgataagcat
    12061 caatattctt ggaatccata gacaataata cttttcttac catgtatttc ctccaaagat
    12121 tggattttaa tgggccatca tttcttgagc aatctcatgc tcagataatg aagctggata
    12181 gaaagtaggc caattatgca tttcattata aagtttttct tgactttctc caccaaagaa
    12241 aatatggaaa tgctctgcct tacttggtgc aattccatga tcgctaaatt gaatatattt
    12301 aaaacgacca gcacctgcat catcagtttc aaaattaaaa cgaacaccac ggtttccttt
    12361 ttcataattc aaaattttgt atccaacata tttgtatgtg tattgatgat gttcaccgtt
    12421 gactacaaaa tcaatggtgt tgtctttaat gttgatagta tcaatatctg tcttatagcc
    12481 agtagtataa taatctttgt actcggcagc tgtcatgtct ttattgagtt ttgctttgta
    12541 atcgaaaact tcatccaaaa ttccatcttt taacaacgga taaacagatt gccattcgcc
    12601 tgtataatca ctcaattcgc gatccttcac atcggcatct ttaaagtacc cattagcaac
    12661 tgtcttttca tccttcgctt ctttttctgg aactacctct tcaccagcaa ccatagttgt
    12721 tttctctaac gcttttagat tgtcttccat tactgaaaca taatcttcac cattatccat
    12781 ttgttctttt gtaagacttt caagaggatt caatacttcc aattttatcc cagcttcatt
    12841 ggccaaagtt tttgcgattt tatcattagc attcttttca aagtaaatat aattgatctt
    12901 atttttttta acatactctt ttaattcagc taatcgacca gaagatggct cttcttcagg
    12961 acttagtcca gcaataggaa cttgtatcaa tccataatcc aaagctaaat atccaaaggc
    13021 tgcatgttgt gtcacaaaac ttttttgttt cgcttcttta agactagtgg tatagtcagc
    13081 atccaaccgt ttcagtttag tcaaatactt ttcagcattt gtctcaaata cttttgcttt
    13141 tttcggatac aatttaacta actgatcctt gatatttgaa acttccttaa ttgccatttt
    13201 gggtgaaacc cacgtatgag gatctagttc atgatggtga tcttcttctc cgtgatcatg
    13261 atcttcttct tcactacctg gcaataaaat catgtctttt gttccttcta ctacattagg
    13321 cccagctttc ttccaacttt ctttagcttt tggtacccat gattccatgt tttcgttatg
    13381 atatacaaaa acatcagcat catgagcttt tgccatatcc ttagctgagg gctcatagtc
    13441 atgaggttct gatcccgctg gaaccattaa atccacgttc gcttcatcgc cgacgatgtt
    13501 tttggtgaaa tcatacattg gatagaatgt tgttacaatt tctaattttc catctttatc
    13561 attcgatact tttgcatcat tagtgctgga gcaagctccc aataatccta gagctgctat
    13621 cccaataagt cctacactga ttttcttcat tctttttcct tccttaatta tgctaatttc
    13681 ttcattttat tttgtttatg ttctttgata atttttaatg ccctttttct agttttgata
    13741 ttcgggcttt taagtcgtcg atatgcacta gatatatccg ttttctccat tagaaattac
    13801 ccctaatcaa tacttatcat tatttgcttt ccgccagtaa tttatcaaaa ctctgtttga
    13861 tcctttcttc atccaacatt tttccaatta gaataatagt ggtttggtct ttttgtcgag
    13921 tggtgtattg aaaattaacc tgttcgttta ccccttgtat ggcaactata aaatcttgat
    13981 cttgcaaagc aatcaatcct ttaaaacgat acaggctccc ttggttggtt aaaatcagcc
    14041 ggtcaattaa ttggaataaa aaatcttttt ttatgtcttg atctgaagtt aagagaattg
    14101 atttaaactc atgttcttga tgatgttgat gatagtgttt gccattatag tgattttctg
    14161 atgaatcact ttcaaaaact aagggatgat tgaatttttc caaattataa aaatcctttt
    14221 tttgaatcgt ttcactatgt gtgaacattc gaatatcgga taaagggtta atacttttga
    14281 tttctctctg taattttttt agtgcagata aattcaagtt ttgttttcta gacacaaaaa
    14341 tgcgatctgc tatgacaagt tgcttaaggg cttgtggttc tctttcaata agttcaaaac
    14401 tgtcactatc aataacagtc aataaactat cgatatatac tcttcctctt agttgaggcg
    14461 ctgttaaaag tgtttgaatg attggttggg gatctgctat acctgtagtt tcaatgatga
    14521 tttgtgaaat tggttgttga tgttcttcta ttgtttccag taaagaaatc aatattgcta
    14581 ataaatcact gcgaagactg caacacatac atccattatt tagttgtaac acccgttcct
    14641 cgctatgaac taataatttg tgatcaatcc caacttcacc aaactcattt tcaattataa
    14701 taatttcttc cctggggtag ggtgagactt gcaaggcttg attgattagc gttgtttttc
    14761 cagcccctaa aaacccagaa acgattgtaa ctggtatgcc cattttgtca cctttttacc
    14821 acttagctat ttcacttctt tgaaaattgc ccgtctgcgc aattttggcg aatacttttt
    14881 taattctaac cgttcagggt tattccgttt atttttgctg gtgagataca gacgttctcc
    14941 agtttcgaca cattccaaaa taatattttg acgcataaat cttcctcctt caatacttat
    15001 cgatcttcaa gatatgcttg aatttgttgt gcatcttcaa aagttaactc gccagattcc
    15061 aattgtgaaa ctaaattttc tttcatatca acaggtatct cccaataacc atcaatacat
    15121 tgaaataact cctcgtcata ctcttcaaaa attggataga ttcttaaaaa atcatcaaat
    15181 gataagcctt ggtcctcata gtaatctttg actaatccat tttcaattgc ctgttgggtc
    15241 ggtaaacccg tttcagtaat aattcctctc tctaccatcg aacgataaaa agtgcattta
    15301 tctagcgatt gctgggtcgt ttcaagataa tactttaatc cttcttcaac taaaggtggg
    15361 taaataattt ccattttgat tccctcctaa aagttatata ttaaactatt ttgaagctcc
    15421 tttaattact ttctttccat tatagtaccc ttttaaagat acatgatggc tacgtctata
    15481 atcaccattc gcttcgtcaa atgaaatttc tggttttact acagtttgat gagttcttct
    15541 taaccgtttc ttcgccttag atgtttttct tgctggtact gccatgatat ttcctccttt
    15601 taccaacttg ctttttttac tccaggaatc aacccttgat gggcaagttc tctaaactta
    15661 atacgggaca taccgaactt tcgcatatac cctcttggac gaccatccgt ctgatcacga
    15721 agcctcagac gattcggatt tgaatctctt ggcaatttag cgagagcggt gcgatctcct
    15781 gcggctttta attcctgacg tttatctgcg tacttttcaa tcatcgccat ttgtttcttt
    15841 gctttagcaa ttttcgactt ttttgccata tacctttata ctccttttat tttgatttag
    15901 acatctcaaa tcgcaatcgt tacgttttga cactgatact ttaccaacaa gagttgctct
    15961 tgtcaacaat aaataacatg aaataaaact catatgaata gataactatt tgaaaacgaa
    16021 tttatcggta catagagata atcaaatcat ttaaaagaat atgcagttta agctctgaaa
    16081 aataagaatt cacttttatt agtccaatca aacacaaata ccgttttaag tagcttttcc
    16141 tctacttaaa acggcaatat gaaagaaact ataacttttt attcaatctt ttatgtttaa
    16201 ctggtttatc gccaattttt taaaactttg attattcctt atattataag aatagacatt
    16261 aagtgaacta attcaatcat actgattctt tcgtgtattc aaaattagat gctgtttaat
    16321 aaaattattc tttttgaaaa agaattataa ctatatccca aaaattgtag gttaatttaa
    16381 agcctatttc atcatcgtga ttgagcttct ttttcataga cgaatttctc agatagttta
    16441 gttttcttta tgagattttt gtatatttct gaccttcgat acaactcact atgagtacct
    16501 tgatcaacta tttttccttg atccatcact acaattttat ttaccttttc aatagacttc
    16561 aagcgatgcg atataattag tactgtctta tcttgtatta aacgatttaa actttcctga
    16621 atacgttttt cattttccgc atctaaactg gcagtaattt catctaaaat taaaattggc
    16681 gcatttttca aaaaagcccg tgctatggat aggcgttgtc gctctcctcc agaaagtgaa
    16741 cttccgtttt ctccgaccaa tgtatgataa ccgtcaggta gtttttcaat aaagtcttcg
    16801 cagttagcta aacgagctgc ttttttcact tcctcatctg acgcttgact atttccaata
    16861 cgtatatttt ccaaaatact tgtattaaac aaagttacct cttgaaaaac aatagcgatt
    16921 ttgctaaaca aagaagctgg agatacacgt ttaatatcat acccatcaat taaaatacag
    16981 ccttcatcat aatcatacag tctagacact aatttcaaga tactagtttt cccggaacca
    17041 ctagcaccta ctaacgcagt cacttctcct tgtttagccg taaaagagat atgatctaaa
    17101 attggggtat tgttatcata tgagaaagag acatctcgca actcaatgtc aaaagatttc
    17161 agaggactat ctgatccctc ttggatactg gtttccttca tagctctaat tcgctgtatt
    17221 ttaggagcta aataaaatat ttcaagcact gcttctttca tagaatcgaa ggaatccttt
    17281 atttttattg cggctagtaa gtaaccaacg acgtagagta tagtcacttc acccgttaac
    17341 agtaagtgaa cacctactag aatgactact gctaaactaa catatgagaa aatagaggat
    17401 aaggccatga ctgaaaaagt acttaattct acctttaaat gaatcctttc actctcttcc
    17461 atttttttga aaagtctgtc ttgaactttt ttagatagat taaagctatt aatctcttgc
    17521 tgcaattcta tagtttcttg aaattcttcc gagttttctc tcaaagtatc gtaatattta
    17581 gtattggctt tcgtttggga tttctttgat aacaagatta agacaaaact aaataacgtt
    17641 ggcaaaataa cagctaatcc cattttgaca ttaccaacca aaagcatcac tgaaataaaa
    17701 gggaaaaaca gagccatacc gccggcttta ggtattgcat gactcatcgc atgctcaata
    17761 ccttcaacgt cagacatgat agtttgagat aaatctgata aattatgttt tgaaaaatag
    17821 gataatggta aatttgacaa atcgtctgca atttgcattc ttaaatgcgc actttcttta
    17881 taagtactat tatataagtt ttcatactcc ctatctaaca accaatacat gaaaagcaaa
    17941 gtaacgaacg atactgtaaa ataaagccaa ggcggcttta cattttctaa aactaattcg
    18001 tctatcagca tcattataat gaatgctggt accatattaa tgcagtatac aaaaaagcta
    18061 gaaatagatg ctttaaccaa atcttctgcg ccctgattag ttaaagcgaa tttgttttta
    18121 taaaaacttt tcattgtcaa gcctccaatc atttgcattc tcataattag tgaccaattg
    18181 tttatacact tgatgtttag ccattaattc attatgattt ccacgttcta ctattttccc
    18241 ttctgacaag acaagaattt catctacaga tttaatgctg gataatcgat gagctatcat
    18301 aataactgtt ttttctttca tcagatgttg aaaagccttt tgtagttcat actcattatc
    18361 tggatcaata gatgcactgg cttcatccat aataacaatc tttgcatctt ttaaaattgc
    18421 tctggcaata gctatacgtt gtttttctcc tcctgacaga taaactcctt tactaccaat
    18481 taaggtatgc tctctttcgg gaaatttttc taaaatcgat tcgcaaccgg ctaatcttag
    18541 cgcgtcaaat actttacttg ctggcgcttg aggatttgct atagaaacgt tatcgtaaat
    18601 actctgattg aatagtttcg tatcttgaaa gacaaaagca attgtgttaa taattgtgtc
    18661 ttttgaatag tgatttaaag gcttaccacc aattttaatc tgtcccttat ccacattgta
    18721 gaaacctgaa attagtttag ctatcgtact ctttccgctt cctgattttc ctactaatgc
    18781 ataaattctt ttttgttcta aaactaagtt gaaattttct aaaacacact cttctttgta
    18841 tgaaaagctg acattttcaa atgcaatgtc acaattttta atctgtgtct cgttcccata
    18901 ttttagttgg tcttgcttca tttctaaata aagggagtct agtgtttcga ctgcatatct
    18961 tgcttcaaac aagtgcatac ttacatacat tattctcatg aaagaaacaa aaataattcc
    19021 acttaaaaaa aacaacatca acaagtcaac agctaatatc ttgggagtgg taagagaagt
    19081 tataaaaaag gttataggaa ctatcaaaat gcaaactatg ccaaaaaata gccattgaaa
    19141 aagaacaaaa ggtgttttcc cactcaaaga atacttatat gcaaacttag cataatcgtt
    19201 aattgcttta ttcaaggctt taaatgatgt tactttagca ccaaatatct ttataacttg
    19261 aattcctcgg atatattcta ccgtttcaga gcttaacaca tctaacgatt cttgatattt
    19321 ttgaataaaa tttgtattct ctgtcatttt ctttaacaag aatacaccaa ttactaacat
    19381 ggcgattaga accaatccaa ttttaaaatt aatgaaaaag cctaaggcaa ctgataaaac
    19441 aggtgttact atggcttgag cactatctgg aatcaagtga gcaattgcca tatgtgtttt
    19501 agccgcgttg tcatcaattg tctttcgaat atatcccgaa gaatttaaat caaaaaaacg
    19561 aaaacttgct tctgttaaac cgctaattcc cttttttctc aaattagtct ctaagcgaaa
    19621 cccaaggata tgagaaaata agccagaaaa aatataaaat aaagctccta aagttaacca
    19681 agcaacagtt tgaaccgcat aactcttagc taaatattca tctcctacaa taattagaga
    19741 cttaagaaat ttgtaaatac tgtaatatcc atagactgtt aatacgacag aaaaatcaga
    19801 aaataaagtg gctaaccatc cgtagtattt cattttaggg acataactaa ataatttttt
    19861 gtatactttc attttttcct cccccgcatt caatttcttc tcttattcgt ttacaactgt
    19921 taatgctttc cgtaatagcg taaattagag atgtacaaac agatagaagg taaactacgt
    19981 tctatttttt gtattttttt aagacttttc ttccaatcaa tacaccaatc atagataaac
    20041 ttgctacaac taacactgaa atgattcccc atatacctac cgcatagtgg atcattgcat
    20101 ccattgttgc agcagattgt cctttatcta tccaatcttg acgaaaacga tctgtaaaga
    20161 accatatagg aatccactgt ccggcagcac tgcctaattg cattagagcc caagacgcaa
    20221 cttgaggaat tgctcgatca tatccaaata cagatagaat aatatcagct aagatcgcca
    20281 tactaatcga actgataaac caaggtaagt acgccccgcc cattaacgta aaaaataaca
    20341 ataaaacaat tgtgtaaata gtaaaaattc cttttctagg gtagttatag ctaatataaa
    20401 caaatatgat tccagataat aatccaaaaa tccctgggga tatgctgtga ccaaacgccc
    20461 caccaatact actaaccatt attgaaaaaa agtaaattac tagggaaata actgtagcta
    20521 aaccaataaa aatataatct tttgttttca tatatattct cctcaattct atttcgttat
    20581 gtcattaaaa atctgttcta tcaaagttga atcttctact ctaagtttta tatctttttt
    20641 tattccacca ttttttaaat aaataattct attgcatgtt ttataaagaa attccggatc
    20701 atgagtggct ataactactg cacccttttc ttttaagtct ttaataaggt cagcaatcct
    20761 ttccatatta atataatcca acccacttgt aggttcatca aatagaaata aattagtttt
    20821 tgacaatgaa gctatagaaa ccaataatct ttgtttttct ccaccggata aatcaaacgg
    20881 taattgatct ttcattttat aaagttccat tttttttaaa attttttctt gctgatcttt
    20941 actaactatt tcattcccca gggctaactc gctcctcact gattctgtga aaaattggta
    21001 atccatttct tgcattacta aaaacggcga tgacaaattt tttttatttt cgattatctt
    21061 tcctttggta gggcgaataa tcttcatgag tattcttagt aaggttgttt tccccacacc
    21121 gtttttccct accaaaccaa taacatctcc aactttgaca tcaaaattta tgtcttttag
    21181 aatattttca taagtaattt cttcaacttt taatacatct gcacttacct tttcgatata
    21241 gggaacttca atcgaactat taaaaatgtc aaatttcctt aatgcgttat tgttttcagt
    21301 ttttaacaac tgaatagttg tagtttttac agttctattt ttaacaaata gaacttgatc
    21361 aattaattct ttcaaatagt aaaaacgatg ttcagctata acaatagtaa atcctttatt
    21421 ttttaaatca caaagtaagt cagcaagttt cttagtcgct ttgaagtcca aatttgaaga
    21481 cggttcatcc aatataatta atttagagtt tagcgataaa ctacatccaa tagaaatttt
    21541 ctgccgttca ccacttgata gcgaaaaaat gtttttatct aatatatttg aaagatctaa
    21601 taattcagct aattcattga ttcttttatc catatgctct tttgtaaatc caaagttttc
    21661 catcgaaaat actaactcac tcgttgtatt tgttgtaaag aattgtcctc taggatcttg
    21721 acttacgtag ccaatatttt ttgatatact ttcttgtgtt aaggattgta gcgagttatt
    21781 ttcaaataaa atttccccaa ccaattttcc gtcataaagt cttggaataa gtccattcat
    21841 taactttaat agtgtggatt tcccactccc agaagaacca gtaatcaatg taagactacc
    21901 tttttttatt gaaaaattaa ggtcaacaag cgcttgttcc ttaacatcct tatatgaaaa
    21961 agaaacattg ttaaattcaa acaattcttt cacctcaaat caaaatactt aagaaaaaaa
    22021 tagtaacgga aaacaggatg atatagtcta ataagctcat gtttgtttta tagtaagaac
    22081 ttcttttcac aggagattct atcccctttg ttattgcatt agcggtcatt tcttcagcaa
    22141 tcatagttgc acgtataaga acgggaacta acagaaattc caaatattgc agaggctgaa
    22201 aaaaagacaa gcgaatgttt ctcattttta aagattcttt aatcattctt atttcagaac
    22261 gataagtcgg aaagaatctg atcactactg taatacttaa cattattttt ttcggtaaat
    22321 gtattttttc taaagaggat aataattcat tgctagtata gacactaacc aacaaccatg
    22381 agatcattac gaccggcaac attcgaaata caatatataa ggatgtccca aaaaatatag
    22441 taaaaaactc tatcttgata gcatcaataa ttttcattac taaaaacaga actgaataaa
    22501 tcaagatgaa ataaatacat gtctttattt tacgaaacat caatactaaa actgtagcaa
    22561 taatgaaaca ccccacttga aatatatttc cctttcctaa aaaaataatg accggaaaaa
    22621 aaatatttaa agtaatcaag gttaacggat tgatttttga cactctttca cctccaaaaa
    22681 ataagatcgc actgttatct taagctgata aaaatgcacc gttattcaga taacagtgcg
    22741 atcttatcaa aaagcataac tgagaacaat tctcaatgca agcttttttt taattaactc
    22801 agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa tagcatagtc
    22861 ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa tgagtaaatg
    22921 tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc gatagtcttt
    22981 aacttatcta gacaacagac tgtctttttt gtcacaaatg atgtaatttt ttcaacaata
    23041 tcttgatatt tggttccagc tgattggaat aaaaccaaat cgaatatacg tttattctca
    23101 aaaatataat taataaatgg atctaattca ttgaagttct ctctccaact tttctctata
    23161 tctatatttt ctaagtttaa gaggtatttg gtaaactctt cattatacat attaataagc
    23221 gcattaatat gcggctgaat aatttcttca aataacgctt ccttagaagt aaaatgttta
    23281 taaaaagcac ccgtagttac gcctgcggtc aagcacagtt gccttatctt agtttgctga
    23341 aatcctactt tttcaaaatg gcttagtcca ctttctatta atttttggtg agttgtaata
    23401 taatctctgg tttctatggg tacttcctcc taataagatg aattaactat attatcttac
    23461 acaggacttg aagtttcaat cgttaacgtc tattactgaa ctcattcctt agagctaaac
    23521 taatgagaaa gagcaaaata actgatacaa aaagcaatcc tacaagaatt aatagactca
    23581 tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat tatatccagc
    23641 tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct ccctaaaaat
    23701 aaagcctttt acttaaaacc ttttttgtaa tcgtcaataa attttttccc tttatcagtt
    23761 aattgaaatc ctatatctaa atagtcctca tatctaagtt tagtcagcat ctcacttaca
    23821 gatgctggac taacacttag aacttttgct aaatcattat tttttaattt cttttttctt
    23881 tttgataatt cctgaattgc tagtaaataa attagtatag tagttgtttt aatttgtacc
    23941 atacctaccc tccaaattat ttcgaaaggc cttcagcaat tttatccagg ttccatttca
    24001 tcatcgcata gtaactatca ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg
    24061 ttgaatagat aggaatattg gtatcttttg atactgtttt cattggtcta tcgtccacac
    24121 tgctttctac gaataaggag ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta
    24181 tttgatctgg tgttccttct tcttcagtgt tgatttccca aatgtaagca gaaggcacat
    24241 tatacgcttt cgagaaatat ttaaagcatc cttcacttgt aacaatcatt tttttatcat
    24301 tcggaattga agcaaattta gatttagctt ctttgtctag agaatccaat ttttcaatat
    24361 acttatctag attttcttta tagaattttt tattatcagg atctttttcc gctaactgtt
    24421 tttcaatatt tttagcgtaa ataataccgt tttctaaatt taaccaagca tggggatctt
    24481 ccttcccttt ctcactctga ccctctaagt aaataacatc tatgccatca cttgctgcaa
    24541 aatagtcttt gttttcctct ttgttcgcat tttttactaa ttttgtaaac caagcatttc
    24601 ctccagtttc caagttaaca ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa
    24661 catcttcagg caaaggttca tattcgtggg gatctttccc aataggtacg atactgtgta
    24721 aatcaatttt atcttttgct atattttcag taatatctgc taaaatcgaa ttagtaacta
    24781 ctacctttaa tttttcgttc gaagccactg tatctttgtc tttactattt gttgaacatg
    24841 ccccgaatat aaaaagagca atcaaaatcc ccagacttcc cagtactgct ttccattttc
    24901 tcatcataac tttctctcct tctttcttaa aaatttttgt tttggtgaga ttagaaaact
    24961 aattacaaat aagaatgccg ctgttaaaac aatacttgat cctgctgcta tgttaaaact
    25021 ataaccaatg aacaaaccta gcaatgatga gcacgctccg actgtcgccg aaataaatat
    25081 catagttttt aaactgtttg catataaata tgctgtagca gctggtgtaa ttaacattgc
    25141 aacaattaat atagttccca cactttgcat agctgttact gctactaaag ttaatagaat
    25201 cattaataga taatgataaa aactgacatt catccccatt gcttttgcca ttaatggatc
    25261 aaaagaagtg attaataatt ctttaaaaaa tagaacaact gtaaccaaca ctaaaacaga
    25321 aacgccaatt gtaatccact tatcaatatc ttgtacagct aaaatatttc caaataagat
    25381 atgaaataaa tccgttgagc tatttgcaac tcctataagg ataactccta atgctaaaaa
    25441 agaactgaaa gtaataccaa ttgctgtatc tccttttatt acactattgc tttttataaa
    25501 agtaataatg atagaagcta atagaccaaa tacaatcgca ccaataaaaa aattaatacc
    25561 taaaatataa gaaattgcta ctcctggcaa aacagcatga gaaatagcat cacccataag
    25621 agacattcct cttaaaataa taaaacaacc tactgttcca gaaacaatcc ctataactac
    25681 agatgtaatt agagcattct gtaaaaagtg aaagtcatac agaccattaa taaaattttg
    25741 aagcatatta ttccccttgt atattagagt tagggtttta accttccggt tttgattgga
    25801 taaccctatg aaccatcgtt attttaattt ggtattaact catttctttt gagataatat
    25861 atttattgga ttgagaggct aagccgttcc cccctctcca ataaatatag tatctccata
    25921 ggcttttttt aaattctctt cattaaatac agattctaca cttccgtgag caattagatt
    25981 tcgattcaaa agaataatat tatcaaaata cttctttacc ttgcttaaat catgatgcac
    26041 aattagcact gtttttccct gatgtttcaa ttcttttaac gtatccataa ttattcgttc
    26101 actaactaag tctatcccta caaatggttc atctaaaaat atgaaatccg cattttgagc
    26161 aagacatctt gctaaaagta cacgttgaaa ttgtcctcca gataattctc ctatttgatg
    26221 attagaatat tttcccatat cgaccttttc caaagccttt gaaactcttt gccattctgc
    26281 atttttaatt ctttgaaaaa ctttcattcc ggcatatgtt cccatagata cacattcttt
    26341 tacttttatt ggaaaagtaa aatcaatatc agctttttgc tctacatatg caatcttcac
    26401 cagtttttta ttgatatctt cattattaat ggagacctct ccctgatgag ggataatatt
    26461 taacattgct ttaagcaatg tagattttcc agcaccattt ggaccaataa tacctgttaa
    26521 agcaccttct tgtattttta aagaaatctc gttcaatgct aaaaaatcgt tataacaaac
    26581 attaagcttt ttcacttcta acataacttt ctccttctaa atgataatga ttatcacgat
    26641 catatggtat acaaaaatta tattttagtc aaccctaaat tttataattt agattttttt
    26701 ctgttgttga agattaggat gtcaaaatta tttgaaacta ctcgtatagt aaatagtaag
    26761 taattttcaa aacacgaaga aaaacaaaaa gaaaaaagga aacgccgaag catttccttt
    26821 cagtgattta atgagaacaa cacgatttga cttagagtct caaaaagtcg aataggtaca
    26881 ctcatttaag tgtctccatt cgactttttg gggatgagct ggctattgag gtccgcttac
    26941 cctattgaat aaatacaaga ttgggtattt ctaataagtc cgcccttcat attggggtga
    27001 tcggggcgaa catgccatct tttcatatac cacctcctcg acactcaata atcaaagatt
    27061 ctcgttaatc ttcttcgatg cttgcaatga tgttttcaat attcatataa cgaaaatctt
    27121 cttgtttgat ataaacgatc tgagcggtac atttttcctt tttgatgatt tcttttactt
    27181 tttcctcaga acgtgcgacc tgcggcgtta aaagtaaaac gtcatagtct ttataattca
    27241 gtttttcttc aacgtttcgg atcgttgcat aatctatctc atacgataga tttttggctc
    27301 tgatcgtttc aagcattttt gatacgagca gcgacgcact gattccggtc tcgcaaataa
    27361 tcaatagatc cttatgcata ataccctcct gtttttagcc tgcaaagcca attggtgaaa
    27421 atgccacata agttaaaata acaatgacta ccaatgctat agcagtcacc ttggtgtatt
    27481 tccaaggctc gatatcgacc tttgtatcga atgagttcaa cacaaattct ccatcaggtt
    27541 tcaatttgga aaccaccatc aaaatgataa tgtccagaac gaataatgcc gtcaaaacat
    27601 aaaggaaatg gatattttgg aaaagcattt ttgatagtgc atacaagacg atatgcacgg
    27661 caatcgtcgt ttttgcccca aaagcagttg ctttttttga ataaaaggca aagagaatga
    27721 tcacgagcag cggcatgttg tatagtccat tgaattcttg aacgaactga tacaatccag
    27781 ccggtgcaaa agaaatgaat ggcgctacga taatggagat aatccccaca acgaccgtta
    27841 ctgttttacc agcacgagcg atttgcttat catttgcttt cttattgata agcggtttat
    27901 aaaaatccag tgaaaacaag gtcgatgtcg cgttcaaagc tcccgcaaat gatgataaga
    27961 tcgcgccaaa gataacagcg gcgaaaatac caaagatcat tttaggcaat acttcactga
    28021 ctaaaaaagg atacgcgtta tctggatttg acattaaact atcgccaaaa atattgcgag
    28081 cgacgatccc tggaaagaca aggaataacg cacctaagat tttgaagacg ccgactagga
    28141 aagcgccctt ttgtgcctca gctaaattct tgccggacaa tgctttttgg acgatcattt
    28201 gattggtaca ccagaaatac agattgttaa acagcatccc tgtaaatagt gtcggccatg
    28261 gaacaacagc cgagtcgatc gatcccacag agttaagcag ccatggcgtg tttaccacaa
    28321 cgtgttcgat cccgccaaca aagctgccgt caccaagctt cattaagcct aaaattggga
    28381 ccagcagacc acaagccagc agaccgactc cataaatgga gtcactattg gcacttaacg
    28441 acaagccgcc gatcagcaga tagaggatgc cgacaatacc aattgccatc gctacaatta
    28501 tgatcgcgac gatcggctgg acgcccagca gctcatcaat atggaagatc ttattgaaca
    28561 ccagcgatcc agaataaaga accacgggta agaaagaagt catataggtc acaataaata
    28621 agatcgaaat aattcgtttg gtaaccgtat cataacgaat ctcgatgaaa tctgagattg
    28681 tattgatgcc gtatttaaaa tattttggca agaatatcac agccaaagct acgatcgcaa
    28741 tcgtagcagt tacttcccag gccattactt ccatccctgc atgataactt tgaccatttt
    28801 gtccgactat ttgttcggtg gacaggttcg tcatgatgat cgtgccggcg attggcaaag
    28861 cagttaagct ccgccctccc atgaagaacc cttctgatcc agaagtatcg acacttctgg
    28921 atctagaata ggcaaataac catacacaga tgacaatcac taggaaactg attatagaaa
    28981 aaagattcat cgtctagctc cttttcaata gattaaagac cggtgacctc tcgaatgtag
    29041 cttctggctt tgactgcata ttcaaatgga ttggctttag ccgggtcctg ttctgcttca
    29101 actacgatcc agccttcata gtcgctggca tggattgctg tccagattgg agtgaaatca
    29161 atcatcccat caccaggaac cgtgaagacg cctttcttca cgccgtttaa gaagctgtct
    29221 ttttcctttt ctacttcttc agccgtttct tgacgaatat ctttgaaatg gatgtgtttg
    29281 atacgatctt gatgacggtt gtaaaccgcg attggatttt caccagaaaa gaccaaatgt
    29341 cccgtatcga acaataattt aactttgtct ggatcagtca tttccattaa acgatcgatt
    29401 tcttccgtcg tttgaacgcc ggtacccatg tgatgatggt aaacgatctc catatccttt
    29461 tcgtgggcca aatcccctaa tttttctaat ccttccgcca gttttttcca ctcttcatca
    29521 gtgaaaactg gtttatcatc aaagactggc gctgtcataa tcccttggat actatgaccc
    29581 tgctcagcta ctacgatgac tttagcgccc atcgcatgta agaaatcccg atgtgctttg
    29641 aaggcttcgg cagtttcttc aaaaggttta gttgtcagaa acgcactgaa ccatgcactg
    29701 gcaaccttta agccgcgaat attcaaatat tgttccaaca cctctggatc ttttggatat
    29761 ttgttgccaa tctctgttcc ggtgaaccca gcaagggcca tttcactgat acattgttca
    29821 aaggtatttt cactaccaag ctcgggcata tcatcattgg tccacgcgat tggtgcgatg
    29881 gctaattgaa tttgtttggt catattctca cactcctgct tattttttta gttgtactaa
    29941 actcttcgta tcgaatgatt cttgacaagc caacgcgatc ttagttgatt gcaatccatc
    30001 ttctgcatta accgctggtt gacgtttttc ttgaatacat gagacaaatt cttgggcttc
    30061 acgaatgaag gcttctctga atcgttctgg gaagttttgt gaacatggac ggatgatccc
    30121 ttgatcgttc atgatcgtca caagattttt ctcaggcgct tgcgctaccc gcagcattcc
    30181 tttggttccc atgatctctg tttcgacatg atagccatgg gctgcgtttc tgccggcaac
    30241 tagtaaagct attgttttat cgctcaggct catcattgcg gctccagttt caagctcacc
    30301 agcctgatcc aattctggat acgccgcatt tttcccaatc gcccacactt gatcgacttc
    30361 ttggccggta aaccagcgaa ccaaatcaat atcatggatc gccatatcta agaacaagcc
    30421 gccgctgtcg ttattcatgg caaatttgac aaagctttcc aagccttcgc tcggatcgat
    30481 cccataacaa cgaatcagtg tgatttcgcc gatttcgcca tcttctacca ttttcttcgc
    30541 atactgatac gactcatcat agcggcgcat gaagcctaac atgaagacct gttcaggatg
    30601 ctcttcgatg actttttggg tctttttaat atcttcaact tcaaggccta atggtttttc
    30661 agtaaagaca tgcttgccat tttccatcgc caagcggact tggtcacagt gaaagcctga
    30721 tggtgaaaca ataaagactg catcgatcac ggaacttttg accatttctt catagctgga
    30781 aaaagtatgc ttgacgccta atttcttctg tgcgaatgct aattctgttt caactaaact
    30841 gcacgcagcc gtaagctcac aacaactgac acctgttgct aaattttgtg catgtacttt
    30901 tcctaatctt cctaaaccga cgatgcctac tctaatgttg ttcatgaaat acctccgttt
    30961 tctgagaatt actcattgta tagtgctggt cgttcatcca tttgaatcat tacttttgtt
    31021 tttgtttcac gagactcatg acaagccatc atggtgatcg ctgctacata cccatcccaa
    31081 gcggtaggac cgacaaccgc atcatttttg atcgaatcga tccacagctt gaattcataa
    31141 tcataggcat ccaaaaagcg ggtctgccaa tctggtgaaa caggtgtgta atttttttct
    31201 tccgatttaa ttcgtgtata ggctgggtcc gtcattccta cttctgccgt ttcgaaaacc
    31261 acatcacatt taatatcgta gccaaagcgg caattcacaa agatttccaa atcaatcgtt
    31321 acaccggatt ttgtttgaaa gatcagcagc tgcggatcat gcaaatcggc atgggagttg
    31381 ctggtttttt tcggcaagat catttgaaca gattcataat cttcatttag caaccagcga
    31441 agcacatcaa tctcatgaat cgcggtgttg ctgatcgcca tcggtgttgt gtagttttca
    31501 tctgcgctag gatttctatg ggcacaatgc atcattaatg cttctccgta ttgcttcgat
    31561 tcgattgctt ctttcagttc aatgtagccg cggtcatagc ggcgcatgaa gccaacctga
    31621 accaatttct tcccataagc aacttccgca tcaacgatcc ggcggcagcc tgctgcatcc
    31681 atcgctaatg gcttttcgca gaaaacatat ttcccatgct tgattgcttc taacacatac
    31741 tgctcatggg tcgggtccca agaagtcaca acaacgacat ccacatcttc agaagcgatc
    31801 aatgcttctc cggtttcaaa gaatttagca tcgacacgtt tggcaacctc tttagcagct
    31861 tttacattga tatctgagac tgctgtcaca taagcccctt gtgttttctg attgatcctg
    31921 tcgatatgct cgatgccgat tgcacctgtt ccgataacgc ctacttttac tctcaattac
    31981 ttatccccct gttctgttgt ttaagcacac atagtatgcg cttacaaaat tattcctgtg
    32041 tagcttttat tcttaaaagg tagagccctc tctttgctcc gccttttact acccataagc
    32101 ttgtacgtat aattccttaa tttgactatg actaggcacc tttttattat tttgcggact
    32161 tccgctggtc aacgcatccg cggccatttt gtctaaggct gccataaacg cttgctgatc
    32221 aataccataa tctcgaatcg ttgggatcga cagctgctca cagagctctt ggattttttt
    32281 aatcagataa tccgcagcct cttccagcga taaatcttcg gctgccggat aaataaatct
    32341 ggcgatcgtc gctaactcag tctgaatagc atcctttgag aattcaaaaa agaccggcaa
    32401 taacatcgca tttgatatcc catgaggaat gtgaaaaagt gcccccaccg gtcgactcat
    32461 tccatgaatc aatgtgactg acgcattcga aaaggcaatc cccgcctccg ttgaagctaa
    32521 gctcatttgt tctctggctt cagtgtcgtc gctattttca tatgcctgag aaagcgtgtt
    32581 caaaattttc ttcatcgctt ccagtgaaaa tagttgcgtt acttcattgg ctttgactga
    32641 aacataagat tccaaagcgt gacatagcgc atcgactccc gttgccgcgg ttacttttgg
    32701 tggtacagtt ttcgtaaaat cagagtcaac gattgagatc tgtggcatga aaacgagatc
    32761 cttcatcatc attttgactg ctgtttttgt attggtaata actgccacat cggttacttc
    32821 cgaaccagtt ccagcagttg ttgacactgc aatcagcgga atgcccgctt gcagctgatc
    32881 aaaatttctt gcatgaatcg cttcgagact taattgatta gcagccaaaa cggctaccac
    32941 cttggctgta tccagacagc tgccgccacc aaaagcaatg atatgatcac attgatgctt
    33001 ctgaaaaagc ttcagcgcat cataaacatt ttcttgcgtc ggttcggctt gaatgtctgt
    33061 atactccgct aattgatcat ctgtttttcc caacagctcc ttgatctttt gaagatgccc
    33121 ggtctgttcc ataaccgtat cactaatgat taacggcttg ttgcctagtt gttctgattc
    33181 ttccataaga aattggattg ttccgccgcc aattcttaaa ttggcaggca ttttcagttg
    33241 aatattcaca gaaaaaacct ctctctggct tatagcctat aacagaatcc tcgaagctct
    33301 ttaacagagc ttcgaagcaa atctttaaat ctcactttgc gcataacgtg ctgtaacaac
    33361 tttccgatgg gtataaaact caacactgtc tttgccattg gcatgcaaat caccaaagaa
    33421 agagtgcttc cagcctgaaa aggcaaacca agccatcggt gcaggcaccc caagattgac
    33481 ccccagcatc ccagcatcaa cattttctct gaagtagcga atcgctgatg cgttgttggt
    33541 atacagacac gaaccattcg caaattcaga ttcattcgtc agctcaacgg ctgccatcaa
    33601 atcttttacc cgcacgattg atgcgaccgg ggcaaaaatc tcatcctgcc agatcttcat
    33661 atccatcgtg acattatcaa aaatcgttgg tccaacaaaa tagccttttt ctggaatgtt
    33721 ttcgcgacca tctagtacca gtttcgctcc ttcatcgacc cctttttgga tatagtcaaa
    33781 ggtccgctgc tgtgcatctt ttcgaatcac tggtcctaaa aagacccctt cgtccaagcc
    33841 attgccgatc ttgatcgctt ttgccgcagc cttaaatttt tcaacaaact gatccgcgat
    33901 ttcctcttcc aaaacaatga cggaggctgc catgcagcgt tcaccagcag aaccaaaagc
    33961 tccgccaata aggtccttga ctgcccgatc aatattggca tctttcagca caacaacatg
    34021 atttttcgct ccagtcaacg cttgtacccg ttttagattt tttgttcctt cacggtaaac
    34081 gtattcaccg acattttttg atcctacaaa gctgatgcct tttaccagtg gatcttgcag
    34141 caatgtgttg acaacatcgt gggcaccata gacaacatta aaaacaccgc taggaaagcc
    34201 tgctttttca accagctcaa caattttctc catcaacaat ggtgtttttt ctgaaggctt
    34261 cagcaccacc gtatttcctg ccgccagagc catcgggaag atccagaagg gcaccatcat
    34321 tggaaaatta aacggtgtga tccctccgat tactccgatt gggtaacgat aattcgtgat
    34381 ctcaatatca gtcgccgctg ttgataaaga atctcccatc attaaattgg tgattcccgc
    34441 tgcgtgctcc acattttcga ttcctcgttg aacttctccc agtgactcgg acaatgattt
    34501 cccattttcc agcgtgatca aacgcgccaa ttcactttta tgatctgtta aaagctgatg
    34561 cagcttgaac agataacgtc cgcgaacagg aacaggaact tttttccaag tttgaaacgc
    34621 agcatcggca gctttaactg cttcggctgt ctctgctttg ctagacaacg gtacattcgc
    34681 cagcgcttct tctgtagccg gattcattac ttcttcaaac tctgttgagc gactagcgac
    34741 ccaacgacca tcaatataat ttcttaaaaa tcttgtcatt tattttcctc ctaaaaacct
    34801 aactaaacct aactaaactt tatgtatcta acagcttaat aactacttgc gctgcaagat
    34861 ccattcgtgt ttcttttcat tgtggaattt ccaaattcgc tcaggtcccg ccatcacatt
    34921 taaatagtag gaagtatacc cgtcaggtac cccaactgga tgatagccct taggaacttc
    34981 gacgacattt tgatgctcta ccgtcatcgt ttcatctagt gaacgatctt ccgtgtagac
    35041 ccgctggaaa acaaatccct gctctggatc aagctcatgg taataagttt cctctaaaaa
    35101 agactcatgt ggcagattct cttgatcgtg cttatgagga ggatagctgg aaaaattacc
    35161 gccctcggta taaacttcga cgaccagcag gctgctggca gtatcgacat tatccggtaa
    35221 aatgttgtga acaagtcgtt tattctgata gagtccacgt tcttcgacgg tattatcctc
    35281 cgcgcgaatc acacttatcg gcaaagcttt tttcgctggt gaatagcaaa gcgccaatcg
    35341 gacattgtct gtagttgcca caagttcaaa cgaatgctcc gccggaagat aaatactgtc
    35401 ggttggaatt ttttcaaaaa cagattctcg tgttcctaaa tcttcatacc aagtgtctgc
    35461 tgctttaacc ttaatttttc cagtcaaaat gacgatacag acctcctgtt gactgccggt
    35521 ttccttgtaa gaagctccct tttccagctc caaaatctga aattcgatat aactcaaagc
    35581 agaattagac tgccgaatat tttggatttg ttttactcca ggtgccagtt ctttgtttaa
    35641 tggttgatgc ttcaatgtct tttccaatca atccactcct tattttcaat cacaaaaagc
    35701 aatatttaat actttctgat agtggcaagt ttcgcttgtt tggctttgta agcctcctgc
    35761 atggctgctt tacccgatac ctctgagata ccgacgttcc accagctgcc gtcgtagcct
    35821 tctgtcatgg ttttaggaag tacctttatc tcaatcaacg tcgatcgcgt ttgcttcttc
    35881 gcatcctcga ccgctgcttg taattctgca atcgagctta cacgatacgt ttttgcacca
    35941 tagccttcag caactttggc gtaatcgatg ttcatgatct cgtccttggt atttctgaat
    36001 tcggttcctt ggctagctcc gccattatcc atctgtaaat tattgataca gccgaaacca
    36061 gaattatcga aaagtacaat gttgattttt ttatcatatt gcaaactaga aaccagctcg
    36121 ctatgcaaca tcaagaaact gccatcgcct actaaggcat agacctcttg ttctggtcga
    36181 gccattctta cgcccatcgc accagcgatt tcatagccca tacaagaata accgtattcc
    36241 aaatgatagg tattctcctt cacagggttc cataagcgct gcatatcccc cggcaatgaa
    36301 ccggctgaac cgatcacgat cgcatcctca tcaacaaaat catttaaatg aatgaagacc
    36361 tctgtttgcg tcaaattcgt agccaacgca tcagcatatt cattcaagat ttcttgagaa
    36421 aaatgatctt tgatttctgg cgtaaaatct ttacgcttaa aatgcgcggc ttttaaccga
    36481 tttcgctcac tgatccattc ttccttcagt tcatcgatct tctcgccata ggcggtttgg
    36541 taaccttcga tcattggggc aagctgctcc aaggtcgctt tcgcatcacc aaccacttga
    36601 aaggcttcca gcttagcggc ctgttgtctg cttacgttaa tattcatcat tttcgcttgt
    36661 tcaaaattaa aaatactttt agaagctgag gtaaaatcag tgtagcgtgt gccgatccct
    36721 atgatcagat ccgcttgctg cacagcttta ttagcagccg aagttcccaa aatccccatt
    36781 ccgccgagat tgtttttaaa atcccacgaa acagtcgatt tacctgcgtg cgtttcaact
    36841 agcggaatct gacaatctct tgagatttgc tcaagaatct caccagcctc cgaatatttg
    36901 cagccgcctc ctacgatgat gacaggtcgt ttactggcct ttattcgttg actcgcttca
    36961 gcaagctcac gagcggtcgg aatcctgcga tcaatataat gtactctttt tttgaaaaat
    37021 tcctccggat aggcataggc ttctgcctcc acgtcctgcg gcaaacaaat cgttgccgga
    37081 cccatcgttg ccgggttcgt catgacttca aaggcccgca ataacgcgga catcaactgc
    37141 tctggtcgat tgatgcggtc ccagtagcga gaaaccgctt gaaatgcatc atttgtcgaa
    37201 aaggctacac tgcttgcatg ttccagctgc tgcaagaccg gatcaggctg acgtgtagca
    37261 aacgtatccg ccggtaaaaa aagtacgggg atattatttg cgaatgccgt acccgctgct
    37321 gttaacagat tggccgcgcc cggtccaaca gatgttgata cggcaaaaat tttcttgcgt
    37381 aaattctgtc tggcaaaggc agtagccgca tgggccattc cctgctcatt ttttccgcta
    37441 taacttttca aatgtccggg ctctaattcc agcgcctgac cgatccccaa aacatttccg
    37501 tgaccataga catgaaagat accttcaaca taaggcgttt ctttgccgtc gatgctgatg
    37561 tattgttgat tcaaaaattt tagtaatgct tgtgctgttg ttaaacaaac cgtcttttcc
    37621 atttttttca ccttaaatcc gagcgatcat ttcgccgtat atttctttct ccaatgtaat
    37681 aaaatctttt acctcctgag tcgtcggtaa agaatctgaa cagttattgc ttttgaccat
    37741 cattgatgct tccgctgagc caaactctaa acaatcgatg atctcccagc cttcgaataa
    37801 accgtataag aaacccgagc cgtaaccatc tccgccgcca aagcctttgc gtgcttccac
    37861 cggaaacggc ttgattgaaa actgctgctg atcggccgta taagcagtcg atcctttcat
    37921 tccatgtttg attacgacaa tctcagcacc acaactgtgc caaaaagcag cgctttcatt
    37981 atctgacatg ttaggctgga tcaacttctc agttagatcg aactcttctc ttgaacccat
    38041 gataatgtct gattccttag caatcatcga atagtaaatt gaaatatcat cgttgttctt
    38101 ccagttataa gcacggtaat caatatcaaa aataattttg acattattcc ttttcgccag
    38161 catcactgcc ttgattacgg cttctctgga cgggctctct gctagtgcag ttcctgagat
    38221 taatagtgct tttgaccgtt tcaagtagtc ctcatcaata tcctcaacgc taagctgcag
    38281 gtctgcggct tgatttcgat acatcaaaat attactttcc tttgatgaga gcatttcggt
    38341 aaaagtcagc cccagctttt caccatgctg cgctttgctg atctgagacg tatcgatgcc
    38401 ttgttcattg aaataatcga tcacatattc gccaaactga tcgtccgaga ctgttccgat
    38461 aaaaccagct ttcatgccat gtttagtaac acccacagca atattggcag gagatccgcc
    38521 cacaaatttt tcaaacatat ggaccttttt caatggttta aactcttctt tgacttgatc
    38581 attgtaggca ggattaaaat caatagcgac tcttcctaaa agcagcaagt caaattcttt
    38641 tgattggtca aactttatat aactcaatat atccacctct ttatccgttc ttctatttct
    38701 gaggcaagta tagcacgcca aaacaaagcg tgcaatattt attttagaac gcaatattgc
    38761 gtgctgtttg aaaatgacgg caaaaaaaac gtgaatcgcc ttacatcacg attcacgcct
    38821 agattaaata ttgtattttg tcttaataat tatatcagta aatagcgcat cttttgtaac
    38881 cgttttatta ttttgcagct gattcgctaa catcagtaat gaacgatagc cttgaacaaa
    38941 tccatcttga tcgattagaa aatcaacctg atcgtttttc agcatctcga tattgttctt
    39001 agtcaaatca taaatgatta caaagggccg cttgctttca tcgatttttt ccaacgctcg
    39061 ggcaattccc gcttgtccgc cagaaaagac gactacgcca tctaagtttg aataattctc
    39121 caaggtgttg cttaaaatct tttcaacctc agcactcgaa tcaaagctgc tctgaacacc
    39181 cgtcagctcc agttcaggaa acgactcttt caattcttca acaaagcctg ccacccgtaa
    39241 gctgttgacg ctatttccaa agtagccggt aatcgctaga accttgccct ttccttttgt
    39301 cagcatacct aacaatcccg cagccgtttt tccgcttttg aagttgtcca tcccaacaaa
    39361 acactgtcga ttcgtaccaa gaatatccga attaaaggta atgatgagga tgccttgatc
    39421 ggtcaattga ttaatttttt ctctgatacc atcactatct actggcatga tcgcgatccc
    39481 ttttactcca agctccacca atcgatccaa cgcctgcagc tgggccgctt catcgacccc
    39541 gtctacctct tctaaaaaac aatcaatgtt tcgctttttt aaatccccga taatagattc
    39601 cagacctgat cggatcggaa tcataaaaga agatttcgcc aattgagtga caatccccag
    39661 cttgagcgtt gtctctttta tgaccttctt tttctttgga acatagttca tttccttggc
    39721 gattgctctg attctttctg caacctcagg attgatcctt cctcgatcgt tcaatgcccg
    39781 atccactgtc ccccttgaca ctcctgcttt ctcagctatt tctttaattg tacccgccat
    39841 cttgcctctc cttttttcct attgtgttta ttttatcaca aaacttaaga aaaccaccgg
    39901 agaatgaatt ctccggtgga ctttttgtat gattaacctt cttattgaac atcactgatc
    39961 ttaaactcta ttccaagggc gtttctataa tattctggat acatctcctt cccacatttt
    40021 tcacaggtaa attggggagg attgttcgga tcatttgcaa tatctgcatc atctaaaaat
    40081 tttactacat ccattgggat tgattctttt tcaccacaac ttagacatat aaatttcacg
    40141 ttggcgttcc caatcccagc gaattgcttt aaaagttttt tctttttctt cttcatactg
    40201 ttgcctgaat tctttttggt caatttttcg attctcctct cgcataaacc gctttgcatg
    40261 gttatctttc gcatatttaa taactaaccg tccatttttg cgcacactca tcccaacgaa
    40321 ttcatagtac tcaccgcaac aagaacactc taagggattt tcaccaaatt cttcggttat
    40381 tctttcttcc caggattttg gtcttaaggc ctcattcaga ttgataaaca gcttttttac
    40441 tcatttctga tactccttca tgaccttttt cattaatgtt ttgattctac gactatagat
    40501 tccatagcga cgaatcgttt tgaaattctt attttgaata tggcgaatca gtgcagctat
    40561 gaattcgtct atactcatgg tcatcgtctc tttagtattc gttcgtttgt ccatgtagtt
    40621 gaacatgaca gtgtcgccat catacattaa gattcggctt aatgcgatcg ggccacgctt
    40681 catataacga ctgatgtatt ggagtagtga ctttagattt gtcctgctcc ttttaggagc
    40741 atttacgtaa aacccttctc cattgttctt gtacgcagct tgtaatcttg attgaactct
    40801 ttgcttgtcc cactcaccta acgttcgtct aattagtttc aatacagcat ttttccagta
    40861 ttttcttaac atagaaaaag gtaaataatc gtattcttcc caacttccat ctcttgttaa
    40921 gccacccata gtaacaacca tatgaacatg cggattaaat tctagttttg atccaaaggt
    40981 gtggattgta gctactatac ctgccttcac ctttcttttc tcaaaatagg caagaataat
    41041 ttttgcggct tcatccatta gtcctttcag taaggaatct cgatagtttt ccatcataaa
    41101 gatttcccgt aacccctcat cgatcgtaaa gattacgtgc cgatgtgtca ctttaaacat
    41161 atcattcgcc acaaccgctg accacctttg gctttcacct gttgcacacg ttggacagaa
    41221 ctttccacga catttcaagg gaacaaactt tgtttgatga catccctcgc agacaaacag
    41281 tcggtatcct ttttgaatat ctccacaata tctaaacttg tttacttccc gtttgacgac
    41341 tagccgaatc tttcgcccgt atttctcaac aaagttatcc caattatggt gttcgtcaaa
    41401 aaagattgct tcgagaatat tcgctttcat agtaatattt tacttgttca aaaacaaaat
    41461 gaaaagggct aaaagactgg tagtgcagcc tttttagccc ttaacttttt atactttctt
    41521 tactgtctaa aaaatatgcg gcgcacagaa cgcgccgcat atcggactaa gttttattca
    41581 aatttgtctt cgccaacaca atttgacgaa gagccttttc tttcaggaag acttcggtaa
    41641 tttttaggag ttctacctat tcttttaacc gtttattctc tttttctaag cgtgtaattt
    41701 catttctgcg aatggatttc atgacttcct ctaccgagaa aagtacgtta tccatatttt
    41761 tcaacttctt gaactcatca atacacgcta ttagagcgag cttctagcta tagactaact
    41821 tctttaacag aaaacccttc ttccgttacc agttttacag cggtaatttt aaatatttca
    41881 tcaaatgaac gtcttggcat aatccagatc ttcgctttct cttaatttgg tgtcaatttt
    41941 agtataaccc gtccaactta ttttttaact aagtgcccgg tttttcttga caactccacc
    42001 caaaagttag attttttggt ctaagtttta gggtcgcttc attactagaa ttcaacaaga
    42061 gatgtttttt attttattta aattatgggg tcagtttcca tttgattaca aacttctttg
    42121 ttttcttttt ttaaacgcaa taataaaggt tttaatttca gcctttttaa ttagaaaatc
    42181 aattttccca aattattttc tttagctttc ttaacgattt ctgtcgcaac taccacatct
    42241 aatacagccg aaccaactgt tttgaagatc gtgatttgtt ctccacttgt ccgaccgatc
    42301 aattcttcat tgatcagttg gcctagttcc ccatgatagc tgtctctttg aatataccca
    42361 tcttgcagcg gtgaaatgaa gtccccagct tcttttagaa caccgtccat ggtatcaaaa
    42421 ataacaacat ctgcggcttt gattatttct cgtggaattt cacacatttc cggtgtgtaa
    42481 gcaccgactc cattgatgtg cgcacctttt ttgacccact cagcagagaa tgtcgcacgt
    42541 tttgaagtgg tcacacttgt aatgatgtct gctccttcaa cgcattcttg actggttttt
    42601 gttgggcgca tcgttacaga aaattgttgc atcatttttt cagcaaattg gctggcacgc
    42661 tcaaaatcaa tatcaaaaat ccgaacttct tctaatttac gaacggtcaa cattgcttct
    42721 aattgtgact gtgcttgtcc gccggttcca atcagcgcgc caattttagc atcttctctt
    42781 gcaagaagtt ctgttgctgc tccttgaaca gcgcctgttc ttaattgtgt caaataagta
    42841 ccatccaaac aagcggatac catacccgtt tctggatcca atacgatcat cgttgctggt
    42901 acacttggta agtttttctt gatgttttca ggatacacgg aaacgatttt gacacctagc
    42961 gcgccttcgc caccttctac ataagcaggc atatataagc tttgtccgtt gctttttggc
    43021 acatcgatat tggtgcgaag aggcacactt gcctttcctg ctgaatacaa tgacagtgct
    43081 tttttgtccg catcgatggc ttctctcatt gaaaaacttt tgataatatc ttctttttta
    43141 agaaataaca tttgaatcgc tccttaatta gctgctgtaa ctttttttaa ttgactgccc
    43201 aacatcgtat atgcactgta tagtaagacc aaaatcacga tccatttcag gatataggta
    43261 tctaaagatt tgactaggaa tacagcgacc aataccccca atacaccgaa ggttgaagta
    43321 aaaagtgtga tttttctgct gtattcatta aatttaatga attgaacact gccgatcggt
    43381 actgagaagg tacaagcccc catcataata ggaaaggctg caactggatt caagcctagc
    43441 aagtagacag ttaccatcgt taacgcataa gaaccaatgc cgatattgtt caatgcacca
    43501 tagacaaaga gtaaaaatcc tgccaagatt aattttccgc catacaattc actggctgtt
    43561 ccgtttgatg gaatccagtt catttgtcct gcaaaaatca acaatgcagc aatgaccaat
    43621 cctatgccga caaacagttt gatcgttttc tctggtaact tcacgacaaa tcttggtcct
    43681 aaatatgcac cgataatttg gcagataata caaacagcta gtgtcttgat ccctacgttg
    43741 atggcagtga tgtaagacaa tgccatgacg gctaccggta ctacacattg ggtattcaac
    43801 gttccgggta gttttttcat gctgacccat ttcaactttg gataaaggac ggttccgata
    43861 gcaaagtcgg aaacgccaaa tgttgaaaga aagaagatga taaatgacgt gaagggcaac
    43921 agacgcatat cccctggttc ttctttgaaa ttttgtttgt ttttgaaaag gtctcttaga
    43981 aagtaaataa taaaatatag attgattgcg acaattagtg tcagcaaaat attccgaatc
    44041 ataaaaaatt cctcctacta gtaaatcttt ctgattccct caaagccctc accatatttt
    44101 gctttaagtt cttcaggaga ttctgcaatc acttttagtg tatatgcatc atgtttgaca
    44161 tattcttttg ctagattgac catggcgtgg ttttttgtcc aagtgacatc ggttgattgc
    44221 agttcttcgt taatggtacc aaacacgact tcttgctcat cggcgacgat gttgatccat
    44281 cttgctccaa agtcagcaag tttgtctgtt tcaaaaccat gaatataata cttttctaag
    44341 ggaagatcat attcatgggt agaactgaat aaaatcagta caaacttatc aactcttttt
    44401 tcagcttgct gcaatgcctt tagtagtgaa tccgtgaaat tctcatgcca aacttgaatg
    44461 agcaaacttt cttttgcgtt tttcaccaaa tgctctactt tatccagaac atactcgata
    44521 ccatcaactt tccacagcat ttcttcttca tctttttctt tgatcatccc tagattcttg
    44581 gtaagaaaag aaagatcatt tttaacactt ttttctaatt tctctaaaaa ctcatttgcc
    44641 gagatggcat gataaagttt aggttctgat ttattaacga caatcaaatt cttttttaat
    44701 agtttttcca aaatattata tactttcgag cgtggcacac ctgagatttt acttacttca
    44761 tatcctgtaa gattaccttt ttctaatagt gtggtataca ctcttgtctc catctctgaa
    44821 aaatcatatt ttttcatgat ttgaataatc gtttccattg attaatcctc actttatttt
    44881 tagtagctac tacggtaaca actataaatt ctttttgatt atctgtcaag agttagttca
    44941 ataacacaca tataataatt agcttaaaaa caatcttttt attaaaaaga aacattataa
    45001 tatgaatgtt tgtgaaaaaa taatacaact tagtcattct ttacaacgat ttatagaaaa
    45061 acaacctctt taaatataaa aggtacgggt tactgattac ttgtaaatct ttacgttcca
    45121 gttttattct ttctaaaaaa tatactcact tcttttctta gacttctttt tttgattctc
    45181 ctctttatgg tcttgtcctt ttttgccttc aaaatattta aataaacccc tttcaaaagc
    45241 atttctaggt tttggacaat aaggaatgac ttttttcttc atataaatac ctcatttaac
    45301 tatttgatat catcacatta tcaaaaaaaa taggtttttt attaaaacta tcttgacctt
    45361 tcgggagatt tcactgtgat ttatttttat tcccatactt ttttcgttgg tcactttcgt
    45421 atacctaatc taagcgatca tcggcgacgg tgcttttaca acgaatggca ggacaaaaca
    45481 aggggcggaa aaactttttt gcctatttca taaaaaactt tttcctacag caatcccttg
    45541 ttttgtccta ccacacgttg tgaacccaaa gcacgccgac gaacgcttag ctatacgaaa
    45601 atgacctcca aaaacaaaaa agtgaggtga atagaaacaa aaagaaattg aaatctccca
    45661 aaatgtcgtc cgcctcggag acaacttgct gctgaaaatg gttggttaat tcatttaagt
    45721 attaaataag ataaatcatc aaattaatcg gaaaaatagc atgattggcc ttatcaaaaa
    45781 taaactgtaa aaacttatat ttctatgagt ggtggaacaa tgctaatacc aaattaattt
    45841 atcgattacc cacctgcaca aaccaaagat agcattatta ggaaacgaat caatcagcga
    45901 atccagcatt ataatgagga tactaaaaat aaagttttca taattataaa tccgccttcc
    45961 tacgaaaggc accaatgagg tgatgaaaac cttagagcgc tttctttctt tttttattcc
    46021 gtgttaccct ttccttgcag gtccctggta cactacagaa cccgaggagg tgagtggcgg
    46081 ggctgtatag cggcaacccc gcataattat atggtgtcgg tcatccgtta aggcatcaat
    46141 gaatggcgca aacccgtagc ccgtaaactt atctcttccg aagttgactc gatttcgccg
    46201 gatgaccagt ccctaccaaa cctgtactat ttttttacag gaggttgcta tgttcaaaat
    46261 ctttcgtaaa aattgttgtg gcttggatgt ccacaaaacg tggatctatg cgtgtatcgg
    46321 aatcacagat gcgaatggtc gtacagtgta taagcaagct cgcttctctt cctttacaaa
    46381 aggtctcaat gaattagctg aatggcttgc taaatacaac tgtaaggatg tgtgtttgga
    46441 atcttccggt aaattttgga ttcctgtttt taatattctt gaaaaaactt gttttgtcac
    46501 actggctcat ccgaagtaca ccaaacccca gaaaggaaat aaaacagatc gcaaagatgc
    46561 aaaaatggat ttgtgatctc tttatgtgcg acatgatcaa acccagtttt attcctggtc
    46621 ttgaaatccg ccagcttcgt gatctaatgc gttatcggac gaaactgacc aacatgaaaa
    46681 caagtgaaaa gaatcgtgct ttaaactgct taaccgtttc taatctgaaa ctagatgatg
    46741 tttttagtga tgtgttcgga aaatcatcca cttctatcat ccaatacttg ttagaacatc
    46801 ccggtgaatc atttgatgtt cgaccattta tccatgcccg ttgtaaaact cccgttgaag
    46861 aaatccaagc ggcagttgag ggggcgattt ctgtacctca agcgattaag cttcgccaat
    46921 gcctcaacca tattaacgag attgagaagc atcgagaaga gatcaatcta gagattcaag
    46981 ctcttgcaga acccttctcc cctgttttgg aactacttcg taccataccg ggcttagaca
    47041 ctcagccaat aacagcgatt gcgattcttt cagaaatagg accggatatg tccgtgtttc
    47101 caacttcgaa acactttatt tcttgggctg gatgctgtcc aagaaacgat cgcagtgccc
    47161 gacgaacaaa atcaacacgt atctctcgtg cagggcagtc gttgaaaccg ctacttgtac
    47221 agattgccaa tgctcttctc agatccaaag aacatccaga atttaaaaat cgctatcgta
    47281 gaatcaaagc gaatcgtgga cacaagaaag ctatcattgc tatttgtaag atgctcctga
    47341 ccgctatctg gaatgtctta tcaaaggtgg aaccctattc tgctaaagga tatctggagc
    47401 caaaacctgc gaaagaagag aaagttctga cgcaatccca agctctggac cttttaagaa
    47461 aacgcggata taccattata gatagtgctt aaaccaaagt tacgttgatt cttcctaaat
    47521 agactcgttg tcttccattt caaaaatttt gaaaacggta ggcttattta gtgtaccgtt
    47581 cttcaggtat cttcaaatag atttattttc aaacttattt cctttcttct tttactctcc
    47641 tatagcttag catttaagtg ggcactcgtg tcaccttgct agatagctat gaatgccgta
    47701 ataagcctcc tagtatatag gcagactgcc aattataact gattcaatcc acattatgat
    47761 aaaatccacc ttttcgtttc atatgtgttg aaatatcaca tatgtcaaaa gtgcacgagt
    47821 cttttcattc agtgctttgt aaatactcaa aattattcct tttcacttta aaaaaaagat
    47881 aattagtaca aaatcagaaa tgaggaaagt attaggatgg actagaaagg agttgttgaa
    47941 tttgttgcaa cagattggtc atttatcaat taaaaattag gtgaagtttt attcatcact
    48001 gcagctgctg tttcccagag aataaaacgt ctagaagaaa ctggtcagtt actgtattat
    48061 caagcagcaa ttgactacga acaagcttga ttactaacca aagcgtttat ccacctgttg
    48121 attaaatcaa atcaaaaatc aggtttttat acgttcataa aagcaatctc cagtgtactc
    48181 gaatgtgact gtatcatagg tccttattcg atgttaatca aaatggggtt tccttctacc
    48241 gaatcattgg acagttttat caatgattta tcaaagtttg gacgaaccaa cacccaaatc
    48301 gttttctcga ctcttgtttc cttaatggtt tatcagttct ggaatgttta tcatgcgctt
    48361 ttattaaaat agaaaagagc ttttttcatg cgacaaagca gtgatcattt attggcacat
    48421 gcaatctttg tggatccatt ttttgaaaat acgatattta gttttgaccc aaaaatcttt
    48481 aaatacgtat ttgataattt atcttaacaa ccagataatt ttattcagca ccgtattatc
    48541 tatattgaat tctcggaatc aagataaatt tttttccgtt tttgtttctt ttttaatctt
    48601 cttcttgtta ttaatagtga ttaggatgat attcctatat tgatctttga taattagata
    48661 taatttttga ataacgttcc ctgctattcg agctagaaat acaagtacgc catgacctag
    48721 ttagcgacaa ctacttagag atttcttttg ttctgggaca acaaaatgcc atgacaatag
    48781 cagtaaataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg aaattcctat
    48841 gtgcttaccc aaagcggttc aaaaatttta aactattatt ttaggagatg attttataaa
    48901 atcgaaagcc tacttgaata aaaaacaagc ctgtgaattt ctaggaattg ataataatac
    48961 tttagataag tggattcgtg tatataattt tccagtaata aaaattgatg gcgtagttcg
    49021 ctttgacgta gaggatatca aagatttcat gaatacattc aaaaaaaatt gaaattttct
    49081 tctcgtcaca tatactataa atagcgaaat gtactgcctt actttttaaa ggagtgagga
    49141 agtttgccta caaagctttc aaatgggaaa tataaaacta atctgaggta tccaaaaaaa
    49201 ttcagagaaa tcactggaat tgcatcagag aagtatcaaa aagtatttcc aactaggcaa
    49261 ttagctatta aggccgaaag ggaaatgaaa aagaagattg aaacagtttt aagagaggag
    49321 aatgcaaact cactagaatt gaaaggaaaa ataaaattta aagattttta tgaatcaaaa
    49381 tggcttccta gatatgaact aggtcaaact actaggtcaa acagggctcc aagtaatgta
    49441 acaattagta atactaagga tatattccga ttgcacatcc ttccaatgtt tggtgaatat
    49501 gcaatgaatt atctgaattc aaatactgag ataatatcag atgaattaac aaagaaatcg
    49561 aaagaatacg caaatataaa aattatcaaa ggctatgttc gctctatgtt tgatattgcc
    49621 gaaatactat gttatatcga attcaaccga actaccaaaa ttattcaatg tattaccgcg
    49681 cctaagaaaa tggctttaga agaaaaaagg ataagggaag ggaatcaagc attatcatca
    49741 gaagagctga ctgattggat agaagcagta aacaatgact taaataatca cttgcttaca
    49801 cttcacgatt acacactttt catgcttacc ttatatctag gtgatagaaa aagtgagact
    49861 tatgctctgc aatggaaata tattgatttt aataaaaata cggttcgtct caaatatgct
    49921 ttagataaat atcaaagaaa gacacataca aaaggttgga aagacaccgt tattcaggca
    49981 ccagaagttg tgatgacatt attaagagaa tggaaatcag ctcaggctga acaattgttg
    50041 aaactaaaaa ttatacaaac acctgaccag tacctcttta cctattccaa accttctgga
    50101 gaagtaaatt gtcctgttca cgctgactac ttaaactatc gaatcaatgc aataaaaaaa
    50161 aggcatcctg aattggctca attatctcca cacaaacttc gtcataccta tgcaactata
    50221 gctagggaag gtggagcaaa tatgaatcaa atctctaatg ctttaacgca ctcggatatt
    50281 tctactacaa aaatatacgt gaacacacct gacgtagttg ataaatctgt ttttgaagca
    50341 tttcaaaaag gattgaataa gtgtgattaa catgtgttct caaatgtgtt cttattataa
    50401 aaaaagacac tttccaaata attgaaaagt gtcctgaaat attgatataa aaagaatatt
    50461 aacgttttga gaattgtgaa gctttacgag cttttttaag acctggtttt ttacgttcga
    50521 ccatacgtgc gtcacgagta agtaagccag cacgtttaag agctaaacgg aagtcaggat
    50581 caacttctag caatgcacga gcgataccat gacggatagc tcctgattgt ccagcgtatc
    50641 ctccgccgtt tacgttaaca aatacgtcat atgcgccttt tgtttcagta acagcgaaag
    50701 gttgattgat tacttcacgc aagtcagcat gtggaatgta ttcttcaaca tcttttttat
    50761 ttacagtaat tttgccagtt cctggtacta gacgtacgcg agcaactgcg tttttacgac
    50821 ggcctgtgcc gatatattga acttgtgcca atgaaattcc ctcctattag attaagtttg
    50881 tgatgtctaa aacttctggt tgttgtgctg catgtggatg ttctgctcca ccgtatacgt
    50941 tcaattttgt aaattgtttg cggcctaaag tgtttttagg aagcatacct tttacagaag
    51001 tttcgatcaa acgacgagaa tttttagcac gcaattcgcc agcagtgatt gatttcaatc
    51061 ctcctgggta gttgctgtga cggtaataaa ttttgtcagt cgctttttta cctgttaatt
    51121 tcactttgtc tgcattgatg acgatgacga aatcgccagt atccaaatga ggtgtgaaag
    51181 ttggtttatt tttaccacgt agtacagatg caacaactgt tgaaagacgt cccaatggaa
    51241 catcagttgc gtctactaca taccatttac gttctacttc gcctggtttg gccatatatg
    51301 ttgtacgcac gttattttcc tccaatttaa gttctgatgt ttgacataca cttcgagttt
    51361 ccggggctct ttgtggggca aacaatacca tttaacatat tacccgctaa tctagtcaat
    51421 gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac tttacttctt ttctttcttt
    51481 tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct
    51541 tgtcacaacc ccttattttg aataaacggt gttctcctgc caactcctcg gtcacaagtc
    51601 acaatatcca taaaacataa gaagctgttt tttaaaattg tttcttgtga ctcggagctg
    51661 aacggcaggc t
//
LOCUS       DACEOM010000010        50247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEOM010000010 DACEOM010000000
VERSION     DACEOM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.011"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            476..1183
                     /locus_tag="IW781_000580"
     CDS             476..1183
                     /locus_tag="IW781_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1181600.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1243..1887)
                     /locus_tag="IW781_000581"
     CDS             complement(1243..1887)
                     /locus_tag="IW781_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1181601.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2014..2301
                     /locus_tag="IW781_000582"
     CDS             2014..2301
                     /locus_tag="IW781_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181602.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2409..2729
                     /locus_tag="IW781_000583"
     CDS             2409..2729
                     /locus_tag="IW781_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1181603.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2810..4123
                     /locus_tag="IW781_000584"
     CDS             2810..4123
                     /locus_tag="IW781_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1181604.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4120..4839
                     /locus_tag="IW781_000585"
     CDS             4120..4839
                     /locus_tag="IW781_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1181605.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4967..5518
                     /gene="raiA"
                     /locus_tag="IW781_000586"
     CDS             4967..5518
                     /gene="raiA"
                     /locus_tag="IW781_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1181606.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5740..8274
                     /gene="secA"
                     /locus_tag="IW781_000587"
     CDS             5740..8274
                     /gene="secA"
                     /locus_tag="IW781_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1181607.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8400..9501
                     /gene="prfB"
                     /locus_tag="IW781_000588"
     CDS             join(8400..8462,8464..9501)
                     /gene="prfB"
                     /locus_tag="IW781_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1181608.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9606..10292
                     /gene="ftsE"
                     /locus_tag="IW781_000589"
     CDS             9606..10292
                     /gene="ftsE"
                     /locus_tag="IW781_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1181609.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10276..11169
                     /locus_tag="IW781_000590"
     CDS             10276..11169
                     /locus_tag="IW781_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181610.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11321..12769
                     /gene="cls"
                     /locus_tag="IW781_000591"
     CDS             11321..12769
                     /gene="cls"
                     /locus_tag="IW781_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1181611.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13106..13963
                     /locus_tag="IW781_000592"
     CDS             13106..13963
                     /locus_tag="IW781_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1181612.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14078..14998
                     /gene="pstC"
                     /locus_tag="IW781_000593"
     CDS             14078..14998
                     /gene="pstC"
                     /locus_tag="IW781_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1181613.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            14998..15882
                     /gene="pstA"
                     /locus_tag="IW781_000594"
     CDS             14998..15882
                     /gene="pstA"
                     /locus_tag="IW781_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1181614.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15897..16703
                     /gene="pstB"
                     /locus_tag="IW781_000595"
     CDS             15897..16703
                     /gene="pstB"
                     /locus_tag="IW781_000595"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181615.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16719..17477
                     /locus_tag="IW781_000596"
     CDS             16719..17477
                     /locus_tag="IW781_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181616.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17490..18167
                     /gene="phoU"
                     /locus_tag="IW781_000597"
     CDS             17490..18167
                     /gene="phoU"
                     /locus_tag="IW781_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1181617.1"
                     /translation="MLRTQFEEELLNLNNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18405..19436
                     /locus_tag="IW781_000598"
     CDS             18405..19436
                     /locus_tag="IW781_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1181618.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19433..20200
                     /locus_tag="IW781_000599"
     CDS             19433..20200
                     /locus_tag="IW781_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1181619.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20300..21868
                     /gene="liaX"
                     /locus_tag="IW781_000600"
     CDS             20300..21868
                     /gene="liaX"
                     /locus_tag="IW781_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1181620.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22168..22485
                     /locus_tag="IW781_000601"
     CDS             22168..22485
                     /locus_tag="IW781_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1181621.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22485..22835
                     /locus_tag="IW781_000602"
     CDS             22485..22835
                     /locus_tag="IW781_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1181622.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23002..23940
                     /locus_tag="IW781_000603"
     CDS             23002..23940
                     /locus_tag="IW781_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1181623.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23955..24785
                     /locus_tag="IW781_000604"
     CDS             23955..24785
                     /locus_tag="IW781_000604"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1181624.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24829..25854
                     /locus_tag="IW781_000605"
     CDS             24829..25854
                     /locus_tag="IW781_000605"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1181625.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25869..26807
                     /gene="galU"
                     /locus_tag="IW781_000606"
     CDS             25869..26807
                     /gene="galU"
                     /locus_tag="IW781_000606"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1181626.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26932..27372
                     /locus_tag="IW781_000607"
     CDS             26932..27372
                     /locus_tag="IW781_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1181627.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27375..27977
                     /locus_tag="IW781_000608"
     CDS             27375..27977
                     /locus_tag="IW781_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1181628.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(28036..29142)
                     /locus_tag="IW781_000609"
     CDS             complement(28036..29142)
                     /locus_tag="IW781_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1181629.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            29352..30353
                     /gene="ccpA"
                     /locus_tag="IW781_000610"
     CDS             29352..30353
                     /gene="ccpA"
                     /locus_tag="IW781_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1181630.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(30690..33035)
                     /locus_tag="IW781_000611"
     CDS             complement(30690..33035)
                     /locus_tag="IW781_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1181631.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            33314..34216
                     /locus_tag="IW781_000612"
     CDS             33314..34216
                     /locus_tag="IW781_000612"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1181632.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            34429..36204
                     /locus_tag="IW781_000613"
     CDS             34429..36204
                     /locus_tag="IW781_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1181633.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(36270..37856)
                     /locus_tag="IW781_000614"
     CDS             complement(36270..37856)
                     /locus_tag="IW781_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1181634.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLAGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            38107..38331
                     /locus_tag="IW781_000615"
     CDS             38107..38331
                     /locus_tag="IW781_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1181635.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            38473..40224
                     /locus_tag="IW781_000616"
     CDS             38473..40224
                     /locus_tag="IW781_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1181636.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            40224..42008
                     /locus_tag="IW781_000617"
     CDS             40224..42008
                     /locus_tag="IW781_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181637.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            42334..43185
                     /locus_tag="IW781_000618"
     CDS             42334..43185
                     /locus_tag="IW781_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1181638.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            43239..44990
                     /locus_tag="IW781_000619"
     CDS             43239..44990
                     /locus_tag="IW781_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1181639.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            44994..45164
                     /locus_tag="IW781_000620"
     CDS             44994..45164
                     /locus_tag="IW781_000620"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181640.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(45204..46106)
                     /locus_tag="IW781_000621"
     CDS             complement(45204..46106)
                     /locus_tag="IW781_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1181641.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            46337..47740
                     /locus_tag="IW781_000622"
     CDS             46337..47740
                     /locus_tag="IW781_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1181642.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            47740..49173
                     /gene="ascB"
                     /locus_tag="IW781_000623"
     CDS             47740..49173
                     /gene="ascB"
                     /locus_tag="IW781_000623"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1181643.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            49318..50244
                     /gene="trxB"
                     /locus_tag="IW781_000624"
     CDS             49318..50244
                     /gene="trxB"
                     /locus_tag="IW781_000624"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1181644.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
BASE COUNT        16243 a         8803 c        10774 g        14427 t
ORIGIN      
        1 tttttttgct ttgctacctt gttccttgaa gattctgctg atttaagata gagttaagtt
       61 aactacaaga attggaggga tccacatgaa atggaaagaa aaagtagacg aagcagctga
      121 aaagttatat gatttggtca aatcagaaaa atacaatatt gaagtgaata ttccgaaaaa
      181 aggcggaaaa gcggtccagg taaaatcaaa acgcccaacg aatcatacaa aaaaatggat
      241 ggctaaaaac cgctaataat aacaaaagat ccataagtcg gcattttcat cgattttagg
      301 gtcttttgtt attttctagg ttgaacatat ctaaaaaatt gtttctgaca ataggagtag
      361 gaagaacgat gtgtgtctaa atcctctgtt gatggataga aacctttatc tttttcagtt
      421 gtgaatcagg tatataccag ttataataga agaaacgaaa ggacgggtgt acgttatgtc
      481 gaatcaatta cctgtatata tacagattca tgaccaatta aaaaatgaaa tcgaacaagg
      541 tatatggaaa gtcggagacc gtcttccttc agaaagagaa ttagcagtga aattcaatgt
      601 cagccgtatg acgttgagac aagcaattca aaacttggca gatgaaggaa tcttagaaag
      661 aaagatcggt tcgggtacat atgttgcgcg agaaaaagta caagaaaaaa tgtctggtgc
      721 aacaagtttt acagaaatta tggaatcgca aaatcgagta ccgtctagtc ggacaatctc
      781 ttatttcttg acttcgccaa gctcaagtga aatggaaaaa ctaaacttga gtaaagatga
      841 cacgatcttg cgaatggaac gtattcgttt cgcagatgat gtccctattt gttttgaagt
      901 tgccagtgtg ccacaaaagc tgatcgatgg ctacagcaaa gcagagatta ctgaatcgtt
      961 gtatcgcacg ctagaagaaa aaggcgggaa aaagatcggt ccagcgaatc aaacgattac
     1021 tgctgtgttg gcttctgaga aaattgctga ctatttagag gtaaaacgag gagatgcagt
     1081 tcttcgtttg cgccagattt cttattttga agacggtacg ccgtttgagt atgtgcgtac
     1141 gcaatacgta ggaaatcgtt ttgagtttta tttggaaaaa taaaaaacag gtgatccctg
     1201 attccttact tagaaataaa agagaatcag aatcacctgt ttttatatat gatcagttac
     1261 cagttgctcg tgataagaaa caggaccctc ttcaaattgg acttgtccgt tcagcagctc
     1321 aatgatggat tctttaaagt ggtcagcact tttttcatct acagcacaga tgacgctaac
     1381 ttgatcagta tattggattt cttttataaa aaaatgttgg agttcaagat aattctgtaa
     1441 ttttcctagt tgcggatacg tgatcatcac agagatctcc tgttgcaagg taccttctac
     1501 gattccaatg tcagacaatg cttgagagac agcatgagtg tatgctcgta ttaatccgcc
     1561 tgtgccgagt ttcgttcctc caaaatatcg agtgactact gcagctacat tgatcagttg
     1621 attttttttg aggacttcca acatcggtac gccagccgtt ccacttggtt ctccatcatc
     1681 gctgcttcgc tggatttcat ttttttctcc aatgatgaag gcgctgcagt tatggttcgc
     1741 tttccaatgt tcttttttta tttcttgaat gaaacttttt gcttggtcct ctgattctat
     1801 tcgtctaaga gaacagataa agcgtgattt tttgatctca atctcacttt gtccattttc
     1861 ttttattgta cgatagcgct cgatcatctt gtcacctctt tttttcattt tctttattca
     1921 gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaatcg ctgtagtatg
     1981 cgataataaa gacaaaggta ggtggatctg gggatgcagg aacgaatgaa aaagtacgat
     2041 gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac ctttacccaa
     2101 tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga aagtacagat
     2161 tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat aaatgcaggc
     2221 tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa attagtgaaa
     2281 tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa tttctttctt
     2341 tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt taaaaaggag
     2401 agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg agcacaagtt
     2461 tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactcg gatattttcg
     2521 ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agatgtagat gtactgttat
     2581 taggacctca agttcgcttt atgaaagctg attttgaaaa acgtctggaa ccaaaaggca
     2641 ttcctttaga cgtcatcaat atggctgatt atggtatgat gaatggcgaa aaagtgttag
     2701 atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga acaagcgatc
     2761 caaaaaatag atcgcttgtt ttttcgtata tttttttagg gcgaataata tgaatcattt
     2821 ttggggtaga aggcttctga cgagagatat taaagcaata gactgtgagg aggtaaattt
     2881 accaaattat gagaagatcg cagcagtgga acgtttgggc aactggattc tttgtcatcg
     2941 ctgtggaatg aggacgtccg tcattgatgg acagcttgca gattacggtt atttttgtac
     3001 acattgtcta tatttagggc gatgcgacac tcaacaagat ttatatttgt ttgatcagcc
     3061 tgaagcaaaa cctcgagaag tcgtcttttt ttggacagga cagcttactg aaaaacaaac
     3121 ggaaatcgca aagagaatac tttgccattc agaaaaaaga catcatctta tttgggctgt
     3181 aacaggtgca gggaaaacag agatgcttta tcctatttta gtggaaactt taaaagctgg
     3241 aggaagagta gcgatttgta caccgcgagt cgatgtttgt aatgaattgt ttttacgcta
     3301 tcgttatgtt tttccagaag aaaaaatcac gttgctccat gggaatacag caacggctta
     3361 tacctttact tcttttgtta tctgtacgat tcatcagcta taccgttttt atcggtcctt
     3421 tgatttaata gtgatcgatg agatcgatgc gtttccttat aacggggatg caggattagc
     3481 tcatgctgtg caaacagcga ttaaaccaga cggacgattc atctacttaa gtgccacacc
     3541 tgataaaaag ttactaaagg aaatcaagca gacgtttgac atccacaaac ttgctttgcg
     3601 ctttcatcgg cgtttacttc ctgaacccgt cttggttttc atgaataatt ggcgacaaaa
     3661 atgcaagaac aagaagaaga taaaatcttt ggtgcggatc attcatcaat taattaaaga
     3721 aaaccatatc cttttatttt gtccaagtat cacgatgatg catcgtttga aagccacact
     3781 tcaagaggct cttccgagat atgagatcac agatgtttct tcaagagaca atcagcgggc
     3841 ggaaaaagtc cagaaaatgc gagaagcaaa atatgacata ctgttaacca cgatgatcct
     3901 tgaacgtgga gtcacatttg aaagaatatc cgttatcgtt ttaggagcgg atcatccggt
     3961 ttttactaaa tcttctttgg tacaaatcgc aggaagagcc gatcgtaaag ggccttatac
     4021 gaacagtcaa gtatactttt tctatgaaga aaaaactgca gctattcgaa aagcttgtca
     4081 ggaaatcaaa gaaatgaatg aagaagggaa gaagatgctg tgaggtgcag ttgctgcagt
     4141 aaagatatcg tccaaaattt aacattcagc gagattattg gtgtttcgaa atacagatta
     4201 cctcagactt gttcagtttg tcgcgcacaa tttcatttat tggaaacaag aggctgttct
     4261 ggctgtcaaa aaccaatcca tttcaatcta gagagtacga aaaagaaagt aaagtctatt
     4321 cccgtttact gcagtgactg tcaagaatgg aagaagaaat atccgaccta tgattttcag
     4381 caccgtgcct tttttgtgta cgatgaaaat atgaaggcct ggctcaatca atacaagttt
     4441 atgggagata tccgactagc aggcaccttt gcatataaat ggacgcagat aaaaaaagaa
     4501 tacccgacat acatctattg tccaatacct ttatctgaaa aaaggcgaac agaaagagga
     4561 ttcaatcaag taagcgagat gttgaaagca gcaagaattc cttttcagat gctactcaaa
     4621 agagagaagc atctgctggc tcaagcaaaa aacacgcgaa aggaaagatt ggagatgcct
     4681 caaccttttg aacttgcagt caccttttct aagatacaaa ataaaaaaat attgattatt
     4741 gatgatgtct atacaacagg acagactctt ttccacgcag cagattgttt gcaggtcgca
     4801 cagccgaaaa tcatccgaac catttcttta gcaagatgaa ttagatgtgg aaatttgata
     4861 gcgatttaat ttgcaaataa cctagagttc gatataatag aattaagaag agggaaagag
     4921 tggtccttct ccttcttgat tgtggtagac gaaaggggta atttttatgt ttagatataa
     4981 tgtacgcggc gaaaatatcg aagtaactga agcgattcgc gactacgttg agaaaaaagt
     5041 aggcaaacta gaacgctatt tcagtgacgc acccgatgca acggcgcatg tgaacttaaa
     5101 agtttataca gaaaaaacag caaaagtgga agtaacgatc ccattacctt atcttgtatt
     5161 acgtgccgaa gaaacttctc cagatttata cgcaagtatc gatttggttg ttgacaaatt
     5221 ggaacgccaa atccgcaaat tcaaaacaaa aattaatcgt aaatcccgtg aaacgggcat
     5281 gaatactgca gatgctactg ctttattcaa tgaagaggac gaaaacgaaa atgattctga
     5341 attagatatc gtacgtacga aacgcctttc attgaaacca atggatagtg aagaagcagt
     5401 cttacaaatg aacatgctgg gacataactt ctttattttt gaagatgcag aaacaaatgg
     5461 tacaagtatc gtttaccgcc gtaaagatgg aaaatacgga ctgattgaaa cagactgatc
     5521 agccttactt aaataacaac gaaaacttgc agcagctact gacagctgcc gcaagttttt
     5581 ttgagtttcc tcctctttta gatgaaatat gaataaatta tgaagttctt actttaatac
     5641 gcttaagata tttcaattat ctataactaa tgataaaatg aaaagtaaag tctacatagt
     5701 ctggataata cacagaagga aaaggagatt acacaacgca tggccaattt gattcgttca
     5761 cttatcgaaa acgataagaa agaactaaaa cgtttagata gcattgcaaa aaaagttgaa
     5821 gcatacgctg accaaatggc agcattaact gatgaacaat tacaggcaaa aactgctgaa
     5881 ttcaagggca gataccaaaa aggagaaaca ttagatcaat tacttccgga agcttttgca
     5941 gtcgtccgag aagcagcaaa acgtgtattg gggttatatc cacaccacgt acaattgatg
     6001 ggtggaatcg tgcttcacga tggaaatatc cctgaaatgc gtactggtga aggtaaaacc
     6061 ttgaccgcaa cgatgccagt atatctaaat gctctgactg gcgaaggggt acacgtagtt
     6121 accgtcaatg aatatttagc aacccgtgac tcgacggaaa tgggcgaact ttataatttc
     6181 ttaggattaa cagtggggtt gaatatcaac tcaaaatctt cggatgaaaa acgcgaggct
     6241 tataattgtg atatcactta tagtacgaat aatgaattag gatttgacta cttgagagac
     6301 aatatggtcg tttaccgcca tcaaatggta caacgtccgc tgaattatgc cattgtcgat
     6361 gaagttgact caatcttgat tgatgaagcg agaacgccgc tgatcatttc agggcaagca
     6421 gaaaaatcca cagctttata tacaagagca gacaacttcg tgaaacgttt gaaagaagaa
     6481 gaagattata aaatcgatgt acaatcaaaa acaatcagtt taaccgaagc agggatcgaa
     6541 aaagcagaag aaacattcgg tttggataac ttatacgaca ttgaaaatac tgcattgacc
     6601 catcacttgg atcaagcatt gagagccaac tatatcatgc ttttagatat cgattatgtt
     6661 gtccaagacg gaaaagtact gattgttgac cagttcactg gtcggatcat ggatggacgc
     6721 cgttattcag atggacttca ccaagcaatc gaagctaaag aaggcgtaga gatcgaagac
     6781 gaaacgaaaa ccatggcgac gatcactttc cagaactatt tccgtatgta caagaaactt
     6841 gcgggaatga ctggtacagc taaaacagaa gaagaagaat tcagagaaat ttataacatc
     6901 caagtatacc agattccgac aaatcgtccg gttatccgtg atgatcgtcc agatttgctt
     6961 tacccaacat tacaaagcaa gttccacgct gtcgtgcaag atatcaaaga tcgctaccgc
     7021 aaaggtcaac ctgtgttagt cggaactgtt gcggttgaaa catctgagtt attatcagat
     7081 atgctgaatc aagaacgtat tcctcatgaa gtgctgaatg cgaaaaatca ctttaaagaa
     7141 gcagagatca tcatgaatgc tggtcaaaaa ggtgctgtga cgattgctac caacatggct
     7201 ggtcggggaa cagatatcaa gttaggactt ggggtaagag aacttggtgg tctagcagtt
     7261 atcgggacag aacgacatga atctcgtcgt atcgataacc agcttcgagg acgtgcagga
     7321 cgtcaaggag accctggtat gtcacagttc tacttgtcat tggaagatga tttgatgaaa
     7381 cgattcggtt cagaacgaat caaatctttc ttagatcgga tgaagatcgg tgaggaagat
     7441 gctgttatcc aaagcaagat gctgacgaaa caagtagaat ctgcacaaaa acgtgtagaa
     7501 ggaaataact atgacacacg taaaaacgtc ttacaatacg atgatgtgat gcgtgaacaa
     7561 cgtgaagtaa tctataaaca gcgacaagaa gtaatcatgg aagaaaaaag cttatccaag
     7621 ccactaatga acatggtcaa acggacaatc agtcgtgtag tagatgcgca tacacaatta
     7681 gagaaagaaa attggaattt ggaaagtatc atcgattttg ccggcagtac tcttgtccat
     7741 gaagatagta ttactcttgc agatatcgaa ggaaaaacag ctcaggagat caaagagtat
     7801 ctaaacgaac gagcacaaga agtatttaat accaaagcgg ctcaactaaa tggtccagag
     7861 caactgttgg aatttgaaaa agtcgtgatc ttgcgtgttg tggactctaa atggacagac
     7921 catattgatg caatggatca attacgtcag tcaatcggct tacgtgctta tggacagaac
     7981 aatccattag tcgaatatca aacagaaggc tataagatgt ttgaagatat ggtaggcgcc
     8041 atcgagtatg aagtcactcg aatctttatg aaagctgaga tccgtcaaaa cgtacagcgt
     8101 gaacaggttt ctcaaggaac agcttctcac tcagaagacg gagatgccaa agaaaatagt
     8161 aatactagcg cgaaaaaaca gccaatccat gtgaacaagg tcggaagaaa tgatccttgt
     8221 ccttgtggaa gcgggaaaaa atataagaac tgtcatggaa aaaatgcaaa ctaatttctg
     8281 acagcaggat tggtgagaca aattcttttt ttaagaattc gtctcacctt ctttctgtta
     8341 tttgctgatt ctgttagact agatataatg aaaaaagtat agtgaaagaa ggaaaaaaca
     8401 tggaaatatc cgaaattcga aatctcctga cagaaatgca ggaaaaggtc acaagcttca
     8461 ggaggtctct ttgacttaga ccagctggaa gaagacattg cggaagcaga agcaaaaatg
     8521 gctgaacctg gtttttggga tgatagcgaa gctgctcaaa aagtgatcag tgaaaacaat
     8581 gcaaataaag aaacttatga tcaattccat tctcttgctg aagaactgga agaaatcgaa
     8641 ttgatggtgg agatgttgca ggaagaacct gatgcagcca tgcaagaaga agcagaaaca
     8701 cgtatcaatg cgctagatga aaaaatgaag acatatgaat tatctatgtt gttagatggt
     8761 ccttatgatc gaaacaacgc catcgttgaa ttgcatccag gggctggcgg aacggagtcc
     8821 caagattggg gcagtatgct tttacgtatg tatacacgct gggcagagca gcatgggtat
     8881 caagtagaga cactagatta tcaagcaggt gatgaagcag gaatcaagag tgtgacccta
     8941 ctgatcaaag gacacaacgc atttggttat ctgaaatcag aaaaaggtgt tcatcgcctt
     9001 gtacgtatct cgccttttga ctcagcgaaa cgacgtcata cttctttttg ttcggtagat
     9061 gtgatgccag agcttgatga aacgatcgat atagaaatca atccagatga tttgaaaatc
     9121 gatacgtacc gagcaagtgg tgcaggtggt cagcacatca ataaaacaga atctgctgta
     9181 cggatcactc acttgcctac aggaacagta gtagctagtc aggcacaacg ttcacaattg
     9241 aaaaaccgtg agcaagcgat ggggatgtta aaagctaagc tttatcaatt agaagtggaa
     9301 aagaaagaac aagaagcagc tgcacttcga ggagaacaaa aagaaatcgg ctggggttca
     9361 cagatccgtt cttatgtttt ccatccatat tctatggtaa aagaccatcg gacaaacgta
     9421 gagacaggaa atgtccaagc agttatggac ggtgaccttg atttatttat tgacggctat
     9481 ttaaaaatgc atctacaata agaacccatt gttaaattga aataaaattg taagattttg
     9541 caagcatgtt gaaacattgc catgctataa tagctaaaga ctgaaaaaga tatggagaga
     9601 acgccatgat tgaaatgaag gatgtaatga agaagtactc aaatggtacg accgctatcc
     9661 gcaatctctc aattgtcatc gaacaaggag agtttgtcta tgtggttggt ccgtctggag
     9721 ccggtaaatc tacattcatc aaattgatgt atcgagaaga aaaagcttca aaaggaactt
     9781 tgaacgtcgc aggtcatgat ctaatagcaa tcaaaaaccg cgaagttcct tatctacgca
     9841 gagaaatcgg cgtggtgttc caagactata aattattacc aagaaaaaca gtatatgaaa
     9901 atgttgcgta cgctatgcaa gttatcggca gaaaacctcg ggatatcaaa aaacgagtaa
     9961 tggaagtgct ggaccttgtt ggtttgaaac acaaagttcg agtctttcct agcgaattat
    10021 ccggtgggga acagcaacgt gtctctattg ctcgggcaat agtgaatacg cctaaagtac
    10081 ttatcgcgga tgaaccgaca ggaaatctgg atccagaaaa ctcatgggaa atcatgaaat
    10141 tattagatcg gatcaatgcc caaggaacta ctgtcgtgat ggctacacat aacagtacga
    10201 tcgtaaatac gattcggcac cgagtaattg ctatcgaaaa cggccgcatt attcgagatc
    10261 aagcggaagg ggaatatgga tacgatgatt agaacctttt tccgccattt attagaaagt
    10321 atcaagagtt tgaaacgtaa cggctggatg acagttgctt cagtcagtgc ggtaacgatc
    10381 acattgactt tagtcggtgt attcatggca gttatcatga acgcaactaa attagctcaa
    10441 gatatagaag gaaacgttga tgtctctgta ttcgtagata tcggtacaaa agacgaagag
    10501 atgaaaaagc tagaaaaaga attgaatgac ttagatcatg ttaaaaaagt tgaattttca
    10561 agcaaagaac aggaattgaa aaaaatccaa aacgaaatgg gtgattcctg gaaattgttt
    10621 gaaggtgaca ataacccgtt atatgatgtg tatgtagtaa gtgctactga tccttcttat
    10681 acaaaacaag tagcagaaga agcagcagat ttgaagaatg tttctcgttc tgattacggt
    10741 ggagcttcct cagatcggat tttccaaatc gctggtgcag ttcggacatg gggattagtt
    10801 gcagcggcat tgctgctgtt cgtagcgatg tttctcattt cgaacacgat tcggatcacg
    10861 attttgtcta gacagcgtga aattcaaatc atgcgtttag tcggtgcaaa aaatggatat
    10921 attcgctggc cgtttttcct tgaaggtggc tggatcggtc tgatcggtgc gattcttccg
    10981 attttgatca tcacatttgg ttatgcatgg tttttccaat tgatcaatcc atcattatta
    11041 cgttcccatt attcattgat tcatccacaa aatatcgttt ggaaaatcaa tcttttaatg
    11101 gtcggcattg gtgtgatcat tggttcactt ggctcgatca tttctatgag acgtttcttg
    11161 aaattctaat accacaatat aaataaaatg agaaagttgg acagtttcgt tccagctttc
    11221 tttttttatt ggctaggaga atagaaggaa gcggcttttt tccctttact ttaaaaaaca
    11281 acttgggtat tcttaagttg gtagaagaag aggtgaagcc gtgaaaatta tcgttgacaa
    11341 tttttttacg atccttttga ttatgaatat tttgctttcc tttatcatcg tttttcgaga
    11401 aagaaaggaa actgctcaaa catgggcatg ggtgctcgtt ttgatgttca ttccagttgt
    11461 aggatttatt ttatatattt ttttaggaag agggatttcc aaagagaaaa tctttgattt
    11521 gaagatccaa gggaaaatcg gtaagaacct ggaaattgaa gaagacaaac aggcattgat
    11581 gcgagggctt tatcctcatc cgcctactgg acaagtagat gtgaaacaat tgatctatat
    11641 gctcacggta tttgaaagta cgctctatac gactaaaaat gaaatcactt tatttacaga
    11701 tggtcgtgaa aaattcgatg cgctgattga agatatcaag caggcagaag accatatcca
    11761 cttccagtat tatatttatc gcagtgatgc attaggtgaa gaagttcgag atgcattgac
    11821 agaggcggca agacgagggg taaaagtcag agtccttctt gatgcatggg gttccacaca
    11881 agtttcttcc agcttttttc agaatttgaa aaaagcaggc ggagaagtcg catttttctt
    11941 tcctctattt gttccgtata ttaatcctcg gatcaattat cgcaatcatc gtaaaatcgt
    12001 agttattgat gggaaaatcg gatatacagg tgggttcaat gttggaaatg aatatctagg
    12061 attggtaaag aaatttggtt attggcgaga caatcatttg cgtatttacg gagaagcggt
    12121 gtataccttg caaaaccgtt ttttgatgga ctggaattct cagcatacaa aagaagtagg
    12181 ctattctccc aaattttttc cgtccattca ctctactggt gatatagctg ttcaaatcgt
    12241 gacaagcgga ccagataccg aacatgaaca aatcaaaatg acttatttga aaatgatttc
    12301 gttagccaaa cgggagatat tgatccaaac gccttattac ataccggatg gatcgattca
    12361 tgaagcactg aagttggctc ttctttcagg cgtaaaagtt catatccaaa tacctaacaa
    12421 accagatcat cttctcgttt actgggcaac gtattctttc gctgcagagc taatcgagta
    12481 tggagcaagg atcgaaacat atgagaatgg atttattcat gcaaagacga tgattatcga
    12541 tggtggcatt gtttcagttg gctcagcaaa tatcgatgtt cgttcatttc gcttggactt
    12601 tgaggtcaat acactggttt atgatgaacg aatggcttca cgtgtaagaa aagctttttt
    12661 tgaagactcg aaaatttcta cgcatctcac aaaagaaatg tatgaaaacc gaggaataat
    12721 catcaagatg aaagaaggat tagcaagatt gatttcgccg ctcctttaaa acataaaaag
    12781 gacatgagaa ccatcaacag atggagatct cgtgtccttt tctaacgggg ccatttctga
    12841 gagtacgtca gaaatggaga aagcgtctcg catgagcgaa attaactttg gggtatctct
    12901 tgttttttgg ggaaagcaag aaataagcta atatatgcta ttaaaagaga cacatttcaa
    12961 agccagtata gcatagaaac ttttaatgag atagtttttt ttacaattat gagaatttat
    13021 tttgttattt aatgattttg attcgttttc atcaagtaat gaaaatgata aaataaacat
    13081 actttaggat taaaggagat ttctaatgaa aaaacttaaa ttattcttac caataatagg
    13141 attagctatt cttttatcag gctgttcaaa gtggattgat cgaggagaat ccattacggc
    13201 tgttggctct tctgctttac agcctttagt agaaacagtt gctgaaactt accagacaga
    13261 aaatcctgga aaattcatca atgtacaagg gggcggaagc ggaactggtt tgtctcaagt
    13321 acagtcagga gcagtcgaca tcggaaactc tgatttgttt gcggaagaaa aatcggggat
    13381 cgatgcctca gcattagtcg atcatcgagt agcagttgtt gggatcactc ctattgtgaa
    13441 caaggatatt ggcgtaaaag acatcagtat ggaaaacctg aaaaaactat ttacaggcga
    13501 gatcaaaaac tggagtgaag tcggtggaaa agatcagcca gtagttatat tgaaccgcgc
    13561 ttcaggaagc ggaacccgta gcacgtttga aaaatgggta ttggacggag aaacagctgt
    13621 tcaggcacaa gaacaagatt caagcggaat ggtgcgccag atcgttgccg acacacccgg
    13681 tgcaatcagc tatgttgctt tttcttatgt ttccgatgat gtagatacgt tgaatgttaa
    13741 cggtgtagaa ccgacagatg aaaatgtgac aacgaacaaa tggacgattt gggcatatga
    13801 gcatatgtac acaagagatc atccaaaaaa actgacaaaa gaatttttag actatatctt
    13861 atctgatgaa gtacaagata acatcgttgg agaactaggt tatatccctg tttctcatat
    13921 gaaagtcgaa cgtgattggc aaggaaatat cgttaaagag taatctttac acaaaattta
    13981 caaacttagc taaactttta catgttatta aaaccaaatt gatgttcaat ttgtacactg
    14041 agtgagtatg ttaaacctga ttaaggggga gtcatctgtg gaagatgtga aaaaagtatt
    14101 attaacaaaa tcaaaacgtt caaaaatgga acagcgtgga aaattcatca gttttttatg
    14161 tatcgcgctg atcgtgttgg ttgtattatc tattttttat tttgtagcta gtaaaggatt
    14221 ggccaccttt tttactgata agatcaatgt atttgatttt ctgtttggta cagattggaa
    14281 tccaagccaa atcggagcaa atgggaaacc aatggtaggg gcattgccta tgatcacagg
    14341 ttcgtttatt gtgacctttt tatctgctat cgttgccacg ccatttgcca ttggtgcagc
    14401 agtattcatg acggagatct ctcctaaaaa cggaacgaag atcttacagc cggtcatcga
    14461 gctgcttgta ggtattcctt ctgtcgttta tggttttatc ggtttatcag ttatcgtgcc
    14521 ttttgtcaga agtatttttg gcgggactgg gtttggtatc ttagctggta catttgtgtt
    14581 gttcgttatg atcttgccga ctgtcacaac gatgactgtt gatgcattaa aggcagtgcc
    14641 acggcactat cgggaagctt cattagcgct tggtgccaca agatggcaaa caatctacaa
    14701 agtcgtgcta cgagcagccg tgcccgggat tttaacagca gtcgtttttg gtatggctcg
    14761 tgcgtttggt gaagccttag ctatccaaat ggtcatcggt aatgcagcat tgatgccaac
    14821 aggtcttact actcctgctt ctacattgac gtccatctta acaatgggaa tcggaaatac
    14881 aatcatggga acagttgaaa ataatgtgtt atggtcatta gcgctgatct tgctattgat
    14941 gtcattattc tttaatatcg tgatccgtat gattgggaag aaaggagagc tgaaataatg
    15001 aacgcaaaac gctcagataa aattgctaca ggtgttttat atatagtttc ggggatcatc
    15061 gtattgattt tagcagcttt actactttat atcttagttc ggggaatacc gcacatttca
    15121 tggtccttct tgaccgaacc ttccaaagcc taccaagaag gcggtggaat cgggatccag
    15181 ctatttaact cgatttattt gcttttgatc acgatgatca tcagttttcc tatttcttta
    15241 ggtgcgggta tctatctttc agaatatgct ggaaaaaact ggctaacaga tgtgattcgc
    15301 acgtcgattg aaatcttgag ctcgttgcct tctgtcgttg tcggtttatt cggtttcttg
    15361 gttttcgtta ttcaattcca gtatggtttc tcgattattt ctggtgcatt ggctttgact
    15421 gtattcaact tgcctctttt gactagaaca gttgaggagt ctttacaagc ggtccattac
    15481 acacaacgag aagcaggatt ggccttaggt ttgtcacgct gggaaacagt gataaaagtg
    15541 gtggttccag aagcgacacc agggattttg actggtgtga tcctaagttc tggaagaatt
    15601 tttggtgaag cagctgcatt gatctatacg gctggacaaa gtgcacctgc gttagatttt
    15661 acgaactgga atccattaag tgtttcaagc ccgatcagca ttttccgtca agctgaaaca
    15721 ttagctgtcc atatttggaa gatcaataca gaaggcacga tgcctgatgg tgtttctgtt
    15781 tcggctggcg cttctgccgt gttgattatt gctgttttac tgttcaactt cggcgctcgt
    15841 gcgattggca aacgattata ccgaaaaatg acttcagcct aaggagaaga agcaaaatga
    15901 aaaaatataa ctggaatgaa acaaatgtca tcaccatgcc aaaagagaaa gaagtagcac
    15961 tatatacgga tgatctacat gtctggtatg gcgacaatga agcaatcaaa ggagtggatc
    16021 tggaatttga aaaaaataag atcactgcac tgattggtcc ttccggctgc ggaaaatcta
    16081 cgtatcttcg ttcgttgaat cgaatgaatg atgggatcgc taatacgaaa atcactggga
    16141 aaatcatgta tcaaggcgta gatattaatg cgcctcaagt ggatgtttat gagatgcgca
    16201 aacggattgg catggttttt caacgtccta atccgttcag caagtcgatt tatgacaata
    16261 ttacatttgc tttgaaacag cacggcgaaa aaaacaaaaa gaaattagat gaaatcgttg
    16321 aaacaagctt gaaacaagct gccttgtggg accaagtaaa agacagcttg gacaaaagtg
    16381 ccttggcttt atctggcggg caacagcaac gtttatgtat tgctcgtgcg atcgcgatga
    16441 aacctgatat cttgctgcta gacgagccgg caagtgcttt ggaccctatt tcgacaggaa
    16501 cggttgaaga aacacttgta aacttgaaag aagactatac gatcatcatc gtgacgcata
    16561 acatgcaaca agcagctcgt atcagcgact atactgcctt tttctattta ggaaaagtaa
    16621 tggaatatga caaaactaac aagatattta ccagaccaaa aattcaggca accgaagatt
    16681 atgtgtccgg tcattttggt taagacggag gcctcaacat gacaaaagag attatttcat
    16741 ctaaagatct tcatctgtat tatggaaaga aagaagcact aaaagggatc gatctaagct
    16801 tcaatcaagg ggaaatcact gcgatgatcg gtccttctgg ttgcggaaaa tccacttatc
    16861 tgcgttcttt gaaccggatg aatgacctga ttccgaatgt aacgatcacc ggaagtgttt
    16921 tatataaaga caaagacatc tatggtccaa aaacagatgt tgttgaacta agaaaagaaa
    16981 tcggcatggt attccagcag ccaaacccat ttcctttttc tatctatgaa aatgtcatct
    17041 atggattgaa attaaaaggg gaaaaagata aaaaagtcct tgatcaagta gttgaagaaa
    17101 gcctgaaggc agcgtctgtt tgggatgatg tgaaagacaa attgcataaa agtgctttat
    17161 ctttatccgg tgggcagcag cagcgtgtat gtatcgcacg tgttttggca gttaatccgg
    17221 aaatcatctt attggatgaa ccaacaagtg cgcttgatcc tgtttctact gggaaaatcg
    17281 aaagtatgct gttagaatta agagagcggt atacgatgat catcgtgacc cataatatgt
    17341 ctcaagcttc acgtatttca gataagacgg cattcttttt agatggtcac ttgatcgaat
    17401 acaatgatac gaagaagata ttcatgaatc cagaaaaaca agagacagaa gattatattt
    17461 caggtcgatt tggataagga ggaaacggaa tgctacgtac acaatttgaa gaagaattgc
    17521 tgaatctcaa taatcagttc tacgaaatgg ggatgatggt gagcagcgcc gtgcacaagt
    17581 cagttcgtgc atacatcaaa cacgataaaa tcttagccca agaagttatc gaaaacgata
    17641 taaatatcaa taacatggaa acacgcttgg agaagaaaag ttttgaaatg atcgcattgc
    17701 aacagccagt tacaactgat ttgcgtatga tcattacggt catgaaagct agctcggatt
    17761 tagaacgtat ggccgatcat gcggtgtctg ttgctaaatc cacgattcgc gtaaaagggc
    17821 agacgagaat accggaaatc gaaaaagaga tctccgatat gtccgattat gtgaaaaaaa
    17881 tggtggacaa cgtcctagtt gcttatgtca aaacagacga aaaagacgca cgtacgatcg
    17941 ccaatatgga tcaaagagtc aatgactatt tcaatcgaat ctataatgca acaatcaaaa
    18001 atatgcagga aaatcctgag acagtcatca gtggaacaga ctacttgaat gttgccagtt
    18061 atctagaacg gatcggagac tatgtgacaa acatctgtga atggatcgtc tacttggcta
    18121 cagggaaaat cactgagtta aatacgaacc acaatgagat tttctaaact gaagctgtga
    18181 ttatgccgtt tgtttaggaa aagaggttgt gaaatcagcg gttgtgaaaa agctgttctg
    18241 catcaagtaa taagaacagt caaacaaaaa atagctcctc atatttttcg tttggctgga
    18301 cttattaccg cagatgcaag cttttgaaca ccgtttattc gggaaagggt ttgtgacaaa
    18361 acatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa aaaaaaatta
    18421 gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag catcaagccg
    18481 ctttatatga agtacctgtc atcgagatag aaaaagtacg aataaaagag gatcatcaat
    18541 tgattacagg aaaactgatg aacacggatc aaaaaggaga atcgatttat ttatcggttc
    18601 cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg tatctagtcc
    18661 aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg cttttcttct
    18721 tcacaagtat cttcattggc gccatgttgc ttattggtgg aaaacagggt ctatttgctt
    18781 taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc tttactaaaa
    18841 cagaagggat ttctctatta aatatatcta tcctgttcgt cgttataggg atgattatta
    18901 gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca gctgcagtta
    18961 cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatcattgg aaagaccaag
    19021 gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt tttctttcta
    19081 gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc attgcgacat
    19141 tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg ctttcaggaa
    19201 ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc gtctatatca
    19261 gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtcgttc aaagaaacga
    19321 tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta ggaatagtat
    19381 tatcgatccc tatctcggtc ctcttttttc tgactgtaag gaggctgaaa cgatgaatgt
    19441 acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg gtacgcaagg
    19501 tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcctgt tgttattcgg
    19561 ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat tgattgctgt
    19621 ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt ttgtgagtat
    19681 actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt tagcaatcca
    19741 aggttttaca tcggtagaac tagaagaatt atctggctta gataagactt tagcgattga
    19801 ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg ttttagatgc
    19861 gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc aattatcttt
    19921 ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt caacagtaat
    19981 gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt ttattgattt
    20041 agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat atacgatggc
    20101 tattcttacc acattgtccg cactacttgt tcttccttta acctgcgtga ctacagcata
    20161 tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac ttaagtccta
    20221 tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc aaacaatttt
    20281 gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag tgaaaaaagg
    20341 aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg aaaaagacga
    20401 aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa aagatcaggc
    20461 aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc cagttgatcc
    20521 aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg aactagcaac
    20581 tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg ctgacaataa
    20641 ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag aagaattagg
    20701 cgagttgaca gaagaagaat tagcacagcg tcagacgtta gaagcagaaa tcaaagaatt
    20761 agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag aattaaaaga
    20821 tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt ttgaattgcc
    20881 agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa aaatgtcaga
    20941 agctggatca cagttaggca aattcttgaa gaaaacattc caaactgttt cagaaacagt
    21001 gaatgacaac atggaatgga aagatgtcag cttacgtgtc cctgggattg cgacaacgaa
    21061 atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata tcaaggcagc
    21121 aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg aagctaagat
    21181 caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac ggagccagat
    21241 tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc gtgcagacct
    21301 tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc taaatggcaa
    21361 catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga atggtaacat
    21421 catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg gcaatatcga
    21481 tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta cagtgacatt
    21541 tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat ggtgatgtac gtcttacgat
    21601 taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg tcaaagtcgc
    21661 tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa gtatcaacag
    21721 ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc aaatgttgca
    21781 attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa ctttcaacga caacaggaag
    21841 tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc agccttgagc
    21901 aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc cttattgccg
    21961 atagtctatc tcttgaacac cgtttaccaa gagattatga acctgccaat tatcgccgag
    22021 tcacaaggaa taatttcaga aaaagctcgt cacgttttat gaatatggta atttgtgacc
    22081 gagaattttg cagaaagaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22141 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc taataaaaat
    22201 gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga tccgactgtt
    22261 atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat tattttatac
    22321 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22381 aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac aaaaaaacgc
    22441 aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg tactttcaac
    22501 gtattttagt taatacgtta acattcatat cattggctgt tctttttcct gcgatggtcc
    22561 atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg ttgaatatgt
    22621 ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca ttgggattgt
    22681 ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg ggagcagaac
    22741 attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg tcgcttgtca
    22801 atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg aactatcggc
    22861 gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat taaataaaaa
    22921 cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag agaacactct
    22981 aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt agtgaaaaaa
    23041 ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat cgtaactggg
    23101 gacatctcac gcccaggact agagttgact ggttatttta attattattc ccataatcgt
    23161 ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc agaagagcgt
    23221 ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat ttcaagagga
    23281 ttaccagctc cagaagaaat gatccaggca gcaaatgaaa atgggatagc tgtactgcgt
    23341 tctcccatct caacttctcg tttattagga gaattatcca gttatttaga tggtcgcctt
    23401 gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg tgtactgatt
    23461 caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa acgcggccat
    23521 cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac ggtgatcgga
    23581 gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat cattgatgta
    23641 atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt ggctgtttat
    23701 ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga cacgaccgtt
    23761 gaaatcgcgg aagtgggcat tccacaaata aaaatccctg tcaaaacagg acgaaacgtt
    23821 gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac gtacaatggg ctttgacgca
    23881 acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg caacgactag
    23941 caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt gtagggattc
    24001 ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgctatg tggcttagca
    24061 gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt atgttgtggg
    24121 gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat cttccccagt
    24181 atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct atttatggcg
    24241 gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt atttctttgt
    24301 ggactttttt agatatcgct gcaccaagtg tattattagc acaggcaatc ggtcgttggg
    24361 gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc ttagaaagtc
    24421 tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat cgtcaaccaa
    24481 catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg tttttacgaa
    24541 agaaaccaca ccttttgaaa aaaggcgaag tccttttatg ttatctaatg tggtatagtt
    24601 tcgggcgttt ctttattgaa ggattacgta cagacagtct ttacttttta ggtgtgatcc
    24661 gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta tttatttggc
    24721 gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat caacaaatta
    24781 tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat gaattataga
    24841 gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct cgccgaaaac
    24901 ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat caacacccaa
    24961 cacacgaatc aacgttattt accagatcat tatctacccg attccatcca agcattcacc
    25021 aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc aacgaaagca
    25081 atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt gatcatccat
    25141 gcaagtaaag gattagaaca gggaacacat aaaaggatct ctgacatctt gttagaggaa
    25201 atcccagcag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca tgcagaagaa
    25261 gtagcaaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa agcagcaaaa
    25321 tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc tgatgtgatc
    25381 ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg cgctattcac
    25441 ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt agctgagatc
    25501 agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt aagcggaata
    25561 ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc aggcaacttg
    25621 ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt cgttgaaggt
    25681 gtgaatacta cccaagcagc gatggaacta gcccaaatga tggaaatcga aatgcccatc
    25741 actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc cgcaaaagaa
    25801 atcatgttac gtgatggaaa aaaagaaaat gaattctcat taacaatttt ttgaaggagc
    25861 gaatgtatat gaaagttaaa aaagcagtta ttccagcagc aggattagga acaagatttt
    25921 taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa ccaacaatcc
    25981 agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc gttacaggaa
    26041 aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa acaaacctaa
    26101 gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat gtcaacttgc
    26161 atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa gcaagagcct
    26221 ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa gacgaagtac
    26281 cattgacaca gcaattaatc gatgattacg aacgtactca tgcttctacg atcgccgtta
    26341 tgcaagttcc tcatgatgaa acttcaaaat acggaattat caatccagga gaagtattgg
    26401 aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc aaagcaccaa
    26461 gtgatttagc tattattgga cgttacttat tgactccaga aatttttgat gtattgtccg
    26521 agcaacaacc aggagccgga aatgaaatcc aactaactga cgcaatcgat acattgaata
    26581 aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttacgatgtc ggtgacaaat
    26641 ttggttttat gaaaacaagt attgaatatg gtctaacaca tccagaagta ggtgcacctt
    26701 tacgtgagta catcctgtct ttaggtaaaa cattagaagc agaaggaagc aagatcgatc
    26761 ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataaccc ttttttctaa
    26821 cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt tgtttaaaaa
    26881 acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat catgtcagga
    26941 ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt ttttatcgtt
    27001 cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag atcagactgt agcagaagca
    27061 aatacgacga tcgaaatcgt cacaaaagac gtagatcttt tgtctagaca agtagaaggc
    27121 ttactggtca aaagtaatga actgctcttg gatatcaatg gaaaagtcgc aacgatcgat
    27181 ccattgttca cagcagttgc tgatttaagt gaaagtgtat cagaacttaa tcattctagt
    27241 aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga cagctaaagc gacggttgcc
    27301 ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca aaaaacatac tgaaacgaca
    27361 ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt aggtgcagta gtcggaggca
    27421 cagccgcagc aatcgccgcg ttattgttag caccaaaatc tggtaaagaa cttcgggatg
    27481 aattagctgc ccaagcggat gatttgatgg atatggcttc agattacaca gatatggcga
    27541 aagaaaaatc gactgagtta acaggtattg caaaagaaaa agcgcaagat ctttctcaac
    27601 aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag tgaatcagca gatgaagcag
    27661 gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca atcagctgac ttatctaatc
    27721 gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga tgaattagga agtattgcta
    27781 aagatacttc agaagatatc atgattgatg taaaagacgc tgctggtaaa gcaggagata
    27841 ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga tgactcaaaa gaaatgatga
    27901 aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat aaaagataca atgacagatt
    27961 catctacaaa tcactaaaat cacaaattta tagaataaaa aggtgggagc ggactagctc
    28021 tcaccttttt tactcttatt tttctaaaac aagtaattct tttggtgtag tcgtgaatgg
    28081 cacacagcca tcgcttgtca catatacaca gtcttcgata cgtaccccta ctttttcagg
    28141 aatatagatt cctggttcaa gagagaagca catcccttct tcgattacta agtcatttcc
    28201 ttctacaagg gaagggaatt cgtggacggt tgttccgatc ccgtgaccta aacggtgatt
    28261 gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat caagttcacc
    28321 tgctgtcaca cctggacgga cggcttcttg tgcggcaagc tgtgcttcaa gagtgatgtt
    28381 gtagattttt tcttggaagt cggttggttt ttgataagca attgtacgag tagcatcact
    28441 gcaatagcca ttccaaacaa cgccaagatc gaataacaca agttcattcg gtgaaatctt
    28501 cgtatttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga ctaatgtgtc
    28561 gaaagacatt tgtgaaacac cttgtttttt caattggtat tcaatttctg caatgatcgc
    28621 ttgttcagtc acgccttctt tcactgcttt aaagccaatc tcaaatgcaa catcggccca
    28681 tttaccagct tcgatcagtt tgtctttttc ctctagagtt ttgatcagtt gtagacgttg
    28741 gatgatggaa gttagatcag tgtaagaatc cgttcccgtc aagatatcag agatagcatc
    28801 gaagcgctcg acagtcaagg cgttcttttc aatccctaca ttgccaggct gaccatagcg
    28861 agcacgaagt tcttttccga tgatctccca agggttttcg ctgtctaaat agccacggac
    28921 atcatacgcc caactactgt tttgagcatc ttcaacttcc aaagcaggag tgaaaaggaa
    28981 aggatcttgt tcaagaggga taaaaagagc tagtacacgc tcatgaggat cactttcgta
    29041 tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcga cttggtgttg
    29101 tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac ctactttcta
    29161 aattaatttt gtattcaata attgtaccac tgatttatta aaagaccaac gttctaaaaa
    29221 ctgaaaaaag taaaaatttt catgaaaaca gagataaaac ggttgcaatt cttgagatta
    29281 tttgatattc tattttagaa atgtaagacg tattttcaga aaacatataa aatatgaata
    29341 ggagaacaca aatggaaaag caaacaatca ctatctatga tgtagcgaga gaggcaaatg
    29401 tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc aaacgttaaa ccagcaacaa
    29461 gaaaaaaagt cttggaagtt atcgatcgct tggattatcg tccaaacgca gttgcgcgtg
    29521 gcttggcaag taagaaaaca acaacagttg gtgtaatcat ccctgatgtc agcaatatgt
    29581 tctttgcttc tttagcacgt ggtatcgatg atgtggcaac aatgtacaaa tacaatatta
    29641 tcttagcaaa ttcagatggc aacgaccaaa aagaagtcaa tgtattgaat aacttactgg
    29701 ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat cacagacgaa atccgtggag
    29761 aattttcacg ttcaaaaact cctgttgtat tagctggctc tattgatcca gatgaacaag
    29821 taggtagcgt aaacattgat tatgtatccg caacaaaaga tgcagtaaac aaattggcaa
    29881 aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt gatcgatccc atcaatggac
    29941 aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa taacctacca tatagcgaag
    30001 gattgatctt tgaagcacaa tacaacttca aagatggtct atctctagtc gatcggatcc
    30061 ataatagcgg tgcaacagct gtctatgttt cagatgatga attagccatc ggtattttgg
    30121 atggtttatt agaccgtggc gtgaaagtac cggaagaatt cgaaatcatc acaagtaaca
    30181 attcactact gacagaagta gctcgtcctc gtttaacaag tatcacacaa ccattatacg
    30241 atttaggggc ggtatctatg cgcctattga cgaaaatgat gaataaagaa gaagtcgaag
    30301 aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg atctacaaaa taataaaagg
    30361 acaaccattc agaggttgtg tgcctgccgt ttaactctgc gttacaagga gcaatttcaa
    30421 aaaacagcat ctcatgtttg atgaatattg tgactcgtga ttcggagttg gcaggagaac
    30481 accgtttatt tgggaaaagg ggttatgaca aaattttgtt ataatccttt ttttgtgtct
    30541 taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat ccgtgtatag
    30601 atgctgcatt ttgttcacca ccatcaaaaa aataagggac tgtgacaata ctattgtcac
    30661 aatcccttat tactgtgtat tctattaaat caatcatctt tctcatctga ttctttagtt
    30721 gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata gttttcatcg
    30781 gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc ccatagcgat
    30841 tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt tccaggtgtt
    30901 tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc gaatttctca
    30961 tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt ggcaagataa
    31021 ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg ccctgcccag
    31081 ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta ttcattggta
    31141 gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct tgagatggca
    31201 tctttgaatg gtgtagtgat ttttgcattg atcacactac gcatcatgtc attcatgatc
    31261 gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata aattacattt
    31321 cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc gccgtttgct
    31381 aaagcctgaa atccgttggt ctgttgaagc gtcgtgacat ctactgtacc taaaggagca
    31441 gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta gtgctcgtaa
    31501 gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata agccgggata
    31561 ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc ttcgttagta
    31621 gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc ataatctgat
    31681 acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac aagaactggt
    31741 ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg attctgactg
    31801 taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat caatacattt
    31861 ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt ttcaacgcca
    31921 ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata tcctttattt
    31981 tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc agacatatca
    32041 gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt catcacagta
    32101 taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa gtcttttgta
    32161 aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc acgatacata
    32221 ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt tagcgcgcct
    32281 gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat ataagcagct
    32341 tgcggcagcg tcacatcttt tgcatctaca ccaaagattc cttttgcagc ttcctggact
    32401 ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa atacgtagca
    32461 atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat ctcgtttgct
    32521 ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa
    32581 gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg taaaacagct
    32641 ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat aattgcagtt
    32701 ttcaataaag gtgaaatctt atcagatgaa atagtcgtgc gcatcagatc tgtctggatt
    32761 tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat gtttcctagt
    32821 tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag
    32881 ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt aataaaaaca
    32941 gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc tttttttctt
    33001 ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac taatggtttt
    33061 atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt tttggcagga
    33121 atttcttttt taaaagagtt aaaacgtgag aaaatgatct ttattataaa agagttctta
    33181 agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa aaatcaatca
    33241 ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt gcctttcaag
    33301 gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga aaatgctgtt
    33361 aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct atacaggcgc
    33421 ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg gaaaagcgta
    33481 catggcagaa aaaggtctaa caaatatcgt cgttcatgcc ccttatatca ttaatttggg
    33541 gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag aagagattgt
    33601 tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc atgtaggggc
    33661 aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt tgacaaaaga
    33721 acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac
    33781 atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat tatccgttac
    33841 ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact tttcaggtgt
    33901 aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc atatcaatga
    33961 ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt ttggaacaat
    34021 cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata tttcgaaaat
    34081 cttggaaact ccctatgtag gcaaagataa aaagaaagct tacgcaccat atggaaaaga
    34141 aatcgccatg ttcaaagcgc aaacattcga tccagaagtg tttcctgagt tattagcaga
    34201 agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag gcacattgcc
    34261 ttgtcctttt ttgtttaaga aacaaaggag tactttttgc agtctttttc atcatgcatc
    34321 ttctcccccc tctttttaaa tttagacaaa caaggtatac tggtagaaaa gtaaaaaaac
    34381 gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat gctgaacact
    34441 ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct tggcatagcc
    34501 ctattattta caattattcg tttgtattta tatagtaaag caatctatgg aacagacatg
    34561 tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt ggacggtaaa
    34621 tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag cttattttta
    34681 gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt cagtattttt
    34741 gccagtatat tggttgcact cgcgtttaaa ccgattgtga agaacaagta tcttctttta
    34801 agtatctatt tttatttttt gttttctccg attagtttta cacatgaata ttccactcga
    34861 acatatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg tttattagct
    34921 atttattacc gcaaatacgg ttctagcaga aaattgctgc catggttttt agggctttct
    34981 gtcgcttttc ctttcttttg gttcattcgc gaagattcta tctggcttct tccttttact
    35041 gtagtagctt tgggtatcag tttcgtccag atcttgctag ggcaagactt ttctttcaaa
    35101 gaagtaaaga agttaaaaaa acgactcttt cttctctcga aacgggaatg gctgaaactt
    35161 tcattgtttt tacttcctat agttggcttt tccatcgtct atcaatggat cgaatataaa
    35221 aatgagacaa catacggtat tgcaaccatc aatgaccgtt ccagtggaga atttggacgt
    35281 ttgatgaaac aactgatccg aatagatgat gaaacgaact tgaataaaga aaacagtgag
    35341 atatgggtct cacatgctgc actaaataaa gcattagccg catctcctac attccatact
    35401 gtttctaccc gtattgacga attatatacg actcacgcgt ggactcaagg tggaaaagta
    35461 aaagaattac ccggagatat catcttttgg gcattgcgag atgtggtaag cgaatctggt
    35521 tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa ccaagaatta
    35581 gaagaagcct atcacaaggg aacaatcaaa aagaaaaaag agttctatct gatgggatca
    35641 ggagatggaa agacgatgag tgatctgcca atcgtattcg atttccttca atcaggatac
    35701 gcttatgcat ggcgatataa agatttccat caaggaggaa attatagttt cggaacgcaa
    35761 gaacaagtca aacaggctca agaattatta aaccttccgc taatggagaa ttggatcgat
    35821 agtaatgaac cgagaacacc taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc
    35881 atcataaaaa tttaccaagt tctggctatt cccgtgctga ttgcttcaat agcagggata
    35941 ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta tagttcctct
    36001 ttgattgtgg tcaccggatt aatactgaca caattggttt ttcttttcgg tgtctcatgg
    36061 ttttgttctt atgcgccaga aaaaaaggac tattttttaa gtgtctacac tggtgcaggc
    36121 gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa attaaatctg
    36181 aaaaaagctt tatcttctaa ataaaaagac ccttggtagc tagattttta acctaactat
    36241 caagggtctt tgtgtggaaa aatcagtttt tattcgattt cttctaccgt ttcctgcaaa
    36301 agctgttcat attttttgtt tcctttatat aaatcaccaa tcgtccatgc caacaggaga
    36361 aggacaagaa caatgattac ataagcgatg tgatcagcca atgctagttg agcggaagtg
    36421 gcttgctcgc cgaagaaact ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt
    36481 agactaatca cagagaacca gccaagaagt tttacgatga atgaattttt aaatcggcta
    36541 cccatctccg cttggctgtt ggtcatcatc agtaaaggaa tcatcgaaaa cggcaacgca
    36601 aaagccaaga acacctggga ttcattcatt aagttattca aagcggcatg ttcttcgatc
    36661 gttccttttg tacttgtaaa caaaacacaa atcaatacag gaatcacaga aagcaatcgt
    36721 gtgaccagtc ttcttagcca aatcgggata cgcatatgga tgaatccttc catgatgact
    36781 tgaccggtca acgtaccagt aatagtagag ttctggccag aagcaagaag tgctacagca
    36841 aataaagtgg acaagatacc agattttgct acagcaatca aaataccatt gctcatagct
    36901 gatggatcag ataaagcttc atacagacca aaaaaggaag gatcttggac agaaccggtt
    36961 ttgaagacag cgacacccat gatcaatagc agtgcattga ctagaaaagc cattgacaac
    37021 tggatattag aatcccaagt agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg
    37081 tgatcgatct tccgagtttg tgaaactgct gaatgaagat ataagttatg cggcatgact
    37141 gttgcaccaa taatccctaa agctccagcc agcggagttt cgccgccgat gaccggtgag
    37201 tctgagaatg tatgactgtt agggataaag ccagcaaata aactgtgcca atctgggtta
    37261 gacaagccaa cttgatagac aaaaacgaat aaaatgacga aaattaagca gacaaccagt
    37321 gcttcgatct tacgaaaccc gacttttgtc agtagtaaga gaacgaaaac atctaataca
    37381 gtaataaaga cggcaatcac caatggaata tcaaaaagta agtagagggc gattgctgca
    37441 ccaatgactt ctgcaatatc tgttgccata atggctaatt cagtcaagat ccataaaatg
    37501 acacctaatg tcttacttgt gcgtgctcgg attgcctgtg ccaaatccat ttggctcacg
    37561 attcctaatt ttgctgccat atactgtaaa agcatagcaa tcagactaga catcaaaata
    37621 actgacatca ataaatattg gaaattttgt cctccggtga tcgaagtaga ccagttaccc
    37681 gggtccatat acccaactgc tactaacgca ccaggtccag agtaagcaag aagtgtttta
    37741 aaaaaacttt tcccctttgg tacatcaact gttccattta cttcagctaa agataaacca
    37801 tttgtatgtt caataagtcg atgcttatga aattgtttat tggattgatt attcaagaaa
    37861 aaaccacctt tcctatttat ctacgattac attctacacc tcaaaaagaa aaaattaaag
    37921 aaaaagattc ggtacgccta attttttttc atggcgcgtc ttgctagatg acgaaagaaa
    37981 ttttgttctc ctctaaaaaa accactattt tttgcatgaa actaaagaat acgcctttct
    38041 tttttcaata aactttagta aaataagtca ttgagagcaa ttcggaaata gaaaggagag
    38101 atttatatgt tatcaacagg tattgtcgta cttattgcta ttattgcttt gctaatcggc
    38161 gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga aaaaaatcct
    38221 cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa accatctgag
    38281 aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc
    38341 caacgacttt ttacgggctg attttttcag tccgtttttt atttcgtaat ttgttagact
    38401 attcagatta ttcagcaaaa aagacaagca cagacaatcc taggctcttg ataaagacaa
    38461 aggaggagta aaatgtcgat ttttagaaaa ttaggctggt tttttcgtca ggaaaagaaa
    38521 cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt agttcctcca
    38581 aaagtgattg ggatcatcat tgatgagatc gccgataaaa atttatcaat aaagatcatt
    38641 ttcgggtgga tcttcctatt gattatcgct gcttttttac aatatctttt tcgttatatc
    38701 tggcggacga atatttgggg aagtgcagct cgattggaga aagaacttcg gcagcagtta
    38761 ttcaaacatt ttacagaaat ggacaatctc ttttaccaaa aatatcggac tggggatttg
    38821 atggctcacg caacgaatga tttgaatgcg atccagaatg tagcaggagc tggtatcttg
    38881 acctttgcag attcagtgat tacgggcgga acaacgatca ttgcgatgat tttgtttatt
    38941 gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt cacttcacga
    39001 gtgctggggt caaaactcca tgatgctttt cgtgattcgc aagaagcttt ttctagtatc
    39061 aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt tggacaagaa
    39121 aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa aaacaaacga
    39181 gtcaattttt tagactcctt atttgatcca ttcattacct tgattatcgg tctgtcttat
    39241 gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc gatcgggcag
    39301 atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc gattggtcga
    39361 ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga acttttgcat
    39421 gaaaagacgc atatcgtgga acaactgggt gcagtgcaga ccccggcaaa aggcgagctg
    39481 aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt agaacaaatc
    39541 aagtttgcga tccaagaagg agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa
    39601 acgacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa aatccaattt
    39661 ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat cggctatgtg
    39721 cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt ttctaatcca
    39781 aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa caatgaaatc
    39841 aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc tttatctggc
    39901 ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga attattaata
    39961 ttagatgatg cactctctgc agtagatgcc aaaacagaag aagctatctt gcataattta
    40021 aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag tagcgtgatg
    40081 catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg aacccatcat
    40141 gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca attagaagcg
    40201 aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg acaaaatcga
    40261 ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc aaacctttta
    40321 gaagaacgtt catcgttgct atcttatttg cgtttgcttt gtctgtgatc aatgtcctat
    40381 tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg gctacgacac
    40441 aagttatttt gttttttgct ggtatgtact tatttggagt catagtaaaa agtatcattt
    40501 ggtttttcca atggtatttg tattccatgg cctcattgaa gacctatcag tatattaggg
    40561 taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag acaccggcag
    40621 gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt tggtacgtct
    40681 ttttgatggt attgactggg atctttgcag tcgtttcttc attccttgcc atgtttcaaa
    40741 tcaatggaga aattgccttg tggactttgg tgtttttgcc gattttagga atcgtcatct
    40801 ggtattacca gaaattcagt tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc
    40861 tgaacacgaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag tttcgccaag
    40921 aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac acgcgttatg
    40981 cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta tatgcattgg
    41041 cgattgcgtt ggtgttgact ttgtttggga tcgatgcgtt gcattctccc gtagaagttg
    41101 gactgatcta tgcatttacg acctatgtcc agggattttt taatccaatg tctcaaatga
    41161 tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc ttgaagatta
    41221 tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg attacttcag
    41281 gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat gtattaaaca
    41341 atatctcttt tgttgctaat ccgggagaga ccgttgcatt agtgggacac accggaagcg
    41401 gaaaaagctc gatcatcaac gtattgatgc ggttttacga attttatgaa ggtcagatat
    41461 tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag aaaatggggc
    41521 tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt cggatgatga
    41581 atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa gcaaacaaat
    41641 tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt gccagctatt
    41701 ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat cctaaaattc
    41761 tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg atccaagaag
    41821 gattggcaaa aatgcgtcaa ggacggacaa cgattgcgat tgcccatcgg ctgtcaacga
    41881 ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa cgcgggaatc
    41941 atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg caaagcagtg
    42001 gtatataaaa gatgttgtga cagtagaatt ttgacctaag cactagagtg aaaataaaac
    42061 atagcttctc atatttttca tctgtgagag ttagtgtcgt agtcaccaga tttaaaaaaa
    42121 gattcgacag ctagtcagaa gtgcctaata gccactaaga aagcgttgac aaaagcattt
    42181 gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa
    42241 acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt ttgttttgga
    42301 tacagcccta tacatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac
    42361 aacgtcgtaa taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg
    42421 gcattcaaaa agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag
    42481 ttgacaaatg acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag
    42541 tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg
    42601 aatgattctg tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa
    42661 atgggaatat ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag
    42721 tatgccatcg ggaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca
    42781 gaagatgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccag
    42841 caagacaata cctacctgtt tactataaca atacaggata ttctaaatat cgtccgctat
    42901 tactataaga ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta
    42961 cgtttttttc tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag
    43021 gaattgtttc aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata
    43081 gcagactttt tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta
    43141 acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaaaaaa ggaggataaa
    43201 ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct tccactaata
    43261 aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc gtagaaaatg
    43321 tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt cgtttcaaat
    43381 taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt attgtaacgg
    43441 taatgcgagc gggagggcag tatcaagttg tcattgggaa tcatgtgcca gtcgtgtttg
    43501 aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca gaaaaaatga
    43561 gaccgtttga tatgcttatt gatggaatat cgggctgttt ccagccattc cttgctatcc
    43621 tagctgctgg cgggatgatt cgtggactga cagcattcct tgtcttctta ggcgcgtttg
    43681 accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta ttccaattta
    43741 tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa ttcgtaggga
    43801 tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg agtggtgcag
    43861 cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct atttatcaaa
    43921 cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt cccattatct
    43981 ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta cctgagatga
    44041 tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta ggttttttac
    44101 tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt gaaacacttt
    44161 tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa attctggtga
    44221 tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct gtgaatggtt
    44281 ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca gcacttaccg
    44341 cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca gccattgttt
    44401 ctggtattgt ggggattaca gagccagcta tttatgggtt tacattacca cgtaaaaaaa
    44461 tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg ttaatgaatt
    44521 taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat attagaccag
    44581 atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg atggtggtat
    44641 cctttacgct gacttttttc ttttggaagg atgaagcagg tgaaacagat gatatacaga
    44701 aaaaatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct ccactaaatg
    44761 cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg atctaccctg
    44821 aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gaccctgttc ccgacgaaac
    44881 aggcaattgg aatggtttct gaaacagggc tggaattgct gattcatgtc ggattggata
    44941 ctgtgcagtt ggaagggaaa tattttgaaa gcttagtgca gcaaggataa agagtgaaac
    45001 aaggcgatct gttgctacgc ttcgacaaaa agaaaatcga aaaggaagga tacagtttag
    45061 aaacacctgt gatcgtcacc aattacacgg attatttgga tattttggaa aaccaagggg
    45121 agacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt aggtgaagaa
    45181 tggactaggc ttaactattc tttttattcc acttctttat tttcaaaatt gaaaggctct
    45241 ttcatgatgg ctgagctgat agggcgttta tcgaattttt gaccgacaga aattagatgg
    45301 actaaattct gttgataatt ttctgcaaaa acacaactat ataacacgtc tagaagataa
    45361 caaattgatg tattgatcgt aaaattacca atttttgaat acagtcgctc gcgtgtcgta
    45421 agtcgcaaag cggctgtact cattgcagat agcgttgaat caccgatact ggtcaaagct
    45481 aaaattggaa catgattttg tttcaatatt tctgcggtac gaaggatcac attgttttca
    45541 cccgtataag aaatcagaat cccgcacata tcactcgtgc aattgtacgc ttcatagaat
    45601 gattcaccaa aagtggtgac aatcgtgaca tccattttga tgcgcttcat tttcagcatg
    45661 aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt ttttgcttga
    45721 agcattagtt gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag tgtatcatga
    45781 atcgtcatct gttctaaaga agccatttta tttgcgatcg tcataatacc gtctgtggtg
    45841 gaaaaaggaa gattcgcatc tacgtcttga aaattccctt caagataggc tgtttcagct
    45901 aaaaaagcat tacgaaaact gacccagcca tcaaaaccca ttttttttgc tacacggatg
    45961 agtgtagagg ggtggacata acatcgctca gcaatcgctt ggatggtcat ttcgccaagt
    46021 gctggagaat cgcttaagag aaactggatc acttcatttt ctgaaggaga aaaggagatt
    46081 tctttgattt tttcacgtaa taacatgtag ataccttctt cttatttttt tacgtaaatt
    46141 ataacttttt tgaaaatgat ttgtaaacgg atttatacta tataacttaa aatagacatt
    46201 ttttgacaag atatttacaa aaatccacta gaaaacagta aattttgtaa atgttactgg
    46261 aaagaaacag atttctattg tttgttttgt ttacaaaaaa gataatgaaa gtgtaaaaaa
    46321 gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg gaggcagcag
    46381 ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac cgatacgaga
    46441 aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtta gtcagatgtc
    46501 tcagcgtatg tgggatgctt ctccgtatga agtcaagatc tatgcaactc tggatcggga
    46561 agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc aattagatgc
    46621 acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag aaacaaatgg
    46681 tgctggcggg atattcaaag cttttcgtac cgttccagtc gttttggcaa tcgttgagga
    46741 catgaagcgt ttatgtccaa atgcttggct gatcaatttc gcgaacccaa gtgggatggt
    46801 gacagaagca gttatcagat atggcaaatg ggataaagtg attggattat gcaatgtgcc
    46861 agtgatggcg atgatgacag aaccagcaat gcttggcaag aaaaaagaag agctgatcta
    46921 caagtttgcc ggtctaaatc atttccattg gcacaaagta gcagataata aaggaaatga
    46981 ccgcacggca gaattgattg atcgtttata cgatgaagag aatggattac cgaaaaatat
    47041 ctttgatatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc cttgtggcta
    47101 ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag aatatcaaac
    47161 gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctatttg aattatacaa
    47221 agatccagaa ttagaccata aacctgaaca attgcaaaaa cgaggtggcg cttattattc
    47281 agacgctgcc tgtgaaacga ttgcttcaat ctatggaaat aaagaaacac agatcgtggt
    47341 atctacaaag aatgatggag ctgttcctga cctgccggca gattgtgtag tagaagtcac
    47401 tgcctatgtt ggcgcacaag gagcgagaaa tgtagcgttt ggttctcttc ctgctgctga
    47461 aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag cagctgtgac
    47521 tggtgattat gggacagctt tacaggcgtt tacgatcaat ccacttatac gatcaggaaa
    47581 gacagcaaaa cggatcatgg atgagttgtt tattgcacac cagcagtatt taccgcaatt
    47641 caaggagaca atcagccgat taaaagaaga aggaatcgaa gttcaagatg aactggcaag
    47701 agaattatca ggtaatacac atataatgga ggaagtataa tgggattcag aaaagatttt
    47761 ttatggggcg gtgctacagc agccaatcaa tgtgagggcg gttatgatga aggtggacgc
    47821 ggactagcaa acgtagatgt tgtgccaatc ggaaaagacc gttttcctgt tatcaccgga
    47881 aagaaaaaaa tgtttgaatt tgatgaggat catttttatc cagcaaaaat tgcaatcgat
    47941 atgtatcatc gttataaaga agatattgct ttgtttgcag aaatggggtt caagacttat
    48001 cgaatgtcca tcgcctggag tcgtattttt ccaatggggg atgagaaaga gccgaatgaa
    48061 gctggcttga aattttatga ggatgccttt aaagaatgcc aaaagcacgg aattgagcca
    48121 cttgtgacga ttacgcattt tgattgtcca atgcatttga tcaaagaata tggtgcgtgg
    48181 cgttccagag aactcgttgg tttttatgaa aatctgtgtc gagtcatctt caatcgctat
    48241 aaaggacttg tcaaatattg gctgacattc aatgagatca atatgatcct tcatgcacca
    48301 ttcatgggcg cagggctgta ttttgaaaaa ggagaaaatg aagaacaaat caaatatcaa
    48361 gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agattgctca tgaagtagat
    48421 ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata cacttgcaat
    48481 cctgaagatg tttgggcttc tagaaaatca gatcgagaaa attatttctt cattgatgtc
    48541 cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggagcgaaa tggcattcag
    48601 atcgaaatgg aaccagggga tgccgcattg ctgaaagaac atactgttga ttttatctct
    48661 ttttcttatt atgcttcccg agtttcgact accgacaacg aactcttgga acagactgca
    48721 ggaaatatct ttgcttctgt caaaaatcca tatctgcaag caagtgaatg ggggtggcaa
    48781 attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta tcaaaaacca
    48841 ttgtttattg tagaaaacgg cttaggtgca gtcgatacgc cagatgaaaa tggttatgtg
    48901 gaagatgatt accgaatcga ttacctggca gcgcatatcc aggcgatgaa ggatgcagta
    48961 gaactagacg gagtcgatct attaggctat acgacttggg gatgtattga tttagtatca
    49021 gctggtaccg gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg tgataaccaa
    49081 ggaaatggca cattagcccg ttcaaagaaa aaatcattca actggtataa aaaagtaatt
    49141 gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag tctattgttt
    49201 ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga acaatagact
    49261 ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa tctacatatg
    49321 tatgatgtaa tcgttattgg cgcaggtcct gctgggatga ctgctgcttt atatgcttca
    49381 cgttcaaact tatccgtatt gatgatcgaa caaggtgccc ctggcggcca aatgaacaat
    49441 acggcagaaa tcgaaaatta tcctggattc gattcgatca tgggccccga tctagcttat
    49501 aagatgtatg aaagtgtctc aagatttggg acagaaaatg cttatggaat cgttcaagat
    49561 atcaaggatc acggcagcca taaagaagtc atctgtgaag ataaaagtta cgaaggaaaa
    49621 acagtcatta tcgcaacagg atgtatccac cgtaaattag gcgtacctgg cgaggaagaa
    49681 tttgctggac gaggcgtttc ttactgtgca gtctgtgatg gtgcgttttt ccgtaacaaa
    49741 cgtctagtag tcatcggggg tggggactca gcagtagaag aagccatcta tttgacacaa
    49801 tttgcttctg aagtagtgat cgtccaccgt agagatgaat tacgtgcaca aaagatcatc
    49861 caagaccgtg catttgcaaa tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa
    49921 atcgttggaa acgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa aaccggcaat
    49981 gaatttgatt tagcagcaga cggagtattc atctatgttg gactggatcc attaactgca
    50041 ccttttagag aagcaggttt gacgaacgaa gctggctgga ttccaacaga caacgaaatg
    50101 aaaacgaata tgccaggcgt ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa
    50161 atcactacag ctgttggtga cggcggtatt gctggacaac aagtgtataa atacttggag
    50221 tctttagaag aaacaacagc ataagtt
//
LOCUS       DACEOM010000011        49444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEOM010000011 DACEOM010000000
VERSION     DACEOM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.012"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..3655
                     /locus_tag="IW781_000625"
     CDS             128..3655
                     /locus_tag="IW781_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1181645.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3652..7374
                     /gene="addA"
                     /locus_tag="IW781_000626"
     CDS             3652..7374
                     /gene="addA"
                     /locus_tag="IW781_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1181646.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7443..7784
                     /locus_tag="IW781_000627"
     CDS             7443..7784
                     /locus_tag="IW781_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1181647.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8093..9136
                     /gene="pheS"
                     /locus_tag="IW781_000628"
     CDS             8093..9136
                     /gene="pheS"
                     /locus_tag="IW781_000628"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1181648.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9141..11561
                     /locus_tag="IW781_000629"
     CDS             9141..11561
                     /locus_tag="IW781_000629"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1181649.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11731..12741)
                     /gene="trpS"
                     /locus_tag="IW781_000630"
     CDS             complement(11731..12741)
                     /gene="trpS"
                     /locus_tag="IW781_000630"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1181650.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12995..13732
                     /locus_tag="IW781_000631"
     CDS             12995..13732
                     /locus_tag="IW781_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181651.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13746..14561
                     /locus_tag="IW781_000632"
     CDS             13746..14561
                     /locus_tag="IW781_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1181652.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14574..15221
                     /locus_tag="IW781_000633"
     CDS             14574..15221
                     /locus_tag="IW781_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1181653.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15237..15893
                     /locus_tag="IW781_000634"
     CDS             15237..15893
                     /locus_tag="IW781_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1181654.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16055..16876
                     /gene="racE"
                     /locus_tag="IW781_000635"
     CDS             16055..16876
                     /gene="racE"
                     /locus_tag="IW781_000635"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1181655.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16879..18234
                     /gene="rph"
                     /locus_tag="IW781_000636"
     CDS             16879..18234
                     /gene="rph"
                     /locus_tag="IW781_000636"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1181656.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18235..18753
                     /locus_tag="IW781_000637"
     CDS             18235..18753
                     /locus_tag="IW781_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1181657.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18860..19360
                     /locus_tag="IW781_000638"
     CDS             18860..19360
                     /locus_tag="IW781_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1181658.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19587..20651)
                     /locus_tag="IW781_000639"
     CDS             complement(19587..20651)
                     /locus_tag="IW781_000639"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1181659.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20648..21733)
                     /locus_tag="IW781_000640"
     CDS             complement(20648..21733)
                     /locus_tag="IW781_000640"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1181660.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21746..22723)
                     /gene="mvaD"
                     /locus_tag="IW781_000641"
     CDS             complement(21746..22723)
                     /gene="mvaD"
                     /locus_tag="IW781_000641"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1181661.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22716..23660)
                     /gene="mvk"
                     /locus_tag="IW781_000642"
     CDS             complement(22716..23660)
                     /gene="mvk"
                     /locus_tag="IW781_000642"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1181662.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23982..24635
                     /locus_tag="IW781_000643"
     CDS             23982..24635
                     /locus_tag="IW781_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1181663.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            24720..25625
                     /locus_tag="IW781_000644"
     CDS             24720..25625
                     /locus_tag="IW781_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1181664.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25627..26259)
                     /locus_tag="IW781_000645"
     CDS             complement(25627..26259)
                     /locus_tag="IW781_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1181665.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            26579..27103
                     /gene="infC"
                     /locus_tag="IW781_000646"
     CDS             26579..27103
                     /gene="infC"
                     /locus_tag="IW781_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1181666.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27175..27375
                     /gene="rpmI"
                     /locus_tag="IW781_000647"
     CDS             27175..27375
                     /gene="rpmI"
                     /locus_tag="IW781_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1181667.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27428..27787
                     /gene="rplT"
                     /locus_tag="IW781_000648"
     CDS             27428..27787
                     /gene="rplT"
                     /locus_tag="IW781_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1181668.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28039..29376
                     /locus_tag="IW781_000649"
     CDS             28039..29376
                     /locus_tag="IW781_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1181669.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29424..31553
                     /locus_tag="IW781_000650"
     CDS             29424..31553
                     /locus_tag="IW781_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1181670.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31528..32217
                     /locus_tag="IW781_000651"
     CDS             31528..32217
                     /locus_tag="IW781_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181671.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32625..33312)
                     /locus_tag="IW781_000652"
                     /pseudo
     CDS             complement(32625..33312)
                     /locus_tag="IW781_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(33448..33642)
                     /locus_tag="IW781_000653"
     CDS             complement(33448..33642)
                     /locus_tag="IW781_000653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181672.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33692..34132)
                     /locus_tag="IW781_000654"
     CDS             complement(33692..34132)
                     /locus_tag="IW781_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1181673.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34136..34981)
                     /locus_tag="IW781_000655"
     CDS             complement(34136..34981)
                     /locus_tag="IW781_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1181674.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35130..36548
                     /gene="pepV"
                     /locus_tag="IW781_000656"
     CDS             35130..36548
                     /gene="pepV"
                     /locus_tag="IW781_000656"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1181675.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36605..37552)
                     /locus_tag="IW781_000657"
     CDS             complement(36605..37552)
                     /locus_tag="IW781_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1181676.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37775..38125)
                     /locus_tag="IW781_000658"
     CDS             complement(37775..38125)
                     /locus_tag="IW781_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1181677.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38325..39404
                     /gene="pepA"
                     /locus_tag="IW781_000659"
     CDS             38325..39404
                     /gene="pepA"
                     /locus_tag="IW781_000659"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1181678.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39548..39868
                     /locus_tag="IW781_000660"
     CDS             39548..39868
                     /locus_tag="IW781_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1181679.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39890..40354
                     /locus_tag="IW781_000661"
     CDS             39890..40354
                     /locus_tag="IW781_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1181680.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40559..41164
                     /locus_tag="IW781_000662"
     CDS             40559..41164
                     /locus_tag="IW781_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1181681.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41203..42492)
                     /locus_tag="IW781_000663"
     CDS             complement(41203..42492)
                     /locus_tag="IW781_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1181682.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42920..43399
                     /gene="rlmH"
                     /locus_tag="IW781_000664"
     CDS             42920..43399
                     /gene="rlmH"
                     /locus_tag="IW781_000664"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1181683.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43484..43993
                     /locus_tag="IW781_000665"
     CDS             43484..43993
                     /locus_tag="IW781_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ1181684.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            44093..44743
                     /locus_tag="IW781_000666"
     CDS             44093..44743
                     /locus_tag="IW781_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181685.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            45194..46132
                     /locus_tag="IW781_000667"
     CDS             45194..46132
                     /locus_tag="IW781_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1181686.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            46145..47197
                     /locus_tag="IW781_000668"
     CDS             46145..47197
                     /locus_tag="IW781_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1181687.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            47463..48098
                     /locus_tag="IW781_000669"
     CDS             47463..48098
                     /locus_tag="IW781_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1181688.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            48289..49080
                     /gene="msrA"
                     /locus_tag="IW781_000670"
     CDS             48289..49080
                     /gene="msrA"
                     /locus_tag="IW781_000670"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1181689.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        15881 a         8781 c        10365 g        14417 t
ORIGIN      
        1 gcgtatactt aaaaaagtag aaatatcaga accttaaaag gatataattt gaagaatata
       61 gaacaaatgt tcagtatttt ttgatataat aaaggagcac cgtatcatgt gttgaaggag
      121 gaaaagcatg tctttacaat ttattcttgg aaacgggaaa aaagatcacc gtcaagtctt
      181 gatcgacgaa gcatccgctt ggctcgacca ggatgcaagt aaccaagtat ttttccttgt
      241 tcccaactac agcaaattcg aacaggaaca agaaatttta gcggagatgc gaagaagaag
      301 cggaaaaaaa actttcagca caacaaacgt tcaagtattt agtttttatc gtctggcttg
      361 gtatttgctg caacagacaa ctcttcttac aggaaatgaa ttatcagaat caggatctgc
      421 aatgatcttg cgacagattt tagaaaaaaa tgaggaagaa ttaacaatct ttcgcggaga
      481 aatcaataaa agagggttta ttcgtcaatt acaagaactc tacagtgaat tgcgtacagg
      541 aaatattcaa ccagaagacc tgatgctttt atttggtgat tcacctaatg cagaagacca
      601 gcaacttaaa atgaaagact tgaagctgat tttttcagct tatgacagcg aattgactca
      661 acgtgcacta acaaatgaag atgcactatc tatactagca aattatatgc agacacaaga
      721 tttcaacaac gtcaaattta ttgtcagtgg tttttctcga atcaatggtc aagagtatca
      781 attactgcag gcaatgatgg aaaaggggca attggttgtc gatttattgc tggatcgtcc
      841 ttatacagcg gatcttccag aagtacttac gctctttcat gagactggaa agctttattt
      901 ccgtcttttt caaagtgcaa gagaacggaa agtccctata ttagtggacc gatttgcacc
      961 agagcagact cgtccgacag agcttcaact gctgcaaaca atgtgggaag agacatctag
     1021 ccaaaagaaa gcggcagtac acaaaatagg gggacaagag catctccatt tgtgggaagc
     1081 tgaaaatcca actgaagaag tccgacaatt agctgttgaa atcagacgac tagtcagtga
     1141 agaaaactac cgttatcgtg atatccaagt gctgacaaaa aatatgtctt tatatggaaa
     1201 tatccttcgg ccgatctttc aagaaatggc aattcctttt tatatcgatg aagaacaaat
     1261 gatggaaaat catccgctga tcgaatggat caacagtctg ttcgctcttg accgctattc
     1321 ttatcggcta agtgatatta tgcgtttttt caaaactgaa ttatttctgt cgacggaagc
     1381 agaaaccgaa cttgatgaat ggaaacaaga gcgagatgaa ttcaggaaaa aactggacat
     1441 cactgaaaat gtagcacttg cttacaatta tcaaggaact tattggactc gtgaaaaaga
     1501 ctggcagttt gtggcgtatg attttgaagc agaacagatt gaagacgtac agtatttgga
     1561 agaacagtca aatacgatta gacgattatt tcagaaagct gtaccagtat ttttgaaaaa
     1621 aataaagaaa agcaaaacag gacaagaggc cgcaaatgtt ttttatcaat ttttgatcca
     1681 aagtggcgta cagcaacaat tattgttttg gcgtaatcag gaagtagaca gaggaaactt
     1741 agaagcggca agaaaccatg agcagacatg gggagcgctg attgatctgt tggatgaata
     1801 tgtagtaatt tatggtgaat cggcttttga ctggtcactg ttccaagaaa tcattacaag
     1861 tggtctagaa aatctaagtt atggtaaaat ccctaccgca atcgaccaag tgcgaatcaa
     1921 tcgtttggaa ttagttcgac caaatcaagc tgccgtcact tttgcattag ggttgaatga
     1981 tcaagtgttt ccagaccgaa aagagacaaa aggattgttg tccagtgaag aacgagaagt
     2041 attaaatcag gccttgccag atggacagtt tttattcgac ccatcaaagg aaagtatctc
     2101 ttttgagcct ttccaagcct atcttgtttt tctttccgga acagatcgat tatatcttag
     2161 ttatgcacaa agctatgaca cagatggttc gctgaagatg tctccttatt tgcgtagaat
     2221 cacagaatat ttatctgttc ccgtcgagag aaaagagcat ttgacgatcg aaagcgaccc
     2281 taattgttat gtagggactt acagaagcgg aatcaataca gtcaatagga tttatcgatt
     2341 agctttagat gaaaaaaaac ctgtaccaaa gcactggcaa aagctcaaag aattagtact
     2401 gaattcgtca tggcgaaatt ttgctttaca gatttttgaa agtcaggaac atttaaatgt
     2461 tcctgtttcg atgccagaaa aaatggcgga agagctttat ggaaaagaga tttatacttc
     2521 aatttcacgt atggagaatt tttataattg tgaatacaaa tacttcatgc aatttggttt
     2581 gaaattgaaa gaacgaactg tttatggtct aactccagca gcaactggtg atttttatca
     2641 tgaatcattg gatcgtttct tcaagctact gttttcaaac caattatcct tagtgaatat
     2701 gtccgatgcg cagcgtagag actttactga aaaagtgctg caggaagtat tcggagaact
     2761 tcggtttgag atattggata gttctgcaag aatgaactat atccgctacc agttaggaca
     2821 gacgattcaa aaagtgacat gggctttgca aaaacagagc aaaaatacag gcatgcatcc
     2881 tcttcagaca gagattctat ttggtcagat tgctggagca aaaggtatca caggtctgga
     2941 attaccgttg agtaatggtg gaaaactcca tgttcgaggg aaaatcgatc gaatcgatgt
     3001 agcttctgaa caagaagata cttggctaag cgtcgttgat tacaaatcta gcggacgttc
     3061 attcgatgtc acagaagctt attatggcat ggctatgcaa ttattgacgt atctggacgt
     3121 agcattgatg gatgcggtac gtttgactgg aaaggcggct gttaaaccag caggatcata
     3181 ttatttacat gttcataatc ctgtattgac ggagacagat gatgtagaga agaatacatt
     3241 gaaaaaattt agttatgatg gcgtttttgt cgatgatccc gctcttttag aagtactaga
     3301 tcattcactg gaagcaaaag aacaatcact gatctatcca gtgaaaaaaa atgcgaaaga
     3361 tgaataccaa aaaggtagtt tcagtaaaga caaatttttc acggaagaag aactgcaact
     3421 attcatggcg cataatcggg aaaatatgcg gttagctgga gatgcgatta cttccggaga
     3481 aatcaaacta aatccagctt ataaagataa acagcggatt gcctgcgaat tttgtccatt
     3541 cagaagcatc tgtacgtttg atgtgatgct gaaagaaaat aattaccacc gtctggagaa
     3601 aatagataag gaagaagcac tgcgtagaat attgaaaagg agtgaagaag agtgaatcaa
     3661 attcctttaa aaccgcaaaa tgaacgattc acagatgatc agtggcaagc gatttttgat
     3721 caaggagata atctattagt ctctgcgtct gccggatcag gtaaaactac agtgcttgtt
     3781 cgaagagtga ttgaaaaact aaaaatgggt tttgatatcg atgaattatt gattgtgaca
     3841 tttactgaag ctgccgcaag agaaatgaaa gaaagaattc aagaggcatt acaagaatcc
     3901 gtcaatagcg aaagtgaccc tgtacgtaga cagcatttta ctaagcagtt agttttattg
     3961 cccacagcga atatctcaac cttacatgct ttttgcctga cagttattcg acgttattat
     4021 tacttaattg atattgatcc agtctttcgc atgttaacag atgaaacaga aacgatcttg
     4081 atgaaggagg atgtgtggga cgagctgaga gaagcacttt atgcagaaaa tgatgaacga
     4141 tttttccaat taacaatgaa tttttctaat gatcgttcag atgacggctt gacgaatctg
     4201 gtgttttctc tttatgagtt tgcccgtgcc aaccctgacc cccaaaaatg gctagaacag
     4261 ttaagcgata attatcgttt gcctgagggg ctagcgaaat ctcgattata tcaaaaacaa
     4321 atccgtcctt tagttttggc agatatatac caatgtgtac agttatatga acagatgact
     4381 cagttagcac aaggtgaagg attagaaaaa atgcatgagc aagtagctgg agaacaacag
     4441 cagatcaaga atatctatga agcgttctcg caagatcggt tagaagaagc ttatgctggt
     4501 ttggaacaac taactttttc aacttttaag agcagtcgga aagcagaatt gaaagaaatt
     4561 tccaatgaag tcaagggcat gagggataaa gcaaaaaaac tgattcagca gataagtaaa
     4621 agttattttc ctgtttcccc aagccaaatg gaagaattaa cggacaaagc actaccgtta
     4681 gtcgaagaaa tgacaaaagt cacgcaaagc ttcatggacg gtttcagtat gcgtaaacgg
     4741 gaaaaaggtg tgctggattt caatgatctg gaacatttag cgttgcagat tctcacagaa
     4801 aaaacgaaag atgtatggct gccaagtgaa gcttcaaaac attatcgaaa aaaattcaaa
     4861 gaagtaatgg tagatgaata tcaagacgtg aaccaattac aagaagccat tttatactgg
     4921 ctaagagaac ccgatgatac aaaaggaaat atgttcatgg ttggggatgt caaacaatcc
     4981 atctattctt tccgtttagc agatcctagt ttatttatcg ggaaatacga gaatttctca
     5041 aagaaagaag gcggacgtcg aatcgttttg gctgagaact tccgctctag aaaagaagtc
     5101 ctttctttta cgaatcttat ttttgaacaa ctaatggatc cagcagtggg tcaaatcaat
     5161 tatgacgaag cagccaaact gatccaagga ttttcagact ttccagagaa tgaacagttt
     5221 gagccggaaa tcatgatcta tgaaaaagaa caggaagaat cagagattga gattccgacg
     5281 gatgatatac tggaagacaa aacagaaggc gaacttttta tgacaggact aaaaattcgt
     5341 caattaattg attcctcctt tatgatttat gataaaaaat caaaaaaaag tcgtccgata
     5401 gagtacaaag acattgttct cttgacacct acaaagaaga acaacttaac gattttagag
     5461 attttcaaaa cattggatat tccattagag atgaatgatg ctcaaaacta ttttcaagcg
     5521 acagaaattc gcacgatgat ctcgctgcta caattaatcg ataatcccta tcaagatatt
     5581 ccgcttgcgg ctgtattgcg ctcaccgatc gtgggtctga ttgaaccaga attagccagc
     5641 attcgattag ctaaccgtgc acatacttac tatgatgctg tgcttgctta tcaagcatca
     5701 aacgaagatg aattagcagc taagctggaa cattttggca agcaactaga gcattggcga
     5761 gaacttgctc gccgttcttc gatcactgac ttgttatggg atatctatta tgagacggga
     5821 tatctagagt atgtggtcgg tctgccagca ggggcacaga gacaagccaa tctttatgca
     5881 ttggttgatc gtgccaaagc ttacgagcaa agttctttca gaggattata ccagttcgtg
     5941 cgtttcattg aaaaaatgca agaaaaagat aaagacttag cagaaccagt catttcaata
     6001 gaagataatg ctgttcgcgt tatgacgatc catgcaagta aaggcttgga attcccagtt
     6061 gtatttttgt tagatatgac aaaggagttc aacttacaag acttgcgcaa tcggtatgct
     6121 tttgaagaaa aacttggagc aggaattcgc tatatggatc ctgaaacacg agtgttatat
     6181 gatacgttac cgtttcaagc catcaaattg gcaaagcaaa acaaattgtt gtcggaagaa
     6241 atgcgtaaat tgtatgtagg attgactcgg gcagaacaaa agcttttcat tgtcggttcc
     6301 tataaaaata aagagcaaat gatccaaacg tggtctgaag cagctgatca tgaggagtta
     6361 gtttttgatc ctgcgctacg tttgaagggc agaagcagct tgatgaattg gattggttac
     6421 ggattgatcc gtcatcctga aatgcagaaa tacttagagg aagaaatttc gacttctctt
     6481 cttcaacatt caaatgcaca gttttctatt tcatggatga atcagcaatc gatcattgaa
     6541 caaagacagt tgcttgctga aaaagaactg gttaacttag atcagcagat gaaagaagat
     6601 gaaactttat tagcagattc gttacaaaaa cgtctggcat atgaatatcc ttatcaagct
     6661 tcttcacaaa ccacaagtta tcaatctgta tctgaaatca aacgattatt cgaagatcca
     6721 gatgatacac aagaatcacg tttgactctg gaaagcagtc agaacaaagc agcatcccgt
     6781 caatttcgtt atacgcaaga acaactagca gaaccaaaat ttttgcaaaa agatcggcaa
     6841 gtgtcagcag ctgctgttgg aactgctacg catgctttgt tacaattact gccattagag
     6901 atgccaacta cagagagtat ccatcaaaaa ttacaggaat tggtaaggaa acggttagta
     6961 gacgaaaaag tcgcgaaaaa agtagatgtt tcttctatta tatggttctt tcaaacagaa
     7021 cttggtcaac agctgattgc caacaaagaa aacgtaaaac gagaacaacc tttttccatg
     7081 ctgcttcctg ctgacgaagt atttcaagac tatccaaatc aagaagatga actattaatc
     7141 cacggaatcg tcgatggata cctagaagaa aaagatcatc tcaatatcta tgattttaaa
     7201 acagatttta ttttacatcc ggatgatcca gcagaaatag atgcaatcgt ccaaaaatat
     7261 caaggacagc taagacttta ccaacaagca atgtcagaag ctttgaataa gccagtagaa
     7321 aatgttttcc taattttgct tcgagtgaaa caaattataa acattaacaa ataattataa
     7381 ttgagcaaac ttgacttatg ttttgtaagg attatgctat actctgggaa gaggtgagta
     7441 tgatggagct aacacagaca gaattcgttt tatgccctaa atttgaaaaa acattctcta
     7501 ttttaggaaa aaaatggaac ggattgatta tcgatgtact actcgaaaac ggtccgcaac
     7561 gctttgttga gcttgcggca aaaatcccga tggtcagtga tcgagtatta gtcgaacgct
     7621 tgaaggaatt agaaaaagaa ggaatagtca ctcgaaactt tgcctgtagc gaaaagagcc
     7681 gttcagaata tctcttgact gaaaaaggag cagacttgca aaaagcgatg cgtcaaatac
     7741 aagtttggag cgaaaaatgg atcacagagg aagaatgcgg ttgattttgt aaaaaaaatc
     7801 ttgtaaacta tagtccatac gaataagtaa agaacgatga cgaaaaagag tagtttggct
     7861 aaaccagttc agggagatct gtcgcagact gaaagcagat cctgtgtttg aaaaatgaag
     7921 ttcactttcc gagttcgtct gataaaatca gaccggcaat tgtcgttaac gaagtaagag
     7981 tgcggtgtaa gtcatttatc cgcaaattag gatggtaacg cgaagacctc gttccttaac
     8041 aggaatgagg tctttttgtt ttgtatttgg aatttaaagg aggaacaagc acatgtctct
     8101 acaagcacaa ttagagagct tgaaagaagc aacgctcgtc aaaatcacag cggcacaaga
     8161 tttaaatgaa ttaaatcaaa tacgtgttga aacgttagga aaaaaaggac cgatcacaga
     8221 agtattacgt gggatgaaag atctaagtcc agaagagcga ccaaaagtcg gaagtttcgc
     8281 aaatgagatc cgagatcttt tgacagaaga gatcgagcga aaaaaacaag tattagaaga
     8341 agaagcgtta aatgcagctt tggcaaaaga aacaatagac gtgacactac ctggtagaaa
     8401 agtaaaacat ggtacacgtc atatcgtgac acaaatcatg gaagaaattg aagatatttt
     8461 tgtgggtatg ggctatcaga tcgtagaagg atatgaagta gaatctgatc attacaattt
     8521 cgaacggatg aatctgccaa aagatcatcc agcaagggac atgcaagata ctttctatat
     8581 ttcagacgag atattgattc ggacacatac ttcaccagtc caagcacgga caatggaaaa
     8641 acatgatttc tctaagggtg ctctacgaat gatctcgcca gggaaagtct tccgcagaga
     8701 tacagatgat gcgacccaca gtcatcagtt ccatcaaatt gaagggctag ttgttgataa
     8761 aaacatcaca atgggcgacc ttaaaggaac attagaagtc gtaatgaaaa aaatgtttgg
     8821 ggaagatcgt gaaattcgtt tgcgtccaag ttatttccca tttacggaac catcagtaga
     8881 agtagatgtc agctgtttca aatgtggtgg tgccggttgt aacgtatgta aatacactgg
     8941 atggatcgaa attttaggag ctggcatggt acacccgaat gtgttgaaga tgtcaggaat
     9001 cgatccagaa gaatattcag gctttgcttt cggccttgga ccagaccgcg tagctatgtt
     9061 gcggtatgga gtgaatgata tccgtaattt ctatcaaaat gacttacgtt tcttaagtca
     9121 gttcaagggg gagtaagaaa atgttagttt catataaatg gctcaatcgt tatgtagatt
     9181 tatcgaatgt aacaccaaaa gaattagcgg ataagatgtc tgtgacagga atcgaagtag
     9241 agggagtaac ggttccagaa gaaggcttga aaaaaatcgt tgtcggtgaa gtcaaggaat
     9301 gcgtaccaca tccagattct gatcaccttt ctatttgtca agtcgatatt ggcgaagacg
     9361 aactgtctca aatcgtttgt ggagcgccta atgtgaaggt tggaatcaaa gtgattgttg
     9421 cacttcctaa ctcacgtatc gctggtaatg taaaaatcaa aaaaggaaaa atgcgtggcc
     9481 aagtatcaaa tggcatgatt tgctcattac aagaaattgg ctattcagat agtgttatac
     9541 cgaaagaata cgctgaaggt atctattata tgccacaaga agctgtaaat ggcgatccag
     9601 tttttcctta tttggatatg gatgattcga ttattgaatt atctatcaca ccgaatcgtg
     9661 cggatgcatt gtcaatgcgt ggcgtagctt atgaagttgg ggcaatttat cgtcagactc
     9721 ctacattcga tgacgaaaag ctggtagaat cctcacgtcc tgctagcgat aaaattcaag
     9781 tggaagttga agataaagaa gcagttcctg cttatcaaat cagaatcatt gaaggagtaa
     9841 gaatccagcc aagtcctcaa tggctgcaaa atcttttgat gaacgaaggc attcgtccaa
     9901 tcaacaatgt agtagatgtg acgaactata ttttgctttt attcggacaa ccactacatg
     9961 catttgacta cgacaaattg aaatcaaaag agatctttgt ccgccgtgca aaagaaaatg
    10021 aaacattagt aacactagat ggagaatctc gggaattgac aactgacaat ctcgtcatta
    10081 ctaacggcaa agaaccagtt gctttagctg gtgtcatggg agggttagac tcagagatca
    10141 cgaatgaaac aacaacagtt gcactagaat ctgctttatt tgatccaatt tcgattcgtc
    10201 gtacagcaaa acaatttaat ttgcgtagtg aatcctctgc acgttttgaa aaaggaatca
    10261 accatgcgac tgttggagaa gccagtgagg ttgctgcagc aatgatcgtt tctcttgcgg
    10321 gtggggaagt tctacaaggc gcggtcaaag gctcagagat caaagcagaa gacgtatcag
    10381 tgaagatttc acttgaacgc atcaatcaat atttaggaac atctcttgtc gttaaagaag
    10441 taaatgagat ttttgaagca cttggattta gctatgaaga aaaggagtat gtttattccg
    10501 tagtagtacc accaagacgc tgggatatcc aaatcgaagc agatattatc gaagaagttg
    10561 ctcgtattta tggatacgac cagttgccat ctacacttcc aagtggagaa acggttgctg
    10621 gaagcttgtc aaaagaacaa gaaactactc gtaaaattcg tacaatcttt gaaggaagcg
    10681 gattgagcga agcaatcagt tatgcattga caacagaaga aaaatcaaga cagtttgtta
    10741 taaatgaaag taatattaca cgtttggact ggccaatgag cgaagaacgt tctgtattac
    10801 gaatgaattt gatcagcgga ctattagatg atatcagtta taacactgcc cgaaaaaata
    10861 cagagattgc tttatatgaa attggtcggg tgttcttcca agaaaatgat ccacgtacac
    10921 atctgccgca agaagtaaaa catgcagcaa tcgctatttc tggtatttgg gaagaaaaag
    10981 attggcagac gaaagctcaa aatgtagact ttttcaccat caagggtctt ttagaaaact
    11041 tatttgaaca actaggcatc actgaagatg tccattatca aacagtgaca gaaatgaagg
    11101 aaatgcatcc tggaagaact gctgcgattt accttggcga aaaatttatt ggttttgtcg
    11161 gtcaagtgca tccaacgatt acaaaaagtt atgatattcc agaaacatat gttgctgagt
    11221 ttgatttatc tgctatagtg gaagctgctc aaaaagggat cgttttcgaa gcagtgacaa
    11281 aattcccagc agtcagccgg gacatcgctt tgcttgtaaa agaaactgtg acaaatcagg
    11341 aattaacaga agttatccgt tctgctgccg gaagattttt aacagatatc caattatttg
    11401 atgtctatca aggagaaaac attgaaaaag gacataaatc catggcgtat agcctgacat
    11461 tcgtcaaccc agaagcaaca ttgacagatg aagaaatcaa taaagcaatg gaaaaagtcg
    11521 aaaaagcgtt ggtagaaaac ctagaggcaa gtattcgcta agaaggaaaa tatagtaagt
    11581 aacgatcaag tagcttttga agaccataca tttgaaaaaa agatgtgaca caacttcgtc
    11641 acatctcttt ttttgatttt aaaaaaggaa tagcacagag acgagataaa aaaacaggcc
    11701 tttacagcct gcttttttag agtaaatgaa ttaatggaag taattgattc ccattgctga
    11761 ttttacttca gaaagtgttt gtgcagctac tttagcagct tcttcactac ctttacgtaa
    11821 gatttccata acagctgaag gatctttttc caattcttct cggcgcgcac ggatcggtgc
    11881 aaattcagct tccaatacat caatgaggta tcgtttgatc tttacatcac caagtccgcc
    11941 gcggcggtaa tgagctttca tttcttcaat tgcttcttta tctgttccga atacatccaa
    12001 gtaggtgaag accatattcc cttctacttg tccaggatct tgtacatgga tgtggtttgg
    12061 atcagtatac atactcatga cttttttctg taagacatcg gcaggatcgg agatataaat
    12121 accgtttcct aacgatttac tcattttgcc atttccatcg attccaggta atctacccat
    12181 tccttttggt gggaatacgc cttttggttc caccaagact tcaccatatg tatgattgaa
    12241 gctttgtacg atttcctgtg tctgctctag cattggtttt tgatcctcac caacaggtac
    12301 aagattcgct ttgaatgcag tgatatctgc tgcttgagaa acaggataga taaagaatcc
    12361 tgtaggcacg ctttcaccga attttttttg ttcgatttct gttttgacag tcggattgcg
    12421 ccgaacgcgt cctacgctga ctaagttcaa ataatacatg gttaattcag ctaattccgg
    12481 aatttgtgat tggataaata aagtagattt tgcaggatct aatcccacgg ctaaatagtc
    12541 taaagcaact tgcaagacat ttgatgaaac cttctcagga tttttagcat tatctgtcag
    12601 tgcttgcatg tcagcaatca tgacaaacag ttggttgtct tcatctgcct gcatagccac
    12661 tcgggttttt agagaaccga cgtaatgccc taaatgcagc ttcccagttg gtcgatctcc
    12721 cgttaaaata atatttttca aaaaagacac ctctttacga tttgtaagtc tgagcactta
    12781 atatcttacc gtttttcctg aaaagaaaca acagttccta aggaaaaaac aaggaaagag
    12841 gataaaataa ctaatagatt agaaatggaa gaaatattta tgggagaata aacgttctaa
    12901 gaaactttac aaattactaa actatcagat ataatgtatt ttattataca tctttaaaag
    12961 aaatcttact aaataataat gggagtgtaa ttctatggca atgattaaat ttgatcacgt
    13021 ggataaatat tatggcaagt tccacgcatt aaaaaatatc aatctcgaat tcgagaaagg
    13081 agaagttgtc gttgtcattg gtccttctgg ttcaggaaag agcacgatgc ttcgctgtat
    13141 caatggatta gagacgatct cttcaggtaa gctgctgatt aatgatactg acttgcatga
    13201 taaaaagaca aaactgacag aagtccgaaa aaacatcggc atggttttcc aacatttcaa
    13261 tttgtaccca aataaaactg ttctggaaaa catcaaatta gcaccaatca aagtgttgaa
    13321 gcaagaccag gcaactgcag tcaaaaacgc ggagaaattt ttgaaaactg tcaatatgtt
    13381 agataagaaa gattcttatc cttccatgtt atccggggga caacagcaac gggtggcaat
    13441 cgccagaggt ttagcgatga atcctgagat gctgctgttt gatgaaccta cttctgcttt
    13501 ggatccggaa atgattggtg atgtattaga cgttatgaaa aaattggctc atgacggtat
    13561 gtcaatgatt gtcgtgacac atgaaatggg ctttgccaaa gaagtggcgg atcgtgttat
    13621 cttcatggct gatggagaag tattggaaga cagcagaaat gtgagagact ttttcgaaaa
    13681 tccaaatgaa gtccgagcac agcaatttat cagtaaagtt atcaatcatt gataggagga
    13741 aaaagatgcg caggttaaaa aaaatacgcc tgattttcct tttaggcatt gcttttcttg
    13801 cactagccgg atgtaaagga aatgggttgg caaatgaaga tattttagag cgaagcaaat
    13861 ctaccaatga aatcatctgg ggtgtcaagt atgatactcg attatttgga atgatggata
    13921 ttgagagcag aactgtacaa ggatttgatg tagatattgc aaaagcaatc actaaaaaga
    13981 tccttggtga caatggaaaa acagaatttg ttgaagtcac ttctaaaact cggattccat
    14041 tattgaaaaa cggaaatatc gatgcaatta ttgcaacgat gacgatcaca gatgaacgaa
    14101 aaaaacaggt tgacttttca gatgtctatt tcgatgctgg ccaagctttg ttggtaaaga
    14161 aaggaagcca aatcaaaagt gtagatgatt tgaatgcttc tacaacggtt ctagcagtca
    14221 aaggttctac atcggctgcg aatatccgac agcatgcacc agatgcaaaa attttagaat
    14281 tagaaaacta tgcagaagca tttactgctt tacaatctgg tcagggagac gcgatgacta
    14341 cggataatgc gattttgtta gggattgcag atgaaaatcc agagtatgag ttagttggtg
    14401 gaacgtttac taatgagcct tacgggattg cgatcaacaa agggcaagaa aacttcttga
    14461 aagctgttaa ccaagcgcta gaggaaatgc atgcagatgg cacatacgat aaaatctatc
    14521 aaaaatggtt tccaaatgaa actgaaggaa aagtagagta agggggagag gcaatgagcg
    14581 aattgataca aacttatgga ccaatctttg tcgacgggtt caagatcaca ttgttttcga
    14641 gtgtcttagc acttgtgttt agtttgatta tcggaacgtt aatggcaatc ttccaattat
    14701 caaagaataa agtagtgagc gccttagcaa aggcatatgt agaatttttt agaaacattc
    14761 cattattaat catcgtgatg tttttttatg ttgtcgtacc gatttattgg tttaatctag
    14821 atggattcca agcaggtacg attggattaa cgatctatac atctgctttt attgctgaaa
    14881 cagtacgttc agggattcaa accgtaccaa aaggtcaaac agaagcagga ctatcttctg
    14941 gttttaccta ttcagaaatc atgcgttata ttgttttacc gcaagcattc aaaatcgtta
    15001 tcccgccatt aggcaaccaa tttatcaacc tcgtaaaaaa ctcatctatt ttagcaatcg
    15061 tagctggttt agatttgatg tatcaaggag acttgatcgc tagtgaaaca ttcaatacct
    15121 tcgatacgta tatcatcgtt gggttatttt acttagttat cacgctaccg ttatcttact
    15181 taatggttta tcttgagaaa aaatgggcgg ttcgttcata gaaggagggg aaatacatgg
    15241 attttagcgg cgctttttca tggatgaata tccgtttttt attggaagga ttaaaggtta
    15301 ctgttgaagt ttcattgttt tcgatcattt ttagtttctt gatcggtggt ctgacaggtg
    15361 tgattcgttt tgcgaatatc cctttctttt ccaaaatcct tggtgtagtg attgatatta
    15421 tacggaatct tccattatta ttgatcattt tcttcactta ctttgcctta cctcaaatcg
    15481 gcattcagat gaacatcttc tggtccgctg tcgcagcatt aacgattttt gagtcagcta
    15541 tgttgtcgga aattttccga gctggactca atgctgttcc aaaaggacag atggaagcag
    15601 gattatcaac gggactgact tatgtagaat cgatgcgaac gatcgtgtta ccgcaagcat
    15661 ttaaatcaat gatcccggca attgtgagcc aattgatttc actgatcaaa gatacttcat
    15721 tagcagtaat catttcttta cctgaacttt ctcatcaggc acgtattatt tatggacaga
    15781 acaccaatta tgtgcttccg atgttcatca tcatgacact tatgtacttc gtggtatgtt
    15841 acgcgctttc tctgctttcc ggttacttgg aaaagagacg gtacagttat taatagatga
    15901 ttcttttagc ggttgtggca aactaaagtc acaaccgctt ttcctctttc cattcgaaaa
    15961 aaagagattt cgattgtttt aagcaagtaa gagcacttga aaattcacgc taaaaagtac
    16021 acaataggga ggatagaatt tattgaaaaa aaggatgata cgattgacag ataatcgccc
    16081 tatcggattt attgattcag gtgtcggcgg cttgactgta gtaaaagaag ccctgaaaca
    16141 attaccgaat gaaaatattt tatttgtagg agacacagca cgctgcccat atggccctag
    16201 acccgcggaa caggtaatcc agtatacttg ggaaatgacg gattatctgg tggagcaagg
    16261 aatcaagatg ctggtgatcg cctgcaatac cgcaactgcg gtggctttag aagaaatcaa
    16321 agctgctctt tctattccag tcatcggtgt gatccttccc ggtactagag cggcagtaaa
    16381 aaaaacacaa aataaacaag ttggcattat cggtacgatt ggtacggtaa aaagtcaagc
    16441 ttatgaaaaa gcactgaaag agaaagtacc agaattgact gtgacaagtc ttgcttgtcc
    16501 caaatttgtt tcagttgtcg aaagtaatga ataccattca tcggtggcga aaaaaattgt
    16561 ggcagaaaca ttagctcctt taaccactaa aaaaatcgat acattgattt tgggatgcac
    16621 ccattatcca ttattacgcc ccatcattca aaatgtaatg ggagaaaatg ttcaactgat
    16681 cgattctgga gcagaaacag taggtgaagt atctatgctg ttagattatt tcaatttgag
    16741 caattcaccg caaaatggtc ggacattatg ccagttttat acaactggct ctgccaaact
    16801 tttcgaggaa atagctgaag actggcttgg aatcggacac ttaaatgtag aacatatcga
    16861 attgggagga aaataaggat gcgtcatgac gggagaaaag cccaagagct tcgcaaactg
    16921 gagcttcaaa caaatgtttt caaatatcca gaaggatcgg tagtgatccg ttttggtgat
    16981 acaacagtta tttgttctgc aacaatagaa aattctgttc cccctttttt gagagaaaca
    17041 ggaacaggat gggtgacagc agaatatagt atgctgcctc gagcaacgag tacgagaaat
    17101 cgtagagaaa gcagcaaagg gaaactttct ggtagaacaa tggaaatcca gcgtcttatt
    17161 ggtcgttcct tgcgtgcagt agtagacttg gaaaaattag gagaacgtag tatcatcgta
    17221 gactgcgatg tgatccaagc ggatggcggt actagaacag caagtatcac tggggcattt
    17281 gtggcattga aattagccat tgaaaaactt ttaagagaaa aagaattgtc agaagatcca
    17341 ataaaagaac atcttgctgc agttagcgta ggaatcttac cagatgggac ttgcgtcaca
    17401 gatttggatt accaagaaga ttccgcagca cttgtagaca tgaatcttgt aatgacagaa
    17461 tctggaaaat tcgtggagat tcaaggaact ggagaagaag caacgtttga tggtgaacag
    17521 ttgaatgaaa tgctcttttt tggaaaaaac gcgattgaag atttgatcaa agagcaaaaa
    17581 catgctttgc taacagagtt tgctcaaaat gatgagcgga tagaagaaac aaaaacaatc
    17641 atcattgcga caagaaatcc aggaaaagca gaagagtttc gaaatatgtt caaagaagcg
    17701 ggatatcatg taaaaacatt acttgactat cctgaattgc cagatgtgga agaaactggc
    17761 agcacatttg aagaaaatgc ccgattaaaa gcagaaacga ttgcacaatt gcttgatcag
    17821 ccagtcttag cagatgattc aggtttgaaa gtggatgctt taggcggtat gccaggaatc
    17881 tattctgcac gttttgcagg ggaacaaaaa agtgatgcgg ggaataacgc caaattatta
    17941 tatgaattga cagatgtgcc ggatgaaaaa agaacagcac aattccattg tacgttagta
    18001 ttcgcggcac ccaaaaaaga cagccttgta gtagaagcgg aatggcccgg tcgagtagcc
    18061 cgtataccat ctggcgaaaa tggctttggg tatgatcctt tatttatccc agaaggtaag
    18121 aaacaaacag cagcagaatt atcgagcgaa gaaaaaaaca agatcagtca tcgggctcaa
    18181 gcaatgaaaa aattgagtgc cgagtggaaa caatggctgg aaggagagag atagatgcgg
    18241 tatttagtag taagtgataa tcatggagac agagatatcc tcgttgactt agtagatcgt
    18301 tatcgttctg aagtggatat ctttttccat tgtggtgatt cggaattaga agcttctgac
    18361 ccattatggg atacattcca agtggttcaa ggtaattgtg attatggacc aggattcgaa
    18421 cagaaaaaag tgatccaaac tggtcaggat acagtattca tgactcacgg tcatttatcg
    18481 aatgtccgat ttggtttgac acagttagca atcgaagctc gagctgcaac tgccgatatg
    18541 gtgttttttg gtcataccca tcaaataggc tgtgagatgg atcagaaaat cctgtttttg
    18601 aatcctggta gcatttctca gcctagaggc ccgatccaaa tcccatcata tgctcttatc
    18661 gaaagtttag atgaccaaat ttctgttcaa tactataacc gtgcccatca ggcagttgaa
    18721 gagttggcct tttctttttc gagagagcgc tgaataaaaa aactctaatt tttaataaaa
    18781 ataagcttta ttttatgaat tttgcatccg gtttcggtaa aatagtagtg ttgaatgaaa
    18841 tagaaatgga ggccatcaga tgattggacc tatagtaaga gaaatgctgc tggaaaatca
    18901 ggaaacgttt ttgattcctg cagaaaatgt ggcaaatgtt atgtatcatc atccattgtc
    18961 ccatggttta cttgtgttat cgaaggtcgg ttattccaaa attcctgtat taggaaaaga
    19021 tgatcacttt gtaggactag taagtttatc agatgtggtc aataaaatga ttgacttgca
    19081 aacaatcagc atggacccgt tagaaggact aacggttgct gatgtgacag aaacagatgt
    19141 tccgacaatt gatgaaaatt gggaactaga agaagtactc catttgttag tcgattcgcc
    19201 ttttttaccg gttgtcacag aagatcggat tttcaaagga atcattacaa gaaaagagct
    19261 gcttaaagcc gtaaacttca tggtgcatga attagaacgt cgtaatatat tgtctccgaa
    19321 aatcgaagaa ttaaaagaac aaatcgatat cgtgagctaa agaacgagaa actaaaagat
    19381 aatcaaaaaa taagtagaaa aaacagcgta ccagttttga ctttatcttg aaaaagaggc
    19441 tgggacaaaa gcgtttaact ccaagaaata agaaggaatt cacgaaaatt gcttttcaaa
    19501 tttttgtgaa tttcagctta tttccgaagg agttgctttt acttccgccg tttgtacgtg
    19561 tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctcttttc tgtgcgtctt
    19621 ccatacttca ctaagtcgat ccctcttgct tcgcaccaat ccttcactgg tccggaaaag
    19681 atcagagatt gttggtgtag ggccgcggta ttcgttgcgc cgaccatagt ataaagcaga
    19741 cgaagctctt cttgccattg cttcatcagt ataatagttt cttctacgcc gtgattcatc
    19801 agatgcgtca gtacggttcc tgaagtaccc acggcatttg cgccgagaca aagggcttta
    19861 aatatgtcat aggcattgcg gatacctccg gaggctagga tttccataga agtgtctgct
    19921 tcatttgctt caagcaatga agagactgtc gattgccccc agtcagctaa ataactcagt
    19981 tcccgcttgg agcgacgggc gttttcgatc tgggtgaagc ttgttccgct tcgaccgctg
    20041 atatcaactg tatgaactcc taaagcagct agatcattga gcgtttctct tgtcatgcca
    20101 aaaccgactt cttttacgat gagggggacg tcaatggctg tttgagtttc ttgaataagt
    20161 actttccaat tggtaaaatc acggtctccc tcaggcataa ccaactcttg aggtgcattc
    20221 aaatgaatct gtaaggcatc tgcgtgaaac aatcggatag cttctttggc tctttcgaca
    20281 gaagtccctg caccgatgtt ggcaatgatt ttcccgtgag gatattcttg tcgcataatc
    20341 gtataagtat cggctaaaga agggactttc aaggcagcgc tgactgaacc agtggcaatc
    20401 atcaaatctg cttctcttgc gacgattgca agatcttggt tgattttttt tgttttttcg
    20461 cttccgccag tcatcgcatt gatataaaat gggctgctta gtgtaaaccc aactgcctgt
    20521 gtacttagat cgacatcaga aactgctatt tgcggcaaag ggttatggat gatccgtacg
    20581 aagtcaaatt cgtttttttg tttatcatga aaggctttag ctagtgaaac atgttcgtct
    20641 tttcgattca tcggttttcc tttctttgat cgctatagac atgtaacggc agtggagtga
    20701 tttctgcttt ttcccatgca ctcattaaag gaagaatgcc agatttctgg tcaacaatta
    20761 cgattccgca atcgccccca cctgcaccag aagattttgc ggctccttca tactgttcag
    20821 ctaaattaca caatttgttc aaagcaggcg tttcgatgac gaccccagtg attgcagata
    20881 aatcatgcag taattgtcgg ttttttcgaa tcatctgttg gatcaacgtt acattatttt
    20941 ctttaaaccc tttgatcatt tcgttgacac attctgtact attttttaag aaaagctgat
    21001 aagccaccat tttatcttct ctcgaacggt gaacttgatc gaccaaatca gaagtagagg
    21061 cagggctacc cgtccaacca atcaataaac gtaaatcttc aggagcaatc aatggctcaa
    21121 tggatagacc tggccaatcc agggcaagta actcactgat agaatgctga gtttcttgtt
    21181 cttggagcca aggatggtcg aaggttgaga aagcgatcca gccaccataa cagctagcag
    21241 cgatgtcgcc gcaggaacca ttatcttgaa ctgctaaatt ggctagtgct gcaatcttga
    21301 aaatctccaa ctgagataaa ttcaacgcgt aaaaaacatt caaggctttg accgttgcaa
    21361 cggtcacggc accgctagaa cccaatccat atttccggcc atttgaactg tctaactcac
    21421 ttgttacttt cagatcataa aaggataaaa gaatgttttt ttcttgtgca tacttttcag
    21481 tcaagcgaat agcagcaagg atataatgaa aaggattttc ccgaatatct aaaactaatt
    21541 ctccgttgcg tcttgtccaa cgtacaggca tcccactata ttgtgcagat tggatacttc
    21601 cgacttttcg tgcagattct acagtgactg tcacgaattg atcgactgca gcgataactg
    21661 ctggatggcc tgtttcaaca actgcatatt ctccggcaat atagagtttg cctggtgcag
    21721 atacttcaat cataaaaatc tccctttatt caataatcgc aattcctgga ccagcatgtg
    21781 cagtaattac ttgttctttg ctaaataatt tggtaaatgt ttcttgtacc tcagataaat
    21841 ggctgttttc cactaaaact ttgacattag ggccagcatc catcgtaaag tagcatggaa
    21901 tgccttgctt tctcaattgt cgcaccgcat ccatcgcttt taagctgtca ggagaccagt
    21961 aagtgaatgg aggctgggca gctaaagtcg ttccatgcat ttttagtccg tttctttcca
    22021 tcgtttctcc gagaagttgg aaatcttttg ttttgattgc ttgtttcaat tgatatagat
    22081 ccccttcaac ggaatctaac cagccttgat aaaaactgga tgtttcgact gtccgacgca
    22141 tcccatttct gctggacact tcttttttct ggtcgttgat caaaacgaaa accattgcta
    22201 aatcgtcttc gaaagaatcg gaagggactg gcttagcgta agaacttaag tcgtcatggc
    22261 ctttttccca ttcgacgaaa ccaccgaaaa tacttcggca agctgaacca gacccgcgtc
    22321 gagcaaatct tgaaagagag agatcatcta atccaagctt aagagcagta ttgcaagcgc
    22381 ctgctaaagc agctagcccg ctggcagagg aggctaatcc agctgcggta ggaacgaaat
    22441 tctgactgat tactgaagct tttagtgaga ggccggcttc tttacgaaaa aggtcaagaa
    22501 actgactgac tttttttgtt gctttttcgt cttgcaaggt gccatctaga taaaattcat
    22561 ctaccatata gctatcagaa aagatgactt ctgtctctgt ataaaatgca tccaatgtta
    22621 acgaaaggct gttgttcatt ggaaggatga gttcttcatt tttcttaccc caatatttga
    22681 ttagagcaat gttggtatat gcgcgtgctt tgcctttaaa cataggtatg tactcctaat
    22741 ccttgtatcc aagtttcagc agcacccgca tcttcaagtg cttgactgat ttcttgggct
    22801 tttgtttttg tttgtgctaa tgcaatcata cagccaccgc gtccaccgcc agttaatttg
    22861 gcacccaatg ccccggtatc ttttgctgtt tggatcaata gattaagaaa atcattgctg
    22921 attgtcagct tttccagaag tgattgtgat tcatccattg tctgtgccaa cgtttctggt
    22981 gaattttcaa tgattgcttg ttttgcttgc ttcgtcaagt atcctaattt ttggatcatt
    23041 tgtccagttt catgaggctg ggtttcaaag aggtgagcga catctttgac ggcttctctt
    23101 gtttgtcctt tgattcctgt atcagcgaca atcaaaaaag catcgatgtt caaagaaaag
    23161 tagtcgaaag gatgcccttt tgtaaaataa atcggctgca agctgctagt agcggctgca
    23221 tcgattccac taggattacc gtgggcgatt ttttccgaaa gctggacatt ttctaatagt
    23281 atttcacgag acaaaggaaa tgctaagtaa tcataaaaag cacgagtgac tgctgtggcg
    23341 actgcagcgc ttgatcccat tccacgttca gcaggaatcg tgctttcaat ggtcagttgc
    23401 aagttttttt ggatgtcatg ctgttttttt aagtgaatga ataatttttt gatatttttt
    23461 aatgcatgag gagcttcggc taaatttcct gaatagtagg aagaaaccag ttgatcgtcc
    23521 attgcatcca gctcttcaag gaatgcggtg acttttgttg catagaaagg aaaggcaatc
    23581 gctggttctc cataaacaac cgcgtgctcg cccatcaata tgatctttcc gcttgattct
    23641 ccttggccat agtttgccat aatctaactc cttctcacgt aaaagtgatt tttaagcaaa
    23701 aaataaagcg cccaaaacga gttgcgctgc ttcacgaatt tttttgtcca ctaagggtct
    23761 caaaaaacat tcctccttta ttttactaaa accatctcag aagtaaaact aagaatacgt
    23821 tttctaaaga aaaaacactt ttttcttaag aattttttga ctttttttct tctttgacta
    23881 gaaaaaaata caattgacac tactagatat tgggggtata tttaaaaagg ttggagttaa
    23941 acacaatatt tagtgatgaa gtgagctgga ggtaaatgag cgtgatcgtt ttagcaggta
    24001 caattggtgc cggaaaatca agtttaacag aaatgatggc tgaacacttt gatagtcagg
    24061 cattttatga atcgattgat gataatgaag tcttgccctt attttatgca aaccctgaac
    24121 agtatgcttt tttgctgcaa atttattttt tgaataaacg ttttgcaagc atcaaacaag
    24181 caatgaaaga tgacaataat gtattggacc gttcgattta tgaagattca ctgcttttcc
    24241 atctaaatgc agatctagga agagcaacag aaacagaagt tcgtgtgtat gatgaactgc
    24301 tggaaaacat gatggaagaa cttccctatg ctgcccataa aaaacatccg gatttattag
    24361 ttcatattcg tgtatctttt gatacgatgt tggagcgtat cgaaaaacgt ggtcgttcgt
    24421 atgaacaatt gtcctttgat ccaagtctat acgattatta caaagaattg aacagacgtt
    24481 acgatcaatg gtatgaagaa tacaaagaaa gtccaaaaat ccaaatcgat ggagaccgct
    24541 ataattttgt ggaagatcct caagcaaaag aagaagtctt gaaaatgatc gaagaaaaat
    24601 tagcagagat tcgccaaacc aaagtagcca tttgatttac agttgctcaa ataaagggta
    24661 aacctttatt tgagcgactt ttttctttat actggaaaca aatgtcagga ggatagagta
    24721 tgcgggaaaa acactatttt aatcaagacc gtaatttttg gatcaatgta cagcaaaacg
    24781 atgtgctagc tgttaaaaac ttaaaagaag aatatggcat tagtgacgag atgctgactt
    24841 attctctaga taaaaatgaa cgggcgcgcg tagagtacga ttcttttgat gaagcgttac
    24901 tgcttgtatc aaatgttccg caccagcaaa aagtcgataa tcattatgaa actagtccta
    24961 tcgcttttat tttaaaagaa gacggtttgt ttacatttac cacgccgaat accgagtacg
    25021 tgattcgact aattcgatcg cttttggaac gaatgccaga catgagtgta tacagtttat
    25081 tgtttcgtac attgtttttg atttctgatt ccttttttcc tttaatcgag gaagtgaata
    25141 gtgaacgtca gcggcttaat ctgaaattaa gagaaaaaac aacgaacaag aatctgctgc
    25201 aactttcaga tttagagatc ggtttggttt acttagttac aggaacaaaa caaaatgtag
    25261 tacttttaga acaaatcaag gcattggcta tttatcgcaa gttgtcagaa aaggaaaaag
    25321 aagagttgga cgatgcgctg atcgaagcac gacaagcagt tgaaatgacg aacttagctt
    25381 cgcagatttt agaccagctc tctggcactt ataataattt gctgaataac aacttgaatg
    25441 acacgatgaa atttttgacg gtctggtcgc tgatcttgac cgttccaact attgtgacag
    25501 gattcttcgg aatgaatctc cagttaccct ttacccattc cgttttcgga tggggaatcg
    25561 ccttgatcat cagtttggtc ttatctattt ggatgcttat tgcactgtgg cggcggattc
    25621 gttgatctag tctttgatct gcaaaccaag ggcttgggcg atcacaagtg cttgctcttg
    25681 gttgacgatt agtcccgcca aagaatcttg agataaagcg attgctgtga agaaattagt
    25741 cgtaaagtct aatcccttca gagaagttcg aaaccagttc gaaccggtca actgattgtc
    25801 agacaaaaaa aggtttttcc aagtgacctc ataaaattcg ctgtcttcta attgacaatg
    25861 atcgaaggag acagctttca agtttgtact actaaaggaa gccatcgaag caatacaatc
    25921 agtgaaagta cagtctcgaa gataactttc ggcaaaattc gtacctgtta atttacattg
    25981 atgaaacacg acttggtgga aactgccacc aatccactcc agattggaaa aatcacattt
    26041 ttcaaaaatg acattactgc attcaaaatg acgtaaaact gttttttgca tcgtaagttt
    26101 ttggatatga ctctctctta ataccatatt tttggcatcg agatagcttt ggtcaccccc
    26161 gttgatcaga agaccagaga agatctcctc atcttctaaa taaaactctc cctttgtaag
    26221 aagaggaaga gaaggtggaa taggttcgat ttttttcatg tgggttcacc tcgtttttct
    26281 ttagtatagc aaagaagcgg gaatcactgc tagaaagtct gctaatctaa tcattttcag
    26341 aaaaggaaga caaactgtat gaaaaacatt gacaaaaaga ttagtttttg ctatactatg
    26401 atggttgaga aataaaagca gaagcacccg cttctcgcct tagttgataa tgtcactggg
    26461 ctagatagtt ttcactggtt ctattaggtg aagtacgcgc gggttctgta agagaactcg
    26521 tttttttatt gccgtttatt cggtaagctt ttctttctct cataatattt ggaggtgaat
    26581 gaccatagca aaggatatga tggttaacga cggcattcgt gcacgagagt tacgattgat
    26641 cggttcagat ggtgagcaat taggagttaa gacaaaagca gaagcattga atattgcaga
    26701 acaagcaaac ttggatcttg tgttagttgc ccctggtgcg aaaccaccag ttgcgcgaat
    26761 catggactac ggtaaatacc gtttcgaaca acaaaagaaa gaccgcgaag cgcgtaaaaa
    26821 acaaaaagtg atcaatgtaa aagaagttcg cttaagtcct acgatcgact tgaatgactt
    26881 caatacaaaa cttcgtaatg cacgtaagtt cttggaaaag ggcgataaag tgaaagcttc
    26941 tatccgtttc aaaggccgtg ccattaccca taaagaaatt ggtcaaaaag tcttgaatcg
    27001 cttagctgaa gaaactgcgg atattgcaac agtggaacaa aaagcgaaaa tggacggacg
    27061 cagcatgttc ttgacgctgg caccgaaaaa cgacagtaag taatttgaat gactaactgg
    27121 ctgttggaca gccgatagtc ggataacgaa agattttgag gaggaaatat agtcatgcca
    27181 aaacaaaaaa cacaccgcgg atcagcaaaa cgtttcaaac gtactggtaa aggcggattg
    27241 aaacgcttcc gcgcgtttac aagtcaccgt ttccacggca aaacaaaaaa acaacgccgt
    27301 caattgcgta aagcaagcat ggtttctagt ggcgacttca aacgtattcg ccaacaacta
    27361 gcaaaaatga aataagaagt acaaataaca gaatctgaag atttattttt attaggagga
    27421 attatacatg gcacgtgtta aaggtggaac agtaactcgt aaacgtcgta aaagagtgct
    27481 aaaattagca aaaggttact acggttcaaa acacacttta tttaaaacag caaaagaaca
    27541 agtaatgaat tcatacaact acgcatatcg cgatcgtcgt caaaagaaac gcgacttccg
    27601 taaattgtgg attgctcgta tcaacgcagc agctcgtatg aatggcttga gctactcaaa
    27661 attaatgcac ggtttgaaag ttgctgaaat cgacatcaac cgtaaaatgt tagctgactt
    27721 ggctgttaac gatgcagcag catttactgc attggctgac caagcaaaag aagctttggc
    27781 taaataagct aaacaattat aaagaggaaa gaagataacg aaaagttatg ttctttcctc
    27841 tttttttatt tacaatcctc catcaaatga aacaggacaa taacgtataa gtctaaaata
    27901 gcataattaa ggttttttaa ttgaaaactg aacatttatg gtcacccttt aatgaaagcg
    27961 ctaactacat aagttagaat gagtttgact gatatattag tttgcttatc agaaaaacaa
    28021 aatggacgga ggaataacat gagtgatatt attattggta ttttgttagt tatctcattc
    28081 tttttcatgg tctggtactg tgtaaaaggc tacaacttga tggtcggctt tgccatcatg
    28141 gctactgtat ggatgggatt agcgctagtc ggaaacacgt tttcaccaaa cccggcgatg
    28201 gaagggcaag gagtaattga tgtgttgacg catatctata cgacggggcc agctgagtac
    28261 gccaaatcga tcttggtaaa tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat
    28321 agcggaatcg cagctaccct gattcgaaag gtagttgaac ttggagggga caagccaaga
    28381 atcacgatga gcttactatg tattgtaacc gctgttatat tcatgtcgat gacaggaatc
    28441 ggaccagtga tttctattgc agttattgtg ttgccaatct tgatgtcatt aggtatttca
    28501 gtaccggttg cactgttctc atttatgggc tcgattatgg ctggaatctt tgctaatatc
    28561 gtcaatttca aacaatatca aacgatttat gctggtttca atccagcggc tgaaagctat
    28621 acttataatg actatttcca aattggtatg attgggatga ttgtaagctt agttgtggta
    28681 ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct atgcaatggc agcaaacgta
    28741 ccagctgaag gtggcgacgc accaatgatc tcatggttag ccgtattatt accagtttta
    28801 ggggtcgttc ttttagatct tccaatcatc ttaggtttta ttctagcagg tatctgggca
    28861 ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa tctgtcgtca gtttgcgaaa
    28921 ttatttacag atggcgcagt agatgttgca ccaatggttg gtttcttgat gaccttagcg
    28981 atgttcaata attcagctgc gtatgcttct ccttattttt cagctatttt tggagattgg
    29041 ataccgcaat caccattagt cttggcaatc gtatttgcta tattgacacc actaggattt
    29101 ttccgcggtc caatgaatct agtcggttca ggctcagcta ttttagctgt cgtattagct
    29161 gttaatccaa cgatgtcgcc agcctttttg ttcccgttat ttgcaatcac aacgattgct
    29221 cctcagcatt tagatattac acaatcttgg gttgcttggg gattaggtta tacaaaagta
    29281 acttctcgag aatatatgaa gaaatccatc ccaactggtt ggatcatcgg agcgatctta
    29341 tgcctgatca cgttcttatt gtacgggaac gcctagtaaa caaaacgtcg ataaaaacga
    29401 ttagatgaat ggaggaaaag aaaatgaaac gttttgtgcg aatgggaatt gatgttggcg
    29461 gtacacatac gaaagcagtc gcaatcgata atgcgacgca cgaaatcatc ggaaaatctt
    29521 cagtgaaaac aacccacgat gatgtccgtg gagtagccgc aggtgtcgtt cagtcttttc
    29581 aaaattgttt gcgggaaaat aatatcagtc cagaagatgt tgtgtttgtt gcacattcta
    29641 ccacacaagc gactaatgca ctgattgaag gagacgtagc aaaagtcggc gtaatcggga
    29701 tggcaaaagg tggattagaa ggttttttag caaaacgaca aactagattg aacgatatcg
    29761 atttagggaa taagaaaaaa atagagattg tcaatgcatt cttacctgtc aaacacttaa
    29821 atgtagaccg tgtttcagaa acgatttctt ctttagaaag agaaagagcg gaggtcttgg
    29881 tttcttctat ggcatttgga gtagataatg gggaaccaga acgagttgtt tatgaagcag
    29941 catcggtaaa atcgatccct acaacgatgg cttctgacat cacaaaatta tatggattga
    30001 caagaagaac tcggacagca gcaatcaatg catctatttt accaaaaatg ctagatacgg
    30061 caacctctac agaagattcc gtacgagaag caggggtcaa tgtttctttg atgattatgc
    30121 gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa acgacctgtt ttaaccatgc
    30181 tttccggtcc agcagcatca gtcatgggct ctttgatgta cttgagagcg tctaatggtg
    30241 tttattttga agtaggcgga acaacaacca atatcggggt cattaaaaat ggacgtccag
    30301 caatcgatta ttcaattgta ggtggccacc caacatatat ctcttcatta gatgttcggg
    30361 ttctaggtgt ggcaggcggt tcgatggtac gggctaatca gtcaggaatc atcgatgtcg
    30421 gtccacgttc tgcacatatt gccggtctag attatgcagt atttacggag acagaaaaaa
    30481 tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg agaccctgct gattacgtca
    30541 aggtagtgat ggaagacgga gaagaagtga ctattacaaa tacatgtgca gccaatgtcc
    30601 ttggattagt gcaagaagaa catttttctt atggaaatgt cccatctgca cgaaaagcca
    30661 tccaagcatt agctgattat tgtcatacga cggttgaaga catcgcagaa cagatcatgg
    30721 aaaaatccta tgctaagatt gaaccggtga ttttagaatt ggcagacaag tatcacttgg
    30781 agaaagatca gatttctctt gtaggtgtag ggggcggcgc tgcctcattg attacttact
    30841 ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa cgccgaagtg atttcttcta
    30901 ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg tatcatccct tcgccaagta
    30961 aagaagatat acgatcgttg aaaaatgaag cgatgaataa agcgattgaa tcaggcgcta
    31021 caccagaatc gatcgaagtc cacgtagaaa tcgatccaca gacttcaaaa gtaacggcta
    31081 tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac caaggagatc acaacagaag
    31141 aagcactaga gcttgctgca gaggatatgc gattaaataa aaatgaagtt tgcttattgg
    31201 aaaatacacc tttcttttat gtatgcggcg aacagaatcg ttcaaaaaat gctggaagtc
    31261 ttcggatcat cgatcaaaaa ggatttatca aggtccaacg tggacatgct tcatgtatga
    31321 aaacaactgc agccaattat atgacagctg tcgaacaact ttgggaagat atggcagttt
    31381 atcaaactga attgattgcc cgaccagaat tttatctctg cttaggagca agagtctctg
    31441 attttacagc gacggatctg gaacaactac agcttttgat ggatctggaa gtatctacta
    31501 tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat caaacaaaca taagttagaa
    31561 gagttactaa acgaattaca acagatcccc gaagaaattt ggggattcta ccagtttcgg
    31621 cgagatttat tttggaaaaa gattcctttg tccaagcaga aaattcttat acaacaatca
    31681 atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa agtacccttt tgccgatgta
    31741 ggagaaattt gtgaacaaat ggctgttcca atcgtttcct gtgaatcaga gcaaataaac
    31801 gaacgaatca cttttgcaac ttatgcagaa gatgaaggga ttcgattgat gacggagccg
    31861 ttagaaaaat taaaatgttc aggactagct tcgatctcta aagaaacagc gcaagctttg
    31921 attattggac atgaactatt tcaccacatc gaagcttctg ttaagggaat ttatacgcaa
    31981 agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac atcaatcaaa cattcgtgca
    32041 ttgagcgaga tagcagcgat gagtttttca aaagagatga atcaaagcga tttttcgcct
    32101 tatgtattag aggcagtctt actttggcca tataatgaaa ttcgtagcca agggatctta
    32161 gaagaaataa aaaaaataga aaaaagatgt acggagtatg atttagatta caaataaaga
    32221 gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa taaacgatgt tctcctgtca
    32281 actcctcggt cacaagtcaa aatattcaac aaacatgaga agctgttttt tgaaattgtt
    32341 ccttgtgact cggagatgaa cggcaggctc acgacctctt ggaaaacggt gttcaaaagc
    32401 ttgcatctgc ggtaataagt ccagccaagt taaaaacata agaagctatt tttacttggc
    32461 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctcaataaac ggtgtgcaat
    32521 cagctgaccc tcggtattag ctcaaaaaaa acggcaaaat atgaggaact attttgccgt
    32581 ttctttctct aatactcagg tcaaaacgct gattccaaaa cctcttattt gatgggtctg
    32641 tcctgaatat cttcaaaata atcggggtaa ttatgaatca cgtattcttg ctcctcgata
    32701 atcatatgta aatgcatcgt gcaaagaaat ccgacttcat caaagttatg atcaatcatt
    32761 gattgaagca ttgtctgatg ttcatctaat actcgacccc agtctaattc tgaaatcgtc
    32821 aaacgtaacc atctgaaacg gttcaagtgc gtactataac tttctagcca atcccaaatt
    32881 tctcgttttc cagcaatttc ataacaagtt cggtgaaaat cagtatccaa atcaaaaaaa
    32941 agctttttta ttttgagcga ttactgcctg atcggtttgt tctagtattt tttctagttt
    33001 agcttgatct tgcttggtca ttttcgcact gcattcttgc aggatcggtt tttccaactt
    33061 ttcccgcgta taacgggcta atgaagcaga tcggatatcg atttttgaaa cataagtgcc
    33121 ggactgtgga atggttttta caagctcttg attgcgcagt tggatcagtg cttctcgaat
    33181 cggtgtacgc ccgatagcta gttgttcttc taacacttta tcggagattt tacttccagg
    33241 tgctaactcg gaataaatga tttgtttacg tatagattca taagcttgct gttgcaaatt
    33301 attgataacc atttaacctt cctttctatg cagatataat ctattataac ataaatcgct
    33361 tacaaatcgt gtgggcataa aaacacagtc tgcaaaaaaa gccctgatag gcatgcctat
    33421 tagagcctcg aaaagaaaaa tattgaatca atctggtcgt acctcttgta tcggtttgac
    33481 acgaccgccg attcttttag caaaagcttc ggattctttt ttgtctttga aaatgagcag
    33541 attgtctaag tttttttccc gagtgcgaca gccagtttta tcgatgtaac cgtgcattac
    33601 tttaacgaca tacatgggga tcacctgctt ttttcttttc attatacgaa ctactcgaca
    33661 caatacaaga aaaatgctta gacggtcttt attaagaatt tacatcatcc aaagaagcgt
    33721 acgcttggaa taactgatct aactccggat tgactttgct cagcttcact aatcgttgcg
    33781 aatcagaaag taaaaaacag tgtttactac tgccagtagt ttttaatttt ttgtcttcag
    33841 ttcccacaat ttcgtagcta aaatccaacc gtgtccccgt atacttttgg atagttggat
    33901 ggatgctcac tttttctccg taatggatca tttctttata gctgcaattt acttccagaa
    33961 cggggatgat gatcccagat tcttcgattt ttttataggg aaaccctaga tgtaccaata
    34021 aggctacccg agcctcttca aaccagcgga tataattgct gtgatgaata atccccattt
    34081 gatctgtttc ataataaaaa ggttctcgca cataacctgt aaatttctcc atgactcatg
    34141 cctcgttttc cattgatttt ttacaaaaat aattcatcca tttcggaagt gcttcgctga
    34201 tttgtgtcgg caaaacaata tattgttctt ttgccagctg gtctccaatg aaactatgaa
    34261 gataaatagc tgctgcaatc gtttggtcat tttgaggaaa ctgggctaaa aatcctgtga
    34321 tcatcccagc taaagtgtca cccatgccgc ccgttgccat agcagcagaa cctaaaggat
    34381 tggtgtagaa gtgctggtga ccataaattt ttgtctcatg acttttcaaa acgagcacag
    34441 caccgatttc ctgttgcttc tttttgtttt ctttatcccg ttgttcttgt atcggtagag
    34501 aactcaaccg ttgccactcc atttgatgag gtgtaagtac agtttgttcg ggatagggta
    34561 gcaaaagatg attagcagca agtaaactta aagcagagcc atcaatcacc agccattgtt
    34621 cttttgattg gttttctagt acttgtttga aacgttccaa actcttagaa gaagtaccca
    34681 atccgggacc aatcaagagt acatctgctt gctttattac ggaaagaaac tggtcagtat
    34741 ccgtccaaac tacaatcatt gcttctggca gatgactgtg aagagcactc cgattttttt
    34801 catcagttac tacagtcgtg agacccgctc cgctgtgtac ggcagctaaa gcactcataa
    34861 tgattgctcc gccatattgt tcattgccgc caatcaaaac gatacgtcca aatgttcctt
    34921 tatgtgaaat ttttggacga ggtgtgatga ctcttttcac tagttgttcg tttatcagca
    34981 tctaaaaacc tccttttact cattatagcg tatatttttt ttagagaaaa acagataaga
    35041 aaaggaattg tcagattttt tgaactattt ttttctgtaa tttgtggtat catgaacacg
    35101 aagacttaaa taagtggagg attaacttta tgacaattga ttggcaaaaa gaagtagaag
    35161 cacgtaaaga agacttgctt gaagacttga aaaatctttt acgtgtcaac agcgagcgcg
    35221 atgactcaaa agtaacacca gatgcaccat ttggacctgg acctcgcgac gcattgaaac
    35281 atatgttagc ttatggcgaa cgtgatggat ttaaagtaaa aaatgtcgat aactatgcag
    35341 ggcatattga tcttggcgaa ggagacgaaa cgctagggat atttggacac atggatgtcg
    35401 ttcctgcagg agatggctgg gacacagatc cttatgaacc agtaatcaag gatggaaaga
    35461 tctttgcacg cggatcaagc gatgacaaag gtccaagtat ggcagcttac tatgcgatga
    35521 agatcattaa agaacttgac ttgaaactat caaaaaaagt tcgcttcgta gtcggcagcg
    35581 atgaagaaag cggctggggc gatatggctt attatttcga acatgaagaa gaaccagatt
    35641 ttggtttctc accagatgct gaattcccaa tcatcaatgg tgaaaaagga aatgtttctt
    35701 tagctttacg cttcaaaggc gacaatgcag gagattatgt cttgaaatct tttgtttccg
    35761 gacttcgcga aaatatggtg ccaggaacag caactgcagc acttcaagta ccaagtgcag
    35821 atgcagctat cgcgatggaa gaagcatttt accaattcat cgaagcaaat ccagtaagcg
    35881 gaacgattga agcggacaat acgtatgtaa aaatcgaatt agttggtaaa ggcgcacatg
    35941 gtgcaagccc acaatctggt atcaatgcag gttctttctt ggcattgttc ttagataact
    36001 atgaattcct aggatctgct aaacaattta tccatgtagc agctgcttat gtccatgaag
    36061 atttttatgg agaaaaacta ggagtcgctt atgaagatga aaaaatgggc aaattgacaa
    36121 tgaatgctgg actatttgct tttgaagaaa atggtacaga ggaagcgaac ttcatcaata
    36181 tgaatttccg ttttccaaaa ggtgtaacag tagacggatt gcaaagtgac atcgaacaaa
    36241 cagtggggca agaaggcgca acagttactc gaggtgctcg cgtaatggaa ccacattacg
    36301 taccaatgga tgatccattg gtggctacat tattgcaagt ctatgaagac cacactggag
    36361 aaaaaggcta cgaacaaatc atcggtggcg gaacttacgg acgtttgttg aaacgtggcg
    36421 ttgcttatgg agcgatgttc ccaggatata ctgatacaat gcatcaagca aacgaattta
    36481 tgagtcttga tgacttgttc agagcaacag caatctatgc agatgccatc tatcgtttgg
    36541 cgaaataatt aattaaaaag aggatcaaga attgcaaatt atttttgcag tcttgatcct
    36601 ttttttactt tctactgtgg tattcatgat aaacatgata tagattcaat tcatattctt
    36661 tcttagcgtt cgtgacgact gtatccaaaa tcaaccctgt tactaatgaa agaagtgcta
    36721 aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag cttgtcagca
    36781 tatattcgcg aatcacagga atccccgtga ccaatccaaa taaaaagaag aagatggtcc
    36841 aaatcccaaa gaaaagcaaa ggcttataat ctttaaacat tttaataatc atcatgatca
    36901 ctttgaatcc gtcagagaat gtattcaatt ttgattcgct gccttcagga cgatcacgat
    36961 aatcgattgg gatttcaacg aacttgaatt ttttatctaa agcatggatc gtcaattccg
    37021 tttcgatttg aaaaccagaa ctcataatag gaaaactttt gacaaagata cgattgaatc
    37081 ctcgatagcc ggtcatgaca tcaagaattt ttgttttata cattctagta attgtatttc
    37141 taacaaggtt atttccaaaa tcgtggaaag gtctcttatt ctcatcaaag taggttccat
    37201 ttgataggcg atcaccaatg accatatctg cttcaccaga acgtaatttc tcgagcaaac
    37261 catgtacagc ttctgcagga taagtatcat cgccatctac cattaaataa tagtcggcat
    37321 cgatatcaaa gaacatttgg cggatcacat ttccttttcc ttgacgaggt tcttttttta
    37381 cgattgctcc accttgaacg gccaattcgt atgttcgatc ggtcgaatta ttatcataga
    37441 cataaatatc tgcttctggt aattctcgtt taaagtctgc aatgactgtt gaaattgttg
    37501 cttcctcatt gtaacaagga atcagaacag caattttttc ttgttgttcc atttttcact
    37561 ctccttaact atagaaaaca aaagctaaat taaaaatacc tcataatcaa aggaaatgct
    37621 agtttttccc gctaaatatc atttatttaa aaagaaaata ctggaatata tgtttggaaa
    37681 gctctatcta aaaaaccacc cactacaaaa gaaggacatg atacctctct tttgaatagt
    37741 gggtgcgtat tcgttctctc acttttttga cgtattaatc tgttgcacct ttttttcttg
    37801 aaatttctaa tacggttcct gtatgagcat ctgctaaaaa ttcataataa acagtttcgc
    37861 catcttctat tcgagaaaga cctccccgat aagcactcga ccgaacggcg aatttcctaa
    37921 taggttcctt ttcaaaagag atccaagaac cttcgattgc accttctttt agaaaagatg
    37981 acttgatatt ttcaagtact tggtctgctg acaatacttt tttgcgatga taccaaatcg
    38041 ttgaagctac cccgctcaat aatcctagac cgattccaac tgctaacccg cctttaaaat
    38101 agcttagttc gctttcttca tacatgtgga catacctcct tcttctcact tgttactcct
    38161 tgtgcttatt ttaccacact ctcatatttt ctttgaaata aataaccgct accagaaaaa
    38221 ataagctata ataaatacga tcggtctgca gaaaaaatca tattttatcc gcagatcaat
    38281 ataagtctga gaggagttta gtctgaaatt tagacgaata gcatatggaa gaaaaaacat
    38341 ttcaacgaat caaagagtta acagaattac aaggaacaag cggttttgaa caagatgttc
    38401 gcgcttatat ggaaagtcat atggaaccgc tggtagatga gttacagtta gatggtttgg
    38461 gcggcatttt tggtctgcgc catcatgaag aagcggatgc accccgtgta atggtcgcag
    38521 cacatatgga tgaagtcgga ttcatgctga cacaaatcca agaaaacggg ctttttaaag
    38581 tcgtacctct aggcggctgg aatccttacg ttgtttctgc tcagcgtttt acacttaaaa
    38641 cctctactgg taagaattat ccatgtattt cttcttccgt tcccccacat ttattgcgag
    38701 gaacaagcgg acaaaaatca gtagaagtaa ccgatatttt gttcgatgct gggtttgaat
    38761 caagagaaga ggcaatgagt tacggtgtcc ttccaggaga cacaatcgtt ccttacgctg
    38821 aaacgatcaa aacagccaac ggaaagaata ttatcagtaa atcatgggac aaccgctatg
    38881 gctgtacaat ggtattagaa gctttagaag cgttgcaaaa tgaaacatta ggtcatacat
    38941 tgattgcagg cgcgaatgtc caggaagaag taggcttgcg tggttctaaa gcttctgtca
    39001 ataaattcaa gccagatctg ttctttgcag tagactgctc tgcagcagat gatacagtga
    39061 cgaaaaatgg cacattcgga catttaggag aaggtacatt gatgcgtatc caagatccag
    39121 gtttgatcat gcttcctcgt ttgcgcgagt acttgctgga tatcgctgaa acaaacaata
    39181 tcccttatca atactttgtt tcaaaaggcg gtacagatgc aggtgctgct catacacaaa
    39241 acgaaggaat cccaagtaca gtgattggcg tagtgggacg ttatatccat acgcatcaaa
    39301 cgatgttcag tattcgcgat tttgaagctg cacgagaaat gttgattcaa acactgaaag
    39361 gtttggataa atcaacagtg aatacgatcg tatacggaaa ataatgcgat agtgaaacta
    39421 taaaatctgg caaaagattg ctttttgcca gatttttttt ataaaaagag gatcaatgtt
    39481 tgttagttcg aaagaaaggt gtataattta attgaaaaat acataaacag aaagtagagg
    39541 acaaagaatg attatcccaa atagtttaga agaattagca ggctacgtag aaactggaaa
    39601 gagtgtattt ttctttactg ccggctggtg cggagactgc cgttttatca aaccgcaaat
    39661 gcctgaaatt gaaaatgact tttctgattg gcaatttatt gaagtagacc gtgacaagta
    39721 catagatgta gccgctgaat ggaacatttt tggtatccca agttttgtcg tgatccaaga
    39781 cggaaaagaa ttaggtcgtc tagtgaataa agatcgtaaa acaaaacagg aaatcgaatc
    39841 gttcctagaa agtgtaacaa aaggctgaca tctgttagga ggggtcaaga tgaatcaaga
    39901 atataagaat attttagtag gtatcgacgg tagtgaacaa gcacttgctg ctttcaaaaa
    39961 agcggtagaa gtggctcgcc gtaataatgg gaaagtatat gtagcaaacg taatcgatca
    40021 gcaattttat aactttatgg gctatgcacc tattgatcaa aatctagtcg atcagcaaac
    40081 tgaaacagcc aaggaaatga tcgaagaatg taaagaatat gggaaatctg ttgattacac
    40141 aaatatagaa ggaattattg catatggttc tgttcgtgaa tccatggcag tcaaattgcc
    40201 aaaaaaatat gagatcgatc taatcatggt tgggcagtca ggtctaaatg cagtcgaacg
    40261 ctttatgaca ggaagtgtag ccagctacgt gatacgtcat tcaccttgtg atgtcttgat
    40321 cgtttctgac gaagaagaac agccaagtaa ttaagcaagt ggaacttgta cgaccttttt
    40381 aatcaattaa ctaaaacagt aaagggactg tgacaaaagt cttgtcacag tcccttgtta
    40441 cgaataaatg gtgttccaga agaggcttct tcggaaataa agcatgaaaa aaatttcatg
    40501 cagtgaatgc aattttttgt taagataaat ctcagcaaag gataatggag gaaagaacat
    40561 gatttttgct tataacaaag cacacgtcgg agacaccttg atggttatta tagccgacga
    40621 taaaggaaca gaaaacactg ttgaacgcaa atggaatgtt gctcggatca aagatgaaca
    40681 aggtcaagtt gttgcttgga actttttcca tatctccgat catctaacaa tcgaagggaa
    40741 cggccaagta agcttaacag atgaggaatt agcgaaattg aatcgattga tcaaagaagc
    40801 aggattcgaa gaacagcttg aagctgatca tgaaccgaag atcgttgtcg ggtacgtaaa
    40861 aacatgcgtc ccacatcctg attctgacca tttatcgatt actgagacag aagtagacaa
    40921 tggtacagta ttgcaaatcg tttgcggtgc acccaatatc gaagctggac aaaaagttgt
    40981 cgttgcaaaa ccaggagcta tgatgccaga tggaatgatg atctggccag gaactttgcg
    41041 aggggtagat agctatggaa tgatctgttc cgctcgtgaa ttgcatttgc caaatgctcc
    41101 agcaaaaaaa ggaatcttag tattgcctga ggatgccgta gtcggagaag catttccaaa
    41161 ataaagaggt tgtgacaagt ctttgtcaca acctcttttt tattaatttt cactattttg
    41221 attttgtttt aatgccgatt gatcgatcgt caattcaaca gtagttgttt tttctttcga
    41281 gccttcatag taagtgattt tgacagaatc tcccactttt ttcttataca aagcagattg
    41341 aagttctaca ccagaagaaa cttctttgtc atcgatctta gtaatgacgt cgtattgttt
    41401 caatccagct ttttcagccg gtgtagctgt ttggacattt gtcacgatga caccgttagt
    41461 tacagatgaa ggaatcttca agatttgttc ttgttgctga gtagaaacgg cagataagtc
    41521 gaccattgtg atacctagag ctggacgtgt cacttttccg tctttttcca gttgattgat
    41581 gatattcact acatcgttac ttggaatagc aaatcccatt ccttctacgc ttacattaga
    41641 agtagattca gaagtacttg cgatcttact tgagttgata ccgataactt gtccttcgat
    41701 attaactaaa ggaccaccag aatttccagg gttgattgct gcatctgttt ggatcgcatt
    41761 gatattgact gtttcgttcg attcattcgt acttgtcact tgtcggttca aggaagaaat
    41821 gattcctgaa gtgacagaat ttgcatattc agatcctagt ggggaaccaa tcgcaatcgc
    41881 aggctcacca accttcagag cacttgagtc accaaaagag gcaactgttt cgactttgtc
    41941 tgaattgatc ttcaacaccg ctaaatctga ataagcatct gttccaacta attcagcttt
    42001 taccttcgta ccgtctttca tcaagacttc aagaccttgc tggccatcta ctacgtgatt
    42061 attcgtgact acataagcag tattcccgtc ttttttgtag atcacgccgc ttccttcact
    42121 ataagcttcc aaattactat catctgagct gctactttct tcttgctgcc cgaatagtcc
    42181 gccaaaaccg cttgattggt tttgactttg taagttgata acagaaacga cagcgtcttg
    42241 aactttatct accgctttgg tgatatcaga atctacattg actttgacat tttccacaac
    42301 tgtttcaccg gcagaattct gattgccgct tgtagcagta gaactattgt tgccagtgcc
    42361 catcgctgca taaaaaatgc cagctgtcag tagtcctcca acgatccctc caacaagccc
    42421 cagacctaac ttacgccaga ggctattatt tttacttttt ttcatctttg gtgtcacatt
    42481 ttttctatcc atgtgttcct cctccaatta cattcattta tctactgtta gtatagtctt
    42541 atttgttgac tttagtctac tttgagactg tgaacttttt atgaaaaact ttaggaaaaa
    42601 tcattgtttt cagatgaaaa tatttgattt tctttttcca cagatactgt gtaaaactac
    42661 gcggatagga ttggaaaaat aagggataaa acatatccac agcctgtgta taaagtggat
    42721 aaattcttat aaaacgtatg tttgcctaat gaatgtgatg aaataaggta aaataacact
    42781 gttttctgtg gataaacctg tggatgttgt gcataactgt tggggaaatc tttttaacaa
    42841 aaatagccaa aataaacata tagacgaaaa aattgattat aataaacaaa gtgagcatga
    42901 agaccaaagg agagaaaaca tgaatatcaa aattatctca gtaggaaaat taaaagaaaa
    42961 atacctgatc caaggtatca atgagtatgt caaacggctg aatgcctatg caaaaatcga
    43021 attgatcgaa gttcctgacg aaaaagcccc ggagaattta agcgaagcgc aaatgagaca
    43081 agtaaaagaa aaagaagggg aacggatctt agcgaaaatc aaagaacaag aatacgtcta
    43141 tgccttagca attgaaggga aaaatcctac aagtgaatct tttgctaaac aaatcgacca
    43201 attaggtatc caaggaaaaa gccaccttgt ttttgttatc ggcggctctt taggactcag
    43261 cgaagccgtc atgaaaagaa gcaatgctca gatctctttt ggaaaaatga cgtatccaca
    43321 tcaattgatg cgtctgatcc ttgtcgagca aatctaccgt gccttccgga tcaatgcggg
    43381 ggcgccttat cataagtgat gcggttttta tcattgatcc taaaaatctt agcaagacaa
    43441 aaagtacgta agaaagtaaa gactaaatac ttcggaggaa caaatgaata acaatgatag
    43501 gttattgcga ttgcgttatg cgattgattt gaaagatacg gatttgatta aagcttttga
    43561 gctaggcggt gtaccactga ctaaagaaga agctcaggcg gtactgacca aagtacagga
    43621 taagaacaaa gataatgcag aaaataatgt gtatgaaaaa accatcaaca accaagtttt
    43681 tgacgcattc ctaaatggcc tgattctctt ggcgcgtggt ccgcaaaaag ataagccgat
    43741 agtagacacg ccgcaaaaaa gcaaggaaat caaatatatc aacaatgtgt tgctaaaaaa
    43801 attaaaaatc gccttgtcta tgaccactga tgatatttta gacgtttttg ctgaggcaga
    43861 aatctatcca tcaaaaggcg aaatcggggc atttttgaga aaagaaggcc aacgaaattt
    43921 taagccttgc ggagataaat atatgcgtaa ttttctcaag gggttaggaa tttataatcg
    43981 gagaaaagta taaagaaatt tcatgagaaa tcaaaacaat ggttgttttg atgagagcta
    44041 gtttttgtat gagattaaaa tattatctta atcgaggagc tggattggtt ttatgaatga
    44101 tgaatataag aatgatgaag ataagatgct ttttgaagag atagagaatc gatgtaggct
    44161 caattttgag cttcggggaa agatgtcttt aatacaacaa aaaaagtatt tagcgaataa
    44221 atcagagttt actctgggac atgttgaaaa attaatctcg gattggatta gtagtagaag
    44281 cgaatttaca aaaataaaac agccgattaa atttgacatg aaaaaacttt tgttaaataa
    44341 gagtgagata ggtaatagag atcaatatat tagagctaaa gggcaagaaa taattgattc
    44401 attaggtgaa atgaggtcat acaattattt gtatgtaaca catagagctg atggaatggt
    44461 cattactgtg gggaaatctt cttctaatga tattttttta gatggagatc ttttttatca
    44521 actcaataca aatcacttgt ctggcactga aaatataatt ttaaggacgg aatatggcaa
    44581 tgaaatattt gcaaaatatg atgagatttt aaaaaattat ttggactggg cttggataat
    44641 tccggttgag tcaggagatg ctaagaaact ggagagatta ttgggcgatg aactaattaa
    44701 taaaaaagta ccaattttaa attattattc ccatagacaa taaaaggcta tttttcatga
    44761 taatactagt tataactgta aaaatggaag aaaatctggg aaagaagttt ttaaataagt
    44821 gtattgttcg attttaataa ataataagca ataccatccg tctgtgttgc gcaacatcgt
    44881 actacaatga atattttttc acaaaaagaa aatcatccat cgaatgaatg gaaggtttta
    44941 gtatacacga ggttaatgcc ccctctttta taatcagagg agtgaggttt tttctatgaa
    45001 tgtttctatt ttcttattgc tagtctagtt tctcttacga tgaaattggg attctattcc
    45061 agtttttatt gatttgctga taaaataact tgaatggtgg atataaacat gataaataag
    45121 agaagaataa caaaacaagg ttgtgaaatg attctcaaat gaatataata gaaagaaaaa
    45181 ggaggaagaa tcaatgaaaa tagcaattgc aggcgctggc gcaatgggaa gtcgctttgg
    45241 gttaatgctt caccaaagtg ggaacgaagt attattaatc gatggctggg cagaacatgt
    45301 ccaacaaatc aaagagcatg gattacaagc aaattttaat ggtaaagagg tagaagcaaa
    45361 actaccaatc gttcttcaat ccgaagtaga aaaagaagat caagttgatc tgattattct
    45421 atttaccaaa gcgatgcagc tggaaaaaat gctgcaggat atccaatcat taatcaaaaa
    45481 agatacagag gtcttatgtc tattaaatgg tatcgggcat gaagatatta ttgagaaatt
    45541 tgtaccaatg gaaaatatct atattggaaa taccatgtgg acggcaggtc ttgaaggtcc
    45601 tggtcaggtc aaattatttg gaagtggttc agtagaatta caaaatttag gtgatggaaa
    45661 agaagcagct gcaaaaaaat tagcagacaa attgtctgaa tcaggattaa atgctcattt
    45721 ttctgacaat attcactatt ctatttatcg caaagcatgt gtgaacggaa caatgaacgg
    45781 actatgcaca atcttagacg ttaatatggc tgagctagga aaaacatcga ctgctcataa
    45841 aatggtggcg acgattgtca atgagtttgc caaagtagca gcagtagaga agattgaact
    45901 agatgtccca gaagtcattg cacattgtga atcttgtttt gacccggaaa caattgggtt
    45961 acattatcct tcaatgtatc aagacttgat taagaaccat cgattaacag aaatcgatta
    46021 tatcaatggc gcaatttcta gaaaagggaa gaaatatggt gttgcgacgc cttattgtga
    46081 tttcttgact gagcttgtcc atgcaaaaga agatagtttg aacgtaaaat aaaggagaga
    46141 ttgaatggaa acttcacaaa aaatatcacc gaaaattttt ctgaataaag tcctcgctgg
    46201 tacagcaaca ggaatcatta ttggattgat tcctaatgca gtattaggtg ccattttaaa
    46261 atattttagt gcgtatccct ttgcggtaac gatcgcacag ttagcagtta tttttcaact
    46321 agcaacacct ttgatcattg gtggtttgat tgccttacaa tttggatttg atccgatgag
    46381 aatgatggtt gtagccggag cttcttttgt tggttctggt gtagtcaaat tcacacctga
    46441 tttgggtgaa acgggtgttt acgttggtgc tggtacaggc gatattatta atacgatgct
    46501 gacagcatcg attgctgtcg ggctgatttt actgattgga aatagttttg ggtcagtgtc
    46561 gattgtgtta acaccgattg ttgtaggtgt aggtgcaggt ttacttggtt tttatttatt
    46621 cccttttgta acagcaatta cttctgcaat tggatcattg attaataact tcactacctt
    46681 acaaccgctg ttaatgtcta tcttgattgg ttgttccttt gcctttttga ttatctctcc
    46741 tatttcaaca gtagcaatcg gaatggcaat ccaactagat ggtgtttctg ctggtgcggc
    46801 tgcgatgggc gtagctgcaa ccacttttgt tttagtagtg aactcttgga aggttaataa
    46861 gtcaggtgtg acaattgcga ttgctttagg cgctatgaag atgatgatgc ctaatctatt
    46921 tagaaaacct gtgattctta ttccttgttt agttagtgca gtgattacag ctattcctgt
    46981 cgctctattt tctatttcag gtacgcctgc ttctgctggt ttcggcgtgg tcggacttgt
    47041 tggtccgttg gcttctttag atgcagggct aaatggcttt cttgttgttc tttgttggtt
    47101 agtcatccct gtttttgcgg cactaattac tcaattttta tgcgagaaag tatttaaact
    47161 gtatgatcga gaagaagtct tcaaattttt aggctagtag attttactaa gaaattttca
    47221 atcaatggat gcgtaagtga gatgtcaaca acatctgact tacgtttttt tgcctttttg
    47281 agtaggagat tgatgctgac aactgaactc ttcagtgcca ttcgattttt taaaatgtag
    47341 aagaaactca cctttatgtt attgattgtt caaaaggcaa ctagaataaa aatatgtgtt
    47401 aaaatgagaa aaaatttgat tgttggatgt atcgaataat cactaaaaag taggagaaga
    47461 aaatgatttt tttgcaaaaa tatggttttt attttttatt attaggtgtg attagtgatt
    47521 tatcgacacc gtatatcctt ggcctttttt atcccaaact gaatcaaatg actaccgtaa
    47581 ttagtgtgtt tggtgacgta gatagtccag tcagacgtgc tttcttggtc tggtcagttg
    47641 tatctggact tttttttgtt ctttcgttgc ctgcgttgta tcacctattt attggaacat
    47701 caaaaacact tgcaatactt gctgttgcaa cagttggttt atatgggata ggtgattgta
    47761 tttttactgg gttattcagt atcaatacga atgaaagctc ttggaatcta tcgacttgga
    47821 tccataatat aggttctggg ctaggctatg caggctttct acttttccca ttgttacttg
    47881 ttctgcttta tcgtcaaagt ggtcaaggta cgcttagcca tttttatctt gttttaacag
    47941 ttattagttt actaattgca ggtctttacg gattagcacg gattccgtcg attagtcagt
    48001 ttgccttctt taagcaattg ggcttctttc aacgcttaag ttttttcttt aactatttgc
    48061 cgatgatttg tttaagtttt tttcaactac gaaagtagaa aaagcagtgt gaagctgatt
    48121 aagtaaaaga atttctctat gggtaaccat tcgtgtggat gtgttcgact aagaatagag
    48181 atgaggagag cgaaaagcaa gttagtctta acttgttatg cgttgtgcta aatttcaggt
    48241 agtcattttt tgaatgaata agaagaaaac ttgaaagagg gtgacttcat gttagatgtg
    48301 gagcagatgg aaatagacct ttataatatt attttaaatc gaaatacaag agaatgggaa
    48361 agaacacaat ttgaagaggc aaaagaagac ctagcaaaaa tgtcgataaa gcaggtgata
    48421 gaaaaactag aggtcaactt gcgtccgcta gcactacgag ataatttgac accagatgcg
    48481 atggattttt atttaagaat cacaggggag tcagcgagtg caaaggctta tgattatgcc
    48541 aaacataaga taacttcacc cttataccaa gaacgggcga tttttgccgg tggctgtttc
    48601 tggtgtatgg ttgagccttt tgaaacaaaa ccaggtgtaa tctcagtgtt atctggttat
    48661 acaggtggag agaccaaaaa accaaattac gatcaagtca gtggtggata tactgggcat
    48721 gttgaagctg ttgagatcat atttgataca cgtcaaataa cctatgaaga gctgttggag
    48781 atttattggc aaatttcaga ccctacagat gcctttggtc aatttcaaga tcgggggaaa
    48841 caatatcgtt caattctatt tgttgttaat gaaaagcaaa ggcaattggc tgaaaaatca
    48901 agagaacaac tgattcaatc taaccgattt agccgtccta tcgttacaga gattcgccaa
    48961 gcaaagactt tttggccagc tgaaaatcat catcaacaat tttatcagaa gcaacctaaa
    49021 agatataaaa agatcaagca ttcgcatcag cttcatttat tttttcagcg acatagatag
    49081 aggaaggttg tggcgcaaag cgttcagctc cgacaaaata atgagcaact gacaaaaata
    49141 gcttttcata tttttggaca gttatgaatt atttcgagga gttgctttga gaacaccgag
    49201 gaaaaggttg tggcgcaaag cattcagctt caagtcgcaa ggaaacagga ggtatgacaa
    49261 gtcttttgtc acaactcctg tttttttact agcctttatt tccatgtgaa agagaattcg
    49321 aattgtagaa tcttgatttt tctatggaat tttgtagaga atcatcttga taattaatag
    49381 acacctaatc gaattccaca ttaatgatga actaaattcc gtataaaagc gcaaacaata
    49441 acta
//
LOCUS       DACEOM010000012        47930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEOM010000012 DACEOM010000000
VERSION     DACEOM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.013"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            388..576
                     /locus_tag="IW781_000671"
     CDS             388..576
                     /locus_tag="IW781_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1181690.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            654..1667
                     /locus_tag="IW781_000672"
     CDS             654..1667
                     /locus_tag="IW781_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1181691.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1734..2768)
                     /locus_tag="IW781_000673"
     CDS             complement(1734..2768)
                     /locus_tag="IW781_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1181692.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2883..5108
                     /locus_tag="IW781_000674"
     CDS             2883..5108
                     /locus_tag="IW781_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1181693.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRLLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5126..7009
                     /locus_tag="IW781_000675"
     CDS             5126..7009
                     /locus_tag="IW781_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1181694.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6990..7964
                     /locus_tag="IW781_000676"
     CDS             6990..7964
                     /locus_tag="IW781_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1181695.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            8623..9645
                     /gene="aroF"
                     /locus_tag="IW781_000677"
     CDS             8623..9645
                     /gene="aroF"
                     /locus_tag="IW781_000677"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1181696.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            9678..10742
                     /gene="aroB"
                     /locus_tag="IW781_000678"
     CDS             9678..10742
                     /gene="aroB"
                     /locus_tag="IW781_000678"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1181697.1"
                     /translation="MFTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            10744..11910
                     /gene="aroC"
                     /locus_tag="IW781_000679"
     CDS             10744..11910
                     /gene="aroC"
                     /locus_tag="IW781_000679"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1181698.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            11927..13018
                     /locus_tag="IW781_000680"
     CDS             11927..13018
                     /locus_tag="IW781_000680"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1181699.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            13031..14326
                     /gene="aroA"
                     /locus_tag="IW781_000681"
     CDS             13031..14326
                     /gene="aroA"
                     /locus_tag="IW781_000681"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1181700.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            14319..14828
                     /locus_tag="IW781_000682"
     CDS             14319..14828
                     /locus_tag="IW781_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1181701.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            14825..15661
                     /gene="pheA"
                     /locus_tag="IW781_000683"
     CDS             14825..15661
                     /gene="pheA"
                     /locus_tag="IW781_000683"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1181702.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(15724..17460)
                     /locus_tag="IW781_000684"
     CDS             complement(15724..17460)
                     /locus_tag="IW781_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1181703.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(17457..18386)
                     /locus_tag="IW781_000685"
     CDS             complement(17457..18386)
                     /locus_tag="IW781_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1181704.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            complement(18386..18964)
                     /locus_tag="IW781_000686"
     CDS             complement(18386..18964)
                     /locus_tag="IW781_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1181705.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            19237..20523
                     /locus_tag="IW781_000687"
     CDS             19237..20523
                     /locus_tag="IW781_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1181706.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            20660..21529
                     /locus_tag="IW781_000688"
     CDS             20660..21529
                     /locus_tag="IW781_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1181707.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            21526..22413
                     /locus_tag="IW781_000689"
     CDS             21526..22413
                     /locus_tag="IW781_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1181708.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            22580..23263
                     /locus_tag="IW781_000690"
     CDS             22580..23263
                     /locus_tag="IW781_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1181709.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            23260..24033
                     /locus_tag="IW781_000691"
     CDS             23260..24033
                     /locus_tag="IW781_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1181710.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            24030..24428
                     /locus_tag="IW781_000692"
     CDS             24030..24428
                     /locus_tag="IW781_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1181711.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            24595..25266
                     /locus_tag="IW781_000693"
     CDS             24595..25266
                     /locus_tag="IW781_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1181712.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            25325..25765
                     /locus_tag="IW781_000694"
     CDS             25325..25765
                     /locus_tag="IW781_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181713.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            25777..26445
                     /locus_tag="IW781_000695"
     CDS             25777..26445
                     /locus_tag="IW781_000695"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1181714.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            26509..27132
                     /locus_tag="IW781_000696"
     CDS             26509..27132
                     /locus_tag="IW781_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1181715.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            27188..27574
                     /locus_tag="IW781_000697"
     CDS             27188..27574
                     /locus_tag="IW781_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1181716.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            27787..29178
                     /locus_tag="IW781_000698"
     CDS             27787..29178
                     /locus_tag="IW781_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1181717.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            29525..30172
                     /locus_tag="IW781_000699"
     CDS             29525..30172
                     /locus_tag="IW781_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1181718.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            30565..33210
                     /locus_tag="IW781_000700"
     CDS             30565..33210
                     /locus_tag="IW781_000700"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1181719.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            33520..34833
                     /locus_tag="IW781_000701"
     CDS             33520..34833
                     /locus_tag="IW781_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1181720.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            34823..35476
                     /locus_tag="IW781_000702"
     CDS             34823..35476
                     /locus_tag="IW781_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1181721.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            35531..36223
                     /locus_tag="IW781_000703"
     CDS             35531..36223
                     /locus_tag="IW781_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ1181722.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            36373..36573
                     /locus_tag="IW781_000704"
     CDS             36373..36573
                     /locus_tag="IW781_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1181723.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(36827..38506)
                     /locus_tag="IW781_000705"
     CDS             complement(36827..38506)
                     /locus_tag="IW781_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1181724.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(38508..38720)
                     /locus_tag="IW781_000706"
     CDS             complement(38508..38720)
                     /locus_tag="IW781_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1181725.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            39113..40843
                     /gene="efrA"
                     /locus_tag="IW781_000707"
     CDS             39113..40843
                     /gene="efrA"
                     /locus_tag="IW781_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1181726.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            40840..42600
                     /gene="efrB"
                     /locus_tag="IW781_000708"
     CDS             40840..42600
                     /gene="efrB"
                     /locus_tag="IW781_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1181727.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            42695..42892
                     /locus_tag="IW781_000709"
     CDS             42695..42892
                     /locus_tag="IW781_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181728.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            43045..43530
                     /locus_tag="IW781_000710"
     CDS             43045..43530
                     /locus_tag="IW781_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1181729.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(43634..44227)
                     /gene="clpP"
                     /locus_tag="IW781_000711"
     CDS             complement(43634..44227)
                     /gene="clpP"
                     /locus_tag="IW781_000711"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1181730.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            44407..44934
                     /locus_tag="IW781_000712"
     CDS             44407..44934
                     /locus_tag="IW781_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1181731.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            45025..45762
                     /locus_tag="IW781_000713"
     CDS             45025..45762
                     /locus_tag="IW781_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1181732.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            45766..46683
                     /gene="miaA"
                     /locus_tag="IW781_000714"
     CDS             45766..46683
                     /gene="miaA"
                     /locus_tag="IW781_000714"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1181733.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            46685..47914
                     /gene="hflX"
                     /locus_tag="IW781_000715"
     CDS             46685..47914
                     /gene="hflX"
                     /locus_tag="IW781_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1181734.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        15373 a         8400 c        10391 g        13766 t
ORIGIN      
        1 tttttagtta gtctacatta ttttggcgat ttgccaataa ggactgttga gttgtgcaaa
       61 atcaaatctg ggtcagaatg aacgtcattc tgtctcagat ttttcgttat ttcaatttga
      121 ttaactgatt gatcaaaaag atttttattt tcatattctc aaatgattca acactaaata
      181 tacatgtttg acgcggaaat tattattcga ttttggcggc atcagggggg atgacgacgt
      241 agcagaccca ttattttact caagaaaatg tccgtgccta ttatttagaa tacgatagtg
      301 aacgtgctgg gagttttgtt cttttagaga aagtctctga agataaatta gttgtcttag
      361 attttgactg taacgaaaaa tggcgaaatg gaagagcacc agcaagtgat tgaccggatc
      421 tacgaagcga cacgatatct gcctttggat cgtttgtgct taagtcctca atgtggattt
      481 gcttcaacag aagaagaaaa tgttttgttt gaagaacaat ggaaaaaatt tcttttgtca
      541 aaagcattgc agaagaagtt tggggaaaat cattaaatca tctaggagag cttgatagga
      601 aataacacta tggtataatc agaatgctta atactaacta ctgagggagc cttatgaaaa
      661 agaaaattct taaatttatc ggatggttct tcggaatcat tcttgggatc gcgtttgcaa
      721 tctttcttgc cttccagctt tcccccagac ctggagcatt cttgatcaac catatgtttt
      781 caaatactgt tcaaatcact gataaaaaaa cgttcgatca agcagaaaaa aacgtggcga
      841 aaaaaacaga tttgacgtat gagtccaagt ataaagagaa tacttatgac atctactatc
      901 caaaaaacag caaggggcct gttcctgttc tattttgggt acatggtgga ggattcgtcg
      961 gcggagataa atcgggtgtc aaagaatttg cgacaaaatt agtttcagac gccaacatcg
     1021 cagtggttgc gatgaattat gaattagcgc ctgattcaga ataccctaat caagttcttc
     1081 aagtgaatga actgatcaaa gatttattag ctgaagaaaa agacgacaaa ttgctagaca
     1141 tggaacaatt attttttggt ggagatagtg caggttcaca aatcgcattg caatatgcag
     1201 ctatacaaac gaatgatgcg tatgctaaac aaatgaaaat caaacagcta cttccaaaag
     1261 atagtctgaa aggaaccatt tcttattgcg gacctgttga tttgaagcta acagcacagc
     1321 agcattcgga tgatcgattt atgaagtttt tcgtcaaaac cgttgcgtgg tcgttgattg
     1381 gaacaaaaga ttggaaaacg agtgagcaat tgaaagaagc tagtgtggct gaccatgttt
     1441 ctaaggaatt tccgccaacg tatataactg acggaaatgc atactcattc caggagcaag
     1501 gtcaagcatt agaaaatcgg ttgacagaat taaaggtacc cgtgcaatcc ttgttttata
     1561 cagatacaaa aaaagagatt acccatgaat accaatttga ttacacgcta aaagaatcgc
     1621 aaaattgtta ccagcagaca ttggattttg tgaataagta taagtaagag gttgtgaaaa
     1681 agccgtttcg ttttgagtat gaagaaacga agcggctttt cacaacctct ctttcaatac
     1741 ctttttgttc ttgggataaa actttcccta acaatcaatc gtgcaggata agtcaaaatg
     1801 attggttcgt cccgtatacc gtcaattcgc tccaatgcca ttcgaacagc tgcttgccca
     1861 attgatttcg cattgatttg aacactggtc aagccagggg agaggtttgc gattgtctcg
     1921 atattatcaa agcttgctac aacaatctct tctccaattt ggatattttt ttgtttcaat
     1981 gcccgataga caccaattgc cattggatcg ctcccgacaa gaatggcagt aggaagaggg
     2041 gattcatttt ctaatagatt agtcgcaagt acctcacctt gtttttctgt ccagccacct
     2101 agatgataag acatatattt ttctaagtga tgatcgatca taaaatgctg ataggcacgc
     2161 atcctctttt catttataga aatctgcttc ccaccgaatt catccatatc gactttatat
     2221 cccccgatat aggctattct tttatgacca ttttctaaga acaattccag tacggtttta
     2281 gtcatccgtt caagatctgc atacaccata tcgacctctt gctggatgtc agggttatcc
     2341 acgacgacta tttgcatgtt ttgttttaac aaggcggtaa tcgattcttt cgtaacagtc
     2401 ccaacaacga tcaccgcacc taactgatct agatcttccc attgtttagg attatcagat
     2461 agattataga ttcgattcat ccctaaatgc agacgtcgag cttcttcttc catccctact
     2521 cgcaaatgtt tgtaatagat atcctgttct tcgctcgctt tgccgatcgt agagatcaca
     2581 ccaatcgaag gcattctctt tttgatatat ttacgagtac tgatgtcata atccaacgca
     2641 gcagctgcct ctactatccg ttgccgagta gtctgagcca catggatcgt ctgatcgtta
     2701 ttcaacacac gggaaactgt agcaggagag acatttgcct ttcgtgcgac atctctaatt
     2761 ccaaccattt ttacacactc ctcaaaaaaa taaaaaattt ttattgacaa aattgtttag
     2821 taaacagtat acaggaatta taaaaaaaag taaacggttt ccgttttgtt gggagtgaaa
     2881 tgatggatat tacgcataga aaaacagata aaaatttcat cttatttgat gaaaagagcc
     2941 gagtcttcca tttacgaaat acatttcttt cttatctgat aaagatagaa gaaagcaatg
     3001 tattggcgca tatttatttt ggcaaaccag tcaagcagta caaagacaat aaaaattatc
     3061 cccgaagaga tcgtggattt tcaggaaatg ttcccttaaa tcctgaccgg tctctgtcga
     3121 aagatacact accgcaagaa tactcttctc atggcagcat ggactttcga acgcccgcat
     3181 cgatcattca gcgcaagaat ggatcagact tactggatct gagatatgac agccattata
     3241 ttacagatgg gaagccagag atcgaaggct tgcctcaaac ttatgtgatg gacaagtcag
     3301 aggcacaaac gttggtgatt tcacttaagg atagagaaac agcgatttat ttcgatttgt
     3361 tctataccat atttactgat agagcagtga tcacacgatc tgtaaagata agaaatgaaa
     3421 ccggagaaac aatcaaacta gaaaaagcgg ctagtttcca gctagacttt gcccatacta
     3481 ggcgatttga tgaagtcatt gctttgccag gagcacatgt caatgaaaga cagatttcac
     3541 ggcaatctgt tttatctgga acaaaagtat ttgaaagccg tcgaggaacg agcagtcacc
     3601 atatgaataa ctttattgct ttagttcatc atcatacgac agaaaatact ggagaagcga
     3661 tcggacttca atttgtttat tccggcaacc attctttcga acttgaaaaa gatcagatca
     3721 atcaattgag agtcgtcggt gggatcaaca gccatcgttt ttcgtgggaa ttgaatgcag
     3781 gccagtcttt tcaaacacct gaaatgatcc tttcttactc aagtcaggga ttgaacaaaa
     3841 tgagtcagat ccaccatgag ttgctacgtg aaagaatcgc aagaggtcgt catcaatttg
     3901 ctgaacggcc gattctggtc aataactggg aagccactta ctttgacttc aatagtgaaa
     3961 aaatcaaggc gatcattgat gaagcaaagg agctaggcat cgaaatgttc gtattagatg
     4021 atggctggtt tggaaaaaga gatgcggaca attcttcatt aggagactgg ttcgagtatg
     4081 aaggcaagtt gacaaatgga ttacgtgaaa tagcagatta tgctcatagc aaaggattga
     4141 aatttggttt atggtttgaa cctgaaatga tctcggttga ttctgaactg tatcgaacac
     4201 atcctgattt tcttatgcag gagccaggta gaatgccttc tgcttccaga tcacaacatg
     4261 tactggattt tacaaggtta gatgttcggc agacgattga gaaacaaatg agaaagattt
     4321 tagatacgat tcctcttgac tacatcaaat gggatatgaa ccgtttgctt tcagatgtct
     4381 attcgattac ccttgaccca cagagacaag gagaagtagc gcatcgttat atgctcgggc
     4441 tttatgaact acttgaacat ttgacaactg attatccgga aatcttatgg gaaggctgtt
     4501 ctggtggagg tggacggttt gatgccgggt ttatttatta tatgccgcag tcatggacaa
     4561 gtgataacac ggatgcagtg gagcgtatga aaatccaata tggaacaagc cttgcttatc
     4621 cgatttcgtc catcacagct catgtatcag ctgtaccaaa tcatcagacc gacagatcta
     4681 cttcgttgaa aacaagagga gaaacagcta tgagcgctgt ttttggttat gaactggatt
     4741 tgacaaagct gtctcctgaa gaaaaaaagc aagtaaaaga ccaaattata tcctatcaaa
     4801 cgattcgtcc ggtgatccag tatggccatt attatcgttt ggcgagtcct tttgaagaaa
     4861 atattgccgc atggatgttt gtgtctccta aacaagatga agctattgtg tttttaggga
     4921 ggatactagc ttcagctcaa ccagcgttcc atgaagtata tctgatgggg ttagatgatg
     4981 aggcacttta tcaggaacag acctcgaagc ggatattttc gggggccgaa ttgatgacag
     5041 ttggacttta cttccccgat tttcaaggtg atttccaaac agaactgctt catttcaaaa
     5101 agttatgaga gagaaggaaa aaagtatgaa agcaaatata aaatggttag atgatccaca
     5161 agtttttaga gtgaaccaat caaatgcaca tagcgatcat gtttgttatg caacaaggga
     5221 agaagtagaa accacatcct cccttaaaca gtctttaaat ggatcatggc gtttttgtta
     5281 ttcgccaaat ccttcggtac gaccaaaaac attttataaa aaagattttg atgcctgttc
     5341 ttttgatgaa atcactgttc ctagtcatat tgaattagca aattatgaca agatccatta
     5401 catcaatacg atgtatccgt gggagggaca tgtatttcgc cgtccccctt tcacgacaag
     5461 ccctgagaaa gaggcggaag ggatcttttc agctgctaaa gacaatccgg ttggttcgta
     5521 tttgacgact tttcgattag aagaaggtct aaagggaaaa caaatctcga ttcattttgc
     5581 tggtgtagaa caagcaatgt ttatttggtt gaatggtcat tttatcgggt atgccgaaga
     5641 ttcgttcact ccgtctgaat ttgatttaac accttatttg gaagaagaaa atattctagc
     5701 tgtggaggtt tacaaaagaa gtacagctgc attcttggaa gatcaggact ttttccgatt
     5761 ttttgggatc ttcagagatg tcttcttagt tgctcgagga caaaaccatt tagaagatct
     5821 ttggatcaaa cctcgcttag atgacaaatg tcaaaatggc aaacttgaag tagatttgtc
     5881 ttttgagcaa gtagccaaaa acagtcagct gaatgtagcg atactagatc ctgatgggaa
     5941 ggaaatctat caacagcaat atccgttaaa agagcaaatg acgattcgca tcgatggatt
     6001 tgaaaatgtg gcactgtggg ggcattttca gcccaattta tataagcttg aattggagac
     6061 gatagacgaa gaaaaaagag taacggagta tattgactat tccttcggtt ttcgtaaaat
     6121 cgaaatcaaa gataaaatca tctacttcaa tggaaaacgg atgattatca acggggtaaa
     6181 tcgacatgag tggcatccga aaaaaggacg cgccatcact atagaagata tggaaaaaga
     6241 tttggctatc ataaaaaatg caaagatcaa cggcgtacgg acatctcatt accccaacca
     6301 gatcacttgg tattatttat gcgaccaagc aggaatccat gtgatggcgg aaacgaacct
     6361 cgaatcccat ggctcatggc aaaaaatggg cgcatttgag ccttcttaca acgtaccagg
     6421 tgcagtacct cagtggcagg cggctgttct tgatcgagca aaaacgaatt tcgaaacatt
     6481 aaaaaaccat ccaagtattc ttttttggtc tcttggaaat gaatcttatg cggaagaaaa
     6541 cattcgacaa atgaacgatt acttcaaaaa agtagacccc gatcgacttg tccattatga
     6601 agggaatttt ccaaatccag cattcgaaga ttcgatttca gatgtaaaaa gctgcatgta
     6661 tgcgccacca gaagagatca ttgactacct tgaaaataaa gcggaaaaac cgttcatctt
     6721 atgtgaatat atgcatgaca tggggaattc gttaggtgga atggattctt acatgacgtt
     6781 gcttgatcgt tatgaaatgt accaaggagg ctatatatgg gattttaaag atcaggcgat
     6841 ccaagtgact gatccaatca caggcagaga agtgatgcgc tatggcggtg actttgacga
     6901 taaaccaagt gactatgaat tctcagggaa tgggatcgtt tttgcagatg gacaagaaaa
     6961 acccgccatg caggaggtaa gatattatta tgaaaaatac agtaaataaa agtcatatgg
     7021 atacggaaaa agttgcaatc gtcttcggcg actgtacatt aggtgtcaaa tcggggaata
     7081 cgcattatat tttttcttat acaagaggcg gactggaatc gctcaataaa aacggaaaag
     7141 aatggctata ccgggaaaca cttccagttt tttggcgagc aacgaccgat aatgacagag
     7201 gtagcggatt tggatttaaa tccggtatgt ggttaggagc cggattgtat ccaaaagtca
     7261 aagaaataga agtactggtg gacaataaaa agatcgattt gccgattgct ccagtaaata
     7321 atcaatattc aagtaatgaa tacgctgaaa cggttgaaat catttttaca ctggaaatca
     7381 acacagtacc aaaaacccaa gtgacaattt cctaccaaat cgctggagag ggtaaaatgc
     7441 ttgtcaatat gcattattat ggacaaaaag gattaccgga attacccgca ttgggtttaa
     7501 ggttcattat gccaacgaaa gcaaaatttt ttgagtatga aggcctatca ggggaaacat
     7561 atccggatcg gatggctggt ggtgtcgaag gtatttataa agtggcagga ctccccgttt
     7621 ccccatacct tgttccccaa gaatgcggca tgcatatgga aacgaaatgg gtagaactaa
     7681 caagaaatca aacgttggat aatacggata aagatcaaga agatttttcg ttgcgatttg
     7741 aacagcagga tacgccattt gctttttccg ctttaccatt caaagcagaa gaactggaac
     7801 aggcaacaca tatagaagaa ctaccgccag aaagacgaac ggttcttacg atttttgcaa
     7861 aagtaagagg agtcgggggt atcaatagct ggggagcgga tgttgagtca ccttaccgaa
     7921 tcagcagcga agaggaccat gaaatcggct ttattattca gtagcaatct ttagattatt
     7981 atttgaaaaa cggagtttac aagacaaagc tgctttattc tatatgtcat gaacaagaga
     8041 gtaaaaaagt tctcagatac ttttgttcat gactattttc ttcaataaaa ccaattgtta
     8101 tttttctgaa aaacaaagtt aactatcaga aaattaaaaa aagtttcata aaaatgattg
     8161 acagtcgatt tttttatgtc tatactaggt acaattctta catcagtttg gagcgatttt
     8221 ttatgaaatc tagaaaactt acaaaactga atacgtatta ctttagctat tttagatagg
     8281 tttgtattca tgacttcgtg ggtacaaaca aaccaggttt gtatctatga tggctaaaga
     8341 tttttaaaca cgacttacag ccacgtagag aaattcatct atagtggcta ttttatattt
     8401 ttgcagcgct ttttaaggct cgttgaagat gttcactgta gaggcaattt cctacaggga
     8461 catctttttt tgttgattat ctgggtcata agtaggaaag aaggagcgaa aatgagaaca
     8521 acaggacacg gtcatttagt aggaattaat gtaaaaccac cttgaaaaag tagagaaaat
     8581 cagccaagat agataagtaa acaaaagaaa gcgggaaaaa caatgatagt aattatgaaa
     8641 aaagaagcaa cggaagaaca aataaaaaaa gtcgtcgatc atatcgaaaa agaaggattc
     8701 aaagtctcaa tcgatcaagg aacagaacga gtagtgattg gactaaaagg tgatacgcga
     8761 agtttacaag aaggagcatt tacacgctat gaaggagtag aaaatgctgt gcgtatttta
     8821 aacacataca aattgacttc tcgagaattc catcctggca atacagtcgt ggatgtagac
     8881 ggagtaaaaa ttggtgacgg cagtttcgtg acgatggctg gtccttgttc tatcgaagga
     8941 ttagaccaaa tcagagaatg tgcgcgaatg gccaaagccg ggggcgcaaa aattctgcga
     9001 ggtggtgcct tcaaaccacg tacttctcca tatgctttcc aaggtttaga agaagaagga
     9061 ttgaaataca tccgtcaagc ggctgatgaa tttggaatga aagtgattac tgaagtgatg
     9121 gatgaagggc atatcgatat ggtggctgag tacagcgata tcctacagat tggcgcacgt
     9181 aatatgcaaa attttaaact gctatctgct gtcggtaaaa caggtaaacc tgttggattg
     9241 aaacgtggaa tttccggtac gatcaatgaa tggcttaatg ctgcagaata cattgcagtc
     9301 gaagacaaat ctcctgtcat ttttatcgaa cgtgggattc ggacttacga aacagcgaca
     9361 cgtaatacgt ttgatttaag tgctgtccca ttgatgaaaa aattaactca tttccctgtg
     9421 attgttgatc caagtcatgg tacaggaatt tgggaacttg taccaccaat ggctcgtgcg
     9481 ggtgtggcaa gcggagcaga tggcatgatc gtagagatcc atcctgatcc tgcaaatgct
     9541 tggtcagacg gtccccaatc gttgaatgaa aagacttact cacgcatgat gaaagaagtt
     9601 gcgatcatga aagaagcaat ggaaaagatt cagacactcg attaattcca gctttttaaa
     9661 agaaagaagt gaaacgaatg tttactgtac atgttgataa ccaaaaatac gatattcata
     9721 ttgaaaaagg cgcactagaa caggctggat catggcttca aacgatttgg agacctcaaa
     9781 aagtagctgt gatcactgat acaaacgttg ctccactata tgcagaaaaa atcatgttcc
     9841 aattgagaca agctaatttt gaaccagtcc tttcaatcgt accggcgggt gagaccagta
     9901 aatctttaga acaagcagct gagttatacg attttttagc agataatgat tttacccgca
     9961 gcgatgggat acttgcactt ggtggaggaa tggtgggtga tctagcagca ttcgtggcct
    10021 cgacgttcat gcgtgggatc catttcgttc aaattccgac tacgctactt gcacaagtag
    10081 attctagtat tggcgggaag acagcagtca atacaaacaa agcgaaaaac cttgtcggaa
    10141 catttagcca gccagctggt gtattgattg atcctgaggt tctaaaaact ttgccggtac
    10201 gtcgtttacg agaaggaatc gctgaaatca tcaaatcagc agctatagca gatgcaacgc
    10261 tttggaaaca gctgaacgag ctgaaagacg aaaaagatct tctggaacat gctgttccaa
    10321 tcattactgc tacgttggaa atcaaacgaa aagtagtgga agaagatgtc tttgaccaag
    10381 gaaatcgact tattttaaat tttggtcata caattggcca tgcaattgaa aatacagcag
    10441 gatatggggt agtcagtcat ggagaaggag tagctattgg aatggtagcc atcacttctc
    10501 atgcagaaaa aatcggcttg tctccaactg gatcgacagc tgctttagaa aggatacttc
    10561 aaaaattcca tctgccgatt acgtacgatc taagtcatcc agatgaatta ttccacgcga
    10621 taacccatga taagaaagca agaggaagcc aattgaatat catcttgcta gaaaagattg
    10681 ggcaagcaaa aatcgttcct gttcccacag agacattcaa agattactta ggaggactgt
    10741 aagatgcgtt atatgactgc gggagaatcc catggaccag aattaacagc cattattgaa
    10801 ggactaccag caggattacc tctgtcagaa gaagaaatca atcacgaact cgctcgcaga
    10861 caagctggtt acggcagagg aggaaggatg aagatcgaaa aagatcgtgt tcggatcacg
    10921 tctggcatcc gtcacggcaa aacaattggt tcacctgtca ctttagtggt agaaaataaa
    10981 gattggaaaa attggacaac tgtcatgtct gtagcagaag tgcctgaaaa agaccaatca
    11041 ctgcggcgag ttgcacgtcc gcgtccagga cacgcggatt tggtaggcgg tatgaaatac
    11101 catcatcgag atcttcggaa tgtgcttgaa cggtcctctg caagagagac gaccatgcgt
    11161 gtagcaatcg gcgcaattgc caagaaaatt ctggaagaac ttgacatcat cgtagcagga
    11221 cacgtgattt gtcttggtgg aatccaagca gaaatacctg gaaatatctc gattcaagaa
    11281 atcaaagaaa tttctgaagc gtcagaagta cgagttgttg atccagcagt tgagcaaaga
    11341 ataaaagatt tgatcgatca gacaaagaaa aatggagata cgatcggagg aattgtagaa
    11401 gtgctcgttg gaggcgttcc agctggatta ggaagctacg tacaatggga cacgaagcta
    11461 gatgccaaaa tcgcacaagc tgtggttagt atcaatgcct ttaaaggcgt agaatttggg
    11521 gtcggattca cttctggttc attaccaggc tcagaagtga tggatgagat cctatgggaa
    11581 gaagaaagag gctatttccg acgtaccaat aatctaggcg gcttcgaagg cggaatgacc
    11641 aatggcgaac cacttgtcat tcgcggggtc atgaaaccca ttcctacatt gtacaagcca
    11701 cttcaaagtg ttgatatcga tacaaaagaa ccttacaaag caagtgtaga acgttcggat
    11761 agtacagcag ttcctgcagc tagcgtcgtt gcggaaaatg ttgtagccac tgtggtagcg
    11821 acagagctat tgtcaaaatt cccaagtgat tcgcttgttg agttgaaagc tgcagtgaag
    11881 gagtaccgtg actacttgaa acattactaa aaagggggca aataagatgg cacaacatgt
    11941 ttttatcgtg gggcttggac tgattggtgc ttctatggca ctttgtattc gtcagaccaa
    12001 tccagaactg atagtagttg gttgggatag tcagtcaaaa acccgtgagc aagcagtaga
    12061 acagcagatc gttgattacg ttgcaagtga ttttgaaaca ggtgcagaac aagcagacgt
    12121 tatcttatta gccgttcccg tccgtacaac cttagcttat cttgatgtcc ttgaaagaat
    12181 caacttgtct gatcacgtca tcattacgga tgtcagcagt acgaagcagc aagtcgtgac
    12241 atccgctgag cagaaaaatc ttcggtttgt cggtggacat ccaatggctg gttctcataa
    12301 atcaggtata caggcagcag atgctaaact atttgaaaat gcctattaca tctttacacc
    12361 tttggaaaag acgaaaaaag atgttgaaaa actgcaagaa ctattttgcg gaaccaaagc
    12421 aaaatatgta actcttacag caagagaaca tgaccgtata acaggaatgc taagtcattt
    12481 tccacatatt ctggcagctg gcttagtcaa tcaggcagaa caattcaatc aagagtatcc
    12541 gcgagccaaa caactggcag caggaggctt tcgagatatc actaggatcg cttcctcaga
    12601 tcctgtcatg tggacagata ttttgttgag caataagcag atattattgg aacgattggc
    12661 agattggcaa caagaaatga cacaaatagc agaatggatc atgactgaga accaatcgga
    12721 aatcttttct tttttcaatc gggcaaaaga aagtcgagac cagttgcctg tccataagca
    12781 aggcgcgatt cctgcctttt ttgatttatt tatcgatgtg ccagatgaac ctggcgttat
    12841 tgcagaagta actggactga tcggaaaagc gggagtttct ttgattaatt tgaagatcca
    12901 agagaccagg gaagatattt taggaatact gcaaatcagt tttaaaaacc aacaggattt
    12961 gttacaagca aaaagaatca ttatgtctaa taccagttat cattgctgga tcaaatagga
    13021 ggctggataa atgcaattat tacaacaaat acatggatta agagggactg ttaggatacc
    13081 agcagataaa tcaatttccc atcgcagcat catgtttgga gcaattgctg aaggaacgac
    13141 gactatacaa aattttttgc gcgcagaaga ttgtctgagt actttacatg ccttccaaca
    13201 attaggtgtc gagatcgaag aagaggaaga ggtgatcaag attcatggtc gcggtagcca
    13261 ctcatttgtc caaccaaccg aatcaattga catgggaaac tccggtacga ccatacgttt
    13321 attgatgggt attttggctg gacagccttt tacgactact ctgttcgggg atgcttcgtt
    13381 gtctaaacgt ccaatggggc gagtgatgga acctttacgc gagatgggcg ctgacttgca
    13441 aggagaagaa aatgaccagt atctaccaat cactgtgaca ggaacccgct ctttatcacc
    13501 tatccgatac aatatgcctg tagccagtgc gcaagtcaaa tctgctttgc tgtttgcggc
    13561 attacaagca gaaggcacat ccgtaatcgt tgaaaaagaa cgttcccgta accatacgga
    13621 agaaatgatt cgtcaatttg gcggaaggat cacagtggaa gataaaacga tcatagtgac
    13681 cggaccgcaa aaattaactg gtcaacagat aactgttcca ggtgatattt catcagctgc
    13741 tttctttcta gcagcaggac ttcttgttcc ggaaagccaa ctgttgttaa aaaatgtcgg
    13801 ggtcaatcca acaagaactg gtatcttaga tgtgttggag gagatgggcg cagcgattac
    13861 ccagacgaat cacaatgaac agaaccaatc ggctgattta agtgtcaaga cgagtcattt
    13921 gaagaaagca acgattggtg gagaaatcat tccgcgtctg attgatgaat tgcccattct
    13981 agctttagtg gctactcaag cagaaggaat cacgatcatc aaagacgcag aagaattaaa
    14041 agtaaaagaa accaacagaa ttgatgcggt ggcagaagaa ttacagaaaa tgggagcaaa
    14101 gataaaagcg acagcagacg gtctgatcat ccatgggccg acacctctgc atggcgcaaa
    14161 agtgtccagc agaggcgatc atcgaatcgg tatgatgctg caagtagctg ctttgatagc
    14221 tgatggacct tgcgaactag aaggagctga agctgtttca atctcttatc cggcattttt
    14281 tgacgattta gcagaacttg tgtcaggagg agaagctcat ggctagcatt ttgctgattg
    14341 gtttcatggg cgctggaaaa acaacaatcg gtaaagggtt agctcaatgt ttacaaaaag
    14401 agtatgtaga tttagataca aagattgagg aacatatcca attgtcgatt gccgaatatt
    14461 ttcactatta cggtgaaaaa agctttcgaa aagtggagtc agatatttta agaaagctgt
    14521 caaatgaaga caaaattatc gcgactggtg gagggattgt ccaaagtgca gaaaatcggc
    14581 gttttttaaa aacacagccg attgttcttt acttagaggc agaagcggac tgtctagttg
    14641 atcgtatcca acaagatgaa acgtctattc gacctttagc tcttggaaaa acaagagaag
    14701 aaataaaagc attattggca caacgacttt cttggtatga agaaagtgcg acacatcgaa
    14761 tcaacacgac caatcaaaca ccagaaaaga tcatcgatac cattatagaa aggatcaaaa
    14821 gcatatgaac gtcagctatc tagggccaga aagttcattc acttatcagg cggcttgtca
    14881 gctatttcct acagaacaat tgactgcgta tgcttccatc cctgtttgtt tgcgcgcttt
    14941 gtttcgcaaa caagtcgatc tggctgtcgt tcctgtagag aattcattgg aaggagctgt
    15001 tcatcacaca atcgatcttc tttccaaaca tccagaagtc gaagttaaaa gtgaaatcgt
    15061 tcttcctatc aagcagcagc ttttaggcaa tccagcaaca aagattacaa aaatcctttc
    15121 acatccacaa gcactagcac aatcacaaca gtttttagaa acgcattatc ctaatgttcc
    15181 tttagtagca acagaatcaa caactgctgc agcaatgtat gtagcagaac atccgaaaga
    15241 agacgcagca gcgattgctt cattagaaac agcacagcat gttggattgg agatcttagc
    15301 tgagaatatc caagataatg agttgaacca gacacgtttt tggatcgtcg gtgaccgaaa
    15361 gatgaccagc cagcagtcag ctccagtaaa aatgagtgtc atcttgacac tacctgctaa
    15421 tcggccaggg atgcttcata aaatgctggc agcttttggc tggcgggaaa tcaacttaag
    15481 taagattgaa tcccgaccat tgaaaactag cttgggagaa tattttttcg tgatcgattt
    15541 gttgttggac cgtccaatga cattagtaga gaatgcgctc caagaaatca agatgctcgg
    15601 aggagaaagt caaatattgg gctgttatcc agtgctgaca gtagaagaga cgcgtagatg
    15661 aaagtcaaga atactgattg atttatttaa cttaaaataa aaaaatcagc gcttggctat
    15721 tctttatttt tgatattctg attgattttt aggaagaagc ggtagacaaa tcgttacagt
    15781 cgttcctgtc ccaatcactg attggatcgt taagccatat gcatcgccat acattaggcg
    15841 tatccgctca tctacatggc tgatgccgat tcctgaaaaa tgatcatttt tttgtttggt
    15901 attttcagta tcgaagccat cgccgttatc aatcacttcg aataaaagaa gctgattttt
    15961 tgttttgccg tacaccgtga tgaatccttg cgttttattg tgaaaagcat gaagataggc
    16021 gttttcgata attggttgaa ggatcatatt cggcatagat aatgactgca gttcatcagg
    16081 aatcattatt ttagccgaga ttctttcgcc atatcgcata ctcaaaagat cgatgtaact
    16141 ttgtacgctt ttgatttctt ccttgactgg aatcacttct tcttttttcc caatcgtact
    16201 tcttagtaaa gtaatgaaac tgtcgattcc ttgaactgct tcatctgttt ttttctgcca
    16261 aatcaaaaat ttcaagctag tcagcgtatt ataaataaaa tggggctgta tctgtgcttg
    16321 catagtttgg atttcaagcg aacgtttctt cttttcgtct tggatcgtct gttcataatg
    16381 atgctgagca ttacttagca attgattgat cgtattccct agcaattgga cttcatggct
    16441 tccttgtaca ggaacagtag cttttaagcc atcttcctct agccgtttta gcgaatcaat
    16501 caagcggtag atcgattttg tgtgtcgatc caaatacata aataccaatg agccagcaaa
    16561 agcaagtgtc acgatattat acaaaataat cggaaaaatc acgcccattt ggttagataa
    16621 agaatgaaga tcgacttgac tgagtaatgt acaatcctgg cggtacagag gaagacgggt
    16681 caactgctgt ccatttgaaa tcgttgtttc acctactgtt agttttttga aaatcattga
    16741 tcggctccca attgtatttt cttcatttga tgcgatgatc gtctgttctt cgttcatgaa
    16801 ggagatcttc gatatctcag gatttactac agattgaaaa agattcgcta gttgcttgga
    16861 tgagatcgat aaaacagcat atccgtaaac gtgattcaat gtatttttca gttttttgac
    16921 aaagaagacg ctgggtgctt tcttatcgcg aagagtcaat ccgtggtgtg caaaaagcat
    16981 ttgtgtatca gcagcacgtt cattcactct ctgagcaatc gcagattgaa aaaaagaatc
    17041 ggcagatacg gcagtgagca tgtcgtttcc gacaaaagtt cttccatttt ctccaaagat
    17101 caatacatcg taatcgatca ttggatggat ttttgaaaga gagtcattca ttttttggat
    17161 tgttcttagt tgctgaagct gttccatatg ttgggctgga tcattggaaa aatagctttt
    17221 gacagctgga ttagaatcaa aagcggctaa tgtattctcg attgtatcat tcaaactttc
    17281 aaattcagat tgaatctttt ttagtaccac atcattcgat tgattgaaca gctggatata
    17341 ggcatccctt gatcgatgat aaataaacaa agaaatgacg atggcttgca gaaaaagacc
    17401 gatgaacaaa atgaacagaa gtttggcaaa gagttcatgc tctttgattt tttttctcat
    17461 tttacaaaag ttcctttacg gtattgagaa ggtgtcattc ctgtctcttc tttaaaaact
    17521 ctggagaaat aacccagttc ggtatagccg actttctcac agatttccgt caaagtaagc
    17581 cgtgtttccg tcaaaaagag cttggcttta ctgatacgga ctttttttag atattttgaa
    17641 aagttgatac caaatttttc agtaaagatg gctgagagat agctatatga aaaatgatat
    17701 ttttcggata aaagttgtaa attgatttct ttctcataat tttcctggac gaactcgtag
    17761 atcaaagtca agatatcgaa attgtctttt tttgcatttg cagaaaagtg ggtgagtttc
    17821 tcaagtgttt ctttgacaag aatagaaaaa tcttcgaaat actcagcatc ggctaataaa
    17881 gaaggaaggg cgatcttgag caaagaaagt tcttcattcc caacatgctc gtcagccaaa
    17941 gcgttgagta atgcatagaa acgattgatt gcctcatgtt tcaaataagc tggatcaata
    18001 ggctgttcca atatcgtcgt gaagaaattc tcaagttctt tgacagctaa agcgtagtct
    18061 ttatgaacga tttttgttaa ataactactt gcttgctgct cgtggttggt taataacgga
    18121 agaagttcat gctgataagc aaggttcttt cccttatgat agaaacgttg tcctttggtg
    18181 caatttctga tctcttggaa tatttcattg atatcttgga ttgcaaaaga atcagaaaca
    18241 acaaaaaaat cacctggaaa aacttcttga cgaaactttt gaacaaactt cctaagtgta
    18301 tctttggaat cagtattgat tacatagccg gattcttccg aaaaggtaga aacaggataa
    18361 atagtaaacg aatcgtcgct atccatcaat gaatgggtga tcgactgttt ttgtttagag
    18421 ccttgttttg aactgaatgg ccgtttttcg gtatataaca gaaaaacaag gtgtgttcaa
    18481 aaagaagagg gtcaagcaat ggacgagttt gtccagcaat ctgctgtgaa agattctcgc
    18541 agatctgctc ataacgattg gtgattgggc ttttgttggg cttttttttg attttttgtg
    18601 aaacggcagt taaggaattt agaaaaaaat caggactcaa tcgaggcttt aaaagataat
    18661 cagcgacgcc ttctttaaaa gaatctttaa ccatatcaaa ttcatcatag ctagagagga
    18721 taaggatctg tgtttcaggt tgttgctgtt ttaatagccg cgataattcg attccattta
    18781 attcaggcat cattacatct gtgaccacga tatcgattgc atgtaccaaa gaaaaatcaa
    18841 gcgctttcag cggactggaa aaagtcccta ttaattgaaa cccgtgctca gaccaattga
    18901 tcaactgttt cattccatct aaaatcgtct gttcatcatc gataagaatt acgcgtattg
    18961 gcattttttt acccgcctta agaatagtct tgtaatttaa aaatacttat ttctaggaga
    19021 aaatgcaagg atatttttat tttaaagaaa cctcaccatt tcgatctttg gtctagatgc
    19081 taagattatt ccttttgtca tagttgattt gtattttttt attattttag gagaaatttt
    19141 tgaagtatct gaataataaa gaaaccgttt tcaaattttg gtatgatgaa tgtagcaaac
    19201 gtaaacaact aatggatgga aaaggagcgg ataaaaatga acatcagaaa gttaattgct
    19261 ggagtcgttt gtttagcggc agcatcaggt ttcttaacag gatgcggaag tgctagtagt
    19321 acagcagaca aagaagaaaa agtcactgtc tgggcgtggg atgagacatt caatatcaaa
    19381 gcagtgaatg aagcaaaaaa agtttatgag aacgaagaaa cagagataga agttgttacg
    19441 atgtctcagg atgatatcgt tcagaaactg aacaccgctt tagcgagcgg gaatacagaa
    19501 ggattgccca atatcgtctt gattgaagat tataggatac aaggctattt gacttcttat
    19561 ccagatgcat tcgctaattt gtctgctatt gtaaaggaag atcattttgc accgtataaa
    19621 tttgcggtaa acaaagtagg agataaaata tacggtgttc ctttcgattc gggcgtaaca
    19681 gcaaacttct atcgtacgga tctgatggca gaagcaggtt atacagaaga ggatatggag
    19741 aatctgaagt gggacgagta tattcaagta gcgcgagatg tcaaagaaaa gacgggaaag
    19801 aaaatcgctg aagtcaatcc ttctgacctt ggtcgagtaa ggatgattat gcaacaggca
    19861 ggagaatggt atacatctga agatggggag acagtcacga tccaagacaa tgcatcttta
    19921 aagtatggtc ttgaattgtt tgccactctt ttgaaagaag atctagtcga acaaacctct
    19981 gactggaatg ctggagtaac tgcgatacaa tcaggagcag tagcttctag cccaactggc
    20041 gcttggtatt cttcaacgat ccaaggagca gaaaaccaat ctggaaaatg gaagattgca
    20101 ccaatacctg cacttccaga aaatctgcaa aaagcgcaag cttctaacct tggcggcgct
    20161 ggctggtatg tgatcaaagg agtagctgga gaagattcag cgaaagattt cctagaagca
    20221 acgtttgctt cgaatgaaga actgatgggc actcttgcga aagaaatcgg actagtatct
    20281 acgatgctta gcgcgaatga acaagaagct tatcaagaag cttctgaatt ttacagtggt
    20341 caaaagatct ttgacgattt ttcaacatgg acatctgaaa taccagaagt gaattacgga
    20401 catgagacct atgcaattga agcagtcgta gctgaagctc ttcatcgaat catcaatgga
    20461 gaagagacag acaaggtact agcagataca caaaaacaag tagaagcaca attggctaat
    20521 tgatcggtgg taaaaaagtg aatttcttcg ctttttcatc agacaaacga aaagacaaaa
    20581 aagatcacgt ctgccttaca gatattgtct ttcttgtctt ttcatgtaga taatcaatga
    20641 tagaattgga gggaaaagaa tgaagcagaa agatttattc aaaaacgggc atgctttttt
    20701 aatcttgcca gtcattttga tatcaatcat ggtttttatt ccgatggttt tggctttgat
    20761 cacgtctttt caaagtggtc cccctgtcaa tatgacattt aacgggttag gtaattatca
    20821 gcggatgttg acagatgcaa cttttaaaaa agcttttttt aatacattcc tgtatttact
    20881 tgtacaagta ccgatcatgt tgtttctagc actttttatt tccaaccttt taaatgataa
    20941 aaagttgaaa tataaagggt tgtttcgaac cgctatcttt cttccatgta tcacttcgat
    21001 ggtcagttat tctctgatca tgaaaagtct attttctcaa tctggccttg taaacaactt
    21061 gttgttgggc attggtatga ttgacacacc gattggctgg ttgactcatc caatttgggc
    21121 gagagttctg atcattcttt cgatcacttg gcgttggact ggatacaata tgattttttt
    21181 cctttcaggc atgcaaaata ttgattcgga gatttatgaa gcagcatcta ttgacggtgc
    21241 ttctaaatgg cagcagttta tcaaagtgac gatcccaaat ttaaaaccaa tcattttgtt
    21301 tacttccatt acttcgacga tcgggacatt gcagcttttt gatgaagtac aaaatattac
    21361 tggcggggga ccagcaaatg caacgaccac cctatctcaa tacatctata atttgagtta
    21421 taaattcacg cctaattttg gatatgcggc agcagtttcc tttgttattg tttcctttat
    21481 tgtgatattg tcgataatcc aaacgaaagt aggaggagaa agtcgatgaa aaatatggta
    21541 gaaaaagaaa caatcgaacg ccccaacgtc aaaaaacaag cattagaact tcgttcgaca
    21601 attgggtcag tattaaagta tacagcatta agcatcctat catttatttc cattttccct
    21661 tttgtctgga tggttttggg aatgacgaat acacccgtgg atatcacatc gggaaaaata
    21721 aagattggca gtaacttgat catcaatttt caaaatttgt tttccaatga gctgaatttt
    21781 actcgttccc tttggaattc agcgatgatc gctttggtga ccactgtgtt tgcattgctg
    21841 atttcgtcaa tggcaggata tggatttgag atttatcaaa gtaaacgaaa agacctaatt
    21901 ttcaatattc tactgctatc gatgatggtt ccttttgcgg cattgatgat ccctttatat
    21961 cgaatgtttt cacaattgaa cggaacggtt ttgggaatca acagtttctt ggttgtcatc
    22021 ctgccatctg ttagcactgc ttttttgatc ttcttctttc gacaaaatgt taaatctttt
    22081 ccaaaagatc tagtagaagc cgctcgatta gacggtctaa aagaactaag tatttttttt
    22141 agagtgtata tgccgacggc taaaaatacc tatgccgcgg ctgcaatcat cacattcatg
    22201 agtagctgga acaattattt atggccctta gtagctttgc aatctcctga taaacggacg
    22261 gttccgcttg ttttatctgc tatgggtgct tcctatacac cagactatgg aatgatcatg
    22321 gctggattag tcatcgctac tttaccaact gccatcgtat tttttgtttt gcaaaagcaa
    22381 ttcgttcaag gaatgatcgg ctctgtaaaa taaaaaaaca caataagcgc gttccttcaa
    22441 agtcattttt ccaagtaaaa agatactttt gggacgtgtg ttattttatg ttgagaaaaa
    22501 ttaaatgata acgttttaag tttgtgctcg aaaactcttt cattttaagg taaacttaaa
    22561 gagtaaagga ggaggataca tgctaaaaaa ttttaaagaa gtgattgctg ctatattgcc
    22621 gatgactgtc ctgattgtca ttttaacgtt tgtttttgca ccgttagaag gagaagacct
    22681 cgcttcattt ctggttggtg cggcaatcat gatgatcgga atgacgctct ttttgtttgg
    22741 agcagattat tctatgatgg aagtcggaga tctagtaggg aaatatatga tcaaaaagaa
    22801 aagtttgacc gttttggtca gtttgggatt tgctatcggg atcgtgatca cgattgctga
    22861 accttccgtg caagttttgg gacaacaagt atatgatata tctgacggag agattggcag
    22921 agtgttgctg attgggatcg tcagcgtcgg cactggagta tttctagcct ttgcgctgct
    22981 tcgggtcgta ttcaagctat cctattatca attgatggct ataggctaca taggcgtttt
    23041 gatcgcctca tttttcacca gttccgaatt tatgccgatt gcttttgatt cggggggagt
    23101 gactactggt ccagtaactg ttccatttat cttggccctt gccggaggga tgactagtat
    23161 gatcaaacaa aagaaaaatg aaaatgacag ttttggaatg gtcggtattg cttcgttagg
    23221 gccgattctt gccgtgatga ttttgggggt gatcttccaa tgagtttctg gcagcaagtg
    23281 atagatggaa tcgaaatcgt taccatggaa gtcattttgg cagtggcacc tatcgtaatc
    23341 ctatttgttt taatgaatat cattagtttt cgtatgcgca aaaagcgttt ctttgacatt
    23401 atgaaaggtt tcttgataac gactattggg ttgatccttt tcctgcacgg agtcaatatg
    23461 gcttatgtac cggtaggaca gcatttaggc agtgctattg cgggattgcc ttacaattat
    23521 atactggttc cccttggctt tttaatgggg ttcttagtag ggtttgctga gccggcgatt
    23581 catgtaatga tcaaacaggt tgaagaattg agcgagggga gaatccgatc gaaaatcatg
    23641 ctgatggtaa tttcaattgg gatcggcgca gcagtaggat tgtctatgtg gcgtttgctc
    23701 gtcggattct ctctttatta tttcttgatc ccaggatacg ggctagcatt tattttagga
    23761 cgaaaagtgg acaagatctt tttagcaatg gcttttgata acggtggagt agcaacgggc
    23821 ccgatgtgtt cgacatttat tttatcaatg agcgtggcaa tcgctgcaca aattgaagga
    23881 agagatccga tcattgacgg gtttggtgtg gttgcaatga ttgcattgac accaatatta
    23941 tccactttag tactaggata tatttataaa cgaaaagatg aaaaagacag aatcgaaaaa
    24001 gaaagattgc aaaaattggg ggaaagcaaa tgaaagatct tgtagcattg aatcttcaca
    24061 tgattgtgac gatcgttgac ggaggaatgg gcgaagatgt tatcgacttt tcaaaagaag
    24121 caggtgcagc aggcggaacg atcttacatg gaagaggttc tggtgtacat gattcaggga
    24181 aatttttagg tttggacatt tcgccagaaa aagatattgt gttgacatta gtacccgata
    24241 gtttgacgaa taaagtaatg gatgcgattg gtcaaggcat caacatcgat attccaggta
    24301 atggtatttg ttttagtatt gatgtagaca aagtcattgg tatcactaag attcatcaat
    24361 atcgagaaat tatcggaatg caagaacttt tagatgaaaa taacgataga gaaaagtctg
    24421 aatcatagac aagcgtttga agaggaaata aacacaaaca agaaggcgga tgagcgttat
    24481 ttccgctttt ttgtttgtat ttttctcgtg aatacttatg aaattctact ctaagcatca
    24541 gactttcttt tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat
    24601 tttttagttg ttttaaaaga tactctgatt gagcgatcta catttctata tgttcaattg
    24661 caggaaaata aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta
    24721 ggagaaatct atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg
    24781 gaattaccag taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt
    24841 gaactgtttc cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca
    24901 gcagaactgc aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt
    24961 agacgtcagc aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg
    25021 atcaaaacga cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt
    25081 cctcaattga ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag
    25141 ctgtttcttc cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg
    25201 attgtgattg actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc
    25261 aattaaggct ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga
    25321 taatatgatg caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact
    25381 tatgcaggga agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct
    25441 tgtctttagt ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt
    25501 ctttttcgga tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga
    25561 gaatcaacgt tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa
    25621 caaaaagcaa gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca
    25681 tcaaaagcag cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg
    25741 tataaaattc gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat
    25801 ggcgaaagcg aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca
    25861 gaaaaaggaa aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa
    25921 ttcgatgccg tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta
    25981 gaagaaatgg gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat
    26041 tatggggatc ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa
    26101 gtaaaaacat ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt
    26161 gaccgcagat atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg
    26221 acagtgaatc ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg
    26281 caagatttac tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa
    26341 gatgttcctg aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat
    26401 acattagcag atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct
    26461 aaaagtgaga gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat
    26521 atatgggact ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg
    26581 caagcactaa aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa
    26641 aagtattcta ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa
    26701 ttatttcacc gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa
    26761 caagtactgg aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg
    26821 acagaatcaa catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta
    26881 gtagggatcg atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc
    26941 gatacttttc atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc
    27001 gaagcaggga aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt
    27061 gagatcccgg cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc
    27121 attaaaaagt agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata
    27181 aaattatatg gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag
    27241 attgccgaac caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac
    27301 gatcgaaaac tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt
    27361 gtttgataca tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa
    27421 aattgaaggt caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt
    27481 cgatatcgta cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt
    27541 aaaattgaat tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat
    27601 taaagctatc acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg
    27661 ataaaaatcg tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa
    27721 gaatcattct cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg
    27781 agaaaaatga caacactcat cacagtttta gtggttctag tttttgcggc attattatat
    27841 gtgttctatc gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta
    27901 ggagttggta tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc
    27961 gttactcaat caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa
    28021 atgctagtta tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt
    28081 tctgaaaagc tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct
    28141 attgcagcat tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc
    28201 acacaaggag ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt
    28261 gaaaacttat cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat
    28321 ttagcaggta gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga
    28381 atcgcttatc taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta
    28441 gtcgacagtc tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct
    28501 tatggagtat ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc
    28561 aatttaggta aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac
    28621 tcactgattt tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg
    28681 gtattgagct ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag
    28741 actcagacaa aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt
    28801 ttgtctattg gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc
    28861 gcaccagcag ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt
    28921 gtaacaatca gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg
    28981 atcgttttag gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa
    29041 ccattaatcg atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc
    29101 gtgacaagtg cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg
    29161 atcgatgtga atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata
    29221 agaagaagtt tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag
    29281 aagctacttc tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa
    29341 taagaacagc caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc
    29401 agatgcaagc ttttgaacac cgttttattt gtctcacacc cttttccttc ctacttgtaa
    29461 ccttgctccc gtgttagaaa catgttacac taaatatgtg aaattattaa caaggagcgt
    29521 cgttatgcga gaaaaaacag atacactgcc aaaagcaact gcgaaaagat tacctttata
    29581 tttacgctac ttgaagatgc tggatgactc aggtataagt agaatcaaat caaatgagtt
    29641 cagcgaaatc acccaaattc cttctgcaac gatccgcaga gacttctcgc aattaggcga
    29701 gctaggcaga agcggatatg gctatgatgt cccttttctt atcgatgtat tcaataatat
    29761 ccttaatact aaagaagaaa aacgaatcgc attagtagga tatggaaatc ttggcaaagc
    29821 attgaaacac aacaatttta gaagaaatga aaatctaaat atcgtgtgcg tctttgataa
    29881 tgatccagca ctgatcaatc gagtaattga tggagaaatg atctatccga ttgatcgttt
    29941 cgcggagata gccaaagcaa aaaatgtgac agtagcaatc tctacggtac caagtaaata
    30001 ttcacaatct gctattgatg aaattgtcaa aggaaatgta accgctatac ttaattttgc
    30061 gcctgacaga gtaactgtcc cagcttatgt caatgttcaa tacattgatt tgacaaccga
    30121 gttgcagacg cttatttact ttgatgagaa ttactcagaa gttttttctt aggaaaattt
    30181 gagtaggctt attgatcttg cgagaactcg tcgtaattct ttcaaaaatt ttttagtact
    30241 aaatgaagtg gttttttcac gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga
    30301 agcataacgc tgaacaagag gagtaactgg cacattcatt cttagagaga gaactatctg
    30361 ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg agtttctgtc ttgaatggag
    30421 tagagataga cgagtcgtga tcgttaactc acttgagagg caataatgat tgattattgc
    30481 aatttaggtg gtaccgcgta aatcatacgt cctagaatac gaaagtattt taggacgttt
    30541 tttagtttaa aggaggaaaa cagcatgacg gaagaaaata acctatcaac aaaatataat
    30601 ccgcaagaag ttgaagctgg acgttatcag cattggttag aacaagattt gtttaaaccg
    30661 agcggagata aaaaagcaaa accttattca atcgtaatcc cgcccccaaa tgttactgga
    30721 aaacttcatt tagggcatgc ttgggatacg accttgcaag atatgctgat tcgtcaaaag
    30781 agaatgcaag gatatgatac tttatggctg cctggtatgg accatgcagg aatcgcgaca
    30841 caagcaaaag tagaagcgaa gcttagagag caagggattt ctcgttatga tctaggtcgt
    30901 gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt atgcgagcca catccgcgaa
    30961 cagtgggcta aaatgggctt atctttagat tacagtaggg aacgttttac attagatgat
    31021 ggattatctg aagcagtacg taaagttttt gttaccttat acgaaaaagg tttgatttat
    31081 cgtggtgaat atatcatcaa ctgggatcct caagcacgaa cagctctatc tgatatcgaa
    31141 gttattcata aagatatcga aggcgccttt tatcatatga gctatgaact agcagatgga
    31201 tcaggcgtag tagaaattgc gacaacccgt ccagaaacga tgttgggaga tacggcgatt
    31261 gctgttcatc cagaagacga gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct
    31321 ttagtcaata aacaaatccc aatcattgca gatgaatatg tagatatgga attcgggaca
    31381 ggtgtagtaa aaatcactcc agcacatgat ccaaacgact ttgaggtagg taatcgtcat
    31441 gatcttccac gcgtaaatgt tatggatgat gatggtacga tgaatgaact agcaggaaaa
    31501 tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc
    31561 ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg gtcattccga gcggacaggt
    31621 gtcgttgttg aacctcggct gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa
    31681 aaagccattg ataaccaaga aacagatcaa gcagtggaat tcttccctcc aagattcaat
    31741 caaacgtttt tacgttggat ggaaaatgtc catgattggg taatctcacg acaattatgg
    31801 tggggacatc aaattcctgc atggtatcac aaagaaacag gtgaaatgta tgtagggatg
    31861 gaagcaccag cggatagtga aaactgggta caagatcctg atgttttaga cacttggttt
    31921 agttcagctt tgtggccttt ctcaacaatg ggctggccag atacggaaag cgaagattat
    31981 aagcgatact tccctactag cactttagta accggttatg acatcatttt cttctgggta
    32041 agcagaatga ttttccaaag cttggaattt actggcgaac gtccatttga gaatgtcttg
    32101 attcatggat tgattcgtga cgaacaaggg cggaagatga gtaaatcatt aggtaatgga
    32161 atcgatccga tggaagtaat cgaaaagtat ggagcagatg ctctccgctg gttcttatcg
    32221 aatggttctg caccaggaca agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg
    32281 aactttatca acaaaatctg gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg
    32341 accgtagaag agatcgattt atctggagaa aaatcagtgg ctgaccggtg gatccttact
    32401 cgtttgaatg aaacgatcga acgagtaact gagctatttg atcgttttga atttggtgaa
    32461 gcaggtcgac aactgtataa ctttatctgg gatgattttt gtgactggta catcgaaatg
    32521 agtaaagaag tactttatgg agatgatgct gtttcaaaac aaaccacccg tagcatctta
    32581 gtccatacac ttgatcagat cctacgttta ttgcatccaa tcatgccatt cgttacagaa
    32641 gaaatatggg aacatattcc tcatcaggga aattctctag tagtggcaga atatcctgtt
    32701 gtacgtccag agtttgatga tgaaacagct tctaaaggga tggaagtatt gaaagaattg
    32761 attcgagccg ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa accaattacg
    32821 ttgctaatta aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa tacaagctat
    32881 atcgaacgct tctgtaatcc cgaagagttg acgatatcaa gtgagatcac tgcgccagat
    32941 ctagcgatgt cagctgttct gacaggtgca gaaatcttct tgccgttagc aggattgatc
    33001 aatattgaag aagaaatcaa acgattagaa aaagaactgg aaaagtggac aagcgaagta
    33061 aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta
    33121 gtagaggcag agcgtgcaaa agaaaaagat tatttagaga agcaaacggc tgtcaaagaa
    33181 cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa agagagtgtg aaatcagcta
    33241 gctattacta aaagctagct gattgaacat cgttacagga aagagtttga gcctcactct
    33301 tagcttcgat tcataaggag atgtgacata aattttgtcg catctctttt ttagtctact
    33361 cttattatgt aggagaagga gggaggtgga tcatgtatca agagtcaata aagtagttga
    33421 taaaatcgtt cctctctctg tttcttttct ctttgtcata cttttatttt tcttatgtgt
    33481 tctatttgtt ataataaaag agaaatgggg gaattgttga tgacgattga agaagcaata
    33541 gaatggatcc atagtcgatt accatttggt tcacgtcctg gccttgaccg agtcgaagct
    33601 ttattagaag tggtagggca tccagagaga aaagtaaaaa cgatccatat cgctggaaca
    33661 aacggaaaag gttctactgt agcttatttg agaagcctat tagaagaggc tgggctgaca
    33721 gtaggtacat ttacttcacc ttatatcgaa acgttcaatg aacggatcgc aatcaatggc
    33781 aaaagcattc cagacaatga tttgatccag cttgtaaaaa aatatcagcc actgatcaaa
    33841 caacttgatc aatttgaagc agtagctggt attactgaat tcgagacatt aacagcaatg
    33901 gcatttgatt attttttgat gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc
    33961 ggcttattgg attctaccaa tgtagctgta ccaatgctga cgggaataac aacgattgga
    34021 cttgatcata ccgaaatact tggagataca attgaagaaa tcgctgcaca aaaagctgga
    34081 atcatcaaac aaggcgttcc agtagtaaca ggaaatattg ccccagaagc ttttactgtc
    34141 atccaacaaa cagctaaagc aaaaaatgca ccgctttatg tatacgggga aaattataaa
    34201 ataaactatc tgcatcctga tgagatgtgg ggagaagtat ttgaattttt taatgaaata
    34261 ggaaagatcc ctcaattaac cactccgctt ttagggcaac atcaagctga aaatgcagga
    34321 gtagccatcg aattgtattt tgtttactgt caattggaaa agctgccttt ccgagaaaaa
    34381 gatgttttga agggattgaa gaaatctcga tggccaggaa gaatggagcg tttgagtaac
    34441 gagccattag ttgttttaga tggtgcccat aatacgcatg caatgaaccg gcttgttgag
    34501 aatttgaaaa aagaattcaa aggatatcaa atcaacattt tgttttccgc cttacgaact
    34561 aaaaatgtta gagaaatgct agaacgattg cagcaagtac caaatgtcca tatttatttg
    34621 acgacctttg aacatccaaa agccatcgaa ttaagtgaag tatcagattt agcaagtgag
    34681 agagtatcaa tcgtttcatt gtggcaattc ggattagcag atatattgga aaagatgaca
    34741 aatgaagacg tattagttgt gaccggttca ctttatttca tttcagaagt tcgccattta
    34801 ttaatacaat taggaggaga tcatgaagta taatgcagtg atttttgaca tggatggttt
    34861 gctgttcgac acagagttag tgtattacga agccacacaa gtagtagcag acaagatggg
    34921 ttttccttat gataaagacc tgtatttaaa attcttaggt gtttcagatg aagaagtatg
    34981 ggcgaattat catatgattt tctcggaatt tggcaaaaca agtgtacaaa gattcataga
    35041 tgattcatac caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg
    35101 tgtgttggaa ctcctctctt ttttagaaga acatcagatt cccaaagttg tcgcttccag
    35161 taaccaacga gcagtcattg aacttttatt gaagaagaac aatctgtttg aacgatttga
    35221 gacgattgtt tctgcagaaa atgttcaacg agcaaaacca gacccagaaa ttttcttgtt
    35281 agctaatcag aagctaggta cagcgaagaa agaaacattg attttagaag attcacaaaa
    35341 tggtatttta gcagcttctg cggctgagat cccggtagtc atggtaccag atctgcttcc
    35401 accatcccaa gaattgatca aaaaaacagc tggggttgtt tcttctttac atgaaatccc
    35461 tgcattgtta aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg
    35521 agatgttttt ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga
    35581 acgtatggaa atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact
    35641 gcgtaccggt caacaccctt atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt
    35701 tggaggactg gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat
    35761 aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac
    35821 ggatcatgaa agagcggctc caccggttcg taccagctac gaattggccc agcaattgat
    35881 tctggaaatg aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa
    35941 tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc
    36001 tagagaaatt ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa
    36061 tcatccttct ggtaatgtta ttccatcgca gcaggatatg aattttacca aaaggataca
    36121 aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg gacgcaaacg
    36181 atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt tcttgcaaaa
    36241 aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag ttgaaacatc
    36301 tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt ttggcacgag
    36361 gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa aggatttgga
    36421 tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat ccaaggtgac
    36481 ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga atcagaccgc
    36541 ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa attgatggta
    36601 gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac aagagacaat
    36661 ttctcttgtg gcaggtattt ttttgtatga aaaagtaaat aaatttaaga ggctatgaaa
    36721 gagccacctt gtatcaagga ataagaacaa acaaaaatgg cttctcatgt ttttcatttg
    36781 ttttctctta tattcaggac aaggtgctga tttcataacc tcttatttat tcttcatctg
    36841 gtgtcattac cattggcaaa atcattggat gtctttctgt acgttcgaat aagaaaggct
    36901 gaagcgcgcc agtcatcgct tctttcaatt tatattctga acagtcaggt ccattcaaac
    36961 attctcttaa agcgtggaat aagattcgct gagcttcatt gatcatttct cctgattctc
    37021 gcatgtagac aaatccacga gagagaatat ctggaccagc caagatttct tttttcttga
    37081 tatccactgt cgcgacagct aatacaagac cttcttctga aagcaggtga cggtcacgta
    37141 aaacgacatt accaatatca ccgatcccgc taccatcgat atacacatct ccagccacaa
    37201 aatgcccagc aggacgcgca gaatcgctag ttaacgctaa aacatcgcca ttttccatga
    37261 tgaagcagtt ttctgcagga acacctacat cttgtgctag agaagcatgt actttcaaca
    37321 tgcggaattc tccgtggaca ggaataaaat attttggctt catcaaacga agcattaatt
    37381 tttgttcttc ttgtccaccg tgaccggaag tatggatgtt attgattttt ccgtgaatca
    37441 cttctgcacc agcttcagac agcaaattga tcaaatgatt gacacttgtt gtattaccag
    37501 gaataggtga acttgaaaag atcactgtat cgtctggctg gatttggatt tgacggtgag
    37561 taccattcgc gatgcggctc aacgcggcca tcgcttctcc ttgagaacct gtacatagga
    37621 tcatcgtttc attggcaggc aagcgattga tttcgtgtgc atctacaaat gtgccgtctg
    37681 gaactttgat atatcctaaa cgttgaccgt tgacgatggc attttccatg cttcgtccga
    37741 aaacagcgat tttacgacca gttgccacag cggcatctgc tgcttgctgc aaacggaaga
    37801 tgttagaagc aaagctggca aaaatgatac gtccttcgat ttttgaaaag atcttcaaaa
    37861 tagaatgccc aatcgttttt tctgatttag taaatgttgg aatctcagca tttgtactat
    37921 cagataaaag gcacaagact ccttcttccc cgatacgagc cattttatgc aagtttgctg
    37981 gttcgccaac aggagtgaaa tcgaatttga agtctccagt cgctacgata tttccagaag
    38041 gtgttttcac cacgatccct aatgcatctg ggatactatg ggtcgttctg aaaaagctga
    38101 cagaagtctt tctaaaacgg atgatcgtat cttcattgat ctcatggagc tctgcatctc
    38161 gaagcaagcc atgctcatct aatttgtttg tgatcaacgc taatgcgaga ggaccagcat
    38221 agataggaat atttgcttgt ttcagcaagt agggtattcc gccgatgtgg tcttcgtgcc
    38281 cgtgagtaat caccaaggct tttactttgt gtaagttttg tatgatgtag ctgtagtcgg
    38341 gaatgacata gtcaattcca agtaaatcgt cttccgggaa tttgatcccg gcatcaatca
    38401 aaatgatttc atcttggaat tgtaccccat acgtattttt cccgatttca cctaatccac
    38461 caatagcaaa gacgcctgtc tcgttatttt tgatggaaag tttcatatta aaactccact
    38521 aatgagaaat cagcattttc tttttcgtac tctagatgac ttccttctag aagttgaacg
    38581 aattcaatat tgtatggggt attttcttcc acacgttggc gagcgtctac atcgcttttt
    38641 gcctcaatgt aaagagattt tgtttcttcc cgtttagggt tgcgtacttt tgtttcttga
    38701 taataaactt tatagatcat tgttttgtct cctttatctg cgtaaagcag tgatttagct
    38761 agttgatttt tttagacaac acaaaacgag gggacaagcc acctggtttt tcatatgctt
    38821 tgactagaat actggattcg agtcaaagag acaagcctcg tttttgctta tctgttcgga
    38881 tattttttca accgtacctt ctgtataaaa aatatacaac aattaattgt tattctagca
    38941 taaaacagca aataagtata gaaatcaact agattgaaag aaaaaatcaa tgtttatcat
    39001 cccgctataa tcataatgac ttttttatga gagaagtgtt ctattaatag gctcgttgtg
    39061 ctatcatttt tagtaatgaa aatgtaacaa aagattaaaa ggagatgaga aagtgaaact
    39121 aatgtggcat tataccatgc gttataagaa attcttgttt ttcaatttca tatgcgtatt
    39181 tggttttatt ttgattgaat taggacttcc tacgatttta gcacgtatga tcgatgtagg
    39241 aatccgcaat gatgattttg attatgtgaa gcaacaaggg atatttatga tcgttattac
    39301 tgtaatcggt attattttga atatcttgtt aggttatttt ggttcgagga ttacgactaa
    39361 tatcgtagca gatattcgag atgatttgtt cgaaaagatc caaagttatt cgcatcagga
    39421 atacgaaaat ctaggcgtat cttcattgat cactcgtaca acaaacgatg cttatcagat
    39481 catgttgttc ctgcaaaata ttttacgtat cggttttatg gcaccaatga tgttcattgt
    39541 cagtttgtac atggtcatgc gtacaagtgt gacactcagt ttgtatgtag taggtgcatt
    39601 acctttacta ttattggcag tcgtagcgat tgctaaggtg tctgaaccgt tatctaaaaa
    39661 gcaacaaaaa aacttagata aaatcaacag tatcttgcgg gaaaatcttt caggattgcg
    39721 tgtgattcgt gcattcgtca atgaaaagtt tgaagaaaaa cgttttgctg tagtcaatga
    39781 gaattatgct tctagttcaa aaagcttatt ccgtttgatg gctgttgcac aaccgggttt
    39841 ttatttctta tttaatatcg taatggtatt gatcatctgg agcggggcag tgcaaatcga
    39901 tgcaggcgag cttcgtgtag gagatttgat tgcgtttatc gagtatatat tccatgcctt
    39961 gttctcattc atgttatttg ctagtgtgtt catgatgtat ccaagagcag ctgtttcagc
    40021 tggacgtatc caagaagccc tagatgccaa accttcgatt gtagaagatc caaatggtat
    40081 atctgataca aaaactaaag ggtatatcga atttcaaaat gtaacatttg cttatccagg
    40141 ccattcgcaa gagccagtga tacgaaatgt cagttttaca gcctctccag gagagaccgt
    40201 tgcttttatt ggaagtaccg gtagcggaaa atctacgttg atccaattga tccctcgctt
    40261 ttatgatgtg agtgaaggaa agatcttgat cgatggagta gacgtgagag attatcggct
    40321 aagcaagttg cgagataaga ttgggtttat tccgcaaaaa gcgctgctat ttactggaac
    40381 gattgcagat aatttgcgat acgggaaaga agatgctacg caagaagaaa tggaacgtgc
    40441 agctgagatt gctcaagctt cagaattcat ttctcgaaaa ccagatggat ttaatgaaca
    40501 tttatcagaa ggtggttcca atttttcagg tggacaaaaa caacgattgg caatagccag
    40561 agccatcata cgccgtccgg aaatctatat ttttgatgac agcttttctg cattagatta
    40621 tcaaacggat gcgaagttac gtgctcgtct gaaaaaagaa acgacagaat ctactgtatt
    40681 gatcgtagca caacgtgtgg gaacgatcat gcatgcagat aagatcatcg tattaaatga
    40741 aggagaagtc gtcggtatgg ggacgcatcg ggagttgttg gaaaaatgcc cgatttatta
    40801 tgatattgcc gcttctcaat tatcaaagga ggaattagca tgaaaaaaac cttttcttcc
    40861 ttaaaacgtt tagctcgata tatcagaccg tatcgtgaga cttttctttt agtgatccta
    40921 tttacagttc tgacagtggc tttcaacgtg gctttgccat atgttacagg acttcctact
    40981 acagaaatca gccgtaatat tgccaatggc gaatcaatca atttttctta tatattcaaa
    41041 tgtctcattt ggatcacagt tgttggaata gggtattgta tttcgcaatt actttctggt
    41101 gttttgatga caaatgtcgt tcaaagtgcc atgcatgatt tgagaagaga catagaagaa
    41161 aaaatcaatc gtcttccggt ttcatatttc gataaaaacc agcaaggaaa tattttgtct
    41221 cgtgtgacga atgatgtaga tgcagtcagc ggtgctttac aacaagcttt tattggtatc
    41281 gtaaatgcga tattgggaat tacgttggct gcagcaatga tgttttatat ccagccgatg
    41341 atggcgctga tctctatgat catgattcca acatcgatct ggatctcaaa acgagtgatc
    41401 aatgcttcgc agaaatattt tcaaagcatg cagaattctc ttggtgagct aaacggctac
    41461 gtccaagaaa atatgacagg ttttagtgta ttgaaagtat atggacgcga aaaagaaaca
    41521 ttggaaggat tcaaaaaagt taatcacagc ttgaaatact ttgggtttcg tgcagcattc
    41581 atttctggct tgatgatgcc tctagttcaa ttaactgcct atgctactta tattggtatg
    41641 gctgttctag gaagctttta tgttattact ggggcgatcg ttgtcggtca actacaagca
    41701 ttcatccaat atatttggca aatcagtcag ccaatgggga acgtgacaca attgtcttca
    41761 gttttgcaaa gtgcatcagc ggcaacgaaa cgtgtattcg agattttaga tgagccagaa
    41821 gaaaaagaaa acaaagtaga tgcttcactg ccagaaacga ttttaggaga tgtgacattc
    41881 gatcatgtcg attttgcata tgatccgaag aaaccattaa tccaagattt gaattttgaa
    41941 gtgaaagctg gtcaaaccgt agctgtggtc ggtccgaccg gtgccggcaa gacgacattg
    42001 atcaatctat tgatgcgttt ttatgacgta gacaaaggag caataaggat tgatggaatc
    42061 gacaccaaat caatgagtcg cagtgatgtg cgttcgctat ttggtatggt cttgcaagat
    42121 gcatggcttt atcaaggaac gattgcagat aatatccgct tcggaaagct tgatgcgaca
    42181 gattatgaag tagttgatgc tgcgaaaaca gcaaacgtag atcattttat ccgaacgatg
    42241 ccagatggat atgatatggc catcaattct gagggagaca atgtgtcttt aggacaaaaa
    42301 cagttgctga cgattgcccg agcagtggtc tccgatccta aaatcttgat tttagatgaa
    42361 gcaacaagtt cagtagacac ccgtttagaa gcgttgattc aaaaagcaat ggatcgcgtg
    42421 atggagggac ggacaagttt cgtcatcgct caccgtttat cgacaatccg tgatgcagat
    42481 ctgattctag tgatggatca aggtcagatc attgaaaaag gaacgcatga gagtttattg
    42541 gcacaaggtg gattttatga aaaactgtat aacagccaat ttgctgagga agcagaataa
    42601 gtcatataag aataaaacag tttaaaactt attttttgtt ctctattctt gataatggta
    42661 aagtaaagtt atcaagaaaa gtgaggacac ggagatggaa cagtttttaa atgaaaaaat
    42721 tactgaagcc aaaagagaag ttgatcgttt aacgcacgtc tgtcagacga agccttttga
    42781 ttcagctgat ttttttgaaa atgagtttca aatcgaacgc ttggcagcac aaatagaagt
    42841 gtacgaatta attttaaaaa aagtaataga agaaaatata gatgtacaat aaaaacaggt
    42901 aaaacgtcag ttaccagata ctgtaaatag acatctggcc tgagcaggtt ttacctgttt
    42961 tttttcttat gaaataaata tatgctatga tacctttatt caataggatt agtttctaca
    43021 aaaaatgaaa ggagaaaaat aaatatggaa cggtttatag catcaaatgc cacagtgatt
    43081 ggtgatgtga cattgtcaga agatgtgacg atctggtatc aagctgtttt acgcggggac
    43141 agtaattgga taaaaatagg acaaagaacg aatatccaag atggtacaat catccatgtc
    43201 gatcatgatg cacctgtaga tattgcggag aatgtgacgg taggacatca gtgcatgcta
    43261 catggctgta caatcgaaaa aggggcgctg atcgggatgg ggaccacgat attgaatcat
    43321 gcggttattg gtgagaatag tttgattggt gctggatcat tagtaacaga aggaaaagtt
    43381 attcctccta atgtcttggc ttttggtcgc ccagctaaag tgatccgtcc tttgacaaaa
    43441 gaagaaatac aaaaaaacaa agaaaacatc cagcattatg tcgagatagg tcggatgcat
    43501 gcgcaaggaa aattcaagga gcaaagatag gcagtagcat ccataaaata aaaaaggatg
    43561 gtcagtttga gttgaccatc cttttttatt tggtttagat aagagagctt aaaaaagctt
    43621 cctttgaaac agattaattt aatgaagaac tgttttccat gacttcatcg atcaatccat
    43681 atgcttttgc ttgttcagct gtcatatagt tgtcacgatc tgtatctttt tcaatcactt
    43741 caagcggttg tccagtgcgt tcagctaaaa ttttgtttaa gcgttcgcgt gtttgcaaaa
    43801 tatggcgtgc cgcaatttcg atttcagttg cttgcccttg agcgccgcca agaggttgat
    43861 ggatcatgat ctcagcattt ggtaaagcaa atcgtttacc ttttgtacca gctgtcagca
    43921 agaagcttcc cattgaagca gccataccca taacaattgt ttggacatct gctttgacaa
    43981 agttcattgt gtcgtaaata gccatacctg ctgtaactga accaccagga gaattgatat
    44041 aaagatagat atctttttca gaatcttgag cgtctaagaa aagcaattgg gcgatgatcg
    44101 aattagcaag atcatctgtt acttggccgc ttaacatgat gatacggtct ttcaataaac
    44161 gtgagtagat atcgtaagct ctttcacctc gtgaagattg ttcaatgact gtaggaatta
    44221 aattcataat agagttcctc cttgatttcg tttagaaata gtgtaccata atttagtttc
    44281 taatacctaa aatgtagtat acatttttgg tcaaaaaagg tcaaataaaa aacttaggca
    44341 aattagtcgc ataaacccgc tttatggtat gataagcaga gaaacaaaac aaggaggaac
    44401 aacagattga tagttggtat tactggcctt atcggagtgc tgacaggtgc tgtagttgtc
    44461 tatatcggca tgatactgca aatgcgtcac ttggaaaata aagaacagcg tagacgtgaa
    44521 ttggaattga aagtcaaaga aatcgagacg ctgaatcaat taaataaaaa gatcaacgag
    44581 atcttgcaaa aacggacaac gctgatggaa gaatatgtca gctttgatgc atttgatgat
    44641 tgttatatca cgattgacga ttttgcgtat ctccaatcat tttcagcgca aaatagtttt
    44701 tatctgccaa attatatttt agacgagcta tttaagaata tctcccatcg aaaagtgatt
    44761 ttatctccag atgaaacaat gaagattggt ggatatgctt ataaaggcgg acgcgtgatt
    44821 ttagagaatt ttacggatag tctgactgaa atgatcaatg aaaaaaaagc tcagttgaag
    44881 actatgacgc gccagccgat tggtttcttc tcgaaagatg atgtttggag ctgatatttt
    44941 gaagcattgc cgttgcacat gagagcgtgg taatgctttc tttttttctt aatgatacaa
    45001 tagaactgaa cggagtgaag aaagatgtta caaatctatg cccatcgcgg aagcagcggc
    45061 acccacccag aaaatacgct tcctgcattt gcagaagctg tacgagtagg tgcagatggt
    45121 atcgaactag atgtccatct aagcaaagat ggttatttga tcgttatgca cgacgaagaa
    45181 gtggaccgga cgactaacgg taaagggctg attcgagaaa aaacattgga agaactcaaa
    45241 aaattgaatg cagggggcaa attcagcaaa gaatttccag cagctaaagt tcctgaattg
    45301 agagaagtgt taggactgct cctccaaaaa aattaccgag ggatgctaat gattgaaatt
    45361 aaaactgatc agtatgaata tcccggtatc gaagaaaaat tagccgaaac attaaaaagt
    45421 agagattggc cttttcaaca tgcatattgc agctttcatt tggattcttt ggaaaaaatc
    45481 gcattactgg aaccggagat acagctggac ttattgatga gtacttctga taaaaagatc
    45541 caactagcag cagaaacccc ttatatagaa ggagtacatc caaaaaatac ttggttgttc
    45601 gaacatccag tccaggcagc tgattttcca aaagcaattc gtgcatggac gattaacgat
    45661 gaagaagaaa tggccaaatg tcaagaactc aaaatagatg gaataataac tgattttccg
    45721 gagaaagctc ttcggtggaa acaatcaacg aaaagtaggt aaaatatgga aaaagtatta
    45781 gtgatcgtcg gtccgacagc agtcggtaaa acagcgctaa gtgtggaact cgcacaacta
    45841 tttggaggag agatcatcag tggtgattca ttgcaaatct ataaaaatct ggatatcggt
    45901 acagcaaaaa tcaaagaaga agagatgaat aatgttccgc atcatttgat tgatgtgatc
    45961 gaacctactc aacgttactc agccgctgat tttcagcatt caggtcgaca attgattacc
    46021 gagattgcga accgagggca cctacctatc atagctggtg gtacgggtct ctatatccaa
    46081 tcccttctat atgattatca gttaggagcg acagaagaag cgaatcctga gattagaaaa
    46141 aagtatgaaa cgttagctga aacaattggt tcccaagcat tatggaatca gttgaaagaa
    46201 aaagacccgg cagctgctga gaaaatccac tggaacaatc aacgcaaagt gataagagct
    46261 ctggaagttt tagagacgac aggacatagt atcacggcac ctaaagaaga gccaaagcaa
    46321 ttgtatgatt acctcatgat tggattagat acgaagcgta gtattcttta tgaacgaatc
    46381 aatcaaagag tcgatttgat gctagcagaa ggtttagtag cagaagcgcg taaggtgtat
    46441 gagcttggag aagtccaggc cagtcaaggt attggatata aagaattttt cccttatttt
    46501 gaagggaaag aaacattgga agaagcaaca gaacaagtca aactacattc gagaagatac
    46561 gcgaaacgtc agttgacgtg gtttcgtaac cgtttggatg cacagtggtt tgatcttata
    46621 agtgagccaa atcaaaaaag tcagattgag caaacaatca aaaaatggct ggaggcggaa
    46681 taaaatggaa gaaaaagtga tcatcgtagg ggtagaaacc gaagcaaacc agcgttactt
    46741 ttcagagtca atggaagagc tagttcagct gacgaatacc gcatcaggag aagtcgtatt
    46801 cacaattaca caaaagagac cacaagtgga tcggcaaacg attatcggta aagggaaatt
    46861 acaagaatta gttcaacaag cggatgcgca tgaagcggat ttgatcatct ttaatcatga
    46921 gctaactccg agacagagcc aactgattac cgatgctgtt ggtcttccgg taattgatcg
    46981 cgtgcagctt atcttagata tttttgcgat gcgtgctcgt tcgaaagaag ggaagctgca
    47041 ggttgaactt gctcagttag aatacctgct gcctcgttta gtagggcaag ggaaaacatt
    47101 atctcgtctt ggcggtggga tcggaacaag agggccaggt gaaacaaagt tagaaacaga
    47161 ccgccgacat atacggaata aaatttttgc tgttaagaaa gaattgaaag aagtagaagc
    47221 tcatcgagag cgcaatcgcc aaaaaagaaa aaatagcgag atttttcaga ttggacttat
    47281 tggctacacg aatgccggga agtcaactat cttgaatcta ttgacacaag cggatactta
    47341 ttcgaaagat caattgtttg caacgttaga tccattgact aagagatggc gttttgctga
    47401 aggatttgag attactgtaa cggataccgt cgggttcatc caagacctgc ctacgcaact
    47461 gatcgatgct ttccattcta cattagaaga aagtcaaaat atggatcttt tgctacatgt
    47521 agtcgatgct agttctccag atcgtatttt acaggagcag actgtcttaa aattgatgga
    47581 tgaactgaat atgaaagaaa tgccgatact gactgtttac aataaggcag accagatcga
    47641 cccagcgatg ttcacgccta cgctattccc aaatgtattg atttctactc agacgaaaga
    47701 aggtaagatg gcgttgatcg aagcaatcaa acgtcagctg atggaactaa tgacccctta
    47761 tacactttct gtgccaagca acgaaggaca gaaattaagc gagttgagaa gacaaacaat
    47821 ggttttggac gaagcgtaca tggaagaaac aaacgaatat gaagttaaag ggtttgcaaa
    47881 aaccaattca aaatggttaa gaaaaaacca ataaaatttt atttttttaa
//
LOCUS       DACEOM010000013        44193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEOM010000013 DACEOM010000000
VERSION     DACEOM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.014"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            98..1123
                     /locus_tag="IW781_000716"
     CDS             98..1123
                     /locus_tag="IW781_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1181735.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            1162..2112
                     /gene="manA"
                     /locus_tag="IW781_000717"
     CDS             1162..2112
                     /gene="manA"
                     /locus_tag="IW781_000717"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1181736.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            2206..3909
                     /locus_tag="IW781_000718"
     CDS             2206..3909
                     /locus_tag="IW781_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1181737.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            3911..5944
                     /locus_tag="IW781_000719"
     CDS             3911..5944
                     /locus_tag="IW781_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1181738.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            5955..6395
                     /locus_tag="IW781_000720"
     CDS             5955..6395
                     /locus_tag="IW781_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1181739.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(6595..7722)
                     /locus_tag="IW781_000721"
     CDS             complement(6595..7722)
                     /locus_tag="IW781_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181740.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(7725..8402)
                     /locus_tag="IW781_000722"
     CDS             complement(7725..8402)
                     /locus_tag="IW781_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181741.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            8666..10432
                     /locus_tag="IW781_000723"
     CDS             8666..10432
                     /locus_tag="IW781_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1181742.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            10447..10884
                     /locus_tag="IW781_000724"
     CDS             10447..10884
                     /locus_tag="IW781_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1181743.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            10897..12048
                     /locus_tag="IW781_000725"
     CDS             10897..12048
                     /locus_tag="IW781_000725"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1181744.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(12110..12808)
                     /locus_tag="IW781_000726"
     CDS             complement(12110..12808)
                     /locus_tag="IW781_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1181745.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(12801..14153)
                     /locus_tag="IW781_000727"
     CDS             complement(12801..14153)
                     /locus_tag="IW781_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1181746.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(14376..15776)
                     /locus_tag="IW781_000728"
     CDS             complement(14376..15776)
                     /locus_tag="IW781_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1181747.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15937..16674
                     /locus_tag="IW781_000729"
     CDS             15937..16674
                     /locus_tag="IW781_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1181748.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            16799..17278
                     /locus_tag="IW781_000730"
     CDS             16799..17278
                     /locus_tag="IW781_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1181749.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            17275..18042
                     /locus_tag="IW781_000731"
     CDS             17275..18042
                     /locus_tag="IW781_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181750.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            18272..18841
                     /gene="lepB"
                     /locus_tag="IW781_000732"
     CDS             18272..18841
                     /gene="lepB"
                     /locus_tag="IW781_000732"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1181751.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(18945..19547)
                     /locus_tag="IW781_000733"
     CDS             complement(18945..19547)
                     /locus_tag="IW781_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1181752.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            19653..20122
                     /locus_tag="IW781_000734"
                     /pseudo
     CDS             19653..20122
                     /locus_tag="IW781_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            20228..20812
                     /locus_tag="IW781_000735"
     CDS             20228..20812
                     /locus_tag="IW781_000735"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1181753.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            20815..21888
                     /gene="prfA"
                     /locus_tag="IW781_000736"
     CDS             20815..21888
                     /gene="prfA"
                     /locus_tag="IW781_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1181754.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            21881..22720
                     /gene="prmC"
                     /locus_tag="IW781_000737"
     CDS             21881..22720
                     /gene="prmC"
                     /locus_tag="IW781_000737"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1181755.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            22713..22892
                     /locus_tag="IW781_000738"
     CDS             22713..22892
                     /locus_tag="IW781_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181756.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            22914..23939
                     /locus_tag="IW781_000739"
     CDS             22914..23939
                     /locus_tag="IW781_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1181757.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            24114..25358
                     /locus_tag="IW781_000740"
     CDS             24114..25358
                     /locus_tag="IW781_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1181758.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            25511..26458
                     /locus_tag="IW781_000741"
     CDS             25511..26458
                     /locus_tag="IW781_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1181759.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            26592..27221
                     /gene="upp"
                     /locus_tag="IW781_000742"
     CDS             26592..27221
                     /gene="upp"
                     /locus_tag="IW781_000742"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1181760.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(27360..27602)
                     /locus_tag="IW781_000743"
     CDS             complement(27360..27602)
                     /locus_tag="IW781_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181761.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            27723..27795
                     /locus_tag="IW781_000744"
     tRNA            27723..27795
                     /locus_tag="IW781_000744"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27756..27758,aa:Thr,seq:cgt)
     gene            complement(27914..28552)
                     /locus_tag="IW781_000745"
     CDS             complement(27914..28552)
                     /locus_tag="IW781_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1181762.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(28675..29307)
                     /locus_tag="IW781_000746"
     CDS             complement(28675..29307)
                     /locus_tag="IW781_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1181763.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            29658..29957
                     /locus_tag="IW781_000747"
     CDS             29658..29957
                     /locus_tag="IW781_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1181764.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            30452..32617
                     /gene="acm"
                     /locus_tag="IW781_000748"
     CDS             30452..32617
                     /gene="acm"
                     /locus_tag="IW781_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1181765.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(32889..33374)
                     /locus_tag="IW781_000749"
     CDS             complement(32889..33374)
                     /locus_tag="IW781_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1181766.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            33854..34495
                     /locus_tag="IW781_000750"
     CDS             33854..34495
                     /locus_tag="IW781_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1181767.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            34514..35149
                     /locus_tag="IW781_000751"
     CDS             34514..35149
                     /locus_tag="IW781_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181768.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            35167..35982
                     /locus_tag="IW781_000752"
     CDS             35167..35982
                     /locus_tag="IW781_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1181769.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            36301..37737
                     /locus_tag="IW781_000753"
     CDS             36301..37737
                     /locus_tag="IW781_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1181770.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            37926..40373
                     /locus_tag="IW781_000754"
     CDS             37926..40373
                     /locus_tag="IW781_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1181771.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            40603..41670
                     /gene="rlmN"
                     /locus_tag="IW781_000755"
     CDS             40603..41670
                     /gene="rlmN"
                     /locus_tag="IW781_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1181772.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(41863..42966)
                     /locus_tag="IW781_000756"
     CDS             complement(41863..42966)
                     /locus_tag="IW781_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1181773.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            43519..44067
                     /gene="aac(6')"
                     /locus_tag="IW781_000757"
     CDS             43519..44067
                     /gene="aac(6')"
                     /locus_tag="IW781_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1181774.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        15097 a         7596 c         9020 g        12480 t
ORIGIN      
        1 ttttcgcgca gtttttttgt gtaaaaaagg ttgattcctc atagattaag ctttaagctt
       61 ggactgtaag aatctgatag aaaagcagga gaaaaagatg tatccacaca cgaaacagct
      121 tattttgaaa aagatggaag aaggatttta tccaggtgta gcagtcgcct tcataaaaga
      181 aaatcaagta gaaacattta cagccggctt tgcccaagtc gttcctgaaa agcagctgat
      241 gacagaagat ctgttgtttg atgcagcctc actgacgaaa gtcgttgcaa ccacttcatt
      301 agttttacgt ctattagaag atggaaagat agaactcgat caaccgcttc ataaatatct
      361 tcctgaattt gaaaatcaga caatcacttt gcgtcattta ctgacacata cttctgatat
      421 caaaacatgg ataccaaaca gagataagct atctcaggaa gaattgatcg ccgcttattt
      481 gaagctagaa ccaggtgagc aactaggaaa agaagtaaaa tatacagatg ccggtacgat
      541 tcttcttggg ttgatgctag aggaaattta ccaagaaact gcagtggaaa tctttcaaga
      601 acaagtattg gaaccattag gaatgatgaa cagcttattt cttccttttc cttccaaaaa
      661 aatcgttccc acagaacaac tgccttcggg tgaggttttg cgtggacaaa ctcatgatcc
      721 gaaagcaaga gttttggctg aacatgcagg taacgcgggt ctctttattg atttgaaaga
      781 cagcattact tttgtccaaa tgtatttgaa caagggaaaa atccgtaatg gtcgtttttt
      841 agaagaagag acgattgatt ctttactgca agatcaaaca ccgaaaaaaa atggctatcg
      901 ttctttagga tgggacttaa aaaaggatgt agaagacggt catgcgattc tttttcatac
      961 tggttataca ggtacgtttt tcttgattga tataaaaaca caagaagcgt tgatattttt
     1021 gtcaaatcgc gtacatcctg tcgatcatcg tgcggagtat ctcatatgtc gagatgaaat
     1081 tgttgaaaac tttttgaaag agaaagcggt attaccgaaa taatgctata atagctatga
     1141 aaaatgagag gagtttgaat tatgcaggaa cctatgtttt taaaaccagt ttttcaagaa
     1201 aaaatttggg gaggaagtcg tttacgttca gtctttggtt ttgatatccc taacgataaa
     1261 atcggagaag attgggcgat cagtgcgcat cctcatggtg tcagtgtagt agaaaatggc
     1321 gaattcaaag ggaaaagatt agatgaacta tggtcagaac acaaggaatt gtttggccat
     1381 ccttcagaac cagtgtttcc attattgatc aagatcttag acgcagaaga tgatctatct
     1441 gtacaagtac atccagatga tgcctatggg atgaaacatg aaggtgaatt aggaaaaact
     1501 gaatgctggt atatcatcga tgctgaacct ggtgcagaaa tcatctatgg tcatcatgca
     1561 aagacaagag aagaactagc tgagatgatc aaagatgggc gctgggatga ccttttgaag
     1621 aaagtaccag ttaaaaaagg tgatttcttt tatgtaccaa gcggaacgat ccatgctatt
     1681 ggtaaaggaa tcatgatttt agaaacacag caaagttctg acacgactta ccgcgtgtat
     1741 gactacgaca gaaaagatgc gcaaggacaa acaagagagc tgcatatcca acaatcgatc
     1801 gatgtaacaa ctgtccctgc gaaaacacca gaattacaaa tcaaagaagt ccgaaaaggg
     1861 aattcatcga tcgtcactta tttagaaact gagtttttca atgtatatga gtgggatatt
     1921 aaaggaatca catctttcaa aaaacaagcc ccttatacat tgatgacggt aatcgaagga
     1981 gcaggagacc ttgtcgttga cggaaaaaca tatccattag aaaaaggaac aagctgtatt
     2041 attccaaatg gcgtttctga gtgggcggtc caaggtgaac tagcaatcat tgcgtctgaa
     2101 ccaggcaaat agttcccaga tagctgaaag cagcagacgg tttttcgttt gctgcttttt
     2161 ctacataaaa tccgtccatc aataaataga agtctggtga aaaagatgga atacaaatgg
     2221 aaagcaaaca acagcagtcg cgtgtctatg aaacaactga gaaaagctgg acatcactta
     2281 aatgaaatga tcatgccaaa tctaagtctg tttatcgctt ggggaatctt gtcattagca
     2341 acaaaaggtg tgtctggcga tttgcacacc acattagaag aggtatgcca ttggatgatc
     2401 cagctgcttt tacccatctt gatcagttat tcaggcggga aatcggtagg gggacaaaaa
     2461 ggggcagtcg ccggtgcaat tgcttcttta gggttgatcg ctcgttccaa tgctccacaa
     2521 atcgtaggag cgatgattat cgggccgctt gccggcatcg tatatgaaca atttgtttct
     2581 agattccatg tgaaattcaa ggctggatat gaaatgcttg tcagcaacct actagtagga
     2641 ctgactggta gcgttatttg tataattgga atcgttttta ttgaaccagt tttaaatagt
     2701 gttcacttgt tgctcgcctc cgttgtgtct tggctagtca caatgaatct gttaccactt
     2761 gtcagtctga tcttagaacc tctaaaagta ctatttttca ataatgcaat caatcatggt
     2821 gtattgacac ctttaggaat cgaattctca caaacagtcg ggtactcttc cttatttttg
     2881 atggaggcaa atcctggacc tggtttaggg atcttacttt ctatcctttg ttttgcagaa
     2941 aaacaggaaa aagtaaatgc gagtggagca ttgatgatcc atgcgattgg cgggattcac
     3001 gaaatctatt ttccatttgt cttgctgcga ccgtatctat ttttagctgt aatggctggt
     3061 ggggcaagcg gcacgttgat atttcaatgg ttagatacgg gattagctgc acctgtatcg
     3121 cctgggtcac tactcgtgat ttttgccaat gcgccttatc cacaactagc tggggtaagt
     3181 atagggatag caatcagtac atttgtgacc ttcatttgtg cagcgatact attgaaatta
     3241 ccaaataaag aaaaagagaa caaaacggtc aaagaaaggg gagaaatctt ggaaccatct
     3301 atcattcatt ccgtcatttt tgcttgcgac tcaggtatgg gatcaagtgc tatgggagcc
     3361 gccctgctga aaaagaaatt agcagcagaa aaaatcgatc tttctgtcgg atacagctct
     3421 atctatgaat tgaataacca agtaaatcag ctggttatcg tgcaacatga actagcgggt
     3481 cttgcaagag aaaaagtacc tcaaagtcaa atctatgtac tggatcactt tttggaaaca
     3541 gatatcttgc tgtcacgaat aaaagaagcg atccaatttc caaagcggga agaaaaaaaa
     3601 gagatagcag atatcacaga acaaaaaacg tcgcctgcta atattgtatt tttgtataaa
     3661 aacaacagac gcggctcaca aactatgggc atgacgattt taagtcaatt agcaaaaaaa
     3721 gcgaaaaaac agtttgttat ctcaaaagaa tcaatagaaa aaatcaaaat ccaagaagaa
     3781 acgatttata tcgcacaaag agaactagct gtaactgaac agctagaaga aaagatccct
     3841 cggctagttc tagttgaaca ttgggtagct acgaatgaat acgaaaaact cgtgaaggga
     3901 gaaatataag atgtatttat cgcccagaga aaaacaatta ttagcagaat ttctcagtag
     3961 cccgtcacct gtatctatcc aaaaaatgat gaatttattg aaggtcagta aacggactat
     4021 ttaccgcgaa ttagataatc ttgaagtctc attgaaatcg gtaaatgctt ctttgaaaaa
     4081 agcagctaga ggctcatttg tcattgaggc atcagaagaa gtattagagc aattaaaaga
     4141 aaatacagat gggaaaagta atgacttatc cactgctaaa cgacaacacg cgattttagc
     4201 cgagttgtta ctgacgacag agccgttgag tcttgctcat ttcatggaat catatcgcat
     4261 cagtaatacc actttttatg cagatatcaa acagttggaa gaaagtattc gccaactgcc
     4321 tttggaaatc attcggaacc agggatacga gatcgaagga ccggaaaaat accgccggct
     4381 tctgacagcg aatgtattag aattagaaat caatgaatat gaactttttc atagtatttc
     4441 tttcgatgca tcactgaatt atttttttcg attcgttgat ccgcaacatc tatctcttgc
     4501 acgcaaagta atcggggaag aattgatcca aaaaaagacc aatctaagtg atagaaagct
     4561 tgaacatttg gttttgatgc tgacattgac gatggagcgt gtgacgaaaa atcatttact
     4621 gacaaatgag acatataata gtctggcaaa caaagaatta ctacagacag ctaaacgatt
     4681 gttttcacgg atcgcttctg agacaaaaca actttatcca gtaaatgaga tcgtcttttt
     4741 tgcaggatta ttaaatgact tcacgaactc atttgatcag aatttctttg aagagacgtt
     4801 cgatacacag cttgcttatt tagtaaaaca actgattcaa gaagtaagtg aagagacgaa
     4861 tgtccatttt tatgaagatg aaactttgta caaaatgctg ttgactcatt tatctggtgt
     4921 gttttcaaga gccgttttac aagaagagac actgaccaat ccgattttag aaaaaatcat
     4981 ggttcaatac gaggaagttg cgaatgcaat ccgtaaagca gtagagcgga tttttcaaga
     5041 aaaaaaatta tccgaagaag agatcgctta tatggtcctt cattttgcga attcattgga
     5101 acgaagtccg aaagaaagca atatcagtat tgcaggattt tcgccaagtg gattagcttc
     5161 tactagcatg ttggaactac gattgaaaag gtattttccc ttcatccaac aaattcattt
     5221 ctatcggatt gctgatttaa aaaaaatcga tttacaagaa gaatatgatg ttgttatttc
     5281 tacatctatt ttaccaggat acggtggaaa atatatttta gtctccccgc tactgctaga
     5341 agatgaagtc aagcagctaa aagaagcttt ttcgcatatt gagaagaaaa aaaggcaaaa
     5401 aattacgtat aacagcaata acgagtggat gatagaagat acttatgagg aaacaatgca
     5461 tttcatggat caaatcaatc aattactcgg tcgttttttt atccaagaac tggataatcc
     5521 tgagacgatt tccgaaacga ttcgcagctt atttccttat tttcctgaag gactagtaac
     5581 ggatgaagag gtggttcatc gccggctgct taagcgatat gaacaagcac ctatcggtat
     5641 tcctcataca aatatggggt tattccatac atccagctca ttgatttcta aaccgatctt
     5701 ttgtattttc aatttgaaac atccgttgac cattgaaggg atggatagac agccagtcga
     5761 tttgtcccgt atgttagtta tgctggcgcc ttcgccaatc caagaaaaag acgggattct
     5821 tttaggaaag atcagtggag caatcatcat gaatgacttg aacaccgaga tcttccgctc
     5881 aggaaataaa gaaatcgttt atcagttgtt agcgaaaatc ctgattgaag aaataaagaa
     5941 atgaaggtga aaagatgata cagctaccag cagaaaaaat attgctcgat caatcctatc
     6001 aaaattggaa agaagcgttg accgagatcg gccagtggat gacccaacaa gggggaatca
     6061 aaccggcata tatccaatca atgatcgaaa gacaagaaaa agcgagtgtc tatatcggga
     6121 atttcgtagc cttgccacat gggactgaca aagatgaaga agtagtagaa gaaggaatct
     6181 atttagtcca agtgcctgat ggtgtggact ttggaacaaa agaggaacca aaaatcgcga
     6241 cactcttatt tgtagtggct ttaaaaaaag aacatcagtt agcggtgctc caagatttag
     6301 cctttttctg ttcagacatc gaccaagtaa tggcgctcag tgatgcggaa accattgaag
     6361 aagtccaaat gatttttgaa catggacata tataaccaat gttcacagta aaaaaagctg
     6421 ccttgcatca agtaataact tcaaccaaga taaaaataag aggagctatt tttgttggga
     6481 ggttcttatt actgcggatg caagcttttg aaaactattt attctagaaa accgtttatt
     6541 tgaaataggg gacggtgaca aggtttttgt caccgcccct attatttttg tgttttaata
     6601 gctctttttt atatagatag tgcccatacc tatgaataat aaaacgatac tccatatacc
     6661 aagtgtcaag ataccattca tgaaagtaac ctgttctgaa gctagtggcg agaaaatatc
     6721 tcctcctatg atcacatttt gaaaatcaac ataagaagtt ggcaagtaat gactgattga
     6781 ttctatccat ccaatcgaag aaaacaagcg atactctgct aagaaaagac aaagaagtgt
     6841 acctaaaaga ttgactagag aatgttcaaa taaagcagat agaagaaaac tgattgtact
     6901 taaaaagagc acccacaaca acaggaacag aaagctctgg aaaagatact catacatcgg
     6961 caggattcgt atctcctgac caatgatcgt tcctacagga taacggaagt ctcccaaacc
     7021 atttttgatt attactagta tagagacgat caaaaatggc atcacgagac ttagcatagc
     7081 tgcaccataa taaatggtaa aacggatgct atgtttcttt accatactat ccggtaatag
     7141 attgactaga gagattacct gctttctttt ttcataagag atgatggaag aaagaaacaa
     7201 ggcggttata cataaaatca aaaaactaga aatcattcct gaaaaaatat tttcgtagta
     7261 atttgctaaa gagagatgcg ctggcaagtc aaaaatagaa tatctttgga tctgatgttc
     7321 tttcatataa cggagcaact cgacacgttt ttgttgttca atgattggaa ttccttgtct
     7381 ttgaccagaa atcagtcgat ccagtatatc tttttcataa tgataagttg cttcgaccat
     7441 ttgctggaca ttccctgtgt tttttgcatg taagattgct tcgatcagtt tgttgctttt
     7501 ctctaaactt tccacaaatt ctttctcatt ctcatcttct ttcaacgttt caatcaaacg
     7561 ttgcatctct tcatagttat gattcagttc atcgaattct gtataagcaa gtttatgatc
     7621 aaaaaaaagt gggaatgcaa agagcacgac caacaataga ggaaataaaa agtttacttt
     7681 gttttttagt aagagattgc gttcaaatgt aagtaaagac atagttacac ctcatataat
     7741 ttacgataaa tagctaaaat ctcttccata tttgaggatt ctgtgacgat ttctccattt
     7801 tttagaaaac atgctttaga acaaatcgat tggatattcg cgatagcgtg ggaagaaaga
     7861 agaatcgtct tcccctcttt ctgccattgt ctgatgaggc tattcaagat atcaatactt
     7921 cctgggtcta atccgttcaa cggttcatcg aataacatga cagatgaatc actgatcaag
     7981 taagcagcaa gcaagacatg ttgtttcatt cctagagaca tttttccaat cggcaaagta
     8041 aggtactcag tcatcttcaa tcgttggaaa acagtttggc attctacttt gctctgccaa
     8101 tagtatttga tcaaagtcaa ataatcaaca gccgatagat agtcggataa ctgattcgtt
     8161 gattctagaa aaaagaaaga ccggcgataa tctgtataag cagtaggaga gatgttgtga
     8221 agggacaatt gatcataagt tgttggcagc agttgggcaa tgccattcag caaagttgtt
     8281 ttgccaaatc cgttcggggc aatcaaagca gctacttctc ctgattctac taaaagattc
     8341 aattgtcgaa tcacacgttt tttaccacgt ttcatgttca tgtttttgat cagtaatgtc
     8401 atagataggc ctccaatata aaatttaata acataataga atggaaaaca tctaaattaa
     8461 atgctaacaa aagttattgt ttgcatatta taaaaaaatt aaaataacag ttttatttaa
     8521 aattgaagtt ttctgatacc gaaatctttt tattttgctg acatactcaa acctttggca
     8581 caaagtttcg tgccaaagta cgactatttc tctttttagt aagcggatac aattagagta
     8641 tcaaaaggag agagggagag acaaaatgga acggacgatt cccaaaaaca acatttcgct
     8701 aaaagcgagg gtacaaaaat taggcagtac tttatcaagc atggtcatgc caaatattgg
     8761 agcactgatt gcctggggtg tattaacggc tctatttatc ccagatggtt atttaccaaa
     8821 tgaggctttg gctaccatgg taagcccaat gctaacttat ctgattcctt tattgatcgg
     8881 ttatactggc ggaaaagtaa ttgctggtga ccgcggatca gttgttgggg caattgcaac
     8941 gatgggtgtg attgtcggaa cagatattcc aatgatgcta ggcgcgatga tcatgggacc
     9001 tcttggtgga tatgccatca agaaatttga tcagctattc caaaaacgga tcaaatccgg
     9061 ttttgaaatg ctagtcaata atttttcagc aggtcttatc ggttttggat tagcgttatt
     9121 aggtttttct gcgattggtc cagttgtcga tgcattgact caagcaatgg caaaaggtgt
     9181 agagatcatt ttatctgccc atttgattcc attgacaagt atttttattg aaccagcaaa
     9241 aatcttattt ttgaataatg caatcaatca tgggattttg acaccattgg gaactgaaca
     9301 agtagcagag gctggaaaat ctattctatt cttgcttgaa gcaaatcctg gtccaggact
     9361 tggcgtatta cttgctttta ctttgtttgg caaaggagca gcaaaatctt cagcaccagg
     9421 agccatgatc attcactttt taggcgggat ccatgaaatc tattttcctt acgttatgat
     9481 gaaaccgtta ttattcttat cagttatagg cggaggaatg tcaggaagtt tcattttcca
     9541 attgatggat gctggactaa gagcgccagc ttctccaggg tcgatcattg cgattctagc
     9601 gatgacaccg ttcaattctt accttcctgt tattttgggc gtgattgttg ccgcagctgt
     9661 ttcgtttggt atttctgcag taattttgaa agcggatgcg aaagatacaa atgaagactt
     9721 cgaaaaatct gttcaagaga caaaggcagc taagcaagct tccaaaggtg cccaaggtat
     9781 tcctcaagga gctacattct ctggcgtaca aaaaatcatt tttgcttgtg atgcaggaat
     9841 gggatcaagt gcgatgggcg cttcgatctt gagaaaaaaa gtacaggaag cagacttgcc
     9901 tcaaacggtg accaatcaag caatcagcca actgacagat gactcgaata cattgatcgt
     9961 tactcaggtc gaactacaag aaagagcaaa acaaaaagca ccgagcgcgc gttttgtctc
    10021 agtagaaaac tttttgaatt cacctcgata tgatgaaatc attcaagaat taagcagcaa
    10081 gcagaaaaca tcagtttctg aaattgagaa tgagccagca gaagagtttc tttcagcaaa
    10141 catcaaacaa atcaaagaga ttctacttgt ccacgatgat cgttccggat cagcaacaat
    10201 ggggatgacc gtgctcaaag atatattaga caagaaccat ttaactatcc cgttaagaaa
    10261 agtcaatatc caagatttaa aagatgaacc tgattcactg atcgtagcca aagaaagttt
    10321 agcggaaaaa gtacagatgg ctgcacctca atccattcat ttatcagtca acagcatggt
    10381 caatccgcaa aaatacgaat cgattgcaca atatttgaaa cagacagctt aagggaggaa
    10441 aacagcatgc aaagtttgcc attcgacatg atcgagctca atcgatcttt taaaaccaaa
    10501 gaagaagcga tccgctattg cggtcagatg cttgtagaag cagggtgcgt tgatgaggaa
    10561 tatatcgatg ccatggtaga tagagacaaa atgttatctg tttatatggg gaattttatc
    10621 gcgattcctc atggaacaga tgaagcaaaa aaatatgtga aaaaatccgg catctgtgtc
    10681 gttcaagtac cagatggtgt agattttggt accgaagaag aagaaaagat tgcgactgtt
    10741 ttgttcggta ttgcaggagt cggagatgag catttgcaac ttgtacagca aatcgcactt
    10801 tattgcagtg atatggacaa tgttgtccag ctagcagacg cgctcagcaa agaagaagta
    10861 acggaaaatc tagcaattgc ttaaggagag agaaaaatga gagcagtaca ttttggtgca
    10921 ggaaatatcg gtcgaggatt tattggagag gttttggcag ctaatggatt tgagattact
    10981 ttcgtagatg tgaatcacac gatcattgat gctttaaatg aaagaaaaga atacgaaatc
    11041 gaattagcag atgaatcaaa agaaaaaatc actgtaaaaa atgtcaaagg aatcaacaat
    11101 caaacaaatc cagaaaaagt aatccaagaa atcgctgaag cagatttagt tactaccgcc
    11161 attggcccga atatcttacc ttttattgct gagttgattg caaaaggtat ccaagaaaga
    11221 gaaaaggaag gaagcacaac atctttagat atcattgcct gtgaaaatat gattggcggt
    11281 tcagcatttc tagaaaaaga agtgtataaa tatcttccgg aaacatcatt tactgacaaa
    11341 tatatcggct ttccaaatgc agcagtcgat cgaatcgtcc cattacaaca ccatgaagat
    11401 cctttattcg tccaagtgga acctttcaaa gaatgggtca ttgacgatag ccaaagaaaa
    11461 aataaaaaaa tccaattaaa aggggtttta tatgtagatg atctagaacc atatatcgaa
    11521 cgcaaactat ttagtgtcaa tactggtcat gcgactgtcg cttatacagg tgccttgtta
    11581 ggttaccaga ccattgatga agcaatgcaa gatgcgttag ttgtggcaca attaaaatca
    11641 gttttgcagg aaacaggaag tttgctgatt gccaaatggg ggtttgatgc agagcagcat
    11701 catgcgtata ttgaaaaaat cattcatcgc ttccaaaaca aaaatatctc tgatgcgatc
    11761 acacgtgtcg ctcggacacc attacgtaag ttaggttatc aagaaagatt tacacgtcca
    11821 gtgagagaac tacaagaaca taacttgact tgcccacatt tgacggcaac aatggggatt
    11881 atttttaatt actatgatcc tgaagatgaa caaagccgtc aattgcatga aatgaagatc
    11941 catgaaaact tggaacaatt gattcaagaa gtcacaggaa tcaatgatcc gaaaacaatc
    12001 gggaatatca agcaaaacgt gaaccgttat gccaagcaag tcgcttaaaa taggatacga
    12061 aaaaggggaa tgtgacgaaa tttttgtcac attccccttt ttcgaataac taggctgttt
    12121 gtccttcaag atagggcaga tccaattctt taccgtaacg ttgttctaaa gctaggcgga
    12181 tcaaacgagc tgccaggttc tcatttcttg caaggatagg tccatggaaa taagaaccga
    12241 agacatttcg gtaaatgaca ccttcagatt ggtcttcacc attatttcct tgaccttgta
    12301 ccactttccc caaaggtcgt tcaccttcac ctaagaaagt ccgaccattg tggttttcaa
    12361 atccataata agtttcattg aattcttcgt tatggataac gatatcaccg ataaaccggt
    12421 tattttcctg acttaacgtg tagtgatcca gtgcactgat tccttggatc ttttctccat
    12481 tagctcccat gtagtagtga cctagcaatt ggtagccacc acaaatggct aacatgacgc
    12541 catcattttc gatatagttg atcaaagaat ctttcttttt ctggatgtct tttgaaatga
    12601 ttaattgctc aaagtcttga ccaccgccaa agaaaaccaa gtcatattta tcagcatcaa
    12661 aaggttcatg tatactaacg atctccgtat ggcaagaaac ccccattttt tctgctatat
    12721 atttcaacat caaaaggttt ccgttatcac catatgtgtt catcaaattt ccataaaggt
    12781 gggcgatatt gagttcataa ttagccacgg ttcattcctc ctttgataaa tccttgagca
    12841 gctagttctt tacgaagctg caatacagca gtgtaagtag ctaaaatata gacatgctca
    12901 gttggcaatt ctttgatttt ttcgattact tctggtaatt cttttgtttc tgtcaattta
    12961 ttttctggaa tcccggcaac ttttagacgt aaagtcatat ctgtatgacg gtctcctcca
    13021 gaaataacag ctgggatatc catatcggca aagttttcaa aattaccatc ccaaatccag
    13081 ctgacatcga ttccgtctgc gtaattggca tttaataaag ccactaaaga aaatggataa
    13141 ggagctagac caatcatatc gattacttga ttgataccta caggattttt gacaagtact
    13201 aatgtacatt ttttcccatt gatattgatg acttcttgtc ggccgaatac tttctcgtca
    13261 tatcctaaac cttgtttgat tttatcagga tcgacattga agtactctgc cactgcagta
    13321 gctgctaaag cattgtagac attatacata ccgccaactt cgattccgta ttcatgacca
    13381 tcgatcacga aatctgctga cacattgtcc atccgtacca tatcagttaa tcgataatcc
    13441 aattcaggac gtttgaagtc acaatttgga cagtaatatt tccctaagtt tgcataagtg
    13501 atcattttat aatgcaagat gtgatgacaa tttggacata agacaccgtc tgtattgtaa
    13561 tgagccattt gttcacgatc ttcttcatgg tcaaagccat aatattttct aggattgact
    13621 gtttcgatag aattgaagat tggtgaatca ccgttactga taatcgttgc atttggtgac
    13681 tttgcggcac cgtctacaat cattttatag gtcgtataga tttctccata ccgatccatt
    13741 tgatcacgga aaatatttgt aaataagaac agttctggtt tcatatattc tgtgactttg
    13801 ctcaaacttg cttcatctat ttctaggaca gcaaactttt tctgtccttt ttttggtttt
    13861 gcctgcaaga aagtcgatac aattccctgt accatattcg caccagtagg gttggttaga
    13921 acttcatcga attgctggcg caaaatattg actgttaaag cggtagtcaa tgttttaccg
    13981 tttgttccgg tgacaacgac gacttggtag tcttttgcca aactatctag tatatgtgga
    14041 tcgactttca gcgctagttt acctggtaaa cttgatccgc ctttaaaaaa agtttttaat
    14101 gcccattgcg acgtttttcc aactgcaatc gcaaaatgac tgcgtattcc cattatgtaa
    14161 cctccaaaaa cgatttgctg acgaataaat tcaactaatc ataccatatg ttgaaaaaaa
    14221 ggttaaattg cttttcttat tcttaagaaa aggttacggc tgaaagtgca tgaaatcgga
    14281 caaaatcgca tgatttaaca aaaaaacagc ttgaaacaaa atgattgttt caagctgctg
    14341 tgattttatt attccctctt acctatattc ccattttaac cgataataat tttctcgctt
    14401 ggatattcat agcctagatc tcgtgtttct tttggcacga ataacatcaa tgtatatagc
    14461 attccgatcc gcccaataaa catcagtgta gcaatcgtga ttttgcccac aacagacaag
    14521 tctccggtaa tccctaaaga taaaccagtt gttccaaacg cagaagttac ttcgatgatg
    14581 atcgagatca gcgtttgttc ttctgtagca gataaaaaaa cgatacaaaa gaaacacata
    14641 ccaagagaaa gcataaagac aacgactgat tttcgcacat catcttgatc gattctccgt
    14701 ccaaaaatat tgatgttatc ttcacttttg aggaaggaat atagatacaa accgatgatt
    14761 gctacagtag ttgttcgaat cccgccgcct acagaactag gactgcatcc gataaacatc
    14821 agtaaagaaa aaatgatcag tgttgtgatt tgaaaatcac ccaaatcgtg aatctgcaga
    14881 ccagcattac gagtcgtaat cgaataaaac atcgaattga tccatttgac actcatattg
    14941 gaatcttgga ataaatggtc cttttccaat agataaatca agacagttcc cgaaacaaac
    15001 aagataacaa aagctaacac agctaatttg gtaaacaagg aaaaacgaaa cggaagcttc
    15061 gcattggaac gtttgtataa tagccattcc cggcactcca tcaatacagg aaaaccaatt
    15121 cctccgacaa aaatcaaaaa catgatcagg atcaagaaaa aatagtcatg tgcaaagggc
    15181 ttgatggaat ccccagtgac gtcaaaccca gagttcgtga cagccgatat cgcctgataa
    15241 aaaccgtaaa aaacggcatc acgccaacga tcaaaatagc ctctgtaata aaaataaata
    15301 gaaaagaatg tcccaaaaag taattgaaac cagattaaaa tagcaaaagt gatccgaatc
    15361 agtcggacga taccagacaa acgcggttga ttcatatccg tcatgatcag ctgtcgctgt
    15421 ttcagtgtga tccgtcgttt agaaaaaatg acaaaagcag tagaaatcat catgatcccc
    15481 aaaccaccaa cttgaaataa tatttccagc agtataatac cgttatcatt gaacacagaa
    15541 ttgatatcaa aagtcgatag tccagtcaca ctcactgtac tgattgccat gaacagcatg
    15601 tccacaaaag aaacatggct atctggttcg cgaaaaatcg gcatataaaa tagaacgagt
    15661 gataagaccg tcatcaatat ataataaaaa acgatgattt ggatcgatga gaaatgattg
    15721 gcagcaaact tctgtcctcg ccgctgcagc cttcgaaaga ataaatcaac atgcacagta
    15781 ctatcctcct gacaatagtc gtttctacca gtatacaagg ggaattagga atactcaaag
    15841 aaattatcaa acagataaca atacagcttt cgattaaaat agaaatggct tataataagg
    15901 aaaatcctag aaaaaagaag gaggaggaat aacaaattga ttaatgctca acaaatcgta
    15961 gaaaaaatga aaaatcaaaa aataaattat gatacagttc tgaaaaaaat gatcatgcaa
    16021 tgggagaaag aagaagtaaa gcctaagata ttaatacata catgctgtgc tccttgcagt
    16081 acgcacacat tggaattcat gtctcaatat gcagagatca cgttattttt ttcaaattcg
    16141 aatattcatc ctaaaagtga gtatttgcga agattacatg aacaaaaacg atttgtagac
    16201 caatttaatg agaaaactga ccatcatgtt gcgctaatcg ttgatgaata ccgtccaaat
    16261 gaattcacca aaatggtttt aacaaaccat cttgaaaacg aaaaagaagg tggtgctcgc
    16321 tgcagtgcat gcttcaatat gcgattagat cttgttgcgc aaaaagcgca agaactagga
    16381 tacgattact ttggaagtgc cctgaccatc tcgccaaaga aaaatgcagc tttgatcaat
    16441 cagatcggaa tggatatcca aaagatttac gggacacaat acttgccaag tgatttcaag
    16501 aaaaacaaag gctatgaacg ctctttgcaa atgtgcaaag agtatgatat ttatcgccaa
    16561 tgttattgtg gttgcgtgtt cgctgccaag cagcaaggat gcgatctgaa agaaatcaat
    16621 cgggaagcaa aagcttactt aaaaacatca accgtagttt ttgaggaaaa ttaaagaaat
    16681 ctttcggaaa aacgtaagaa aaaaataaag aatttatggt agaaataagg tagactgtat
    16741 aatttgtccg tagtaaagta aggaaacaag aataaatttc aatgaaggaa gagataagat
    16801 gcgaaaagta gcacctattt tgaagccaat cgctgtatta acaatctcga cgattgtcgc
    16861 gactactaca ggtattgtga atagttccag ctaccgtgca ccaagttcaa gttctagtac
    16921 tgtagaagaa acaacaccaa ctacttcagt ctcgacatct gaaccaacaa caacggaaag
    16981 tttgaatgaa accactacga caagtacatg ggacgataca acaactagtt cgtcgtatga
    17041 tgagacatat acaactgaca gctcaagcga aacaagtaat acggtaccag atggcactcc
    17101 atcggattca accaatccaa gtacaagtcc ggatacaagt gctagctccg aaacgatacc
    17161 aagcagtacg actgtagatg ataacacgca aaatgatgat gcagcacgta cagcacagca
    17221 aaaccaagat tcaggaaata cccaaacgta taaccagcaa gcaggtgatt ctcaatgaca
    17281 tttttactag accttatcaa cggggtgcat aaattccttg gctatttaga catcagtcct
    17341 aaatatctta accgtggata tacgatttta agtgtgattc ccacgcttta tttattgaga
    17401 attgtttatg gactttggca aaaccaaaac tatcttcaat ttttcttata tggacttgca
    17461 tttctagtgc ttgtttattt tactgtttta aatgtatttt attacttttt agataaaaac
    17521 acgaaagccg atgtcactca gctttttgtg aagtatttac ctgatgaagc gttcaacatc
    17581 cagacagaag aatcgataga taatggaaat tcgatcgata aagtgaatac acaagaggtc
    17641 atggtttctt atgacgaaga ttatcaattg aaattagccg agaatatgcg gtatttgatt
    17701 ggaaatggtg agatcaaaac aaatgaactc ggtcgcatgg atggatttct ggtcgataga
    17761 aatacattgt atccttatta ctatgtcaag aaaacaggag agaaagagta tgttctaaat
    17821 attggacgaa gctactcgga catggaaaaa gtagggacca ttcgacttga ctcaacagaa
    17881 gacacattgg aacctgtcgg attgttcatc gtcggcggtg attttgtcaa agacgggtta
    17941 cgctatcatg agccttatcg tctgaaattg attgcaaaaa aggcacagtc gcaggaagaa
    18001 ccagtggttt atagtcgtag ccaaagaaga agacattcat aatgtgatga aacgccgtgt
    18061 atcagtggtt gcgataaaaa cgtttattcg gaataagggg gatgtgacac gtcttttgtc
    18121 acgttccttt tttatataac gagaaagata agacgaacac atcacttatt ctttcctttc
    18181 atttataaac attgtagaat aaagaaagta caacgaagta ttgtgaaaaa acgtaataaa
    18241 taacgaggaa aatacaagta ggaggtaagc tgtgaaagaa tttttgaaaa attgggggat
    18301 tttgatcctt gtattagcgg ctattttatt agctagagtg tacgtgttta caccagttac
    18361 tgtaaacggg cattcgatgg accctacatt aagtgatgga caacgattga tttcatctaa
    18421 gatctcaaac tatgaacgaa tggatattat tactacaaag gaaccggggg atgaagaacg
    18481 gatgattgtt aaacggatca tcggtatgcc tggagataca gtcaagatgg aaaatgacca
    18541 gttgaccatt aatggcaaaa agtatgatga accttatctg gatgagttca aaaaagaatt
    18601 ttcagaagat aagttacaag gagaatatgc ttatagctca ggattccaag cacaagcaga
    18661 aagttcatcc acctttacga gtgactttga atatactgtg ccaaaaggaa agtatcttgt
    18721 tctaggcgat aaccgcttga tttcaaaaga cagccgaatg tttgggttag tcgacaaaga
    18781 tatgatccaa ggaaaagttg ttttccgtta ttggcctctt tcagaaatta aaatcatgtg
    18841 atgaaaaaat aaaaagaagc tgtgacatgt tttttgtcac agcttctttt tccgaaaatt
    18901 ccttcttatt tcttggagtt gctcactttt tttgcaatct cattttaagc tttttggctg
    18961 atggataaga aaagcgaagc gtttttgttc ataaatagct tttccccacg atggaaggta
    19021 tgactagtag cagagacaaa gcccgttttt tccaatcgat tattcaattc ttcttggcta
    19081 aagccattat ggactttcgg atgactaatt tgttcattct tgtcaaagtc tacgatgatc
    19141 aattgaccgc caggagctaa aatttcataa agttttgtaa gaatattctc cgtatcagga
    19201 atgtgtaaaa gtacaagtga tagaaggatg agattagctt ggacagcagg aaattcgaca
    19261 gaagcatcag cgtggatcgt tcggacattt ttcagatctg ctgcttggat cttttcttct
    19321 gccattttca gcatagtttc tgaagcatcg gcaatgatca gttctttgaa tctttccgca
    19381 agcggtaaac taacaagccc agttccgcct ccgtaatcca gcaggacttt ttggtggctg
    19441 tcgcgcggta gataagtagt tagttcttcg cgaataatct tggctaatgc ttgacggtca
    19501 ggggaatcat aatggttagc gatggaatca aaaatatttt ttgtcatcaa atagctcctt
    19561 acgtaaaact ttgaaatttt aacttattat agcacgtgaa aagaaagaaa gcatgctata
    19621 ctcaattcaa aaagggagaa tctagaaaga atatgccgat atcgtttgta aaagatagag
    19681 aggaaaaagg aaagtgtgtc cgagaaatat tactagactt gcctgagtgg tttgggcttc
    19741 ctgaatcaac ggaaaaatat atcgaggaat caagtaaact acctttatgg tgtgaaaaaa
    19801 gaaaagaaga atatttaggc tttatcactc tttcacagac aagtgaagat actgccgaaa
    19861 tctattctat tgtatgggaa taaaaaaaga ctatcatcac caaggattag gaacaaaact
    19921 ttttaaagaa gcagaaggtt acgcagcaaa acattacaaa tatttgcaag tgaagactgt
    19981 agacgagggg cactactcta tatatgatca aactatttgt ttctacgaat cattaggatt
    20041 ttcaaggttg gaagtttttc caaatttatg ggatgaatgg aacccatgtt tagttcttgt
    20101 caaaaaattg gaacaaaagt gagcaataga tttaatacta atctgtgctt gatgaaaatt
    20161 ctcactgtaa aaaatgctga aaatcaagta taataccata ggaacaaact ggaaggtgaa
    20221 aaagagcatg gcacaactat tttttaagta tggcgcaatg aatagcggca agacgatcga
    20281 gatcctaaaa gtcgcacata attatgaaga acaaaataag ccagttgtct taatgacaag
    20341 cggtattgat acaagagatg gtttaggcgt tgtttcaagc cgaatcggac tgaaacgtaa
    20401 tgcgatgccg atatttgaag aaacagacgt atttgacgta attcagaaaa tggaaagcaa
    20461 accctattgt gtgctgatcg atgaagcgca attcttaaaa aagaaacatg tcattgcttt
    20521 tacgagaatt gtggacgaat tggatattcc ggttatggca tttggtttga aaaatgattt
    20581 tcgaaacgag ttatttgaag gatcaaaata tttattgctt tatgcagaca aaatcgaaga
    20641 aatgaaaaca atctgttggt tctgccacaa aaaagcaatc atgaatttac attatattga
    20701 tggaaaccct gtttatgaag gcgatcaagt gcaaattggc ggaaatgaag cttattaccc
    20761 cgtttgtcga aaacattact ttcacccatc aatgattaca gaaggagaat aagcatgtac
    20821 gatcaattac aagcaattga agaccgttat gaagagcttg gagaactttt aagtgaccca
    20881 gaagtgattt ctgatacaaa gcgttttatg cagctttcaa aagaagaagc aaacacgcgc
    20941 gaaacagtcg aagtttatcg tcgttataaa gaagtagtac aaggaatcac cgatacagaa
    21001 gaactgcttg gtgaaaaact ggatgatgaa atggctgaac tagcaaaaga agaattatct
    21061 gaattaaaaa aagaaaaaga agtcttagaa gaaaaaatca agatcttgct tttaccaaaa
    21121 gatccaaatg acgataaaaa tatcatcatg gaaatccgtg gagcagcagg cggagacgaa
    21181 gcagcattat tcgctggcga tcttttcaat atgtaccaaa agtatgctga agcacaaggc
    21241 tggaaaacag aagtgatgga agctaacgtt accggaatcg gtggatataa agaagtgatc
    21301 atgatgatca ccggagacaa cgtatattcg aaattaaaat atgaaagtgg tgcgcaccgc
    21361 gtccagcgtg ttccttcaac tgaatcgcaa gggcgtatcc atacttccac cgcaacagtt
    21421 gtcgtgatgc cagaagctga agaagtagag atcgatattg aagacaaaga tatccgtaca
    21481 gatatctatc atgcttcagg tgctggcgga cagcacgtca ataaaacagc atctgctgta
    21541 cgtttgactc acttaccaac agggatcgtt gttgctatgc aagatgaacg ttctcaaatc
    21601 aagaaccgtg aaaaagcaat gaaaatcttg cgtgctcgtg tgtacgataa gatccagcaa
    21661 gaagcacaaa gcgagtatga tgccaatcga aaatcagcag tcggaactgg ggatcgttca
    21721 gaacggatcc ggacgtataa tttcccgcaa aatcgtgtga ctgatcatcg tatcggatta
    21781 actatccaaa aattagatca gattttagca gggaaattag atgagatcgt tgatgcattg
    21841 atcatgtatg atcaaacatc aaaactggaa gctatgcaaa atgggtaaaa tgacctatcg
    21901 agaagtcctg tcacgggctt cttctttttt agaggatcat gggaaagaag catatagtat
    21961 ccaattttta tttttggaac gaaaacaatg gaaaaaactt gattggctcc ttcatatgaa
    22021 cgaagagatt tcagaagaag aacagcggtt gatcgaaacc gatctgcgac ttctgcttgc
    22081 agatcatcca cctcagtatt tgctaggata tgcagatttt tatgatcatc ggttaaaggt
    22141 aactgaagca acactgatcc ctcgaccaga aacagaggag ttagtcgaat ggtgtttaga
    22201 cgagacgcct ggtgtaccgt tagaagtgat agatattggg acagggactg gagcaattgc
    22261 tattagtttg aaagcagcaa gaaaaaattg gcatatatct gctgtggatc tttcagaaga
    22321 agcattagaa gtagcgaaag aaaatgctca gaaagaagga acgaaaatca gtttttatca
    22381 tggagataca ttaggaccag taatggatca gcaatttgat gtgattattt ctaatccgcc
    22441 gtacatcagt cggaatgaat gggaactgat ggatgaaagt gtccggtcat tcgaacctaa
    22501 aatggccctt tttgctgaga acgatggatt agctatttat gaaaaaatag cagttgaagc
    22561 accaagcgta ctaaaatcaa atggaaagat ttttttagaa atcggatttc gccaaggtga
    22621 agcagtaaaa aaaatatttc agcaagcatt tccagataaa aaagtggcga taaaaaagga
    22681 tctttttggc aatgaacgaa tgatccaagt ttatggttga agttgtggat gaaaaatttt
    22741 ttatttgttt ttttattata aaaaaacact tatttgacaa ggatgtaaac gactttttgg
    22801 tttatcaaca agtttatcca ctattgacaa atagattatc cacaaacagt ggaaaacttg
    22861 aggataaact cagacaaaat aagaatgtat gaaaaagggg ttgtggataa actgtggaaa
    22921 cgaaacgata tgatgaaaca gaattgcaag aggcagccga agcactgaaa gaaggagaac
    22981 ttgttgcttt tcctaccgaa acagtatacg gattgggcgc caacgctctt ttaccaaacg
    23041 ctgtcaagaa agtgttttct gtaaaaggga gaccgcaaga taatccatta atcgtccatg
    23101 tcgcctcttt tgaacaagta aaagagtatg tggacaactt tcatccggca gcagagaaga
    23161 ttgtgaagaa cttctggcct ggaccattaa ctttgatatt taagatcaaa aaagacactt
    23221 tgccctcagt tgtgacggga ggattatcca cagctgcatt tcgtataccg gataacaaaa
    23281 aaacactaga agtaatcgaa ctttccggtg ttccgcttgt cggtccaagt gcaaatacgt
    23341 cgggaaaacc aagtccaacg actgcagatc atgtatatca tgatctacaa ggaaaaatta
    23401 ccgggattat tgatgatgga gctacgcgaa ttggtgtaga atccactgtt cttgatttaa
    23461 gtgatccaac agccatgcct atgattttac gaccaggtgc agtaacgaaa gaacaaatcg
    23521 aagcagtcat cgaatctcct gttgctattg atcagcatct tgtaaaagaa aatgaaacac
    23581 caaaagcccc tgggatgaaa tacaaacact actcacccga tacacgtgtt ctgatggtca
    23641 gagatggcga ttggtcaact gcagttcaat gggcaaaaaa taagaagatc cgtgcaggtg
    23701 tcatagcaag ccccgaaatt gcggatcaag tgcggacaga tactgcagct gtttatatgt
    23761 ataacgacaa ctcagtagaa gcggcggcta aaggattgtt tgcaggtcta agaggattag
    23821 atgaaccaac acttggatta gatttgattt ttgtacaagt gtatccagaa actggattgg
    23881 ggaatgctta catgaatcgc ttgaaaaaag cagcaggaca aaattatttt gaaaaatgat
    23941 caattggtta ttgtcgcatt cttttagtat gatacaatgc tatcaagtaa atcttctatc
    24001 agtgaacgaa aaactaagcg taactaatcg taatagaaga gatacgaata tttggcaatt
    24061 tgtttttgat gggaaaaata aaattgtagg aaaataaaaa ggagctgttg tttgtggtag
    24121 attacaaaac gtttgaccct gatctttggg ctgcgatcgc caaagaagaa gaaagacaag
    24181 aacataattt ggagctgatt gcctctgaaa actttgtctc cgaagcagtg atggctgcac
    24241 aaggaagtat cttgacaaat aaatacgcag aaggttatcc agggcatcgc tattatggtg
    24301 ggtgtgaatt tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa gaattattcg
    24361 gagcgaaatt tgcgaacgtc cagcctcact ccggctctca agccaacacg gctgcttatc
    24421 tcgctttggt agaaccaggg gacacgatct taggaatgga tctttccgca ggcggacatc
    24481 taacacatgg atcaccagtc aacttcagtg gcaaaaccta tcactttgtt gcttacggtg
    24541 ttgatccgac aacagaagtt attgattata atgtggtacg tattttagct agaaaacatc
    24601 aaccaaaatt gatcgtagca ggtgccagtg cttacggacg aacgatcgac tttgcgaaat
    24661 tcagagaaat cgcagatgaa gtaggcgcaa aactgatggt agatatggca catatcgcag
    24721 gattagtagc agcaggactt catcctaatc cagttcctta tgcagatatc acaacgacta
    24781 ccactcataa aacattgcgt ggccctcgag gcggcatgat tttgacaaac gatgaagcat
    24841 tagcaaaaaa aataaatagt gcggtatttc ctggtattca aggcggccct ctagagcatg
    24901 tgattgcggg taaagcagta gcattcaaag aagcattaga tcctgcattt aaagaatata
    24961 gtgaacaaat catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa gcaattggaa
    25021 ccagagtcat ttcaggggcg acagataatc atctgatgtt gatcgatgtt cgcgagttag
    25081 gtataaatgg aaaagaagca gagagcattt tggacagtgt gaatatcact gtaaataaaa
    25141 actcgattcc ttttgaaaca ctaagtccat tcaaaacaag cggtattcga atcggcacgc
    25201 cagcaatcac tactcgcggg ttcaaagaag aagatgctgt aaaagtggct gaactagttg
    25261 tcaaagcatt acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa acaggtgtac
    25321 gcgaattaac tgaaaaattt ccgttgcata aaaaataaaa atttaaattc cattagtaaa
    25381 cagaaaagtt caatgttggg cttttctgtt tattttttgg gaaaacggat tctttgacaa
    25441 tatcggtgat gctctctagt atatgaggtg taattttagt cttcacacta aatctcaggg
    25501 aggaaaagtc atgactgatc gtatctcgtt agaaaaggaa gcagtagcat tcagtgatgc
    25561 aaatgcgcct catccgcgta tctatgaact agcaccggaa gaaggaagag ttttactaga
    25621 agaagtacaa gaatcacctg tgaacaaata ttccgtcgat attgaagact tgatggtgga
    25681 tactggcgaa tggggaaaac tcaatattcg tctcgtacga ccagaaggga atatggataa
    25741 attaccagtt attttttata ttcatggagc aggctgggtt tttggcagtg ctcaaaccca
    25801 tgataaattg atcagagaac ttgcggttcg tacgaattcc gtcgttgtct ttccagaata
    25861 tagtcgatca ccagaagcaa aatatccaac agcaattgaa caaaattatg ctgtgttgca
    25921 acagctgaat aaattagcca agtcaaaaaa tctggatctc gagaatctaa tcgttgcagg
    25981 agattcagta ggtggtaaca tggctacagt catgacgatc ttaacaaaag aacgaaaggg
    26041 cctcccaata tccaagcaat tgctgtatta tccagtcaca aacgctgatt ttgatacaga
    26101 ttcctatcat gaattcggag aaaactattt tttgacaaga gaaggaatga tgtggttttg
    26161 ggaccaatat acgacgaatg aagaagagcg tgctgaaatc acagcttcgc ctttgagagc
    26221 aaatataaat gaactaaatg atcttccttc agcaatgatt ttaacgggag aagcggatgt
    26281 attaagagat gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg ttcctgttac
    26341 acaagtacgc ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta tggatcagac
    26401 aaaggcaacg cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa aagaataagc
    26461 tttgcttgtc cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa aaatggcgag
    26521 tttgactagt caatcacttg attttgtttt acaattaaaa agaaacgaca gaacaaagga
    26581 gagcttaaaa aatgggcaaa tttcaagtga tcgatcatcc attgattcaa cacaaactaa
    26641 ctattattcg ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc gatgaaatcg
    26701 ctatgttgat ggcatatgaa gtatcacggg atatgccatt ggaagacatc gtgatcgaaa
    26761 caccgatcac tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg attattccaa
    26821 tcttgcgcgc aggtattgga atggttgacg gtatcttgga attgattcca gctgctaaag
    26881 tgggacatat cggtctttat cgagatgaag aaacattgga accacatgaa tatttcgtca
    26941 aacttccaga agacattgat gcacgtcagt tgtttgtagt agatccaatg ttggcaacag
    27001 gcggatcagc aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg aacattaaat
    27061 ttgtttgttt ggtagcagca cctgagggag taaaagcttt gcaagaagcg catcctgata
    27121 ttgacatcta tacagcttct ctagatgaaa aattgaatga acatggttat atcgttcctg
    27181 gactagggga tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg aaaaagggtg
    27241 tgacacaact tttgtcacat ccttttttgg tcatttttaa cgtttgatgc agaacagctt
    27301 tttcacagct ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga tggtcaaatt
    27361 caaatctttt ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag
    27421 ttcgcatttt ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa
    27481 atcgttccag tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat
    27541 ttttcaccaa tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc
    27601 ataaaacctg cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa
    27661 ctaattttcc atcggactct ttacaatcag ccaaatagag tatataattt cttttggata
    27721 aggccagctt agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga
    27781 tccctatagc tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata
    27841 catctgtctt tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta
    27901 tttctataaa aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca
    27961 catgaactgc ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca
    28021 atcttgccac cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat
    28081 cataagtact gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa
    28141 tgctgaactt actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta
    28201 tcatgatagg caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg
    28261 ttaaacccca tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg attactacat
    28321 tgataaaaaa atatttggct atatttttga caggagcgac aggtttcgag aacatttttt
    28381 tcgtatcttt tttaccaaaa acaagtgtaa atgctataaa tccagtgata gttaaagaaa
    28441 aaatgctaat aatggatggc gcaaatagca accctaacaa accgagtaca acgacaatag
    28501 tgcccaatat ccctttttta agttctattt tttttcctaa aatgttattc atgataataa
    28561 ctcctactat tcagtttttg aagatagagt cagtatacat gaagagttaa aataggcaat
    28621 gtttttttat ggatatttgt attgctaaaa atttatctta tatattcatt ttgattatac
    28681 tacttttatt tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat taagcgtaca
    28741 attttttaga tgttccgagc gtacttcaat catttcaaag gagttgctac ttaaatcgat
    28801 attttttaaa tcagtatcaa aaaagctagc attttctaaa tcattagtag aaaacattat
    28861 tttagtgagg gaacagtcta caaaatctgc gcttgataag acagagtgct caaaggtact
    28921 ttcttttatg tttgaaccag ataaatttac atagtccatt ttacaattct cgaataacgt
    28981 gttcccaata taactttcca tcaatttagt acctgttaat ttacaattga caaatttcac
    29041 gcgatgcaac atgctttttg tcaatataca gcctgaaaaa tcacagtttt cgaatataca
    29101 gtcggtaatc tccattcttt gaaaatcatt ggaagcaaaa aatgcatact tgaaaataga
    29161 tttgtaaata attggatggc agattatttg gttttcgatg acctcttttt gtgaaaggat
    29221 attatacagt tcaaaatctt cgttttcata gatatgaaaa aaggatcctt tattcaagct
    29281 tgcttcatta actttaggaa aaatcatatt taattacctc cattttcata aagtcatatt
    29341 aacaaaagct aaaccaactt acattagcag aaaatgagat aggttttcgc ctttttcgat
    29401 aacgatagtg tcgctatctc ttgaaacgtt cttagaattg ccctgagaga cacaaattag
    29461 gcgtaattct aataaaaatc ttagaataaa aatttttact gatttaattt agaatgtttt
    29521 taaaataaaa atcgttgcca taacagtgta ttgaaaggtt ttaacaatcc agtaatgatt
    29581 taaaaaaatc tagatgtagc gtatactttg tttatagaat gtagtgggaa gaaatatatt
    29641 tttgggagga aatcgtgatg tctaaaagtg aagaaataga aaacatggta ctaaaattat
    29701 atgatctcgt tttaaatcca gagatcaaag aaaaggaacg tatacttttg atagacgcaa
    29761 agacaggctt agaaaaaggt cagtattatc ctaaagtcat aaataatttg gaacgtagtt
    29821 tacgtccgtt agcaatcagg ggagaattgt ctaaaccagt atcaccgttt tatatggaga
    29881 tatcaacaat aggaaaattt gaaaaagaat taggtcgagg aatggcttcg gcaccgatta
    29941 cttttgggca tttataaaaa cgagttattc atctcaatga attaaaaagt atagtcaatc
    30001 tcataatgca ggctaggttc atttatttga acagagtctg tattttttta ttctaaataa
    30061 agaaacagat gtttctttat attgtatttt atcctttaat taacacaaaa acagctagta
    30121 taaactaata atgttaatta aaagtgtatt ttatgaaact atcgttattt attaatgttg
    30181 catttttgta agttaaaaat tggaaatata gattgaaaaa atgaaagcaa taagtaaaat
    30241 aaaatgtata tatgcgaaaa aataatttac attaggaaaa accttgtaaa aacagttttt
    30301 tggcatattt attttttata aatatttgta aaataactat caatttttta gttaaaagat
    30361 tggaaatgag tgggggagga attgaagaaa ggatttttga gagatgatat agtaggtttt
    30421 atttttagtt aaaatagggg agatagaaaa aatgaaaaag tgtagaaata ttattttatc
    30481 aatgttattt attattacta atataacatc actgattcct gttcatgtct atgcggatgc
    30541 aggcagagat atcagcagta atgtcacttc gttgaccgtg gacccaacga atattacaga
    30601 tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa aaacaaaata tccaaccagg
    30661 tgattatctt tggatcaatt ggccaagtga aggaaatatt cgcggtgaag gattccaaaa
    30721 agaaatccca ttaatgattg aaaataaaaa tgttggaact ttaacggtca gaaaagattc
    30781 tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac tcagtagaag gttgggggca
    30841 atttgagata caggccagaa acgtaaccga taccggtgaa gaaaatacgg gaccattcac
    30901 cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca aagccagctt ctggttcttc
    30961 ttctagcgtt ttttattata aaacaggaga catgctacct gaagatacta agcacattcg
    31021 atggtttttg aatattaaca ataacggcac ttatgttgaa caaccagtta aaatcagtga
    31081 tgaaatccaa agtggacaaa gattagatcc aagtacgttt gaaattaatc aaatacactt
    31141 aggtgaacag aaggtttata gaggagaaga gggaatccaa caatttttac aagatttccc
    31201 cagcgctaca tttaattttt cagtaactga taattacatt gaaataacta taccaaaaaa
    31261 ctttgtcaat cttaggaaaa taatggtgag ttataagacg atcatagaaa acccagaaca
    31321 aataaatttt gagaatcatt cggaagcttg gtttaaagaa tttaataaac ctgctgttga
    31381 tggagaaagt ttcaatcata cagttaaaaa tatttcagct tctggtggag tcaacggtac
    31441 agttagaggc gaattgaaga ttttcaaata tatcaatgac acagagattg gtatcccaaa
    31501 tgtgactttc gaattaagac gagcagatga acagcctatt caaggacagt ctagtatcct
    31561 gttgacttcg aatgaacagg gagaagctag tatcaaaggc ctgcaagtag gagactatgt
    31621 tgtaaaagaa aaagaagcac ccaattggat tgattttgat ccactaagtt caaatgaact
    31681 caaattctcc ataaacgaaa atgatacaga gggagtcagt ttacctattt ataacaagaa
    31741 aaaggttacg aacatcacag caactaaaat ttggaacggt ggaaccactc caagaccgag
    31801 tatttatttt aaattattca gagcaagtac aaacaattgg gaaccagttc ctgatgcaga
    31861 aacaaaacga ttagacaatg gaataacttc agtgacttgg gaagatatcc aacagtatga
    31921 tgatagtgga aacgaatata cgtttaaagt gcaagaagta gatcaaaacg gaaatgatta
    31981 tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc gttacaaatg agaataaaga
    32041 agtcatttca gtctcaggac aaaaaacatg ggaagacaat gagaaccaag acggcaaacg
    32101 accgacgacg atcacagtaa atctattagc cgacggtcaa ccgatccaac acaaagaagt
    32161 aagtgaaaaa gatgattggc gctatcgttt cacaaatctg ccaaaataca gagacgggca
    32221 agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa tacagtacga cgattgacgg
    32281 gtataacatc aaaaacagtt atacaccgga aaaaacaaat atatctatac caggtagcca
    32341 tataacaccg tggaaaaatt ttccagagaa gacagagaga gagaaaatta gaaatctatt
    32401 ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc agcgaacaaa gaagcaatta
    32461 tattagtaag agagctgtac ctaaacagat aacaaataaa tcaaaaagca ttcttcctaa
    32521 aacaagtagt aaaaaatcta gttttgctgt aatcattggt ttattgatag taactatctg
    32581 tgctgggata ttcttacaaa aatataaaaa agtttagtta gaatataata ttttttgtta
    32641 aatatgactg aaatcaaaag aataaaattc cgatttataa tcgaaaaaag aagcattttt
    32701 atatatgctt cttttttcgt agaatcaata aatatggatt tggctaacta tggaagaata
    32761 ctcgtattag ttttcagcgt tcatactagt tgttacattg tttgattggc caaaagaatt
    32821 tctgatatat tttgtgctaa ataaagaaaa agacgcttga attgggaaat aagaaaacta
    32881 ctctatcatt acaaattttc ataaactggc agatgttcta agtaaacaag tatcttttca
    32941 aagtcggaaa aatccataat caacgtagtg gtattgatat ttggatgaaa gcctacggta
    33001 tcttgacgat caatagcagc atcgatcacg acttggattt ttttctcttt gtcgtgcatc
    33061 aatgctaatg gagtcactgt acctgctgga acgcctagga ggtcaggaag ctgttcttca
    33121 gaaacaaaag ataagcgctt ttcttttaat tgttcagcta gttgttttaa atcggcgtgc
    33181 ttttcatcag gcaagatgac aagatagatt tgttttcctt ttcttgattt caataataga
    33241 ttttttactt gcggaccagg tagggaaaaa gggacatttt tgactgacgt aatcgcagga
    33301 tgatcgactt tttgaaaaga gatacccagt ttttttagta gttcataagc ttcttgttcg
    33361 gttgcacgac tcatcgcgca tcaccctcct ttttttatta gtgtatcata aaaaagagtt
    33421 gacaaatgaa aaataatcat gtaaattaca cgtaaagtta aaacaaatga catcgaaaag
    33481 aagagtagcc gttaggtgtt tttagagagt ctctggttgg tggaaagagg cagcagttaa
    33541 ccgtgaaaca catcttggag tcgattttct gaagcgtata gtaggaaaat ccggggagcc
    33601 cgttacagct gcggtcgttg ctagatgacc agataaggct ggcttttccg tgaggagtcg
    33661 gtaaaaaaag gtggaaccac gtgattaacg tcctttgatc ttcggatcaa aggacttttt
    33721 gttttcatct gaacggcaac gactgtgtga ggttttttct gcaaggaaaa aataaacaga
    33781 ggtggaacca cgtataatac gtcctcttgg catttgccaa gaggattttt ttattttaaa
    33841 ggaggaaaag aagatggagt atttgattga agttatgccg caattattag taggagcact
    33901 cacaacattg aaattatttt tatttacctt gattggctca ttaccattag ggattgtttt
    33961 agcattagga ttgtctttgc attttaagcc gctacgctac ttgcttcaaa catatgtatg
    34021 ggttatgcgg ggaacaccgc tgttgttgca actgattttt attttttatg ggttgccgac
    34081 gattgggatc gtttttgatc gtttcgaagc agcactcatt gcctttatct taaactatgc
    34141 agcttatttt gcagagatct ttcgtggagg gatacaatcg attcctcaag gtcaatatga
    34201 agcagcgcaa gttttgcaat taagtccctt tcaaacggtg acgaaaatcg tcatcccaca
    34261 agtgacaaag attgttttac cttctgtagg gaatgaagtt atcaacttgg tcaaagattc
    34321 ttccttgatc tatatattag gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga
    34381 acgcgacgcg actttactgc ctttgctagg cgtggcaatc atctatctta cattgactgg
    34441 tattctaaca atcgtaatga aacaagttga aggaaaatta aattattacc gctaactgat
    34501 aggaggaaga aatatgattg aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat
    34561 aatcaaagat ttgaatctaa tcattccaga tgggaaaaca ttagctatcg taggtccttc
    34621 cggtggcggt aaaaccacgc ttttacgtat cttggctggc ttagaaagtt ctgacagcgg
    34681 agaattttat ttagacggtg taccgttcaa cccattcgag cttaaaatga aagaacaagt
    34741 tgtaggagtc gtattccaag acttccagct atttccacat ctgaatattt ttgaaaacat
    34801 taccttagca ccaaaaatgg tattgaaaca gacaaaagaa gaatacacca aaaaagcaga
    34861 agaattagcg gcttcattag ggattagtga tttattgaat atctatcctt atcagctttc
    34921 tggcggacaa aaacagcggg tagcgattgc cagagcattg gctatgaatc cacgggtatt
    34981 ggcttatgat gaaccaacaa gtgctttaga tcctgaatta cgccagcaag tcgagcgttt
    35041 gatcctatca ttgaaagaag atggggtaac ccaaattatt gtcacccatg atttagtatt
    35101 tgcagaaaat gtcggtgatc aattactaaa agtttcccca attcattaag aaaggagaag
    35161 atagagatga aaaaaaccag ttttctattc gctttgatcg caggtcttct tctttttgca
    35221 ggctgttcga acaaaaaaac ttcagcagat aattgggcga ctatcaacga agagaaaaaa
    35281 atcgttatag gtttagacga tacatttgtt cctatgggat ttagagataa agacgggaag
    35341 ctcacaggat ttgatattga tttagccaaa gcagtctttg aacaatatgg gattacggta
    35401 gactttcagc ccattgactg gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat
    35461 ttgatatgga acggctattc caaaacgccg gcaagagaaa agaaagtgca atttacaaaa
    35521 ccttatatgg aaaacgatca agtgctgatc acacctaaag aatcaggaat cacaaacttt
    35581 ggacaaatga ataataagag attaggtgcg caaaatgggt catctggtta tgatgtattt
    35641 acgaaacaac cagaagtatt aaaagagata gtcaaaaacc aagatgctgt tttgtatgat
    35701 agttttaatg aagcattgat cgatctgaaa agcagacggg tcgatggtct gctgatggat
    35761 aaagtatacg cagactatta tttgaaacaa agaaacgaac tagaagactt caacataaca
    35821 aaaggttctt atgagagtga ggattttgca gttggtgcac gcaagtcaga tagtacatta
    35881 gtgaaaaaaa tcaatgaagc cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata
    35941 tccgataaat ggtttggcga agatgttacg ccgagaaatt aacaaatagg ggactgtgac
    36001 aaaagacgtg tcacagtccc tttttccgaa taaacagtgt tctcctgcca actccttgtg
    36061 actcggagct gaatggcagg ctcacaacct cttggtaaac ggtgttcaaa agccagtttt
    36121 tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa atttttctta
    36181 ctactcgaaa ctaagcgact ttttcacaac ctcctttaca taattttatt ttgttgtgga
    36241 aggggtttcg tgttattcta aaagaggaac gattgaaggt tataggaaag gaagagatga
    36301 atggaacaca aagaacttac ctcatttcca aaagattttc tgtggggctc tgcttctgca
    36361 gcgtatcaag tagaaggagc ttggcaagaa gatgggaaag gagaatcagt ctgggatcga
    36421 tttgtacgga tacctggaaa gacatttaaa ggaacgaacg gtgatttggc agtagaccac
    36481 tatcatcgtt ataaagaaga tattgcttta atgaaagaac aaggtctgaa agcttaccgc
    36541 ttctctgtag cttggacacg gatttttcct aatggacgag gagaagtcaa tcaagctggg
    36601 ctcgcttttt atgagagatt gatcgacgaa ttgattgaaa acgatattga acctatgttg
    36661 actttatatc attgggatct tccacaagca ttgcaagatg aatataatgg ttgggaatca
    36721 cgtcagatca ttgaagattt cactcattat gcagaaacat tatttgaggc attccgcggg
    36781 aaagtccatt actggattag tttgaatgaa caaaatatct tcacttcatt aggttatttg
    36841 ttagcagcac atccgccagg tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt
    36901 gctaatttag caaatgcatc tgtcatcaat aaatttcacg agttagaaat gcctggaaaa
    36961 atcggaccaa gctttgcgta tacgccaaat tatccaattg acagtaatcc tgaaaatgtt
    37021 ctggctgctg aaaatgcaga agacctgatg gccaattatt ggatggatgt ctatatgtgg
    37081 ggaaaatatc cgattgctgc catgaggttc ttagaagaaa aaggatgggc accaacgatt
    37141 gaagctggcg atgcagaatt gctagaatct gctaaaccag attttcttgg gatcaattac
    37201 tatcaaacgg cgacgaatgc attcaatcca ttaaacggag ttggtgctgg aaaaatgaat
    37261 acgacaggaa aaaaaggttc atcagaagaa actggagttc ctggaatgta taaaaaagtt
    37321 gagaatccat ttgtcgaacg aacaaattgg gactgggaga ttgacccaga aggattaagg
    37381 atcggactgc gtcgtatcac aagccgttac cgcatgccgg tactgattac ggaaaatggc
    37441 ttaggagagt atgacaagtt gacagaagat aaacaaatcc atgacgacta tcgaatcaat
    37501 tacttacaaa gccacgtcaa agccatcaaa gaagcaattt cggatggagc agaagtccta
    37561 ggctattgca cctggtctta tacagatttg cttagctggc tgaacggtta tcaaaagcga
    37621 tatggatttg tatatgttga tcaagacgaa acacaagaag gatctttgga acgatataag
    37681 aaagacagtt attactggta ccaaaaactg attgaagaaa atggagagaa cctttagttc
    37741 acatacttaa aacaagaagg tacggctgga tatggccgtg ccttttcttg ataaaatcat
    37801 ttagaaatta agaaaaactg taaaataagc tgaaaaatga aagaaagcgt cagttctttc
    37861 aaacaaaact ttagttccct tacttttttt tgtataatga aacttgatga aatgagggat
    37921 aataaatggc acaaaagaaa cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa
    37981 agcaaaaaca gcagcaggaa caaatgctga cgattggtgt tgggtttgcc ttgattttat
    38041 ttgctgtctt cggcttttta aaattaggtt ttttaggaat attgattgca aacggctttc
    38101 ggatcattgc aggaaatact taccaaatcc tctgtctttt attagccgta ttaggatttt
    38161 ggattgtcat taagaataca gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca
    38221 tactctttta tttcggtatc ttattattgt tacatgccca tttatttggc aaactccata
    38281 ctggggaacc aaatatcatg gggacgacat gggatttgtt agccagtgat ataaaacaaa
    38341 gtcaagtaga caataacgtt ggtggtggga tgattggagc aatcctttat cattttactt
    38401 acttcttgat tgcacagcct ggaagttatt tagcagcagt gctattactg gcaggaggag
    38461 cattcctgtt cagtaatctg gaaggctatc agctgttaaa tggtatccaa tcaattggag
    38521 aacgagtaca ggaattattg gaaggtgatc ctgccaagca agctcgaaag caagcggcaa
    38581 aggaagagag aatgaaacag cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag
    38641 aagctgcaga aaaagaagct gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg
    38701 aaaatactgc tgatgaatgg caggaaccag aaaatacaga gccggagcag ctaagttttg
    38761 ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag
    38821 atacaccaaa acagaccaat acagcagaag gtttttcaga tgagttgcca gaggatgacg
    38881 gtacctcatt agaatttgag attgaagcag aacaggaaaa tcaagactat gaattaccga
    38941 cagttgattt gcttgattct ataccaactg tggatcaaag tgatgagtat aaaaaaattg
    39001 aaaaaaatat cggcgtatta gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg
    39061 tcaaagccag cttaggacca tccgtcacaa aattcgaagt ccagccggct gtaggggtaa
    39121 aagtaagtaa aattgtgaac ttaacagatg atatcgcgct tgctttagcg gcgaaagatg
    39181 tccggatgga agcaccgatt ccaggtaaat cactgattgg tattgaagta ccaaatggaa
    39241 agatcagtat ggtttccttt agagaaatca tcgaagcaca accaaaccac ccagataaat
    39301 tactagaagt acctttagga cgtgatgttt ctggacgggt acaaacggct gatctttcga
    39361 agatgcctca cttactcgtg gctggttcga ctggtagtgg gaagtcagta gcaattaatg
    39421 ggattatcac aagtatatta atgcgtgcta agccccatga agtaaaactg atgatgatcg
    39481 atccgaaaat ggttgaattg aatatgtaca acggcatacc tcatctgttg acgccggtcg
    39541 tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa atggaagaac
    39601 gatatgaaaa attcgcggca accggagtaa gaaatatttc tggttacaat gaatttgttc
    39661 aacagaaaaa cttggaaaac ggaacgaaac atccaacctt accattcatc gtagtgatcg
    39721 tagatgaatt agctgatttg atgatggtag ccagcaatga agttgaagat gcgattattc
    39781 gtttagcgca aatggctcgg gcagcgggga ttcatatgat cctagctact caacgtccaa
    39841 gtgtggatgt tatcacagga atcatcaaag caaatgtacc ttccagaatg gcatttgcgg
    39901 tatcaagcgg tacagattct cggacgatca ttgattctaa tggggcagaa aagctcttag
    39961 ggcgaggaga tatgctattc ttgccaatgg gtgagaataa accgatccgt gtgcaaggag
    40021 cattcatttc tgaccacgaa gtagaacggg tcgttcaatt tgttacagat cagcaagaag
    40081 ctcattatga agagaaaatg atgccgacag atgaagttga aacgacaggt gctccagaac
    40141 agccacaaga tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa
    40201 gtatttccct tctccaaaga agatttcgga tcggttacaa ccgagcagca cgtttagtag
    40261 atgaactgga agctcatgga gttgttggcc cgtcagaagg aagcaagcct cggaaagtat
    40321 tcattgagca ggaagaaaca tcagaaacgt tgccggacga taatgacatg taaaacttgt
    40381 gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca aggcatgatg ataccaaaag
    40441 aaaaaatggt ttttttccat cttttctaat acggtatgat gtcttgtctt tttagttttt
    40501 gtgagattaa agttctattc gatttatttg tgaagtattg cattaaggaa atgaatcatg
    40561 tatagtatgt agcgaaactt tgagaaaaag agggaaaata ctttggaaaa acagtccatt
    40621 tatggcttga cgaatgaaga attaatcaat tggtttatag aaaacggcga gaaaaaattc
    40681 cgagctgccc aagtttggga atggttgtat caaaaacgtg taagtaattt cacagaaatg
    40741 acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc attttatcat caatcctcta
    40801 aaacagatgg tcgtccaaga agcttcggat ggtacagtaa aatatttgtt tgaactccct
    40861 gataaaaaca tgattgagac tgtactgatg agacaagaat atggcttgtc agtttgtgtg
    40921 acgacacaag taggatgtaa tatcggctgt acgttttgtg caagcgggct gctcaaaaag
    40981 aaccgtgatc tgactgctgg agaaattgtt gcacagatca tgatggtcca acattatttt
    41041 gatgagcgca atcttggcga acgcgtttca catgtcgttg taatggggat cggtgagcca
    41101 tttgataatt acgacaatgt aatggacttt ttgcatatca tcaatgatgc caaaggattg
    41161 gcaattggtg ctcggcacat tacagtttcc actagtggat tagcacacaa aatcaaggaa
    41221 ttcgctaata acggcttgca agtcaatctg gctatttcct tgcacgcgcc gaataatgag
    41281 gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt gatggaagct
    41341 gtggatgagt atttagagaa aacaaaccgt cgaatcacgt ttgaatatat catgttgaat
    41401 caagtaaatg atagaccaga gcatgcgcaa caattagcag atctattaaa agataagaaa
    41461 aaattagcat atgtcaactt gattccatat aatccagtca gtgaacatga tcaatattct
    41521 cgcagtccaa aagctgatgt attgagattt tatgatgtat tgaagaaaaa cggcgtaaac
    41581 tgtgtgatac gtaaagaaca tggtacagat attgatgcag cttgcggaca attgcgaagc
    41641 aagcaaatga aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt caattaccat
    41701 aaaaggagca gttttcttaa ttaaggaact gttcaactta aataagagaa aagattctat
    41761 agcgaaagct ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct
    41821 caatttgatg acagcctgct tgatgggggt tttagcaatt atttaactga ttttcttata
    41881 tccttgctgg ctttcttgtg tagcatgata agtttctacg tcaagaatgg cctgtttaaa
    41941 tttttctcgg gtgatttcat aaggcacatg tttaatatca tttgttgcca tggctttatc
    42001 taagattgct tctaaatcgg tcgtgacatt gagattcatg tcggttaacg atactggaag
    42061 ttggttcttt ttcataaaca catagatttt atcaaacaaa tcgaactgct gatccatcat
    42121 caataaggtc agtacaccat aactgacaga tgctccgtga aggtatccct ccattttttc
    42181 tagaacagtc gtaccgtaat agaatgagtg tgccatagca gaattatagt tattgttaac
    42241 taaaacagaa accaatccgg tagtaacaat gatatccgaa acgacttgtt caattgcata
    42301 tgagggacga tcgtcggcac tatctttcaa tgcttgcgta ccataagtca agagacgatc
    42361 aatgcacatt tttccgattt ggattcctaa tctatccgtg tagcctagat catcgccacg
    42421 agcagaaaaa gtagattcga cttctttgga taaagcatcg ccaatacctg cccataagta
    42481 tttatcaggt gcttcggcaa taattgctgt atcaatgaag gtgtgtacag gtggttttga
    42541 aggaaagaag agtccgtcca ttacgccttc ttcgttatag atgacacata ccgcagtgac
    42601 gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta gtttttcacc
    42661 aatgactttt acagtatcgc aagcacggcc accaccgact gcaaacagga cgtctgcttt
    42721 ttggacactt tcatgctgga tcaactgttc aatatttcca taagttgaat tacccccgta
    42781 ccagagactg gcgacaacct ctatttcctg tgtcaaagct acttttattt tgttttctgc
    42841 ctttgaaagt gcagtcttcc ctccaatcag cactactttt gtcccatatt ctttgacgat
    42901 tccatttgct ttttcatacg catctgttcc aatggtataa tttggtaaga aaacactttg
    42961 actcatattc atttccttct ttcttcgttg aaacgggtta aaattttaaa gagtgatcga
    43021 tctgactgcg gctgtagatg gagtagaggt gaacctaaga caggataagt gatgaataag
    43081 gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca
    43141 ttgaaaacga caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt
    43201 ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt
    43261 gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca
    43321 gagttttttc ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct
    43381 gtataatttt tttattttat aagtaagata aataatgaaa gaatagagat actgattggt
    43441 agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa
    43501 gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa
    43561 gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga cagctcggca
    43621 gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat
    43681 gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat
    43741 ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac
    43801 ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta
    43861 gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct
    43921 tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc
    43981 gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg
    44041 attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc
    44101 aagaaataag aaggaattta cgaaaattac ttcttgaatt tttgtgaaat tccaacttat
    44161 ttccgaagaa gctacttttg gaacaccgtg tac
//
LOCUS       DACEOM010000014        43597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEOM010000014 DACEOM010000000
VERSION     DACEOM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.015"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(237..2618)
                     /locus_tag="IW781_000758"
     CDS             complement(237..2618)
                     /locus_tag="IW781_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1181775.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2608..3255)
                     /gene="recU"
                     /locus_tag="IW781_000759"
     CDS             complement(2608..3255)
                     /gene="recU"
                     /locus_tag="IW781_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1181776.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3319..3858
                     /locus_tag="IW781_000760"
     CDS             3319..3858
                     /locus_tag="IW781_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1181777.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3948..4367
                     /gene="gpsB"
                     /locus_tag="IW781_000761"
     CDS             3948..4367
                     /gene="gpsB"
                     /locus_tag="IW781_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1181778.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4389..4756
                     /gene="rnpB"
                     /locus_tag="IW781_000762"
     ncRNA           4389..4756
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW781_000762"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4965..6131
                     /locus_tag="IW781_000763"
     CDS             4965..6131
                     /locus_tag="IW781_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1181779.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6177..7673
                     /locus_tag="IW781_000764"
     CDS             6177..7673
                     /locus_tag="IW781_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1181780.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7754..8437
                     /locus_tag="IW781_000765"
     CDS             7754..8437
                     /locus_tag="IW781_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1181781.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9082..10272
                     /locus_tag="IW781_000766"
     CDS             9082..10272
                     /locus_tag="IW781_000766"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1181782.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10374..11327
                     /locus_tag="IW781_000767"
     CDS             10374..11327
                     /locus_tag="IW781_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1181783.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11396..12745
                     /locus_tag="IW781_000768"
     CDS             11396..12745
                     /locus_tag="IW781_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1181784.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12928..13251)
                     /locus_tag="IW781_000769"
     CDS             complement(12928..13251)
                     /locus_tag="IW781_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181785.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13380..14543
                     /locus_tag="IW781_000770"
     CDS             13380..14543
                     /locus_tag="IW781_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181786.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14613..15962
                     /locus_tag="IW781_000771"
     CDS             14613..15962
                     /locus_tag="IW781_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1181787.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16007..16438)
                     /locus_tag="IW781_000772"
     CDS             complement(16007..16438)
                     /locus_tag="IW781_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1181788.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16826..19468
                     /gene="alaS"
                     /locus_tag="IW781_000773"
     CDS             16826..19468
                     /gene="alaS"
                     /locus_tag="IW781_000773"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1181789.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19668..20264
                     /locus_tag="IW781_000774"
     CDS             19668..20264
                     /locus_tag="IW781_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1181790.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20386..21534
                     /locus_tag="IW781_000775"
     CDS             20386..21534
                     /locus_tag="IW781_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1181791.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21572..21985)
                     /locus_tag="IW781_000776"
     CDS             complement(21572..21985)
                     /locus_tag="IW781_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1181792.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22064..22495
                     /locus_tag="IW781_000777"
     CDS             22064..22495
                     /locus_tag="IW781_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1181793.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22594..22827)
                     /locus_tag="IW781_000778"
     CDS             complement(22594..22827)
                     /locus_tag="IW781_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1181794.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23010..24680
                     /locus_tag="IW781_000779"
     CDS             23010..24680
                     /locus_tag="IW781_000779"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1181795.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24827..25213
                     /locus_tag="IW781_000780"
     CDS             24827..25213
                     /locus_tag="IW781_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181796.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25200..25898
                     /locus_tag="IW781_000781"
     CDS             25200..25898
                     /locus_tag="IW781_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1181797.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25903..26367
                     /locus_tag="IW781_000782"
     CDS             25903..26367
                     /locus_tag="IW781_000782"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1181798.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26364..27275
                     /locus_tag="IW781_000783"
     CDS             26364..27275
                     /locus_tag="IW781_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1181799.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27510..28049
                     /gene="pyrR"
                     /locus_tag="IW781_000784"
     CDS             27510..28049
                     /gene="pyrR"
                     /locus_tag="IW781_000784"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1181800.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28063..29346
                     /locus_tag="IW781_000785"
     CDS             28063..29346
                     /locus_tag="IW781_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1181801.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29359..30285
                     /locus_tag="IW781_000786"
     CDS             29359..30285
                     /locus_tag="IW781_000786"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1181802.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30304..31584
                     /locus_tag="IW781_000787"
     CDS             30304..31584
                     /locus_tag="IW781_000787"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1181803.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31589..32665
                     /gene="carA"
                     /locus_tag="IW781_000788"
     CDS             31589..32665
                     /gene="carA"
                     /locus_tag="IW781_000788"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1181804.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32670..35855
                     /gene="carB"
                     /locus_tag="IW781_000789"
     CDS             32670..35855
                     /gene="carB"
                     /locus_tag="IW781_000789"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1181805.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35909..36685
                     /locus_tag="IW781_000790"
     CDS             35909..36685
                     /locus_tag="IW781_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1181806.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36682..37614
                     /locus_tag="IW781_000791"
     CDS             36682..37614
                     /locus_tag="IW781_000791"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1181807.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37611..38327
                     /gene="pyrF"
                     /locus_tag="IW781_000792"
     CDS             37611..38327
                     /gene="pyrF"
                     /locus_tag="IW781_000792"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1181808.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38334..38963
                     /locus_tag="IW781_000793"
     CDS             38334..38963
                     /locus_tag="IW781_000793"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1181809.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38951..39265
                     /locus_tag="IW781_000794"
     CDS             38951..39265
                     /locus_tag="IW781_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181810.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39303..39944
                     /locus_tag="IW781_000795"
     CDS             39303..39944
                     /locus_tag="IW781_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1181811.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39975..40679
                     /locus_tag="IW781_000796"
     CDS             39975..40679
                     /locus_tag="IW781_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1181812.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40676..41578
                     /locus_tag="IW781_000797"
     CDS             40676..41578
                     /locus_tag="IW781_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1181813.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41736..43442)
                     /locus_tag="IW781_000798"
     CDS             complement(41736..43442)
                     /locus_tag="IW781_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1181814.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13960 a         7688 c         9477 g        12472 t
ORIGIN      
        1 gactagtgtg tatttagccg atttaattat aaacaagtaa atggataaag tatatgtaat
       61 gataaaagaa aaatggattt tctaattttt aagctaaatg ttagatcaca catgtatatg
      121 ctggatagtc ttataaaaaa tcatgtaata cagcttggta catgattttt atttgtagct
      181 gttttttaca tagacacaaa aaagaaacca cgcttgtaga cgtggtttct tttttactat
      241 ctgtttccag aagctgaggt gctgctggat ggtgtatggt catttgcagc atccgctccg
      301 ctagttgctg gctcactgga agaagcagga ggagcagatg aagacggtgt cgagctttct
      361 tcggatgaag aagaaggaac cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg
      421 ctttcttttg aggactcagc agttgattcg ctttgactag tagagctaga gctttgagtc
      481 gttgtctctg ttgttgagct tccaggagtc tggacatagc tggaagatgg aatagttgtt
      541 gatggtgtaa ttgcattcga tctagctgta tattgatctt tgtagtacag ttcaccgccc
      601 acacgaatta atccgcttgg catctcccag tctgtatttg tcacgttagc tgaaacatac
      661 tgcataagct cgcgataaac atcagaggct acgtgtgacg attctgaagt gacaggtgtc
      721 atttttttat tgtaacctgt ccacacggaa atcgaataat ttggcgtata gcccgcaaat
      781 aggatatctg gatacacccc agaagaaata ccaagttttg catactcgtc atctgtgtaa
      841 ttggatgttc cggtcttacc ggcttgatat aacccagcga tttgcgcatt cgtaccagtt
      901 ccttcagtga tcgtatcttt taaaatgtct gtgatcatat aagctgtttc tggagacatg
      961 gctgtttttc catctggctc ataagtttct tctgttccgt cttggaagac gattttatta
     1021 acatattgag gtttgtaata agtccctcca ttggcgaaag ccgcataggc tgctgccatt
     1081 tttaaggaag aagctccgta ttttgtccca tcttgttctt ccgtattact agagatcgca
     1141 ttcgattgat ggatcgtact gtattcgatc ccaagatttt ttaagaaaga agccacttta
     1201 tctgaaccga cttcattgaa taatttgact gctggaacat tacgggataa gtaaagtgct
     1261 tggcgtaaag taatggtgcc catatattga ttatcccagt ttcctacagg agtcgaagtc
     1321 ccttcatagt tatacggtgc gtcagtaata gtttttccag tagaatattt caaatattca
     1381 aaagctggtc cgtaatctgt gacaggtttc atcgtagaac caaaatcacg ggaagtattc
     1441 actgctaagt tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc aatctgggct
     1501 tttactttcc cggtattggt gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg
     1561 taagagacat actgatccgt gttgacgata tcatatagtt tcttttgggc gtccagatcc
     1621 aaattcgtat aaatctctaa accgtctgtg tagacattct tatctgtttt ttcttgtact
     1681 tcattgatga cttctttgac ataattatcg acgatcttag tattgtcatc ggattgagtt
     1741 agttcttgca gaccatccgt taccggtaca ttaacagctt gatcatattc tgtttgagag
     1801 attttttcgt tttgaagcat cgtatacaaa acagtatctc gtcttttctt cgcctgatct
     1861 ggataaacat aaggatcata ggcggaaggg gcttgaggca tcccagcaag caatgctgtt
     1921 tgcggtaaag aaagttctga taatttctta ccaaagtaca tctcagaagc tgtctccatc
     1981 ccgtacaagc cgtttgacat atacacttta tttacgtagt aggtcaagat ctcttgtttc
     2041 gatttttttt gttccaatct tacagccatc cacgcttctt gggccttgcg tttcagagtc
     2101 tgatcctcgg cactggtgga gaagaaggaa agtttgatca attgctgtgt caaagtactt
     2161 ccgccttgca gtccgccaga ggtaaagtta gagagggcag aaccgatgat ccgaataggg
     2221 tcaaccccga tgtgtttata aaaacggcga tcttctaccg acacgatcgc atcttctaat
     2281 gttttcggta attcattcgc agaaattttt tcacgctttt ctgcgcctaa atcttcgaat
     2341 agttcgccat cttgtgtata tagtttggaa gatacggtag cgtctaattt tttatccgtc
     2401 aattcgggcg catcttttgc gtaataccag aaaagtccta cacctgctaa aaagaggata
     2461 cagaaaaatg agagtattcc taaaaggatc ttgataaata atcctttttt atgtgttcca
     2521 gaggactttc cagaaccttt gccagaattc ttcttcacac tctttttagg agtaggctgt
     2581 ttctgtcgtc tagagcttct agtttgttca tttgccattc ttgctttctc cttcaataga
     2641 taatgttgat aaatagaagt caaccgcgtc caaataagga atccgcggag aaataccagt
     2701 tttgatctct attcctagtt cttcgatcaa agataaaggt agtgatttgc gtcctttttc
     2761 cttttgggcg tcccagtaag caatcaaatg aacactattc aaataaaaac agcgcttcaa
     2821 agacgaaaac cataacaaga caaagcagac cccattttgt tcgaggcatg ctttcatatg
     2881 ttcgatttga tgggcatgga agtttttaaa gggaaaagat gttttgttct tggtttcttt
     2941 tgcttcaaag tccaagtaaa agcctcgata gacgccatta tagtctgtag ttgaggcttg
     3001 ggtaaagtag gcttccttga tcacagcagc acttctctta ggatagtcta ctttcacgat
     3061 ttggataggg gtaggctttt tatggataac cgccaacccg cgggaaagat aatatttatt
     3121 actttcattg attgcttctt caaagcgcat cccccgattg ccgaattcag tgatctgatt
     3181 tcttttttct tgaatttttt cgacgggaga ggcattctta taataaggtt ggccatttgg
     3241 atagcgcaat accatcttta atcacactcc taggccacat tatagcaaaa aagcggatag
     3301 aaagaaaagg aatctgagat ggataagtca aaagtaatct atgtgtctgg gtatcgaagt
     3361 ttcgaactcg gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa aaatgtttta
     3421 aaaaaagaac tccatcagtt agcagaagaa gggctggaat gggtacttgt cagtgggaat
     3481 ttgggcgtcg aactatgggc tgtgcaaata gtagcggaat tgaaagaaga atatccggaa
     3541 ctatctcttg gtattttgta tcctttttct gattttggta ataactggaa tgaagggaac
     3601 caagaaaaaa aacaattagc agaacaatta gcagattatg tcgaagctgt tagccatcaa
     3661 ccctaccaat cacctagtca gctaaagaat cacactcgtt ttatacttga gcatacagtg
     3721 ggctgtctgc tgatctatga tgaagaattc cctggaaaaa cgaagtattt ttttgaagat
     3781 gcacagaatt accaagcaga tcatccctac gaaattcggc tgatcagtat ggacgatctt
     3841 caaaatttta gtgaataatc gatattgtgt ttgatttatc aacttttttc ctatacaatg
     3901 aaaaaggtag aaatgttatt acgtaaatga aaactagagg tgtaaatatg gcgaatttgg
     3961 tttatagtcc taaagacatt ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc
     4021 ccgttgaagt tgatgaattc ttagacaata tcatcaaaga ttatgaaact tacagcaaag
     4081 aactccttgc tttacaagaa gaaaacgatc gattgagtgc aaaagtagct caattatcta
     4141 aaactcaagg agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag
     4201 tgaccaattt tgatatctta aaacgcttgt caaatcttga acgtgaagta tttggtaaaa
     4261 agcttgatca acaagcaagt gcagtcaaac cagcacaacc taatccaaat aactacacaa
     4321 atgcagacac aagcttagat gataatgaaa aaacacgtca attttaattt ttgattaatg
     4381 aatttgcggt tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa gtccatgctc
     4441 gcacaggctg tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta aggtactctt
     4501 tggagtaacg gcaggaaaac aagctaaggc ttcggctatg cttaagtatc cttgaaagtg
     4561 ccacagtgac gaagcggctg gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg
     4621 agcaacccaa acaatggtag gggcgctttt tgtctggaag aagaacagga caaaaaggca
     4681 gtataactgc agatagatgg ttaccgcttt gttttttctc cctggaaaag caaagtacag
     4741 aacatggctt atagaaaacc gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt
     4801 tataagaaac aagaaaaaca tttgaaagct atttttcaat acttcagatg aatcgttctt
     4861 attacaaaac aaacttttgt atcaccggtt attcggaatt tgagtaaaag tgtggggtag
     4921 gactgatgtc ccacctcctt tttttataaa taaggagatt gtgtatggaa aaaaacagga
     4981 agttcaacct cgtcgcaact gcggcaagcg ggctagaagc actggttggt aaagaattaa
     5041 gagatcttgg aatcgaatgc caagttgaga acggacgtgc acgttttgaa ggaacaatgg
     5101 aaacgatcgc tacagctaat ttatggttac gtacagcaga tcgagtaaaa atcgtggttg
     5161 gggaattcga tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg
     5221 aagattttct tccactagat gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac
     5281 tatatagcac acctgattgc caagcgatca caaaaaaagc aatcgttgaa cgtctccgta
     5341 cctattatca ccgtccagct tctgtaccac tgacggaaac tggcgcacat tttcagttgg
     5401 aagtagcttt gctgaaagac catgtgatgg taactctgga tacgacagga ccaagtttat
     5461 tcaaacgtgg ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag
     5521 cacttattat gttgacaaat tggcgaaaag accgtccatt ttatgatcct gtatgtggtt
     5581 caggtacgct ttgtattgaa gcagccttaa taggccataa tatcgcacca ggattcaatc
     5641 gtgagtttgc ttgcgaatct tgggattggt tcagtcaaga aatcatggaa aatgttcgat
     5701 cagcagccga agaaaaagca gattatgata ttgaattaga tattactggt tcagatatca
     5761 atggacggat gattgaaatt gccaaagcca atgcagaaga aatcggatta ggctcttcga
     5821 ttacctttaa gcaacaagca gtcaaagact tcaaaacgga taaagaatac ggggtcatcg
     5881 tggctaatcc gccttacgga gaacgtctag gagaagaaga aactgttcgg aaattatata
     5941 aagaaatggg cgatgtattc cgtccattaa aaacatggag taaatatatc ttgactagcg
     6001 acttagcgtt tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg
     6061 gtgcattgcg tacggatcta ttccagtatt ggggaactcg tccgcctaga aaaaaagaga
     6121 aagacgaata atcttactaa ttgattcatt caattggatg tttggaggaa tagggaatga
     6181 aagaaaaaga gtttttacaa gaagtaaaag aaatcaatct tctacaacaa gctttaggaa
     6241 tcctcgattg ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag
     6301 tagacagcta tctttacagc atttattttt caaaaaaaat cggtccaaaa atcaaagaag
     6361 ccattcgcta tttttctgaa catccggaag aattgtcgga agttggaact gccgtttttg
     6421 ccaaagtaaa agaagagtat gatttggaac atcgcgtacc ggaagatctt atgagaaaat
     6481 atacagctgc cgtttcttca gcacacggac aatggcaaaa agctagagaa acacaacaat
     6541 tttctgattt tcaacacgca ttgaatcaaa atattgaatt aacaaaacag ttgatacctt
     6601 actggagaaa agatgaaaag acgaattatg atgttttgct gaatcaatac gaacccggga
     6661 tgactgtcga aatccttgat aaggtatttg atcagcttaa agaaggaatt ctttcgatcc
     6721 gccgtacgct ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat cggagaatga
     6781 cgaaaaaaca acagaagaga tttgtagtga aagtaatcga acaacttggt tatgatttta
     6841 gtcgtggtcg tttagatgac actgtccacc catttatgac tggaattaat ccgaatgatg
     6901 cacgaatcac gacacgatgg aacgaaacag atttcaaaat ggctgttttt ggagtgatcc
     6961 acgaggctgg tcatggtatg tatgaacaag acatcgatga taaatatgcg tatacaccgg
     7021 tttatgaagg tacatcaatg ggtatccatg aatcacaatc gttgtttaac gagatcatta
     7081 ttggaagtag tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag
     7141 gaacattcga tgatattcct tttgacaaat tttatcgttc attgaaccat acaaaatcaa
     7201 gtttggttcg tatcgaggca gacagtttga cttaccctct ccatatcatc attcgttatg
     7261 aaatcgaaaa aatgattttc aatgaagggg tagatgttgc tgaactacct gaaatatgga
     7321 atcaaaaata tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac
     7381 aagacgttca ttggtcaggt gcaagctttg gttattttcc ctcttatgca ttagggtaca
     7441 tgtatgcggc acaattactt cattctttgc agcaagaaat agatgtggaa aatgttctag
     7501 cgtcagacga ttattctcca atcaaaaact ggatgtcaga gcatatccat caatatggtg
     7561 catctagaaa accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat
     7621 acctgatcga ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa
     7681 tttacaaaga tagctgaaat tcatctaatc tatagatgtt ttcagctatt tacttaagaa
     7741 agaagtggaa cgaatggata aattacaagt gatcgctgat tatagtatgg accaattagc
     7801 gcatgatcaa acaggacatg ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa
     7861 aattttggag acggaaccgc aggcagaccg ctttgtgacg cttgcagcag cttacctaca
     7921 tgatacgatt gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt
     7981 attccttcgc tctttgccaa tagtagagga acagatctcg atgatttttt ccattatcga
     8041 aaatatgtcc ttttctaaaa atctctctga agcggccgaa ctatctctgg aaggtaagat
     8101 cgttcaagac gctgatcgta tcgaagcatt aggagcaatc ggcattttgc gtactgctta
     8161 tttcggcggt ggacatgggc acccgatttt tgatcctgaa ctgtatccgc aaacattcaa
     8221 ggacaaagaa aattaccgaa aaggcacgac agtgatcaat catttttatg aaaaactgtt
     8281 cttgctgtct gataaaatga atactgatta cggatatgaa gaagccaaac gaagagaaac
     8341 cttcatgcgt gaattcttag aagaattttt tctggaatgg tctattgacg atgaaaattt
     8401 tactccagac aattggatca aaaataacaa aaaatgatgg aggttgtgac agaagtggtc
     8461 agctccaaga tatttcggat cattttccgt aaaagttact tctggaacac tgtttaccaa
     8521 gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa caatttcaaa aaatagcttc
     8581 tcgtgtttga tgaatattgt aacttgtgat tcggaattgg caggagaaca ccgtttattc
     8641 ggaagaatag gactgtgaca agacttttgt cacagtcctt ttttcgaatt caacaagtaa
     8701 aacttttaag aaatattcag agagtaaaat taaaaattgc tgataaacga agatggaaat
     8761 tttatgtatt tttattggtc tatgcaataa ataaattaaa tattcagaat acaattgatt
     8821 ttaacgaaag ggcttctatc aatggttgtg ttgtatttat ctgcccaggt agagtaaaat
     8881 agatcattat ctaaaaggta attgttcata ctatataagt catttgaaag taaaaagata
     8941 ctgtaaataa aaaatgtggt taaataattc tcataatcag tgtaagcgtt atcaaaaata
     9001 tagcatactt gctttgtaga tgaatgacac gatatatgtt tggtatgttc tgcaagacag
     9061 atgaatatgg aggtggagac aatgtcttcc aaagaactaa caatgtattt agagaaagaa
     9121 ttggatcaac tgaaagaaaa ggggttatac aatacgatcg acatactaga aagtgaaaat
     9181 ggtgcttgta tcatcgtaga cggaaaaaag atgatcaatc tagcatccaa caattatctt
     9241 ggatttgcca atcgcgaaga attgaaaaaa gcatgtattg aagcaaccga aacttatggt
     9301 gtaggagcag gagcagtccg aacgatcaat ggttcgctga aaatccatca gcaattagaa
     9361 gaaaaaatcg cagaattcaa agggacagaa gcagcaatcg cctttcaatc tggattcaac
     9421 tgcaatatgg gcgctatctc tgcagtaatg acaaaagaag atgctatctt atcggatgaa
     9481 ttaaaccatg catctattat tgacggttgc cgcttatccg gagcaaaaat catccgaatt
     9541 aaacatcaag atatgaaaga ccttgagaaa aaagcaaagg aagcgataga aagtaagaaa
     9601 tataaaaaaa tcatgtatat cacagatggt gttttctcca tggatggaga cattgcacga
     9661 ttgccggaaa ttattccgat tgttgaaaaa tatggactga tcacctacgt cgatgatgcg
     9721 cacggctcag gcgtaactgg aaaaggtgca ggaaccgtca agcattttgg attaagcgac
     9781 aaaatcgata tgcaaatggg cacgttatcg aaagctatag gtgttgttgg gggttatgta
     9841 gctggatcga aaacactaat cgactggttg aaagctagaa gtcggccatt cctattttct
     9901 acttcgctaa ctccaggtgc agctgcagct gcattggctt cgattacttt gatgcaagaa
     9961 catccggaac ttgtcgaaaa agtttgggaa aatgctaatt attttaaaga agaattgaaa
    10021 aaagtaggct ataacatcgg tatgtctgaa acacctatta ctccagtcat tttgggagac
    10081 gaaaaagtga ctcagacttt ctctaagaag ctgatcgaac atgggattta tgctaaacca
    10141 atcgtttatc caactgtgcc tcttgggaca ggcaggatca gaaatatgcc gactgcggaa
    10201 catacgaggg aaatgcttga tgaagcagta gcagtttatc aaaaaatagg aaaagaaatg
    10261 gaaattattt aactaaattt agttttttcg tctatttaaa attagagaac atgttcacgc
    10321 atatattgtt aatcatctga agatagtagt aaaaaagatc agggaggatc atcatgaaaa
    10381 aagtattggt gacaggatgt ttaggacaaa ttggttcaga gttgatcaca cggctacgtc
    10441 aagaactagg cagtgaaaat gtcattgcga cagatatacg catgcctgaa gtcattgaga
    10501 aaaacgatat ctttgagatc ctagatgtaa tggattacga aaaaatgcgc agcatagcag
    10561 aacaatatca agtggacaca ttgattcatt tggctgctct tttgtcagct gttgcagaag
    10621 caaaaccaca attagcctgg aatttgaacg tgactgggct ggtcaatgca ttggaagtcg
    10681 ctagagaatt ccagctgaaa ttttttacgc caagttcaat cggcgcgttt ggtccaaata
    10741 ctccaaaaga tcatacccca caagatactc ttcagcgtcc tacaaccatg tatggggtaa
    10801 cgaaggtagc tggagaacta ctatgtgatt attattatgt gaaatatggc gtagatactc
    10861 gtggtgtccg tttccctgga ttgatttctt atgcaacctt gccaggaggc ggtacgacag
    10921 actacgcagt cgatatttat tattcagcaa tcaaagaagg aaagtacact agtttcattg
    10981 atcaaggtac atcaatggat atgatgtata tgccagatgc gatcgatgcc attataaaac
    11041 tcatgaatgc agatcctgat cgattggttc atcgaaatgc cttcaatata tcggcgatgt
    11101 cttttgaccc ggaagagatc aaagcatcta ttcaaaaaat cataccagat tttgaaatgg
    11161 attatgatgt ggatcctgta cgtcagtcaa tagcagaatc ttggccaaat tcactagatg
    11221 tttcttgtgc gcaagaggaa tggggctttt cgccgaaata cgacttagat gtgatgacaa
    11281 aggatatgtt ggaaaaactg aaagaaaaat tggtagagac ggtataaaac aagaaccaca
    11341 tccaaaaagt tttttatact tttgggattg gctatggtat gaaaggggac aagggatgga
    11401 agaatatcaa gccacgccag taaaagaagt aaaaatagaa aatgaattaa aacgtacgat
    11461 gggatttttt actgctttat cgactgttat gggcacagtg atcggtgcag gggtattttt
    11521 taaagccgca agcgtagcag aagttacagg atccgccagt ttgcatatgt tttcttggtt
    11581 tctcggtggt atgatttctg tctgtgcagg actaacagga gctgaactgg cagctgctat
    11641 tccagaaaca ggagggatga ttaaatatat cgaacggatt tacggaaata cagcagcttt
    11701 tttattgggc tgggcgcaag tcgtgattta ttttccagca aatgtagcag ccttgtcgat
    11761 cattttcggt acacagttcg tcaatctatt tggtctttcc cagtcaatga ttgttcccgt
    11821 agctgttaca gctgctgtat cgatcctgtt gatcaatttt ttaggttcta aagctggagg
    11881 ggcttttcag tcgattacgc ttgtctgcaa gcttattcct ttgtttgtga tcgtcatttt
    11941 cggtttgttc cgacaagaag gagtagactt ccaactgttt ccgatccaag caggagaaaa
    12001 tctttcattt ttctcagcct tgggagcggg cttgctagca acgatgttcg catacgacgg
    12061 ttggatacac gtaggaaata tttcgggaga attgaaaaag ccggcgaaag atttgccaaa
    12121 agcaatttct ttaggaatca tcggaatcat gatcgtttat ttattagtaa atgcggtgtt
    12181 cttaagaacc gcatctatcg acggagtcgc aggaaacagc aatgcggcaa gtgacgttgc
    12241 aaaaatgatt tttggcggct tcggtggaag actggtcaca gttggtatct tgatttctgt
    12301 ttatggaacc atcaatggct atacccttac tggaatgcgt ctcccttatg ttatggcaaa
    12361 agaaaacaac ctgcctttta gcaagctctt tgctaaactt catgataaaa ctaaagttcc
    12421 tgtagccgca ggtatcttag agctagtcat cgctattggc atgatgatga tcggtggttt
    12481 tgatacacta acggatatgc tgatctttgt catttggatt ttttatacga tggtctttgt
    12541 tggtgtcatt cttcttcgta aaaaagaacc tgatttgttt cgaccataca aagttccaat
    12601 gtatccgttt attccactag tagcaatcat tggagggact tttatcgtat caagcacgct
    12661 tatcactcag acctttttag cttcgatggg gatagtattg acacttgctg gtatcccgat
    12721 ttatctctat ttaaaacgta gatagctaaa acattcgaat tttattagaa ctgcacttct
    12781 ctgccaaaca aaatatttta tttaaatgag gagtacgtgg aagaaatgtc aaaagtactc
    12841 gttaattttg atcacgtaaa tgtcgttttg tctacgatga aatagcaaag taaaagacag
    12901 aaatcgggct caaccgattt ctgtctttta ctttgatttg cgtaactgaa ctattcggcg
    12961 tacgccgcga taaatattta ataaaaccaa gcaaccagaa atgaaaatgg gaagccaaat
    13021 cagatagaca gggtagccaa gcactttaga caaagcatgt ggagtcattt tatccatttc
    13081 aagaagtgct tgctgccaag tctgaagatt gaagactagc atgaaacccg aaataatcag
    13141 gagaataaat cctaaataat gaattgtagt tctttttctt acataagcga tcgtagcata
    13201 aaggaacagt aaaaaaatag gtacgccaaa aacaaagtac atattcagca tctcgacacc
    13261 ccctttgtgt ttatcttact ttactttgaa agaaatgaga aataatagcc tcgttacttg
    13321 cttgaaacag caaaggactg ctaagataag agagatacaa agaatcaagg aggagtcata
    13381 tggcaagtta tatcgaaccg attcgttttg cgctgattgt ttttccattt ttagccttag
    13441 cgatctcatc tgtttttttt atttatgaat atcgaaagta tggaacattt ctagtaacaa
    13501 gggcagttat tttgtattct ttcgtttttt atttattgtg tgcgtatttt ttggtgatct
    13561 taccacttcc ggcaaaggag acggttgccc aaatgacagg gccaaagatg gagctgcatt
    13621 tatttgcttc atggcaacat ttcagagaca acacggtatt ggtcttgtct gaacctagta
    13681 cctacctgcc ggcaatgaag cagagtgtat ttttggaacc agcttttaat gttttattac
    13741 tcataccatt tggcatttat ttaagatatt atttccggct ttcttttatg aaaacgatga
    13801 ttgctagttt ctgcctttct ctattttttg aattgacgca gctaagtgga ctttatttta
    13861 tctacccaag accataccgc ttatttgatg tgaatgattt agtgacaaat acattgggtg
    13921 gaatcattgg ttttgtcgtt acgccgttat ttacatttat gcttcctaca agaagacgaa
    13981 tggatgaact ttcttatgaa aaaggaaaga ctgtctcatt gactcgacgc ttggtcgctt
    14041 atttgatcga ctggacattt ttatctctta ttaatttggt agcggcgatt ttctttaaaa
    14101 ttgcgttcga ttcaggggca gttacttctc atatctggtg gcttttatta gaagtagggg
    14161 tatactttat gattgtaccg tttataacca atggatggac acttggcaaa aaaattgtgc
    14221 gtatccgttt ggtggaagaa gaccgcgaac gaatttcttt aaaagcattg atgatccgct
    14281 acgggtatct gtactttctt ttttatggtt taagctggtt ggggcagttg cttcagcaaa
    14341 tcatgaattc gacaacgaat acggtgttgc agatgagtgc agtattcatt tctctcggtg
    14401 tcttgttact acaaggtatt tttattttaa atatcttgct atcatttttg agaaaaaaac
    14461 ggattctgtt ttatgaaaaa gctagtcaca catatactgt tagcacgatt ggccaaacaa
    14521 aggatgatga ttctcgaaat tagctgagca aaaacgcttt gtctgtgtta taatgccaaa
    14581 gtcgtgataa tttaaagaga agaggtgcac gaatgaataa ttttgatata ttccgcttca
    14641 aaccgtttat caatgaagcg ttaaccgcaa agggcttcaa acagcccaca gaggtccagg
    14701 aacgtttgat tcctttaatt gcaaaaggaa aaaatatcat tgggcaatct caaactggta
    14761 caggaaaaac acatacgttt ttgttgcctt tgtttgaaaa aatcaatgca gaaaaagagg
    14821 aagtacaagt tgttattaca gctccaagta gggaactagc tgcacagatc tatgaagcag
    14881 ctacacaaat cgctaaattt tctacaccgg agatccgtgt ctcgaatttt gtcggcggta
    14941 cagacaaaca gcgtcaatta aatcgtctgc atcaccaaca gccacaggtt gtagtaggca
    15001 cgcctggtag aatattagac atgatgaatg aacaagcgct gaagattcac accgcatttg
    15061 catttgttgt cgatgaagcg gatatgacat tggatatggg ttttttaaat gaagtagacc
    15121 aaattgcaag tcgattacca gaaaagctgc agtttttagt tttttctgcc actattccag
    15181 aaaaacttcg tccattctta cgaaaataca tggaaaatcc aatcattgaa gaaatcaaac
    15241 caaaagcagt tatttctgaa gacattgaaa attggctgat ttcaacaaaa ggtaaagatg
    15301 agaatcaaat gatttatcaa ctgctgacaa ttggtcatcc ttatttagca atcgtatttg
    15361 ccaatacaaa acagcatgtg gatgaaattg ccgattactt gaaaaaccaa ggcttgaaag
    15421 tagcaaagat tcatggagac atcaccccaa gagagagaaa acgagtgatg cgacaagtac
    15481 agaatctgga gtatcaatat gttgtagcga ctgatttagc tgctagaggg atcgatatcg
    15541 aaggtgtctc tcatgttatt aatgcagaaa ttccagaaga tctagacttc tttatccatc
    15601 gtgtcggacg tactggacgt aatggcttaa aaggaacggc cattacgtta tattcgccaa
    15661 atgatgagca agccattgat gcgatagaaa aattgggcgt ctcatttgaa ccaaaagaaa
    15721 tcaaaaacgg ggaaatcgtc aaaacatacg atcgtaatag acgtgccaaa cgagaaaaat
    15781 caaaagatgt attagatccc acgctgattg gtcttgtgaa gaagaaaaag aaaaagatca
    15841 agcctggtta taagaaaaaa atcgattggg caattgcaga aaaaaataaa aaagaacgca
    15901 aaatcgaacg cagacagcaa agccgtactg ctcgtaaaaa taagaaaaat tcacataaat
    15961 aaaaaacaag aggttgtgac aaaaggcgtg tcacaatctc ttgtttttaa gctagacttt
    16021 ttttcattcc gatacaaagt acttcatctt caataaaagg ttctgtgcaa gtatgatacc
    16081 cttgtttttc ataaaaatct acagccgtca tttcagctac tacataacta gtctgacaac
    16141 cttctgaaac cgcccgtttt tcagcagctt caagcaactg accgcctaac ccttttcctc
    16201 ggtattcttt tgctacgcaa aatcgttccg gcttgaagca agtaggtgtt ttcttttcat
    16261 aacgcaaagt agctactggt cttttttgat caagaaatag ataataggtt tgttgcggga
    16321 tatcttttcc gtcgaattct agttctgcag gaatcccttg ttctaataca aaaacttgat
    16381 atctcaaaaa gcagctagct gcgcgtatcc attcatccgc tccaactatt tttttcatca
    16441 tattcctcct tgtcatgaat agcagagatg tattgattcc tttattctta tcgtaccaaa
    16501 aatcgtggag tatgaccaaa aggaaatgaa aataaaaatt gagatattga cagtgatcaa
    16561 tagaatgcgt atactaatta agtacgaaaa ggatatgatt tgttttttaa gactcacaga
    16621 aaattctttc ttggctgaaa aaagaatagt cactaatcaa attgctccct ttttctattt
    16681 tggtagaaat accgacgcag ctcagcgtta tcgagtttaa gaggtaatga ttacacatca
    16741 ttgcaatcaa ggtggtaccg cgagttttcg tccttggctg tgatggtgtt tttttgtatt
    16801 taatttatag aaagcaggta atgacatgaa acaattaagt agtgcacaag ttcgacaaat
    16861 gtttcttgat ttcttccaat ctaaaggaca tacgattgaa cctagtgcgt ctttgatccc
    16921 cgtgaacgat ccaaccttat tgtggatcaa ctctggtgtt gcaacattga aaaaatattt
    16981 tgatggttca gttgtgccag aaaatccaag aatcacaaat gcccaaaaat ctattcgaac
    17041 aaatgatatc gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg agatgctggg
    17101 taatttttcg attggtgact atttcaaaaa tgaagccatc cactgggcat gggaattttt
    17161 gactgatcca aaatggatgg cattcgatcc agaaaaactt tatgtaacag tttatccaaa
    17221 agatacagaa gcaaaacgca tctggcatga agaagttggc ctgccactag atcacattat
    17281 tgatgtagaa gacaacttct gggatatcgg tgcaggccca tgtggtccgg atacggaaat
    17341 tttctatgac cgtggagaag cttataacga tttagcagaa gacgatcctg aaaactatcc
    17401 aggaggagaa aatgaacgtt atttagaaat ctggaactta gtattttcag aattcaacca
    17461 taagcctgat aacacatttg aaccacttcc tcataaaaac attgatacgg ggatgggatt
    17521 ggaacgcatg gtctcaatcg ttcaagatgc gtcaacgaac tttgagacag acttgtttat
    17581 gcctattatc cacgccgtag aaaagctaag caaacaagta aaatatggtg aaaacccagt
    17641 aacagatatt tcttacaaag taatcgcaga tcatattcgc gccctttcat ttgctattgg
    17701 cgacggtgca ttgccatcga atgaagggag agggtatgtc ttacgccgtt tgcttcgtcg
    17761 tgctgtcatg catgggaaaa aattaggtat tgaacaagca ttcttatatc gtttagtccc
    17821 tgttgttggg gaaatcatgg taagttatta tcctgaagta cttcaacaaa aagaatttat
    17881 tgaaaaagtt atccgtacag aagaagaacg tttcaatgaa acaatcaatg aaggtttgga
    17941 aatcttaaat caagtgatcc aagaagtaaa aaacgaaaac gggacgactc tagcaggaaa
    18001 agacatcttc aaactatacg acacatatgg cttcccggta gaattaacgg aagaggtggc
    18061 agaagatgaa ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg atgcacaacg
    18121 cgaacgtgct cgctctgccc gcagtactga aaaatcaatg ggggtacaat ctgctttatt
    18181 gacagatatc aaagtagaaa gtaaattcat tggatacaca gaacaagaat cagatagtca
    18241 attattggtc atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag gtgaagctca
    18301 aatgatcttt gatcaaacac ctttctatgc agaaatgggt gggcaagtag cggatcaagg
    18361 atggatcatg aatgaagtag gcgaaatcgt agcagttgtt acagatgttc aaaaagcgcc
    18421 taatggtcaa tttatgcaca ccgtagaagt aaaagcacca atgagtgaag gacaaaccta
    18481 tcatctattg atcgatcaat cgatgcgcaa tcggatcatc aaaaaccata cagcaaccca
    18541 tctactgcac aaggcattga aagaagtact tggcgagcat gcaaatcaag ctggttcttt
    18601 agtcgctcct ggacatttac gattcgactt tacccacttt ggtcaaatca cttcagatga
    18661 attagctgaa atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc cagttgaaac
    18721 aattgaaaca gatatcgata cagccaaaaa catgggtgca atggctttgt ttggtgaaaa
    18781 atacggacat gacgttcgag tagtaaatat tggcggctgg tcaatcgaac tgtgcggagg
    18841 cacacatgta gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg aatcaggaat
    18901 tggtgcaggc gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg aactacttga
    18961 agaagaagaa aaacaactaa aacaagtagc aacaattgtt aaatcacctc aattgaaaga
    19021 agttgtttca aaagcggaac aattgcaaca gcaagtaaga gagctacaaa aggaaaatga
    19081 acaattagct agcaagttag cgaatcaaca agctggtgat gtattcaaag atccagaaac
    19141 gatcaatgga aaaacaatca ttgcagcaca agtaaaagtg aaagatatga atcagctgcg
    19201 tcaattagct gaccaatgga aacaaaaaaa tatttcagat attcttgttt tagcaaacga
    19261 acaagaagga aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg aagctggact
    19321 aaaagctggc gacttgatta aagcaatcgc accgaaagta ggcggtggcg gtggtggacg
    19381 tccagacatg gcacaagctg gcgggaaaaa tcctgctggt attccagatg cacttaaagc
    19441 tgcgaaagaa tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa acaaaagttc
    19501 aaacattgaa cgaaacatga ttatgggggc gaagacaaac aagtcttgcc ctcatacttt
    19561 tttcaattgg atggcgcatc taatcaaatg gtcggattcg gatggtcttt agttgacgaa
    19621 agtgacagaa atttttacga ttagtataaa taaaaagggg tgaaaaaatg aatagtagaa
    19681 ataagacctt tcgactagtc ttacgtgcga tcttgttagc aatcatcatc gtacaagcga
    19741 tggttccttg gctggggttt attccattag gattcattag tttaacaatt atccatatca
    19801 cagtaattat tgctgcagta gtattaggcc caaaagatgg catggtgatt ggcttgtttt
    19861 ggggaatcgc tacaattgtt cgtgcctatg ccatgccaac aacgccattt gatacattag
    19921 tgttcacgaa tccgattatc tcggtagttc ccagagtact tgttggctta gtagcaggct
    19981 tggttttcca ctggatttat caaaaaaata aatcaataac gatcagctca gcctttgcag
    20041 gggtactggg ttcattggtc aatacagtct tagtgttagg attcatggga ttattttata
    20101 caggtgcaac ggcagatgcc tatggtgtag actctgctct tttatttaaa accttagcag
    20161 gggttgcagc aatcaatggg ataccagaag caataggtgc gggaatcata acaccattac
    20221 tagcaaaagc gttatttgca gcaacaccgt tgaaaccaga ataatgacca tcttttaata
    20281 gtaataggac gcacgataga tagaaaaagg aagagaggag aggaaaacaa tcgtcctctc
    20341 catcttttta cctgagcaac aagcaaacta gaaggggtaa aaaacttggc taagaaacaa
    20401 gaagaaaaaa agaaacaaat accgcaacag cggacaaaaa aaggagtact agcgggtatc
    20461 ttaggattgg ttacgaatct gatcttgttt gtctctaagc taatgatcgg gcttttttca
    20521 ggaagtgtct ccattatggc tgatgcagtg aacagtctat cagatactgc ttcatccata
    20581 ttgactttga tcggatttaa aattgctgca aagccagcag atcaggaaca tccttatgga
    20641 cacgaacgct tcgaatatat cagtgggtta tttgtttcga tcatcattac ttatgtagga
    20701 tttcaatttt tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt ctctttaaca
    20761 cccatcgtct tttttgtcct aatcatttcc atctttttga aatttgcaca aggcaaaatg
    20821 tatcaggtta ttgcgcaatc cattgattct gaaacattga gagctacagc aacagatagt
    20881 tataatgatg tctttactac actggctgta ttgctttcag cagcaatcga atgggtaact
    20941 ggatggcgta ttgatggata tataggtttt gctttagcat tatatatcct ttatagtgga
    21001 ttgacgatgc ttcgaaattt tgtatacgaa ctattaggaa gtcgcccaac gccagcagag
    21061 attgaagaaa tggaagagca tttaaatgct tgcgatgata tacttggcta tcatgattta
    21121 ttggtgcata actatggacc caataaacgc tttgcatcag tccacatcga agtagatgaa
    21181 tcaatgaatt tgaatgtggc tcataagatt attgatctga tagaaaaaga ctttaaaaag
    21241 tctttggatg tggaacttgt ctgtcacttg gatccaatgc ctgttgataa cgaaaaatac
    21301 tataatatct tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg gcttaatatt
    21361 catgatttta gagtaattca tcaaggaaaa acgctgcaat ttgatgtagt ggtccctgag
    21421 aaattccagt ttgttgatac agaactagaa agaatcttac gtagtcaaat agaagaaaaa
    21481 atcggagatt accaattgga aattacgttt gatcacaatt atcttttata ttaaatgaga
    21541 aactataaaa agactcctta aagtttttaa tttaaagagt ctttttttct tttaattgtt
    21601 tatggagagc ctgacgtgca agattatctg ctcctttatt tttgttttcg ggcaaccatt
    21661 gcaggatcaa taaagaaaat tcggatagaa gttcttggat tctttgaagg tagggaagga
    21721 agagctcgtt cttcgttgcg tttttgtcga ccgtagaaac tagtgttttg ctgtctgaaa
    21781 aacagaaaat cgtttcattc tgtaagtcgt ttttctttag aaattctaaa gtttttaaaa
    21841 agacttcgaa ctctgcttga tggtttgtcc ctatagaaag cgggatagat agctgttctt
    21901 gtttaccttg atagacaatc aagattccac ctccactagg accagggttg ccttttgtag
    21961 aggcatcaat gtatactttt aacataaaaa gagattctcc tttttgttca aactaatgtt
    22021 atggtaagat tatatcatta ctgaatcggt gagaggttag agaatgaaaa aaaatattcg
    22081 atggcagcct gaactggcac atacgatcat ctattggtca ttgaccttga tcattctgtt
    22141 ttttagtctg attctttctt tagaaaacac aggtccttat tggaaaagca attttgtttt
    22201 aggtattttt cttgtttttc tatttctagg tttgcggcgt tcttttattt taaaatcaaa
    22261 ctctgtgatt atacgttatg ctgctttttg gaaagaccga gaaatatctg tcggacagat
    22321 tcgatctttg acgccaacta atactggggt gcaaatgatg ttgcaagatg agaaagcccc
    22381 atctgttttc atgatgcgaa aaaaagatca agtagtcctt tttgatcatt ttgaaaaaaa
    22441 cggacccaaa tcagaatcaa ttccttatcc ttatacgaaa aaaagaaaaa gctaatttta
    22501 aaaatcagaa cctaaaaaaa cgaattttta caggaaaaga aaagagtgag acgtacggtt
    22561 catcccgttt agcgtctcac tctatttgtt tatctattca tgcatactag tatcggtagt
    22621 ctgaacgttg cctttcttga catgggcagc ttgtgttcct cgattccctt cgatgatctc
    22681 aaattcgacc acttgatttt cttccaaagt tttgaatccc tcttcttcaa tcgcagtaaa
    22741 atgtacaaaa atttcttctg tgtcatcata actgataaag ccgtatcctt ttttgttgtc
    22801 aaaccatttt acaattcctg tggtcaaagc actcacgacc tttctaaagt ctgatcatag
    22861 ccttcattat aaagagtatt tataagtacg tcaaataaat taaaaacctg taattttatg
    22921 ttttttttct gaataaaaaa aggtataatt ggcatatctt aacaaagaag atattttgtt
    22981 gaataaagtt cgtgaaaagg ggtaatcaaa tgaagaatga tctgcaaatt tcacaagaaa
    23041 cagagctaga accgattgtt gaagtggcta gtaaattaga tcttaaggaa gacgatttgg
    23101 aactgtatgg gaaatataag gcgaaaatca atttttctgg aatcaatcga tcaaaggata
    23161 acgctgaggg gcatttgatc ttagttactt ccatcaatcc aacaccagct ggcgaaggaa
    23221 agtcaacgat cactgtaggg ttaggagacg cgctcaataa aatcaataaa aaatctgtta
    23281 ttgcattgcg agaaccctca cttggtcctg tgatgggaat caaaggtggt gctgcaggcg
    23341 gaggatatgc acaggtattg cctatggaag atatcaactt gcattttact ggtgacatgc
    23401 atgctatcac aacagctaat aatgcattat ctgctctttt agataatcat attcatcaag
    23461 gaaatgaatt gaacatcgat gcccgccgag tgatttggaa acgggtagtc gatctcaatg
    23521 atcgtgaact gcgtaaagta attgttggct tgggtgggcc aatccaaggt gttccaagag
    23581 aagatgggtt tgatatcaca gttgccagcg aaatcatggc tatcttgtgt cttgcggcag
    23641 accttgaaga tttaaaggct cgtcttgcaa ggatcgttgt cggctataca tttgaccgtc
    23701 agcctgtgac tgctggagac ttgaaagcag aaggcgctct ggcattactg ctgaaagatg
    23761 caatcaagcc gaatcttgta caaacaattt acggaacacc agcttttgtc cacgggggac
    23821 cgtttgccaa tatcgctcat ggctgcaata gtattttagc taccaagaca gcattgagat
    23881 tagcagatta cgttgtcaca gaggcaggat ttggtgcaga tcttggcgga gagaaatttt
    23941 tagatatcaa agtacctaac ttgaaaaaag cgccggatgc tgtggtgatt gtcgccactg
    24001 tccgtgcatt gaaaatgcat ggcggaatga aaaaagatga acttaaaaat gagaatcttg
    24061 atgctttgaa agtaggtttt gcaaatttaa agcgccatat acgtaatatg gaacaatatc
    24121 agctacctgt gatcgtcgca atcaatgaat tcgtaacaga tacagacaat gaactcacac
    24181 tgttggagca cttgtgtgaa aatcagggga ttcttgccaa gagagcgagt gtatgggcag
    24241 atggagcaga aggcggcgtt gatttagcaa aagctgtagt acgattgatt gatagaaaag
    24301 aagctaatta caagccatta taccgcctag aagaaacgat acaagaaaag acagaaacaa
    24361 tcgtcaagaa aatttacgga ggaaatggtg ttgttttcag caaaaaagcg caaaaacaaa
    24421 tagaagagtt tacgaagaat ggatgggaca aactgccgat ttgtatggca aaaacacagt
    24481 attcattttc tgatgatcct tcactgctag gtgcaccgga agatttcacc atcacgatcc
    24541 gcgaaatcat cccaaaatta ggggcaggat ttttagtagc cttgactgga gatgttatga
    24601 cgatgcctgg tttgcctaaa aaacctgctg cattgaatat ggacgtgaca aatgacggag
    24661 aagtgttggg attgttttaa gcttgattat aatctaccat cgaaggagct gtattttgac
    24721 tacagcttct ttttttagac gatagggaat taattttgtt tacactagga tcattctttt
    24781 tctttattgt attttgagct atactaaagc ataataggag ggtatgatga aaacgacatt
    24841 tataagaaaa accggttttt acggtatggg tagctcgatt cagctaagaa tcaacaatga
    24901 aaaacgcagt gttctcagtc atgaacaaaa gataacgctt gatctggaag tgccctttac
    24961 gatgcaggtc accttttatt ggctaaaaag ccccgtttat acagtgaaag aagcaaagcc
    25021 tgcttatgtg atcacaatga attacttgat tcttcaaatc tatccgtttt tatttctagc
    25081 aactgggttg ggggccgttt tcctacaaaa tttatttttc agtttttttg cagttattgt
    25141 gttgattgcg ttctttttct atataaaaaa ccgggcatat ttgatcaagg aggaaaatta
    25201 tggcaaactc tgatcttttt ttaatcagtt tcaatcgaat cgaaaaatgg atgagagacg
    25261 agatgggaaa tgcacgaaat atgggattta ccgaacttgt cagacgccta gctcaaagaa
    25321 aacaattaat gatcagaaaa tacgaagatg atcttttaca gcttgctcag ttaaggaatg
    25381 cgatcgtcca tgatcggatc gcagtcgatt ttgtcattgc agagccaaat gattggacag
    25441 tcaagcgaat ccagctgatc gaacaagaac tgattcagcc tgaaaccgtg ctgcctcgat
    25501 ttgcaaaacg tgtaacaggt tttgaacaag atcttccgtt acttgaactg ttaaagatag
    25561 tagcagaaaa aaggtattca caatttccgt tatacaataa aggaaaattt gtagcattga
    25621 ttactttacg taatatcggg ttttggctag caaaagaaag ccaaaaaggt ccggttgatt
    25681 taacaaataa acgagcgttg gatttgatca tccaaaacgg aaagtacaca aattatcatt
    25741 ttgttccagc taaaacacat atttacgagg ttgaagcgat gtttcgtgag caagggactt
    25801 tggaagccgt acttatcact aaagatggga atcccgatgg taatctgctc gggatcgtac
    25861 gacctcgtga tatttataaa caaatagaaa aggattgatt ttttgttaat tatttattgg
    25921 atattcagcg ctttgctagt aggagtggat cagttggtca aatggtggat cactgataat
    25981 ctagcgcttg gagagacgaa aaatcttata ccaggtattt tgtcactgaa tcatattcga
    26041 aacactggag ccgcatggag tatgctagaa gggaagatgt ggttctttac tatagtgaca
    26101 ctcattgcag tcgttgtgat tctcacattg atgattaaaa atcgtgaaaa aggtaatcgc
    26161 tggttcatga ttggattgag tttgattcta gctggtgcga ttggcaattt tatcgaccgt
    26221 attcgtcttg gctatgtagt agatatgttc cagacagatt ttatgagctt tccgattttt
    26281 aacgtagcag atgtgacact tgtatgtgga gtgatttgta tgttaattta tattatttta
    26341 gatgaaaagg atcaaagaaa aaaatgacag aacttaaaac gacgattcac catgaatctg
    26401 gtaggatcga taaagtattg actaatttat tcaccgatta cagtcgttcg caaattcagc
    26461 tgtggctgaa aaatggagcg gtttctgtca atggacagcc agtcaaagca aattacaaag
    26521 taaaacaagg agacgagatt actgtcttga tccccgaacc agaaacatta gatgtggtcc
    26581 cagaaaatat cccgttgacg atcgtttacg aagatgaaga tgtagcggtg ataaacaaac
    26641 ctcaaggaat ggtcgttcat ccttcagcag gtcatacgca tggcacaatg gtcaatgcgc
    26701 tgatgtacca tatcaaggat ctttctacga tcaacggcgt tattcgtcca ggtattgttc
    26761 accgaatcga caaagatact tccggactgc taatggttgc caaaaatgac caggcacacg
    26821 aatcattagc tcagcaatta aaagataaga cctctttgcg caaatatgtt gctttagtcc
    26881 atggagagat tcctcacgaa aaaggaacca tcaatgcgcc aatcggccgt tcgaaggaaa
    26941 accggaaaat gcaggcgata agagaagatg ggaagtcggc tgtcacacat tttactgtat
    27001 tggaacgttt cgaaggattt actctggttg aattaaaact agaaaccgga agaacacacc
    27061 agatccgtgt gcatatgaag tatatcggtt atccgttagc tggagatcca gtatacggac
    27121 cgaagaaaac tttgaaaggc aatggtcagt ttctccatgc gcaaatgtta ggttttacgc
    27181 acccgactac aaacgaaaag atgatctttg aagcacctct tccagaagtt tttgaaaaaa
    27241 ctttagaaaa attaagaaaa aacattgcat tttaatttag ataacggtat agtaattaac
    27301 aacgaaagca taaaacttaa tagaattcct ttaagtttag tcccgtgagg ctaagaaggg
    27361 tgggagcaag gatacgtatg gtgcgccata agtagattgc tcattgacat agtatagcta
    27421 tcagtgtatc agtatacgct cagtctaaac attcctcctg tcctcttagg gcaggagttt
    27481 ttttatgcaa aaaagtaggg ggaatgaaaa tgcaagcaaa agaagtagta gatcaggtaa
    27541 ccatgaagcg agcattgact cgaattactt atgagattat cgagagaaac catagtatcc
    27601 aagatatcgt attggtaggt atcaaaacac gaggaatcta cattgcatca agaattgccg
    27661 aacgcctgaa gcagctagaa gatatcgata ttcctgtagg agaactggat atcactttat
    27721 accgagatga caaaaaagaa aatccagaag aaccggaact tcattcttct gacatcccag
    27781 tttccttaga aggaaaagaa gttattttga ttgatgatgt tttgtataca ggacgaacga
    27841 ttcgagcagc aatggacgca gtaatggatt ttggcagacc aagaaaaatt tcattagcag
    27901 tcctggtcga tcgtgggcat cgagaattgc caatccgtgc agattatgtt gggaagaaca
    27961 ttccaacatc tcgggcggaa gagattttag tagagatgca agagcttgat ggacaagatc
    28021 gcatcatgat tttgaaagag gaagattaga ggagagagaa acatgtcgga gaaaaaagga
    28081 tttcataatg atgacgtggt gttagatatc cacgatcgcc caaccccagg acattgggta
    28141 ggattaagtt tacaacactt gttcacgatg ttcggagcaa ccgtgttagt ccccatctta
    28201 gtaggtatcg atccaggtat tgcgctagtc agttcaggac taggtacatt ggtgtactta
    28261 tttactacta aaggaaagat acctgcgtac ttaggaagca gcttcgcttt cattgcagcg
    28321 atgcagcttt tgatgaaaac agatggttat ccagcgattg cacaaggagc agtcacaact
    28381 ggattagtct atttgattgt tgcgctgatc atcaaaaaga tcggttcagc atggctggat
    28441 aaaatcttgc ctccaatcgt cgtcggtcca gtcatcatgg ttatcgggtt gggacttgcg
    28501 gcaaatgcag caacaaatgc tatgtataat cagggcacgt atgatttcaa gtatattgct
    28561 gtagcgctga tcacattagg attaacaatt ttcttcaaca tgtggctaag aggttttctc
    28621 ggattgattc cgattttact aggtattatt agcggataca tcgtagcctt gctgtttgga
    28681 atcgtggata ctaagccaat cgtagacgct gcctggtttg ctgttcctaa ctttgagatt
    28741 ccctttgtac aatatgaacc aaagctatat cttggagcaa tcacaacaat ggcacccatc
    28801 gcttttgtga cgatgacaga gcatatcggc catcttatgg tattgaataa actaactaaa
    28861 cgtaatttct ttgaagaacc gggtcttcat aagacgttga caggtgatgg attagcacaa
    28921 atcgtggcag cctttgtcgg aggtcctcct gtaacaagtt acggtgaaaa cattggtgtt
    28981 ctggctatca cacgtgtcca tagcgtgttt gtgatcggtg gagctgcagt atttgcagtc
    29041 attttaggat tcgtaggaaa attaagcgct ttgattttaa gtatccctgg accagtcatc
    29101 tcaggaatca gttttatctt atttggagta attgccgcaa gtggaatgaa gattttaatc
    29161 gagaacaaaa tcgattttga taaaaagaaa aatctgttga ttgcttcagt tatcttagta
    29221 gtaggaattg gcggactcgt ctttgaagta ggcacattta cattgtcggc aatggcatta
    29281 gcaactgtac ttggcatcat tttaaatctt atcttaccag aaacttcacg aagcgaagaa
    29341 aactaaggag gaaacattat gatcatcaaa tcagaacgta tcagcttaaa gcatttattg
    29401 accgttgagg cacttactga tcaagaagtt atgggcttga tccgcagagg gcaggaattc
    29461 aagcaaggtg ctaattggac tccgcaaaag cgccagtact ttgctacaaa cctctttttc
    29521 gaaaatagta ctcgcacaca taaaagcttc gatgtagcgg agaaaaaact agggctagaa
    29581 gtgattgaat ttgaagcaag cacaagctct gtccaaaaag gggaaacact atacgataca
    29641 gtactgacga tgtcagcatt aggcgtagac gtagcggtta tccgtcatgg tgatgagaac
    29701 tattatgacg aactgataca aagtaaaaca atccagtgtt cgattatcaa tggaggagac
    29761 ggaagcggac agcatccaac ccaatgcttg cttgatttga tgaccattta tgaagaattt
    29821 ggtcattttg aaggtctgaa agttgccatc gtaggtgata tcactcactc tcgcgtagct
    29881 aaatccaata tgcagatgtt aaaacgtcta ggcgcacaag tattcttctc aggtcctaga
    29941 gaatggtacg acgatgaatt cgaagtgtac ggacattata tgccattgga tgaactacta
    30001 gatcaagttg atgtcatgat gatgcttcgg gtgcaacacg aacgccatga tggaaaagaa
    30061 agtttctcaa aagaaggcta ccatcaggaa catgggttaa cagttgagcg tgcgaagaag
    30121 ttgcaaaaac atgcaatcat tatgcaccca gcaccagtta accgtgatgt agaattagct
    30181 gattcattag tggaaggatt acagtcgcgg atcgttcagc aaatgagcaa tggagtattc
    30241 gtcagaatgg caatcttaga agcagtgcta aatggaaaag cgtaattata ggaggatgaa
    30301 aaaatgaaaa cactcattaa aaacggtcag atcaatacac gaaaaaatat gacgacacca
    30361 gcagaaattt ggatagaaga tgggcgaatc aaagcaatcg gtacaggatt ctcagaagcc
    30421 gaatttgatg aagtgtttga tgcaaaggga caattgatca cacctggttt agtggacgtc
    30481 catgttcatt tgagagagcc aggatttaca tacaaggaga cgatcgaagc tgggactcgc
    30541 tcagctgctc gtggcggttt cactactgta tgcgcgatgc cgaatttaaa tcctgtacct
    30601 gatacggcag aaaagctgag acaagtatat gacatcatta gaaaagatgc agttgtgaag
    30661 gtattgcagt atgcgccaat cacagaaaat ctgcgaagtg agaaattagt tgatcaagaa
    30721 gcactgattg aagaaggtgc ttttgctttc acaaatgacg gtgtaggtgt ccaaacagct
    30781 gggacgatgt atctggctat gaaagaagct gcaaaaaaca ataaagccct agtagcccat
    30841 acagaagatg aatctttatt atttggcggc gttatgcacg ctggcaagaa ggcagaagaa
    30901 cttggtcttc ccgggatctt aagcgtaacc gagtcttctc aaattgctag agatctgttg
    30961 ctagctgaag caactggtgt tcattaccat gtttgccatg tttctactaa agaaagtgtc
    31021 cgtgtaatca gagacgcaaa aaaagcagga atccacgtaa cagcagaagt ttcaccccat
    31081 catcttattt tgatcgacga agatattccc gaagatttcg gcttttggaa aatgaacccg
    31141 ccgctaagag gacgggagga cagagaagct ttgatcgaag gcttgcttga cggtacgatc
    31201 gattgcatcg ctacagacca tgccccccat ggattagaag aaaaaagcca aagtttcatg
    31261 aaatcaccat ttggtatcgt aggaagcgaa acagcgtttc aactgatcta tactcatttt
    31321 gtagaaactg gtcgattcac gttggaacaa gtgatcaatt ggttggcagt taaaccggct
    31381 gagatttttg gtttgaatgc aggaacgcta acggttggtg cccccgctga tgtagcagtt
    31441 tttgatatta cacaaacttg cacgatcgat aaagaagact ttttatcaaa aggggagaat
    31501 acacctttta tcggctggaa agtaaaaggc gaaacacaaa tgacttttgt caacggaaaa
    31561 cttgtgtggc agaagggaga ataaaagtat ggaacgtttg cttatattag aagatggaac
    31621 agttttcgaa ggaaaggctt ttggtgcaga tgctagtgtc ttcggagaaa tcgtttttac
    31681 aacaagtatg actggttatc aagaaactat cactgatcaa agttttaacg gtcaaatcat
    31741 tacatttacc tatcctatgg tgggtaatta tggggtaaat agagatgatt atgaatcgat
    31801 ttctccaaca tgcaaaggag tagtagtaaa agaacatgca cgtgtagctt caaactggcg
    31861 aaaccaaatg acgctagatt catttttgaa acaaaaagga attcctggaa tatcaggtat
    31921 cgatacgcgc gcccttactc gcaaaatccg tgaagtcgga acgatgaaag ctagtatagt
    31981 tgatgctgga gattccttcg aacatgcatt tgatcagctg aaagcaaccg ttctagcaac
    32041 caatcaagtg cagcaagtct caactaacaa accttatcca agtccaggaa caggtcggaa
    32101 agtcattgtc attgacttcg gcttgaaaca tagtatttta cgagaattga gtaaaagaaa
    32161 ctgtaacctc acagtacttc cctataacac agatgccgag acgattttag cgctatcacc
    32221 agacggagtc atgctgacga atggacctgg tgatccaaaa gatgtgccag aagcaatcga
    32281 aatgatcaaa aaaatccaag gaaaagtacc gatttttggt atttgtctcg gtcaccagtt
    32341 atttgcttta gccaacggtg cagataccta taaaatgaaa ttcggacatc gaggattaaa
    32401 tcacccggta cgagaagtag cgaccggaag aattgatttt acttctcaaa atcatggcta
    32461 tgcagtcgat gaagccacga ttgatccaga aaaactcatc gtaactcatg tagaagtaaa
    32521 tgacggaaca gtagaaggag tcaaacacag agattatccc gctttcagtg tgcagtttca
    32581 tccagatgct gcccctggtc cgcatgatgc actacattta tttgatgaat ttatggaaat
    32641 gatggacgca ggaaaggagc agtaaacaga tgccgaaacg tacagatatc aagaaaatta
    32701 tggtgattgg atctggtccg atcatcattg gtcaagccgc agaatttgac tacgcaggaa
    32761 cacaagcctg ccttgcctta aaagaagaag gatatgaagt ggttttagta aattctaatc
    32821 ctgcgacgat catgacggat aaagaagtgg cagatcaagt atatatcgaa ccgatcacac
    32881 ttgaatttgt atcaaggatt cttcgaaaag agcgtccaga tgctctcctt ccaactttag
    32941 gcggacagac aggcctcaat atggcaatgg agctggcagc atcaggtatt ttagaagaat
    33001 tgaacataga acttctagga acaaagcttt ctgcgatcga tcaagcagaa gatcgtgatc
    33061 tattcaaaca gttgatgaaa gaattgaatc agccaatccc cgagtctgaa atcgtcaata
    33121 cagtagaaga agctgtagat tttgcaaata aaatcggcta tcctgtcatc gttcgtccag
    33181 cctttacttt aggcggaact ggtggaggta tgtgtgaaaa tgaagaagaa ttacgtatcg
    33241 tatcagaaaa tggattaagt ctttcccccg ctacccaatg tttgattgaa cgaagtattg
    33301 caggttttaa agaaattgaa tatgaagtga tgagagattc ggctgataat gcaatcgttg
    33361 tctgcaatat ggaaaatttt gacccggtgg gtatccatac aggtgattct atcgtatttg
    33421 cccctagtca aacattgtct gaccatgaat atcaaatgct tcgtgatgct tctctttcga
    33481 ttattcgagc acttaagatc gagggaggct gtaatgtcca actcgctctt gatccacata
    33541 gttttaatta ttatgtgatt gaagtcaatc cgcgtgtttc tcgttcatct gcgttagcaa
    33601 gtaaggcgac tggctatccg atcgctaagc tagctgctaa aatagcgctt ggattaacat
    33661 tagacgaaat gaaaaatcct gttacaggaa cgacttttgc agaatttgaa cctgcattag
    33721 attatgttgt agctaaaatt cctcgctggc cgtttgataa gtttgaaaac ggagaacggc
    33781 ttttaggcac gcagatgaaa gcaactggtg aagtgatggc tatcggtcgg aatatcgaag
    33841 agtctttatt gaaagctgtg cgttctttag aaatcggtac acaccatatc gaacttcctg
    33901 agttggcaga catcacggac gatgacctaa tccaaaaagt ggttcgagca caagatgatc
    33961 gcctgtttta tgtagctgaa gctattagaa gagggtacac catagaagaa ttagcggatt
    34021 tgacaaaaat cgatttattc tttttagata aactgttgca tatcgtagaa atcgaacagc
    34081 agttgaaaca gcagccgttg gatgacaaga tattgaaaac agcgaaacaa aacggtttta
    34141 cagacaaaaa agtggcggag ctgtgggaaa taaacgaaga agccgttcgc cagatgagaa
    34201 aagaaaatga tttgattcct gtatataaga tggttgacac ttgtgcagcg gaattcgaat
    34261 caaaaacacc ttatttttat agttcctatg aatatgaaaa tgaaagttca cgatcagaaa
    34321 agccctcaat tctagtactg ggatctggtc ctatccgtat cggacaaggg gttgaatttg
    34381 actatgcgac tgtgcattct gtcaaagcaa tccagcaggc aggatacgaa gcaatcatca
    34441 tgaacagcaa tcctgaaaca gtttcgactg acttttctat ttcggataaa ttatattttg
    34501 agccattgac cttcgaagat gtgatgaatg tgatcgagct agaacagccg ataggagtaa
    34561 tcgttcaatt tggtggtcag acagcaatca acttagccga acctttgact gctgctggtg
    34621 taaaaatctt agggacaacg atcgaagact tggatcgtgc ggaaaatcgt gatttattcg
    34681 agcaagcgct gaaagagttg gatattcctc aacctttagg ggatactgct acaagtaaag
    34741 aagaagccgt ggttatcgct tcgagaattg gttatccagt tcttgttcgt ccaagctatg
    34801 tattaggcgg acgtgcgatg gaaatcgttg aaaaccagcg agatttagaa gattacatgg
    34861 aacatgctgt gaaagcatct ccagaacatc cagtactagt ggaccgttac ttgcttggtg
    34921 gtgaatgcga agtggatgcg atttgtgacg gtgaaacagt actcatccca gggatcatgg
    34981 agcatattga gcgtgctggt gtccattcag gggattcaat ggctgtgtat ccacctcaaa
    35041 cattatctga atcgatcaag aaaacgatcg aagattacac gatccgctta gcattaggtt
    35101 tgaattgtgt cgggatgatg aatatccagt ttgtcatcca tgaaaatgag gtttatgtga
    35161 tcgaagtcaa tcctcgagca agtcggactg ttccattctt aagtaaggtg acaggaattc
    35221 caatggcaca aattgcgacg aaagctattt tgggagagaa actaaagaca cttggttatg
    35281 aaaacggact ttatccagaa acaaaagaag ttcatgttaa agcacctgtc ttttctttta
    35341 cgaaattgca gaaagttgat acttacttag ggcctgaaat gaaatcgact ggagaagtaa
    35401 tgggatctga cagacaatta gacaaagctt tgtacaaggc attcgaagct tctggattac
    35461 acttgcctga atatggagcc gtattattta cgattgcgga cgaaacaaaa gaagaagcac
    35521 tagaattagc taaacgtttt acagaaattg gctatagtct gataggaacg aaacatacag
    35581 ctgcttattt cgaaaaaaat ggtctggtcg ttgaaacagt agcgaaaatt tctgaggaag
    35641 cagcagaaaa aaatgtagtc gacttaataa gagatggtaa aacacaagtg gttgtcaata
    35701 cgattgataa agatcgggga aatgcttcta aagacggttt catcatccga agagaagcag
    35761 ttgaacatgg tattccattg tttacttctt tagacacagc agatgcgatc gttcgagtga
    35821 tggaatcccg tgctttttca actaacccta tttaacggcg agcacatgtt aagacaaaaa
    35881 gacgaaaaac cgttgcagga ggggacagat gaaacaagaa atgatggaaa tcgttcagca
    35941 aaaacagctg gcaccaaaga tttttgaaat gacattgaaa ggtgatctag taaaagaaat
    36001 gcagcaacca ggacagttct tgcatattct cgtgccgcgt gcagacttgc tattacgccg
    36061 accgattagc ttgaatcata tcgaccacga aacaaatact tgccgtatca tttatcgggt
    36121 ggaaggtgag gggactagag tcttctctac tctttctgcc ggagaccatt tagatgtcat
    36181 gggtccttta ggcaatggtt ttgatttgtc caatcttcat acaggagacg aagtatttat
    36241 aatcggtgga ggaatcggtg tccctcctct atatgagtta tcaaaacagt tagtcgaaaa
    36301 aggaattaag ccaacgcatt ttttaggttt tgccacacat gaagtcgttt attatgaaaa
    36361 agaatttaat cgtctaggag aaacaagaat cgctactgac gatggaacat acggtatgca
    36421 cggaaatgtc ggtaatttat tgttaggaac gaagacggaa cctacagctg tttttgcatg
    36481 tggttcaaac gggttattga agacagtgga acagctgttt tcttcgcatc aaaatgtcca
    36541 gttatcatta gaatcccgca tggcatgtgg gataggggct tgctatgctt gcgtttgtca
    36601 tacgccggaa gatgaaacag gaacaaaaag tgtgaaagtt tgtgacgaag gaccgatttt
    36661 taaaatagga gaggtaatta tatgaatgag aatcctttag cggtcaaact tcctggccta
    36721 gaattaaaaa atcctatcat gccggcaagc ggctgtttcg gttttggcaa agaatacgga
    36781 aaatactatg atttgaatca gcttggatcg atcatggtca aggcaacgac tccaaatgcc
    36841 cgctttggca atcccacgcc aagagtggcg gaaacaccat ctggcatgtt gaatgcaatt
    36901 ggtttgcaaa atcctggtct agacgttgtt atgcataaac ttcttccaga attagaggag
    36961 tacaatgagt tacccatcat cgcaaatgtt gctggtgctt gtgaagaaga ctatgtagaa
    37021 gtttgtagca aaattggcga agcaccaaat gtgaaagcaa tcgagctgaa catttcttgc
    37081 ccaaatgtca aacatggcgg aattgctttt ggaacagatt ctgatgttgc ttttcaatta
    37141 acacaagcag taaagaaagt ttcttctgtt cctgtctatg tcaaattgtc cccaaatgta
    37201 acagacatcg ttccaatcgc tcaggcaatc gaagctggag gggcagatgg gtttacgatg
    37261 atcaatactc ttcttggtat gcggattgac cttaagacaa gaaaaccgat tttagccaat
    37321 caaacaggtg gactctccgg accagcaatc aagcctgtcg ctattcgttt gattcaccaa
    37381 gtagcaagta tatccagcct tccaatcatt gggatgggcg gcgtgcagac agtggatgat
    37441 gttcttgaga tgtttatggc tggagcaagt gctgtagcta tagggaccgc gaatttcacc
    37501 gatccgtata tctgtcctaa actaattaaa gagctccctg tgcgaatgag cgaactaggc
    37561 atcgaatcgc tggaaagatt aatcaaagaa gtgagagagg aacgtgaacg atgaatcaac
    37621 tagaccaacg accaattatt gcattagatt tttcaactag gcaagaggta gaagactttc
    37681 ttcgattttt cccgaaagaa gaaaaattgt ttgtgaaaat cggaatggaa ttgttctacc
    37741 aagaaggacc tgaaatcgta cgttatctaa aagaggctgg tcataatgta tttttagatc
    37801 tgaaacttca cgatattccg aatacggtcg aaaaagcaat gcggggattg gcaaagcttg
    37861 gtgttgatct cacctgtgtt catgccgctg gcggtattcg tatgatggag gcggcaatgc
    37921 gaggattaga agaggggact ccagaaggtg gaaaacgtcc gctacttcta gctatcactc
    37981 agcttacttc tacaagtgaa gaagaaatgc atgccgatca actaatcgaa gtttctttag
    38041 aaaaaagtgt gatccattat gctagctgtg ccaaaaaagc aggattagat ggtgtggttt
    38101 cttctgcttg ggaagtagaa gcgatcaaag aaatggcagg agacgaattt gtttgcttaa
    38161 ctccgggtat aagaccagaa ggaacagtcg caggagatca gacaagagta gtcacacctt
    38221 ctcaggcaaa aaagatcggc tcaactttta tcgttatagg tcgaccaatc actcagtcaa
    38281 ctaatcctta taaagcatat caaacgattc aaacagaatg gagctaacct aaaatgaatg
    38341 ttgaacaaag tatcgcaaaa gacttattag aaattgaagc agtattttta aatccaagta
    38401 atccttttac atgggcaagt ggaatcaaaa gcccaattta ctgtgataac cgaatcacga
    38461 tgagctatcc aaaagtccga aaagagatcg caaaaggatt agcaagcaaa atcaaagaag
    38521 cttaccctga agtacaagtg attgctggaa ctgcaacagc aggaatccct catgccgcat
    38581 gggtagcgga aatactggat ttgccaatgg tctatattcg aagtaaggcg aaagatcatg
    38641 ggaaaggtaa ccaaatcgaa ggacgtatat ttgaaggtca gaagatggtt gtgatcgagg
    38701 atttgatttc tacaggcgga agtgtcttgg aagcagctga agcagcaaaa agagaaggtg
    38761 cggatatcct tggtgttgcg gctatcttta cgtatgagtt gccaaaagga aaagcaaatt
    38821 tcgaaaaagc cgaaatccct cttatgacgt tgaccaatta ttctgtattg attgaagcag
    38881 ctttagaaga tcgttatatc gatgagcaag aattaactct gctaaaagaa tggaaaaaag
    38941 atcctgaaaa ttggcagatt taagcgcgct tgatgaagat agagaaatcc gatttgtgtc
    39001 tccagaatca tcaattggaa tagcaaaaat ccaaaaactg attgcgagaa gcatcttttc
    39061 atcaggtatg aaaagaaact tctatcgtga tcagtttttg atttttttaa aattcatttt
    39121 tctaagactg gttagcagaa agaagagaat aatctcttct cttttttcta ttatatatag
    39181 attttctcta ttttttggta agggagctgt ttcaaaaatg gtaaagagcg gaaatgttgc
    39241 gaatatcagt tatttaatga tatgatattc ggaaaaagaa atctcaaagg gaggtaactt
    39301 ttatggggtt gaatgaattt attttaggat tgaacaattt agagacgatc acaagggcac
    39361 ctggattttt taaatataca gagcatacag ttgcagcaca ttcgtatcgt gtagcatcta
    39421 ttgcgcaagt tctaggagac attgaagaag cgaatggaca accgatcgat tggaaactgc
    39481 tctatgaaaa atcattgaat catgactaca cagagcgttt tattggtgat atcaaaacgc
    39541 cggtgaaata cgcaagcaaa gagttaagag ggatgctgca aacagttgaa gaaaagatga
    39601 cggacgaatt catcaaccaa gagattccag ctgagttcca agaagtttat cgccgtcggt
    39661 tgttcgaagg aaaagatgaa accgtagaag gagaactatt agccatttca gataaggttg
    39721 accttcttta tgaatctttt gatgaaatca tcaaaaataa cccggaaaaa gtttataagg
    39781 agatgttcat tgaggcagtg actacgatta aaagctttga acatcggcct tctgttcagt
    39841 atttctttaa agagatattt ccggaactat tgaaccagga attttatgga aaagaggcgt
    39901 ttttagaaac cgtctcaacg attgtaaaga aagaagaaaa ataaaaatga tatgagatgc
    39961 agggggaagt atagttgaaa aagaatatgg atgtcgaatg gggctattct ggagacaaag
    40021 gtccggaaaa ttgggcgagt ttatgtgatt ggtttgcgag aggtgcggaa tttccattgc
    40081 agtcgcctat tcatttgacc aagggggaag aaaccaaaat agaaggagac acgctttcct
    40141 ttcattatca aaagcaacgg tttactgaca aggaattcaa aaacacgatt catttagtac
    40201 cttttgacca actcagttat gtagagttca agaaagaacg ctattatttg acagatattc
    40261 atttccactt gccttctgaa catatcatca acggaaaaca ggaagatatt gaatttcatt
    40321 ttgttcatat gaacagcaaa aaagagaatc tagttgtggg tgtcatgtac cagctaatgg
    40381 tacaggaagg ttggctgtat aatcaagaaa atggggaaag ttggaactta gaaaaacatg
    40441 agcattgggt aaatccggat gtgttctttc cggagcaaaa aagccatttc cattatattg
    40501 gctcattgac aactccgcct accagcgggc caatccaatg gtttgtaatg gatcatgtag
    40561 gaaaactgaa ccaagagttt cttctcccct tcgatggtca gtatgctcga ccgaacaacc
    40621 gaccaatcca gcccttgaat ggaagagaaa tttattattt cgaggagttt gagcaatgaa
    40681 tattcagcag atgaaatacg tggtcgctgt agcaaacaat ggtagtttca gagaagcggc
    40741 aaagaaaatg tttgtctccc agccaagtct ttcagctggt atcaaagaat tagaaaccga
    40801 gttagggatc acgctgttta ctcgaacgaa tcgaggggca tatttaacag aagaagggca
    40861 agacttctta aatcgtgccg agaaaatatt ggtccagctt gatacattag aaactcacta
    40921 tctaacgagt gaaaagagag acaatttttc gatcgctgcc cagcattatg atttcttagg
    40981 tcctttgacc gcacagctac tcgaggaatt caaagacgag atcaaacaat ttcggatcat
    41041 ggagacgaca acagcgagag tgatcgaaga agtcaaagag cagcacagtg agatcggcat
    41101 tatttatatg aatgagcata atcaagctgg tatcagaaga tatttacaac aaggagagct
    41161 aagtggccgc agcattggaa aattcaaaac gcatattttt ttaaggcaag gacatccatt
    41221 agctgaacac gcagagatca ccaaagaaga attgttatct tatcctcaag ttcgttttac
    41281 acaggatgga aataatttcc cgtattttta tgaagacctt atcgagatcc ctgatcaaga
    41341 aacagtgatt tatacgagtg atcgaggtac tttgatgaat atcgttcttg aaactgatgc
    41401 ttacgcttca ggatctggta ttgtgattgg agggattcgc gagcacttgc ggttaatccc
    41461 tttggcagaa ggcgaactaa atgaatttta tatcatttat tcggcgaaac gtccgctcag
    41521 tgaaatcgct caaagattta tcaaagagct tactcggtta ctggaccagc agagctaaga
    41581 aaagcagatg agaacgctgt ttattgaaca ccgttgatca aaaaagaggg gttgtgacag
    41641 aattttgtca caactcctct ttttgtagca aacaatttat aactaattgc tgttgtggga
    41701 aagtacgcaa atatgattct agaaacactc attgattacc tgttttgttt catagctgta
    41761 attttttctt cttctggtgt cacgataatc gtcttttggt tttcataaat aacataacca
    41821 ggcttggcgc catttggttt tcggatatgc tttacctgta caagatcaac cggcacttgg
    41881 gcagagtatc ggtattttga aaaataggca gctaactcgg cagcttctgt aattgtttca
    41941 tctgagggct tgtcactttt aatgatgacg tgcgaaccgg gtatattttt tgcatgaagc
    42001 caatagtcgg tttttttagc tgtcttcatt gtcagctggt cattttgaag attgttttta
    42061 ccaacaagaa tgagcgttcc gtcagaagaa aagtattgat caggctgaga tggtttctta
    42121 cgtttttgtt tttttaagtt cttctttttc aggtatcctt ctgaagtcaa ttcttcttta
    42181 attgcttcga tatccattgg gccggcaatt tccagttgag ataagacaga ttcaagatat
    42241 tggatttcat ctttggcttc ttggatttgt tcgccaatca gcttaacagc gttcttcagc
    42301 ttttggtatc gatggaaata tttctgtgca ttttgatttg gtgtcaatgc cggatctagt
    42361 gctattttga ttggccgatc ttcttcataa taattaggta aaacaacttc ttttgcgccg
    42421 cgtggtactt gtgtcagaaa agtagtaagt agttcgccat ttctacggta attctcagcg
    42481 ttttcggatt ctttgagtgt ttgttcacgt tttttcaatt tatttttatt tcgttttagt
    42541 tcattttcga tttttcgaat cagttctccg ccttgttgtt ttgcccgatc tttctctgct
    42601 ttttcgtgat aatagctatc taataattga ctgagtgttg gataagctgt tacagcagat
    42661 gcctgttcgg acaatacctg ataagcaatc ggtgtgaaaa aatctttatt ttccgtttcg
    42721 taaaaagtag gaataggctg atggctgatg gctgagaaaa attcgtccca tacaaccatc
    42781 ttttcattag gacgttccag taatcttttg gtcagctctt gagcgatatc gaaaccgatt
    42841 ccctgaaact gacgttggat tgcttttgga tctagttcag tttgggaaag ccgctgaaag
    42901 attttttctt tctcggagct aaatgggttc aagacctctt gttttggcgg agcaacatat
    42961 tctacaccag gaagcaaaga acgataagtg ttttgagagc ttccaatgtg tttgatcgca
    43021 tccagtattt ttccagtttc tcgattaact aagacgatcg tactatgacg tcccattaat
    43081 tcaacgatca atatgatatt ttgtagatca cccaactcgt ttcgtttagc aaagtggaaa
    43141 tggatcactc gatcattttc gatttgttcg atgtcttcta aaatagcgcc atccaaatat
    43201 tttcgaagca tcatcacgaa gttaggcgga ttatcaggat tctgataatc gatttgggta
    43261 atctgtacac gagcataact tgggtgcgca gataataata atcgatggtt tttgccgcgt
    43321 gaacgaatca ccagcacgat ttcattttca taaggctgat ggattttcga gatccggcca
    43381 gatagtaacg tttcacgcaa ttcgttgacc atcgcgtggg taaatacacc atcaaaggac
    43441 atggttcatt cctcatttct tttcataagt atattcatta taacgaaaaa aagaagaaaa
    43501 accaatttgg ccgaatagta aagcaaaagt ctagaaaaat cttggactaa ttacataaaa
    43561 aacagtgttt ctatcgttga aatccaaatt tttaggt
//
LOCUS       DACEOM010000015        42765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEOM010000015 DACEOM010000000
VERSION     DACEOM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.016"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            77..361
                     /locus_tag="IW781_000799"
     CDS             77..361
                     /locus_tag="IW781_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1181815.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(642..1061)
                     /locus_tag="IW781_000800"
     CDS             complement(642..1061)
                     /locus_tag="IW781_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1181816.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1063..1647)
                     /locus_tag="IW781_000801"
     CDS             complement(1063..1647)
                     /locus_tag="IW781_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1181817.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1694..3169)
                     /locus_tag="IW781_000802"
     CDS             complement(1694..3169)
                     /locus_tag="IW781_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1181818.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3350..3661
                     /locus_tag="IW781_000803"
     CDS             3350..3661
                     /locus_tag="IW781_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181819.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3776..6112)
                     /locus_tag="IW781_000804"
     CDS             complement(3776..6112)
                     /locus_tag="IW781_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1181820.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6254..6922
                     /locus_tag="IW781_000805"
     CDS             6254..6922
                     /locus_tag="IW781_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1181821.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6925..7968
                     /locus_tag="IW781_000806"
     CDS             6925..7968
                     /locus_tag="IW781_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1181822.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8205..9086)
                     /locus_tag="IW781_000807"
     CDS             complement(8205..9086)
                     /locus_tag="IW781_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1181823.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9245..9973
                     /locus_tag="IW781_000808"
     CDS             9245..9973
                     /locus_tag="IW781_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1181824.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10129..10446
                     /locus_tag="IW781_000809"
     CDS             10129..10446
                     /locus_tag="IW781_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1181825.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10439..11047
                     /locus_tag="IW781_000810"
     CDS             10439..11047
                     /locus_tag="IW781_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1181826.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11063..11893
                     /locus_tag="IW781_000811"
     CDS             11063..11893
                     /locus_tag="IW781_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1181827.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12031..12519
                     /locus_tag="IW781_000812"
     CDS             12031..12519
                     /locus_tag="IW781_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1181828.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12570..13442
                     /locus_tag="IW781_000813"
     CDS             12570..13442
                     /locus_tag="IW781_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1181829.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13618..13980
                     /locus_tag="IW781_000814"
     CDS             13618..13980
                     /locus_tag="IW781_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1181830.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13989..14741
                     /locus_tag="IW781_000815"
     CDS             13989..14741
                     /locus_tag="IW781_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181831.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14755..16557
                     /locus_tag="IW781_000816"
     CDS             14755..16557
                     /locus_tag="IW781_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181832.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16834..17874
                     /locus_tag="IW781_000817"
     CDS             16834..17874
                     /locus_tag="IW781_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1181833.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17926..20070)
                     /locus_tag="IW781_000818"
     CDS             complement(17926..20070)
                     /locus_tag="IW781_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1181834.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20427..21800)
                     /locus_tag="IW781_000819"
     CDS             complement(20427..21800)
                     /locus_tag="IW781_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1181835.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21800..22195)
                     /gene="rbsD"
                     /locus_tag="IW781_000820"
     CDS             complement(21800..22195)
                     /gene="rbsD"
                     /locus_tag="IW781_000820"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1181836.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22223..23140)
                     /gene="rbsK"
                     /locus_tag="IW781_000821"
     CDS             complement(22223..23140)
                     /gene="rbsK"
                     /locus_tag="IW781_000821"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1181837.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23248..24261)
                     /locus_tag="IW781_000822"
     CDS             complement(23248..24261)
                     /locus_tag="IW781_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1181838.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24342..24941)
                     /locus_tag="IW781_000823"
     CDS             complement(24342..24941)
                     /locus_tag="IW781_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1181839.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25127..25828
                     /locus_tag="IW781_000824"
     CDS             25127..25828
                     /locus_tag="IW781_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181840.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25838..28549
                     /locus_tag="IW781_000825"
     CDS             25838..28549
                     /locus_tag="IW781_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1181841.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29000..30628
                     /gene="treC"
                     /locus_tag="IW781_000826"
     CDS             29000..30628
                     /gene="treC"
                     /locus_tag="IW781_000826"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1181842.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30688..31869)
                     /locus_tag="IW781_000827"
     CDS             complement(30688..31869)
                     /locus_tag="IW781_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1181843.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32121..33644
                     /locus_tag="IW781_000828"
     CDS             32121..33644
                     /locus_tag="IW781_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1181844.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33713..34441)
                     /locus_tag="IW781_000829"
     CDS             complement(33713..34441)
                     /locus_tag="IW781_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1181845.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34454..35734)
                     /locus_tag="IW781_000830"
     CDS             complement(34454..35734)
                     /locus_tag="IW781_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1181846.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(35875..36477)
                     /locus_tag="IW781_000831"
     CDS             complement(35875..36477)
                     /locus_tag="IW781_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1181847.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36722..36928
                     /locus_tag="IW781_000832"
     CDS             36722..36928
                     /locus_tag="IW781_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181848.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37240..37959
                     /locus_tag="IW781_000833"
     CDS             37240..37959
                     /locus_tag="IW781_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1181849.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38040..38723)
                     /locus_tag="IW781_000834"
     CDS             complement(38040..38723)
                     /locus_tag="IW781_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1181850.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            38941..41127
                     /locus_tag="IW781_000835"
     CDS             38941..41127
                     /locus_tag="IW781_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1181851.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41473..41883
                     /locus_tag="IW781_000836"
     CDS             41473..41883
                     /locus_tag="IW781_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1181852.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            41897..42253
                     /locus_tag="IW781_000837"
     CDS             41897..42253
                     /locus_tag="IW781_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1181853.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        13940 a         7524 c         8542 g        12759 t
ORIGIN      
        1 ctgcgacaag acttttgtca cagttccttc ttttttttgc tatgctatac tagatgtaaa
       61 aaataggaga aataaaatga gggaaattta ttcttttgaa tcaaaaatct atgcttccga
      121 agtcggcaag ggcggggcat atattatttt tccgtatgat ataagaaaag aattcggtgc
      181 tggaagagtt aaagtccaag caacttttga caatgtgccg tatgaaggca gtatcgtgaa
      241 tatgggcgtg aaaaatccag atggttcagt ctgctacatc cttggactac gaaaagatat
      301 tcgtaaaaac atcggaaaag aaatcggtga tattgtagca gttacagtga agcaaaaata
      361 aaagaggttg tgacaaaaga cgtgtcacaa cctcttgata aacggtgttc aaaagccagt
      421 ttctcggtag taagtcaaat ttatgcaaaa atgtggaaac ccatgtttac aaaatttttc
      481 ttactactcg aaacgaagcg gctttatcac aacctcttga taaacggcgt tcaatcagct
      541 gtccctcggt attagctcaa aaacggcaaa atatgagaag ctatttttcc atttttttct
      601 ctcatactca ggtcataacg ctgatttcac aacctcttct ttcataatgg tttttcacca
      661 tctttataat aaactggttc tttgatatgg aaatctattt tttccaacca atctaaaatc
      721 ttgtaaccta actcttctga catcggaaaa gaggcgttga tttgtgcatt ggagatatgg
      781 tcaacatgga taccggaagt gatcgtacaa cttcccggaa cggcagtttt gatgatcaca
      841 gctatttttt cggctaagac atcgtcttta tggaaccgac catcgtggct agggaaacgt
      901 acagtttgca tctctgtatc acgagaaaat gtagtgactg ttccgatatg tggtgtatct
      961 ccgcctgtta aagtgatcaa taagtcttta ccgattactt taacagcagc ttggatcgtg
     1021 aaacctagtt tggttacttc aactatcgtt tctttttcca tattaatcac cctgccaacg
     1081 ttttgaatgc tcatttacaa tttcaaattg atccaaaagt ttcattgtat ggtgattaac
     1141 aagatcttgg atcgtttgag gctgattata aaaagcagga attggtggta tgatttgcac
     1201 acctagtcgt gctaatttag tcaagttttc caagtgaatg gcatttaatg gcgtttcacg
     1261 aggaacaatg actaactttc gttgctcctt cattgttaca tcagcagcac gtgcaatcag
     1321 gttgtcgcct aaaccaacag agatacctgc gactgttttc atacttgccg gtacgatgat
     1381 cattccatcg gtcaaaaatg atccgctggc gacagcagca cctaaatctt tgtttgaata
     1441 atggtaatca aacaggcttt cgaattcagt tagtgataag tcggattcca gctttaagtt
     1501 ctgttttgcc catggactga tgattccgtg tgtttctatc atcggatatt cttttagttt
     1561 cttcactagg ttgatggcat aaatggtgcc tgaagcgcca cttacaccaa tcacgatttt
     1621 tttcttccgt tgtgccaatt gttccatttt ccgcactcct ttttatgatg tcaaaacagt
     1681 tgcagatcat tcattatttc aaatattttt cccaatcttc tacttcttgg aattttgcac
     1741 gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc aaagatagtt tttgcactta
     1801 tcccacggaa acgaatagag ccaggattat attctggtaa ttctgatggg tccaatggat
     1861 ggttcctcat tcctgggatc acgatgatat ctttatctcc ttggaaacga gtgttgaaag
     1921 tccaaactac atcgttcatg tcgaaaatat caacatcttc atcgactaaa atgactgtct
     1981 tcaattcttt gaaggctgat aaagcaagaa tagctgcttg acgctgaata ccttcatccg
     2041 cttcgttttc tttacggatt tgcatgatgg tcatcaattt accaccacct gcaggaggat
     2101 tgtagacatt tactactttt cctggaatag ctttatcgac caaggcaaga atacttgctt
     2161 ctgtaggaat accggccata gatacgtgtt cttcactggg accaatcgtt gtttgcatga
     2221 ttggattgtc ttttcggtga gtaactgctt tgactttcac tacttgtaca gctggattag
     2281 catccccagt atagccagga aattctggca ttgcttttcc tgagttcgtg ttgatatctt
     2341 cttgcattgt ttcatttggc atgatttctg cttcaatgat gaattcagca cgagcaatac
     2401 ccacttcatc gactgctata ccatcaacga tttgaatagg ttcgttacgc aaagctcctg
     2461 cacaccatag ttcattgtag ccaagtggag tggtaggtgg ttcgaaagtc gtgccaatgg
     2521 caattgctgg atctaaacca atattgatag tgataggcat tggtttattc aaggcttcga
     2581 attgtttttg gaagtgacca atgtggcgtc cgccaggcat gatgtacata ccgatcgtat
     2641 ctttatcttc tagaaccata cgatggatcg ttacatcgct cattgttttt tctggatctg
     2701 atcctaagac tacacccatt gtgatgtatg gaccagcatc ttcaggagta tttgtaggtg
     2761 caggcaaaat gttgcgaata tcgaagtctg catcggtagc tagatgaacg acttcttgtg
     2821 ctggagcgtc tgctttgtct actttcacag gtttgattgg attttctaca gcgtctttca
     2881 agaaacgacc aagtgttcga taatcatggt gcaaaatttc acctacacgt ttacgactag
     2941 ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt gatattgttg aaagtcatag
     3001 ctggtccttc ttgagtcgga cgttttactg taccgccagc accgatataa cggtaaacac
     3061 cagataattc tgcttcagga tcgatttcaa tatctgtttc gtgatattga cctggaagtg
     3121 tttttagttc ttcgatgact tttcttaaat catacggttg atctgtcatg agagattcct
     3181 ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa gaggaaaaca attcaacttg
     3241 ttcggtaaag tattctacaa tggaatggat gcgttttttt gataatagaa taaactatga
     3301 tatacttatc actaaaagaa agcgcatact tagtgttgga ggaaggaaag tgaaaaaaat
     3361 ggaggagtca tttcctaaag cagtaaaagt ggaaaatata gcgaatattt tgaaagttac
     3421 atttgaaaac ggagaagtaa aatacgtgaa atctcattgg acagaagaga taaccgatgc
     3481 cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat cttctcgcac tttctcgtaa
     3541 tatgtggatt gggactgaag taacgattga agcagatgga acagtattca taaatggaaa
     3601 agacaggtat acgccagaag agttgtggta caaaggaaag aaaagtatac cagaattata
     3661 aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat tacttcctcg tttcttgctg
     3721 ttttctacta tttcagcgct cttatgactc aaatctaaat gtggttgtca caaccttatt
     3781 ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat tcgtgcagga agatgaccgc
     3841 caagcatcgt caatatagta gagacgatca ctagaataat cgcatggaca ggatttagtt
     3901 gagcaacatt ttttagatcc gtcatgttgt ataagatgct attgataggg aacgtagcta
     3961 gccatgcaat aaacacacct aatatcccag aagaaatacc taaaatacac gtttctgcgt
     4021 caaatacgcg agtgatgtct tttttacgtg cacctaaagc ttttagtaca ccgatttctt
     4081 ttgtccgttc aatcacagat gtataagtaa tgatactgat catgatcata cttgttacta
     4141 aagagatccc tgcaaaagca ataaggacat aagtgatagc atccatcaat ccgccagtca
     4201 gttcagtcat cgttccagct aaatcagtat aaatgatttt atcttcatct gatttgcctt
     4261 tattgtaatc atctagataa tccaagatct tttctttgtc ttcaaaattg ttcggataaa
     4321 tcatgatact tgaaggcaag ctgtctccac ctaaataact aagcaatgtt tgtttggcat
     4381 tttcgtcaac tttttcagta gttaagacat ttacaccact gtctctttga gctttgacga
     4441 tttctgattc tttgttttcg ttgacgattt gagtcgttaa ttgatcgcta taagcgattc
     4501 ctggagataa gagattcatt gtagaagaag atttcacgcg taggatcccc gagatcttca
     4561 attcatcaga agcattctga tacatcgcat cataatctgt attagggata aaattcccag
     4621 ttggaagttt tgtatagtaa gtattgttgt ttactaattt gaatgttgta ccaacgatat
     4681 catcaaaatc aagtttttcg tcttctttca catcaaatcc aagattcttc aatgcgttga
     4741 tattagtatt attattgccg tctacgatca gcacgacatc tgttgctgag gctggataac
     4801 ttccggcaag taaagaataa ttgtctttta aaaagttttc tttgctagtg tcaagctgtg
     4861 tagggaaact tgacactccc acacctgtca ttgctgacat cgtactagat aaagaaagag
     4921 attccgtatc aggattttga ttcgagaagc tgacaggctg tactttgcca tttacatcac
     4981 gtaaaagatt gattcctgta gtacgggtaa atccgatatt attacttaag ttaggatcga
     5041 tgtctgttac gtaatcgacg tatgtttgat caatcttatt cgtgtgctgt gctttatctt
     5101 cttcgctgac ttttgcagta atcgttttgc tgtctggata atccgctttg cttgcaccca
     5161 atccggcatc gtcacgagtc tggttagtcg ttaccttaga aatagtaatc ggaaatttcg
     5221 ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa tccagaagaa agggacagaa
     5281 cgatcgcgat tccgatgatc ccaatactgg aagcaaagga ggtcaagaaa gtacgtcctt
     5341 tttttgttcg gatattgttg aaggaaagct ttaacgctgt ccaaaaactc atctttgttc
     5401 tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg aggatgggaa tctgttaaga
     5461 tcttaccatc agaaaattca atgattcgat ctgcgtattg atgggccagt tcaggattat
     5521 gtgtgaccat gatcactaat ttttctttcg ataactcttg gatcaattcc atgatctgga
     5581 tacttgtttc tgtatctaac gctccggttg gttcatcgca caaaaggata tctgggtcat
     5641 tggctagtgc tcgagcgatg gccactcgct gcatttgtcc accagataac tgatttggct
     5701 ttttatgcat atgatttgtc aagccgactc ggtgcaatgc atcttctgcc cgttttcgtt
     5761 tttcgtcttt agaaacacca cttaatgtca tcccaagttc aacgttttca ataatgccta
     5821 gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat agagttgttt cggtaggcat
     5881 cccagtcact gtccttaaag tctttagtag actttccgtt gattaccata tcaccggaat
     5941 cataattatc tagtccacca atcacattca gcatagtcgt tttaccagaa ccactaggac
     6001 ctaaaatcgc tacgaattct ttttgtctga aagcgacaga cacaccatcc aatgctttgg
     6061 tggtggtttc gccgacttta taatattttt ttatatcttt taattgaagc ataatcaccc
     6121 ttctcccgta ttgaaatatt cggtatacta ataagtatat cagaacaatg atcgtagtat
     6181 acgaaataaa tgttaactga agatgaacta caagaaaaaa tctagaaatt tttcaaaaaa
     6241 cagagggaga tcaatgaatc gtattttagt agtagaagat gatccgcaat tacagttgct
     6301 ttaccgttct gtgttggaac gagcgggctt ttcagtcatc accgcattga atggagagga
     6361 tgcgctgaaa aaactagaag cgacacgtat agaattaatc attacagata tcatgatgcc
     6421 gaaaatggat ggctatgact tgctagactc attacgtagc accagatttg acacacctgt
     6481 tctaatgatt acggcaaagg ccgattttga ggataagaaa cgcgggttca aattaggagc
     6541 agatgattat atgacaaaac ccatcgatgt caatgagatg atcctacgtg tggaagcatt
     6601 gcttcgacgt tcaaagatca attacagcag tagcttgata atcggacaaa caaaattaga
     6661 gctagatgct tatacagtgt tacaagatca gcattcgttg attctgcctc aaaaagaatt
     6721 tctgcttctg tataaattac tgtcttatcc caacaagatc ttcactcgcc aacagttgat
     6781 ggatgatatt tggggattag atacgaatac agaagaacga acgatcgatg tacatatcaa
     6841 acgtttaagg acccgttttg aaaagacgga agattttcaa atcatcacag tacggggact
     6901 aggctataag gcggttattt aaagatgaaa aaaaagctat tttcacaatt atggatctat
     6961 tttgctagta ttgtcttcat ttcgatctta gtgacactgc tgtgttttct cggttttatt
     7021 ttcttattgt ctcgacattc tatctcgcct gctgaatcgg ctggaaaacc aacgttatac
     7081 ccgttattcg tctttgcggg gttcagtatg gtcgtgggta caggaatatc gatttttgtt
     7141 gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa atatgagtct ggtagcaacg
     7201 ggagattttt cgattcgaat ggatgaacag cagaaagtag cagaagtaca gcaactgtat
     7261 aaagatttca acgttatggt ccaagaactc aatagtatcg agacactgag gaatgacttt
     7321 gtgtccagtg tatcccatga attcaaaaca ccactagcaa caatccaagg atatgttcaa
     7381 ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa tatttttata tcggattatc
     7441 gaaagtatca cgcagctttc acaattaaca gagaacatct taaaattgaa caaactggaa
     7501 aaccagcgta tccaactgga aaagaaagag tatcgattag atgaacaaat cagagaagta
     7561 atcgtttttt ttcagcctaa atgggagaaa gaacagctag aattggatat cgaacttgct
     7621 gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag tgtggctgaa tatcatggat
     7681 aacgccataa agtacaatca aatcaatgga cagattcata tcaaactatt tgagacagcc
     7741 acagagattg tactggaagt gaccgattca ggtgtaggaa tgaatgaaga gaccagagat
     7801 cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc aaatttctgg gaatgggcta
     7861 ggactttcac tggttaaaaa aattctagaa ctgcatgatg gacgtattga ttatagcagt
     7921 atcgaaggag taggaacgac agcaatgata cggttaagta aacaatagga aaaagaataa
     7981 aacccctaaa aattctattg aaggttttta ggggttttta gtcgtttttt tgtgattagg
     8041 gaaatgttct tacggttctt ttggcataaa aatcgctgct ttcttattga actacgagaa
     8101 atgttagagg tgtcattgaa acaaggacat gatgcaagaa agagagcgaa agtcatgcgt
     8161 cgctctcttt ttttgggtgg atagatggac agaaaatgta gggcttagtt ttcttcttga
     8221 tttaaatact cccaaaatgt atctgaagcc aaagaatgct caccgacttt acgagtaaat
     8281 gcaagataac tctctgttgt tggagaaata gggagaatca cagtattttc ttttaataga
     8341 ttcttgactg ttttttccat aacaatagca atccccatgt catttgcaat catattgatg
     8401 atcaagtcga ttcttgctcc tttataagag atttttggtt caaatccagc ttcgcgacac
     8461 agatctagta caggttgata caagttcgta gtggggccaa ggagcaaaaa gttctccttt
     8521 ttcagttcgc ttagatccaa tttcttttta ctagctaaag gatgattttt aggtagaact
     8581 gctacaaaat cgtctgtttc agtaggcaac cactcaatcg aacttggtgg ggcctggaaa
     8641 cttctagcaa agtggatcgt gtcactatgt tcattggcgt cattgtaagt taattcgaca
     8701 ctttccactt ctttcaactg gactgtaaat tcaggatggc gtttcaaaaa ctcggtgatt
     8761 ttggaaaaac ctttataatt gatcattgtc ggtatcgtta agatatttaa tgaaagattg
     8821 ttgtctttcg tataggtttc aagcacttct tgcatagcat ggtattgttc tacgacattt
     8881 tttacatatg ggagcaataa gcttccagct tcggtcaatt cgatttttct atgggcgcgt
     8941 tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa tctgtttaga aatatttcct
     9001 tgggtggtga attgtcgatc agcagttttg gtgaaattca ttgtttcggc taaatcaaga
     9061 aaaacttcga atttatggat atccatcata ttcctcctac ttattccaat aaagaatact
     9121 ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt tacaatcttt cttgcagcga
     9181 aagcaataat tacagaaatt tttctgattt ttttcgcttc gtagaaaaag agaggagttt
     9241 tttgatgcca actggagttt tgatcaatgt aggttccgtt ttgtttggcg gactaattgg
     9301 cggattagta ggaaataaat taagtgaaca ttttaaagca cagctgacga tggtttttgg
     9361 tgtgtgttcg atggggatgg gaatctattc cattgcgccg atgaaaaata tgccggctgt
     9421 tatttttgct ttagtgatag gaacagcgat tggtttgatt gttcatttag gtaatgggat
     9481 aaataagggg gctgcgctga tgcaagtacc tatttctaaa atcttcccta gtgaaaagct
     9541 tggtatgacc cacgatgaat ttatcagcac attagtgacg gtcattgttc tattttgtgc
     9601 aagtggtacg gggatctatg gaagtttaga ttctgggatg acaggagaca gtacgatttt
     9661 gatttctaaa tctgtccttg attttttcac agcagctatt tttgcgtgta atttagggta
     9721 tgtcgtttct gtgatagcaa ttccccaatt cattattttt tatattttgt tcttattggc
     9781 aaaatttatt tatccattga ctacaccaga catgatctta gattttaaag cttgcggcgg
     9841 attcttgatg gtcgctaccg gttttagaat gatcaacgtg aaaatgttcc cagtagctga
     9901 catgatacct gcaatgatcg ttatcatgcc actgagctgg atgtggacaa attggattat
     9961 gccactgtta taataaaaat caaagagaag ccattttttg ctgactaaaa aggatcaaat
    10021 cttttagtca gtttttttgt atttgacaaa ctgattttga aactatatta tatgttgtat
    10081 aatatagatt gaaataaaat attacacttg agaatagaag gtgacgtaat gcacatccag
    10141 attccaactg ttcttcttga tggtactgtg ctagcagcat taagcaagga agatatgtat
    10201 gggtatgcct taacaaaaat cgtacaagaa agtctctctg tcagtgaatc tacactatat
    10261 ccggttttga gaagattgaa aaaagaagga tatcttgaga cctatgatga accttttgaa
    10321 ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa gtcgtcattt agaagaaatt
    10381 attgatgaat ggattgtttt tcgagattct ttagaccggt tattggagga gagcacaaat
    10441 gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa attcctgaag acgatcgttt
    10501 tgatttgatc cagtattatg aggaatattt tttagattct ggaaaaacat tagatcagat
    10561 cattgatgaa tatggtacac caaagcagtt tgccttaaaa ttaaaaatca gttacttttc
    10621 agaagaagaa caagcagaaa acgagttagt gaaaaatcca aaaagacaaa ttcgcctgat
    10681 ttggttaatc attatcggtt tatttgcttc tcctgtcctc attccactag cactggggtt
    10741 tatattggtt attgggggac tattggttgc tttactagcg ctcattttta gtatctatgt
    10801 tttatgtatc agtatcttag gtgttggcct attcatgatc atctcaggaa tcagtgtgtt
    10861 tggacaatcg attctgagtg gattattctt tatcggtgtc ggtctgttcg ccacaggaag
    10921 cgcagtattt atttcgccac tgttgctgaa aggaacaaaa gggctttttt atgtcatgat
    10981 gaactttgtt aaatggatgg gacgacgttt tattacaaaa agagatctgc attcagcggg
    11041 cgtttgaagg aggacacata aattgagatt gaaacatttt ttgattaccg gtattggtct
    11101 gatgcttact ggtggcctcc tagcaggtgc aacttataca ttaggcgctc aaaagagtct
    11161 gacttgggat aatggtcctc ggttgatcga tatggttact gagacaaaaa aaatcaagga
    11221 tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt tcggtcatac gcggattgga
    11281 ttttgagata aaaacgactt atgataagac agaaaagcca gaaataaagg agaaaaatgg
    11341 cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca atcattagtt tagaagacac
    11401 aagtgcacaa atcactattg ttgtccctgc agatcatgaa ttatctgaat tgacaatcga
    11461 agggaataat agtgcaattg atatcaacga agtatcaatc aaaaaagtga atactaagac
    11521 acatgatggc tgggtatcgt tcaatgaatt tacaggagga acaataactg cgaaagatga
    11581 attaggctca cttcgattag atgaggccaa tgtggataac ttgatcgtca caggtgaaaa
    11641 cagcaacttc catctagaag acagtttgat cagcaaagaa ggaaaagtca tcctaaacgg
    11701 tggcaattta tctttgcatg agatggaaaa cagtggctat actatccaaa cgtctggtga
    11761 atcttatatt gaaaaagact accagccaat aacaaatact ttgttagaac aaggacagaa
    11821 aaagctaaag atcactgcta aagacacatc gatcaatatt acgacggatc aagaggacaa
    11881 tgaaacagat tgatcctgta ctcaactgga tattttaaga aaaaacagca aagatccttc
    11941 caatctttgc tgtttttcat cgcttaacgc acataactat agtaaaataa gaaagaaaat
    12001 atctaaaaag aaaacagggt gaaagagtat atgaaggtag aagaaggtca aagagaaaag
    12061 ttaaaaacag aaatggaaag attacatact tatatcactc aactaagcca aaccttctat
    12121 gatccagaca aagaaaaagt gatggttaat tacccgaaca attcagaagg tcgccaatta
    12181 gagcaagtgt accatgaagt tttcaaacat ttattgacgg tgaagaaaga gctggattat
    12241 tattctttac cgattatcga tacaggaata ttgaagtatg atgggaagaa ggaacgattt
    12301 atctttaaat ctgttcggga agatttgcct ctttcagcag ggatggatct agaggtcttg
    12361 gtagaagact attttacaga agaaaagcac tgggttcgaa caaaattgga ttatttaccg
    12421 caggcagccg gagggacaca ggcaagcggt tggtacatca ctgaagacaa agagttagaa
    12481 ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa aaagtaacat tagatattta
    12541 ttgaaggata aaggagcgca ggaagtagca tgagagaaaa aagagcattt tatatcaatg
    12601 gttatgcagg gattgtcgga ttggtcgtat tagctttagt tggattgttt ttcttctatt
    12661 taggtatgtg gcaagcgaaa gtttgggcac tttttttaag tatttttctt tggttgatca
    12721 cgttattgtt gttaagttcg gcaactgtag tcagtcctaa tcaagcaaaa gtaattttgt
    12781 tttttggaca atatttaggt acgattcgag aaaatggctt tttccttacg atacctttgg
    12841 cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa tagttctgtt ttaaaagtca
    12901 atgatttgga tggaaacccc atcgaaatct cagcagtagt agtctttaaa gtcgttgata
    12961 cagcaaaggc attattcgat gtagcgtatt atcaagattt tgtagaaatc caaagtgaga
    13021 cagccattcg gcatatcgct agccaatacc cgtatgatac attcaatgag aatgatttga
    13081 cgcttcgagg aaacacgacg gcagtgtctg acgagttgca aaaagagctg caagagcgtt
    13141 tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa ccatttagcc tatgcaacag
    13201 aaatcgctag tgccatgctc caacgtcaac aagcaaaagc cattttgtct gctcgacaaa
    13261 ccattgttga aggagctgtc acaatcactc aaatggcctt agagcaaatc gaagatggac
    13321 aagaaatcaa tttcaccgat gatcggaaag tccaattgat caataattta cttgtctcaa
    13381 tcattacaga caaaggaacg caacctgtta tcaatacagg cgacatgaaa gaccgtaagt
    13441 gacatacttt ttgtaagata ggaagaagga gtttaggacg gattattgtc ttgaactctt
    13501 tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag aatgatagtt gattcgaagg
    13561 cagaattgtt cgcttttttc tataatcagt atataaagta agagagaagg ggaaaaagtg
    13621 aaaaaacttc tgatcggtct acttgttttc atcgttgtgg tacttgcagg ggtgaaaatc
    13681 gcagattacg tagttatggg tggagaagaa tactatgtac aaattacgac agatggtaca
    13741 aaaacagttt caaaatcaga ctctggacaa gaaacagtca actatagata tatcctgcct
    13801 ggctatacga aagagggtaa ggaaaaagaa ctggacttca agggacaatt gactcgaccg
    13861 cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa ataaaggtgt cacttcgtat
    13921 gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg aaaaactaat taaagggtag
    13981 ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac aaaagtatac ggaaaaaaca
    14041 atgagaaaaa gacgcaagca ttaagcggca tcagcttcga tgtagaaaaa ggcgaattta
    14101 ttgggatcat gggcgcttct ggttctggta agtcgacact tttgaatatt ttatccacat
    14161 tagataaacc aacagatggg catatccgaa tcaatcaaaa agatgtgacc acattaaaag
    14221 gaaatcaact agcagatttt cgtgcgaatg agattggctt tattttccaa gatttcaatt
    14281 tattagaaag cttgacagca caagaaaata tcgctgttcc tctttcttta caaggcgtca
    14341 gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga acgtttatcc atctctcata
    14401 ttttggaaag ctatccttct gagatttccg gtggacaaaa acaacgggtc gccgccgcac
    14461 gtgcattgat cacacaacca acgattctat taggggatga gcctacaggt gcgctggatt
    14521 ccaaaagtgc ccgggatctt ttagatacga tggacgaatt gaatacaaaa gatcatgtat
    14581 cgattcttct tgtgacgcat gatccgtttt ctgcaagtta ttgccaacgt attttattca
    14641 tcaaggatgg cggtatccat caagaagtga aacgtggaga tcaaagccgt gattccttct
    14701 atcgagaaat actatctatt cttggaaact tggaacagta gggggagatt gactatgctt
    14761 tggaaattat cacttactgg gatcaagagc cgtttaagag actacttggt gttattctca
    14821 ggattagtta tggcatctgc tattttttac atgtttgaat caatggcaag caacgaagct
    14881 tttttgaaaa gtaatacgat cgttgcggct acagttatca ttttccgatt cggttctgtt
    14941 ttactaggga tcatcacatt cgtttatatt ttatatgcaa attccttttt gatgacgatg
    15001 cggcaaaaag actatgcaat gtttatgatg ctgggagcaa aaggcagaaa aatcgcacag
    15061 atgatttttt ctgaaacatt cttagtaggg atcgcagcta ctttagtggg ttcagctgtc
    15121 ggtgtaggat tgacaagtat cgtccaccga ttattagtgg atcagttaga tctcaatatt
    15181 tcgcatttta caccttttag tattcacggg ttgttgatca ctgttttgtt ttttgctgtt
    15241 ttgtttttat tagctgctgt tgtcaatgct ttttcgattg tcaaaaaacc gattttagca
    15301 ttgattcgtg cggatcagac accaacaaga atcaagcagc ataaattctt gtttttcctt
    15361 gaagtagtac tgggaattgc ttttctaggc tgcggttact atatgatgaa acacgtgggg
    15421 acctatcaag tctttggtat aggggtagcc ttagttacga tcgtattagg aacttatttc
    15481 attttccatt ctgtgatcat tttcttttta tcattactaa agaaatcaga acagatttca
    15541 ttgaaaaaat tgaacaattt cacattgtca cagttaaact ttcgtattcg tgaatataca
    15601 caaatgctct caatggtcgc tatgctgttt gccttggcac tgggcgcttt gacggtagga
    15661 ttaggtttta aaaatgaaat tgcagacttg acagaaaaca attctggata tgatctagtg
    15721 atgaataatc ctcgggcaga agatcagcaa aaaatcaaag aactttcacc aacactttct
    15781 gcttcttata ctcaaaaaga agatgcggga acgatttact ataatgcaag tgaatttgcg
    15841 gctcaaccat tgatcatgat aaaacatgaa gatggaacac cgccgaagat cacgaaagaa
    15901 aaagttcctg ctaaaaaaat gaatgaactg actgtacaag aggaacttcg aagctatgaa
    15961 ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc aatcagaatt tgatcaactg
    16021 aacttgccac aatccacttt acagctagtt caggtaaaag acttccaagc aaacctgaaa
    16081 ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc catctttgca aaaagtagag
    16141 tacagcaacc agaaatatga aatgtatgaa gtgtataatg gtctattctc tggttttgaa
    16201 tttatgggat tcttcctagg tttagctttc ttgaccatgc ttgctagctg tttgatgttc
    16261 aagatcttgt caggaagcaa gagtgacatt gctcgttatg aaatgcttga aaagatagga
    16321 acacgacgcg ggttgcttaa acaatcgatc cgtagagaag taggcgtgtt attcttagca
    16381 ccaggaatct taggtgtagt ccatgtctta ttcggattac aaatgtttgg tctattcatg
    16441 caaaatccat acaaagatat ttgggttccg tttacgatat tcttcgtatt gtatttcatc
    16501 tactatgtac tgacgacatg gttatatact ggaatcgtat tgaaaaagag attgtgaaaa
    16561 agctgtcctg catcaagtaa taagaacagc caagcaaaaa tagctcctca tatttttcgt
    16621 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg gaaaaagagg
    16681 ttgtgacaat atttttgtca caaccttttt ttcacggtct tttattagaa tatagtaaat
    16741 tttttctcgt tactcacgga ataagctgtt aagaaaacgg tttccatgta tactggaatc
    16801 agtgcaaatg aaagaaaaga gggtgcaagg aacatggaaa tcaatgaaca aaattttggc
    16861 gaaaactatc acttgattac gatcacgaat caaaatggga catctttgtc tgtcagtgat
    16921 cttggggcac gggttgttca ttttcaaaca ataatcgata atgaaaaacg agagttgatc
    16981 ttaggttttg atgaagcaaa agaatatcta gaaaaagacc cttatatcgg tgcttcaatc
    17041 ggacgtatgg ctggacggat tacgaatggc acattcaaat tagatgatga aacgttcgaa
    17101 gtaaaaacag acccggacac tggtcattgt ttacatggtg gtgcacctag ctttgaagcc
    17161 aagcagtggc attatacagt catcaatggt gaaaacgaag catcggtcat tttctatcag
    17221 actagcccag atatggaaaa cggatttcct ggaactttag atgtagaagt acggtatacg
    17281 ctgacgaacg atgatacatg gcgagtgacg gtccaaggga tcagtgaccg caaaacagta
    17341 ttcaatccta caaatcatgt ctatttcaat ttaaccggtg atccagcaca gtcaatcgac
    17401 gaacatatac tttggttgaa tagccgatta tttgctacgc tcaataaaga tacaacgcct
    17461 acaggagaag tagtttcagt aagtggcaca gcatttgact tccaacgtcc taaaaaatta
    17521 ggtgaggtct ttgccagtga ttttcgccaa tcaatgatag tgggcggcat cgatcatcca
    17581 tttttgttga aaaaacaatc tggtttacta aatgctgcgg aattgacaag cccagatgaa
    17641 aaagtatcga tccaagtaaa aacagatgct tcaggaatcg tgatttttac agcaaatttt
    17701 ggagaagatg gaccggaaat gcgcgggaat aaattagtaa atcatggcgg tatcacattt
    17761 gagactcaag aactgccagg agcggagcga cttgaaagtt ttggagatgt cactctggaa
    17821 cctaaccaag tacgcgagtt tgtaacggaa tacaaaataa atataaagaa atagaataaa
    17881 ttaaaaaggg agtgtgacag tgtcacatcc cctttttaat ttattctatt tctttaaagg
    17941 cagatcaagc cagaatgatt tgtctaaact taacaaggtt agtacaggaa cagttgcagc
    18001 tttaactgta cgagtagttg tttctggtat ctgtcttgcc gtaaaattgt tccacaaatc
    18061 ccagattgaa gcgacacgga agatggaaga agcgttgttg acgcgtttta tcgttgaaaa
    18121 ttctaatcct gtgattggca cgccatctgt tgtccatttg attggcacat cgaatcgctt
    18181 agacatattg aggatttcag cgtttgcttc ttcagttgtt gctgcttctt ttttcggttc
    18241 atcatatttt gtcaggtcgt acgcttcgat gatcgcgttc aatttttgtg aatattggcg
    18301 atctgtagca taacgtcctt ccaaatattt ggttgcttct ttgtagtctt ttgtttccga
    18361 cttccaagtt ggtttgtaga aatcaggatt gccagtcagt ccatttttga tcaagtcggc
    18421 atagtcttcc aaactttcct tgtaagaagg atatttacgg aattgtgcgc ggatttggta
    18481 gctttgtccg gaactatctt gttcaagtgt attgaaggtc acgctgcttc ctttatatga
    18541 tcctttgata ccaaaaaggt tatgatttgg ttcgcttgat aataagctgt ttccagatcc
    18601 actttcaagg atagcttgag ccatcatgac tgatgcgtag attccttctt tttgcccgat
    18661 ttcctgtgca tcatctgcaa tctttttcaa gaactgatca gttgtttggt tttgactgaa
    18721 atggaacgag ccatctaatc catctaaaat agatgagccg cctgtcagcg aacctgaagc
    18781 agcaggtaaa cctgctaagt ttactcggac agcaaaatca ggcgtgaaat ctgaaataga
    18841 agttactcga actttatctc ctggttgagg cgcatggata aattgtccgt tcccaatata
    18901 gatacctaca tggtgagtaa caccggaaga aggtccccag aagacgaggt cgccaggttg
    18961 ggcctgactt actggaatct gcgtacctgc atattgctgt tcgcctgtcc agccaccgat
    19021 ataatgaccg gttgctttca tatatacgta ataagtcaaa ccagaacagt caaaagtatt
    19081 tggacctttt gctccccata catatggttt tccaagatgc ttgaatgctt ctcgaacgat
    19141 tgcttgttgg acattcgttg tatttgtagg caatacagca tcaattggtg attttacaac
    19201 aaaatcagaa gaagcagaag atggattctc tataccgctt tgatttgctg tatttggacg
    19261 agatgaagca ttgttcgttc cgccattcga tgaaccattg tttggatggt ttggttctct
    19321 ctgagtattt ccattgttat tcgatccaga tgggtttgtt tgattcgaag aattcccgct
    19381 gttcgaatta ttgttattat tgtttgaatt atttgaacca ttattattgt tgttattacc
    19441 cacatctggt ttgtttggcg tttccggatc tggtgtacta gggacttctt cactaggatc
    19501 agttggtttc tctgttgaag tatccggtcc agctttaaat tctgcagtaa tagataaatt
    19561 gttcgtacct actgtaaagg tctttgcatc ataatttact tctttggcac cattaacagc
    19621 gactgaagca atttgttcac cttcagctgg cgtaatagtg taagttacct tcgtttccgc
    19681 taataaacct tctaccgttc cactttccgt aatatccaaa ggttgctgtt caggggaatc
    19741 cgctgtcgaa gcagtcagtt ggatcttacc aatcaaagaa ggagcaattg taagggaata
    19801 tgtctcggct ggcttttcat tttctgatgg atcagccgga tcaatttcag ttggctcggt
    19861 cgatcctgtt gttgagttag ttgttccagt tgggtcttct gtaaaagaat ccgttgtttc
    19921 actagtagct gagctggatg tagtcgtatc tgttgtgact gtttgatcag ctatttgatt
    19981 atctacggca aatacggtat tggtcgacaa aacagacgga gcaatcagcc aagtagcagc
    20041 tagcaaacgt atcgtttttt tatttttcat aataaactag tccttttcct ttcattgatt
    20101 ttctgtctta ttcagcattc attagacatt ttttggaaca gataatcgtg tcgaaaagct
    20161 ttttcttttc ataaatctgt ctgtgtattc taaataaaac aatttgtaaa taagcaacga
    20221 gcattcattc gtttttcttt gacgttcatt ttattgcata gaccaccttt gtttggggat
    20281 aaacaagtgg ggataaaaat ggttatgcat atgtaaatta taccaaagtt tggaaagtat
    20341 cacaaaaatt tataaaaata atgatcctaa aaagaaaaag actgaaatat gtgataaaca
    20401 tctcatactt cagtcttttt cattaactat tcataagaca ttaggtcttc atcaaacccg
    20461 ctaacgagag tagtgatcat tgctacgact agactaacag ccagtcctgc taagtaccag
    20521 aaaaaagtac cggaaatgaa tgcaggcaat gtggtcagac taccaaatac aaaagcgatc
    20581 gctttcactt gggcaaatcc cattacagct ccaccagcag ctgcaccaat gcaagcagca
    20641 acaaatggtt ttttcaatgg gatcgcaatt ccgtaaacag ttggttcggt cactcccgca
    20701 agtaaaatcg aagcaaaagc agaaccggag atcgaacggg tttttgaacg tttacttcgc
    20761 agaaatacac ctaaagctgc accagccatt ccaaaattgg ctgctcctgt caacggaacg
    20821 attcggtcga acccataagc agagatatta ctgatcatga cagggtttac agcccattgg
    20881 ataccaaata ttaccaaaat attccaacca ccagcgatga ttgcgccact gatcggtccg
    20941 ttaatatcca tcatccaagt aattgcggca gctaaacctt cgccaaggta aacacccgca
    21001 ggcccgaata ccattaacgt caaaggaacc gtgataacta atgaaagtaa aggtacaaat
    21061 actaattgca tattttcgcg aatatatttt ttgaggaatt tttcaacaaa agaataaaag
    21121 aaaataccta aaatcgccgg catgacagta gacgtatact gcatcagtac tacaggaatc
    21181 cccagaaaat cagtagctgc tttgccactg tctgaaagta aagaggtcag attcggttca
    21241 atcagtgctg cagcaatgac agccgcaata tatccattca ctttaaacgt tttggcagca
    21301 gaaaaagcaa gaaagattgg cagaaaataa aagacggcat tcgacgcagc agacaaaata
    21361 tgataagttc cgcttgtttc actaagccag ccaatcgtag tcgctaaaac aaccagacct
    21421 tttaatatac cagaaccggc aaacagcgga atgatcggcg taaagatttc cgatatcaaa
    21481 ccaaatagtt tttcgatcgg atttagcttt tctttcggag ctgaatgatc gatttgacgc
    21541 gtcaattcct tgtaagcagg agccaatccc tgatccatga ttatttctgc atatctatcg
    21601 agtaaaatga aatacttaat ggaagggatt tgctgtttta atttttctgc attggctact
    21661 ggctgttttt tcaatttgat acgaagccga gtcgcacatg gaaggacaga atcaatattg
    21721 tcttttcctc ccaactgttc aatcagcata tgaatctgct cggacagtac catctgttca
    21781 ggattcattg tcgtttccat tagaagacca ctccactcac taatataata ttggcgtaag
    21841 gtgtcatttc gcctgtacga ataaatgcat gacagttatt caactctttt ttcatttctt
    21901 catgtgggat aaagctaatc ggtgtatctg gtaacaataa tttgattttt tccaatattt
    21961 ccgggttcat tgtctttatt tcttccgcca attcaatcga ttgtacctca agttcttcta
    22021 aaacgttttc taaaacagat aaaaaacttg gaagaccgtt tgagacagcc aaatctattt
    22081 tttcagtacc aaaggggaca ggcatgcctg catccccgat acttaattta tcaaagtgcc
    22141 ccatttgtgc gattactcgt gaaatatctg aattgattac ttttcctttt ttcatgtttg
    22201 tttcttcctc tcattgttaa ttttagactg ctgattgttc taaagcttcg cggtaaggga
    22261 tagaaggttg cgcaccataa gactgcacag caacagaaga ggctagattt ccataaagga
    22321 tcgcttcttt caagttacta aggtctggct gaagtgtgct gcttaatgcg ccaataaatg
    22381 tatcacctgc ggctgttgtg tcaacggcat ccactttaaa agcagggacg ataccgtgtt
    22441 ctttttctgt atgatagaaa gcgccggcac tgcctagtgt aatgattact gttccaatac
    22501 ctaattcatg gagtttttcg gctgctgcga ccaaactgtt atgatctgtc actctgattc
    22561 ctgtaatgat ttctgtttct gtttcatttg gtataatgat atctgtcttt tcaagtagtt
    22621 cagtaggaat cgtttcacga gctggtgcag gattcaggat cgttgttttt cctgcgtctt
    22681 ttgcaataga aaaagctagg atcgtcgcat ctaagctact ttcaaactga gcaataataa
    22741 agtcactgtt tgtaatcagt tgcttgtttt ttaggacttc ttgttcatgg aaagcattat
    22801 ttgcccctgc atggatcaag atactatttt ctcctgctgc gtctaccata atcattgctt
    22861 gtccggttgt ggcgccttgg atttcagcta tcccagatgt atcgatgttt tcttttgtca
    22921 gtagatctaa taactgttgt ccttcgctgt cagccccaac gcctccaata aaagatgtcc
    22981 gtgcgcctga acgtttggct gccactgctt ggttggcacc tttacctccg ccagcgtgaa
    23041 aaatctcatg cgcatgcatc gtttcgcctg gcttgggcat gttggttaaa cgaagggtag
    23101 tgtccatatt aatacttcca attactgtaa ccgtgttcat tttacaaacc tccaactatc
    23161 tcatttattt tgatgggaaa attatttttt aaaacgtttt agtaaaacgt tttaatagtt
    23221 cgataaaaat atagcacacc ttgaaagcta tttcaagggt gcagttgaaa atcttttttc
    23281 taatcgtaca ggtaatcttt tttcttccca ttctttttct gggaattgga tccgatctaa
    23341 caataacttg gcagatgttt gtcccaattc gaagattggt tgtgcgatcg ttgtcagttt
    23401 cggcttgata tactcgcaca tatctatatt atcatagcca ataatgctgt agtcttcagg
    23461 gatgcttttt ccagcttcct ctaatccgcg atataatcca aaagctaatt catcatttaa
    23521 agcaaacaca ccagttgcag cagaatcaag aagttctgct gtgatctggt agccaccttg
    23581 ttttgagaac tgtgtaggta agaggatcag ttggtcgtga gggatttggt aaacatcaag
    23641 agcagattta aacccctcaa tccgagcgtg gacattttcc ggtggatttt caggatagac
    23701 aagggcgatc gtttgatgtc ctagactaag caagtgcatg cccgctaggt aaccaccgcg
    23761 aaaatcgtct gttcgtacgg catcactaaa tccttcagat tttttctgat ccaaaacaat
    23821 gaagggccgg ccttgttttt tcaaattttc gttgattgca tctgtagaga cagcagaagt
    23881 cgcgataatg aatccatcca cacctcgacg tgtgagctcg gctagatatt cgatttcttt
    23941 ttgatgatca gaatcagcat tacataatat cgtgacgaaa ttctgcttat acaagatatc
    24001 ctcaattcct cgcatcagtg tactgaagaa tggattcgtg atatcaggaa cgagtacacc
    24061 aattgttttc gtttccttgg taatcatttg tctagcaaaa tagtcaggct gatagcctaa
    24121 ttcttctttg gcagctaaaa ctttctgaac agttttcgga ctgaatttct ccgtatttcc
    24181 atttaatatc aaggagacag ttgtgattga aacgccggaa agtttagcga cttctttgat
    24241 tgtgatcttt tttttcacca tgttcatact cctatctaaa tcctgtggtt attttcccat
    24301 gaaatcaaaa agaagtaaat gaaaatcacg tgtgccagta cttatttgct ttgttggata
    24361 gcttcgatta tttgtttcgt tgagcgtact cgtgcagttt tcggaaatat cttttttatg
    24421 aacaattgat gggattcttt gtcacgatca ctgcaagcat cttcagcaag cacgatatgg
    24481 tacccgtgtt gaaaggcatc taatgcagta gcatatacac cgtttgttgt cgctacaccg
    24541 cttaaaatga tagtttcgat tccacgccgg cgaagttgaa gatcaagaga agtaccaaaa
    24601 aaagcacttg gattgtattt tgtgatagtc aacatattat ctgtatcttt caaagaatcc
    24661 ttcaaaagta aatcagtgaa ttctggaggt acagaagcag tcattccacc catatcagtt
    24721 tgttgactca aataagggaa agtggagatg tctacgttca ctaaaacttt caaggcagcg
    24781 gtacgtttca atgttcttac taacaaatca ttgtttgtca gaatagatgc tgcagtgtgt
    24841 ggagagagtg taccgttatt tacgatctct ttttgcatgt cgatcgcgac aaaagcagtt
    24901 tttgataaat caaaaatcat attttccttt aaatcattca tgatgttcct tctttcaaat
    24961 aacaatgttt tttatgtaag taaatcataa atcgtttatt tgtcaactaa attaaaggat
    25021 tcctttcttt agtctagaga aattcagtgg taaaataaat aagtgtgaga aaacgcattc
    25081 gatgcataga tatagcgaga aatatcgtga aaatggagga atcagagtga agtatgtcga
    25141 agtaaaaaat gaatacaaac gatacaaaat gggagaaacg accattacag caaatgacga
    25201 tatttctttt tcaattgaaa aaggcgaatt agtgattata cttggtccaa gtggagcagg
    25261 aaagtcaacg attcttaata ttttaggtgg gatggataca cctgatgaag gtcagatcat
    25321 tattgatgac acagatattg cgcaattttc agataaacag cttactgctt accgacgaac
    25381 agatgtaggg ttcgttttcc aattctacaa tctcgttcct aatctaacag caaaagaaaa
    25441 tgtggaattg gcaacggaag tttctcctga tgcgcttgat cctgtagagg tattgaggca
    25501 agtgggccta gctcatcgat tgaataactt tccgtcccaa ttatcaggag gcgaacagca
    25561 gagggtttca attgctcgtg cactagctaa aaatcccaaa cttcttttat gtgatgaacc
    25621 gacaggcgcg ctggattttg aaacgggcaa gcaagtattg aaattattgc aaaacgctag
    25681 cagacaacac ggcaataccg ttttgatcat tacacataat tctgctttag cgcccatcgc
    25741 tgatagagtc attcacatta atgatgctaa agtaaggtct gttgaattaa atgatcatcc
    25801 ttcatccatt gatgagattg tatggtaggg ggattttatg aaaacaagaa catatcttaa
    25861 agcaggtttt cgcgaaattc ggcaatctaa aggacgattc attgcaatca tccttattgt
    25921 catgctagga acacttttat ttgtaggtgt caaaactgct ggacctgtca tgcaaaaaac
    25981 gatggatcac tatgttggta cagcaggact ttctgatttg caaatcgttt ctactggtgg
    26041 cttgacacaa aaagatattg ctcaagcaga aaagatccca gatgcaaaag tagaaacagg
    26101 aaaacagatc tattattcca atactggcaa aaacgaagtg attcaaatat tttcttataa
    26161 caaaaataac aagcagaaca agctacaatt aactgatgga aaactcccgg aaaaaccaaa
    26221 ccagcttgta ttggatgaaa aagcggaaga tgaagggtat aaaatcggtg atatttatca
    26281 gattgattca gatgaattga aagaaaaaga gtataccatc acgggctttg tccgttctcc
    26341 attgttcatc aacaatttgg agagaggata cgcgaacgtc ggaaatggta gcgtcgatta
    26401 ttttgtctat ttacctgaat ccgtttttaa aacagatatt tattctgtct tatatcttga
    26461 ttttacaaac gtaaagaatt tgaatacgta cagcaaagct tacaaagata aaatggagca
    26521 aaatcaagaa aaggcagaaa agtatttaca agatcgaccc gaagaacgct tagaagagct
    26581 gaaaaaatcg gccaacgaag aattagaacc ggcacagcaa aaaatagcgg atggcaaagc
    26641 acaaacaacg caagcaagaa cacggttaga agatgctaaa aaacagttag cgcaacagtc
    26701 agcaactatc cagcagcttc ctgaagcgca acgaacaatc gctgttcaaa cattatctca
    26761 acaagaagct caacttgctg aacaagaaag gcaattagtt gagaaagaac gtgaactggc
    26821 ctctgcagag gaagaattga atcgaaatgc agaaaaaatt gaaaatttaa ctaaaccatc
    26881 atacctatac aatgaacgtt cagaaaatgt cggttttcaa gaatttggtg atctgtctga
    26941 tcgtatcgct gcgattgcca atgtttttcc tgttttcttt ttctttattg ccgcattgat
    27001 cacctttacg acaatgacac gtatggtaga agaaaaccgc agggaaatcg ggacattaaa
    27061 ggcgttaggt tatacgaaag ttgaaatcgc aaaaaaatat gcaatctatg caagtctagc
    27121 ctcagggata ggtattgttt taggaacgat attaggaacg aatcttttgc caaggatcat
    27181 ttttgagctg tctaacgagc ggtatgatat tgggagtgcg cttatttttt atgactggcc
    27241 gccgatttta caagcagctg ccgcattctt cattgccgca tttggcgctg caatgatcgt
    27301 tttattcaaa gatttaagag aacggcctgc tgcattgctt caaccgaaag caccgaagcc
    27361 agggaaacgc atttttcttg aatacatcac tccgctgtgg tcacgcctta gctttaatca
    27421 aaaaatcagt taccgaaatc tcttccgata taagtcaaga atgcttatgg cgattatcgg
    27481 aattgctgga tgtgccgggc tgatggttgc aggcgtggga ttgaaagatt ctctaagttc
    27541 tgtttcagcc aaacaatttg gaccgatcat tgactatcaa gcaatcgtta cattggattc
    27601 atccacggaa gatagtcgtc aaaaagcagc ggatatgctg cagaagcaag aaaaagtaaa
    27661 tgagatccta gctattcata ctcaaaccgt agaattgaga aaaaaaggac atgcttcgca
    27721 aagtctgaca gtgatcgtac caagtaaaac agctgatttg gatcgtttta tccatttaac
    27781 tgatcttgaa gagaacccac gctccttgcc taaaacagga atcgctatta cgcaaaaagt
    27841 agcagaacta tttgacttat ctacaggaga tcatatttcg ctttatgatg aagatcaaaa
    27901 agaatggaaa gtcgaagtaa aagaaatcgt gaaaaactat ctaggtagct ttatttatat
    27961 gtcccctgaa tattatgctt cagttactga tcaatcgatg atggataatg catttttgct
    28021 gcgtacagat aagatgacaa caaaagaaga agaaatgctt tccgagaaac tgcttgccac
    28081 agacgctgtg acaaatacgt ctttcatgtc caaacaaatc gaaacgcaag aagaatcaat
    28141 ggcgaactta gactcggttg tcttaatctt tgtcgtatta tcaggactgc tggcttttat
    28201 cgtcttgtat aatctaacta atatcaatat ttccgagcgt gtacgcgaat tatctacaat
    28261 aaaagtcctt ggattttttg ataaagaagt cacgatgtac attgttcgag aaaatatcat
    28321 ctttactctt ttaggaatca tcggaggatt tggtgtagga tatatattga ctgacttcat
    28381 cttgcaacaa gcgtctatgg agaatgtcat ctttccatta gtgatcacat ggtgggctta
    28441 tgtcttatca gcaggattaa ctatcgtatt tacggttatt gtgatgattg ttacccattt
    28501 caaattaaag catatcgata tgattgatgc gttgaaatcg aatgaataaa aacagaggtt
    28561 gtgaaatcag cggtttgacc tgagtattag agaaacaaac ggcaaaatag tttttcatat
    28621 tttgccgttt tttgagctaa taccgaagga cagctgattg aacaccatga atcagaggtt
    28681 gtaaaaaagc cgtcctgcat cgagcaaaaa gaatactcaa ataaaattag ctcctcatgt
    28741 tttacatttg agaagactga ttgccgtaga taaaagcttt tgaacaccat ttatagaaga
    28801 aacaagaata ttttgctatt ttaaacaaga ggatagacaa atacatgtct gatctcttgt
    28861 tttttattgg gcaacagacc tcttagaatg tataaagata aattttacaa acgctttcgg
    28921 ttcagttata ataataaatg ataacttgta tatataagtt gtggtaggta ataaatcagt
    28981 tgaatggagg taaatatcca tgacatttaa agacaaagtc atttaccaaa tctatcccaa
    29041 atcttacaaa gacagtaatg gtgatggaat tggagattta aaaggaatca aagaaaaact
    29101 cccgtatctg aatgatctag gcatcgacat gatttggcta aaccctattt atccaagccc
    29161 tcaaaaagac aacggttatg atatctctga ttacacagcc atcgatccta tctttggtac
    29221 gatggaggaa ttcgaacagc taatagccga agcaaagaaa taccggatag aaatcatgct
    29281 cgatatggtg ctgaatcacg tttcgacaga acatgaatgg ttcaaaaaag cactagcagg
    29341 ggaccagcat tatcaggact tcttcatttt aagagaagag ccaacggatt gggtatcaaa
    29401 atttggtgga agtgcttggt cgccatttgg agatacgggg aaatattatt tgcacttata
    29461 tgatcggaca caagctgact tgaactggcg gaaccaagca gtaagagagg aactattcaa
    29521 agttgttcgt ttttggatgg aaaaaggggt aaaaggattc cgtttcgatg ttatcaatgt
    29581 tatcggtaaa gacgaacaat tgaaaaataa taaagaaaat gatggcaaac ctgaatatac
    29641 ggacaaacca atcacccata cgtatctaaa agaattgaac caagcaacgt ttgggacgga
    29701 ttccactatc atcacagtcg gtgaaatgag ctctacgacg atcgaaaatt gtatcttgta
    29761 tacagaacct agtagagaag aattatcaat ggtctttaat ttccatcatt tgaaagtcga
    29821 ttatgaaaat gggaataaat ggtctactcc tccatttgat tttgcagaat taaagcgact
    29881 atttcataca tggggagaaa agatgagtga gggtaacggc tggaatgctc tattttttaa
    29941 taatcatgat cagccacgtg cgctcaatcg atttgtagct atcgatcagt atcgaaaaca
    30001 aggagcagag atgctagcaa ccatgatcca tttgaaccgt gggacgccct atatttatat
    30061 gggagaagaa atcggcatga tcgacccaga ctattcatcg atttccgact atattgacgt
    30121 agaaagtttg aatgcttata agttactcct tgaacaagga ttcacagaaa aagaagcgtt
    30181 tcgtcggatc aaagcaaaat ctcgcgataa ctctcgaaca cccatgcaat ggtcagacac
    30241 agaacaagca ggatttacta cagggaaacc ttggttaaaa gttggaggca agctagaaga
    30301 gattaatgta gaaaaagaac tatcttctga agattctatt ttgtcctatt acaagaaatt
    30361 gattcggctg agaaaaacat atccaatcgt tgcacaagga gattatcatg cttatgaagc
    30421 aaatcatccg gaagtttatg gctttcttcg ccaatttgaa ggacagcagc tgcttgtatt
    30481 aacaaatttc tatgataaag atacaaggat cgccatccca gaaggattca cagaagccaa
    30541 tattcttatc gataattatt ccgatactgt tcttcatcaa agtctggagc taaaaccata
    30601 ccaggcaatt gctttgctga aaaattaaaa caaaaacaca aaacgtcgtt cctacgagct
    30661 attctcgtaa gatcgacgtt ttgtgtttta tttaaaataa tcttcttcag aaaatgaaga
    30721 gttttcatac tctcctttat cgccataaaa tttattgtag cgttgtttga atttacggaa
    30781 aataaaagta acgagtaatt tgactaacgt atagatagga ataccaaaaa taacgcccac
    30841 aattcccatt agatcaccca tgacaagtaa aacgattaag atcgtaatcg gatggatctg
    30901 taaacgatca cccattactc gaggaatcac caagtcaccg tggagaagtt gtacggcaaa
    30961 ccagacaatg acaaatttga cagccataaa agtagagtct tggaatgcaa tgatcaaacc
    31021 tggaaagaag gcgataaacg gcccaatata tggaatgata cataacaacc cagcagaaat
    31081 agctaaaaca ctagcaaatt ctaatccgat cagtaagaaa caaacccagt agactgctcc
    31141 aaggataaat gaagcaatga tttgcccctt tagaaatgaa cccaattgtt gattagcaat
    31201 ctttgtcaaa tttttaaaat ctgtacgaaa tgctggtggg acaatcgcta aaactgactg
    31261 ataaaatttt tgaggatctc tcaaaaggaa aaaagcgaca attggaccag taaataaagt
    31321 aataaaaata gctgtaacag ttgaaaaaat gttgctgatc cgttgtgcgc cactttccca
    31381 ataatttcca ataaatccag ctatatcaga cgtcacgtcg tcaatagaag cgaaaaattg
    31441 attaaatgaa tcggcaaaag gtgttttctc caaaaattgt cggagactct cttgaaaatc
    31501 ttgaaacaaa tctggcaaac tactgatcaa tgtctttgcc tgttcttcaa gaaaaggaat
    31561 aagccatatg cttccaagcc ccaataaaaa taaaataatc acataaacga taatgactga
    31621 caagttcctc gaaaggtgct tgctcaaacg tttgatgact ggttccaaaa gataatacaa
    31681 gatcagtgca aacaataatg ggggcaagat agtagcgata atgattccga caggcttgaa
    31741 tataaacgag atctgatgga aaaaataaat gatcagcgca agtaagataa ctattcctaa
    31801 aacatagtaa gaggtttttc ctcctaaaaa gcggatcaaa cgattcgttg tatctgtttc
    31861 ttttttcact ctttttcctc ctttatctat aacttcatta tagacaaaag cgggtataaa
    31921 aggaaaatga tagaggttga ttgcaaaaat atcgtcaatc attcatataa actttctcat
    31981 caattaaaat aagaaaacgc ttgacagttc ttttaaccat atgataaatt aaatcctgtt
    32041 gctaaaacgt aactattacg gtttgcgaaa agcgaatcgg aacattcaaa tgaaagaaga
    32101 tgagaagagg agtgtcataa atgaaacgat tgataggtat attggccatg ctggttttag
    32161 caggtatgtt aactgcttgc ggggcaagtg ggaaagccga ggacagtaaa gaaaaattga
    32221 gcgtgatgac gaccttttat cccatgtatg attttacaaa agcaatcgtc ggggatgaag
    32281 gagaagtaga gttattgatt ccagcaggga ctgattccca cgattatgaa ccttctgcta
    32341 aagacatggc aaagatccaa gatacagata tctttgttta taatgatgaa aacatggaaa
    32401 catgggtccc cgcaatccaa aaaacattac aagaaggcaa tgtccatacg ataaaagcga
    32461 cagaaggtat gcttttactt ccgggaagtg aagaaggaca tgatcatgac catgaacacg
    32521 gggaagaagg ccatacacat gagctagacc cacatgtatg gctagctcca agtttagcaa
    32581 tcaagcaggt agcaaacata cgtgatcagc tgattgaagc ttacccagaa aaacaggaag
    32641 tatggacaaa aaatgcagca gcctataccg agaaacttca agcactccat cagctttatc
    32701 aagaaacctt taagcaagca aaacaaagat cttttgtcac gcaacatgct gcattcaact
    32761 atctagcgtt agaatatgga ttgaatcaag tatcgattgc tggtctttct tctagtgaag
    32821 aaccgtctgc ggcgagaatt gctgaactga aacactttgt taaagaacat ggaatcaact
    32881 atatttattt tgaagaaaac gccaaagata gtattgccag aacgttagca aacgaagcag
    32941 gtgtatcttt agaagtattg aatcctttgg aaggattgac gaatgaacaa atagaaaatg
    33001 gagaaaacta tctttctatc atggaagcaa acctcgaagc gttgaaaaaa acgacagaga
    33061 cagaaaaccc gctagaagat actttgaccc ccaaaaaaga aaagagcgtc tacaacggtt
    33121 attttgaaga cgaggatgta caagatcgct ctctttcaga ttgggcagga acttggcaaa
    33181 cagtagatac gtatgtagaa gatgggactt ttgatcctgt ttttgaatat aaagaaaaat
    33241 tgaatcagga taagacagca caagaatata aagaatatta tacgaagggc tatcaaacag
    33301 atattgcttc aatcaagatc agtgagaaag agatggcatt tacgtttaag aatggaacaa
    33361 cgaaacgttc aaattatcgt tatgtaggga aagaaatctt gacctacgag gcaggaaacc
    33421 gtggcgtaag gtatctattc gaagcagaag ataaggaaag tggcgctttt agatatattc
    33481 aattcagtga tcacacgatt gcaccggctg actccgaaca ttttcattta tatgcaggaa
    33541 atgaaagtca agaagtattg ctgaaagaaa tggagaattg gccaacattt tatccagata
    33601 caatggatgg acgtgaaatt gcacaggaaa tgcttttgca ctgataggca aaaaagaacc
    33661 gaagggtttg gatcggctgg tcatttagtc ggtccaaact attcggttct ttttatgaaa
    33721 tcaatttctt taatccacgg atagaggctg ggatggtctc gccgtttgcc aacgtgactt
    33781 ctctggtttt tttattaatc gaccgcacat tttgtgtatt gataacatag gatttatgac
    33841 aacggataaa attccgatgc attttctcga tttctttgat cttctcatag aattgtaatt
    33901 gggcatgagt caaatggact tccagtttat gtggcgctcc tagcgaagta gaaaaatatt
    33961 ggatgtcttt gatcggtaaa aatttgatct catcattaaa ctctattttg aagatatctt
    34021 tttggatttg ggacatttga aaatcttcga aaattttgct caggatatgc tcgacatttt
    34081 cttttaaatc ataaagatct tctttgacga tataatcagt cggttcgatg ttgcttttta
    34141 agattttcat cgacatttcc gtatgggaag tcacatagat tatttttcct aaaggatcta
    34201 attcacggat caatttaccg atcgctacac catccatttc actgtcttct agttcgatat
    34261 ctaaaaagta aataccaaaa gaagaagttc tgatcttggc tgcattgaga agttcttccg
    34321 gatcagaggt agaaatatgg acataactgt ctaagttttc aatcatgatt ctatttttga
    34381 tcaccgttga taaaacttct aattgttttg gatcgtcttc acaaatataa atgggaatca
    34441 tatcattact cctttatttt atttgtatat aaatcgtttg agaaaaacgc tggttttcaa
    34501 ttgatgtttc aaggaaaaga taaggattgg tctttgtcaa ttcatccaag ataaacaagc
    34561 caaggccttc gttttttgga tttgtttttg ttgagaaccc tttttgtttc aactggttta
    34621 acggtaatgt atttgttgtt gtattgatca cacgaatcat cacggaatga ttcttcttga
    34681 gtatggtcaa ttcgattttt ttcttctttg cagcactagc tccttcaatc gcattgtcca
    34741 agataatacc aagcatacgt acaaggttta ccgtatgttt cgtatccagt tcgattggct
    34801 caggaaaatg aagctggacg atcaaccctt tttcctgtgc catcatgagt tttaatgaca
    34861 gtaaactacg gatctcagga actttcatat tatccaacat acttagattg gccggaaaaa
    34921 ggttcatcat ctggcgtgta ggtaatatcg tttgatcata ataagaccgt aatccttcta
    34981 agtcgccttc tgaaatatag ctgtgaagag aaaaaagcag attttggtaa tcatgtttga
    35041 attctctcaa gtcttgatag tttttttcta gatctttgat ataatcctgt tgcatcctga
    35101 cttgttgctc taataactga tcagctaatt tcttattaga gattttccaa taataaacca
    35161 agatgatcag gccaacacac agatatagaa tgaaaaaact cgtattcaaa gctaagacat
    35221 caggtgtttc accagaaaaa cgggtataaa aaataatgat atagtatgta aataatatca
    35281 atgtcccgat cgtaccgatc atacggcggt ccacagtgtg aaaccaagtc tctaacgatt
    35341 tttttaaaca aaaagctaat aaagcagata aaaaagcgga taagacaatg tgaaaatact
    35401 gcaatgaatc tcctggttca ataaccgaag atttcaaaac aaaaaaatcg ccgatgcttg
    35461 ttaagtgatc tcctaaaagc gacgtcaata acccaaaagg aagcgcgtaa accaattgtt
    35521 gacgcgaagt cgaaaagaag aaaaaagtaa gcatcagcat tcctatagca attagagcag
    35581 taaaaatccc taaaatcgaa tagacccatc caatcaaaat acaagaaatc agaatgactc
    35641 ccgcaaacag atatttgtat cggatgtgat ggtcaatcaa taagatacaa attgtgtaaa
    35701 tcaaatattg caacgctatg gaccataaaa acataaagta cagctcccat tgatttttac
    35761 caaattatag cacaattggc gtattatttt tttaattttt cagggatttt gaattcagaa
    35821 ctcataccaa agcagcagcc agatacaaaa aagtcagata agttagttaa ttgtttaatg
    35881 attgtttttt tcatgaaaga ttcctccaaa attcatcaag tagtttgtaa taggaagcaa
    35941 cgtagctatt tctaaaacaa tcccaagtag caaataggta gaaaagtaag aagctgagaa
    36001 aaataacgta atgagagaaa aaattagaaa catgctgatc gccttcttta ataatggggg
    36061 acgtttctcg ggttcaatgg gattgattgc tgtacctctt gatccatagc gaagcagtaa
    36121 caaaaagcag ctgattaaca aaaagaccat cattgggaag gagatagtca attgcagatg
    36181 tttcatcacc aagggaacgc cccataaata aagtagtgtc cacagcagac aaacgaattc
    36241 agagtcggaa tgcaatccat aagcatatct gcggatgcag atataagaaa gaaaggtgat
    36301 tcctgtttcc gcccatgagc ctgtcaccag agaaaagact agaacgaccg ctagttttcc
    36361 aagattattc aataaaactt ctaagacaaa ttttacttgg acatacacca tatcttcttc
    36421 cgatttgtct ccgctgaata aggcttctaa taatttgttc acaactttat ctttcatctc
    36481 atacgtacct ccaccacaaa ttttatgcta aaaaaaataa aaaacgtctt ttgtttcata
    36541 aaaagtggtt ttgacatcaa aaccgtcatg atctcctaaa aaaatcttcg ttttccaaga
    36601 accttaccaa aaaaagtgac agttttgata cgaaaagtac tttttatgac aagtttgtgc
    36661 tttttcgagt gatttatgtt aaaaaattac tgaccttata aaaggtgatg gagggattct
    36721 tatggaaaat gaacaattgt ttgaagtcgg cgaaacatat gcatttaaat ctgatttatt
    36781 tgaatcacca ataaaaggaa aaatagaaaa aatctatgaa aatagtgtga tgatacgtgt
    36841 catgaaatgc gcagtagaag acaatgatac cgctcataat ctgcactggc agttagttgt
    36901 acgcaaatca agtacaatag aagaataaaa acagataaaa tgttgagagc ataaagaggt
    36961 tgtgagtctc ccgtttagct ctgagttaca aggaataatg ttaaaaaaca gcttttcatg
    37021 ttttatgaat attgccttgt gaccgaggtg ttggcaggag aatactgttt atttgaaaaa
    37081 agggctgcga caagactttt gtcacagcct ttattttcat gaataggagt tacttttggc
    37141 tacttttaga gaagtagctt ttaaggagca gataaagtca aggcttttat ttctataaat
    37201 gaagtagtaa aatgaagaac agattttatt acaggaagga tgccgataat tgtgatcatt
    37261 gttgcaggaa cgatcggtgc agggaaaagc acactaactg aaatgcttgc ccaagattta
    37321 gaaacaaaac ctttttatga gaacgtggaa gataatgaag tattgccgct attttattct
    37381 aatccagaaa agtatacttt tttattacag attttctttt tgaacaaacg ctttttggca
    37441 atcaaagatg ctttcagcca tgatgacaac gtactagacc gctccatcta cgaagatagt
    37501 atgctgtttc atttgaatgc agatttaggg cgtgtttctg aagtagaagt aaagcagtat
    37561 gaaggtcttt tagaaacgat gctgaaggag ttagaagaaa tttcgccaca aaagaaacca
    37621 gatctactgg tttatattcg tgtatccttt gaaactatgt tagcgagaat caaaaaaaga
    37681 ggccgggaat atgaacagct agaacaagat cctgaactct attcctacta taaagaactc
    37741 aatcgacgat acgaagagtg gtatgaacag tttgatatat gtcccaaaat agtgatcgat
    37801 ggggacaaat atgatttcgt agccgatccg gcatgtggag aacaaatcgt acaagaaatc
    37861 aagatgcggg caaagaaaat gacagaagag gcagatgccc agtcgatccg ctacactaca
    37921 aataacccga aagcaaaggg aatctttaca actaaataat ataaaaacta agcaaattgc
    37981 tcactcgcag atatttctct gtgagcagca tcttgcttag tttttttgat tcattgagat
    38041 caaatagtga accgattcgt atgataaaat tgccataaat gaaatgtctg agtgagggcc
    38101 atgatcaaaa ataaaatgga aacgatcaaa atcgaatggg cgccaaacag atgatgacag
    38161 aaaacaagga caatagctcc tgcgataaag gcaagaacga cacttagata gtggaaaaat
    38221 cgatcaaaat ctgctttttc tttagaaata caataatcat ataagttcgt tgctacccct
    38281 tttaaatttc cagttgtaaa taaattagtg taggcacgcc cgttgatttt atcaaaagct
    38341 acccactgga tcgatgcagt aaaagcaatc aaaatcgtta ctaatgcggg atgctcatgg
    38401 acgggtgtca aactaacaaa gaaaaaaatc actgcttcat aatatagaac aaacaagcgc
    38461 cagaaatgtt tttctttttt tctaaaataa tcaattagat actttgttag aagaatccct
    38521 aagaaaaaga aaagggtaga caatactttt ttaccaacac ttagccattc tccgtcaaat
    38581 gcttggatga tcgatagaat gatattgcca gtttgagcag aggcaaaagc accatattgg
    38641 atataagtat aactgtccat ggctcctccg ataaatgtca gcaaaagtcc tattgtacgc
    38701 ccctcgtgaa aggaagtctg catttttttc atcacctttt atgaatattt ttgtcttcct
    38761 tatcttagca taaacgattg aaaaaaagga aaaattttca gaaaactaaa atcatatcgc
    38821 ttcccatttt catttttaga aaggtctgtt agtataaaga aaaaatagac aacgaaactt
    38881 taaataacca acttgaaata aacaaattca aagaagaaag atgggaagga aatcaatcag
    38941 ttgaatgaaa aagaaattct ccgattacaa aaagaattga aacgctggga gaacaagtat
    39001 aaaaagcttc aacaagtctt tcggctacaa gaagaaatca ttgctaccta tgaagcggct
    39061 tatggcgaag cacccggact tattgaggaa gaagtacaag taaaagaaag tgaaggaaac
    39121 agctctttct ttaaaatgat ggagaaacgt tcctataatc gcttcaatca agaacaaaaa
    39181 gcagctatca catatgatat gaagaaacat cttcgaatca tagcaggagc tgggagcggc
    39241 aaaacgcaaa caatctgtgc aaaagctgtt tatctgatga cgcaaaaaca agtagacgaa
    39301 gaacgcatac taatgatcac ttttacaagg aacgctgcaa atgaactaaa aaaacgagta
    39361 gataatttca gccagagaaa aaccgctgtc catatcggaa cattccatag tatctttttt
    39421 aggatataca atgaaatttg tcgaaaattt cctgaagtag caatgcaagg aatacaagga
    39481 gacttttcaa aggatactgc acaaaaagtg aatgctgttt tgcaacagct gatacgtaaa
    39541 tataatcttt atatatttga tcaatatgga gaaaaaacga ttgcttctcg gttagactat
    39601 tggcagaata tgaacctttc agtagaagaa atggttcagt tagttaaaga aaaatatgat
    39661 tccattgata agaatgcacg acagccgata tcagaacgac tgtatggatt gctcacggaa
    39721 ctccaagaac aaaaaagaag ccagcaattg ctagagttca atgatgtact ccaaaatctg
    39781 aaaagcgctt tggagaatga ggagatccag cattatatcg gccaaaaata tgattatcta
    39841 ttcattgatg aatttcaaga tactaatcca cttcaatggg aaatcgtgaa acggatgaca
    39901 aaggaaaaca ggatcaagtt gattgttgtt ggagatgatg atcagagtat ctatggattc
    39961 agaggatcag agccagcata catcaagaac tttgaaaaag aatatcctac caaaactctt
    40021 ttcttattaa ctaattatcg ttctcgggca gctattgtcc aaggagcaaa tagattgatc
    40081 agctataata aaaatgatcg gataccaaag gcgatgattc cagcacaaaa agaagcaggt
    40141 gttatggaag cctatttgtt ttctgatact aaagcagaaa gtcagtggct aatcaaacaa
    40201 attcagacat ttattcaaaa aaatgggaaa tataaggaat caatcatatt ataccgatca
    40261 gcttcacaaa cgcagcagct tgtccaatct ctgctgaaaa cgaatattcc ttttgtactg
    40321 gaagcagata gtccatatga aggtatcttt ggtatcaagt ctttccaaca tttctatcaa
    40381 aaaattgttc attggcaaac agcactgaat ctgcaaaaga aaaatcaggc ctaccagcaa
    40441 ttgctccggc agctgatggc agactgctac ctgaaaaaaa gtgaatgcga tcaatacttt
    40501 tctcctaaat atcaaaatat agccgttgca gattatattt tgtcgataag ccccaattta
    40561 aaatcgaaat ctgcagaaat ccgagagttt gaggaagcgt tgcgtaaaac agaccagcat
    40621 caagaaatct attcgcttgt tcaaagctat cttcatctgc cacgtatagc taaggaaatc
    40681 gaccagagtg atcaagaatg gatacgagaa gaagtaaagc agcatgctac tttcagaaga
    40741 ctccaagcct tatcgcaaga aacccaagcg acagctaaag aattaaaaac ccgcttgatt
    40801 gcttacagac aaggaaaact tgatgcacta tgtatccaga gcattcacaa aagtaaaggt
    40861 cttagttacc gaaatgtctt tttgattggt tgtaacgaag gaagtttgcc ttataacgga
    40921 gcgacagaaa agaaagtaat cgactttaat gaaataaaag cagaaccagt aacgacgata
    40981 gaagaagaac gaagactatt ttatgttgcg atgactagag caagaaatcg tctgtatcta
    41041 tgtgtacctc aaatgaaaaa ttcaagaaaa ttgaaagtat cgagatttat caaagaaacc
    41101 gggagtcgtg tgaaaaaagg aaagtgatag aagagagggt gtgacagaag tggtcagttt
    41161 caagaaagaa gaaggaatgt acgaaaattg cttctcaaat ttttgtgaaa tttcagctta
    41221 ttttcgaaga agctatttct ggaacacagt ttattcggaa aagggaggtg tgacaagaat
    41281 tttgtcacac ctcccttatt tattttgaaa atgagaatac tattatcaaa atagagaata
    41341 tatagaaaaa ttctcattat tcaaacgtaa ataatcagtt attttgattt tttttatatt
    41401 ttattagcta ataattggta ttttaaaaga aattactgta gaattggatt ataattaaaa
    41461 aggagtgaga aaatgataaa agtatatact tcgcctagct gtacttcctg tagaaaagca
    41521 agagcatggt tgcaagcgaa tcaacttgaa tttgaagaga agaatatttt tgccgaaccg
    41581 ttaacagaaa gtgagatcaa aaagatcttg gcagcgactg aaggaggagt tcaagatatt
    41641 atttccactc ggtcgaaaat ttacgaaaag ctgggcatag attttaacga attaacttta
    41701 aagcaaatga tcgtcttatt caaagaatat ccttcattat tgagacgacc tattttgatt
    41761 gatgaaaaaa gaatactaat cggatttaat gaagatgaga tccggtcatt tgtccctcgt
    41821 gaaattcgat gtgttgccag aaatctgatc tatttacaaa acaataacat cttaaattca
    41881 tagggaggga gccttcgtgg aagtgaaagt ctatggatca gcgcactgtg taacaagtct
    41941 gaaggcgaaa caatggttag aacaccatca attgccttat caatttgttg acttagaaga
    42001 acgcgatcta agtacagaag aagcagaaga attatgcagt tttgaagaag tgcaagcaga
    42061 acagctattt gcgacttggt ctgaggcttt tcacaacatt gcgattgacc tttcctgtgt
    42121 aggaaaaaaa caactgattt ttttatgcac acaacatacc aatctcctca gacgtccttt
    42181 gattattatc gatgaagttc tatttattgg atacaatgag cagttgatgg aacagcttat
    42241 cttaaataaa tagactttaa ctgttttaat caagtcactg agcatttcat acagttgtaa
    42301 cagtcgaaca cttagcatca agcgtgttat gtgttctttt tttgacttta tttttctact
    42361 atagagacat gctcaattta tgtggtcaaa attcaaatct aggtggggat atagagttaa
    42421 aaatatattg acatgtgtaa atttacatga tatattcatt tctgttgttt taagtagcaa
    42481 agcaataaaa tagtatttta aaaaacatat aaaatagttg ttgacatgtt agaaatgaca
    42541 tgataagata actgagttgt taaaaaagaa ccacttgtga agcttgattc tatcgaaaaa
    42601 aacttcaaaa aagttcttga caaataacaa atgataagtt atgataaatg agttgctgta
    42661 agatttcgaa aaacgaaatc aaaaaaactt ttaaaaaaga gttgacaatt acttgtctct
    42721 ctgatatgat ataaaagttg ctacggcagc caaaaacgaa tcggg
//
LOCUS       DACEOM010000016        40631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEOM010000016 DACEOM010000000
VERSION     DACEOM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.017"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(69..1952)
                     /locus_tag="IW781_000838"
     CDS             complement(69..1952)
                     /locus_tag="IW781_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1181854.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2218..3093)
                     /locus_tag="IW781_000839"
     CDS             complement(2218..3093)
                     /locus_tag="IW781_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1181855.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(3202..4971)
                     /gene="aspS"
                     /locus_tag="IW781_000840"
     CDS             complement(3202..4971)
                     /gene="aspS"
                     /locus_tag="IW781_000840"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1181856.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(4986..6278)
                     /locus_tag="IW781_000841"
     CDS             complement(4986..6278)
                     /locus_tag="IW781_000841"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1181857.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            6672..7619
                     /locus_tag="IW781_000842"
     CDS             6672..7619
                     /locus_tag="IW781_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1181858.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(7708..8967)
                     /locus_tag="IW781_000843"
     CDS             complement(7708..8967)
                     /locus_tag="IW781_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1181859.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9138..9584)
                     /locus_tag="IW781_000844"
     CDS             complement(9138..9584)
                     /locus_tag="IW781_000844"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1181860.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(9595..11808)
                     /locus_tag="IW781_000845"
     CDS             complement(9595..11808)
                     /locus_tag="IW781_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1181861.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(12041..12805)
                     /locus_tag="IW781_000846"
     CDS             complement(12041..12805)
                     /locus_tag="IW781_000846"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1181862.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(12806..13753)
                     /gene="prmA"
                     /locus_tag="IW781_000847"
     CDS             complement(12806..13753)
                     /gene="prmA"
                     /locus_tag="IW781_000847"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1181863.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(13766..14254)
                     /locus_tag="IW781_000848"
     CDS             complement(13766..14254)
                     /locus_tag="IW781_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1181864.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(14220..14891)
                     /locus_tag="IW781_000849"
     CDS             complement(14220..14891)
                     /locus_tag="IW781_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1181865.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            15029..16315
                     /locus_tag="IW781_000850"
     CDS             15029..16315
                     /locus_tag="IW781_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1181866.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            16387..16581
                     /gene="ssrS"
                     /locus_tag="IW781_000851"
     ncRNA           16387..16581
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW781_000851"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            16659..16943
                     /locus_tag="IW781_000852"
     CDS             16659..16943
                     /locus_tag="IW781_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181867.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            17016..17495
                     /locus_tag="IW781_000853"
     CDS             17016..17495
                     /locus_tag="IW781_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1181868.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            17718..17861
                     /locus_tag="IW781_000854"
     CDS             17718..17861
                     /locus_tag="IW781_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1181869.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            18439..20022
                     /locus_tag="IW781_000855"
     CDS             18439..20022
                     /locus_tag="IW781_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1181870.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20459..21643)
                     /locus_tag="IW781_000856"
     CDS             complement(20459..21643)
                     /locus_tag="IW781_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1181871.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(21668..22675)
                     /locus_tag="IW781_000857"
     CDS             complement(21668..22675)
                     /locus_tag="IW781_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1181872.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(22847..23206)
                     /locus_tag="IW781_000858"
     CDS             complement(22847..23206)
                     /locus_tag="IW781_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181873.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(23178..23549)
                     /locus_tag="IW781_000859"
     CDS             complement(23178..23549)
                     /locus_tag="IW781_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1181874.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(23566..23868)
                     /locus_tag="IW781_000860"
     CDS             complement(23566..23868)
                     /locus_tag="IW781_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181875.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(23885..24355)
                     /locus_tag="IW781_000861"
     CDS             complement(23885..24355)
                     /locus_tag="IW781_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1181876.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(24330..24608)
                     /locus_tag="IW781_000862"
     CDS             complement(24330..24608)
                     /locus_tag="IW781_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1181877.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(24605..25654)
                     /locus_tag="IW781_000863"
     CDS             complement(24605..25654)
                     /locus_tag="IW781_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1181878.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(25611..26540)
                     /gene="cpaF"
                     /locus_tag="IW781_000864"
     CDS             complement(25611..26540)
                     /gene="cpaF"
                     /locus_tag="IW781_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1181879.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            26672..28426
                     /gene="ade"
                     /locus_tag="IW781_000865"
     CDS             26672..28426
                     /gene="ade"
                     /locus_tag="IW781_000865"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1181880.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(28524..29186)
                     /gene="trmB"
                     /locus_tag="IW781_000866"
     CDS             complement(28524..29186)
                     /gene="trmB"
                     /locus_tag="IW781_000866"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1181881.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(29256..30035)
                     /locus_tag="IW781_000867"
     CDS             complement(29256..30035)
                     /locus_tag="IW781_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1181882.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(30242..31453)
                     /locus_tag="IW781_000868"
     CDS             complement(30242..31453)
                     /locus_tag="IW781_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181883.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(31446..32183)
                     /locus_tag="IW781_000869"
     CDS             complement(31446..32183)
                     /locus_tag="IW781_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181884.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            32321..32755
                     /locus_tag="IW781_000870"
     CDS             32321..32755
                     /locus_tag="IW781_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1181885.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            32748..33092
                     /locus_tag="IW781_000871"
     CDS             32748..33092
                     /locus_tag="IW781_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1181886.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            33272..34282
                     /locus_tag="IW781_000872"
     CDS             33272..34282
                     /locus_tag="IW781_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1181887.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(34351..35295)
                     /locus_tag="IW781_000873"
     CDS             complement(34351..35295)
                     /locus_tag="IW781_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1181888.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(35279..37999)
                     /locus_tag="IW781_000874"
     CDS             complement(35279..37999)
                     /locus_tag="IW781_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1181889.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(37996..39216)
                     /locus_tag="IW781_000875"
     CDS             complement(37996..39216)
                     /locus_tag="IW781_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1181890.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(39497..40342)
                     /locus_tag="IW781_000876"
     CDS             complement(39497..40342)
                     /locus_tag="IW781_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1181891.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        12035 a         8438 c         7091 g        13067 t
ORIGIN      
        1 aaaacacggt aaatataaaa aaacttcagg caaaaacaaa tgtttttgcc tgaagttttt
       61 tgtagctatc attctgtcac gtttgtctca ctgctgttcg tacttgaact tcctcctagc
      121 aaggcttcgc tgactaagtg aatgtaatgt gtcatccctt caggatttgg atggacttga
      181 tcatcataaa accagtcact atggtcatta ctgtagctgt accaatcaat cagcgtcata
      241 ttgtcgtatt ttttcgtcat ctgttcaagc atcgcattca cgtcattttg ccagcgcttc
      301 gtcggtactc ggacattgac ccagtatact tttcgatcac cgattgcatc catcacacta
      361 tcaaactgag cttccgagaa tgagccattc gttcctagtc cgatcagaac agtgtctttc
      421 aacaaatctt gttcctttaa tttttcaatg tacggtacac tggcatataa ttgtcgtccg
      481 acatctccat ctaccactac ttttggataa agttcttgta gatccgttgc agcatccaac
      541 ataactgaat caccaaatgc cgttagttct aatttttgtg ccttattccc ttgtgccttg
      601 gacaactcgt acttatcaag gatcgcttgg tcaaccgaac tagtggtgtt ttctgaattg
      661 tttctggttt cttctgctaa ttttttactt tcagcgattt ttgcctgcag ttgtttttga
      721 tcggctgtta cagcgttttt cggtgccgta acgaaaccaa cgagtgcaat tgctacaagt
      781 aatgtgcctg gaacaaccca tggtttgctc ttactgatga tcggtttgct gaaccatcct
      841 ttcatcactt ggaatgtacg accataatca aaacgtgcta gcggacgctc cacatatcga
      901 taagaaagtt cactgatgat tagaatcaat gtgatttcca ctaaagtatg caacaaaaca
      961 tgatcggcaa tatcagtcac ctttgcttca taaaaaatca taactggaaa ctgatacaga
     1021 taaatgccat agcttcgttt gccaatccaa gtaaatactg gatttgtcag ccatcgattc
     1081 aaacttgctc cggggtgtgc tgtcactgca actagcacta cgcataacag actcactagg
     1141 agcattcctc cgtaatagat gaaattgtaa tgatcatcta aaaagaagaa gcaaagaatc
     1201 agtgccaaaa gcgagattcc tccagcgata ttcagaatat ttttcgcttg tttcgggatg
     1261 tttttcttta aacgcgtact tggccaaaca aacgcaagcg cacttcccaa ccatatagaa
     1321 aacagacgag tgtcggtacc gtaataaaca cgggttggat cgcctcctgg tgtatacaag
     1381 atcatcatca agatggcgga gaggaaggat gtacctaaca cagtataaaa gatccatttt
     1441 cttttacgaa caaataccat caacaataca aacaagatcg gccaaaccaa atagttttga
     1501 ccttctactg ctaaagacca gatatgcgta aacggtgact cattagcaaa tcgatcgaaa
     1561 taagataaac cgttattgat ttgccaccag ttgttgacat acagtaagct gctgaccaca
     1621 actcctcgaa gattgtttaa cagccctcgc tgaaacaaag tgatataagc cgaagctgtc
     1681 acaagcataa aaacaagtgc cggatagagt ctcttcattc ttctgacata aaattgccaa
     1741 atattgattt ttccattttg ttcccattcc tgccgcagca gatccgtgat caaataacca
     1801 gatacgacga aaaaaatcgg aacgcccaaa tagccgcctt tcatactagt tggcagaagg
     1861 tgatagaaga taacacccac cacagctaat gaacggagcc catcaaaccc agtgatgtag
     1921 cggctgtttt ttaacctttt ttgtttttcc attcaatatt ccaacttttc ataaactttt
     1981 ttcattcgta atatacgggt attaagtgaa aatagcaagt agaatcgtgt cctttctcta
     2041 ctattttttt ctatcatccc ataaatttaa aaaaataaac atgctttttt tcaactttaa
     2101 gaaaatttca aataaaaaag cagtttttct ttgctgattc tactatggta gaaaaaaact
     2161 gcagacaaac gaacgtctgt ctacagcagt atttcatgaa tttttgtcaa actcgatcta
     2221 aatgccatcc tcatagaaac gaccaagtat ctggacgttt tctgcaatac ttacaaaagc
     2281 ttgtggatca gaagctttca ttgcttctct aagtagaggc agttcgtatc gtgtaacgat
     2341 cgtcatcaat actgtctgct tatcatgctt ataagcacct tctgcttcat tgatgatcgt
     2401 gatccctcta cgcatacagt tttggatttc atttactact ttttctggat gtttcgttac
     2461 gatcattacc tgcatcttct tttgtttagt ataaacgaca tcagttactt ttccactgac
     2521 gaaaatagac aacgtactgt aaaacatata ttgccagcca aatagaattc cggcaacaaa
     2581 aacgatcaat gcattgaagt agatcgaaat agaaccaatc gactttcctg tttttttacg
     2641 aatcgtgata ctcacgatat ctagaccgcc cgatgatagg ccgtttctca acgctagccc
     2701 aatacctagt cccatcacag cccccccaaa caacgcacag atgattggat cggtagatag
     2761 gactgtttcc ggtaaaatat ggatacaaag ggaggtcaaa gtgactgtga taaaagtgaa
     2821 aattgtaaac ttcttcccga tttttaacca cgccaataca aacaaaggta tattcagtaa
     2881 atacaaagtt gttgataccg gtacttcgaa tcctatcagt gattttgaca aagtcgtcaa
     2941 aatctgagct agaccagtaa taccacttga ataaatatgt cctggctgat aaaagaaatt
     3001 caatgcgact gatgcgatca acgcataaac gatagatacc gaaagcttgg tcgtgtaatc
     3061 atgataagcc caattttcga atgttttttt catggaaaag ccccgcttta tatgttatgg
     3121 cttcattata aagtgttttt caattaaatc aaggttgtca aaagaagcgt attcgacaaa
     3181 tcctttttac ctttttatcg gctattcttc tatattcgtt acatcaatat tcaattcgaa
     3241 caattgtaaa ggcgaaacca tacttggtgc atcactcatc acatctgctg cttttccgtt
     3301 tttaggaaag gcaatgactt cacgaatatt atcttctccg gctaataaca ttgcaaaacg
     3361 gtccaatcct aatgcaatac cgccatgtgg agggaatccg taatctaaag ctgttaacaa
     3421 gaaaccaaat tgttcttcca tttgttcttt tgtaaagcct agagtttcta gtactttttc
     3481 ttgaagctca cgagtatgaa tacggataga acctccgcct agttcataac cgttcaagac
     3541 gatatcataa gcttctgcat agacttttgc agggtcttca gccaataacg ggatatcttc
     3601 ttcttttggc atcgtgaatg gatggtgagc agagacatat cgaccttctt cttctgaaaa
     3661 ttcaaattgt ggccaatcag ttacccataa gaaattgaat ttcgattcat caattagtcc
     3721 taattctttt cctaaacgtg tccggatagc acctaatgca gcagcgacga tttcactttt
     3781 atctgcgcca aacatcagca agtcacctgg ttttgcatct gttgctttaa tgattgcttc
     3841 tgttgcttct cccatgaatt tagcaattgg acctttcaat ccgtcttctt ctactttcag
     3901 ccatgcaagc cccttagctc cgaattgtcc tacatattga cctaactgat ccatgtcttt
     3961 tcgtgagtag cgatctgcag cgcctttagc attcaatgct ttcacgacac cgccgttttc
     4021 aagagccatt tggaagactt tgaattctac ttctttgact gtatcgctta gatcgatcaa
     4081 ttccatatca aaacgtgtat ccggtttgtc gctgccgtag cgagccattg cttcatcata
     4141 ggtcatacga ggaaatggca atgtgacttc gataccgcga acttctttca ttaccttcgc
     4201 aatcaatccc tctgtgtaag tttggatttc ttctgctgtt aagaacgttg tttcaatatc
     4261 tacttgcgta aattcaggct gacggtctcc acgtaaatct tcatcgcgga aacaacgaac
     4321 gatttgataa taacggtcga atccagcatt catcagcaat tgtttaaaca attgaggaga
     4381 ttgcggcaac gcatagaaat gtccagcatg gacacgtgaa ggaactaggt agtctcttgc
     4441 gccttctggt gtagatttcc ctaggtaagg tgtttcgata tcgagaaagt cattatcatc
     4501 taaataatga cgaatcgctt ttgttgtcgc atttcttaat ttgatgtttt tagtcatttt
     4561 tggacgacgc agatccaaat aacgatattt caaacgtatt tcgtcactga ttgcttgatc
     4621 atcttcaatt tgaaatggcg gggtttttgc ttgattcaaa atcacgattt cacttgcaat
     4681 cacttcgaat tcaccagtgg ccattttagg atttactgca ttttctccgc gtcttctaac
     4741 ttccccagtc acttcgatca cgtattcact gcgacattta tccgcgattt cccatgcttc
     4801 tttgttgatt tcaggattaa agacgatttg aacgattcct tcacgatcac gtaggtcgat
     4861 aaagattact cccccaaggt ctcgtctttt ttgtacccat ccttttaatg taactgtttg
     4921 ttctaattgt tgttcggaaa ctaatccgca atagatcgtt ctttgtgcca tttatttttt
     4981 tcctcctact cgaataaagt catttcatca tagacttcat caaaatgttc atatatctct
     5041 tctaatggaa aacttttttc ttttcggctt gccattgctt tgacattaat cgtttgacta
     5101 gcaagctcat cttcaccgat caccaatact aatttggcat tcgctttatc tgctgtctta
     5161 aattgcgctt ttgctttacg gttcatgaag tcgcggtcag ctgaaaaacc aaagtttcga
     5221 atcgcttgga ccactttcaa tgcttcgata tttgtttcgt ctccgagtgc gaccacgtaa
     5281 gcatccaact cattaaatgc agggataact acttcctctg cctccatagt caagagtaat
     5341 cgctcgatac ccattgcaaa tccgaagcca ggtgttgaag gaccacctag ttcttccact
     5401 agattgtcat aacgtccgcc tgcgcaaatc gttgcttgtg cccccatttt tggtgcgtca
     5461 ctcatgattt caaaaatcgt atgtgtgtaa taatctagtc cgcgaaccat attactgtcg
     5521 atttcataag gaacagccaa tgcatccagc atggaaacaa cggtatcaaa atgtttttga
     5581 gcaggctcgc ttagataatc taagatagat ggagcttctg ccacaaattt ttgatctcgt
     5641 ttgtctttac tgtccaatac gcgtaatgga ttttcatgaa ggcggcgttt agagtcttca
     5701 ctcagctctg attcaaaagg taaaagatag tcgatcaatg cttgacgata ggcttgacgc
     5761 gtttctttat cacctaatga attgatgact agccggattt gcgtgatccc caactgtttg
     5821 aagaaatcaa gtgccatcac catgctctcc acatcagttg cagggttttc tgatccaaat
     5881 gcttctactc cgatttgatg gaattgacgt aaccgtcctt tttgagggcg ttcgtagcga
     5941 aacattggtc ccatataaaa agttttgaac ggtttcgcat actctgggcc gaacaattta
     6001 tgctcaacaa aagaacgaac gattggtgct gtaccttctg gtcgaagcgt cacatggcgt
     6061 tcccctttgt cataaaaatc atacatctct tttgaaacga tatccgttgt atcccctaca
     6121 cttcgggata tcacttcata gtgttcaaaa atcggtgtac gcatttcatt atactgataa
     6181 tctctgaata ataaacgtgc tgtttcttcg acaaactgcc atttttctga ttcgcctggc
     6241 aaaatatcat ttgttccttt tggtctttgg aaactcatag ttttctctcc ctttcttagc
     6301 tatgtaaata aaaaaatcac ccttgttcat catgaacaag ggtgaatgaa attcatacac
     6361 ggtaccacct aattttgaaa gatttctctt tcctcttacg aataacgctc gtacgcggga
     6421 cggcttttca ctcgtccatc tccagagtgt ctttaggcta atgaaacttt agatcatttt
     6481 cagcctcgat gatcctctct tgtaaagttt caaaaactta ctcgctcttt cattgacatt
     6541 cgatattact tataacctac taaaaaccag tctcaaagtc aagagatatc tgactatttc
     6601 ccctcacaaa aacgttttag ttcagctttt tggtattttg ttttagacta gacaaaaagg
     6661 aggaatcagc tatggcagaa ataagaatcg gacattcaca agtctacgca gaacagcttg
     6721 gattaggagc aaatgccgtt ggaggacata atctttttga cggtttggaa gatgagacag
     6781 gaaaacaagt agttcgtaca gctttgaata gcgggatcaa tttgatcgat accgcttatg
     6841 cttacggaaa cggccgttcc gaagaactga ttggcgaggt actgaaggaa aaagaatatg
     6901 atcgctcacg tgtcgtcatc gctacaaaag ctgctcatgt accaaataaa gggcgaacat
     6961 ttgataattc tcctgagttt ctcaaacaat cggtggaaga tgcactaaaa cgtctgcaaa
     7021 ctgattacat cgacattttt tatatccact ttccggatga aagcacacca aaaaatgaat
     7081 cagttgctac attgcacgaa ttaaaagaag caggaaaaat ccgagcagtc ggtgtgtcaa
     7141 acttcacgtt agaacaattg aaagaagcga atgcagatgg atatgtcgat gtagtagaag
     7201 ataaatacag tttgatccat cggcaggctg aaaaagaatt attcccttat cttgaaaaaa
     7261 acaaaatcag ttttgttccc tattttcctc ttgcttccgg tttgctgaca ggaaaatatg
     7321 aacttggtga ggaaaaacaa tttggtgaag gagatcctcg aaaaagaaat ccagatttcc
     7381 aaggagaacg tttcagagaa attttaaccg ctgtcgatgt acttcgcccc attgctaaaa
     7441 gataccaagc cactcctgcg caacttgttt tagcttggta catgaagaat ccacgagtgt
     7501 ctgtagttat acctggagct aagcgtccag agcaagtatc tgacaatgtc caagctttag
     7561 atttgcattt atccaatgaa gattatcaaa ccattgatga agcatttcgt ggattttgat
     7621 caattgacct tatcaattta tagtaatatg aaaaaaggaa gatcagcact gtttgatctt
     7681 cctttttttc gataagttta tgctaatcta attcttgtaa agtagcgctc ggctattcgg
     7741 ataagtcgga aactgattga atggaatcgt ccaatcttgt tcaggtacag agaactcaaa
     7801 cttatttact agctgatcgg aaaacacacg tagactatga agagtgatcc attctcctgc
     7861 acagcgatgc atttgtcgaa aatttccccc gccttgtgca atcatctcat attcttcttt
     7921 ataggaaata tcttttgctt tccctacata ccgtttgatc ataaaggaat ctggcgcttc
     7981 aactgttcgt tcatcatggt ctgttccata taaatctaaa ataacccaac tgccttctgg
     8041 tatacgataa ccatctactt ctacttcttt cagcgaaatc gcaggtagca ttgggaagaa
     8101 aggataatag cgtctcattt cttgaataaa cggatcttgt aacgtcgaaa agtcattttt
     8161 taattgctga tacaaatctg gtctactgaa aagtgcatgt cccatcaatg ccgcccacac
     8221 agttagagca accgttggac ggatgatgtt caataattca acagctgcca cttccagagg
     8281 taaaagctga ccatctaaat ctgtggcatt ggcaaaagca tataatgcca cattttcttt
     8341 tccagccaca ggattttgtc gagcttcttc aatcaagcct tgcgcccatt tttctgattt
     8401 tttgcgattt tcgacacctt ttatgtgatc aatcggagaa gtaaacgttc cgctgatcat
     8461 agagatttga tattctgcta atttttcgac ctcatcttta gaaatcgctg ataaattgat
     8521 acctgcccac tcgcaaatag atgtaaagaa aaccatctta cttaaatcaa atagttcaaa
     8581 ttgaccatgc tgtgcttcaa gtgcttgtgt cagattctta tctaagatac gatgataatc
     8641 ttccattctt tccggtgtca ttagatccat aaaaatattt ttccgatggt gatgagcagc
     8701 tccatccaaa gtctgtactc caccttgacc aaaaagtgtc ttcaatacta attttggcat
     8761 agcacctttt cgtttgaaat tccttggatc ataaaatact tttgctgctg atgatccgta
     8821 gatcgtgata gcttctttat tgaaaatctt tgctttgacg acgggagcgt ccgcttcatg
     8881 gcgtaactcc tccaacatgt tgtatccttt ttgatacaat ttttttaaat ctgtgatttt
     8941 gatatctact actggtactt ctttcatctt cagcaacctc ctataaaaaa taacttgtat
     9001 aatttaagct taactttttt tccgactgct gacaaaagaa gcgctttttg cagacaaaaa
     9061 agccacttaa gttagagttt atcagtctaa cttaagcgac gtgtttcata gtttttacta
     9121 gtgaaaacca gattacttta tttgttttta gaatcaataa tgatcgttac aggtccatca
     9181 ttgacgagag aaaccgacat atctgctccg aattgtccgg tagcgactgt gatgccgcga
     9241 cttctgattc cttcattgaa tgattcgtat aaaggaatag ctgtttctgg tcgtgcggct
     9301 tccacaaagc ttggtcgatt tccttttttc gtatccgcaa acagtgtgaa ttgagagata
     9361 cttaaaattt gtcctcccac atcttctaca ctaatattca tttttccttg ttcatcttca
     9421 aaaatacgca tttgtgcaat tttttttacc agatagtcca catctttttg cgtatcggtt
     9481 tcatgaatgc ctaataaaac aagtaggccg tagccaattt ttccaatttc ttgctgatcg
     9541 actgacacag cagctttact tacccgttga acaactgctc tcatatctgt cctcctatcc
     9601 attcgtccgg cgtacactgt agacatcagg tactgctttg attttatcca cgatactctt
     9661 taggtgggat aaattctgga taccaaccgt caaatggatc gtcgccattt tatctttatt
     9721 cgtccgtgct tcaacgctgt tgagattttt cgttagcgca ttgactgttt gaagtacatc
     9781 attcaataag cctgaacgat tatagccgta aatttccaaa tctgcatcat actcttttcg
     9841 tgtattcgat gtatcttccc attcgacttc aatcaaacgt tctgctacat ttggtttgtc
     9901 aggctgaaca ttcggacaat ctcgacgatg aatggatatc cctctgcctt tagtgatata
     9961 accaacgata tcatcaccag gaatcggatt acagcagcga ctgatacgaa ttagtaagtt
    10021 ttctacccct tgaatgacaa cgccaccttc atgacgtact ttcattttgt caggttcttt
    10081 tttcggctga ttcatgattt cttctgcttc ttgcttttgc tgctcgattt tttgttcttt
    10141 acgttctttt tcagtcagac gattggccat cgtcaagggg ctaacttctc cataccctac
    10201 agctgcatag agatcatctt ctgtttgata attaaaacga tcgagtgctt cttgcagttt
    10261 gttcttcgtc aaaatatctt taggcgtaaa tcctagatct gtaatacatt tgaccacgga
    10321 ttcgtggcct ttgatcacat tttcttctcg atcttgggct ttgaagaaac gtttgatctt
    10381 atttcttgct ttgctagtag caactaattt caaccagtcg cgacttgggc caaatgaatt
    10441 tggagaagtc atgatctcaa tgatatctcc gtttttcaat ttgtaatcaa gctgcaccat
    10501 tttgccattt acttttgcac cagtggtttt gttaccgata tctgtatgga tactgtatgc
    10561 aaagtccaat ggtccggatc cttttggtaa ctcagtaacg tctccttttg gtgtgaagac
    10621 gtagacttta tcactaaaga tatctccttt tacgccttcc ataaattcag aagcatcata
    10681 gctttcgtct tgaagttcga gtatctcatg gaaccagctt aattgtttcg tcataccatc
    10741 tggttctact ttttcatttt ttccttcttt ataggcccag tgtgcagcaa ccccgaattc
    10801 agcgatttca tgcatttcct gtgttcggat ttgaatttct accggatttc ctgccggacc
    10861 gatcacggta gtatgcaaag attggtacat attagctttt ggcatggcaa tataatcctt
    10921 aaatctgccg ggcattggtt tccattttgt gtggattgct cctaatacag cataacaatc
    10981 cttgattgaa tctacgatca ctcgaatagc tagcaagtca taaatttcgt tgaactgctt
    11041 tttctgatct ttcattttac gatagattga ataaatgtgt tttggccgtc catagatttc
    11101 tgcaaaaatc cccagctctt ctgtcgctat tcgaatatct tcaacagtac cacttacgta
    11161 tttttcacgt tcttctctct tcgtctgcat caagtggacg atacggtaat actgttttgg
    11221 atttagataa cgaagtgctg tatcttctaa ttcccatttg atccggctga tccctagacg
    11281 atgtgcaaga ggcgcataga tttctaaagt ttcctgagcg atccttcttt gcttatcttc
    11341 tcgcaggtgc ttcaacgtac gcatgttatg tagtctatca gctaatttga ccatgataac
    11401 tcgtaaatct tgagccattg ctagcagcat ctttcggtgg ttttctgcta gctgctcttc
    11461 gtgggattta tattttattt tccctaattt ggttacgccg tccactaaca tagcaacgtc
    11521 atcgccaaat tcttctctca gatcttctaa agtgatttct gtatcttcaa caacatcatg
    11581 taagaagcct gtagccactg tatggggatc catttttaaa tctgctaata tacctgcgac
    11641 ttggatagga tgaataatgt agggttcgcc tgattttcta aactgtccgt catgtgcagc
    11701 agtcgcgtat tcgcatgctt tctcaacaaa tgctacatgt tcgggaccca tgtattgact
    11761 aacgagcttt atgacaccgg gtcccgttaa aatttcttct tttgtcacag gaatcgatcc
    11821 ccctttcttt tttcggtaaa acgggaatcg ggacaaatat ctgtttgata attgtcccaa
    11881 aatccgtgca gttatacgtt tttttacgga aatgttcgct ttttaaaagc tttttcaaac
    11941 gatttttcgt atccattgta taaataattg tttctattat acaaattgtt gactattttt
    12001 tcaattcttt ctgtcgatac tcttttatta atgaagaacg ttagctgaaa gaaggattca
    12061 ttaattccca ttgatagcta gcagcactta ataaataaag aggtgctgtt tctgtacgaa
    12121 ggattcttgg tcctaatcca caaagtatcc ctttgtgttc tacaaaataa tcgacttcat
    12181 cgcgggataa tccaccttct ggtccaaaga cagctaagat tcgactatct ggttttgttt
    12241 cactaagaat tttggctgca tttgattttt cgccttgctt tgctgattcc tcataagcaa
    12301 ccaagaccac gtcataatca tctagccgct caaataaatc ttgtttcgtt tccaataact
    12361 ctactgatgg cacgatctgc cgatgggatt gttccgctgc ctcttgagca atcttttcaa
    12421 gccgttgttg tcgtttcata cgtttttttg catcccattt aacgacagag gctgatgccg
    12481 ggaaaccaag aaaagcttgc gctcctaatt cagtggattt ctgaacgatc caatccagct
    12541 tttccccttt aggatagcca ctcgctatgg taatcgaact tggcagttcc ttttgatttt
    12601 cttctttttt aatttctttc agataaacag cctcttcagt aatctccgtg atttctgcaa
    12661 caatggctat ttgatcattg aatgccaaaa aaacttcatc ctcgggttcc atcctcataa
    12721 cccgtacaat gtggtgatag ttttcccctt ctaaaacaaa atagtctttt gattcatatg
    12781 attcttttaa aaaataccgc tgcatctatt ccacctctgg ttttttcaaa ataattgcat
    12841 accagtcttt ttgctggaaa atttgatcca cgataaaccc ttgttctaac atggcatcca
    12901 gaaccatttg cttcttctct tcaatgattc ctgaaacgat cagcgtgcct gtttgcttca
    12961 gtaaacgcca agcatctggg atcatcaata agatgatatc tgctaaaatg ttcgcaacga
    13021 ttacatcact ttcaatctcg attcctgtca aaagatcgtt agctgataca tggacatctt
    13081 tggctatttc attcatatcc atattttctt tcgccgaacg aacggctact tcgtctaaat
    13141 caaaggcata gacatctttt gcaccaagat attttgctgc aatacttagc acgcccgacc
    13201 cagttcccac atctagcaat gtttcaccac cacgcaagac tgtttctagt gctgtcaatg
    13261 tcaaggaagt cgtgggatga gtgcctgtgc caaatgccat accaggatct agtgtgataa
    13321 ttttttctag aggatcttct gtctggtagt tttcccagct tggcgcaacc gtcagcattc
    13381 gcgtaatacg caccggatga tagtatttct tccaggctgt tgcccagttg ctttcctcta
    13441 cttcactaac tgttacttta ttttttccaa tagctagacc aaactcaggt aattgtgtga
    13501 tacgatcttt gataaaaggc aagatttccg gtaagaaagt cgtttccgga aaataagcca
    13561 tgactagtgc gccttctttc agcgatgtaa aatcattttt atctaaaatc tcaccatatt
    13621 gatccgactg aaaatgttca acatctaaag aatcttcaat cgcgacacca ctcgcccctg
    13681 cctccatcaa aatattagca actgcttcga ctgcttcact ttcagtttct acttttactt
    13741 cattccattt catatctgtc actccttaat aacttgggta cggtaaataa gtatctgctt
    13801 cattttgctg ttttacttct actaattctg ctagttgctg attatcccag tgaagctcga
    13861 atacagcttc gtcatcgtct tcatccaaaa aatcaagcaa atcgctttgc ccttgagcta
    13921 atacttcatt tagatattcc actaaagaat cagctactga ttttgccaac cctttttttc
    13981 cttcataagg gattactgct agataattgc gttcatcaaa aacagatttc tgaggattaa
    14041 aaagaagtac gccgtcttca aattcgataa ttggttcttg cgaaacgatc ccttctgcat
    14101 catcaatctc aaattgtgct ttattttctg caaacaagcg aacaacgatc tcgatcgtat
    14161 gattttttgt atcccaatct aaagctacat catagtctgt cacttgtttt tcaattattt
    14221 catctaaata tgttaataga tccttctttt tcattttctt tcctccaacc ccaatctttc
    14281 tcgatttggt tttttctttg taaagagata taaggatttc ccgacacaac aaaacgcaac
    14341 ggcttttctg tccatacacc tttgtttggg ataccgattc taggcaaagc attgatcttt
    14401 tttggtgtct tttttttctc aaaaactaag cgtaacgaac tatctgctat agactgacca
    14461 taaagttctt gaggaattgc taatgcttcc actaattttc ccggaccgtt gctgatatct
    14521 ggaccagttt ttccgccacg atttttactc atttgatcaa tcattgcggc gggttcgatt
    14581 gcacgtatca ttactccttg aggaatccct tctggctgag taatgatgtt caaaatccga
    14641 tgagtatgca tcgtgtaaag gtagatcgta cctggttttt catacattgc ccgcacacgc
    14701 ggtgttcttc ttaaaccata gctatgggcg gcctcgtctt ctggacctag gtatgcttcc
    14761 gcgtctacaa tatatccagc taatcttcct tctggtgtgt catgctccaa atacatcccc
    14821 aataaatccc gtgccacttc tggcgtcgtt ttattccaaa aaaattcatc tatcttttgt
    14881 ttatccgtca tttttctcac ctactttatc atacacaata ttagttggat aagctatgaa
    14941 aacaaaacaa cagaaggctg ttctttctag gcatcttgct aaatatgata taattaaggg
    15001 acgtaaaaac aagaaggagg aaaatcctat gcaacagcca ttggcttatc gcatgagacc
    15061 tcgaacgatc gatgaagtcg ttggtcagca gcatctagtc ggggaaggaa aaatcatacg
    15121 ccgcatggtc gatgctcgca tgctctcttc catgattcta tatggacctc ctggtactgg
    15181 aaaaacgagt atcgcaagtg cgatcgccgg ctctactaat tatgcatttc gcatgctaaa
    15241 cgccgctaca gattcaaaaa aggatctaca agttgtagcg gaagaagcaa aaatgagtgg
    15301 tacagtgatc ctcttattgg atgaagtaca ccgattagat aaaacaaaac aggattttct
    15361 gcttcctcat ttagaaagtg gccgaatcat cttgatcgga gcgactactg agaatcctta
    15421 tattaccatc aatccagcga tccgcagccg tacacagatc tttgaagtca aaccattaac
    15481 agaacaagac atccagttgg cagtcgaaca tgctttaagg gataaagaac gcggccttgg
    15541 acaacaagca atccagttag acgaagatgc tcttcttcac ctttcgcgtg caacgaacgg
    15601 cgatttgcgg agtgcattga atgggctgga actggctaca ctatcgactc cttcagataa
    15661 agaaggaagg attcatctta cactgtcgat cattgaagaa tgtgtccaaa gaaaagctct
    15721 tacccatgac aaaaatggag atgctcatta tgacgttatt tcggcttttc aaaaatcgat
    15781 tcgtggaagt gatgtggatg ctgcacttca ttatctcgcc cgtttagttg aggcaggtga
    15841 tctggcaagt atttgccggc gtctgatggt tatcggttat gaagatatag gtttaggtaa
    15901 tcctgctgct gcggctcgta cagtgaacgc tgtattagct gctgaaaaat tgggattgcc
    15961 tgaggcacgt atcccactag cagacgtcgt cgtcgatctt tgcttgtcgc caaaatccaa
    16021 ttctgcctac atggcattag atgcagcact tgctgatatt cgagagggca aagctggaga
    16081 tgttcctgat catttgagag atagccatta caaaggtgcg aaatcactca accgtggcgt
    16141 gggttatcag tatcctcatc attttgatca ggcttgggtc aatcagcaat atttaccaga
    16201 taaactgaag aatgctcagt attatcagcc aaaagataca gggaaatatg aacaggctct
    16261 gggtcaacag tattaccgta taaaagagtg gaaaaagcat cctccaaaaa actagacaag
    16321 aaaacggttc tgtttctgcg agctctctca ttgctctttt taaattcata tgctactata
    16381 gaggtgggaa atctgtgatg ttcgaattgt cctctttgtg tgttgaccga acatttttat
    16441 tgatatcttg ggacccgtct tgtgtcactg ctggtaacat gccttatcat ttggtagttc
    16501 gaagctacga ccatgggacc cacctgcttg aattcgcggg atcaatacta caggacaaac
    16561 aacggcatta acggagatta ccatctttta aagaataaag caatttgctc ttggcaaatt
    16621 gctttttcta tttgttgctt attttggagg atttgttcat gattaggatt ttaatgatta
    16681 tcgccacttt agtattatta ttcgttagct attatttatt taataaacag gatatctttt
    16741 ttgtattgat caacaaaaat gataaaaatc aaggattcct acaattttac ggagctgcat
    16801 atgctgtttt aggcgtaatg ggtattttaa cagctttttt caatcaacgc tttattgcct
    16861 tggtcttttt actgatcgta attattgttt ctgccacctt cagtatccga tttgcaaaaa
    16921 aaattgctga accaaagcag taaataattc tctttttccc taaagtgttc tacaataaaa
    16981 gagtacaata cgattgactt gaggagtgaa tgcttatgtt acaacaatac aaaaaaatca
    17041 tggttgccgt agatggttct gatgaagctg aattagcttt taaaaaggcg gtcaatgtag
    17101 ctatccgaaa taacggagaa cttctattag cacatgtcat tgacacacgt tctttccaaa
    17161 ccgtttcatc ctttgatgga atgcttgcag aacaagctac tgaaatggca aagcaaacat
    17221 tggcagatta tgaaagcaat gcgaagaaag ctggcttgaa taatgtaacc tctgtcgtag
    17281 aatatggctc accaaaacaa attattgcca gagaaatccc agaagataat caagtagatt
    17341 tgattatgtt aggagccaca ggattgaatg ctgtagaacg tttatttatt ggttctgttt
    17401 ctgaatatgt tattagaaac gcagcttgcg atgtgcttgt tgtccgaact gatttagaaa
    17461 accaattgcc agctaaagac cacaccaaag cttaacacgt agacgacctg ccaactatcg
    17521 gcaggttttt ttgcacaaaa aaccgtgata aaaacaaaag acgcggcaag cactcatcgg
    17581 tctttataat gtttaaaata aagggaaaac agtcaatgag ttcaagtaaa ataaaaaaaa
    17641 gtgaacacca gggcgattag tcgccctggt gtggtttaaa acgacctaag catgccaaag
    17701 cttattggtt cgtcaatgtg gaagtttccg atgaatctga tacgaaaaaa tatgaagtat
    17761 cgatggcgga taccgatttt atcgccaaac taaaatttta tttcgggttc gggtaccgcg
    17821 agaaggcaag tgctacattc ggtaaatcat tcaagaattg atgaaaggct accgcttgcc
    17881 attgcaacca caacgatact cctgaacgcg atgttggtga gatttccaaa gagaagagct
    17941 gtttaatgca acggtattaa gcaaatggca aaaggaagtc atgtagacaa agttttttat
    18001 caaacaccat atgatttcgc ctgaacgctg gtttagctca gaatacaatg atgtggtcca
    18061 cgagacatta ccctcagtca gataaaaaaa gctctctgac agaattacac atcgataaat
    18121 attaagtaaa tgtcagcgct agttgaactt tctgacactt tcaggtacac tggaaaagac
    18181 aactacacga aggagggaaa atactatgat gcgcatgact aaaataacaa gtaatgaaaa
    18241 ttaccaacga taccatatcg catttagcca tgcaattggt tttacagttg ctgttgacgg
    18301 gacaagtttg tccaaaacag ttgaattcac gtaaaatcaa tggccatctc aaggtatttt
    18361 tctcatgata atctgagttt aaataacaca ggccatggat aaccatggcc tttttgagtg
    18421 gtctcaagga gagagatcat gatcatacaa gcaatttcta ttaaaaaaac aatcggcggg
    18481 aatccattat ttgaggattt atcctgtcaa attgaagctg gtgaaaaaat tgccttagtc
    18541 ggtatcaacg gaactggcaa atcaaccttt ttacaaatct tgaccggcag agaaggtgtc
    18601 gacagcggtg ttatcagtcg caaaaaaggc ttaaagatcg ggacggtcga acaagaactt
    18661 acagtttccg aagcgaccgt caaccactac ttgctccatt ctgccgctga gatacaggac
    18721 ctcaaacagc aaatgagtcg ctacgaagca ttgatgacag aaccagatac aaacttagag
    18781 aagtgtctgg cccgttacgg tgagctgcag catcgctttg aagaactagg cggttacgta
    18841 ttagaagacc ggattgcagc gatcttgcaa ggcttaggga ttccacaccg tcaggaagca
    18901 cggttaacag aacttagcgg aggcgaacga gttaaagtcg ctttagccaa aattttagca
    18961 gccgatgccg atttactttt actcgatgaa ccgaccaatc atttagatct taacagtatt
    19021 cgctggttgg aaaactattt acagaataca aaaaaatcgg ttctggtcgt ttctcatgat
    19081 cggcaatttc tcgatcaagt caccacaaaa acatgggaac tggaagaagg tgcattgatt
    19141 gagtatcccg gaaactacag ccgttttcga gtcttgaaag aagctcgctt ggctgagctg
    19201 acaaaaaatt acgaactgca gcaaaaagaa gtccagcggt tgaaagtcat gattcgtcgt
    19261 tttcgtcagt gggcacatga aggagacaat gagtccttct tcaaaaaagc caaagaactg
    19321 gaacgccgcc tggcgaaact gacactcgtc aaaccgccac ctcccccgaa aaatcggctg
    19381 caaagcttgt ctaatggtgg aaaatctggc aaagaagttt tcatcattca aaacttgcac
    19441 cagcagtacg ctgatcaagt actattcaaa gacagcagtt ttgcagtcta tcgtggagat
    19501 catttagcca tcatcggcga caatggtgca ggaaaatcaa ccttactcaa attattactc
    19561 ggtgaggaaa agccttacgg tggcaccatc aaattaggta gtagcttgca aatcggttat
    19621 ttgccgcagc agctgcagtt tgccgatccc gatagccgtt tgctggctta cgccatcaca
    19681 ttgactggaa acgaagaagt cgctcgacgg caattagcca aaagtggctt ttatcaaagc
    19741 gatgtgggta aacggatcaa agatttatcc ggtggtgaaa aaatccgttt ggcgttgctg
    19801 aaactctttt tagaaaaaat caacgtcctg attttggatg aaccaaccaa tcacctcgac
    19861 agctatgctc gagaagaaat tgaagaaatg ctgcaagact atcaaggaac gctcctagca
    19921 gtcagccatg accgctactt tttgcagcaa cacttccaag aagcactcgt tattgatcag
    19981 gagcagatca gccgccagcc gctcggccaa ctgattgact aggacgtttt ttcgcaacaa
    20041 aaaacttgat tttcatacct gtgctttcac gattcaggca aagtacaggt ggaaaatcaa
    20101 gtttttatta ttcgatcaat gaatgtttcg gatcggtttt ctagctaaac ataaagcgaa
    20161 gaataaagtc gacagcccac gaggctgatc taaaaatgtt ttttcttttt acatatgtaa
    20221 tgtcccttat aaagatgaat gaaaataaaa tattgggatt ttcctgaaaa tcaataaata
    20281 aaatgccagt cttttccctt ttgaccactt ttccaagcaa aattccctaa gaagatcctg
    20341 taaaaaagaa gggaaattga agttaaatta ccattttgcg gtcttctccg caaaaaaaac
    20401 acatgcaaga attccctctt gcatgcgttt catcttttat cctaacgaat gctttggctt
    20461 agccgcgcag acgctccaca tcacgggcaa tcattagttc ctcatctgtt gggatcacta
    20521 atactcttac ttttgattct tttgtagaga tatctacttc ttcaccacgc aattcattca
    20581 acgctggatc gatttcacaa ccaaaccaag acataccttt gatgatttca ctacgtacat
    20641 gtccgtcatt ctcgccgata ccagcagtaa agacaattgc atcaacacca ttcatcgttg
    20701 tcacataacc gccgatgtat tttcggatgc ggtccacgaa aatgtcataa gctaaacgaa
    20761 tatgttcttt ttcatagttc ttttcaagat cacgcatatc actagacaat cctgttaggc
    20821 ctagcagacc agatttcttg ttcaaaacat tgatcatttc atcaatatca atctctaatt
    20881 tttccattaa atatgggaga acagctggat cgatatcacc tgaacgtgta cccattgtca
    20941 ccccagctaa tggtgtgaat cccatagaag tatccatgga tttacctcca tcgactgctg
    21001 tgatcgatac accatttcct aagtggcaag tgatgatttt tagttcttcg atcggtttgt
    21061 tcaataattt tgccgcacgt tccgacacat aacgatgact tgtgccatgg aagccatatt
    21121 ttcttacttt gaaatcttca taataagaca ccggtaagct atataaataa ttttgctttg
    21181 gcattgttga gtggaaagat gtatcgaata cagccacact caaaatgtca ggtaaaatct
    21241 ttttaaacgc tttgatcccc attaagtttg caggattatg caaaggagca aattctgcta
    21301 attcagcaat gcggtccatt actttctcat cgatcactac tgaatcccca taagtttctc
    21361 ctccgtgaac aacacggtgc cctactccgg tgatttcatc ataagagctt aaaatttgca
    21421 gttcaatcaa ctgatctaac agcattttta ctgctacttc gtggttttcg atatccatga
    21481 tttgttcgaa tttttgatta tcgccgtact tgatggtaaa aatagaatct tgtaaaccga
    21541 tacgttcgac gatccctttc gcaactacgg tttcttctgg catttgatat aattgccatt
    21601 tcaaacttga actcccagca ttgattgcaa ttgttttaga catatgactc tccttctcgt
    21661 tgaatgatta cagagcagat gatttccagt ttttaaactc gttgaaaaat tccgtcacct
    21721 tctctggttc ttttaatgaa gcaagtttca caagcaagac ttcttttact tgttgtgctt
    21781 gtggtccttt tttctgtaag atcaaaatac ttttttgcga ttgtttatta cggaacaatt
    21841 catctggcaa ttgaatcatt ccttgaagat aaccttcttc tttaaaccat tttttgattt
    21901 cttcactttg atccgtttct aaaaatccac tcggcatcaa aaacaaacca aaaccatccg
    21961 gcttcacata tttcattgat tgctctagca ataaatgatg tgcataacta tggccttctt
    22021 cggtacttgt taaaaactct tttgcttttt cgtcattcgg ataataaccg attggcaaat
    22081 cgctgattgc aaaatcgaca ggatctatta ataattcctg caacccgtct tgatgaaagt
    22141 attgaacatt tgcttgtgtc aaatcacttg tagaagctgc gacagccaac aatgtatcat
    22201 caatatccac accaattcct tctgtttggt accccgcatt cgataggttt aaaagaactg
    22261 ttaataacaa gttgcccatt cctgccgcaa tatccagaat cttaaccggt gcttttttat
    22321 ttgtgtatag ttgttcaata agaaaaacaa ataaaaaacc gatactatca ggagtgagtt
    22381 gatggttcgc ctgcaaatgt tctgtctggc tgcccttcaa taaaagtaat tgagacagtc
    22441 tgcgccattc ttctggctcg aaagaaagtt tcttcaactc ttcataaaga gctgtgatcc
    22501 gttgcgttgt ctcttttgtc ggcactccat ccaccacacg tacttgataa tcatctatta
    22561 aattttctgc attttcgaca taggcttcta aaaaggaagt tcccaatgca ttctgtaaaa
    22621 gctgaatagc ttccaggttt tgattaaacc cttgttccat tttttctggt aacatggtgc
    22681 acctcatttc tagatatatt ataacaattt tttcacctgt ttaattctat cgaacaattg
    22741 acaaaatttc aatcgttttc actgtttttt atttgtgaaa cggcaaacat ttttttatta
    22801 aaaacagtgt tataacagaa aagagggcgt tttcacgaac attccatcat tctgtactag
    22861 tatctgtatc attttttact tcttctgtga accgaaagat aaacggtgag acttccacct
    22921 ctaagaatag ggactgttgt acccactgat atgttacaga tccagttgtg tagtaaaaaa
    22981 ctccttccga actttcggca agcgtagact gttccgataa agccatctgc ttcattattt
    23041 ttgctatata ataatttttg attcgtatat taagatccat tgacaaacgg tagctttcta
    23101 atgctgccat caataaaaac gtacataaaa gaaataaaag caagacagaa aataaaatag
    23161 agcccttata acattttcta ctgatcgttt tttgtccata tggcatatcc atcttctcca
    23221 ttttcaaagt gtacataaaa agaaattgtg tgttcttttt ccaataattg aagctcagtg
    23281 atacttgtta acataggttg atgtccgccg gaagttcttc ttttacgtat cattgcctga
    23341 tatctttcga tgatatactg tttatcctcg atcgtgtagt gcagttgctg atttttcaca
    23401 gagacattgt gactgctctt gagttcttct tctaattgtg ctaaaaatat tagccactct
    23461 ttttgatctt tacgctgcaa aaattcactt acttggatgg tttgctgaat caaaagattg
    23521 aatgtgagca atagactgct caatatcatc agtccaatta gacattcaat cagcgtaaat
    23581 gctggaatta cttgtttttt caatactgat cgcttctttc tccatattga caaacacttt
    23641 ttcgattact ccattattat cgacaatttg gatatcgtat gggatcattt cttcccttcg
    23701 ctctggtctt ttgtgataca tccgctgcgt cttgatatct tcataaatca ttcgaataaa
    23761 tagcagttcc tccccacttt tcttttctgc ctgaagcaaa aacagctggc caaagctcac
    23821 taagtagaca cagacagaag cgatcataag cgctgtaagg gactccaaca aaacaaatcc
    23881 tgttttattc aattcttttt gtatagcgac cactccctat ctggaattga tacgtaatcg
    23941 tccgctcttt ttcttcccaa taaaattgat aagcttttag tgaactttca ttcccggttc
    24001 tagcagcaaa agtaatagaa gcatggcgtt tcaaggtaat ctcatcagga atatctaata
    24061 cttcccaatt tattttatct ggattcggaa catgaaaaat gatttgattc tcttcactat
    24121 tccaataaaa gcctgtttgg atctgattca caatagcgat tttttgcgta gaatagattc
    24181 gtttttcaaa ttggccgaaa aattgataga ctgccaattc attcttccat gcagaaaatg
    24241 cgagaactgg aaaagaaagg atcgttgtaa gtaataaaat cagtaagagt gattcaaaca
    24301 acgtatatcc tgatgctaaa accttacctt caaggccgct gatagatttc tgcatgttct
    24361 cgtttgatat agccttcatt caacaattcc tccacagtag gaactcttcc attattttga
    24421 agcgcataga gctctttttg tgtgtctacc aactggatga tcgcttcacg actttcttgg
    24481 tctacatgat tcctataacg cgacaaatta gggacgaaca acaacactaa tacactgatg
    24541 atcagtaaga cgattagcat ttcgatcaat gtaaaaccgc tataataaga tatttttctc
    24601 tttttcatgg caaaacctcc tctatgcttc cgtaaacagg taataaaaca gcggcataaa
    24661 ctaataaaat caaagccgct acgcccaaaa aaatgattgg ctgaataaaa tgcatccagt
    24721 gatgaagacg tttgaaaagc tgttttcttg tccattcgct gtaaaaaatc agttctttac
    24781 ctaaacttcc ctttgcctct ccctgtaaaa caattttact gaattcttct gatagaaaac
    24841 gataattctg gaactgctca gccaatgaca tacctgcctc caatccttga atcaacttag
    24901 aggcaagatg ctgaatcaga ctttcttgtt tcgtttcctt taagcagact atgatttgcc
    24961 ttaattcgaa accttcatga aaaagctttc caatctctag tgacaaataa gccgattgat
    25021 acaaggaata tatcggtcca atcaataatt gtctgcttat ccattcactc tgatcaacag
    25081 gagacaactt catcagacga taccttacta gtaaagccaa tagagtaacc gctaggatga
    25141 atagtagaac ataccaatga aagacttgta tcagctgaat accccaatgc tcttgcatga
    25201 ccattcctga accaataagc tgaggcaaaa cgaacaaccg catactaaac aagataccta
    25261 gcaaaaaggt gagcaatagc atcggatatg caagcagctt tttcaaatca ttgcgaaatc
    25321 cttcaacaag ccgaaactgt ttggcaattt ccaacaaagt tgtcggcaga ttcccatgac
    25381 gttcagctaa ctcgatctgt accagttgct ctgctccata tcctatttgc gaaagtacct
    25441 cactaaacgc ttgcccttcc tttagctgat gttggatatt tgccaaaata acaggtggaa
    25501 acgaacggat cgtgagtaat aaataaaggg attcttgcag actaaaacca gataaaagca
    25561 gttctcccat ggtttgtgca aactggtttt gctggcttct tgttaatcct ttagaatagt
    25621 tctgactgcc aaatattttc ctgagcttct tcataacttt cctcccatga tttttttact
    25681 gattcttcct ccataaattg ataactccat aaaacagaag gatggtgatt ggcatcttcc
    25741 aaaagttctt gataaatgac accagctaaa cattcagata gctcttcttt tttctttgtc
    25801 agttcatgga ttctgaccaa tgtaccttca agattgcgtg catgtacggt tgcaaaaacc
    25861 cggtgccctg ttagtgctgc tcttaccgca gcatgtgcag ttgattcatc tcttatctcg
    25921 ccaatgatta gcaagtctgg acgatgccgt aatgcgagtc tcaaaagtgt atcataagtc
    25981 tgtgctattt tcggattgat ctgtagctgc agaaaaaacg gttcttctat ctctacagga
    26041 tcttcgatag taatcacttg caatttttct tccaaagcca accgatacat tagagaagtc
    26101 ttgcctgatc cgactggtcc gctgaaaagg tataatccac gtctgacggt tttctgtaaa
    26161 atagttgcaa aatctcgaga actaaagcaa cgatggggtg tttcaaaagt accgtataat
    26221 agccgaatca ctaaactctc tcgttgaagg taatctccta cagaagacaa ccgcagacga
    26281 acttcctgtt tttctaaaga ataagtaaat gcacctaact gagcttttct tttttctccg
    26341 atatccatct gtcccaaata cttaaatctt gagatcagtt gctgtccatc tgccgaagag
    26401 actttcttcc agaatgttcg tttttttcca tagcgaaaat agatgtaaaa tacttctgaa
    26461 gctggcaata tatatacgtc ttgggcatgt ttggcaaaac tttcacttat catttgatcc
    26521 gataatcgtt ttatttccat tattctcccc tccacaaaaa acatacgtct ttttgatctt
    26581 tttgtttcaa ataaaatgaa tttacaaaaa aatattggca aaaaccgaat ttatgttata
    26641 cttataaaaa tttatcttgg gaggcactcc attggaaaca acattaagga aatatatcga
    26701 agtagcaagt aaaaaaaagc ctgctgacct agtgatcaag catgctaaga tcgtcaacgt
    26761 ctttacgaaa gaaatcatgg aaaaagatgt agcaatctgc gaaggaatga tcgtaggaat
    26821 cggtgaatac gaaggaaaaa gcgtacataa tgctaatggg caatatctag tgccaggatt
    26881 catcgatggc catgtgcata tcgaaagctc acttttatca ccaagcgaat tttcaaaagt
    26941 atccttgctt catggagtta ctactgtagt gacagatccc catgaaatag gaaacattgc
    27001 tgggagtaca ggtcttgagt ttatgataga agatgcaaga caaacactca tgaatatctt
    27061 ttttatgcta ccttcttgtg tacctgtaac cccttttgag accaatggtg caaatttaaa
    27121 tgcaaaaaaa ttagcccctt ttttacaaaa acccgaagtt ttaggcttag ctgaagtcat
    27181 gaactatcaa gcagtagcaa ataatgaaac agacatccta gaaaagatcc aattgatgca
    27241 ccagcataag aaaaaaattg acgggcatgc agctggtatc ggcatggaag agttaaatgt
    27301 ttatcctgca gcaggtattc ggacggatca tgaagctaca actgcaaaag aagctaaaga
    27361 acgtttggat cttggtatgt atttaatggt tcgagaaggg accgttgcaa aagatctggc
    27421 atcgctacta ccggcgatca cacctgaaaa ttcaagaaga tgcttctttg tcaccgatga
    27481 taaactgatc aatgatttag taaaagaagg atcaatcgat catatcgtcc gtcaagctat
    27541 tgcacttgga atcgatccat tacaagccat tcagatggca acattgaatg cagcagaatg
    27601 ttttgatttg aaaacactag gtgcgattgc ccctgggtat caagctgatt tcttcttaac
    27661 agacgatctt accaagctgc caattcatga agttttctat aagggaacat tagttgttca
    27721 atctgggcaa ctccaagacc atttatttgc tgatcattca aattcatata cgtgttgtct
    27781 gccaaaacta aacgctcaac cattaaatcc acattcgtta gagctgcttc tttcttcttc
    27841 acaagcccat gtgatcgaaa ttatcccgaa tagtctagtg actaaagatt tggtagaaaa
    27901 agtggaccga caaggcgaac gattcattcc ttcaatcgaa aaagaccaat taaagattgc
    27961 ggtgatcgag cgtcaccatc aaacgggaaa cattggatta ggcatagtga aaggatttca
    28021 gctgaaacaa ggagcgatag cgacaacgat tgcccatgac tcgcataatc tagttgtcgt
    28081 tgggacaaac gacgaggata tgctgtatgc tgctaatctt ttgattcaaa aaggtggcgg
    28141 gatgaccgtt gtcaacaaac agcaggaaat agcttgtgtt tctcttccta taggcggaat
    28201 catgacacag gaaccttttt cgatcgtcta tgaacagttg aatgaattaa taactaaagc
    28261 acatcaacta ggagcagcta aaacctttga tccttttttg acactttctt tcttaacatt
    28321 atctgtcata cccgaattga agatcaccga caaaggatta ttttcatttt ctcgcttcca
    28381 attgattcca ccttgtggaa aaaccgatga aataaataca ttttaaatca aataaatccc
    28441 agaaaataca ttacatctgt aatgttcatt ttctgggatt tatttatgga tattaatgtt
    28501 tttatgaaag cagaaagtta gtttcacaga cttttctttt tggtcttatc ttgaaattga
    28561 gcttctactc gataaatacg ttgacctttt gaagaaaatt tttcttcata ctccgtcata
    28621 atattgcctt caaattgact ttgatgtaaa tctaaccata cctgtttgag gatcatgcca
    28681 tattgagaaa aacttgccag agaatactca aacaatcctt gattatctgt tttgaaatgg
    28741 atttctccat ttggacgaag aatctgttca tctacagcaa gaaaagtctt atatgtcaat
    28801 cgccgttttt catgacgttt ttttggccat ggatcggaaa aattcagata gatttgatct
    28861 acttcgctgt cagcgaaata ctctgtcaac gctgatccat ccacatgtag aagtttcagg
    28921 ttaggaagcg gttgttcgat caacttgtct agtgcaatcg aaacaacact gacctgcatt
    28981 tctactccaa tataattgat ttcaggatgg gcttttgcca ttccagtgat gaactggcct
    29041 ttccccatac caatctcaat gtggattgga tgatcgtttt caaacaattc attccatttt
    29101 cccttccaca atgttggatc actaattaca aaattcgggt gagccgctaa catttccgct
    29161 gctcccggac gatttcggac acgcattatt tatctccatt cctttcctct aattaaaaag
    29221 gtctggccaa atctccgcag agtttgggcc aaacctcagc cactgaaaat tcgttttaat
    29281 tgtaaaattt cttcgttcat ccgtcgatga tcttcacgtt tgcaatatct ggtgatttct
    29341 tggagcaaat tcatggccgc ataccaatga aatttttcaa gcatttcgtc agtcgggcgt
    29401 atgccataac tgatcagcca ctgattccag ctagacagtg gcacataatt tcctaaaatc
    29461 gttgcaatat ccaccgcagg gtctgcaaac atgattgaat cccaatcgac tagatataaa
    29521 taatttttac atactaacca attacgatgg tttacatctc cgtgtactac tgataagttt
    29581 tccttatcta ttgacgggat ctgctcacga agaaaacgat ggacaatttg cagaaattga
    29641 tttttctgga gttcttccgg taattctttt tcatattttt gcagcatctt ctccggcgtc
    29701 atgacacgac caccgatacg ggtcagcatg gttttaagag aatttgaatg atgtaaatgg
    29761 tacaaaacat caatcacgtc gtttctttga ccaatctcat taggagtcag caagcgacct
    29821 tccagccact cttgtgctgt caaagtatcg ccattccccg ttcgttttgt ccaaacaagt
    29881 tttggtgtaa ttccttccct tgaaagagca gccaacatcg gagtcgtgtt tcgtttaata
    29941 aagactttgt cctcatcacg catgcctatg tatgtttttc ctgtgtcgcc tttgatggga
    30001 cgtaaccgcc actcttggtc taactgaaac tccatcatgc attctcccgt cctcttactc
    30061 tttgttttgc agtacttatt attctagtcg cctgaacact acacgtcaag cacatatacg
    30121 ttctttttca tttaattgcc attcaaaaaa ccagcttttc tcaaaaaaaa tagaacgaac
    30181 agcattctta ctgtcaacgc tctatttttc attctcattt ttaccctaca cagctttgtt
    30241 ttcagtctga catcttcttc aaacgagttg gtacatatag tttaacaaag agcatagtaa
    30301 caatcaaata agccgctagc ggaacccatc gctgctgccc atctaatacg actgcagcaa
    30361 tcaaaccaaa aataaccgac gcgataatta atacccatgt gagtagatac tgaatcgcct
    30421 gcttcttctg attcttatgg attggataaa gctgaacgag aaccatatag cggaactggt
    30481 tcgctaaagg taacagttgg aaaccgatca aatataaaaa taagaaaccg ataatcagac
    30541 taaaataaaa gtcgactact ccggcaatca ataacccacc tataattact aatcggaaat
    30601 aaagaccact aaaatcagaa cctcttgcca atcttcttgc aaacaagtaa agataagtgt
    30661 tttttgattc tcttttaata tgagtcaata taggatccaa atagcgtctt cttttgatct
    30721 ttgccgtaat ttcaggaaca tctgtgaata aattgataaa ttgatagatc tgatgcaacc
    30781 tatgttcttc tacatgcacc attttctccc aatccaatcc tttttcttgt tgcttccaca
    30841 aaaaccagta atatagactt gcctgtacta atgcacctgc tattcctgcc catattagcc
    30901 aataaaatga tagaaacaaa aagatcagag aagaaaaaaa ccagcctaat ttccaataac
    30961 gaataacttg ttgatctagt cgaaacaatt ctgctctctt gatttgcaga tgactgaatt
    31021 tcaaactcca taatatgatc agatagacga aaaacatcga aaaggattgc tccgtactaa
    31081 ccaccactaa tggcatggcc aatccgcatg ccaatatcaa aaagatcaac ggaaaaatgc
    31141 aagaatagcg aaaagctcgt tccaaatagg tttgcatttc tttttcttta ggaagtaaaa
    31201 aaacaatatc tgctgcctct gcaaaagtag ccatcttacc cacgaataaa cagagcatcc
    31261 aaaatacaga gattatgagc cctccaattg caaacggtgg ctgcagggtt ttcaaccaat
    31321 tagaataata taatcccaca ccgccaatca agaaaagaca aactaaaaca aaatgatcgt
    31381 tcaatacata acgcatatat ttcatcattt tcttttgatg tcttgctaaa cgtttttgaa
    31441 aaaaactact catgtgcctg ctcctttgtt aaagcaatat agatttcgtc caaagaagaa
    31501 tccggcagct gaaattcttt tctcaacatc tcaagagaac cgtctgctcg tatctctccg
    31561 ttatgtaaga ccacaaaacg atcacaatat ttttctgcag tagctaaaat atgcgtagac
    31621 atcaaaatac cggctccttc ttttttcatt tctaccatca attcaagcaa tgcattgata
    31681 gctagtggat ccaagcctaa aaatggttcg tcgatgatat ataaagaagg ctcgatcaaa
    31741 aaggcgcata agatcatcac tttctgtttc attccctttg aaaaattagc tgggaaccag
    31801 tcaagacgat tagataaccg aaaagtctcc aacaactttt ctgctctttt cattgctata
    31861 tccataggta tatcgtaggc catcgcagtg acttcgatat gttctctcaa tgtcaattct
    31921 tcatataaag aaggagtctc tggaatatac ccaatttgtt tgcgatattc ttccgtattt
    31981 ttctttaagg atagatgatt gatttctatc gttccttttg aagcttccag cagaccgata
    32041 atgtgcttga tggtcgtact tttccctgct ccgttcagtc caatcaaccc tactatctct
    32101 ccaggctgta catcgaaact gatatctttt aacacaggga gatgtccgta cccccctgtg
    32161 acatgatcga taattaaact cattcttttt cctccgccat ttatttttat ttattatacc
    32221 acgaagaaaa tgacggtatt ttgttttttt ccttacaata gctttaactt atggtaaagt
    32281 taagctgaag tatttattct ttatagaaag gatgttttat atggaaaact gtattttttg
    32341 caaaatcatc aatcaagaaa tcccaagcta taaaatatat gaagacgaca aggtctatgc
    32401 ttttttagac atctctcaag caacgaaagg acacacatta gttgtgccta aacgtcatgt
    32461 agcagatatt tttgaatatg atcccgaact tgcaggagag gttttttcgc gtgtgcctaa
    32521 gattgctcaa gcattggaaa aagcttttcc agaaatgaaa ggtcttaata tcttgaataa
    32581 caaccgtgaa ctagcctatc aatctgtttt tcattcacat atccatttag tccctcgcta
    32641 cagcaaagag gatgattttt ctattcactt tgtcaatcat caagatagtt atggatcgga
    32701 agaactaaaa gctattcaag aaacaatcgt aaagcaggtg tcctgcgatg attaaaaact
    32761 ttttcaaagg actgttattt ggagctgctg ctggcactgt cggaggtctg ttattggcgc
    32821 ccaggagcgg gaatgaaaca agaaagaaac tgatcgaaga attggaagaa gcaacaactc
    32881 tgacaaatga tttaaataac agcctgaatc attttaaaag cgcattgatc gaaacaaaag
    32941 aaacagctga atctgtgatt cctgctttcc aagaagcaat caaaaaagat atcgaagatt
    33001 ttaaattcca agccgaacca cgtatcacac aaatcaatga acaagtagaa aaactaaaag
    33061 ctgatctgcc agagtatcct gaacaaatat aataatgatt attttctcaa cagatttaac
    33121 tatataagtg agaaaagttt gcgtatttcc taggaatgtg atgaaatttt gaattttgtt
    33181 tgaaactacc acgacaacgt ttttaaagga aatgggatat ggtatagtta atatataaaa
    33241 ataataacta attacaggag tgcaaatttt aatgaaaaaa aaatcaatta ttttggcagc
    33301 tactagtgcg ttggctgttt taactctagc agcttgttca ggcgatacta ataaagatat
    33361 cgcaacgatg aaaggcggaa cgatcactgt atctgatttc tatgatgaag caaaacttga
    33421 atcatcgaac caatccttag tacaacggat gatcatttat aaagtattca ataacaaata
    33481 tggcgataaa gtaaccgata agcaagtgga tgcagaatac gacaaacaag cgaaatcatt
    33541 aggtgatact tttgaatcac aattagaagc tgctggatat acgaaagata cttataaaga
    33601 atatattcgt aacaatttag cttttgaagc tggcttgaaa gctcacgtag atattacaga
    33661 tgacgatttg aaaactgctt ggaaatcttt ccatccagaa gtagaagcac aaatcatcaa
    33721 attatcaagt gaagatgaag caaaagatgt gaaaaaatca gcggatgatg gagatgattt
    33781 ctctaaatta gccaaagaca aatcaactga tacagagaca aaagaagatg gcggtaaagt
    33841 taaattcgac tcaacgacaa caactattcc tgcagaagta aaagaagcag ctttcaaatt
    33901 gaaagacggt gagatctccg atgttattac tacaacaaat cctacttctt atgcgacaga
    33961 atactacgtt gtcaaaatgg tgaaaaacca aaataaaggc aatgacatgg ataaatacaa
    34021 agatcaacta aaagatatcg ctactgaaac taaactaagt gacaatgcat tcacaactaa
    34081 agtaatcggt gaagaattaa aagatgcaaa tgtaaaaatc aaagatgatg cctttgaaaa
    34141 tgtgttgagt gcattcacaa caacttcatc aagtacgaaa gactcaagtg aaactacagc
    34201 ttcaacaaag agttctgaca caaaatcaac tgattcaaca aaagaatctt caactgaaga
    34261 aacaactgat tcatcaaaat aaaagaaaac aaaaaaagcg aagacttatg tctcgctttt
    34321 tttgttttga atgaaaaaaa taaatttttg ctataaatct tttggtacat agaaacttcg
    34381 gttgtccata ccgaaaatcc gatcggtgta ttttcctggt tctgtttgac gaatcgaagt
    34441 caacatcatc tgcatcgaag catcgatatt gtcgatttga tgtaatattt ctgcttccat
    34501 tattcttgga cgtacaggtg agccatattc taataagcca tggtgtgcta aaaccatatg
    34561 acgtaatacg agaacatctt cgtcagcttc gctgattttc aacgcaagac aggctttagt
    34621 tatttcttcg tcaatcaata ccagatgacc gatcaaattt cctgcaagcg tatattctgt
    34681 cgacatagga ccggaaagtt caatcacctt tcctaaatca tgcaaaatga taccagcata
    34741 caacaatgct cgattcagct caggatattc atcggcgatt gatttcccta agtgaagcat
    34801 tgtcaaagta tgataagcta atccactggc aaatgcatga tggtttcttt ttgctgccgg
    34861 aaactcaaaa aattgttttt gatatttatt aagtagatag cgaacaatac gattccaatg
    34921 cgcattggtg atctcaaaca acaattgatt gatttcttct tccatttctt ctttcttcaa
    34981 aggggctctt tccatgtata aactaggctc tcctggctca tctacccttg ttaagcgcat
    35041 atgaagaatc tttacttgag ggtttccttg ataaacctct cttttaccgt ttagaaaaac
    35101 gactgtgccg gctgtaaatt tattgatttc ctcctccgaa gcatcccagt atttaccatc
    35161 gatagtacct gaagtatctt ggaaagtaaa agcaataaat ttttttccgt ttttagcgat
    35221 tcttacatct gcacttttga tcaggacaaa ttgttcaaac tgctcatcga ttactaaatc
    35281 acgaattttt ttcattattt gctccttcta attgttttac gatttggttc atctcatggt
    35341 aatagcttac catttcttta tcagaagaaa aacaaattat ttgatacttt tctccaaatt
    35401 tagctaaaag ttcagctaat tgatactttc gctgatgatc ataatgaagc cagccatcat
    35461 caatgatgat cgggcatatc gaacggttag cttctaatga gaggtaagca aatctcatcg
    35521 ccataatcag ctgatctttc gtacctgtag ataactcata gatgggaaat tctgtatatc
    35581 cgtctgttaa agtcagtgta tcatttttca gtaatatctt ctgataattt ccttttgtta
    35641 aaatacctaa ataatcacct gctgctctta acaactgggg taattgtctt tcactcatct
    35701 ctgttgaaag atcacctaag aatgtcccgg cgatttcaag tgctccccat tcctccagta
    35761 attcctgtaa ttttgctttt tgccggctag cttgttgata aagttcgtcc aatgtgccgt
    35821 ctctttgcaa ctcttctact tgaagttgga gtcgttgccg attttcactt gcttcttttt
    35881 cttcttgctg gagttgggat tgcttttctt tcagttggcg cagtcttaca gataatttct
    35941 cttttgtcag ctcttcagga aatagtggag taagcatttc cgataattca ttgtatcttt
    36001 tcatctgctg ttcggcttgt ttggcttttt gtaaaaagac aggtacttcc gaaggataag
    36061 ataagcctgc ttgagtcaat tgttcgctgt attcttgtat ccatactttt tgtttctttt
    36121 tcaattgatt gatttcttgc ttcaataaag tattttgctg atagcttctg gcaaatttca
    36181 tctgttccat ctcttgtata aaacgggaaa gcaataccat tttttctgaa attgtcttat
    36241 cttgtatcgg taaccattct ttaacaaact caaatttttg gtcgatttgt cgtagttttt
    36301 cttgtatttc ctgttctctt ttatgtttcc gctcaaatac gccatataat ttctgataat
    36361 ctatgacttc ctgtccatat tctttcatga gtttgagcgt atccatcttt cctaagtgcc
    36421 gatgatccgc aaaacgatat acttcttgct gaagaatctg tttttcttct ccagttctct
    36481 tttgtttttc ttctgcttca gctaattgag ctaaggctag atcaagttgt cctagttttt
    36541 cttgccacat tccttttatc tcagagaaat catttttagt tttaattttt ggttgccttt
    36601 tgtagaagaa atagccaatc atgcctgcac caattagtaa aaggatcact ttcaagggca
    36661 ttggtacaaa tatacctgca atgattattg ctccgccagc catcagcata tacggtttag
    36721 atgaagagac tggtgctatc tctgactgat tttgtaataa tgctggatag gtctcttcca
    36781 agtcattcac ttctgcttca atcatctctt gctgttcttt agctatttgg agacgcacag
    36841 aaatctggct ttctagatca gcaatccttc tttcttcagc cagaagatgc ttccattctt
    36901 cttcatataa aaattcttcc ggtaacggat ttttctgcca atgccacctt tgttccaaaa
    36961 tcttcaattc tgaatttgtt tctctagtct caatttgtaa tctttgccat tcttcgttta
    37021 attgttccgc tgtaaattga agttgctgga gatcttttag ttcctgctcc tgctctaaat
    37081 aaaaataata tctggctgac tgctgatcca tcccgctatg cttttccagt gttgcttcta
    37141 attcttccaa acgctcattt gtttgctgat accgtttgga gaaagactcc agatctaaaa
    37201 gaagttgctc atcaggaaga cctgtagtgc tgtttttttt gagagattgc cattcttcat
    37261 ataaagaaaa attaagttct tgctgtctgg ctaatgaaaa ttgtttgttc gcctctttta
    37321 attgtgccaa taactgctgc tgttgcgact cgtattcctg ttcttgttgg atcaacttgc
    37381 caaaataagc ttcctgttct tgtttttgat gaattttttc ttgcagctct ttccattcat
    37441 ttaatttttg attgattagt aatttttggc cttttttctt gaataattgt tgtgctttct
    37501 gataatattc ttgccgcttt tggaatactt gagaactgcc cgtgatgcct aaagaaatca
    37561 gcgcatcatg tagctctttt tctctcaaat gatctagttc catcaactgc tcttgctgga
    37621 aagtaaatac ctgctggaat aactctttcg tcaaaggtgc gatcagtttt tctagtaact
    37681 catcacttcc cacttgatcc cccagccaca ccttcgattt tccgcgattt tgctgcttgt
    37741 agcgctcgac agcaaattca ccatatacag gatgtttaag ccatattttg ccgccatagg
    37801 ctgagccgtc ttttggtgta taatcctttt ttttacggcc ttttgaaggg aaaccaaata
    37861 atattgcttg aataaattga taaatcgttg attttcctgc ttcgtttccc ccatagatca
    37921 gctgattgcc tgaataaaag tcaaaacttt tttgacgcca ttttccaaaa gcagcaatct
    37981 cgatccttaa aatcttcatt cctgatcctc ctcaaataaa aaatcttgat taagcagctg
    38041 cttggctttt tcaagtgttt cttcttgata ttccggcagt tcgctcaata ttctagccgc
    38101 ttcttgatga ctatatactt catttaaaat ttgttggaaa tcttgaggtg tctgatagta
    38161 ttgaaataac tgttccatca atttagcaga agcagcaagt ggggtcttcg tataaaaagt
    38221 attctctatg agagaaatac gccaaataaa gaaatcattt atcctatctg agaattcctc
    38281 taacaaatag tctaatagct ctcctgattg tatccgttcc aaaacatctg tattgagatg
    38341 gtcataatct tttaatttta tctcaattaa actaaatcca tcctctactt ttgaaagctg
    38401 ctgacgaatg tgaacaatga cctcttttgt cgttcttgct ttcctcaatg atatttcctt
    38461 tgtcttccaa tagacctctg ctactttaat cggatgtatt tggcaacttc ctttacttaa
    38521 ttcaactagt agaatagatg tagaagcttg ttccttttta gtatgacctt gaggcgcacc
    38581 tggataaaca attgtttgct tttcatttaa gaccgttggc acatggatat gccctaaagc
    38641 ccaataatcg tatccctttt cttgcatctt tgagggttgg aaaggagcat agtttccttt
    38701 ttgagagacg ccaggttccc cgtggtacaa cccaatatgg taatctgtga gttctctaga
    38761 aggaaattcc atcactttgt ctttttgaat ccacggttga cgataactaa atccactgag
    38821 agagacagtt tctccacttt ttatcgttat tttttttgtc tctaccgttt cggatgtaaa
    38881 taaatggaca tttttaggaa aggagaacca ataccgctct ttttgataga aatcatgatt
    38941 tccgaatatt ataaatacag gtatatggtt cttttctaac cgtttcatct gttctgaaaa
    39001 atgtttctgt atttttaatg aaggacgatt ttgatgaaaa ttatctcctg caagtaaaac
    39061 aaaatcaacg ttatttatta ttgcctgttc aatgatattt gataaaacgg tcagattcgt
    39121 cttcaatagt ttttcctgta cagttttatc aagagttgtc agtccttcaa atgaccgatc
    39181 catatgtaaa tctgccgcat ggataaattt caccattaaa aatttcctcc tttttttctt
    39241 ttatcatacc attttcaatg ttttttttca tcaataaccg aaaatttgtt cgtattatgc
    39301 aaagaaatta cgataaatgg ccgacttcaa aaaataagtt tattcgaaaa aaagattgtg
    39361 acatgatttt tgtcacaatc ctaaatatct atgttatatt cgcttcgttt gacactgaag
    39421 aagtactatt tgtactggaa tttatgctcc atttttccgt atttagaatt ctatgttcta
    39481 tactacctta attagtttat atggtaagaa ttctttcaaa tgcttgagat actccgtctt
    39541 tatcatttga aacagtgaca taatcagctt ttttcatgat ttcttgatga ctatttccca
    39601 ttgcaaaact aatatctgca ctttcaaaca tagataagtc atttaaacta tctccgatcg
    39661 cgattctcaa aggtttcttt aaattgtaga ttgattccaa acgatcaaac accacacctt
    39721 tagatacatt ttgaggtacg atttcaatat ttacaggtgc cgattcagta acagacacat
    39781 ttagcatctt tttcaaatgg ttaaatatat tagctttcaa ttgacgatta ggaataaaaa
    39841 taaaaaattt gtaaatgctg atagcatcct tttctataat ttcctcaata ttattataag
    39901 ggattgaaca aatagatttt tcatactcaa gctcatagtt tagctctttg atagaaaatt
    39961 cactccctac ctcgtgaaaa atttttttca ttttattttt ataattctga gattcataac
    40021 ttccgagatt tgtatatatt ttggttggaa atttcgatag gacagaatca atttttttta
    40081 catcattttt atcgaaggaa gactcaaata tctttttttc tccatcaaat ccaacagaac
    40141 catttaaccc tactgctggt atccttaaac cagatttttt gagaatatta ttaatgtctt
    40201 ctacttctct tccagaacaa atgaatggaa aacagtcttc cggtaaatgt ttcattgttc
    40261 ttgcattttt ttgactaatg ctctggtcag aattcaaaag agtaccgtct aaatctatac
    40321 caattagttt catttcatcc atgtctccta tacgttgatt ataaattcac tgcttattgt
    40381 aagtctaaat ttctcacaaa acatatacta attataccta atttaagtaa gtacaaacat
    40441 cattttaaga ataggaaagg acatttatat actattctct gtatttttga tttataaata
    40501 aatatgaatt gaatccaaat cagctatatt actattttat aataaccaac tataaaaaac
    40561 gaagttaggt gaaaaactaa cttcgatatt cataaaagac tctattagtt caacttagca
    40621 aaagatttaa a
//
LOCUS       DACEOM010000017        40620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEOM010000017 DACEOM010000000
VERSION     DACEOM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.018"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            336..1439
                     /locus_tag="IW781_000877"
     CDS             336..1439
                     /locus_tag="IW781_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1181892.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="IW781_000878"
     CDS             1723..2403
                     /locus_tag="IW781_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1181893.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="IW781_000879"
     CDS             2493..3527
                     /locus_tag="IW781_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1181894.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="IW781_000880"
     CDS             3541..3876
                     /locus_tag="IW781_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181895.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="IW781_000881"
     CDS             3860..6340
                     /locus_tag="IW781_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181896.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="IW781_000882"
     CDS             6396..7955
                     /locus_tag="IW781_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1181897.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="IW781_000883"
     CDS             7952..9316
                     /locus_tag="IW781_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1181898.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="IW781_000884"
     CDS             9487..10557
                     /locus_tag="IW781_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181899.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="IW781_000885"
     CDS             10572..11240
                     /locus_tag="IW781_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181900.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="IW781_000886"
     CDS             11260..11838
                     /locus_tag="IW781_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1181901.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="IW781_000887"
     CDS             12241..13152
                     /locus_tag="IW781_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1181902.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="IW781_000888"
     CDS             complement(13408..14325)
                     /locus_tag="IW781_000888"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1181903.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="IW781_000889"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="IW781_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1181904.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15570
                     /locus_tag="IW781_000890"
     CDS             15022..15570
                     /locus_tag="IW781_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1181905.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15685..18045
                     /locus_tag="IW781_000891"
     CDS             15685..18045
                     /locus_tag="IW781_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1181906.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18132..18446
                     /gene="trxA"
                     /locus_tag="IW781_000892"
     CDS             18132..18446
                     /gene="trxA"
                     /locus_tag="IW781_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1181907.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18628..20406
                     /gene="uvrC"
                     /locus_tag="IW781_000893"
     CDS             18628..20406
                     /gene="uvrC"
                     /locus_tag="IW781_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1181908.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20551..21450
                     /locus_tag="IW781_000894"
     CDS             20551..21450
                     /locus_tag="IW781_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1181909.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21443..22705
                     /locus_tag="IW781_000895"
     CDS             21443..22705
                     /locus_tag="IW781_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181910.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22868..24766
                     /locus_tag="IW781_000896"
     CDS             22868..24766
                     /locus_tag="IW781_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1181911.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25065..25784)
                     /locus_tag="IW781_000897"
     CDS             complement(25065..25784)
                     /locus_tag="IW781_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1181912.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26121..26453
                     /locus_tag="IW781_000898"
     CDS             26121..26453
                     /locus_tag="IW781_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181913.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26440..28443
                     /locus_tag="IW781_000899"
     CDS             26440..28443
                     /locus_tag="IW781_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1181914.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28462..28932
                     /locus_tag="IW781_000900"
     CDS             28462..28932
                     /locus_tag="IW781_000900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1181915.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28953..29537
                     /locus_tag="IW781_000901"
     CDS             28953..29537
                     /locus_tag="IW781_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1181916.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29552..30538
                     /locus_tag="IW781_000902"
     CDS             29552..30538
                     /locus_tag="IW781_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1181917.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30528..30839
                     /locus_tag="IW781_000903"
     CDS             30528..30839
                     /locus_tag="IW781_000903"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1181918.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30861..32642
                     /locus_tag="IW781_000904"
     CDS             30861..32642
                     /locus_tag="IW781_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1181919.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32635..34011
                     /locus_tag="IW781_000905"
     CDS             32635..34011
                     /locus_tag="IW781_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1181920.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34018..34653
                     /locus_tag="IW781_000906"
     CDS             34018..34653
                     /locus_tag="IW781_000906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1181921.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34683..36038
                     /locus_tag="IW781_000907"
     CDS             34683..36038
                     /locus_tag="IW781_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1181922.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36230..37624)
                     /locus_tag="IW781_000908"
     CDS             complement(36230..37624)
                     /locus_tag="IW781_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1181923.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37716..38687
                     /locus_tag="IW781_000909"
     CDS             37716..38687
                     /locus_tag="IW781_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1181924.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38684..39466
                     /locus_tag="IW781_000910"
     CDS             38684..39466
                     /locus_tag="IW781_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1181925.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39635..40006)
                     /locus_tag="IW781_000911"
     CDS             complement(39635..40006)
                     /locus_tag="IW781_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1181926.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40140..40583
                     /locus_tag="IW781_000912"
     CDS             40140..40583
                     /locus_tag="IW781_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1181927.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
BASE COUNT        13420 a         6917 c         8650 g        11633 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    15481 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    15541 gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat atttcgtttt
    15601 ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca gctctttttt
    15661 catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa
    15721 aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga agaactggca
    15781 gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga aactgaggat
    15841 ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaacttga aaatatccgt
    15901 ccccatatga aacggatcga gatcggagca gacttgaatg gagtggagct tgcacaagtt
    15961 tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct ttctgacagt
    16021 gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat ccctgttttg
    16081 agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga cgcttctcct
    16141 gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg tgaacagtta
    16201 gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat caccatgcga
    16261 aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc
    16321 cacgatcaaa gtgcttcagg acagacttta tttattgaac caaaacaagt ggtggatttg
    16381 aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg tattttaagt
    16441 gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatgc ttatgtgatt
    16501 ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa agccatcgtg
    16561 ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc actgatcgat
    16621 caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc catcgtcatt
    16681 acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct gttacaactg
    16741 atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag
    16801 gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc gaccttttct
    16861 tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag tctggtatta
    16921 tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc aatcgcaatc
    16981 ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta tcctgaattg
    17041 aaagtttatg gatacaatcg ggcaaatacg attaatgcca gcatggagtt cgatgtggat
    17101 acactaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa
    17161 atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca gttgatgaat
    17221 gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg gaaaatggca
    17281 gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact tcacgatgat
    17341 ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag
    17401 aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat
    17461 attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca gttgatcgat
    17521 gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa taaagtattg
    17581 aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt tacgacttac
    17641 ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga aatcggtata
    17701 ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa agagccgaca
    17761 caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa tcaattggac
    17821 cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt ggattctgcc
    17881 attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg cgcacttcgc
    17941 aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga gtttgcaccg
    18001 gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt actaaaggta
    18061 agcagatggt tcgctatcca agtatagaaa tgctatacta atcatagatt ttaaaaatgg
    18121 aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa acagataaag
    18181 gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga
    18241 ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct aaaatggatg
    18301 tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca acattaatgt
    18361 tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac
    18421 gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga gggtgggact
    18481 gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa attaatattt
    18541 tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc tataataact
    18601 ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa attagcttta
    18661 cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat catttatgtc
    18721 ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggcag ccataatacg
    18781 aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac agaatcgaat
    18841 atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa atacaacatt
    18901 atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa atatccccgt
    18961 ttggtgatta ctcgtaaagt actaaaagac aaagcactgt attttggacc atatcccgat
    19021 gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct gagaaaatgc
    19081 aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg tctgtgtcct
    19141 tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt gaaaaagttt
    19201 ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga aaaagctgct
    19261 gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc cattgagaca
    19321 atcatgactc gccaaaaaat gacaaatact gatttactgg atcgagatgt atttggttat
    19381 gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt
    19441 gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt cttgacttac
    19501 ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt gattcctgat
    19561 acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca accaaaacga
    19621 ggagaaaaga aaaaattagt tgaattggcg aataagaatg cctcagttgc tttgaatgaa
    19681 cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga acgtttagga
    19741 aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc taatatcatg
    19801 gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa
    19861 tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc aatgagagaa
    19921 gtcatctatc gcagatattc acgagtgatt cgcgaagggt tgcctttacc tgatttgatc
    19981 ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga aaatcaatta
    20041 ggaatcgata ttcctattgc tggcatggca aaaaacgata aacataaaac aaatgagttg
    20101 ctattcggta tagatttggc tgtagtaccg cttgagcgaa attcaccaga gtttttctta
    20161 ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg gcagctacgc
    20221 agtaaaaaca gttttgcttc ccgattagat gaaattgaag ggttaggacc aaaacgaaaa
    20281 aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac gacagaagaa
    20341 ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa attacatcag
    20401 gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta tgtacaacaa
    20461 aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg tctaaggata
    20521 tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct tgttaaaact
    20581 tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg
    20641 gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat cttggatttt
    20701 ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga gaaagattat
    20761 gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac gattcaagac
    20821 caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga accaaaaatc
    20881 gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg
    20941 tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga acctaaattg
    21001 atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt gctaaaagat
    21061 ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca caatatggat
    21121 aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat ggttttgaat
    21181 ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt ggaatctggt
    21241 ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac
    21301 aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt tactcgtgcg
    21361 actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga agagattttc
    21421 aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag atgtctattt
    21481 aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta tcatgctggg
    21541 tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata aaatcggcgt
    21601 attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag atgattttga
    21661 gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga
    21721 tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt atagcgaaaa
    21781 ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct tgcaatcgat
    21841 gcttcgagca agtcaattag gcatttcacc agaagaagta gctagcttga gccaacaagc
    21901 aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag ttggagaaga
    21961 taactcgaca gtccaatatg tcgtaagtta tgtagcaacg attgtcttgt tcattattat
    22021 tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat
    22081 ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaac taacaggtgt
    22141 tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta taggatacag
    22201 tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat tggataactt
    22261 gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt tgattttttc
    22321 tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag caaaagcaat
    22381 tttgccagtg acttatctat ctctaggcgg atatatgctt ggattgatac taggagcttc
    22441 agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat cttcatatat
    22501 tatgccggtt cgcttagcaa atgagacagt cggtatgggt ggagcattga tttccttgtt
    22561 ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt acaaatcgaa
    22621 tgtactcgtg tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat
    22681 gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat agaaggaagc tggacattta
    22741 ttgttcccag cttctttttt gtatagacct atttctattt ttttattata ttggatatat
    22801 atattcattg aaaatagagc ataacaactt tataatgttg agtataccaa tttttggagg
    22861 cgttttaatg gaccgctatt tagatttact ttcagagaag ttccctacaa cggaagatgt
    22921 catcacggaa atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt
    22981 tttgagcgat atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg
    23041 aaatcttaaa gaaaagatca aagacctttt ctctgaacaa atgacggaaa cggagatcag
    23101 ccaattcact atttttacgg cttatccaga atatgtgttg acgactgatt ggtacaaaaa
    23161 acaagataaa tcccagctga tccatcgttt gatcgatctt ttacggttta cgacagtcaa
    23221 atacactcga tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga
    23281 actcttgtat atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga
    23341 acaacttgtc ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc agacgatcca
    23401 aaaatctgtc atcgatcatt tgcatatcgt gggtgatatt tttgatcgag gaactcaagc
    23461 agccaaagta atggatcgaa tcatggattt ttcctctgta gatatccagt gggggaatca
    23521 tgatattttg tggattggtg cgtattgcgg ctctgaggca tgtttattga cgcttttacg
    23581 gattgccgca cgctacaact atttatttga attagagaaa gaatatggat taaatttacg
    23641 tccattattt tcttttgccc atcaaaccta tcagaagaat ccagtattta cccctaaaaa
    23701 caggaaagat ctatctgaaa gagagcaaga agaattagaa aagatccatc aagcactttc
    23761 tatcctccaa ttcaaatcag aacatcagtt gttgaatcga cgacctgaat ttaaaatgga
    23821 cgatcgtaaa ttgttggaaa aaatcaacta tgaggaatca acgatcgttt tagaaggtca
    23881 gctctatgag ttgaaaggga cttgttttca aacgatccag ccagatgatc ctaaaaaatt
    23941 cttggaagag gaacaaaaag tgatcgatag tttgatgtat tcattccagc actcgttaag
    24001 aatgcaaaaa cacatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca
    24061 gcatgtactg ttccatggct gtatacctgt agatgaaaag gggcagctct tagcatttga
    24121 attagaccaa tcttatcgcg gaaaagaact gctgggattt tttgaaaaac agataactga
    24181 aagcgcaaaa gatttgactg caaatgaaga tttggcgacc gatttgattt ggtatgcgtg
    24241 gagcgggcca ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg agcgctattt
    24301 tctcacagag cctcatacgc acgtcgaaaa aagcaatccc tattatcact tgcgagatga
    24361 agagtggttc agtgaaaaaa ttttggcaga attcgatgca aaagaagaag gttcagcaat
    24421 catcaatgga cacacgccag taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg
    24481 aaaagcattt gttattgacg gaggtctatc caaagcttat caaaaaacaa ccgggattgc
    24541 tggctactca ttactatgca attcttacgg tttccaaatc gtgacacatt atccgttctt
    24601 gcctattgaa caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga
    24661 gcagcagctc ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca
    24721 gattactcaa ctccaaaggc tgttggatta tcgaaaggac gattgagaaa aatgctagcc
    24781 aacattgctc aatagactaa aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg
    24841 aattagctca aaaaacggta aaatattaga agctattttt ctgtttattt ctctaatact
    24901 caggtcaggg cgctgatttc acgacctctt ccttaaacgg tgttctcctg ccaactcctc
    24961 ggtcacaagt cacaatatcc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg
    25021 actcagagtt gaacggcagg ctcacgacct aatcagaaaa acgattaaaa ttcattcaca
    25081 aagaaatcga ataaacgctg ttcagatgaa tctacacgat gagaaagttc cggatgccat
    25141 tggacaccaa ggattttgtg catattatct ttactttgga ctgcttcaat gagtccatcg
    25201 ttggaaaagg cgatggcttt taacaaagga gaaagatctt tgactgcctg atgatgatag
    25261 gaattcacaa agtatgaatc agggagaagg tcacttaata aactgtccga aacgattttg
    25321 acttcgtgag ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac ggaccatttt
    25381 ggatataatg aaagatcttg ataaagtgtt ccctccaaca cgacattcaa tagttgaagt
    25441 cctcggcaga ctgcaaaaat cggttttctt tgtttcaatg cttccaagat caatgcctgt
    25501 tcgaattggt ctcgttgaat atttgtttcc gtcagttttg gatgagggtc ttccatgtaa
    25561 agttgcggac taacatcctg accgcctgca agaatgagtt tgtcgatttt ctcaattagt
    25621 tgaggcgctg aatctggaga catgactggc agaataagag gtaatccttg agcatctaaa
    25681 actgcatcca caaatccctg cggtgtatag cttacttgat ttccttggaa agtatctgtc
    25741 gctcgaatca gttgattccc ggcaatgccg atgattggtt tcatttttat tccccctaaa
    25801 gtagactaat tgtaacaagg atttaaaaaa atttaaacta tgtaagactt gcccctatac
    25861 ttatctgttc ttgctgtgtt actatctgta aagagaattt tcgtgtgaaa ttcatgacga
    25921 tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg
    25981 cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct
    26041 gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct
    26101 cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag
    26161 cagaagaaga aaatgagaag cgtgctcgaa ccctacggca gcagttgaag acctatgcag
    26221 aagaaaaaga gcaagaattg gagataatcc aaacgaacag tcggcaacag ctgaaacagt
    26281 tggtagaaga aaaagaacaa ttcgaaaagc aagaattaga aaaagaaaaa atgaaattga
    26341 aagcagaagc agacaaggtg caagcaactt tgaaagacca atatcaaaag catcagagtc
    26401 aagcaatcgg tgcaatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa
    26461 aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga
    26521 atccaaaatg ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtagaa
    26581 aaatattttc cagataccac gattattgat aaagaagcaa aattaagcgc tttaggcaat
    26641 cggctgaaag acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa
    26701 aagaaactcc atttgaaacg tcaagaactt tccttacaag acttggaagc aaattattcc
    26761 gaagaaaatt tcatgaataa attagaagaa gtccttgatt taaaagaaaa gtgggaacag
    26821 ctaactgaaa aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag
    26881 ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg
    26941 agtgcagcaa attttgaaag tttcaaggaa gaactaagta agcttgatga cgtttatatg
    27001 gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca
    27061 gttgctgcta tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat
    27121 gaacaaaatc ctaaagaaaa attgcaagaa accaaacagc aattatccat tgttcacgag
    27181 cagcaaaaaa agataacgac agcgttaggt cagtgcagcg gctatatcca agaatttgaa
    27241 tgggtagaag aagtcacact tgccgttgca gaacgggaaa aagtaaaaga acggtttatc
    27301 catgccactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg
    27361 atgcatgtgt taaacgaaac tgtatcagaa gaagatatat acgttagttt cgaaacacca
    27421 aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc gcttatcgaa
    27481 ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaacg
    27541 ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttac
    27601 ggattgctga tgctcattgg gacaatacta gctcaaaaac tatttgtttt accccgcggg
    27661 atgaaacgtt tcgtaaaatt ctttgagatt ctctcggtac catcgatcat ctggggattg
    27721 atctacagtt catttttcgg acaggcatta ccaaaagaac tatttggtat ccgcttgcct
    27781 ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc
    27841 ggtctgatac agatcttggt tggattattc gtggcagcaa aggaaaatat caggaggaaa
    27901 gattacttga gtgctgtcag tgatggtttt gcctggcaag ggatcctgat tgggatcgtt
    27961 attgcattga ttggtgtcat gttgataaaa aatacagcat ttgtttatct tggcgcgggg
    28021 ctatcgatca ttgctgcttt atgtatctta atcgtgccga ttatccaatc gccgtctaaa
    28081 gtcaagggtg ctgccaaagg agcgtataat ctttacggat tgactggtta tatcggtgat
    28141 ttagtcagtt atacacgttt gatggcacta ggtatttcag gcggaagtat tggcgcggca
    28201 ttcaatatgc tagttgcttt catgccgcca gccgctcgat tcagtgtagg gatccttttg
    28261 atcatcgctt tacacgcact gaatatgttt ttgacactgt taagtgccta tgtccatggt
    28321 gcacgattgc agtatgtcga attttttgga aaattttata caggaggcgg acgtgccttt
    28381 gacccgctga aaacggcgga aaaatacgtc aacatcaatc ataagaaaaa aaacaataaa
    28441 taattggagg atttttatac aatgatggat tacttaatta gtcaaaatgg tggaatggta
    28501 tttgcagtat tagcgatggc aacagcgacg attttttcag ggattggttc agctaaaggt
    28561 gttggaatga caggtgaggc agcagcagcg ttaacaacaa gtcaacccga aaaattcgga
    28621 caggctttga ttctgcaatt gcttccaggt acgcaagggt tgtatggatt cgttatcgcc
    28681 tttttgattt ttatcaactt aggaaatgat atgtcagttg tacaaggatt gaactttcta
    28741 ggcgcttctc tgccgattgc ttttacaggt ttgttttcag gtatcgccca agggaaagtt
    28801 gcagcagcag gtatccaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt
    28861 tttgctgcga tggttgaaac atatgcgatt ttaggtttcg ttatttcctt cctattagta
    28921 ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc
    28981 agatgaatga agcagcagag caagaaagag cggcgttaga gcaagaagag cgaatgaaga
    29041 tcgatcaaaa ctttgaacaa aaacgtacgc aaatcgaaac agaacatcaa aaacaaaaag
    29101 aaaaacaaat cgaattactt gaaaaaaaat accgtcagct taggaatcgc caacaagtag
    29161 aagtacgaca agaaaatttg aatgcgaagc aggaattttt acgacgattg tttgcagatg
    29221 caatagctga aatggagaat tgggatgaat ctgaacaaat ccagtttatc aaaaattcat
    29281 tgtattcatt acctttgaca ggcaaagtaa cattcattgc tggagaaaaa tcagcagctt
    29341 atctttctca gacattgctg gatgaatgga acaatgaact tccctttatg atggttttaa
    29401 gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata
    29461 attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca
    29521 atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat
    29581 tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa
    29641 ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga
    29701 cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg
    29761 gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gctatacttt
    29821 ccataatctg aaggttttga caaaagccga gatgacaaat caaaatctgg accatttgta
    29881 tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc
    29941 aaatgaactc cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga
    30001 gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg
    30061 acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga
    30121 cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat
    30181 gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga
    30241 taaagatatg gaagattaca cgaattttct attagagaca gattatgctg attttctgcg
    30301 ccctgtcatc cgtgagggaa atcttgatct cgtaaagctt gaacagttga aagatgatta
    30361 tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc
    30421 ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag
    30481 aagccatttc gaaccagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga
    30541 tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc
    30601 gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt
    30661 atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt
    30721 acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga
    30781 tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt
    30841 atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg
    30901 taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag
    30961 gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag
    31021 agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg
    31081 aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct
    31141 ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc
    31201 atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag
    31261 atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg
    31321 ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat
    31381 gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa
    31441 gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc
    31501 gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt
    31561 ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg
    31621 ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc
    31681 ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta
    31741 atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg
    31801 cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat
    31861 gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt
    31921 atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg
    31981 cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg
    32041 gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg
    32101 gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct
    32161 attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg
    32221 catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg
    32281 tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa
    32341 aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt
    32401 caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc
    32461 gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg
    32521 aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta
    32581 ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt
    32641 aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct
    32701 ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga
    32761 caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt
    32821 atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg
    32881 gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag
    32941 atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat
    33001 tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt
    33061 cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct
    33121 cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct
    33181 gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc
    33241 gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc
    33301 gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat
    33361 gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca
    33421 gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct
    33481 actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg
    33541 attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt
    33601 acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt
    33661 gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga
    33721 gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca
    33781 aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg
    33841 aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg
    33901 attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta
    33961 aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg
    34021 gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca
    34081 actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc
    34141 atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa
    34201 gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta
    34261 tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta
    34321 aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac
    34381 gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt
    34441 ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc
    34501 gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa
    34561 acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg
    34621 atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa
    34681 cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat
    34741 taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta
    34801 atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg
    34861 taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga
    34921 tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg
    34981 cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc
    35041 tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtta
    35101 tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga
    35161 aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt
    35221 tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg
    35281 ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc
    35341 agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat
    35401 taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga
    35461 atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat
    35521 attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga
    35581 tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg
    35641 tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc
    35701 gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt
    35761 tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca
    35821 tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat
    35881 tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac
    35941 gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa
    36001 aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct
    36061 agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg
    36121 caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt
    36181 ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat
    36241 atttttttat tttttctttt acgatactta gtccagaagc taaaatttca aggttattag
    36301 aggataggga aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat
    36361 gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag
    36421 gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa
    36481 gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat
    36541 ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca
    36601 catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat
    36661 aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt
    36721 taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg
    36781 aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct
    36841 ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga
    36901 caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct
    36961 cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca atgaaattag
    37021 cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga
    37081 tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc
    37141 cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt
    37201 ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa
    37261 cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta
    37321 cttcattggt agagatggtg atggactcaa agacattggg agaaataaat gtgccacttc
    37381 caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag
    37441 taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct
    37501 gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac
    37561 gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag
    37621 gcattttctc cactccttaa aattgtatac catacaattt taccattgat tgtattgatt
    37681 ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag
    37741 tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt
    37801 atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag
    37861 gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa
    37921 aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga
    37981 ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca
    38041 cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg
    38101 cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt
    38161 ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata
    38221 cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag
    38281 tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat
    38341 ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc
    38401 ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt
    38461 actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag
    38521 aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata
    38581 aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt
    38641 gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta
    38701 cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct
    38761 agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga
    38821 aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg
    38881 agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta
    38941 caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag
    39001 aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt
    39061 gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt
    39121 atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat
    39181 caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt
    39241 tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc
    39301 acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta
    39361 tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat
    39421 aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga
    39481 attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga
    39541 cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt
    39601 tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat
    39661 aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt
    39721 cagtaatctc aagaccggca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg
    39781 ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag
    39841 tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt
    39901 ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg
    39961 atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc
    40021 ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc
    40081 atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca
    40141 tggataaacc aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag
    40201 catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt
    40261 tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg
    40321 agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg
    40381 agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg
    40441 ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga
    40501 gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat
    40561 ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt
//
LOCUS       DACEOM010000018        39735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEOM010000018 DACEOM010000000
VERSION     DACEOM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.019"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(115..918)
                     /locus_tag="IW781_000913"
     CDS             complement(115..918)
                     /locus_tag="IW781_000913"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1181928.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(938..1780)
                     /gene="kduI"
                     /locus_tag="IW781_000914"
     CDS             complement(938..1780)
                     /gene="kduI"
                     /locus_tag="IW781_000914"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ1181929.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(1796..2803)
                     /locus_tag="IW781_000915"
     CDS             complement(1796..2803)
                     /locus_tag="IW781_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ1181930.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(2810..3454)
                     /locus_tag="IW781_000916"
     CDS             complement(2810..3454)
                     /locus_tag="IW781_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1181931.1"
                     /translation="MKKIDILSRLKNAGVIAVVREKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(3554..4312)
                     /locus_tag="IW781_000917"
     CDS             complement(3554..4312)
                     /locus_tag="IW781_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ1181932.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(4439..5590)
                     /gene="fucO"
                     /locus_tag="IW781_000918"
     CDS             complement(4439..5590)
                     /gene="fucO"
                     /locus_tag="IW781_000918"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ1181933.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(5672..5983)
                     /gene="rhaM"
                     /locus_tag="IW781_000919"
     CDS             complement(5672..5983)
                     /gene="rhaM"
                     /locus_tag="IW781_000919"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ1181934.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(5985..6830)
                     /gene="rhaD"
                     /locus_tag="IW781_000920"
     CDS             complement(5985..6830)
                     /gene="rhaD"
                     /locus_tag="IW781_000920"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ1181935.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(6844..8100)
                     /gene="rhaA"
                     /locus_tag="IW781_000921"
     CDS             complement(6844..8100)
                     /gene="rhaA"
                     /locus_tag="IW781_000921"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ1181936.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(8097..9557)
                     /gene="rhaB"
                     /locus_tag="IW781_000922"
     CDS             complement(8097..9557)
                     /gene="rhaB"
                     /locus_tag="IW781_000922"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ1181937.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            9710..10579
                     /locus_tag="IW781_000923"
     CDS             9710..10579
                     /locus_tag="IW781_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1181938.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(10705..11604)
                     /locus_tag="IW781_000924"
     CDS             complement(10705..11604)
                     /locus_tag="IW781_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1181939.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(11769..12593)
                     /locus_tag="IW781_000925"
     CDS             complement(11769..12593)
                     /locus_tag="IW781_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1181940.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(12604..13899)
                     /locus_tag="IW781_000926"
     CDS             complement(12604..13899)
                     /locus_tag="IW781_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ1181941.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(13915..14394)
                     /locus_tag="IW781_000927"
     CDS             complement(13915..14394)
                     /locus_tag="IW781_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1181942.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(14400..15428)
                     /locus_tag="IW781_000928"
     CDS             complement(14400..15428)
                     /locus_tag="IW781_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ1181943.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(15445..16551)
                     /locus_tag="IW781_000929"
     CDS             complement(15445..16551)
                     /locus_tag="IW781_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1181944.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(16780..19377)
                     /gene="adhE"
                     /locus_tag="IW781_000930"
                     /gene_synonym="adhC"
     CDS             complement(16780..19377)
                     /gene="adhE"
                     /locus_tag="IW781_000930"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1181945.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(19682..20062)
                     /gene="yajC"
                     /locus_tag="IW781_000931"
     CDS             complement(19682..20062)
                     /gene="yajC"
                     /locus_tag="IW781_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1181946.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(20159..21304)
                     /gene="tgt"
                     /locus_tag="IW781_000932"
     CDS             complement(20159..21304)
                     /gene="tgt"
                     /locus_tag="IW781_000932"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1181947.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            21522..22220
                     /locus_tag="IW781_000933"
     CDS             21522..22220
                     /locus_tag="IW781_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1181948.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(22388..25027)
                     /locus_tag="IW781_000934"
     CDS             complement(22388..25027)
                     /locus_tag="IW781_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1181949.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(25646..26677)
                     /gene="queA"
                     /locus_tag="IW781_000935"
     CDS             complement(25646..26677)
                     /gene="queA"
                     /locus_tag="IW781_000935"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1181950.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            26870..28045
                     /locus_tag="IW781_000936"
     CDS             26870..28045
                     /locus_tag="IW781_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1181951.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            28049..28690
                     /locus_tag="IW781_000937"
     CDS             28049..28690
                     /locus_tag="IW781_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181952.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            28687..29604
                     /locus_tag="IW781_000938"
     CDS             28687..29604
                     /locus_tag="IW781_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1181953.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            29601..30272
                     /locus_tag="IW781_000939"
     CDS             29601..30272
                     /locus_tag="IW781_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1181954.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(30813..31301)
                     /locus_tag="IW781_000940"
     CDS             complement(30813..31301)
                     /locus_tag="IW781_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1181955.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(31502..31768)
                     /locus_tag="IW781_000941"
     CDS             complement(31502..31768)
                     /locus_tag="IW781_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1181956.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(31768..32031)
                     /locus_tag="IW781_000942"
     CDS             complement(31768..32031)
                     /locus_tag="IW781_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1181957.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(32024..32296)
                     /locus_tag="IW781_000943"
     CDS             complement(32024..32296)
                     /locus_tag="IW781_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181958.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            32507..32692
                     /locus_tag="IW781_000944"
     CDS             32507..32692
                     /locus_tag="IW781_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1181959.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(32834..34657)
                     /locus_tag="IW781_000945"
     CDS             complement(32834..34657)
                     /locus_tag="IW781_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1181960.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(35255..37960)
                     /locus_tag="IW781_000946"
     CDS             complement(35255..37960)
                     /locus_tag="IW781_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1181961.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEVGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDNWSRFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(38266..39213)
                     /locus_tag="IW781_000947"
     CDS             complement(38266..39213)
                     /locus_tag="IW781_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1181962.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            39403..39672
                     /gene="rpsN"
                     /locus_tag="IW781_000948"
     CDS             39403..39672
                     /gene="rpsN"
                     /locus_tag="IW781_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1181963.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
BASE COUNT        11139 a         8672 c         7164 g        12760 t
ORIGIN      
        1 gcttctgtcc cagcctcttt tttcgaataa atgatgttta atcagctgtc cttcggtatt
       61 cgttcaatcc gctgatttca caacctctcc actaaacagt gtactaaagc tcttttaagg
      121 ttgttttccg atataggcaa gtatgcctcc gtccacatac aagacatggc cgttaacgaa
      181 atcagatgcg ctagaagcca agaagacaga tggtccagcc aaatcgattg gctcgcccca
      241 gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt caccgttttc
      301 ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata taacctggtc caattccatt
      361 acattggata ttgtattgac cgtattcaga agcgatgttt ttcgtcagca tttttaatcc
      421 gccttttgcc gcagcataag cactgaccgt ttcgcgtcct aattcactca tcatggagca
      481 gatattgatg attttaccgc cgcctttttc gatcatatcc ggaataactg cttttgaaac
      541 gatgaatggg gcatttaaat ccacgtcgat tacttggcgg aagtctgccg catccatttc
      601 gatcatcggt gtccgtttga tgatccctgc attgttgact agaatatcaa ctgaacctac
      661 atcttctttg atctgttgaa ccatcgcttg aaccgcttct tcatcggtca catcgcagac
      721 ataacctttt gcatcgatgc ctgcttcttt ataatgcgct ataccttcat caactgattc
      781 ttgattcaag ttattaaata cgatttttgc tcctgctgta gctaaagagc gtgcaatctc
      841 aaatccaata ccatagacag caccggtgac taatgctact tttccatcta aacgaaacat
      901 atccatacta aattccatca ttttctctcc cttatgctta ttttaattga tccatttgca
      961 ccatatccat gtctgtatag gtgatatttt ctccgcacat tgcccagatg aatgagtaat
     1021 tgcttgtacc tacacctgaa tggatcgacc agcttggcga aataatggct tgttcattgt
     1081 ctacaactaa atgttttgtt tcatctggtt tgcccatcat gtggaacaca cgtgtatctt
     1141 cattggcata atcaaagtag acataagctt ccatacgacg ttcatgtgta tgacaaggca
     1201 tcgtattcca agcgcttcct ggttctaaaa tcgtataacc catttgtaat tgacagcttt
     1261 cgcatacatt tggatggatg tattggtaga ttttacgttc attcagtgtc aggccttcac
     1321 cagtctccat tggttttact tggtcgatac tgatttttac atttggatat ttatgatgag
     1381 ctggaacaga actaatatag aatttagctg ggttttcagc atcttgagaa gaaaatacaa
     1441 catgtcgtgt ttcttttccg atatagtatc cgtcttgttt tttcatttct tctttttgcc
     1501 catcgatctc gatgaatcct ggtccaccga tattgatcac acctagttca cgacgctcta
     1561 agaagtaatc aacacctagt tctttgtcta atgtgatttc taattctttt tcagtaggtg
     1621 tcactccacc aaagatcatt cgatcgttgt gcgtataagt caaactgatt ttccctggtt
     1681 caaatacctt ttctactaaa aattcttttc tcaattgttc cgttgaataa tgacggatat
     1741 cttctggact gtgtgtataa cgtgtttcca tttcttgcat tttcttctac tccttctatc
     1801 gaatgatttt tccagagccg catgctaaaa attgtgcgac ttcttcttga ctaaattggt
     1861 tgcagtcccc atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa tcaatgattt
     1921 cttgcggaga catattggat aaaatcccat ggagcacgcc acctgcaaaa gcatctccgc
     1981 cacctactcg atccacgatt ggggtgattc tatggacttg ggattccaca tattcatggt
     2041 tcatccacaa tgtccccttc aattcatttt cacttgctga atgaacggta cgcttggtcg
     2101 aataaaacac ctgaatattc gggaactttt cctgcatttt ttgataataa taaattagag
     2161 gttgttcttc tgcttctggc gctttctcga tacctaacaa ataaagagca tccatttttc
     2221 cagctgagca gatatctact aaaggtaaaa gctgatggat tacttcgctg gcttcttgct
     2281 gtgtccataa ttttccacga tagttgatat cgaagctgat caggcagcca gctttttttg
     2341 cagcttctat cacctgtttc gtcatttttt gccatttttt agacaaagct ggggtgattc
     2401 ctgaaacatg aaatatatct attccttgga ataactcctc tagcgaccat tccaaatgat
     2461 ccatttcagc aaaacttgat ccggcacgat catagatcac tgaggcggcg cgttcgccac
     2521 taccagtttc catataataa gtacctagtc ttgatccacc agataatacc tgtttcgtat
     2581 ctactccata acattgcaag tgctttttga ctgcttttcc caatgcgtta tcaggtactt
     2641 ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga aattgctacg tttgcttccc
     2701 ctccgccata atgggcagcg aatcgttcac ttgaatcgat acgggttccc tgctctgtgg
     2761 acaatcgcaa catgatttct ccgagtgtca ctacttttcc cataagcagc tacctcttga
     2821 tttctttcat ttttgctgca tactgttgcg cgacttccgt tactttgtca aaatctcctg
     2881 tagctgcggg tgctaaaagg ttcccaccta ctccgactgt tacgacacca gcatcgaacc
     2941 actctttcat attgtccaga ctcacaccgc cagttggcat gatattgact tgaggcatcg
     3001 gcgccttgaa ggcagaaatg atacttggtc cataagcgct tccaggaaat agtttgacga
     3061 tatctacccc gcttttcagc gctgttttga tctcggtaat agtcatacat cctggtaagt
     3121 aaggaatttg atacagattg cacatctctg ccgtttcttg atcaaaggaa gggctaacaa
     3181 tatactctgc gccagctaag atagccaagc gagctgtgac agcgtctaat actgtgcctg
     3241 cgccaatcac tatttctggt tggtctttgt agaatgacaa aagttctttg attacttgat
     3301 ctgcttgagg gactgtaaaa gtcaactcaa taccagttag accacctttg atgattgcgt
     3361 ggcaagcatt caaagcttct tctttgcttt tttcgcgtac caccgcaatc actccggcgt
     3421 ttttcaatcg agataaaata tcaatttttt tcatcttctc cacctatttc ctatttagaa
     3481 attttatttt ataacttgga aatctatacc catattatac ggcagaaaag aaaaattgca
     3541 aacgcttttt tcattatttt tgaattcttt gctcgatttc gctttttgtt tgcttgaggg
     3601 cactgattat ttgttctttt gtctcatctg ttaaacgata tttcggcata cttacactaa
     3661 atgcaccaat gatttgttcg tttttcacga ttgaggctcc tatacaaaaa atatcacgtt
     3721 cgatttcttc atcgtcaaat gcgatgtggg tttcttttac cgtttggatt tcctttttca
     3781 accgtaatgc attggtgatc gtatattctg tgtaagggat caacggatgc ttttcaagat
     3841 aatcagtgta ctgctcttca ctgaattctg ctaatacggc tttgcccatc gcagaattgt
     3901 ataatggtcg ggtgattccg atttttgaag acatgcggat cgtttgatgt tttggatcta
     3961 gtttgttcac gtacaagatc tcattgttgt ccaatacccc taagtggata gtctcatcga
     4021 tcttttgctg cagctgttct aaaaacggta aagtcaactc taccaaatcg atttgttcaa
     4081 tgtttttatt cgcgtagcga ataagctttg cacctaaacg atagtttttc tctgaatttt
     4141 tatttacgta gccgatcatc aacaaagtat ctagtatttt caagactgta gaagaggtca
     4201 ttcccacacc ctttgcgatc gtctgcaaag agacatcttg gttttccgct aaatagtcta
     4261 gtattttaga ggctttgatc aatacagttc cataaggctt tttattttcc atataatatt
     4321 ctcctatttc tttataagaa aaattatagc ggaaatacca tcaatttaaa agagatatcc
     4381 agatataaac taccatttct ctttacaggc aatttaatgt tgaagatccg cttcgttttt
     4441 acatcaaact ttgatataaa gcaatgattt cttctactgt cgtttctctt ggatttcctg
     4501 gtgtgcatac atctcgcaaa gcatcttctg caattgctgg aatatctgct tcttttacac
     4561 ctaattcaga aatggtttct gggattccaa ccgctttgct caaccgatcg atctcaccac
     4621 aagccgcttc tcttacatct tctaaagaca tttcagctgc tcctttaatc cctagaacaa
     4681 gagcgatctc acggtacttt tctccagtat attctttgtt atatttcatg acagttggca
     4741 aaagtgctgc acacgctacg ccatgaggaa tgttgtacca tgcacttaac gggtgagcca
     4801 tcccgtgtac cagtcctaaa ccgacatttg agaagcccat tcccgcaata tactgtccta
     4861 atgccatttt ttctcgccca ttttgatcgc catctacaga aacttccaaa gaatggccga
     4921 tgatttcgat tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct tttgtgataa
     4981 acccttcaat agcatgcgtc atagcatcca tacctgttga tgcagccaat tttttcggca
     5041 ttcccatcat catatcgctg tcgatgaacg cgacgattgg gatatcatga ggatcaacac
     5101 aaacaaattt tcggtgattt acttcatctg tgatgacgta gttgatcgtt acttctgctg
     5161 ctgttccaga agtcgttggt atagctaata taggtaaaca tggatttttc gtatcagcaa
     5221 caccttctaa actaatgacg tctgaaaact ctggattcgt caaaataatc ccaatggctt
     5281 ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa acagtctgcc tctgcctctt
     5341 taacaaaagc cactccatct tttacatttt ggatcgttgg attaggtacg atcccgtcgt
     5401 ataaagcata agcgattgct gctttatcta gaagttctgt tacttttgtt gctataccgt
     5461 gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc tgctgaaatt
     5521 cagggacgat cgactcaatc gctccttttc caaaatacga tgtttcattt aaaatcattc
     5581 gattactcat tttctattcc tcctaatttg tattattttt cacaataaaa acgaaagaac
     5641 cccaagcaac cggttgccca gttgcttgtt gctacagttc aaagactggc atcaattccc
     5701 aactgactgg agaggcatct ggatttgtct ccatgatatc ttccatgtac ttccaccatt
     5761 tttgattgat agatgtattt gccatttttt cccataactt ttcatcttcg atcactaagt
     5821 aaccaaataa caaacttgta ctcggatcaa gatagatcga atagtcgatg accccgtgtt
     5881 cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg tctttttttg tattcttctt
     5941 gtttttctgg gtaaactttc atgcaaaaag cttttttcac catgtcagac acctgctttc
     6001 gtatgaactt cctccaaata acctgctttt ggagtgacac caaacgcatc tgctaaatgc
     6061 cacaggtttt cgtccgtgat tgtttgcaag attggtcctt gcgctttgac gtacgtatag
     6121 acttctgctg ccttttctgc tgtctcgatc aaaccaaaga cttcatccat gtcttttcct
     6181 gacccgtaga ttccatgttg tggccataaa accaatcgag tttctttcat tttttcagct
     6241 gttgcttccc caatctcatt tgttcccgga accatccacg gaatgatgga aattccttct
     6301 ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg tacgactaaa ggaacgttcg
     6361 tctaattcat gcgtaaaact catcgctaat agatgactag catggcaatg cataacgatc
     6421 cgattatctg ggtctacttc taaacgggcg atatgactca tgaaatgact tggcaactcg
     6481 cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt gccatcttca
     6541 gcaactcgaa cgattccaag attctcggca ggttcgctcg ctacattttt aaaatatctt
     6601 cctgaaccag ttacaataaa atagcatcct gctaaacggc tggcatcaaa aatcatcgag
     6661 atgcggcgga tcactttttc aggatccaag aacggtgtga tctcttcttc ttttagtaaa
     6721 taagaaatat tcccgccatt tctttcatcc catcccaagc gataaagatt cgacgtcgtt
     6781 tcgatcattt ctttcacata aggcgcttgt aaaatatcta tttttttcaa tatagccaca
     6841 ctcctatttt cttaattcca acacgtcttt ttgatattcc tggacttctt ccagccaatc
     6901 caaaccaact ggaacttgat ttttgtaaca gaaataattc catacatcgg catacgggaa
     6961 atctttcaat tcttccgtat aagccattct tgaagtaaag tcaaaagcca gctccatttc
     7021 tttcaatcgc tcaactggtt ccagcatagc tttcagcagt gctttttgtg tatttctcgt
     7081 accgatgacc caagcagcga tacggttgat cgttgcatca aagaaatcca gaccgatatt
     7141 tgttttttcc aacaattcgt tacggactaa ttctttagcg atttcctgca attcatcatc
     7201 catcaaaacc acatggtctg aatcccaacg cactggacgg gaaacatgca acatgattcc
     7261 tttgctgaat aaagctaatg aagataattt attggaaatc acttctgttg gatgaaaatg
     7321 tccagcatct agacaaacaa gtttgtcacg agtcaacccg taacccatat aaaattcatg
     7381 ggaaccgact gtataagctt cagcgcctaa accaaatagt ttgctttcta ctgcatcttg
     7441 tacataccgt tcatctaact cttctgtaaa aatttcatcg agtgattcca ttaaacgttc
     7501 ccgtggggta aaacgatcaa tcggattatc tttcatccca tcaggtaccc aaaagttatt
     7561 gatacttgtt tgcccagttt ctttcccaaa gtactccgcg attttgcggc ttcttttccc
     7621 gtgttcgatc cagtattcac gaacttcttt atctggatga gccaatgtaa agccgtcttt
     7681 catcattgga tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt ctttttttgc
     7741 ccactctacc catttttcaa aatgtttcgg ctcaatttca tttagatcga cagattcttc
     7801 tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg cccggtatca acgaataagc
     7861 cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa aattccctgt
     7921 cgccatgatt ccaccggtca attcgccatc cggtgtcaag aaacctttga catcatctcc
     7981 ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag catctgtatc
     8041 tacaccgata gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa tcgtgttcat
     8101 acgattactc ctttgatttt attcacgtac cattccatcg tgttttgctt attttcatct
     8161 gggtaaaaag tttcacatgg aaacgactgt ttgattacat ttcttgcttg ttctatattc
     8221 gaaaactgac ctgtactgag catttgcatg gcaacattcc cgattgctgt ggcttcacta
     8281 ggtcctgctt ctacaatgat tccggcataa tctgctgtca attgattcag tagacgatta
     8341 ttcgatccgc cccctacgat gtgcagcttt tccaatttcg ttccagttaa gtgttctact
     8401 ttgatcagct cttgtgcgta acaaagagca agattatcat acacacaacg agcgatagct
     8461 cccgatgaga cagggactgg ctgctctgtt tcaaaacagt attgctgaat ctcttctatc
     8521 atatttgggg gattcaaaaa tcgagggtca ttgatttcca tcacttgctg cagcggtttt
     8581 tcttttgcag caagctctgc caattccgca taactgtact cgtactcttg cattcgggca
     8641 acttcctgga tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt gttatgggca
     8701 ccccattcat tcgtataatt ttcagaaaaa gcattgcgag aaacattggc gatctttgtt
     8761 tcaatcccta aaagcgacca tgtcccactg ctgatataac cccaacgttc tccttgacct
     8821 ggcgttccta atacagctga cgccgtatca tgacttgcta ctgtgataaa ggtcacatct
     8881 ggcaaatcat attttgaaaa ttgttctttt tgcagatctc ctagtatcgt tcccggctct
     8941 gtcaatggtg gaaataattc cttcgagaca ccgatttttt caagaagttc tatttcccat
     9001 tcttttgtcc ctgcattcag cagctgcata gtagatgcat tagttttctc cgtcaccatg
     9061 ttaccagtaa agacataccc taaataatca ggtatcagta acaatttatg tgcttttgcc
     9121 aaattttctt tttcttcagc aaacaattgg aacaaggtat tgaaaggctg gatctgaatc
     9181 cctgtctgct catacaactt atctagagaa tagatttcag aaaactgttc tactgctcca
     9241 ttggtacgct tgtcccgata agcaatcgga tcatttatcc gatccccttg ttcatcaatc
     9301 agacagtaat cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat ccctgtctgc
     9361 tttaattttt caaatccaat caaaatctct ttgataagat aatcgatctt ccagcgatcg
     9421 tgaccgtctt tgttccgaaa gccgtttttg aaccggtgta cttcttccaa gatcaaagaa
     9481 ccttttgcat ccctcgtact tttcattagt cgtccgcttg atgcaccgat atcgatggca
     9541 acaaatgttg gattcatttc tccacctctt ttactttatt gttgattagc actttattaa
     9601 taaagcgtta tcatttatta acaatgctat cataatacag aatcttatat tttgcttcac
     9661 actattttgg tatataattg cattatcttg acaagttggg agtgtttaca tggattactg
     9721 ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga cgcctacaca
     9781 aagaagcagg aatttatttt tttatatcca agaaatcggt tacttcaaag caccaatccc
     9841 ttactatact gaacgagcac atttaccttc ttacttgata aagtttactt tgtctggcaa
     9901 aggttcgctt acatataatg ataatcagca acagctaaaa gctggagact tattttttat
     9961 tgactgccaa gaatatcagc gttatcaaac gatcagcgaa gaaccatggg aaatggattg
    10021 gatccatttg aatggtcaga acgtccaatc attttatcag gagttcacaa aagacggcca
    10081 taacaccttc cattcaacag gggatccatc agaaaacaag atccaccatt tgatcacaga
    10141 attgatccac atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct ctgtagctat
    10201 ccacgagttg ctgaatgaac tgattttgca aaagtatcag ctagatttcg aagaagagga
    10261 tattcccgcc tacgtttaca aactgaagca atacttagac gaacatttca aagaaacgat
    10321 tacacttgat caattagaac aacaattctt attgaataaa tatcagctga gcaaagattt
    10381 ttccaaattt atcggttctc caccaatcga ttatttgatc agcaaaaaac tttcttacgc
    10441 taaagacctt cttcggtata cagaatttac ggttcgaatg atttccgaag aaatagggat
    10501 tgagaatttt gcttacttta gccgcctgtt caaagcaaaa acaggactat cgccgagaga
    10561 ctttcgtaaa ttcagctgat tcatataata tgtacgtcat tttttcaaaa tattttgaaa
    10621 tatatagata aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac agcagctact
    10681 gcggttattt tttagtgatc attctcattg ttcatcttca aaatgttcat aatgaaccaa
    10741 ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact ctctcttgaa
    10801 tgctttcaga aaatgcggat aagaagaaaa accactttta ctatatacag cactagagct
    10861 gcggtattct cgcaacaagt gcttggcata gatcagcttt ttcttcatta catactgatg
    10921 aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata tggtatttgc tgacaaaaaa
    10981 ctgcttttcc atttcctcta aagaaagcgg atgagataaa ttttttgaga tatattgtaa
    11041 catttgttcc acacgctcat taggaactac agcattctgt tcttgcgcac tctcttcttt
    11101 ttgtacaact tgattgagaa aaatcaagta ttctaccaga gcttgttcat attttatgtc
    11161 ttcaccgtaa atttttttat caggaggatg gtcgataaat gataattttt gcttcaatac
    11221 ttcctgttct atttgcaata cacgactcct cttctctccc attggttgaa aacaagccga
    11281 tagattagac catggcgtgg aacgactttc tagaaaagaa ggggtgacaa agatatagac
    11341 acgctcgaat acatctgaag attggcggac gatacgatgc aaatcattgg aatggatgag
    11401 cagcacactt cctgctttga tcgtaaattg tcgtccgttc aaataaaaag tcgcttctcc
    11461 ctttaatgta gcatggatct catagaaatc atgatgatga tagatcgttt tattttggct
    11521 gacgacatct ttgacatgat agatttcata ctgatcagaa accatcgtat tgacttgttc
    11581 accgctgatt ttcttttgga gcatgtgccg cacctccttt tgttcactta tgcacaattt
    11641 tattatacca cgttttttgt aatcaaaaag agctgctttt atatactctt ataaaaaaga
    11701 agagacgtgg catcttctag ccccgccccc tccattctaa atattagtta accaacgtct
    11761 tctttacatt attcaagaag acttcttcca taacgaatcc catcatcttt tgtttcttca
    11821 agaaccacat acaggtacgg acgattcttc atgatgattt ctttgatttt tgtgtactcg
    11881 atcacaccgt ctcccaaatt taccggcacg attttttcct gttcgacacg aaaatctttc
    11941 aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag cttcttctac caattggact
    12001 tgttcttgat aatcactact tgtgatcaaa ttggtcggat ctaaaatgat tcctagatag
    12061 tccgaatcta ctgcttggat cagctgctct acccttgcta aagaatacac cggatgattc
    12121 aaacctggtt ctatccccac catcatttgg tatttttctc cagcttttac caagcgacgg
    12181 ataacggaca cagcttcagc aaatgcttca tctgtgaaat tttcttccgt atattggatt
    12241 tccggcaaaa cacatccagt ttccgaagca accatcgacg caccaaagga agaagcatag
    12301 cgaagatact gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc tggatgaatc
    12361 atattgatat agcaacttaa aattcctacg gtcacttctt gcttttctaa aactcgtttg
    12421 acataatttc ccatcccaga attgatctca tttgcgccag aaggtgtttc aggaaacgaa
    12481 agacctaagg cgagctgcag tgtatgaatc ccttgttctt ttgttttttc tgcaagatct
    12541 tctatcgttt ggacttcgga aagatcatgt cctctaattg taatgttcaa cataactatt
    12601 ctcctaactc agcatattgg gtagccacat gacgatttgc gggaagtaag taatcaaaaa
    12661 caagacgact aagataaccc cataaaaagg aagaagcggt ttcagcattg cctctgcttt
    12721 tacgcctcct acacttgcac cgacatatag tcctgtacct actggcggcg tgatggagcc
    12781 tacacacaga ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc ctacacttgt
    12841 gacgattggt aaaaatattg gtgtaaaaat caagatagca ggcgccacat ccataaacat
    12901 acccaccagt aataacacaa gattgactaa taacagaata ataaatttat tatccgttac
    12961 gcccataatc aacgaactaa ttgcctgagg aataccggta aacgccatgg cgaaagacat
    13021 catggaagaa gtagccaata agaacatgat cgtccctgac attgctactg attgaaacaa
    13081 catattaggt atatctttta gttttaccgt tttatagtaa aacatagcaa tcaacaggga
    13141 gtaaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca agataccacc
    13201 gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt ttccagcatg
    13261 agccatctgt cctttttcga caacagggta tttattcttt ttagcaacga tgaatgctac
    13321 tagcatgatt cctagcgccc acaaagcgcc tacgagataa cctcccataa acaaagcaga
    13381 gatcgaagca ccaccagcaa caagtgaata catgataaaa gcagtacttg gaggaatgac
    13441 catacctgtc ggagctgacg cgatattgac cgctgtcgca aacttccggt catatccttc
    13501 ttccacttgc tgagggatca acactccgcc aatcgcggta gaagcggcaa tggccgagct
    13561 agagatggaa ccaaataaag cattccctaa cacattcgta tgagccaaag cccccggcac
    13621 gcgcccgacg aacaacatcg caaaatccac tagtttctta gctatccctc cattattcat
    13681 gataatgccc gagagaacga aaaacggaat agcgatcagc gaaaaactat tgatacttcc
    13741 caccattttt tgagctgaac taaaagcaga aacatcaaac ggtataacta acaataaagt
    13801 catcatcgat gagaatgcaa tacttatagc aatcggcatc cctaatacaa gcaacacaac
    13861 aaatacaaaa aataacacta atccggcttc tattgccatt ttattcctcc atttttatat
    13921 tttttcttga attgctggct tgttgcttaa tgtatcgatt agattgatca ggctatagat
    13981 aatgatgagt acccccgata caggtactga cagataaaca tatcccatag gaatacttaa
    14041 agaaggcgag atctgcccca ttgtcaatgt gacagcattc catccgccat acaccatgat
    14101 catcgcagca aataccagaa ataaactttg aacaacaact tctaaaattt tacttttttt
    14161 gccagttaat ttttcactta aaaccgttac ggctaaatgt gctttttgtc ccacaacata
    14221 cgcggaagac aacatggcaa accagacaag accgaatcga acaagctctt ccgtgaccgt
    14281 atttggattt cctaaaaccg ttctggaaaa tacttgccat aaaacaacag cgaccatccc
    14341 aacaagcaaa acagctccca ggatctctaa taccttgttt aaaatcattt tcatcacagc
    14401 tacgctcctt gctcttggat cttttgataa aggtctttgt aggtaggatc gtttttgaat
    14461 atatcatgaa gtggggcaac tgcctctcga aacggtgtat tatctacatc attgaattgg
    14521 actccatgtt cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc aaaagcttca
    14581 atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg ctttttcctg ctcatcagtt
    14641 agctgttctt tgatggcatc attcatgatg atgatatctg gcacacgtgt atgctcatca
    14701 taggaatagt atttagccac accaccatgt ccagctgtat acaagacaaa ttcattgttt
    14761 tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca cttcatcact tcccataggt
    14821 acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg catcacacgg
    14881 atcttctttc cctccaaatc ttccggcgta tggatcggac cgtctaccat ataaaagctg
    14941 cgctgaccag aatcataata ggtcaatccg gtaaatccta aatttttggt cgattggtag
    15001 attggctcca ttatttcttc attatccatc accttaaaga aatgttcttc atcattgaat
    15061 agataaggaa ctgaaaaaat cgaataatct ttagagaaac tttccaacgc tgaggcactt
    15121 acttttgtga aatctactgc tcctgactgt gtcaattcga tcagttcacg ttctccaccc
    15181 agttgtccat ctgggaaata ctggatctgt acggaaccat ctgttttttc ttttactaaa
    15241 cgcccaaatt cattcattgc atcaattact ggctggctgt tgcttgcata agcaaagcgc
    15301 aaggtaacta tctcatttac atctgcatct gcgacagttg ccacactaga attatttccg
    15361 cagccgctta gacctagcag acctaggcta agcgaacttg ctgcaaacag cagtgatgtt
    15421 tttttcatat gggtttcctt tcttttaaca ataagagacg agcaattctg tcaaacaaag
    15481 caccgttaac gactgtccat aaggcattgc tgtgattccg atattccgat aaaaatctaa
    15541 actgtccccc attcctgtcc cgattgaaac gttttgaact tctccttttt catcgatttg
    15601 ttcaagcaat ccttggatag cacgataagc gacttcttta tattcttgtg gtaaataacg
    15661 tttatggacc gctttcaaaa taccgtaagc aaatcctgca gttgcagaag attctacgta
    15721 agaagaagga tcatccaaca acgtatgcca taaaccgctt tcagactgat aagtttttaa
    15781 tgcacgcact tgtgcttcaa gtgtttcgat caataatttg cgcaatgcat cttctttcga
    15841 taattctaag atttcaatga tctctggaat agcaatcgtc aaccagcagt ttccacgagc
    15901 ccaaaatgct tcggcatagt tatgatttcc ttcaaacgtc cagccgtgat accacaatcc
    15961 tgttcgttta tccatcaagt atttgatatg gatcataaac tggtaacgag cttcttctaa
    16021 atattctggg cgattcaaca gtttcccgat ttttgccaaa ggaagcactg tcatcatcaa
    16081 tgtatcgtcc cacaactgat tcttattttc cggtccatat gtggcatgct gtaacccgtc
    16141 ttcatttgtc cgaggcatgt cattcatgac ccattctgcc cattgttcta aataaggcaa
    16201 atagcgtgaa tctttcgtgt cttcatacaa gtaagccata gtcaatagcg gtgccattgt
    16261 attgacgttt ttgggcggag cgccttcttc catacgatcc tcaaaccatt cattgaccac
    16321 ttgataagcg cgaggatttt ttgtcatctg gtaattttta taaataccat atagaccgac
    16381 accttgtggc caattccaca ctgtccagct tttgtcatct actttcagtc catcaaagtc
    16441 caacaaaaat tcaccattat cgtctttgat cgttgtcagg ttttcaacga gcaggtcaat
    16501 tttttctttc actaattctt taggtacaaa aatctcttct ttaataatca tctttacgcc
    16561 acctttttaa acgctttcat tttatatcct taatatagca gtaataccgt ccactataaa
    16621 tatcgctttt tgaacaaaaa ctgtctaaaa ttgacctaaa tgttaaataa caaaagtaac
    16681 tttcagttac ttggaaatta aaaaaagaac ttggagaaac aacgttttcc aagttctttt
    16741 tcatctattt tcttatcttt tttctacgct ttcttttgct tatccgttgt aagcatcaat
    16801 gatgatctgt ttcaattcag agatcaatgg ttctttcgga ttagctgtcg tacattggtc
    16861 ttcgtaagct aattcagcca tacggtctac ggttgtatct agtgtttctt gtgtgactcc
    16921 ttgagctttt agactcatgt tgatgccgac agattttcct aattcataaa cagccgttgc
    16981 taaagcttcg actaattcct ctgttgtttc gcctttcaga cctaagaatt ttgcgatatc
    17041 tgcgtaatct gtatctgcac ggaaatagtc atatttaggg aacatcgcat gtttttgtgg
    17101 atcttttgca ttatagcgga taatatgcgg taaaagaatc gcatttgtcc gtccatgagg
    17161 gatcccgtat tccccgccga ttttatgtgc gactgagtga caaataccta ggaatgcgtt
    17221 agcaaacgcc ataccagcca ttgtagaagc gttgtgcatt ttttcacgtg attccatatc
    17281 aggtgttttt actgattttt ctaaatagtc aaagactaac ttgattgctt gtaggctcaa
    17341 tccacgtgta taatctgaag ccatgactga aacataggat tcgattgcat gtgttaacac
    17401 atccatcccg gtatctgctg tgacagatgc tggaactgac atgacgaatt gaggatcgac
    17461 gattgcgaca tctggcgtca atgcataatc tgctaacgga tatttcacat gtgtttcact
    17521 gtcagtgatg accgcaaatg gcgttacttc agaaccagtc cctgatgttg tcgggataca
    17581 tacgaattgt gttttttctg gtttgtcgat cttatatgtc cgtttacgga tatccaagaa
    17641 tttttgtttt gcaccaaaga aagatgtgtc tgggtgttca tagaacatcc acattccctt
    17701 agctgcgtcc attgcagaac cgcctccaag agcgattact gtatctggct tgaagtctgc
    17761 catcagtttc gttcctgcgt atacggtatt tgtcgatggg ttcggttcaa catctgagaa
    17821 gacttcgatc ttcacatcat ttttacgtcg tgccaatact tcacgaactg tatcgcagta
    17881 accgaattgg accataccag ggtcacaaac gatcatgaca cgttcaacgt tttccatttt
    17941 ctctaagtac aatagagagt tcttttcaaa gaagattttt ggcggtaatt tgaaccattg
    18001 catgttattt ctccgtttcg ctacagtttt gacattgatt aagtttacag cagagacgtt
    18061 acgagaaaca gagttcttac cataagaacc gcatcccaaa gtcaatgacg gaatcatttc
    18121 gttatagata tcaccgatgc cgccttctgc agatggtgta ttcactaaaa tacgacaagc
    18181 tttcattcga agaccgaatt tgacatgcaa ctcttcgttt tctgaatgga taactgctgt
    18241 atgtcctaat cctccaagat tcaacatacc ttcacacaaa tcaaacgcat ggtccgtgtt
    18301 gttagctttg atcatggcaa gaactggaga aagtttttct cttgatagcg gataatctgg
    18361 gcctacgcct tccaattcag caatcagcat tttcgttcct tttggtacac tgattcctgc
    18421 caattttgcg atttccatcg cagaatgtcc aacgattgat ggattgaccg catattttcc
    18481 ttcgttcatc actgcatctt ctaatttttg caattcatct ggttttacga tataaacttg
    18541 gtgtgcttgg aattctgctt ttactgctgc atagatttct ttatccacga tcacagcttg
    18601 ttcagaagca caaatcattc cattatcaaa tgttttcgaa acgatcaaat cgttcactgc
    18661 acgtttaatt tttgctgttt tttcaatata agctggtacg ttacctggac ctacacctaa
    18721 agctggtttt cctgtagagt aagcagactt gaccattcca gctccacctg tcgctaagac
    18781 gatcgcgata cctggatggt tcattagcat agaagtcgct tcgatcgaag gatgttcgat
    18841 ccattggata caattttcag gagctccggc agcgatagca gcatcacgta ccactcgtgc
    18901 tgcttctgca gaagattttt gggcacttgg atggaaagca aaaacgattg gatttcttgt
    18961 tttcaacgcg atcattgatt tgaaaatcgt tgtggaagtc ggattcgttg ttggagttac
    19021 tccgcaaacc acaccgactg gttcagcaat ttcaatcaac cctttttgga catcttcatt
    19081 gatcacacca actgttttat catgtttgat attatgccaa atatattcgg acgcatacat
    19141 atttttgatt gctttatctt cgtaaacacc gcggcctgtt tcctctacag ccatcttcgc
    19201 taatggcata tgttgatcca aagctgccat tgccatttca tgcacgatat ggtctacttt
    19261 ttcttgatca aagctttcca tttctttcaa tgctgcattt gctttttctg ccaaatcatc
    19321 aatcattgct tgtacgtcaa ttgtcttcgt tttttctttt tctattactt tagccatcat
    19381 tatcctccta atgggtcatg gttatttgtt aaataattca catgtatatc atacaatatt
    19441 tatttttcta tgtaaacact tttttgtgat ttttttcaca gttattttta aatataaaat
    19501 tgctttattt acaggtgttt gtatagaaaa atatataaag ttatttatta ttatttatat
    19561 gacacacccg attattcaat attgagcaat atatcccatg ctcttatctc tttctttttt
    19621 gaataaaaaa tgaggtagga catctttctg ccctacctcg tctttaaata agctttgata
    19681 cttatttttc ttccttctct tctgtctctt ccagcgtttc tgttgtttca gtttttactt
    19741 caactgtctc agctggttct tctgttgttt caacagtagt cgttgcatca tttgttacga
    19801 cagtccctgg tttgaccgtt ttgattgctg cacgatcgta ctcaaggaag atcccttcgc
    19861 agtctaaaac aactgtccgt ttgtcattgt cgatttctga aacaactcca tgtaatccgc
    19921 cgatcgttac gactgaatca cctggtttca tgctatctaa caagttttga cgttcttgct
    19981 gttgtttctt ttgcgaacgt gacataaaaa accacattgc taatagcgcg ataaacatca
    20041 caagcattgg taatgttccc atttttttca cctcactaat tctgtatcta caaaaaacac
    20101 tttagcagaa aaatgctgat aacactagtt ttttacttaa tttttaaaag attttccttt
    20161 agaagctttt tgcgttttct ttattgaacc cgtattcttc aaaaaaggct tctctaaatt
    20221 caagcaaatt atcatccatg attgcttgac gtacttgttt cattagattc aacaagaagt
    20281 ataaattgtg gtaagaagtc aaacgaatcc caaatgtttc atcacatttg atcaaatggc
    20341 ggatataggc acgtgtatag tttttacatg tataacaatc acatttctca tcaattggac
    20401 ggaaatcatg ggcatatttc gcatttttca ctactagacg gccttgagaa gtcatacatg
    20461 tgccatttcg agcgattcga gtaggcaaga cacagtcaaa catgtctact ccacggataa
    20521 caccatcgat caatgaatca gcagcaccga cacccatcaa gtaacgtggt ttgttttcgg
    20581 ggatcaacgg tgtagtgaat tctaatacac gattcatttc tgatttaggt tccccgacag
    20641 ataacccgcc gattgagtaa ccagggaaat ccatcccgac tagatctttc gcgctttgac
    20701 gacgaagatc ttcaaaccct gctccttgga tgattccaaa taatccttga cggtctggat
    20761 tagcatgagc tttcaatcct cgttctgccc atcgagacgt acgttctact gattttttca
    20821 cgtagtcgta actttcatcg aatggtggac attcgtcaaa actcatcatg atatcagagc
    20881 ccaatttatt ttggatgttg attgcttttt ctggtgataa aaacatacgc gaaccgttca
    20941 agtgattttt gaaatgcacg ccttcttctt cgatattccg catatcactt aatgaaaaaa
    21001 cttgaaagcc gcctgaatct gttaaaatcg gttgatccca gttcataaat ttatgcagtc
    21061 cgccagcttc ttctactaag tcttcaccag ggcgtagcca taaatgatat gtattactta
    21121 aaataacgcc agcacccatt tctttcaact cttctgggga cattgttttt actgtagcta
    21181 atgttcctac tggcatgaac attggtgtgg ggaaagttcc gtgtggcgtg atcaattccc
    21241 ctaaacgcgc gccagtatgt ttttctttct tgattaaacg atagcgaatg gcaggttgcg
    21301 tcattcattt ccctactttc tatataaaat atcttgacaa ctcccaccat cataatcgtt
    21361 tcaagtgctt tttgcaagtt ccctcggctt ccttctactt tatttaatat tctttttttt
    21421 atcctttttt tatgaataac aaaaaaaaga aaaacattat gacagaaata ttttttgata
    21481 catggtatat tggtgttatt aaagaaaagg agggaaaaat catgcaatct tctgcagaat
    21541 taaaacgcca agcaaaagag agtctaaaag gacgttgggg acaagctgtt ttactaaatc
    21601 taatccctac attgattggt attgctacta ttcttcttat tatgattcct attatcttgt
    21661 ttgccaccac catatatgat ggatcagcga gcgtctcaga agcgacaact tcaggaggcg
    21721 gtagttcagg aggcggtggc ggaattattt ccagcatcat tagcgctttg ttcatgagcg
    21781 gtatttcttg gacttatctt gatattttac gtggacaaaa agatcagatc gaaccattca
    21841 aagatgcatt tcgtggattc aaaggtttgt ttatcggcgg agtgatcagc ttagctgtgc
    21901 tgatctcgat ctttacaaca ttatggggtt tgctattagt tattcctgga atcattaaaa
    21961 gctattctta ttctcaaagc tattttattt actacgatat cgtccaagaa acaggacaaa
    22021 cacctaaagt actggatacg atcactgcaa gtcgaaaatt gatggatggt tataaaggac
    22081 aacttttctg gttagatctt tcatttatcg gctggcatat cttagcatta gcaactgctg
    22141 gtattggtta tctatggctc agcccataca tttctgcaac aaaagcagca ttttatgaac
    22201 aattgccaaa agatatttaa aagagagtgt gacttgtgac cgagggggtg gcaggagaac
    22261 accgtttatc gagaggcagt aaaagaaaca ttcagcccat tagcaaaaag gaggtgtgac
    22321 aacattcatg tcacacctcc tttttgtata gttccagtaa aaagaaaatc aacttagaaa
    22381 cagttaatca ttcctcttct tctgcttccg gtaatcgatg gaacttgatc agttcattca
    22441 ccgcgattgg tacgatagat aaaatcccta ccaagaacca gtgattcaaa ctgatgtgta
    22501 ctaggccgaa tagttcttgc gtaaatggca gcaaagcaac agccgttgta atagctaatg
    22561 ccagtaacgc catttctacc aaagctttat tggtcgctaa attgactttg aagatcgatt
    22621 ggctgctgcg tacattgaac acatgcagaa tcgatgaata agctaagatc aagaatgcaa
    22681 cagtttgacc gacatgctga cttgcgtcta cccatgggct gatagtatcg acataactac
    22741 ctaaataata accaaacaag gttacgatgg tgaagacccc tgcatttatg gcaatttttt
    22801 gcaataaacc tcgagcaaag atcccttcat ttttcggaat gggtggctga tccatgatgc
    22861 cgtgttccgc tggctctctt cctaaagcaa atcctggaag tccatcagca actacattga
    22921 caaacagtag ttgcacagct gtaaatggtg ctccccatcc taacagcatc gcaatcaaaa
    22981 caataaagat ctccgaaata ttacagctta gcaaaaagtt gatagcttta cgaatattct
    23041 gataaaccga acgtccttga ctgacagcgt ctacgatcgt cgcaaagttg tcatccgtca
    23101 aaatcatatc tgcggcactt tgcgccacat ctgttcctgt gatccccatg gcacacccaa
    23161 cgttacttgc tttcaacgct ggtgcatcat tcacaccatc cccagtcatc gctacgactg
    23221 cacccgtacg ttgccaagat ttgacgatcc ggatcttgtc ttctggcgtt acccgagcat
    23281 agacagagag agctttgaca cgcgcatcta attcttcgtc tgataattga tgcagttctg
    23341 atcctgaaag tgcttctgac ggatcattca agatacctaa ttctttcgct atggcactgg
    23401 cagtcaccac atgatctcca gtgatcatga ctgttttgat gccagccttt ttcgctcgct
    23461 tgattgctcc tttactttct ggacgtggtg gatcgatcat tccgatcaga ccaatcaaac
    23521 gtaagtcttt ttccagtgct tcagaagaaa tatcttgcgg ttcttcgtca taagtagcat
    23581 aaccaacagc aatcactctc agtgcccgtt taccaaagcg atcattaacg atcgccgctt
    23641 gttctacatc gcctgaacta aagcgcggag caagaacatc gaatgctcct ttggtgattg
    23701 agatgtattt ttcgcccatt tggtgaactg ttgtcatcat ttttcgttca gaatcaaaag
    23761 gaagctcgtt tactcgtgga tatttttctt ctaattctgc ctttgtatga taattctctt
    23821 caacagccct tacgatggct acttctgttg gattaccttt gataacaagg tcgtcatctt
    23881 cttgctcgac cgtcacatct gtacatagtg cagccatttt caatatttcc atcgcgctgt
    23941 tcgtcattga atcttcgata ttcgttactt catcttcttt gctccatacc cggcggacgc
    24001 gcatttcatt ttgtgtcagc gttcctgttt tgtctgagca gataacatta gcggttccta
    24061 gcgtttcaac tgctggcaac tgtcggatga tcgcatgttt cctcgccatc ttctgcacac
    24121 cgtatgcaag tgtcaacgta acaataacag tcaaggtttc cggtacagct gcaacagcca
    24181 aggaaatcga agtcatgaac atctcaagga gtggttctcc ttgcagttca ccaatcacaa
    24241 atactaaagc cgcagccgct aaagcgatca tactgattcg tttgcctagc tgattcaatc
    24301 ggacctgtaa aggtgttttt tgcatagtat tttcgttcaa aagatccgcg atcttaccca
    24361 tttctgtagc catgccaatg gcagtgacga tcgctttccc acgacctgca gcaaccgtac
    24421 atcctttaaa taccatatta agttggtcac ctaatgactc atcttcttca gcaataaatg
    24481 ctgcatcttt ttcaactgct tcactttctc cagtcaacgc cgattcttct acacgtaatt
    24541 gtgatgcttc gatcaaacgg gcatcggcag aaatagcatc accggactct aaaacaagaa
    24601 tatcacccac taccagttgc tctgactcaa cagtggtcac tttgccgtct cgtatgacag
    24661 ttgccgtttg tttattcata tcttgcaagg attccagtgc tttttctgcg tttccttctt
    24721 ggacaatcgc caataccgag ttgatcacaa cgatagcgat gatcagcagt ccttcaaata
    24781 aatccccatg ttctgtagca aatgcggtat aaaaggaaat caccgctgct acaagaagga
    24841 tgatcgttgt aaaatcggac aagcttcgaa agaatttttt gatcatcgat tctttcggcg
    24901 cctcatcgaa tttattcaat ccctgctttt ttcgccgttc atccacttct tgtgaagaca
    24961 acccagtatc tggatctgct tctgttacct tctttacttc ttctattgtc tgcttgtatt
    25021 cttccatcct attcctcctt taaaatcttc gtacaaaaaa aggtacttat tttactataa
    25081 catccttttt ttatttactt caaataaagg gcttatcgat tatttgttaa gaaagcgtaa
    25141 aatttaaagt gcttatattt gcaaacgcta ccaaaggatg ctattatata gatgtaagga
    25201 aggaggatac catcaaatag acagaagaat ttctcgatag aacagtgtaa gcaaacaaag
    25261 ttgttagaag tacaggtttc acccagacat gcgtgtagag tacaaaccta taaatagtga
    25321 atgcgtatgg atgtttggat cacgaagtat ccgaaacaag aagtaatgcg atacgtaaca
    25381 attgaataac gtaaacgtct gaaaattgaa taggaacaag agatgtttca aatgttgaag
    25441 atgtaacgaa aacaaaattt ttgtttaaca tgaagaaatt caacaccgtg ctttgcatgt
    25501 atgatggaaa gttacaatca tttctgttta tttgaatcct tccgaggtac aagaaagccg
    25561 agcttatcga gagctcggct ttttctttgt cataaaatct ttgtaaagct tttttgttat
    25621 tcaaaactag cggcttttca taaccttatt tcacgaacat cgcatctcca aaactaaaga
    25681 aacgatagcg ctcttcaatc gcatgctggt aagcagataa agtcaattct cttcctgcaa
    25741 aggcactaac taacatgact aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt
    25801 ctaccacttt gaattcatag ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt
    25861 tgatttctcc attaaatttc gttccaatcg tttctaatgt tcgaattgaa gttgttccga
    25921 cagccacgat tttcccgcca tttttacgta cttggttcaa tcgctcagcc gcttcttccg
    25981 tcaaacggta gaattcacta tgcatatggt gttcttcaat tttatctacg ctgacaggtc
    26041 ggaatgtacc taaccctaca tgtagtgtca gatagactaa ttcaacaccc tttgcttcga
    26101 tctcatcaag taattctttc gtaaaatgca atccagctgt tggcgctgca gcagaaccat
    26161 tttctttcgc atagacagtt tgatagcgat ctgggtcttc cagacgttct ttgatatatg
    26221 gaggcaatgg catttctccc aatgattcaa gaatctctag aaaaattcct tcatactcaa
    26281 agtcaatgat ccgtccgccg tgttccaatt cttctttcac tactgcttgc aatctgccat
    26341 caccaaaaga gattcttgtc ccaactttgg cacgtttcgc aggtttgatc aatgtctccc
    26401 acgtatctcc ttgtgtattc gttagaagaa ggacttctaa atgcgcacca gtatctggtt
    26461 tttctccata taaacgagca ggcagaactc gtgtattatt cataactagc gcatcaccag
    26521 gctgtaactc ttcaatgatt tcatggaaat gtttatcttc catctctcca gttttccgat
    26581 ccaaaacaag taaacgagaa tgatcgcggt cttttaatgg tgtttgtgcg attaattctt
    26641 ccggcaagtc aaaatcaaaa tcttctgttg ttaacatatc gtcactcact ttctatcatg
    26701 tgcttcatca ttctatcatg tttttcgttc tgttggcaaa atcagcactt tttcacttct
    26761 attttttgtt ttcctaaata aaaaaactac cctatttgta ttttttcatt gttttaggca
    26821 tactagtaat agatagttct tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt
    26881 tcaaaatgtg tcaaaaattt ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc
    26941 atttgaaaag ggtgagttca tttgttttat cggtacgagc ggaagcggaa aaacgacgac
    27001 gatgcggatg atcaaccgaa tgactgatcc aacacaaggg aaaattttga ttgatggaaa
    27061 atccatccaa gaaattgatc cagtggaatt gcgacgtcag atcggctatg ttatccaaag
    27121 tattggattg atgcctcaca tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt
    27181 gaaagtcagt caagaagaac ggaataaaac agctgaaaaa atgattgatc ttgtggaatt
    27241 gccccgtgag atgttagatc gctacccaca tgaattatct ggtggtcagc agcagcgtat
    27301 tggagtcgtt cgtgcccttg cagcgaatca agatatcatc ttgatggacg agccattcgg
    27361 tgcgcttgat ccaatcactc gtgattcctt gcaagacctt gtcaaagatt tacaagagcg
    27421 tttgggaaaa acgatcgtat ttgtaaccca tgacatggat gaagcgatta aactagcaag
    27481 ccgtatcgcc atcatgagtg aaggccgtgt gatccaattt gatacaccag aaaatatttt
    27541 acgtcatcct gcaaacgatt tcgtagaaga gttgattgga gaagatcgcc tactacaagc
    27601 caagccaaat gcgacaagag tcggcgaagt tatgctaaat acagcaatca cgattacacc
    27661 ggaaaaatca ttacaagaag caatcaaact gatgagggaa aaacgtgtag atacgttgtt
    27721 agttaccgat aattcgaatg tattaaaagg atttatcgat gtagaaacgt tgaataaaaa
    27781 acgaggaaaa gtatccagtg tcggcgacat tttgaataaa catgtttttt acgtaaaaga
    27841 atccgcctat cttcgtgata ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc
    27901 ggtcgttgat gaacagaaca aagtcgtagg tatcctgact cgtgcatcat tagtggatat
    27961 cgtctacgat gtgatttggg gagaagatcc cgcaactact gatgttgcag agtcagctac
    28021 tcctgaagaa tctaaggagg tctgacctat gcagcagttt tttcatcagc acggttcaga
    28081 aattctctca aaaggttttg aacatcttta tatttcagga cttgctttga tcattggtat
    28141 catcattgct gtaccgcttg gtgtcttact gacacgtttt ccaaaagctg ccacgattgt
    28201 gattggctta accagtgcct tgcagacaat cccttctctt gctttattgg cattgatgat
    28261 cccgctattt ggcgtaggta aattacctgc gattatcgca ctctttattt attcattatt
    28321 gccgatttta cgtaatacgt acattgggat gaaaaatgtc gattggaatt atcgggatgt
    28381 ggcaaaaggc atggggatga ctgagttcca atccattttt tccgttgaat tgcccattgc
    28441 tatgccaacg atcatggctg gtattcgttt agctgccgtt tacgtgattg catgggctac
    28501 acttgcctct tatatcggcg ctggtggttt aggcgatttg atttttagtg gactgaataa
    28561 ttaccaacca gatctcattt tggcaggtac gatccctgta acaattttag cactattggc
    28621 tgattggctt ctaggtctct tggaacaccg gctgacaccg atcgcactta gagaggagaa
    28681 tgaagaatga aacgtatact aaaaatgacc atggttcttt tctctcttct tatacttgcg
    28741 agttgttcct tccctggtct tgccagcaat acagatgaag acaccatttc gatcacaggt
    28801 ggaatcactt cagaagcaca gatcttagca agtctagttg ccggaatggt tgaacattat
    28861 acagataaaa ataccgcaat catcaacaat ctggctacta caactatcaa ccatcaagcg
    28921 atgatgaacg gagatgcttc aatttcagcg gctcgctaca caggaacaga tttaacgact
    28981 acattgaacc ttcctcctga aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa
    29041 tttgagaaaa gatacggtca aacgtggttc ccgtcctatg gttttgaaaa tacgtatgtc
    29101 tttcttgttc gaaaagacac cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa
    29161 aatgtagcgg atgaattagt agctggtgta gatacttctt ggatcaatcg taaaggagac
    29221 ggttatgacg gcttccaaga aacttatggc ttctccttca attcgatatt acctatgcag
    29281 attggacttg tttatgacgc agtcgaagca ggaaaaatgg atattgttct tggctactct
    29341 acggatggac ggatcgccag ttatgatcta gtcatgttgg aagatgatcg tcaattcttc
    29401 ccaccatatg atgctgctcc agttgtagac aacaagatat tagctgatac gcctcattta
    29461 aaagaagcct taaataagtt aggtcatacc atcagtacgg aaaaaatgca ggaattgaac
    29521 tacgaagcag ataataatct agtggaacca tcagtagtag cacaacgttt cttacaagaa
    29581 aaccactatt ttgaagggaa gtgaagcaat tggataaaat gaatgtcttt caacaatttt
    29641 tctattattt ccaggaaaat ggctcctata tctttgcaca atttatccgt cacttcctga
    29701 tttctatcta tggtgtcctc tttgcagcag ttgtcgggat tccggttggg atcatgattt
    29761 ctaggagacg taaattagca aactgggtga ttcgtttggc gaatatcatc cagacgatcc
    29821 cttctctagc catgatctcc attttaatca ttggattagg cctgggagtc aatgtcgtga
    29881 ttgttactgt atttttgtat tccctcttac cgatcatcaa gaatacgtat accgggatga
    29941 tccaagtaga caaaaacatc cttgatgtcg gcaaaggcat ggggatgact gctagacaac
    30001 gactattcat ggtagagtta ccactctctg tttctgttat catggcaggc ataagaaacg
    30061 cgttagttgt tgccatcggg ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg
    30121 gagatatcat tatccgagga accaatgcaa cagagggcac atcgattatt ttagcaggtg
    30181 cattgccaac tgcattaatg gcgatcatca cggactggct tttaggtatt ttggaaagac
    30241 ggctagatcc agccagccgt acatcacgct gatgttattg taaaaaacaa aagcttctgc
    30301 gaccatttgt tggtcgcaaa ggctttttta gttctgtcat ttttaaggca ataatccact
    30361 ctgattcttc cctccttatg ggcaaagagt tgcttttctt agctatttat ccattccgta
    30421 tactccaact tcatactgcc aacgggttca tatctgcatc caccgactaa gtttttttct
    30481 tatccgctaa ggcattttgc caatccattt tcccatcact cgtcatggaa ttcataccac
    30541 atgatctcta aatactcgtc tgcttgttct ttgaattggg catcaattct tacatccttt
    30601 ggcgtggctt tcatttaagc cttttccatc agtcctcgat tgttcgatgc cttcagctgc
    30661 atcatcaaaa ctggtgactg ccgtatcggt aacttcgatc atcttttata caatcgggaa
    30721 cttgccatcc tcatccttgg ctaaaaacat atctagccat tccagcttct cgccatggga
    30781 ggtttgcggt tcctaaaacc ggtacacgat tcttatggac tgcatccgtc gcaactagac
    30841 ttggcgatac gatgatcctt caccagaaga gcacccccgt aaaccaattg atcgatatat
    30901 tgccaataag aaaagacatt ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg
    30961 atactgctat tcatggccga aagcgccacg actttaactt cttccgtttg agtgacattc
    31021 atgttcggca atgtcgcttt ggatacccgc tctgctaaag gaacgacact ggtattgtat
    31081 ttcgtatctg tatcttttcc aaaagaccat gccaacgaat cctctgggaa ccaataagac
    31141 gactccggtt gattgtccat tgcttttttc aacatacttt tggctgtttc tcgataaacg
    31201 acatttccaa ttgtcttcgc ctcactttgg cgataatcga tggtcgttgc cactgccaac
    31261 actaacaacg atgctccaat tttactgatt ttccttgtca cacttcttcc tccttgtttt
    31321 acataaaccc gtcaacattc cattcctaaa ctcaaacaat gacccttcaa aggtggagat
    31381 ataggaacaa attaaagaaa tcatgcaata aatccaagaa ggttggtttt agaacaaaaa
    31441 aagagtctcc tttttgggga gactctttgt taactatcta aaaaggttgg ctcttctcac
    31501 ttcactcata atgtgtccag gcagaataaa tttctacaac tttattttcc tcatcaactt
    31561 tgtaaaccac tcgatgctgt ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc
    31621 tcagcttttc aaaagactgc gtgggcatat atggatcttc cttcaatgtc tgaatcactt
    31681 cttcaaattg tttctttaaa ttcgtttgct tgatttttct taaatccact ttcgcagagt
    31741 ttttgattgc gactgtatag ttactcatca aagagcttcc caatcaatat catcgatatt
    31801 cgtaaagcta ctgtcgtctt tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc
    31861 aagaaaaagg gtttcttgaa tcgctctcca gtcttccagc ccaatcaata cagcattgtt
    31921 tccactacga tcactaataa tctcgatttc agtatgattt tcatttacct ctttcaataa
    31981 ctgataaaaa tttttacgag cagtgctagg attcatcgtt gtattagcca ttgtctctcc
    32041 tcctttttct gatataatta tagcgtactt gattaagtac gtcaaaaaaa tacaataaag
    32101 cacttaaatt cagcaaactt ttcctctttt ttacatagag aaaaagaccc ctttttaggg
    32161 aggatctttg tccacgctca cttcttactc ctttacgact agcggtagcc aaacgtaaag
    32221 taattggtaa caacgtatca tctttagcaa aggtcgaatc caaagcctgt gagtctatgc
    32281 aacaacacca atacacgaag gttcctcgtt gcagtgtgac ttgattgata tcttgaataa
    32341 cctgaggata cgcacgaaag atttgtaacg gttattcaaa actgattttg gccgtcaagt
    32401 cttctactgc ctcaaaaatg attgcctctt tcgtctcctg tcattcatgt tcactatcga
    32461 tttgatactt gcttagaccg ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta
    32521 tggtaacaaa aaatggtcac ttgacgaggc tgagattcct acttatgaag acacatggca
    32581 cttaagagtc gaggtaaagg aagaagctga aaatatagga ggctgtacat tatttggcaa
    32641 aggattcgga aattatgatc aagacatcaa aatcaaaatg ttttacagct aaataggaag
    32701 aataataaca tttcatacgc tgttcccatc tttaatggct tatagagggc acctttagct
    32761 gagaataaga caaaacagac cctctagctt caattaagag ctagagggtc tgtttttgat
    32821 tttaaaactt ggtttatttc ttcaaatggt agctataggt gctgacaacg atattttcac
    32881 gccaagacaa tcgcaatctt aaccgcaaac ttgtttggtt atggagaata ttctgccagc
    32941 gtctgtttgg tacaaattga ggaatcaaca cagtagttgt atagttttgt ttcgctgcat
    33001 ttttacttac gatatctaca taacggataa ttggattttc aatcgagcga taagaagagt
    33061 ggacgattga aaaacggaca tctgggaaat gctttttaaa ttctgcttcg atttcttttt
    33121 ctttttcgac atcttcgtct agcgaaacat gcatcgcaac gacataatca ccgatcgaac
    33181 gtgcgtaatt caacgcaccg atatttaccc gtgtcacatt cccgactaat acaagaacgg
    33241 tattcccgtc ataatcatgt agttttgcat cattttctaa acgaagctgt tctgctactt
    33301 ttttataatg atcatggatc ttataaaaaa tgaacataac gattggcata atgatgaaga
    33361 aaggccaaat gtcccctaac cgatagacaa acaaaatcgc aatgattgca tatgagatga
    33421 acgcacccgt aatattggca atcgatttgc ttaaccatct ttttccttct tttttccatt
    33481 tgacgaccat ccctgtttga gataaagcaa atggaatgaa cacgccgata gagtataacg
    33541 gaatcaagcg ttctgttgag ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta
    33601 gtgtgataat cccattggaa tagcctagtc gatccccacg atcttgatac atgtgaggca
    33661 tgaatttatc ttttgccaga ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag
    33721 cagcaactgc taaaatcagc gctgtagcaa attgaaggac gtaatacaaa ataccatgtc
    33781 caaagacagc tttaccaatt tgagacaaaa cagtgacttc tttttcagga acgatcccat
    33841 accaatagtt gataaatgtg atccccacga agaagaaacc taagatcaat gccatcatcg
    33901 tcaatgtagc agcggcattt tttgctcttg gcttcttaaa gaatggtaca gcattactaa
    33961 tcgcctccac cccagttaaa gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga
    34021 tagaaacacc cggcacagcc gctcctggaa gtgcagtagc ttgcaatggc acagcacctg
    34081 tcacgatatt atataaacca acaacgatca gtaacgagat cacaaggata aatgtgtaca
    34141 ccggaaataa taggaacgat gcagattccc tcagcccacg aagattcagc atcatcagta
    34201 gcaaaacgat tgtcacagaa atagcgactt gatgaccgta gagtgctggc accgctgatg
    34261 tgatcgcttc agcacctgct gagacagata ctgctactgt caacatgtag tcgatcaata
    34321 aagagccacc ggaaatcaag ccagcattct ttccaagatt ctcactgctg acgacatatg
    34381 ctcccccgcc atgaggataa gcatggatga tttgacgata tgatagcgtt aatgaaatca
    34441 ataaaataat aacaaaagcg gcaatgggta gcgaatacca gatggctgca gcagacaaag
    34501 cgactaaaac gacaacgatt tgttccgtac catatgcaat agatgataac gcatcagacg
    34561 acaataatgc caaagcagca aatcgggtta atttgtgctc atcattttca gcagatttta
    34621 aaggcttacc tactaacaac ctttttaagt aatccaattt ctaaactcct ttacacacaa
    34681 ttttacttca agttttatag tcgaaaattt atagtaaaca agatgttata gtacgctcac
    34741 tgaccttaaa agtcaataaa ctttaagaaa ataattggaa aaaaacagaa aaatatcaag
    34801 cgctttttca aacgctttaa tacagcaaaa atctaacatt tttttatggg ctttttcttt
    34861 gaattctata ataaaaggat agaacttgac ttagaaaatc aagttctatc ctttttgata
    34921 tcttaacttt tggaagagag atgtaaggct gctttctacc catcattctt actactcgaa
    34981 gcatagcgca gttgtcacaa cctcttgata aacggtgttt aaaagcttgc atctgcggta
    35041 ataagcccaa ccaaattaaa aacataagaa gctgttttta tttggtcgtt cttattactt
    35101 gatgcagagc agctttttta caacctcttg gtacacggtg ttcaatcagc tgaccctcgg
    35161 tattagctca aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
    35221 tcaggtcaaa acactgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
    35281 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
    35341 ttccaaagac aacaaacaaa ccgatcatga tccagattgc tttcgtagca tttggccaat
    35401 aaattcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
    35461 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    35521 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    35581 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    35641 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    35701 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    35761 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    35821 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    35881 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    35941 ttgttgcgtc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    36001 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    36061 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    36121 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    36181 ccaatcctaa tttttgctga atgatcggta attcattgtt gtataggtct gccccttcat
    36241 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    36301 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    36361 cagttgcttt cgttgagctg agaagcgaat caaagtcgat ctggtccgct ttgctcagtg
    36421 cgccttgtgc agtcccaatc gtatcgatga cttggtctag tagagaagtg acagtggaac
    36481 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    36541 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    36601 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    36661 ttggctgtaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac ctcgaccagt
    36721 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    36781 tgtttttctt ttcatcatca gtcagcttac caacttcaat agctgttctt acagtatcta
    36841 ttaacgaacc tgtcgttgtt gccacttgtc caattgactt caatgtaaca tctacactgt
    36901 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    36961 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcttgt acttgttgga
    37021 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    37081 caaaatcgcc gtcgatctct gctagttgct taccggcatt ttggatctct gggatttttt
    37141 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    37201 caacgacttt ttcacccatc tcatctactt tagggatgta atccacgaat tcatttgctt
    37261 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    37321 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatcttta actttattga
    37381 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    37441 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    37501 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    37561 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    37621 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    37681 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    37741 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    37801 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    37861 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    37921 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    37981 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    38041 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    38101 ataattaagg attacagcct ttactttatc atgaaataaa agggaaagat aattttatgc
    38161 caaagttttt atctattata cgtatacgca aaaaagctaa aacatttttt gtctttgccc
    38221 ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt atttcttatt tttcgaatga
    38281 caggatgatt ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt ggatgacatg
    38341 cttaagtgaa gcagacagat tcattgaaat aaaaacatga aaggctcctt tttggtaggc
    38401 tttgattatg ccagcaaagg cttctgcatc tagatattcc tttcttggaa caaacgattc
    38461 ataaaagcct tccttttttt gccgcaaatc tagatctggg aggtcattta atgcaacata
    38521 cctgccgcct ggtttcatga tctggatgcc ggtttctcct ttgatacttc ctttcgtcgc
    38581 atcgattaca aggtctgcct gatcagcaaa ttgcagacct ggatttgttt tatcataagc
    38641 agcaaatgct gaagctccta atttttttac cttttcctca tttttactgg atgcactggt
    38701 taagattcgg attccctttt catggagaag ctggatcaga cttgacccga ccgcacctga
    38761 tgctcctagt atcattacag tatctgtcgg ctggatttcc gtcaaatggt tgattagatt
    38821 ataagccgta attcctggcg tgaccattcc ggctgcttct tcccaactca ttttatctgg
    38881 aatttttgct acttttgctg atggcaacac gacttcttct ccgtaagtcc cgccgactgc
    38941 atggacagct actcgatcac ctacatgaac agttgtgaca tctgaaccaa tctctgtcac
    39001 aataccagcg ccatcattac ctggtacata agggaatttc aatgttcgta tctctttcat
    39061 ttctcctaaa cgcaaggcaa catcataagg attcacactg aatgctttta tttctacacg
    39121 aacatgtcca gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa catcttgtgc
    39181 ttctccgtaa ccgttaattg caaatcttcg catggtcacc atctcccttt ttctttaagt
    39241 ttacgtttat gcctttattt tgacaaaaaa aatgtctttt cgttttggcg catgattcaa
    39301 aacaaaatag ttgtcttttt tcgcgtaacg tgttaaatta tttatctaaa agcgtaaaga
    39361 ttacgattta cgctaataaa aaataattgg aggaatttgt tgatggctaa aaaatcaaag
    39421 attgccaaag caaaaaaaca gcaagaaacg attgaacgct atgctgcttt acgccaggaa
    39481 ttaaaagcgc aaaaagatta cgaaggcttg cgtaaacttc ctttagatgc tcgtccagaa
    39541 cgattgaaaa atcgtgattt aatcgatgga cgccctcgcg gatacatgcg taaattcggg
    39601 atgtcccgta taaaattccg tgaattagca cataaaggtt tgattccagg agtaaaaaaa
    39661 gccagctggt aatccaagtc tggctgtaat gtaaaggagg agggcgcatg gaccactcct
    39721 tggatcatca aaaaa
//
LOCUS       DACEOM010000019        39133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEOM010000019 DACEOM010000000
VERSION     DACEOM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.020"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..221)
                     /locus_tag="IW781_000949"
     CDS             complement(<1..221)
                     /locus_tag="IW781_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1181964.1"
                     /translation="MKPNPVETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERG
                     FSLPNKERLIKIAELSNKTIDWIKWGTLE"
     gene            527..715
                     /locus_tag="IW781_000950"
     CDS             527..715
                     /locus_tag="IW781_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ1181965.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            761..1138
                     /locus_tag="IW781_000951"
     CDS             761..1138
                     /locus_tag="IW781_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ1181966.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(1698..2870)
                     /locus_tag="IW781_000952"
     CDS             complement(1698..2870)
                     /locus_tag="IW781_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181967.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKALNMVYKQLMFE
                     ELIENSIKELDHEYIKRELSLEERIDNLLSNEVIDQKKLDKTKRELSKLRIAKNKEAR
                     DVLTQAIGLKSQTRIRDIAKKLDFDYSDFIDSAKMKASKDFSNDFVGIVTGQRTPRIY
                     RNKNKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGRKPRKARDSALTLERIMDG
                     QYKLNQSNLIFEGKNLFLNLTISFEKDYHKLDFKEGAELTIKFTDLCSGKCLVNNQSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIIQTAIRNKTQIICISEPDKNYGEWAYYQLKEQIINKGKRYGIEVKSE"
     gene            complement(3114..4082)
                     /locus_tag="IW781_000953"
     CDS             complement(3114..4082)
                     /locus_tag="IW781_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1181968.1"
                     /translation="MSLENEKPYYVVVGGVNGAGKSTIYQVQNLKRKELDTDRINADE
                     ILRSFGGNWKKQADINKSMREVLREIKGHFKEGKSFHHETTFGGNPYPDKMRLLKAKK
                     LGYESFLIYIGLDSPERAIERVKIRVAKGGHGVPEDLIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYAWVPKAIQEKNAETPISKQIMMDISQEEM
                     NPKETLERIYSASDDFISDRFSKEAQVDDFYRNHKNEIKERILSISFSTGVSQDELVG
                     QDKRRNSCKLAYINSVGELLVKTDSELLSMELNRDRNIQKTDFEVE"
     gene            complement(4079..4276)
                     /locus_tag="IW781_000954"
     CDS             complement(4079..4276)
                     /locus_tag="IW781_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181969.1"
                     /translation="MRAMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLT
                     LSSFVTRVDQKKVINQKDFKI"
     gene            complement(4287..4604)
                     /locus_tag="IW781_000955"
     CDS             complement(4287..4604)
                     /locus_tag="IW781_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181970.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRFIEVNNLGIIEDYLDKLDEAIKQSIEKNGEDHILTVVLKDLKDRLEAEAEYF
                     NKD"
     gene            complement(4641..4859)
                     /locus_tag="IW781_000956"
     CDS             complement(4641..4859)
                     /locus_tag="IW781_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181971.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKAIGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            complement(4968..5159)
                     /locus_tag="IW781_000957"
     CDS             complement(4968..5159)
                     /locus_tag="IW781_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181972.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            complement(5321..5677)
                     /locus_tag="IW781_000958"
     CDS             complement(5321..5677)
                     /locus_tag="IW781_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181973.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            complement(5702..6706)
                     /locus_tag="IW781_000959"
     CDS             complement(5702..6706)
                     /locus_tag="IW781_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ1181974.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPVSVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            complement(6743..7003)
                     /locus_tag="IW781_000960"
     CDS             complement(6743..7003)
                     /locus_tag="IW781_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181975.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            complement(7012..8085)
                     /locus_tag="IW781_000961"
     CDS             complement(7012..8085)
                     /locus_tag="IW781_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181976.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            complement(8093..9919)
                     /locus_tag="IW781_000962"
     CDS             complement(8093..9919)
                     /locus_tag="IW781_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1181977.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATNEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSTPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKHDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            complement(10074..10940)
                     /locus_tag="IW781_000963"
     CDS             complement(10074..10940)
                     /locus_tag="IW781_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181978.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWKMVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYESTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            complement(11115..11378)
                     /locus_tag="IW781_000964"
     CDS             complement(11115..11378)
                     /locus_tag="IW781_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181979.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKTALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            complement(11380..11709)
                     /locus_tag="IW781_000965"
     CDS             complement(11380..11709)
                     /locus_tag="IW781_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181980.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            complement(11731..13266)
                     /locus_tag="IW781_000966"
     CDS             complement(11731..13266)
                     /locus_tag="IW781_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181981.1"
                     /translation="MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            complement(13333..13800)
                     /locus_tag="IW781_000967"
     CDS             complement(13333..13800)
                     /locus_tag="IW781_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181982.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKSNMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEQESSRLGEGTMFSSPSLRFTTD"
     gene            complement(13797..14093)
                     /locus_tag="IW781_000968"
     CDS             complement(13797..14093)
                     /locus_tag="IW781_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181983.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVNKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            complement(14444..14674)
                     /locus_tag="IW781_000969"
     CDS             complement(14444..14674)
                     /locus_tag="IW781_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181984.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            complement(14710..15606)
                     /locus_tag="IW781_000970"
     CDS             complement(14710..15606)
                     /locus_tag="IW781_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181985.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMIIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYQNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            complement(15620..16270)
                     /locus_tag="IW781_000971"
     CDS             complement(15620..16270)
                     /locus_tag="IW781_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1181986.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATPSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            complement(16285..17421)
                     /locus_tag="IW781_000972"
     CDS             complement(16285..17421)
                     /locus_tag="IW781_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181987.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            complement(17443..17817)
                     /locus_tag="IW781_000973"
                     /pseudo
     CDS             complement(17443..17817)
                     /locus_tag="IW781_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
     gene            complement(17951..19828)
                     /gene="ltrA"
                     /locus_tag="IW781_000974"
     CDS             complement(17951..19828)
                     /gene="ltrA"
                     /locus_tag="IW781_000974"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1181988.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            complement(20516..22210)
                     /locus_tag="IW781_000975"
     CDS             complement(20516..22210)
                     /locus_tag="IW781_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1181989.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIKNLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            complement(22373..22966)
                     /locus_tag="IW781_000976"
     CDS             complement(22373..22966)
                     /locus_tag="IW781_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181990.1"
                     /translation="MRVKGQNSVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFIIEGRTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(22967..23299)
                     /locus_tag="IW781_000977"
     CDS             complement(22967..23299)
                     /locus_tag="IW781_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181991.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(23351..25723)
                     /locus_tag="IW781_000978"
     CDS             complement(23351..25723)
                     /locus_tag="IW781_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181992.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKIGLPKAQNLPMKDSRLNQ
                     FKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAP
                     EPIQRVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP"
     gene            complement(25720..28329)
                     /locus_tag="IW781_000979"
     CDS             complement(25720..28329)
                     /locus_tag="IW781_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1181993.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            complement(28329..30392)
                     /locus_tag="IW781_000980"
     CDS             complement(28329..30392)
                     /locus_tag="IW781_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181994.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            complement(30462..30764)
                     /locus_tag="IW781_000981"
     CDS             complement(30462..30764)
                     /locus_tag="IW781_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181995.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            complement(30782..31024)
                     /locus_tag="IW781_000982"
     CDS             complement(30782..31024)
                     /locus_tag="IW781_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181996.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(31021..31485)
                     /locus_tag="IW781_000983"
     CDS             complement(31021..31485)
                     /locus_tag="IW781_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181997.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            complement(31512..33602)
                     /locus_tag="IW781_000984"
     CDS             complement(31512..33602)
                     /locus_tag="IW781_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1181998.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            complement(33599..33859)
                     /locus_tag="IW781_000985"
     CDS             complement(33599..33859)
                     /locus_tag="IW781_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1181999.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(33872..34627)
                     /locus_tag="IW781_000986"
     CDS             complement(33872..34627)
                     /locus_tag="IW781_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182000.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(34643..35395)
                     /locus_tag="IW781_000987"
     CDS             complement(34643..35395)
                     /locus_tag="IW781_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1182001.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(35410..37386)
                     /locus_tag="IW781_000988"
     CDS             complement(35410..37386)
                     /locus_tag="IW781_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1182002.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(37439..38110)
                     /locus_tag="IW781_000989"
     CDS             complement(37439..38110)
                     /locus_tag="IW781_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1182003.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(38196..38390)
                     /locus_tag="IW781_000990"
     CDS             complement(38196..38390)
                     /locus_tag="IW781_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182004.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            complement(38502..38741)
                     /locus_tag="IW781_000991"
     CDS             complement(38502..38741)
                     /locus_tag="IW781_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182005.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(38855..38983)
                     /locus_tag="IW781_000992"
                     /pseudo
     CDS             complement(38855..38983)
                     /locus_tag="IW781_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
BASE COUNT        10824 a         7810 c         5469 g        15030 t
ORIGIN      
        1 tcttctaaag ttccccattt aatccagtcg atagttttgt tacttaattc agcaatttta
       61 attaatcttt ctttgttggg aagggagaat cctcgttccc aattattaat atttcctttt
      121 tgagcatttc caattaaatc tgcaaattgt tccatagtca ttccaaaatt agaacgaatt
      181 gatttaattc ttttacctgt ttcaacagga tttggtttca tacaatcacc tcttttacag
      241 aatttggttt catagtatct tattcgctct ttgagttcta agttctcttt ctccaatttt
      301 tctatataat attcttgatc ctgtttcttg ttcaaaaaat gacctccaaa tttcacattg
      361 tgttacaccc tagtgttaca cctccaaacc ttattatact gtactttacc atatgataca
      421 atagggtata ccctattgtg acacttacat tctactttta atgtaaaaac tcttgacctt
      481 tatacgcata atgcgtataa taatatttgt aaggaggtag tcgcgaatgc cacttaccgg
      541 taaaaaaatg ctcaaactag ccaagaaaaa tggttggaaa gtggtgagga taactggcag
      601 ccatcatatt gtagccaaag aaggattccc acccgttcct attcctgttc atagtaatca
      661 ggatttaaaa aaagggacag agcagaagtt actaaaggac ttagggctga aatagcccag
      721 tcctttagtt cattggatta actcgaaagg agtggtggaa atgaaaaaaa gttaccctgt
      781 aattttttct gctgaagaaa atggctactc agtagttttt cctgattttg ctggagcaac
      841 acaaggagat aattttgaag aagctatgtc caatgctaaa gaatttcttg atgggattct
      901 agcttactat attgatgaag atatttcagt tccttctcca acagatattc atcaactaaa
      961 taatttgggc aataacaaaa ttgtttctct tattcaaagt gatccttctc catttatcca
     1021 aaaaaagacc gtacgcaaaa acgtcacagt cccagaatgg ttagtagcta gaggggaaaa
     1081 agataaaata aatttttcta aagttttgac agatgcgtta ctaaaaatat acgagtaaac
     1141 gtaaatatat taataacaat ttagttgcct aatgacatag atagcggaat taaaagtaaa
     1201 atagtactca tttattgcag gttcccttag aatcaataca ataaatgagt actatttaga
     1261 ctgtttaact actagtaaga tatagcgatc atcagtttca gggttatccc ttggtgacca
     1321 tagttttgcg tatttttcaa ctcgttgcca aatcaacgat tctatttttt tatttctact
     1381 taactccaaa acggagtacc ttctttttct tcgttagctc ctatttaaga gccatgtcgt
     1441 tttatataac tctaaaacag aaactactga tcaattcaat aaagccttat ttgagaactg
     1501 tgttgtttta catggtttta aaacctgatt tcccgcaaac ggtttatccg taacatttga
     1561 gaactgtgtt gttttacatg gttctaaaac ctcaacaata ttcgtccttc cgtctggtta
     1621 cacaacttga cagagctaac ccttcagtga gtgagtgtcc cctcactatt tcgttgccaa
     1681 atcaacgatt tcttattcta ctcactttta acttctattc catatctttt ccctttattt
     1741 attatttgtt ctttcaactg ataataagcc cattccccat aatttttatc tggttcagaa
     1801 atacagatta tttgcgtctt attgcggata gctgtttgaa taatttttgc cgaacattga
     1861 ttattatagg ttttaacaaa attgctttct cttttcttta tggcttccca tctatcttga
     1921 tctaattttc tttttcgacc atgaccgcct ttagaaaaaa ctcctcggct ttgctccctt
     1981 gatttcattg ctttaatttt atttttgaag ttacttatat atttgctgtc accaaaatca
     2041 atcacagatt gattgttaac taaacattta ccgctacaca aatcagtaaa ctttatggtt
     2101 aattcagctc cttctttgaa atctagctta tgataatctt tttcaaaaga aattgttaaa
     2161 ttcaaaaata aatttttccc ctcaaagatt agattactct ggtttaactt gtattgaccg
     2221 tccataatgc gttctaaagt taaagcgcta tctctggctt ttcttggttt tctacctaaa
     2281 tttactttaa acctaaatcc tttaggtaaa gagatataaa acttgtcagc tttgtgaatc
     2341 ttaatatccc gacctctgaa cggtaacaat ccatatttat ttttatttct atagattcta
     2401 ggtgttcttt gcccagtaac aattccaaca aaatcattgc taaaatcctt actagctttc
     2461 attttagctg aatctataaa atccgaataa tcaaagtcca atttttttgc tatatcacgt
     2521 attctggttt gactttttaa acctatagct tgtgtcaaaa cgtctctggc ttctttgttt
     2581 ttcgcaattc gtagtttaga taattccctt ttcgttttat ctaatttctt ttgatcgatc
     2641 acttcattac ttagtaaatt atcaattcgc tcttcaagac ttagctctct tttaatatac
     2701 tcatgatcca attcttttat agaattttcg atcaattctt caaacatcag ctgtttatat
     2761 accatattca gcgcttttac atattctcta ctatttttcc aaaaaaaacc aaaaaacgaa
     2821 tctctttcct cttgtgatcc atcaacatat acttctattt ttttagtcat agttattatt
     2881 gtcaatttta gcacctcatt attatatatc tggtatataa taatagtaac atatttccta
     2941 gactataatc aatgatcaat tgtctacttg tagtaattat attaaaaaca aaaactaaaa
     3001 ataatatatt attccttctg actacatata gtcactttat ccaaaaaata agaagtgctt
     3061 tcgcactcct tattaaattt atttactatt aattgattca gctacaactt cttttattca
     3121 acttcaaaat cagttttttg gatatttcta tctctattta attccatact taatagctca
     3181 ctatcagttt ttactaaaag ctcacctaca ctattaatat atgccaattt acaagaattt
     3241 cttcttttat cttgacctac taattcatct tgagataccc ctgtagaaaa actaattgat
     3301 agaatccgtt ccttgatttc atttttatgg ttcctataaa aatcatcgac ttgcgcttct
     3361 tttgaaaacc gatcactaat aaaatcatca cttgctgaat atattctttc aagggtttct
     3421 tttggattca tctcttcttg agatatatcc atcataattt gtttgcttat aggagtttca
     3481 gcatttttct cttgaatagc tttgggaacc caagcatatt cttgcgtatt attggctaca
     3541 actgtatgat tttctttaac ataaatataa gaaaactcat ctgaattatc atagatactt
     3601 acattatcaa aatatttctc taactctgct aagtttctta aagaaaattc gtatcttttt
     3661 cttattaaat cttcaggtac accgtggcca cctttagcaa cacggatttt aaccctttca
     3721 atcgctcttt ctggtgaatc tagtcctata tagattaaaa acgattcata acctaatttt
     3781 ttagccttta gaagtctcat tttatcggga tatggattac caccaaaagt cgtttcatgg
     3841 tgaaaagatt ttccttcttt aaaatgacct ttgatctctc ttagaacttc acgcatactt
     3901 ttgtttatgt ctgcttgttt tttccaattt ccgccaaaac ttctcaaaat ttcatctgca
     3961 ttaattctat cagtatcaag ttcttttctt tttaaatttt gaacttgata aatagtagac
     4021 ttaccggcgc cgttaacgcc cccaacaact acataatacg gtttttcatt ttctaaactc
     4081 atattttaaa gtctttctga ttaattactt ttttttgatc tactcgtgtc acaaaactag
     4141 aaagtgtaag gtagaaagtc ctttcttcct cactttctgc attttttaag gcttcatgaa
     4201 aatcatctac agtcatattc aaaacatcag gatacatttt ctttaattct tttacagcct
     4261 ctgtcatagc tctcattccc ttcttattag tctttattaa agtactctgc ttcagcttct
     4321 agtctatctt tcaagtcttt taaaacaacc gtaagtatat gatcttcacc atttttttca
     4381 attgattgct ttatcgcttc atctaactta tctaaatagt cttctataat tcctaaattg
     4441 tttacttcga taaatcggaa gtccggtaaa tttttcatcc gaataccgcc tttaggctgt
     4501 tcctttacaa ataaagattt gtaatcatta tattcattcc cattaataac caattctttc
     4561 ataggctcta agtggataaa tttacgtgaa gcatagtctt tcataataat ctctcccagt
     4621 agaaaatctt cttttttcta ttatatcacg ttctcaaagc gttgagttat tggattgttt
     4681 aaaccatcaa gacacatcat gctgcatttt ttcatcacta ccggtgcttt tccgattgct
     4741 ttaactaccg ctacattagc taacaaacta attacttctc cctcgataag ctctccgctc
     4801 ccaataacaa cgccctgtac ttccatacct actttaaacg tttctttttt atttaccatt
     4861 attccgctct ccttccatga ctacttgtag tcatttggat attttttagg ggaacggttt
     4921 tccgttccct ttcatcaaat agaatccttc atcatttcaa caaataatta actttcagtg
     4981 taaaaaaatt cttcgtaatt accatcatga aaaatatccc atctttgttc gtcattctca
     5041 ctgaagtagg taaaataata tctgacagta tctaacttat ttaattgctc tctggttttg
     5101 taaatacaat ccaagtgatc ttgcattcga tctgaccaaa acattcttgt ctgctccatc
     5161 tattatctct cctttttatt aaatcaaggg tttgagtctt tgactacttg tagtctatgg
     5221 ctatatcata caggaatgac tacttgtagt caatggtttt ctttagttta attaactgtc
     5281 tattcgtagc caaataatgt aaaaaaacgc ctagtttaca ctagacgttt aaacaattca
     5341 ataattcttc gttatacccc attaagggtt gaactgcttt aatacttact tgatcgtctt
     5401 ctctaacggc ttctatgctt accccttttg tatattggcc atctttctta tccgtccaaa
     5461 tttgataata agtaagccca tatcgttcaa taatctcaaa gagaatagtt attaattttt
     5521 tttgtttaaa ttttggtaat tcaatacgca atgatttttc ttgaatatcg aactctggat
     5581 ttttattttt aagttgttct tcaataatca tatagtctgt agacgtctta tctaaatcaa
     5641 ttaatgaatc tgataagatt gctattgtat cgttcataac acatcaacta ctttcttctt
     5701 tttattccat ttcatataca ggagtattaa gagataattt atgtgtgaag ttcacgattt
     5761 ttgatacatc ttctaacttg aagcgtttaa aagcttcttt tcctcttgtt aaattatcat
     5821 tccaatcaaa tccctctcgc aaaggtacat cgacttttac ttcactgatt cctatccctt
     5881 ctcttccaat tcctttttct ttaaattcag tttcaataaa agtacgtccg gctttatcat
     5941 tatcaacaca aaggactgtt tctgaaggca atgatttaaa cttattataa tgctctgtaa
     6001 ccccatgcaa tacattacct acctttaatc cattcatgct aacaaacgta gcattttcgg
     6061 ctttttctgg cttttgtgta atgtacgaca tcaaatctat ttcactttca aaaaaataca
     6121 ttttatctgg ttgaccaaaa gttacgttaa atcctgaata ctctttagag ttttcttgta
     6181 tttttttcca tgtaacatcc ttttgtttgc cttcatgaaa aaattttcta gttccttgtt
     6241 ccgtacaccc aacgatttct tgatcttttt tccataagaa caaaacattt ccttttttat
     6301 cttgtttaat tagtccttta gataaaaaat cttccactaa agctgtatcg attcttctct
     6361 catttactaa ataagacttt gcaacagata ccggcacttc attttctttc tgatattggt
     6421 acggagcttt tttcactttt gtattgaacg aatgaacttc ataatcacta tcttcaagtt
     6481 tctgttttgc ttctttaaat gaacaatcgt agtaggcttt gataaaatca aaaatatctc
     6541 cccctaaatc ctttgagttc catttaaaaa ggtttttacg atcactgatt acacaagaat
     6601 catgatcttc taatctatag tattcccttg aatcttgctt taaacctatc ccattttgtt
     6661 tgcaaaaatc aacgatactt actttttttg cctttttcaa atccaaacaa aaaccccctt
     6721 tactagacca tcactagtaa aattacgata aggtacactt gtatttttca taataatcct
     6781 ctgtacagaa aataaagttt agcccatccc taagagataa ctcccaaaag cctatcttct
     6841 tgctcttttt ttctctaatt atcttttttc taatactttt tccgtacttc aaagccattt
     6901 taaaagcgat ttgtacatct tcacaaatta caaaatgact attattaatt tgacgatatt
     6961 caccttgcgt ttctaaaaaa tttctatttt cttgttctat cattcattta tctactccat
     7021 ttcaaatttt gttgtatttt caaaattagt caatttcaaa atcttattct ccatttcttc
     7081 agaaacaggt ggtatttcaa acgtatcatt ttcaatttca ttataaattt ctctcattgc
     7141 accgttgtaa gccatgatag acgtagcttt tttgtattcc gtagaagaag tatctactgg
     7201 aaaacctagc agttcttctt tatcgatctc taacttttcg gataaatttg tcaatttcgt
     7261 ttcaatttct tctttatgac tatcataaaa ttcattaata tcactatcta atgctaatct
     7321 ggaaactggt tcttccatgt tttctaagta ttcaatgcgc tcttgccaag cttgtttgtc
     7381 ataataatta gttgtttcta gctcttgctc aaaatctttt atcacaactt ctcgaatggg
     7441 aacttccgaa accttatgcc caacaaattt atctgtcatg tcctcactac tttctaagct
     7501 attccgttcc tcgtttaaat cgtgaatagt ttcaatcttt actttatcag gctgtaacac
     7561 tacgataact acatcctttt gtcttaactg ttcttccgca ataatatgtg tatatttccc
     7621 aacgtcataa aatgccttag cagtagcttc atgatctcca attgtatttc caatgtcgtt
     7681 aatcaaatct acgtcatttt cattctcact aagagaagca atcacttctc tttttacaac
     7741 tttttctccg taataactaa catcattgta atcatttaaa atatcattac tttcttgtaa
     7801 tttatctatt actttagaaa gctcttcatc agtaatagtg cgttcattca tagaaatcgg
     7861 cttctctaat tcaacattta cttcatatac gaaaccatta tttccgttac taccctgttc
     7921 agcgtacatt gtagccattt ctttattagg tgttaagtac acaccaaatc cattagctgt
     7981 tccgtttaaa cctttatgat ctaaactaaa ttcatcaaat ttattcatac ttccatgaaa
     8041 caggcgataa gttccgcctg tttcataatt ttgttcatta ctcataattt tttcactcca
     8101 tttcaaaaga acgttctgct gtttttacag tcggcttttc caagttagaa ttttctggta
     8161 tcttgaaagt tccttcatcc atttcatatt ttactttagt tgcaaccagt ttataagcta
     8221 ccactgaagc cacctcttta tatgactcat tgtctattgt atctcttaaa tttgttcctg
     8281 acacagattc aaaatctgta atcgtatttt cgatttcttg tttatgagat tcgtaaaaat
     8341 cttccgcagt catacaagag tttaattctt ttacatcatt aatatttgtt tggttttcaa
     8401 tttcttcttg ccagcatttt tcattatagt aattgttagc attttcagat agattataaa
     8461 ccgcttcatt tataacaact gatcttagcg gttcgcttaa ttttaacgta tcatttttta
     8521 gttcatgatt ctcgcaagct ttcaaacttt ctacaacaaa cttttcaaca taatcacaat
     8581 tttcaggtga tcgatctaat agtaaacgat catgtttatt ttcacttgga ttctcaaaaa
     8641 ctacaccata aacatcacat acatcaaatt gtttcgtatc tgaataatat gtcacatagt
     8701 tttctcctat tttagctgta acatcataat tgctatgagt aagctcaata gaattttctc
     8761 catactttcc tctataataa tccaccatcg cttctaaata actaccgtgg attgttctat
     8821 ccggatcgtt gattgtacta tcgtagaaat tatgaaattc taaatctgat ttttgttgta
     8881 tctgctcttt attctcatta ggaacaaagg aattttttag tgcattaaat gctgtttgct
     8941 gttcttccaa gactttctta tcaatcgtac ttataaagtc aatagacgtt tctctaactt
     9001 cttgcattat ctgtctaaag ttttttggat caattttttc attattttta gtccaatttg
     9061 caatataagg aatagtaaag tctgacgtat caataccgta attgtgacaa acaataaatg
     9121 aagtcatttc cgcttgaaac tctttaatcg gtgttgagta ggaagaaccg tgttcattct
     9181 tattgtgaag ctttttgtga gccaattcgt gaatactggt agtaagattt tgtgttctgc
     9241 tatttcttga atttaataca atttcttccc taccatcaac atattgaata tattcacctc
     9301 tagcgcttcc catttcacct ctaccagact cgttcatatc ttttatagat atattcatag
     9361 attcggccaa atgatttaca cctttttcta attgactaac acttaattca ccatcaatat
     9421 taaaattcca taccctgtta ggaaatattt ttggcaaatc agcttcagtt gcattagttt
     9481 gggaaatatc aaatacgtaa cccttcttaa aatgcttgat ctttttgctt cttaattctc
     9541 catttttgat tttttctttt tgctcgtttg tcgcttcttt taacgtcttt tcctcaccat
     9601 tttcatcaat aaaaaccgtt accggagcat gcgcataaat gcctatccct ttttctttcg
     9661 ctttaacttg aaatcctagt tttttccaac catcataact tgctacagca attgctcctt
     9721 caaatccctc atcgatcaac gataggttta aaggactgta attgtgtatt ttagacatga
     9781 acttggcaaa ctcgatcata tcttcagggt tagactgata ttgctcgatt ttgctgttgg
     9841 aagccttaat taaatcgtct agctcttttt gtttttgctc tttcgttttc ggcttccagt
     9901 ttcctttttt ataggccatc ttctatctcc tttctttttt aggtaagtta aattacctaa
     9961 ttaaattata ccagatatat aaaaactctt ctatctaatt tcactgactt aatgcaaata
    10021 aaaaagagat aagatagtta tagtaaccat cttatccctt cttattttac catctactca
    10081 actatactta cgtttttacc tttactatct aattgaactt tgttatcttt agaagctact
    10141 ttagtaagca aaagttgatc caattcatca ttagaatatc ctgaataaaa attatctaaa
    10201 actttcgttg tggcaactgg aattgataac ttcaaatcca tactagtaga ttcataagat
    10261 aattctcctt cagacgaaac ttcaccaaaa ctatttaaat tccaaattgg actatctaaa
    10321 tatttataag ataatttaac ggtttccatt gaaggtgatt tgttttcatt atccatttta
    10381 attgttgctg ataccatttt ccactccggc tctgaatatt gaacaccatc gatctcttct
    10441 atatccgcct gtattttagg tatactatct tgaaaatcag caattttttg gtcattttga
    10501 aactcttgtt tttcctcatt ttctaattct gtttttacag aatctaaagt ggcattatag
    10561 ctatcaatat cgcttttttt cttatcatta aacttttgtt tatcagcgtt caatgcttgt
    10621 tctattattt cttcatcact cattttagaa actttttcca atgtaagctg ttccatatcc
    10681 gttttaaata cttttgcttt gccatcttta gtttggatca ccgcttttac atattcattt
    10741 ttatcaatag gcgttttatt tttactactt gtttcgacta ccaaccaaca acgtgagtct
    10801 ttttgttctg cactatctat gaatgagata ttatgtgctt catttgactt agcaccagct
    10861 tcttctttgc cacatgcaga taataatagc gcaaaactaa aaatcatagg tattgttgca
    10921 ataactctta actttttcat ttataagcac ctctttttta tcggtctgat atataatttt
    10981 atattatcat atttttattc caatacacag atactcttat ataatgtctt ttcatgtagt
    11041 ttatcaaatt tctatttcaa accgcttaat acaaatatca aagcccaaaa ttttctactg
    11101 gaagctttta gagcctaaaa tatatttttt atttgatcca aaacttcagg gaaatacaaa
    11161 cctaatatca acacagcacc tataattaat agcggaatca ttttcttaat aaaagagatc
    11221 acagcaaaaa ctatcagtac tgcaactaat actaaagctg ttttgggatt agaatataaa
    11281 aactctttga ctgtagacgt tacatttaag tatagttcaa tcaatacgcg aaaatctcca
    11341 ttacttagtt tatccataac cgttctaaaa tcgatcatat catttcctca ctctttccaa
    11401 ttttattgct tcttttattg aagaagcaac ttgttcatta ctatgcttat ctatatataa
    11461 tggggaaaag taatttttat caaaaaagta atttgcaaaa gctacatcgt cttctgtctt
    11521 ccaattataa aaacagtaat tcccactaat cggtatttca aaaagccccc aattctctag
    11581 ctccatgttt tctttttcaa acaacactat gatttttttt acatcttcta tagtttccga
    11641 atcactgtta agtagctcac gtaaatatat attttttgta ttactgcaca ctatatcgaa
    11701 gtttttcata atttctattc tccgtttcta ctaattcatt cctatttcaa attcacgtgc
    11761 ttgtctcatt ttctcttgct ctatcattcg ttgcgctact tcataatcat ttttcgcacg
    11821 atacatattt aaatcatcat tgattactct atcaattttt ctaataagct ttttatcttt
    11881 aaatttttca gaaatcttct tgaagtggac ttcatttttt tcacgtgcta attgtcgttc
    11941 ttctaatctt ttttggaaat catcataact ttgtttagca tatttcgttt taaattctgg
    12001 tttttctcta tcaaacttaa cgtttaattt ttttccgtct aatagatttt taagttcatt
    12061 acttttcaaa tttttttctt cagattgctt taaaagtttg atttgtccta ctacagcatt
    12121 acccattcga gcattaaaat ctttttttct atttaaataa tattcttgtg gattgtagcg
    12181 tttaccgtag gttgtttgat atagctcatc aatttcttta gtcatacgat catattcttt
    12241 tctttttgga ttatcctttg tcatataatt tgtgacttca tcaatctttt cttttgtttc
    12301 ttcaggaagc tcattgtagc cacgtgttgt actaggtatt tcttttttta attcctttag
    12361 gtactttact aattctttgt catatcttga attattttct acatataatt gaatcaaaga
    12421 atcttttaca gactgtttaa gttctaaagg aacttctttt ctcaacttag aaattttttc
    12481 aaactcgttc gttctatcta atattttgct accgaattta aaaatcatat cgtctaacgt
    12541 agactgtttt ctcttacccc tagcttgtaa aacaccatca tattccatta tttcacgagt
    12601 atttttacgc tccatagctg ttacatgaat atggatatgt tcagtatttc tgtgaatcgt
    12661 tgcaaaccaa aaaggatcaa ctagttcttc cttttcttgc atttttccca tcatagactt
    12721 tgttgcttca tatagaacat tttcatttaa ttcatttgtt tctggattat aatatccttc
    12781 ccttactaaa aaatcattat cgaatgaaat tacatcttgg aacatcaccg atccattatt
    12841 ttttgcttct atgaccgatt cttttatttt tgaaatgtct tttttagtga tatatctttt
    12901 tgtattgcta aatgctccgt ttactatttc attatctaca ttttctaaga tagctttttt
    12961 acgtgtcata taatcaatat atttatccat ttcacgtaaa tctttaatca ctggttgatc
    13021 tgtttcctgt tctatttttt ccaaagcttt ttctattcta gctaattcat ttagttcatc
    13081 ttctgaataa tcatgatcta gtttatcttg cctaatagcc tcttgtctgg aaatataatc
    13141 tacaaatttt tgaagttcta ccatgtgatt attctttttg ctttttccag tttcaaattt
    13201 tgctttaaga ataatgctcg cactttttct gttaaagata tgttcagtca tatcatcacc
    13261 caccattttc aaaaattttt tctacaggcg gttttgtact taatgctaag ttgcattaga
    13321 attttttagt gttcaatccg ttgtaaatct taatgacggt gaactaaaca ttgttccctc
    13381 tcccaaacga ctactttctt gttctggttc agcacgctct tcttctaatt ctttactagc
    13441 taatccacta ttaattaata gtttatagac agactcttct atactattta attgttcact
    13501 ataatcttta tgtgtactgt cttctttttt attaaagttt tcaagttgct gtttttgcaa
    13561 ttctattatc gtttcaagtt cgctaatttt taattttgct tcttctaagt ttttagctaa
    13621 ctcttctttt tctggatcac ggaaattttg attggctttt gttaacattg gtaaatttga
    13681 aacaacataa cggaacatat tggattttgt ttgaaatact gacttgttta cttcaaagca
    13741 cttattgaat ttttctttta catcttctgt tgctgatact ttgaaataag ggtagttcat
    13801 ttatttaaat cactcctttc gctaattaaa aaattgaata agcgttcata aactgaagtt
    13861 aactcgctaa tactttctac catttgttca tttgacaata aaagtttttc aactactaaa
    13921 tcaaattctg actttttata gtcaatcaaa tcgatctcgc tatcttggcg aatgattgct
    13981 aaaaaatagt cattcctact aaactttttt ccggtacgtt cttgaatttt attaactttt
    14041 ttatcaatct cttttacata ttgtggatca attcctctga ttgaaacatc catacacaca
    14101 cctctttcaa gctgttttta tctatctggt atatattatt atatcaaagt ccaaaggaaa
    14161 attaaagatt taaagacaag accaaggaat taatacaaag agcataaggg aaaactgata
    14221 gcaccttcct aaaggaaggt ggctaagttg gctgtgccaa ctggttttct ttcaaaatag
    14281 cttcatattt tttgctatca caaaaaaatc cattttcgac ctattttcgg tcataatata
    14341 gtacctactt ttggtcatag tttcgtccgt agttttggtc atagtaacta aagcttttac
    14401 gcccaaaaaa agagtggcaa gttttacctt gccactctct tttctattca caaaattggt
    14461 ttaaattcgc tttttttagc acttcgttta tatcatagca atcataatat ttacacaaca
    14521 tcattcctaa ttttaacgct tgttcctctt tatatttttt aagctctgca ttttttttgc
    14581 gcttaatcgc ttttctcttt tctttatatt ccgcttcaat tctcattaat tcttcttcag
    14641 ctttctcttg aatagtgaca atagaattac acattctctc taaactccct taaaataatt
    14701 taaacacgat cactcaaatc ggcttgcaga cttttctagt tgctttattt tttcctcaaa
    14761 ttgtttgatt ttattttcac tttcctctat tgaagattta ttattttcaa tagtcgtctg
    14821 catagaactg atttcatttt ttgtatctgt ttgttcgtct agggtttgaa attctaagtt
    14881 ttgattagct ttatcaatat ctttttgcac tactttattt gctaaattca ttttttcaat
    14941 tgattttttc tcatctttta tagaattttc tatagcttta atctgatatt taaaattatc
    15001 tttttgcaaa tcagaagtac tttcaacttc caatttagca tttgatttaa tatctttgtc
    15061 tactgcatag aattttattt ccttttcttt taagtcattt gaaggttcta attcaggata
    15121 aatatatcta gggttaactg tcacagaaat tacaccaaaa tctttaggca gattttgata
    15181 ataaattacc gtgtattcat ctgacaattt tacaactttt gtcttaacat cttcattatt
    15241 tttgttcgtt ataaaattta atttatagtc acttttaata ttacttagtg atttagtacc
    15301 tgtatcatct tgataccgca atactaattt aaacagtccg gcttccctat ttatttccat
    15361 atcatcaaca tacatattca aagaagacgt tacattttgg gctgaactca tttttacagt
    15421 ttcaaaatca taacttttcc ccatcacata aggagaaaaa acgattatca ttcctattgc
    15481 taaaaatcca agtatagcac ctaaatataa ttttactcct ttcgtttttt tatatacttt
    15541 gtcaatttgt gccttatttt ttttatttgg ttccttctta ttatgaatgg aatatttctt
    15601 attcatatac ttcacctcac tattttaagt acttgatttc ataattatct accaacactt
    15661 ggccgtcttt attggttact tcaaaaagca tatagactgt attttctgta tctccactag
    15721 aagattctgt tttataattt acttcagcaa aataattaat agtattggcg ttaacataat
    15781 tcgtatataa ttttgaatct ttatatttac ttttgaatgt tggatcactt gaatatgctt
    15841 ctcctttttg agtagtagat ttactttcta aagtcttttt actcgttgta atcatgtcgt
    15901 ccggcacgat agaatcagct acagaaggag tagctatttt ctctaaatca gctttctttt
    15961 cttctacttt ttcaatatca tataccggat atagctcaac aaatttctta gcaacttcag
    16021 caatttgttt atttaattca ctgccatcat cattagttaa gttcgcttct acatttgcat
    16081 ttagatcact aatattttta cttaacttgc tattttgttc ttctagttgt gtaacttctt
    16141 gatccaattt tttatttttt gcttgaatgt tacttgtatg aagttgagat aacaatccac
    16201 ctaaaactaa aataataatt aaaacaccac aaatatattt ccatttatta tcattttttc
    16261 tcttaatcat ctattttctt cctcctattt tttgactcta cgaatctctg gactatgtga
    16321 ttgccaatat gaatcttgcc aattgtgata accaattccg cttgaattac tgtcatacat
    16381 agttgtttca ttgacataaa tacctacgtg agaaatgaaa tcaggcgctc catatgttcc
    16441 tctaaagaaa atcagatcac cagcttttgc ttctttaatt gatactgctt cagttgcttc
    16501 atactgcgtt actgtataac tatctattgt aattccggcg ctaaattgat aaagataagc
    16561 tgttaatccg ctacaatcaa aacctgtcgt tggattttta ccgccccaaa cataaggcca
    16621 accttgatat ttttccattt ctgttttcat ttttccccaa aattctgaag gattagccgg
    16681 attaccacta cccccaccga tatactgttt aactaactct gcatagtaaa aattaccacc
    16741 atttaaatac aaatatgttt ttccatattt aattgatact tcatttacat atgaataagt
    16801 ctgtcctgta gtatttccta aacttggagc aacaacattt ttagagtagg cttcagccgt
    16861 ttcgatagta tgtgtttttt tgttttttcc taagtagtgc atgtaagcta ttccgtaatt
    16921 gtaagcttga actattccat catagttact ttccatgcca taacttttag caatgttata
    16981 cacattactc aaatgtttta gcccttgatc gatactttct tcatattgaa gtgtgtttac
    17041 aggcaatccg gctgattcgc tactttgcat taaatccggc aatttacctc cggattcaca
    17101 ttgcataatt gctaaagcat aaggtactaa ttctttaatt ccgtattttt cagcatattt
    17161 ttctaccgtt ttttgccatt tttgaacttc aggtgacaac ggtttgttgc caccaatgac
    17221 ttctgcacct cctgtattag ttgcagtttg tgttccttgt aatgtagcta aagaacctat
    17281 aactaaaagg ccaaccaatg ttatcagtcc tataactacc cctattttta tttttaaagc
    17341 aagacctatt ttctttgata cttgcttcat cccttgacga gcgagtgttg aagcaacatt
    17401 attatttttg ttctgctcca ttaaattcac ctccgatttc tattattgac caccatcgaa
    17461 tcgttcaatt tgctctggat atggatcaaa attaatcata taggtgtctt cactggaagt
    17521 ttgaactaca gcttgtccta cttctaattc tggtaaaagc ataatttctg tatcagtaat
    17581 tgtatttcct aaaacttttt ttatgtcttc caacaatgca gaatctaaat taaagaagca
    17641 tttaaaagta catagattaa aaatgtttct aatcacatct gctacttctg atccagaatc
    17701 tttagggata atttcttcag gcgtttgagt tgcaaaataa acacctgcac gcatttttcg
    17761 catttctcgc tcaaaactta gtacatattc acaagcatac ttgttatttg aattgataaa
    17821 tactgatagg taagagtttg atattatctc cgtcttttcc cccagtccac accgtacgtg
    17881 cgactttcac cgcatacggc gttccctcgt ctataaaaga cattctgttt tcccgtccta
    17941 caaatattta ttaagatatt tcttcgttac caacttttaa tctcaactca ttgagttttt
    18001 ctttttgttt tggatttaat tttagatatt ttagaagttc ctcaatcgtt tcactttgtg
    18061 tagcgtgtat tacccgatga acctcctcag taacaatcac taggttttca tagctatcac
    18121 ttttgctttc atgataaggg ataatatgat gacaatgcat ttcagataag ataagctcct
    18181 cacctgttac tgcacatctt cccttttgag cgatgaattt tgaaattcga ttgtccgcaa
    18241 attcaatcgt tgctctctta ctaagcactg gattgctaca caaccacgat atcttccatt
    18301 cttctacggt ttgttgcttc ttatgaatgg cttccctacc ttcttcggtg tatttattaa
    18361 tatttctttt tttacacatc ggatgtctgt gttgaatata ccctactggt aatattggaa
    18421 accctcttat atatcgtatt tgtcctgacc gtaggtaagg cttgtatcct tcgtactttc
    18481 cgtattgtcc ttcttttgag attccttgtt ctcgtagtcg tatttcaaaa cttggtctga
    18541 gttctcttgc aatttttcgc attgctacac tacaatgagt cgcaattttg aagtaattat
    18601 gaatcccaat aaccatactg ttatagactc caatatagat aaaggtatct ttgctattag
    18661 gacttttttg aattcgtttg atttgttttt ttagtttcac tttaatcatg ctttgtgctt
    18721 ttgtacctat atgcgtatta acaacctttt tgttaccttt ggtcactaat ttcatcgtaa
    18781 agcctaaaaa ttcactttct tcttttgaaa gatttgtgat ttttgacttt tcttcagata
    18841 ttggtaactt tagacgctcc tgtaaccaca ttttagttgc atagaaaact tttttcgctt
    18901 cattataatt tctacagaaa attttaaaat catctgcgta tctcactagg tatatttctt
    18961 ttagagttgt actctttctg agcttgtgat agatattacc tttatcccaa gctccatttt
    19021 tcctaaaatg atgttttagc tctttggcat ctttttcctc ccactgattt gctatccacc
    19081 agtcaaattc gttgagattg atgttcgcaa gtaatgggct aagtattccg ccttgtggtg
    19141 ttccttttgt tgggtatatc aacttcccat ttggaagtac tacgggtgct cttaacattt
    19201 tcctaatgat taagagaagc tgtttgtctc gtatacccat agaccagatt tgtctcatga
    19261 gtttagtatg attgacttcg tcgaagaaac ctttgatatc tacatctacc acatattcca
    19321 tgtgactttg attcattcgg gccatacaat ctgctattgc gtgttctgct gatctatttg
    19381 ggcggaaacc atagctgtga ttattgaatt ttgcttcaca tattggttct aatacttgta
    19441 agatgcattg ctgcgcgatt ctatcccata ttgatggaat acccaacggt cttaatttac
    19501 cattatattt aggaatttct acacgcctta ctttccgagg gttataatgt gcgaatcgct
    19561 tcctaacaat ttctacgaat tcattacggt tgagattttc aatatcttta atcgttactc
    19621 catcttctcc tcttgtcaca ctaccgtgat ttctcttgat attacgatat gctaggacaa
    19681 tgttgttatc agaaatgata atctgcatta gattcttgaa attatctccc ttcaaacttt
    19741 tggagtatat cttatcgaag atttccgtca tgccataata ttctgcatgc cgcaacttcc
    19801 tatcttttgg acttgtttct tttgtcatgg tttatcacgc tccaattggc tcgtgacctt
    19861 tttagtcata cgagaatccc atggttgaac gggtttcatg acttttacag acgaatcgag
    19921 cctgttcctc cagttccatt acagaacctt catcagtcgt gactctactt tcaccatcac
    19981 taaagtaagt tatttgtcat tcacatattt cttactacca cttcatcgag tatgcactct
    20041 ccatgttgat ggcttcccct gttcccttat atctatccac atgccttaac ttagatgcta
    20101 gcttttgccc cctaacttct cctacgcctg taacgcagca tggtctttca taacaacgac
    20161 ttttactcaa ccacaagtta gcacaatgag tgattttttg ctcactgtct ctcccatttc
    20221 taggagctat gcctttcgga cagtacattc gctagttcgt cagtacgttt gtacatactc
    20281 accatattaa gtttttttgg acacccgact tatcggatac accctttacc tctaaagaac
    20341 tttcgtggta ttcactattt cttttacctt acggtatcct tcagccgact tcaccgagct
    20401 tcacacaacg ttggtctgca taaactactt gttgcatgtc ggagtatcag tggaagccct
    20461 ttgtggcgtt acccactcat ttgacttctc gttataagag ttattacaat agtttttaga
    20521 ctacttgcaa cccacagctt ttcactgcag aacaggtcgc accattatgg cactcatcaa
    20581 tcatcgccaa gaagcgtttg aagtcttgtt cttccatttg tttttcttta attaaacgtc
    20641 gttgtcttct tccttgaatc aatgcctgat tccaaatcaa cgtcatagcc atgtacattt
    20701 gacattgttg tacccctcga tctaagcctg taatagagtc aatgtcaaaa aagaccaact
    20761 gttgttggtc aaggtgtcga atcgtactgt gaccatcaaa aatcatgcca tacgtctcac
    20821 ataaggattc tacgatggat tccatttctt cttttcgatt ctttttctct tgggaagcac
    20881 ctggtaattc cagttgtcct aaaaagtcta aaaattcttt gaacgtcgga taagcttctg
    20941 gatctttccc tgtgacttgc gtctcagtat ttgaacgcga atttgaggaa gtaaagccgc
    21001 gtggtactgt tccatactct tcataaaaac gatttaagta ggtcttaaat tctgctttca
    21061 tcgttccttt tagctgagga ctcactaact gtacctgata gaccactttg tctaagtgtg
    21121 tttgatagga cgctgcttca ttgatcttca aatctttgac tgatccatcg tcatataaac
    21181 tggatgcttc atcatacgtc cgtgtggcaa atatctctaa aggattgatc atcccgtcag
    21241 atccgtctaa acgcaccata ataccgttat attcctttaa gaaaggaata aagtcttttc
    21301 ccttttcaaa gccccaaata aagtctcctg cagctaaatg ggcttccgcg atcattttaa
    21361 gaacggtcga tttaccagct cccatcattc ctagaagcaa ggtattagta gacaaccgta
    21421 cactattttt atacgtcgca ttccagacaa atgggccgcc tgtcatggtt tccccatatt
    21481 gtgtacctcc tggatctttt agcgtttggt ggtggaacgg gaaacctccg ccaatgttta
    21541 acgatggtag tgattgccct ttgatatttc cgaaccattc agcttgttca tcgaacatgg
    21601 taaacatcga ttgccaatct cgtttggttt tatgcagata agtcaccgca cgataatcca
    21661 tcccttttag atttttgatc agttcctgcg cccgttcatc tacttcttga atcgtgtttc
    21721 cgtaaacatg tactctcact ttgacttgtt tagaaatttc cccattagaa gtcaattttc
    21781 gggcatattc cgctagctcc aggtattctt ccgcactttt atttcggtca gtcgcttttc
    21841 gttcattatc tgatcgatca tttaattcat ctaatgcgcg attgatttga tcaatcactt
    21901 tttgcttttc atctgtgcta acatctagtg tggccactgt atgaggattg ttcatgagat
    21961 tggccaacca aaaaagagta ggattttttg tgtagtgata aactgtcaca caccgtgaat
    22021 aaccatttcc ataggtcaca tactcttcat cgaaatttaa tccaccttgt ggcatcactt
    22081 gttttaaaaa atctaaatca tatcctttgg attttaaaat ttctatttcc cgatttccta
    22141 gttgctttaa tggatcaagt actttttgat cattttgtgg tgaaaaagct gttttaatcg
    22201 cttctaacat gtacagtcac ctcttcttgt ttgattcttt acttggtgaa aagattgccc
    22261 ttttcccaac ttacccaggc ttaaagtgtg aggaaatttt ctagtggtct cttccttcgg
    22321 aacatctttt tctaaccata tcaacggata tttcattctc tcttctccat tcctactcat
    22381 cattcatgtt gttcattcga tacagaatct tgatcaattc ttttttgtag agtggtttta
    22441 accctaaaat gccactagat cgcattaact gacggtttct ggtttgtaaa tccttgattg
    22501 tccttccttc aatgatgaaa aagaagctta agtcaggacg ttcttgttct gctcgtttca
    22561 attcctggat ttcctctact aatttctttt catattttct taatcgctgt agatttcttc
    22621 ctaaggattg ttccatacga ttcttatcta ataactgccg cttttgtacg atttgttccg
    22681 ttaaatccgt ctctgtggtc aaactaacta acttaattgt tggcacatac gtacgcatga
    22741 cggtggtcaa ctgattcatt cgttctagtt tctcttgttc atttaatcct tccacgtctg
    22801 tagaaactac acgataataa cgttgaaaat gatcttcttt agtaactaag actccctctt
    22861 ctgtacagga tttataagga aataaatcca agatagatcg ttcttctggt tttccaaact
    22921 gaaaaatgga tttcttatca actgaatttt gtcctttgac tcgcatttat tcatcctcct
    22981 tatacgtacc atgataattt cggttaggat ctaacgcatg ataatggttc ttgtccgtaa
    23041 aaaaaataga ctcgataatc gcaaaaattt gtcgttgacc tgggttatta tagggtcgcc
    23101 aaacaatcac tagagctatc gcaatccata agccataatt caagagtgca aaaaagagtg
    23161 gtaaatggac aaggttattg actattttcg cccaaaaaaa gcaaccacca agcgttaata
    23221 gatccaaaaa gaaaaaggca aaaaacttcc attccccact aaatcttgga ggatttttca
    23281 tgttaatata tttatccata aatgatttct ccttttcata catagaaaaa aggcctaact
    23341 tgtaggcctt ttatggtttg atatcctgtt tgatatccct caatagttgt tctttcaatt
    23401 cattgttcct gtttttagtt tctcttgtca catttatttt tgttggtgca gtaaatgcca
    23461 catttgctgg tttgtaacct gtgtgaactc tttgaatagg ttctggtgca gcttctcttg
    23521 tcacaacatt ttgaacggac gcattccctg tctccccact ttcagataaa ttgtaagaaa
    23581 cagttgtcag atttccattt tcaggcaaac gacttggttt catttgaaca ttacctgcag
    23641 ttgattggtg aacaaagctt tgatcattct ccttgaattg attcaaacga ctatctttca
    23701 ttggtaaatt ttgtgcctta ggtaaaccaa ttttcgttga tttttcagtt ccaacttcga
    23761 ccccattatc atttggcaga tcgatggaat catcatccgt ttgtacttct tgatttcgtt
    23821 ttggtaaatc tgaaatgcgc ttttcatttt cagaattaat gttagattta taacgatatt
    23881 gttctggatc tgtttttggt aattgaccat tttgtccttc ttctgaattt tcgttagaat
    23941 gcaactcatc ttggtgatct ttggctgaag gaatcgctgc accttcttga ttttcttttt
    24001 cggttgttgt attagctttt actgctgcat tgacttgtcc ttcaccatac gcatctttta
    24061 gtccttcttt gacttctgaa gctttttctt tttgtttttc tagtgtttcg gatggattct
    24121 tcacagcttc agccaattct ttagctctcg tgccgtctaa tttctcttct aaggtgtctt
    24181 tggctttttc tgtaagatct tcagctgttc cttttaaccc acgttcattg acgtaaccaa
    24241 gaccttctgc ggctttagaa acaccatttt tcgctttccc gaacatttcg gaaactgttt
    24301 tcttactatt aggtacagtt gtagctttat cgtctgattg ttcttccccg ttatcagggg
    24361 taacatcttt tcgattacca aatccatttt tacgaatatc atttaaattg cttaaaccac
    24421 tcttaccagc acttgcaagg tttttagccc cgctacccac atttttcgca acatttccta
    24481 aggcaaacat gctaagaaga gaattgctgc cgtttttcag actcgtatca acgccgaaaa
    24541 ttttcgtaac tgattgagag cctttgaata gcgcaatggt taatacaatt aaagcgacaa
    24601 catacaagaa tccattgatc tttcctgccg catgcaaatc accgatacct gaaagcatta
    24661 atgtatagat tcgtaattct aaaatcgtaa atgccaataa caagaaggat tgagaaatat
    24721 cttctatagc acttttcatc cgttgtcctg tctcaaagtc cgttgacgcg gcaatcacta
    24781 aatagaattt tttaaatacc agctcaataa tgcaagtaac aatcgtaaac aagataaata
    24841 aataggctac cgacaaagaa gattcaccag ctaaaataat acctggacta ctaatgtatc
    24901 ggcgatagcc tgaagtatac acatattttg ctaaaccttc atcactaatt tgaacaggga
    24961 tttctttccc attatcgtct aactctagtc gatactgtaa tgcctttaac cgatcttttt
    25021 gtactgtact aatatcactt gattcagaca ttttcttagc ttgatcaggt gtaataatcc
    25081 cattgatatt cgctgtttta aaatttttcg ccgtgagagc atttcttttg tctgttggta
    25141 tgtgttctac tttgttcgga tcattttcta gtaacgtact cacatcaatc acattatttt
    25201 gaataatttg aaaagcaggt gaaccagttt tatctgcttt atttgctgca gtcacgtctc
    25261 cgtaaaagtt tttactaata tttagccctt catcgattaa agaactacca ccaaaaatca
    25321 aaatcatggc cataaataga ttaaccaaga tatttttgat ttttggtgga tgtttactca
    25381 taaaaaattt taagcctaaa taaattaatg tgacgacaca aaaaagagcc aataccttac
    25441 taatcaaact ttgaaacagt tcattaacac cagcggcgtt caataagtct tttaaatcaa
    25501 agctttgata aattgttttt tctagtaaag tattgagatt atataaccct ttaatcaaac
    25561 cccataaaaa agtattacaa atccaactga atacatcttg atactgcaga taattaccat
    25621 aatcttgtaa gactttcgtt acggttttgg gatctgcatc cccatcccca aataagcctg
    25681 accaccagtc ggcaatgccg gaaaatattc ctccaatgat cattctttca ccgccttttc
    25741 agcttctagc aaaagtcgtt tccttataac ctctgtaatt tcttcatttt gacgcaattt
    25801 ttcaaggtat tcttgcaaac gatctatttg cttctcttca taaaattgaa taagtaactt
    25861 ttgtgccgtt gagttgtcta aagattgaac gattttaata aaaatgtttt ctttagcttc
    25921 ctcttctatt tgatcgtgca tattgtgata aagtcgacgt gcatagatat caggtagaaa
    25981 ataatcgtaa tcaatttgta aagccttaag atccaagtcc gcatgtgtac aatgtatgtc
    26041 tatatcattg atatcccacg atggattgaa atcttcaata tattgataag cataaggaag
    26101 attcgtttcc ccatgattaa aaataggata cggccgtatt ttatgacctt tcaaatcttg
    26161 tcgttttaaa ggagcgagta caatcgtttc accttctaaa aaagtagaaa ttctttcagg
    26221 agttaagatg cgatctgaat caacattgcg attgatatga gtattcatac ctaatgaatt
    26281 gatggtactg ttctcttgtt caaccgtctt attccccact tttttggaaa attcttctat
    26341 ggtattatcg tctgtcgaca taatataaat atgtgtctga caattttctt tgataatcgc
    26401 tgcgttttgt tccccatact tcgcataaag ttgctgatag gattggataa acaaatccca
    26461 caacatgttt ctaccagctg tgactgtcat gattccttcc atattatcca ttggtggcat
    26521 attcccaaat tcatctaaaa taaaatgaat cctacggtga catttatctc ctttagtctt
    26581 aacacattgc gtagataatt ctttatacaa ctgactaaga aaaatactaa caatgatatg
    26641 gttagatgga tcataatcag gtgtgagcat aaaaatagcc attggtttat cggtatagtg
    26701 catccgaata gactctacta ataagttact tttatcctca tctaatgtta gaacggcttc
    26761 tttttctaat tctggcaaat gggtttctcc tcgttttttt cgataaacga tatttccctc
    26821 ttcgtctttc aatactcttt gagaaaactc ataccaagca acatattctt ttcctcgatg
    26881 atcgtatttt aagcaaacat gatctccctg ttctaaatga caatcaaagt taatttcaca
    26941 aaaacctcta tagcctactt tgacacgttc ctcatgaaat ttttttcctt gatgataaaa
    27001 ctctacaaca attcgctggt ttaacagact ctcgtgtagt ttgagatcca aatacttcgg
    27061 aaaaccaatg gacttataat caatggttga ttgagaagtc attttcgcaa ttttcgtcag
    27121 tgtaaatgga tttagtcctg tgatcgctgt cgatagaatg ttgcctttcg ctttatcaga
    27181 accaattttt gttgccccat attgtttttt tgctatgttt ccaggtggta actgattcat
    27241 aaattcatct aaagcattga gctgcttgaa ttcattatca ggatcttggt aattaaaacc
    27301 accaatctca gaaagaagtt cgacgagatt attcggtgtt aatttggagt aatcccctgt
    27361 ctgatccgct tgttccaata acgcaaaaat aagcgcattg acagcttttt gcgcattttc
    27421 gttaaagtat ttattgtctg aagcgttggc atcataataa atcgtattcg tgaaagtatt
    27481 ggttaactgt gtcgctgtgt ctatgtctcc tcgcttccag tatttcacga tgagtgccaa
    27541 tggattatca ctcattgatt gcagtggatc ggtgatatta aatgcataca catcataccc
    27601 tctagcttct agttcatctt tactagcaac atataattct gtttttggat cattaacaac
    27661 cattgaactt tgtctttctg cacgagacaa attgtcaatg gttgctaaaa cttctatctg
    27721 tcctttccct gaacgggaag taccaacaat acaagtattt actgtatccg tctgaatata
    27781 ccagtacttt ttataatgag taattggtat tccgccataa ccaggaaaac ctccctttga
    27841 ttctggtatt tgcggataaa tttgttggat ttcctttaac gtttttaatc ggttatctcc
    27901 tttttgccca tctgctagag gacgaaacca catgtccatt ttcaatccca taagtacagc
    27961 tggaacaaca caaatcgtag aaacaactaa acgtgtgcct aaacttgttg ttggggtcaa
    28021 aggattccaa aaaccagttg aatcaaaatg gaataactgc ggcatttctt gtaactgaaa
    28081 aatattcact ccttgtaaac ttcctggaat aatctcaaat cgatgaaatg cttctggtat
    28141 tcttgtaaca acatagttta aaggaagata agctaagcca ctataaaaaa tcttttgaga
    28201 aatacgtttg tcatgttttt ctggtagctc ttctcctgaa ggagtatagc gatagaaatg
    28261 agaaagatac ctattctttt ctaaattgaa tcgtttgtct tttcgtctcg ttgtttctct
    28321 aacacgcatt atttcacctc atttgattct gaactttcgg aagtgtttga ttgtacagtt
    28381 gaatcgcttg atccttcttt agccttacta ggatcactct ctttgctatt ttccatactt
    28441 gattctgttg ttgactgatt ggtacgattc aataattctt ctaattgttg ttcatactct
    28501 tttcttgctt gttcttgggc ttcttccttg attgacgtcg ttgtggccac agaatcagcc
    28561 tgtgaagatt gattgatatg attaaaagca aatactgttc ctccacctag aagcaagctt
    28621 aaacttgcaa tacatcccac catccatccg ttaaaggaac gctgtggtga aggagttaga
    28681 tttgtttggt tgttcattgg ggtttgaaca gcagctactg aaggcgttgg ttgaggttgt
    28741 gctagataat ttaactgagc ttgtcgcaat gtatccattt gttcttgacg ttccttacga
    28801 gcagctgctt ctttttcctt ttcttggttt aaccgttgtt tttctattgc catttgttgt
    28861 tccgcaagat cctgttcacg tttttttagt tctaattgtt cgcgctcttt ttctttttgc
    28921 cgttgttttt cttcttgttc tttcaattct tgttgttgaa tttcatactc ttttttccat
    28981 tttggaattt gcacttgtaa ttcttgctgg atcttttcgt atttttccat aagaacttca
    29041 gtcgttgttt gatatagctt attcagttgt tcatttaatt ctttgctaat ctttagctta
    29101 tctgtacgca aacctcggta aaattcttct tttcgttctt taatacgttc ttttaacaag
    29161 ttttcttttt ctttcgataa cgcttcttga ttcgtctgag taaaattatt gatctttgct
    29221 acacacaagg aatacgcatt gtcgcgtttc ttttcgtatc gctctttaat tttttgtact
    29281 tcttcttcaa tcactcgctg ttcttcttta tttagttcct cttttgcttt ctcccaatta
    29341 gacgactgtt ccgtcgcata ctcattaatt tcttgatctg tttcttgttg acgttcttca
    29401 aaggtggcta ataaatcttc ttgtgcaagc tcatgccaat ttgttgattg gacagactct
    29461 aactgttcta aaagtgtgcc tttaccctgt gagattaatt cttctttact ctctaaaaat
    29521 gcttgatagg tttggggtaa ttgatatttc gaataaagct catttaaaaa taatttgttt
    29581 ttttctttca tccattcatg ctctggcgga tattgatcca aaatataatc aaatacagtt
    29641 gccttttgta tctctatttc gtttttttct acttcttcac tgataagtgg ttcaatatat
    29701 tcctgagaat ccccttcagt ccatgttgac tcttcattcg cttcaactgg atcaacttct
    29761 acttcggccg tctcttcatc ctccattgtt tcttcaaagg gatctacctc caattgaccc
    29821 atcacatcgt tctcttcctc aaatatcgat ttctgagtcg gaatatcagc ttgttcgtca
    29881 acatagactg tctctttttc ttttgactcc ttctttggaa atattggtcg ttcccatggt
    29941 acttcttcat ttacttcgac ttcactccct tgattcactt ttctttggtc gctctctaac
    30001 tcttcttcta aaacttgtaa aagtttttcg tttcgataag actcttccac actcatagtt
    30061 tcatccgttt ctttaaacac atcaaggcgg attaaatcca tcaatgtagg atagaaacct
    30121 tctgaaattt gataatccag tgggccaaaa acgtattctg taccagcttt ctctattttt
    30181 ccttctgtgt catgtaacgt gagagtcaac ttaacagaag atcgactttc gtgttccagt
    30241 actttcccta acacatgtag ctgttcttcc gtttcaatga cagaatattt aattgttttc
    30301 ggtaaaaaag aagtacgctt tcctacttcc ttcgttgctt tttttattaa ctctttatct
    30361 gttttggtat tgcctgcaat cgtaaagtac atgagtattc tcctttcata aaaaaggagt
    30421 aaaaagtatc gactacttct tactcctact tttttacttt cttataatcc ttgaggtgtt
    30481 tgtccaaaca atccaaataa cgaaaccaca atagaagctg ctgctaaaat aactcctacc
    30541 cccatcaata catagcctac gtgcatcttc ccattttcag ctcctcttcg tccaaatagg
    30601 aagaaaagac ccccaataac aatggaagca acggccgcag cagcagcaaa aatcatcaga
    30661 ttgtttttta aactagttgc ggcagtttgt aatgctctaa aaatatcaac cgcagctact
    30721 actttcggtt gtgttaaaaa attcattgta attgattcaa acataaaatt gttcgctcct
    30781 tttagtgttt aataatttca tcgaaaacaa gcttttcacg ttcacgttga tagatatatc
    30841 cttctaagtc cttgtctttt ccccgtaata ggtcaaattg tgctttttca aggacttctt
    30901 gtttccactg tgtttcagat acaccgcggc gttctaagat tttagacgcg gctttttcaa
    30961 atggcgtttt agcttttgcc actgtgattc ctccttcagg acaaataaaa agactagcaa
    31021 tcactcgcta gtcttttgta acttatattt attggattgt gatccatcgt gaatcaattt
    31081 ctctttcgtt cgcttttcat ccttgctaga tccaagaaaa attatttttt ttacgccatt
    31141 cttctcatag ggaacaacaa taaataaaga atcagatttg tttataaatt ccctaacacc
    31201 agctgaatct actattttgt atctttctaa acctttatag tctttttgcc tcttgactgt
    31261 tatttgtaca ggatctggat gccatacact ctcagagtat tcccaggtag tctcgtctat
    31321 aattttcaaa ttataaacaa gaccacctac ttcttctttc cacatagttc ctgatttcaa
    31381 tacgccatta tcttgattag tttgtttatt tttttcatta ctacatccga ctaaaataac
    31441 aatcatggca atcaaggaag taactactat tattaacttt ttcattttat tcattctttc
    31501 gtttttttat tttaaatgta tctcagaacc gcataactaa aagttgctaa cacaagtaat
    31561 agtaagctga ccgctgaaat cgttactaaa tgtgtagaac ctgttttagg aacaatgacc
    31621 tgtggcaagt taggatcaac cccaattgtc acaacatttg ttttattaga aacattatct
    31681 actgctacta cggcttggtt atcaatattt ttttctgatt gtaatggagt tttagctgta
    31741 aatatcagct gaatttctcg tccatctaaa agattgttac ttaagaagtc agcggaagct
    31801 tcccacttca gcgttcttga attgctatca taggttagtg tgccttcaga agtaatatct
    31861 tttcctttac tcttatctat tacttgcgca gaaccttctt tatatgtcaa acgagcttca
    31921 attggatctt gaatactaaa ttgtttgtag tgatctgacg cagcgtctac gccttttgtg
    31981 tgccattttt gtgtgactag atattgtact tcagatcctg cgtttaaagg agctgtcggc
    32041 gtacccttct taccgtcatt tcgtccgttt acatcttttc cattttgttg tacttctttc
    32101 actggaattt tagggaaata aacggtaatg atatttgtat ccttttctac tttattaatg
    32161 atcgacgttc cctgattttt ggctacacta ttttgattgg ccaattcatt gacttttaca
    32221 ttgatcacta attcgtaagt ttcgccgtta tacttcattc gattttttaa tgtatcagct
    32281 ttcgctgcat attttacctg gtgttttgtt ttgtcatagg tcacttcacc agatacatcg
    32341 aattttttat ttgtaccact ttcaacgtgt gcatcgacgt aatttacttc ttttggtaaa
    32401 ttatcgatta actcaaatcg atcgtaaacc gctagtaaat ccactcctaa tcgattcacc
    32461 ttctgtttga ttcgatattg aatcgtgtct cctacttgca acattttatc gttcacgtta
    32521 tttccatttt tatcaacccc tgttttttca ggttgatcag gaacaacact aaagattgtc
    32581 gcagaagata aagtaaacca ggtttgaaca ttagaagaac caatcacaaa cgtcggattt
    32641 gcttcagata ttttaaagga cacagtacct tgaccaaact tcggatctcc taatttatca
    32701 tgcaaattat tgaaatcatt atccgcttgt gtcagcttag agcttcctcc tgggtggcca
    32761 cctaccacgt tcagattgtt gtagaaagat ttaaattctg aaagaaccgt atcttttctg
    32821 acataccaat ctgtcgtatt cattttgtca tagtgggcat attctccttt aagatccgtg
    32881 ctttttcctc ctgcagggtt caaagagtta aaactgataa atgtatctcc tgaaaattga
    32941 acggggctac cgtcctcata tgtggctact agctgcacat tcatttgtgt tgaatggtaa
    33001 acaattcctg agaaaagatt gtctgagatc caaagtactg ttcttttttt agatttactc
    33061 attgcaccat tcgtattttt atagccatcg tctcgaaaat gagtttttgt atagttgtta
    33121 tctagtagat tccacggcaa cgtatccgag tataagtgaa tattagatag aactagcttg
    33181 atattgactt ttttcccgtt atacgtaccg atttttccat agtcaatatt gactttggtg
    33241 ttggttgtta cgccttctaa aacaaggttt gtttcaccgt attctttttg gaaagaagga
    33301 tagcgtatat ttttgtttcc ctccactaat cctacgtagt cgtctgtaaa accacctttg
    33361 ttggcatcaa catgatattt gtaaactttt gacgctccgt ttacagtaat atttgttttt
    33421 gcattaggaa catcttttgg aataataaaa ttattcttta cgttctggat tggatttgac
    33481 ggatcgacaa taactttttc agcaaagaca ttggctgttt ttaacccaaa caatgtacct
    33541 aacatcatca ttatgggaaa aacatatttt aaccgtcttg tccacgagtg tttaaaattc
    33601 atcccttcat cccctctttt ttataaatga ctagtgaaat tccgacaata acgatcaaat
    33661 tgatcgtagt aaataatgga ttcctaatga gcttttccat aatatctagc ggcatggaag
    33721 cagctggtat gtttaaaatc ctcattaggt atagttggta gcttggcaaa atagatagtt
    33781 tagaaaataa taaaccgcag tacaaaaaga ctgcggttaa acaagaaaaa ttgaataata
    33841 ataatttgat cagtttcatt ttatttcttc cttaatctct agaaaacaag tgagtaacaa
    33901 aatcccaaca attctcaaag aagtttttct ttacgccagt agaaacgatt ttttttactt
    33961 cttctttagg ttgcgaagct tctgatttag gatagatcca cacatccttt aagaattcac
    34021 cgtttttatc agaaacaggt gttaatagca aaatatcctc gctattagaa atgttttcag
    34081 gacttgattc attgacaata taataagctt gtttctgatt tgcctgtact tctaccgaga
    34141 attcaaagac acctgtttgt tgatcaattg tctttgtttc ttcattagaa actagcttca
    34201 attgatcact ggatagtttt ttcactcgat tttttaattg ggattcaatt tggtcagtag
    34261 tgagatcctt aacatccatc ttttgtaaaa tgtccgatac atcataaaca gaaaacttcg
    34321 cgccatttac tccttcttgt ttttcatgcc cctggttgtc taattgttct gaagaaataa
    34381 tcttttgaac gtgaatggtc tgtttgacta tttgatcgtc ttcagcaatt ttaggttctt
    34441 ctgaagaatc aactgttgac gaatcgacgg tttcatcgct gctttgaatc gtgctgctat
    34501 ctgacgtttg gattgtttgt tctgtgctat ccgtgctttc ttgtctgtct gcaaacgtca
    34561 actgtgtgta ggcagtgaaa gaaaaaagag cggctgcaca aacaaatagc cattttctat
    34621 ttctcatgtt ggttaccctc ctttattttt tctttttata aacaaatagc gaaccaatga
    34681 tcatgactga aagaaaaaca gtacttccta aaagccattt ccaagataat ttttgtttct
    34741 tattttcttc atcttttaat tctgattttg taaaggttac tcgcttccca tacactagaa
    34801 aacggtgtgt gttgatcccc ttaggcgtac atgtcattag cgttactcga tcttcattgg
    34861 gatttgattc taagtaagtg acaaagtttc tttgtagttc gtctttttgc acttcctcaa
    34921 tccgatcgat ttgataagcg tgctgctcac catcaacttt gatataaaac ttttctccct
    34981 tttttactga aggtaaatca tcaaaaagat tatctttata aagtccacta tgacctgcga
    35041 ttaaaggatt tttaccgttt cctcctgtaa tatcaccagt tccttccgta atacctacac
    35101 cattttctaa aattttgtcg gacgttccgt cataaattgg tagttttaaa gaaatactta
    35161 gaattgtaag aataccaaca ggatcgccta actcaatctt agcaacatcc aaagaagaat
    35221 ccgtttctgc tgccgttcct gttcgatcga ttgcggcaat ctgctgattt tgttggtatt
    35281 tcgccacgct cgcttcagca ggcatcaaaa gtaagacacc tacagctgaa agtaaccaga
    35341 aagcaatgat ccatttaccc atgattcgct ttttcttagg cttcctgata atcattggtt
    35401 ttgatccttc taggctttct tcttaaagta aacaactgca gaaagtgccc ctgctcctag
    35461 caacaagcaa gagattaata catacgtttg aaaaccgttg ctaccggtca ttggtacgga
    35521 agctttttga ttgtttttga tcgtgattcg gttttcttct ttgtatgaat ctttgtctac
    35581 tttgaaagag atctctttcg ttaatttttg atagttttca ggcgcttttg tttcttccag
    35641 tgaatattca ccttcagtta aaccagtgat ttcaaagtga ccttctttat ctgattttag
    35701 gatcgctgca tttgctacat cgtctaccca tactgcttct ttgacaccgt ctttatccgt
    35761 atcaactaat ccaccataat attttccttc tgcgttttta gcagcaaatt ctgcgccttc
    35821 aagcggtgtt gctttgattc ctgttccaaa tagtccgcta tcgactttta agaattttgc
    35881 accataagtc acaacgtcag ctttcgtttt ttcgtggtgt tcttcctgat taaatggatt
    35941 gttgttataa acgaaatcca cgctatttgt taatggattg gcaacagctg cgctgtcatt
    36001 tacatacatg ttataagtca tttgaagatc ttgtccgtgt aaacttgcta cagtatcact
    36061 tacttggcca ttattcacga taaaatcgat cacaaagcca ttttctgaat tttggatttt
    36121 ataatctgta ttttctttta ataccacaga tccatcttta tttgttactt tgatatttgc
    36181 tttagcagcg acataagaaa gtccttgtcc tgctgtatcc ccccatttaa atacactgta
    36241 aggagaagtt tcgcttttca ctccgtttac tgtgataccg tctgctccgc ctgcaatgtc
    36301 tgttgggata tgaatcgttc cttgataatt tactgcgtca ccaatttgga aagaatgttc
    36361 ttgtcctgtt ccatttgtta ctgttttatc gatcactggt gctttatagt ttacgtatga
    36421 agcttttaag tcacttgtca cgccatcatt cgtaatttca aaggttaatt tattgtgcgc
    36481 atcgttgcga gcgtctgcgc ctaataaata ttgatcggct gttggttctt tatctgatcc
    36541 cactacaact tctgaaggaa cttccacaaa atagtatttt cctaacgtca aatcagtagc
    36601 agtcagcttt ccttgatcat ccgatacaag atctcctaat tttgttcctt ttccaggttc
    36661 gcccttgtat aactcaaatt ttgccccttt taaaggtgtt tgtttgcttt gagctgtgcc
    36721 atcgtcacca aatttcgtta attcaaaact caacttagaa acaatatttt taggatataa
    36781 gtgaatgtct tttaaaaaac ctgaacctgt attatcagtc attggcgcaa tcacaaccat
    36841 tggttttgct ttttgtgtga ctaatccagc agcggattta ctttcataaa ccacatatac
    36901 attccctttg gaattgacat aggctggttg atcagcaaaa gtgatctcac cattttcatc
    36961 tacagctgaa gtaattttgt tggttgcatt cttgacgtac tcactgttag atcccttgtc
    37021 ttctacatct ttaacgattt cgtccacctt cgcgttggtt agatcagaat cttcagtcgg
    37081 taatacttga tccgtaatat ttcctaacgt aaactcaaca tcaccataat ctgccttgtt
    37141 gtatttcgtt acacctgtag gatattgatc gtgcgtataa ccatcgtttt tgatcccgtc
    37201 aatatcaaca ttcaattgcg ttccttgatc gtacattaat ttatgaaccg tgatattgac
    37261 cttctctggt ggcgtttttt gcgtgacatc ttctgcatat gctcgatatc cctgaaagca
    37321 gcctgcaatg attcctaaca ttccaataag cattccagct ttttttacat accgatttat
    37381 tgccattttt tactctcctc atttcatatt tcaacgactc acgttgatta aaaccttgtt
    37441 attgtagttc tccctgtacc acgtatcttt cttttccttg tgacgtataa acacaagtaa
    37501 acaacgttaa taagggtttc tctcctttct ttggcaaaga caagaaagag ttctcttcag
    37561 gaacgccatc gattaattta aagtactcag gtacgataaa tttacgcgtt accttgtaac
    37621 gatagacgcc atcgttacta actaagtcca tcacctcccc taatgacaac ttttgaatat
    37681 cagaaaataa aacacctggc atttccatgt tatgacctgc taggacatag tttcctttgc
    37741 ccatttcagc atcttcataa aaataagtag ccgctccaag actcaaagtg tattgatttg
    37801 cccctttaaa aattggcaat gctaaaccaa gtgcaggaat ctgaatttgt gcaaatactt
    37861 gtttattgac gcccatttcg attgcttctt gttgtatgga caacatttgc cccaaagaca
    37921 tatcattatt gacgttcgtt gcatcctcct tataactctt tgcatgtgca ttgatttgat
    37981 ctacgggcat ctttttttct atattttgct taaacacctc tccttcatgt ttttggtagc
    38041 taacatagcc aaacacactg caaaagacga ttaaagcgac tcctaaaaaa ccaaataacc
    38101 attttctcat ccagctaccc cctttcagac aaaacaaaaa atctttttct catttccata
    38161 gaaaaagttt taaaaatgaa ttctattaat gaatgtcatg agacttctaa caatagttga
    38221 caattagaac ttgttttcac ataagttcgt ctcaactgtt caaagttttt cattgaacct
    38281 aacaaacaag ctttctcttt tcctgttatc tgcatatcat tgatatattg catgacagat
    38341 aatcttttaa ggtgtttttg tttcatcgtt tttagcaact tttcttccat ttatttcgac
    38401 actttttttc tgttttcgat ttcttcataa ttcatgagtt ttcctacctt ccttgttttt
    38461 tgcaaaaata aaaaagccta tgatcacata cgctctacac ttcagtcttg ggttatcttt
    38521 ttctttcgtt taattactgg ccgtgctggt ttcaaatcct tgattccatt atgggttctc
    38581 caataatcga attcttttcg taatctttct ttttccatat ctgttccttt agacagggat
    38641 ttgaatacat ctttaggtga ataaccacga gctaacagct gtacatacct tccttttgtc
    38701 aaagtatcca tatccatcgc ctctaaaact ggtcgcgcca tctaattttc ctcctaaatg
    38761 aattccttac tcaatgccta aggtaatact acctttttat tattcgtcgt gacttcttct
    38821 aagcctttat ctgtaaaatt tattcctcca gtttttattc ttctttatcc gctacaaatt
    38881 caatgatttc aacgctgaca atataatcac tcaaatatga ccaagtatct tcaacagata
    38941 attcgtctcc attatcattc gtaagcgtca ttgtgaacta ctcaccactt agctaaacct
    39001 aacggtttct tacgcttgaa gtgggagctt cttgggaaga gagcatactt gatagcgtat
    39061 tactttacta acagaggtgc ctcttattta ccaagctatc cccgtagttc ctacggttct
    39121 tgtttatttg atc
//
LOCUS       DACEOM010000020        38665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEOM010000020 DACEOM010000000
VERSION     DACEOM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.021"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            23..646
                     /locus_tag="IW781_000993"
     CDS             23..646
                     /locus_tag="IW781_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182006.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IW781_000994"
     CDS             1096..2145
                     /locus_tag="IW781_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1182007.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IW781_000995"
     CDS             2142..3023
                     /locus_tag="IW781_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182008.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IW781_000996"
     CDS             3026..3796
                     /locus_tag="IW781_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1182009.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IW781_000997"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IW781_000997"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1182010.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IW781_000998"
     CDS             5107..6270
                     /locus_tag="IW781_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1182011.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IW781_000999"
     CDS             6387..6797
                     /locus_tag="IW781_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1182012.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IW781_001000"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IW781_001000"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1182013.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IW781_001001"
     CDS             9337..9654
                     /locus_tag="IW781_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182014.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IW781_001002"
     CDS             9814..10050
                     /locus_tag="IW781_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1182015.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IW781_001003"
     CDS             10047..10445
                     /locus_tag="IW781_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1182016.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IW781_001004"
     CDS             complement(11112..12056)
                     /locus_tag="IW781_001004"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1182017.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IW781_001005"
     CDS             complement(12352..13296)
                     /locus_tag="IW781_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1182018.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="IW781_001006"
     CDS             13517..14731
                     /locus_tag="IW781_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1182019.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="IW781_001007"
     CDS             14753..16768
                     /locus_tag="IW781_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1182020.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="IW781_001008"
     CDS             complement(16851..18224)
                     /locus_tag="IW781_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1182021.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="IW781_001009"
     CDS             complement(18250..18534)
                     /locus_tag="IW781_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1182022.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="IW781_001010"
     CDS             complement(18536..20551)
                     /locus_tag="IW781_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1182023.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="IW781_001011"
     CDS             complement(20730..21035)
                     /locus_tag="IW781_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1182024.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="IW781_001012"
     CDS             complement(21124..22155)
                     /locus_tag="IW781_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1182025.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="IW781_001013"
     CDS             22362..23237
                     /locus_tag="IW781_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1182026.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="IW781_001014"
     CDS             complement(23389..24231)
                     /locus_tag="IW781_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1182027.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="IW781_001015"
     CDS             complement(24323..26200)
                     /locus_tag="IW781_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1182028.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="IW781_001016"
     CDS             complement(26197..27618)
                     /locus_tag="IW781_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1182029.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="IW781_001017"
     CDS             complement(27621..31010)
                     /locus_tag="IW781_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1182030.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="IW781_001018"
     CDS             31267..32658
                     /locus_tag="IW781_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1182031.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="IW781_001019"
     CDS             complement(32731..33378)
                     /locus_tag="IW781_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1182032.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="IW781_001020"
     CDS             complement(33386..34072)
                     /locus_tag="IW781_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182033.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="IW781_001021"
     CDS             complement(34078..35151)
                     /locus_tag="IW781_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1182034.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="IW781_001022"
     CDS             35373..36047
                     /locus_tag="IW781_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1182035.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="IW781_001023"
     CDS             36052..37392
                     /locus_tag="IW781_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1182036.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="IW781_001024"
     CDS             complement(37541..38527)
                     /locus_tag="IW781_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1182037.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11903 a         7049 c         7319 g        12394 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga atgaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac attta
//
LOCUS       DACEOM010000021        38664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEOM010000021 DACEOM010000000
VERSION     DACEOM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.022"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(369..995)
                     /locus_tag="IW781_001025"
     CDS             complement(369..995)
                     /locus_tag="IW781_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182038.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="IW781_001026"
     CDS             complement(1290..1625)
                     /locus_tag="IW781_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1182039.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="IW781_001027"
     CDS             complement(1612..1896)
                     /locus_tag="IW781_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1182040.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="IW781_001028"
     CDS             complement(1952..3475)
                     /locus_tag="IW781_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1182041.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="IW781_001029"
     CDS             complement(3468..4643)
                     /locus_tag="IW781_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1182042.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="IW781_001030"
     CDS             complement(4647..4832)
                     /locus_tag="IW781_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182043.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="IW781_001031"
     CDS             complement(4798..6492)
                     /locus_tag="IW781_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1182044.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="IW781_001032"
     CDS             complement(6489..6962)
                     /locus_tag="IW781_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ1182045.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="IW781_001033"
     CDS             complement(7031..7186)
                     /locus_tag="IW781_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ1182046.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="IW781_001034"
     CDS             complement(7320..7700)
                     /locus_tag="IW781_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1182047.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="IW781_001035"
     CDS             complement(7704..7919)
                     /locus_tag="IW781_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182048.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="IW781_001036"
     CDS             complement(7922..8329)
                     /locus_tag="IW781_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ1182049.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="IW781_001037"
     CDS             complement(8595..10070)
                     /locus_tag="IW781_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ1182050.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="IW781_001038"
     CDS             complement(10060..10908)
                     /locus_tag="IW781_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ1182051.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="IW781_001039"
     CDS             complement(10945..11307)
                     /locus_tag="IW781_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182052.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11607..12231)
                     /locus_tag="IW781_001040"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="IW781_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="IW781_001041"
     CDS             complement(12228..12539)
                     /locus_tag="IW781_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182053.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="IW781_001042"
     CDS             complement(12583..12876)
                     /locus_tag="IW781_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182054.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="IW781_001043"
     CDS             13070..13717
                     /locus_tag="IW781_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1182055.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="IW781_001044"
     CDS             13778..14923
                     /locus_tag="IW781_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1182056.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15004..15309)
                     /locus_tag="IW781_001045"
     CDS             complement(15004..15309)
                     /locus_tag="IW781_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1182057.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="IW781_001046"
     CDS             complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="IW781_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1182058.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15762..16028)
                     /locus_tag="IW781_001047"
     CDS             complement(15762..16028)
                     /locus_tag="IW781_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1182059.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16283..17008)
                     /locus_tag="IW781_001048"
     CDS             complement(16283..17008)
                     /locus_tag="IW781_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1182060.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17369..17439)
                     /locus_tag="IW781_001049"
     tRNA            complement(17369..17439)
                     /locus_tag="IW781_001049"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17405..17407),aa:Gly,seq:ccc)
     gene            17556..18308
                     /locus_tag="IW781_001050"
     CDS             17556..18308
                     /locus_tag="IW781_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1182061.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18324..18803
                     /locus_tag="IW781_001051"
     CDS             18324..18803
                     /locus_tag="IW781_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1182062.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19054..20634)
                     /locus_tag="IW781_001052"
     CDS             complement(19054..20634)
                     /locus_tag="IW781_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1182063.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20627..21370)
                     /locus_tag="IW781_001053"
     CDS             complement(20627..21370)
                     /locus_tag="IW781_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182064.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21546..22322)
                     /locus_tag="IW781_001054"
     CDS             complement(21546..22322)
                     /locus_tag="IW781_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1182065.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22570..23406)
                     /locus_tag="IW781_001055"
     CDS             complement(22570..23406)
                     /locus_tag="IW781_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1182066.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23417..24346)
                     /locus_tag="IW781_001056"
     CDS             complement(23417..24346)
                     /locus_tag="IW781_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1182067.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24538..24891
                     /locus_tag="IW781_001057"
     CDS             24538..24891
                     /locus_tag="IW781_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1182068.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24965..25621)
                     /locus_tag="IW781_001058"
     CDS             complement(24965..25621)
                     /locus_tag="IW781_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1182069.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25847..27781
                     /locus_tag="IW781_001059"
     CDS             25847..27781
                     /locus_tag="IW781_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1182070.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27867..29090)
                     /locus_tag="IW781_001060"
                     /pseudo
     CDS             complement(27867..29090)
                     /locus_tag="IW781_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            complement(29084..30982)
                     /locus_tag="IW781_001061"
     CDS             complement(29084..30982)
                     /locus_tag="IW781_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1182071.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30976..32094)
                     /locus_tag="IW781_001062"
     CDS             complement(30976..32094)
                     /locus_tag="IW781_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1182072.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32177..33010)
                     /locus_tag="IW781_001063"
     CDS             complement(32177..33010)
                     /locus_tag="IW781_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1182073.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(33003..33920)
                     /locus_tag="IW781_001064"
     CDS             complement(33003..33920)
                     /locus_tag="IW781_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1182074.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(34147..35271)
                     /locus_tag="IW781_001065"
     CDS             complement(34147..35271)
                     /locus_tag="IW781_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1182075.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35454..36362
                     /locus_tag="IW781_001066"
     CDS             35454..36362
                     /locus_tag="IW781_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1182076.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            36503..38113
                     /locus_tag="IW781_001067"
     CDS             36503..38113
                     /locus_tag="IW781_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1182077.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAQIIIYERGENISYASCALP
                     YYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIKT
                     DYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAGV
                     MGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEIR
                     DKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPDI
                     YAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFDH
                     TASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQA
                     FGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTNI
                     VETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKVY
                     ITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        11701 a         7706 c         6343 g        12914 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    15001 agtctattct tcttcttccg ccatgaatgt attgaatact tcttcaatca tgtcccattc
    15061 tgcgtctgtt tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc
    15121 ataagcttgt aattctacat cttcatcttc tgaaacacca gcaggatata aaagaacata
    15181 gttacgtcca aattcttctt gtccgtcaac tgttagtagg atttcatata atgtttcatt
    15241 tccttggtca tccaccaatg tgatgtgttc gtgtccttca tggtcgtggg aatggttatg
    15301 ttctgtcata agattacctc ttttctttta ttattaaaat aattgcttac ctgagccgtc
    15361 taaataattt tgtaagatca tcacagctgc taatttatcg atgacttttt ttcgtttggc
    15421 tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttaatcgttc
    15481 atcttgataa tcaactggca aggagaatag ttctttgatc ttttctccgt atgacatgga
    15541 agcttctgcc cttggtccga tggaattgtt catattttta ggtaatccaa ctacaaaccg
    15601 gtctacttca tattgtgcta ctaattcctt caaccgatca aagccaaact cttcttctgc
    15661 ttcattgatc cgaatgatct ctactccttg agctgtccag ccaaaggggt cactgatagc
    15721 aacacctact gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga
    15781 tttgagaggt agtccttgac aattacttcc atgatctcat cgcgttcgtg acggcgaatc
    15841 aaatttcgag catcacgata acgaggaatg taggccgggt ctccagaaag caagtagccg
    15901 acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat
    15961 gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta
    16021 aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaacctt
    16081 attttactcg aagaaaggga aaactacaaa caaatattcc aaacttccga ataaattaag
    16141 aaacactatt tgtatgtttt tttccttttt tctgtagctt tatcaaaagt actcttttca
    16201 taaagtatac gctatatttc tttgaatgta tgtttttaca caattttttc ataatattca
    16261 taaaaaattc gaatagctgc tactaatttt ttgtttttgt tgcttgtcct gtctcataat
    16321 tgatcgtgta gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt
    16381 caatgctttc tgcttccaat tgcactcccc tggctaccag ttcattcccc tcaaaatgag
    16441 gagtgacacg gtagcgtaca tgatgatttg tttctttgat atatgccgca atatcatttt
    16501 cgtgagcaag catgtatgga gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt
    16561 tgttattttg accagtcagc tggaaaccaa ttaaatggct gcgattatat aaccattgcc
    16621 cgtcacttaa tttcttttgt ttccaacctg tcgggtcaat atacaaaggc tctctctttt
    16681 cagtagggaa caaatctttt ccaagcatag cattggcttg tcctactctg ttcaatttat
    16741 caatatttga atagctttcc catgttcctt tggaaaggct aagatcctct tttgtaaatt
    16801 ccggctgatt cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg
    16861 ctacttgaga agcttgtggt gtggaatcaa aaatagttgt aatctgctga aagatatctg
    16921 aatctttttg tgtggtggaa acaccgaatg cccctgcaat cacagcagcg acgatcccca
    16981 cgatcgcctt ggttgttttt ttgctcattt ctttactcct ttactaacag ctccctaaat
    17041 cttaacatat gttcccctat aaataaatac tagaaagaaa tagaaacagc aaaaaataaa
    17101 acctgtttta agatggctta aaaatacaaa aaagagacgg attcctcgtc tctaatcagt
    17161 caccactcta tctgtaaggt atttattatt attcaaaaat tttcttcata ctcaagacaa
    17221 tacactgatt tcacaaccgc ttggtaaacg gtgcgcaaaa gcttgcatct gcggtaataa
    17281 gtccatccaa gttaaaaaca taagaagttg tttttgcttg gctgttctta ttacttgttg
    17341 cagagcagct ttttcacaac ctattttaag cgggtgacgg gaatcgaacc cgcgacatca
    17401 gcttgggaag ctgggatttt accactaaac tacacccgca atacaaaaac agtatacgcc
    17461 tcttttattc cttcctcaag tttttttatt ctttacgctt ctggtatgtt ttgtagtaaa
    17521 ataaaactaa tcaatcaatt attggagaag atgctatgac gggaaaagaa ttagatctgt
    17581 tgctggacac ttgtttactc gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc
    17641 gggtagaaga tacgatgagt cgaattgctt tagcttctgg aaatttccgt ttggtcagct
    17701 atgtcactca aaccggtctc tttattggtc ttgatcgtac ctcgaccatc cgaatggaac
    17761 aaattaccaa ccgttcgatt aatttagaaa aagtagtaaa agtaaatgat ctttcacgta
    17821 aatatgttgc ttcagagcta acattagaag aactttactg cgaactgcaa gcaatcgacc
    17881 gagaccgttc tttctttcct gtctggttgc aaatcgttag cgcggcagtg atcagtggaa
    17941 caattatggt gttatttggc ggaaagatga ccgatttacc tgtgacttta gtcattggag
    18001 gattgggata cgcagcttat ctatatagtt tgaaattttt tagaatcaaa tttttatcgg
    18061 aatttttatc ttctttattg attggcatag ccgctatcta cagcgtacgt tttggcttag
    18121 gaagcaatca ggatctaatc attatcggat gtgtgatgcc tttagttcct ggagttcaaa
    18181 ttacgaacgc tatccgtgat ttgctagctg gacattatgt ctcaggagtt tctcgcggaa
    18241 cagaagcgat gatgactgct acgatgatcg gttttgcgat tgcttttatt ttccagcttt
    18301 tttattagga aaggaacaca agtatgggga cgatatttat tcagttttct tttagttttt
    18361 tagcgactgc ggcatttgcc gttattacaa atgtacctag gcgttcattg atttgttgtg
    18421 gtctttctgg tgcttttggc tggatgatct actggatctg tgttcaaata ggcggatctg
    18481 ctgcttttgg ttcattgctt ggttctttag gagtagcatt catcagcgat cttttctcta
    18541 aacgtttgaa aatgcccgta acgattttca atattcccgg gatggttcct ctagtccctg
    18601 gtggtttagc ttatcaagcg gttcgaaatc tcgttactga atcctatcga gaagcggctc
    18661 attatgcggt ccaagcaatt atgattgctg gagcaatcgc tttaggtctt gtattatcag
    18721 aagtctttaa ccataacatc cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac
    18781 gaaaaaaacg gctttttaaa taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt
    18841 tcagccttga gcgatgacaa aatatctcac aaacattgcg acaagtaacc gcaggagcaa
    18901 taagaagtaa ttatgagtat taaaaaaagt tttgtaaaaa tagcttctca catttttaca
    18961 taaaattgac ttaatactga gaaactgact tttgaacacc ctctatctgt aaaaaagggg
    19021 ctgcgacatg atttttgtca cggcccctct tctttattca aattttttcc aaaacgttcg
    19081 ttgataataa acgatccata gagcggaaag tattagaact aaaccgatca caggaatatt
    19141 cactaaccaa aatggataaa acaaagccaa cattccgtat aagacgataa gaataatact
    19201 gatgatcaat gaacccatca tagttgcgac taaacccaaa cttcctgaac ctctgttaaa
    19261 taattggtta atattggtcc agttcagcag cagcaaccga taatctctag cgaaataacg
    19321 tagtgacaat aagtagcagc cgaataaaga accaaatagc aagctaggga taaaaaacaa
    19381 aggcagctga aaagacagtc cgcttaataa tgcgatccca ccagttaaaa aactctgcaa
    19441 tatccatccg aatctgaact tttcttttaa atattggttc atagagattg gcaaagaacg
    19501 gataaacaga aaattttctt gatccaaaga aataagattg ctgataaaag aagtctgatt
    19561 aactgttaag aaagccaatg cgatccctac caggaaaaca acaccgatta acttattatc
    19621 taaatcagtt aattttattt cgcctcctag ggcaaaagca agaataaaaa tgattggagt
    19681 taacaaggaa gaagaaagaa cttgcataat caaattaggt tcttttacta attgagcatt
    19741 gtaactaaat aaaagctgat gcacattttg gttcttttta tgggttcttt ttattttgaa
    19801 ctcactgttt gaatcagtca gttgctctgt aagctttggt actatcagct gttttacagc
    19861 aaggaaaaat cccagagccg tgatagctag tattccccaa cccaataaac cttgttgaga
    19921 aagtggctga ctggcagcat catagaatgg caataatatc gaaatcggat gccgatctgt
    19981 taactcattt tcaatagtcc ccgtttgatg attcatccac atgattccag caaatgcgat
    20041 cagcatcgag ctgccaagca agatactaga aactgttttt ctatgttttt taaacactgc
    20101 tgttcgagcc aaaccaaaaa caatcaaact agccagacaa aaaagaataa tcaaaaatag
    20161 agaaaacaaa aggattgaga taatgatagt aagcaaaatc cctagatcag ctcgccatcc
    20221 ggttaaaata aacatgacta gaagcggaaa aacaaaagga atgatggtca aagcaactac
    20281 caaaatcttt gctgaaaaaa cctgttcttg tccaaaaggc agaggcaaaa atacttgcag
    20341 gtcatttcct tcaaaaaaga tattataaat gactgatata ctttgagtta aacctaacat
    20401 accaaaaagt gccacgtaat aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa
    20461 catagcaaat ccgtaaatca gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag
    20521 atagatagtt aatcgctttc ccttttttcc tttctctctt gctttattcg tgagctgtgg
    20581 attaacatat aaaagattaa ctcgaattaa ttctaataat tgtcgcttat acatacggtc
    20641 catctcctgt cgcttgcctt ccagccattt tcaaataaat cttttctagc gtttcatcag
    20701 gtgagccatt caataattca ctaactgccc cctgataaat caattcacct ttcttcaaaa
    20761 tagccagttt atcgcataac tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg
    20821 tttttccttt tgcggcatga gccttcatca tttctttcaa atcaaatgct gcttgaggat
    20881 caagtccttg caaaggttca tccaataccc aaatatctgg atctggcagc aacgtgccga
    20941 tcaaaatcgt tttttgacgc attccatgtg aaaaacttgc gattgtttca ttttgatgac
    21001 tatacatatc aaaaagcgct gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta
    21061 attcataggc agctgaaatc aagtcccaat attctccagc cgtcaattgt aaaaaaatat
    21121 ctggggaatc aggcacataa ccgatttttt gtttgatact caatcgattt tccgtcaatt
    21181 tcattccatc tactaaaatc gttcccgatg atggttcaat aatactaact aaacttttaa
    21241 tcgtagttga ttttccggca ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta
    21301 tcgagagaga caagtctttt aatgcttgtt ttttcccgta acttttactt acattgatga
    21361 attcaatcat ttctgtactc cttttgtttg ttattacttt ttattgtacc cttcgttatt
    21421 ggtggagtaa atgtcaaaaa gagtgcataa caaaaaagga tagaaacgac taggctgcta
    21481 cttgggttgt tctcttctac aggaaaacac tcttctaaca ggtaagatat gagtctttgt
    21541 tttctttaaa atgattcatt ttctatcttt tcttgcataa aattctttag tttatcaata
    21601 cttctctttc ccatctctag ttgttctcca tttgcaaatg ttactatttc gtttactctg
    21661 ctaatagatg taatgttttc agtattaatg atataagctt ttaaatcttt acataaatac
    21721 tcatattcga gtgatttttt atatttttta attgtgccgt ttacgagaat ctcttcatct
    21781 ttcaatacta ggcctatttt atttttataa cctggcatgc tcattagata aaggatttca
    21841 tcgtaatgga tatagtgatc gctattacca taacggacag ttgtccacct ttttttcgta
    21901 gcatgttgaa caggatagtt ttctttttct ttcaataagt aactttggat gcttttttta
    21961 tttttttcat aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata
    22021 acagttattg ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt
    22081 tctctaagca tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg
    22141 aaaaaaatat ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg
    22201 ttcaatccct gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa
    22261 atttctacta gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc
    22321 ataaaaagta cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca
    22381 tacatgttag gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga
    22441 tatcaaatac tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg
    22501 caaaaaaagc cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat
    22561 tttgttcttt taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc
    22621 gtttgtaaca actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat
    22681 ggttccttct attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt
    22741 caacaaacca aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa
    22801 gatatctgta ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt
    22861 gccaatgaat tcgctagggt aattataaat tagctccata acttctaaat agttttcatc
    22921 tgtgatcttg atagtttgct tatccccata cttttctttc aactcattca tttggtctaa
    22981 gtagtctgat ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc
    23041 cggatcccct actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt
    23101 agtgtcgaga ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac
    23161 ataagcgatt ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc
    23221 cttttcttct gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc
    23281 agataaaatc attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag
    23341 tttgccagaa acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg
    23401 cagcatattt tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc
    23461 cgatcagcga taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga
    23521 tatcgaccat tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta
    23581 acaaagaaga tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata
    23641 acagcatgat gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg
    23701 gtaaataagt ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat
    23761 caaaaaattc gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt
    23821 ttggttcatt cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta
    23881 ctttagacgt gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac
    23941 caatcacaag ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat
    24001 tgccaaatgc gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca
    24061 taaaagcaaa cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta
    24121 cgatgccgca ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga
    24181 agatacgatt ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg
    24241 cgcctgagat aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga
    24301 aaatcgtact catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt
    24361 ttatttctag ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc
    24421 tgtaaatttt tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta
    24481 ttcccattaa attaaggtct catacatatc acttctatca taaaagggag aataaaaatg
    24541 aaacaaacca ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc
    24601 atttatcttt cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa
    24661 cttctcacac attatgatat tccggcactt catttggatg catttgaaac accagaagta
    24721 gctagtaaat ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa
    24781 gtctttcggc aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg
    24841 acagcagaag atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc
    24901 aaacagaaaa aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt
    24961 atttttattc tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca
    25021 aggtttgcaa ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca
    25081 atcgagcagc ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta
    25141 cttcttgtgc tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga
    25201 tttgttctga catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga
    25261 tttcctcatt cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt
    25321 attttagtaa agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat
    25381 cgtcgtttaa tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac
    25441 gttttcctaa ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa
    25501 cggcttcact taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta
    25561 aatagcgata atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca
    25621 cgaaatgacc tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt
    25681 ataggaaaaa atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat
    25741 ttatcatttc tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa
    25801 tagtttttta tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca
    25861 agccaatcag attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga
    25921 aatcagtaca ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt
    25981 attaaagatc attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac
    26041 agcgactctc ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa
    26101 cgagatgctg aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga
    26161 gattttcatt agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata
    26221 cgaccggctg caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg
    26281 ttccgtattg catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt
    26341 atcaggcgga cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat
    26401 tttgattcta gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa
    26461 ttatctgcaa agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga
    26521 taaagtagtc aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa
    26581 ctattcgaaa tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga
    26641 aaagcagcaa acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc
    26701 ctctaccaca aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga
    26761 tcgtcctcaa ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg
    26821 aaatgtagtc ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga
    26881 accaatcaat ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat
    26941 cgggaaatcg acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga
    27001 aacgctaggg acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac
    27061 aaacaagacg atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat
    27121 ccgaagtgct ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct
    27181 gctaagtggc ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa
    27241 ttttttgatt ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga
    27301 aaatgcattg atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat
    27361 caatcggatc gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg
    27421 agactatgat tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc
    27481 acaagatgag ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa
    27541 agaacagcaa aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat
    27601 ggcgcggatc gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct
    27661 ggaagatcac gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca
    27721 agaaacaaaa ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta
    27781 acgaaaaaac caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc
    27841 aaacaaaaat tccatattta cctttttcac caataagaag accaattact agtcattctc
    27901 actaaagctt ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt
    27961 ttgggcttat tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat
    28021 ctttctttca attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt
    28081 ttgtctaata gacctgattc tattaattct aataatccac aaaccatgat caccatacta
    28141 gatgtatctt tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta
    28201 tcttctggca accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt
    28261 tgttctttca aataacgata acttaatgca aaaccgtaaa tcccctatgc ttggcctcta
    28321 gcccaacagg aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct
    28381 ttgacatcca tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct
    28441 gcattttgaa tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc
    28501 gcccaataca ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa
    28561 ggatcactag gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt
    28621 aatgtttctg cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa
    28681 tctgctacgg ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga
    28741 cagtcaatcc tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga
    28801 tcacccgtct tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca
    28861 atattgtctc ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga
    28921 ctgttactga tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta
    28981 ttccattctt tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt
    29041 atctcttcaa acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc
    29101 tttttttatt gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg
    29161 gacgattgtt ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc
    29221 cgagccaatg ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc
    29281 atgtcatcaa gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat
    29341 cgtcacttaa acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt
    29401 cttcttctat gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat
    29461 atagaacata ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag
    29521 gaataagcca agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct
    29581 ctttcttaca tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga
    29641 tgctctcatt cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg
    29701 aatccattgc catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac
    29761 tagaataagc aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt
    29821 gtcccgcatt caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg
    29881 aaagaagttt agttccttca cgaataggca taggcatttc tttagccgtc caatactcat
    29941 ggtcttcagg tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac
    30001 tattataggg ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt
    30061 tttcatcaaa tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga
    30121 tttcacctaa tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa
    30181 ctgcaaaacg ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa
    30241 aatgaatata gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct
    30301 tttctggttc tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat
    30361 aatcttggcg acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta
    30421 tattatcaaa tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa
    30481 ttgttccgac aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa
    30541 tttgcgagaa ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata
    30601 attgttcatg atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc
    30661 tttcatgtat ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg
    30721 atagaattaa tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata
    30781 aaacccgaga gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta
    30841 tataatcaat ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt
    30901 taaataagtc aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat
    30961 tttctcctct ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc
    31021 aataaaaaaa taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc
    31081 ttctgctccg cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc
    31141 tgtcttttgg agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac
    31201 atatcctgct aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt
    31261 cgaatctttt acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat
    31321 aaaatttact gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata
    31381 acttaacgcg aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt
    31441 gtatccttgc ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat
    31501 atgcccggtt gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg
    31561 agcaatttta gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa
    31621 attcatcata ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata
    31681 gttccctgaa atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg
    31741 actatgtttg ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc
    31801 tacatcatga tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg
    31861 tattgctgcc tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc
    31921 actccaaaaa ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt
    31981 tccgttgtaa gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt
    32041 ttcagaagct acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat
    32101 cccgccattc ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta
    32161 acattcgtta ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact
    32221 gcgcccacac ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg
    32281 cgccataatt aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa
    32341 ccgtatattt atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc
    32401 ccataaagat aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt
    32461 tcgtaaaaat cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag
    32521 gaaattgact gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga
    32581 tgatcacacc tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa
    32641 tcaatgtggt aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat
    32701 tccccataaa acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta
    32761 aaactgtact gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc
    32821 tcgccatgat ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc
    32881 cacttaaaat ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca
    32941 aaaatattaa ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt
    33001 gtttagtcat ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca
    33061 aacaccatcg tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt
    33121 ttatataaat tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag
    33181 aatatatttg ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt
    33241 aaaatgattg gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct
    33301 ccatctattt cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat
    33361 aaaatagtgt tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc
    33421 caaaaaggat ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg
    33481 aataacccag tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta
    33541 atggttggaa tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc
    33601 aataaaacag caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata
    33661 atcaaaacat tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg
    33721 tcaaatccaa caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata
    33781 atgatgggaa tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa
    33841 gcactttttt tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt
    33901 ggtaacgaag attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt
    33961 tacgccgtac ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc
    34021 tgtttgccaa tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc
    34081 tggtaaaaca gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg
    34141 tcctccttag tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga
    34201 tgtagccacg cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt
    34261 aagaaattgt tgtgccactt gtggattttt ggcatcttta gggacataaa aacttgatcc
    34321 tcctgatact gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg
    34381 ccatttgtca gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc
    34441 aacaaacatt gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac
    34501 aatatctgag tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt
    34561 cagacggata tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat
    34621 aaacaattct tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt
    34681 taatgctttt gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc
    34741 tatttcagca aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca
    34801 aggaataatc gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa
    34861 ctgatcgcca tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa
    34921 tttttccagc acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc
    34981 gtgtgttacc aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac
    35041 aatttttaag tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac
    35101 atagtttgtc agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat
    35161 tggatctgcg atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa
    35221 taatagggta atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc
    35281 ttctttcttt tcaagttctt atataaaacg ctttcactta tgtatattaa cggaattagt
    35341 aacacaaata tttatataaa aaaatatttt acttgtcgtt ttggcttttc atttttgtta
    35401 taatagtcat taattataaa tagaaagctt tttatataaa aagagaaagg aaagtggttc
    35461 ttttgttaac aaacaaccaa tcctccaatc ttgtcaaaaa aacactaacc tattccatag
    35521 atacccatct tcatttatct gctgctccct attttcgact actaataaat gaaagcgcta
    35581 atttatatgc tgaatttgca cataaatcta tagaactaga taatccttcc cttttattca
    35641 ttgataaaca gcaagaagtc caaatctttt ctactcaaaa ggaaacacaa acttttatca
    35701 ttatccagtt ttattttgat gagcaattat tagcacatat tacgggacaa tctttttcta
    35761 tagatcaaga aataacatct atcattactt ttctgaaaaa tgaacagttc aaaattgatt
    35821 atctaaaaca tagtaagcca gctatttcct atgatcagga aatcaattct tttttcatac
    35881 tatgccaaca gatgagcatt catttgcttt ccttattagt tacttattgt ctgcaaaaaa
    35941 ttcaattgtc ttccttacca aggcattatc aacaggaact tgctttattg acaatacctt
    36001 caaatgagaa agtcattcct aaaaaaaagc atattcttta ttcagatgaa ttagttgatc
    36061 caatcatacg atatttagaa gaacatcttt cagaaaattt tttgcttgaa caattagccc
    36121 aaattttttt tatcagtcca gccagcttaa aaaaacattt tagaaaagtg aatggaaaaa
    36181 gtgttattag ctattttaaa gaactaaaaa tggaaaaagc aaaacgtctc gtgctagaaa
    36241 atgaattaac ctatacagaa attgcagata tattaggatt tcattcgatc catcattttt
    36301 cttcagcttt taaaaaatat acagggttat ccccttcaaa gtattatcta tccttaaaat
    36361 agaaaaaaat ttttaggttt tatccacaaa ttatttctct atattttctg tctattcaaa
    36421 ataaacaata attttccggc acaattattt gctgtttttt accataacat agtacgatca
    36481 acgtagaaaa ggagatgttt ttatgaaagt tgttgtcatt ggcggtgtag ctggcggtcc
    36541 ttcatttgcc actcgtttcc gtcgattgaa tgaagcgcaa atcattatct atgaacgcgg
    36601 agaaaatatt tcttatgcaa gttgtgcttt gccttattat ttaggaggtg tgatcactga
    36661 ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa acaaaaaaca acatcgatgt
    36721 ctttactaaa cacgaagtaa cagcaatcga tccttctact aagcgattaa cagttaaaga
    36781 cctatccaca aatgaagaaa taaaaacaga ttacgataag ttgatcatct cttctggtgc
    36841 tagaccagat tatccggata ttcccggagt ttttgaagca gaaaacggct ttgtcctccg
    36901 tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt
    36961 cgtcattctt ggtgcaggtg ttatgggtct ggaattagct gagaatttca agcatcgcgg
    37021 cttagacgtg actttaatcg atcaattgcc acaagtcgct ttcccttatg atccagaaat
    37081 tgctaattta gtttatgaca aattgctgaa agaaggatta gccgttcatt tagaaacaag
    37141 agttactgag atccgtgata aaggtcgaga aatagtatta tcagatggct ccgtcctttc
    37201 tgctgatatg ctaatttttg ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac
    37261 cggcatacaa ttatcagata caggtcagat cattgttgat gaccagttac aaaccaatct
    37321 gccggacatc tatgcgattg gcgatattat cgaaacgact agtgtagtga ctggtcagcc
    37381 gatccaaagt atgctttcca gtgcggccaa tcgtcaagga catatgctgg cagatatttt
    37441 aaatggtacg cctatgcgct atcgcggata tattggtgca ggtgtcgcaa aaatctttga
    37501 tcatacagca agttatgcgg gaatgacaga acatgaatta aaagcatcag gcataacaaa
    37561 ttataaaact gtttttatca ctccctttga ccatgcctat ttctatccag aagctacaag
    37621 attaaatcta aagctgattt ttgatgcaga tagcggtcgt attttaggtg gacaagcatt
    37681 tggagaaaaa ggtgtcgata aacggatggg agaactttct gtagcaatca ccggaaactt
    37741 gacagtcttt gatttgcctg atttggagtt gccttactct ccaccgtatt ctactacccg
    37801 tgacccgttg aatatagctg gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac
    37861 gatcaaagca agtgatatac ccgaaaacga tttgaaagaa gcgttctttt tagatatacg
    37921 tgaacctaat aaagcacctt ctggcagtat ctcggcaaca aaaaatattc caatgaatga
    37981 gttacgagat cgaatcaatg aaatcccaaa agataaaaaa gtttacatta ctttcagaag
    38041 aggattgaat acttatactt ctgcccgaat cttggcaggt ttgggtatca aagcgatttt
    38101 gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg tctaggagtt atttttactt
    38161 gaggagtaaa aatgaaaaag tatatagggt tgttggttgg tatgtatata gaatagaaga
    38221 taaatgtgaa gaaattgtgc attatttctt cacatttatt aggttatagt ttaagaaagt
    38281 ttaaacattg cgcaatttaa ttattttagc tgtttctttt cataaaaaaa catgttgtga
    38341 ctctgagaca aatcatagtc catagtgatg tctctagagt aactacgcct aatattacga
    38401 ggcatcatat taatctatca gcactagtac ctaaactagc atccctttcg aatatttttt
    38461 cttatataat aaagtttgct ttcactcctc taatctcacc aaatcgactg gcttgcgttg
    38521 tgtgatacta ggaactgatt ggtttagaac gacttttcta ttgtaatcat atttttcgcc
    38581 atcctcgttg tcttctaatc cttcaatgaa tgatccctaa tgtgtgtatt aaataattta
    38641 atttttttgt tcctatttat ttaa
//
LOCUS       DACEOM010000022        38015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEOM010000022 DACEOM010000000
VERSION     DACEOM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.023"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(356..1666)
                     /locus_tag="IW781_001068"
     CDS             complement(356..1666)
                     /locus_tag="IW781_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1182078.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1678..3096)
                     /locus_tag="IW781_001069"
     CDS             complement(1678..3096)
                     /locus_tag="IW781_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1182079.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3336..4904)
                     /locus_tag="IW781_001070"
     CDS             complement(3336..4904)
                     /locus_tag="IW781_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1182080.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4908..5318)
                     /locus_tag="IW781_001071"
     CDS             complement(4908..5318)
                     /locus_tag="IW781_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1182081.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5315..6238)
                     /locus_tag="IW781_001072"
     CDS             complement(5315..6238)
                     /locus_tag="IW781_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1182082.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6235..7113)
                     /locus_tag="IW781_001073"
     CDS             complement(6235..7113)
                     /locus_tag="IW781_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1182083.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7100..8437)
                     /locus_tag="IW781_001074"
     CDS             complement(7100..8437)
                     /locus_tag="IW781_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1182084.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8440..9543)
                     /locus_tag="IW781_001075"
     CDS             complement(8440..9543)
                     /locus_tag="IW781_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1182085.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9617..11125)
                     /locus_tag="IW781_001076"
     CDS             complement(9617..11125)
                     /locus_tag="IW781_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1182086.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11091..11783)
                     /locus_tag="IW781_001077"
     CDS             complement(11091..11783)
                     /locus_tag="IW781_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1182087.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11805..12779)
                     /locus_tag="IW781_001078"
     CDS             complement(11805..12779)
                     /locus_tag="IW781_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1182088.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13079..13951
                     /locus_tag="IW781_001079"
     CDS             13079..13951
                     /locus_tag="IW781_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1182089.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14409..16607)
                     /locus_tag="IW781_001080"
     CDS             complement(14409..16607)
                     /locus_tag="IW781_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1182090.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16839..17831
                     /locus_tag="IW781_001081"
     CDS             16839..17831
                     /locus_tag="IW781_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1182091.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17891..19312
                     /locus_tag="IW781_001082"
     CDS             17891..19312
                     /locus_tag="IW781_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1182092.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19305..20804
                     /locus_tag="IW781_001083"
     CDS             19305..20804
                     /locus_tag="IW781_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1182093.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20843..22237
                     /gene="uxaC"
                     /locus_tag="IW781_001084"
     CDS             20843..22237
                     /gene="uxaC"
                     /locus_tag="IW781_001084"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1182094.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22396..23079
                     /locus_tag="IW781_001085"
     CDS             22396..23079
                     /locus_tag="IW781_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1182095.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23081..23917
                     /locus_tag="IW781_001086"
     CDS             23081..23917
                     /locus_tag="IW781_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1182096.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23928..25682
                     /locus_tag="IW781_001087"
     CDS             23928..25682
                     /locus_tag="IW781_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182097.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25958..26299)
                     /locus_tag="IW781_001088"
     CDS             complement(25958..26299)
                     /locus_tag="IW781_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1182098.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26367..28538)
                     /locus_tag="IW781_001089"
     CDS             complement(26367..28538)
                     /locus_tag="IW781_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1182099.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28749..29606
                     /locus_tag="IW781_001090"
     CDS             28749..29606
                     /locus_tag="IW781_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1182100.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29677..30534)
                     /locus_tag="IW781_001091"
     CDS             complement(29677..30534)
                     /locus_tag="IW781_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1182101.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30519..33221)
                     /locus_tag="IW781_001092"
     CDS             complement(30519..33221)
                     /locus_tag="IW781_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1182102.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33234..34523)
                     /locus_tag="IW781_001093"
     CDS             complement(33234..34523)
                     /locus_tag="IW781_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1182103.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34673..35719
                     /locus_tag="IW781_001094"
     CDS             34673..35719
                     /locus_tag="IW781_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1182104.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35721..37874
                     /locus_tag="IW781_001095"
     CDS             35721..37874
                     /locus_tag="IW781_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1182105.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11226 a         7820 c         6752 g        12217 t
ORIGIN      
        1 atttttggct gccgtagcaa cttttatatc atatcagaga gacaaacgat tgtcaactct
       61 tttttaaaag tttttttcga tttcgttttt cgaaatcttt cagcaactca tttatcataa
      121 cttatcgttt gttatttgtc aagaactttt ttgaagtttt tttcgataga atcaagcttc
      181 gcaagtggtt ctttttaacg acttagttat cttatcacgt ctaaaaacat gtgtcaactc
      241 ttttttggaa ttttcataaa atgtttcaat gaagcgcgac aacgttgttt aatatatcat
      301 taaaaaaaat aaaggtcaag ctaaaatatt atttaagctt gaccttatta taatttcaac
      361 gattgtcaaa agtattgttt gcagctgtgt tgccagtttc tatagataaa tcacagcttt
      421 tgaaacataa gttttgtact gcttcgattc gcccgaattc ttttgcagtt atttggcaat
      481 gagaaaagca gacatcttct ataggctttt caggaaatgc tactaaatca aaagctcggg
      541 aaggaagtga gtaacctgga gaaaggcatg cctgtacgtt tttgatgtgt atgtctttga
      601 ctttcgtcat ttgctttgat tcaggtattt cttcagccac tgcttgccaa ctttctggca
      661 tttgagcaag gtcattgcta ttttttctat tgtattcatt atgccagtcc attatccaag
      721 aaaaaggaaa ttgtacatca atcatattca gattttctac cctgatattt tcgatcactc
      781 cccctctttc ttttgaagat ttcattcgaa taccacagtc tgactgaaaa aaatcgatat
      841 cgtgaatata tacgtcacta actccagcac taacttcgct accaatagtt acaccgtatc
      901 ctgaacggat tttacatcta gcaacctcga ttcttgatga ttttcgattg actcttgcac
      961 cattgccatc tctgccagac ttaatagcaa tacaatcatc accacaggcc aaatcacatt
     1021 catagacacg cacatttgtt gaagaatcaa tatcaatccc atctgtactt ggtccatcat
     1081 tgttgcggat gattactttt tctactaata catcgttaga ataagtgatt tgcaagttcc
     1141 aaaaacctga tttctgaaaa atcaaatcac gaacaacaac atgttctgca tgatacagta
     1201 aacaagcgcg tggacgttta atagcataat ccgcaatcca acgaagtccc ttttgatcgt
     1261 attcagctct tgtcccgctt ttttgatctt ttccccaata cagctcccac caatggtctc
     1321 cttgaccgtc gattattcct tttccttcta taagaatatc tttcgccgag agaacattca
     1381 aaatcgctgc tggccatttc atttctacgc cggctactcg tgtatcgatt tctggaaatg
     1441 cttcgatatc tgttctcccc ttgaggactg caccttcttc aaagataaga tgactcttat
     1501 cttttaaaaa aagggctccg actaaataaa tgccttttgg aataaataca ggtaagtgtt
     1561 ctttcgcacc ttgatcgatt gctttttgca gtattattgt atcatcttgt tgagtgtcgt
     1621 ttgttttaaa gttcaaagca ttgataatcc ctttttttgt ccgcaatacg tttctcccta
     1681 tctttcattg atttttttca attcttcacc aataaataca gtttttgaac gattcagtgg
     1741 gtaacctact agcaaaatga ttgcggatac aaataagcag aatgctggta acaagtttgc
     1801 catcgtataa atcctttctt gtactaaaac tgattggatg gctccacctg ttgtagacga
     1861 ttggtaacca atgagtgcca gcataaatcc gcctaccgct cctgcacatg cttgtcctac
     1921 ttttctcgca aaagaattta ctccatatac tgttccatct tctcttaagc ctgtcttata
     1981 ttgatgataa tcaatcacgt cagtaatgaa tgcccagacc atcaaattga ataaaccaga
     2041 tcctaaagta gcgataaata aaagaatcag atatacccat gcatttgtta tatgaaggaa
     2101 atacatcagc aagtacatac atgaagaagc aagtaacgca aatacgcttg cttccttttt
     2161 accaaataat tttgtcagct ttgtagcaaa tggtgctaaa actactactg ttccgtaagt
     2221 aaataaaaga gcaacagaca ttgcttgttt attatggaag taatcattga ataaataagt
     2281 catattcgtc ccagctaaaa tctgattgat cacaatgaaa atgtcaacaa tgactaaaat
     2341 aatcagtgct cgattagcta acaaaccttt taccaactga gaagcagata ctttttcgtt
     2401 tttgacaact cttactcttt cagtagtcaa tttacaagtt aacatatatg cgacaaatgc
     2461 tattgcagca catccgattg aaatccaaaa gaaatgattc cctgaaacga cttgttgtcc
     2521 actcttgtta tgggtataca ttaaaatcgg aatgaagaat cctgtagttg atccgccaat
     2581 cgcagaacca atgcttcgga acgtggacaa cgaggtgcgg tcattaggat ttgagctaat
     2641 tgctgctgcc attgctccat acgggatatt gattgttgag agaaaaatcc cataaacaat
     2701 gtatgaaaca aaaatataag caatcttcac cgtcattgga agatgttgga cgaatggcaa
     2761 aaacaaaatg acagtcacta agcaaaatgg atacttcatt tttctgatcc aaggaataaa
     2821 tcggccagtt tctgttaact ctgagacatc acataatctt ccaaccatca catcagcaaa
     2881 cgcatccaaa attctcgaaa tcaagaataa cgtacctacg attgcccctg aaacgccaag
     2941 aacattcgta taataaatca tcaagaaact attgactaaa cctaaaataa aacagttacc
     3001 tagatcaccg aacatgtagc ctatcttatc ggaaatccca aattcctttt tttctttttt
     3061 tattgtattt gctgtaacag cattttgtga aagcatttga attcctccta cctcttctag
     3121 taaacgtttt caaaattata actctttttt tcttgttttt atattcacaa tgttggcttt
     3181 ttattgcatt atcttgacag ataaaaaaca aaaaataatc taaaaacatt gatgcataag
     3241 attgataact ttttttacat aacaatatat acaaacaaaa aaattcacta actagtgaaa
     3301 ataagttagt gaacctcatt tattaaagaa ttaaattaca gatgatcttt tccatcatac
     3361 ttgaaaaact caaaatcagc ataacttcct ctttttttat ttagatcatg cactgctatc
     3421 ccgataaaat ttccagtaaa ccctcctgag agtatacaca tgtttttttg cggacctact
     3481 ttcttccatt cttcagttga tacgtctttc aaatgaaact gtgctgtctc tttctttaca
     3541 gtgactttca aaaaagtctt cttttctgtg agtggaatcg tccaatcttc tatttgtgct
     3601 actccgtttt ctgattctat catacgtagg cattttccct tgtcctcatt ccaagtaaca
     3661 taacaataaa tataattgga ttcatttaag tacagtacca gtccagccat ctgattaaaa
     3721 ttattaggca tgtattcaaa tgctgtctcc gcatcaaaag taaaatccgt ttgccgaata
     3781 gctaacaaat gatgttcgaa ggtagattga agagattcac cggaaatcag acgtaaaaaa
     3841 cctggtctac attttgtatc acaccaatca gcatttggca aaatgcgtct ggcattccag
     3901 ttcttcttta gctctccttc aaatgtatcc ataaactgat gatctttttc ttgttgtacc
     3961 tcttcatttg tctcaatcat tatttcttcc atcggagttg tctggctatc tttaagtctt
     4021 aaccaatcgt cggacgtcca atacacttct tgaatagcgg tttctcttcc caaaatagcc
     4081 gcaccgttta atggtctggt acaaagatat gtcatgtacc atcttcctga cggggtttgt
     4141 actaagctcc catgtcctgt acattgcaat gtagaagcag gtttgtcact agctgtcagc
     4201 ataggaaaac caggatcaag ctcgtaagga ccagtaatat tctttgctcg gcaaactgta
     4261 actgaatgtc ctcttcctgt accgccttct gccgtcatca aataataata cccgttacga
     4321 aaatacaaat gtggtgcctc cgttttcgct aattctgtac ctgcaaatat tttatagatg
     4381 ggaccatcaa gcttcttctt ctctgcatca tattcttgta atacgatacc gctggattta
     4441 ttcggtgtgt ccagacgata atcccatagt tcattgacaa gccatttttt tcccgtttca
     4501 agatcatgga acaaagaagg atcaaagccg ttactattta aataaattgg ttcggaccaa
     4561 ggacctgtga tctcagatgc agtgatcaaa taattgtgga catctttaaa cggacggcct
     4621 gcacttttga catctgtgta aacaagataa aacaatccat cagcataact gatctgtggc
     4681 gcccatatac ttccatttgt aggatttcct tgcaaagata cttgagtggt caaaatatcc
     4741 gttgtatgtg accagttgac taaatttttc gactcatata ctcggattcc tggaagccat
     4801 tcaaaagagg agacaataat gtaataccgc tctttcactc ttaatatatt aggatctgga
     4861 ttgaatcctc ttaaaatagg atttttcact ttgatagtct gcatgattca ggctcctttt
     4921 ctcattctta ctcgatataa actgaatgcc ccagctgcga aataaaaaaa tgcaaaagga
     4981 aacgcaataa actgagtgcc aatcaacaat atgaatcctg ttataaaata caaaccagtg
     5041 gcagttgtta agtattttcc tttagcagtc aaaaacaatg ctactgctaa aaaagcaagt
     5101 gaaataaaca acacaacggc aaacagacca gtcatgtcct gtaataatcc aaatgtattt
     5161 tctaaagaca aagttgaatg atctacagca agcgactgat aacttttttt aaactctgac
     5221 gcagacatat tacttaaagt aactgctaac ccgccaaaga aaatcaaaga tagactgcca
     5281 ccgaccattc ctagcaatag ttcccatttt cttttcattt ctgaaccccc tgcttttcta
     5341 aatgtatcca ttcaggtctt ttgatcgatg tttcataatt gttattacgt tcagagaatt
     5401 tgacagtctg ttcatttttc ttgtcatccc aattatccca accagttggg tgaacatgcc
     5461 cacctatctt acacgcttca aataagacat ttgcatacgg acgccacggt cttcctaaat
     5521 agacgtttct agtatcaggt tctgcagtca aggtacagct ttcaaaacga taccctgttt
     5581 tttgattttt cggcgtagat gctgctgtga tgtatccagg atttttactg gctaagctat
     5641 ggatatgaca ctgatgaaaa acggcatctg ccccaccaaa tatgaaatcc accgttcctt
     5701 caatccagca atattcatag gtctgacgac aatactcagg attggcagaa gccggtgtac
     5761 aaaaaggagt accatctttt tgcaaatcag gcaacggacc cgtgcataag gtatcctgat
     5821 aaccagatag tcgacagttt tttaaagata cgcgatctcc tgaattgaat aaagcaacag
     5881 cctgtcctac cttttctcct ggacctgctg tattttcgat gtgaagattt tctaatacga
     5941 cattcgatcc ttctaagaaa aaagtagcac ttcggaaagt ccctaactgg ctaccgtcaa
     6001 gatactcctg ttttgcaaaa cgattaccgg agataactac tgttcctgct cccacaagtt
     6061 taaaattgga taatctgctt tctatatatt catcatatct tccttctttt actatcaaga
     6121 cctttggatg gctatccggc aggcctgcca aataatcgat tccttcttgg atagtttcaa
     6181 aatccgtcgg ggtatctata gcattctttc caattatcag tttgtctttc atcctcataa
     6241 atgctccctt atgaattgtt tccaaatatt tctcatttct tgtgtttcca tatgaccgcc
     6301 tgttaaagaa aaagaagaaa agttttctgg actacccatt gattgatact ttttgtccag
     6361 cccttttctc agcttcagta cgccttcgtc caagcacatc gtatcgtttt ttccaactaa
     6421 gctaagacga gggcgcggag cgattttttc ttggattgct aacgttgtat aagctgtcaa
     6481 tagatttggc acatagtaat aaaacccatg atgatctagg cggcgatgta gcaaaagtat
     6541 ttcgatgtcc acctgtgccg ctagatcaat acaaactttg acacgagaat ccaatgctgt
     6601 cacccaccag cttaataagc ctcccataga aagaccaatc gttgcgagcc gttgagtatc
     6661 tgtgtcttcc cttgtctcca aataatcgat caagctgatc acatcataaa tacgcattcc
     6721 ccataacgtt tttcctgtta ataaaaattg tttgaacaac tcgctttcag aaatacctcc
     6781 gcgttcttca aatccccaag catcaatcga ccaaacagca tatcccattc ctgtcagtac
     6841 ttctgcaaaa gatggagaag ctaagtaagg agcaccatgg atcaattcac ttttcccgac
     6901 tgaaaaatct ccgccgtgag aatgcaggta aaggaccggc ggaaacggtc cttctttttc
     6961 tggataagca tagagggctg gtactgtttc caattcattt aagtacaatt gatagtattc
     7021 aagcataaaa ccgttttgtt tttctcggta taatcgtttt ccactagggg tatgtctgct
     7081 agatggagca cctaaaaaat cattcaactg cataagcaaa gtttttacga acaaccagtt
     7141 catccatatt ttctgattga tttgattgga atttctcaat actgatttct ttagtatttt
     7201 ctaatagaaa tgctggtccc tcgtgatttt caatcgacat ctggttaaag gaaaccttac
     7261 ccgcaaagcc aatataaaat ccttgtctgc tcatttcctc tattcctgtc atcatagcag
     7321 gaacaccggg aactgcatct tgggccatcg agacatcgat attgtcaaat gtgatttctt
     7381 gtatgaattg ttccgctaat ccataaataa aacctgcaga agcatggaca ttccttgccg
     7441 tgatattcga aaaatggatc cttctgaatg ctggtgtccg ttcatctatc ggataagcct
     7501 ttttttccca tacatacggt tctttccctc gcggaccaca gaaataataa agattcagta
     7561 taaatggaca catcacatta tccatgacta tattggatac tcgaatatct tcaacgatcc
     7621 cgcctcgacc tcgtctagat ttcaatcgta ttcctcgatc agtttcttgg aagatacaat
     7681 ttgaaatggt aatattacga atactaccac tcatttcact tcctaatacg acaccgccat
     7741 gtccatggac catcgtgcaa tttgtgatcg tgatattttc gcaagcgatc ctttcgtacg
     7801 tatcttcagt ccctgccttg attgcaatac agtcatctcc cacgtcaata tggcaattac
     7861 taatacgcac atttttgcat gattctggat caattccgtc tgtatttggt gaatccgctg
     7921 ggttcaagat cgtcaagtta tcaaacgttg cattactgca aagaatcggg ttgatcgtcc
     7981 aacttggtga ctggatcaat ttaatatctt tcacagtaat tcgatgacag ttatggaaac
     8041 tcatcaattt aggacgagga taagcgagat tatctggttc atttctaaaa gtatgccacc
     8101 atttttttcc atttccatct aatgtaccga aaccagtgac agagatgttc tctacatttt
     8161 gagcatagat acaggaagca taaacttttc gatggactcc ttcccaacgt gaatgaacaa
     8221 caggataatc ttttggatcg tcagagaatt tcagaaccgc tcctgcgctt aaatgaagtt
     8281 cgacattgct ttttaaaaat aaagctcctg ttagaaattc acctgctgga acaactacgg
     8341 tcccgcctcc gtcacttgct gcagcatcaa tggcttgttg tattgcttca gtattcaatt
     8401 catcaattga tgcgccaaat gtcaaaatat catacatatt tataatgcct ccacttcaat
     8461 caatgcttgc aagaatgcac caacgccttt ttgatcatta caaataattg gttcactgat
     8521 gtagtaagca tatgaaccat ctcgctgatc tgctccgcct aaccctgcta cttgacagtt
     8581 tttattcaag ttcagccagc cttcttttgt ttccagaaca aattcatcaa gtaatccttg
     8641 atgtgatttc ttcacttgtg gaagccattc ttctttgttc agcagtccca aacgtattcc
     8701 tttagccatt gcgtaaacaa acatactact gccggaagct tccagataat ttcctttccg
     8761 ttttgtttct gttgttactt gataccatgt cccgctgttt tcttcttgat atggaagtaa
     8821 tgcagccagt gtcttttcta gaatttcttt cagcaaagaa caatctgttt cttcatgaag
     8881 taattcatag gtatctgctg cagccatcaa ataccagcca atcgatcggc tccaaaaatg
     8941 atgggataga cctgtttttg gatcagccca tggttggacc ttcttttcat cccatgcatg
     9001 aatcagcaaa tttgtttttg gatcgaatag atgtttataa ctgatttcaa actgtctcaa
     9061 aacatcttgg taatcttttt cttcactaaa attctggata aattcagcat aaaaaggaga
     9121 acccatatat aagccatcca accaaatttg ataaggataa atttctttat gccaaaaagc
     9181 tccttcggat gtgcgtggat ggtttgccaa ctgctgggaa aggagttctg ctgctttttt
     9241 atatttttct tcgcctgttt ctttgtacaa taaaaagaaa agctttcctg tatttacatg
     9301 gtcgatattg tattcttcaa cgctgtagcc acggatcgtt ccatcttctt gcacgaaata
     9361 gtcgatattg tcttttatga aatcaaaata aattttttct tgggttcgtt cccaaagtaa
     9421 ttggaaccct ttaagtacga cgccatagtc ataagaccat tttcctttat accacttttc
     9481 ttcatataag cgaggtgttc tttccatgat actttttgct aatttctttg accagttttc
     9541 cattatttgc tctcctttaa aaattaaaat aggatttggg acaaatacca agaagtattt
     9601 gtcccaatcg tctatattac tgtgccgctg ctttgtatgc ttcctcgtac tcttgtgtga
     9661 ttcgatcgcc tccttgtgct ttccagtctt ctacagcttt tttgaatcca tcttcgtcaa
     9721 gctgtcccat gatgtattgc accgtagcat caatgacgat tttttcaatt tctgatccta
     9781 cttcattgtt cgtcggtgaa tccaatggca cagaaggatc cattacagca aattcttcat
     9841 tttctttgat caattgatta gagaattctt tttcagggtc tgtatctttg aaagagaacg
     9901 tcacttcact aggacgagag cttgataacg gttgcacctc ttgttgccaa agatccatat
     9961 cgataaattc gatagaacca tcatcattca attcataatg cgttccttcg ataccgccag
    10021 tcatcaattt atacacatct tcttctaaaa gatcatcaac aaattgaagt aatttcttca
    10081 attgtgcttc gtcttttact tgagatttgg gaaatgctag caagccgcca ataccatttc
    10141 cttctgacca aacatgataa ccgccgcctt caccatctga gatcttgttt acaggcacta
    10201 attccatatc atcttgaata ccttgaccca tcgtcttcag attcttgata tccaccattc
    10261 cagtgtagat tccagttctt ccttgtgcaa attgttcttg ctgatcagtc ttttgggtaa
    10321 ctgcaaagtc ttgtgccaaa tagccattct caaatagatc tcttgaaaaa ttcaaagctt
    10381 tcatatactc ttctgtatca aattctggag tgaatttgcc attatcgtct acagcccagc
    10441 catttggtgc tccaaaataa gaagtgaaaa gtttaaagct ggtataacga atatcactag
    10501 gcgcacgatc gccaaaacca actgtgtcgt ccacaccatt tccatcagga tcgttttctg
    10561 taaattgttt agctacttca taaagctcat ccatggtagt tggaacatct agtcctagat
    10621 tatccaacca gtcttttcga atgactaagc cagcacgagc gtagtctttt tgtatcggta
    10681 cgccgtacaa ggcaccgtcg atactcgcag cttcgattct atcttcagaa atttgactta
    10741 aatttttata gtcttttaaa tattctgata catcccagaa tacgcctgat tttaacgcat
    10801 tgcggacaga agagtttttc aacatcgcaa tttgaagtgt aacgatatct cctagttcat
    10861 cagaagccaa tgcagttgtt aatctttctt cttttgaagc atcaggaatc cagttgaatg
    10921 tcagttccgt atcggttttt tcttcgatca agttcacaat cgtgtctgta ggcggtgaag
    10981 catgatgcaa aatatttaac caagtgattt tagcttttcc atcttctgtc tctgaagaac
    11041 tgttacttga actgctgccg catgcggcaa gtgctagaat tcctgcaaaa ctaagaccta
    11101 attttacgag cgttgctttt ttcataaatt ttctccttca ctgttttaat aatatttttg
    11161 ttttcttctg ttttttctgt atatgagttc ttccattcca aatggcaaaa tatagaattt
    11221 gctgtccacg tgactgcgga aaagaagaga ctgccaccga taaaggttaa aacacctggc
    11281 attgtttgga tgatcatcag ataaatccct accatactta atatcaacac taatgagtaa
    11341 tgaagatgtg agaatccgaa taaaaatgcc attttcattt tgaaaaaata tcctttccaa
    11401 tcataatgag ccatgattgg gaaaatatag agtgctgaga gcacatatat gaacgcagca
    11461 atatacaaag cgatcctaag ataccaattg tgtatgaaag aaagatccac aagtaaaaca
    11521 atcccgatgg cgccatatat ccagctcatc agcaaggttt cacgatagtt ttctttaaaa
    11581 tatgtgaagt acgttttcgt caaaggaaac gtttcttttg agcgcagcca ttttctaatc
    11641 actgcaacca tcgcatacga tgcaggaccg aaagtaaaaa ctcccaaacc tgcgatgact
    11701 aaaccgcccc acagaaaatt caaataaacc aactgtaaaa tacgatttag ccttccgttt
    11761 attttatcta atgactgcag catatctttc cctctctttc tatcttagta aacgccgtct
    11821 tctcctagct ttttggcaat cttattagct aaaattacca taaacaatcc tacgacccct
    11881 ttaaacaatc ccattgctgt actgaaactt aactgtccat tcttcaatcc agctgtataa
    11941 atataggtgt cgaaaatttc tgcaacgctt cgattcaatg aattcaataa caagtacata
    12001 tgctcgaacc ctaagtctaa tgtctgaccg atttttagaa tcagcaaggt aatgattact
    12061 ggacgaattg ccggcaatgt aacatgccat gttttgcgta agcgtcctgc tccatccatt
    12121 tctgcagctt catacaactg tgtatcgact gcagttatcg ctgaaaggta gatgatcgta
    12181 gaccagccca actctttcca gatcacttgt ccgatatata ctgtgcgtgt ccattcaggg
    12241 gatgttagaa aactgatttt ttcaaatccc attgttgcaa ggaattcatt caatacccct
    12301 ccgtcaacat tcaaaaatac ataagtaatc gaaacaatga ttacccaaga catgaagtga
    12361 gggatgtaaa tgatcgtttg aatcccgttt ttcaatcgtt tgttcgttac ttcatttaat
    12421 agtaaagaca aaatgattgg catcggaaaa aacacgaaga tatttaatcc gaacaagatc
    12481 aacgtatttc ttaataatgt aaagaatgtc ggttctgtaa acaaacgaat gaaatgtttg
    12541 aaaccgaccc aaggacttcc cattattcct aaaaatggtt gataatcttg aaaagcaata
    12601 acgagtcccc ccataggaag atacctaaaa atcaagaaat aaaggactcc cggcagaatc
    12661 atcacatata aaaatttatt cgttttgatt cctgcaatta ttttttttcg tcttctgagc
    12721 ttttcttctt ttttcgatag cgtcagttga aatggttctt gtttctttac actttccata
    12781 agcccctcct tagttttgtg aatgtttgct gcttatgact tcatgataga taactatctg
    12841 aattacgtat ataatcattt ttaccgtaag taaaaacgct tacatagcaa tgatttttgt
    12901 gctatacaaa acgataaagt gtattttttc acaaataaac tacgcatagc aaaccttcta
    12961 tttttttcta tctgacactt agcataggaa atgattatat acgtaatctt tcaccgatga
    13021 taaattgaac atatagaaac agaaacattt aatttatttt gaggaggagc ctatctatat
    13081 gaaaaagaaa gtctctatag gagaaagaag ttttacactt ttcaacaata cgtttctaat
    13141 tttgctagct ttgatctgta tcgttccttt tttaaatatc atcgctacat cttttgcttc
    13201 tacacaagaa gtcgtcgcga aaaagtttat cctttttcct actacctttt ctttagacgc
    13261 gtatcgctac attctttcta cgccgaccat ttttagagca cttgctgttt cgattggtgt
    13321 tacaggtgtc ggaacgatcg tcagtatgtg tgcaacttca ctgatggcct acggtctttc
    13381 cagaaagtat ttgtttggtc gaggtttcgt caactttctt gttgtatttt ctatgttgtt
    13441 cagcgggggg atgattccaa cgttcttagt tgttcgttct ctagggctgg tcaattctta
    13501 ctgggccatg atcctgcctg tggctgttaa tgcaatgaat atgatcatta tgagaaactt
    13561 tttccaagcc ttgccggata gtttggaaga atcagcaaaa atggatggtt gcacagattt
    13621 tggtgtattt ttcaaaatta tgctgccatt agcgttaccg tctattgcta ctatctcgct
    13681 tttttatgct gttacttact ggaacactta tatgactgct attttgtata tcaacgattc
    13741 atctaaatgg ccgatacaga ttcttttacg ccaaatcgtc attgtatcaa gtgggatgca
    13801 agcagaaagt agtgccgttg atgtgattcc accagcacaa acagttaaaa tggctgttat
    13861 cgttattgca acagtgccta tgctgattgt ttatcctttt gttcagaaat attttgtcaa
    13921 aggagcactc gtaggttctg tcaaagggtg attatgtact gaacgcaaaa tggtagttgt
    13981 gtcagaagtg gtcagcttca agaaataaga aggcatttac gaaaattgct tcttaaattt
    14041 ttgtgaaatt tcggcttatt tttcgaagaa tctactcctg gaacaccgtg tatcagaggt
    14101 tgtgaaagag acgttcagcc ttgagcgatg ataaaaactc tcacaaacat tgcgacgagt
    14161 aacccacagg agcaataaga agcgattttg aaaaatagct tcccatattt cacaaaatta
    14221 tgccttattg tcgatagcct gtctcttgaa cgccatttat tcgtaaaaaa gaactgtgac
    14281 aagacttttg tcacagttct ttttgcgtgc ttagccttga aatttaacta tccataaata
    14341 caaatactag ggttattcta acttctttta ttgcttgaat cagtccacaa gaaaaacagc
    14401 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14461 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14521 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14581 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14641 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14701 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14761 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14821 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14881 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14941 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    15001 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    15061 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    15121 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    15181 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    15241 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15301 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15361 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15421 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15481 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15541 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15601 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15661 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15721 gcaaatctgt ccgaaaataa gtaggaaaga agaccaatga gtcctaccag tgttaaagac
    15781 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15841 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15901 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15961 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    16021 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    16081 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    16141 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    16201 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    16261 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16321 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16381 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16441 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16501 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16561 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16621 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16681 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16741 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16801 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16861 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16921 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16981 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    17041 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    17101 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    17161 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    17221 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    17281 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17341 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17401 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17461 atccagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17521 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17581 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17641 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17701 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17761 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17821 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17881 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17941 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    18001 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    18061 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    18121 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    18181 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    18241 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18301 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18361 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18421 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18481 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18541 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18601 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18661 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18721 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18781 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18841 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18901 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18961 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    19021 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    19081 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    19141 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    19201 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    19261 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19321 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19381 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19441 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19501 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19561 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19621 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19681 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19741 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19801 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19861 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19921 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19981 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    20041 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    20101 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    20161 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    20221 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    20281 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20341 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20401 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20461 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20521 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20581 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20641 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20701 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20761 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20821 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20881 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20941 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    21001 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    21061 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    21121 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    21181 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    21241 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21301 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21361 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21421 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21481 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21541 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21601 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcgtac
    21661 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21721 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21781 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21841 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21901 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21961 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    22021 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    22081 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    22141 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    22201 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    22261 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22321 gaccaaattt gctccgtctt cttctcttgc tgttctgctt cattgcctat aaacctttat
    22381 gaaagaagag ataacatgcc tactatccat attgccggtg attctactgc ggcagaaaaa
    22441 gatgaaaaca agcgtcccga gacaggttgg ggagaaaaaa tctctgctta tttttcaccc
    22501 gagatccaga tcaataacca agcaaagaat ggacgcagca ccaaatcatt tatccaggaa
    22561 ggaaggttag cccgtctagc ttcacagttt cagccgaatg atttcttatt tgtacaattt
    22621 ggacataacg accagaaaat ccaagaagaa agaggtacgc ttcccttcgg tagctattta
    22681 aaaaatctgc agatattttt tgaatgcgca aaaaaagcga acgtccaaat ggtcttactg
    22741 acatctgtga cgcgacgaga ttacttagaa aacggaacat tgaatccaga tatacttgga
    22801 gactatccaa aagcgatgcg tgcatttgct gagaaacacc atattcctct tttagatgtg
    22861 ttctcaagat cacaagaatt gttccagacc ttctcaaaag aagagacaaa aaaattctac
    22921 ctgcatctta tacctgatac ttgtaaaaat tacccagaag gactaaaaga caatacccac
    22981 tttagtccag aaggcgctga taaagttgcc cgaataattg ttgaattgat aaaagaatcc
    23041 cgtctgcctc ttgctaatta tttacaaaaa ggagtttgag atgtttccat taaatttagg
    23101 catccgtgcc cacgatttag atgttcgaaa tcgtgaagaa atcgttcaaa aagtatcaca
    23161 gcttcacttg tcccatatcc aatttgcacc acaaaaagca tttcctgaac aacttaaatt
    23221 aactgacatg acacttggga gtgctgccta ttttggtgaa tacttccaaa aaaacaatat
    23281 cgaactttcc attttaggat gctatatcaa cctatccagc aaaaatcaag aagttcgtgc
    23341 acaagcattg gcaactttca aacagcaaat tgctctgtgc tctgcctatc aagccaagat
    23401 gattgctacg gaaaccggta gtgtctctgt cggttacaca aaagaaaatt ttactgaaga
    23461 agcttatcag atcgccagaa attctatcat tgaattagtc gctcatgcag aaaattttgg
    23521 cgtaactgtt gctgttgaag cagggatcaa tcatccaatc tataactatc agttagctaa
    23581 acgtttaatc gatgaagtag cttcaccaaa tttaaaaatc attttagatt gtgcgaattt
    23641 gatgcataaa gaaaatcatg ctgatcagga aaaagtgatc cgaggtgcct tggacaattt
    23701 acatggttat attacagcgc ttcatctcaa agactatatc atggaaaatg gaaagatccg
    23761 catggtacct gttggcaagg gctgtttaga tttccgcccg gtactgcatt atatcaaata
    23821 tgagcgtcct ctaatgtacg ccactcttga agctactcct gagatttatg ttcctgaagc
    23881 aatagaacac ttgcagttta tttaccaaga ttgttaagga gaagattatg gaggaacgaa
    23941 tacagaaaag ttatcaaaaa tatcatgcaa atgcttttca gacattgctg catctttatc
    24001 agggaaatta ttggcgtttt gccatgtcag gactcttttt tattatcaaa catcttccat
    24061 cttggttaat gccgatcgct attgccaatg taatcaatgc agcttcatcg aaagattctt
    24121 ctcagttgca ggccatttat ttcaacgctt ttttgatggc ttgtctgatt ggtcaaaata
    24181 tattgacgaa ttattttcat gtaaaatacc atagcacttc gatccgtcaa gtagaagctg
    24241 gactaagagc gacccttatc aagaaaatcc aagaactatc tattccttat caaaaagaat
    24301 tgcaatctgg acgaatccag tcaaagatca tccgtgatgt agaagctatc caaacgttgt
    24361 cctctcaagt atttgtcagc ggattaaata tcgctgtcaa tttattcgta gctttaggta
    24421 tcacgcttta taaaagtcct gttgttttca tgtttttctt attgactgtt cctctcgccg
    24481 cattcgttac tctctttttc cgtaaaagga tcagacagtc caactcagac ttcaggcaag
    24541 aaatggaaga gacttctgca aaagtgatcg aagtgatcga attagcaccg attgctcgag
    24601 ctcatgggtt agaaaaacac gaaacaactc gcttaaataa gcattttact caaatctatg
    24661 aaaaaggatt ccacttagat atcctgcaat ctgttttcgg atcagtcagc tgggctagtt
    24721 ttcagatatt tcagctgatt tgtttgattt tcactgggat attagcttta caagggcgca
    24781 tccaacccgg cgatgtcgtg atgtatcaga cgtatttcac gacgatcgtc aattccgttt
    24841 ctggaatgat cacgctgatt cctacgattt caaaaggcat ggaatctatc acttctattg
    24901 gcgaactgtt gattgctggc gatgtagaaa cttatcacgg taagaaaaaa atccaacatt
    24961 tggatggaaa ttttcgtttt gaggatgttt cttttcttta tccggactca ctcaaaccga
    25021 ttatcgatca tttttctcta gaggtgaaag ctggagaaac aattgctttt gttgggccat
    25081 ctggctctgg aaaatctaca ctccttaact tattgattgg atttatccag ccgacttcag
    25141 gaaaaatttt tctagatgat cagcctttgg atgaactgga tatgcgaagt gtgcgaaatt
    25201 atttagccgt tgttcctcaa accactttgt tgttctccgc ttcgatcaaa gagaacatca
    25261 cttatggctt gaaaaacgta tcaaaagaac gtttagatga agtcatcgaa gctgcgcaac
    25321 tttcttcact gattgaccag cttcctgatg gtctggatac cttagtaggc gaacatggca
    25381 acaagctgtc tggtggccaa aagcaacgta tctccattgc acgagcgttg atacgtcagc
    25441 caaaaatcat tttgctagat gaagcaacct cagctttaga taaccagtca gagaaaaaaa
    25501 tccagcaagc cctgaattac ctgacagaaa cacccactac ttttattgtg gctcatcggc
    25561 tttcaacgat caaagaggca gataaaatcg ttgtgatcga tgaaggaaaa atcgttgaaa
    25621 taggtactta cgaaaaactg ttagctcaaa aaggctattt ctaccgcttg aaaaatgaat
    25681 gacaaaaaat aggagctatg acaagacttt tgtcgtagct cctatttctg aataaacggt
    25741 gttctcctgc caactcctcg gtcacaagtc acaatagtca tcaaacatga gaagctgttt
    25801 tttgaaattg ttccttgtaa ctcagagtta aacggtaggc tcacgacctc ttggtaaatg
    25861 gtgttcaaga gacagcctat cggcaataag aagcaatttt gtcatcgctc aaggccgaac
    25921 gtctctttca caccctctgt tgtttcataa aatagtttta ttcgctgtaa aggtcgcgaa
    25981 ctggtgtcat gatgatacgg ttcaagtcat tgatgattgt actgaagcct tgctcttttt
    26041 gcatcaagtc attgattact tcttctgatt ggattttcac agccatttct tgtgctttca
    26101 ttgcatcttc gtcgctgaac tcttgtccac tcatttgttt ttcttgtaat tcgatttgca
    26161 tcgcttggaa gtctttgaac agtttgtgtg cttcttcgtt tgcttgtact ttctcgtacg
    26221 cttctttcaa cgctttgaat tctggtaatt cacggatctc acgttcgatt tgattcgcgc
    26281 tatcgtagat gtttgacatg ttatgccctc ttttctttta gtagttactc tctattgtaa
    26341 catggggtta ttcgttttca aaggggctac tctccttgat atttccgcca gaggtcttta
    26401 gccgcttcac caattccctg aacgccttct ttcaccatat cgccgaagtt tttcagccct
    26461 tcttttgctt gttcaccata ttgattcaag ttatcttgcc aatttccaga ctctccatta
    26521 gaatttcctg tgtttccaac ttgatctgca gctaccactt gtcctcctgt ggcataggca
    26581 tctgctacac taaatcctgt ttttgaagta tacggaagga tgccttctgc cgctgatttg
    26641 aagactttcc cgacaacatt tccacttgtc ccttccaaat agtgcagctt gctggattct
    26701 tcgtaaccca tccatgtcga aatcactacg tctggcgtgt aaccgatcat ccattggtca
    26761 tttgcttttg tggcatcgaa attagtttct gtggttcctg ttttccctgc tatcgtataa
    26821 cctgcaggat tcgcagcagc cgctgttcca tttgaaaatg taccaagaag catgctggtc
    26881 atttcatcgg ctgtttcctt tgatatgacc tgttttggtt ttttgtttgt gttatcgacg
    26941 atcaccgctc cagtcgaatc aacgattttc gtgataagat gcggcgtata catctgacca
    27001 tcattggcaa atgcactgta agcacccgcc atgatcaatg gagatactcc tttttccagt
    27061 ccaccaaggg ctaagccata atacttatca gattttgcta aaggcaggcc gaattcttct
    27121 gctttatcat atcctttttg taaaccgatc tcatgcaaaa gccatacagt aggcaaattc
    27181 aaactttgtg caacagcttg atacatcggt acttctcctt ggtatgtccc gtcaaaatta
    27241 tgcgcatcgt aataactttg cggttcgtct ttcaagatac tggaaggctt gtatccagct
    27301 tccagtgctg gtgtatatac gcttaatggt ttgatcgttg atcctggtga acgacgcatc
    27361 tgtgtagcaa agctgaatcc tctaaagaca tgatctcctc ttcgtcctac tagtgcttgt
    27421 acaccacctg tattcggatc taaagctact gaagcagatt ggaccatggc accatctgaa
    27481 gcatttgctg ggaataatgc actgttttga taagtagctt ctaattgctt ttgataatcc
    27541 tgatctaaag ctgtatagat tttatagcct ttattcatga catcttcttc atcaagacca
    27601 tactcgttaa ctgcttcatc gatcactgca tcaaaataat aaggataacg ataaccagaa
    27661 tctgaattat cgtatgtgtc attaagatac gcgctgatat ctgtctggct ttctgcttgt
    27721 tcttgttctt ttgtgatttt tccgttttct tccattacac ttagtactgt gtttttccga
    27781 tcattcgcat tttttgggtg atcgatcgga ttataaatac caggaccttt cagcattcct
    27841 gccaaagtag cagcctcaga cagcgtaaca ttcgttgcat ctactccaaa atactttctg
    27901 gaagcgtctt ggacacccca taccccgttg ccaaaatagg cattgttaag atacatggtc
    27961 aagatttctt ctttgctgta cttcttttcg atctcgattg ccaaaaataa ttccttcgct
    28021 tttcgatcaa acgtttggtc taatgtcaga tacgcatttt tggcaagctg ctgagtgatc
    28081 gtacttcctc cgccccctga tgttccgcca gacaagacca ttcggactgc tgcacgggca
    28141 atccctttga tatcaaaccc gtgatgctcg taaaaattcc tatcttctgt cgagattact
    28201 gcatcttgga tatagggaga aatcgcgttt aattctacat atgttccttt ttgggcgtac
    28261 agtgtgccag cttcttcgtt tttcttatca taaatgactg tcgattggct taatcctgat
    28321 tttaacgttt cgacatttgc ttgttttgct aaaataaaga ggtagatact tgtcactaag
    28381 acacagatca aaccaatcag caaaatcaat ttgttcacat gatatttttt ccagatgcgc
    28441 tttcgccatc gatggaattg tccgagatat ggtctgatcc aacgccaaaa actgatcaaa
    28501 gcagctttta tcttttctaa aatgctttga aaatccattt gcttcttctc ctgtctattt
    28561 gattcaatcg tcttatttta ccatagtgaa agagtgtttt cttcctgctt aagttgtagt
    28621 tcaatatgtt tttttacgtt agtgcaagta gagtaacata agaatgaaca gaaaaatgta
    28681 agttttttct taaatatgtt ttaaaaatgg tacaattagt ctgcgattgc actgaaagga
    28741 gtacacttgt gaaaatcacg atcactttac ctatgaacca tcctgaaaca acaattcgcg
    28801 acttgcttga aaaggaatgg ttagttccta gaaaggttcg acactttttg cgtatacgta
    28861 aaaacgtttg gatcaatcaa caacctgctt tgttccatca tgaagtaaag ggcaatgacc
    28921 agatcacatt gttattcgaa gaaacagatt ataattatca agaagttcaa ttaggagatg
    28981 cgaagcacat ccgtgtttta tatgaagatg agcatttgat tatagtggat aagcctgcag
    29041 gaatgaagac tcatccaaat gaaccgacag aaaacagtac cttactcaat catttagctg
    29101 cttatctcga cactaaacag caacgacctt acgttgtcca ccgcttggac aaagaaacaa
    29161 gcggtgccgt tttatttgca aaaaatccat ttgtcttgcc gatacttgga agaatgttgg
    29221 aacaaaaaat gatctatcgg agatatcaag ccatcgtatg gggagaaatt gccaaagata
    29281 tgaccatcac caataaaatt ggacgagatc gccatgatcg gagaaaaagg gtcgtagatg
    29341 ataaaaatgg tcagagcgcc ttgacacatg tcactgtcaa caaagtactt tctgaacaaa
    29401 cacaagtcta ttgtgttttg gaaaccggac gtacacacca gatcagagtt catttagctc
    29461 ataagggaca tccgatcgtt ggagaccctc tctaccaaaa tagaacagct gaacgactga
    29521 tgctccatgc attagaactg cacatgatcc atccatttac tcaagaaaaa atagtcggta
    29581 aagctttacc tggattatgg gaatagaaat aaaaaagaac agagtcttat cagcagataa
    29641 tgataagact ctgttctttt tgtgtaattt gtctgcttac ttttgatgtt cgtgttctgc
    29701 taacagcaaa ctaccgataa tcccactttc attcggcagt ccccaacgaa cgatatattc
    29761 acttacttga ggtgtttcca tataaccacc catcaattca acaaattgct gacggatttt
    29821 ttgaaggaga tgatcctgat tcattacccc accgcctaaa ataatctttt caggtgctaa
    29881 cgtcaacgta tagttgatca gtgcttgagc aatataataa gcctgaatag cccaaacagg
    29941 atgatcttgc ggcagatctt gccctttgat ccctgtgcgt gcctcaatag acggtcctgc
    30001 tgctaatcct tctaaacaat ctttatgata aggacaagta ccttcataat ggtcatctgg
    30061 atggcgtttc acccaaatat gtcccatttc aggatgtgcg atccctgaaa aaatttcttc
    30121 attgatcacg acacctgcac cgattcctgt tcccacggta aggtaaatac aactattttt
    30181 tccttgtgct gctccttgct tcaattcccc atatgcagca gcattgacat ccgttgtcca
    30241 agcagcagga atattgtagc gttccttgat tgtgccaaga aaatcgaatt gtgcccaccc
    30301 ttttttggga gttgccaaaa cgtatccgta attatctttt gttttgtcga taccaatcgg
    30361 gccgaatgag ccaatcccga ttcccttcaa ttcataacga tcaaaaaatt caaatacctg
    30421 atccaatgtt tcttctggtg ttgtggtagg gatacttaat ttttctaaaa tttctccacc
    30481 atctgaaacc gcacacacaa attttgttcc gcctgcttct attccgccat acattcgttt
    30541 tcctcctcga atccgattgt ccgaatctca taaggttgga ttattgggac aaccgcttgc
    30601 ttcgttggtt cttcaagcag attcaatatg acagtttttc cattgtcttt agttatttcc
    30661 aatttttcta aatgactaga catattgaaa cctcttacta ctactttatc actaaacttg
    30721 ctgcgtttca aagctgtaat tgcaaacgtt tcacttttta tcgttaaata ttgttgtttt
    30781 gaaatcaatg ttccttcatg atgtttgatt tgttgagtgg aaaaaggaat ctgtgctgcg
    30841 tatgcatgca aataagtaga gaatttttct tctggtccat gtagttcaat gctgtaattg
    30901 aaccgatgct cacctagaca ctgtgcttct ggtgttggaa aatatcccca atcgcctagt
    30961 tctcccacac tgcgaagtag cgtcactgcg atttgtccgt cttctgttac ttcatattca
    31021 ttcaaaccaa agtttccgac tgttacaccg tactcttcat catgaatatt gacaaatgct
    31081 tgctgatgtt gcggattcgt ggggttttcc caacttttag acacatggtt tgggcgcttc
    31141 accacttcaa aaatactatc tgcttcgtga tgttccacat gtaactttgt aggaaacaag
    31201 acacgtaagc gatgatcttt catctgattg tcgagagtcg tttcaaagtc gattttttta
    31261 ctatcttttc tgattgtcat gattgttttt atttgaagga ttcgtttttc tttggatcgt
    31321 tctgcttttc gataacgaaa ttccacaacc atctgctgtt ctttttctaa acgctcgtct
    31381 gcagaaacag ggatttccat ttcctgtaga atacttattt ttgctatttc cggtgtatct
    31441 actaatactt ttacttcaga gttttctttg ttactggata ggattggctg atcacagaaa
    31501 ggttgtttaa agatatactc atttcctata tcgccgatat cttcaaaaat atgcagatta
    31561 ttcaactttc gtttgttttt gcgatcttcc attgtaatgg taccattttt ttcaatcgtc
    31621 aaatgtagag ggccgttttc gatgattcgt ccactctgat gaatcattgt ttccttttct
    31681 tctgctttcg cttccccttc tacaagcgcg aatgtttccc aagaaaatgc tggcatgttt
    31741 tctaatggta aagtcacttt gatcattctt gccatatatg gcacacggaa acggtctttc
    31801 ggcaaatcat aaccaaattg gacagtttct tcagataaaa tagctggtat agtggcccca
    31861 ctttttgttt ctacatgata cttacgttta ggcaaatttt ctaactcttg atatagttgt
    31921 tcaggtattc cttcagcgaa tctttttctt tctaattcga tggttatttc tgcttcacca
    31981 gttttatcca ttcctgcagt atttacaatg acaaatggaa agctgtcttt tgggaatccg
    32041 gtcgtatcga ttgctgcggt cagctgttct aaggaatctt gtgcaagata ctttccaact
    32101 tcatctgctt tttcaaaacg aggtatcatt tcccgatgga cagaatcgac tgaacaaccg
    32161 caaatactgt cgtgaggatg attttgcatt aatgtcttcc aagcataatc taattgatca
    32221 tggggatagt ttccactaac ttcataagcc atcgtcgcaa gtggttctgt aatattttct
    32281 agctgtcgct ggaccttggt attccattgt tttaagtaaa cccgagcaga tgctgtattg
    32341 gctaaagtat accagccatc cgtttcttga cttgtcaatt ctccttctac tgagcctaag
    32401 tcttctggca catctttttg aactgcttcc agatagtcag taaaattaga atgaataaac
    32461 tcgtaatctg gaaacagttc atttgcgaga tggattgctt ttgatatatc tttttgtacc
    32521 ggttgatgat caacaccatt catcatcaat aagtggttcg tagaggcata ttgttctgca
    32581 tccgctaatt tttgcttcca aaaggcaaga gctgcttctt tttctactgg aatctcattc
    32641 ccgttgctat accaattggc aaatagcaga ccaaagattg ctgtttgatc aggtcctttc
    32701 caccacatct ctgagtattg ggaagaatag ttttctgctt ccaaaacttg attatcaaag
    32761 ccaattggtt ttacaccacg gccaaacgct gctgctgaaa taccagcttg tttcattaat
    32821 tgaggcgtct gtcccatatt tccaaatgta tcgggaaaat agcctaacat gacaggggtt
    32881 ccccatttac ggctttcttc catgccaatc agcatatttc tcacgttcga ttccgagctg
    32941 ataagaaaat catcttgcaa gatataaaat ggtccaattc tcaattttcc ttcattgatt
    33001 gcttgttgga ttgcctgtct tttttctggc cttacttgaa gatagtcatc taaaataatc
    33061 gtttgcccat ccaaatgaaa actgttgaag gacggatcaa cttcaaatag ctctagtaga
    33121 tcgtctatca attcaatcag tcgcatgtga tgctgttcgt aagccatgta ccattcacga
    33181 tcccagtggc tatgagaaat gatatatact ttctttttca tttacgatac ctcctacttt
    33241 tctactctaa tatcaaagta atccatgact aattcgcaaa acatcatatt tgcccaagaa
    33301 aaccattctc tcgtataatt ttcaggattg ttcacatcga atccttcgtg cataaggtgt
    33361 gttcctgcgt cagttgctac aagtaaattc aatatccgct ctttttcaga tttgtctgac
    33421 gttgtcattc cttccatagc taatgcaatc ggccaaacgt atttttctgg cgtatgggag
    33481 gatccaattc cttttgcgta ctttccttca tagaaatatg gattttcttt actcaacaat
    33541 gttcttcttg tttgcagata atcttcctct tgttcagaac aatatcccaa gtaaggtgcg
    33601 gcgatcagat tgggcacatt actgtcatcc atgactgtgg cgtttcccat tccatctact
    33661 tcatatgcat aaatcgtttg ccctttttga ttaaccgttc gtccgtgatt ctgaatcccc
    33721 tcttggatct ctttcttcaa tttctgtgtt tgctgtacaa tttctttatc atcgagtatc
    33781 gtttgaaaaa tttcttccac ataacctaac acgaccaccg caaacatatt ggatggtacc
    33841 agatatccat aacggcaagt atcatcgcta ggacggaaac cagaccatgt cattcctgta
    33901 aaagctgttt ttgcacctcg cccgtcattg atcagcgtat cttcttttct tgtcgtgtct
    33961 cgtacaaatg tataaggaga gtgttcatga tcttgctctg ttttgaacac gtccaagatt
    34021 tttttgatac cttcaataaa agattgattg aattgatcag tttttcctgt atttttgtaa
    34081 agaagatagg ccaactgtat tgggtagcat aaagaatcaa tttcatattt tctttcccag
    34141 atccaatcgt tcatttttgt atgatctgtt tggtggccag caccgttagc ttcttcgttg
    34201 aaggcattcg catatggatc gatattgata taaaaaaatt gctttttgac taacccgctg
    34261 atcatgtcag ctaattcctc atcttctttt gcaatcacta aataaggacg aacttgtgca
    34321 gtagagtctc ttaaccacat cgctggaatg tctcccgtca ataaaaaggt ggttccgtca
    34381 ttttgctttt tgactgttgt caacagagta ttggcaaatg ctgctgtaaa attctttgcc
    34441 cagtccgcat ggatatcccc acatttttgt gtgatatctt ccataaattt ctgcaccgat
    34501 tttggtatat ctttataagc cactaataaa accttctttc tatttgatat atctttaaag
    34561 attatacttc atacagccaa ttatgtctac gctttcaaaa aatatctttt tatttacttt
    34621 ttcatccatt atggtattat ttgaatatga tatatcatta agaggtgttt atatgaatca
    34681 accattgtac cagacaatat ttaatgattt gcaaacggct attttaactg gtgaattacc
    34741 tgtagacagc caactaccaa ctgaaaaaga actttcacgt acctataaag taagcagaat
    34801 cacttccaaa cgtgcattga ctgaattaga gcaattagga cttatttatc gcgttcgcgg
    34861 gaaaggcagt ttcgttaaat ccgcttctcc ggaacagatt cttgcttctc ctacaaaaac
    34921 acgacggatt ctgtttcttc ttccatatgt cgctgatttg tctgttggtg attttagcaa
    34981 gggattgaac cctgtgatgc aagataaaca gattgatgtc atgatgacga cgcttgactt
    35041 tctacaaaat aaaacagcaa aagagctaat caatgaattt gacggattga tttattatgc
    35101 tcttgataca gaacaacatc ttgacttact ttttgagctt tcattgaaag aattccctgt
    35161 cgtcatatta gataaaaaaa tctatgagtt accttttcct accgttcttt ccgataattt
    35221 ccaaggcggt gcgcttgctg cctcttattt gatcgaacag ggacattcaa aaatcgctta
    35281 tttgtttggt acgcagactc atccacaatc cgttcgacag cgttaccttg gttaccttga
    35341 agcattggac aaagctggtt tgactttcca cacttcactg gatgacaaaa gcgcgacatt
    35401 agatgaattg ctttcttata tcactaagta tgaagtaact gcttttgttt gtgaaaacga
    35461 tgttgtcgct atccagacca tgaatctctt gagacagcat ggttatcata tccctgaaga
    35521 tttttcaatt atcggatttg atgatatcca agcagcagct cttgttgacc cgccactgac
    35581 aaccatcgca caagattttg aacagttagg taagcttgcg ggagatgctt tggtcacttg
    35641 gttagagaca aaagatcgac catccgatag taaatttcca gtttcattca tcaaaagaca
    35701 atcaacaaag gagaaataaa atgaatatcg gtcaaataga tactagacat gcaacagcga
    35761 atcagccaaa tttctctaat gggaactgcc agccttatac tggcgttcct tttgggatga
    35821 attattttgc accgcaaact acggatcaaa acggcagctg gtggtttcat cctgatgacc
    35881 gcgtttttca aggctaccgt cttacccatc aaccaagtcc ttggatgggt gatttcagtc
    35941 atatgctgtt tacgccgatc aatggaaaac tacaggagaa cacccttttc catgctcaaa
    36001 gttcttaccg cccagaagaa agtatatttt gcccgactca tctgtcgatc tgtcaattac
    36061 gttatggcat ctgttctaca ttgataccta gcatgtatgg cggaatctta tctattgatt
    36121 acagcaaaaa tgacagcggt ttacttttat cctttccggg acgccatcag ctctttgtta
    36181 tagatcctta cacagtggaa ggaaaaatca cacattttgc tggttcagaa gatcctgatt
    36241 tttcttttta ttttatattg agatttgaat atcctttaac aattgatgag ttagctgttt
    36301 ccaacaaaaa tagcgactcg atcccgttgt attttgaaca gacaaaaaaa caaactatcc
    36361 gatttgggac atcttttatc agctcggaac aagcagctta taacctggaa ctagaacaaa
    36421 accaaagcaa agagacttac cttgctcaca gtcaagaaca atggcagtcc tattttgatc
    36481 gtatacagct cacacaccat gatcaacaac aattgcggac gttttatcat aatttatatc
    36541 gggtgttttt atttccacaa acattttatg aaattgatcc tagtgggcaa aaaatccact
    36601 atgatacgac tagtcgatct ataaaacctg gtgttctcta tacaaataat gggttttggg
    36661 atacgtataa aacggtttat cctctttatt ctttgattgc tgtagaaaaa tacgaagaaa
    36721 tgctggaagg ttttttgaac agctatagag aaagtggcta tttgccaaaa tggctgtctc
    36781 ctgatgaacg tggacttatg cctgggacat tgatcgatgc ggtcattgca gatgctgctg
    36841 taaaagatat ccgacgagac ttgatgcctg aatttctaac ggcaatggaa aaaggtgcta
    36901 cggttcaaag caaacatcca aattatggac gccaaggaac agaagattac ttgacatatg
    36961 gctatgttcc taatcactac cacgaatcag tcaatcacac attagactat gcttatagcg
    37021 atttttgtat tagccaagtt gccaaggtac tagaagaaga ggaaatgacg aagaaatatc
    37081 agcagcaatc attgaactat caaaatatct ttgactatga gtcaggattt atgcgttcaa
    37141 aagataaaga tggcaagttc agagaaccat tttcagctaa tcgttgggga caagattatg
    37201 cagaaggaag tgcttggcaa agcagctttg ctgtttatca cgatttttca ggactgatcg
    37261 gaaaatatgg tggcagtgag agatataaag aaaaactcat tgagctgtgc aatcagcccc
    37321 caacgtttga cccaggagga tatggttttg agatccacga aatgagtgag atggccgcga
    37381 tagaatttgg acaattagct atttcaaacc agccaagttt tcactatcct tacttgttca
    37441 gttatatagg acaacctgaa atggcacagc cactgcttaa acagttgatg actcagacct
    37501 tcgacgattc tccaatgggt taccctggag atgaggacaa tggaagtatg gccgcttggt
    37561 atatcttcaa tagtctggga ttttatcctg tcacgcctgg aaccggtgaa tatgttatcg
    37621 gtatgccatt aattgacaag gctgttttgc ttttgtcaaa tggaaaggaa ttgacgattg
    37681 aagcaacgcc taatcggccg cagcaacaat ttatccataa aatcaaacgg atggacaaag
    37741 aatatacgaa attatatttt acccatcaag atttgctgaa aggcggcacg atctcttacc
    37801 aattagggat catccctcac cctaagaaat atcaagcaaa agatttgcct ttttccttat
    37861 caaactataa ttaacaagag gttgtgaaaa agctgtcctg catcaagtaa taagaacagc
    37921 caagcaaaaa tagatcctca tatttttcgt ttggttggtc ttattaccgc agatgcaagc
    37981 ttttgaacac cgtttattga gaggttgtga aatca
//
LOCUS       DACEOM010000023        36530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEOM010000023 DACEOM010000000
VERSION     DACEOM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.024"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            525..1409
                     /locus_tag="IW781_001096"
     CDS             525..1409
                     /locus_tag="IW781_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1182106.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            1618..2196
                     /gene="pgsA"
                     /locus_tag="IW781_001097"
     CDS             1618..2196
                     /gene="pgsA"
                     /locus_tag="IW781_001097"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1182107.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2436..3677
                     /locus_tag="IW781_001098"
     CDS             2436..3677
                     /locus_tag="IW781_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1182108.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3786..4835
                     /gene="recA"
                     /locus_tag="IW781_001099"
     CDS             3786..4835
                     /gene="recA"
                     /locus_tag="IW781_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1182109.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5246..6802
                     /gene="rny"
                     /locus_tag="IW781_001100"
     CDS             5246..6802
                     /gene="rny"
                     /locus_tag="IW781_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1182110.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6881..7687)
                     /gene="proC"
                     /locus_tag="IW781_001101"
     CDS             complement(6881..7687)
                     /gene="proC"
                     /locus_tag="IW781_001101"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1182111.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7828..10401
                     /gene="mprF"
                     /locus_tag="IW781_001102"
     CDS             7828..10401
                     /gene="mprF"
                     /locus_tag="IW781_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1182112.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10555..11406
                     /locus_tag="IW781_001103"
     CDS             10555..11406
                     /locus_tag="IW781_001103"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1182113.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            11651..12589
                     /locus_tag="IW781_001104"
     CDS             11651..12589
                     /locus_tag="IW781_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1182114.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12612..13292
                     /locus_tag="IW781_001105"
     CDS             12612..13292
                     /locus_tag="IW781_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182115.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13265..14089
                     /locus_tag="IW781_001106"
     CDS             13265..14089
                     /locus_tag="IW781_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1182116.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14256..15074
                     /gene="purR"
                     /locus_tag="IW781_001107"
     CDS             14256..15074
                     /gene="purR"
                     /locus_tag="IW781_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1182117.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15577..16950
                     /gene="glmU"
                     /locus_tag="IW781_001108"
     CDS             15577..16950
                     /gene="glmU"
                     /locus_tag="IW781_001108"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1182118.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            17044..18015
                     /locus_tag="IW781_001109"
     CDS             17044..18015
                     /locus_tag="IW781_001109"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1182119.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      18021..18123
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18189..18758
                     /gene="thiT"
                     /locus_tag="IW781_001110"
     CDS             18189..18758
                     /gene="thiT"
                     /locus_tag="IW781_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1182120.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18839..19612)
                     /locus_tag="IW781_001111"
     CDS             complement(18839..19612)
                     /locus_tag="IW781_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182121.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19878..21578
                     /locus_tag="IW781_001112"
     CDS             19878..21578
                     /locus_tag="IW781_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1182122.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21548..21766
                     /locus_tag="IW781_001113"
     CDS             21548..21766
                     /locus_tag="IW781_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182123.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21786..22676
                     /locus_tag="IW781_001114"
     CDS             21786..22676
                     /locus_tag="IW781_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1182124.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22694..22978
                     /locus_tag="IW781_001115"
     CDS             22694..22978
                     /locus_tag="IW781_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182125.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23465..24079
                     /gene="gmk"
                     /locus_tag="IW781_001116"
     CDS             23465..24079
                     /gene="gmk"
                     /locus_tag="IW781_001116"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1182126.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            24084..24389
                     /locus_tag="IW781_001117"
     CDS             24084..24389
                     /locus_tag="IW781_001117"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1182127.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24642..27050
                     /gene="priA"
                     /locus_tag="IW781_001118"
     CDS             24642..27050
                     /gene="priA"
                     /locus_tag="IW781_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1182128.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            27065..27556
                     /gene="def"
                     /locus_tag="IW781_001119"
     CDS             27065..27556
                     /gene="def"
                     /locus_tag="IW781_001119"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1182129.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27549..28487
                     /locus_tag="IW781_001120"
     CDS             27549..28487
                     /locus_tag="IW781_001120"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1182130.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28487..29845
                     /gene="rsmB"
                     /locus_tag="IW781_001121"
     CDS             28487..29845
                     /gene="rsmB"
                     /locus_tag="IW781_001121"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1182131.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29858..30598
                     /locus_tag="IW781_001122"
     CDS             29858..30598
                     /locus_tag="IW781_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1182132.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30595..32664
                     /gene="pknB"
                     /locus_tag="IW781_001123"
     CDS             30595..32664
                     /gene="pknB"
                     /locus_tag="IW781_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1182133.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32829..33728
                     /gene="rsgA"
                     /locus_tag="IW781_001124"
     CDS             32829..33728
                     /gene="rsgA"
                     /locus_tag="IW781_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1182134.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33741..34391
                     /locus_tag="IW781_001125"
     CDS             33741..34391
                     /locus_tag="IW781_001125"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1182135.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34392..35030
                     /locus_tag="IW781_001126"
     CDS             34392..35030
                     /locus_tag="IW781_001126"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1182136.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            35185..36039
                     /gene="mreC"
                     /locus_tag="IW781_001127"
     CDS             35185..36039
                     /gene="mreC"
                     /locus_tag="IW781_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1182137.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            36043..>36530
                     /gene="mreD"
                     /locus_tag="IW781_001128"
     CDS             36043..>36530
                     /gene="mreD"
                     /locus_tag="IW781_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1182138.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PF"
BASE COUNT        11935 a         6486 c         7996 g        10113 t
ORIGIN      
        1 atttcggctt attgccgaag aagctacttc tggaacaccg tgtaccaaga ggttgtgaaa
       61 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt aatgttttta
      121 acttggttgg acttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg
      181 tgaaatcagc gttttgacct gagtattaga gaaacatacg gaaaaatatc tccttatatt
      241 ttgccggttt ttgagctaat accgaggaac agctgattga acaccgttta agaaagaggt
      301 cgtgagcctg ccgtttaact ccgagtcaca aggaacaagt tcaaaaaaca gcttcttata
      361 tttattggat attgtgactt gtgaccgagg agttggcagg agaacaatgt ttattcggaa
      421 aaaaggagct gtgacatgac ttttgtcaca gcacccaaac ataacgtctt tcgttgcaac
      481 agcagatgct tcggagaatc aaacagactg gagaagatga acgagtggct tttgaaacca
      541 tcggagaaaa attacggcaa gcacgcctga ataaaaaaat atctttagat gaattacagc
      601 agattacaaa aattcaaaaa cgttatttgg aagcaatcga caatgatgat tttgaccaat
      661 tgcctggtaa attttacgtg cgggcattta ttcggcaata cgctgaagct gtaggagaag
      721 atggagacca tttagtcgat gtttttgatg ggaaaagcaa attgacagga tcggtccctg
      781 aacgtccgca gcctgaaaca gtaagtactt ctagaaaagc aatgcatcag gaagaatcgc
      841 atccaagtcg cttttggaca agtcttcctg tcattttatt aggattggtt gctttggcga
      901 ttgtcgttgt cgtgggctat atgacttggc aagatcgtca agctgatcca ataatcggag
      961 acacagcttc ttcgcttaaa gtagaaggat cagtcgagga gtcctcttcc tcagagcaaa
     1021 gcacgcagac caaagagtct tctacagtag aatcaacaac aaaatcaagt gaagaaaaga
     1081 aaatggcaat agctacagga gaagattctg gcagtgccat cgcagtaaat atcacagacg
     1141 ctaccaagcc tgtaaccctt gaatttactg ctgtcaatcg tgtatggtta ggtgtgctag
     1201 tagacaatgc ctatgtttat caagggacac tggcagcaaa tgaaacacag agtaccgtgt
     1261 taccggaaac agcaacaaat gcgaccatca cgattggtgc agcaagcaat gcaacgatca
     1321 aagcgaatgg agaatccgta ccagttaatc caggggaaaa caatcagagt ccaaaaaatg
     1381 tgaaccttac tcttcaatac gcagaatgat cgtataaatt agtttacgca tctgtttact
     1441 tgtaggtata aaaaagaagg atcgtgacaa atctgtgtcg cggtccttct tcgttaattc
     1501 aaaaagatca atttcaataa aaatactcct ttgaataaat tcatttggaa aagtaaataa
     1561 tatcggtgga aacttcgctg atagtcacgt agaataggga agaaaggagt cgatagtgtg
     1621 aatttaccaa ataaattaac tgtacttaga atttgtatga tcccgatttt cattttggtt
     1681 gtaggtttac ctttagattg gggacaattt aacgtaggag acaccacact ggctgtcaca
     1741 cagctagtag gtgcagttat ttttgcttta gctagtatca cagactggtt agatggaaaa
     1801 attgcccgtg cgcgaggcct tgtcactaat tttggcaagt ttgcggaccc gttggcagat
     1861 aaaatgctag ttatgactgc ttttatcatg ttggttgacc aaggaaaggc ccctggttgg
     1921 gtagtagcga tcattgtctg tcgtgaatta gccgtcacag gcttgcgttt actacttgtc
     1981 gaacatggag aagtcatggc tgcagcttgg ccgggaaaag tcaagacggc tacacaaatg
     2041 gttgctatta tcttgctgtt tatcaataat atcccattca gcctgatcgg tattccagta
     2101 gatcaaatca tgctttatgc ttgtctgatc tttactatct attcaggtgt ggattacttc
     2161 atcaaaaaca gcggtgtgtt caaaggatca atgtaagtat cgtttagtgg agaggttgtg
     2221 agcctgccgt ctaactctga gtcacaaggg ataatttcaa aaaacagctt ttcatgtttt
     2281 atgtatattg tgacttatga ccgaggtttt ggcaggagaa cactgtttat ttggaacaag
     2341 gagctgtgac aagatttttg tcacagctcc ttgccctctt tttaaataaa aaaatataaa
     2401 aatacagcgt atactatata agggagcggg aagtaatgaa agcagaaata atagctgtcg
     2461 gaacagaact tttactagga caagtcgtga atacgaatgc cactttcttg tcagaacagc
     2521 tagctgatct aggtatcgaa gtttattatc aaacagttgt aggagataat cagcagcgtt
     2581 tagaagagtt gattgctcta gctgaaacaa gaagtgaact catactctta tgtggcggat
     2641 taggaccgac agaagatgac ctgacaaaag aagcaactgc ggctcacctc ggtaagtctc
     2701 tcatacaaaa tacggaagga tacaaaaaat tattggcata ttttgaaaca acccatcgaa
     2761 aaatgaccaa aaacaatctg caacagtccc agattatcga aggcggggtt cctctaccaa
     2821 atcgtacagg gcttgcttta gggacttttt atcaaacaga tacacatgcg tatatccttc
     2881 ttcccggtcc gcctaatgag ttgaaaccaa tgtttgtgga acaagtacgt cctttactag
     2941 aggaacgttt tccttctgaa gaaaaactaa tttctaaagt tttgcgcttt tatgggattg
     3001 gtgaatcgcg actggtcact gagctaaaag atctgattga gacacagatc aatccaacga
     3061 tcgcaccata tgccaaacca aatgaagtga cattacgact gacagtgaaa acaaatgatg
     3121 ttcaggcagg gaatcaagct cttttagcat tagaagaaaa aatccaagaa cgagtagggg
     3181 aatattttta tggatacggc gatgataatt cgctagcaaa agtcgtagtg gaactactga
     3241 aagaaaacaa gcagactgta acagcagcgg aaagtttgac agccggcgcc ttccaagccg
     3301 cattaggcga cattgctggt gtatctgaag tatttccagg cggattcgtt acctactcgc
     3361 tccaaacaaa agctggtttt ctggagattg atccggagct attagcagaa tatggaacag
     3421 tcagcaaaga atgtgtggag cagatggcca ttcaagcacg tgagatagca agaacagatt
     3481 acagtgtagc tttcaccgga gtggctggac cggacacgtt agaaggtcaa tctgcaggaa
     3541 cagtatggat cgctatttca tctaaagacg gcgtggtttc ggaaaaatat cactttgcca
     3601 gagatcgtgc tgctatcaga tataatgcgg ttatgcaagg atttgatctt ttgagaaaac
     3661 agcttttaag aaaatgataa atgcgaatat ttgttcggtt ttttcttgct ttttttcact
     3721 aaaaaagata tgataaggat aatgaaatgt tgctctgatt tgtaaatata aggaggatta
     3781 tcgtattggc agatgatcgt aaagcagcct tagatgctgc actgaaaaaa attgaaaaga
     3841 gctatggtaa aggctctatt atgaaattgg gcgaaaaaat cgaccaacaa atctctacaa
     3901 ttccaagtgg ctctcttgcg ttagatgtcg ctttaggtgt cggcggttac ccccgtggac
     3961 gtatcatcga agtatatgga cctgaaagtt caggtaaaac aacagttgca ctacacgcta
     4021 ttgcagaagt acaaaaaaat ggcggaacgg ccgctttcat tgatgctgag catgcgttag
     4081 atccgcaata tgcacaaaaa ttaggtgtga atatcgatga actacttctt tcacagcctg
     4141 acacaggaga acaaggtcta gagatcgctg atgctttagt atcaagtggg gctgtagata
     4201 tcgtagtagt cgattcagtt gctgctttag ttccacgagc agaaatcgac ggcgaaatgg
     4261 gtgactcaca tgtcgggtta caagcacgtt tgatgtctca agcattgcgt aaactctctg
     4321 gttcgatcaa caaaacaaaa acaatcgcta ttttcatcaa ccaaatccgt gaaaaagtag
     4381 gcgtgatgtt tgggaatcca gaaatcactc ctggaggacg cgcattgaaa ttctacgcaa
     4441 cgatccgttt ggaagtacgt cgtgcagaac aattgaaaca aggtacagat attgttggta
     4501 accgtacaaa gatcaaagtc gtaaaaaaca aagtagcacc tcctttcaaa atcgctgaag
     4561 tagatgtcat gtatggttta gggatctcac aagaaggaga acttctagac atggcagtag
     4621 aaaaagacat tgttgataaa agcggtgctt ggtattctta taaagaagat cggattggtc
     4681 aaggacggga aaacgctaaa atctatatgg ctaatcatcc tgaaatgatg gcagaagttt
     4741 ctgctctagt tagagcagct tatggtattg gtgaagaagt cgctgtccca gaagatgaaa
     4801 aaggacaaga agaattgcct ttagtagaag aataaaaatt atgataaaca aaaacggctg
     4861 gtctgaagag taggtactct ccttgacctg ccgttttttt attgaataaa gaaatcgtga
     4921 gttatttaat tatacgagtg ggtacataaa ataaaaatat ctgttcatct acaaagaata
     4981 atttaataga aaagtttctc aatttaaagg tttacctata tgaaactagt gtcatttatt
     5041 ttaaaaacca tcgtttaatg aataagtaga aagccgtttc tttgtttatt taaagttgac
     5101 actacggggt acaaacatta gaataaagtt gtgcatgttt tttgtacaca tatatgcgaa
     5161 gatgcatatt gacgagttta gaataattta tcatagaagc tgtatagatg ataaaaatga
     5221 aaaagatagt atggaggtgg attttatggt attcaacatt ctcctcgcta tcatcggttt
     5281 aattgtcggt cttggtttag ggtttatggt cgcaaagtca cgtcacgaga aagaaatagc
     5341 tggtgcacaa tcatcagctg ctggaatcat cgatagtgca aaaaaagaag cagaaactct
     5401 aaaaaaagaa gcattgttag aagctaagga agaaaatcag aaataccgtt ctgaagttga
     5461 aagtgagctg agagaaagta agttagaatt aaaatctcaa gagaatcgtt tgattcaacg
     5521 cgaacaaacg ctgaatcgta aagatgactc gttagaaaaa cgcgagaact cacttgaaag
     5581 taaagaagaa gcacttagtt caaaacagca attaattgaa gagcgagaaa aagacgttga
     5641 aaaaatgatt gaagatcaac aaaaagaact agaaaaaatt gctggtttat caaaagagga
     5701 cgcacgagat gtcatcatga aatcgactga agaagagctg aatcatgagt taactctaat
     5761 ggtaaaagaa tctgaacagc gtgccaaaga agaagcagat cgcaaagcga aaaacttatt
     5821 atcattagcg attcaacgct gcgcagcaga tcaagtttca gaaacgactg tttcagtcgt
     5881 tacccttccg aatgatgaaa tgaaaggtcg tatcattgga cgagaaggtc gtaatattcg
     5941 aacgcttgag acgctgactg gtatcgattt aatcatcgat gatacaccgg aagcagttgt
     6001 tctttccgga ttcgatccaa tccgaagaga aatcgcacgg atgaccttgg aaaaactgat
     6061 acaagatgga cgaatccatc cagcgcgtat cgaagaaatg gtcgagaaat ctcgtaaaga
     6121 aatggatgaa cgtattcgcg aatacgggga agaggcagcg tttgaagtcg gtgctcatac
     6181 acttcatcca gatctaatca agatcatcgg acgtctgcga ttcagaacta gttatggaca
     6241 aaacgtcttg aagcattcgg tcgaggttgc aaaattagct ggtatcttag cagcagagct
     6301 tggcgaagat atccaattag caaaacgtgc cgggttgctc catgatattg ggaaagcact
     6361 tgaccatgag attgaaggat ctcacgttga gatcggtgcg gaattagctg ccaagtacaa
     6421 agaaaaccca gtagtaatca acgcgatcgc ttctcatcat ggagatgtgg aagctacttc
     6481 tgttatctct gtccttgtag ctgcagcaga tgcgctctca gcagcaagac ctggtgcgcg
     6541 aagtgaatca ttagaaaact acatccgtcg tttaagaagt ctggaaaata tttcgaacag
     6601 cttcgccggt gtagaatcaa gttacgcagt tcaagctgga cgcgaagttc gggtaatggt
     6661 caagccagaa gaaatctcgg atttagacgc tgttcgttta gtacgtgata ttcgtaaacg
     6721 aatcgaagaa gaactggatt atcctggaca catcaaagta atcgttattc gggaaactcg
     6781 tgcaactgat tatgctaaat aataaaaaat acaaaagaag agccgcggca aaagccacag
     6841 ctcttctttt tttgtaaggc acgaaaaaac attgctatca ttatttttta ttcaacatct
     6901 caagatcttt ctcgatcgtc gtattgattc ctttgatgat tgctgggatg aatcctgctt
     6961 cttctaaggc aactacacct gcaacagttg taccgccagg agaagaaact tggtctacaa
     7021 gttcccatgg atttttatca cttgaaagga atttttgaca agaacccata attgcttttg
     7081 cagcaatctc agtggctgtc tgtttggaca aaccattcag tacaccagtc cgtcccatcg
     7141 catcgatcag catgtaaacg aatgcgggtg agctgccagc aaggccaatg aatgtactga
     7201 aatctgattc tgctaaagga tagacgctgc cgattgcttc aaaaatcatc gtagcttgct
     7261 gaagctgttc ctccgtggca gcttcatttt tgcaaatcgc agaaacgcct gctcctacgc
     7321 tgacattgac attcggcatc acacgtacga tggaggcctg aggatcttca agcgctgttt
     7381 ctaaatctgt taatgatttt cctgaagcaa tagaaatgaa taagactttc tggctatatt
     7441 ctcttagttc ttccaatact ttttcaatga tttttggttt cacggctaaa agaacgatat
     7501 ctgattgtga tgcaacttcc tgattacttt tgcatgcatg gacgccagtt tcagcagcaa
     7561 atttttgtgc attttcaaaa tgtccactgg aaacgaacac ctcttctttc gagactgttc
     7621 ctttttttaa taaaccatca ataatagctt gtgccatatt ccctgtgccg ataaatccta
     7681 ctttcatgtt atcctctcct ttagcattag gatatctttc tttttctatc atcgcaagca
     7741 aagaacaggt ctaaagtgga aaaattaaaa taaataagat aacattgggg tatgacacaa
     7801 aagtatacaa gttaggaatg ggaaaatgtg aaaaataaac gatacttgat gctgcaatgg
     7861 ataaaggaac atagtctgat tttaaaactg atttttttcg gctccgtgtt agtctttgtg
     7921 gcaaatcaag tcgcaaatat tgctaatggc atgagttggc aagatatttg gcaaaggatg
     7981 ggacaacaaa gccgcatgac gatcctgctc atgattctgg ctggactgct gggtgtcact
     8041 ccgatgttgt tgtatgattg ggtgacgatc agagtcttag aaaaacaagg aaaaccaaaa
     8101 atggaaagaa aagagtttct tcttgctgcg tgggtaacaa acacgatcaa caatctcgcc
     8161 ggttttggtg gtgtggtagg agcaagtctg cgggctagtt tttatggaaa gcagacaaat
     8221 agaaaaatgg ttttagccac tgtatcaaaa gtcgcactat ttatgctgac tggtttgtct
     8281 gtatggtcgt ttcttacatt tattgatgtc ttttttattc agtcagaaag tatttttcga
     8341 agctattgga tctggctttt cggcggtagt tttttagcac cagtcatttt cttttttgct
     8401 tatctaaaga gaaagacatt gttcttcgag ttttatccaa aaggcttgct tggtctgcta
     8461 ctggcatcct ttggacaatg gacgggagcc cttgcggtat ttttgatcat cggcaagttg
     8521 atgagaatag atatttcgat gctttcgatc tatcctatgt tcatgattgc tacattgata
     8581 ggaatgctga cgatggtccc aggtggaatg gggacttttg acgttttgat gatcttagga
     8641 atgagtcaat tgtctgttcg gcaagatgta gcagtagttt ggctattata ttatcggttg
     8701 ttttattatg ttctaccttt tatgacggga cttctattgt tcatcacaca tacaggagtc
     8761 aaaatcaatc gatttttaga taacattccg cgactgtttt tccaaaaggt tgcgcatatc
     8821 gtgctcgtaa gtgctgtcta ttttgcgggg atcatgatgg tacttctatc aacaatcact
     8881 aatctgtctg caatcagtcg gctatttcaa gtattattgc cgttctcatt caatttttta
     8941 gatcagacag tgaatctatt ggttggcttt ttattactcg gccttgctag agcactttca
     9001 gtaaaagtca aaaaggcatt tctgcctacg attgctttac tatcttttgg tattttaaat
     9061 acaatcacac gtacgttatc ttggcagttg atattggtat atgtcttgat tctgttagct
     9121 gtgtggttat ccagaaaaga attttatcga acaaaattcg tttattcttg gggagctata
     9181 atctttgatg ctagcttgtt cggctttttg tttatcgttt acgcaattgc aggctatcac
     9241 agcggacgct ttttagtcgg tcgaataaca ggaaatcatt ttattttgtt tccttcagat
     9301 gatgtgtggt tctcagggct tattggtttg ggcatctcat tgctagcttt agtggcgctt
     9361 tatcaatatt tagctgaaga tactacgaag cttggcagtg agtacgaaaa agaccgtttt
     9421 gaccgtttat tggaaacata cggaggcaca gctacaagcc atcagcttcg cttgtcaggt
     9481 tacagttatt tttattacca agaagacgac caagatcaag tcgtttttgg ttatcagatc
     9541 aaaggaaaca aatgctttgt tttaggagat ccagttggca atcctaaaat gatgcgggca
     9601 gcaacgaatc agtttatgaa acaagcagat tgtttaggct atcagcttgc gttttataag
     9661 atatcagaaa gctacgtagt gatgcttcat gaattgggtt ttcacttcac gaaagtaggt
     9721 gaagcaggga ttgttgatct agcaacagaa gacttgtctc ggctgagtca gcaacttgaa
     9781 tatcaacgtt tgatgaaaga aggataccat tttacttggt acgatcaact gccggaagaa
     9841 ttacatcaag ctgtaaatga agtttcacaa aaatggcttc aaggtgcacg agaaaaatac
     9901 ttctctgtgg gacgatttga taaatcatat gtattatcta gcggtgtcgg tattgtccgt
     9961 aaagatcaaa aagtcgttgg cttcatcacc gctcagccga tgacgaagaa gcaggcggga
    10021 tatgatttgt tgcgggtctt acctaaagaa tctgaggaat tagctgacta tctgttagcc
    10081 aatctgttta ttgtatacga gaaaatgggc tactcggaag ccaatctagg cttagctcca
    10141 ttagctaatg ttggagaaac agatttttct ttctttcaag aaaaagtcat gcatatcgta
    10201 tataattatg gtaatttttt ctattccttc cagtctgctt atgaaaagaa attaaaatat
    10261 gtcgatcatt gggaaggacg ttattttgct tataggaaag gaagtaattt cggatttgct
    10321 accatgcagc tactcagtct gataggaaaa gaaaaaggaa atgttccgag tttggcagaa
    10381 gaagtgctca cagaattata agaatcacta gaacttgttt ggattatgaa gagaaaattc
    10441 tgtgaaagct gttgaaacga tgacaaaatc aaccactagg cagacataag ctatactttt
    10501 atttttccct atgatagaat acttgcgact tagacttttc gaatgggtga gaggatggaa
    10561 ttgattgaaa aagcgccagc caagataaat ttaggactgg atgtattata tagacggcaa
    10621 gatgggtatc atgagttgga aatggtgatg tcgagcgtag acttggcaga tcatctattt
    10681 tttgaagaaa taacagaaga caaaatcatt gtagaaacaa ataaagcatt tttaccggta
    10741 gatgaacgga acaatgttta tcaagctgct ttattgctaa aaaataaata caatatcaaa
    10801 accggtgtca agatctttgt taagaagaat attccagtag cagctggatt aggcggagga
    10861 agtacagact gtgcagctgc tctaagaggg atcaaccaat tatggaatct gggctgttcg
    10921 ttagaagaac tggcagaaat cggtttggaa gtcggtacag atgttcctta ctgtgtctac
    10981 ggaagtacgg catttgtcgg tggcaaaggg gaagaaatcc aagtattacc ttctatgcca
    11041 cagtgctggg ttgtgttggt caaaccaaga atgagtgtct ctacacgtac agtgtttaga
    11101 gaagtagata cgcaagccat ccaacatcaa gacatccacg gacttgctga agccatccga
    11161 gagcaagatt atcagcagat gatcaaaaaa atgggaaaca gtttagaaga tataacggga
    11221 aagcggcatc cagtcatcca tcaaatcaaa gagcgtatga tcaagtacgg agcagatgct
    11281 gcattgatga gtggaagcgg accaaccgtt tttgccttgt gtcaacatta ttcaagagcg
    11341 cagagagtat ataatgggtt aaaaggtttt tgtgaggaag tttatttagt acgaaccctg
    11401 caataaaata taagaagtaa aataggtgat gaaaaactga ggaatcctaa aatggtattc
    11461 ttcagttttt tattgtagtg aaatggctag atatgagcag caactcatcc attttacgat
    11521 ttaatcaaga atatacatgc ttaaatcaga aattgatgga tttctattga ctgtgttttt
    11581 ttattttgat acattagttc acgttaataa atcgtaaaat tccgatttgt tcattgaaag
    11641 gagcatataa atgaaaaaat atcttttgac agctacggta atgataggtg ctttactgtt
    11701 tgcagcatgt ggaaatacga acaaggaagc agacaaaaaa gaggatttga cgatcgttac
    11761 gactttttat ccgatgtatg attttacaaa agagatcgta ggggatgaag gaaacgtgaa
    11821 acttttgatc cctgctggaa cggagccgca tgatttcgaa ccaagtgcaa aagaaagagc
    11881 agaaatttcc gatgcagatg ttttcgtgta taatagttca gacatggaat tttttgtgga
    11941 ttcgttaaag gattcagtgg atagcaaaca gaccttgatg atcgaagcag ctaaaggaat
    12001 cgaccgttta gaatcgcaag aagcagacga acatgaagaa tcagaagaag ggcatggtca
    12061 ttcacatgaa tacgatccac atgtttggct cgatccggta ttagctataa aagaagtcag
    12121 aacgattgct gaagaattag gcgaaaaata tccagataaa aaggaaactt ttacgaagaa
    12181 tgccgatgct tatatcaaaa agcttgaagc actggatcaa aaatactccg atgagctaaa
    12241 agatgcaaca aaccgcacat ttgtcactca gcatgctgct tttgcttatt tagcgaatca
    12301 atacgactta aaacaagtag cgatttcggg cgtttcacca gatcaggaac ccacaccaag
    12361 tcgcttagct gagctaaaag aatttgtgaa gaaaaacaac atcaaagtga tttattttga
    12421 agaaaatgcc tcttcgaagg tagctgagac attgtcgaat gaaacaggag tgaagctaga
    12481 agtgttgaat cctttagaaa gcttgactaa tgaacaaatc aaagcaggcg aaaattatat
    12541 ttctgtcatg gaaaaaaatc tgaaagcttt aaaagaaagt atcaattaag ttttagaatg
    12601 gaggttgtaa ggtgcgttat atcgaaatat ctgatttgac cttttactat gatgatgaac
    12661 ctgtactaga agatgtctct tatcatgtag atgcgggtga atttgtcatt ttaactggag
    12721 aaaatggagc agccaagtca accttggtga aagcatcgtt aggattactg aaaccttcaa
    12781 aaggaatgat aaagattgct gagaaaaatc aagaaggaaa aaaattaagt atcggttata
    12841 ttcctcaaca agttgcatct ttcaatgcag gttttcctag tacggtgatc gaattagtcc
    12901 gttcaggaag atatccaaga ggccgctggt tcaagcgtct ctctgaaaaa gatcatcaac
    12961 atgtacagcg tgctttagaa gctgtaggga tgtgggatat gcgaaatcgg cgaataggtg
    13021 aattgtctgg cggacaaaag cagcgaatca gtttagcgcg tatttttgct actgaccctg
    13081 acttatttgt tcttgatgaa ccaacaacag gtatggatga agaatccaga aatgaatttt
    13141 atcgtcttct ccgacatagt gcgcatgtcc atgggaaatc agttctaatg atcacccatg
    13201 atcatgaaga tatcaaacag tatgcggaca gacagatccg actggtgaga aaggaggatt
    13261 ctcaatggcg ttgctttcat atgaattcat gagaagagcg tttcttgccg ctatcttcat
    13321 tgccggaatc gccccgatgc taggtgtgtt tttagtgatc agaagacaat cgctgatggc
    13381 tgatacgctt tctcatgtct ctttagcagg ggtagcgtta ggattctttt tgaaccttaa
    13441 tcctacactt acgaccatgc tagtcgttgt attagcagct gtgatattgg agtatctgcg
    13501 aacgatctac cgaacctatt cagaaatctc cattgccatt ttaatggctg gtggtttggc
    13561 gctagcgtta gtattgatga atttgagcgg agggaattca gcaacaagta tccaatccta
    13621 tctgtttggt tcgatcgtga ccattacatg ggagcaagtc atctttttag gaatcttatt
    13681 cgttgccatc ggtatattgt tctttttatt caaacggccg atgtatgttc tgacttttga
    13741 tgaagatacc gcccacgtgg atgggctacc cgttcatttg atgtcgatga tgttcaatgt
    13801 gattactggt gtggctattg ctgtgatgat cccgattgcc ggtgcacttt tgatatccgc
    13861 aatcatggtt cttcccgcag caatcggtat gcgattaggc aaaagcttca atgtcgtgat
    13921 tgctatcagt gtttgtgtag gatttctagg gatgctgtca ggactaacta gctcgtttta
    13981 tctagataca cctccaggag caacaatcac attagtcttt attgctttgt tcctgattgt
    14041 aaatatcatc aaacgaatat tggttgcctt cagaagaaaa aataactaaa ttataaagaa
    14101 aagaagcctt ataaaaacga aaaatttttt ataggcttct ttttatttta ataaataccg
    14161 aactattttc ttattttttc ataaaatgct cgaatttttg aagaaaaact tatataatgt
    14221 aaaacgataa aatttcttaa aaggagttgg aataggtgaa agtacgtcgc agtgaacgtc
    14281 tagttgacat gacacagtat ttattgaatc acccgcatga attgatttcc ttaacaagct
    14341 ttgccgaacg ttatgaatct gctaaatctt ctatcagtga agatttggga atcatcaaga
    14401 aaacatttaa agaacgaggg tatggaacct tggaaacaat cccgggtgct gcaggtggtg
    14461 tattgttcat ccctgaaatt tcatatgaag aggcgaaaaa atatattgaa tctctagcag
    14521 aacgtctatc agaacaagac cgtctgcttc caggaggata tgtttatctc tctgatttgt
    14581 taggagaacc tgaactgctg cgtcaagttg gaagaatcat tgcttctaaa taccttggtg
    14641 aaaaaatcga tgctgtaatg actgtagcca caaaaggcgt acctattgcc caagctgtgt
    14701 cctattatct gaatgcgcca ttcgttattg tacgccggga ctcaaaaata acagaaggat
    14761 caacagtaag tgtcaactat gtgtctggat cttctgaaag aattgaaaag atggaactgt
    14821 ctaagagaag tttgaaacgt ggttcacgtg tccttgtagt ggatgatttc atgaaaggcg
    14881 gcggaacagt caacggaatg aagagcttga ttgaagaatt cgaatcagaa cttgttggca
    14941 tcactgtatt tgccgaatca aaattcaacg gtcatcgcgc catcgatgat tatacgtcac
    15001 tgctttatgt caaagatgta gatactcaaa cgaaaaatat tacggttgtg ccaggaaatt
    15061 atttttcaga ataagaggtt gtgaaaaagc cgcttagttt cgagtagtaa gaaaaatttt
    15121 gtgaaaatgg ctttccacat tttttcataa atttggctta ataccgagaa actggctttt
    15181 gaacaccgtt tatctagagg ttgtgaaatc agcgtcctgt cctgagtatt agctcaataa
    15241 acggaaaaat tgctcctcat attttgccgt ttattgagct aataccgaag gtcagctgat
    15301 tgaaaaacgt ttaatgaaga ggttgtaagc ctaccgttta actctaagtc acaagaaacg
    15361 atttcaaaaa acagcttttc atgttttatg agtagtgtga cttgtgaccg aggagttggc
    15421 aggagagcac cggttaccaa atagataggc ccgttatggt ctatctattt ttttatccag
    15481 aaaacgctgg gataaaagaa aatcacttgt tttgttgaaa aaatgggtat actgggataa
    15541 actgaaatca gcaaactata tttaggagtg acgcagttgg acgcacgtta tgcaattatt
    15601 ttggcggcag ggaaaggtac ccgcatgaag tcaaaattat ataaagtttt acatcccgtt
    15661 tctggtaaac ctatggtaga acatatcatt aatcgggtca gtgaaacgaa accagatgaa
    15721 gtgattacga tcgttggaca cggagcagaa caagttaaag cacaactagg agaacgcagt
    15781 aaatatgcgt tacaagcaga acaattggga acaggacatg ctgttttgca agctgcttct
    15841 ttcttggaag gaaaaaaagg aacaacgtta gttatcagtg gcgatacacc attattgact
    15901 actgaaacgc tgaacaatct ttttgaatat caccaaggaa aaaatgcaag tgcaacgatc
    15961 ttgacggcac aagcagaaaa cccaacagga tatggacgaa tcatccgtga ccatattggg
    16021 atcgtagaaa aaatcgtgga acaaaaagat gcaacaccag aagaagctct tgtacaagaa
    16081 atcaatacag gaacttactg ttttgacaac gaagctttgt tcgatgcgtt aagcaaagtt
    16141 ggaacaaaca atgctcaagg tgaatactat ctaacagata tcatcgaaat cttaaaagaa
    16201 gaaggccata cagttgctgc ttatcaaacg gatgattttg aagaatcaat gggagtcaat
    16261 gatcggatcg cgttagctaa agcaaatgag atcatgagaa agcgaattaa ccagatgcat
    16321 atggtcaatg gcgtaagctt tgtcgattca gcaacgacat acatcgatgc aggcgtagaa
    16381 atcggtccag acacattgat cgaagcaggt gtacaaatcc aaggaaatac agtcattggt
    16441 tctgattgtg tcatcgggtc acattcaaaa atcgtagaca gtaggattga ggatcacgtc
    16501 gtcattgaaa actcagtcat tgaaagcagc catgtcaaga aacatgcaga tgtgggacct
    16561 tatgcacatt tacgaccaaa agctgaaatt ggcgaaaacg tccatatcgg aaactttgtg
    16621 gaagtgaaaa atgctcaaat cggcaaagga actaaagtcg gtcacttgac ttatgttggt
    16681 gatgctacgc tgggagaaga aatcaatgtc ggatgtggtg ttgtcttcgt caactatgat
    16741 ggaaagaaca aacatcatac gactgtcggc gaccacagct ttattggttc tagcacgaat
    16801 atcatcggac ctgtcgaggt agctaaaaac agttcgattg ctgccgggtc aacaattaca
    16861 gacaatattc cagaatatgc gctggcaatt gcgagagcaa gacaagtcaa taaagaagga
    16921 tatgcaaaaa aactccctta tctaaactaa gacttttgca aaatcacttg atttatttaa
    16981 aaaatatgac tactatttat taggtagaat tctagagttg tgaaaaagaa agtggaggtc
    17041 cccatgtcaa aacattactt tgacccaaga ttaaaaattt ttgcgttaaa ttccaatcgc
    17101 ccattagcag agaaaattgc agcagcagtc ggcgtcgagt taggaaaatc atctgttact
    17161 caatttagtg atggagaaat tcaagtaaac attgaagaaa gtattcgtgg atcgcatgtc
    17221 tatgttattc aatcaacgag cagcccagtc aatgataact tgatggagtt gctgatcatg
    17281 attgacgcac tgaaacgagc aagtgcaaaa acaatcaacg tagtcatgcc ttattatggc
    17341 tatgcgcgtc aagaccgtaa agcacgtgca cgggaaccaa tcacagcgaa attagtagca
    17401 aacatgattc aaaaagctgg tgcaacaaga atgctgactt tggaccttca tgcagtccaa
    17461 atccaaggtt tctttgatat tccagtcgat catttgatgg gtgcgccact tattgccaat
    17521 tatttcttag aaaaaggaat caaaggcgat gatgttgtcg ttgtttcacc agaccatggt
    17581 ggtgtcacac gggcacgtaa attagcggaa tttttgaaat ctccgattgc gatcatagac
    17641 aaacgccgtc caaaagcaaa tgttgcagaa gtaatgaata tcattggaca agtcaaaggc
    17701 aaaacttgtg tactgatcga tgatatgatc gatacagcag gtacgatcac attggctgcc
    17761 aatgctttga aagaagctgg tgcaactagt gtgtatgctt catgtacaca tccagtatta
    17821 tccggccctg ctttacaaag aatagaagat tcagctatcg agcgtctagt tgttacagac
    17881 tcgatctatc ttccagaaga tcgtaaaatc gacaaaatcg atgaagtaag tgttggcgga
    17941 ctaatgggag atgcaatcaa acgtatccat gaaaacaaac cagttagtcc attgtttgaa
    18001 acaaaaaata aataataaat aaactcacaa aggggagtcc aattgggctg agattgaatc
    18061 tatttctaaa cccttcgtac ctgtatcggt tatgcgagcg taggaattgt gaataaacag
    18121 ctgtttgact attttcgaaa gctgcttcct cctttgtgac ttttttattc aaaggaggaa
    18181 gtttttttat gggaaaaaat agagtttggg tagaaggaac gattgttgca gcgttagcga
    18241 tggtgttgtc gttgattccg atccagatcg gcagcagttt ttctatttca ttagggcaga
    18301 ttccattgac tctttttgcg ttaagaagag ggacaaaggc agggatgctg gcaggtttca
    18361 tttggggcat cctacatttt ccgttgggac aagtttatta tttaagtgtc gttcaagtgt
    18421 tgattgagta cccgattgcc tatacttttg caggcgcagc agggctcatg gcttcaaatg
    18481 tccaaaaagg ctatgtagac gataatacga aacaaattag aaaaagcatc gtgtttggtg
    18541 cattgattgg cgcactttcc agatattttt ggcattttat tgcaggagtg gttttctggg
    18601 gagcgtatgc actatggggg atgaattctt ggctcttttc tttcgtaatg aatggtgcca
    18661 gcgggttgag tacagctatt gtaacgatca tcgttttatt agggatccaa caaacgactc
    18721 cttcgatttt tgtgccagat aaacgacagg agctgtaaat aaaaaagaag caagaaactt
    18781 gagacagaat catgtcctag tttcttgttt cctttttatt ttttgataag aaatagatct
    18841 acttcaatgg cagataagaa agatcggcat cttctaccaa ttgccagtgt cgatcggtga
    18901 cagagtagat taaagtagcg atgatttcct cttctcgata ccgggtgcct tttaatttcc
    18961 aaatataatc ttcaatcaca tagattggga tacgatattt accatcgatc aaaccaactt
    19021 gatcaaaaga aaaaatgatc ccttcctgca tcaaattacg catttctttc aataattcca
    19081 cactgatttt ttcaacaggt gtttcttttt tgaaaaaaga tttttttaca ttgatcttat
    19141 ctgacaagat cgaacttaat ttactagcaa aaatcctata aaattcatct aaatagcgat
    19201 aataaatccg attcagctga tcattaagat cgaagtatac aaaattattc tgtagtttat
    19261 aaaaaaacgg cgaatgaaga tgtgttttca tatgaccaaa atataataat tctgaaattt
    19321 ctaaaggtgt caattctttc agcatcgtta aatcagtaaa gtcgatccac ttcaattcat
    19381 cgaatccacg cctgttacga gaagaggaaa agaaacgctg aatattttct gtaccacgga
    19441 ttactttcag ccctgtatgg gattcatatt ctccagcaga agcagatgga tcaagcaaga
    19501 gcaagttttg aggaaaatgc acgatactgt ttgcaaagtc agtagcagtc agcccttttg
    19561 ttaaaacagc attacttgtg gtatcaatat gtacataaac tgactcagtc atcatgttcc
    19621 tcctcttatt tcctcttaat ctcttttatt ttacaacatt tgcattaaaa aagcttgttt
    19681 cttttatttt aaaattccat tacaaaatca cgttttcatt gaaaacactc tcattttcaa
    19741 ttaatttttg tgatttttgt attttttatt taaaggatgt tttatgaatt gttttcttat
    19801 ggaatctaca aagtcgaata cgagactcat gaccttttta tgacgtactg gtatacttga
    19861 agaaaaagga gagcgcgatg gagataaaaa aagtttactt ttcacagaaa atgtcgctgt
    19921 attcgcctat ccagcaagca aaacaacccc ttccattttt tcaaagtcat caagaaagta
    19981 gagacgcttt ttggcgttct gcaaaaaagc aatgtgcttt taaagcagta tcgaaaaaat
    20041 ataccgtcta ttttactatt gctaaaagta gaggagatgt tatttttaat aatctgttga
    20101 ttgaaggtga aacttttgaa tggaaaaaat tttttcggta tatggaagct tgcgctcgtt
    20161 tttttattaa agaaaactgt tgttttttat ttcaatctcc tctatcagaa gaatggttgc
    20221 gtatttttca taaaaacggc tatcaaggaa ccacacaatt aaataaaaag ctggtctatc
    20281 atacagcact tgttttaggt ggaggaggag cacacggtgc ttatcagatt ggtgtgtggc
    20341 aggcgctgaa ggaacatgat atcaactttg agattatcac aggtacctcg gtaggtgcgt
    20401 tgaacggcgc attgatttta caaggagata tgaaaaaagc tgtgaattta tggaaaaagc
    20461 tgtcgacaag acaagtgctt gcgttaccag aaatggctgc agttgaagac cttcgtgaac
    20521 gcttctatcg agaaagacgc caaatgacga aaacagcact aattgaaggc ggggtttctt
    20581 ctgctccatt ggaaaagatg gtcaaagaca atttggattt gtccttactc aaacataatc
    20641 ccaaaatacg tttatatacg gtgtctacta aactgccaga actagcggaa gtagtgactg
    20701 atatccaaca gacaaaaaca gaagaaatac cagattggat actcgcctct gcttcatttt
    20761 atcccgctat ggcttatcga aagatcggaa aaaataaata tgcagacggt gggtatcgca
    20821 acaaaatacc tattgatatt gcgattaaca aaggagcaac agaggcattt gtcgtagatg
    20881 tgcaagggcc aggcccagcg aaaaaaattc gtatgccaga catttttatc cattggaaat
    20941 gtcaaacatt gtggacacta ggcagctttc tgctttttga tagccaaagg aaccaattaa
    21001 atttacaatt aggctatctg gaaatgaaaa aacgattagg ctattactat gggaactggt
    21061 atacatttca ttctgttaaa cctgcaagaa catactggcg aggatttttg tcttacttaa
    21121 caaaagaaat tcagttagaa ttatcttttt tcaaaagcat aaaattttgg caaaaactgc
    21181 gtaatttata taagaacagg gtcgtaccag agacatgcgg attggcgatg ctagaacttt
    21241 tagcaaaaaa acatttttta ctgcccaatg aaatctatca agtagacatg atgattcaaa
    21301 tgatctgcca acaagatata aaaagtatgc cggatgattt attggcacaa attggtcaat
    21361 taagtactga agaatggcga aggtatcgaa aataccagca aaaaatacaa aatgagcgca
    21421 cgaaaactgt ttattttgat tatctgctac aaggaaaacg caaggatcgt ttgcagcatg
    21481 aattgcttaa gcaaccagtt gacacattac tgatcctata tttatattat ctcaaggagg
    21541 aacaaccatg gcacaagaat tttcatatga aatcgtagaa gaaatcgcta tattgtcaga
    21601 aaacaacaag gggtggcgta aagaactgaa cctagtcagc tggaatggtc gcccgccaaa
    21661 attcgatctt cgagattggg cgccagacca tgaaaaaatg gggaaaggcc tgacattgac
    21721 gaacgaagag tttgaacaac tgcaaaaagc aatagaaaat atgtaatgag gaaggaccct
    21781 gtcgaatgaa aagtatgact ggttttggca aagccatccg cgaaacaaaa gcgtatcagt
    21841 tagatattga aataaaaagt gtcaaccagc gttttctaga tgttcaaata cgaagtccta
    21901 aaatcctgaa ctttgttgaa aacgattttc gtcagcttat taaacagcag ttaagccgcg
    21961 gtcgaatcga agtatttgtg aatctagctt atattggcaa aaacgaaaaa cagatcgtca
    22021 tcaactggga attgattgat tcacttatca ctcaattgac agacggaatt caacaacgat
    22081 acggtagtca aaatcaagtg tcaattagtc ggatggcgga acgcttcgcg ctggacgaaa
    22141 acttcgtaac ggtagaagaa aaaccggaag agagcatgga agaattggca cagttagctc
    22201 tagatacatt gaaaagcgca cttgattcta ttgaggcgag tcgagaaaaa gaaggtcaag
    22261 ctttagcttt agtgttgaaa aagaatactg aagaattcaa agaagtacta aaaaaactcg
    22321 atagttttgt agaaatctat gaaaaggaat ttcaaataag gtatcaaaaa aagctggagg
    22381 aatttcttgg agcgaaagtc gatgagcaac ggttgttgac ggagctagcc atcttgttgg
    22441 aacgcggaga tattcacgag gaactggatc gtctggcgat ccatgtacaa aaactagatg
    22501 aacttatcga agcacaatgt cctgtaggtc gggaattaga tttcctgatc caagaaatga
    22561 atcgggaaat caacacgatc ggttccaaat caaatgcaat cgaaattaaa aatcaagtcg
    22621 tccagtctaa gacgatctta gaaaaaatca gagaacaagt acaaaatatt gaatgacgaa
    22681 agaggtagag ataatgaaat attatgacgt aacgttccac gaattaagtg gaaaaactgt
    22741 ggttaaacgg gatatccctt ctgaaaaaaa cggctttgac gtatggaaag atgcctgtgc
    22801 agactataat gagaacgaac tgtttatttt gatcaacgac ggtgcttacg tgacgatgaa
    22861 tcggaaattt attgttcgaa ttgacacaga agaggtagaa gatccgacag aaaaagcacg
    22921 ttctcgaaaa gatgagatca tgggtgtagt gaatactttg tccaatatgg gcttttagca
    22981 aggatgctga acaaagaaat acaataaata atcagaattg gatttcttcg cttagcttct
    23041 ttgctctagg aatacattta aaggttgtga caaaattcgt gtcacaatct ttttttattt
    23101 tgaataagtg atgttcaaaa accattttct cggtattaag ccaaatttat gcaaaaatgt
    23161 gagaaactat tttcacaaaa tttttcttca tactcgaaac taatcggctt cttcacgacc
    23221 tcttatacac ggcgttcaaa agcttgcatc tgcgataata accccaacca aacgaaaaac
    23281 atgagaagct gtttatgttt ggctgttctt attacttgat gcaggacagc ttttttacga
    23341 cctcttggta cagggtagct tttttaccaa tttctagcta taattaaaga taaccttgaa
    23401 agttgcagaa aaaaagtgca aaataaacga taagtacaaa aagactagtt gaaaggacgt
    23461 cgtcatgtca gagcgtggat tattaatcgt gttgtctggt ccatccggtg taggaaaagg
    23521 cacagtacga aaagcaattt ttgagagtga ggataatgat tttcagtatt cgatctcaat
    23581 gactacccgt aaaatgcgtg aaggggaggt cgaaggcgtt gattatttct tccgaagcaa
    23641 agaagagttt gaatcaatga tcgaagctgg agagatgctg gaatacgcag aatatgtcgg
    23701 gaattattat ggaactcctt tgtcatatgt caacaagaca ttggatgaag gaaaagatgt
    23761 tttcttggag attgaagtcc aaggagcaaa acaagtcaaa gaaaaagtgc ccgatggtgt
    23821 attcatcttt ttgactccgc cagatttagc ggaattaaaa tcacggatcg taggccgagg
    23881 aacagatgca gatgaagtaa tcgaagaacg aatgaaagtt gcaaaagaag aaatcgaaat
    23941 gatggctctt tacgactatg ctgttgtaaa tgatgaagtg ccgctagcag ttgaacgaat
    24001 caaaaacatt attgccagtg aacattttcg agtagatcgc gtaatcggta aatatattaa
    24061 aatgttaaag gagatgtagt cttatgatgt taaaaccttc aattgactca ctattagatc
    24121 gtgtaaattc aaaatattct ctagtgattt tagcaagtaa acgcgcgcat gaattggatg
    24181 caagtgcaca gcctactcta gattcttttg attcagtaaa aagcgtcggt caagctttag
    24241 aagaaatcga tgcaggaaat gtagtcaatg atccacatcc tgaattaaaa cgtgaacgtt
    24301 tgaagatgga agaagaggaa cgtcaagcac aaaaagagcg tgaacaacac gaattggaat
    24361 cacgtatccg tgaagagcaa aaaatgtaat aaagaaaaat agagcgaaca tctaaaaaga
    24421 aaagcaaacc cagctgtttc cttttcaaac attcgccact tattgtctgg tagaaaaggc
    24481 tgtgtctgaa ctttttgaca cagttctttt ttttcttatc tgctgttaat tttttgttag
    24541 gatttggcag aagataagac aaatccatcg atctctagta aaataggcaa ggaacttttt
    24601 gtcgcttgat aagaaaagac aaaaaaagga ggaaacgaac catgacatta acagctgaag
    24661 tcattgtgga cgtacctact atgcagaccg atcagccatt cacttatttg gttccctctg
    24721 aaatggaaac agctttgcaa gtcgggatgc gagtagaagt cccttttggt aatggcaatc
    24781 gtcatgtcca aggatttgtt atggctatcc aaaaatcaga agaatcagct aatccatctt
    24841 tgaaggcagt tattcgtttg ctagaccttg ctccggtggt caatgaagaa ttactttcat
    24901 tagctgatta tatgaaaaaa attacttacg cattcaaaat cacttgtcta caaacgatgc
    24961 ttccgagtgt gatgaaagca gaatatgata aattgatcta tcctttggca gacacaccag
    25021 aagttcaggt attgtttgat cagcgagaag tgatttcctg gaaggaagca gaagaagaag
    25081 gcagcctttc tcaattgatt cgctggcgcc aagagcaatt agtagatatc aaatatgaag
    25141 tccatacacg aaataaagta aaaacgattc gtttggtccg ttctttgtta actgaaaagc
    25201 aaatcgaaga agaatgggca aaattacggc aaaatgccaa gaaacaaaaa gaacttcttt
    25261 tgtgtttatc tgaactgagc caagaagaac caattgctta tttcaaaaat aaaggaatca
    25321 gtactgctgt cttgaatcaa ggaaaagaaa agggctggct ggagtttata gaatcagaac
    25381 gttatcgcga cccttataaa gaccgagtat ttgaccaaac gacggcattg gaactgaatg
    25441 cagaacaaaa aaatgctgtc gaacaaatca tcacagcagg tcagcagcaa aaagatcagg
    25501 tctttttgct ggagggaatc actggcagcg ggaaaacaga agtatatctt caggcaatcg
    25561 ctgatgtttt gtcagaaaat aaaacggcta ttatgttagt gcctgaaatc gcattgactc
    25621 cgcaaatggt cgagcgattc aaaggacgct tcggagaatc agttgcggtc ttacatagcg
    25681 gactttccca aggtgaaaaa tatgatgaat ggcgcaagat tgagcgggaa gaagcgcaag
    25741 tagtagtagg cgcacgatct gctatttttg ccccgctgaa aaatatcggg ctgatcatta
    25801 tcgatgaaga acatgaatca agttacaaac aagatgagac accgcgttat catgcgagag
    25861 atctagcaat ttggcgcagt aaatatcatc attgcccaat agttctagga agcgcgacgc
    25921 cctctttaga atcaagagca cgagctcaaa aaggcgttta tcagttattg cagttaaacc
    25981 accgagccaa agcagctgca cagcttccag ctattgagat cgtagatatg cgtgaagagt
    26041 ttcagaatca tcggacctct actttttctg ctaatttgca ggaaaaaata caaaatcgat
    26101 tagacaaaaa agaacaaact gtcctactgc tcaatcgtcg aggatattcg tcttttgtga
    26161 tgtgtaggga ctgtggattt gttcttccat gtcctaattg cgatatctct cttacactgc
    26221 atatggatac acgttcaatg cgctgccatt attgcggaca tgaagaacct attcccaatc
    26281 gctgtcctaa ttgtggcggc aataaaatcc gctactatgg aacaggtact caaaaagtag
    26341 aagaggaact ccgagaactt tatccgcaag ctaggatttt gcgtatggat gtagatacca
    26401 ctcgaagaaa aggggcacac gaacagattt tacaaaaatt cggtgcaaaa gaagcagata
    26461 tattattagg gacacaaatg attgctaaag gtttggactt tccagaagtg acattagttg
    26521 gggtgttgaa tgctgatacc tccctgaatt taccggattt tcgttctagt gagcatacat
    26581 tccaattgct tacccaagtt tcaggacgag cagggcgagc ggaaaaagca ggcgaagtcg
    26641 tgatccaaac attcaatcct caacattacg ccatagaatt ggctaaaaaa caaaattatg
    26701 agcagtttta tcagcaggaa atgcatgtac ggcatcgtgg aggctatcct ccttattact
    26761 ttactgtgaa aataaccgcc agccatcctg aagaacaagt agcggcaaag aaaatctttc
    26821 agattgccaa tcagctaaaa gaagtgttaa gccctcaaag cttgctatta gggccaacgc
    26881 ccagcatgat tttgagagtg aaaaaccgat attattatca actaatcatc aaatataaac
    26941 atgaaccgaa tcttcctcat gttttggatg aaattttgaa tggaagccag aaagaacagc
    27001 gacaaggtct ttttgtggca attgacaacg aaccattaaa ctttatttaa gaaagggcca
    27061 aattatgcgc tatcctatat tgatccaccc taatgataaa ttaaaaagaa ctgctcagcc
    27121 aatcgatgta atcacggacg agacaatcgc tttattagac aatttatatg agacaatgat
    27181 tgcgaatgac ggaataggga tcgctgctcc tcaagtagga caaaacaaga gaattgctgt
    27241 gatcgaagta gatgaaggcg agaaatttga actgattaac ccagaaatca tcgaagcaaa
    27301 aggtgaaagc cttgacgtag aaggttgtct cagtattccg catgtctatg gaacggtcaa
    27361 gcgagcagat gaagtgaccg tgcgttatta tgatcgagac ggagaagaga tagaagtgac
    27421 ggcttttggc tatttagccc gtgccttcca gcatgaaatc gatcacttag acggtatctt
    27481 gttcattgag aaaatgatac agcagatccc ggaagaagaa cttgaagaat atatggagga
    27541 acatcttgat gactaagcta gtatttatgg gcacacctgc tttttctgtg ccgattcttg
    27601 aagggctgat agaatcaggt tacgagatcc aggcagtggt aacacagccc gaccgtccag
    27661 ttgggcggaa acgagtaatt acgccgacac cggtaaaaga agcggctctg aagcacggta
    27721 tccgtgtgct gcagccagag aaaatctccg gttcgccaga aatggaagaa atcattgaat
    27781 tggtgcctga tgtgatcgtc actgcggcat ttggccaatt cttgcctgaa aagttattgc
    27841 aagtaccaaa attaggggca atcaatgtcc atgcttcttt acttcctaaa tatcggggag
    27901 gtgcgcctgt tcattatgcc atcatgaatg gggagaaaga aactggtgta acgatcatgg
    27961 aaatgatcaa aaaaatggat gcaggcggaa tctatgcgca agaaagcata ccaatcacta
    28021 aacaagatga tgtagggacg atgtttgaaa agcttagcct tttaggaaga aagctattgc
    28081 tagaaacatt gcccaatatt ttggatggac aaaaaccagt acctcaagac gagtcagaag
    28141 caactttttc gccaaacatc acacgggaac aagaagcaat cgattggcat aaaactgcag
    28201 aagaaattga caaccaagta cgcggaatgc gtccttggcc aattgcattc acgacctatg
    28261 agggcatacg ctggaaatta ttatcggttg aagtattggc agaaactaca gacaaagcac
    28321 ctggtacgat catccggaaa gacaaaaaaa atctatggat tgcttgcggt aaacagaccg
    28381 tcttagccat caatgaatta cagccagctg gtaaaggaaa acaggccgta catgatttcc
    28441 ttaacggaag cggtcagcaa gttcagattg gtcagcaggt gaaataatga aaaaaatccc
    28501 taaaaaaatg aaaaaatcag tacgatatat ggcattactg acattagagc gagtgaacaa
    28561 gggtggcgcc tactcgaatc ttttgttgaa tgagatgatc aaacaaagcg agctaaatca
    28621 aaaagatgtg ggtttattta cagaattgat ctatggtacg attagccatc agcgactatt
    28681 ggaattttac gttgcaccat tgatcgctaa agcaaaaaaa gtggatgact gggtcaagac
    28741 gcttctttat ctttctgtat accaattaga atttttagat aaagtccctg cacatgctat
    28801 tttaaacgag gcggtcgaaa tcgcaaaagt aaaaggaaat cctgggacag ggaaatttgt
    28861 aaatggtgtc ttgaggaatt atcagcgtca aggagcacca gatctgcaag gcattgctga
    28921 tcctatcgaa agactttctg tcaaaatcag cctgcctcta tggatgacta aacgactggt
    28981 ggatcaaatc ggatacgaag aaactgaaaa gctcggttta tcgctatatc agcctagcca
    29041 tgctagtgcc cgagtagata cacgccgact ttccagagat caggccatca ctgttttgca
    29101 agaagaagag atcacagctg aaaaaagcca agtcagtcca tacggtatag tagcgaaaaa
    29161 agggcattta gcttctagct cattgttcca cgatggcgtc atgacgatcc aagatgaaag
    29221 ttcgatgctt gtagcgccgt ctatgcaaat cgagcctgaa catcatgtct tagatgcctg
    29281 tgcagcaccc ggaggtaaga cgacgcatat tgcttctttt ttagatccgt catgcggtgg
    29341 agaagtggtc gctttagatg tacacgaaca caaagtcaaa ctcattgaag ataatgcagc
    29401 acgtctaggt gtctcttctg ctgtctacgc tcaaaagatg gatgctagag aagtcagtaa
    29461 acagttcgaa gcagagcagt ttgatcggat tttagtagac gcgccttgtt caggtcttgg
    29521 gctgcttcgg cgaaaaccag atattcgtta taaaaaacag ccggatgaat tagcgaattt
    29581 accaaaaatc caattggaaa ttttagaaag ctgcgcacca actttaaagt cttccggtat
    29641 tctgacttat agcacttgta cgatcttaaa agaagaaaac caagacgttg tttctgcttt
    29701 cttggaaaga caccctgatt ttgagaggat tgatgttttg accaatccta tgctacaatc
    29761 atcaatcgaa gagaaaatgt tgactttgta tccgcatcag ttttacacag atggattttt
    29821 catttgttgt ttgcgcaaaa agtaatgagg tgaaaaaatg cagattgaat atcaatcaga
    29881 tgtaggaaga cgacgtaaca caaatcaaga ctatgccagt gtatttacca gccgttcagg
    29941 tctcaagctt gctgtattag cagacgggat gggtggtcat cgtgccggag acattgccag
    30001 tcagatggcg gtaacgaatc taggcgaagc ttgggaaaaa caagaagctg ttgacgatga
    30061 aaaaatcgcc caatggttca ttcaatcgat tcaagaagaa aatatgctga tttatcagcg
    30121 tggacaagaa cagccggaat ataacggtat gggaacaaca atcgtagcgg ccgctctgtc
    30181 agatgactgg ttcacgattg ccaatgttgg ggatagcaga gcttatttga ttcgtgaaga
    30241 taaaatcatg caattgacgg aggatcattc tttagtcaat gaactagtca aatcaggaga
    30301 aatcagcgaa gaaatggcag tgaaccatcc aaggaaaaat attttaacac gttctgtcgg
    30361 catgcctgga acagtcgaag tagatgtttc tactcatctt tggcaactaa aagatcgact
    30421 gctgttatgt tcagatggct tgacgaacat gctgtctgaa gaagaaatag gaacgatcat
    30481 caatcaagaa ggcccattgt ctcataaagt aactgagctg attgatcgtg ccaatgaagc
    30541 aggcggagcg gataatatca ccgttcttct tatcgaatat aaggaggaca gtgtatgatc
    30601 gagttgggca aaaaattaaa tggccgttat caaatcactg gaaatatcgg tagcggtggt
    30661 atggcaaatg tatttttggc gcacgattta atcttggatc gtgatgtagc agttaaagtc
    30721 ttacgatttg actttcaaaa tgaccagact gcgattcgac gatttcagag agaagcttta
    30781 gcagcaacag aactcgttca tccaaacatt gttagtgttt acgatgttgg tgaagaagac
    30841 aacatgcagt atctggtgat ggaatacgtc aaaggaatgg acttgaaacg atatatccaa
    30901 acacattatc ctgttcctta tgaaacagca gtaaatatta tgcagcagat cctttctgct
    30961 atttctttag ctcataacca tcaaatcatc caccgagatt tgaaacccca aaacgtactg
    31021 atcgataatg aaggtgttgt aaaaatcacg gatttcggga ttgctatcgc tttatctgaa
    31081 acatcgatca cgcagacgaa tacgatgcta ggatctgttc attatctgtc ccctgagcag
    31141 gccagaggaa gtatggcaac gaagcagtca gatatttatg ctttagggat catcctttat
    31201 gagatgttga cgggtagcgt accgtttgat ggtgaatctg ctgtaacaat tgctttaaaa
    31261 cattttcaag atgatcttcc atctataaaa gcacttgatc caaatgtgcc acaagcactg
    31321 gaaaacgtga ttttacgggc aacggctaaa gaacctgcag accgttataa gtcagccgag
    31381 gaaatgtctg atgatctttc tacagtatta tcaccagctc gtgcaaacga agaaaaatgg
    31441 cagcctcatg tgatggataa tgaaacaaaa gttatcacac cccttacgga agatgcacca
    31501 atgcctgatt cattcaaatc tatgcctctg ccaaaagaca agccagaaaa cgaaacctta
    31561 ccagaggagg aagaagttct gcctgatcct aaaaagaaag gacagaaaaa atggtggctg
    31621 atcctattag ctattcttgc tgttttagga atcggaacag ctgtattctt tgcttctggc
    31681 gggcgaggag aagttgttgt tcctgatgtt tctgatcttt ctgaagcttc tgctagagaa
    31741 acactcgcaa aagcgaaatt agaagtagca acaaaaacag aagaaattgc agatgacaaa
    31801 atcgaagaag gtaatgtagt gaagacagac ccggcagcgg gaacgacagt caagcaaaaa
    31861 cgcgaagtca ctctgtatat tagtagcggc acgaaaaaaa tcaaattaga tgattacagt
    31921 ggtaaaagtt atgaagatgc cgaagcaagt ttgaaagaat tagggttttc tgctgacttg
    31981 attcaaaaag aagaggaatt cagttcagag gtagaaagcg gaatgatcat ttcgcaatct
    32041 ccatcagaag atacagaagt cgatcctaaa acagacacga ttactttcac agtcagcaaa
    32101 ggaccagaaa cagtgacttt gagtagtcat gtaggagaaa actacgaaac tgttcgtaat
    32161 tggcttgtta cacaaggatt caatatcaat aatattagtg ctacttatga ctatagcgac
    32221 actgtaccat caggttcgat catcagtcag gatccagcct cgggtgaagt tgtagcagaa
    32281 gaaacttaca tcaattttgt cgtaagccaa ggaacagagc ctaaattgaa tgatatctcc
    32341 ggctatacaa aaagcgaagc acaaagttat ttagctagtg tcggtgcaga atatatcggg
    32401 catgagactt ccgaatattc aaacacggtg gataaagata aagtgattcg tacagtacca
    32461 ggggcaggaa cgacaattac caaaggaatg gttgtgaacg taatttactc taaaggacca
    32521 gatccagcgt cttctagttc tgaaaaagac tcttcagaca gttctagcag ctcaagtagc
    32581 tccagtagca caagcaactc ttcaaaagaa gaatcttcca gctccacaag ttcatcagct
    32641 agcacctcag aaagttcaaa ataagtgctg aataaaatat atcagaaggg aatgcgacat
    32701 tgatttgttg cattctcttt tttgtgtttc tatctctttt gatcaatttt caagccatat
    32761 actttgagaa gtttgcatgg aataacacct actttcctct tcgtatggta aaataaacaa
    32821 aaacggaggt ggcacttatc aaaggacaaa tcagaaaagc aatcagcggt ttttactatg
    32881 tatacgcaaa cggagagacc tatcaaacaa gaggaagagg aaatttccgg aatcggaaaa
    32941 tcacgccgct tgtcggagat caggtgatct ttgagagtga aaatcaaaca gacggctatc
    33001 ttctggaaat cttgccgaga aaaaatcaat tagttcgccc accagtcaca aatgttgatc
    33061 ttggaatcat tgtcacaagt atcgtagagc ctgcattttc ttataatcta cttgaccgct
    33121 ttctagttac cttggaatac gagtcgatcg aaccgattat ttttttgaca aagaccgact
    33181 tgtcagacga cccagaaaaa atcgaagaga tcaagcaaac ctatactaaa ataggctatc
    33241 cggtgattgt acccaatcat acgggtgata cgacagaact gatccgttac ttcccagaac
    33301 ggctatcggt ctttatgggt cagtctggag caggaaaatc gacgctactg aatcaaatat
    33361 caccggaact gaatttagca acaggagaga tttctgatgc acttggaaga ggacggcata
    33421 caacacgtca tgtggaactg attcctatct atggcgggtt agtagcagat acaccaggat
    33481 ttagctcgat tgactttttg acaattgaag caggagaact gccgagacaa tttcctgaat
    33541 ttgtagaagc ttctgtccat tgcaaattta gagagtgcat gcatgctaaa gaacctggct
    33601 gcgaagtaaa acatagagta gaaacaggtg gtatcgcaca gacacgttac gacaattatt
    33661 tgcaattctt acaagaaatc gaaaaaagaa aaccaatgta tgcaaaaaaa gaaggtaaac
    33721 gaaaatagga ggaaacaaga atgaagatag caccatccat tttgagtgca gattttgcca
    33781 atttacaaag agacattgag ttagtggaaa aaggaggcgc agattacatc cacgtagatg
    33841 taatggatgg ccaatttgtc ccaaatatca cattagggcc aaatattgtt caggcaatcc
    33901 gtcctgttac taaattaccg ttagacgtcc atctgatgat cgttgatcca gaaaactaca
    33961 ttccagcatt tgcaaaagct ggagcagata tcattactgt ccacgtcgaa gcaactcctc
    34021 atattcatcg ggcacttcaa atgacaaaaa gcttaggagt taaagctgga gtagttatca
    34081 atccaggaac accagtctcc atgatcaaac atgttttgcc tttggctgat caagtactag
    34141 tcatgactgt caatcctggg ttcggcggac aaagctttat tgaagaaaca gtagaaaaaa
    34201 ttacggaact cgctgatcta cgtagagaga acaattggac ttatgcaatc gaagtagatg
    34261 gcggaatcgt tccagaaaca gcaaaaatct gtaaagatgc aggggcggat gtcttcgttg
    34321 caggatcata tatctatgga gccgaaaatc cagtcggaca aatcgaacga ttaaaagagg
    34381 cattagacta aatgaaaatt ttattagctg ctggaggtaa tccgcaaaac tggccgcagt
    34441 ctctgctgtc caaatacgat gcctatgtcg ggatagaccg tggtgcgctg tacctgcttc
    34501 aagcaggcta cccagtagat ctggcagtgg gtgattttga ttcattgtcg aacgaagagc
    34561 agaaggaagt atttgcaaaa gccaaagagt atacccgttc ccctgcagaa aaagacgata
    34621 cagatacaca gttaggtatc ttaaaaacat ttgaacgtta cccgcaagcg gaagtcactt
    34681 tgattggagc tacgggcgga agattggatc atcttttagc caatctctgg ttaggtatgg
    34741 aacctcgctt caaacccttt gcccatcagc ttattatcag agattgtcaa aattatttgt
    34801 cttattattt gcctggagag catatagtaa gacagatacc agatatgaag tatttagctt
    34861 attgctgctt gactcccgtc tcgaatctta cattaaaagg cagcaagtat gtattggaaa
    34921 ataaagaagt agaatatccg acatcttatg caagtaacga atttctttca gataccgcag
    34981 aattttcttt ttcagatggt atgattgcag ttattcaaag caaagattga tccataaaca
    35041 aataaaaaaa tttgtctttc aggctaaaaa tgacagaatc tattatttgc taaaaaaaag
    35101 cttaagagga gcaacttacc ttttgtttca cggcatttag tgataaaatt gaacacaaga
    35161 gtctaattaa ttcgaggtgt ctttgtgaag aagttcaatc ctaataaaaa tattattatt
    35221 accttggtta ttgttatcat cgtggtgacg atactaagtg tgaccgctgc gcagcgagcg
    35281 aatgaaggaa agcccaattt cttccaatcc atcgttaatg acagtgttgc aatggttgat
    35341 aaaacaatct ctgccccagt acggtgggta cgaaatggag tagcttctgt ccataatctt
    35401 ttttcaactt atgccgaaaa tgagcggcta aaagaaaaaa tcgatagcta tgatgacgtt
    35461 tcacagcaaa acaagaatta cgagagagaa atcgaagcgt tgaaaaaaga acttgagttg
    35521 aatcagacgc tgactagtta cgaaaaagta acggcaaatg tcattactcg ttcgcctgac
    35581 agttggcagg acatgctgat cgtagacaag ggttctaaag acggtatcga agcaaatatg
    35641 gcagtcatgt cgcaaaaagg attgattggc cgagtgatcg aggtcaatac ggcttcgtct
    35701 aaaatcgaat tactgtcatc ctctaatgaa agctccaatc attttccagt acgggtatct
    35761 tcggctaatg gcgaagcgtt tggtttgctt aaaaactatg atgaaaagct ccatgcctta
    35821 gtggtgaccc aattaactgg tgatacggat atcaaagaag gggatgttgt ccagacatcc
    35881 ggtcttggag ggaattctcc agctaacttg ccgatcggta cggttattaa aacgaaacca
    35941 gatagttatg ggctggatcg ggaagtttat gtgaaacctt atgcagaaat gtatgacgtg
    36001 tcagttgtga cgattgtcca acggctagcg gaggattagt ccatgttgaa gaaagaaaca
    36061 atgaagtact atctgccaat cgttttgttc tttttgatgt tgatagatgg tcatttaaca
    36121 agaatgctag gggagtggtc gaaaggcacc tatatgtcaa atgcccactt tctgatattg
    36181 gcattattat gttgcagtat ggcgtttgaa aaacgttatt tactgattac cacgattgtt
    36241 ctcggggcta tctatgacgc ttactatatt ggcgttatcg gtatctatgc agtagctctc
    36301 cctttaattg tatggttgat gtatgtaatg aaagacgtta tccatgtcaa catctttact
    36361 gaatttttca gtatgatcat ctttgtcacg ggttatgaat tgtttacgat ggtggtccag
    36421 ttgattttta aattagcagt agtaaataac acgtatttta ttacaaggtt tttaggacct
    36481 acactgctgt tgaacatgat tatatttgta ttattcattt ttccctttaa
//
LOCUS       DACEOM010000024        36155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEOM010000024 DACEOM010000000
VERSION     DACEOM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.025"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(18..1484)
                     /locus_tag="IW781_001129"
     CDS             complement(18..1484)
                     /locus_tag="IW781_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1182139.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(1775..3859)
                     /gene="cadA"
                     /locus_tag="IW781_001130"
     CDS             complement(1775..3859)
                     /gene="cadA"
                     /locus_tag="IW781_001130"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1182140.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4001..4180)
                     /locus_tag="IW781_001131"
                     /pseudo
     CDS             complement(4001..4180)
                     /locus_tag="IW781_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(4384..4743)
                     /locus_tag="IW781_001132"
     CDS             complement(4384..4743)
                     /locus_tag="IW781_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1182141.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(4773..5114)
                     /locus_tag="IW781_001133"
     CDS             complement(4773..5114)
                     /locus_tag="IW781_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182142.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5111..5785)
                     /locus_tag="IW781_001134"
     CDS             complement(5111..5785)
                     /locus_tag="IW781_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1182143.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(6961..8259)
                     /gene="asnS"
                     /locus_tag="IW781_001135"
     CDS             complement(6961..8259)
                     /gene="asnS"
                     /locus_tag="IW781_001135"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1182144.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8299..9489)
                     /locus_tag="IW781_001136"
     CDS             complement(8299..9489)
                     /locus_tag="IW781_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1182145.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9510..10037)
                     /locus_tag="IW781_001137"
     CDS             complement(9510..10037)
                     /locus_tag="IW781_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1182146.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10084..12873)
                     /locus_tag="IW781_001138"
     CDS             complement(10084..12873)
                     /locus_tag="IW781_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1182147.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13020..13220)
                     /locus_tag="IW781_001139"
     CDS             complement(13020..13220)
                     /locus_tag="IW781_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1182148.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(13673..13993)
                     /locus_tag="IW781_001140"
     CDS             complement(13673..13993)
                     /locus_tag="IW781_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182149.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14496..15614)
                     /locus_tag="IW781_001141"
     CDS             complement(14496..15614)
                     /locus_tag="IW781_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182150.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16085..17392
                     /locus_tag="IW781_001142"
     CDS             16085..17392
                     /locus_tag="IW781_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1182151.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17513..18712
                     /locus_tag="IW781_001143"
     CDS             17513..18712
                     /locus_tag="IW781_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1182152.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(18739..20007)
                     /locus_tag="IW781_001144"
     CDS             complement(18739..20007)
                     /locus_tag="IW781_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1182153.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(20177..20818)
                     /locus_tag="IW781_001145"
     CDS             complement(20177..20818)
                     /locus_tag="IW781_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ1182154.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(21227..21730)
                     /locus_tag="IW781_001146"
     CDS             complement(21227..21730)
                     /locus_tag="IW781_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1182155.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(21853..22617)
                     /locus_tag="IW781_001147"
     CDS             complement(21853..22617)
                     /locus_tag="IW781_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1182156.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            22725..23000
                     /locus_tag="IW781_001148"
     CDS             22725..23000
                     /locus_tag="IW781_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1182157.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            23560..24510
                     /gene="yidC"
                     /locus_tag="IW781_001149"
     CDS             23560..24510
                     /gene="yidC"
                     /locus_tag="IW781_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1182158.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(24556..24957)
                     /locus_tag="IW781_001150"
     CDS             complement(24556..24957)
                     /locus_tag="IW781_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1182159.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(24971..25780)
                     /locus_tag="IW781_001151"
     CDS             complement(24971..25780)
                     /locus_tag="IW781_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1182160.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(25767..26657)
                     /locus_tag="IW781_001152"
     CDS             complement(25767..26657)
                     /locus_tag="IW781_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1182161.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(26670..27149)
                     /locus_tag="IW781_001153"
                     /pseudo
     CDS             complement(26670..27149)
                     /locus_tag="IW781_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(27325..28266)
                     /locus_tag="IW781_001154"
     CDS             complement(27325..28266)
                     /locus_tag="IW781_001154"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAQ1182162.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(28263..29258)
                     /gene="lacD"
                     /locus_tag="IW781_001155"
     CDS             complement(28263..29258)
                     /gene="lacD"
                     /locus_tag="IW781_001155"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1182163.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(29251..30447)
                     /locus_tag="IW781_001156"
     CDS             complement(29251..30447)
                     /locus_tag="IW781_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1182164.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(30465..31193)
                     /locus_tag="IW781_001157"
     CDS             complement(30465..31193)
                     /locus_tag="IW781_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1182165.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(31634..32608)
                     /gene="lacD"
                     /locus_tag="IW781_001158"
     CDS             complement(31634..32608)
                     /gene="lacD"
                     /locus_tag="IW781_001158"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1182166.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(32605..33063)
                     /locus_tag="IW781_001159"
     CDS             complement(32605..33063)
                     /locus_tag="IW781_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1182167.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(33060..34523)
                     /locus_tag="IW781_001160"
     CDS             complement(33060..34523)
                     /locus_tag="IW781_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1182168.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(34505..35437)
                     /gene="pfkB"
                     /locus_tag="IW781_001161"
     CDS             complement(34505..35437)
                     /gene="pfkB"
                     /locus_tag="IW781_001161"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1182169.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            35579..>36155
                     /locus_tag="IW781_001162"
     CDS             35579..>36155
                     /locus_tag="IW781_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1182170.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT        10587 a         7765 c         6146 g        11657 t
ORIGIN      
        1 taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat ttctttgttt
       61 ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac gattggtgtg
      121 atgatattcg taaataattg gaaccaccat gctttaggat cagcagtata atctactttt
      181 ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc accaaagata
      241 aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt ataagcttta
      301 ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac aactgagttc
      361 aagttcgtca accattttac taattcattc atatctttga taccaaaaat cggcaacaaa
      421 ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt acctttacga
      481 ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa caagattttt
      541 agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt tgtcaatgcg
      601 taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg aaaagcactg
      661 tatgccccat ttgccatcaa gtctttagga atattatcac tagcaaacag catacccatt
      721 gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat catcccttta
      781 ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt ttccgcccct
      841 ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa tgttggaata
      901 tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt gatgaacggt
      961 gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt tccggccagt
     1021 ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa ggcaagaaat
     1081 ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat atttccattt
     1141 ccttggactg cccaaccacc agcaatcaga attgcttgag gcttttgtgc caaataagga
     1201 atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt agatgtcgta
     1261 ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgatcc taattgtcca
     1321 acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga ggtgacaact
     1381 gatactcctt gctgagcata gttattaact acattcccca tgccccaaac catgttaaat
     1441 gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc tctcctttat
     1501 gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt acaagacagt
     1561 gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat tttttccatc
     1621 aaaaaaataa gttattttct tttatttcat tgaccttatt gaatttcaag ctattttgaa
     1681 ttttctgcaa aattaaaaac attagttatt ttataatcct atctattttc tttacatttt
     1741 tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctctttt tttacatgca
     1801 agcttcgcat ggcattcaaa acagctaata ctgtcacccc aacatctgca aatactgcat
     1861 ctcccattga tgcaaaacca aaagcaccta aggcaagtac caaaattttt atcccaatag
     1921 caaagacaat gttttgtttg acgattttca atgttttctt tgctaaatgg attgcttctg
     1981 caatcttcga tggttgatca ttcatgatga ctacatctgc tgcttcaatt gcagcatcgc
     2041 ttcccaatcc acccattgcg attcctacat ctgctcttgc tagcaccggt gcatcgttga
     2101 ttccatctcc gacaaatgcg gtctttttat tgtttctttg taagatttcc tctaaccgct
     2161 gcactttatc ttcaggaagc aattcgctat atatcttgtc cacgcctact tgcttcccga
     2221 tatgatcagc aatttttttc gagtcgcccg tcaacatgac tgtgttcttc acaccagcct
     2281 gttttaattg aaccaatgcg tctacggcat cttctttcaa ggtatctgca ataacaagat
     2341 aaccactgta actttgatcg atcgctagat aaagcaatgt accgatttct tgacttaaag
     2401 gaaaagagat ttcaaatctt tccatcaatt tgtgattacc tgctaatact tgtttgccat
     2461 caacttcagc taagatccct tcacctgcaa attcctggat attagttgtt ggtaaaagcg
     2521 gacctgcgta gccttctaaa acagattgag caatcggatg tgtagaaaac tgttctgcac
     2581 tcgcaatata ttgcatgaat ttctttggat cgatttttgt atcgatcgtc tggatcgaaa
     2641 agtcaccttt cgttaatgtg ccggttttat cgaataccac tgtctcggtt tgcgcaagca
     2701 actccaagta atttgaacct tttactaata caccgatttt acttgcgcct ccgataccgc
     2761 caaagaaact aagtggcacg gaaatcacta atgcacatgg acatgagatc actaaaaatg
     2821 tcaatgctcg atagatccat tcagaaaacg ccgctccaga aacaacaagt ggaggtaaaa
     2881 cagctaataa aacggccaat cctacaacga ctggtgtgta atagcgcgcg aatttagtaa
     2941 tgaaattttc agctggtgct tttttactgc tggcattttc tactaattct aataatttgc
     3001 tgatagtaga attttcataa gatttttcta cttgaactaa aaatggttta tttttattga
     3061 taaatccgct taatactgtt tcaccaacat atacagattt agggactgat tctcctgtaa
     3121 gtgctgacgt atcgactaat gattctcctt cgatgatcgt tccatctaac ggtacacgtt
     3181 caccaggctt cacaagtaca aactgaccga tctgcacgtc ttcagggttc acggtcatca
     3241 atccttccgt cgtctgcacg tgagctgttt ctggacggat cgccatcaat tcgcgaatcg
     3301 attttcttga atgactgact gccaatcctt ggaaatactc gccgatttga taaaatagca
     3361 tcactgctac ggcttctgga tattcaccga tcaacatagc gccaatcgta gcaacagaca
     3421 taagaaaatt ctcatcaaag atttttcctt tgacgatatt catcactgcc ttcttcacta
     3481 catcatatcc tatcagcaga taaatcaata aataccctat caagcgaatt ggttcattaa
     3541 aatcaaataa tagcaataaa gcgatacctg caagggaaaa taaaatccgg aacagcgtat
     3601 ttctctcttt ttcatttcca tgttcagata ctttttcctt atctatttct gttactttga
     3661 catctggttc taattccttc actacttttt ttgtttcttg ttcaatcgta gccaaatgat
     3721 cttcctctgc gtcaatcgtt agttttccag aagtgaagtt taccgttgct tccttgactc
     3781 cattgatctt ctgaactcct ttttcgattt tcattgcaca gttcgcacaa tctaaacctt
     3841 ctaatctgta acttttcatt ttaatgacct cctcgcctca acatatgaac atctcttcat
     3901 aactaattat aaatgaatgg ttattcatat gtcaaggtat ccgaaagaaa aaatgaaaaa
     3961 catctgcatc ggaaaatttc ttccatacag atgttttggc ttattctttc tttttctctt
     4021 cgatcattgc ccctgctctt atccaacccg cacttgcctt cttgatcttg acaggatagc
     4081 aactaagttt gaagcctgtt gctggcaatt tgtctaaatt cattaatttt tcgataggac
     4141 aatatctttt ttcaatccct gcaaaatgtc cttcccagat attcgaagct ccactacgct
     4201 ttcaaaaata tagaaagagt tctttaatga aaattatttc aactttccat aaacagtaat
     4261 cccatataaa taaaagacgt aaaagtcaca tgggagaact tatacaggaa aacacataga
     4321 gtcaggtgaa atacatgtat cccacctgac tctatgcgtt ttctaacctt tattattctc
     4381 tttttataca atttctgcac cgaatggcat caacgccaat cgagcaagtt tgaaagattg
     4441 cttggcaaaa ggtatcccta tgatcgtgat acatagaagg atgccgctga cgacatgtgc
     4501 gagagcaatt ggtaggcctg aaacgatcag ccagatgata ttaacgatca atgacatgcc
     4561 agactggtga taaattactt ctttgccaaa aggcgcaaaa gaaagggagg cgatcttaaa
     4621 acattgcata ccgattggga tacctatgat cgtgatacac caaagtatcc ccgctgctaa
     4681 ccagctaaga ccaccgatta gtccgccgaa tataaaccag atgacatttc ctaaacaact
     4741 cattgtattt ctccttctat tactgttttt gattaaaact gggcaaaaat tgattttatt
     4801 cttctttttt tagcttccag catcaagtat tctgcaaatt ttttttggat ttcttccgta
     4861 ttttcttttg ttactaatgt gtgttggatt agttttcctg atggtcgctg atccacccgt
     4921 tcttctgact gttgcacgtc cgcttgcgca aatccgataa tccgtttttc ttttgttaca
     4981 gcaacgagca atagtttatt cttatcattt aggtcagctg ccgtttttgc ttttacgaat
     5041 gattctggat gatcagtaat cagctgattg atttgaggta aatcttcttt tactgcccac
     5101 cgtatttcca tcatttctca cttttctttc gctcattttt atctccacta aaaaaatcga
     5161 ctgcggcaat tgcatctatc cagctttgtg caatgatctc atgtccgcca atcgttgggt
     5221 ggaccccatc ttctcttgta tatttcgaaa aaccataagc aatccctaaa gcattgaata
     5281 tgccatctaa aggaaccagc aatgcgccat attccttagc aagtttacga acaatttgga
     5341 ttttagggtc taagtcttta cgccagccat gacggtcgtc ggttatagga agtacaaacg
     5401 gctccatcaa gatgatgttg cagatttccg ctgtttgaag ttcatttagt aattttcgat
     5461 aaacggattc aaattgctgc gcagatttct cagttccgaa tatagcctga tcgctagtat
     5521 tatgccaggt atcattgatt ccgatcaaaa tcgatacata gtcaggttgt aaagctaaac
     5581 aatcttcttg ccagcgtact tgcagatctg caattttgtc tcctcctatc ccacggttca
     5641 tgcattcaaa tttcatcgtt ggatattggg agattagttt tccggcaatc aaaagtggat
     5701 aacctttacc tagatcagtt gcttctgctc ggtctcttcc tgcgtctgta atgctgtcac
     5761 cgataaataa tatacgtttt ttcatttgtc aaaaactcct ttttgaataa aacgatgatt
     5821 ttcttactta tattatccca taagccctat caagacgaaa gagaaaatat atgtataaaa
     5881 aatgacacca gcctagtccc ttcaatcaat tgaatgaacg ctcatcaagt gtctagtttt
     5941 ttatactttt tttcttaacg tgccggattc cccttgattc cttcgttgtt tggtccagag
     6001 aaatcgatca cacctaagct tccgtcaatc agaccactga tgacgccatc agaacatata
     6061 tttataggct catctaagac ttccagtaaa tgttctttta ctaatatctc ttctagattg
     6121 cttgggatag attggtcata actttatctg gtccgatgat ttcaaggatg aattaaactt
     6181 acatatggat cattgcatgc tgaaaatacg gacctactcc atcttcgaat gttactatta
     6241 gtttgttttc aggtactaga tgctgcttta tttcaacagc agaagcatca tcaaatcaga
     6301 tttccatttg aataaccttc tttgtatatg tttgattgat tgcgatttcg ttatagaacc
     6361 ttgtgaatca aatagcaatt tcttttgctt tcttctcttg tctgctggaa acttccttta
     6421 tcccgtattt taaaaaaaga actgcgagag ttcgcagttc ttttttttaa ataaacgttg
     6481 ctctccggcc aactcctcgg tcacaagtca caatagtcat caaacatgaa aaactgtttt
     6541 ttgaaattac tccttgtgac tcggagctga acggcaggcg cacaacctct tgatataaac
     6601 ggtgttcaat cagctgaccc tcgatattag ctaaaaaaac ggtaaaatat gagaagctat
     6661 ttttccgttt tgtttctcta atactcaggt caaggcactg atttcacaac ctcttcctta
     6721 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtggaaag
     6781 ccatttccac aaaatttttc ttaatactcg aaactaaaca gctttttcac aacctctcca
     6841 ctaaacggta ttcaaaagct tacatctgcg gtaataagtc ccgccaattc aaaaacatga
     6901 acagctgttt ttgcttggct gttctaatta cttgatgcag aacagctttt tcatcacctc
     6961 ttatggataa attcggttca acaaacgtgg gaatggaata gcttcacgta catggtcgat
     7021 accagcaatc caagtgactg ttcgttcaag gcctagacca aaaccagagt gcggaacaga
     7081 accgtattta cgtagatcaa gataccatga atattctttt tcatccaaac cgtgattacg
     7141 gatttgttct agcaagaaat catagtctgt tgcccgttcg cttccgccaa taatttcacc
     7201 gtaaccttct ggtgcgatca tatctgcaca aattaccacg tcttctcttg ttggatgtgg
     7261 tttcatgtag aatggcttga tggcttttgg atagtttagg ataaagacag gctgttcgaa
     7321 tgaattcgca atgaacgttt catgaggtga accaaagtct tcgccccatt cgatgtcatc
     7381 aaatccattt ttcttcagta attcgattgc atcatcgtaa gtaatacgag ggaatggcaa
     7441 ttttgtataa gtttttaaga cttctttgtc tcgtcctagt acatctaatg catattcgca
     7501 atgatctaaa acattttgta ctaagaatgc tacatattgt tcctgtactt ctaaactttc
     7561 ttcttgatga gtgaaagcca tttctggttc gatcatccag aattcgatca aatgacgtcg
     7621 tgtttttgat ttttcagcac ggaaagtcgg accgaatgaa aagacttttc cgaaagccat
     7681 agcagcagct tccatgtata attggccact ttgagataaa taagcttttt gatcaaagta
     7741 atttgtttcg aatagatccg tagtgccttc tggtgcagaa ccagtcaaga tcggcggatc
     7801 gattttgaca aaatgatgtt gattaaagaa ttcatacgtt gcacgaatga tttcatttct
     7861 gatctgcata atcgcatgtt gtcttgaaga acgcagccat aaatgacgat gatccatcaa
     7921 gaaatctgtt ccgtgttctt ttggtgtgat cggataatcg tggctctcgc ttaccacttc
     7981 cacatttgta attccgattt cataaccaaa ttttgaacga ctgtcttcac ggatttctcc
     8041 agttactaac acggatgttt cttggtgtag atttttagct gtttggaaaa cttcttccga
     8101 cacttcgctt tttacaacta caccttggaa aaaggctgtt ccgtctctta attgtaaaaa
     8161 tgcgattttt ccgcttgatc gcttatttgc gacccaagcg ccgattgtta ctgtttctcc
     8221 cacatggttt ttcgaatcga tgatttgaat ttgttccacg tatgactctc cttttatatt
     8281 ccttgaattt cttcaggatt aattttgact tttactctca ataaattgct tgattctgcg
     8341 taccgcttct tctaacgtag cgcgatcagt ggcataactc atgcggacat tttctggtgc
     8401 cccaaagcct tcgccagtca caagcgctac ccctgtttct tctaaaagat cttctaccca
     8461 ttgtgtgaca ttttcatact tacacatgcg tagcgtttct tttacattag gaaaaagata
     8521 gaaagctcct tgcggctttt caagagaaac acccggcagt tctgccacaa gcggatataa
     8581 cgtattcagg cgttcttcaa aggcgttccg catttcttct actgtatctt gctcaccttt
     8641 caatgcttca acagctgcat attgactgac tgctgtcgga ttgctagttg attgggaagc
     8701 aacagcgatc attccattga tgatcacagg atttcccacc gcataaccaa tgcgccagcc
     8761 tgtcatcgca tatgttttag acacgccgtt gatgatgatg gtctgatttt tgattgcttc
     8821 tgaaatcgtt gcaattggag taaactcatt gccattataa actaaacggc cataaatatc
     8881 atcagcaact atcaaaatat cattttttac tgcccattca ccgattgcct gcagttcatt
     8941 cttgctataa atcattccag tcggattcga tggagaattg atgattacgg cttttgtttt
     9001 ttctgaacgt gcttgttcta gctgttcaac agtaactttc cagctattgg attcttcccc
     9061 tttcacaaag acaggaagtc cttctgccag ttttacttgc tcaccgtaac tgacccagta
     9121 aggcacagga atgatgactt catcttgcgg gtcaaggatc gtttggaaaa gggtatacaa
     9181 agcaaatttc gctccgtctg ttacgactgt ttgtgaaggt tcatagacaa gtccatagtt
     9241 tttcttcaga taatcaacga ttgcctgtcg aagctctggg attcctgctg ttggtgtata
     9301 atagctagct ttcccattgc ggattgcttc gattgctgct tcttggatgt tttctggtgt
     9361 cgcaaaatcc ggttctccca cagtcaaact cagcacatct ctgccttttg cttttaatgc
     9421 cttggccttt gccgcagctg ccaaagtgac agacggttct aatcgttttg ttctttttga
     9481 taactccatc ccattcattc ctttaccttt taaattccct tgattgtctt gacttcatct
     9541 ccagattcaa agccaatcag gtaataattc agtttcccat ttgtttcttt tcctactact
     9601 tcccacacag gctgatcatc atacatccct aaagcagctt tttcgatttg gatttcagga
     9661 tgggccgcag caattgtctg ttttgcttcg ttttcagtca gtccctttgt ttgatccaat
     9721 acttttactt tttcaccaga tttaggaatg ataacggcga tttcttttcc tttatcattt
     9781 gtacctgtca gtgaaaagta tgtcttcttt cgagtaaacc aataaaaatg atcggtttcc
     9841 tcaagatttg catatttctt tgcgatttct actgcttctt tcttcgcctg caccgccgga
     9901 cgactggaac gcaagtaaaa aatcgagctg atcacgatga ccgctaataa aaatacgatc
     9961 aagccaagta acacgcgatt aatcgttttt tcctttgtct gttttttctt caagggtcgt
    10021 cgctccttta tcttcattcc ttttcattat agcagatttt aattatgaaa aaagacagct
    10081 cctctaactt ccttcttcat ttttctttaa gaattcatgg atatcctgat agatttcctc
    10141 catagctagc tctttgatcg gtactttttt cggtaatgca tggatgatcc gtgcgccata
    10201 ttttgcagtg actagtcttc gatccaaaat aagcaatacg ccacgatctg tctccgtcct
    10261 tatcaagcga cctaatgctt ggcgtaactt caaagctgct ttaggtacgg attcctggta
    10321 aaaaggattg atcccctctg attctaaata agcgttccta gctttgacca tcgggcgaag
    10381 cggattatcg aagggtaaac gtgtgactac aacgatctgc aaagcatctc caggcaaatc
    10441 tacaccttcc caaaagctgt cagcgccaaa caatacgctt ttttctgaca aaatgaagcg
    10501 tttcaaaagc ttctcccggc ttccaccaaa cccttgagct agtatttctc gtcctttttc
    10561 tagcatagac agatgcattt tctgatagac cttctgcaaa acatcatgag aagtgaataa
    10621 cacaagtatt ggccgatctt cttgttcgat cagacctgta agtgctgtct ctaaataatc
    10681 gatatatgct tgattgctga cttgatggac ggcaggtgcc tcttttggca catagatcct
    10741 tgcctgtttc tcataatcat aaggagaggc aatcacttta agaggtgctg cttcaatgcc
    10801 aagcctctta gagagatagt gtcgattagg gccgatcttc agcgttcctc caatataaag
    10861 aatacgatca tatcgaggat accaaacagt cgacggcaac atagccgctt gaaaatcatg
    10921 gagatgaaaa atcatctgct gcgctgtgtt cccaggaacg aaccaatgga tcaaatgatc
    10981 tcgccagtct tccagccatt gatccatcag caagtattgc ttgtctaatt gatcaaatag
    11041 gctatataaa cttgctaaat cgatccgcat ctggtttgtc cagctttctt ggataatctc
    11101 aaaagtcaga cgcatttgtg actgtagctg ctggatctcc tgataaaaaa gctgcaggtg
    11161 atcaatggct gtcccagatt ccagtggcag ctgatccaac atctcttttg tcatcatgac
    11221 ttcttctgga tactgtccgt taggttgctt gccgctcact aaactcatta atccttctgc
    11281 catacaaatc tgtgcttcgc atagcgcaga aagttcttct cgataaatcg ataaccagcg
    11341 taaggcttca tgttctttcg gtaaatagct ttgtattcgg tcaaacagct gatttggttc
    11401 ttctaattgc tggatttgtt tatggaaata gttaatactt aatcgtttgt catttacttt
    11461 ttcagcgatt tccggcaaat gatgtacttc atcgatcaac aaaaatggac tagctggaag
    11521 caatggttct tttcgctgtg tttcttgtac taaaaacgca tgattgacaa tcaaaaaatt
    11581 actttgcctg attttctttt gcaagaaacg aacaaagtct tgttcgtaaa atgactgatt
    11641 tcttgctaaa aatccaatac ctctgtgcgc gatatctgca aagaatgaat ggttcaaatt
    11701 tgttaaatgt aattcatcca aatcaccagt cttcgtttgc gtcagccaaa ctaagaccgc
    11761 catttgatac aaagcatatt gtttttgttc tggcggttcc acaagtgtgc ctttaaaacg
    11821 ctgcaaatca atatagtggc gatagctttt gataattaca ggttgtatgg gctgatctag
    11881 caattgattc agcaatggaa tgtctttttc tatgatctgc tgctgcaaaa ctagcgaaac
    11941 ggtactgatg acagcgggtt tttcaggtgt agccaaataa ctcaatggca gcagataacc
    12001 gattgtttta cccattcctg tagctgcttc gatcatcaaa tcttttgatt catcttttgt
    12061 gaaatggtcg tagaccgcat tcatcagtct gttctgttct tttcgataca tcagtgtttt
    12121 accaaacatt ttttcttttg cttgtttttt tcttggatat gtccgctcgc cataatggac
    12181 ggatgtgaat agttccactt cttttttctg caaagctagc ccatcgacga tttctaatga
    12241 gggatcaagt ggctctggtt tttctttcat ttcttcaatc acatgataaa tgaaacgact
    12301 cgtatccata cctgtgtgtc tacttaatcg cgcaatctgc tctaacgtga tgattggcaa
    12361 ttttctcatc cgttcttcga tcagcaaaag aagttgacct gtcacttcgg catcactatc
    12421 tgcctgatga ggattctcat gaataaatcc aaggctttct gacaaatcag ctaagcgaaa
    12481 gcttggttcc gtcggcaaga aaatttgtgc aagttctact gtatcgatgc ctggtatctt
    12541 caaactagga agtccgcatc gcatgagttc atggttcaaa aaattataat caaaataaat
    12601 attatgcgcg acaaatatgg tgtctgccaa aagattgtaa atcgtttggg cgacatcttc
    12661 gaaataaggc gctttttgga cgcgttgatt cgtgatccct gttaaatgct ggatttgttt
    12721 tgaaatcttc cgccctggat gaatatcgat cgaaaaacgt gaagtgatcc gacctgattc
    12781 aatcatgacg cagccaaatt ggatgatccg atcttctttt ggattcgtcc cagtcgtttc
    12841 gatatcaacg actgcataag ttcttggttt caaaatatct tcctcttctc ctgcctctaa
    12901 tgttcttttg aaattatact acaaagtgca gaaaaatact ctcatcgctt tttaaagaaa
    12961 atagacaaaa acgcccagac acgcaaatgt ctgggcgttt attttttagt ataggttaat
    13021 taagctttag taacgtttgt tgcttgaggt ccgcgttggc cttcttcaac atcgaaagtt
    13081 actgcttggc cttcttctaa agttttgaat ccgtcgcctt ggatagctga gaaatgaacg
    13141 aatacatcgt ttccgttttc tgcagtgatg aatccaaatc ctttttctga gttaaaccat
    13201 tttactgtac ctgtttccat aataaaaatc ctccttgtgt atcaacacat attttgcaat
    13261 tacttgaatg gtgtatatct ggaagatgtt tatttgaaac aagctaccac agattaccga
    13321 acaaaaacta ctcctatagt atatcatacc gtttacaaaa aagctagagt ttttcgactt
    13381 tttttattaa aaaaagtttt ctcagcgatt acataaagtg aaaaaccgaa ctttttttta
    13441 aaataaacga catattgttc agttggtgtt tttttgctct gatgttcttt tttaaactaa
    13501 aaaaattatt ctttctgact acttatcggt tctgtttcaa cggtcgaggc tggggaagat
    13561 tctttagtag gtctgatgtt ctcatggtat ttctgatagc ctatgacagt taccagaaga
    13621 acaaggcaaa ccaatacgac caacgagatt agtttttctt ttttgctcat atctatacct
    13681 cataaataac aggatgatgt ccatcatatt tctgagtatg agtaaaagca aaagctgcag
    13741 ctgtagaatg taagaaatgc cagcaacttt cataatcaca ggtcggacag taccaaaagt
    13801 aaccaaatct tggctgcaaa gaccgttctt ctgtttcagg aatcgcagaa tcaagatctg
    13861 gtgtctgtac ataaacattt ttgtacagac gtgtgcctgt aataggaaac cgctgtgttt
    13921 cttcagcaaa aactggctga cttgttaaac ttagggatag agacagcaaa caccataaca
    13981 gtcttttttt cataaagacc tccttatcga taagtttttc ccaattaaaa tcatatctca
    14041 agaacgcccg ttattcacat tttgttcata aatgatgttt tttgtatcat aatcgtcaaa
    14101 aagaggtcgt gaccacgtca caacctcttt tttaaacggt gttccagaag tagcttcttc
    14161 ggaaataagc cgaaatttca caaaaatttg agaaataatt tttgtcaatc tcttcttatt
    14221 tcttgaagct gaccacttct gtcacaacct cttccttaaa cggtgttcaa aagcttgcat
    14281 ctgcggtaat aagcccacca aacaaaaaat atgaagagct atttttgctt gctgttctta
    14341 ttacttgatg cagaacagct ttttcacaac ctctcataca cggtgtttaa tcagctgacc
    14401 ttcggtatta gctcaaaaaa cggcaaaata tgtgaaacta tttttccgtt tgtttctcta
    14461 atactcaggt caaaacactg atttcacaac ctcttttaat aacgaagaat cgctgtcaga
    14521 ctcttttcat ctggcgcatc tttttgatcc aaaataaaca cttttccgcc attaatgatc
    14581 acatcatctg ctaatgcatt caacacttga cgccgatcat attctgtttc aggatcttca
    14641 ccgaaaccat cagctaagtt tgacgtagcg ataaataaat gtgacacttt tccttctttc
    14701 gcagctggaa cgatatcaac taactgatcg atgaatttct tatctaagag tttattgtat
    14761 tcttttgatt cttgagctgc gagttcactt gacagcttct tcgccccttc ttcgatatct
    14821 ctaaaggata gctgcgcagg agaagcagaa atcgcaacgt ctgcacaata atagggattt
    14881 ttagcaattt ttttgaacat cgtctgattt tctggtagag caaataaata aatcggccat
    14941 tgttctggat tttcgaaaga atccttcaag tacgtatcaa ctgcttggta atagttgacc
    15001 caatcgatct ctacttcttc atcttttgga tttacgccat ggaaagttac tccatctcgg
    15061 gctccgcctc ctccattaga agagaagttc aaaccgccac ctgtcagttc agatcccaat
    15121 gcagtaacga catcaaccgg tgccccttct ggtagagata cttcttttac ttgtttgttg
    15181 ctgacttcat acaatttcat ggagtcgcgg ttcaatccca ataaataata agaatagttg
    15241 aactgcccat ttttgataat agccagtaaa taaggaagat cacttacata gtattgatcg
    15301 tctaccggga tactcaaacg atggatatag gtttggtcag cagttataat gaccgctaca
    15361 ctcgtagttg cactacgcca aaacgaagga tctcctaaca aattttcaat tttttcttgg
    15421 aacggggacc agtcatgttc ggaatatttt ttctcaaaac gtttctttgc ttctttcgca
    15481 aaatttttga atttgatctg atctttttcc acatcttgat gggcgacatg cgtattcagc
    15541 atgatcgtga cgaatggtcc tttagcgcct tctgaagtca attgtgacaa taattcttta
    15601 tccttttttg ccataaacgt acccctccta aaaaaatcat tcctttgttt ctatctaaag
    15661 tctaccacaa cactattttt ccatacaata aaacgtatct aaaaaaataa aacggtttct
    15721 acggttttaa atttagctat ttttttaagt tatcttcata atttcctcaa ttatttagcc
    15781 ccagtcttca actattcttc atgaaatgct gataaactaa gaaaaagaat aaaagaggtg
    15841 gatcttatgc atttgattat gattttcagt ttattaagta ttatcagttt atttgctttc
    15901 agcgcttccc gcttattaga tgcggaaaca cagcctattc ctgtaaaagt acgtaaaaac
    15961 gaccgctaac cttccataac gtgcgttctt atcctaaagt tttttcagaa acattgaaac
    16021 tctctcccct ctctgataaa attttaattt gtagcgaaac aaattaatta caaggagatc
    16081 ggatatgtcc ataaaaaaaa cacgtctagt cctattattg gctagtctca ttctttgcct
    16141 tgcttctttt ctaccagctg ttgctgtgca agctgaagat actttcaaag taaatgcaaa
    16201 agcagccttt gctgtagatg cgcaaagcgg taagattctt tatgatcaag atggagaaaa
    16261 aaccatgggg atcgcttcaa tcacaaaaat catcggtctt tacatcgtcc ttgatcaagt
    16321 aaaagaaggg aaactatcat gggatgataa agtttcaatt tcagattatg cagaaaattt
    16381 gagtgtcacc ccagatttat caaatgttcc tttgcataaa gaaaacacct acaccgttaa
    16441 agaattattc gactctgcaa tcatccagtc agccaacgct tcaatggttg ccttggcaga
    16501 aaaaatctct ggcagcgaag caaaattcac tgaacgaatg aaagaacaat tgaaagattg
    16561 gggtatcaaa gatgcaacga tcgttaatgc atcaggactg aataactctt atcttggcga
    16621 aaaccgacca gaaggtactg gagaaaacga cgaaaatcaa atgtctgctc aagatgtggc
    16681 tatcgttgcc cgtcatttga tcttggattt tccagaaata ctggatgtat ctagcactac
    16741 gacacaaatg tttggcgaaa acacgcaatc accagtagaa atggtcaact ggaactggat
    16801 gctcccagga ttcgtgaatt ataaagaagg cgtggacgga ttaaaaactg gtacgactga
    16861 acttgcagga gcctgtttcg tcggtacgat cactaaaaac gatcaacgaa tcatcactgt
    16921 cgtattgaat gcagacggcc atgcggaaaa cccaagtgcc cgtttcaatg aaacagctcg
    16981 cttaatggat tattgctacg acaattggtc agaaaaagag cttggaaaag ccaatgcaag
    17041 tatccctagt ctaaaaacca ttgatgtaaa agacggaaaa gaatcaacag tgcccgttgt
    17101 gcttaaaagt cctgtaaaag tatgggtacg gaacgatatg gatactggaa aattaacaat
    17161 cacaccaact cttgacaaaa aacaagtcac tgacaatgag atccaagcac ctatggataa
    17221 aggaacaaaa atcggtaccg caacgatcac cttggcagaa gataaattag gttatcttga
    17281 agacaacaac gcgccatcca cagaaatcat cacggataaa accgtagaaa aagcaaatat
    17341 tttcgttatt ggctggcgac gcgtcgcaga atttttcggt aacttgtttt aattgaacaa
    17401 aaagaaaaaa gcatctttcc atgtggaaga tgtttttttg ctttttctag tttaaataac
    17461 agcttttttc atctattttc gttatactgt ataagaaaaa ggagttgaat ttatgaatct
    17521 aaccagagat ctgatttttt tggttctcta tcttattggg gtattctcca tcgtttattg
    17581 gcgaaaacag aaaaaggatc cttccttgtt cttttacagc agtacatgga cgtttgcggt
    17641 aatcttgact tattttgtcg tgttggaaat tttttattcg accttttatt ttttgattcc
    17701 cttagtgttt ttcagtatct ttgccttttc ctatttttca gaaaagcgaa ggctgttgaa
    17761 tggtctttta ttcaatattt tcctcattag ttttggtatc tacttgtttg tcttgctata
    17821 cgaaacacaa aatatttttc ttggcggact gattgccttg atcacgatcc cgttactgct
    17881 tgtctttcta ttcggcattt atggattgat cgtttttctt ttttggaacg gtgtcacagt
    17941 tttacgtaga gagtcgcatt cattagctaa tcttttaacg ttgatactag ccatttttct
    18001 tacattgttt cttgtttttg actttttctt gttaaaatat ttaccacaat ggataaatgc
    18061 tttgtttttc tgcatcccat tgatcttgat ctatctattt atcgtattct acaacttttt
    18121 gactgtctca ttcttgtatc agttcaaccg ccctcgatac aaccaagact tcatcgttgt
    18181 gttaggcgcc gggttgatca atggagaaac agtttctcct cttcttgcca agcgaatcaa
    18241 taaggcgatt gctttttatt gggcacaaag ccgtgcgaca ttaaatccgc ctatcctatt
    18301 aatgtcgggt ggacagggag cagacgaaaa agtaccagaa gcaatggcga tgaaacaata
    18361 tgccatggaa caaggaattc ctgaaagaga tattcttgtc gaaaccaatt cgacaaccac
    18421 tttggagaat atgcgctatt caaaggaaat catggatcag caaatgaaag gtccctatcg
    18481 cgcgatcttc agttccaaca attaccacat tttcagagct ggtctctatg cacgacaagc
    18541 gaaactgaag gcaaatggca ttggggcaaa aacagctttc tattacttgc ctaacgcgtt
    18601 tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa agacatatga tcatttgcgg
    18661 attgcttttc gcaggttccg cattcttatc actgatcact ttacttttct aaaaaaacaa
    18721 ggtgtgacaa aacatatgtc acaccttgtt tttttctttt attctcttga cgattggcaa
    18781 gaatagtatt ccttttttga cggctacttg ctgtgcagaa taaataccgc tgtttcctga
    18841 acacataaag ctgatcaagc agatcataaa gaagtaagca gcagcttgtg aaccaaataa
    18901 ttcgattccc atcacaaaac aggcaatcgg tgtatttgtc gcaccagaaa ataccccgat
    18961 aaaacctaat ccagcaagaa aaggaacaga aacatggagc aaaggcgcta gtactgcgcc
    19021 aagcgttgca ccaatctcaa atagtggtgt cacttctccc ccttggtacc ccgcacctaa
    19081 agacaagacc gtgaagaata atttcccaat aaaatcataa gcatgtgcct gtccattaaa
    19141 tgattcctgc agtaacggaa gactcaaccc aaggtaacgt tgtgtttgca aaatcaacgc
    19201 tgcggctaca actactgctc cacctatcgt atttcgccaa acaggatgag gaatccattg
    19261 cgcataccgc gcttttaaaa agacgatcga acggctgaat acccaaccaa tcaaaccaaa
    19321 agcaatccca gcaacaaaaa ctttgaagaa cagcaacata ctccattcag gaatcacacc
    19381 catttggtaa tgtagatgag aaacaccata gctttctgtc atgaaatttg caaaaagcgc
    19441 agcaaagaag ctaggaaata ttgcttctgt tcggacttta ccgattgcca acacttctaa
    19501 tccaaataat gttccagcta aaggtgtgcc aaatacagaa ctaaaacccg cactgattcc
    19561 actgataacc aaaatctccc gttctgcttt atccaatcgg aataaatgcg ctatattatc
    19621 agctaatgct ccccccattt gaactgctgt tccttcacga ccaacagatc ccccaaataa
    19681 atgggtagtg atggtcccaa acaaggtcaa tggaatgagc cgcaaaggga tcttctcttc
    19741 tcctccattc ccttgatcga tcactaagtt attcccacga ctagaaagac cgccatgata
    19801 cgcataaaga taagcaaaaa gcgcgccgct gactggcaat agataaagta gccaaggatg
    19861 tgctagtcgg atatctgtca cgaacgtcag gctatttaag aaaaaagcag acaagctccc
    19921 catgaatact ccgagaatcg ctgtgaccac cagccacttc aacatgtaca tcaaaatacg
    19981 cactggactc ggtaattgtt tttccataaa aaattcctcc tcaaataaaa aaacgcctac
    20041 taaagaaagg ggctggcccc agactttagt aggcgtcatt agctatctta cgttagcatt
    20101 taaaggcgaa caccatcacc tattcacttc cattaaaaga tagcatattt ttctgtaaaa
    20161 aacaataaaa ttatcgttat ttctttacac gttcagcaaa ttcatagaaa ggagccaatt
    20221 tcgttaactg ttgattacat tgatcactat catgacaaat ataattcccc tttttcgtat
    20281 acgtgccatc agaaccggct ttcgttgtag aaagaaagag cgagacattc gatgttttct
    20341 gacagatcgc acagattccc ttattaatcg ttggtgataa agtaccgctg atccctttta
    20401 attttccatc ttcgtaataa accatgaatt ttttttgcgt ccctggatca ttccaaccga
    20461 tgtacgtatg tgcttgcaag tcgatttcat tccaagcggg ttgtttgagt tttttcactt
    20521 ttcggaataa tttctcgatt tgtggtttgc tcggctgcat aaatggctgg acgatttgct
    20581 tcagctcctc caatgctcgt tctgcttgcg tttttgtcag ttttacatct aaaactgtcg
    20641 ttaaaaacgt atcgatttct gatggttcgt aacctaatac atcgcgaatc ttttcagctg
    20701 ctagagcctg aaccgtttgt accgtttttg agtcattgac agatgagtac gcatttaata
    20761 actgctctac ttctcttttt ataaaaaaat attgatatgg tttcattgtt tgttccatga
    20821 ttttaaagtc ctttcttcta accaaagtgc atactttgta gaaaaaagtg atcttctgca
    20881 aagtatgtgt tacctgaaga tcccggactc ttttaaataa gatacgtttt gcatcttatc
    20941 atcaccccct ttaatgttca ttcttctatg tacatgattc attcgataaa taaaagtaaa
    21001 ccgtatcttg agcagatagt caactgcaaa cagaaaaagg actgtgacaa aagtcgtgtc
    21061 aaagtccctt tctacaaaca aacggtgttc cagaagtagc ttcttcaaaa aataagccgg
    21121 aatttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
    21181 ccacttctgt cacaacctca atatggtcta ttattggttt tacctgttaa atcgaggatt
    21241 cttttccgaa gtaacgttgc catctcttac gcatacttgc tgacataggc tgagcggctt
    21301 ctttgactgc gcagtatttg tctacgaatg ttacgatata tccttctttg tacttaggcg
    21361 gtgcaatcgt tgcgccccac atgtgcttca aaatgatatc tcgctcaaga tccgacaaat
    21421 cggtgatctt ttctgcattc tttacagcga ttcttggatg gatatacgca tgtgtgcctt
    21481 catcaaattt cgtcgtacgc caatcatagt agaacagatc atgcaaaagt ccagcacgcg
    21541 ctgttgcttt agcattgcca ccccattttt ttgcgagcag ataactatag taagaaacac
    21601 tgatggaatg ctccaaacga gtggaatgaa catgctgtgt gtaattcgct aattttttca
    21661 ctgcttctgt ttctaaaaga tcttctacat aagacatata ttcttcgtct tcacgccatt
    21721 tttctgtcat aattatcgat ctgccacctt tttgaatttc attttcttct gaaattgatc
    21781 taagtatacc accagtgttc ggattattct agtcgccttg gataaaatag ccaaagctta
    21841 atagttcctt aatcataaag aaaaatgata aagaacaatc cctgccgcaa ccccaacatt
    21901 caacgattcc gcatttcctt tcatcgggat atatacgttc tgatcagtca aagacaagat
    21961 ttcctgactt accccctgac cttcgttccc catgattatt gcgaagttat ctgatttttc
    22021 tactttgtct aaggcgtgtg cttcttcatt taattctgtg ccatacactg taaacccttt
    22081 gtctttcatt aaaggaatca cttctgtcaa atctttttgc aacacaggca aatgataatt
    22141 gcttccctgc atgctgcgca gcacctttgt actataaata tctgccgttc ctttgcctaa
    22201 taaaacacca gccatatcaa atgcatctgc cgtacggatc atcgtcccta catttccagg
    22261 atcttgtaca ttatctaata acagccatcc tcctgaaaaa tctggcgtgc cttcagccgt
    22321 taacgccatc actgctaaga ttccttgtgg tgtaggtaga tccgatacag catccatgac
    22381 ctcatccgaa actagaaaag tagaaatctc tttgtttgcg gccagccagt ctccccattc
    22441 tttcatgcct cgctgtgtca caaaaagttc ttttatttct gcatcagacg acgcagcttc
    22501 ttcaatcaga tggaaccctt ccaaaagata ttcttccttt tcttcccgat atttcttttt
    22561 atgtagtttt tttaattctt tgattcttgg attttttgct gattggatct ctttcacatt
    22621 cctcacctcc ccatgattat atcatggaat aggtgcttga gcttgtttca tcctaaaaaa
    22681 atcggtatca ttaatttata aaataggaaa gaaaggtgac gaacatgcgc aaagttaaaa
    22741 tgaacgtcca aggacgcgtc caaggcgtag gatttcgcta catgaccaag atggtcgctg
    22801 atcaactagg cgtgactgga acggtaaaaa acgaagatga cggctcagtc acgatcgaag
    22861 caatcggcaa tgacgacatc atccagaaat ttattgaaga agtcaagaaa tctccgagcc
    22921 caagtggacg agtgcagtat gtggatgtcc aagaagaccc gatgattgag gaacgaaaga
    22981 aatttgatgt ggtgggataa ggttgtgaaa aagctggtct gcatcaagca ttaaggacag
    23041 gttgtgaaaa atagctctcc atatttttca acaggctgga cttaatgccg tagatgcaag
    23101 cttttgaaca ccgtcaataa ggttgtgaga aagtcgctta gttttgagta gtaaggagaa
    23161 ttttgtgaaa atggctttcc acatttttgc ataaaatttg acttaatacc gagaaactga
    23221 cttttgaaca ccgtcgataa ggttgttttt gagacgcttt gcctcaagta ttaaagatag
    23281 gtagtaaaaa agctgatcta taacttgcaa taaatttgct taacccatat tttccagaca
    23341 gaacaaaaaa gtttttagtt ttttaaataa gaaagaaaca cttggaatca ctcttttctt
    23401 tccaagtatt tctttcttga ttttttgtct ttttttcaaa atttttcgct acgtaaaaga
    23461 acgaatattt atatgaaatt tctatgaagt tgattgaatt ccatgcgctt ttttagtata
    23521 gttaagtttg tgtaaaaatt atagaagagg aagtattcta tgaataaatg gaaaaactgg
    23581 ttattgggat ccggacttgt cagtatcctg ctctttttat ctggctgtgt gcggatggat
    23641 tcaaacggaa accctgacac ttcgggaatc gtttatcgag tactcgtcca tcctatgggc
    23701 caagcgatta catatctagt tgagaatttc aattggtctt atggctgggc agttattgca
    23761 atgactgtga tcgtccgtat catcatcttg ccactaggta ttagtcaatc gaaaaaaaca
    23821 atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag ttgaagcagc ccaacaaaaa
    23881 ttgaaagctg caacaacacg tgaagaacaa atggcagcac aagcagaaat gcagcaagta
    23941 taccgcgaaa acggattgag tatgaccggc ggaatcggct gtctgcctct attgatccaa
    24001 atgccgatct tctctgcctt gtactttacg gcacgttata cagaaggtat tcgggaatca
    24061 tctttctacg gcatcgattt aggctcacca agtatggtac ttgttgtcat tgcaggggtc
    24121 gcctatctgc ttcaaggata catctcaacg atcggtattc ctgaagaaca gaaaaagacg
    24181 atgcgtacga tgctaatcgt ctctcctgct atgatcgtct tcatgtcgat cagtgcgcca
    24241 gccggtgtta cactttactg ggtagtcggt ggtgtgttca gctgtctgca aacattcatc
    24301 acaaacgtta tgatgaaacc acgtttgaaa gcacaagtgg cagaagagtt gaaacaaaac
    24361 ccgccaaaac aagtcgtcac tccaagaaaa gacgtgacag aatcagcaaa gccagctaac
    24421 aacaaacaag caacaaaaca aatcaaacaa ccaaaaaaca gtacaaacaa caaaggccgc
    24481 aacgcaggaa agcagcagaa gcggaaataa aaacatagga caaaaaagat gagagctaag
    24541 ccctcatctt ttttgttact cttcctcttc tgctattcca ttaattaatt cgttgacaga
    24601 gacaatgctt tcacatcctg cacgaacaaa ctcgggctct gaaccaatca tttgtgagtt
    24661 gataaattca atgaccatcg gaagattcat cccagcataa agagtaatct gttgtccttc
    24721 cattattagt ttagaaatga tatttgcagg tgtaccacct aaaagatcgc atagtaccac
    24781 aaaatcattt aacgaatctg tgacagtgag aaacttttgt ttgtatgttt cagtgccatc
    24841 ttctggtaat aaaggtaacg tgtagatatc ctcttgaggc cccatgatca tttctgtact
    24901 ttttttcaat tcctcacaaa attttccgtg actaatcaat accaattgtc tactcatttt
    24961 tcgctctccc ttatttcgaa atcacaccca atgcagataa agcaacacac acaattaata
    25021 cgataaaaat cgctcgagta ggagtcatgt ttttctttcc taataaccaa taaacaccac
    25081 cgacgatccc agcaggtaaa attttaggta agatcatatc gatatagttt tgcatattca
    25141 atgtcacatc gccaatcgtt ggtgcccatg aaaatttaac attgatcatt gtcgcaacta
    25201 atgcccctac catgaatacc cctaataaag tggctgcgtc cgttaatgca gttaagcgag
    25261 attgcatagt agtaactaag gaaattcctt ctttgtatgc aaattcaagt tgcttccaac
    25321 gaaaaaccat gacaacgatc tgagcaaaaa tccatataaa cacacctatc ggatttccat
    25381 cagttgccat attcgccgcc aaagccccaa aaatcgttgg aatcaaagca gcaaatacgg
    25441 aatctccaat cgctgcaaag gaacccatca atccagcttt cagccccgat acggtttcct
    25501 ttgaagcaat tccctctttt tcttccattg caagatcaat cccagtaatg attgtattaa
    25561 aaaaattaga agtattaaaa aattgcgtat gtgttttcat catctctttt aattctggtg
    25621 tattgtctcc atatattttt cttagttgtg gcaagatagt atataaatag ccagaaccct
    25681 gcattctctc atagttccaa cctaattgaa aagtgaacaa acttcttcga ttgatttgct
    25741 taaaatcttc ttttgttaat ttgtaattag atttcgtcat cttcgatttc cccctcaaca
    25801 gaatgtgatg gctcactagc taatggttga tggtctttgt tttgatcatt cttataactg
    25861 atcattgcta gtgctagacc aatcaatgct acagcaagca tcggcagcga attgaaagtt
    25921 gccggaagat ctttaacaac gcttgccaca cttgttccta ataattgaac attagagaat
    25981 aacgttgcta ataatgccgt gatcgtaaat cctaaaataa gataagatag atgttttttt
    26041 actggtaaat agcgcagaag aatggcaaac ccaacagctg gtaaaacagc tcctgctact
    26101 gaaagtccat cgcctagcca ttttagattc ccgtttaaaa cagaaacaac attttcaact
    26161 aatccaccac caaatgctaa agctaaaaat acaggaatca tacgagagag cgaccaaggt
    26221 aaagctccca ttaagaaatt gcgttcgatt gcttgataat tcatgtcttc tacgtgacgg
    26281 tcaatacgat gggcaaagta tgtgttagcg aatctagcta aaatatccaa ttggatcatt
    26341 aagcttgcta caggcactgc aatagcagca atcgcttgtt ctggattcat tccaagagaa
    26401 atcgagaagg cagttgctaa aatcgtccca gagttggcat ctatttttga tgcgcctcca
    26461 aacgtcccca ctcctaaaac tgtcaactgc atactgccac caataatcaa acctgcgttt
    26521 aaatcaccca ttactaaacc tgcatataac cctgcaaaca ctggcgcact cattgaagaa
    26581 tagatctgta attcatctaa aatttgataa cctgcatata atgttaataa taagatctgc
    26641 caccattgaa tatccatgga tcattcctcc tatttgatta aaagattcat aaagtcttct
    26701 gctttatcgt ttggtaccat ttgagcaatt aaatgtatac caagctgatc taatttttta
    26761 aatacttcga tatctgcatc taccacattg attgattttg taatggaccg tgtttcattc
    26821 gtttgtgaca tattccccac atttacttct ttgataggaa ctcctttttc tactaaagat
    26881 aagattcgat ctggcttttt tgctacgatc aatactcgtt gagagtcata tttgccagct
    26941 aaaatatttg ttgctgcttt ttcgattggc aaaatgctta attttacccc tgctggagtc
    27001 gctaacttca atccactttt ttcaattgtg tttgtggcta cctcatcatc cacgaccatg
    27061 attcgactaa tattcagttt cgttgtccaa agatttgcta cttatccatg tatcaaacgt
    27121 ccatcgattc ttacaccaat aattgccatc atgcattctc cttatctatt ttttctattt
    27181 attgttcaat taaaactgtg acatcatcaa atatcccttc tgtttcaaag ataacgtttt
    27241 cattttttct ttcttatatt tcatttttaa gctaagaaca aattccttcc caactaaaga
    27301 aaacgctttc ttttatattt aagttcattt gattttctta acttgaatgt gtttcatcaa
    27361 catcgtaact ttcatagggt cgatccagcc agttttctct tgttgcgcgt ttgccatacc
    27421 acaagccata gaaaatgcga gaatctcttc gattgattgc ctttttgata acgcatgggc
    27481 cgatcctgca agggtcgcgt cacctgaacc aacaggatta acaactgata ttttaggaat
    27541 aatgacttcg taaaaacact gagcaacttt tgccacagca cctctctctc ctaaagaaac
    27601 aacaacccat ggcactcctt caaatattgg atgagctaat aaggactgaa ttttttctaa
    27661 ttgaatcgaa gaacagatac gttggccaat cagttctcct aattcttcca gattgggctt
    27721 gataagtagt ggcttctcct taccttccaa tacttcaagc aaaaatttac cagaagtatc
    27781 tagtaatatg tctttccctt ttttatcagc taaagcaacc aattgcttat agtaatcaag
    27841 agggcatcct tggggtaaac tacctgagat cgtcataata tctgatattt cgaggagctg
    27901 ctcgaaatga tcggtaaatg tctgttgttc tttctttgtt atgaaggggc ctcgttccaa
    27961 aagctctgtt tgtctcgaac tatgcaggat agcaatagaa tcccttgtct ctcctttaat
    28021 tggaacaaac tgatgcatga tccctaactg atctagctgg ttctttagat attcaccgtg
    28081 ctggccacct attaaaccag tagctatcac ctcttctcct aataaattta gaaccctcgc
    28141 cacattcact ccttttcctc ccgctgtttt attgactgtt tcaactcgat tgactttgtc
    28201 gaggtttaat tcttcaattg tatacgcgat atcgatggaa ggattcaagg taactgtcgt
    28261 aatcatttca tcttcacctt tttgcttttt gttttattga accaaggggt agcggtttca
    28321 tttattactt tatttaaagt ctcgatgttt tctctcccat ttgttcttaa ccaatgaata
    28381 gctccgtctt ttccattttc gacgtatgct ttgaccccat ctttccatgt cgcacgtcca
    28441 cacaatacac cattgaaggt ggaacccgat tcatgagcaa aaatcagtgt ttgttgaaat
    28501 aattctgttg aaacaccccc actcaaaaaa atgaatggta atggagtcgc ttcactttgc
    28561 tccttaaaga aggcagcagc ctcttctttg ctgtgaataa attcacctgt ggaatagcca
    28621 ttcacataat tcatattgac tggtacttct actttcaaca catcaacttt atattttgga
    28681 tcagaaaatt ctttcatcat ctcaataact ttgtggggct tgactttcgc atactctgga
    28741 gaagaactat cgcttatttg tgcgtcataa gaaactaact ccaaatagaa gggacgattt
    28801 tctccttcgc actcactacc taaacgttcg atgaacacat gcttcaaatg attgatttcg
    28861 ggtgcttcat caacatcgta gtaaagtaag aattttactg catccgcttg ctgttctttc
    28921 aatctgagag aagaccaatc cgctaataaa tcaggtagac gaccaggcgt agttgcgtca
    28981 tagccggttt tctcataagc taacaataac cccgtctctt ttgctctgac ttttgctgct
    29041 ggcaatccat attccggatc caataaaata gcagaagcat agggtgtcag ctcagctgat
    29101 actagttctt tgaattcact gatttggtct tcacttggct cttttcccaa cgatttgatc
    29161 atttttttta gagcaccacg ctgatcgatc gctaatgcac taataatccc atcttttgtt
    29221 gatagtctct ccattgccct atttttttct tcattcatct gtcatttctc cttcggtttc
    29281 atacttatga ataatgacgc ccttgactac tcgattgacc gtgcctgttg gagatggtgt
    29341 atctggttta ttagctactt tgattgaagt atagagtgca atgatttgtg cagtcataat
    29401 catcgctaat gcaagatagg catcgggcaa tttactcacc gagtcttcca atagcacagt
    29461 catacctgag aaatcaatag tacccggttg cgcaatcgca atgacattgg cagtgacccg
    29521 atcttctttt atttcctcta aaatatctaa gtcataattt cgcgtatatg gttgattgct
    29581 cacaaaacct acaacgagtg tctcgctatt taaaaaagac tttggtccat gacgaaaccc
    29641 catcgaagaa tcaaaaattg tagcaacttt acctgctgtt aattctaaaa tttttaattg
    29701 tgcttctctt gtcgcacctg ccaaacttcc tgaacccaga taagcgatcc gttcaaaaaa
    29761 atcaggtaat aacttttgaa tttcttgatc tcttgaaaga agatcttgac cgattttaac
    29821 taattgttca agatatcttt cttttttctc cattgctgtt tgatcaaaga tccataaagc
    29881 actcaataac atacacgtaa aacttcctgt catagcaaaa cctgcatcgt ttgctctctc
    29941 tggcatcaat aacagataat tctgtttatc atcctgtgcg ttttttgcca acatcccctc
    30001 tttagcacaa gtaatcgtca aatgaaacaa tgtttcaact tgttcatttg caatttgcac
    30061 agccgctaca ctttctggac tgttgccact gcgagcaaat gaaactaata aagtcggttc
    30121 atttggaaac aaaaattctt ttggtgaagc tacaatatct gtcgtgccaa aactgtggaa
    30181 gataaaaaac tgattgtctc catgttcatt tagatagggt aagagcgtat ccccaatata
    30241 ttgagacgtt cctgcacccg taaaaataac acgtattctt cttccgtttg cttctttttt
    30301 tatttgatca aaaaatagag ctaaattttc ttggctttgc atcaatctat cgattgtttc
    30361 tttccagaga tctggttgtt gttttatttc ttgtgtcgtg attgctgcac ctatttgttt
    30421 taatttgttc ttcgatagct caaacatgtt gttctccctc tcaatcattt tcggatatgc
    30481 cgaattttat aatgaaactg gtccgctctt gctacactat ctgtatattc gataatctca
    30541 tttttcacgt tataggttat cctcgtcaga tgtaagacgg gagcgccatc agcgattcgt
    30601 agcacaacag catctttttt ttgcacgata cttgcataaa actcttcatc agctaattga
    30661 acttgttgtt cataatcctg tgaaaacaca tcatacaacg gtttactttc aagtaacggc
    30721 aaatcaagtc ccataaatag tttcgccggt agataagaac gctcaaccat caatggctct
    30781 tcatctgcca aacgcaatct tttaagttta aatacagttt cacctaaatg gatgttcaaa
    30841 ttcttagcaa ttctcttatt agcttccatt tgtctaaatt ctaaaatgac cgtcttaggg
    30901 acgcgcccta tttcgcgcat ttcttctgta aagctatatg ctccagctaa gttcgatgac
    30961 ttatcagaca aatcagagac ataggtacct tttccatgtc ttcggtaaat ataaccttga
    31021 ttttccaatt cctgtaaagc cagacgtacg gtggtacgac tcaccccata gtgttgacaa
    31081 agttctcttt cagataacag cttatcatgc ggagtcatct gtgtttcgat acgatccttc
    31141 aataaatcaa ctagttgata atacaatgat tttcctttat ttgagctctc cataaaaatc
    31201 acctcttctt tactggttat aaccacaaca caagaatagt gaattattaa tcatttgtca
    31261 aaggtttttt agaccaaaaa agctattata acagctcttt aaagctttat atcttgaata
    31321 gacaaagata cctcaaaaat accagttacc aataaaagaa cagaaaaaaa gacgaaccac
    31381 ataagcgatc cgtcaaaatg gtctaactta tcgagcagcg tgatccacat taatccctat
    31441 gatattccta caactatcgt tttattactt ccaatatcat ttacaacaac taaaaatagc
    31501 ttattaatga caaatgttat tattagtttt agataacttt tgcagtttta tacttttttt
    31561 ctataaaatt tgacaaacat ttttttaaat aactaattat tttttgaatc tgtcactcag
    31621 acaatccttt ctcttacttg ttaacataaa tagaagttgc tgtttcttct aatagtgtac
    31681 ttaattcttt aatattttgt tttccttctt ctaacatcca actactcgct ttttcttcgc
    31741 cttcggtaac aaaaggtgct actcccttcg cccatgttgc tcgcccacat aagacaccat
    31801 tgaatgtcga acctgattgt tttgcaaaac gcaatgtatc ttgaaatagt gcagcgctga
    31861 ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca tagaaatacg
    31921 cggatgcctc tgcttgacta taacaagtat cttgcccata tccttccaca aatctcatat
    31981 tcactggtac ttctactttc aatacatcca cttgataacg ttcttttgaa aattctcgca
    32041 tggcttcaat cactttatgc ggttttactt ttgcatagtc aagagaagac ggatcgccaa
    32101 tagttgcatc gtaagaaagg agctctaaaa agaaaggtat gccttctgct tcgcattctg
    32161 aaccaatccg ttctataact atttgttttt gcacattaat ctctactggt tcatcaacgt
    32221 catagtacaa taaaaattta caagcatccg cacccacttc ttttaacctt ttcactgacc
    32281 aactattcag taaatctggt agtcggcctg gcattttcgc atcatagccg gttttttcat
    32341 aagctagcaa caaaccacat tctttctctc gaagcgctgc tgcaggcaga ccatattctg
    32401 gatcgagtaa aatagcggaa gcatacggag tcaggtactg agaaaccatc tgtttaaatt
    32461 ggatgatatc ttctgatgta gcttctttat attggttgat cattttcttc aaagcaccac
    32521 gttggtcgat agctagcgcg ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt
    32581 cctgtttttc tatactcatt ttcttcatat atcttcacct atttctttag ttaatattgt
    32641 agctatttct atttttgatt ttgcagctct caactgttct gtaacctctt ttcgcatcaa
    32701 tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg aatctgggat
    32761 aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt caattccttt
    32821 tttcagtgtg acgataatca aactaggttc attgattgtt tgagacttcg catgagggat
    32881 agcaaaacct gacattattc ctgtagtacc ttctttttct ctttccagaa gtttttgata
    32941 tacttctttt tcgtcatgag cgattcctaa acagaatgtt tgctttgcta aaaacttaaa
    33001 tgcttcttct tgattatcta atggctgttg taaaaaaatg ttctcttcat gaatgattgt
    33061 catgataaac ctcctgctct tccttcttcg ataactgtac gatttgttgc tttattatcc
    33121 ctcttcttgt ctttatcagt tactcttttc ttttggatca aacctagtat cactccacca
    33181 acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac tacaggtaac
    33241 actaaaaaac cgccatgtgg ggctggtact tggacaccaa acagataggt caagacagca
    33301 gcaattgatg agcctaacat catcgttggt aatactcgga tcgggtcttt cgcagcaaac
    33361 ggaatagctc cttccgtaat atgagttgat cctaaaataa aattaaccaa acctgcatta
    33421 cgatcattag tatcaaaata tttaccaaac aataaagtag caaaaccagt aatcaacggt
    33481 ggtgcaatgc aagctgctga cgcaccagcc ataaaagtcg tgtttccttg tgtcaaaaga
    33541 gctgttcctg ttacatatgc cgccttatta accggtcctc ccatatcaaa cgcacacata
    33601 cagccaacaa tcacgccaag aacaagtgga ttcgaatatt caaatccagc taaaaagctc
    33661 atcattcctt catttatagc cttcattgga cctgccagca aagacatcac gatgccaata
    33721 aatgctacac caataactgg atataagacg attgccttca agccatctag ttgtttaggt
    33781 aaaattatca gtagcttttg caaaaataag ataaaataac ctgctaagaa cccagctaaa
    33841 atacctccta aaaatccagt accgccattc atggctacga ttccaccaac aaatccaacg
    33901 actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac tggaaccatc
    33961 attcccattg ctgcatcgtc aactgctttt accattgccg ctgtagcatt gtattgtgca
    34021 tgatctggat cagaagaata gattccccaa acggcaaatg aaatagcaat caatactccg
    34081 ccagctacca cgaaaggcaa catatgagag acaccgttca tcaaattttt atagatgcta
    34141 cgaccaaaat tagcaccact tatcgcttct ggttctaaag tatcagcagc ttcattatct
    34201 gctaagacag taccttttcc tgccaatgct tcgtcaatca gccgttgtgc atccttgatt
    34261 cctgtactga cagaaacatc aatgactcgt tttcccacaa atctttctga atggacatct
    34321 ttgtctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt taattcattc
    34381 tcgatcccaa tttgtccatg ggtctccact ttgatcgtga tgcctttttt cctagctgct
    34441 tgctctaatg cctcttgcgc catgtaggtg tgcgcaattc ctgtcggaca accggttgct
    34501 gcaattagtt gatattttcc catctttcga acctctcttc cttgattttg atttgtttac
    34561 ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcagta gagctgcctg
    34621 ctgctatgct tttacgtaac gtttcatcaa gcgatcgtcg attcatgtat cctgctaaaa
    34681 aagtgcctaa catggcatca cctgcacatg ctgtattgac aacttttcct tttggcgagt
    34741 ttcctcgaat caccctgtct ttcgtaaaaa gaatcgctcc tttctcaccg agggataaca
    34801 aaacattttc tgcgcctgac tctactagtc gttgtccata aacaaagtaa tcttcatctg
    34861 taaccatttt tcgtccgaac caagaagaaa gttcttcctc atttggtttc agcaaaaacg
    34921 gtcgataggg taaacaatcc atgacttctt gatcactact atcaataatc aaacaaatcc
    34981 gactgatttc tattaaaacc tttggagaca gccctcttgg taaactccca gaaacacaaa
    35041 gataatcccc tgcttgtaat gctcttattt gatttaaaaa attatgaaca gctgtctgcg
    35101 gtatttcagg tccttgattc actaatttgt attcttctcc cgtttgattg acttgtgtaa
    35161 atacattgat acgcgtcata ccaggcactt caataaactc tgtttggatt tgtttctgct
    35221 gtaaatagtc ttctatatac tttcctgtga acccagcaga aaaacctaat gccgtacttg
    35281 ggaaatctag catctttaat actagtgaca cattcacccc ttttccgttt gcctgaatgt
    35341 catcctcttt tgtgcggttt accataaaag gatgcatctc atctgtttca ataaatagat
    35401 caattgccaa attcatcgtg catgtgtaga tagacataac caacgacctc cttgattcta
    35461 ttatgaaata cgcgaaatag aaaatgtttt cttaatcgtc ccaacacaaa gaaaaccttt
    35521 acatttataa actcgtcgtg ctttttttaa ctaacttttg gtgaaagaag gaaagaatat
    35581 gaatcacaaa ttaagtgaaa cagagcagta cctctggaac tttatcgaac acctcatcct
    35641 agagatccct aactattcca tcgtgaaact aagtgaacaa gcaaatgtct caacagccac
    35701 cattgtgcgg acaatgaaaa agaaaggcta tgaagggttt acttcgttta agcatcatct
    35761 aaaagaagga gaaaacaaga acatcaattt ttcattttta gataaagtag acaaaggaat
    35821 ccgccgagcc attctaaaaa atgaacaaga agtggtacgt acaatcaata tgttggaaat
    35881 cgggaacatt gaagatgcaa tacaaagaat caaatttgct gaccgggttt ttgtctttgc
    35941 ccgaggattt tcagaaatga ttggccaaga aatgctggta aaacttcaat taacgggaaa
    36001 aaattgtcag atgcatactg atccagaaat tatcaaaagc atcagtcgga aactaactaa
    36061 gaaagacagc gttatttttg tttctttgaa cggtgagaaa acaaataaaa acatcaaaaa
    36121 taagatttat gtctttattt ttcaattttt taaat
//
LOCUS       DACEOM010000025        35822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEOM010000025 DACEOM010000000
VERSION     DACEOM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.026"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..1282)
                     /locus_tag="IW781_001163"
     CDS             complement(5..1282)
                     /locus_tag="IW781_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182171.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="IW781_001164"
     CDS             complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="IW781_001164"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1182172.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2204..3148)
                     /locus_tag="IW781_001165"
     CDS             complement(2204..3148)
                     /locus_tag="IW781_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1182173.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3150..3836)
                     /locus_tag="IW781_001166"
     CDS             complement(3150..3836)
                     /locus_tag="IW781_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1182174.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4310..4630
                     /locus_tag="IW781_001167"
     CDS             4310..4630
                     /locus_tag="IW781_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1182175.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4784..5689
                     /locus_tag="IW781_001168"
     CDS             4784..5689
                     /locus_tag="IW781_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1182176.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            5686..6459
                     /locus_tag="IW781_001169"
     CDS             5686..6459
                     /locus_tag="IW781_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1182177.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(6640..8064)
                     /locus_tag="IW781_001170"
     CDS             complement(6640..8064)
                     /locus_tag="IW781_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1182178.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            8275..8721
                     /gene="nrdI"
                     /locus_tag="IW781_001171"
     CDS             8275..8721
                     /gene="nrdI"
                     /locus_tag="IW781_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1182179.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(8786..10768)
                     /locus_tag="IW781_001172"
     CDS             complement(8786..10768)
                     /locus_tag="IW781_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1182180.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="IW781_001173"
     CDS             complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="IW781_001173"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1182181.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(11681..12742)
                     /locus_tag="IW781_001174"
     CDS             complement(11681..12742)
                     /locus_tag="IW781_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1182182.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(12953..13480)
                     /locus_tag="IW781_001175"
     CDS             complement(12953..13480)
                     /locus_tag="IW781_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1182183.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(13547..15049)
                     /locus_tag="IW781_001176"
     CDS             complement(13547..15049)
                     /locus_tag="IW781_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1182184.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(15307..16428)
                     /locus_tag="IW781_001177"
     CDS             complement(15307..16428)
                     /locus_tag="IW781_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1182185.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            16554..17495
                     /locus_tag="IW781_001178"
     CDS             16554..17495
                     /locus_tag="IW781_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1182186.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(17645..18541)
                     /locus_tag="IW781_001179"
     CDS             complement(17645..18541)
                     /locus_tag="IW781_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1182187.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            18810..20150
                     /locus_tag="IW781_001180"
     CDS             18810..20150
                     /locus_tag="IW781_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1182188.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20368..20811
                     /locus_tag="IW781_001181"
     CDS             20368..20811
                     /locus_tag="IW781_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1182189.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="IW781_001182"
     CDS             complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="IW781_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1182190.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(23136..24359)
                     /locus_tag="IW781_001183"
     CDS             complement(23136..24359)
                     /locus_tag="IW781_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1182191.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(24390..25229)
                     /locus_tag="IW781_001184"
     CDS             complement(24390..25229)
                     /locus_tag="IW781_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1182192.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(25246..25926)
                     /locus_tag="IW781_001185"
     CDS             complement(25246..25926)
                     /locus_tag="IW781_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182193.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(25928..27001)
                     /locus_tag="IW781_001186"
     CDS             complement(25928..27001)
                     /locus_tag="IW781_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1182194.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(27648..28319)
                     /locus_tag="IW781_001187"
     CDS             complement(27648..28319)
                     /locus_tag="IW781_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1182195.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(28406..31084)
                     /gene="mgtA"
                     /locus_tag="IW781_001188"
     CDS             complement(28406..31084)
                     /gene="mgtA"
                     /locus_tag="IW781_001188"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1182196.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            31743..32180
                     /gene="ndk"
                     /locus_tag="IW781_001189"
     CDS             31743..32180
                     /gene="ndk"
                     /locus_tag="IW781_001189"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1182197.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(32202..33068)
                     /locus_tag="IW781_001190"
     CDS             complement(32202..33068)
                     /locus_tag="IW781_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1182198.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(33228..35321)
                     /locus_tag="IW781_001191"
     CDS             complement(33228..35321)
                     /locus_tag="IW781_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1182199.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        10369 a         7523 c         6320 g        11610 t
ORIGIN      
        1 ttttttatag taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt
       61 ctaacttctg ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc
      121 ctttccattt gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt
      181 gccatttttc ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt
      241 ctttacgcaa tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc
      301 cttctgtaaa accatagcga caatattgct tccaaataaa tagatagatt tttggttgga
      361 tgaatcgttg gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac
      421 gttttgtctt tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca
      481 cttcttcata tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga
      541 attcttctac atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct
      601 gtcgcttttt tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg
      661 cttgataatc cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag
      721 ctaattttat atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac
      781 agtatttttg ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt
      841 cgtctaaggc cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag
      901 agacatgcag gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg
      961 gcgatttaac gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct
     1021 ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg
     1081 cagctaaata atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga
     1141 ctagttcccg ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt
     1201 attgtttaga aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa
     1261 agacggattg ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta
     1321 ctaacataac gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat
     1381 ttccggaaat atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag
     1441 caatgtcttc taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg
     1501 agaatggcac gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa
     1561 gcaaacgata catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg
     1621 gtagaccatg atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag
     1681 cggctggacc gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga
     1741 ttcttgcttc tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg
     1801 cttgtccgac acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt
     1861 gactgctttt ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt
     1921 catctggcat tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat
     1981 caaaacctgt tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg
     2041 ttatctttta tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat
     2101 aacataacag atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta
     2161 gaagaggttg cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca
     2221 tataagcaat ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc
     2281 cgtatttgtc tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg
     2341 gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat
     2401 tcgctccatg actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg
     2461 caaatgcatt ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc
     2521 cgatgttcac atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa
     2581 ctccagcgct tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg
     2641 gccctactgt gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc
     2701 gagaaaagaa catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag
     2761 cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa
     2821 taaaaatttt cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc
     2881 ggctttctcc tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc
     2941 caccgttcac gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc
     3001 agaaaaaggc tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag
     3061 tgtaaatcaa tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata
     3121 aaatatcagg atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc
     3181 gctaatttct tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc
     3241 actggaactt ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt
     3301 gttgattgga ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag
     3361 cagacatttt ccatagctga agccatacct tttagttctt cttcatgctt aggatggact
     3421 tctaaccctt ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat
     3481 ccagcaactc caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa
     3541 atatctacaa tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat
     3601 tctttcagac catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc
     3661 atcccgcgat tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga
     3721 ccgccagtca aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta
     3781 aatgcaaacc cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat
     3841 tttcctccat aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag
     3901 ttgttttctc acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa
     3961 aattatctta cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga
     4021 aataaggtct agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa
     4081 attgcaaagc attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt
     4141 tactaaaaag tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata
     4201 tacaatctgc caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat
     4261 gattttcctc tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat
     4321 ggaattaatc atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga
     4381 tggtttttct aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta
     4441 ctttttgtca aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg
     4501 gattggcgca gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct
     4561 ttccacttgg ttctttgtgg ggatgctact catcagtatc attggcatca agattactag
     4621 cggccactag acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat
     4681 tcaatttttt tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat
     4741 caggtgtatg cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt
     4801 ccaagaatta tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt
     4861 atcccctggc aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat
     4921 gaaagctatt ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga
     4981 gatttctccc tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg
     5041 tatctatcca ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt
     5101 atctgctatc gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc
     5161 gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca
     5221 tccgtctatc gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga
     5281 tgtgcgaaaa atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca
     5341 cttactcagc gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat
     5401 catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc
     5461 tacaacaaat gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga
     5521 tcctgataaa atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct
     5581 attgtctgca caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc
     5641 ttctctattg gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta
     5701 aaacaagaat tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta
     5761 gttgttttgc tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca
     5821 gttgaaatga ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca
     5881 gctgcaagca cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat
     5941 cgaaagtatt ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct
     6001 gtttatttat accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct
     6061 tcgatttcct tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact
     6121 tatactttgg gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct
     6181 tgctttatca atagttccgg cgcatccatc tcagcaggaa tcgtctttta ttttgcttcg
     6241 tcaatcatat ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat
     6301 ccgatcagta tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg
     6361 actcatcttt ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta
     6421 gcattaggat atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct
     6481 gtgaaagaga cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga
     6541 aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac
     6601 gacaggctca caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt
     6661 ggcaaataaa accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc
     6721 taagtggata tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat
     6781 gcctggcaag aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc
     6841 ccatacttga aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg
     6901 ataagaaagt ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt
     6961 accaagtgtg taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac
     7021 attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt
     7081 tccccatgct ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa
     7141 gatcgtccat gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac
     7201 tgctccccat ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat
     7261 cgtttgcagc tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat
     7321 aataatcaag ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc
     7381 tcgtgaacct acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat
     7441 gcaactcaat acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc
     7501 gctagaaaag ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa
     7561 taaaaataat ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc
     7621 gactccccga ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga
     7681 tgccagattt tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa
     7741 tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa
     7801 accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg
     7861 acgtttttta ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag
     7921 tacccaactg aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa
     7981 tacgccacct ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc
     8041 ttttttctgt tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa
     8101 aagtataaca aacctttttt atttttactt aattaataaa aactttaata gaaatataat
     8161 tctaccggct tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca
     8221 ccaaaaaact taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac
     8281 attttatata tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat
     8341 gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat
     8401 gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta
     8461 gacggcggaa atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa
     8521 tatctggaac atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa
     8581 aacttcaata atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct
     8641 ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt
     8701 ttagcagaaa acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc
     8761 tttcgtttct attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt
     8821 ttgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga
     8881 cgatcatgga tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa
     8941 gcgtatcagg tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta
     9001 agtcaactgt gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc
     9061 caatagcgtg tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat
     9121 gtccgttttc tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat
     9181 cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac
     9241 tttcagctga agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag
     9301 tcgctaattc ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga
     9361 agccaccagc ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga
     9421 tcaatgggat caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca
     9481 ttccgatcac tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta
     9541 gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag
     9601 ctcctttgat catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg
     9661 ctgcacccac ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag
     9721 tttcatcaat catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca
     9781 tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc
     9841 agttgatccc accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa
     9901 tttcaatcag accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat
     9961 caggtactac tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga
    10021 cacctaggat cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg
    10081 gtgcttccgg attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg
    10141 gtgttgcacc aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca
    10201 tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg
    10261 ttccggctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga
    10321 caaatgcagg aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt
    10381 tagatggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac
    10441 ggtcttttcc agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc
    10501 ctaatttcac gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc
    10561 cgatgatgac ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta
    10621 attcagagac atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt
    10681 ggacaatgct ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt
    10741 cttccaaagt cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca
    10801 aatccgtttg cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc
    10861 atgacgatag ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg
    10921 taatcagctt ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt
    10981 gttggtttga catcgaccat caagttattg aatactttac ctataccaat catactgata
    11041 gtagaaatca tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct
    11101 gtcaaaaatt ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg
    11161 gcttgtttat tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat
    11221 ccaccgatca catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt
    11281 gtcaaattca gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg
    11341 acagctacag tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct
    11401 acaccgaatg tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg
    11461 gctcccatat aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact
    11521 ggttcgatac ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc
    11581 attagttgaa cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg
    11641 cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta
    11701 taaattgata gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa
    11761 ctttagctac tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct
    11821 cacccatata gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag
    11881 ctgtatttct tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga
    11941 catctcttgc atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt
    12001 catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct
    12061 cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta
    12121 atagatctag agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc
    12181 cttgatatag tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac
    12241 cttgaatttt gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg
    12301 gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca
    12361 attcatcgat atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg
    12421 accagtcggc aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa
    12481 ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg
    12541 aaatatcgac catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc
    12601 gcttttgata tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa
    12661 agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca
    12721 taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg
    12781 actagagtat atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc
    12841 atttctaaag gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc
    12901 taagcaaaag cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat
    12961 aagaaaccaa ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat
    13021 gaaaataagc attgatcact cttatcaagg cattatggca aactaacaga tagacatttt
    13081 cttcgtcttc gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg
    13141 actccccatt tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt
    13201 tgcttggcag tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca
    13261 gcggcaagct gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg
    13321 aatggatgat tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg
    13381 cctgcatctg tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg
    13441 cgttccaagt tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg
    13501 caaagttccc cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa
    13561 gagaaataat ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca
    13621 tggcaggttt cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat
    13681 aattcaatgg ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt
    13741 ccactttttt ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt
    13801 ccatcccaag ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg
    13861 caaattccgc caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca
    13921 gatgttttgg ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat
    13981 ggataatgga tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct
    14041 cgattgtaaa atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta
    14101 cgcttaaaag tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt
    14161 cgatatcttt taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg
    14221 ctttggatcg tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc
    14281 tagattcagt atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag
    14341 accattcggc tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt
    14401 cattttgagc catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat
    14461 tcccttgata aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat
    14521 cgataaatcc ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag
    14581 ccgctacttg ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga
    14641 aatgagtgtc atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata
    14701 gtgtgctgaa ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt
    14761 cgatttccca tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt
    14821 tatcttttgt ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat
    14881 aaggtagttt gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga
    14941 taagtcctag tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc
    15001 cttggctgtc gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc
    15061 tcgtaaaaaa agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata
    15121 cacacaaaaa gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct
    15181 tccttgtctc cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa
    15241 tgcacggatc cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt
    15301 tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt
    15361 tttttctaat gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat
    15421 acttaagaca attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca
    15481 aatagcgatt attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt
    15541 tccgtggatc gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc
    15601 gatctttttt tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc
    15661 tcccaacaaa aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc
    15721 taaatccacc tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa
    15781 gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg
    15841 ttgacctgta tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag
    15901 aacgtttctt cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta
    15961 cccttctaaa gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct
    16021 tcctcctaaa gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc
    16081 tcctaaggca taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc
    16141 taaccccaat gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa
    16201 taaatagctg atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt
    16261 tcccctgagc gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac
    16321 attgattttt cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa
    16381 tgaagaagca cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca
    16441 ctttcttctt aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct
    16501 tccatttttc tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa
    16561 tcgcaatagc tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg
    16621 gaaatgaggt cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg
    16681 ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag
    16741 aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc
    16801 aattagatga tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt
    16861 gtttactaaa tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata
    16921 tcttatttgg gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt
    16981 taggtgatgg agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa
    17041 aagttgtaga agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt
    17101 attccatttg gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg
    17161 attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg
    17221 tatctgaatt tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt
    17281 accaacatat tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt
    17341 accaagacct gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt
    17401 ggcgaaaagg acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag
    17461 tccatgcaaa agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg
    17521 aatcagcagc tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc
    17581 tcatgatgcc ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg
    17641 ttttttattt gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat
    17701 cgttagtgat aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata
    17761 ggattggctt aaatggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa
    17821 gcaaaaaaat attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca
    17881 ctagtttctt ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct
    17941 cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag
    18001 taagatatcc aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat
    18061 ctctttttat aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg
    18121 caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa
    18181 atgaactatt atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct
    18241 catcgattgc cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt
    18301 gattccaaaa aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct
    18361 ccatatcaaa tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag
    18421 ctgatttaat gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga
    18481 tggaaggcaa aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca
    18541 tcttttccgc ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa
    18601 agccagtaaa acgtttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg
    18661 aaataaatca ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct
    18721 gaaaatttgt tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg
    18781 attgtgaatt taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta
    18841 gctccagaca tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag
    18901 gaagtggcga agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg
    18961 ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca
    19021 cgcatcacgg attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta
    19081 ctgattgggt ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg
    19141 cttccttttt gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg
    19201 ctgttttaac tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt
    19261 ggctggcttt tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg
    19321 tcggtcagca aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg
    19381 gtgggtttac tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt
    19441 cttacggcgg ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg
    19501 cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt
    19561 tgtttttgct tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt
    19621 ttgtgatggt atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga
    19681 ttgtccttgc tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt
    19741 tcttccgttt gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc
    19801 aagtaccgca acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt
    19861 cttattttgt cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat
    19921 tattgatctg gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat
    19981 tatgggataa aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta
    20041 tcctgttcac gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt
    20101 atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga
    20161 aaattttatt ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa
    20221 gtcttgttgc aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt
    20281 caaaaaccct ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa
    20341 ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc
    20401 ggtgtttcaa cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat
    20461 atcttcttca gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc
    20521 atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta
    20581 gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc
    20641 aacgacacag cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt
    20701 aaacgagctg atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca
    20761 caagaagaag agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc
    20821 gtccaaataa aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct
    20881 acttctggaa cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga
    20941 acagccaaac aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg
    21001 caagcttttg aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta
    21061 gagagacaaa tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg
    21121 tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt
    21181 aagaaaaatt ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga
    21241 aactggcttt tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat
    21301 ccctttttat gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc
    21361 acaaattttt ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa
    21421 agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct
    21481 acgaaacttc cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct
    21541 agcgttctac ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca
    21601 ttaccaggca gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt
    21661 gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc
    21721 atcacggttg agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata
    21781 atcaatgaca cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt
    21841 tgttggtttc cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag
    21901 gaagtttgtg ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc
    21961 gttaaaattg ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt
    22021 gaccagattg aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa
    22081 agacttgcgg ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat
    22141 gccgggacag ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata
    22201 gctgctgcta gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct
    22261 gaaacaatag ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa
    22321 aatgcaatcc caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt
    22381 gcacacacaa caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg
    22441 accattagag ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa
    22501 ataaaaataa agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc
    22561 caatctgtcg ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag
    22621 atcgtgatat taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata
    22681 accattaata caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc
    22741 tctccttttt tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc
    22801 aattcatccg cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa
    22861 tgactccgcg gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt
    22921 caaatgtatg gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac
    22981 cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt
    23041 accttccata agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt
    23101 atttttataa ctttcacaca caagtttatt ttttactaga aagtagcgcc ctcttgtttt
    23161 tttcttgcta ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc
    23221 aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct
    23281 ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag
    23341 attgctaccg taggctggat tgttggtatg acttggctga catttcctac atcagaagaa
    23401 ccgagacttg ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca
    23461 tgctggaaga ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg
    23521 aacaatccaa actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc
    23581 actttttcat atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca
    23641 gcagcgtaat ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata
    23701 ttgacatctt ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat
    23761 gcttccaaag catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa
    23821 aatttgatgt cgactggatc attcgctaaa gattctgtcg ttttcccata acgaaaagct
    23881 ggatggacgc ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct
    23941 ttagctgatc cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca
    24001 ccagtttcat caatgaattg cttcactatg ctggcggcta atacggagta agtaccaaac
    24061 aaattatgcc cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca
    24121 attgtgggac cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct
    24181 acttcttttt ctacccaaaa cctttctttt tctaattgtt taatcaacgc tgctgaggaa
    24241 taaaactcat agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat
    24301 tctggtaaac gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata
    24361 aaaaattcct cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc
    24421 acgcttgatc cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg
    24481 tctactattt tttgatacgt tttattgttt tcatcttctt ttcttgcaac gatgatattc
    24541 acataaggac gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta
    24601 aatccagcat ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca
    24661 cgtgctgttt gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata
    24721 tcagaaacag tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc
    24781 aataacagaa gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg
    24841 tctttgatat cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca
    24901 gtatttccga tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat
    24961 tgatgttgga aagaattcaa atcaatcgat ccatctgcta aagccacatt tggttgattg
    25021 taatctgtaa atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg
    25081 ttgtcccata catctgtatc ttctccaata atccctagtt ttacgatagt ttcttttttg
    25141 tcagttgtat ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta
    25201 atgactacaa aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt
    25261 tttaatcagt tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt
    25321 agccacaatc gttacatctg tttggaatcg attataccct ctcgatatag caagattccc
    25381 taatccgcct gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt
    25441 caacgccgag acccgaatga taccaggcaa accttctaac aaatacacac gtaaaatgat
    25501 accgattgga ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag
    25561 cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa
    25621 agctgctttc gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga
    25681 aagcaagata atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa
    25741 tagcagacgg ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat
    25801 gattccaatc atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt
    25861 cgcttcgaca aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc
    25921 agtcatttta tcttcctcct tcgatcactt tgatacgact atctaaatcg tttttttgta
    25981 aaatatttat ttttacacca gatttgatca aataatccaa tgcttttttc cgctgtttgg
    26041 tttctccagc taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat aactctacat
    26101 tgccatataa aatattcgct gttacttgat atttgctgta cagatgagcg attagaggtt
    26161 cgttcgtctg atttccaata taacttaatt ccaccagcca tttattggcg atctgatcgg
    26221 caaaagcaga atgggcaatg atcgtttcta atgcttggtc aagatgagta gctgttcgaa
    26281 taaaatcttt ggtcaattct tcttcaggat tgctgaagat ctggatgctg ctaccttttt
    26341 caacgattct tccatcttcc ataaccgcta ccttgttgca gatttctttg acaacttgca
    26401 tctcatgagt gatcaggacg attgttaaac ctaactggcg attcaatttt tttaacaatg
    26461 caaggatctg aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca cataataata
    26521 ccttaggttc attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca
    26581 gttgtttagg ataagcagat gctttttctt ccaaaccgac aagttccagt agttcattca
    26641 ctttttgacg acgttcctgt ttcgattttc cagaatactt taaagaaaaa tcaacgttgt
    26701 caaaaatagt tcggctttcc attagattga aatgctgaaa gatcataccg atggcctttc
    26761 tttcttttcg caattctttg ggagagagct gactcagatt cgtctgattg acaatgactt
    26821 ctcctgctgt tggtttctgc agcaaattca tcacacgaac taacgtactt ttccctgcac
    26881 ctgagtaacc gacgattcca taaacatcgc ctttttcaat cgttaaatct acagaatcta
    26941 ctgcctgtat ctctttttct ttttgtttga aacatacaga aacatctcgc aattcaatca
    27001 ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg
    27061 aggacgcttt tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct
    27121 tcaagtacga tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg
    27181 ctcgggatga attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta
    27241 ccgaagctct ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc
    27301 aaaaaaaaag tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta
    27361 ccaccttttt tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg
    27421 ttcgttttaa cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag
    27481 gccattttca gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt
    27541 ccaaacttac tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga
    27601 ggaaaactga aaagtcaagt acgaaattca aattattttt tctatattta tttttctgtt
    27661 tttttattta acatttcaat aacagttcga agatcttttc cttccgcaag aagtttcaat
    27721 ttttcctttt cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg
    27781 cctaatcctg ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct
    27841 gaactagctt ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg
    27901 ttctccgaca cttcgtctcc gaatgtaaac gtgtccaagt aatcagctaa tttaaagtga
    27961 tccaagatat ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga
    28021 cgttctttta agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt
    28081 gtcagctgtt gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg
    28141 ctacctgctg ctttcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg
    28201 tcaacgattt cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca
    28261 cgatacgttt tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc
    28321 tatacattaa tcctttccaa aacaatacac atagtttagc acgactggtc caaaaagaac
    28381 agaagcaaag aaccagcctt ctcgtttata agagttcttg gaatcgacgg atatagattt
    28441 tcttcacagc tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt
    28501 aattgccagg aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag
    28561 aaccgacaat aattcctaat gtagtgataa caaacatggc aaatgttgcg ttgctttgga
    28621 taaatggtac tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt
    28681 cgacaaacca tcctgcatgg aataatgcga tgaaaatcac tcgctgtgaa ctatccaatg
    28741 tatggaaact tccgcccact acagcaggac aaataacaaa atacatcaac aagtaagtag
    28801 taatatcaaa tatcgaactt gttggaccaa agtaaaccat gaattcccca atactagatg
    28861 cattccattt ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa
    28921 tgcaggaaac atcatagatc agattcaaaa acaaaagttg taaaggcagc attggtaaaa
    28981 atggcaggaa cgtacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg
    29041 tagccttgat atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt
    29101 ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca
    29161 cagctgtatc tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac
    29221 catctcctaa aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt
    29281 tttgctgagg attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat
    29341 gatcactcat ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt
    29401 gacggcacac tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac
    29461 catgttcttt cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag
    29521 caagataacc aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc
    29581 ttggattggt cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta
    29641 cttttgcaag tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat
    29701 caataaattt agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt
    29761 taccagctgg atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt
    29821 catccacttt ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga
    29881 tagccacatc taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc
    29941 gcaaaacacg atcatcttca tttccttcac aatccatatg atattcaaga atgattttat
    30001 cttgtgtcaa tgtccccgtt ttatccgtac ataacacatc gattgcacca aagttctgga
    30061 tggcattcaa atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca
    30121 aattcgtcgt aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc
    30181 caaaaagaaa agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg
    30241 gagccatcaa tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa
    30301 agcttgattg aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat
    30361 ttccaacaga aacaatgatt ccttccgcac ttccactgat gacattactt cccataaaga
    30421 caagattttc gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt
    30481 ctactggata gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca
    30541 ataaacgcat atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga
    30601 cgatttcttc cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt
    30661 tcacaagtga tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca
    30721 ttgtcccgct gacgataacc atagtgaaga taatggctac accgaaaaga tctttatctc
    30781 caggagctgc taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta
    30841 atgtaaatgg cgtgatgtat gctttcaata tttctacaat gaatggtgtt tttttgccat
    30901 atgaaatcgc attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc
    30961 catttgctga tgcatggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg
    31021 catagatttt ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag
    31081 acagctgttc tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg
    31141 aaaggggctg agaacagata gataatagcc ctggcctttc ctctagatcc ttgtttttcg
    31201 aatgactgtc atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc
    31261 aatcattttt cgaatagtta tttttccaat tagaccacct cctttttatt tttcaccgat
    31321 ggggatttgc ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc
    31381 atattaaaaa aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag
    31441 cagatacctg cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc
    31501 attggcgtct ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac
    31561 aaatcttatt cggttttcct tcgttaatat aacaactaaa aaagaggatg tcaaatcaat
    31621 tttccatgtt tttcatactt tttttaaaat acacacgtta tattcctgtc tcacgcggtt
    31681 ttatttgtct cattttccac ctcccgctat aatagaaata tagtaggaaa cgaggtgctg
    31741 ttatggaaag aacacttgtt attatcaagc cagacggcgt gagacgccat ttagtcggaa
    31801 gcatcatcca acgttttgaa gcaaagggac tggccattgc tgaaatgaag tttgaaacta
    31861 tgactcctga attagcaaaa gaacattatc agcacttgac tgagcgcagt ttttttgatg
    31921 aactgatcga ttacatgact tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag
    31981 tgatcgatat cgtccgtaag atggttggag caacaaaagc tgccgatgct gttccaggta
    32041 cgatccgagg agactacgcg ctgccgggga ctgaaaacat cattcacgct tctgattccc
    32101 gtgatgctgc ggtaaaagag atcgcccgct ttttcccgcc attcgatata aacaaaccgt
    32161 ctgaaaaagc ggcgaagtga tacaaattct aaactgctgg tctagttgct gggttcagca
    32221 atggccagca gttttttctc taaggacctc cgttgattct ttcctaacat atattcgagc
    32281 actgtgaaga attcttggta gtctttcgtc ttttcttccc ctaaagcagc agaaatcaat
    32341 ctttgataat aatctgccat caatgtttca tagatcactg tacttctttc tttatgtgct
    32401 aagctccgtt tcatatcttc attcacacta cgtgcctttt tcaaccgacc attttctata
    32461 taaaacgaag tcaggttcat taaaaaagta aagagatact gctcatgatt gttcaactgt
    32521 gctaatcgtc gcattctttt tttgattttc aaataattgt tctcaatgaa ccgatcactg
    32581 aatatccaag gaaaatcagc tagaatagat agttcaaatt gaccatagtc tggtatctgc
    32641 atcaagtatt cttcaatgat tcggcattca taacgtaaaa attcaaattc tgtctcagat
    32701 acttctgttt tttcgaccat gtgaagattc aaaaccaaat aatacagatc ttctgttttc
    32761 ttaaaatgcc tacggttatt gctgatctct ttataaggat caaagctgtc ttcttgtacc
    32821 cgatctagca ttcgtctttt tttctttaac tttattggtt catccagctg gtttcgcatc
    32881 cattcatatt ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa tgttacaaat
    32941 gacatttttt tcactgaatt tgtctcaatc ccgactagag aagtcttaaa acagattcct
    33001 tccgctaatt gggactgtgt caacttcttg ttgactcgaa gtttcttgat caattcacca
    33061 tcatacataa tagttctcct ttttaattta aatttttcca cattcaataa atagatacgt
    33121 taaaaaggaa aaatacacga aaaaaataaa aaaatgtttt tttcataaaa aataccaacc
    33181 ttcaagcatt agaatgttgt ctaactactg aaggttggtt tgattgttta ttgattattt
    33241 ttttgctgtt caacgtctgt tcttgttact ggatataagt tggttgttgt tttattacca
    33301 ttttttgaaa agacacttgt cgaatgttcg cctttttgct tttcgattgc ttcattacgt
    33361 tccgcttcat ttttatagtc atattttttc ggatcaatcg tctcaaatcc gtttggcaca
    33421 taaaaccgca agagattttc ctgattcagc ttatctgaaa gacttaacgc agtcttgact
    33481 ttttcctgat ctttggagat ttcctcatcg attcctgctg ctttcgtatc gatgatttct
    33541 cctgtctgat tattgtaagc ttttcctcct aggacggtgt atttgggtgt cacaaagttc
    33601 ccatttcgaa aagcaactac ttgatcatgc tgagaagata ataagtctgt accaaaatga
    33661 agataatctt tatcatcgat tcccaataga tgtagtaatg tcggcaaaac atcgatttct
    33721 ccaccgtatt catgattgac tgtcccttta gtataaccag gcatatggat catcaatggc
    33781 actcgttgca tctgtgcgtt gtcaaattca tcccatgtat ctggatcttt accaagagcg
    33841 cttgctaaat ctttattatc tgtattcgag atcccaaaat gatccccgta aatcacaaag
    33901 attgaatttt ggtaaatacc agattttttc aaatacgtaa aaaattgttc caatgattga
    33961 tccagataat gtgccgttcg aacataatta tctactgtgc tattgccagt attcaaagat
    34021 ggaaagtcga aattcttgtc atccgtataa taaggcgtgt ggttggttac ggaaagaaat
    34081 ttcgtgtaaa acggctgttg tagatgctca agatattggg cagattctct gaataaatct
    34141 ttatctaata tcccataacc taacgtttcg tcggattcat caaaataaga tcgatcaaag
    34201 aaattgtcat aacctaagtt tttatataca tgatctctat tccaaaaact agctacattc
    34261 ccatggaaaa cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg tgcggcttga
    34321 aagacattgt ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca
    34381 gtttccaaca tattttcagc atcacttgtc tttccctgtc ctacttcatg aaagaaattg
    34441 tcaaagctga tcgttgcttg attctggaaa atactattta aaaacggtgt gacttcttgt
    34501 ccgtctacct tcatattaat caaaaactgc tggaaacttt ctaaatggag aacgatcaca
    34561 tttttccctt tagcgatccc aaatgtttcg ggatttggct ctgcataatg tttctttgta
    34621 taatctaaaa cttcatcaat cccattactg cttgcatgcg cgcgaacact gctagtcatt
    34681 cctgttttga ttccgtcata aatagtatac gcatcgattc ctagatattt gacaatatat
    34741 gaacggtcaa atgttcgctg cagcaattgt ggacggttgg cttcgctcag cgctagattt
    34801 cctgaaaaaa taagccctga aagacaggta acagcgatag ccatcggttt gccgacaggg
    34861 ttgcttttga taggtatttt cttgaccttc aaccaaacca gtagcccaat gaacacagcg
    34921 atatccagcc agtagatgac atcatggggc ttcatcaaag aaaaggagct tcctgacaat
    34981 ccttgcgaca ctttcgaaaa gcctaataca gatttgactg taatgaaatc tgtgaattcc
    35041 cgataaaaaa taatattcaa gtacaacaaa agcgtattta aaatatcaat gatcaaaaga
    35101 atagctaacg ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta
    35161 gtcgcaattg ggttgatcca caagataaag tactggatcg taccttccac acctaatgaa
    35221 aaatctgcat agtaggcaat gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg
    35281 attgctaaac gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat
    35341 tcacattttt tcttattttg ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt
    35401 aagatttttt tattctttct aaagattatt ccgtccactt ttcacagcat aaaaccgctg
    35461 cgatgtaagc gattaaacta atgaagagat aaaagtcttg tggtgaccgc ttcactccac
    35521 ggtattcaaa agccagtttc tcggtattcg tacaaaaaaa gaggctgtga caaaattctt
    35581 gtcgcagctc cttatttctt gatgcaggac agctttttca caacttcttc cttaaacggt
    35641 gttctcctgc caactcctcg gccacaagtc acaatattta taaaacatga gaagctgttt
    35701 tttgaaattg ttccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg
    35761 gtgttcaatc agctgaccct cggtattaga ttatgtcagg acgctaattt tacaacctct
    35821 tg
//
LOCUS       DACEOM010000026        35457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEOM010000026 DACEOM010000000
VERSION     DACEOM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.027"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(333..416)
                     /locus_tag="IW781_001192"
     tRNA            complement(333..416)
                     /locus_tag="IW781_001192"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(380..382),aa:Leu,seq:cag)
     gene            944..1444
                     /locus_tag="IW781_001193"
     CDS             944..1444
                     /locus_tag="IW781_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1182200.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1634..1978)
                     /locus_tag="IW781_001194"
     CDS             complement(1634..1978)
                     /locus_tag="IW781_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1182201.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2132..3052)
                     /locus_tag="IW781_001195"
     CDS             complement(2132..3052)
                     /locus_tag="IW781_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1182202.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3239..3730
                     /locus_tag="IW781_001196"
     CDS             3239..3730
                     /locus_tag="IW781_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1182203.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3754..6129)
                     /locus_tag="IW781_001197"
     CDS             complement(3754..6129)
                     /locus_tag="IW781_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1182204.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6455..7081
                     /locus_tag="IW781_001198"
     CDS             6455..7081
                     /locus_tag="IW781_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1182205.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7104..7808
                     /locus_tag="IW781_001199"
     CDS             7104..7808
                     /locus_tag="IW781_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1182206.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8000..8833
                     /locus_tag="IW781_001200"
     CDS             8000..8833
                     /locus_tag="IW781_001200"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1182207.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9030..10958
                     /locus_tag="IW781_001201"
     CDS             9030..10958
                     /locus_tag="IW781_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1182208.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11096..11551
                     /locus_tag="IW781_001202"
     CDS             11096..11551
                     /locus_tag="IW781_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1182209.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12021..12290
                     /locus_tag="IW781_001203"
     CDS             12021..12290
                     /locus_tag="IW781_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182210.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            12690..12944
                     /locus_tag="IW781_001204"
     CDS             12690..12944
                     /locus_tag="IW781_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1182211.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13033..14079)
                     /gene="holA"
                     /locus_tag="IW781_001205"
     CDS             complement(13033..14079)
                     /gene="holA"
                     /locus_tag="IW781_001205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1182212.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14117..16390)
                     /locus_tag="IW781_001206"
     CDS             complement(14117..16390)
                     /locus_tag="IW781_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1182213.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16383..16922)
                     /locus_tag="IW781_001207"
     CDS             complement(16383..16922)
                     /locus_tag="IW781_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1182214.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17058..17276)
                     /locus_tag="IW781_001208"
     CDS             complement(17058..17276)
                     /locus_tag="IW781_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1182215.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17273..17518)
                     /locus_tag="IW781_001209"
     CDS             complement(17273..17518)
                     /locus_tag="IW781_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1182216.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(17634..18362)
                     /locus_tag="IW781_001210"
     CDS             complement(17634..18362)
                     /locus_tag="IW781_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1182217.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(18521..19564)
                     /locus_tag="IW781_001211"
     CDS             complement(18521..19564)
                     /locus_tag="IW781_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1182218.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(19554..20045)
                     /gene="coaD"
                     /locus_tag="IW781_001212"
     CDS             complement(19554..20045)
                     /gene="coaD"
                     /locus_tag="IW781_001212"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1182219.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(20042..20602)
                     /gene="rsmD"
                     /locus_tag="IW781_001213"
     CDS             complement(20042..20602)
                     /gene="rsmD"
                     /locus_tag="IW781_001213"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1182220.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(20721..21059)
                     /locus_tag="IW781_001214"
     CDS             complement(20721..21059)
                     /locus_tag="IW781_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1182221.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(21043..21480)
                     /locus_tag="IW781_001215"
     CDS             complement(21043..21480)
                     /locus_tag="IW781_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1182222.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(21470..22594)
                     /locus_tag="IW781_001216"
     CDS             complement(21470..22594)
                     /locus_tag="IW781_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182223.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(22615..26043)
                     /locus_tag="IW781_001217"
     CDS             complement(22615..26043)
                     /locus_tag="IW781_001217"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1182224.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(26069..27232)
                     /locus_tag="IW781_001218"
     CDS             complement(26069..27232)
                     /locus_tag="IW781_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1182225.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(27235..27537)
                     /locus_tag="IW781_001219"
     CDS             complement(27235..27537)
                     /locus_tag="IW781_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1182226.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(27652..29424)
                     /gene="recQ"
                     /locus_tag="IW781_001220"
     CDS             complement(27652..29424)
                     /gene="recQ"
                     /locus_tag="IW781_001220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1182227.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            complement(29685..31514)
                     /gene="typA"
                     /locus_tag="IW781_001221"
     CDS             complement(29685..31514)
                     /gene="typA"
                     /locus_tag="IW781_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1182228.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            complement(31682..32449)
                     /locus_tag="IW781_001222"
     CDS             complement(31682..32449)
                     /locus_tag="IW781_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1182229.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            complement(32455..32730)
                     /locus_tag="IW781_001223"
     CDS             complement(32455..32730)
                     /locus_tag="IW781_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1182230.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            complement(32892..34298)
                     /gene="lpdA"
                     /locus_tag="IW781_001224"
     CDS             complement(32892..34298)
                     /gene="lpdA"
                     /locus_tag="IW781_001224"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1182231.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            complement(34306..>35457)
                     /locus_tag="IW781_001225"
     CDS             complement(34306..>35457)
                     /locus_tag="IW781_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182232.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
BASE COUNT        10204 a         7297 c         6173 g        11783 t
ORIGIN      
        1 tggtactaag attcattatt tgaacgttta aacacattca aaatatagta agaggtcatg
       61 cgatttattc tttcgcatga cctctttttg agtttaagtt tagtctttat tccttaacta
      121 tcgtttgcca gtatttcttg tatcagaatc tataaatcta ctatttgaat caatctatta
      181 ggttttaaac tgtcccacct tcacacaaat tttttttgat ttatttgaat ctggttccgt
      241 tgtttaaaat ttttcctata ttcttttaga taatatgtta ttttctttct catcgctcca
      301 ggtgtacctt ctctttataa aaaaacaaat tatgcggcta agaagactcg aacttccacg
      361 aggtcaccct cactagcgcc tgaagctagc gcgtctgcca attccgccat agccgcatca
      421 catcttttag tttaggatta catgtgtttt ttgtcaagaa catatttttg ttctttttca
      481 tttctgtacg ccctccctta tgccatctac acaaataatt catccgagca actccatcat
      541 ctattgaggg ttggtgaaaa aatctgtttt atccatatga tcgatttcaa tgttaaacga
      601 acgactctgg gattagtaac tattttattt ttttcttcat ttcttttttc ctctttttct
      661 cgattcagct atcttctttc atttggatat tgattatttc ttctgttgat tactatcctt
      721 ctggaattct agacgctgaa gtaattcacg ttttctttct tctagagatc ataagtaacg
      781 aatgaacgaa cttcaaataa tttttttatt gtaattcata tttgtattgt tcgatgattc
      841 ttaatacgat atgacacaat aaataaaaat aggcatggct atttacaaca atagtgctat
      901 agtagaaaac aaatgaataa ggttacttaa agaggtgaat acgatgaaac tgatcaaact
      961 atgggaagca gatttaaaga aagcttatca gctacaaact agctttggac caaatgagag
     1021 tggatttatc aacaatgctt atggctatac atttgctgaa tttttagatt atgtaaaaaa
     1081 atgcgaaaaa acgtctttag gaatcgatct tcctgagagt tacgttcccg caaccgtctt
     1141 tattttagaa agtgatgaac atgagtatgt aggaattttt aatttgcgcc ataaactgaa
     1201 cgaaacatta agaaacggcg ctggccatat tggttacggc atctccccta attatcgtaa
     1261 aaaaggatat gccacacaag gattagcatt agtactacaa gaggcaaaaa aaatcggcat
     1321 tgatgaggca cttttgtctg taaataaaaa aaatcttggc agtcttaaag cacaacaaaa
     1381 aaatggggct gcgatttatc gtgaagatga gaaagaatac tatacgaaaa tcaaactgat
     1441 gtaaaaaaga aggattccaa aaggaaaggg atttgaatgt cctttatcaa tagaacacat
     1501 ttatttaatt aacttataat atagcagaca aaattttata aacaaagaaa ccttttatct
     1561 cttttcttct agaaaggata aaaggctttt ttgtttttcc tatttgcttc tcatagctag
     1621 tcagccaaga tttctatcgt atccccaacg caatccgtgc ataacggctc atcttatctg
     1681 tcgtccatgc tggataccag actagtttga cttctgcttt cgttacttct ggcacttctt
     1741 ttaatgcgtc atggatagat tctgttaaaa tatctgctaa tggacagccc atagttgtta
     1801 gagtcatctt aatgactgtt tctccatttt caggattgaa ttctatatca taaatcaagc
     1861 ctaaattgac gatgtcgatt cctaattcag gatcaatcac catttccaag gcagcaagaa
     1921 tacgttcttt gattagttct atttcttgtc cagaccgttc ttggtttgct tcgctcatcg
     1981 tacgacactc cttaacacga tttttattta tcatactctt tttttagctg cttgtaaatg
     2041 agaatttaga agtaagattc tcgtttaaat ttataaaaat gttcttatta gatgtaatag
     2101 attaaattta ttctaaaata aacctgattc ctcatttttt ttgtgaacga ttttttattt
     2161 ctggcaatac gccagttgtt cgatacattt tttgttcgtt ttcatttgca aaccggccaa
     2221 atactgaatg agttggtaaa atattgataa atgcatcgtc gtcgatttca tgaacgatct
     2281 gatcaagatc atacatttcg tagcgggtga tgaccatcat gatcatgttc ccttgttttt
     2341 ttgaatagcc accgacagac ggcagtaatg tcattccacg taccatccgt tttgctagtt
     2401 cttctgttac ctcttctggc ttgacggtaa cgatcattgc tgttactttt tgatgactcg
     2461 tgtggatggc atcaattact tgagtcattg cataaatgga aataatcgta tacaaggcac
     2521 tttcccagtt aaagaagaag cctgcgatga ctacaatgat accatttaga atcagcatgt
     2581 agtttccgac tgtttttcca gttgtttgtg aaagtacaag agagatgata tccatcccac
     2641 cagttgtaaa gcccattttt aaagataatc ctacgccaag acctaataaa acaccgccta
     2701 ctagggcatt catcaaaata ttatcagtga tctgtccgac tggaagcagc atcgtcatca
     2761 ccgatacaca tacaacattc acaaaactaa ggatcgttgc tcgttttcct actttgagaa
     2821 accctaatac aaacacaggg atattcaaaa gaaaaataaa tgttcctgtg tctatcttga
     2881 tatgaaacca ttcatataaa agcgaggaaa tgatttgagc gatcccattc atccctgcag
     2941 aaaagacatt ggctggaatc aaaaaataat ttaaagcgac cgcagcggtc aatccggtta
     3001 tcaaaataac cactattttc tttaatcttt catttttatg atacgcagtc ataattttac
     3061 tcacaagcac ctctccccta ctttggtatt ccaaatgtaa gaataacagt ttatctatta
     3121 aaataaaata cttcgaactc aaatgagaag aattttagca aacaggtaag aatctgccct
     3181 aatcctatga taagatagat gtatggtaaa tatttatatg tatatcagga gggttcctat
     3241 ggcaaaagat gctagcttcg acattgtatc agaagtcaat acagaagaag taaaaaatgc
     3301 gattcagcaa acaacaaaag aattgtccaa tcgctttgac ttcaaggatt ctacagtaga
     3361 aatcaaatta gagaacagca atctagttgt attgagcgat gatgatttca aaatcgaaca
     3421 gatcaaagat gtattatttg gtaagctgat caaaagaaat gtgccaatca aaaatatcca
     3481 tttttccgaa tcagaacacg ctctaggtgg caaagctcga caaactgccg atttggtcaa
     3541 cggaatcgat cgtgaaaacg cgaagaaaat cactacagct atcaaaaatg aaaaactgaa
     3601 agtaaaagca caaatccaag aagatcagat tagagtgaca ggtaagaacc gtgatgatct
     3661 acaagctgtg attgctttat tacgtaaact tgacttacca atcgagttgc aattcactaa
     3721 ctatcgttaa tcaaaaaacg aggctgacgt ctgtcattcc tcgttttttt gatttactgc
     3781 ttcttttgat aaccaaaaac gctgtattcc ttcttccaat tccccaacac ttcgtacttc
     3841 tcttgctgtt tgccaatgtt gtgggaataa acgtcgataa gaaaaactag aaaatcgttg
     3901 atctgccaat acaacgattc cttgatccgc catgccacga atcactcgac caccagcttg
     3961 caagactttg ttcattccag gaagctgata agcgtaagca aatccaaggt gttccttctc
     4021 atcataatat gatttgatca gttcctgctc atgattcatc tgcggcaaac ctacacctac
     4081 gatcatactg ccgatcaatc ggtcccctct caaatcgatt ccttctgaaa aaatcccgcc
     4141 aagtacgcaa aatccaatca atgtttcagc gggatttgtt tgaaattttg ttaaaaatcg
     4201 ttcgcgttct tcctcattca tgtccgtttc ttggattagc acattgctgt cgggatattg
     4261 ctgtctgaat gcttccaccg caagatctaa atattgataa gaaggaaaga aaacgaaata
     4321 attcccgacc tttccatcag caaaagcatg aatcgtctct actaattttg ccagactgtg
     4381 ttcccttttt cgataagtcg tctctaggta atttgcgatg aaaacttgct ggtttccttc
     4441 tgggaaagga ctcggcagtt tgtaagccag acttttttgt ccccccagtg tttcctgata
     4501 ataagagagt ggtgaaaaac tagcagaaaa aagaatactg ctttttcctt tatccaacga
     4561 ctgctcaaga aacaagcttg gatcaataca aaactctttt actatcagat cgcgatacgt
     4621 tttttcgact gttgtttcgt agtgatcatc ataaaattca ctgaccttca aaaaatgaag
     4681 caaatcaaaa taataaggaa gcaattgttc ttgtgatggg tgctcaggaa attctgccag
     4741 ccattcttta attttttcgc ttaattgata acctgccttt actaaagatt cagccggtgc
     4801 tttttgatga tgataatccc aatgatcttc ttttgctatt tctcggatcg aatcaaattc
     4861 ttttaatagt ttgttgaaac ggcgatgaag ttttttgaat tcctttggta tagcttcctt
     4921 acttcgtttc gttttttcat aagatagttc tgccgaatac atctctcggg aacgattcac
     4981 caaattatgt gcttcgtcaa tcaaaaacag atagtcctcg tttttttcat cttcaaaaaa
     5041 acgtcttaaa taaacagttg gatcaaataa ataattgtaa tccccaatga ttacgtcaca
     5101 ccagatactg acatctaatg atagttcgaa aggacaaagt gtatgctttc ttgcataagt
     5161 ttcgatgact tcacgagtga tctgattctc atgattcaaa agatcccata aaccttcatt
     5221 aatccgattg taataaccat tggcatatgg gcactgatct ggattacagg tcgtctcatc
     5281 taaaaagcag atcttgtctt tcgccgtcaa agtgacactt tttacttcag ctcctgtatc
     5341 ttttaatgct gtcatagcat cttcagctac ttgtctagtt attgttttag ccgttagata
     5401 aaagatcttt tctccctctt cttcccctac agctttcaaa gcggggaata acgtagagat
     5461 tgtttttcct gttccggtag gtgcttctac aaacaatttc tgtttcgtac ggatcgtttt
     5521 ataagcagct accgctagtt cacgctgacc tttcctatac tcatcatggg ggaatttcaa
     5581 agccatcaaa gaggcattgc gtacagtgcg ccagttttct tgaaaaacaa gccattcttt
     5641 ataatcttca gtcagctttt taaaaaaagt ttctaactgc tctcttgtct gatgttcaac
     5701 ctttctcgtg atcacttctt ctgccgtctg gaagtaagtc aattgcagat ttatttcttg
     5761 taaatcatgt tcttgactat agatataggc atacaccctt gcttgatgaa agaaaagttc
     5821 cacttgttct ggttctaaat cttcaaatcg tggttcggag gttttgattt catctatata
     5881 ataaataccg tctcttttga aaataccgtc tgcacggcct tcaacgatca ggtaatattc
     5941 atctaattga actgttgttt ttaaaaagac ttctttttca tagttttctc ccgcatcttt
     6001 ttggagcttg cggtggattc tcgctccttc taatgcagta tggttacttt ttttacgatt
     6061 gtcgatactt ccacgtcgca aaataaattc tactaagtgc cgcacagata tttttgactg
     6121 tttgatcatt tccaccacct ctttctctct aagattacct gaaaaaacct agtttagcaa
     6181 aaagagctct ctttccaaac ataaaaaaac accctgctga ttaggcaggg atataggata
     6241 tagaatttgg atttttggat gaatctaatg taacaaggac atattaaaat gctatgaaaa
     6301 cttactttcc ttctctttag aatatccaaa tacttttcat atagtctgat aattcagata
     6361 agtaaagaga aataagtacc tgtttttgtt tcctagcaat gaatgcgtta tactaatgga
     6421 tgaaagaagt tttggctatc aaggaggaat gcacatgcat atcgaaagat taaaaacagg
     6481 aatgattgaa gaaaactgtt atttggtcta caacgaagaa gcacttttga tcattgaccc
     6541 tggagaagat gcggaacgaa tcaaacaaga aatcaaaaaa acaaaccaac agccggtagc
     6601 gattttgctt acccatactc actacgatca tatcggtgca gtggaacctt tacgcgcgta
     6661 ttatcaaatc ccagtttatg tcagcccact agagcaaaaa tggctaggtg atccgatttt
     6721 gaatctttct ggactcggta gacatgatga tattgctgat gttattgtct cgcctgcaga
     6781 atatgaattt gaaatgaaaa cttatacttt aggaaacatg tcttttcgcg tcgtccctac
     6841 cccagggcat tctataggaa gcgtcagctt tatttttgat gattttgtcg tttctggcga
     6901 cgccctgttt agaggaagta tcggtagaac cgatctgtat acagggaatt tagaacaatt
     6961 acttcatagt attcggaccc agctgtttgt attacctgat gaatttgcag tctatccagg
     7021 acatggcgat gcaacgacga ttgaacagga aaaaaggaca aatccttttt tcaatcgtta
     7081 aaacgactta ggaggagaat aaaatgacag aaactacact ttatatcgta cgacacggaa
     7141 aaacgatgtt caatacaatt gaacgagtac aaggctggtg tgatacaccc ctcacaaagc
     7201 aaggacaaga aggcattcat tatttaggtc gcgggttaaa aaatgtcgac ttttcatttg
     7261 cttattctag tgacagtggt cgtgcaatcg aaactgcccg tattattcta ggagaacatt
     7321 ctaagggaaa agaaattcct tattttattg aaaaaaggat tagagaatgg tgtttcgggt
     7381 ctcttgaagg tggatatgac atggaaatgt ggggtgtgat ccctcgtgta ttgaatttta
     7441 aaacatatga tgaaatgttt accacagaag tttcttttga acaaattgct aatgctgtat
     7501 acaacttaga taccgctggg tgggcggaaa cttatgaaac attgcgagac cgtgtatgga
     7561 caggatttga agatatcgcc catcactgcg aaaaaaatgg tggtggaaat gttttagtcg
     7621 tatctcatgg tttgacgatc gcctttttgc ttaatctgat cgatcctaaa cagcctgttc
     7681 gtgctgggct tttaaatggc agcgtaacaa aggtcactta tgcaaacggt cgattttcta
     7741 tccacggcat caatgatacg tcttatatcg ataacgggaa aacacatagt cgcgactcgt
     7801 ttctataatg gggatatttt aatttttacc agagaatcat aaaaggcaaa gtctgaacga
     7861 gggctttgtc ttcttttttc tcttcgtgta cttatctgct ttatttctca agtttttctt
     7921 aatattaaaa aacagattgg gtttctcaaa aaataacggt atactataag atgtatctta
     7981 attgggaagg aaggaattta tgttttttgc aaatattatc aaagcagtga ttctcggtat
     8041 tattgaagga ataacggaat ggctgcccat cagtagtact ggtcatttga tcctagcaga
     8101 tgaattcatc aaactggatg ccagttcaca atttatgtcg atgttcaatg tcgttatcca
     8161 attaggggcg atcttggctg tcgtcgttct ttattttcac aagttaaacc cattttctcc
     8221 aagtaaatca acggtagaaa aaaaagatac gtggacactt tggtttaaag tgatcgttgc
     8281 ttgcatacca gccatcatca ttggtttgcc tttggatgat tggttagaag caaatttcca
     8341 caagttctta ccagttgcca ttgttttgat cgtttacggt attgccttta ttattgtcga
     8401 aaaacgcaac aaaaacaaaa cacctcgttg gacttctctg aatgacttta cgtaccaagc
     8461 agctcttctt gttgggttat tccaagtctt ggcacttgtt cccggaacat cccgttctgg
     8521 cgcaacgatc ctaggatcta tcttgattgg tgcttcgcgt tttgtcgcta cagagttttc
     8581 atttttctta gggattcctg tcatgtttgg cgcaagcggc ttgaagatcg tcaaatattt
     8641 agctgaaggg aattcattca aaatggaaga aactgttgtt cttcttgtag gaacattcgt
     8701 atcttttgtg gtttccgtct tagctatcaa attcttacta agctatttga agaaaaacga
     8761 ctttaccgct tttggctggt atcgtattat ccttggtatc attttgattg gttattggtt
     8821 tattgcaatg tagaaaaaac cagaacactt ctcttatttg agaaatgttc tggtttttta
     8881 ttttctttaa taataatcat tataaataat aaaaacaaca tcaagcttaa tcaccaaatg
     8941 ataaccatta tcgttctcat ttagctgtta ttattatcaa ttacattttt attatgctat
     9001 ccttgttttg acaaggaggt ctaataacga tgactaatca gaaaaaagct tttgcagcaa
     9061 caatcggctg ccttttattt atgatcttcg gttttatctt cgaccgattc tctcttacca
     9121 tcaccccact tttttatatc agtgccatta tcatcggtgg atataaacaa acatcggaag
     9181 gcttgaaaga attgtggaat gatcggacac tgaatgtgga tttattgatg gctttagctg
     9241 ctattggtgc ctgtttgatc ggtaattact ttgaaggagc aatgttgaca ttcatctttt
     9301 gtttaagcgg tgcattagag gaatacacaa ctaataaaag tcaaaaagaa attactgctt
     9361 tgatgaatct tcaaccacaa aaagcccaat tattaaaaga aaatggacaa atgcaagaag
     9421 tagatgtttc tcaactaaag gtggataact tggtatttgt cgctaaagga gcagctgttc
     9481 cgatagatgg ctttttagaa agcacacagg caacgatcga tgagtctgca ttaagcggtg
     9541 aatctgtgcc tgtagaaaaa tatttaggcg atgagatgta tgcaggaacg ctgaatcaag
     9601 gaaatcccat cacgattcga gtaacaaaaa cgagttccga aactgtcttt gctaagatta
     9661 tccaattagt agaagaagcg cagaatacgc caactcaaac agcttcattt atcgaacgaa
     9721 tcgaaaataa ttatgtaaaa ttgattttgc ttgctgttcc tctaatgatc cttctccccc
     9781 actttttcct aggttggagt tgggatgaaa gcttttatcg aggaatggta ttattagttg
     9841 tggcttctcc ttgtgcgctt gtcgcatctg ctacaccagc ttctctagct gctctttcaa
     9901 attctgcaaa aaatgggatt ttaatcaaag gcggtatcca tttagaaaag ctttctgaat
     9961 taaaagcgat tgcctttgat aaaacaggaa cgctcactaa aggaaaacca gttgtaactg
    10021 acagcttttt ctttgatgaa aaggatttgg cacaacaact tcttgttgct atggaacaaa
    10081 aaacgacgca tccacttgct catgcgattt tgcaatattt cgattgcaca atacctgaag
    10141 aaatccaata cttaccaata gaagaaatta ctggttttgg attacaaact acctatttag
    10201 gtgcgcaatg gaaagttggc aagcacgcat atgatcctga gacgatgata atcagcaaag
    10261 agattgcgga aatgatcgaa cgtttggaaa atcaaggcaa aaccgtgatt tatctttcta
    10321 aagatcagca gttaattgct gttttaggtc tgctagatat tccaaaagca aatacccaac
    10381 aagtaatttc ctactttaaa tcccaaaaca tccatacaag tatgataacc ggcgaccatt
    10441 ctggcacagc aaaggccatt gctgaacaag tagggattga acattactat gcaagctgta
    10501 ctccggaaga aaaaacacaa ctagtcaaaa aagagaatga actttaccaa gtaaatgcga
    10561 tggttggtga tggagtcaat gatgcgcctg ctttagcagc cgcttcttta ggtgtcgcta
    10621 tggggcaagg gacagatgcc gctatggata tcgcagacat cgtcttaatg aaaagcgact
    10681 taaataaatt tgtctacagt catcggctat ctttaaaaat gcggcggatc atcaaacaaa
    10741 atattatttt ttcgattctt gttattctct tgttgattgc ttctaacttc ctgcaatttt
    10801 taaatctacc tcttggagtt gtaggacacg aaggaagcac aatattggtt attctgaatg
    10861 gcttgcgttt actaagacct ttacctgatc tttctgattc ttcaaaaaaa tgtaagtctt
    10921 gtccactata caaagcgcgg ctgtcttctg tcaaataagc aaagtgtttt tttatgattt
    10981 tgtttatttt atgaaaaaaa cagcttatga ataggtcttg aaggatgaat tcctactttt
    11041 gtaactttct aaaaacatgt tatgattgtt tcgaagaaag tgaggaatat atttcatgga
    11101 aagttggctt tttttaggaa ttattttact tgtagcaatt ttagctaaaa atcaaagtct
    11161 gatgatagcg gcagcttttg ttttgattat gaagctcata ccagcctccc gttccttttt
    11221 cccttggctt caaggcaaag gaatcaatat tggggtgacg atcatcacag cggctattct
    11281 aatccctatc gcaacaggag aaatcggtct acgtgatcta ttagaagcct ttaaatcccc
    11341 tattggctgg gtagctgttt tttgcggtgg gctagtggct gtcttatctt ccaagggtgt
    11401 aggattgatt gcttctgatc cacaaatcac tgttgcactt gttttcggca cgattttagg
    11461 tgttgtcttc ttaagaggaa tcgccgccgg accggtcatt gcttctggta tcacttactg
    11521 tatcttacaa gtatttgctt taggaaaata gaacaaattt tgtctatttt aggagattct
    11581 taacaccccc aatttactga tccgtgatat actgacaacg aggtgataca atgttcgaaa
    11641 aattaaagta tttcatagga acaatcggct ggtttcttct agcggtactt ttgattctgg
    11701 gcttagctta tctattattt cctgatttga tacaaaatgt gtttgatact gctttttgat
    11761 tgacctatta aaacgatata ggggatgctc ctgcgatgca ggggcttttt ttaatcaatg
    11821 ttttgtaata atttaggccg tgccaatgag acttcctata gaattcgatg ctcttttttc
    11881 tttttggctc ttctgctctt ttttgttata ctagattaca gtggacaaaa gaaagaatga
    11941 tcgcatccac ttcaacgaat atcaaaatgg aagaattagt gcctgttttc aggttacttt
    12001 gaaaagaaag gaagacgctt gtggaaactt ttgaaggtct tttaattgat acgtacgttt
    12061 tacaagaaca gccaatgatg ctgactggga tcaacttgca gcttgaggac cgtgtgatca
    12121 atggcctgct taaagatcgc tatttggcaa aatatatctt acgtttgccc ctaaataaat
    12181 atgtgctgga agtaagtggc aaatggaacc gtcggaacca gttggtcata aaaaaaatgt
    12241 tgataaaaaa tatcgatgaa tacattaaaa taatcggtac accagaatag cctttactga
    12301 tcaaaggata ctggttataa aataactgca gtttcttaat agaaaaacaa agaacaacag
    12361 ggaatcgtat cccttttcaa aaaaaacttt gcaaacggtt ctctttgtta tataataact
    12421 tagtaccaaa cattcccatt caaactttgt ttaaatagat aaataaaaat ctagtttggt
    12481 ttgacgctat tgcctcccac aatagcgtct ttttgatttt tttcagcttg tactatccgt
    12541 ttttactgaa aaaaaatcct aataaaagac tgttaaggaa aaaaacttgt catgttcata
    12601 ttgcaaagca cgctgccttt aggtataata atgaatgttg aattatgcgg taacacgcgt
    12661 aagaacttaa gttatcggag gtgaaactaa tgccaaacat tgaatctgca atcaaacgtg
    12721 ttcgtacaag cgaaaacgct aatgtgaaaa attcatctca aacaagcgct atgcgtactg
    12781 caatcaaaaa atttgaagat gctgtagctt caggtgctga caacgtagat gcgttatata
    12841 aagaagctgt taaagctatt gatatggctg aatcaaaagg tctaatccac aaaaacaaag
    12901 ctaaccgcga caaatctcgt ttaagcaaaa aaatcgctaa ataaaagaat cggcttcggc
    12961 cgattttttt atttgtcaaa aaaaggacaa aaacaaacga ccttcccgca tgctttgccc
    13021 tcgtctttct tcttatcgat ttcttaacaa ctgccctgtg cgcaaaacga atagttgaaa
    13081 acttaattct ttttcgattt gtcccgtttt gatttgataa tccatctcaa ctaattgatc
    13141 aaacaactgt accagcaaat gttcatcgaa tttttttact tcctgcatgg caagttttac
    13201 acgatatgga tggattttca atgtttcagc aatatttgcc tgctgataac caatctttac
    13261 aagaaatttt gtctgtaaaa ataaacgtaa ttgagaaagc aaaatcgcat taattttgat
    13321 tgtttcttcc ccttgcataa ctaaatcttc gtataaacgc aaagcttgtt ccgtcttccc
    13381 ttttaaaata tattgggtca tgtcaaaaat attatgttct aacgttttag gaactaattc
    13441 ttctacttct cttaccgtga tttgctgttg attttgtcca taaaggacca atttctgtaa
    13501 ctctctcatc attttggata aatctaagtc ggtcaacctc aagaataaat caattgcact
    13561 tcgttccatt ttgattgagg aattctctaa ggtattcatc agatattgac gtacttcttt
    13621 ttcattcaac tgttggacat tgatgataat tgctgttttt ttcaattgtt tcgttacttt
    13681 tttccgttca tccaactttt catatggcgc aaagaatact aatacagtcg attctaatgg
    13741 ttctttcaaa taagccgtca ataaatcagt attttgttcg attccattgt ttactttttc
    13801 acctgttaaa aaataaggat tctctacgaa tactaaccgt tgatctccga aaaacggcaa
    13861 cgtctctgct tcagctacta cctcatctaa acttgcattt tccatgtcaa aacttaaaaa
    13921 atttaagtca tcttcgccct ctaattggat ttttttgaca atttctgtcc gaacttgatc
    13981 ttggagaaac ttttctgttc ctaaaatcaa gtaacaagat gcaagttttt ctttacgtat
    14041 tttttgtaac tctgcttgta tactcaaatt actcaccact ttcaaaaatt atcctaccat
    14101 gagcagatga gaattactag tctatcattg tttccactag cccatgagga tgaaaaactg
    14161 gatgccagcg ataggtgatc attccttgtt gatccgttcg ccatgttttt atctgatttt
    14221 cttctagtgt ctccaatact tcgctgtgcg ggtgaccata acgattattt acgccgcagg
    14281 atataatccc atgttttgct tcaatatggg aaagaaaatc tgtgttactt gacgtacggc
    14341 taccgtgatg acctaatttg actacatcgg ctttcagatt aggataaatc aaaagcaatt
    14401 gtttttccat tgcttgattc aaatctccta aaaataaaaa gcgcctattg ttatattcca
    14461 tccatagacc aatcgaatct tcatttcttc cctctccctt ttcaaaagga gtcaatacat
    14521 aaagatcata ataaccgctg atcctgtccc catgtttgat ttcaatgatc tcagtaccat
    14581 ttttttgagc ttcagataaa tgcctttgta atggctcatt tcgtccagcg ccccaaccga
    14641 tataaatttt tttgatagta aaatgctgag ataacgtggg taattctccc acatgatccg
    14701 tatcgtcgtg tgtcaagatg agtttatcga ttacacttat cccctgacct tttagatatg
    14761 gaacgatatt tctttcagca ggaggacgta tcataccttg cgcccacttt tctctttcaa
    14821 agtgtaactg acctccagta tcgatcaaaa tcgtttcttt tcgaaaagga ctttgcaaaa
    14881 agatactgtc tccttgaccg acatcgataa aagcgatcgt tgaagaaaag cgtaaaaatg
    14941 gcaaagtcaa aaaaataaaa gaaaaaagac caacataaaa caagtgtttc acaggttgtt
    15001 ctttcaacct atccatacaa atcagtaatc cgaaaatata catcccagat agcacaaggg
    15061 gcaaacgtcc tataatgaga gacgaccctg aagaaaaatc gattattcgc tcaaataaga
    15121 ttaatccctc ttctatcagc aaaagtaaag tttccggcag ccagcgattg aagagtgtga
    15181 gaaataacac accaggaaga aaaagaaact tgaataccgg tgcaatcagt aatgtaaaaa
    15241 gactacctaa aataggccac tcataaaaat gccatacaat caacggcatc gtgatcatag
    15301 taaacaggac gtgagaggcc gtttttttca accaatctcc atcaaaagaa atcaggataa
    15361 acaaaaaact catgaaaaaa ctaagctgtc cacccgcatg aaaaagaaat cgaggatcaa
    15421 atagtaaaag aagccaaaag acaactgcaa ataagtccat ggtggaataa tgcttattca
    15481 gcaatttgac tagcaatcgc aataaaaaca gcaaactagc acgcaaaaca gaaatagaac
    15541 ttcctgcaat agaaacataa cagatagtta gtagactcaa cggaataata ctgacgtgta
    15601 gtggccatcc gatacgtcga aataaataat acagccaccc taaataaaat tggatgtgta
    15661 gacccgatag actgaacaga tgcagcaaac cactctccct aaaaatttct tctggttctc
    15721 tgaatcctgc atctttatag ccccataata aagaattaat atatacactt gttttttgcg
    15781 gaaattcaga ttggacaaag gagataaaag atcctctttt tctctgaagc cagtttcgcc
    15841 agtgattttg catggtgatc tgttcaatct tttgaatttg gaaccgtcca gaaattctat
    15901 ggttcctgaa gtaagcttct tggtcaaaat tataaagatt ccgctttttt gctggagaga
    15961 tgaactcgcc ttcagcagtc aattctattc cctttcctcg gaagttaagc catgttttca
    16021 attctgcttc tgtgtttact tgataagaaa gctggagctt ttgtttattc tctgataacc
    16081 ttccttccat tgtcaaaaaa ttaccatttg cacgaatcgt atctgtaaat acagtaattt
    16141 gcgcagatgt tgttttttcc tctaaaaatg aatactcttg tagcctataa gaaatggata
    16201 ccgttatgct acacaaaata cacaagtacg cgataaaacg tccttctttt agcgaaaacc
    16261 ataaaaaaat tccggcaaga agcaaacgat aaccccaatt gtcagtgtaa tacaacaaac
    16321 agataatcat cgacgtaatt gccggaaaaa tcaaccgatt tcgaacatgc ctttctaaaa
    16381 cgctagacat gttttcctgt taattcattt tgaagttttg aaaaatgctg tgagtctaaa
    16441 gcaacttggt gtacagatac acctaattgt tcgatcaacg cttgggcata aggatcattt
    16501 cgatagtcat ggagataatt gatccctttc acaccagctt gcaacaaagc ctttgtacag
    16561 gccaaacatg gaaaatgtgt gacatagatt tctgaacctt ccgtcgggat accaagtttt
    16621 gcacactgta ggagtgcatt catctctgca tggatcgttc gcaagcagtg tccatccacg
    16681 acgtaacacc cttcatctat gcagtggaca tctccactga ctgctccatt atagccgcca
    16741 gcaatgatcc ttttttctcg aacgagtgtc gctcctactt ctaaacgtgt acacgtacta
    16801 cgtaaagata acagtactgc ctgagccata aagtactggt cccaaggaat gcgttcatta
    16861 tgtgacttat cagtaacttt ttctttattc gtatcgcatg cttttgaaga tttttctaac
    16921 atgttttata ttcctccgtt ttttcaagtt gattaaaaag tactcattgc ttaaacaaaa
    16981 tattctttaa atataacgaa tcatcctgtc tgacaaaaga tcctaccctt cactgccgaa
    17041 atgaagtccg tcatccatta gcccattata aaaattcttc gaatccattt gttcaagaaa
    17101 ttccgtataa aaatcgatac ttcacaatca tggcgttttg ctacttctgt gagaacagag
    17161 atatattctt tgatcaactt gttattccga tatttttgtt ttgtctcatc gactgctggt
    17221 ggcgtgataa agaaagcatg atcagaacta tagtcttttt tcagctgaac tatcatccac
    17281 tgcagatttt ttggtacgtt tctttagaaa gttgtttatg ctctgctaga tcattcgtcc
    17341 caatgagaat aaatacttta tctgctgtat agctaagtac atctttggca aagcgttctc
    17401 gtagagcttg actgttattg ccgctgacag ccgtattcat aatatgaatc ccagatattt
    17461 tattttggag atagacattc agcattggtt tttttaatgt ttctttgcga acaatcaagc
    17521 tatttcctat ccaaaaatac tacctcctcg tcctgccaag ctcttagaat tggattattc
    17581 ttagtatcca ttagcgtcca tttattgctc gtgccagtcg attttgaatc attctattta
    17641 attgtaacga agttttttag tttttcaact gtcttttcac cgataccaga aatctcctgc
    17701 aggtcttcca ctttctggaa ggatccgttt tcttctctaa atcgaatgat ttcttgcgct
    17761 ttgactgatc cgatcccagg aatctgctga agttctgatg gagtggctgt attgatattt
    17821 attttgggag gctcggaatc tgctgtaggc tgttttcctt ccgaggatgc caactgatca
    17881 agatgttctg gcagctcttc acctacagca gggatatata ggacttgctg atcttctatc
    17941 ttcgcagaaa agttgattcg atcgctatcc gcttcttcta ataatccacc tgcacgcttt
    18001 agtacatcat agacccgttc ttctgaagaa aaggaataga taccaggctc cttcacactt
    18061 cctttgatat cggtgtacat gacctttgga cgagcatcct cttctacatc gtttccttca
    18121 gctgatggat cttctccact ggaactaaac gaagaaagta tttcttttgc agcagtctct
    18181 tctgtttctc tataaaatag caaatttgat aataaaaaac cacttgcgat acaaaagata
    18241 agaactatca tagacatcca aatgatcaat ttgggtgaag ctagtatttt cttgctgatt
    18301 ttttctatca ttgttctttt ttcaaagaat gaccagtaaa aatcattcat tactcctttc
    18361 atttaaaatt gaagaaacag tgagctctgg caaaatcgtc ccttcaacat ctaattacgt
    18421 caattttttc ttttgttttt atttttatcg tgaagatttt tgacgaaaaa gaggggttgt
    18481 gacagaactc gtgtcacaac ccctctttaa ttatttagtt tcatttcatt ttctctagat
    18541 agtcaagcgc ttcttgtact gtttttactg gtacgatttt cattgaggaa ttcaattttt
    18601 tagcggcagc ttttgcttct tcgtagttgg tttttatttc aggatattct ttcttaacat
    18661 cgtctgaaat cgtatcatct ggtgcaaaga aaatctctac gccagcttta tccgcacttg
    18721 ccactttttt atcgattcct ccgattcggc caacttctcc ttcactgttg atcgttccag
    18781 ttcctgcaat ttgcttacca tgtcgcaagt ttttaccagt gacttgttca tagatttcta
    18841 aagtgaacat caatcccgct gatggcccac cgatagagcc agaattaata cgaacatctg
    18901 tatctgattc gatctcggta tggtcagtaa gcgtgatacc tattcctgcc tttttatctg
    18961 ttggtaactc gatcagatca ccagaagctg ttttttcttc accattatgc aaataactga
    19021 tttcgattgt ttgtccaact ttttggcttt ttacataatc cataaatgct tctgtacttt
    19081 gaaagctttt cccgtctact tttgttactg tatctccaac tgaaagcttg ccaaagaaat
    19141 tcgatgtttt ctctacactc atgacatata cgcctttgaa ttccatttta taaggcttgt
    19201 ctgcaagttt taacgcttgt tcgattgctg cattttggga agaatccata taatactgtt
    19261 gcatttgatt atattcttca tttgtcgccc caccagtcaa ttcttcttca ctgatcaatt
    19321 cttcaaaagg ggtcatttta gcttttaaag ctaagaaacc tgtagcttgc ctgataccaa
    19381 cagaggtaag agagaaagac ccagaacctt tatcttcctt atcattgacc gtaattagtt
    19441 ctttcagatc gatcgtggct cccggtgatt ctacataata cggcaaggga actactacaa
    19501 atccaatgat caacattcca aatatgataa tcagtactgt tttcaaccat tgtttagctt
    19561 tcatttgatt cacgctttcc agctaaagct tcgttgatca ctggcggtaa gtagctgctc
    19621 acatctccgc caaagtgcag tacttctttt aaaatactgg aactcacgtg actatattct
    19681 ggttttgcta ataagaagac agtttccaat tcatcatata gatgatgatt catctgagca
    19741 atctctcgtt catattcaaa gtctttgata cttcgtatgc ctctgatcaa cgcatctgca
    19801 ccaatctctt ttgctgtttc taccgtcaat tgattttctt gatgcatgac ttttacatta
    19861 ggaagatgag aaacagcttt tgtgatcaat gtcattcttt cttctggggg aaacaaactt
    19921 ttctttgaag tattgataaa aacaccaatt actacctcat cgaataattt agccgcacgt
    19981 tccaccgtat ctaagtgacc ttttgtaaaa ggatcaaaac ttcctgggaa taatgcacgt
    20041 ttcattgatt tccctctttt ctatagatag ttacggccgt tatgccataa atctgttttc
    20101 ttatttgatg gaatccagcc acagtttctg gcaattcgac tgttttatcc gtttcgcata
    20161 cgataactgc ggttttagaa aaaagtccca gttcttccat tttatggatt tgatcgatta
    20221 ttttttgttt tgcatatggc ggatcaagaa acaagtagtc gaattgttgt ttctccgctg
    20281 ctagccgttc gattgctttg tccgcatcaa gttttattgt ggtaaatttc tctggttctt
    20341 tcgtaatttc gatgttttct ttgatgattt ttagcgcttg gtaatttttg tctacgcata
    20401 ccgcatgact gcagcctcta gatactgctt cgatagctaa accaccgctt cctgcaaaca
    20461 gatccaatac tgtctctcca tcaaaatatg gtccaatcat attgaatatg gattctttta
    20521 ctttatctgt agttggtctg gtgttcgccc ctgctaaact ttttaaacgg cgcccgccat
    20581 aatttcctga tattatccgc atctactcac tccttagaaa tggtactggt actagtatac
    20641 caaaaaaata cttggacaat gatcaaaaat cgatcgttat ccaagttatt ttttatttaa
    20701 aaactgctca taagagattt ttaaagatgt tcagctaatc ggatttctgt atttagctct
    20761 tcaatttcga aaccttcgtc ttttaatact gttttgtttc ctattttttc ggcaaaattc
    20821 atttcgatat ccggtcggta agaacgttct acgtttcgaa caaaatgtaa tttacggatt
    20881 cgttcttctg tttcttccaa tgtctcttca tttaagtaaa gcacgacata tttcattctt
    20941 cttgagacat aatgcaacaa gccgaacttt ttcagattct ttaattgttt taagctgtag
    21001 acccatacga tcagcccgcg tcgttttgtt atttcaaatt cactattgtc taattgcatg
    21061 acaattacct ccgcatccaa agtgtttttt cgtttcaaaa aacgggctac ctgcatctac
    21121 tttgatttca ttagaaactt cttcggcaag tacttggaca atttgatcca acagactttg
    21181 cagctgtgtt tcactatatc ggaaagctgc aacatgctca tccatatcaa gctgtctttt
    21241 tgcttttcga acagctcgtt ttttttcacg ataatcaggt gcatactcac catatgccgc
    21301 tacattttca aatgcttctt tcttagacaa gaacgcttct ttcaactggc aaacttttgc
    21361 agattcttcc attgactgtt tatgttcaag ataagaagcc atcgttttac tcttttttat
    21421 catttcagcg acttggaggc attgatcctc cagttgaaac agctcttcgt taacgatcac
    21481 tgctatcact actttctccg ttttcttctt gcaataagac taacatattt tctttagaaa
    21541 aagcaatgcc tacagaatcc gttcccgggt ccatgaaacg gtcatggttg taaggatcgc
    21601 tgtaccaata taaaaacgaa aaagtagcgt caatcgccgg atgaataaat agcccactag
    21661 tattttctaa tttttgttta ctgattaact tctccaattc atcattcgtc agtgagaatt
    21721 tcactgtcgt ttgagcactg cctagcgatt tttgccaatc agccatacgt tcctctgaca
    21781 atatttcctc aggccttgtc aaaaaatcat gtagtagcag ctgactgtgg ttttgaaagg
    21841 caagtgtttg attgtatatg ggaagttcgt cttctcttct aagcatattt aataaagtcg
    21901 tactttttcg ttctttaacg cgattgattg attcccaatc agccgattta tctgcttgat
    21961 aatgtggaag gccttcacca aacacttgat atggcaatag tttcaaaaga ctatctttat
    22021 ctaaatacac aactgaaaat aggccgtttg ttgtttgatc aaaaaataaa atagcaaaag
    22081 tgtcgttatc aaaagcaatt agcggacgat aattcatatc ctcttccatt agttcgattc
    22141 caacttctgt atcatggtac tcaaaagtga aattagtata gatagtagtg attttcgtaa
    22201 gatcttgcat cgtcatccct aaataaaacg gcgcgatatc agcaggccct gtttgcagta
    22261 ctttaacagt tgtgactctg ctgtcttgga tgttcgcttc aaatgtgaga tgactagctt
    22321 tattttcata atatcttgtt tcaaaaccaa atccgctagt taatcgatca taggcttggc
    22381 caaaatcctt ttccagctga tcgatcggtt ttccgatata agtagaaaaa ccagttgctt
    22441 taagctcctg gtatttccac gttgtcattt ggtttgatat ttgttgatca gagttttcct
    22501 tcacttcaag ttttggcgga aacaagactg gttccatgta gaatgccaca ataactaata
    22561 gcagaatcag ccaaaacaaa cccagtcttt tcatgtttcc ccacctttcg cttattattt
    22621 ctcagtcact tcaatcaaaa ggtcaccaga cgaaatagtg tctcctgaag acacatatac
    22681 atgtgcgact tctccgtcaa aacgtgcttc gatggccgtt tccattttca tcgcttcagt
    22741 aatcatcaac gtatcacctt ttttaacgtt atcgcctttt ttcactaata catccaaaac
    22801 agaaccagac atcgttgctc caatttgttc tcggtttgtc ggttcagctt tcaatcgggt
    22861 ttggaccgta ctgatgatcg aattatcttt gatagtgatt tcacgtcttt gtccattcaa
    22921 attgaagaac agtacgcgat tcccttcgat atcgggttct ccgatttcat ctaaacgaat
    22981 gataagcgtt tttccgcgtt cgatttgtac attgattgtt tcacccaaac gtataccttg
    23041 gaaaaatgtt ggtgtgtcca gtaatgtaac atcgccaaat tgattatatg ctttttgata
    23101 gtccaaaaag acttgcggat acatcaaata gcttaatact tcgtcttgtt ttggttcata
    23161 gccaattttt tctgccaatt ctttttttac ttcgttgaag tcgactgatt cagccaaaag
    23221 acctggacgt tcattcaatg caggtctgcc tttaagaata atcttttgca attttttagg
    23281 aaatccgcca acgggttgcc ctagttctcc ttggaagaaa gtcacaacag attcagggaa
    23341 acttagtgtt tcgccttttt cataaatatc ttcttctgta agatcatttt ggaccataaa
    23401 tagtgccata tctccaacta ctttagatga tggagttact ttaacgatat cgccaaacat
    23461 caagttgaca gtatgataca tttgtttgat ttcatcccag cgatgaccta atccgacagc
    23521 ttttgcctgt tgctgtagat tcgaatactg tcctcctggc atttcatgca tatatacttc
    23581 tgtttcagga gcattcaagc cgttttcaaa cggtttgtaa tacatgcgca catcttccca
    23641 ataatggttt aatttctgtg cattttcaat cgtgatttct ggcagccgtg gaccattgac
    23701 cagtgcataa tacaagctgc tcatgctagg ctgactagta ttcccactca ttgcactcat
    23761 tgccacgtca acgatgtcta ctcctgcttt tgaagcagca gaataagtga tgattccatt
    23821 cccgctggta tcatgagtat gcagatggat tggcagatct gtcgtttctt tcaattcact
    23881 aatcaaacga taagctgcct gcggtttaag cagcccagcc atatctttga ttgcaatgat
    23941 atgcgcaccc agttgttcta gttcctttgc catgtcttta taatactgaa cattgtattt
    24001 tgcccgactt ggatcgttga tatcgccagt atagcaaata gctgcctctg cgattttacc
    24061 agtatcccgt acagcttgga tacttttttc catttgtgga gtccagttca aactatcaaa
    24121 gattcgaaat acatcgattc cttgcgctgc tgcttctttg acaaattcca ccaatacatt
    24181 gtcaggataa ttagaatacc caacggcatt tgaacctctg aaaagcattt gcagcaacgt
    24241 attaggcatt aaagaacgaa tttttctcaa ccgttgccaa ggatcttcat tcaaaaagcg
    24301 gtaagccaca tcaaatgttg ctccccccca catctcactt gaaaagagtt caggaagacc
    24361 ttctccagtc aaacgtgcaa tctgcttgaa atcctttgtc cgaacacgag ttgctagcag
    24421 actttgatgg gcgtctctaa atgtcgtatc cgtcaacaac aaattttcct gtcctttgat
    24481 ccattcgacc aaagcatcgg cacccttcgc atctaataca ttcttagccg ttacgtaatc
    24541 agttcttgtg attagatctt taggcatacg tggttcttca taaaatggtt tttcgccact
    24601 ttcgatacct ggaaaaccat tcacagtaat ttcaccgata tatttcatcg ttttgtttcc
    24661 gcggtctcgt agtcgaggaa attcaaataa tgttgctgta ctatcaatga aggttgtttt
    24721 tgcatttcct gattggaatt caggatgtgt gatgacattg agcataaatg gaatgtttgt
    24781 tttcactcca cggattctaa attctttcaa gcaacgttcc attttctgaa tggctgtttc
    24841 aaaggtagca ccatgcgtac atacttttac tagcaatgaa tcaaaatacg gtgtcactac
    24901 atagcccgcg taagcatttc cgacatccag acgtacgcca aaaccaccag gtgaacgata
    24961 cgtatcgatt ttccctgtgt ctggtaaaaa gttattcaac gggtcttctg tagtgattcg
    25021 gcattgaatg gctgaaccat ttaatttaat tccttcttgc tgaggcaatc cgatttcttt
    25081 atgcagatcc aaaccttgag caatcaacag ttgcgttgtt acgatatcaa tatctgtaat
    25141 catttctgtg atcgtatgtt ccacttggac gcgagggttg acttcgataa agtaaaaatc
    25201 atctccttca acaagaaatt ctactgtacc tgcgttgaca tacccgacat atttcatcaa
    25261 ttggacagct gcctggcaga ttttttgacg ttgctgttca ttcatagaga cacaaggcgc
    25321 tacttctact actttttggt gacggcgctg aacagaacaa tctctttcaa aaagatggat
    25381 gacgtttcca tgcgtatccc cgagaatctg tacttcgata tgcttaggat tagcgatgta
    25441 tttttcaaca tacacttcat cactaccaaa ggctgctttt gcttcgcttt tcgcacgttc
    25501 gtagccctct cttgcactct tttcatcgtg agctacacgc atgccgcgtc cgccgccgcc
    25561 tagagctgcc ttgatcataa tcggataacc gtattgtttc gcaaactcca aggcatcgtc
    25621 tatagaagcg attggtccat ctgtgcctgg aatcgaagca atccccgctt cgatagctgc
    25681 agcttttgct ttgattttat ctccaaaaat atctaaatga tgcagtttgg gtccaacaaa
    25741 aatgatgcct tcttcttcgc accgttgcgc aaaattcaag ttttccgaca aaagaccgta
    25801 acccggatga atagcgtcag ctccgcattc tttcgcgata gaaatgatcc cttcaatatc
    25861 tagatacgca tcaatcggtt tttttccttg tcctactaaa tatgcttcat ccgctttgaa
    25921 acggtgtacg gaatattcat cttcttctgc atagattcca acagttttaa ttcctaattc
    25981 tgtacaagca cgaaatacac gtacagcgat ttcacccctg ttagcaacta aaactttttt
    26041 catcgaacca ctcctacatc tttttttatt ataaacctaa gctcttgcgt ttttcatccg
    26101 cactgatgtt gaggacaaag ccgacacaaa tcgaaagcat cagcaaactg gagcctcctt
    26161 gacttaaaaa tgggaacgtg atccctgtca aaggaataac gccagtaatt cctccaagat
    26221 tgacaaatac ttggatcaaa aacattgcgc cgatcccaat gcacaaaagc gaattgaacg
    26281 gatcttttga acggatacct acaaggacaa tgcgcacaat cataaagaat agcaacgcta
    26341 atatcaaaat ccccataatc actcccagtt cttccactac gattgcataa ataaaatcgg
    26401 tttgtgcttc ttgtaaaaat ccttttttct ggatactgtt acctaatccc ctgccaaata
    26461 gtcctccgtt gaacatggca tagtagccgt tgactagttg atgcccttta tttaattcgt
    26521 ctgagaaagg attttggtaa atagcaaatc gattatagat atactgtaaa cgtccgggta
    26581 agatctttcc gtttgtgata ttgatcagcc agatcgtaaa agtacttaga caaaagcctc
    26641 ctactcctac aatcaaagta tacacataat tgactccgct ggctaaaagc agaaccaaaa
    26701 taatcaaaaa gataactgcc gcattaccaa aatcgggaag aatagcgact aatgcagtaa
    26761 gtccgataac cattgcaagt ggacgtttca cagtcccaag aaaatctttt tgtacactgt
    26821 tttgtctttt agataaagtc agagacaggt accagataga aatgattttt aggtattcgg
    26881 caggttggat cgagaatcct gcaatttgca gccaaccttt cgccccattg atctccttac
    26941 caaaaaacac aacaatcagc agcaaaatcg tcaaaactgc gatggctgcc atgatcaaac
    27001 gctggttttt caaaacatct gttttcattt tataaagtag tgcgatagca atcaagctaa
    27061 gcacccaaaa aatactctgg ttaatgactg atgcggacgg attttgtccg ttttctaata
    27121 atagatacga cgtagaacta tataccatga tcaatcccaa tacacataaa attaaatagg
    27181 gaatcaagat gctatagtct aatagatgcc tttttttcac tttcttaggc aagtctattc
    27241 ctccttgcca acttcttttt tatcataaat ttcttggtaa acctcactat ataactgatt
    27301 caattctcgt tctagctcac ttaacatcag ctggccctcg ttctttccga ttactcctat
    27361 acggacagca aaaccgactt gccgggaaaa gccgtacatc tgtgtatcta ccacttcttc
    27421 gaaggctttg cattgtgaga tacagagact gttgttttga tttcggatta atcgtttgat
    27481 ccgttctgct tcatcattta atacttggat cgcaagtttt ttcgacaaag aatccatctg
    27541 tgcttacctc ccattctaat tccttaatta taacatattt gattttcata aggagcaaaa
    27601 aaaaacagat ttaaccagtt gtttttattc atttttacgt attcgttctt tctacttatc
    27661 cagaacgtta gagtccgcat tttttatccg agataaaaag gcttcgccat atttatcaag
    27721 cttgctttgg ccaactcctt ttatctgaag catctccaat ctgcttgatg gacgtattcg
    27781 gctcatttct ttcaatgtgg aatctgaaaa tacgacatac gggggaacgc ctgcttctga
    27841 tgctagatcc gttcgcagtt cccgcagcac ttcaaatagt gtatcggttt cttcatccga
    27901 tagctgctgg actttcttgg gttcctttcg ataaacagat tcttgtcctt tcaatacttg
    27961 tatccctctt tctgttactt tcaaaacagg atactcacca ttgatggtca gaagatagcc
    28021 attcgatatc aaatattcaa ttaactgcat cgtttctttt tgcgattggt ttttcatcag
    28081 tccataggta gataaatgtc caaaacctaa agcttgcagc ttttgttctt tcgatcctgc
    28141 taagactttc atcagcattt gtttaccata gttctcaccc atacgtttta agcaagaaag
    28201 aaccatctgt gcttctgtcg ttacttctac tatttctctg tcatctaaac agttaccaca
    28261 acggttgcac ggactgcttg tctcgccaaa atagtttagg atgtattgct gaagacagga
    28321 ctcaatatac acgtattctg tcatcgcttt taatttttca tattcttttt gcttttgccc
    28381 ttcttctctt tgggactgct ggataaaaaa cttttggact tggatatctt gaggagagaa
    28441 aagcaggatg gcttcactgg aaagaccatc tcttcccgcc cgaccagctt cctgatagta
    28501 tgcttctaga gagccaggaa tttgataatg gataacaaaa cggacattgc ttttatttat
    28561 acccatacca aatgcatttg tcgctacaat cagctgcaag cgatcgtata aaaaggcttc
    28621 ttgcatttct gttcgctcat tctcatttag accaccatga tatctaccca cggaaaaatc
    28681 gaatttttta agcaaatgat agagacggtc tacttctttt ctcgttgctg cataaatgat
    28741 accagattga tttttgttga tctttaaata ttcgattaga tattgttctt ttttttgatc
    28801 tttaattact tgaaatcgta gattttctcg ttcaaaacca gtctgaatat ggttgttttc
    28861 tggaattttt aaaagtcttt ttatgtcagc agctacttgc acagtcgctg tagctgtcaa
    28921 agcaacaatt acaggacgat ttggcagttg atctagctgg ttcgccatct gtaaataact
    28981 tgggcggaaa tcatgccccc actgagagat acaatgtgcc tcgtcaatag cgatcatacg
    29041 gacgttccat cgcttcattg cttggataaa atccggcatg ataaaacgtt caggagcgat
    29101 ataaagtaaa cggtactgct gtctatcgat ttcttggaag cgtctagctg tttcgtaacc
    29161 atctaacgag ctattgataa aagtggcggg tatacctaat tgattcgccg cgtccacctg
    29221 atccttcatc aaagagatta aaggggacac aaccacagta agcccatcta atagtaaagc
    29281 tggaagctgg taacagattg atttcccact tccggtaggc atgattccta atacattttc
    29341 ctgatcaatg actttctgga taatttcttt ttgaccaggt ctaaactcat catagccaaa
    29401 gtattgtttt aataagtcgt tcatcttccc gctcctttct cttgccattt tacttcgatt
    29461 ttcttcgaag gacaagttga tctcctttcg tgaaacatta tcttattatg aaacaaaaac
    29521 tctctagaaa taagtttttt aaaagtcttt taaacgtctt tcggcgtatt ttctgaattt
    29581 atgtccattg acaaagatga cttctccaca gaaaagagtt ggacaaaaaa agaaaccagc
    29641 tattttttat aacaagctgg tttctctctt catcctttta cagattattt tttcttttta
    29701 gatgcttttt cacgagcatt tttctctaaa atttgttttc tcaagcgaat gctttctggt
    29761 gttacttcgc aatattcatc atcattcaag aattccaaag attcttccaa cgtcaattgt
    29821 tttggttttt tgatgacaga tgtttggtct ttagtagcag accgtacgtt tgtcatttgt
    29881 tttgctttcg tgatgttgac agtcaaatca ttatcacggc tgttttcccc gataatcatt
    29941 ccttcatata cttctgtacc tggttctacg aagactgtac cgcgttcttc gatactcatg
    30001 atagaataag tcgttgcttt tcctgtatcg atcgaaacaa gcgccccttg gtgacgtccg
    30061 ccaatttgac caggaagcat tggcagatat tggtcaaaag tatggttcat gattccgtaa
    30121 ccacgtgtca ttgaaaggaa ttcagttgaa taaccaatca aaccgcgggc aggtgttaag
    30181 aatgtcaaac ggatttgtcc gtttcctgta tggaccatat cttgcatttc acctttacgt
    30241 aagctcaagg actcgataac actacccata tattcttctg gtgtatcgat ttgcacgcgt
    30301 tcaaacggtt cgcattttac accgtcgatt tctttttcaa taacttctgg acgagatact
    30361 tgcaattcgt atccctcacg gcgcatgttt tcaatcaaga tagacaaatg caattctcca
    30421 cgaccagaaa ctgtccatgc atctggagag tttgttggtt caactcgtaa ggatacatct
    30481 gtttgaagtt ctgccatcaa acgttcttca atcttacgag cggttacgaa tttaccttct
    30541 cgaccggcaa atggtgaatt attaactaag aaagtcattt gcaatgttgg ttcgtcaatg
    30601 tgaaggatag gtaacgcatc ttgatgatcc acaggagtaa ctgtttcacc gacaaagata
    30661 tcttccatac ctgagacagc aatcaagtct ccagcttttg cttcttgaat ctctagacgt
    30721 tttagtccaa agaaaccaaa taatttcgtt acacggaatt tcttaactgt tccgtctaat
    30781 ttaattaatg ctacttggtc tcctactttg attgttcctc taaatacacg tccgattccg
    30841 atacgaccta cataatcgtt gtagtcaagt aatgaaactt ggaattgtaa tggttcgtcg
    30901 ctgttatcga caggagcagg gattttttca ataattgtat caaaaatagg tgccatggtt
    30961 ggttcttgat ctgctggatc atctgacaag cttgacgtac catttagtgc agatgcataa
    31021 atcacaggaa agtctaactg atcatcatct gctcctaatt cgatgaatag ttcaagaact
    31081 tcatctacga catgctcagg acgagcagaa ggtttgtcaa tcttgtttac gacaacgatt
    31141 ggtgtgatat gttgttccag tgcttttttc aatacaaaac gtgtttgagg cattgttcct
    31201 tcatacgcat ctacgaccaa cacaacgccg tcaaccattt tcatgatccg ttctacttcg
    31261 ccaccgaagt ccgcgtgtcc tggagtatcc atgatgttta cgcgaatacc tttatagtct
    31321 acagctgtgt ttttggctaa aatcgtgata ccacgttctt tttctagcgc atttgagtcc
    31381 atcgcacgtt cttgcaattg cgtatgtgcg tctaatgtat ctgattgttt taatagttca
    31441 tcaactaatg ttgtttttcc gtggtcaacg tgggcaataa ttgcgacgtt acggatatcg
    31501 tttctataat tcaatgtaac taactccttt tttattggtt ctttatgaca gggcaaattt
    31561 tactcgactg ctaattatag cagacttttt tcatgaaata tagtaaaatg ttttcatttt
    31621 ttcgagaaaa ttaatctttt gctcgttttt atcatttttg tgaatgattt ctaccttttt
    31681 ttcagccttc ttttttcaaa aggtctgcga tttctgcata tgcacaagaa gtccctgcta
    31741 aatagtattc ccttttttca aatgtcaaag gagtaccaaa aagaccgctt gttaccatac
    31801 caaactcttc caataagaca tttcccgctg cataatccca agggctaagg tttgaaatat
    31861 aagcaatgtg gtttcctttc aagattgcca ctagttcaag accagcgcat ccgtacatac
    31921 gtgtccccat gctgctttcc gcaatttctc ggattccata tttattatga ccgaaaagat
    31981 aactattcac accaagtaag ccttccgata aggatttatt tgctggtcta ggcaatggct
    32041 catcattttt atacacgcct attccttttc caccccaaaa aagttcattt ctcatcacgt
    32101 cataaatgaa accaagcttt ccaacgccgg attcataaac agcgagcata atacaaaaat
    32161 tttccccttc aatcacaaaa ttcaaggtgc catcaatagg atcgatgata aagactcttc
    32221 catcgagatc cgataattga ttacaccctt cttcttctgc caatattttc gcctctggat
    32281 caaaggaatg gattttttcg attagaaaat tctggatgtt tcgatcgaca tttgttacta
    32341 gatccgttcg atttgatttt tgcgagattg ttaatgtttg ttgagaagat ttgattttct
    32401 caccagcttc ttcaagccag gacttgatat gatcaatcat ctgttccatt tatctcaccc
    32461 gttcattctc aaagttttgt ttggattttt ttttgcttgc tgcactgtac gatataaaga
    32521 ataaccggaa gctttttcaa attcccgtcc caatcgtttt tcctcaccaa tggatttgac
    32581 aacagtttta aactgctgat aagtcttcaa aaaagcttca ttgtttatcc ctttttcatt
    32641 cgcacgttcc agatcttccc acatcttcat tacaatgacc atttcgtctg ttgtccaatc
    32701 taaatcaagt ggatactgat attcttgcat cttcaactct cctatttcat attcctcttc
    32761 agtgtagcat aggaagtggt aataaaccaa gaagttgtca tatttttaag tttacataat
    32821 aatattattg ataattctaa aaaacaaaac gcaactgatt tttcagttgc gtttcgcctg
    32881 tattattcaa atcaaatatg gattggtaaa cctaatgcta attccgaagc atccatgacg
    32941 atttcgccaa gagatggatg aggatgaatc gtcaaggcga tatcttcagc attcattcct
    33001 gattcgattg ctaaagctaa ttcagagacc atatcacttg cgccgactcc accgatttgc
    33061 gcgccaataa gcacgttatc ttcgatcgtc gtaactagac ggatgaatcc ttctgtttta
    33121 ccaagcgata atgcccgacc gttccctgaa aatgggaatt tatatgcttt tgcttcgata
    33181 ccagcatctt ttgcttcttt gatcgtcata cctacagaag ccaattcagg atcagtgaag
    33241 gctactgccg gcattgcttt ataatctaca gctacttttt tgccagagat tgcttcagca
    33301 gcgatttttg cttcataact ggctttgtgt gctaatgccg cccctggaac gatatcaccg
    33361 atggcataga tgttaggtac gttcgtacgt ccttgtttat ccaccgtgat caatcctcgt
    33421 tctcctactt cgacacccgc ttgttctaag cccatatcat ctgtattcgg acgacgacct
    33481 acagtgacca tcacataatc agcagtaacg gattcttctt tcccgtccac tgcatatttc
    33541 actgtcacgc tgtcaccgtt atcgactgct tctttcgcca ttgcattcgt tacgactgtt
    33601 acgccttttt tcttgaaatc atcttctact aattttacta gatctttttc gtaagttggt
    33661 aaaatttgtg gtgagccttc aagaatcgtt acttctgctc ctaagtttgc gtacgctccg
    33721 cccaattcgg caccaatcac accgccaccg atgatcacaa atttcttagg tacttctttc
    33781 aatgctaatc cgccagtaga atctaacaca cgtcctccga atttaaatcc tggaatctcg
    33841 attggacggg aacctgttgc gatgattgcg ttattgaacg aataagtttg agctgaatct
    33901 ggatgaatga cgcgtagtgt gtgatcatct acgaagaacg cttctccttc gattgtttca
    33961 accttatgtt tttttaatag gaaaccaaca ccagaagtca acgtatggac aactttgttt
    34021 tctttccatt cttgtgtttt tgcaaaatct agtttgacgt tttcactagt tactccaaac
    34081 atttcagaat ctagtgactc ttgataatgg tgtcctgcag ctatcagtgc ttttgaagga
    34141 atacagccga cattcaaaca aacaccgcca atatactctc tttcaatgat tgctactttt
    34201 tgtcccattt cagcagcgcg gatggcagct acatagccac caggtcccgc accaattaca
    34261 actgtatcta attctacagc aaaatctcca actaccattt gtttatcatc cttccatcat
    34321 taatagctct ggatcagcta aaagacgttt gatattattc attgcttgtt gcgcagttgc
    34381 tccatctacg atacggtgat cgaaacttaa tgacagtttc atcacacgac cgacaacgat
    34441 ttcgccttca gcatttacga ttggttgttg tgcaatcgtt cctacaccta aaatagctac
    34501 ttctggatag ttgatgactg gtgtaaacca accgccacct acagatccga tattgctgat
    34561 cgtaattgtt ccattgcgca tatcttctgc agatagtttg ccgtcatgag ctaattttgc
    34621 tttctcattg atttcgtctg caatagcaaa cattcctttg cggtctgcat ctttcacatt
    34681 tggtacatat agtccgtgat ccgtatcagt tgcaataccg atattgtagt aatgtttata
    34741 aacgatttct tggtttgcat cgtcgataga tgcattcaat actgggtatt tcttaacagt
    34801 cgctgttaaa gctttcacga catatggcaa gaaagtaagt ttcgtaccat ttgcagcagc
    34861 aacttcttta aatcgtttcc gattatccca tagtttagac acttctactt catcatgcaa
    34921 tgtcacgtgt ggagctgtat gcttactgtt gaccattgct ttagcgatag ctttacgtgt
    34981 tggcgtcatt gctacacgtt cttccaagtc tcctaagttg gacttgaatg gttttgctgg
    35041 tgcagctggt ttgctttctg ctgcaggagc tgcttctttc ggtgctgccg cttctggcgc
    35101 ttctgatttc gctggtgcac tgcttggacc acctgccaag aagttttcga tgtcctcttt
    35161 tgttactctg ccaccttttc cagttgcagt tacttgtgag atatctacat ctttttcacg
    35221 ggcaaattga cgaacagatg gcatagccaa tacacgttta tttggatcgg cagcttcaac
    35281 gacacttgct gaacctgatg tttccacttt ttcttttggt gcttcagctg atggcgcact
    35341 agttgacggt gcactattgt gtccaggagc atcgatttct actaatacat ccccaacatt
    35401 tgctacagtg ccttctggaa caatcacgtt tttaactgtc cctgtgacag gtgacgg
//
LOCUS       DACEOM010000027        35033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEOM010000027 DACEOM010000000
VERSION     DACEOM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.028"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..818)
                     /gene="yidA"
                     /locus_tag="IW781_001226"
     CDS             complement(9..818)
                     /gene="yidA"
                     /locus_tag="IW781_001226"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1182233.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(837..2204)
                     /locus_tag="IW781_001227"
     CDS             complement(837..2204)
                     /locus_tag="IW781_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1182234.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(2188..3018)
                     /locus_tag="IW781_001228"
     CDS             complement(2188..3018)
                     /locus_tag="IW781_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1182235.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            3181..3618
                     /locus_tag="IW781_001229"
     CDS             3181..3618
                     /locus_tag="IW781_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1182236.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            3694..4314
                     /locus_tag="IW781_001230"
     CDS             3694..4314
                     /locus_tag="IW781_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1182237.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            4503..5009
                     /locus_tag="IW781_001231"
     CDS             4503..5009
                     /locus_tag="IW781_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1182238.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            5026..5925
                     /locus_tag="IW781_001232"
     CDS             5026..5925
                     /locus_tag="IW781_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1182239.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            6012..6896
                     /locus_tag="IW781_001233"
     CDS             6012..6896
                     /locus_tag="IW781_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1182240.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(7088..7159)
                     /locus_tag="IW781_001234"
     tRNA            complement(7088..7159)
                     /locus_tag="IW781_001234"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7124..7126),aa:Arg,seq:ccg)
     gene            7219..8553
                     /gene="rpoN"
                     /locus_tag="IW781_001235"
     CDS             7219..8553
                     /gene="rpoN"
                     /locus_tag="IW781_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1182241.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8756..9793
                     /locus_tag="IW781_001236"
     CDS             8756..9793
                     /locus_tag="IW781_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1182242.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            9834..10835
                     /gene="gap"
                     /locus_tag="IW781_001237"
     CDS             9834..10835
                     /gene="gap"
                     /locus_tag="IW781_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1182243.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            10945..12135
                     /locus_tag="IW781_001238"
     CDS             10945..12135
                     /locus_tag="IW781_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1182244.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            12239..12994
                     /locus_tag="IW781_001239"
     CDS             12239..12994
                     /locus_tag="IW781_001239"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1182245.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            13110..14408
                     /gene="eno"
                     /locus_tag="IW781_001240"
     CDS             13110..14408
                     /gene="eno"
                     /locus_tag="IW781_001240"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1182246.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            14808..15947
                     /locus_tag="IW781_001241"
     CDS             14808..15947
                     /locus_tag="IW781_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1182247.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(16014..16589)
                     /locus_tag="IW781_001242"
     CDS             complement(16014..16589)
                     /locus_tag="IW781_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1182248.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(16594..17568)
                     /locus_tag="IW781_001243"
     CDS             complement(16594..17568)
                     /locus_tag="IW781_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1182249.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(17592..17636)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            17851..18819
                     /locus_tag="IW781_001244"
     CDS             17851..18819
                     /locus_tag="IW781_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1182250.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            18932..19210
                     /locus_tag="IW781_001245"
     CDS             18932..19210
                     /locus_tag="IW781_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1182251.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            19328..19564
                     /gene="secG"
                     /locus_tag="IW781_001246"
     CDS             19328..19564
                     /gene="secG"
                     /locus_tag="IW781_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1182252.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            19676..20422
                     /locus_tag="IW781_001247"
     CDS             19676..20422
                     /locus_tag="IW781_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1182253.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            20425..22782
                     /gene="rnr"
                     /locus_tag="IW781_001248"
     CDS             20425..22782
                     /gene="rnr"
                     /locus_tag="IW781_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1182254.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            22804..23268
                     /gene="smpB"
                     /locus_tag="IW781_001249"
     CDS             22804..23268
                     /gene="smpB"
                     /locus_tag="IW781_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1182255.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            23570..24289
                     /gene="atpB"
                     /locus_tag="IW781_001250"
     CDS             23570..24289
                     /gene="atpB"
                     /locus_tag="IW781_001250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1182256.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            24342..24557
                     /gene="atpE"
                     /locus_tag="IW781_001251"
     CDS             24342..24557
                     /gene="atpE"
                     /locus_tag="IW781_001251"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1182257.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            24648..25172
                     /gene="atpF"
                     /locus_tag="IW781_001252"
     CDS             24648..25172
                     /gene="atpF"
                     /locus_tag="IW781_001252"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1182258.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            25159..25701
                     /locus_tag="IW781_001253"
     CDS             25159..25701
                     /locus_tag="IW781_001253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1182259.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            25724..27280
                     /locus_tag="IW781_001254"
     CDS             25724..27280
                     /locus_tag="IW781_001254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1182260.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            27294..28196
                     /locus_tag="IW781_001255"
     CDS             27294..28196
                     /locus_tag="IW781_001255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1182261.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            28219..29625
                     /gene="atpD"
                     /locus_tag="IW781_001256"
     CDS             28219..29625
                     /gene="atpD"
                     /locus_tag="IW781_001256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1182262.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            29639..30061
                     /locus_tag="IW781_001257"
     CDS             29639..30061
                     /locus_tag="IW781_001257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1182263.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            30530..30760
                     /locus_tag="IW781_001258"
     CDS             30530..30760
                     /locus_tag="IW781_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1182264.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            30857..32158
                     /gene="murA"
                     /locus_tag="IW781_001259"
     CDS             30857..32158
                     /gene="murA"
                     /locus_tag="IW781_001259"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1182265.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            32171..32347
                     /locus_tag="IW781_001260"
     CDS             32171..32347
                     /locus_tag="IW781_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1182266.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            32482..32778
                     /gene="yidD"
                     /locus_tag="IW781_001261"
     CDS             32482..32778
                     /gene="yidD"
                     /locus_tag="IW781_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1182267.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            32962..33429
                     /locus_tag="IW781_001262"
     CDS             32962..33429
                     /locus_tag="IW781_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1182268.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            33514..34398
                     /locus_tag="IW781_001263"
     CDS             33514..34398
                     /locus_tag="IW781_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1182269.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            34515..34748
                     /locus_tag="IW781_001264"
     CDS             34515..34748
                     /locus_tag="IW781_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1182270.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            34836..34916
                     /locus_tag="IW781_001265"
     tRNA            34836..34916
                     /locus_tag="IW781_001265"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34870..34872,aa:Tyr,seq:gta)
     gene            34923..34994
                     /locus_tag="IW781_001266"
     tRNA            34923..34994
                     /locus_tag="IW781_001266"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34955..34957,aa:Gln,seq:ttg)
BASE COUNT        11704 a         6247 c         7462 g         9620 t
ORIGIN      
        1 tttatttatt acagtacata ttttttgatt gcttcagcta caccgtgctc gtcgtttgat
       61 gaagtgatga catttgctgc ttctttgaca agagaaacgg catttcccat tgctactcct
      121 agaccagcat attcaatcat ggataagtcg ttctcgttat ctccgatagc catgatttca
      181 tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg ccgcaccttt acttgcttct
      241 ttatttagta cttcgaagta atagggcgca cttttgaccg ttgtgtattt atcacggaaa
      301 gtttgcggta aacgcgcaat tgctgcatct aaaatttctg gttcgtcgat catcatcatt
      361 ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc gatattttag cggcatcttg
      421 actaaacttg cttcgtggat cgtataagcg ctgatatctc gattaggcgt gtagatcgct
      481 ttgtcatcga tcgaatggag atgactgccg acacggcgtg ccatcacttc gatctctagg
      541 aaatcttcat gtgtcagtcc gtgacgggcg atgattttac ctgatttcgt ttgttgtacg
      601 agtgccccgt tataagtaat aacgtaatca ttatcttgaa aaaggttcaa ctcgtttaac
      661 tgttcctgga cacctggtaa gggacggcca gtgcataaaa cgatcgaaac accttttgca
      721 acagcagcgg caatcgcttc ttttacttct tttgttactt ctcttttctc atttaataaa
      781 gtaccatcga tgtcgatagc gactagtttg atagacataa tttcctccaa tatattttaa
      841 tttatttcaa tcaatgcgcc attacggata tggctggcaa attcttgata agtttcgtca
      901 aataaatcat aatgatcacg caaggacgga tcaaccattt cttttggaaa gaaaaatcgc
      961 tcatctcctt gttcttgtcc cgctaatgca gcaacaagct gactaacctg tgataattct
     1021 atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc gatgtctgcc ttgttctggg
     1081 cgataaaaat cataaggtag atcgtagcta gaattcacag cagtgtaata aacaggatta
     1141 tatccgacct tttctactaa caaagtgagt tcttggacta attttgaatc ccggttgcca
     1201 gaaaaagtag cagatttcag tggtttacga ttcaaaaaac gtttagctaa atcatttaaa
     1261 ataggatcag cagattcaga ccattgagta aagtaagtgc ccaagacccc atcatcaagt
     1321 ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg gcagaagtaa ttgagaatgg
     1381 agttcaaaac tgctggcatc ttcttgatac aactcgtgtg cgcgatgaag cagatgctcc
     1441 aaaattactt ccataccacg ggaaacagga tggaagtaca cttgcacata catttgatat
     1501 cggctaacga tataatcttc cactgcatgc atgccactca tggaaaaagc gatcccgcct
     1561 tcataaggac gaatcacacg taatattcgg gtaagatcaa atgttccata ttctgttcca
     1621 gtgaaataag catcccgaag caaatagtcc attcgatctg catcgatctg actagaaatc
     1681 atttggacaa cttgtgggtt ggggtaagtt ttttggatca cactagctac cttttcagga
     1741 aaaccttctt ctactcggtt aagaatctga tacacttctg tttctggaga agtgatgatc
     1801 tctacagtga tggcttcatg attcgtatgg aagatatgtt caaatgtatg agagtaagga
     1861 ccatgaccga catcatgcaa cagtgccgca cataaagtca ccagccgttc ctgatcatcc
     1921 cagcctccgt tgcctatttt ttcttttgaa aaatttcgag aaaagatatc gcagattcgt
     1981 cttgaaatct cataaacgcc taatgaatga gtaaaacggc tatgttctgc tccgtgaaaa
     2041 gtgaaagaag aagtgcctaa ttgtttaatg cgtcgtagac gttggacttc tttcgaattg
     2101 atcaaatcta agattacttg atgctggaca tggatatagt tatggactgg atcacgaaaa
     2161 actttttcca taggcagcat ttgatattta taaggggttg tcataatgta gctcctttat
     2221 gttattacgt tgttccattc tctggatttg gttgttccat tgctccagct ggatttcttt
     2281 ttttagccag tcggtagaat cctgtaaatc acttgcagaa tacagctttt caaaagcttt
     2341 tgcaaatgct tctttgactt cttttacaaa aaggggttgg tcaagcagtt cttcaagtgt
     2401 tgccattgta gcaggatcca ctgcaggata gccatttgta ccaaagtctt cttttaaagc
     2461 agattgataa aatcgcttca tgacttgccc gcgtttttcc tgattaccgt tgacacttaa
     2521 gtacatcata acagaaatcc catttttgat ccgacgctga gcgataccgg caaatttttt
     2581 gccatggatg cttaaatcat aagttccagg acaataagaa tgggtgattt cgaatgcttg
     2641 tatcggtgcc tttggaaaag cgtatttagt caataggagc atttgctcgt aagcgtcatc
     2701 tatggttatt ttttttgtgt cactttgggg gaaaatcaaa gaaatattca aaattccttc
     2761 atctgatacg atccctaatc cacctgaatt tctgatcact ggagcgtaac ctgcatcttt
     2821 cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca tccttcatac ctaagatgac
     2881 tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg tgttctttag aatattccgt
     2941 taatatatct gttaatgcaa aaggggaaaa gtcgtttttt tgataaatgt gctgatctag
     3001 tcgaatcgca gggttcattc aaccggctcc tttgtataaa ttcatatttt ctacaatatt
     3061 atactctaaa atcaaagagt tgttttggaa aaaaggtttt ctgtaaaaat ggacaagttg
     3121 gttgacaaaa caaaagaaac taggaacaat gtagctatga gaagtaaagg agaatgaata
     3181 atggaattat cacaaggaac accgatttca atcaaattgc gtacaaaagt aaaacagcaa
     3241 ggggaagtgc aagattttta ttttgatttg aaagggcaaa tggtgacaat tggcgatacg
     3301 ctgtacatac gttataaaga gattcaggag gagacgggcg atgagattcc tgtaacgatc
     3361 aaattagttc cagatggtca tgtacagctg atccgtgcag gagaaatgcg gatgcgtttg
     3421 aaatttggct ataaagaacg gttagaaaca tcctatcgaa caccatacgg tatgatccag
     3481 attgctactt ttacgaaaga actgcatgtt agtttgaaag accgtcccac aggagggaag
     3541 gttcgaatcg attatgatct gttcatggga ccggaaaaaa tcggcgaata ttatttgacc
     3601 ttggatttta cagcgtaaat ataaaagaaa agtttgattt tttgtgtctg attttgtatg
     3661 ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa ttagtgtatt tgaaggtgca
     3721 aacaaaagcg aattatcaat gatcgaagta gcacacgcga tcttagaaca acgtggcgat
     3781 gtcatggatt tttctgattt agctaaccag atccaaaact atcttgggaa atcagacagt
     3841 gaaattcgtg attcactcgc acaattttat actgacttga atattgacgg cagctttatc
     3901 tcattagggg ataatcgttg gggcttgcgt tcatggtatg ctatcgattc gatcgatgaa
     3961 gaagtaaacc atggaatgga cgaagaggat gaagatactc cacgtcgccg taaacgcaag
     4021 aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg attacaatga cgatgaccca
     4081 gaagatacag atctgacaga agaagatgac gatgatctgt ttgatgacga tgatgatgaa
     4141 gatgaagaaa tcgctgctta taattctgac ttgcaagaaa tcggagcaga tgacccgaca
     4201 gatgatgaag aagacttgcc aggaaatatc gaagaagatt taagtatcat agacgaagac
     4261 gatgatgatg aagattatga agaggaagaa ttttctgact ctgaaaaaga ataaacatta
     4321 ttttcttacg agaggttgtg acagagacga tcagcctatc agaataagga ggtgtgacac
     4381 gatttttgtc acaccttctt ttttgtatat aaaaatccca agtaaataca taaaaaataa
     4441 aaaaattgaa acttttagcg caccttgtac gtcatattaa gtgaggcaga aaggagtgga
     4501 gaatgatttt tactggatat aagaaaaaac ggcagatcga aaaataccgt cacctgctga
     4561 atggactcat cgaaactgat tatcaaaaaa tgttccgtat cacgatgaat tacgtacatg
     4621 ataaagaaga ggccttagat gtgatgcaag atagttttca taaagccctg aactattttg
     4681 aaaaagaaaa agagatcgag cattttagtg catggtttta tcggatattg atccgcacgg
     4741 cattagatag ttggcgcaga caaaaaagga cgcctgtgga cttatttgaa attcaagata
     4801 tcccccaaca gttaccttta gaaacttccg ttgcagagct ccatatgatt ttagataaaa
     4861 tagacagtcc ggaaaaagag attctgatcc tctacttttt tgaaggattt cgtttgaaag
     4921 aaattgcgac tatcttggat atgaatgaaa acacagttaa aacgaaaatg taccgctcat
     4981 tgaatgcttt acgtcaaatc ctagaatgaa ggagagaaga gtgccatgta ttcccaaaaa
     5041 gaaaatttaa aaaaactaaa gaaaacttat aaagaacaag caattcccaa agggcttaaa
     5101 gaggagatcc gccagcgttt tattatcgaa gagcaatcat ttataaaagc aaagcggaga
     5161 aagagacgtc tccaagggtt cggagtatct ttggctatcg taatgattac aacaagtagc
     5221 cttcttttta atagtcaagt tcgaagcttt gcagaagatt tacccatctt aggatcagtc
     5281 attgaattga tcttaggcga acgctttact gatcgatctg aaaaaatcga tattcaggta
     5341 ccgaagatca atacacaaaa tgagaaagaa aataaaacaa ttcatggatt aaatcaaaaa
     5401 tactttcgcg aaggacaagc agattttgaa aaagctgaaa aagaatatgg aaattttgaa
     5461 acggatcatt accaagtttt gggagattat cagaaaatcg tagacgataa ccggtttctc
     5521 gtgattgaaa ggcagatcac ccaaacagca gcagatagcc atgttgaaaa aagatacgac
     5581 acaatcgaca agaaaaacag tgtccagctc tcacttccac ttttattcaa agatgacacc
     5641 tatctatctg ttttgacaaa agaagtcaaa cggcagatgg ccgaacaagt caaggaagat
     5701 ccatctaagt actattggac tgagcaagat attcaagaag ggacgataga aaaaccaaca
     5761 cttgttacgc ctacacgaag tttttatttg aacaaagagc atcaattagt tttgactttt
     5821 tctcaatacg aaattgctcc tggttatatg ggtacaccag aatttgtcat tcctaagtcc
     5881 gtgacaaaaa cgattttggc atcggaagat tatttggatc attagacgag aagtagttgc
     5941 gactagcagt ttcaattttc aagtaagtac aagtaaataa taagaagaga aaaagagaaa
     6001 gaagagatat agtgaaaaga aattatttag ttatcagtat tattgcttta tccgtgcttt
     6061 tgtttttcga aattacttcg gtggcctcct tgatcaccga agtaaaagga caaacacact
     6121 cttcggagca ttcgtcacaa tctttcaaac aatcagaaca atcaaccatt aaaagccaat
     6181 caactgaaac agctaacgtc aaagaagcca ttcaagaatc agcggaaaag gtacaaagtc
     6241 agtcatcaca atcaacacaa gacagtacaa aaacgagccg agatccggct gtcaaaagcg
     6301 tcgcaatcag ctttgatgat ggaccaggag cgaccacaac accgcagctt ctgcgaatct
     6361 taaaagaaaa aaacgtgcat gccacttttt tcgtcttagg agaaaatacg gcgcagcatc
     6421 ctgaaatcgt gaagcagaca gctgaagcag ggcacgagat tggcaatcac acatacgatc
     6481 atcagaatct ggccactctt tctgcccagt cgatgacgga ggaagtgacg aaagcagaca
     6541 ctgagatcaa aaaagtcact ggcaaaaccc ccacttttgt tcgtccgccc tatggttctg
     6601 tcacgaatgt aggagcaacg atcatccagc gaccgatcat cgaatggtct gttgattcag
     6661 aagattggaa gacaagaaat cctgatttga ttttgcagaa gatccaagca acagtatacg
     6721 atggggcaat tattttattc catgatatct atcctgaaac gattcgtgcc gttcctcaag
     6781 tgatcgatta tctgaaagag caagggtatc gcatcacgac agttggggat ttattagggc
     6841 atccaacggc tgtcgagaac tattatggca gaaacgatca tcggccagtt cagtaagtga
     6901 gacaagtatc tttaataaat aacacatttt aataagtagt tgaacacata actaccttac
     6961 ttgcaaaggc ttaacaatat ctatacaggt acaacaaaaa actgtccacc aattttcttt
     7021 ggcggacagt tttcacttaa aaataaagaa gcaactattc gtaaattatg ctaaaatagc
     7081 agtagaacgc gtcctgaggg aatcgaaccc ccgtctcaag aaccggaatc ttacgtgata
     7141 tccactacac taaggacgca agcactggga taattgtaac taaacagata aaaaattaca
     7201 aggggattgg acaaaaaaat gaaatttgag caaaattttt cacaaaaaca gcagcagaca
     7261 cagaaactag ccatgacgca acagttgcaa cagtccatcc aagttttaca atataacagt
     7321 gaggagctgt tggcattcgt tgagaatcaa gcaatggaaa atccattggt ggaagttgtt
     7381 gagccggaat ggcagccaga ttacataaaa gcttcttcat cttcttatga aggagaagag
     7441 acaaattacc taaatcagat tcctgataca aaagggtctt tatttgattc attgatcgaa
     7501 caagtccatt tgaattaccg agatactttt ttgaggaaaa tcgttcttta tcttgtagaa
     7561 tacatcgatt tgaacggatt tttgacgatt agtttagaag aagccggaaa agagattgga
     7621 gctacgccta tccaaatgct cgatgcgttg actctgattc agcaattaga accagctgga
     7681 gtaggtgcac gtagtttgca agaatgtctc atgcttcaaa cagaacgaga tgattatgcg
     7741 ccggaattag cttatatcgt gttggaagaa tgttttgagg aactggtgga gcgaaaatgg
     7801 aaagaaatcg cacaacgttt ttctgttgat ctacatgctg ttcagcagat attcgactat
     7861 ttacaaacgt tgagtccaag ccctggccgg attttcgacc gtagcagcga attgtacatt
     7921 attcctgatg tccgggtatt agtggacgac gataaaaatg tacaagttat ctcaaaccga
     7981 aaaaatcagc caaatatacg ctttcaagag agttacttca aacaaatgtc acaacaagca
     8041 gataaagaaa ctgaaaatta tttaaaagaa cggaaacagg aattcgaatg gttgaagaaa
     8101 acgatcctac aacgtgggga tacaattttg cgggttgctc aagtcattgt ttcccggcaa
     8161 aaagctttct ttatcgacaa ggaacgtcca atcaagcctc ttacgttaaa ggaagttgca
     8221 actgagatag atgtgcatga atccacggtc agtcgtgcag tcaacgggaa atatttggaa
     8281 acagacttcg gtgtatttga attaaaaaaa tttttcacta cacgaattcc tacgaatagt
     8341 acagaacaga cagaagacct ttcagcagat acagcgaaga aaaagctgca ggaattagtc
     8401 gatcaagaag acaaaaacaa gccgttgtct gaccagaaat tggttgaatt gctgaaaaaa
     8461 gatgagatag cgatctcaag acgaacagta gcaaaatatc gtgatttatt aggaatcccg
     8521 agttcatcaa aaagaaaacg ttatgataac taatcagtca aaagactaga aaaagtattt
     8581 cactttttct agtcttttta gttgaaaaac gagagcaaaa ctgttagact tacagtcgtg
     8641 aggttgagat ttccctttat tgatgtgaaa ttcgaatagt taatttcttt ttttatcgta
     8701 tatgggtcgt tttaagtcta cattggacgt aaagtgtcca actaaggaga tcgctatgct
     8761 ggatgaaata aaaatgattg aatctgttgc accagatatt atcgaagtgt tgcaagaacg
     8821 ctataaaatt ttacgaaata tctattggat gcagccgatt ggacgtcgaa gtttatcgga
     8881 aagtatgggg atcacagaac gagtattgcg aacagaaaca gatcttttga aaaatctgaa
     8941 tttgatcgac acctcaaaaa gcggtatgac gctgacaact aagggcgaag aagtttatca
     9001 aagccttgaa aacttcatgg atcagctttt aggtacgcat caaaccgaac aacagttggc
     9061 agaatatttt ggtaaccaac gctgtatcgt tgtttcagga aacagcgaag agcagaccaa
     9121 agtggctgat gcattcggcg aagcgctgag cgaagcccta gaccgactac ttccagaagg
     9181 cgagaatatc atcgctgtga tgggaggaac tactatggct gtggtagccg aacaattgtc
     9241 taatctggaa aataagaaac ggcataacct gtttgtccct gcaagaggcg ggattggtga
     9301 agcgataact gtacaggcaa attctgtcag tgcaagaatg gctgctaaag caaacggtaa
     9361 ccaccgtgca ctttatgttc cggaacaatt aagtctggca acttacaatt ctttactcaa
     9421 tgagccatct atccaagaag tcttaaactt gattagtgaa gcgaattgtg ttattcatag
     9481 tattgggcgt gcattacaca tggcagcacg tcgtaagatg actgaaaaag aattagtcat
     9541 gctgaaacaa gcaaatgctg ttgcggaatc ttttggctac tttttcaatg aaaaaggtga
     9601 agtcgtttat aaggtcccta gaatcggcat agaattgcaa gatttggaaa aagtcccgat
     9661 catcatggca atcgcgggcg gaaaatcaaa agccaaagca attcgagctt atatgaaaaa
     9721 tgcaccaaaa caaacgtggc tcatcactga tgaagctgct gcaaatgaga ttttaaaagg
     9781 ggtaaccctt taaaataaat atttttttga ttttcataag gaggaaatct taaatgacag
     9841 ttaaagtagg tattaatggt tttggacgta ttggacgtct agcattccgt cgtatccaag
     9901 atgtagatgg aatcgaagta gtagcaatca acgacttgac agatgctaaa atgttggcac
     9961 acttgttaaa atacgacaca actcaaggac gtttcaacgg aacagttgaa gttcatgaag
    10021 gttcattcaa cgtaaacgga aaagaagtta aagtattagc taaccgcaac ccagaagaat
    10081 taccatgggg cgaactaggc gtagatatcg ttctagaatg tactggtttc ttcacttcta
    10141 aatcagcagc tgaaaaacat ttaacagctg gtgctaaacg cgttgttatc tctgctcctg
    10201 gcggaaacga tgttccaaca atcgtttata acacaaacca cgaaacatta actggtaaag
    10261 aaacagttat ctcaggtgct tcatgtacta ctaactgttt agctcctatg gctaaaacat
    10321 tgaacgacaa atttggtgtt gttgaaggat taatgacaac tatccacgct tacacaggtg
    10381 accaaatgac tctagacgga cctcatccta aaggtgactt ccgccgtgca cgcgctgctg
    10441 ctgcaaacat cgttcctaac tcaacaggtg ctgctaaagc tatcggttta gtaattcctg
    10501 aattgaacgg taaattagac ggagctgctc aacgtgttcc tgtaccaaca ggttcattaa
    10561 cagaattagt aacagttctt gaaaaagaag taactgttga cgaaatcaat gcagcaatga
    10621 aagaagcttc aaacgaatct tacggataca acacagacga aatcgtttct tcagatatcg
    10681 ttggtatgac ttacggttca ttatttgatg ctacacaaac taaagtaatg acagttggcg
    10741 acaaacaatt agttaaaact gttgcttggt acgacaacga aatgtcatac actgcacaat
    10801 tagtacgtac tttagaatac ttcgctaact tataagatag acaaacgtac aagtgtaaat
    10861 tatagacatg tacatttaaa agcggggaag caatcgcgct tcttccgctt tttttgataa
    10921 taagtttatt caggaggaaa taaaatggct aagaaaacag tgaaagatat cgatttaaaa
    10981 gacaaaaaag ttcttgtccg tgttgacttc aacgttcctt tgaaagacgg cgtgatcact
    11041 gacgacactc gtatcaaagc agcattgcca acaatcaact acgttctaga acaaggcgga
    11101 aaagcaatcc ttttctctca ccttggacgt gtaaaaacag aagaagataa agaaggaaaa
    11161 tctttagctc cagttgcaaa acgtctaggc gaattattag gtaaagaagt aacattcgta
    11221 cctgaaactc gcggagaaca attagaagaa gcaatccgca acatgaatga cggcgacgtt
    11281 gtcgtattcg aaaatacacg atttgaagat attgacggta aaaaagaaag caaaaatgat
    11341 acagaactag gtaaatactg ggcttcatta ggcgatgtat ttgttaacga tgctttcggt
    11401 acagctcacc gtgcacatgc ttctaacgta ggtatcgctt caactggtat cccaacagtt
    11461 gctggattct tgatggaaaa agaaatcaag ttcatcggtg aagcagtaga agaaccaaaa
    11521 cgaccaatga ttgcaatcct tggtggcgca aaagtttctg ataaaatcgg cgtaatcgaa
    11581 aacttgattc ctaaagcaga taaaatcttg atcggtggcg gaatgactta cacattctac
    11641 gaagcaaaag gaatcaagat cggtaactct ctagtagaag ctgataaagt ggaactagca
    11701 aaagaattaa tcgaaaaagc aggcgacaaa cttgtcttgc caatcgacaa tgtctgtgca
    11761 ccagaatttt caaatgatgt tgaaacacaa gtaatcgaag gagacattcc tgatggtttg
    11821 atggctttag acatcggccc agcatcagta aaattatttg ctgatacatt acaaggtgca
    11881 aaaacagttg tttggaacgg acctatgggt gtatttgaaa tgagtaactt tgctaaaggt
    11941 acaatcggtg tttgtgaagc aatcgctaac ttagaaggcg caacaacaat catcggtggc
    12001 ggagactcag ccgcagcagc agaacaatta ggatttgctg acaaattcac acacatctca
    12061 acaggtggcg gcgcaagctt ggaattgctt gaaggtaaag aattaccagg actagcagca
    12121 atcaacgata aataaacgac caagtttgct caattctgac gagtaatcga tcaaaaggtg
    12181 aagggagttt tcatgactcc ctagccattt ttcaataaat taaggagtgt gttcctcaat
    12241 gcgtaaacct attatcgcag gtaactggaa aatgaacaaa actgcatcag aagcaaaaga
    12301 atttgctgaa gcagtaaaaa acaaaatccc ttcaaatgac aaagttgatt cagtcatcgg
    12361 atctcctgca ttattcttac aagaattaat gacatcagct gaaggtacag aactaaaaat
    12421 ctcagcacaa aactgctact gggaaaactc aggtgcgttc actggtgaaa cttcaccagc
    12481 agctcttgct gatctaggtg ttcattatgt gatcatcgga cattcagaac gtcgtgaata
    12541 tttccatgaa acagacgaag acatcaataa aaaagcgaaa gctatctttg caaatggtat
    12601 gacaccaatc ctatgctgtg gcgaatcttt agaaacctat gaagcaggca aaactgctga
    12661 atggatcgaa ggacaaatta cagctggttt agctgatctt tcagctgaac aagtgtcaaa
    12721 catggttatt gcttatgaac caatctgggc aatcggtact ggtaaatcag ctgacgcaca
    12781 aatcgctgac gaaatttgtg gagttgtacg tcaaacagtt gaaaaacttt atggaaaaga
    12841 agtatcagaa gcagttcgta tccaatacgg cggttctgta aaaccagaaa atattgctga
    12901 atacatggca aaagaaaatg ttgacggtgc tttagtcggt ggcgcaagct tgcaagcaga
    12961 ttcattcttg tcattgttag acgctgtaaa ataattgttg aaaatttgcg aaaaatgcaa
    13021 gaaatgtttg cactaatgag caaaattaac taaaatcgta tatgagggaa atcccttagg
    13081 aaaaaacaaa ctcaaaggag agacaaaaca tgtcaattat tactgatgtt tacgctcgcg
    13141 agatcttaga ctcacgtggt aacccaacaa tcgaagtaga agtatacaca gaaagcggag
    13201 cttttggccg cggaatggtt ccttcaggtg cttcaactgg tgaatacgaa gcagttgaat
    13261 tacgtgacgg agacaaagct cgttatggcg gtaaaggtgt aactaaagct gtcgacaacg
    13321 taaacaacat cattgctgaa gcaatcattg gctacgatgt tcgtgaccaa atggctatcg
    13381 acaaagctat gatcgcttta gacggaactc ctaacaaagg taaattaggt gcgaacgcta
    13441 ttttaggtgt ttctatcgct gttgcacgtg cagctgctga ctacctagaa gtacctttat
    13501 accactacct aggcggattc aacacaaaag tattgccaac accaatgatg aacatcatca
    13561 acggcggatc acatgctgat aactcaatcg acttccaaga attcatgatc atgcctgttg
    13621 gcgctccaac attcaaagaa gctctacgta tgggtgctga agtattccac gcattagctt
    13681 caatcttgaa agctcgcgga ttagcaactt ctgttggtga cgaaggtgga tttgctccaa
    13741 accttggttc aaacgaagaa ggttttgaag taatcatcga agcaatcgaa aaagctggct
    13801 atgtacctgg taaagacgtt gttcttgcta tggatgctgc ttcttcagaa ttctacgaca
    13861 aagaaaaagg tgtttacgta ctagctgatt caggcgaagg cgaaaaaaca actgacgaaa
    13921 tgatcaaatt ctacgaagaa ttagtttcta aatatccaat catctcaatc gaagacggat
    13981 tagacgaaaa cgactgggac ggattcaaga aattaactga cgtattaggc gacaaagttc
    14041 aattagttgg tgacgacttg ttcgtaacaa acactcaaaa attatctgaa ggtatcgaaa
    14101 aaggaattgc taactcaatc cttatcaaag tgaaccaaat cggtacatta acagaaactt
    14161 tcgaagctat cgaaatggct aaagaagctg gctacacagc agttgtatct caccgttctg
    14221 gtgaaacaga agattcaaca atctctgata tcgctgttgc aacaaacgct ggccaaatca
    14281 aaactggttc tctatcacgt actgaccgta ttgctaaata caaccaatta ctacgtatcg
    14341 aagaccaact tggtgaagtt gcagaataca aaggtttgaa atctttctac aacttaaaaa
    14401 acaaataatt cttttctaat gaagatacat gatcgactat tgaaagatag tcgagtatga
    14461 tcacttagtt aagtaatcga actctctact tgctgaaata tcagcaggta gagagttttt
    14521 tttgcaacta gcttctgtat atgatgttta caaagaatgg tacaagaaaa gatcatcctt
    14581 gaaaaaggaa agctaacaat aactagcatc atataagagc ttttaaggat agaacagtag
    14641 aataggaatc tatgagcaag tcctctcgta taaataaaat aggagggaag tagcagcata
    14701 tttttcataa agtggctctc tttcttcatt tttactttgg ttgctcctat ttgaaaattc
    14761 gagcacactc aaatacaata aaaagaaatg gcaagggaga ggattagatg aaaaaagaag
    14821 aaaaaatcgc gattttacaa gagatcatcc gaattaaatc agtgaatgga aacgaaggtg
    14881 aagtagcagc ctatttgaat aagcttttgg caaagcatga cataacagga gagattgttt
    14941 cttacagaga tggtagagat aatttgattg ctcgttacca aaagggccaa tcaggaaaag
    15001 tgttaggcct atctgggcat atggatgtcg tggcggctgg tgatgaatcg tcttggacct
    15061 atgcaccctt tgcagcagaa atccatggga accgtttgta cggacgtggt gctacagata
    15121 tgaagagtgg cttggcagca atggtgattg ccatgatcga actgaaagag tcaggaaaac
    15181 catttaacgg aacagtcaaa ttattagcca ctgtcggaga agaagtgggt gaactaggtg
    15241 gggaacagct cacgaaagca ggctatgtag atgacttaga tgccttgatc atcggagagc
    15301 caactaatta cagtttgatg tatacgcaca tgggatcaat caactataca gtcacctcgc
    15361 acggaaaaga agcgcatagc tctatgccag accaaggtta taatgcaatc aatcacctaa
    15421 atgaattcat tacgaaagca aatgctgaaa tgaatcacct cgctgaaaca atcgagaatc
    15481 ctgtcctagg aaaaacaatc cataatgtta cattgatcag tggcgggaat caagtcaaca
    15541 gcatcccaag ccatgcgcag ctccaaggga acattcgatc gattccagaa tacccaaatg
    15601 ataaaatcat tgcattgctg caatcaatcg tcaatgaatt gaatcaagag acagactatc
    15661 atttggaatt gatgatcgat tacaacaaaa tacctgtcaa agctgatcca gattctcctt
    15721 tgattcattg cattcagcaa caattcagcc agccattacc tttagttgga gcggcagcaa
    15781 caacggatgc agccgaattt acgaaagcta accattcctt cgactttgtc gtctttgggc
    15841 ctggtgtcgt tactttgcct catcaagtag atgagtatgt tgaaatcgat aattatttgg
    15901 atatgattga aaaatatcaa ggaattattt tatcctactt agcgtaagat ttttttaata
    15961 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
    16021 atttttcttt ttgaatatat caagcgttgt ggcaacatag tgatgactaa tgaaacaacg
    16081 acgatagaca acaaacgatc aaggtagtct gtacctgcct gtaccaagat cacacttgtt
    16141 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
    16201 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
    16261 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
    16321 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
    16381 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
    16441 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
    16501 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
    16561 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
    16621 tttcataatc gtttcttctt gtgcatttaa aagaacggtt aaaaactttc ggaaaaaaag
    16681 acgagtatct acttttgtca taattttact gttagcaggt ttctgccaaa attcataacg
    16741 atcaaccaat gtttgtccac gacttattcc ttgagtttcc acagcagtga agcttttgaa
    16801 accttcacag atggccccct cgataaaata agcaacagct aacggatcat tgatgacaca
    16861 tcctaaaatt ctttcttgtt tccagtggaa gtcaaaataa aaacgagtga tcgcttttag
    16921 atactctccc tcttcaggat tcatttgaca gcagtattcg aggatagtcg gtgtcaagac
    16981 gatttctctt gtcacatcta acccaaccat ttctatcgtt tgtttgagat tctcgaatac
    17041 ataagaagct gcttcaggat cacaccagta attgtattca gcaactggcg aacagttacc
    17101 atggcttttg taagttccac ccatcgagac aaaacgatcc atatgtttcc caatatttgg
    17161 gttgactttc aaagcaaaag cgatattggt caaaggaccc aaagcaatga ctgatgtatt
    17221 tgtcttttct ttgaaataat cagccaaaaa atcaacggca tggagaggtt ctgcctgttt
    17281 tctcaagatc atcgggaaat ttgtttcccc taaaccatcc ataccatgag tgtcttgtgc
    17341 actgatgaaa ggttgcttca aaggtttttc cattccttga taaacaggaa tgtctaaacg
    17401 atcacagcgt tccaggcatt tcagcacatt ctgcgtacct atatctgcag ggacattccc
    17461 acaaactacg gtaatagcaa ctacttcgat atcaggggat ttgacagcta atgataaagc
    17521 taaagtgtcg tcgatccctg gatcgcaatc aataattact tttcgcatta tttccaatct
    17581 cctttcttta gagatactta gttttttatt ctgggaagtt tccgaaccag tccaagacga
    17641 gcgtcttgat tataatgcac gaaattagta tactggcttt tattgcgaat gtaaatgctc
    17701 tcttacaagt aaatctattg aggagaactt taagtaaatg atacaatgaa aaagaaatgg
    17761 aatttacgtg tgccgaaaat aaagaatcct atggatcggt acattggata atagttagat
    17821 tatagtttgg cagaaagcgg cgggtgtaca atggttattt tttatttcat tcttattctg
    17881 ataattatag ggatcagtta ccattttctt tcttattttt atgcaaagaa attagtaaga
    17941 gcgggagtac agagaggata tgactggtat gaagacactg gtcatcgttt gatggaccct
    18001 gctgcagtca cgtcagtaga aaaaaaacga caaaaattag aagaagagtg tgaaagcttt
    18061 tgggagcagg gaacacccaa aacaatcaaa agttatgacg ggttgaaact agctggtcag
    18121 atgttcatcc atccaaataa gcagaataaa tgggtcattt gcgtacatga ttaccgaagc
    18181 acagggaaaa gagacatgtc tcatataggt aaaagatatg cagagaaagg attcaacgtt
    18241 cttgtacctg atttacgtgc gcacggagaa agtgaaggag aaatcatcgg aatgggatgg
    18301 ctagatcggc tagatttaat tgcctggatc caactcatct tagatgaaca gccgaatgcg
    18361 tccattattt tacacggcgg atcaatggga gcttctacga tcatgatggc aagtggtgaa
    18421 aaacttccat cagcggtcaa aggatttatt ttagacagcg gatatgtttc tgtttatgca
    18481 gaattccgat atatgttaag taaaatcact gtctttccta aaaaaatggt catgagatat
    18541 gcgaatcatt atgcccagaa atacgccggt tactccttaa agcaagcatc agctacgaga
    18601 cagcttggca gcaaccatct gcctttattg gtcattcatg gtgaaagaga tcattttgtc
    18661 cctacagaag cagcgtacac gattcaaaat gctacggcag gggataaagc actgctgctt
    18721 gttccagaag cagaacactt ggaagcatcg atgaaagatc cgaaaaccta ttggacagtg
    18781 attttttcat ttattgaaca aagaataacg atgaattaat tttctcttct tttttgtaag
    18841 taattcgagg ataaataatt gtttatagat caggagactg ctaaaataag gtaggaataa
    18901 aaaaatatca gttatttgag gagtgcggca tatggaaatc aaagaagaaa aaaatcgtct
    18961 tgctttattc aatgatgaac aacaagagat cggagaaatg acatggtcag atgcgggccc
    19021 agacatcatg atcattgacc atacatttgt agatccagca tatcgtggtc aaaagttagc
    19081 tgaaaagcta gtgtatactg gagtagaatt agcaagacga gacgggaaaa agatcattcc
    19141 tttatgccct tatgcaaaaa aagagtttga aaagaaacca gaatatcaag acgtcttacg
    19201 tcagtcataa gaataacagc tgaaagtttt gaactttcag ctgttattcc atttatatag
    19261 agttgtcacg gacttttatc tatgataaag ttaaagagac gatatctttt gggaaggagg
    19321 ctgcgatatg tataatttaa ttttgggaat cgtgatcgtt ttatcgattg ttatggtcat
    19381 tgcgattatg atgcagccaa gcaagcaaaa tagtgcagcg agtgcattta ccggcggtgc
    19441 ggatcaacta ttcggcaaac aaaaagctcg cggctttgaa gctgtcatgc agcgatcaac
    19501 tgcagttatg ggagctatct ggatgatctt attatttgtc ctagcatatt tatcttcgaa
    19561 gtagttgaat aagcctctcg caaaacggcg ggcggctttt tttattgcaa agagaacgct
    19621 taagaagaag ttatgaaaga aactatggta aaatgaggcg aaagaggtga gacgagtgag
    19681 acaattacca aaaccccttt atgcaaaaca tggaaagcga gcggttttgc tgcttcacgc
    19741 ttactcagga agtccaaacg atgttcgaat gctcgcacga tttttggaaa aagcagatta
    19801 tacagtttat gcgccgcttt ttaaaggaca cggaacaatg gagccatatg atattttgca
    19861 agaaaaagca gaaagctggt gggcagacac taaaaaagcc atccattttt tacagtctga
    19921 gcagttttct gatattgccg tattaggatt atcaatgggc ggcatctttg ctgtacgagc
    19981 actagaagaa gaatcagtga ttggcggcgg ttttttctgt tcgcctttgt ctcctgtaaa
    20041 aacaaacgtg cctgaaaact ttgaaaaata cgtacgtcaa gtgctgaaga cagcaggtaa
    20101 atcagagaaa gaaatcaatg aaaaagcagt tgcttaccgc tcgttagctg aacaacaatt
    20161 aatggatata caagatcagg cagcaatcgt agaaagtagg ttgtcagata tccaacagcc
    20221 gatattttta gctcaagctg ggaaagatga aatgatcgac cctaatggcg tatttgaaac
    20281 tgcacgcaag ttgagccgtc agcgagtgac acttcgatgg tatcctgaaa gtggtcatgt
    20341 gattacagtt ggtacagcaa gacgcgaatt agaaaaagac gtcttggaat ttttagaaaa
    20401 attaccttgg aatgaggaat aaatatgaca aaagaaacga taaaagagaa aattctcttt
    20461 ttcatggaaa gcagtcgcaa acgcagtttt tctatggaag aaatcgctga aggtttaggg
    20521 ttccaaaaaa gcgatgattt taaattacta gtacaaacga ttgctcaaat ggaaagagaa
    20581 cagagtatag ttttcaataa aaaaggaaaa gtgaaacttc ctttaaaacc aatcttgatc
    20641 gaaggaacat ttcgagcaaa tgaacgagga tttggatttg taacgattga tcccgaagaa
    20701 gatgacatct atatcccaaa agaagcgact ggttttgcaa tggatggaga caccgtagca
    20761 atcgatatca tgaagcgagc aaatacagat atggatcgcg gagcagaggg gaaagtagtc
    20821 gagattcgag accgggcgac tactcagtta gcaggggaat tcgttgctta ttcagaagaa
    20881 gaaatacaag aaacggatct ttacggcgtt gttatcccga aagatagaaa gctcaaccaa
    20941 tttaaagtat atgcagcagc agagggagtt cgtccagttg acggaagtat tgtgctgatc
    21001 gaactgaccc attaccctga aaaagggtat gcgactagtt tagaagggat aatcaagcaa
    21061 gtgattggtc ataagaatga ccctggcatg gatatcctct caattgtcgt ggctaatggt
    21121 atccctacga aatttcctga tgatgtattg tctgaagcag atgaagttcc agatcaaatc
    21181 agcgaaaagg atataactgg acgtagagat ttaagaaatc agctcattgt tacgattgat
    21241 ggagaagatg caaaagatct agatgatgca gttacggtta aaaaattaga taacggtaat
    21301 tattttcttg gagtacacat tgcagatgtt tcttactatg tgacacaagg cagtcaattg
    21361 gacagagaag catatgaaag agggacaagt gtatatttga ctgatcgagt gattccgatg
    21421 atcccgcaac gtttatcgaa tggtatctgt tcactgaatc cccatgttcc aaggctgacc
    21481 atgagttgcg aaatggagat tgatccaaat ggacaagtag tctctcatga tattttccca
    21541 agtgtgatcc aaacaacaga acggatgacc tacacagctg tcaatcaaat cttagaagat
    21601 caagacgaag aaacgatgca acgctatgct cagctcgtac ctatgttcca agaaatgggt
    21661 gaattgcatc gaatccttga aacgatgcga atcaaacgtg gcgcgatttc ttttgaggat
    21721 cgcgaagcaa aaatccttgt tgatgctgaa ggacacccaa atgatatcca gctaagaagc
    21781 agaggaattg gtgaacgctt gatcgaatct ttcatgcttg cggccaatga aacggtagct
    21841 gaacattata ataaattaaa tctgccgttt atttaccgaa tccatgaaca gccaaaagaa
    21901 gaaaaaatgc agcgcttctt tgattttgct tcagctttgg gcattctggt ccgaggaacg
    21961 aaaggaacga tcacaccaaa agatttacag catgtgatcg aaggagtaga agacaaacca
    22021 gaagctgcag ttatcaatac tatgttgcta agaagcatgc aacaagcacg ctattcagaa
    22081 gacaattttg ggcattatgg cttagcggca gattactaca cacactttac atctcctatt
    22141 cgacgctatc ccgatctaat cgtccatcgt ctgatccgtt cttacagtga agataaaagt
    22201 gaaagcaatc aaagttcgtg ggctacggaa cttcctgaaa ttgcagatca tagttctaag
    22261 atggaacgtc gtgcagtaga aacagaacgt gaagtagatt ccatgaagaa agcagaattc
    22321 atgatggaca aagtggggga agaattcgat gggatcatca gctctgttgt gaaattcggc
    22381 ctgtttatcg aactgccgaa tacagtagag ggattgatcc atatcaatga attgaaacag
    22441 gactacttcc attttgttga aaatcattta gctcttgtag gtgaaagaac tggtctgact
    22501 tttaaaatcg gacagaaagt gcgagtaaaa gtagtcaaag ctgatccaga tgaacgagca
    22561 gtagattttg aacttgtttc cgcagaagag gttacagcgt tggaacggcc aaaaacacga
    22621 aatggaaaat caggaaaacg cgaaggaaaa caagcaggaa aacgcccaga aagaaatggc
    22681 aagaagaaca aacgatatag cgatagtaag caaacgttcg gtaagaaaaa agggaagaag
    22741 ccattttaca aaggtgttaa aaagaagaaa aagaaaaaat aaaatgagtt ggaggaaaca
    22801 gctatgccaa aaggcgaggg aaaattaatt gcacaaaaca agaaagctcg ccatgattat
    22861 tcgatcatcg acacgatgga agcagggatg gtcttgcaag gaaccgagat caagtcgata
    22921 cgaaacagcc ggatcaatct aaaagatgga tttattcgcg tccgcaacgg agaagctttc
    22981 ttgcataatg ttcatatcag tccttatgaa caaggaaata tttttaatca tgatccgttg
    23041 cgcacgagaa agttattatt acacaaaaaa caaatcatcc ggcttgaaaa tgaattgaaa
    23101 aatactggaa tcactgttgt tcctttaaaa gtctatattc gtaacggcta tgccaaggta
    23161 ttgattggtc tggcgaaagg gaaaaaatct tatgataaac gggaagattt gaaacgaaaa
    23221 gatatcgatc gacaaattga tcgaacatta aaaaatttct ctagataatt tcattatcct
    23281 ttttcgcata ttcggtgctt ttgcttttac ttaatgataa aatttgttag catttaatct
    23341 gataaaacat gattatcata ggttttttct tagatcagga gcaccgtgta taaagaaaaa
    23401 ttcgcttcgt ttttgactga aataaaaaag tgtctaattt tcatgaaaat gttttgaatt
    23461 ttctgtttca tggtggtatg ataccgtgtg gttagaaacc gattggaaaa aaccagatac
    23521 gcacatgaat tgattttatg tgaatttcgg ggaaaagagg tgaaatggat tggatgaacg
    23581 ctcgctaacg ttccacattg gaccaatttg gtttgatggt acagtttgta taatggtgtt
    23641 actgacctgt ttgattgttt tcttcttagc ttactttttt acaagaaatc ttaagttgaa
    23701 accaacaggc aaacaaaatg cgatagaatg ggtcattgat tttagtcgag gaatcgtaac
    23761 agataatctg cctagaaaag aattgagcaa tttccatttg ttggcattta cattgttttt
    23821 atttgttttt gttgcaaaca acatcggttt agtaacaaaa atcgttttac ctaacgaaac
    23881 aacactttgg aaaagcccga cagcagaccc gtttgttact ttaactttgg catttataat
    23941 gatcttactt actcacttat ttggggtgaa aaagttaggc ttcaaaggat atttcgtaaa
    24001 ttcgttcttg aaaccatata gttttatgtt tccgatgaaa ctgattgaag aatttacaaa
    24061 cttattaaca cttgctttac gtctttatgg gaatatttat gcgggggaag ttttgctaac
    24121 cttgattgca aaaatggtaa taagcttagg ctggttctca ttgccgttag caattccatt
    24181 agagatggtc tggatcgcat tctccttgtt tatcggaagc atccaagcat ttgtcttcgt
    24241 tactttatct atggtttata tgagccataa gattgaagtg gaagaataat attcgtttta
    24301 aaaacaaact actatttatc taatttagga ggaaaacaat tatgaattat atcgcagcag
    24361 caatcgcaat catgggagca gcaatcggag ccggttatgg taacggtcaa gttatctcta
    24421 aaacaatcga atcaatggct cgccaacctg aaatgtctgg tcaattccgt acaacaatgt
    24481 ttatcggggt agctctagtc gaagctgttc ctatcttagg ggtagttgtc gccttgattc
    24541 tagtttttgg tgtttaattg aatcagtatt aaggcagaaa caatttggtt tgttactgct
    24601 tttttacatg aaaactagaa gtacttcaaa gaaaggaagg ctcgcctatg ctgaatcaat
    24661 tggcaattgc agaagttggc aatgcgatgt taggaaatgt catcgttgtc agcggctcat
    24721 ttttgatact actggctctc ttgaagcatt ttgcttgggg accaatcagc gatattttga
    24781 aaaaacgtga agacaagatc gccaatgatt tggattctgc agaacaatct cgcatcaact
    24841 cagcgaaaat ggaacaagaa cgcgaacaac aattgttagc ctctcgttct gatgcagctg
    24901 atatcatcaa aaatgcgaaa gaaagtggag aattaagccg ccaaaatatt ttgaaggatg
    24961 ctcaagaaga agcagctcgt ctaaaaagta aagcccaagc tgatatcact gtagaacgtg
    25021 attcagcgct gaactctgta aaagacgatg ttgcagaact atctcttcaa atcgcggaaa
    25081 aaatcttgaa caaagaatta tctccagaaa tgcacgaatc attaatcaat caatatatcg
    25141 aaggtctagg ttcttcaaat gaaactagat aaatatacag taggaagacg ttacgggaaa
    25201 gctttatttg aactggcgat cgattcaaat aaagcagaag aaatttatca agatctcgta
    25261 agcttacgtc aaatctatga acaagtacct ggattaggta atatgttaag tgatgttcgt
    25321 ctagaacctc atgaaaaacg agaaatcatg gacaagcttg ttagtggcta tgatggaatc
    25381 gttaaaaact ttttagaagt tgtttatgcg tataaccgaa tggatgatct tttgttcatg
    25441 atcgaagaat atgaacgtcg atatgatgaa catcaaggtt tgctgttggg aagtgtcaca
    25501 acagcagttc cactttcaga cgaacaacga atacaattag aaaagaatgt tgcaaaaaca
    25561 atgaactatc aatcagtcga attaaaacaa atcgttgatt cttctattat tggtggcgcg
    25621 attgttgaag ctaaccatcg agtaatcgac ggaagcattc gcacgcaatt agaaaatatg
    25681 cgtaatcaat taaatagata gtacaaagag gtaggtgaat cgaatggcca tcaaagcaga
    25741 agaaattagt gccttgatta aagaacaaat tgaaaattat caaaacgtgc tttcagtcga
    25801 agaaatcggt accgtaactt atgtaggtga cgggatcgct cgtgcacatg gtttagaaaa
    25861 cgcgatgagt ggtgagttgc ttgaattttc aaatggctca tacggaatgg cgcaaaactt
    25921 agaatcaaat gatgtaggta tcattatcct tggcgatttc gagtccattc gtgaaggaga
    25981 taaagtaaaa cgaacaggaa agatcatgga agttccagtt ggagaggcct tgattggacg
    26041 ggtagttaat ccgctaggtc agccaattga cggactaggt gaaatcgtta ctgacaaagc
    26101 tcgtccagta gaagcaatgg caccaggcgt tatgcaacgt aaatctgtta acgaaccaat
    26161 gcaaacaggt ctaaaagcaa tcgacgctct tgtaccaatc ggacgcggcc aacgtgaatt
    26221 agttatcggt gaccgtaaaa cagggaaaac ttccattgcg atcgatacga tcatcaacca
    26281 aaaaggtcaa gatatgatct gtatctatgt agcaatcgga caaaaagatt ctacagttcg
    26341 tacacaagtt gaaacattga aaaaatatgg cgcaatggat tacacaatcg ttgtgaatgc
    26401 cggtgcgtct caaccagcgc cgttgcttta tatcgcacca tatgctggta ctgcaatggg
    26461 tgaagagttc atgtacaacg ggaaacatgt cttgatcatt tttgatgatc tttcaaaaca
    26521 agctgttgct tatcgtgaac tttccttgct gcttcgccgt ccaccaggtc gtgaagctta
    26581 tccaggggat gttttctact tacactcacg cttattggaa cgtgcagcaa aattaagtga
    26641 tgatttaggt ggcggttcaa tgactgcctt accatttgtt gaaacacaag ctggagatat
    26701 ctctgcttat atcccaacaa acgttatttc gatcactgat ggacaaatct tcttagaaag
    26761 cgacttgttc tacgcaggta ctcgtccagc ggttgatgcc ggactatctg tatctcgtgt
    26821 agggggttct gcgcaaatca aagcaatgaa aaaagttgct gggactttac gtctagactt
    26881 agcaagttac cgtgaattag aagcatttac acaattcggt tctgacttgg atgcagcaac
    26941 acaagcaaaa ctaaatcgtg gacgccgtac agtagaaatc ttgaaacaaa aattgcatgc
    27001 accgctagct gttgaaaaac aagtagtgat tttgtatgct ttgacacatg gattcttaga
    27061 tagtatccct gtagacagta ttttagactt cgaacatgaa ttattcgaat atctggatac
    27121 caatcattca gatatctttg aaacaatccg tacaacaaaa gatcttccag aagaagaagc
    27181 gttgaacagt gcgattcagg aatataaaga catgttctta gctacaaaag gaaataattc
    27241 ctctacagaa gataaattga aatccattca aaatgcatag tgaggtgaga tgaatgggtg
    27301 cttcattaaa tgaaatcaag acacgtatcg cttcaacgaa aaaaacgagc caaatcacgc
    27361 gggcaatgca gatggtgtct gcttctaagt tgacaaaatc tgaagcttct tcacagaagt
    27421 ttcaggttta tgcaacaaaa gtaagagaaa tcgttacgca tttgactgct actcagttaa
    27481 atgatattgc ctcagaaaat cctagagggg atatcaatta caacagcatg ttgctcagcc
    27541 gtcctgttaa gaaaacgggt tatatcgtca tcactgcaga tggcggttta gtcggtggat
    27601 ataacagctc gatattgaaa caaacgatgt ctattttaga agaagaccac caatcacccg
    27661 atgattacgt gatgatcgca attggcggaa ctggtgcaga cttctttaaa gcacgcggta
    27721 tcaatctagc atatgagctg cgcaatctat ctgatcagcc aagctttgat gaagtccgca
    27781 aaatcgtaaa tatggcaaca actatgtacc aaaacgaagt attcgatgaa ttatacgtat
    27841 gttataacca tcatatcaat tcgctaacta gtcagtttcg tgtagaaaaa atgctgccaa
    27901 tctcagactt agatccagaa gaagcaacta ctttcgaaca agaatatatt tttgaacctt
    27961 caaaagaaga aatcttgtct cagttattgc ctcagtatgc tgaaagtttg atctacggtg
    28021 cgatcgttga cgccaaaaca gcagaacatg ctgctggtat gacagcaatg aaaacagcaa
    28081 ctgataatgc tgctaatatc attgatgagc tgacggtttc ttacaaccgt gcaagacaag
    28141 gggctattac ccaagaaatt accgaaattg ttgccggagc ttcggcacta gaatagtatt
    28201 gagattggag gaaaaaagat gagttcaggc aagattgttc aagtaatcgg tcccgttgtc
    28261 gacgtggaat tttctttaga ccaatcctta ccagatatca acaacgcatt agttgtttat
    28321 aaaaatgacg aaaataaatc aaaagttgtt cttgaagcag cgttagaatt aggggacgga
    28381 gtgatccgga ccattgcgat ggaatcaaca gatggtttac aaagaggaat ggaagtcatt
    28441 gatacaggca aagcaatctc tgttcctgta ggtaaagaaa cattaggtcg cgtattcaac
    28501 gtactaggag atacgatcga tttagaaaca cctttcccag aagatgcgga aagaagcgaa
    28561 attcataaaa aagcaccagc ctttgacgaa ttaagtacaa gtacagaaat tttggaaaca
    28621 gggatcaaag ttatcgattt gcttgcccca tatttaaaag gtgggaaagt cggactattc
    28681 ggtggtgccg gtgttggtaa aaccgtactg atccaagaac tgatccataa tatcgcccaa
    28741 gaacatggtg gtatttctgt atttaccggt gtaggtgaac gtactcgtga aggtaatgac
    28801 ttgtattatg aaatgaaaga ttcaggagtt atcgaaaaaa cagccatggt gttcggacaa
    28861 atgaacgaac caccaggtgc acgtatgcgt gttgctttga ctggtttgac gattgcggaa
    28921 tatttccgtg atgtagaagg tcaagatgta ctgttgttta tcgacaacat tttccgtttc
    28981 actcaagctg gatctgaagt atcagccttg ttaggacgga tgccttctgc ggttggttat
    29041 caaccaacat tggcaacaga aatgggtcaa ttgcaagaac gtatcacatc tacgaaaaaa
    29101 ggttcgatca catcgatcca agcaatctat gtgccagccg atgactacac tgacccggcg
    29161 ccggcaacag cattcgccca tttggatgcg acaaccaact tggaacgtaa attaacagaa
    29221 caagggattt atccagcggt agatccattg gcttcttctt caagtgcttt agcacctgaa
    29281 atcgtgggag aagaacatta taaagtagca acagaagtcc aacacgtatt gcaacgatat
    29341 cgtgaattac aagatatcat tgctatctta ggtatggatg aattatccga tcaagaaaaa
    29401 gtgttagttt ctcgtgcccg cagagtccaa ttcttcttgt cacaaaactt taacgtggct
    29461 gaacaattta ctggtcttcc aggttcatat gtccctgtag aagaaacagt gaaaggattc
    29521 cgcgaaatct tggaaggcaa atacgatgat cttcctgaag aagctttccg aagtgttggc
    29581 cgaatcgaag acgtcgtaga aaaagcgaaa acactaggct actagaaagg ggtgccccat
    29641 ggatcaatat ttaacagtaa atgtggtaac tcctgccggt ttggtctatg atcaccatgc
    29701 agctatcgtc gttgcacgta cgacagctgg tgagcttggg attttgccta aacatgcacc
    29761 aatcatcgtt ccattgacga ttgatgaagt tcgtgtaaaa cgaacagatt cagataccca
    29821 cgtcgattgg atcgctgtta atggcggaat catggaagtt cgtgataatg ttgtttcaat
    29881 cgttgctgat agtgcggaac gtgaacgtga tatcgatgta tctcgtgcgg aacgtgcaaa
    29941 acaacgcgca gaacgccaaa tcgaagaagc aaaagcaaaa caagatacga acgaattgaa
    30001 acgggcaact gttgcgttgc accgagcaat caatcgtatc aaagtatcta agcactcata
    30061 aaatgagatg cggatgttta tccgtgttta agaacagtaa aaagaggtta tgacagaagt
    30121 ggtcagcttc aagacataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
    30181 ctcagcttat ttttcgaaga agctacttct ggaacaccgt atatcggagg ttgtgaaatc
    30241 agcgttttgt cctgagtatt agagaaacaa atggaaaata gttcctcata ttttgccgtt
    30301 ttttgagcta ataccgaggg tcagatgatt gaacaccgtt tatttggaat aagggactgc
    30361 gacaagactt ttgtctcagt cccttttttt gcatattttt agcatttatt tcctaaaaaa
    30421 tgtaagggga ttcctatatt tttaagtata cttgtttttc cttttataca ttttatggta
    30481 ttatcatttt attagttatt ataaaacaat cgttagaaag gacgtcatta tgcaagtata
    30541 cggaattgat gcggtgcttc gaatcgtgag tcatttggcg tttatctact tagccttctg
    30601 gtctttacgt tcgttacgca ttgaaacttt tttcagacca atgcataata cgcaaattag
    30661 aatggcaatc gtactttttt caattttttt agggtacact gcaagtaatt ttttcttaga
    30721 agttatcgct ttatgccgaa atcttttcgt tagcctgtaa attttaaaaa aaggtactat
    30781 tttaaaagca aggatgttat aatagaaaag attttgagta agtgtatgct cattggaatt
    30841 ttttggaggg aacaacatgg aagagatcat cgtaagaggt ggcaatcaat taaatggaac
    30901 agtaagaatt gaaggagcta aaaatgctgt tttgcctatt ttagctgcta gtttgttagc
    30961 agaagaaggt attacgacat tagacaacgt tccgattctt tctgatgtct tcactatgaa
    31021 tcaagtgatt cgtcacttaa acgtagatgt agattttgat gaacaaaaga atcaagtaac
    31081 aatcgatgct tcaagacaat tagaaatcga agcgccttat gagtatgtta gtcaaatgcg
    31141 tgcttcaatc gttgtgatgg gtccgctact tgccagaaat ggacatgcaa aagtagctat
    31201 gcctggtgga tgtgcgattg gtaaacgacc aatcgatttg catttaaaag gatttcaagc
    31261 tttaggagca aaaattattc aaaaaaacgg ctatatcgaa gcaattgcgg atgaattgat
    31321 cggtaacacg atttatttag atttccctag tgtaggtgct actcaaaata tcatgatggc
    31381 tgccgtaaaa gcaaaaggaa caaccatcat tgaaaacgta gctcgcgaac cagaaatcgt
    31441 tgatttagca aacattttga ataaaatggg cgctcaagtc tatggagctg gtactgaaac
    31501 aatgcgtatt gaaggcgtgg atcatttaca tgcagtcaac cactcaatcg tacaagatcg
    31561 tatcgaagca ggtaccttta tggttgctgc tgcaatgaca caaggcaatg tgctgattgc
    31621 agatgctatc tctgaacata accgcccatt gatttctaaa ttgatcgaaa tgggtgcaga
    31681 aatcattgaa gaagaaggcg gagtccgtgt catcggacca aaacatatct tacctacaga
    31741 tgtaaaaacg atgcctcatc ctggtttccc tacagatatg caagctcaaa tgacagctat
    31801 ccaattagtt gcagaaggta caagtgtagt gactgaaaca gtgtttgaaa atcgtttcca
    31861 acatttagaa gagatgcgcc gtatgaacgc tcatgtcaaa atcgacggaa atgtagcaat
    31921 catggatgga aaccatgaat tgcaaggtgc agaagtatat gcaacagact tgcgggcagc
    31981 agcagcatta gttttagctg gcttgaaagc aaatggaatc acacgtgtgc gtaacttgaa
    32041 ttatctagac cgtggatatt ataatttcca tatcaaacta cagcaattag gtgcagacgt
    32101 tgaacgtgta gacatggatc aaacttctgc tgaaaaaaca gcacaaacga ttgcttaagg
    32161 aagtggctga atgagtaacc aacgatatat cttattaact ttgataaaga ttttagtggt
    32221 gatcttattg cttatccttc tttttgtggc cggaacgatg atcggatatg gtgtcatcgg
    32281 tggcggcaat ccatttaaag tattccagcc aagtttgtgg attcatattc gagacttttt
    32341 tcattgataa ataagaatca cagtggttct agtcgatatg aagtgagcca tgaagcagaa
    32401 catgacaaaa gaaaacagaa gggaacgttg acgttagtcg cgatcccttt ttttagtaaa
    32461 agaaaagaag gaatctaatt gatgaagaag atattgattt atttagttag aggatatcag
    32521 cgctttattt cgcctgcatt tccacctagc tgccgttatt acccaacctg ttccaattat
    32581 atgataaaag caattcaaac ccatggtgca ctaaaaggga cggtaatggg gactgcacgg
    32641 atcttacgct gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt gacattccaa
    32701 ttaacgcgaa ataagaaaga taaaaaagaa ccagattata aaaaggttgt gaaaaatacg
    32761 agtagcactg agcgataaag aaacaatttg gaaaatagct tcttatattt tgcaaaattg
    32821 tgagttattg ccgaagtgtt ggattttgaa caccgtcaaa aaaaggttgt gaaaaatacg
    32881 ggtagcactg agcgataaag aaaaagagga aaattattgt caaagtaaca actccgcaac
    32941 tacttatatg ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc
    33001 ttgttattag atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt
    33061 ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa
    33121 aaagaatacg aaatcgtcaa tgtagcagta gccccaacgt atcaaggaaa ggggattggc
    33181 ggaaaattac ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag
    33241 atcatcatac gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc
    33301 tatgtggaga ttggtcgagt caaagactat tttatccatc attactctga gcccattttt
    33361 gaaaatggag atcaattaag ggatcaagta attttagcaa ttcacttacc taaaaatagc
    33421 tttaactaat aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag atacattaca
    33481 atagctttag caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt
    33541 ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt
    33601 gcaacgcctg aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata
    33661 ctcgtttctt ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag
    33721 aaacaggtga ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga
    33781 gatttggaaa ctctgtttca actcccagta acgattgaaa atgacgccaa ctgtgccgga
    33841 atggctgaat tttatgaagg tgctgcccgt gattatcaag atgttgcttt tgttgtggca
    33901 ggaactggga ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt
    33961 tatggtggag aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt
    34021 acagcggtac agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaattcttat
    34081 accggaaaag atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt
    34141 gagagttttt atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca
    34201 gaagtgatcg tattaggcgg cggtgtttct gcaaaagaag ggctcattga tgagatcaaa
    34261 agccgaatga agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta
    34321 ttatgtgaat acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta
    34381 cgtcaagggg agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga
    34441 aatgaatagg agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct
    34501 gagaggtgaa aatgatgtca aaaaaaaata aagatgttga agtgcgtata gaagaagtga
    34561 aaaaagatat caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga
    34621 ttggtgagat cttagcctat gggccaaaag aatatgaaat tttctttggt gaagaagatt
    34681 ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc
    34741 acgaataaaa aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag
    34801 gtattataac taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg
    34861 cgacggactg taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc
    34921 attggggtat agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa
    34981 tccagctacc ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tct
//
LOCUS       DACEOM010000028        34670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEOM010000028 DACEOM010000000
VERSION     DACEOM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.029"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(322..2214)
                     /locus_tag="IW781_001267"
     CDS             complement(322..2214)
                     /locus_tag="IW781_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182271.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2211..3938)
                     /locus_tag="IW781_001268"
     CDS             complement(2211..3938)
                     /locus_tag="IW781_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182272.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3954..4604)
                     /locus_tag="IW781_001269"
     CDS             complement(3954..4604)
                     /locus_tag="IW781_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1182273.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4808..5104
                     /locus_tag="IW781_001270"
     CDS             4808..5104
                     /locus_tag="IW781_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1182274.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5296..5916
                     /locus_tag="IW781_001271"
     CDS             5296..5916
                     /locus_tag="IW781_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1182275.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6008..7174)
                     /locus_tag="IW781_001272"
     CDS             complement(6008..7174)
                     /locus_tag="IW781_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1182276.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7186..7539)
                     /locus_tag="IW781_001273"
     CDS             complement(7186..7539)
                     /locus_tag="IW781_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1182277.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7667..8689
                     /locus_tag="IW781_001274"
     CDS             7667..8689
                     /locus_tag="IW781_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1182278.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9128..9562)
                     /locus_tag="IW781_001275"
     CDS             complement(9128..9562)
                     /locus_tag="IW781_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182279.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9672..11531)
                     /locus_tag="IW781_001276"
     CDS             complement(9672..11531)
                     /locus_tag="IW781_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1182280.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11649..12809)
                     /locus_tag="IW781_001277"
     CDS             complement(11649..12809)
                     /locus_tag="IW781_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1182281.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13116..14540)
                     /gene="araA"
                     /locus_tag="IW781_001278"
     CDS             complement(13116..14540)
                     /gene="araA"
                     /locus_tag="IW781_001278"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1182282.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14556..15254)
                     /locus_tag="IW781_001279"
     CDS             complement(14556..15254)
                     /locus_tag="IW781_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1182283.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15211..16872)
                     /locus_tag="IW781_001280"
     CDS             complement(15211..16872)
                     /locus_tag="IW781_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1182284.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17090..18490
                     /locus_tag="IW781_001281"
     CDS             17090..18490
                     /locus_tag="IW781_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1182285.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18742..19821)
                     /locus_tag="IW781_001282"
     CDS             complement(18742..19821)
                     /locus_tag="IW781_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1182286.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19907..20866)
                     /locus_tag="IW781_001283"
     CDS             complement(19907..20866)
                     /locus_tag="IW781_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1182287.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20878..22401)
                     /locus_tag="IW781_001284"
     CDS             complement(20878..22401)
                     /locus_tag="IW781_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1182288.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22411..22974)
                     /locus_tag="IW781_001285"
     CDS             complement(22411..22974)
                     /locus_tag="IW781_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1182289.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22980..23843)
                     /locus_tag="IW781_001286"
     CDS             complement(22980..23843)
                     /locus_tag="IW781_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1182290.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23857..24753)
                     /locus_tag="IW781_001287"
     CDS             complement(23857..24753)
                     /locus_tag="IW781_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1182291.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24928..26199)
                     /locus_tag="IW781_001288"
     CDS             complement(24928..26199)
                     /locus_tag="IW781_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1182292.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26189..27706)
                     /locus_tag="IW781_001289"
     CDS             complement(26189..27706)
                     /locus_tag="IW781_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1182293.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27904..28794
                     /locus_tag="IW781_001290"
     CDS             27904..28794
                     /locus_tag="IW781_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1182294.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28867..29691)
                     /gene="nadE"
                     /locus_tag="IW781_001291"
     CDS             complement(28867..29691)
                     /gene="nadE"
                     /locus_tag="IW781_001291"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1182295.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29703..31175)
                     /locus_tag="IW781_001292"
     CDS             complement(29703..31175)
                     /locus_tag="IW781_001292"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1182296.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31812..32174)
                     /locus_tag="IW781_001293"
     CDS             complement(31812..32174)
                     /locus_tag="IW781_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1182297.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32397..33149
                     /locus_tag="IW781_001294"
     CDS             32397..33149
                     /locus_tag="IW781_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1182298.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33289..34212)
                     /locus_tag="IW781_001295"
     CDS             complement(33289..34212)
                     /locus_tag="IW781_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1182299.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34227..34499)
                     /locus_tag="IW781_001296"
     CDS             complement(34227..34499)
                     /locus_tag="IW781_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182300.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10232 a         7216 c         5980 g        11242 t
ORIGIN      
        1 accgtttacc aagaggttgt gagcctgccg tttaactccg agtcacaagg agcaatttca
       61 aaaaacagct tctcaggttt gttgactatt gtgacttgtg accgaggagt tggcaggaga
      121 acaccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagatacaaa
      181 acggaaaaat agcttctcat attttgccgt tttttgagct aataccgaag gacagctgat
      241 tgaacaccgt ttattcggaa aaagggactg tgacacgact tttgtcacag cccctaaaaa
      301 aaataataaa tttgtactat tctatgaagc ttcttcttca gaaaactgac tgttataaag
      361 atctgcatag aatccgtctt tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga
      421 accatgattc atcacaataa tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac
      481 aacgaaactt gtccggtttt ccaataggcg gttcatcgct ttttgaatca gtatttctgt
      541 tcgtgtatct acactagatg ttgcttcatc aaggatcagg acatctggat ttgctaagaa
      601 tgcccgcgca attgtgatta gctgacgctg cccttgcgaa atattgcttg catcctcgtt
      661 taatacagtg ttatatcctt ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt
      721 agcagcctca atgatttgtt cgtctgttgc atcgtcgttc ccatatttga tattgtcata
      781 gatacttcct gtaaacagcc aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc
      841 atcacgtgaa agatcacggg tatctacacc atcgtaacga atactacctc cagaaacatc
      901 gtagaaccgt tcaagaaggt tgatcagtgt cgtcttccct gcaccagtcg gacctacaat
      961 agcgaccatc tcaccaggtt tgacatccaa gttaaaatca gtcattagca agttatctgg
     1021 tgtgtagccg aattgaacat tttcaaaacg aactttgtat ggtgtatctt tttcttctgg
     1081 aaggccagat ttttcattgg acatctcttc ttcatccaac acttcaaata cccgttcggc
     1141 tgaagcaact gttgattgga tagtgttcat caaacttgca agttgagtga ttggttgaga
     1201 gaactgtgtt gtatattgca agaacgcttg aacatcccct aatgtcatgt tcccatttgc
     1261 aacttttact ccgcctagta cagcgacgaa cacatagcct agatttttaa cgaaattcat
     1321 caacggcatg atgattgccg aaataaattg agctttccaa ccagcggcat acagattctg
     1381 attttctttt tcaaatactt cctggtcttt ttcagtatga ttgaatgttt taacaatggt
     1441 atgaccacca tatgtttctt ctacttggtt attcaataaa cctaaacttt tttgctgatc
     1501 tgcgaaaaac acttgtgatt tcggcgctac gatcatcacg atgatcaagc ttaaagggat
     1561 catcaataac gcgactaacg tcaattgcca gctgattgaa agcatcatca cgatgacccc
     1621 aatcaatgtg actgcactag ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat
     1681 gttatccata tcattgattg ctcgagacat gatatcccca tttgtatgtg tatcataata
     1741 ggaaatcggt acacgattca ttttggcttc caaatcatga cgtaattcat aaactgttcg
     1801 ttgggaaaca cgtgtcataa taaattgctg taaaaagttg aatacagcag aaatcaaata
     1861 catcacgatt actgtcgcaa tgatctgcgc gattttatca aagtctatcg gaaattttgt
     1921 tacttgttgg ccttgttgca tctgttgagc gccagccatt acacctttga atatttctgt
     1981 ggttgctttc cccaagacct ttggtgtttg gatttggaag atcacggctg ctattgctag
     2041 tacaaataca gcgatgatcg caaccatacg tttagacata tagccaaaca atcggcgtac
     2101 cgttccccag aaatttttag gcttttcacc ttttacaccg agatttctgc caggtccacc
     2161 atgtcctgga ccgcctgctg gtccatgaga gggacgtgtg tttgttttac tcatgctaag
     2221 tcctcctctc ttagttgtga atgaacgatt tcttgataag tttcgtttgt tgctaatagt
     2281 tcttcatgtg ttcctttgcc aaccagtttt ccttcatcta gcacaaggat catatctgca
     2341 tccattactg tacttactcg ttgagcaact aaaacggtga tactttcttt catattcgtt
     2401 ttcaatgctt ggcgaagatt ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca
     2461 aacacataga tatctgctgt tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt
     2521 ccaccagaga agtttcctcc tccttgttcc acatggctat ctaagccatc ttccatctct
     2581 gaaacaaaat ctttagcttg tgcgatttct aatgctttcc aaatcatttc atctgttgca
     2641 tccggcttac catactgcat attgtctcgg atcgttccag agaacagaac tgctttttga
     2701 ggaacaaagc ccattaattc tcgcaaatta tattgagtca tgtcactaat gtccgttcca
     2761 tttatttgga tcgaaccaga ttcgatgtca tataatcgag ggatcaaatt gactaaggtc
     2821 gttttacctg aaccagtccc ccctatgatt gcgacgatct cgcccgactt ggcttggaaa
     2881 tcaatatctt ccaatgcaag tttttctgct ccatgatatc gataattgac atgattgaag
     2941 gccaatgttg cgttttttcc agcaaaagaa actgttttag gattttcagg gtcatggata
     3001 ccgatttttt catccaacac ttcattaata cggtcagctg aagcttgtgc acgaggcacc
     3061 atgataaaaa tagcggaaag catcataaag ctgatcaaga tctgcatcgc ataagtcata
     3121 aatgcaatca gattaccgac ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa
     3181 gtgattgcta cgtttgtacc gctcatgatc aatgtcatca ttggcatcat caatgcgacg
     3241 atcgtattaa ctttgattgc cgtatttgta taatctttat tcgctaaatc aaaacgattt
     3301 tcttcaaagc gtgtcttatc gaatgctcga atcactctga cccctgtcaa tccttctcga
     3361 aataccaggt ttaaacggtc agttttcttt tgcatacttt tgaataatgg aacagcaaag
     3421 tacatgattc cgccgatcaa aataatcatt actgggatga caactaagaa gatttttgtc
     3481 aactgatgat ctttttgata agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg
     3541 atcatcatac gtaaaaacat ttgtgtaacc atttggattt gagtcacatc attggtcgtt
     3601 cttgtgatca aagaggccgt tccatattta tcaaacgcat cttttgtcag actctctgat
     3661 tttttataga catccgtacg caattgctta cccagtttct gtgattcacg agtagcaaaa
     3721 aatgtatttg cgatagcagc taagatactg atcaaagaaa agccgatcat cacaaaacct
     3781 gtatgccaaa tataatcgac atctccttgc gctacccctt tatcaataat attggaggta
     3841 agtgtcggca aatataaatc tgccagtact tggatcacca tgaaaactgc agctgcaatt
     3901 gctgaagtca aggagatccg ttttacgatt tttagcatct agttcccttc tttctattta
     3961 tatactccgt ttttcagcca agaaagtttc aaattgaatt cactgattgc ttgctctact
     4021 gagtaatctt cagatgttga aaataagcga tacgaatgcg caattgttgt aaatacagta
     4081 ttcatcacta attggatcag taattccaat tcatgatcat tttttacttt cagcttcgtc
     4141 cgatcgatca attcaagcaa ctcttttgca tcttgtttgt gttctttatg tggatcgtga
     4201 ggcgtttttt ggcgactcgt atgaggagcg acttttcttg aggcacggta atccatgttc
     4261 ataaacaagt tacgataaaa agaagcattc tcgccttcca aaatctcata aatcatttta
     4321 gaaaaatagg attcgaatcc tttgaataga tcaccatcgg attctttcat tagttgtaac
     4381 atatctttag agctgtcacg tctcattgtc tcaaagtaat aaaaataaag atcctcttta
     4441 tcttcaaaat actgatagaa actgcctcta gggatatccg ctaatttgac gatattagca
     4501 atcgaagctt cttttaaggg tactctagaa aactcgattc tcgcagcctc gattagtctt
     4561 ttttgttttt ctttaggcaa atggaaaaat gtctgtgtcg gcatatggat tctcctttct
     4621 ttcaagatga cacattgtca tatgacgcta tgtcattata acacctccta cctgttttgc
     4681 aagacatttg catagttttt ttcattatca tccgatacat taaaaaagtg ataagaggaa
     4741 tccttccatt tgaattaatg agagttgccc attaaaatag ccacatacta aacaaaggat
     4801 gagataaatg gcccaatcaa ttagtgtcca agacttcaat aaattgcctc tcgatgctga
     4861 cagcatcgtt ttagatacaa gaaatacctt agattacaca aaaaaccact taagccactc
     4921 gctttctatc cccgctgcta tcttgccaag acaattaaaa aatctagatc caaagaaact
     4981 ttattatatt ctaagctata caggcaggcg atcagaagca ttagccgatc aacttactgc
     5041 taatggtttt cgtgctgttt atatcattgg tgggatggaa gctcttcagt cgagtgttgc
     5101 ttgaatattg cttgttagat tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt
     5161 ttataccctg tcttttttta gttatttatt cttcaacctt atcaatatga cttgatagcc
     5221 ttttcttttt cgagtaaact gattttgtta gtcatagtta taacaatcat agcaataaaa
     5281 ggagaaggta agcatatggc atctgtttta gtagtcaaag gtcatcccct aactgcagaa
     5341 gaatcgcgca cagttaaggc attgacgtct tttttaacaa gttataaaga aaatcatcca
     5401 gatgatgaag tgactgtttt ggaactttat cgcgatgata ttccggaaat cgatgaagaa
     5461 ttattatcag gttgggaagc attacgtgct ggtgcagaat tcacttcctt atcagaaagt
     5521 caacaacaaa aaattgctcg tttcaatgaa ttgactgatc agttccttgc tgctgataaa
     5581 atcgttatcg caaacgcctt atggaatcta aatatcccaa caaaattaaa agcttggttc
     5641 gataccgtca atgtagctgg aaaaacgttc cgctatacag aaaacgggcc agaaggatta
     5701 gtgacaggta aaaaagcact gcacatccaa tctaatggcg gtgtatacaa cggacaagac
     5761 tttgcctctc aatatgtaaa aggtatttta aactttgtag gcatcgatca agtagatcag
     5821 ctattcatcg aaggaatcga ttatgaccct gaccgtgcag atgagttgat gcaaaatgca
     5881 ttagataaag ctgctgcttt aggtaaatca ttttaaaaat tgagataaat gcatacaaaa
     5941 gagacagagg aaaaaatctt caaagcagca tttgtgcctt ggatattttc ccatctgtct
     6001 ctttttgtta ttctttctgc tttttcaacg gattttcttc ataaagagaa gagattgttg
     6061 tgtaccattc aaaaacatga gtaatcacta ctttagctgc tgcataaaca ggaatcccca
     6121 agatcacacc gatgattccg aacaatttcc cagaagtcag cagaactaat aaaatcgtta
     6181 ctggatggat tgctaactga ttccctagta ctaatggaga gatcaagcgt ccttctattg
     6241 tctgctctac tgcaaaaacg atcagtactt tgatgatcat gaatggacct gctacaatgg
     6301 ctaaaaagat agccggaatc atcgctaaaa atgatcctaa gtaagggatc aggtttaaaa
     6361 aacccgcaat aatccccaac gtgacagcat actctaaacc aatgaccgca aaaccaatca
     6421 taaacatgat tgccacagca aaagccacag ttagttgtcc tcttatataa gaagaaactt
     6481 gactattcat ttcactcagc acattcaacg tcggtttttg catgcgtgtc ggtaaaaatt
     6541 tgacaaaata aggtgctaat tgtttaccgt ctttcaatag ataaaataag ataaacggca
     6601 tcgtcagcag agccactaaa atagtcgcta ccgcgccgac aaaacttcca agactctgaa
     6661 ctgtactctt agatatatct tggatcatat cgcctgccga attcatgaac ttatcacctg
     6721 ctgtttccaa ctgttcgcga aattggttga acagcgggtc ccgcaaaatc tcggttaatt
     6781 tattatcaat cgtatctaca tattgaggga agttactgat gaagctcact gtttgttcct
     6841 gaatcttagg gatgatcact acgctccccc atgcgattag tgcaacaacc aaaacaaaca
     6901 gcccaatgat actgtaaagc cttggtactt ttcgcttttc aaagtaatca actaccggat
     6961 tcatcaagta atacaagatt cctgctaaaa tgataggcag acctacgatt gctaaaaatt
     7021 gccagattgg tgcaaacaaa tatgaaactt ttgtaaataa aaagacgatc agcaatatta
     7081 acaatacaac aagtaaggct gtgactactt gattatttaa aaaccagcgc caaaaccacg
     7141 ataccggtct gttcttctta ttttttcctt ccataattcc tcctgttagt tataaaaaat
     7201 ttggcttccc aaaccaatag caggcagtct atgcggatga agatagattc catttgcaat
     7261 ttgtccttct tctggacaag catcaaaaat caatgatacg tgagctactg tctttaaatt
     7321 cccattggcc atcggaccga cataatcgat cgtatatacc tgatcatcaa aagcaattgt
     7381 tccgcctgct ttcaaagaaa aaggttcgtc ttttgatact tcttgaatca ccgaatattt
     7441 tcgtaaggca gctgttgctg ttttatcgaa caaaatcagc atgggttctt ccttgtttat
     7501 tgcttctgtc ccaatttctg taatctttgc ctgaatcatt gcactctctc ctttaattgc
     7561 tttcaaaaac agtataacac attctcattc tttagccgtg tggaataatt atgtgataga
     7621 ataaaaacga aaactatttc attacaatta ggaggaatca gctcatatga tcgaaaagat
     7681 aattttagga acctatacgc gtcgcgaaag taaaggaatc tatacgatcg accttgatac
     7741 agaaaaagaa gaattagcca acctttcatt agctgttgaa gaaaatagcc ctacctatgt
     7801 agcaaaaagt aaagctggca atctttacac agttactagt gtggatggat taggtggtgc
     7861 cggtgcgtac gataaggatt ttcatttttt gaatgcggta acagagtctg gcgctccttt
     7921 gtgttatgta gcggttgatg aagcacgtca attactatac ggggcgaact atcataaagg
     7981 tgaagtaaat gtctaccata tattaggaga tggcgaactg aaagcagcag attcaatatt
     8041 ccatcaagaa gcgacaggtc ctcataaaaa ccaagatcat gcgcatgtcc actatacgga
     8101 tctgactcct gaccaacgat tagtcgtatg tgatcttggt acagatcgtg tgtatactta
     8161 tgatgtgtcc gaaaacggca aattgaatct tgtaactacc ttcactgcag agccaggaac
     8221 aggccctcgc catcttgtct ttcatccaaa tggacaattc gcttatttat ttggtgaatt
     8281 agacagcaca gtccgcgtgc tttcttataa taaagaaaca ggtgcttttg aagaattaca
     8341 gaaaatatcg accttgccag aagaattcga cggtgaaaat ggaggggcag ctatccgcat
     8401 ttcaaatgat ggcaagttcc tttatgcttc taaccgagga cataactcga ttgctgtatt
     8461 cgcagttttt gaagatggtt cccacattca aaacaagcaa atcatctcaa cagaagggga
     8521 ttttccccgc gatttcgcac tagatccaag caataatttt gtcgtgtgtg ccaatcagaa
     8581 caccgataat ctgacattat atcgccgtca tcctgaaaca ggattactgg aattactgaa
     8641 aaaagatgtc tatgtaccag aatgtgtctg tgtttatttt gatcgttagt aagaaaagga
     8701 gaatgaaatg aatcagcaag acgtattaga cagactacgg gaagaactaa atattccttt
     8761 ctttgatggg aaaatcgaag ataaagagta tagcgaagag gaatatcaga aactcaaagc
     8821 cgatttacag aactactttg aacaatatgt acagaatgta gaaaactaat cagctttcca
     8881 tactatctga aaagaacatc aggacaagac ttttccgtct atcctgatgt tcttttctac
     8941 aaataaaaaa ccgccgagat ggttagactc gacggaaaag gagtatttac tcgattttga
     9001 gtaagtacaa ggttgttgtt ggttataggt atgtcattat cataaaggat tttgatcact
     9061 ctatcataaa ggtaatatta catttatatt agataactct agtttttatt gttatcaaag
     9121 atttctttta ttttttcaac aacaaattag tcattttttg gatggcagct gtcaattggt
     9181 ctgtcgcttg gtcttgtttt gcttcagatg tattgaatag atcttcatct acccctgatt
     9241 caagatcgat actttggatc atttcttcac agcctgctac atatgaaaga taagcttttt
     9301 caaaattttt atgaatgccc attgcttttg ctggtggacg caaagtactg actttcataa
     9361 gcattgcccg atattttttt gttccttctt caaataacag gatgatctct tcaagagtcg
     9421 atttgtccag ttcacttgtt tttccttcat caattgcgtt tctgatcact tcatatttcg
     9481 gatgcattcc ttctccgatt gcttctgttt ccttgacgat ttgatcgatg gactgcacat
     9541 aatagccaat attcggtaac atgttcgttt tcctactttc tccattgttg ttattattta
     9601 ttctacttgt ttttatcctc aaatgacaag tacttgaaaa aatttaatgg tttctataca
     9661 gctcgccatt cttattcgtg ctcaaagaaa ccatctgcta aactatattg tttccctgcg
     9721 acaatgtcat attggatcaa ttgatattcg tcgtaaatct cttgttgcga gcgattgaat
     9781 tgcgcttctt tataccactg tccgttttga tagtacagtt ctcgttcaaa cgccggaagt
     9841 tcctgttcta atgataaaag cagctgataa aagccatttg ttgtctgatt cgtctgttcc
     9901 attagattcg ctgcaaaata aaacggactg gtgattgcat cgtgagtcgt ttgcttttct
     9961 aattctcctt tactgtcgaa cataagaaat tgtgtttcat gcaatgtttg tttgtcattt
    10021 tttgcttgga tcgtatctga atagatccct ggaagatgat ccccccaaaa aacaactaaa
    10081 gtccttctag aaagattctt caattcttcc gtgaactgct tcagtgccgt gctgctatag
    10141 gaaatatctt gcagataatt ctctaagtca agtgtcccac ttccatctga agtttttgca
    10201 ggatagtcta gattatcata ctttccatcg tagggcatat gtgtctgcat cgtgactaaa
    10261 tgaatgaact gaggtgtttt ttcttccttg agaagtgtca ttacttcttt gtaagctgac
    10321 tcatcagaaa tatatggatt attctctatc gtatccgtat aggtcatcgt acgctcgctg
    10381 atgaactggt caaatcctaa tgtctgataa acatcctctc ttttatacat cgaagtattg
    10441 tatggatgga tcgctgtcgt ctggtatgat tgtgcgttta acgctgaaac gatagaagga
    10501 agctgatcca tttttggtac aagcatcgtg taaggggtgg tgagttgcgc attaaataac
    10561 gccatagaaa aaccagtcaa tgcttcaaat tcgatatttg ctgttccacc gccatagttt
    10621 tgagaaagca tattgccact atatgtttga tcagctactt cataataatc tgccaatggc
    10681 tcaccagata cttctacgcc gtttaatcga ctaggatctg agaaactttc actcatcacg
    10741 aacacgatgt tcggcgactc ttcctctaca gttttatttt tttcatcggc tagcttctgg
    10801 tatttttcag tgatttcttt gatcttttct ttcgaatatc cttcaggttc atccattggt
    10861 tctaccttca gattgtacaa aaacccaccg ataaaaccag tgttatagta gttcattttc
    10921 tggctgtaag gtatccatag tgccgtttta ttatatgctt ttcgcaacaa attatcggga
    10981 ttgttgaaat tactgatata ccctagcagt ccaattgcca caagcagtgt cgttaatcgg
    11041 atcaattgga atcgccttcc ttttttcagg cttcgaaaca tgtaccagca aaacaatcct
    11101 atcacggttc ctgcggcagc taagatcact gtaaacagca tagtgccggt catatctttc
    11161 agcaatccga tttcagtgat catcttcaaa tcgtctggat aaatcggttc ttgccgataa
    11221 ctcattttca tataatttgc aaaacctaat acaccgatgg ctacgacata cgtaagggaa
    11281 cctaaaggga cagatcctgc tagtgcaatc agaaaaataa gaataatcag caagacgaga
    11341 caggctaaga agaatttttc cgtatgccaa gaaaaagcaa atttcaacgc aaggtctacg
    11401 gatagatcat tttgacacca ctgcaaaaat aaattactaa aaacaacaag aaaaaagata
    11461 ctgatgattg ctgctgttcc ttttaaaatc tttttctgtt tggtcgaaag tctgcttata
    11521 tatcttttca aatgcttgct ccttcatttc tatcaataaa agaatgttgc ttctcttatt
    11581 ttagttgtac gaaattcgaa agtaaagaac ggaaagaaga attaatgaaa tccaaatatt
    11641 ctctttcttt atggcctttc tggtttcttt aataagcgga cctttcgtac tccagaaatt
    11701 ttttctagct gttgtttcac ttgattgagc tgttcaaaat cagtactgtc tatgtcaatc
    11761 aatatgtaga catgctcatt cttatggtta cgcgatatat ctgcgatcgc tagctcgttc
    11821 tcattcagca gagaaaatat ctctgctaat atattggttt tcttttgata aaatatagtg
    11881 aatcgaaacg gcgcttggaa aaacattcgt gcggatggat aattgaccgc ttctctaacc
    11941 gtaccgaaca aaagataatc ttttaaggct tcaactgcca aacgtccgct gtctgccaaa
    12001 gcttcctgag tcgatccccc tagatgaggc aacataagta tacggtcatt ttctaagaac
    12061 tcttcttctg gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata
    12121 acagcatatt tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc
    12181 attttttcta tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg
    12241 actacgatgt aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc
    12301 aactgggcat cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc
    12361 gctacttttg tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct
    12421 cgtcccacat aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt
    12481 gtttgtacca tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa
    12541 acaagctctt ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt
    12601 tcagaagctt tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc
    12661 aaatctggtg taagccattc atctttcact ttcgtactac gaataactat tccttcggct
    12721 ggcaaatcat ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga
    12781 atagcatcaa tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga
    12841 aatttctgca attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta
    12901 gagcagagta ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat
    12961 tttcgagagc aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca
    13021 ttgataataa gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat
    13081 ttcttaaaac taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac
    13141 tcaactaatt ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta
    13201 tgatgaccgc cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt
    13261 ttccataaca cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct
    13321 acttcattga tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct
    13381 tctcctgctt tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa
    13441 ggagaaacaa cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg
    13501 gattccaaga ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct
    13561 gtatctttcc ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg
    13621 ccttccccag caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt
    13681 tgtttcattc catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct
    13741 aagaaacggc gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc
    13801 ttttcttcat aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga
    13861 tcgacttctt cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa
    13921 tcaacagtcc aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg
    13981 tttctcatgt tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact
    14041 gcaacatcca tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg
    14101 acgattttat tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga
    14161 gcggattgat ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta
    14221 tattgcgtag ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt
    14281 tttgctggtg agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg
    14341 acctctttca tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt
    14401 ggataaggca attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt
    14461 acttctcgca gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct
    14521 ttttctccaa tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca
    14581 ttttttccgt gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa
    14641 aatgaattga gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg
    14701 ttcattaccg cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt
    14761 acccctggaa ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt
    14821 cctgtttcat attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt
    14881 accgatccat aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct
    14941 tgtgcccaga tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat
    15001 gaacgatata aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg
    15061 atatgattgt ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact
    15121 ccgctaggtt tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat
    15181 gtatatttga cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc
    15241 agttgttcca gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc
    15301 aatgtcgatc ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc
    15361 tgaaggtgtg atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc
    15421 taaagacttc ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc
    15481 accttctccg gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg
    15541 ctgtccaact tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac
    15601 attctcagat ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg
    15661 gatccgcatg aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct
    15721 tccttcattt actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc
    15781 gcaatccgta tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct
    15841 tatctccata cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc
    15901 cgaagagcag ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat
    15961 gtcgatttcc ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga
    16021 agtaccagcc gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt
    16081 tcctgcatcc ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc
    16141 gatcagataa gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt
    16201 aggcaaaatg tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat
    16261 atttttcatt tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc
    16321 atctcctacg cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt
    16381 tgtcaaaaaa ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg
    16441 agcgatactc catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc
    16501 ttcttcagtg attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc
    16561 aaatgctaga taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt
    16621 ctcgccatag atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt
    16681 ccaaatatct tctaatgaat aggtccagat accattttct agtttgtttt cccactcata
    16741 actaccagcg gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga
    16801 tccaaactca atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt
    16861 tacttccttc ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt
    16921 tagtagcgct tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac
    16981 aagaatacaa taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta
    17041 caaaatctga tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga
    17101 aaaaaaagtt tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg
    17161 atatgatatt ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc
    17221 aggtaaagct tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg
    17281 cggctcttta tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc
    17341 tgcacttatt tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta
    17401 ttttctgatt gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt
    17461 tcctgcttat atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa
    17521 tcaagtgatg attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg
    17581 gttgcctgaa acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat
    17641 tttatttttc ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg
    17701 cttggaagag gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga
    17761 attcgaacaa atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac
    17821 tttgttttta gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca
    17881 gcaatttcaa ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac
    17941 tgggcatgca gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc
    18001 tggttctttg ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat
    18061 aggcggtagc gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga
    18121 tgctcataca aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc
    18181 ttgcacatgg gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg
    18241 cgggcgagca tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt
    18301 gctttttcct gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat
    18361 catctgcttt ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc
    18421 tttagaggaa atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca
    18481 tcattcttaa agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt
    18541 agtttccagt cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg
    18601 acttgtgacc agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca
    18661 gccttgagcg atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa
    18721 gaggcggtaa aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag
    18781 tacaggttca tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca
    18841 ccgtgcggca tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt
    18901 ccctaaatgg cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg
    18961 atagccattt tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat
    19021 gatgccatct aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc
    19081 tcgtgtttcc gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc
    19141 acacgcgctg ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat
    19201 gaacaacaga cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt
    19261 cacatcatct aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac
    19321 catcggaatg ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt
    19381 acttttcgta ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca
    19441 ctctttctcc aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc
    19501 tcgtaattca ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt
    19561 ggtgatgaca ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc
    19621 atctacatat gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc
    19681 gattgcttgc cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga
    19741 gggaatggtt gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc
    19801 aatatcttga tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata
    19861 acgagtctgc aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg
    19921 gaacaggttc accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg
    19981 cgtgccgatt aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat
    20041 agatcaaaat atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa
    20101 attgcttatt tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt
    20161 ttaataaaag attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg
    20221 ttgcactacc agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt
    20281 catttactga aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct
    20341 tcccctttaa cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa
    20401 tttcaggatc ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc
    20461 cgtctaaact gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat
    20521 tcattggatc tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac
    20581 gtatagtttt atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga
    20641 cttcaggtgc ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca
    20701 ggcttgcagc atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt
    20761 agcctggaac cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat
    20821 ctgcccgttg ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca
    20881 cattttcttt attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc
    20941 atgtttttct attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt
    21001 atttttgtcc aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt
    21061 gaaatcatgt tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt
    21121 cagctgattg ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc
    21181 acaatcataa acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga
    21241 taagtgttgc atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag
    21301 tattaaaggt attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg
    21361 tcgtaaaata gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca
    21421 ccaatcgatg ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac
    21481 attccattca tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata
    21541 atcacaagta gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta
    21601 tgtcgctgta tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt
    21661 atcaatatag ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat
    21721 acgatcccat tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc
    21781 aattgaatca tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc
    21841 ataagcactc ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa
    21901 tttaacgcca aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc
    21961 ttttggaaaa ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa
    22021 attcaccgtg aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag
    22081 tccaaaagaa tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc
    22141 tttcctacct acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg
    22201 aatgacacct aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc
    22261 atcagctgtt tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc
    22321 aacaaatgag ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt
    22381 tactttcact tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat
    22441 agatacaagt ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat
    22501 gagcagtaca aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa
    22561 tactttccat gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat
    22621 cacatatatc caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat
    22681 tgaaagtacg attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt
    22741 taacagacta aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata
    22801 aatgatattt cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt
    22861 agcaggcacg atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat
    22921 gtaatcagaa accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct
    22981 cactctttca ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa
    23041 accactagta atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa
    23101 atgttctgcc ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt
    23161 gtcaatatca atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat
    23221 gataaaagag taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt
    23281 aatccatcaa tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc
    23341 atcatgaaga taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca
    23401 agcatgccaa gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc
    23461 atcattgtca gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca
    23521 aatgcatacc ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa
    23581 atcactgtat ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta
    23641 tctagggtat agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt
    23701 ttaaaagaag aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc
    23761 atactaatcg ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact
    23821 gtaatcgagc tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg
    23881 tgacatgtac acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga
    23941 cagtgctgat gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg
    24001 aacagctgtt tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa
    24061 cacttgcatc gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg
    24121 aggtagcgta atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc
    24181 atacaaagag ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa
    24241 attcttccaa acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa
    24301 ttgagggatc ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc
    24361 aagcaacata gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa
    24421 tgtcgaacga caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa
    24481 accaacgata atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga
    24541 attaactgct cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct
    24601 tgatgtaaag aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat
    24661 gacagtaaat aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc
    24721 aggacgattc caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat
    24781 aaaatataca atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt
    24841 tttgcaataa cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca
    24901 acagtctatc ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc
    24961 tgcatcgttc atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt
    25021 gatgatatcg ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata
    25081 gtccatttgt ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc
    25141 tactgaatcc aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt
    25201 tgtattccaa tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc
    25261 cggaatggca tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg
    25321 tggtaaggta gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg
    25381 cccattgatt tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga
    25441 aatcgattct ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt
    25501 aaggttctca ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc
    25561 atttgctaac atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga
    25621 tacattttta ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt
    25681 aggtggttgt tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata
    25741 catgccttgt acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat
    25801 agccgtttct ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg
    25861 ttgtaccgct ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc
    25921 aggtgctttt ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat
    25981 gatatccatt tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc
    26041 ttgcaatatt tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt
    26101 atcagaatca gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc
    26161 taccaacaaa gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg
    26221 ataaacgaca tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat
    26281 cttcagcttt tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag
    26341 ttgcttttgt atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt
    26401 cagacaagtg aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa
    26461 gttctttgtc atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg
    26521 tcgcatagtt tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag
    26581 cttgcataaa tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga
    26641 ttggtgcaat aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt
    26701 tcaataaagt gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt
    26761 ctagtaaagg aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat
    26821 accaaatatt ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct
    26881 tgactgtatc gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg
    26941 caagataatg agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat
    27001 ctatttgatc gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt
    27061 gccgattcga acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct
    27121 tagccgtttc ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag
    27181 gaccgtccat ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat
    27241 tcgaacgcct taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat
    27301 tacgagctgc atctactcca cgagtaccca gattgacagc catattgatt tcggccccca
    27361 catgctgaca ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc
    27421 aagctaaatc caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa
    27481 atcctgacac aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca
    27541 agtgaatgac gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa
    27601 tcccttcata tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa
    27661 ctttgcctac acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt
    27721 aatttataca tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact
    27781 tacttatttt ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt
    27841 taatataact tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata
    27901 tatatgcaac tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa
    27961 acaaggatca agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca
    28021 aaagaacttg gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca
    28081 aacatcatca aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa
    28141 atcgatatca gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata
    28201 cctattggtc actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat
    28261 gattttatcg ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc
    28321 cgcattctct ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca
    28381 gaggatacat tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca
    28441 aatgacaatt ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg
    28501 acgagccctg gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat
    28561 aatcttaatc aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg
    28621 aatggagaac ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg
    28681 catttgcgaa tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt
    28741 aaaggattcg gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt
    28801 tcttttctat tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct
    28861 ttattgttat ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt
    28921 cttgttccac catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag
    28981 ataatcatca atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt
    29041 cccatcttca agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc
    29101 ttgaagcaat tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc
    29161 accatcacca aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa
    29221 tacggctcct gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt
    29281 gatatttcct ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg
    29341 tgcatcaaca gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag
    29401 agcagctttt gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa
    29461 ttgatagttt tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg
    29521 gcctgctaaa gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg
    29581 atattttttc aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc
    29641 aattgttggt ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc
    29701 tcctatgctt ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt
    29761 ttatggttcc agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt
    29821 ttatactctt cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt
    29881 gcaggtaatt cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct
    29941 gtaaagtctc gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct
    30001 tcttcttctc gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca
    30061 aaatttcgag tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg
    30121 cttgataact tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg
    30181 aatactgcac caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat
    30241 acatcaattt tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc
    30301 gaagcataga tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta
    30361 gatatatagg ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct
    30421 aattctttcg ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg
    30481 actaagaaca cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt
    30541 ccataagatt ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc
    30601 ccagcacgta cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag
    30661 actgctgcat ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg
    30721 atgacagatt tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc
    30781 aacagtgctg tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt
    30841 tcatttgcaa aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa
    30901 ttttgtaaat actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata
    30961 tcggaatcat tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa
    31021 accgcgtatc cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa
    31081 tcagctcgac ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc
    31141 agcgtcaaac tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt
    31201 ttcatttcct acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc
    31261 tgaaaaagca catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact
    31321 atcctttctt attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc
    31381 cgagacacga agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc
    31441 ctactacaat cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta
    31501 caacataatc aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt
    31561 atccccaaag aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat
    31621 gacaagatat accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag
    31681 atacaaagta agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca
    31741 gtctcgcctt gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt
    31801 attagtcgtt attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta
    31861 tttggacgtt tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact
    31921 ttgacatctt ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt
    31981 gtgatttttt tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat
    32041 agcagttgtg ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa
    32101 aagaattcca ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt
    32161 tcgattagct tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt
    32221 atgttaccat ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag
    32281 gatttacctt ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt
    32341 tcttgaccat ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg
    32401 agtacacaat aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt
    32461 atgatgagat tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg
    32521 gcaaaaaaga agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt
    32581 tggatgatat ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca
    32641 agcatcagca aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc
    32701 aacagacact tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag
    32761 ggtttaaaca acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa
    32821 aatacggaaa aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa
    32881 cgtatgaaga aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa
    32941 aagaaccaga agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct
    33001 cttacagctg gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag
    33061 ccgatgaacg gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt
    33121 taaatgaaat tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg
    33181 taaaatgacc taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg
    33241 tgtctgctcc attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat
    33301 aaacgtataa ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat
    33361 ttctccgcaa gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag
    33421 caaattatcg cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc
    33481 tgctgcaacc atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa
    33541 gcctgcttgt tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa
    33601 agatggaaaa agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc
    33661 acttcgcccc aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc
    33721 ttctgtaatt gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc
    33781 ttcacaaaaa actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc
    33841 acgagacgta atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg
    33901 ctctttttct ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc
    33961 tgcttcaatg atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat
    34021 cactccaatg cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata
    34081 atcgacttct ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg
    34141 tccattgatt acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg
    34201 aatgtttgcc actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc
    34261 acagctggac cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc
    34321 tgcccgcccc aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt
    34381 actatattcc tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg
    34441 acaaacgcat aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt
    34501 gcaagccctc cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt
    34561 ttgttttgcc aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa
    34621 attgaataca ttataatgat actgtaaata tatctttatt attagtgaat
//
LOCUS       DACEOM010000029        33114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEOM010000029 DACEOM010000000
VERSION     DACEOM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.030"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..897)
                     /locus_tag="IW781_001297"
     CDS             complement(<1..897)
                     /locus_tag="IW781_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1182301.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHY"
     gene            1170..3641
                     /locus_tag="IW781_001298"
     CDS             1170..3641
                     /locus_tag="IW781_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1182302.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3744..5480)
                     /locus_tag="IW781_001299"
     CDS             complement(3744..5480)
                     /locus_tag="IW781_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1182303.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5477..6181)
                     /locus_tag="IW781_001300"
     CDS             complement(5477..6181)
                     /locus_tag="IW781_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1182304.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6312..6815
                     /locus_tag="IW781_001301"
     CDS             6312..6815
                     /locus_tag="IW781_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1182305.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6775..7113
                     /locus_tag="IW781_001302"
     CDS             6775..7113
                     /locus_tag="IW781_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1182306.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7134..8552
                     /gene="ffh"
                     /locus_tag="IW781_001303"
     CDS             7134..8552
                     /gene="ffh"
                     /locus_tag="IW781_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1182307.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8664..9242
                     /locus_tag="IW781_001304"
     CDS             8664..9242
                     /locus_tag="IW781_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1182308.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9246..9767
                     /locus_tag="IW781_001305"
     CDS             9246..9767
                     /locus_tag="IW781_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1182309.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9846..10400)
                     /locus_tag="IW781_001306"
     CDS             complement(9846..10400)
                     /locus_tag="IW781_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1182310.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10653..10928
                     /gene="rpsP"
                     /locus_tag="IW781_001307"
     CDS             10653..10928
                     /gene="rpsP"
                     /locus_tag="IW781_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1182311.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10940..11191
                     /locus_tag="IW781_001308"
     CDS             10940..11191
                     /locus_tag="IW781_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1182312.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11316..12107
                     /locus_tag="IW781_001309"
     CDS             11316..12107
                     /locus_tag="IW781_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182313.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12277..12798
                     /gene="rimM"
                     /locus_tag="IW781_001310"
     CDS             12277..12798
                     /gene="rimM"
                     /locus_tag="IW781_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1182314.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12788..13549
                     /gene="trmD"
                     /locus_tag="IW781_001311"
     CDS             12788..13549
                     /gene="trmD"
                     /locus_tag="IW781_001311"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1182315.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13685..14032
                     /gene="rplS"
                     /locus_tag="IW781_001312"
     CDS             13685..14032
                     /gene="rplS"
                     /locus_tag="IW781_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1182316.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14405..15067
                     /locus_tag="IW781_001313"
     CDS             14405..15067
                     /locus_tag="IW781_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182317.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            15146..18373
                     /gene="fss3"
                     /locus_tag="IW781_001314"
     CDS             15146..18373
                     /gene="fss3"
                     /locus_tag="IW781_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1182318.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18487..18801
                     /locus_tag="IW781_001315"
     CDS             18487..18801
                     /locus_tag="IW781_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1182319.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18814..19188
                     /locus_tag="IW781_001316"
     CDS             18814..19188
                     /locus_tag="IW781_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1182320.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            19198..19542
                     /locus_tag="IW781_001317"
     CDS             19198..19542
                     /locus_tag="IW781_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1182321.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19613..20965
                     /locus_tag="IW781_001318"
     CDS             19613..20965
                     /locus_tag="IW781_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1182322.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            21025..22167
                     /locus_tag="IW781_001319"
     CDS             21025..22167
                     /locus_tag="IW781_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ1182323.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            22192..22446
                     /locus_tag="IW781_001320"
     CDS             22192..22446
                     /locus_tag="IW781_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182324.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22702..23886
                     /locus_tag="IW781_001321"
     CDS             22702..23886
                     /locus_tag="IW781_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1182325.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23901..24020
                     /locus_tag="IW781_001322"
     CDS             23901..24020
                     /locus_tag="IW781_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1182326.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            24766..26676
                     /gene="ltrA"
                     /locus_tag="IW781_001323"
     CDS             24766..26676
                     /gene="ltrA"
                     /locus_tag="IW781_001323"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1182327.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            26780..27004
                     /locus_tag="IW781_001324"
     CDS             26780..27004
                     /locus_tag="IW781_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182328.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            27017..27517
                     /locus_tag="IW781_001325"
     CDS             27017..27517
                     /locus_tag="IW781_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1182329.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            27614..29461
                     /locus_tag="IW781_001326"
     CDS             27614..29461
                     /locus_tag="IW781_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ1182330.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            29464..30360
                     /locus_tag="IW781_001327"
     CDS             29464..30360
                     /locus_tag="IW781_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1182331.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            30410..30799
                     /locus_tag="IW781_001328"
     CDS             30410..30799
                     /locus_tag="IW781_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1182332.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            30786..>33114
                     /locus_tag="IW781_001329"
     CDS             30786..>33114
                     /locus_tag="IW781_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1182333.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT        11144 a         5657 c         7081 g         9232 t
ORIGIN      
        1 ataatgccgt ttgagtggct ccaatgtcgt ggaatctttg agtaagagtt tccaatatcg
       61 cttgagccga cggtacttct tctgattttc agagtgtgag gttcgaaaac gattcatgat
      121 ctggacacgt aattgattga agctgcgatt gatgtgttgg acaatatgaa agcggtcagt
      181 tacgatctcc gcatttggaa agactgtttt aagcaactga caatagctgg cattcatgtc
      241 catcactagg tacttgacct ttaaacgggc ctttctagga aagcgcagga agtattgagt
      301 tagtttgtaa agtctgcgat cttcaaggat cgtaaatagc tgattggttg tcccatcaac
      361 acaaataaaa ctcatggaac cagaaacaga attcattgat ttgaactcat ccatacacaa
      421 caccgtcgga aggaagttca tacgtggctg gtaatgcttc gttgcttcac gcaagatccg
      481 tgtcaccgtc ccatccgaga cgaagtactt cttagcgatt agtttacgtg attgattttc
      541 gtacagttcc atgagaatca cctgcttcag ttcctttgtt agttgatgat gttcatcaac
      601 taagtccgtt ttcgcagaaa aggtccttcc acaatcatgg cagagaaaac gggtacgttt
      661 taattctaaa atagttaact tttctttgac tttcaacatc tgagtgcgtg tcgtatagga
      721 accgttgcgg ataatacgaa ccgatcgaca gtgttcacaa taggaagaag agtcatccaa
      781 ttttccttta ataaactgcg cttgaacgcc tttatattct ttttcaatca gccattcttc
      841 atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt ctagtatgat tatccattga
      901 gagactcctt tggtaggatg acgttttggt cgactaaatt ttaccaaaag tgagtctctt
      961 tttgagttga aacgaagaaa aagtgttagt agaaaatcgc cttcgatttt ccaccaacac
     1021 aaaatattat agaacccttt ttttttgcta tatgacagga atgaaaggtg tttgcaagct
     1081 taatgtgagg aacactttcc atatctgttc tatactggat aggagaagtt tggaaatatc
     1141 cagaaaacga aatcaatcga ggtagccaca tgaagaataa atggataaat cgaagtaaaa
     1201 aaaagaaagt aacacaaaga tcatcagata aagtcatccg tctttttcca tcgtataaag
     1261 aaggattaac ggcagatcaa gtcaaggaac gaatagaaaa aggtgcagca aataattcag
     1321 tcgatcccac attcaaaaca aatcagcaaa tcgtattgga aaacatattt acgtatttca
     1381 atttgatctt tcttatctta gccattttgc tttgtttagt agaatcctat aagaacttga
     1441 cttttttacc agttatcata gccaatacag gaatcgccat ttttcaggaa attcgatcca
     1501 aaaagatctt ggacgaacta aatgttttaa atacagcaaa agtcaatgtc atacgagacg
     1561 ggatagaaca aacaatagat atagaagacc ttgtaataga tgacattgtt attttagaga
     1621 ctggatatca aattccagca gatgcagagg tcgtggaagg gaaactgcaa gttaatgagg
     1681 ctttattgac aggcgaagca gatgaaataa ccaaagaaat aggtgatcca ttaatgtccg
     1741 gcagtttcat tgtttccgga aaagcttacg cccgattaga caaagttgga gcagattcgt
     1801 atatttccca gctgacagtc aaagccaaga caatgggaga aggagaacag tcggagatga
     1861 ttgcttctct taatcaattg atcaaatgga ttgggatcat cattattcct atcggtatcc
     1921 tgcttttttc tcaaagttac ttttttaatg gaaacacgat caaagaaagt gtcgttgcaa
     1981 tggaagcagc attgatcggt atgatacctg aaggactgta cttgctgacg acgattgcat
     2041 tagcattaag tgcgactaaa ctagcaaagc aaagagttct tttacacaat atgaaaagca
     2101 ttgagacatt ggcaagggta aatgtccttt gtgtagataa aacaggaacg atcacagaaa
     2161 ataaaatgtc tgttcaaaaa atgatcgtct ctaaaaaaca agaaataatc agtcagtcag
     2221 atcaattgga agcatgcatt gctgactatg caaaagcgat ggcaggagac aatgcaacaa
     2281 tggaagctgt taaagcatat tttacaaaaa ccactgatca atcctatcac catgttattc
     2341 ctttttcttc tgtccaaaaa ttcagcagtg tagcgttggc agacaaggtg tatgttttag
     2401 gagcgcccga aatggttttg cgcgatcaat tgccggaata tgcggaagag tttgtttctt
     2461 tcgctgaaaa aggctatcgc gtgttggtct ttggtgtgta cgatgggatt cttgaagaac
     2521 cagtattgac agaagcagtc acaccattag gctatattct gattgctaat cctatcagaa
     2581 aagaagcaag agccacattt gattacttca aaaaacagca agtagccatc aaagtgattt
     2641 ctggagataa tccgctcacc gtatccaatg tagctgtcca agcagggatt tcacatgctg
     2701 atcagtacgt tgatgcatct aaattatcag aagaagaata tgaagcagct gtcgaaaaat
     2761 atacagtttt tggacgcgtc aagccagaac aaaagaaaat cttcgttcaa ttgataaagc
     2821 gtaacaatac agtagctatg acaggtgatg gcgtgaatga tattctagcc atgaaagaag
     2881 cagactgcag tatagcgatg gcttccggaa atgaagccgc catgcaggcc tcacaagtgg
     2941 tcttacttga ttctgacttt tcacgcatgc cagaagttgt tgcagaagga cggcgcgttg
     3001 tgaacaatat tgagcgatca gccagcttgt ttttagtgaa gaatattttc tcctttttgc
     3061 tctcactgtt ttcagtgatc tttgctctga cctatccttt agaaccatca caaatcacat
     3121 tgattagttt gtttactatc ggacttccat ctttcttgct tgctctagaa gaaaacaaaa
     3181 agcgaatcag agggaaattc atcatgaatg tgatggaaaa agctgtgcca ggtggactaa
     3241 cagatatgat cgtagtcggt gcgttggtga tctgcggggt gactttgaat ctaaataaaa
     3301 cagatgtttc tacagcatct accatgcttt tgattgcagt agggttcctc gttctctata
     3361 aaatctgttc accgctgaat aaattcagaa gtcagatcat tcttttctgt gctagcggta
     3421 actttttttc ggtcatcttt ttgcataagc tgttttcaat caccggtatc tcagctgtat
     3481 cacttttgct gttgagcatt ctatttttct cggcagattc gatttttagg catttgacca
     3541 cattggtaga atatttgttt gaacgtaaaa atgaaaaaga gcaaaagccg tttagtcgcg
     3601 tagctaaaaa atttctatcg atatttaaac gagaaaattg acgaaaagta tctcaaaaaa
     3661 gagtaaaaaa gaacaaccac tcgtgtatct ttcgaagcag gatgagtcat ccaactttaa
     3721 aacgcacgag cagttgctct tttttacatt ttcggaatgg taacggtgaa agtcgaacct
     3781 gttccaagat gactgtctac agtaatcgta ccattaagga gttgcacgta gtcttttaca
     3841 atcgctaacc ctaatccggt cccgcctgaa tgtcggcttc gcgccttgtc tacgcggtaa
     3901 aaacgctcaa agatccgttc ctgatcctca atgtcgatac ctataccaaa gtcttggaca
     3961 gaaaacgata gatcatctgt tgcctgatag cggataataa tctcgctgtc gctttttgaa
     4021 tattggacag cattttcaat tagatttttg ataattggat aaaggatatc tgttttagta
     4081 gtaaatggtt cgttctttcc aatcaatcga atcgtcaacc gtttcttttc gatgattggc
     4141 tgataatttt gaacgatttg ctggaaataa ggttctgtat aaattgtctg gatctcgtaa
     4201 gaattctcgc catcttttga cagctggatg atttcccgaa tcagtttatc taaccggatc
     4261 gcatcttttt gcatgatctc aagaaaagaa gtcagagttt gcgggtcatc ttttgcacca
     4321 tccagtaaag tctcggtaaa accaatcagt gaagtcacag gagtttttaa ttcgtgagag
     4381 acgttgccaa cgaaatcctt ttgtagcttt tctaaacgtc gcactttcgt taagtcatac
     4441 gccaccccta gaatctgtcc ggtaccttct gaattagtaa agtagcgcaa actcaagtct
     4501 aatgtctgcc gaccttctgt aagggtgatc tcctgatgga tcagcggatg ctcaggagtc
     4561 acttgatgaa tcagctgaat gagttttggc tcttgaatga cttctgtgta ccgctgttca
     4621 ggttgatagc ttaccacgtt taaatgtagc tgcatctttg gattaagcag tagtaaacga
     4681 gaatcagcat catcaatcag gaatacgcca atcatcagtt cgttcagtaa cgtatataat
     4741 tgctcttctg tggtagtaca cgcccgatac atcttactca tttgttcgct caacgcatta
     4801 atcgtctgat acaattcttc ccattgatcg ggtgtttgca tgattacttc cgcctgatta
     4861 ggattttgca ccattttttt tagtacgggc agcacggttt tcaatggctg gtttttttga
     4921 tagatcagat aatagatcat tcctgcaaga acaaggaaga aagacagaaa gaagaaaaag
     4981 acccaacggc gaaaactctc agttctcggt aaaaaaccgc ttgtcggttc tgcgatacgt
     5041 aaaatggctt ctaactggcc actttttttg actggtaatg cgacatataa aagatcctca
     5101 ttcaaagtag tacttttgcg caaagcgcta cctaggttcc caccattcaa tacggctttg
     5161 atttcgggac gggagtttcg ctgttcattc aatgtttcat tgtttgtgtc aaataagatc
     5221 tttccagtag catccattaa agtaatccgt tcgttcgact gatggacgaa ttcttccaaa
     5281 gcagttttgt tttgcgctgc ttggaggttt tcgacatcta attgccggat cagcagttgt
     5341 gctttttttg tcaggtagct ttcttgctgt tccacgatct gctgttgaaa ataatgggaa
     5401 atcaattgcc agccaccaaa aaacaaacta actaaaagaa taagaaagaa gagacgttca
     5461 taaatttttt tccatttcat cgtttgggtt cctgaaactt atagccaaat cctctaatag
     5521 tcaccaagta ctttgggtgt tttggatctc gctcgatttt ttctcgcaaa tgactaacgt
     5581 gtacatctac gattcggctt tgtcctgcga agtcaaaatt ccaaatgcgg tctagcaatg
     5641 tgtctcggtc gatcacacgg tcttttcgtt tcatgaaata aacaagtaat tcaaattctt
     5701 tcggtgttag ttcaatcaga tggtcgtcta cggtcacttg ataattggtc aaatcagcag
     5761 tgatttgtcc aatcgataga tattcaggct cagcttcatc ttttgattgc ttacgaggtt
     5821 caattcgtct gaatattgct ttcatacggg ctaatacttc gcgaggacta aatggtttgg
     5881 tcaaatagtc gtcagcacct atttctaggc caatgatccg atccacttgg tcatctttag
     5941 ccgtcagcat caaaatcggt gtttcatttt tttcttgacg cagccgctga gtgatctcca
     6001 tgccatccat cgttggaagc atcacatcta aaatgatgaa atcaaatgat tgttctaatg
     6061 ctaattctaa tccttcgcct ccatctgtgg cactggttac cttgtagcct tctttttcca
     6121 aattgaacgt caagagcgtt acaatagaag gttcatcatc aacaactagt atttttttca
     6181 ttcaaatctc ctttaaatag gtttcattct gtttatattt tagcatagaa tcagtacaaa
     6241 gggctatttt acaaaagaaa taaatggtat aataggaagg cactttgctg aatagaaagg
     6301 aagagcagtc aatgattgga atcagtgcat gtttaggcgg tctggcctgt cgctatgatg
     6361 gacagaaaaa agaagtacca gaattgatgg aactagttga aactggagaa gctgtcatgg
     6421 tctgtccaga agtgatcggc ggattaccaa ttcctagaga tcccgctgaa atcattggtg
     6481 gaaatggatt cgatgtgtgg aaagatcaag caaaagtatg gacgacaagc ggcgaagatg
     6541 taacggaagc gtacaaaaat ggcgcgatca aagcctatca aaaattgcag gaaaaacaca
     6601 ttgatctagt catcatgaag gaaaaaagcc cttcatgcgg aacccattcg atttatgacg
     6661 gaagtttttc cggaataaaa aaaaatggtg ccggagtggc tactgcttat tttattcagc
     6721 aaaaaatgac tgtgctctct gatgaagaat ggctgaaaac tagaggtgag cagaatggaa
     6781 atcgaaaaga ccaatcgaat gaacgcgctc tttgaatttt attcgacctt attgacagaa
     6841 aaacaaatga actacatgga attatattat gcagatgatt tttcattagg tgagatcgcg
     6901 gaagaatacg aagtaagccg acaagcagta tatgacaata tcaagcggac aagcaagatc
     6961 ttggaggatt atgaaaaaaa actccatctt ttttctaatt atattgttcg agaacaggta
     7021 ttggaaaata tgactgcata tattacagat aagtacccgg aagataaaaa actacttgaa
     7081 tatgtacagc aaattcaagc aatcgaagaa taaataaaag gaatgaagaa aatatggcat
     7141 ttgaaagctt aactgaacgt ctccagcagg cgatgggaaa aataagaaaa aaaggaaaag
     7201 tatcagaagc tgacgtaaaa gaaatgatgc gggaaatccg ccttgcctta ctagaagccg
     7261 atgttaactt gcaagtagtc aaagatttta ctaagcgcgt acgcgaacgt gcggtagggg
     7321 cagaagtgct ggaaagttta tcacctgcac aacaaatcgt aaaaatcgtt gatgaagaat
     7381 tgacgatcac acttggttct gaaacagcag agttaaataa atcaccaaaa ataccaactg
     7441 ttatcatgat ggctggtctt caaggggcag gtaaaacgac ctttactggt aaattagcca
     7501 attatttaaa gaaaaatgaa aatgctcgtc cgctcctaat cgcaggggac gtatatcgac
     7561 cagctgcgat cgaccaattg aaagtattag gtcaacagct agacgttcct gtatttgata
     7621 tgggaactga agtcagcccg gtagagatcg tccgtcaagg gatggagtta gcaaaagaaa
     7681 agaaaaacga ctatgtattg atcgatacgg caggacgtct gcatatcgat gaaacattga
     7741 tggatgagtt gaaacaaatc aaagaattga cgcaaccaaa tgagattttg cttgttgtcg
     7801 atgccatgac tggtcaagat gccgtcaacg tagctgatag cttcaaccag caattaggta
     7861 ttacaggggt cgtcatcact aaacttgatg gggatactcg aggcggggca gcactatcga
     7921 tccgctcagt gacaggcgca ccaatcaaat tcatcggttc tggtgaaaaa ttgactgact
     7981 tagaagtatt ccacccagat cgtatggcta gccgtatcct tggtatgggg gatatgctga
     8041 cattgattga aaaagcgcag caagactacg acgagaaaaa agcggaagaa cttgcacaga
     8101 aaatgcggga aaattcattc gatttcaatg actttattga acagcttgat caagtgatgg
     8161 gtatgggccc actggaagat ctaatcaaaa tgatcccagg aatgaatcaa gtccctggaa
     8221 tcgaaaacgt caaggtagat ccaaaagatg ttgaacgcaa aaaagcgatg gtttattcta
     8281 tgacgccagc agaaagagca aatcctgatc tgttgaaccc aagccgtcgt cgccgaattg
     8341 ctgccggttc tggaaacagt gttgttgaag tgaatcgaat gatcaaacaa ttcaaggaat
     8401 caagaaaaat gatgcagcaa atgtccaaag gggatatgaa catcccaggt atggaccaaa
     8461 tgtttggttc aggcgtcaaa ggaaaactag gcaaaatggc gatgaatcga atgatcaaga
     8521 aaaataagaa gaaaaagaaa aaacggaaat aagatgaaaa aaacagcttc ggtacagact
     8581 gaaaatgtct actgccggag cttttctatc agcaattttt attctttact aatacaatta
     8641 ctctaaacga agaggtggca cagatgagat gtccgtggag tgaacaaaca gaaagtatgc
     8701 ggcaatatca tgatactgtc tggggtgttc ctgaatatga tgaccaaaag ctgttccgaa
     8761 aattgatgct tgatattaat caagccggat tgagctggca gacgatttta aataaaagtg
     8821 aagcattcga tgaagcatat gatcgattcg atatcaaaaa agtcgcaaat tatggagaaa
     8881 caaaaatcag tgaactaatg gaaaacaaag gaattattcg taaccgaaga aaaatcgaag
     8941 cagccgtcca aaacgccaaa gcagtcttga aaattcaaga agaaatcggc agtttttcag
     9001 agtatctttg gtcgttttct aatggaaaaa tcgttgattc ttcttatcat tcacaagaag
     9061 aagtcccaac aacaagtcct ctttcagatg aattagcaaa agatctaaag aaaagaggat
     9121 ttaaatttat cggaagcaca acaatctatg cttttttaga agctgtcggg atcatcaatg
     9181 atcaccttga cagttgtttc cgcaaagaag aggtcgtacg tatcggcaga aagggcgaat
     9241 aaagcatgcg aaaaatcata ttttatggtg ctataagttt agatggctat ttggcaacga
     9301 aaacggatga tcttcaatgg ctttttgaca ctccaacagg taagcaaact acttatgaag
     9361 ctttttatga aacaatcgat acgaccatca tgggcagaaa aacttatcag gaagcaaaaa
     9421 aatatttgga gaccgaagca atttatccag agaaaaagaa tttcgttttt tccacccacg
     9481 cacaattaca attgccagat gctgaggtcg taaacgaaga tcctgttgct tttctaaaaa
     9541 atctacaaat gacagaagga aaagatatct gggttgtggg tggcggtcga ttgatcaaac
     9601 cgctattgga acatcaaatg attgatgaat ggtatatcca gattacccct gtattattag
     9661 gggacgggat tcctttattc caaaaaggaa catatgaagc gcgttttgaa atgctagaca
     9721 cgacacgatt cggtgaattt atcgaattgc attatgtacg aaagtagaag taaataaaat
     9781 aaaaaaggca actatatgca gaaatgtcct gtacatagtt gcctttttta ttaccgttat
     9841 gctttttact ctgttaattt gttagaaaaa tctagatacg catctaaaat cgtttggaag
     9901 aaacttaatg ttccttcaac gatattacca ttttcatcaa ttagattcgc aacgtttccg
     9961 atatatgctt ccggttgctg caaggttggc acattcaaaa agacaagtga ttggcgtaga
    10021 tggtggtttg cgccaaagcc gccgatccca cctggtgatg cagtaacgac taaacctggt
    10081 ttatgatccc aaacactgtg tccataaggt cttgagccta catctaaagc atttttcaat
    10141 actgctggca cagaacggtt gtattcagga gtaacgaaaa cgacaccgtc tagtcccctc
    10201 acttcctcac gaaaacgagt ccattcagca ggagcctgtt ctggagtttc gagatcctca
    10261 ttgtaaaatg gcagatcatc gatttttaca aatacacttt cgtacccttc aggaagcaat
    10321 ttcgcaaaag cttctgctac ttttttgttg taagaatctt ttcttaaact accaataaaa
    10381 aatccaattt tttttgtcat acgatttttc ctccttcaaa ctatctataa taatcttaca
    10441 aaagataagt aactggagca aataaaaaca cttactgaca aaagctacat agaagataga
    10501 ttacttattt ttaacaaaaa agaagtctaa aatcttctat tttctaaaaa aaatcgaatt
    10561 taatcaagct gtaaagaaaa aaagctttac agggtaaaaa aaaactgtta cactacatct
    10621 tgtgaataaa acaatggagg tggaaataaa taatggcagt taaaatccgt ttaaaacgta
    10681 tgggttctaa aaagagtccg ttttaccgta tcgtcgtagc tgattcacgt tctcctcgtg
    10741 atggacgttt catcgaaact gtaggtactt acaatccttt gaaagaccct gcagaagtag
    10801 ttttaaaaga agacttagtt cttgactggt tatcaaaagg tgcacaacct tctgacactg
    10861 tacgtaacat cctttcaaaa gaaggcgtta tgcaaaaaca ccacgaagct aaattctcaa
    10921 agaaataagg tgaccggata tgaaagattt aagcgactta gtcttaacaa tcgttcgtcc
    10981 gctagtcact tatcctgatc aagtcaagtt agacgtcgta gagtcgaaag acttttacga
    11041 gtacaatcta acagtggctc ctgaagatat tggccgtatc attggaaaac aagggcgtgt
    11101 tgctaaagca atccgcacaa tcgtttacgg tgttcggatc aatgcaccga aaaaagtgcg
    11161 tttgaatatc atcgatgata aggaaaagta aataaacgtg ctgtactcga attttcgagt
    11221 gcagcttttt tgtttttttt attaattgaa gaaatgatta tttggcaaaa caagcaagaa
    11281 agcgtatcct tataggagga gggagtgaac agccaatgag aaagaaaatg ttatttattc
    11341 catttgctct attgttaagc gcttgtagtt cggttactca agaatcagac aatacacatc
    11401 tatcaagcag tatatctgaa actgttacca gtccagaaaa aagtagtacc aaaacggctt
    11461 ccaccactga acaaacaatg gctacctcaa ataatgagaa aaagactgct ttagaccagt
    11521 tgaaagaaca acagccaaat gttcccatgc ctctagatgt accagtctct tcaggctatc
    11581 tcaatatcgc agcgacacat acaaagcaag gttattctat tttatattac agaacagatc
    11641 gtcccctcgg gttgaatgct gacgaattga atcaagaaac gcctattgca acctatctat
    11701 atcaatatgg gtttgcttct agtcaagaaa caatccaagt tttgcagcca tttgagatcg
    11761 acacaaatgg tcagcaagtg gacttaggtt ctcgaatcac agggtatcag caaggtgcag
    11821 caggttcaag ttttcttgaa tggcaagaag gaaactggcg tattcgaatc cgtgggaata
    11881 atatagaagg ccaagatccc ttactattag ctaaagaaat cgtagcttat ttggaagaaa
    11941 acagtctacc agcacctgaa caatttggta aaattactgt tgatatgggt gatacgacga
    12001 atcgagccgt agaagtaagc tggcaagaac cgaaaaatgc ctatacgatc actcatcaag
    12061 atcctatgtc tgcgttgaag atggcagttt ctatgaagag gttgtgaaat cagcgcgctg
    12121 atctgagccc atcatttaac tccaagtcac aggaagtaat caaaaatagc acgccgcatt
    12181 tttgcataat tttggcttaa taccttaaaa gaaaactaga attgcctctt cacacatgct
    12241 aaaataaaac agacaaaaaa gaaatgggga tataagttga ctgaatactt gaatgtagga
    12301 aaaatcgtaa acacacaagg tatcaaaggc gaagtccgtg ttatctcaac aacggatttc
    12361 ccagaagaac gttacaaaaa aggaacggtg ctaacactat tccaagacgg aaaagcacct
    12421 gtggaattaa cagtcaaaag tcatcgcaaa cataaaaatt ttgacttatt gagttttgaa
    12481 ggacatcctt ctatcaatga tgtggaaaaa tatcgggatg gcatattaaa agtgtcaaaa
    12541 gacaactctg tagatctagc tgaaaatgaa ttctattacc atgagatcat cggtctgcat
    12601 gtattagaag atggaactga gttaggtaaa gtaaaagaaa tcttgtcacc aggtgccaat
    12661 gatgtttggg tcgttcaacg tccgaaaaaa gctgatatat tgcttccata tatcgcttct
    12721 gtcgtcaaag aagtcgattt agaagctgga gtggtccgtg tagaaattcc agaaggactg
    12781 attgacgatg aagattgatg tattgacttt atttccaaga atgtttgaag gtcctatggg
    12841 ggaatccatc atcggtaagg ctgtagataa aggattatta gatatcaata tctctaactt
    12901 ccgcgattat tcagataaca aacatcagac agtagatgat tatccatacg gcggtggagc
    12961 aggtatgctg ctcaaagtcc agcccatcta tgataacatc caagccattg aaaaagctgc
    13021 accggatgtc aaaaaaagag taattcttct tgacccagct ggtaaacgat tcgatcagaa
    13081 aatggcagaa gaattttcac aagaagaaca cttagtattc atttgtggtc attatgaggg
    13141 atatgatgaa cgtatccgca cattagtgac agatgaagtt tccttaggag attatgtcct
    13201 tactggggga gaattaggtg cgatggtcat gatcgatgca acagttcggc ttttgccaga
    13261 tgtgttaggg aatcagactt cagctcaaac ggattcttat tcaaccggtt tattggaaca
    13321 tccccaatat actcgcccgg cagaatataa agggatgaaa gtaccagaag tattgacaaa
    13381 tggaaatcat aaattgattg aagaatggca attaaaagaa tcactaagaa ggacctatca
    13441 acgtcggcct gatcttttgg aaacacttga atggacacct caaatgacga aatttttaga
    13501 agaaatcaag aaagaagaac aagaaaaaac agcagatgaa gccaagtgac tttttctaaa
    13561 atcttcttta cagtaggttg ataatatgtt agtattttat ggtgagtgca aaagcactta
    13621 actattacga tattccgctg tgagaaaagc tcataagaat atttggaata aggagaagaa
    13681 aaccatgaat ccattaatcg aagaattaac aaaagaacaa ttacgttctg acatcccagc
    13741 gttccgccct ggtgacactg tgcgtgttca cgcaaaagtt gttgaaggta ctcgtgaacg
    13801 tatccagtta tttgaaggtg ttgttatcaa acgccgcggt gctggaatca gcgaaactta
    13861 tacagtacgt aaagtttcta acggagtggg cgttgaacgt acattcccat tgcatacacc
    13921 acgtgttgca aaaatcgaag tagttcgcta cggtaaagta cgtcgtgcaa aattgtacta
    13981 cctacgtgca ttacacggaa aagctgctcg tatcaaagaa atcagaagat aaacacggaa
    14041 aacttccttg gaaatctgaa atttgtgaaa agtccttgta tatcaagggc tttttctttt
    14101 ttctctatca cagttttaag ccatttttgg ctgttttgga ccatttttca atcatattcc
    14161 cttaaaaatt gtcctagatt gtccgacatt gactatcagt gtcgtcaaaa tagggcaaaa
    14221 tttagtagtc agcttgacga caaatataat acgaaaaata ccaaaacatt taccgaattt
    14281 agcaactttt gaacccaagt ttctcaaagg gtcagctatt agcaaaaaag acaaaacatg
    14341 tacttttcta ctaaaaagtt gatgtgatag gcttctatct aaataactat taggagtttt
    14401 tatcgtgaag caaaatgatg gacgtatcat ttggaaaaat caaagtgagt taaaactgat
    14461 tttaacaatt aatgaattca tcgaaaaaca cggaattaca tctagccgtc aataccagaa
    14521 aaagctatca gaaaatccaa attctgcacc aagtatgtgg ttcattaaca aaaaatatgg
    14581 atcatgggag aatttattga ttagtatagg acgagaaaat actgggtatg gaaaatgggc
    14641 tagaatgtca gaacaagaat tattggaaat agtagaagcc tttattaagt gcgagaagat
    14701 aacctctcaa cgaatgtacg aacaaaaatc agtagggaag aatattcctt cacttagtac
    14761 cattaaaaag atgttaggag atatacgtcc actttttaaa gaaaaaaatg atggatctcg
    14821 atttactgat ttcgaactgc tgttagaact aaaaaatgaa ataatccgat taaaactaca
    14881 ggatgattta tcaatgacaa agtttcgaaa actagttcaa tccccaaaac taccatcagt
    14941 tgatacaatt atgaaaagaa cgaacaaaaa ttgggaggaa ttgatggcag agattggatt
    15001 tgattatcga agaatcaaaa tttataagca gagaaacaac ctatccaaga caaagaaaac
    15061 taaatagtat cactctaaat cagacgttca aaattaaaat ttggacgtct tttttattac
    15121 ccaaaaacga aaggagattt attttatgaa aaagccaaga tttaagaatt ggcgtttgct
    15181 cgcaacactg agtctattat gccaaacaat tggtggatcg cttggcccaa cgattgcctt
    15241 tgctgatgaa attactcatc cacaaacggt aactgtggaa ttggatttgg ctcaccagta
    15301 tgctgtcgaa ggtacattta gcgatggtcg tcccatgtca gaagttaccg ttccgcacta
    15361 tgcagtttac aatggtgtga agcaagatat tttctgtatt gagccagggg tgccgattta
    15421 caatgagttc actcctggtt atgagaaaaa tccattgcct gatatgtcgg agaaagcgaa
    15481 attagtttcc gttttatgga aggaagctgg tacggatgtg gatacacata ttgtggccca
    15541 gaagatgatt tggcaagaag ttaatggcta cacgcttcac tcaattaaac gttctgatgg
    15601 tagtgcagta aatatcgcag ccattgagtc aaaaatcaat caagccattg cggattatca
    15661 aaagaaacca agcttccata actccacagt gaaaaccgtg ttaggtcaat cgactactgt
    15721 gacagatacg aacggcttga atttatcgga gtttgatgaa gttgtcgaga atatcgcaaa
    15781 tattgattat cgtgtgaacg gcaatcaact agtgattact ccaaatgcca attcaaaaga
    15841 gagtggtgaa ttaaccctaa agaagtcagc taatacagga acaccggtag cttataaaat
    15901 ggctggtcaa caaactttga tggcgggggc aattgataag cccaatacct acacaatcaa
    15961 aattgatgtg gaaactgagg gttctttgaa gattaaaaaa gtcgataaag aaacaggaaa
    16021 tgttgtaccg ggaaccgttt tccatttaga ctttggaaaa acattacctg caaaagacgt
    16081 gaccaccgat aaagaaggca ttgcgacatt ggatgagatt cctcatggcg cgaaagtaac
    16141 cattactgaa aaatcagtgc cagctcctta tacgattgac actacaccaa tcaccgcaac
    16201 cattaaatct ggtgagacta tttctgtgac ttcaaaaaat actcgcgaaa aaggtcaaat
    16261 cattttgaat aaaatggggg tggaaacagg aactgatctt tggaacgaca aatactcatt
    16321 agccggaaat acgtttgcca ttcgcaaaga cagtccaacc ggtgaaattg tccaagaaat
    16381 gaccacggat gaaaaaggtc atgcggaaac accaaaagag attgccaatg cgttggaatt
    16441 gggaacctat tatgtgaccg aaaccaaagc tagtaatggc tttgtgaata cctttaaacc
    16501 agtaaaagtc gaattgaact atgtcaatca aaccgtggct cttgttacca gtaacgtaaa
    16561 agggcaaaac caagaaatca ctggtgaaac cactttgaca aaagaagaca aagataccgg
    16621 taatgaaact caaggtaaag ctgagtttaa aggagctgaa tatactctct ttactgcaaa
    16681 agatggtcaa gcggtcaaat ggagtgaggc ttttaaacca gagttagtga agggaacgaa
    16741 agcttctgat gaaacggtga ctttgacttt agatgaaaag aatcaagttg ctgttaaaca
    16801 cttagcgatt aatgagtatt actggcaaga aaccaaagca cctgaaggat ataccttgga
    16861 tgaaacaaag taccctgttt ccatcaaaaa ggtggatgat aacgaaaaaa atgccgtgat
    16921 tacccgagat gttacggcaa aagaacaagt cattcgcttt ggctttgatt tctttaaatt
    16981 tgctggatca gctgctggga ctgccgaaac tggcttcaat gatctgacct ttaaagtatc
    17041 gccattggaa ggaaccagtg agattacagg tgccgaagat gaagcaatta cagcttataa
    17101 cgagcaacta ggttttgatg gctatggcaa gtttgaaaat cttccttatg gggattattt
    17161 acttgaagaa gtagaggctc cagaaggatt tcaaaaaatt acgccattag aaatccgttc
    17221 tacttttaaa gaaaataaag cggattacac taagagtgaa tacattttta cgattaccga
    17281 aaaaggacaa aaacaaccaa ttaaaacggt gaccgttcct tacgagaaac tgacgaataa
    17341 agcattttct gttagcttga atcgtttgat gctctatgat ttacctgaag aagaagatag
    17401 cttaacttct cttgggactt ggaaagacgg aggcaaagaa ttgacagacc ttgattctac
    17461 cgagctagtt gataaattga gctataactt gcatgaaatc aaagaagact ggtatgtggt
    17521 agcgcaagcc attgatgtgg aagcgacaaa agctgcccaa gaaaaagatg aaaaagccaa
    17581 accagtggta attgccgaaa caaccgcaac gttggcgaat aaagagaaaa caggaacttg
    17641 gaaaattttg cataaattaa ccgcagaaca agttttggat aaaaccatcg tcttattcaa
    17701 ctatgtgtat gaaaacaaga aaccctatga agcaggtgat gatccagtag cgaaagatgc
    17761 tagcttgaac aatcaagccc aaactgtcaa ttgtacggtg gaacgccatg tttccatcca
    17821 aacaaaagcc cacctagaag atggttcgca aacctttact catggggatg tggtggatat
    17881 gttcgatgat gtgtcgatta cccatgatgt actggatggc tcaaaagaag cttttgaaac
    17941 aattctgtat gcattacttc cggataatac gaccaaagaa atttggaaat ctggcaaaat
    18001 tgattatgaa gtgaatgaca aagaattcac taaaaccgta cttgcggaaa aagtggacac
    18061 aagtaaatat ccagaaggta caagttttac cttcaaagaa attaactatg gcaaagacgg
    18121 aaacatcaat gggaaacaca atgaggatct aaaagaaaaa actcaaactt taacacctaa
    18181 aaaagttccg acaacaccaa gtgcaccaga acaaccggaa ataccaactg ttccaagtga
    18241 ctctcaagaa tctagtccta cagtaaagac attcccacaa acaggggaga aaaattctaa
    18301 tgttctgctg tttattgggt ttaccttgat ctttgcgact gctgggtatt atttctggaa
    18361 tcgccgaaac taaggtgatg tgatgaaaca actaaatagg aaaaaccaac gtgcgcgacc
    18421 gcctcctaaa aaaagtggtc gcgctatttt tgtatccaaa actaaaaatg aaaagaggaa
    18481 aagaagatgg aattgaaatt tgttgtgcca gatatggccg aaacctttgg aaagataagg
    18541 tatgccggcg aaggcgaagt tttaactgaa ggatatgggc ggaatactac agtaattggt
    18601 cgtagttatc atttgtattc cagtaagcaa cgagccgatg atattgaagt ggtggtagct
    18661 gcggaagccg gcgaaaagga ttttgatcaa gatcaaccac taaaagccgt gaatccccat
    18721 ttggttgcca aaggctatga gattgagaat cgtgggttta ccgactacgt gttgtatgtc
    18781 gatgatttag tgaaagcata ggaggaaaag aagatgagat taacagaagg tattgttgta
    18841 gattcaggac taacgtttgg gaaattacga ttttccgcat tacgccgaga agtacggaag
    18901 caaaatgagg atggaacggt tagtaacgag gtaaaagaac ggacttataa tttgaaatcc
    18961 tctgcgcaag gtcgaatgat ccaagttagt atcccagcaa atgtaccctt acgcgagttt
    19021 gcctatgacg cagaggtaga gttggtcaat ccaatcatgg atacggtcgc caattatgtt
    19081 ttccgagaag gaactactgt caattggttc attaaggccg atgatttagt cttgaaacga
    19141 caaccagatc agggaaattc tacaaaccaa aatgaaggaa agaaataggt gaagcgtatg
    19201 gaaattaatg ttgaacatat tttagattgt ctcgatcaat atggaaaagg tgagctaacg
    19261 gaggaacaac tcactaaagc attaacctat gatgaaaaaa tgtttctgat tatgcatcaa
    19321 gggttactgg aagtaggtaa cgatgccgaa gaagattttg atgtactgaa ttggttaggg
    19381 gaagaagagt ccttctttat ggttatcgaa gtaaatgaaa gtctatgtcg agaagccgaa
    19441 accgtattgg aagaaattgg tgtggaaatg ccggatgcca ttgaagtttt tttaaagcaa
    19501 ctagtggaaa cgaaacaact ccctatagca gtaaatgact agcataactg cttgaaattg
    19561 gatcaatgaa atcctatgaa gcttttttcg attggaggtg gaaaccataa aaatgtacaa
    19621 agggcatcgt atacgggcag gagatcagca cttggtttat cactttgttc ttggctggct
    19681 actcgctctg ttcatcggtt ggatgagcgt cttttatttt caagagctta gacaatttga
    19741 tatttctaag ctatcacttt ctactataga aatcgttcgt tctataaaag atttaatcta
    19801 tttgctagga agtctcgttc tttcaggaag cacgatgttg ctttatatac acttttttca
    19861 ggatcattgg cgcagtttgt ggcatcgaca aaagctagcc cggatgattc tagaaaatca
    19921 ttggtacgaa gtaaaacaaa ctcagagtga aggatttttc aaagacctca atagtagtcg
    19981 aaccaaagag acaatcagtt acttccccaa aatctattac cgtatgaagg atggtttact
    20041 ttctattcgc gttcaaattt cactgggaaa atatcaagat cagcttttaa agctggaaaa
    20101 gaagttagaa agtggtctat attgtgaact agtagaaaag gaactcaaag actcctatgt
    20161 ggagtacact ttactgtatg atataattgc caatcgaatt gggatagacg aagtagtggc
    20221 agaaagtggg gctttgcgat tgatgaaaaa tcaagtatgg gcttatgatt ctttacctca
    20281 tatgttaatc gctggcggca caggtggtgg gaagacttac tttttactca ctatcatcga
    20341 ggctttatta aaatctgatg ccgagctttt tgttcttgat cccaaaaatg cggacttagc
    20401 cgatttaggt acggtaatgc ctcatgtcta ttcccaaaag gaggaaattt ctgcttgtgt
    20461 ggaagatttc tatgagcgca tgatgactcg tagcaaggca atgaaagaaa tgcccaacta
    20521 caaaacagga gagaattatg cgtttcttgg acttccacca aactttttaa tctttgatga
    20581 atacgtggtt tatatggaaa tgttaacgac gaaggaaagt gcggtgattt tgaataagct
    20641 caaacaaatc gtgatgttag gtcgtcaatc tggtttcttt ctgattctag cttgtcaaag
    20701 accggatgcg aaatatttag gagacgggat tcgcgatcaa tttaatttcc gggtggcttt
    20761 gggtcgtatg agtgaactgg gatattccat gatgtttggc gaggttgata aaaaattctt
    20821 tatgaagcgt atcaaaggtc gaggctatgt agatacggga gggagtgtga ttagtgagtt
    20881 ttatacgcca cttgtaccaa aaggatatga tttcttaagg gaaattagcg acattggtgg
    20941 attaaacgtt aatacggaag gataaaataa tagtatgtag cctaggagtg tcaagaatcc
    21001 tgggcttttt ttacgaggtg atttatgaca tttttagatt tattcgctgg gattggtggc
    21061 ttttgcttag gtatggaaca agcgggtcac cagtgtatcg gcttttgtga aattgatgat
    21121 tttgctcgcc agagttataa ggcaatccat gatacaagta aggaggtgga aatgcatgac
    21181 atcacaagtg tatcagacga gtttattcaa tctctcggac cagtggatat cctttgcggt
    21241 ggatttccgt gccaagcttt ttcaattgcg ggaaagcggc aaggattttc cgatactcga
    21301 ggtactttat tctttgaaat cgctcggttc gccgctattc tccaacctaa gtttttattc
    21361 cttgagaacg tccggggatt actcaatcac gaaggagggg ctacgttcga gacgatcctc
    21421 cgaacgctgg atggattggg gtatgatgtg gaatggcaag tgcttaactc aaaggcctac
    21481 gttccccaaa accgtgagcg gatcttcctt atcggacatt ctcgagacgc atgtaccgaa
    21541 caagtatttc ctatcattgg atcgtctcca acatctgatc aaaacatcag aaacttatta
    21601 aatatcaacc cttccaatcg aggaatgggg ggacaagtct atgggtcaga tgacgtagcg
    21661 cctaccttga ccggtgatga aggaatcaaa atcgcattgc cggtagatga tggaatttct
    21721 gtagcgggta tgttactggg aaattttgaa caaggcaatc gggtttatga gataactgga
    21781 acagcgccta ctttgtcaac gaaacaaggc ggaacgaaga tcatgcttcg gaaaaacgaa
    21841 acagaatatc aagaggtgaa gccagggaac agtgtgaacc tggcttttcc taattccaca
    21901 agtcgtcgag gccgtctggg aaaacaaagt gtgcatacct tattaacagg agaccaacaa
    21961 gcagtcgtca cggatcagta tcaaattcga aagttaacac ctagggagtg ctggcggctt
    22021 cagggctttc cggactgggc ttttgatcga gcttctcaag tcaattcaga tagtcagttg
    22081 tataagcagg ctggaaattc tgtaacagtt ccggtcattt ttgatattgc tagaagatta
    22141 aaggaggtaa aaacagatga tttatgatga gttgattggg gaatctactg ggtgattggc
    22201 aagattcagt cagatccgga attggaagaa gaactgtacc ggctcaattt tgatattcgc
    22261 aaaaacgggg tgaaagtccc tgatgatccg tatgtaattg atgaggaaac ggatgctcga
    22321 atggagctga atcaagtcat cagtgaattg gaacgaattg cggactttgc taaagaacct
    22381 gatatccgcc aatacttgtt tgagataaaa gcagagttgg aaattgaggg tattaccgcg
    22441 gagtaacggc caaataaacc actgataggc actgaccgcg ggggatttgc gaagcagaaa
    22501 tcctccgtgg tgctgtgcct gtttatggac ggcgttagcc ggccatgatg tctcaccccc
    22561 cgtatctaac aggggggtac aaaaacacaa aaaataagca agcaacgata aggaatggct
    22621 gtcctatcaa ggataataag caatttgtgt agatgttaac tgatttgaaa aagttaacac
    22681 cttaaatttg gaggtgagaa cttggcacaa aggaatttag attatcggct attaaaagac
    22741 cggcgtaatg aatatggcgt ttcacaaaat aaattagcta cgacttgtgg acttagtcgc
    22801 ccgtacttaa atcagataga aaatggtggc gtaaccgcat cgacaaaaac catgaggaaa
    22861 atttttgacc aactggaaag tttcaatccc gagttacctt taacgttact gtttgactat
    22921 gtaaggattc gctttcccac aacggatgcg aggaaaatca ttcaagagat tctccatttg
    22981 aaatttgatt atatgctcca cgaagattac gccttttact cctatcaaga acaatatgtt
    23041 atgggagata ttgtggtgat gttgtcacat gaagaagata aaggcgtcct tttagaatta
    23101 aagggtcgtg gttgtcggca gtttgaaact tttttactcg cccaaaagcg tagctggtat
    23161 gactttttcg aagattgtct aaaagccggt ggcgtgatga aacgcttaga cctggcaatc
    23221 aatgatcgag ttggcctgtt agatattcct gatttaacga aaaaatgtca gaaggaagaa
    23281 tgtatctcct tgtttcgtac cttcaaaagc tatcgttccg gggaactgtt gaaacctgat
    23341 gataaagatg gtatgggcaa tactttgtat attggcagtc ttaagagtga ggtctatttc
    23401 tgtttgtacg aaaaagacta tgagcaatat atcaagttag ggattccttt agaccagacc
    23461 gaaacgaaaa atcgttttga aatccgactg aaaaatgatc gagcttatca tgcaattcaa
    23521 gatttgttaa aaggtcgcag tattgaaagt accacatttt ccattattaa ccggtacttg
    23581 cgatttgcgg ataaggtgga aggcaaaagg cgaactaact ggcccttgaa tgaacaatgg
    23641 gggcgcttca ttggacggaa tcagaaggaa attcaattaa cttctgaacc gaagccttat
    23701 accattgaac gaacgttgaa ctggcttgga cgacaagtcg cccctacgtg gaagatggcc
    23761 aaggagttgg atcggttgaa tcagacaacg tacattcagg atatggtaaa taatactcga
    23821 ctatcagacc ggcacaaaaa gattttggag caacaaagta tggcaattga aaatttgatt
    23881 atatgaaaag aggaatcgtg atggaaattg tgaaagatat tttattggtg tttggtggca
    23941 gtatgcttgg tgttactgtg atgtgcttga tgcatgcaag tgcagcggcg gaccgagcaa
    24001 tggaaaagaa aggaaagtga tgaaatgaat ttcggtcaaa atttgtgtgc gactcgttcc
    24061 ttagtgaaaa gctaaggcac taataaaaga acgaaaaagt ttggttatta ggagaactga
    24121 taatctgaca ggtggaatga tgaagtaacg ctctgaaacg cctgccctga gcctccgaca
    24181 agcattgtat gaggaaactc agaatgtttg aagctcggta aagacggctg aaagataccg
    24241 taatagttga tgaacatata tcttctatcg aaaactatcc agaggtgggt agcgtatcca
    24301 acaggtcggg ggtctataaa atatctatgg ttagaatgtt ctataaggaa ctgacgaaac
    24361 agcgaatgta cgggtctaaa tttgaacttt gtagaaatgc aaaggtacga gaaatcgtag
    24421 ctggtgtggc aaagtaagaa tataggttat gaaatgccat ataacgttac aggcggtatc
    24481 aagccagcag gcttatagct agtgacctaa ggatatatat gtatagataa gattatcgga
    24541 acgaggaaag gtattgggtt ctcatcaaga gaataaactg acgaagaaca ataagcagtt
    24601 gaactaatgc tgaaaagcag agggcgaacc taagaaattc ttgtaatgag aatggaggaa
    24661 tgccctcaag tcgtgtatat attagaaaag tattatgttg tttcaataag gattacgagt
    24721 aagataaaaa agctcactca atttcaagta aaggagtgaa gccttatgcc taaaaataaa
    24781 aatgaaacat tgtgtgtaga agatttaaga cacgctgaat attacgaaat gcaaaacacc
    24841 tttgatgatt tatatgccaa aagtaagaat ggagatattt tcactcatct tatggatatt
    24901 atcctatcga gagaaaatat attactggca tatcgaaata tcaaagcaaa tgcaggcagt
    24961 aaaacagcag gaacagacgg tacaataatt aaagatattg gcaaattgcc agcagaaaca
    25021 gttgtcaaaa aagtaagata cattgttgca ggcagtccac atgggtatcg acctaagcca
    25081 gtgaggcgaa aagaaatacc aaaaccaaat ggtaaaacca gacctctagg tattccttgt
    25141 atgtgggaca ggcttataca acaatgtata aaacaagtat tagaaccaat ctgcgaagct
    25201 aaatttagtg aaaacagcta tggttttaga cctaataggt cagttgaaaa tgcaataaaa
    25261 gcaacctata accgcctaca aatcagccag ctacattatg tgattgaatt tgatattaag
    25321 ggtttctttg acaacgtgaa ccattccaag ctgataaaac agatatgggc tatgggaata
    25381 cgggacaagc acttgatatt cattttaaaa aggatattaa aagctccaat caaaatgact
    25441 aacggtacaa taacatatcc agagaaagga actccgcaag ggggaataat atccccacta
    25501 ctagcaaaca ttgttcttaa tgagttagac cattgggtag aaagccaatg gcaggaaaat
    25561 cctgttacaa aaaactatgt ggttcatatc aataaaagcg gtagcccatg taaaagcaac
    25621 gcttacaaag aaatgaagaa aacaaagtta aaggaaatgt atatggttcg ttatgcagat
    25681 gattttagag ttttctgccg ctacaaggaa agtgctgaaa aagccaaaat agcaatcact
    25741 caatggattg aacaacgatt aaaattggaa gtatcacaag aaaaaacaag aattgtaaat
    25801 gttagaaaaa gatactccga ctttcttggt ttcaagatta aaatgatacc gagaagaaag
    25861 aagttagttg ttaaatcaca tataagtgat aaacaattca aaaaccaaaa agacaagctg
    25921 gtatcacagg caaagaaaat tgcttctccc tcaaaaggga aaacggaatt agaagaaaca
    25981 agactttata attcaatggt attaggtatg caaaattatt atcagattgc aacgaatgta
    26041 aatctggatt gtatgaaact gaatagagct gtaatggcta tcttcactaa cagattaggt
    26101 acaaacaaaa ggggcagatt gagaaaaaca ggcagacaac ttacgacaaa ggaggcaaca
    26161 agatatggta aatctaaaat gcttcggtat gttgctggcg agggcgaacc tattttccct
    26221 gttggatata tccaacacag aaaaccaatg gcaaaggtgt atttagcgaa ttgctacact
    26281 ctagagggaa gaaaatttat ccacaccaat ctaagtgttg ataaattact gatgtaccag
    26341 ttaatgaaat tatctttgga aaatcgcaca atagaatacg cagacaatcg aatttcgcta
    26401 ttttctgctc aacatggaaa atgtgctata actctggaag aatttcaaca agctaatgaa
    26461 atacattgtc atcatattgt accaactgga gctggtggaa atgacgatta taaaaatctt
    26521 attttggtaa aagaggcggt acaccgtcta atacacgcta aaacagaaga aacaatacaa
    26581 aaatatatag ctctattgaa gctaaataaa gtccagctca taagactaaa taaatataga
    26641 gtattagctg gcaatcaaga attaaacttg atataggtct actacataaa tacttttcaa
    26701 tataaacacg atggaacgcg gagtgcggtg aaagtcgcac gctccgtgtg aatgggggga
    26761 aaagtttgag atatttataa tatcaggaac ttacctatcc atatatcaat ggtttttaac
    26821 caatgctcaa tctttagtct tactagcgat tgtggtcatt ggtttgtttt taggatttaa
    26881 acgcgagttt tccaaactga ttggcttctt ggtgattgct ttgattgcgg taggtctggt
    26941 ctttaatgct tctggtgtga aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc
    27001 ctgaggggga atcacaatgg aacagatacg tgtttatatt gcgaaccttg gaaaatacaa
    27061 tgaaggtgag ttggtggggg catggtttac cccacccgtc gattttgatg aagtgaaaga
    27121 acggattgga ttaaatgacg aatatgaaga gtacgcaatt catgattatg aactgccttt
    27181 tgagattgat gagtatacac caattgagga aattaatcgg ttgtgtggtt tagcggaaga
    27241 gcttaaagga acgccaattg gtgaggtcgc ttcagagatt caacatgcct tcttcaattc
    27301 tttcgaggaa atggtagagc atgtggatga tatcatctat tatcccgatt gtaatgacat
    27361 gagtgatgta gcgtattact tgattgtaga agctggtgat ttaggcgaag taccagaaca
    27421 cttgaagacg tatattgatt ttgatgcgtg tggtcgagac ttggagattg gcgggaatta
    27481 tttggttacg agtcgagggg tttttgaact aatgtaaatc atttgttaga attgaccaga
    27541 aatttctatc gtacttttac ttttctattt gatatactga aatagaaaat atttgttgat
    27601 aggggtgtta ctaatggttg gcaataagtg gtacaaatgt gatcttcata ttcattcaac
    27661 tgcgagtcag tgctttcagg ataaaaaggt tactgctaag gaatgggtag aagaatgttt
    27721 aaacaaagga ctcgattgtg ttgcattaac tgatcacaat acaggggaaa atattgatga
    27781 atataagaaa gtggctatcg aaaatggctt agattttttt cctggagtgg aattaacgtg
    27841 tggagaaaat ggaactcatt tactagtcct atttgataaa agtgcatgta caaatgatat
    27901 ctctgatttc ttaaccaaga tgggtataaa aagagaggat tgggccagcg ctgcagcttg
    27961 ctcaatgaaa tcagttttag aagttattga ttttgctgag cacgagggct tgctcgttat
    28021 tcctgcacat atagatgagt ttaatggaat ttcttcagca aaatttggaa atctaggaaa
    28081 gatatttgaa tcagaaaata tatatgcagt acagacggtt caaaaagaat tttttgaaaa
    28141 tcgctcacaa atgattccct catctaagcg tgacactatc tatgatagtg tgaatgccag
    28201 atatgatggt agagttggaa aagatacttt ggaatcttgg tataaatcag ttgttgaggc
    28261 agaaaaaaat aatatgacat ttcttagctt ttctgataac ccacactcga aaggaaattc
    28321 aaaacatgga ctttggggaa taggcacaag atattcctat ataaaaatga aggacgaacc
    28381 tgacttggga tcacttcgcg atgctttaat gctaggcgaa actcgagttc attctgattt
    28441 ttctaatttc tccattaatg aaaatgaagt tttattggaa aaattaagct tttctgggac
    28501 cactcttagc acaaaagaag ttgtagtcga attcagcgat aatttaactt caattattgg
    28561 aggaagagga acaggcaaat cttgtattac aagattcttg gtctatgtcc ttggaaagga
    28621 agcagaactt gatcaatttt ctgaaataca aagtgattat cagaactttg cacaaattga
    28681 acataatggc tctggaatat ttttgaagga tacaattgtg aaactgaata ttttctataa
    28741 ggggacaaaa tatcaaataa taagaacaca ggatagacat tctatttatg aatttacaca
    28801 agagaatggt ctggttgaaa aagaaacaga acggttgaga atgatttccg ataaggtgag
    28861 tatatactct caaaaacaaa tttatgaaat ttcaaaaaat caaactagta tactagaatt
    28921 actagatgga tataactctg atttgatttc agagtataaa aatgagattg aaacatgtgt
    28981 aaacgaaata agaaaattaa attgggatat tgtttctgtg aaaaaagaaa tacaagataa
    29041 ggcgaaggta gagcttgaaa ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa
    29101 gagttataaa gatgtttatg atgagtattc aaaagagaca gatatttata gggaactgaa
    29161 acgtgatgtg gaggcactta aggaaattcc caaaaatcta actgatagtg ttgacaaaat
    29221 tgactttggc aatggtataa aagttacaga cgaaattgat gaacaccgcg gagaattgat
    29281 taagaattta gtgaccaatc gggctaagat tcaaacaata attaatgaaa tgtcagatga
    29341 aattgatagt tatagagcag agctgaataa atcagattgg aaagttaact ataatgaggt
    29401 ttcaaagagg tatcaaagag gtatcaaaaa cttagtgaag agttatctca agatgaatta
    29461 aaaatgttac aaaaccttga ttctttatat caatctttat caaaaactga agagcgattg
    29521 gaagaaatta ttcaacaaga aataaagctt aaagattacc aagttgaggt tgactcagca
    29581 atgtcaagaa tgaataaatc atatcaaaat ctctattctg agagaagtag ttcctcgcgc
    29641 gaaatttttt ctgatattac aggaatagag gctactattc aaccaaatag ggatttcaat
    29701 aattatgtta aagagctcag agcttgtatt aataaggagg acacttttga cagtgagttt
    29761 ggatctctta ttgataagct gacaaaaaat tccattacta atcaagatat atataatgac
    29821 atacttgata taagagttag tggggaaagt agtatttttt cagataaaaa attaatcaat
    29881 tctataagaa aattgaatga tgcacaagtt cttgaattac aaacccttaa gccaaatgat
    29941 ttagtagtta tccgacttga actaaatgga aaaaaagtta gtttatcgaa tgctagtgct
    30001 ggtcaaaaga catctgcaat tctttctatg attttggcgt atggaaattc accactcata
    30061 ttggatcagc ctgaagatga tttggatagt caattaatta ataacttaat cgtaagaagt
    30121 atcattacaa aaaaggaaaa cagacaaatt ttaattgtaa ctcataatgc aaatatccca
    30181 gttaatggtg attcagaatg gttgattagc atgggagatt caaaagaaat atcggctgat
    30241 cagtgcgggt cggtagatga acccaatatc aagccaagaa tatgtactgt gatggaaggt
    30301 ggcgaggatg cttttacaaa tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa
    30361 cactagaggg tgagacatat tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat
    30421 aaaaagctat accagtatct gggcagttga aaaagtcatt tacgcaatca atgattttca
    30481 gttgcctttt cctgtgactt ttaaccaaat ggcttggttc gttttatctt tactgtttgt
    30541 catcgtattt gcccatgtgc ctccactatc tatgatagag gaggtctttt taaaatactt
    30601 aggcataccg gtggctgtca cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc
    30661 actgggattt ttacgatcct ttatcagcta tcacttgcga tttaaggtga cttttgcagg
    30721 aaagaaagtc aaagaacaga agaaacgatg ggatgaaccc agtactcttg taaggagtgt
    30781 gaactatgta tccgattaag tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt
    30841 gttttgccta ctatgaatta gtgccttaca attactcctt tttgtcaccc gaacagaaat
    30901 ttcaagtgca tgataacttt cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg
    30961 cgttacaaat tgccacagaa agtagtattc gcgccacaca agaacgatca aaaaaagaaa
    31021 ttaccggtag actcaaagaa gtagcgaaac aacgaattga tatacagaca gatgcattag
    31081 tatccatgat tggcgatagt cagattgatt atcgcttttt tattggcttt aaactaattg
    31141 cgacagatga agaagtcaat ctaaagagtc tcaagaaatc tttcttttct gggcttcaag
    31201 aatttgtcta tggagtgaat catcatttga tgggcgattt tgtttcctta tccaatgaag
    31261 aaattcgctg ctattctaag cttgaaaaat taatggaaag caaattggct cgaagattta
    31321 aagtcaggcg agtcacaccg agtgacctga cgtatttgat tgaacatatt tatggcgaaa
    31381 aagggatacc gtttgaagaa tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa
    31441 cactggtgaa acgttatgac ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa
    31501 ggtgcttacg gatggaacat gagaatcatg agtcgtacgt agcgtattta acaattaata
    31561 caattgtggg ggagatggaa tttccttcct cagaactttt ctattaccag caacaacaat
    31621 ttacattccc aatcgacaca agtatgaatg tagagattgt gacgaataaa aaggctttag
    31681 ctaccgttcg taataagaaa aaagaactga aagatttaga caatcatgct taccaatctg
    31741 acaatgaaac gaattccaat gtcttagatg ctttggattc cgtggatgaa ttggaaacca
    31801 ccttggatca gtcaaaagaa tctatgtaca aactgagtta tgtggttcga gtgagtgccg
    31861 aatcagtgga tgaactaaag cgacgctgtg atgaagtgct cgatttttat gatgatacca
    31921 atgtaaaact agtccggccc tttggcgata tgatggggct acatgaagaa ttcttaccct
    31981 caagtaaacg atatatgaac gactatattc agtatgttac gtctgatttt ttggcggggc
    32041 ttggttttgg ggctactcag atgctaggag aattagaagg gatttacttt ggctacaatg
    32101 tggatacagg tcgtaatgtt tacctgaaac cggcattggc ttcacaagga gtcaagggtt
    32161 cagtaaccaa tgcgctggcc gccgctttcc tgggttctct tggtggaggt aaatccttta
    32221 gtaacaatct cttagtctac tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc
    32281 caaaagggga gcgaggaggg tggaaagaaa ccttgccaga aattgcggat gaaatcagta
    32341 tcctcaactt gacgagtaag tcagaaaatc aaggtctgct tgatccttat gtgattatga
    32401 agaaaaagaa agactcggaa agtttagcaa ttgatattct gactttttta acaggaattt
    32461 caagtcgaga cggggagaag tttcctgtgt tacgtaaagc cattcgaacg gtgactcaaa
    32521 gtgatcaacg ggggttatta caagttatta acgaattgcg aaaagacggg tcaccaatcg
    32581 cggaaaatat cgccgatcat atcgagtcct ttaccgatta tgattttgct catttgcttt
    32641 tctcagacgg ctcgattacc caatcgatta gtttagataa acagctaaat atcattcaag
    32701 tggcggattt ggttttaccg gatgcagaaa cgacttttga agaatatacc accatggaat
    32761 tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc cttggacttt atccatagta
    32821 accgagaaat cttcaaaatt gtcgatttgg atgaggcttg gagttttctt caagtggctc
    32881 aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg ggccatgaat gctggggtct
    32941 atttcgtcac tcaaaatagc gacgatttat tagatgaacg tttaaaaaat aatatcgggt
    33001 tgaagtttgc ttttcgcagt accgatattc atgaaatcaa gaaaaccttg gaattctttg
    33061 gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg agatttggaa aacg
//
LOCUS       DACEOM010000030        31983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEOM010000030 DACEOM010000000
VERSION     DACEOM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.031"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..379
                     /locus_tag="IW781_001330"
     CDS             <1..379
                     /locus_tag="IW781_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1182334.1"
                     /translation="EVDPEEKPDVTPEPDTDARDQGIPEKINKKTIQEDGKKESKKSN
                     LAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQLPKSGESQNKVILW
                     SGIILLSIATMLSAKRFKQNRSL"
     gene            complement(505..1584)
                     /locus_tag="IW781_001331"
     CDS             complement(505..1584)
                     /locus_tag="IW781_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1182335.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1693..2133)
                     /locus_tag="IW781_001332"
     CDS             complement(1693..2133)
                     /locus_tag="IW781_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1182336.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2190..2600)
                     /locus_tag="IW781_001333"
     CDS             complement(2190..2600)
                     /locus_tag="IW781_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1182337.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2781..3215)
                     /locus_tag="IW781_001334"
     CDS             complement(2781..3215)
                     /locus_tag="IW781_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1182338.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(3315..4163)
                     /locus_tag="IW781_001335"
     CDS             complement(3315..4163)
                     /locus_tag="IW781_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1182339.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4552..6318
                     /locus_tag="IW781_001336"
     CDS             4552..6318
                     /locus_tag="IW781_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1182340.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6420..7679
                     /locus_tag="IW781_001337"
     CDS             6420..7679
                     /locus_tag="IW781_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1182341.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7826..9127
                     /locus_tag="IW781_001338"
     CDS             7826..9127
                     /locus_tag="IW781_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1182342.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9130..9981
                     /locus_tag="IW781_001339"
     CDS             9130..9981
                     /locus_tag="IW781_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1182343.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9994..10809
                     /locus_tag="IW781_001340"
     CDS             9994..10809
                     /locus_tag="IW781_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1182344.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10960..12126
                     /locus_tag="IW781_001341"
     CDS             10960..12126
                     /locus_tag="IW781_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1182345.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12356..13003
                     /locus_tag="IW781_001342"
     CDS             12356..13003
                     /locus_tag="IW781_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1182346.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13087..14031
                     /locus_tag="IW781_001343"
     CDS             13087..14031
                     /locus_tag="IW781_001343"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1182347.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14166..15617
                     /locus_tag="IW781_001344"
     CDS             14166..15617
                     /locus_tag="IW781_001344"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1182348.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15638..16474
                     /locus_tag="IW781_001345"
     CDS             15638..16474
                     /locus_tag="IW781_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1182349.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16474..17133
                     /locus_tag="IW781_001346"
     CDS             16474..17133
                     /locus_tag="IW781_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1182350.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            17259..17948
                     /locus_tag="IW781_001347"
     CDS             17259..17948
                     /locus_tag="IW781_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182351.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            17954..18910
                     /locus_tag="IW781_001348"
     CDS             17954..18910
                     /locus_tag="IW781_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1182352.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            18954..19466
                     /locus_tag="IW781_001349"
     CDS             18954..19466
                     /locus_tag="IW781_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1182353.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19722..20270)
                     /locus_tag="IW781_001350"
     CDS             complement(19722..20270)
                     /locus_tag="IW781_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1182354.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(20359..21540)
                     /locus_tag="IW781_001351"
     CDS             complement(20359..21540)
                     /locus_tag="IW781_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1182355.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(21776..23284)
                     /locus_tag="IW781_001352"
     CDS             complement(21776..23284)
                     /locus_tag="IW781_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1182356.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            23443..23634
                     /locus_tag="IW781_001353"
     CDS             23443..23634
                     /locus_tag="IW781_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182357.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            23837..24754
                     /locus_tag="IW781_001354"
     CDS             23837..24754
                     /locus_tag="IW781_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1182358.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            24747..25820
                     /locus_tag="IW781_001355"
     CDS             24747..25820
                     /locus_tag="IW781_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182359.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            25968..26486
                     /locus_tag="IW781_001356"
     CDS             25968..26486
                     /locus_tag="IW781_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182360.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            26467..26859
                     /locus_tag="IW781_001357"
     CDS             26467..26859
                     /locus_tag="IW781_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1182361.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            26935..27870
                     /locus_tag="IW781_001358"
     CDS             26935..27870
                     /locus_tag="IW781_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1182362.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28003..28782
                     /locus_tag="IW781_001359"
     CDS             28003..28782
                     /locus_tag="IW781_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1182363.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            28775..29092
                     /locus_tag="IW781_001360"
     CDS             28775..29092
                     /locus_tag="IW781_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1182364.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            29336..30946
                     /locus_tag="IW781_001361"
     CDS             29336..30946
                     /locus_tag="IW781_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1182365.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31103..31951
                     /locus_tag="IW781_001362"
     CDS             31103..31951
                     /locus_tag="IW781_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1182366.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        10512 a         5693 c         6256 g         9522 t
ORIGIN      
        1 agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga
       61 ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag
      121 taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg
      181 aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc
      241 tagtcctgaa acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat
      301 actgtggagc ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa
      361 acaaaacaga tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag
      421 aaatatccag aaaacgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat
      481 actttatctt tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg
      541 gatctttttc actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa
      601 gccccaaacg ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg
      661 cagttctgtt ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac
      721 aatcaaaatc gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat
      781 gaaagataga ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa
      841 aatcatgatc gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt
      901 gagcaacgtg tttaaaagag cgataacaaa ttgagcttct aataccaaac caaatgtatt
      961 gacaaactta tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa
     1021 aaacaatctt gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat
     1081 gaatgacata gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat
     1141 cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata
     1201 ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa aaatcaataa cggactggat
     1261 catatcaaat gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag
     1321 gtaaacgatc agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg
     1381 ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt
     1441 tgtaatcata cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg
     1501 gcgtattttt tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata
     1561 acaaaaaata aacacgttat tcattatatg atggaatatg aaaataagaa agagaaagag
     1621 caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt
     1681 catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt
     1741 cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg
     1801 tggaatacct tctgcttgtt tttgcaacgc agcaccttga ggagtcagcg taacatttac
     1861 gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt
     1921 tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga
     1981 agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag
     2041 cggcttgagg agggaagtgt agtgtctaat gatttcctta gaggcggcat ataaaggaaa
     2101 acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag
     2161 tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct
     2221 atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct
     2281 tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag
     2341 ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata
     2401 tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca
     2461 aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat
     2521 tcgaatgtac gatcgggatt atgaacttct ccagagcgtc cgcctgtgtt gatcattttc
     2581 gtagaataca tttttttcat aaaattacca cctttaatta tattttatac aattgaattt
     2641 tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc
     2701 acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaaatat cagagacaaa
     2761 aactaatctc atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa
     2821 cttacttcac gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt
     2881 gagttagtca caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt
     2941 cgtaatgctt tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta
     3001 tttggatgtt cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg
     3061 aatctcgtgc catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc
     3121 tcaatcagtt gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca
     3181 tttaatacaa aatctatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg
     3241 aacagttact agattctttt attataccag ttaaagaaaa cgcattcaaa aaaatagcgg
     3301 atttacttac caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct
     3361 acgctgctga caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg
     3421 gaaaattcat gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg
     3481 ccttttgcct ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg
     3541 tcttccaaat aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg
     3601 atataagtca tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct
     3661 attccaaaaa caaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc
     3721 gaagtattca agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta
     3781 gaagagttgt ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg
     3841 acagacaaca accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga
     3901 aaaaacaagc ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct
     3961 agtaccaaca taacgatatt caacactaaa ataactatag ctcggtcaaa tcctaaaaca
     4021 gcttccagta atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa
     4081 aacatattga tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat
     4141 ataaattgtc tcgtttcttt catttatcca cttccttaat tcattaataa tactcttatc
     4201 ttattataac ttacttttca ccttaagtta actttaatta ataaaaaaat taggctagtc
     4261 taaattatta tgtttcattc ttctagaata tacttctacg ttaaaaactg ttaaaaaact
     4321 tttactttag aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta
     4381 caggaagtag attaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa
     4441 taaaaattcg gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgtt
     4501 tctctatcac ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca
     4561 gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt
     4621 cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct ttgtggagat
     4681 ccatatacca taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta
     4741 agtaccaatg ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag gcgcttacag
     4801 tatgcgttcc atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt
     4861 tttccttatc gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt
     4921 catcaaatcg atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt
     4981 tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg
     5041 ggaagtaaag atccagaccg agaagacttc ttcgggggag atttgcaagg tgtgagggat
     5101 catttggatt acttgacgga ccttggcgtc aatggcatct atttttgtcc catatttaaa
     5161 gccacatcca atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc
     5221 gataaaaaac ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg
     5281 ttcgatgctg tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa
     5341 aacggagaaa aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct
     5401 tatcagatga ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt
     5461 actccattca tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat
     5521 atcgcaactt attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat
     5581 gaagtcgatc atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac
     5641 atctatatcc ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc
     5701 catgcagtga tgaattatgc attcacagat tcgattaaag attattttgc aaaaaagaaa
     5761 atcacagctt cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag
     5821 gtgaacgaag gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt
     5881 tgccaaggta acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg
     5941 tctccttgta tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc
     6001 cgcaaatgta tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa
     6061 gaacttgttt ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg
     6121 ctaatagtat atgatcgtga agataagctc tatttcacac gggaactaga gggacagatc
     6181 atctatgttt actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg
     6241 attcttagtc aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga
     6301 tttttgatca tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag
     6361 atttgtgccc attataataa gggtgtaaca gcacgttgaa tacacaggag gaaatgaaga
     6421 tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg
     6481 gattagcggc ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa
     6541 cgttaacagt ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt
     6601 tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg
     6661 aatcattagc gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg
     6721 accggatcgg tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag
     6781 cagcttacga tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac
     6841 ctgcagtaat cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat
     6901 caacgtttaa agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag
     6961 caggaaaaaa tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa
     7021 ttgcaggtta cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt
     7081 tgaataatgc aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt
     7141 ggccaaaggg aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt
     7201 ccaataaaac tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca
     7261 atatcaatta tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt
     7321 ttggcggagg aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa
     7381 aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg
     7441 aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa
     7501 cagctgtgat tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg
     7561 aagtttggac aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa
     7621 aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat
     7681 taaaatgata accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca
     7741 agagcgagat ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt
     7801 tagcaagttt agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa
     7861 aaaaatcacg ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt
     7921 taaaggtatc gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg
     7981 atttcatgca ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt
     8041 atttattttg attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta
     8101 cggtttgaat cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat
     8161 caatcggacg atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt
     8221 gacacttccc gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt
     8281 gttcactgca tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg
     8341 ggtaggggct aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt
     8401 tgcagtcttt tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct
     8461 tggtattctc acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg
     8521 tatcattttc ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa
     8581 tatattcaac gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct
     8641 tccttttgta gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc
     8701 aattatctta gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat
     8761 cttacaatcg atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg
     8821 gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat
     8881 tacacagtat acggggaatt tcaataattt ctccatgatc tatctgttta acaatggtgg
     8941 tcccggaagc gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa
     9001 acttacgact ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc
     9061 cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg
     9121 ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt
     9181 tttacttact tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca
     9241 gcaagtactg ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac
     9301 tggactttag gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa
     9361 aatacattga tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg
     9421 ggctatactt atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta
     9481 gtgattcaga tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta
     9541 ttagaagcgc tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca
     9601 atgaatactt ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct
     9661 gctaaacttg atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc
     9721 aaaccgatgc tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg
     9781 ttagctaagt tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca
     9841 tttatttctg atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt
     9901 gcactaccga tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca
     9961 tctggcggaa caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt
    10021 tccgcattac gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa
    10081 tttttcttga tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat
    10141 gccaatcaga tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat
    10201 gaacaaggaa cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg
    10261 caacaaacga tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa
    10321 caatccgatc atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag
    10381 gataaatctg gtatacgccg ctatacgatg aattatagcc catctttcca accagattct
    10441 gtacgtacgc ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt
    10501 ccaacaatca ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt
    10561 ctcattttat tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc
    10621 attcatacat tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg
    10681 gcagcagcag ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca
    10741 acgataactg tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt
    10801 gtcgaatgac aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa
    10861 gtagtttttg aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag
    10921 tattaacgta atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac
    10981 aatcaattat tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca
    11041 acgaaacaat acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa
    11101 atcccaatga aagagattac aagtaacgct tctttggcac taggagaagc ctatatggat
    11161 catcgaatcg agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca
    11221 aacagctttt tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg
    11281 aaacaaaaaa acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa
    11341 ctttggctag atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg
    11401 ttggaacaag cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa
    11461 ggcgaaacgt tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa
    11521 gagtataatg tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc
    11581 aaaagaataa aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc
    11641 gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt
    11701 tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca
    11761 gctttgatcc atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa
    11821 tatattttcc ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa
    11881 aacgatttgc aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa
    11941 cattggacaa agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc
    12001 ttctatcgaa tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat
    12061 atcgatgtga ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt
    12121 gcataattga ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat
    12181 gctccagaag tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt
    12241 ttctttatat tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt
    12301 tttcttcgtt atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa
    12361 aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa
    12421 taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt
    12481 atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga
    12541 agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga
    12601 taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt
    12661 ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa
    12721 tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa
    12781 gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat
    12841 cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg
    12901 tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa
    12961 tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac
    13021 aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg
    13081 atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat
    13141 gcatctggtg tccactgcat gactacccag gaattagatg aactggctca ttcagaagca
    13201 ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc
    13261 tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct
    13321 tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta
    13381 tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa
    13441 aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa
    13501 ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc
    13561 ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag
    13621 atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc
    13681 aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc
    13741 ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat
    13801 acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat
    13861 gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtacaga gcttcataaa
    13921 gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa
    13981 ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac
    14041 gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg
    14101 caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg
    14161 ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc
    14221 aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt
    14281 ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa
    14341 aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat
    14401 ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt
    14461 atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt
    14521 ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc
    14581 ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg
    14641 ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg
    14701 gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag
    14761 gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt
    14821 tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt
    14881 ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc
    14941 actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa
    15001 aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt
    15061 cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca
    15121 tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg
    15181 gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa
    15241 ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta
    15301 ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca
    15361 tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct
    15421 tgaagatcac accttataca cagttggcta atctgactgg acaaccagca atcagcttgc
    15481 ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa
    15541 gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac
    15601 caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc
    15661 tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga
    15721 agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac
    15781 actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt
    15841 ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga
    15901 tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat
    15961 cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag
    16021 tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa
    16081 aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa
    16141 attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg
    16201 aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga
    16261 tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt
    16321 gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga
    16381 agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc
    16441 gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc
    16501 ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc
    16561 ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt tgacagtttt aggtcttttg
    16621 gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc
    16681 gcactagtcc tgctgtttct gttatatttc gggttacctt atcaattaag tgccttaacg
    16741 gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga
    16801 tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc
    16861 tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc
    16921 agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca
    16981 gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat
    17041 attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat
    17101 ttagaaaaac gttctatcca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg
    17161 gcaccatttc tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg
    17221 taaaatgtaa atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg
    17281 aaatagaatc ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag
    17341 tcacgatgcc tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg
    17401 gaagccgaga atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag
    17461 ccaaactgtc atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag
    17521 aagtaaaaac tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg
    17581 aagaggagat gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc
    17641 ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa
    17701 gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa ttgtacaaag
    17761 aattgaaaag acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt
    17821 ttttagagga aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc
    17881 agcatgatta tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat
    17941 tggagtgaaa aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt
    18001 aaaagaagca ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt
    18061 acgtgattcc gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatccaat
    18121 cgctgtgatc caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag
    18181 ttggacagtc aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct
    18241 cagtgaagta ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa
    18301 agatacagag gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac
    18361 tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac
    18421 gagaaagcat gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct
    18481 ttttttccac gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta
    18541 tttcgataaa gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta
    18601 cggcaatata cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc
    18661 attgaagttt cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca
    18721 actcttgatt acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc
    18781 tggactggga gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct
    18841 gctagatcat ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt
    18901 gatccaatga ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata
    18961 ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg
    19021 atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa
    19081 cgattttgaa cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg
    19141 ctggaacagc tgcattagtc tcaggcgtgg atcccgttta cacaaaaata gatggtagtt
    19201 ggaaagaatc aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc
    19261 gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa
    19321 atatccaaga tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct
    19381 attctgctgg attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa
    19441 aagcatatca aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta
    19501 acactactga aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt
    19561 caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat
    19621 tttacgaaat agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt
    19681 cacgatctct cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt
    19741 tttattttat ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg
    19801 atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa
    19861 atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc
    19921 gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt
    19981 gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg
    20041 gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc
    20101 tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt
    20161 ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt
    20221 gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct
    20281 cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta
    20341 actttgcttt tttgtatttt ataccagcaa gccagtcaca atagcactta aaaagctgac
    20401 taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc
    20461 atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac
    20521 gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa
    20581 ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc
    20641 cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag
    20701 gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa
    20761 agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat
    20821 gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc
    20881 ttctttttct tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa
    20941 gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac
    21001 gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga
    21061 taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga
    21121 agctactcca ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc
    21181 aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga
    21241 aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac
    21301 accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt
    21361 tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac
    21421 aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct
    21481 gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa
    21541 ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat
    21601 ctttagtgaa attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat
    21661 aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac
    21721 gaaactatcg attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt
    21781 ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt
    21841 tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa
    21901 gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata
    21961 aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt
    22021 tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat
    22081 gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg
    22141 tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt
    22201 ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc
    22261 ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt
    22321 cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt
    22381 gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac
    22441 ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt
    22501 tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat
    22561 caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag
    22621 caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt
    22681 aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaagcaa gtagcgagct
    22741 ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact
    22801 cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga
    22861 tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg
    22921 tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac
    22981 gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga
    23041 tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca
    23101 atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat
    23161 gcttgtcgtc aactaacttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc
    23221 gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt
    23281 tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac
    23341 tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata
    23401 gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa
    23461 aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt
    23521 gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact
    23581 cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata
    23641 agataaaggt catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa
    23701 aaaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat
    23761 aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta
    23821 agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact
    23881 agaggagacg aacgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa
    23941 agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc
    24001 tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga
    24061 attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca
    24121 tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat
    24181 gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag
    24241 tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc
    24301 ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca
    24361 gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt
    24421 gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga
    24481 gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc
    24541 tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg
    24601 tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg
    24661 attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa
    24721 tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa
    24781 atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta
    24841 atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata catgaaaaag
    24901 aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg
    24961 aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg
    25021 aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt
    25081 ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga
    25141 tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata
    25201 ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca
    25261 attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc
    25321 aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca
    25381 atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct
    25441 ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa
    25501 cacttggttt taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc
    25561 ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga
    25621 aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata
    25681 aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat
    25741 ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat
    25801 taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc agataattct
    25861 gttccaaagg acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac
    25921 taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata
    25981 aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat
    26041 ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct
    26101 taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg
    26161 ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa
    26221 tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc
    26281 tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc
    26341 ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca
    26401 atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag
    26461 gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg
    26521 acaagttgca gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca
    26581 tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg
    26641 gtcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa
    26701 gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat
    26761 tggatgttta ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat
    26821 gatggacaat cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa
    26881 gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa
    26941 aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt
    27001 gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc
    27061 atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc
    27121 aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt
    27181 acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt
    27241 gaaaagttga cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg
    27301 ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa
    27361 ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata
    27421 ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga
    27481 acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc
    27541 ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc
    27601 ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag
    27661 atcagtcggc tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca
    27721 gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt
    27781 gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat
    27841 aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg
    27901 agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa
    27961 tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt
    28021 gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta
    28081 ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg
    28141 ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg
    28201 caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta
    28261 ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt
    28321 tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct
    28381 tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt
    28441 agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat
    28501 tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg
    28561 atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat
    28621 actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag
    28681 gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg
    28741 ctcttcaact tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt
    28801 agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac
    28861 tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt
    28921 tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa
    28981 tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc
    29041 gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt
    29101 attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat
    29161 ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc
    29221 ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct tctatgattt
    29281 tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa
    29341 taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt
    29401 aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct
    29461 tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac
    29521 agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa
    29581 cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc
    29641 ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa
    29701 cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat
    29761 tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc
    29821 tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg
    29881 cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt
    29941 cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga
    30001 acttaaagat tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt
    30061 ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca
    30121 aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga
    30181 tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat
    30241 cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa
    30301 gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt
    30361 gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt
    30421 ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt
    30481 tttctcttta ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc
    30541 gatccgatta actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg
    30601 attagttggc ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt
    30661 tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa
    30721 ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt
    30781 ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt
    30841 ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt
    30901 tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt
    30961 ttttgctgtc acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc
    31021 caattttagt ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct
    31081 ttgaaacgag gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct
    31141 gccggaacgt atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga
    31201 tgggaaaatt tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa
    31261 aaacagtaaa gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata
    31321 tgaggaattg aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat
    31381 ttctggcttt gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat
    31441 tccttatgac tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga
    31501 cttaaatgat gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga
    31561 taatacctat gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt
    31621 gacaaatggt gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc
    31681 tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc
    31741 tgaagaaatt attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat
    31801 acatgcaaat aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc
    31861 aaatgcagaa atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa
    31921 agccatcggc attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa
    31981 tat
//
LOCUS       DACEOM010000031        31922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEOM010000031 DACEOM010000000
VERSION     DACEOM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.032"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(151..2058)
                     /locus_tag="IW781_001363"
     CDS             complement(151..2058)
                     /locus_tag="IW781_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1182367.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="IW781_001364"
     CDS             2188..3063
                     /locus_tag="IW781_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1182368.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="IW781_001365"
     CDS             3143..4117
                     /locus_tag="IW781_001365"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1182369.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4306..5442
                     /locus_tag="IW781_001366"
     CDS             4306..5442
                     /locus_tag="IW781_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1182370.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5579..5923
                     /locus_tag="IW781_001367"
     CDS             5579..5923
                     /locus_tag="IW781_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1182371.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6194..7480
                     /gene="efmA"
                     /locus_tag="IW781_001368"
     CDS             6194..7480
                     /gene="efmA"
                     /locus_tag="IW781_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1182372.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7618..8217)
                     /locus_tag="IW781_001369"
     CDS             complement(7618..8217)
                     /locus_tag="IW781_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1182373.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8621..9742
                     /gene="mnmA"
                     /locus_tag="IW781_001370"
     CDS             8621..9742
                     /gene="mnmA"
                     /locus_tag="IW781_001370"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1182374.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9988..11409
                     /gene="arcD"
                     /locus_tag="IW781_001371"
     CDS             9988..11409
                     /gene="arcD"
                     /locus_tag="IW781_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1182375.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11423..12541
                     /locus_tag="IW781_001372"
     CDS             11423..12541
                     /locus_tag="IW781_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1182376.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12601..13893)
                     /locus_tag="IW781_001373"
     CDS             complement(12601..13893)
                     /locus_tag="IW781_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1182377.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14043..14603
                     /locus_tag="IW781_001374"
     CDS             14043..14603
                     /locus_tag="IW781_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1182378.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14732..15109
                     /locus_tag="IW781_001375"
     CDS             14732..15109
                     /locus_tag="IW781_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1182379.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15042..17114
                     /locus_tag="IW781_001376"
     CDS             15042..17114
                     /locus_tag="IW781_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1182380.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17325..18272)
                     /locus_tag="IW781_001377"
     CDS             complement(17325..18272)
                     /locus_tag="IW781_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1182381.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            18518..18982
                     /locus_tag="IW781_001378"
     CDS             18518..18982
                     /locus_tag="IW781_001378"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1182382.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(19044..20087)
                     /locus_tag="IW781_001379"
     CDS             complement(19044..20087)
                     /locus_tag="IW781_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1182383.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            20438..21301
                     /locus_tag="IW781_001380"
     CDS             20438..21301
                     /locus_tag="IW781_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1182384.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(21430..23052)
                     /locus_tag="IW781_001381"
     CDS             complement(21430..23052)
                     /locus_tag="IW781_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1182385.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            23301..23822
                     /locus_tag="IW781_001382"
     CDS             23301..23822
                     /locus_tag="IW781_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182386.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(24008..25738)
                     /gene="spxB"
                     /locus_tag="IW781_001383"
     CDS             complement(24008..25738)
                     /gene="spxB"
                     /locus_tag="IW781_001383"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1182387.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            25903..26721
                     /locus_tag="IW781_001384"
     CDS             25903..26721
                     /locus_tag="IW781_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1182388.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(26824..27021)
                     /locus_tag="IW781_001385"
     CDS             complement(26824..27021)
                     /locus_tag="IW781_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182389.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            27190..27768
                     /locus_tag="IW781_001386"
     CDS             27190..27768
                     /locus_tag="IW781_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1182390.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(28813..29316)
                     /locus_tag="IW781_001387"
     CDS             complement(28813..29316)
                     /locus_tag="IW781_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1182391.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            29675..30700
                     /locus_tag="IW781_001388"
     CDS             29675..30700
                     /locus_tag="IW781_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1182392.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            31023..31901
                     /locus_tag="IW781_001389"
     CDS             31023..31901
                     /locus_tag="IW781_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182393.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT        10117 a         5707 c         6514 g         9584 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttctcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagcagg gatctggggt
     5041 atggcaaaag cagttgaact gctgtcacct gaagaacgtg aagcacgtaa caacaaatat
     5101 aaaggattcc agcaattgat tcttgataca ttagaagcaa acgaaatccc ttatcaagta
     5161 aatggtgatt taacgaataa actccctcat gtgttgaatc ttcgcttaat tggcgaagca
     5221 aatgatttgc tactgatgaa gcttgatctt aatggggtcg ctatttctac tggttcagct
     5281 tgtacagcag gaaatatcga gccttcacac gtattagaag caatgtatgg agaagataca
     5341 ccgatactaa aagaatccat ccgtatcagc tttggtttgg gaaataccga agacgacatc
     5401 cagtatttcc ttgatcaatt agttcgtgct gtcaaaaaat aactagtgaa gtcattctta
     5461 tatgaacaga tgtctgagag aacgccttca ttttttaggg aacttaactg aaaaacctag
     5521 taaaaatcag gataaacttt tataataaag agaaactaat tttatcgagg tgaaaatcat
     5581 ggcatttgaa acaacagcaa cggtactggg cgcaccaatt agctatcgtt tgcatcctga
     5641 tgcaaaaaga tatacattgc gcgataatgg atttacagaa acaaacggag gcaattatca
     5701 attgatccga ccattagatg ccacgccgca aagcaaagaa ggattcaaat taaaaatcac
     5761 tgtatcaaaa gatattcaaa cattgaaaat gtcgatcact acagcaaatg gattgaaagc
     5821 agtcaatatt ttcaaagatc caaaacagaa aatgagccaa gataaatttt attttctgat
     5881 ggatgggatg attagccgtg ggttattcga aaaagtcgaa tgatctgtgg atataaaaag
     5941 acaactagtt ttgaaacggc ctattgttag atgactaact ttgtaaggac gacgcaaatt
     6001 agttgtcttt tttattttta aatttaacac atgttgttat ttttaatatt ttgctgattt
     6061 attgtttcta agtaaagaaa tttaaattga aacgattgtt atttagggaa aaaggtattt
     6121 tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg ctatacaaaa tgaaaaagaa
     6181 aagagggatg tttatggaaa atgaacagtc ggtcgtttta acgaattgga agagaaatta
     6241 tttgttcttt ttatcgggac agtttttgtc aggaatcact agtatggtcg tccaatatgc
     6301 gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca attttgagtt ttgctacttt
     6361 actaggaatg attcccatgg ttttactaag cccatttgta ggaccgctag tggatcgctg
     6421 ggataaaaaa gctcttttga ttgtcacgga tattattgta gcgattttcg cgcttatttt
     6481 agcagtagtc ggaacgattt cagaatcctt cccgatttgg ctagtttttg tgtctttgtt
     6541 tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc cagtcgatta tgccgacaat
     6601 cgttccatct tctcacataa caaggacgaa tggacagtta ggaatggtcc aatcagcgaa
     6661 tttcatcatt gcgccagctc taggtgcggc tttgttttca gtcgttcctg tcaattacct
     6721 tattttatta gatgtattgg gggcagtttt tggtgtcggc ttattgattt ttgtgaagat
     6781 cccaaaagtt tctcctgaaa tattggaagt tccgcttact atttttaaag atgcaaaatt
     6841 tgggctgcag caattgatgg ataataaagg gttatggtac ataacgatca atggtgcatt
     6901 tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca ttgatgacgc tagattactt
     6961 tggtggttct gttggacagg cgggagcagt ggaagtagtc tacgcagtcg gaatgctctt
     7021 aggcggtgca ctgatcagtt ttataggaac atggaaagac cgaatgaaac ctattattat
     7081 agcctacatc attatgggcc tgacgatcgg tgcaagcgga ttggttccaa acgatagtca
     7141 agggttcttg tactttctta tcttgaatgc aggagcaggt tgtgcaacgc catatttcaa
     7201 tacactgctg atggcaatga tccagcagag ttatgaatcg aatgttttag gtcgtgtctt
     7261 agggaacttc aactctttga tgaacctggc aggtccaatt ggattattat ttgcaggacc
     7321 attagctgat cgtttagggg tagaaaaaat gttcttgttt tccggaatcg ggattttatt
     7381 atgcggaatc gttttatttt taacgtcagc tgcccgaaat tatgataaag aattacagaa
     7441 aaaactggta aaagaacacc atgagcaaaa agacgaatag tatggaatgg agttgtgaca
     7501 gaagtcttgt tacaactcca tttctgatcc taatgttcct gtggcaataa gcctactcaa
     7561 atgaaaaata tgagtaatta tctttatttg tttaatactt gatatagggc agttttttca
     7621 cgacctatcg tacttcctct ttgcttcata gatttctttg atttgttgct cagataacgg
     7681 agtgccaaat ggtagttttt cctttaagag ttctctttcc ggtgcgtcta cgcctacatt
     7741 gacatcgttc tcgataggta cgctgtaatg atggatatgt ccatgaagat tgcggatagt
     7801 gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa tattgttgat ggtcgaattt
     7861 aacgatggca cctacatcat aaaaataaaa tttgggactt ccgtcagtaa gcctaggatc
     7921 atttttttct aaataacgga aaaaagcgcg actgtcatgg ttccctttga tcaagtggat
     7981 tgttccttgc agttgagtga ccagttccaa aatttctgca tttgcttttt catattgcgg
     8041 atgcatagca aggtcgccta gatgataaac atggtcggtt tcttttatta ctgcattcca
     8101 gctagtgatc aactgttggt tcatttcatc cacatttgca aagagccgcg gagcaaagtc
     8161 gttttttcct aataagcgtt catggaaaaa gtgcatatca ctgataaagt aattcatgta
     8221 tcttttgcct cccttttttc ttagagtaaa caaaatcact tatctgagca actcataaca
     8281 tctaaaaaga aaaggttgta aatgagacgt gcagctttaa gcgataagaa gcaattttga
     8341 aaaatagttt ttcacaattt ataaaattat tccttattgc cgaaaggctg gcccttttta
     8401 tgagtatgcc aattttttat atcgaatgct caaaagatga gtgatttttt ggttgactat
     8461 catgtttgtg tactgaatag gtccagtaga acagtatttt tacgacttga taattaaggg
     8521 atgaaatttt cagaaatatg acgtataatg taaaatactg aaaagttgcg accttcaact
     8581 cgtagatgtt tcagaatcta attgaaatga tggtgatatc atgacagaca acagcaaaac
     8641 acgcgttgtc gtagggatga gcggcggtgt cgactcatct gtaacagcac ttttgttaaa
     8701 ggaacaagga tacgacgtga tcggaatatt catgaaaaat tgggatgaca cggacgaaaa
     8761 cggtgtatgc acggccacgg aagattataa ggatgtagcg aaagtagctg ctcaaatcgg
     8821 gattccttat tactctgtga attttgaaaa agaatattgg gatcgtgtat ttgaatattt
     8881 cctagccgaa taccgcgcag gacgtacacc taacccagac gtgatgtgca acaaagaaat
     8941 caaattcaaa gcattcttgg attatgcaat ggatcttggc gccgattatg tagctactgg
     9001 acattatgcg caagtcactc gcgatgaaaa cggcgtggtc cacatgcttc gtggagtaga
     9061 caataataaa gatcaaactt acttccttag ccaattatca caagaacagc tttcaaaaac
     9121 gatgttccct ctaggcggca tggaaaaatc agaagtacgg gcaattgctg aacgcgcagg
     9181 ccttgcgact gctaagaaaa aagattcaac gggaatctgc tttattggtg aaaagaactt
     9241 caaacaattt ttgagcaact atctgccagc aaaaaaagga aatatggtca ctcttgatgg
     9301 tgaagtcaaa ggacaacatg ctggcttgat gtattatacg attggtcaac gtcaaggact
     9361 agggatcggt ggcggaggag attcgcaaga accctggttt gttgtaggaa aagatctagc
     9421 gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct gcattgtatg ccacaagctt
     9481 ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg ccaaaagaat tcaagtgtac
     9541 agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca gttcgcttat tggatgataa
     9601 tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc actcctggac aagcagttgt
     9661 attttatgat ggaatggaat gtctgggtgg tggtttgatc gaccacgcat atcaagaaac
     9721 aaaagtttta caatacgttt aagcgatatt aaatgaatca aaacagctat ggctaacaaa
     9781 agaaacacct tttcttttgg atagcatagc tgtttttttg caataaaatt aatttttatt
     9841 cattttatac ttgaaatact tgtgtaaaaa gttcataata tccaataata tgaataaagt
     9901 tgctttttgt taagcaagag cagttgcaaa atagaaggga atcttttgca agtgatttgt
     9961 tcaattttat aagtgagggt gatggaaatg gaaaagaaat cgacgggtat tagcaaatgg
    10021 ggactggttg ctctggttgt cagttcgtca atcggaagcg gtgtgtttgg gatcacaagt
    10081 gatctggcta gttctgcagc tccagggcca gcgatccttt catggataat cgttgggatt
    10141 ggtattttag ctttggtatt atcgctgaat catttaggag aaaagcgtcc agatctagat
    10201 ggtggtattt tcggttacgc ggaagcttca tttggtaaat taggtggctt catcagtggt
    10261 tggggttact ggttgtctgc gtggctggga aatgttgctt ttgcaacgat gctgatgagc
    10321 gccatgggag aatttttccc gatattcaaa ggtggacaaa atgttccttc tattttattt
    10381 gcaagtattt ttatttgggg tctgacttta ttagtaaaca atggagttga aggagcgagc
    10441 gtcatcaata caatcgtgac gatctgtaaa ttagtgccat tattcctatt tttaatattt
    10501 gtatttatcg catttaaagt gaatatattc actgctgatt tttggggtaa cgtatctgat
    10561 aatctagtga atggaacagg gcagcaagga acgatctggt tgcagattaa aggttgtttg
    10621 atggttatga tgtgggtctt tgtagggatc gaaggcgcaa gtgttttagc caaccgcgcg
    10681 aagaaacgtt cagaagccca gcaagcatcg atcattggtc tgctttgttt attgtttatc
    10741 tatatccttg cttcgatctt gccatacggc gtcttatctc aagaagagtt agcaactatt
    10801 tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg gaaattgggg agcagcgctg
    10861 atcaatatcg gcttgattat ttcgatcatt ggttcttggt tgtcttggac gatgctccca
    10921 gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc cgaaaggttg gggaaaaacg
    10981 aataaaaaaa atgccccaac ttattcgtta gttgttactg caatcttgac taatctattc
    11041 ttattgactt tcttagttac tgattatgcc tatcaatttg cctattcttt atgtacagcg
    11101 gcaattttga tttgttatct acttgtcggg atctatcaag tccagttttc tttaaaagaa
    11161 ggaatcaaaa aacaagtagt gatcggtggg attgctgtag tttttgaatt gatgggcatc
    11221 attttagcag gattttctta tgtcctttta tgtagtattg cctatcttcc tggtttttgc
    11281 ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta cgggaaaaga aaaagtaatg
    11341 attgggctga tttgtgtggc cgcactgctt tcaatcgggt tgcttgctgt aggctggatc
    11401 aagatataag ggggaaagaa acatgaaaat cgcaaatttc ggtgtagaag aatggctaaa
    11461 taaatgggaa aagaaagcgg tatatgatat tgcacaaagc tcgatcgaag cattgacatt
    11521 agacgaactc gttggcttag atgggactag cgtaagtaaa tttttcgaaa aacgcaaaaa
    11581 cgaaccattg gactatggct ggatcgaagg atcagatgtt ttcaaacaag aagtcgcttc
    11641 tctttatcaa accgtcgatc cggaaaacat cttacaaacg aatggagcga ctggtgctaa
    11701 tcttttagct ctatacgcgt tagtggaagc aggagaccat gttgtttcga tgcttcctac
    11761 ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc gctgttgact ttgttcattt
    11821 gaaggaagaa gaagattggc agtttgattt agaacagcta gaagccttag tcaaacctga
    11881 aacaaaaatc atctgtttga atagtgcaaa caatcctaca ggcacattgc tagatcggaa
    11941 aactttagaa aaaatcgcag aaattgctcg tacagtagat gcatatgtat tgatagatga
    12001 agtatatgcg cctttaacgg ataaaggtga attcttgtcg atcgtagatg tctatgaaaa
    12061 aggtatagca accaattccc tatccaaaac ctactccatt ccaggtattc gtattggctg
    12121 gacagctact ggacctgagt tggcagaagt ttttcgaaag tatcgagact acacgatgat
    12181 ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca ttgaagaata aagaaaaaat
    12241 attggcgcga aacaaaaaga tcattacaga gaaccttgtg attttaaaac aatgggtagc
    12301 caatgaaccg aaagttgaac ttgtcgctcc caattacgtc tctacttctt tcattaaatt
    12361 agccatcgaa gaagatgacc agacattttg tatcaattta ttagaagaaa caggagtact
    12421 attagtacca ggaagtgcct ttgatttacc gaagcatgcc cgactaggtt actgttgtaa
    12481 aaaagaaacc ttggaaaagg gattattcct cttatctgag ttcttgaaaa aacaggccta
    12541 aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa gcagtgtttt tcatttacgc
    12601 ttattgtcta tgagaaggag agtgccctga agctatggag actcctgaag ctttttcttc
    12661 tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt acaagcaaga agttgaccaa
    12721 aacaagaaga gaagtcgaaa gaaagacgcc gcgataacca aatgcactgg aaacactgga
    12781 gccgatcata ggccctacta cgttaccagt tgcttggaaa gattggttat aactgaagat
    12841 ccgtccagca gcttgatgag gcgaatattt tgtgatcaat gcttggacag cagggagcag
    12901 acaagcatca gagataccaa tcaaaaaacg taaggcagct aattgccaaa cattcgtgac
    12961 gaatgccatt gggatataaa ccagaatcgc aaaacccaaa ccaatcgcaa gtattctttc
    13021 actcccaatc ttgtcaccta agcgaccaaa tcttggagcg gctatcaaag tggcaatccc
    13081 aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc ccatgtccat gcagcaattg
    13141 gcgaatgtac agactgatga ttggactaat ggaattgttg gatgcttgga tgatcatcgt
    13201 tgtgacgaac ataccgatga cgacgtgtgg atatttcaag tctttgaaaa tctgtttggc
    13261 agaaaccatg ttttcttttt tcactgggac aaatttttca tggacgaaaa acagactcaa
    13321 caagaagacg aaaaataaga tgatccctgt aatgaaaaag gtagggcgat agccaaacgc
    13381 tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt cctgtaacag aaccagttgc
    13441 taaggttcca agaacttgac cacttttttc ttttggcgtc ccagttgcga caagggcagt
    13501 ggcattactg atataaccag aaaaaatccc ttgaagcaga cgcaatgcga ctaattgata
    13561 cacactagtc acgcaaccca tcaaagaaat gacgattgcc attcctaatg aagcacgcaa
    13621 cagcatcaat tttcttcctt tttggtcggc taattttccc caccaaggag agataagcgt
    13681 agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa aagttcaatt gtgacgtact
    13741 ataattacct aacgtatcta tgtaaagtga cataaaaggc atgactaaac taaatccgat
    13801 accagccata aatgttccaa accataagac aaacaagttt tgttcccaag tttctctttt
    13861 cctaaataat ttttccttta ttcttgcagc caattagact ccttctttct gaaaattttc
    13921 atctacagct ctttattata aactacaata tgtagtttat caatgaaagt ctcttttttc
    13981 tctttctttt tcgtggtaaa ctgaaagagt gaaggattta ttttatgaga ggaaacatgg
    14041 aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga ctgctttcga gaactagcag
    14101 aagagatgga tgttcagcaa ataacctttc agcatttagc caaacgcttg gatatcaagg
    14161 ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt caaaacagcg ctaacagcga
    14221 aacttctgca ggaacttaat gaccagctgc gacgggctct tgtgggaaaa agtggtcagg
    14281 aagcactgca gatctatgca gaaacttatc gatcctttgc tttttcaaat caagcagtgt
    14341 atgagttact gatcagtgta ccgcatacca atgaagctgt tttattagat ggaatccatg
    14401 aaacgaatca gatcattttg cagatatttg aggcttttca attaacgaaa gaagaaaaag
    14461 ttcatcgaag cagggaactt cgtagtatga tccacggtta tctttcctta cgttttttag
    14521 gatactttac aaaagaacca acaatcgatc cagaagacag ctatttttgg atgatcaata
    14581 actttatcgc tactttgcct taaacaatca gattcatttg taacaatttt aagatgttca
    14641 ttaaatgacc cttttataag agaaaattct tgcaactttc ttgaaaagtg actaagatga
    14701 gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa gtaattacca tgttcgggga
    14761 caatgagcct tggtggtttt ttgaagactg ggaagaagat attgaagaag aagaaacatt
    14821 cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa tggaacgaaa tccatcaaaa
    14881 ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc ttttggaatg acggagatga
    14941 gcgctggtgt gaagagtgtg acgatgattt acagcaatac aagggacttg cgttgttgaa
    15001 aaatcatcag cccatcacag tagaaagcag aaaggaattt tatgaaacaa ctaattctag
    15061 cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct tggtgctaat caaaagcaca
    15121 aaaattatta tgaaggaccc aaggtgatcg ttacttgggc ccttggtcat ttattaggat
    15181 tgaaaatgcc ggaagatctg aataaggaat ggcagagctg gcaaatggac acgttgccaa
    15241 tgatccctaa aaaactagga atcaaaccac ttcctaaaac aggacatcaa ttaaaagcaa
    15301 tcaagcagct ggctcagcga aaagatgtct cagaagcagt cattgcgaca gatgccggaa
    15361 gagaagggga acttgttgct cgctggatct tagagtatgt gcatttcaat aaaccagtga
    15421 agcggttatg gatctcttct caaacagata aagcaatcaa aactggattc aagcaactaa
    15481 gacctgcgaa agaatatgat gcattgtatg aatctgctct tgcccgggca aaagcggact
    15541 ggctggtagg attgaatgtg accagagcct taactgtgaa gtacgcggat aatttatcag
    15601 caggccgtgt gcagacaccg acactagcat tagtgagaga ccaagaaaag aaaatcgagc
    15661 agttccgtcc acaaacgtat ttcacgatca cgttgcaagc tggaaaagaa caagccaaaa
    15721 tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga agcacaaaaa cttgttcaat
    15781 tgatcagtac aagcaaagga actgtcacag aagtccaaga aaaattaaaa acggaatctg
    15841 cgccattgcc ttatgattta acagagatcc aaagagaagc caatcaacgc tatggctatt
    15901 ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga gacccataag atcgttactt
    15961 atccgcgtac agacagcaaa tatctgacaa atgacatgaa aagtacgatg aaagaacgat
    16021 tgcaagcagt cagcgacttt gctcctgagg tcaagcagta tttgaaaaac ggagccatcg
    16081 tccaacagac aaaagtattc caagacagta aagtgaccga tcaccatgca cttttgccaa
    16141 ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga gcgaaaaatc tatcaaatga
    16201 ttgtctctcg ttttttagga ctgtttgctc aaccccataa agtcagccaa acaaaagtta
    16261 cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa tcgtgtcgtc caagcaggat
    16321 ggaaaggcga aactgaaact gagacggaaa cagtcaaatg ggaaaaagga atgagaatca
    16381 atcccgattt cgccatcaaa aaagaactat ctgcaccgcc taaaccattg tcagaagcaa
    16441 gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc agctacgcgt gcagagatta
    16501 tcgagaaact aatcaaatct gaactgatgg aacgtacccc tcaaggactg caagtatctc
    16561 ctaaaggtaa gcaattatta gtattggtaa atccctcttt agtaacacca gatttgacag
    16621 aaagctggga aaaagacctt gaagctattg ctgcaggaaa gaaacaagca ggaaccttct
    16681 tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga gatcaaatca agcaaacaag
    16741 aatatcaaga tttctcgatt acccaaaaga aatgcccaga atgtggggcc aatctgcgcg
    16801 agaaaaacac acgtgacggc aaaatctatg tctgcaccaa ttcagattgc aactaccgca
    16861 gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca atgtcaccgg aaaatggaaa
    16921 tcatcgaagg taaaaacggt gcttatttcc gttgtaaata cgatggaacg acagaaaaat
    16981 tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca tgaagaacga cgcttgctga
    17041 aaaaatatgc acaagcagaa gaaccagaag aaagcccatt agcagcagca ctaaaagcag
    17101 caatgaagaa gtaataagat atgaaagagg ttgtgataga agtggtcagc ttcaagaaat
    17161 aagaaggaat ttacgaaaat tgcttctcaa atttttgtga aatttcggct tatttccgaa
    17221 gaagctactt ctggaacacc atttattcgg aaaaaaggac tgtgacaaga cttttgtcac
    17281 agtccttttt catttaaaaa aatgtttact tcatttgacg tatattattg gtgaggcttc
    17341 aattcatttg gcttcacttt ttctgtttct cttatgatat aaacaggacg ctgtttcgtt
    17401 tctaagaaga ttttcccaat atatttcccg atgattccaa gacataacaa ttggattcca
    17461 ccaatgaaca ggaagatcga gaccatggaa ggccatcctg atgtaggatc accaaacaac
    17521 aatgcccgca aaataatgaa caaaattgca ataccagaac cgacacaaga caaagcgccg
    17581 ataaatgaag caatcgttaa cggtgcatcc gagaagttca cgataccatc aatcgagtag
    17641 ttgaataatt tccagaagga ccaagatgtt tcgccagcca cacgttcgcg gttttcataa
    17701 gaaatatatt ctgttttaaa tccgacccag ctaaataatc cttttgaaaa tcggttgtat
    17761 tctgttaatt ctaggacggc atccaccatt tgacgagtca tcaagcggaa atcacgagcg
    17821 ccatcgacca tttctgtatc gctgatttta ttgatcaact ggtagaattt tttcgcaaag
    17881 aaactgcgta tcgcaggctc gccttcacga gtcgaacgcc gggtgccgac acaatcgata
    17941 tcagaagtag tgatccggtt gatcatctca ggaagcagtt cgggcggatc ttgcagatca
    18001 acatccatca cggtcactaa atctcctgta gaagcttgca atccagcgta cagagccgct
    18061 tctttcccaa aatttctgga aaatgagaca aagcggacat aaggataagc tgaggctagc
    18121 ttcttgattg ctgctaacgt accgtctttt gatccgtcgt caataaacaa atattcgaac
    18181 tcatgggcac ctttgaattt ttcaacctca tcaaaaaaaa gaggaacagt tttttcttca
    18241 ttaaaacatg ggacaacaat tgatactttc atcgtttcta tcctttcaat cgtatgttta
    18301 tatatggttt acattcaaaa tttaataaac cttaaataac acctttaaaa tttgatttta
    18361 cgtaaaagaa caggtattct ttttgtaaac taacgtattt agtttaccat gtttcctcgg
    18421 tttatcaaac ttttaagaac aatttttatt agttggcaag tagttttgga tagagaaaaa
    18481 ggaggaagtt ccagtctatt gactaggagc aaacaaaatg aaaatacaca cacctatcgg
    18541 ctttttatgg ttagatgcag atgaatccgg attgactgct gtatcttttc agccaatttc
    18601 agaaagcgaa tcagcgaatc agcgaatttt aacgcgcaca caagaacagt ttgaagaata
    18661 ttttgcggga aaaagaaaag aatttgatgt tcctttatct ataaagagag gtacgccttt
    18721 tcagcaaaat gtctggaaag ctttgtcgac cattccttat ggagaaatcc gaacgtataa
    18781 agaagtagcc gttgctgttg atagtccgaa agctgtgcga gcgattgggc aggcaaatcg
    18841 aaccaatcca ttgccaatcg ttattccttg tcatcgagtg atcgggcaaa atggaaaact
    18901 gacaggatat atgggaaacg caatagatgg ggttgaaatc aaacgccaat tgttgttgtt
    18961 agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca tgcgagaatc
    19021 acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag taaagccatc
    19081 actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc ttctttcatg
    19141 aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc aaaccagctg
    19201 ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct gttgaccaaa
    19261 caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt tttctgcgta
    19321 tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc aaacagatag
    19381 acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa ttcgacaaca
    19441 gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag tgtaccaccc
    19501 aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag aaaagggaga
    19561 gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc tttttcttcc
    19621 tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca aacagcgttt
    19681 cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc aatcaagtaa
    19741 gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt tacaccaatt
    19801 tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa ttctaatagt
    19861 ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa agtatttcct
    19921 aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag ataaatagca
    19981 agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa gatttcgcgg
    20041 accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg atcggttcct
    20101 ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag tattttgaca
    20161 aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat aaacggtgtt
    20221 ccagaagtag cttctacgga aataagccga aatttcacaa aaatttgaga agcaattttc
    20281 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctctt attttgatta
    20341 gaacttgcga aaataaaccg gttaaagtgt gtctagcgca agagatttgc tataatagac
    20401 gataagggta aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg taacagattc
    20461 ttgttgtgat ctgccatacc atgtattagc aaaagaaaaa gttgattttt tatcaatgaa
    20521 tctccaattg aatgatcaag agttagtaga tgacctcgga aagacattca attatgaccg
    20581 ctacatgaat cagctgaaaa atggtgcgat gcctacgaca tcacaaatca atgttggccg
    20641 atatttagaa tttttccgtt cttatgcgga aaaacagatt cctgttttat atgtggcttt
    20701 ttccagtgga ttaagcggct cttacagtag tgcgcttcaa gcggttgaat tgctgaagga
    20761 agaatatgaa gaccctgaga tctatgtttt tgatacaaaa gctgcaagta ttggggaagg
    20821 gcttttagta ttggaagctg taagattgaa aaaagaaggt aaaagcatcg aagaaaccct
    20881 agagtggctg gaaagtaatt atttgaaagt ccattcatgg gtaacagtag acgatctgaa
    20941 atatctagaa cgaggcggta ggatttccaa agcaagtgct actcttggca gtctattgag
    21001 tgtaaagcct attattacgg tggataaaga aggaaggctg acgaatgttg acaaagttcg
    21061 aggaagaaac aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa tcgttgcacc
    21121 acttgatcaa atcgtttatg tggcttacgc tggggatcta gagagcgcgg agaaggcaaa
    21181 acggcttttg gaagagcaga taaagatgaa agatgtgaag ctttatccct taggaccaac
    21241 gatcgccagt cacacaggct atggctgtat cgcgattttt tcgatgggaa tcagtcgata
    21301 aaagcagtaa ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa acagcgactc
    21361 ttaaaagata gatgagcatc ctatctctta gaggtcgctg tttttttatt ttttaagcta
    21421 aataggccat tatgcccata actcgttcaa ttgtttttga atatctgcat tttccaaaaa
    21481 ttcatcatat gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg caatgatgcg
    21541 gtttgccaat gtttggataa attgatggtc atgtgacgca aataagattg agccagtaaa
    21601 tgccatcaag ccgtcattca atgctgtgat tgattctaag tctaaatggt tcgttggatc
    21661 atctaataca agtacgttag ctttcgaaag catcattttt gaaagcatgc aacggacttt
    21721 ctctccacca gataaaacat tcactggttt caatacttct tcaccagaaa acaacattct
    21781 tcctaagaag cttcttaaga aggtgttatc atcttcttct ttacttgcat attgacgcaa
    21841 ccagtctaaa atcgttagat cagtatcgaa ttctttcgtt gtatcctttg gtaaataaga
    21901 attacttgtt gtcacacccc aacgtactgt tccagtatct ggcgtaattt cacccatgat
    21961 aactttgaat agcgttgttg ttgttatatc attttctgcg acgaaagcga ccttgtcttc
    22021 tttgctcaaa ttaaaagata tatcatctaa aattttcttt ccatcaatcg ttacacttac
    22081 attttctacc tgcaataagt cgtttccaat ctcacgttca ggtttgaatc caacgaatgg
    22141 ataacgtcta gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca tttttttacg
    22201 tgaagttgct tgttttgatt ttgatgcatt cgcactaaag cgggcaataa attcttggag
    22261 ttctttgatt tgttcttctt ttttggcatt tgcgtttgct tgaagtttag ccgctagttg
    22321 actagattcc aaccaaaaat cataatttcc tacataaagt ttgattttac caaaatctag
    22381 atccgccata tgtgtacata ctttgttcaa aaagtgacgg tcatgggata caacgatcac
    22441 agtattttca aaattgatca aaaattcttc cagccaattg atcgattgga tatccagacc
    22501 gtttgttggt tcgtctagca aaagaacatc aggtttacca aatagtgttt gtgctaataa
    22561 cacttttacc ttttgtccac cagttaattc actcatttgc tgatggtgca attcttcagg
    22621 gatgttcaga ccttgcagta aaacagctgc ttctggttca gcttcccatc cattaagctc
    22681 agcaaattct ccttctaatt cagctgctcg aataccatct tcgtcagtga aatcctcttt
    22741 catgtaaatc gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc ccatgatgac
    22801 tgtttcaagt acagtatagt cttcatagtc gtaatggttc tgtttcaata cggccatccg
    22861 ttcatctggt cccatagtaa cggaacctgt ggaaggctgc agatcccctg ataacacttt
    22921 aaggaacgtt gatttacctg caccatttgc accaatcaag ccatagcagt ttccgggggt
    22981 aaatttaata ttcacatcat caaatagttt gcggtctgaa aagtgcaaac tcacatcatt
    23041 tacagtaatc aatttttttc ctcactttta tcattattag ttttgacaag tcttatagaa
    23101 ttatataggg gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt ttcacgtgaa
    23161 acagaatgaa aaaaataaaa ataaaagcgg agcgaaaata gctactatag gatttattag
    23221 tttgcaacag cataattttt gacgttattt ataagaggga gtactataaa actgtaaaag
    23281 aaatgaaaga aggattgaaa atggatgaaa aaagaaccat cgactggggc tcattgattt
    23341 tgggaatttt atttgtactt gtctcattgt tatcatttca agaccctgtc ggaaatttag
    23401 ttgcaattgt agtagtcttt gcgatttttg cctttattaa agggatattt gaactgtttg
    23461 tacggaacag attaaaagag ctaacaggat ataaaggaaa gatgccctta ataatcggga
    23521 tcatcgatat tctagttggc gtctttttct tattcaatat tggtgcagga gtcgcagcat
    23581 tgccttttgt ttttgcagta tggtttattg ctgattcagt tttagcgtta tttactgctg
    23641 acttggcaag aggagtaagt gaaggatatt attggtttac gatcatcgtc aatattttag
    23701 gaattttatt aggaattttc ttattgttta atccaatttc ttctgcgttg acattaagct
    23761 ttttagttgg attctatttt atgttatttg gtatcacaca tatcgtctat gcatttagat
    23821 aagtaaaatc aaacaaataa aaaagccggc ttccgattac agttttactg ttgtaattgg
    23881 aagccggctt ttttatcatg gacaaatatg cacgaggtga tttttaggtg tcgagacaaa
    23941 tttatgcaaa aatgtgagaa gctatataca caaattttat cttaatactc gaagcatagc
    24001 gcagttgtca caacctcttt tcatataatt cattcaatac tggcaaatca tggacttcat
    24061 atttttcttt gaatgcttgg atttcttctt cagaatattt tcttggatct aaatgcagat
    24121 cttctactgg caatgggcgc ttgttattga tcttcacatc gattactaca ggacgatctg
    24181 attttgcagc tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact gtaaagctat
    24241 ttgccccgag cgcttctcca actttaccga aatctgcatc ttctaaaaat acaccaaatt
    24301 tcttttgttc cgtatcttct tgttctgctt cgatgaaacc aaaagaatca ttcgaaaaga
    24361 caacattaat aattggaagc tgatatttga cttgtgtaat gatatcttgc atgaccatag
    24421 caaacccgcc atcaccactt aaggtaaata cttgtttttc tgggtagctt agttgtgcgg
    24481 caattcctcc tggcacacca ttacccatag ttgcgaacca tccagatgtc gtatgttttt
    24541 gtttaccgtt catattcaat aaacgaatag aatggatagt cgtgttaccg acatctgtga
    24601 cgaaaatcgc atcgtcttca gcaatacgat tgatttcttt atagacagcc tctggacgaa
    24661 tcggttcttc ctctcgatct tcaaaagatt tcaaccaatt tacccatttt tctttatttt
    24721 cttgatttgc taggtaccag ctatcagcag ggcgagcatc ccctaactcg acaagtcttt
    24781 gcaaagctgt gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt cgtccaaatt
    24841 ttgacgcatc gatatcgatt tggataaatt cagcagttgg attaaaaaat gcacgtccaa
    24901 atgggaagtc gctaccgaca aataagatca gatcggcttc tgccaatgct tcatttccgg
    24961 gtttagtagc gacacgtcca gcaaaaccaa gaaagttttc ataatcatca ggaataattc
    25021 cttttgctag aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat tctttgattt
    25081 gcggaaatcc gtttcgcgtt ccttgaccaa cataaagaac tggttttttt gctttttcaa
    25141 gataaggcaa agcagctagc aaatcttttt cttctggtaa tatcacactt gttttgtgag
    25201 tgtgagcggt ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca acagggatag
    25261 tcacgatagc cacgccttta tgctcatagg cggctttaat ggcttcatct actacatgcg
    25321 gaagactttg tggtgtcatg actgtacgat tgtatacgct aacatctgcg aaaataggat
    25381 tttcatttag ttcctggaaa gagttatagt tcatggatgt agaagcaact tgtcctaaga
    25441 acgctactag aggcacatgg tccatctgtg catcatacaa accattgatc aaatgtgtcg
    25501 caccaggtcc tgcagagccg aagacagcgc cgactttccc cgttaatttt gcatctgcag
    25561 cggctgcgag tgcgccgact tcttcatgac gaacttgaat gtagttgatt tcggtttttt
    25621 cttgatatag cgcatccata attgaattga aagatcctcc gggaatgccg taaatatggt
    25681 cgatacccca gctttcaaga acttttacca ttgcgattcc tgcattgatt tttgacatgg
    25741 tgtttcactc ctttagcaat gatgatacat aaattttttg tatctagagt atagaacttt
    25801 ttatcgaaaa tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt tttgggaata
    25861 tgctagagta gtacatagat agattcaaga gaggggaatg gcatggttga atggctattg
    25921 agacttgaac cttggcagca agcacttgct gggacagctt ttacttattt tatgacagct
    25981 cttggtgccg ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct aaacttgatg
    26041 ttaggatttg cttctggtgt catgattgct gcaagttttt ggtctttgtt agatccggct
    26101 attacaaaag cagaagaaaa tggagatatt gcttggctag ttgtaagtat tgggtttggt
    26161 ttaggcggtg tgtttttata tatggcagat aaaacactgc cccatatgca ttttggacca
    26221 caacatgaag cagaaggatt gcctacccat ttaaaacgga cgatcttgct tgttttttct
    26281 attacattgc ataacattcc tgaaggttta gccgtggggg tagcattcgg tgcagcagca
    26341 actgcggata atccgacagc cgctatatta gccgctgttt cggtggcgct aggtattggt
    26401 atccaaaact tcccagaagg agcagctgta tcaatcccgt tgcgacaaga aggtctgagt
    26461 cgtaaaaagg cctttgttta cggacaagct tctggaatcg ttgaaccaat tgctggagtg
    26521 attggagcgc tactagtaac gaaagtggag ctgctgcttc cttatgcact tgcttttgcc
    26581 gcaggagcaa tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca gcagacattg
    26641 tccagcaaac gtcattttgc agtttttggc gtcatgagtg gatttatcat tatgatgatt
    26701 ttggatgtgg cattaggata aataaaaaaa atatatatga tatatcaaat agggccgcga
    26761 acttatatcg cgaccctatt tttttagtaa gttatatcat aatgaagtga atggtatcat
    26821 acattaataa tagttttttc tatgtgtatt tcctttgatc cagcgaacaa gccagatgat
    26881 aagtgcgatt gggattagaa ctttgaccgc aaaccataaa agcccgctta ggacactgac
    26941 aaaaagactg atgacgaagc tagctgcaat tagcactaaa agaaccatga agatatttaa
    27001 gaacatattt ttttgacgca tacaaaaccc tcctcttgtt cattctttac tatagaaaaa
    27061 gtataacagg gattgtttaa aaacacctcc aactgaagac tgatttttac ttcatctact
    27121 taaatgatca aacaattgct ttttctctgg tttatcatct aaaatagagc ggaaaaggag
    27181 gagaatatca tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat cgcgattatc
    27241 atgactattt tagttttaga acttcatgaa cctaacggag acacgtggga ggcgctcgct
    27301 gcattaggtt accgattttt tatctatatt gtcagtttta tcattttagc gatttattgg
    27361 aacaatcatc atcatttatt tcaagttgta cataagataa atggacgggt cttatgggcg
    27421 aacagtttct ttgtttttgc gctttcgctt tttccgtttg caacttcatg gatcagtgag
    27481 tacatcaata gcctggctcc agaaatgact tatggttttg ttaccttgac cgctaatatt
    27541 gcgtattata tcttggcaaa agaacttgtc aaagcaaata aaggccacga gaaagcattg
    27601 aaggcgatgt tgaataatta caagaaatcg tatctatcga ttggtttgaa tgtgttagga
    27661 attgtcttag ggtatttagt agcgccagta gccgtgctga tcgcaaacat cgtgattcta
    27721 gctgcttgga tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa tgatccgtgt
    27781 ttaaaattta agagaaaaat atagaaaaaa taaataaacg atcaaatatc cgagtgatac
    27841 tagtattcaa agagggatgc agatggatca aaaaagaatt catgtaggac tgattactag
    27901 ttttgtcacg atgcttattt atttggatca aggtcatttg acttggacag tagttggtgt
    27961 cgtaggattt ttgacattat tcacagcaat aaaaaaataa acatctttat gtaatctgat
    28021 gttctatcta tttaagaaaa gcatgtttac agcagtatga ttctgttgta aacgtgtttt
    28081 tatttgtgga aaatttaatt tttagaaaag tgtatcgaaa aatagatagt tagtttttcc
    28141 acagtggata acttcgtaag agataaaaat aaaaaattag tcaaagaagg gcgtatagat
    28201 ttcttttgat aggagatata caacagaaat gaataaatat taacgtgttt tcaaacagta
    28261 taaaacggtg tggtttttgg aataaaaaaa taacatatct cattttacac ttttgttatt
    28321 ttatacttaa aaaataacga cacagaaaat accgtgtctc agatttctct actacttcta
    28381 aaaactactg tttttttcct ttgaaatgcc ttcaaaaagt aggatcaggc agaatatact
    28441 ggcagaatca aggagaaggg agcctaaata aggagaaaaa ccatagaaaa tataaagtaa
    28501 ttacttataa aacaagcttt ttatagaaaa ataatatacc tgagtagggt ataaattaag
    28561 acggaattcc tttctagatt gagatattga ctaggaagaa tgttaagatt ttaagccttt
    28621 aagctaaaaa aaaacccttt ttgttaaata aggggaatat agcctgtcag tttcaaactg
    28681 caagtcgtcc tgctgcaata atatagtaga ttagagatga aaatagattt ttttaaacca
    28741 atgactgaat aaaaagaccg tcatagatac acattggaat gtgtctatta cggtcttttt
    28801 ttacgaacga ttttattgaa gacggacagt tcgggtcaaa aataaaaaag ctacgatcag
    28861 aaataagaat aaaatgtatc ccaaaataat ggctagacgg tctaacttat ttgtcgatag
    28921 atgtttgttt agaagttgat aggcccataa acctatcagt cctcctgttg tattagttat
    28981 caaatcagtg atgtccgcta cgccaatacc gaataggaat tgaatcaatt ctgccaagaa
    29041 gcttacaagg aaaataatcc ctaatttttt agaaaaagaa agtctcttga aattgacatt
    29101 caaaagcacc ccaaaaggaa aaaagacaat caagttataa gtggtctcac gtaataccgt
    29161 cgtattttca aaagaaaaag tagaaaaagg gacgagattg acattgctgt aagaataagt
    29221 gagaaattgt ggaagattaa atgatagctt gaataaaatc aaccataata gaaagaataa
    29281 ataaatccct aataaaaatt ttgatacata ccgcatagac atctcctcca tttaaaaagt
    29341 ataccatcga aaaccgctga aaaatcataa aaaagtatga agtttaagga ccaacgtagt
    29401 tatcgtacga aaattagaag cttttttttg ttgggaatcc ggtttcttta acaaagctaa
    29461 gatagcgagt tgcttggaaa agttgctatc ttttttctgt caaaacgact cggaatgttc
    29521 gttttttgtt ctaatttctc caagttgttt actttttgat actaagttta tgaaaaatca
    29581 agaaatagtt gttatactag gtaatgctga caaacgggat tggcatttca tcttggttca
    29641 gctttttttc caattactag gaaggaggga tagattgttc aagacttatt ttcgtttcaa
    29701 taaaatacaa gataatcctt ttcgtatcat gaatctgatg ctacttatct ttattgtatt
    29761 atggttaggt tacataaatc atgatttttt gatttcaagt ttttcatcat tagggatctt
    29821 tacttttttc tattatcaaa acataccgat gaaatatttg ctgaaacgta tgtttctgat
    29881 cggttgcgga ctgttggttg ctttcgtttt ggggatctta tcgacttatg ttttatggct
    29941 tgaaccattt gccgtagctt tggttgcttt ttccagcaga ttcgtgttgc gattattcca
    30001 tatttctaaa ccaggtggct tgttttttgc gatgctttct gccatgggaa cgagcatgca
    30061 gctgccaatc gctcaattac cgatcgtctc actttatttt tttatgggtg tcgtttttgc
    30121 cctgattgcg gcagtgatta caaaattact ggattcacgg cctgagcaga caattgaaaa
    30181 agcaacttta aaagaaagat ttcacgaaga gcctttagtg atcattgatt ctgttttcta
    30241 cagcgctgcc ttatttcttt ccgtttatgt cagtcacggc ttgaatctgc ataatcctta
    30301 ttggttgacc ttgtcatgtg catcgatttt gctagcagaa aacttggatg cgatgaagca
    30361 tcgacaagtc caatatctga ttggatcgat gggtgggtta tgtgtgtcgg cattcttgtc
    30421 atttgtgccg tttacgcagt tgcagaccat ttttctgatc acttttctat atggaatcgc
    30481 acaatttcta gttgctagaa attatgcagt tgctaatatt tttcttaatc cgatggcgtt
    30541 gatgctatct acacttattc gtggagcata cttgatttct ttgatcgaat atcgtttttt
    30601 agggatcgtc ataggaagtt tcatcggttt aggtgttgcg tgggtaatga cggttggttt
    30661 gcaacattat ttaacggttg ttcgtcaaaa attagaatag aaggacaaat atatgtgcca
    30721 aaagaggtta tgacaagatt tttgtcacaa cctctaattc aaggtgttca aaagccagat
    30781 tctcagcatt aagtcaaatt cacacaaaaa tgtggaaagg aactttcaaa aaaattttat
    30841 atttattcta tcaaaaaatt ttgtttatga tggtaactga ataaactttc ctgagatggt
    30901 caatgctttc cttgaaagtc ttacagatat tctttcttca tagaaagttt aagttttata
    30961 gttagtgtac aataaaagta tgtttatacc atatggtgtc cggataatca gtcatttaaa
    31021 caatgaggtt caaagatatg aaaaggggaa atttgatgaa gaaaataagc aatgaaatcc
    31081 aaattctggc agaaacttta actcttccca aagtcgttat agagcaaatg attgtacaag
    31141 caaaaaacat ccttgatgag gtgtttgagc aatatagtca tgaactaatg gatcctaata
    31201 aagcaaaggc gactgcaaag aaaatagaca aagtctatga agaggaacct tttgcagcat
    31261 tagctattta tctatatgca gcttctcaaa gttggaaaaa aatttatcaa ccattgaaga
    31321 ttccaagaac tatctatttg gcaacaatga atgcttttac tagatttatt catgaacatt
    31381 tccaagtaac aggaagctat cgctttgacc gtggtttttg gatttggcat tacactagtg
    31441 gcttgatctt tcggattggt gaattggagt acgaaatgac atattttccg aaagggtaca
    31501 cggatctaag attagaagga aagcagtgcc tttctcttca tattccttcc gatgcagatt
    31561 tatctcagaa taagataaga gatagttacg aaagtgccat tcgttttttt gaactttatt
    31621 ttcctgatta ccattaccaa gtgatgtaca cagatacttg gttactatca cccaatctta
    31681 ctaaatggtt gaaaaaagag tcaaaaatct gtctttttgc agccgattat cgattattat
    31741 cagttgatga acaggacgat agtggagtcc cttggatctt tggacgagta gatgcccaaa
    31801 tacatgatta tccagaaagt acgagtttac agcgacaagc aaaagagcaa ttgcttgcag
    31861 gcgagcacat tggctctggc ttaggtattc ttacttcata agtttagtac ttcgaaatag
    31921 tt
//
LOCUS       DACEOM010000032        31486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEOM010000032 DACEOM010000000
VERSION     DACEOM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.033"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(76..543)
                     /locus_tag="IW781_001390"
     CDS             complement(76..543)
                     /locus_tag="IW781_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1182394.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="IW781_001391"
     CDS             complement(663..2201)
                     /locus_tag="IW781_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1182395.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="IW781_001392"
     CDS             2281..2967
                     /locus_tag="IW781_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1182396.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IW781_001393"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IW781_001393"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1182397.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IW781_001394"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IW781_001394"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1182398.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="IW781_001395"
     CDS             complement(4453..5583)
                     /locus_tag="IW781_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1182399.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="IW781_001396"
     CDS             complement(5583..7766)
                     /locus_tag="IW781_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1182400.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="IW781_001397"
     CDS             complement(7763..8788)
                     /locus_tag="IW781_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1182401.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="IW781_001398"
     CDS             complement(8833..9660)
                     /locus_tag="IW781_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1182402.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10723)
                     /locus_tag="IW781_001399"
                     /pseudo
     CDS             complement(9657..10723)
                     /locus_tag="IW781_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(10749..11585)
                     /locus_tag="IW781_001400"
     CDS             complement(10749..11585)
                     /locus_tag="IW781_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1182403.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11582..12451)
                     /locus_tag="IW781_001401"
     CDS             complement(11582..12451)
                     /locus_tag="IW781_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1182404.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12521..13804)
                     /locus_tag="IW781_001402"
     CDS             complement(12521..13804)
                     /locus_tag="IW781_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1182405.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13818..15503)
                     /locus_tag="IW781_001403"
     CDS             complement(13818..15503)
                     /locus_tag="IW781_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1182406.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15778..16803
                     /locus_tag="IW781_001404"
     CDS             15778..16803
                     /locus_tag="IW781_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1182407.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17398..21051)
                     /gene="rpoC"
                     /locus_tag="IW781_001405"
     CDS             complement(17398..21051)
                     /gene="rpoC"
                     /locus_tag="IW781_001405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1182408.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21130..24747)
                     /gene="rpoB"
                     /locus_tag="IW781_001406"
     CDS             complement(21130..24747)
                     /gene="rpoB"
                     /locus_tag="IW781_001406"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1182409.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLDELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25475..26443
                     /locus_tag="IW781_001407"
     CDS             25475..26443
                     /locus_tag="IW781_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1182410.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26461..27366
                     /locus_tag="IW781_001408"
     CDS             26461..27366
                     /locus_tag="IW781_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182411.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27363..28169
                     /locus_tag="IW781_001409"
     CDS             27363..28169
                     /locus_tag="IW781_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1182412.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28326..29294
                     /locus_tag="IW781_001410"
     CDS             28326..29294
                     /locus_tag="IW781_001410"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1182413.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29611..30135)
                     /locus_tag="IW781_001411"
     CDS             complement(29611..30135)
                     /locus_tag="IW781_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1182414.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30422..>31486
                     /gene="scm"
                     /locus_tag="IW781_001412"
     CDS             30422..>31486
                     /gene="scm"
                     /locus_tag="IW781_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1182415.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8832 a         6800 c         5928 g         9926 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 tatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctccct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtgc acctgctttt ttagctaatt
    10201 gaatcgcaat ttgcccgata gcgcctaaac cgaagattgc tacagcatca cctgctcgaa
    10261 catgtgcttc ccggatacca cctaaagcga attgagcagg atcgtaacat actgcatttt
    10321 tccattgatc caaccgatca agtttacgta gtttataatt atcaacaccg tttgtaatca
    10381 gcgtttcacg aatgggtcca taagtgcaga ctaactctcc tttttgatac tctgtcacca
    10441 aactgcccgt ttcttcgatc gtaccgacaa tcatatttcc taattgaaat tcgccaaata
    10501 ccacaccgcg ttggcttcct tcttctcttg gcaagaatag gcgccattct tctgaaaatt
    10561 cttcatcgat aaatggactt aatcctctaa agtcgacaat ttctgttcct ggttttggag
    10621 tagcgaaaat tactcggata cgtacttcgt tcttttcgat ctcacggtct tcgtaatctt
    10681 tgaatccggc tattcttggt tctaatgcaa ctaatttttt catcttttct ttcctctttc
    10741 ttttcttttt atccttttac gccgccttcg actaaatcac ctttaataaa tcgttcagat
    10801 aacgcataca taatgatgac tggtaaagcg gtcaccaatg aagcggccat cattcgtccc
    10861 caaatgtagt ctggtgtact gaataatgtg ttcaaaccga ttggtaaagt aaatttttcg
    10921 gaggtcgaca ggaaaatcga agcaaataaa taatcattcc acgaaaccat aaagcaataa
    10981 acgaagacag aaatgattcc agaaatagcc aatggtataa tgatccgaat aatgatctgg
    11041 atccggctaa gaccatcgat cattgctgct tcttcaatat cagtcggaat cgtgtcaaaa
    11101 taacttttta gcatgaatat cgcagtagga agtgtttgaa cgaccatcgt gataatcagt
    11161 gctgtattgg tatcatacaa acctagaccg gaaatgattt taaataatgg aacaacgatc
    11221 aaaataccgg aaaacatata aacggtgtaa aagctagcat tgattttcga gcgagctttg
    11281 aagcgtagtt ttgacaaagc gtatgccccg ctgatcccaa tcactactgc agttacggag
    11341 gctatcaagg agatcactaa actatttttg aaatacatga tgaatggaaa aatctcagca
    11401 ttgaaaatat cctgataatg ttcaaaagtc cattctttcg gaaaaatagt ttgtgtcgtt
    11461 gcaattgctt cttgtgaatc cttgaatgat gtcatcagca tgatcaaaaa tggaaacaaa
    11521 ttgacagcta atagcaacaa aatcgtcgcg taaaataaga ttttcctgct ctttttattt
    11581 ttcatttcaa gtccaccttc tttcgtgaga gcaagacgac cgctgcaata atgatgaaca
    11641 atagaatcga tatagctgct gcttttccaa gatcattgaa cgcaaaggct gtctgataca
    11701 aataaacgcc taagatattg actttattcg tcagtagata gacatctgta aacatataga
    11761 acatccagat agatcgtaaa acaatcacag ttgctagtac tggtttgatt gccggcagtg
    11821 tcacgatctt gaacttctgc caagcatttg caccgtccat ttcagctgct tcataaagcg
    11881 ttttgtcgac tgtttgcaaa atagccaaaa atgaaataaa cgcatacgga tagtatctcc
    11941 agatagcaaa caaaacgacc ataaagaagc tagcaactgg ctggtcgaac cacagcggtg
    12001 cttcgttgaa aagctgcaac ctatctacta acagatcatt gacgattcca taactattat
    12061 tgaacatata tttccaagca aaaatcaaag aaatagatgg cgtaacataa cttaataaaa
    12121 tcagtgaacg caccgttttc ctaaatctga acttgcggtt aagcaaaata gctacggcta
    12181 atcctaaagc tgtactgcct gctacaacta agaatgtgta agcaactgtt aaacctaaag
    12241 aacgataaaa ttcactatca cttaaaattt caagataatt gcttaatccg ataaaaacgg
    12301 cgtctgagtt aggattcagc ggcatatcga aaaaactgat ctgaatattg gaaagcattg
    12361 gaatcagcac taaaacagct aataaaagca tgcttggtgc taaaagtccc atggctaatt
    12421 ttaagtcaga atgtttcctt ggacgattca ttgaaacctc cccctgataa tctgccaata
    12481 gctatctact tttttcgaac accattggca gaacttcttg ttatttcgta tttttttgtg
    12541 cgtcagctaa agcttgatcg acatcttcgt ttccgacagt tacttggttc actgcttttc
    12601 cgatcacacc tgagcttgtg atatctccca ttttcgtgaa attcttttcc ccgactagtc
    12661 caaagacttg tacttcatca aaagcactag caatctcaga tggcagctcg ccaaaagact
    12721 tgatgacttc attttctttg taaccatcca gttcgactac ttgtttattc actggttgag
    12781 caccaccagg agacatcaag acccactttt ccatgttttt cggttgagat aagaaagctg
    12841 tgaatgcttc agcagcctct ttttgtttct cgtctagacc agaagaaatc gtcaatccag
    12901 atactgtgcc atagacagct tgatcttttt gtgttgggat agcaaaaccg atattttctg
    12961 agttccccgc ttcataaaca gaaggcaaaa tgtaagttga atagattgcc attggtgctg
    13021 ttccattcat gaaagcatct ttgatttctg tcacatcatt agagcctggc atcgtgtatt
    13081 gggataagtt ttgataaaac gacaatgctt ctctcatctt ttccgtatcg atcgttacct
    13141 cgccttcatc atccaaaaca tttgctccat ttgacaaagc aaattgagag aacgcttgct
    13201 cactcattgt tccttcagca gtaggcatag caattccata ttgtttattc tctttatctg
    13261 tgaaatgttg agcaattttt aaaatatcat cccaattttc tggttctgaa aaaccagctt
    13321 ctgataattt ttctttgtca taccaaatac cttgaaccca gccgctaatt ggtgcagcaa
    13381 tatagctttt tccatcttct gaacgaacaa ggtttttggc tccgtcataa taattatctt
    13441 ctccagcttc ctcgataacg ttttggactg ccgtctgatc aatcagttca tctttatcca
    13501 tcactttagc aaaatcctga ctgacttcca ttacttctgg taatttaccg gaacgagcta
    13561 acgtaacgat ttttgtattg aacgctgatt cttctaccgg cacttgcttg attttgatgt
    13621 ctggattttc tttttcaaaa tcagcaacca gttcattgat cacacttaat cgttcctgtt
    13681 cgacagaaga gtgcatgaat tgaatcgttg tctgttgtga ttcgttagag ctttgacttt
    13741 caccacaagc agtaagaaga aatccagtag ctagcagaca ggtagtaaca ataatttttt
    13801 tcattttttt ccacctctta ttttattatt ttcagccaca tatattcata cggctggaat
    13861 atcaactttc gttcttgact gcaatggata atttgtccag aaaacaggtc taggtaatct
    13921 ccttttggta aaagataacc tgctgattga ttggaaaaat tatggataac taagactttc
    13981 tcttccttcg cttcgcgaat aaaactgata aaagaatctg ctgagtccag taagtaaatc
    14041 aaactattcg gatggaaggc tttttctttt tttcgaatag atagcatctg ggtcaattca
    14101 ccatatactg ctgttcttaa tggatctgtc tgcaactctt tctcaatatc tgctaacaaa
    14161 aatttttgcc gattgatttt tcgattctct cctgtttttt ctacgccttt gtaatcattt
    14221 cgacttccca gcacactttg aatataaatt gccggcacgc ctggtaacgt taccaaaaat
    14281 gaatgagcag ctaaaaagcg tttgcttctc cattccgtcg tatctgttgg agaacttaac
    14341 gcatctagat acgtagcgtt tatctcatat ggactggtcg ttccatctgg gtttttcttg
    14401 tagtttactc gggcaccctc tttcttcagc tgttccacta attcaaggat cgttttttcc
    14461 ggcagaatac ctcgtaccgg attcaatccg ataccgtcat gggaggctaa aaaattaaaa
    14521 aaagttgttt ttttcgatgc gaaatcgatg gtttttaacc attctttcaa cttacttgta
    14581 ttacccgaac gaatcgcttg aagggtcaaa ggtggtagcg aaaactgata gaccatctgt
    14641 gcttcattct cgccattccc aaaataactg atattatctt tgtgtggcac atttgtctca
    14701 gtgatcaata ccgttcctgg agctgcttca tcagcgattg cacggaataa ttggatgatt
    14761 ttatgcgttt ctggcaggtg tatacaagaa gttcctagtt ctttccataa aaatcccaca
    14821 gcgtccaagc gaataaatga agcgcctttt tctaagtaaa acaaaagaat atcgatcatt
    14881 ttcaataaaa cttctggatt tttaaaattc aagtctactt gatcttcgct aaaagtcgtc
    14941 catacgtgtt tttctttccc gtttttgtca atgaacgttg taagtaacgg gctggtcctt
    15001 ggtctagtca cgctgcttgt atctgtatcc agtggcaaat caataaaaaa atctttgtac
    15061 gtatcattca agtttaaata ttcctgaaac caagtacttt cggcagacat atgattgcag
    15121 acaaagtcga acattaaccg tgtttcgctt ctcatttctt ctatatcttc ccatgtgccc
    15181 aaacggtcgt tgattgcctg ataatttaca acggagaaac catcatcaga tgtatatggg
    15241 aaaaatggca agaaatgtac aactctaaat gtatctgcca aatacttttg ataaaatttc
    15301 ttaaatgtac gcaaagttgg ttgctgctct tcaaaaaatt gatccccata agtaatcaag
    15361 aaaatatctt cttcgtccca atgcagtttg cctgcctggc ctttcttctg ctgataaaca
    15421 ctgacttttt gttcaatcat agaggcggtt ttctcagaca aatctgagtc gcgataaatc
    15481 gcttgtaaaa gttttgtata catcggattc ccctttcttt tttgggagcg ttcccaacga
    15541 tttgatcata ttatggaaaa cgcttactgt caatagcatt tttttaaata attttgatat
    15601 acacaaaaat acaaataaga aagcttatgt atcaataatt aatagcttct taaaaaaata
    15661 tggtctctca tgatcatgca agctattcct aaattttcaa aatcatagac aaaaaagaat
    15721 gtctattgta ctatggaatt attctaaaac gctcccaaaa aggaggtgca tataaaattg
    15781 aaacctacta tttatgacat tgcgaaaaaa gctaatgtct caaaatcaac tgtctccaga
    15841 gtattaaata accagccaaa tatctctgat aaagcaaaag caagtgtact tcaagcaatc
    15901 aaagaattga attatgaacc gagcaaaatt gcaagaggac tgtcaggaaa tggttttgac
    15961 gcgattctag cattatctca tcgtacttct catacgacct atggcaatcc ttttttttca
    16021 gatgttattc aatccatttc tgccgtttct gaacaaaata acttcgactt gattctacaa
    16081 acggcaaaaa caccagaaga agagctagaa aaatgcgcga aaaagatcca agaaaaaatg
    16141 atccgaggaa ttattatttt aagctctacg atggatgaaa cacttctttt tgaattggat
    16201 caatacaata ttcctattgt ggtaatcgga aaagtcggaa acgactacac gaacatctac
    16261 tcagtggaca cggataattt ccaggatagt tacgatttgg tggaaacact gatcaagaat
    16321 ggacaccgtc atatcggctg tctccatgct ccgacagaag tttatgtatc caatgatcgt
    16381 ctcaatggtt accgtcaatg cttatttgat caccacctag atatccgtaa tgaatggatt
    16441 atagacggcg gctttcgctt agaagatagc atggcagcaa tgaatgattt aatgaagcta
    16501 cgagaaagac caactgcgat tttttctacc gatgcattaa aatcattgag tatttacaaa
    16561 gtggcagaac aatctggaaa gcagatacct gatgatcttt cgatcgcagg attcagtgat
    16621 cccttattgt cctccctatt ttcgccacag ttgactcgga tagacattcc gacaaatcag
    16681 ttaggaatca aagccacaga acttttattc caactaatcc accaccaata ccctgaagaa
    16741 aaaagaatca ttctttcaac cgagattact ctcacagatt cgatccaaca acaagaaatg
    16801 tagaacagaa ttataaaaga acagaaacac tgaggataag tcagttactc tgttcttttt
    16861 tcaatcactc atccctcgtt accccctccc ggtattttag cgaatttttc ttattttcca
    16921 tctgttttag atagctgtgg ataactataa tgtttgtgtt aaactatttt ttccacaggg
    16981 aagaaaaaac tatttttaac cacgttgtgt ataagttttt atagttcgtt tttttaaaca
    17041 catttaccct tatttcaatc catgttattt ggggatactt atctctaaaa cataaataga
    17101 ataacagaca tgtctactgg ttttgtttta ttcgaggaat tattcaataa tagctagcaa
    17161 aatattaaaa ataaccgctc tatcttccca tcaatttatt ttgcattcaa aaaatgatct
    17221 atctttactt ttgttgattg acttctcttg gaagatgatc gttccgatac cttaattgga
    17281 tacgaaccga tgttgttaag atcctttccc acttttcttc tagttagaca aattatttta
    17341 gacaaaccaa aaaagaggtt gtgacaataa tttgtcacaa cctctttttt aaaatatcta
    17401 ttttgtttga ttgttcattg cgtcttctgc agccatttgc gcttcaatat ctgaaatact
    17461 gtagacattt tcgcttgcta caccgacttc ttttggttcc atgtcacgat aacgttgcat
    17521 accagtacca gctgggatga tcttacctat aataacattt tctttcaaac caagaagagg
    17581 atctttctta ccgcggatcg cagcatccgt caagacacga gttgtttctt ggaatgatgc
    17641 agcagataag aaactatttg tttctaatga tgcttttgtg atacctagta agacaggtcg
    17701 agaagttgca ggaacgcctc ctgagactag cgtattgtag ttttgatctt taaagtctgc
    17761 gatgtctaat aatgtacctg gtaagatatc tgtatcacct ggatccatta cacgaacttt
    17821 acgcagcatt tgacgaacca ttacttcgat atgcttgtcg ccgatttcta ccccttgcat
    17881 acggtaaaca cgttgtactt cacgaagcaa gtagttttca acagataata catcgcgaac
    17941 ttgcaacaat tgttttggat cgattgatcc ttctgtcaat ggtgcaccac ggtggatcat
    18001 atcgccttct gctactttca tacgtgctgt ataaggaaca gtatacgtac gtgtatctgt
    18061 ttttcctttg attgttactt ctttggtacg tgttgcagga tcttctgaga tatcgatgac
    18121 atcccctgtt acttctgtga tcactgcttg ccctttcgga ttacgtgctt caaagatttc
    18181 ttggacacga ggaagacctt gagtgatatc gtctccggca accccacctg tatggaatgt
    18241 acgcatcgtc aactgagtac caggttcacc gattgattga gcggcgatcg taccaactgc
    18301 ttctccgact tcaacgtcag agccagttgc aaggttgcgt ccgtaacaat gtttgcagac
    18361 accatgtttt gtattacatg tgaagacaga acggattgtc actgtttcga cacctgcatc
    18421 aacgatttgt tttgcgatgt cttcggaaat caattggttt cctgcaatga tcaattcatt
    18481 tgtttcagga tgacgtactt ctttacgggt gtaacgtccc actaaacgat cttctaatga
    18541 ttcgatgatt tcatttccct cgcggatggc ttggatttca agacctcggt cagtaccgca
    18601 atcttcttca cggatgatta catcttgggc cacgtcaacc agacgacgag tcaagtaacc
    18661 tgaatcggca gtcttcaagg ctgtatcggt catcccttta cgagccccgt gagttgagat
    18721 aaacatttcc aagacggata gtccttcacg gaagttcgaa atgattggca attccatgat
    18781 tcgaccatta ggagcggcca tcaaaccacg cattccggca agctgtgtaa agttggaaat
    18841 gttaccacga gctccagaat cagacatcat gaagattgga tttttcgctt ccaaaccttc
    18901 catcagtttt tgttggatgc tgtcttttgc agcattccaa acagcaatga cacgttcata
    18961 acgttcgtca tctgtgatca gaccacgacg gaattgtttt gtaatagttt caacttgttt
    19021 atgggcattt tcaatgattt cttgtttttc attcagtaca ctgatgtcag cgatccctac
    19081 tgtgataccg gcatgtgtcg aatgtttgta ccctaagtct ttcatacggt caagcatctt
    19141 agaagtttct gttactttga atcgtttgaa gacttctgcg atgatgtttc ctaagttttt
    19201 cttcttgaac ggtaatacta attcttgctc tttgatatgt gcaggaatat cagtaccagc
    19261 ttcaacaaag tatttgtccg gtgtttggac ggtcaagttg aagtctgttg gttcattcag
    19321 ataagggaat tcaggaggca tgatctcgtt gaagatgatc ttaccaactg ttgtgatcat
    19381 cgtacgttct ttttgccatt cagtaaatgg tttttcacct aatgagttag ggttcacacc
    19441 gatacgtgaa tgtaaatgaa catatccgtt gcgccatgcg ataacggctt cgttttgatc
    19501 tctgaagacc attccttcgc cttcgcgtcc ttcttcttcc attgtcaagt agtagttacc
    19561 tagaaccata tcttgagaag gagtaacaac tggtttacca tctttagggt tcaagatgtt
    19621 ttgagcagct aacatcaaca tacgtgcttc tgcttgtgct tcttcattca atggaacgtg
    19681 gacagccatt tggtctccat cgaaatcggc attataagct tcacatacaa gtggatgcaa
    19741 acggattgcg cgtccttgta ctaaaactgg ttcgaatgct tggataccaa gtcggtgaag
    19801 cgtaggtgcc cggttcagca atactgggtg ttccttgatt acgtcttcta aaacatccca
    19861 tacttcgtct tcttgacgtt cgattttacg tttcgcattt ttgatgtttg aagctagttc
    19921 gcgttgaaca agctcacgca tcacgaatgg tttgaataat tcaatggcca tttcttttgg
    19981 aagtccacat tggtacatct tcaagaaagg tccaacaacg ataacggaac gacctgagta
    20041 gtcgacacgt ttacctagca agttttgacg gaaacgacct tgtttccctt tcaacatatg
    20101 agaaagagat ttcaatgggc ggttacctgg tccagtaact ggacgtccgc gacgaccgtt
    20161 atcgatcaat gcatctactg cttcttgcag catacgtttt tcattttgaa cgatgattcc
    20221 aggtgcattc aagtctaaca aacgtttcaa acggttatta cggttgatta cacgacggta
    20281 aagatcattc aagtcacttg ttgcaaaacg tccaccttct aattgaacca ttgggcgtaa
    20341 atctggtgga ataacaggga taacgtccat aaccatccag cttggttgat ttcctgaagc
    20401 gcggaatgct tctaaaatat ccaaacggcg gattgcacgt gtacgttttt gtccttgtgc
    20461 agatttcaat tcttctttca attcggctgc ttctgcatct agatcaacat tatcaagtaa
    20521 ttgtttaatc gcttctgcac ccatagcagc ttggaattct tgtccatatt gttcacgttt
    20581 ttcgcgatat tcgcgttctg tcaacaattg ttttttctct aaattcgtat ttcctggttc
    20641 gatgactaca tatgaagcaa aataaatgat ttcttccaaa gcgcgtgggc tcatgtctaa
    20701 tacaagaccc atacgtgaag gaatcccttt aaagtaccag atatgtgaaa caggagctgc
    20761 taattcaata tgtcccatgc gttcacggcg aactttcgaa cgagtaactt ccactccgca
    20821 tcgatcacaa acgattcctt tataacggat acgtttgtat tttccgcagg cacattccca
    20881 gtcttttgta ggaccaaaaa tccgctcaca gaacaaccct tcgcgttcag gttttaaggt
    20941 acgatagtta atcgtctcag gtttttttac ttcaccgtaa gaccagcttc tgattttttc
    21001 gggagaagcc aaacctattt gcatactttc gaatttattt acatcgatca aaaggggttc
    21061 cctccatttc atttaatggc aaaccgcaag acggctgatc ttcgaccgcc ttgcagctca
    21121 tagaccggat tagtctaaat tatcttctgc ggtttcaaag ttttggatga catcgtcttt
    21181 ttcatcttcc acttgttcag cagctttctt ttctaattct ttggcagttt gctgttcggc
    21241 aaattttgtc aaagcatcaa cagtgatcaa atcgtcgtct tcatcgtcca tgtcgcgaag
    21301 ttcgatctct gtttcttcga tatccaagac acgcatgtcc agacctagtg attgcaattc
    21361 tttcaccaat acgcggaatg attccggtac acctggtttt ggaattggtt cacccttgac
    21421 gattgcttcg tatgttttca cacgtccgac tacgtcatct gatttgtatg tcaagatttc
    21481 ttgtaaagta taagccgcac cgtaagcttc cagtgcccat acttccattt caccaaaacg
    21541 ttgtccacca aattgagctt tacctcccaa tggttgttgt gtaaccaatg agtaaggtcc
    21601 gattgaacgt gcatgcaatt tgtcatcgac catgtgagca agtttgatca tgtacatcac
    21661 accgacagac acacgtccat caaatggttc acctgtacgt ccgtcgtaca gaacagtttt
    21721 cgcatcgctg gccataccgg cttcacgaac tgtttcccat acgtcttcat cacttgcacc
    21781 atcaaatact ggcgttgcta cgtggatacc taactgacgt gcagccatac ccaagtgtaa
    21841 ttccaatact tgcccgatgt tcatacgtga aggcacccct aaaggattca gcatgatatc
    21901 gattggtgtt ccatcaggta ggaatggcat atcttcttcc ggcataatac gggatacaac
    21961 ccctttattt ccgtgacgac cggccatttt atccccttca tggattttac gtttttgaac
    22021 gatataaaca cgaactaaca tgtttacgcc tggtgacaat tcatctccag cttcacgagt
    22081 aaagattttc acatcgtgaa cgataccgcc gccgccatgt ggtacacgta atgatgtatc
    22141 gcggacttca cgagcttttt cgccaaagat cgcatgcaat agacgttctt ctgcagataa
    22201 ttctgttacc ccttttggcg tgactttccc tacaagcaaa tcgccatctt ggacttctgc
    22261 accaatacgg atgataccca tttcgtctaa atcttttaat gcgtcttccc cgacattagg
    22321 gatttcgcgt gtgatttctt caggtcctaa ttttgtatcg cgggcttctg attcatattc
    22381 ttcaatatgg atagaagtgt atacgtcatc ttttaccaaa cgacggctca tgatgatcgc
    22441 atcttcatag ttgtaccctt cccaagtcat gaaaccaact aaaacgtttt ggcctagagc
    22501 catttcgcct tgttccatag atggtccgtc tgccagagta tccccttttt cgactttctc
    22561 acccaaatga acgattggac gttggttgta gcttgttccg gagtttgaac gacggaattt
    22621 tgttacatcg tatttatcta atgcaccgtt gtcgcggcgt acgcggattt cagaagcatc
    22681 aacgtattca acgacaccgt catgtttaca taacaaagca gctcctgagt catgcgctga
    22741 tttgtattcc ataccagtac caacccatgg tgcttgagga ttgatcaacg gcactgcttg
    22801 acgctgcatg ttggcaccca tcaaggcacg gttggagtca tcgttttcca agaaaggaat
    22861 acatgctgtc gcaacggcaa ctacttgttt aggtgatacg tccatgtagt ctactttatc
    22921 aatagatact tctaagtttt cactttgagc acgagccata acaacgtctt gagcaaatgt
    22981 tccatcttca ttcaatgggc tgtttgcttg ggctacgatg taatggtctt cgatatcagc
    23041 tgtcaagtag tcgatttggt cagtaacacg acctgtttca cggtctacac gacgataagg
    23101 tgtttcgata aatccgaatt tatttacttt cgcataactg gacaaactat tgatcaaccc
    23161 gatgtttggg ccttcaggag tttcgatcgg acacatacgg ccatagtggg agtagtgaac
    23221 gtcacgaact tcataaccgg cgcggtcacg agtcaatccg ccaggtccta aggctgaaag
    23281 acgacgtttg tgtgtcaatt cgtcaagagg gtttgtttga tccatgaact gtgacaactg
    23341 agaagaacca aagaattctt tgatacttgc cacaactgga cggatattga tcaattgttg
    23401 aggtgtcaat gtttcagtat cttggatcga cattctttca cgaaccacac gttccatacg
    23461 tgctagcccg atacggaatt ggttttgtaa aagttcaccg actgaacgga tacgacgatt
    23521 tcctaagtgg tcgatatcat cgacattgcc gataccttcc atcaagttca agaaatagct
    23581 cattgaagca atgatgtcgg ctggacgaac cgtttttaca gctgcttctg gatagccgtt
    23641 tccgatcacg tttacttcac gttcagggtc tcttggtgaa aatactttga tgacttggac
    23701 agtcattgga tctgtgacca caccgtcttc tgatggatag taagtcactg aatttaggcc
    23761 attatcaatg aaaggagcta gtgtttccat gacttggtga gtcagaactg ttcctttttc
    23821 aacgatgatc tcgcctgttt ctggatcaac taatgtttca gctaatgtca gattcaatag
    23881 acgtgttttc aaatctaatt ttttgtttac tttgtaacga ccaacgtttg ctaagtcgta
    23941 acgttttgga tcgaagaaac gagcattcag caagttacgt gagctgtctg ctgtttttgg
    24001 ttcgcctgga cgcaaacgtt cgtaaacgtc tttcaaacct tcttctgttc ttgagtcgct
    24061 tgcgttttta tgcaagtctt tttcgactgt attgcgtaat gtttcgctgt cgccgaagat
    24121 ttcaaagatc gtatcatcgg aaccaaatcc taacgcacga actaatacgg ttaaaggaat
    24181 cttacgtgta cggtcgatac gtacataaga gatgtctttt gcatcagttt ccatttctag
    24241 ccaagctcca cggttaggga taacagtaga accgaatcct tcttttccgt ttttgtcgac
    24301 ttttccatgg aaatagacac ctggtgaacg gactaactga gaaacgatta ctcgctctgc
    24361 cccgttgatg atgaatgttc cttgttctgt cattaatggg aaatcaccga agaatacttc
    24421 ttgtgctttg atttcgccag tctcgcggtt tgtcaaacgc aatgttacat gtaatggtgc
    24481 agagtagttt gcatcatgcg cacgagcttc tgctactgta tactttggtt ctttcaattc
    24541 ataatctaca aattccaaag atagattgcc attgaagtca tcgattggca aaatgtcttc
    24601 aaacatttct ctaagtccct catctaagaa ccattggtaa gagtctgttt gaatttcgat
    24661 caaatttggt aattccaaca cttcactgat tcttgcgaaa cttctacgtt cgcgatgttt
    24721 tccgtatttt actacgtgtc cagccaagct cttcacccct ctaaaatgtc attcaaaaag
    24781 actacccgat caattgttac aataatattt caaatattat ttttaggtaa caacagacat
    24841 tttttgcggc ttttaggcaa aaaaaaacca aaaatagaac attctcttga aaccttactt
    24901 cgtgagcttc tacttttgtt tccttatatt aaagccgaaa cggacaatat ttcgtttctg
    24961 ccttttcttc atcagatagt ttttgcaact tcatatacta cacttatttt ctataaaagt
    25021 caacacttcc tggcgtttct tctgcttttt cgcaacattt tcttaatgtt ttcaatttta
    25081 tgttttcaat tatctgacaa ttcttaattg acagaccttt ttatctcggt tatactttag
    25141 aaaattcatt ttgtggagga acttctatgc gaaccaacat caaccaaatc gcaccaacac
    25201 aactgaataa cttttacttt agctacttta gataggtttg tattcatcat gatggataca
    25261 aacgttaaac gacagtttgt atctatgatg gctaaagaac tttgtattgc agcaaaaagc
    25321 cacgtagaca aaatgctaag tggctgagca gtgaaaatac aaagtaaaag tatcaagcat
    25381 tctacttttt caatttattt tccgactttt caatccattt ggacattttg tctgaatgga
    25441 ttttttgttt cccaaaatta aggaggaaaa agaaatgaaa aaaaatataa tcatcggatt
    25501 aggcgctgcc ctgctactag ctttaggagg ttgcgcacaa aaagacaatt ccgcagaggc
    25561 atcagatgga caaaaaacaa ttggcgtatt gcaacttgta caacataact cgttagatgc
    25621 tgcctataaa ggattcaaag aaggattagc agaagaaggg tataaagaag gcgataatct
    25681 aacaatcgat tatcaaaatg cccaaaacaa tcaagacaac ctgaaaagta tgagtgagaa
    25741 actcgtcaaa gaagatcctg atttgctact tggaatcgcc actccagcag cacaatcttt
    25801 attaaatgaa acaaccacta tcccgatgac tgtcacagcg gtcacagact tggaacaagc
    25861 ccatttagtc acatccaatg aaaaacctgg cggcaatgtg actggcacga cagatatggt
    25921 gccaatcgaa aaacaaatcg gcttactgtt aaatatcgtg ccagacgcca aaacaatcgg
    25981 catcatgtat aacaacggtg aagcaaactc taagatccaa gctgatttgg cagaaaaaga
    26041 attgaaaaaa gcgggcgtca aagtcaaagt actgacagca aattccacaa atgatgtcca
    26101 acaagtgacg aaaagtctag ctaaagatgt agatggaatc tatattccta ctgataacac
    26161 gttcgcaagt gcggcaagtg ttgtcggtga agtcgcaaaa gaaacaaaaa cacctgtcgt
    26221 tgctggcgca gttgatcaag tcaaagacgg cggtctagca acagtaggaa tcgactacga
    26281 aagcttgggc aaacaaacag ggatcatggc agcaaaaatc ttagacggca agaatccaag
    26341 tgaacttcct gtcgaaacag cgaatgattt ggaactgtat gtcaataaag acatggccaa
    26401 agcattaggc atcaatccag acagcatcaa agaagctgaa taaaacacag gaggattctc
    26461 atgacaaatt tgctctctct tttcttatca tctgcatcgc aaggggtctt atgggcccta
    26521 cttgcgattg gtgtatacgt cactttccgt atcttggaca ttgccgactt gactgctgaa
    26581 ggaagctttc ctttaggtgc agggatcgcg gcagtaagta ttacaaatgg ctatcatcca
    26641 ttgacagctt gtcttctagg ctttttaggg ggtgcggcag cagggcttgt ttccggactg
    26701 cttcacacaa aattgaaaat tcccgccctt cttgcaggga tcatcaccat gactggttta
    26761 tattctgtca catcccgtgt gatgggtgcg ccgaacatct ctttattggg tcagccaacg
    26821 atctttcatt gggtcgagtc attcggcata acaaaagaca acgctgtact gatcgtcggt
    26881 ctgctttttg ctttgatcgt tgtatgttta cttgttttgt tcttacgtac agaaacagga
    26941 cttgctattc gtgcaactgg cgataatctg gcaatgagcg aagcaaacgg gatcaacaca
    27001 gataatatga agatcatcgg ttatatgatt tctaatggat gcattgctct ttctggctcc
    27061 ttgcttgcgc aaaacaacgg ctttgctgat ttaaattctg gtattggaac gatcgtaatt
    27121 ggacttgctt ctattatcat cgcagaagtt ttgttacgta accagccact atttctccgt
    27181 ctcttgactg tcgttttagg tgcggtcatt tatcgtttta ttttagcgtt agtcttcgaa
    27241 ctgaatgttg aaccaagtga ttcgaaactt gcttctgctt tcgtgctagt gatctgtctt
    27301 tctttcccgc agatccagca aaaattccat ttacctcaaa aaaagaaagg cggtagtgtc
    27361 aaatgaatga cgtcttaaca ttggaaaatc tccatcatac ctttgaaaaa ggaacgatca
    27421 acgaaaatca tgtcctgaaa ggcattaatc tttccttaga tcccggcgac ttcgtgacga
    27481 tcatcggtgg aaatggggct ggtaaatcta ctttgctgaa cagcatcgcc ggtacatttt
    27541 ctgtcgatca gggtcaaatt ttattgaatg gaaaaaatat cacgaaaaaa tctgttgtcg
    27601 aacgttcgaa atccatcagt cgcgtattcc aagatcctaa actagggaca gccgttcgtt
    27661 taacggtaga agaaaatctt gctcttgcta tgaaacgagg aaaaaaacgc ggcttcttcc
    27721 gtggtgtcaa accacaagac cgaacatttt tcaaagacca gctggttcgg ctaaatttgg
    27781 ggttggaaaa tcgtttatcc tcagaaatcg gtttgctgtc tggaggacaa cgtcaagcta
    27841 ttacgttatt gatggccaca ttacagcgtc cagaactgat cttattagat gaacacactg
    27901 cagcgcttga ccctgctact tccaaaacgg tgatggaatt gacagaaaag ctgatcactg
    27961 agcagaatct cactgctttt atggtcactc atgacatgga agacgcgatc cgctatggga
    28021 gtcgactgat catgctgcat caaggaagga tcgtcgtaga tatccagcaa gaggaaaaac
    28081 agcagctcac agtgccccaa ttattagcgt tgttcaaaca aaacagcggc acggcattaa
    28141 aagacgatca agtactttta tcttcataat cagaaagagg atgggacaaa aaatgaacgc
    28201 ccaccctctt cttttttctc tttccttctt tcgtttttgc tttatgtatc aaccgttgtt
    28261 tcttcgaaaa ctattttgtt ctctccttct tcctgataga atggatggga aaggagcttg
    28321 atgccatgac aacgaaagac aaggtacttc aattattgaa aaagacacca gattttcttt
    28381 ccggagaaaa gttggcacag caactggaag tttcccggac aagtatttgg aaagccatca
    28441 aagaattgga aaaggcaggc tatcgttttg agcacgggcc tacagggtat cgttacctgc
    28501 cctctgacgt actagatgca aaagaaatcc agcaagagct acggtcgttt attcctgatt
    28561 tgaaagtaca aacgatgttg acttctgaat ctacgatgaa agatgccaaa ctggcagcag
    28621 ctcaaggaga ttctgtttcg aagttatttg ttgctgatac acaagaacaa gctcgtggac
    28681 gttttggacg gccttttttc gctgaacctg gacgaggaat ctacatgagt ctcttgctcc
    28741 atccagacaa acggtttgat gaattgcctc aatatacgat catcgcagcc agtgcctgtg
    28801 tgaaagccat tgaatctgta accggtcgtt ctgcttcgat caaatgggtc aatgatatct
    28861 acatggatgg aaaaaaagtc tgtgggattt tatctgaagc agtcagtgat atggaaagcg
    28921 gcaggatcag tcatatcatc attgggatcg ggcttaattt ttctgttcct caaaatcatt
    28981 ttccagctga tctccaagaa aaagccatct ctgtctttcc cgacggacaa gctagtgtca
    29041 caaggaatca gctaatcagc aaaatctggc aagaattgtt cacttatctt tctcacccag
    29101 aaaaagcgtt ggacttctat aaagaaaaat cattcgtatt gggaaaaacc gtgtcgttca
    29161 atcaaaaagg aacgacttat gtcggtcttg caaaagaaat cggtgatcat ggcgaactga
    29221 tcgtccaaac aaatgaacag aagatgagtt tgtcttctgg cgaaatcagt ctcacatcta
    29281 tccgtacgca ataattatca gatttcacaa aaggacacct gatctcatac agtgtccttt
    29341 tttctgaatt ctcttcaaaa gactctcctt attctatcag ctgacagttt ctctatcctc
    29401 tagaaaaagg tattctaaaa atatctataa agcatggtac actttcttta aatgaatcaa
    29461 tgcaaaaaac aagagagtca tcagtttaca cgatcaaaag agatagcagg ccgcttcctt
    29521 tttcaatctt atagatagac catgggtaac gataaaaaca aaaaagaact tgcgacttat
    29581 tttgccgtaa gttctttttt agctagtgcc ttaatctctt aatcccaaca aacgtctcgc
    29641 atagatcacc tgaccagagt gcatagcagc atcatcaata atagaaacga gacgcattcc
    29701 tcgtgtgact ggtggtgtcc agttctcgtc aattacttca tctagctcgg aaggttccag
    29761 ccttcttaag taagaaatag caaaatcagc agctcctcgc aagtatccta gcagatcttc
    29821 tatttgatta gcttggatct gctttgcttg ttccagacta tgcttccaat cttgttcgtt
    29881 atctgctatc gtcatcggaa accggctttc ccagtcttga ctatgccaaa ttggagaagt
    29941 tcctgctaaa tcagcaatct ggaaatctaa ttctcgagcg gtatgccaag ccagccaagt
    30001 cattgatttg atctgcgggg ctctatcttc cactggaaaa cgattggcct cctcaataga
    30061 gacgccttca aacaaacgtt caaaccgatc gtatgcccga tccacactct cgcatagcat
    30121 atctaaataa tccatttatt ttcctccttt ttatttatcc tacttacttt ttcctaattc
    30181 taggatacct acttatatgt tactacacac ccttattttt tacactttaa cagtcaccat
    30241 catcaatagt ttggagattg aaagaaaaag atagtgtcga actatcaatt gaacaagaag
    30301 caacaatatg aacgtttcat taatattctt taaataaaca atgatttttc aaacgaacag
    30361 tttaagacta ggagaaaaag agtataatag gaaaatgttt gaaaggaaag gaaggggaaa
    30421 aatgaaaaaa agggcaagtt tactagtcct agttgctttg ttgttcaata tcttttcgta
    30481 tggtgtggcc gtttcagcgg aaacatacat tcctaaagac cctgggaata tttataatga
    30541 tctaactggt aactatggct tgttaggaat tgctagtcaa ttccacgtat ttgctaaaaa
    30601 taagacaact gtgaatgcac atacagacgg aaatatagca acacatgaac ttgatgcaaa
    30661 aaataatttt ggtacagagc ttcatgaagg tttattgaac caagaaataa attatataca
    30721 aaaaactgat tctttcgcta attcttcagg catacctaca ggtagcacaa gaatgaataa
    30781 atttgtcgtt ggcagtaacg tggatgtagc acttcaagat ggtcatccta cagtcaatgg
    30841 atccaggctt gatcacttaa ctccagatga tttttataaa gatcgtgctg ggcatgaata
    30901 tattgatttc gagcaggaat ttgctaaatt gaatgtagca gcaaatgatc ttgcaacgat
    30961 cactccagct aaaacatata ctgctgcaga ttttccagac atgaacaatc ggacaatcga
    31021 tttaactgat ttatcagata ccgggttttt attggtaaat atcgatgcag aagtgttaac
    31081 gatgaatacg ccacttcaaa tcattaatcc aaatgatcaa gtagttgtat ttaatgtcat
    31141 caacagttct tctgcattga acgtacagtc accaatcaaa tacaatgatc gttcaaatca
    31201 tgaaacagaa gatttttcgg atgcgaatat ctcttggaat tttggaaatg agatgactga
    31261 tttgacaatc agtgctcctt ttcaaggaac aatccttgcg cctaatgcta ctatccgcat
    31321 tactcaaaac caagacggaa cgattatagg aacaaatgtg attttagatg cagctaccaa
    31381 tcgttgggat cctaacgaaa tatttatcag cgacaatggt acagatacga cagacacaag
    31441 cgacagtaca gatacatcta ccacaagtga tagctcagat tcatca
//
LOCUS       DACEOM010000033        31261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEOM010000033 DACEOM010000000
VERSION     DACEOM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.034"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(371..3649)
                     /locus_tag="IW781_001413"
     CDS             complement(371..3649)
                     /locus_tag="IW781_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1182416.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3920..4465)
                     /locus_tag="IW781_001414"
     CDS             complement(3920..4465)
                     /locus_tag="IW781_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1182417.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4477..5169)
                     /locus_tag="IW781_001415"
     CDS             complement(4477..5169)
                     /locus_tag="IW781_001415"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1182418.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(5188..5472)
                     /locus_tag="IW781_001416"
     CDS             complement(5188..5472)
                     /locus_tag="IW781_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182419.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5485..6048)
                     /locus_tag="IW781_001417"
     CDS             complement(5485..6048)
                     /locus_tag="IW781_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1182420.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6276..6938
                     /locus_tag="IW781_001418"
     CDS             6276..6938
                     /locus_tag="IW781_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1182421.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            7062..8258
                     /locus_tag="IW781_001419"
     CDS             7062..8258
                     /locus_tag="IW781_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1182422.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8359..9735)
                     /locus_tag="IW781_001420"
     CDS             complement(8359..9735)
                     /locus_tag="IW781_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1182423.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9772..10638)
                     /locus_tag="IW781_001421"
     CDS             complement(9772..10638)
                     /locus_tag="IW781_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1182424.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10727..11752)
                     /locus_tag="IW781_001422"
     CDS             complement(10727..11752)
                     /locus_tag="IW781_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1182425.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11770..12444)
                     /locus_tag="IW781_001423"
     CDS             complement(11770..12444)
                     /locus_tag="IW781_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1182426.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12628..13521)
                     /locus_tag="IW781_001424"
     CDS             complement(12628..13521)
                     /locus_tag="IW781_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1182427.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13595..15292)
                     /locus_tag="IW781_001425"
     CDS             complement(13595..15292)
                     /locus_tag="IW781_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1182428.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15285..16598)
                     /locus_tag="IW781_001426"
     CDS             complement(15285..16598)
                     /locus_tag="IW781_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1182429.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(16598..20635)
                     /locus_tag="IW781_001427"
     CDS             complement(16598..20635)
                     /locus_tag="IW781_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1182430.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(20648..21106)
                     /locus_tag="IW781_001428"
     CDS             complement(20648..21106)
                     /locus_tag="IW781_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1182431.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(21404..21922)
                     /locus_tag="IW781_001429"
     CDS             complement(21404..21922)
                     /locus_tag="IW781_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1182432.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(21924..22541)
                     /locus_tag="IW781_001430"
     CDS             complement(21924..22541)
                     /locus_tag="IW781_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1182433.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(22538..23392)
                     /locus_tag="IW781_001431"
     CDS             complement(22538..23392)
                     /locus_tag="IW781_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1182434.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(23389..24300)
                     /locus_tag="IW781_001432"
     CDS             complement(23389..24300)
                     /locus_tag="IW781_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1182435.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(24341..25675)
                     /locus_tag="IW781_001433"
     CDS             complement(24341..25675)
                     /locus_tag="IW781_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1182436.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            25883..26713
                     /locus_tag="IW781_001434"
     CDS             25883..26713
                     /locus_tag="IW781_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1182437.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(26957..27206)
                     /locus_tag="IW781_001435"
                     /pseudo
     CDS             complement(26957..27206)
                     /locus_tag="IW781_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27397..27591)
                     /locus_tag="IW781_001436"
     CDS             complement(27397..27591)
                     /locus_tag="IW781_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182438.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(28096..28281)
                     /locus_tag="IW781_001437"
     CDS             complement(28096..28281)
                     /locus_tag="IW781_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182439.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(29131..29472)
                     /locus_tag="IW781_001438"
     CDS             complement(29131..29472)
                     /locus_tag="IW781_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1182440.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(29472..29702)
                     /locus_tag="IW781_001439"
     CDS             complement(29472..29702)
                     /locus_tag="IW781_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1182441.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            29989..30327
                     /locus_tag="IW781_001440"
     CDS             29989..30327
                     /locus_tag="IW781_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182442.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(30532..30771)
                     /locus_tag="IW781_001441"
     CDS             complement(30532..30771)
                     /locus_tag="IW781_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1182443.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(30747..31094)
                     /locus_tag="IW781_001442"
     CDS             complement(30747..31094)
                     /locus_tag="IW781_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1182444.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         9145 a         6671 c         5373 g        10072 t
ORIGIN      
        1 tgaaaatggc tttccacatt tttgcataaa tttgacttac taccgagaaa ctggcttttg
       61 aacaccgttt atcgagaggt tgtgaaatca gcgtcctgac ctgagtatta gagaaaaaaa
      121 cggaaaaata gttcctcata ttttgccgtt tgttgagcta ataccgaggg tcagctgatt
      181 gaacaccgtt tagtggagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc
      241 caatcaaaag cagcaaggag aacctcatct ataaaaagat agaaggaagt gagacatcta
      301 ttggtctcat ttccttctat ttttactttc ctcagtcaat tgttttacat cataaaagga
      361 gaaattccac ttattttttt cgctgtttca tgacataaac aactacaccg attaacaaaa
      421 ttcctccagc taatgctagc aacgggttat taaattctcc cgtttttggg agtccttgag
      481 tgccattttt ttcactttct tttgctgtcc tatccggatg agaaggtttt ccatttggtt
      541 ttccatttgg tgattcattt cctggatttt ctctttctgg tttgtcattt cccgggggca
      601 catcggcagt ttctcgcaca gtgatcgctt tttgctgggt aacagattct gtatgagcag
      661 accatttttc cccatcaaat atttccgaac gataagtgac ttctagcgta tattctcctt
      721 ttgaaggtgc tgaaaaaata gcggtatgat tagaaaattg agcacttgac tgtgtttcaa
      781 cgatttggat cttttctggg aaataacgca tatcttcaaa gacaggcgtt ccttgatttg
      841 gtgtgaaagt tgacacaaaa actgtaaaag gcttcagttc aatgatattt ccttctgcat
      901 tctccgccac accatttgct tcttgtataa taatcggatt attttgttca tttggttttg
      961 tctgatttgg ttcttgtacg attgcatact ctggatattc cgaagacggt gaggtttctc
     1021 cgtcaaaaat agcacctaca gataatggaa gaccatccac tctttccttt tctgtttgac
     1081 gattccccgg tttaatctgc atcagtccat gaacatggat acttccattt tcatagcgcg
     1141 taacaggctg ctgaccttct ttcgaacatg tatttagaga ctcgatccaa tcttttgata
     1201 tggtatgtcc aagagtattt tctgattgtt tctctcgttc attccaatag tagttcaacg
     1261 agtggtagat ttgatcttcg ttttgattca atgggcggat ctgctctctc atctcaagat
     1321 tctctgttcg gaaagataag ataccagaag catggtagtc tgtgtttcct gctgaagagg
     1381 tataaagagc cacattatat gaaccgttat taaaacttgt actgctattg acgataatat
     1441 ctgttccgct attggaatcg attcctttcg caagattttc gaaagacaaa ctattgatca
     1501 attcatgagg gcccgttaaa gaagaaccgc ccattttgaa tccattaccg ttaccttctc
     1561 cctcaattcc tggtacccag ccgtttcgat aagcaactga gtttttgatg attacttttc
     1621 cgatcgaacc tgtttcattt ttagcaaaaa ggtcccaacc gtcatctgca ttatgatacg
     1681 cgatacaacc gtcaaataca tttccttctc ctattgtcag tttggccgca aatccatcgg
     1741 catcttcaaa tcctggatcg gcattttcaa aagatgtaca gttcttgact aagttatgcg
     1801 cgggccatgc ttcgaaagga tcatttgagc taccgctgat ttggattcct gtatttccat
     1861 tccgataagc atgaatctct tcgatcaaat tatgatttcc agaaatctgt aatccttttt
     1921 gcatagctgc actatttgta atgtcaaacc ctcgtaaacc ccagtagtga ctgaacagtg
     1981 tcatcccatt tccagttcct ttaaaatcaa gtacaggacg cttgttttct ggatttttct
     2041 ctgcaatcaa ctgaatcggg ttttctcttg tcccgttaac gccttttgga atcgtcaatc
     2101 ctctttcgaa ggaataagtt ccaggcgcca tgacgattgt ctgcccagca tatgcgtatc
     2161 tcaatgcttg tgtcagagat aatggatttt gtctgcttcc gtttcctgca tctgaccctt
     2221 ctggtgttac ataaactgtc gaatatttag gtttacggta gtccactgta tgaagaatct
     2281 cttttgtttc ataactagtc atctctacgt attcttctgg cggatagttt ttatctggcg
     2341 taaaactgaa agtaaattcg ttcgttcctt ccatcagttt tgtctgataa tcaaattccc
     2401 gattcgcagt gatagccaag ttttttgcgt tcttaagcac ctctcctgtt gctttatttt
     2461 taattgtcaa taatccgtca gcatttgctc taaaggacat agtatgatct tggtttcctg
     2521 aatgattact agaggtcaca gtgtaaatcg gatcaatcaa gcgagtaggt ctttcttgcc
     2581 caggaggatc tgtcgcagga tctgagtaag caacttcgat tccttctacc ttgatcgtca
     2641 tattccttgc tgcagcaaat cctgcataaa taaccgattc atctgaagcg tataattttt
     2701 cccagtcata catgatgtca gaagccattt cttctcctgt ttcaggatcg taataatatg
     2761 cctctaaacc agtattcgtt tttttcatct ctacgtccag tttagtaatc gagttcgtgg
     2821 ccggaaacag attcccatga ccgtttccga atatattata agaacctgtt tctttttcca
     2881 agaaccctgc tgaagtctct aaaggtgttg tttccactga gacactccca ggagctggcg
     2941 cagcttgcgg atcttcagat gatattcctg tgattgcccg tgtccccaat ccaagtcgca
     3001 tcgtatattt gctgccgctc gtgttgacca cttcttgtct atccgagtcc cagacatatt
     3061 cgatccgact gcctagtatg gcaaatgaat ttgaataaaa tgcctgccca aacaaatgat
     3121 tttttggtac tgcgtctctt accattaaag caaatccttc ttgggcattc gtataggtcc
     3181 aagattctat ttcgatcgtc gcccgcatcc taaagttgat gtctttgggg attgcttgat
     3241 aataataggt cattccatct gatccgcttg actggaattt tccgccgtta ttcgtggaag
     3301 agagccgcac gccgtcttct aatggttcaa tggcatacct actagcactt gtgctttctc
     3361 cgaaaacaac ccctttccac tcttcatttg cttcttcaga ttgctttttt gtcttttcat
     3421 tttctgattc tttcatcata ggatcatttc cttcttccgt atccatatct tcactagttc
     3481 ttggtatttc ctccattgtt gacatccctt caatgttcac gtgtgcattt tcttcgttca
     3541 cagactgttg ttcgttagca gcaacttcaa cgacagaaat taaaaccggg gtactgagca
     3601 taaggagatt catcattagc gcactgtatt ttatacgttt ttttaacaag tgtattcctc
     3661 ccgtaacaaa ttagaaagcg tttacttaaa gtattaccta atttattttt actgtcaata
     3721 aaaaacaaaa ttataataag tgtattttat aaaaagaaaa aatataactt aagttgttga
     3781 taataaagag atttattaac ggaaaaagaa taagtttttt taaaataacg tataaaaaag
     3841 agcataaaac aaagtcgttt gttttatgct cttcttttat tattttgtgc tttttatttt
     3901 atcaaatcac tttgtttttt tataaaattt ctgcgcctag tgtggactca aaatgtctca
     3961 atgcccattc atgtccaacg ggatcgaaac ttgccacgga atctttgaaa atatggagtt
     4021 tgtatcctag attatacgca tcgactgctg tatgcaacac acagatatct gtacatacgc
     4081 cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga tctgttccac
     4141 taaaagcgga ataatgccgt ttgtctatcc aataaacatt ttcctcttgc tcatagtctt
     4201 ggtataaact ttttaaagag ccgtacaatt cccgtccacc cgttcctatc acattatgcg
     4261 gaggaaataa gcggttctcc ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa
     4321 aaacgacaaa atccttttga tcaataaact tctttgtatg agcagtcagc tttttttcaa
     4381 ttacttgtcc tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt
     4441 agtcaatcga gatcaatgcg ttcatcttag tcctccttat acgagatgtt tgacaaattc
     4501 gatcaccatt tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt
     4561 cgccgagtga tctgctgtgt cgctcattgc acgtacgatc aaaaatggga tattgaactg
     4621 acgagctgtt tgtccaacag cggctccttc catttcgcaa gctaatgctt cagggaaatt
     4681 cgttaagatt tctttgattt tatctggcga gtcgacaaat gtatcacctg taacgatcaa
     4741 tccttctttt gcgttcaaat tcgttgcttt tgctgcttta accatttcac tgcgcaaata
     4801 tgtgcttgct tcataatata gaggcattcc tggtaattgt cctgggttgt agccaaatcc
     4861 tgttgcatct gcatcaaaat aagctacttt gtctgatata acgatgtcac ctacttgtaa
     4921 gccttcaccg attccgccag ctgaacctgt attgatcacc atgttcacgc cgtattgttg
     4981 gatcaacaga cttgtggtaa tagatgctaa gacttttcct ataccggaac gtacgacgat
     5041 cacttcgtgg ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt cccatgataa
     5101 tggttcgctt aggttctctc tcaaaatttt tacttcttct tccattgcgc cgataatacc
     5161 gattttcatc tttttaattc cttctttcta tataaatgcg attgctaaaa aaaccacaca
     5221 taacattagg ataacaataa taagagattt catcaaaaaa gagttccatt tggtcgtctt
     5281 ttcccgttcg ctgatccttg ttttcgtgat tggtttattc tttttgcttt ctttacgata
     5341 aaagctggca atcttttttt cttcttgctg ataagctgtt tctgcttttc gctgtttttt
     5401 taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg
     5461 tcctttggcc ataagccgat ccctctactt tcctttggtc aatagttccc agtattgaat
     5521 cgcataaatc gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc
     5581 aagagttaga tgatacaact ccagtacttc gtcttcatct tgtgctaaag gattctcgac
     5641 cttctctacc ccttgtgcat gataaatatg aaggacttca tttgcaaaac ctggtgaaag
     5701 atacatcgaa gtaagatggc tcagcgactt cgcacgatag ccagtttctt cttctaattc
     5761 tctagctgct gtcatttcgg gattttgtcc ttctcctgga tcgatttttc cagctggaat
     5821 ctccaaaatc actttttcta aaggttttcg aaattgtttg acaagaagca gacgatcttg
     5881 ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat accagttctc tcttcgaagt
     5941 tcctccatct ggcaaacgaa cttcgtctac agcaacatca ataatttttc cgctatagat
     6001 ttctttgcga ctgattgttt tttcttcaaa ttgctcattg ttcagcatat cttctcctgc
     6061 tttctttgta atcataactg cttttttttc atttttcaat ttttctcaaa aactccttat
     6121 tagtttacca tagataaacc ttagattttc tctaaatata gaaaatcctt ctttagtccg
     6181 atactctttc tagtagtccg tatgctaaaa taagtatgta atcgaataac gaaaagaatt
     6241 caatactcaa cgataaatga catggagggt ttattatgat aaaacgaaat tggccgtggc
     6301 ttctagcatt ggtcttgcta tttctctttg tccgaaacaa tagtttttct tttgttctag
     6361 gacttttatt gataggtgta gttttagttt tgtggggact ctttggttcg cgcaggaaaa
     6421 agggaaaaca agaagaaatt cctgcactga ctgacgaact tgaaactcac tatgaaaaaa
     6481 gcggtatgac agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg
     6541 agatcaatca attgcaaatg aatatgcagc agacagccaa acttaaagca atcgatctcc
     6601 gccatgatcc agtcaaagca gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc
     6661 gactgcatga agcaagtcag tttctttata cgcatctgcc gaatctagtt gatttgacaa
     6721 ataagtataa tgagatcaat gaccatgaga tcaaaaacaa acagacctat gaaaaactag
     6781 aagaaagcgc gcagatcatc gaccagctgt cagctttaat cgcacaagat tatcaaaaat
     6841 tcgttgctga ggatctagat gatatcgacg tagagatatc tgttgcgaaa cagagcttaa
     6901 agcgtgataa taaatatgaa ccagaacaaa aggattaact cttttgttca taaaagaaga
     6961 ctcggaacat tcagatgatg tctggctgtt tcgagtactt tttctaaagt agagacattc
     7021 atgtaagaat cgagaacaga ttcaacaagg aggaattttt tatggatcat aaaccgactg
     7081 aaacaaaaga tgttacacca gttaacgata cacttgaaga tttattgaac aacccgtttt
     7141 caactccaac agatacgctg accacttcac aacaagcgga gattgatcag ctccaagagc
     7201 aacagacagc tgctcgttta attgacaaac taccggcgga acgccaagaa caagctaaac
     7261 agctagcagc aaaaatcgat gctaatgatg cacagtctgt catcagctac ggatcggctg
     7321 cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata
     7381 ttggtcctgt aggagattcc ttaactgaac tgatgtatcg tctccaagaa gccaatcctg
     7441 acgaactgcg tgccggagaa gggaacatct tccagcgtgt cttcgggaag gtcaaacaat
     7501 cgatctatga agtgacagca aaatatcaaa agattggtgc acaaattgac aagatttcag
     7561 tgaaattaga taaagaaaaa gacggtctgc taaaagataa tctgatgtta gaacagcttt
     7621 accagaaaaa caaagactac tttgatgcac tgaatattta tatcgctgct ggagaattga
     7681 aaatggaaga acttcaaaca acgatcattc cagaagcgat gaaaagagca gaagaaactg
     7741 gcgatcagat ggatgttcag attgccaatg attacacgca attcctagat cgtctggata
     7801 aacgtacgca tgatcttcgc ttagctagac agatcacgat ccagcaagca ccacaaatcc
     7861 gtttgatcca aaacacaaat caggccttgg ctgaaaagat ccaagcatct atcgctacag
     7921 ccattccgct gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt caaaaagatg
     7981 cagtaactgc tcaacgccaa gtctctgaaa caacaaacga cttattgaag aaaaattcag
     8041 aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata
     8101 tcgagaccct tcaaaccact caaaatgatt tgatcgaaac aatccaagag acattgcgta
     8161 tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga cacatggaag
     8221 aagatctgaa acaaaaactg ttggatttaa cccaatagat cacctttcgc ttgttactaa
     8281 aacaaacgcc caacaacagt ttattacaac tgttgatggg cgtttttctt atctttcttc
     8341 taacttctcc attggagtct atttctcatt catcttgctg tgtccatagc catagccaaa
     8401 ataaatgatc agtccaataa ccattacgat accgaacacg atccatgtca cagtttgcag
     8461 acgaagcatc aatacaatac aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg
     8521 cactttgaat tctccagctt ttggtttgcc aaacattttt ctcaacttca gtaagcctag
     8581 actaacaagc atcaaatagg cgagcgccat gatattcgtt agttccgcta atacattcaa
     8641 tgggaaaaat ccggcaaaga atacagctgc cacaccagcg gcataagtcg cttttttcgg
     8701 tgtacgatgc ttttgatcga tttctgtcat aaacttagga agtaagccat ccttgctgac
     8761 agcaaacagt aaacgagcaa gtgaatacat catcgagatc gttaccgtca gtagcgttaa
     8821 aattgcccct acagcgatca ccgcgcccac tgttccatgt cctacatacc tcattgcaaa
     8881 agccactggg tcattgactc ctagttcagt atacggaacg attcctgtca agatcagcgt
     8941 cacgatgatg taaaggacag tagcaatcag tatcgaacca atgattcctc ttggcacatc
     9001 tttttgcggg tttttgactt cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc
     9061 aaaaaacaca agagctgcac catctagcac accggaaaaa ccaaaaggca tgaatggctg
     9121 ccagttatca ggtttaacga aaaatattcc tacaatgata aacaacgcga tgataccaaa
     9181 tttgacatat accatggcat tattcaaacg taatgctttc ttggcttcaa gcgacacgat
     9241 gtaagtgacg aatatcacta cgagcaccgc aatcaagtca atgtatgtac cattttccgg
     9301 attgtacccg ccagttaatg cttgtggaag ttcggcccct aaacttttca aaaaaccttg
     9361 catatagcca gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa
     9421 caaccaccct gtcagccagg cagcaaattc accaaataca acatacagat aggcatatgg
     9481 cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac
     9541 aacgattgca gctaagacaa atgaaagtga cagtgctgga ccagcatttt gattggccgc
     9601 aacccctgtc accacaaata ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa
     9661 atcagacgtt tttagttctt ttttcatctc actcgtatga ttgagattga tctctttctt
     9721 acgaaacaaa tccattgaag ttcctcccca ttttaggttg gaaaacagcg attaaactgt
     9781 tttcatatac cctaaaagga taccacctaa aatgactaaa aggcaaccaa caataacata
     9841 aatgagctct tttttcgttt ttctttcgcc aaggatgaaa atcccaccta aagtagagat
     9901 aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt tgactgttag
     9961 aagcatgcag atgtttccta agccccataa taatccacag atcatgtttt tccacacaaa
    10021 ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg cctaagatca ttccgatact
    10081 ttgaggtaag ataattgaca tcgcatccaa attcgcccag ttgacgatga tggtatatac
    10141 accataacca atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat caagcatttt
    10201 attatctgta tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac
    10261 aagaagagct aaagcaataa taccgtatac ataatctttc gtttgtgtcc attcatggaa
    10321 gaaaacggct cccgcaatcg tattcaaaat taattgaaaa ccagtagaca atggtagccc
    10381 tacagaaaca cccatgtact tcattccatg gaactgtcca ttttgtccaa cactccaagc
    10441 taagccggat aaaaatccga tcaagaaaat ccaaggggtg attacaggac taacaacgaa
    10501 aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt gttgattggc
    10561 atcgccacca attttaccac ttactaaacc aatactaccc catgccaaca tagggactaa
    10621 tgctattaaa atttccataa ccaatctcct tttatttcac atgataagaa acttatcagc
    10681 gaaaacgtta acaacacaag cacttttttt cgtttctaac gattttctaa cgtttttcat
    10741 tataaaaatt taagattggg aaaaataaag tcattgttgt cccttttcct tcagttgatt
    10801 cagccgtcaa atggatacct aatttttcgg ccagattttt tgctaaataa agtcctaatc
    10861 ctgttgaatg tgttttgcta agtcgaccat ttctcccagt aaatccctta tcaaagatcc
    10921 gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt aactgaaagg ctgactcctt
    10981 gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc cggcgtatat ttgatggcat
    11041 tactaagcaa ttggttgaag ataaaagcga tccatttagc atcggtcaaa acagtatgat
    11101 cctcaccaac gatctcgtag cgaatatttt tttggataaa ataatttgcc tgactgcgta
    11161 tcacaggctg aatgattcct tttagtccat attcttggat gaggtagtcc cgcgagaaac
    11221 tgtcgagtct tgcataatac aaaacttgtt ctacgtattc atcgattttt gataattcat
    11281 tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga actagcaatt
    11341 tagaagcgga aagtggcact ttgatttcat ggacccatga gtcaatataa tctttttggt
    11401 cttgttgatt actgattgct gattgcatta cttcttggtg ttcacggatc agctgattga
    11461 tatattcttc taccagtttt tcttcttctg tcctcccacc actcaaataa ttttgcaaag
    11521 gggaagtttt ttgtatgtcg gctaattttc cccaccatct tctcttgctc aagtaaccac
    11581 ccagcagata aaaaatcaga aagacgccct caatcaaagc taaataagca attgtgctca
    11641 aatcaacttt gatgtttggc gccagccaca tcacaaagat cgtcaatgca ataaaaaaca
    11701 gccatcccaa gatcaatgtc cactgatctt tcaaatattt aaaaaaattc atcttaaccg
    11761 ctcatccttt cagggaacga tatatccctg tcctactttt gtttcgatat atccttggat
    11821 acctgcctgt tcgatttttc ggcgtaaccg attgatgttg actgtcaatg tattgtcatc
    11881 tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt tcccgactta agatctttcc
    11941 attgtgcttc atcaaaataa ataatagacg atattcattt ttgctcaaat caatgatctc
    12001 tccgttgatt tccatacttc cgttgtcaac attcaacgta atgccattgt ggctcatcat
    12061 ttcactagat aattctgtgt agttgtaaga acggcgaagt aatgcattga tttttgcaat
    12121 cagtacatcg atttggaaat gttttgtcac aaaatcatct gcccccatgt tcatagccat
    12181 gatcatatcc atattcgtat tcctgctgga gataaaaatg atcggtactt tcgatacttc
    12241 acgtattttt tgacaccaat aatagccatc caaaacggga agattaatat ctagtaatac
    12301 aagctgtggt tcttctcttt caaagtctga gaacacttga ttgaaatcgg tcgttccaaa
    12361 ggtttcaaat tgccatttct caagttcctc catgatcaat tgtcgaatga tctcttcgtc
    12421 ttcgactacc atgatttttg ccataaaaaa tccctccatc tttcttcttg ctactacaat
    12481 atcaaagaag cccttgtgaa acaagttggc ttacctggaa aatgtcattt tagcaaacgt
    12541 taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct cttccaagca gcttaaattt
    12601 attatgttat tcttaataat tggtctatta tctgttattt ttctttcgtt gtttttgtct
    12661 tttaaatttt cgataacacc aataaccgaa aagcgttagc actagcaata tacctagaag
    12721 gagtataagt aatcgataaa gatggtattt ttttgtctgt tctatctttg aagagacttc
    12781 ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg
    12841 tgtacaagtg ataagtgtga ctagatcctg cttttctcta attgctaatg tatttgtttt
    12901 tgttggaagg acaacaattt tctcaacgac ttcataagcg agcatccgac catacacttc
    12961 aagatagaat cggtcatctt gctccatttc ttcaagatcc gtaaagagtt ttttctcagc
    13021 aataccgcta tgggcggtaa tcacggaatg tgtactctct cctccagttg gaaaagaggt
    13081 gccttgcagg actgtcgctc ctctatctaa caaaagatca ttcgtttcat caaataaagg
    13141 caagctaaca ttgattttcg gaatatagat ggctccgatc atatgttgtt cgtaatattc
    13201 ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat
    13261 tcctgggatt actgatgtat gagcaagcgt ctgatttttc ttttcatgca ctgctaattg
    13321 ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt tgataattct cgatccttct
    13381 ttgatccaaa taattgctga gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc
    13441 ggcaagaaac atcactgcca taaataattg taataaaaga ctgatttggg tttgaggtct
    13501 attacctatt ttctttttca ttttcttcac cttcttattt aaaaaaactg cagcaacctt
    13561 tataaaaccg attgctgcag cttatttttg ttgcttatac ttcagacttc attttagatt
    13621 tacggtacca gctgtacgct ccaatcatca aacttattcc gattagtaag aatgccaaga
    13681 gcccgttccc accagtagca ggaagtgacc cgcgtttcat attcggtact ctttctggcc
    13741 ctggattttc tactcctacg atctgaattt cttcattata gcttccataa gagatgataa
    13801 attcagtaaa ggctgcctca cctgttggaa gtacataacc ttctggcgcc atggtctctt
    13861 tcatttgata ggttccataa tctaaccctt gaacctcaaa gtatccattt ccatctgcga
    13921 caaattctgt tgcttgtccg cttgttgccc actcaattct atctgctcct gattcataaa
    13981 cagctaaagg cgactcccct ttataaaata ttgcgtattc agaacggtct tcattccaca
    14041 attcgaatct tgcatcttta agcaattcac tgcttgatgc atcatatttt tcaaactttc
    14101 ttccacctgt tgtgactttc tctggcggct ctactccttc ttcataatca aattcatcat
    14161 ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc atccgcttct agttctgtgg
    14221 aaaccttcaa attatacgtg atcgttaaat ctcctccacg tgcatctgct aatctaccta
    14281 aagtatctac tttggctact ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc
    14341 ttactacttc tactatataa tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt
    14401 ctatttcctg atcataataa gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt
    14461 tgataacagc gcttccgttt tcaaggacag aatcgatgaa ataaggaata ggtgctgtga
    14521 tcgtgtaaga agccgttttt cctctttcta gatcaatata agaaatcgtt tcttgttctc
    14581 cagcttcatt aataatcgtt acttgtctaa ggtcgctttc ctctacattc atctgtttat
    14641 ctgcttctct gatttcgttt ttcggataca aatgcacatc tgtattgtat gtgtctttcc
    14701 atgtagaacc atcccacacg ccatccacca tatcaggcat catcactggc atcataacaa
    14761 ccattggaga cgcgattctg gaaatatgag ctggcgaata agtttctaag aaaagataga
    14821 ctttatctct agaagaaggt gcttcttccg tcacttggac ggtagagaat accgcttctc
    14881 cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt
    14941 ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga attaagaccg tcttctcgtt
    15001 ccggtgttaa aggattatct cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat
    15061 caaaatcgac tcctggaata ttttcttcac caaaatccgt agaaaccaat ccgctattcg
    15121 gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg
    15181 gtttttgtgc gacttcttcg gctttgacta gttttccgtt cccgaaaatc cctattcccg
    15241 cagtaacgag taacagtaca ctcattcccg tgacaatcat ttttttattc atttttttct
    15301 ccccgttctt tttttattaa taaaacagca cctaacaata aaagaattcc tagtaaagaa
    15361 atcaacgttc ttgcttcatt cgtttgcggg aatacggacc attttcctga ttgacctgga
    15421 ttgcttggac cactcggctc tttcggtttg tccgttgttt cttttacggt ctcgttataa
    15481 acacgtggtg ttgcggattc ataggcagag actggaactt tcccggattc taattcagcc
    15541 atctcctgtc cgttaactac tacatattgt ggttggcctt ttgcatccac ccaaaattca
    15601 gggatgatga cctcgattct cttacttgct tcatccatct cgtaaccttc cacacctttt
    15661 aattcttcaa aataataggt gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc
    15721 tctttatccg aaatgaattt gcttacattt ttgtcattca aaggatcatt gtctgctggt
    15781 ttgatccatt gatttttcaa gtcattagca ggagccatat ctaaataata tttcccccct
    15841 tctatcatct gatatagagc aaaaacagca ccttctaatg gttttccttt ttcagttgtt
    15901 cctctttgac gtccatactt gaagaagtat ggatctctta gataaccgac atttttagga
    15961 tagatatgaa tctcttgcag ttcctcggtt gattctgtcg ggtgataaat tggcaaaata
    16021 gcagcaattg ggatagctgt ctgatcgata tcaacatcca atccaacttc tggatcaatc
    16081 gattcttcga aaatcaaata aactgcatcc cgatcttggt ttttcttagg tgctttgata
    16141 cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc cagttgttac cgaagtccct
    16201 atttggacaa gcctttcttc tgtgattagc tgacggatat tgtttctgtt cgtttcagcg
    16261 atttgctgga cgaattcttt aggagtcatg ttttcagcag cttttttttc atgataataa
    16321 tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata
    16381 tctgctgttt cccctgcttc ctcagagata gccaagccat cattttcatg gtattcaaat
    16441 tgatcgttat aatcgatatc tcgaatcatc cgtttatgca acacgaattc cacgacatct
    16501 tcccctgatt cttctgccga catcgcatgt ggaagaagga ctgctgcaag cggtagtaaa
    16561 acaagtaatt gaagcatcaa acacaacagc tttttcatta ccttgctccc tcctttttag
    16621 attgtctgac aaacaaacca taggctgctc ctgcaagcgc catgaagaat gcagcaagca
    16681 ggatatagaa taatcgtccg gatcctcctg tagcaggcaa cggattttca ggatcgttcc
    16741 cgacactaag ctgtaatctg attcgatttg tctccaaaag ttcatattct aactcttgct
    16801 gtagatcagg cgtctctgct tcttcgtttg gataatacga aatctcaact tttagtgtac
    16861 ccgcttcttt tcctggaact acccgatcat tttctatgtc aggttcttct cgataactaa
    16921 tttctaaaat aaagtaacct ggaagttttc tatagctgtc aggtgacttc acttccttga
    16981 tccgatagac cccttccaac aagtctgaag cattgaatag ggaagaatcg tttggatctg
    17041 gtgccaagcc tccctcgatg atttcatact ctcctgcact attttgtttc tcaattgaaa
    17101 attctgcgcc ttcaagacgt cggctattca tactgtcaat tttcaaaagt tccaaatcga
    17161 ttggcgctag ttcatcttct aaggttactt gtagtacacc atcgatgatt tcatagcctt
    17221 ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc aatgataaac ttaccttctt
    17281 cattcagtgc aaactggaac acttgatcag atagccgata cccatttggt gcgacagatt
    17341 ccgagacctc atattcacca atagacagat gcagaggttg gtcgtaattt aaatcgacat
    17401 acttttctac tcctacggca cgagaataga agtatccgat cccaggcaaa cgtttcgatt
    17461 cttctggttc taacggatta ccagtgtatg ctcgatacag cgggaaggaa gaatccccca
    17521 ctttcctgat atcgaaacct gcaccatcta atgtatggcc ttcatggtct aattttctaa
    17581 ctcggatctc ccgataatca aacgtattgt aagcagtgaa tgctacccat aaacgatcct
    17641 cttcatctgt tgtatacgtg agatattgcc aattttcttc acctgtttgc ttgagttgga
    17701 tagtccctat agcattatcg taaagtcgca agatccatgt gtcattttca tcaggttctt
    17761 tgaaaccttc tgggttctgg ctctcaatga ttttgtattc tgcatagttg gtagtttctt
    17821 cactatcctt ataaacaaat gaatctggct ctccgattgg atgaataact ttctcaccat
    17881 ccacgattac atatttcccg tcttcatcta ctgaatagaa cgtgagtctc cctttctctt
    17941 cctctacttc gttcacacca gaaagccctt tcgctactgg atatccgatt tccagtccgt
    18001 taccatctgt ttcataaagt tcaaactctg cgccattgat tggcatatta ctgccttcaa
    18061 ttagtttatt gatattcatg tggattggct tgaattcatt tcgcatttcc agtttgacat
    18121 acaactcatc atactctgtg ccttctatgt taggctcttg tacaacagaa agcttgtgat
    18181 attctccttc ataataggaa ttcggatctt taccttccgg tccgccgact ctcgaaccag
    18241 atgtatattc atctacaagc tcgtagtgac ctaacatctc atcttcgttg gctgcatatt
    18301 ttttgatttg gaattgatac gtctcatcta ctaactgata acgtggtggt ccagcgatct
    18361 ctctgatcca aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg
    18421 tttcaaagat cgctattccg tccgtggtag ttttacttgt ttcgattggt tcggcttccc
    18481 cactctctgg attttggtca tataatgcaa attcgactcc agccaacggc tcgccatttt
    18541 gacccttctt gctgacttgg aaattacggc ctttatgttc gtaacgaatg gaagaagatg
    18601 ggaaatacat ccgtccagta gtgatatcat tctcataatc ctccaggttc tgaatatagg
    18661 ccatattgtt cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt tttacttggt
    18721 acttgatcct gaccatttcc ccttcaccta aagaaatcgg agaaaattta taagcttggt
    18781 tttcccgtcc gacatacgtt gtcggagtcg ccactacttc tggtggggct tgtccatgac
    18841 gttcttcatc ccatatttct ggctcttggt acttcatgcc gttttctcct ggatctagat
    18901 ggtaaaactg aatatctgcc gatctgccag ctccaaatgg tgagacatta aaaccagttt
    18961 catcataaag tgtggcttgc tggatcgttc tcttgaaggt agaagagata tcctccaagg
    19021 ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt
    19081 ctaaatcagc aatatttttc aatacatttc gtccaatagc atctagtcgt tgtctcccgc
    19141 taacgtttcc atcgattcct aaaccaatgg agaagatact tggcgggttc tcttgatact
    19201 tgtttttcag ccaatgatga taagcaactg tgttgatcgc agatgacttt tcttgcgaag
    19261 taggactgcc attcgtattg ttattagaag aaccatcacc aacccagtga gtcgctttat
    19321 tatctccttc ttttacctct ccataacgat agtgtacatt tacttcatct tctggacggt
    19381 taaagtcaga tgcataggta actggatgag taaaccctgc attagaagta ttcccagaaa
    19441 actcctcaaa atttctgaac aaaacgtcat tatcttcagc aatcttatct tccatcacag
    19501 tccagttgct ccaagctcca ttagtgctcg tacgataact tgactgaatc ggtgcataac
    19561 tgaaggtcgg tgtcccatct cccacaacga ttaggatttt ttctggattt ctattttctc
    19621 cgccattgtc tttatacaat gtttcatagc catttttaag ccctagtgta agcggagttc
    19681 ctccgattgg tgtacgattt aatgctgtat tctcacttcc cgtcaaacga ttgatatcat
    19741 ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt attgatagct gtactattat
    19801 ggtaaaaatt caccataccg attcgaatat ccagactcgg gtcagagagc agatctttac
    19861 tcatttcgtt tactgcctca atcaacgcag catttttagt ttgactattg gtcccagcgg
    19921 ttaattcact catactcgct gacttatcta gaacaaaaac gatatcgatt ggactgattt
    19981 cctttttctc gcccccgatg acatctagat agacattgta aagggttgtt gcatcttcag
    20041 gatgtagatc agatgctgtg ctgcttggca gaacaggttc tacccatttt ttgatgtacg
    20101 ccccgttata atccaagtaa ctgttttcaa aattccgttc atttccccaa agctgaacgg
    20161 tttgagctgg aacgtttcta tcttcttcgc ctgcagttga tccattatta gtagcatagt
    20221 cgtaatttcg ccagttcgct cccccgtttg tataatcact atcatcgatc aagttctctc
    20281 cgtcatatgg cgagcctttg tctccttgat agcgttcatt gctttgtgtc caatttccta
    20341 acacttttgt atagtcattc gttgtaaact tcgggtagcg atgctgttcg ttttcaactt
    20401 ctccgctagg atagatatat tcataggggt cttgcgtagc tgttggccca agacgctcca
    20461 aaataccgtt gaataactgg cgttgaatgc gctggagtgc ttgaccgctg ggtacgattg
    20521 gttcgtccag cttttccatc tcattttctt cgctacgttg aatttcagcg gaatcttctg
    20581 tatcagtttc attttcttgt aattcttggt tatctattct ttcatcaacc accacttgaa
    20641 gaggtagtta cccttcgttc acatcgggga tcttttctgt ttcaaaaatc aatgtgccgg
    20701 tttctgcttc ttttgaataa acttttccga cataccattc ttgaccatcg atcgtctcga
    20761 cttttaggtc ttctccatct tcatcatagg aggatagacc tgttccacga acgtttttta
    20821 cggtaccaat cccggcggct gccgcatcga ttgctaaacg tattctacct tcattggcac
    20881 tttccttttt ttcaaaagcc agatgccatt caagggtttc ttctttttca atatacttcc
    20941 cttcaatttt tgcatagtcc tcatctaaga atacctcatc ctcctttgtt tctgttgact
    21001 cagcagctac atccagggtc gtcatatgat agactgactg gaaatatggt ccaaatagca
    21061 aactgacagt tactaactgc aaaagcttca attttaaccc tttcacatcc ttatactcct
    21121 ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaac aatgcccatc taattataat
    21181 ttaatttaat tttaatttta aatcaaatga tatccttttt agttaaaaaa caccaattat
    21241 acaacattta ttattaaaat taaattataa aattttataa cgttctcatt taacaaaact
    21301 taagttatta agacaaatga ataaacaact acgctttcct cgaaacaaaa aaataaagca
    21361 caaatgcgca tttacgcact catgctttat tcgtacaatt aattcagata acggcagcgc
    21421 ataggatgcc aataacactt cctaaaatag ctcctcctaa aatgtcagta ggataatgta
    21481 aaagtaaaaa aatcctcgaa taagcgatca aacaagcaaa taaaaaggct aatattcctg
    21541 caactggact taattcgaac aacgcagcta ctgataaaaa ggctagcgct gtatgtcctg
    21601 aaggaaaaga agaagtcttc ggtttacttg tgtaaaggac aagccgacga tttttttcat
    21661 aaggtctttt ccgtcttgtc gtttttttga taaaaagcaa accaacaaaa aaatagatca
    21721 aactgccgat aagaatggct agaccatatt ctctagtatc aggtatcaag actaaaacag
    21781 cagcaaggag caaccaaata aaaccaccat tagagatatt actgataaat ttcaaaaaat
    21841 tgatttttcg actatccgtt ttccgttgct tggcgaactt gttgataata ctatcatcga
    21901 ttttagcaat caaacgcaac atgttattcc tcctttttaa gaaccttttc ttgcgtacga
    21961 tggataaaaa gcaggacttg aaaataaaac aagaaactga aacctaataa agcaaaatag
    22021 attggtcctg caaaccagaa tgcgacaaga aggatcaacg tgcctgccaa atagaaaagc
    22081 gcgcttttga aatcattaaa aaacaagaaa aatgcagtaa tccaagaagc ttctttttgt
    22141 tccagttgaa gcaagatttt gtaactaaag agaagcgcgc ttaatagaag caaaaacaac
    22201 gtagtataca tcaatgtata gccaattccc ccaccccaat agcggaaaat ctgataatcg
    22261 atcaatagaa gaagaaaata aacacttgct aaactatctt gctttccata aagagagaag
    22321 ttctcacgaa actttttcca gaatagtgaa ccgagctttt tctcttcatt ttttttgtaa
    22381 tgataaatca tatacagaac tgttcgactt gcagttccta atgtgaaaac aggcaagacg
    22441 cacaggaacc atagtagctg taaatggata agacgtggaa tccattcaat gatttctaag
    22501 aaataatcat tggctttttt accaatcgat cgattcatca tccttttgaa cctcctgtca
    22561 aatccatccc ttcaatgaaa taacgttgtg caaaaaagaa cagcaaaatc gtcgggatca
    22621 agacaagtgt tgctcctgcc atcagataat tccattgtgt cgttgcttgg ttctgaaaaa
    22681 cttgcaagcc aatctgtaac gtgtattttt cttgatcttg gatatataac agaggaccta
    22741 agaaatcatt ccaagctgcg ttgaaggaat tgatcgcaat ggtaatcaag gcaggtttcg
    22801 tcaatggaag catcaaatga ctccaaatat acaaatgatt cgctccgtcg atttcagcag
    22861 cttcgatcaa ttcattattg atcgataagt aaaattgacg catcagaaat atattgaacg
    22921 cattgccgaa gaacgatgga acgatcaggg gcaaataagt acctacccag ccgatcttgg
    22981 agaataatac atattgaggg atcatcgtta caaaccccgg gatcatcatc gtagaaagaa
    23041 ccaaagcaaa catcagcttt ttcccaggaa aatcaagctt cgcaaatcca taagcaataa
    23101 aagaatttga aaggacatta ccgatcacta ctagcacagt gatgaataat gtatttcttg
    23161 catagcgcca aaaaggaaag gcggcaatcg tatccagata gttttcccat acgatttttt
    23221 ctgggaaaaa ggtcggagga taacgaacga tttcttccat tggtttcaat gaagtaaaca
    23281 ccatccaaag caatggaatg atcaaaatga ttcctattac tgtcatgagg acataactta
    23341 gtattttttt tgtttgccga ttcacactgg tctttttgaa ataatccatc atttgccgtc
    23401 acctccagaa taatagaccc acttaggtgc aagcttcaaa ttgacaaacg tcagaaccaa
    23461 cacgatcaga aacaggatca ttgacatagc catcgcataa cccatatcaa aagctacgaa
    23521 cgccttgttc cacatatgca gattataaaa aagcaaagaa tttgcaggtc cgcctgttcc
    23581 atttttcgtc atgacataag cttcttgaaa aatttggaaa gaaccgatcg tagaggtgat
    23641 cacatcgtaa aaaataatag gagtgattaa tggcaaagta atacttttga actttcccca
    23701 aacgcctgct ccatcgattt ctgctgattc atataaagat ctaggaacat tttgtaatgt
    23761 agctaagtaa agtagcattg ctcctccaga actccatatt ttcatcaaaa tcaacgcagg
    23821 tttcgtccat tgtggatcaa acagccaagc tggtccaggg atatggaacc agcccagaaa
    23881 tgtgttgatc aatcctgcag atggagaaag cagctgcatc cataagatgt agactgctac
    23941 tcctgataag actgccggta aatagaaagc tgtacggaat acactgattc ctttgatttt
    24001 ttgattgagt actgtggcta aaaacacact gaaaatcgtc gtcagcggta cattgaagat
    24061 gacgaaataa acggtgttgt ataacgattt ccaaaataaa tcatcgtgaa cgaacattct
    24121 tttgaaattt gctagaccaa tgaaattcat cttggatgtc aaattataat cggtgaaact
    24181 accaataact gaaaaaagca taggccctag cataaatacc aagaacccga taataaaagg
    24241 cgagataaat aaataggccc atcttgcttc ttttttcttt cggatagata aacgcttcaa
    24301 attttcttgc ctcctctgta cctagacagt caaaaatgga ttattgattt tgcttgacta
    24361 agttttctac tgctttttgt gcatctgcca atccttcttt tggtgatttt gttcctaaaa
    24421 gaatctggtc aatctgttca ttcactaatg tcgtgtaatc cggagcatct aaaggaactg
    24481 gtgtgatcac cgtattttta aagttttcgt ccgccatttg atagatcatt tttcctttat
    24541 ccgttaggtt gtcattattc actaaattct cattggctgt cttgctcgcc atgatatcaa
    24601 aacttttttc gccgaatttt tcttgtactt ctggagaaga aagatatttg atgaattcat
    24661 aagaggcctc aggatctttt gctccgtatg gtacttccaa gacgaatccg ccaccccacg
    24721 accaatggcc tgaaccttct tctttttctg ggagctgcat cacgccaaaa tcaaaatcat
    24781 ccggtgcatt ttccatcaaa ctagagtaat aattgatatt ttgggcacgc atggctacta
    24841 atccggaaat aaatggatca gatacaccag acccaaattc ggcttctaat ttattgatcg
    24901 tatttttccc ataataatct tgccagtctt ggatccactc caaggcttcc actttatttt
    24961 tagtatcaat cttgactttt tcatctttgt caaaccaact agtcccgtta tctgcgttta
    25021 gcgcccatac atcagcccct aaattccata atggataaaa accgatgcgt gtaaattctt
    25081 ttccttcttt tttatctagt ttacgtgctg tttcttctaa ttcatcccaa gttgtcggta
    25141 gatcttgttc actgattcca gcttctttaa ataacttttt attataaaaa atgacttgtg
    25201 tatctgtatt gaatgggatc gcataaggat caccttcata ttctactgta tcccaaagct
    25261 gcggataaaa atccttagaa gtgtcttttt ctacatattt ggagagatta gttgcttgct
    25321 gtgcttctgc tctttgggca acggaattta tgtcctgtac aataacatcg ggtgcatttc
    25381 cagctgttat tgcagataaa gatttagtcc agatgtcacc ccatggctga tacttataat
    25441 ttacatggat ctcgttttgt gattgattga aatcctcaat gatttcttcg atagcttctt
    25501 ttcgtgcacc ggatccccaa aaggaccaga aatcaatatt ttttcttcca tcacttgttt
    25561 tttcagaaga atttgctgca cttgaactgg catcgcttgc attacttgat ccgcatcctc
    25621 ctaataaaaa caatgctgaa cttgccagca accctgtaaa caataatttt ttcatatttt
    25681 acctccaaat tttaatgtaa ccgatgccgg ttttaatata acgatatcat tttgttctga
    25741 aaataacaat ttataaggta tgattctaaa aaaattatta atgataccaa tactttatat
    25801 aattcgcgag cgctttcttt gaatttttta atctttcgta gcagaatgag aatgtattaa
    25861 aaatagatta ggaggaattt tgatgtttag acaccagaaa gaattgcagt ttgaagcaaa
    25921 accagatcgt ccgaatccat taatcgcgaa atatctgcag gagctgatcg gcggccaata
    25981 tggcgagatg agtgtagcta tgcagtacct gttccaaggc tggagctgcc gaggagaaga
    26041 aaaatataaa gatatgctga tggacattgc aacagaagaa atcggccacg tggaaatgtt
    26101 ggcgacaatg gttgctcgtt tattagaggg tgcgcctgtt gaagatcaag aagcagcgat
    26161 gcaaaaagat ccaacaatcg gtgccattat ctctggaatg aatccacagc acgcgatcgt
    26221 agctggctta ggacctcgtc ctgctgacag cgtgggcaat ccatggagcg gtgcctatat
    26281 cattgcgagt ggcaacttgt tagcagactt tagagctaat ttaaatgccg aatcacaagg
    26341 aagacttcaa gtagcacgta tttacgaaat gatcgatgat cgtggcgtaa aagatatgct
    26401 gtctgttctc cttgcaagag atagctatca tcaaaatcta tgggaatgtg cagtgaaaga
    26461 attagaagaa aaagaaggtt caatcttagt cccaagtact ttcccgcgtg acaaagaacg
    26521 aaaagacatt gcttatgact tctataactt ctcagaggga gaccaaagta gtgaaggttc
    26581 ttgggctagt ggcaaagcac tggatggcga aggcgaatat caatggttga aagaacctaa
    26641 gatcgaaggc gctgcaccaa aattagaacc agtaacacca aaaatgtatg ggacacctcc
    26701 attcaagaaa taaatatcta cccgtagaaa cacccgtttt ttgttccaca cgctttgtga
    26761 gcaaaactga ctaaagtcat taatcataac cacccttcaa aagggtggtt ttctctagag
    26821 ctataagctc aaatcaccgg ccagcgccta aagacgctgg ctttcatatt ttatgttcaa
    26881 gccatattgc ctttgccagt tttgcccacc tcttaaaagg tttatgtact actccttagc
    26941 ccttaaaagg atcgtcttac tctcgtacac tcagcttatc taacgcacta tcatgttttt
    27001 cttgttcttg aatgtacttt ttgatggtcg cttcattttg tcctactttt gtcacatagt
    27061 atccttccac ccaaaagtgt cgatacccaa atttatattt tagatttgca caatctcttg
    27121 atgatctcct gaatattggc tcgatattga ttataaatta tttttcttct atactttggt
    27181 gtgaatacaa tgtggtattt acacatctac tttgtacggg ctaaactatt tccctttatt
    27241 gctccacccg catagcaagt ggttttgtat ttcaatcgct cggcgcgctc aattaggtct
    27301 aaagacaata aatctaaaaa cccgctgctt cactgacaga aacaacgggt tttcagaaaa
    27361 aaatgaaatg aaaaagatct cttacggcaa tctattttat ttttttgatt ttttataaat
    27421 ggaaaatgct tcaagaagtc caagaaataa agccgtataa aaacaagaac ctatgaaatc
    27481 tttatttatt agatattcaa cagaaatccc gataacacta gccaacaata tatttatcag
    27541 aagtgtaaaa taaggatgct ttttatagaa tagaactagt gttgttttca tagactctcc
    27601 tttcttcata tttccaatta atcacaaatt cacggcatta ctagaaaaat taaaggggcg
    27661 cagaagtgga aaaatactct tcgactttcc ctacaaacca ggccatcttg tgtttaataa
    27721 ctataccaaa tctaaggtac ctctaaatct aaattccatt tgtggcggtg acctccaatt
    27781 tttaactctt ctatctccca tccagtatac ataaccttga tacttcgacc acttatatat
    27841 cgaacacaca aatacctatc cattgcagga gtactaccta acttagttgc tacttgcatc
    27901 taaataacag ctgaaaagat aggacttact cttagtaaag tatctgaact attaattttt
    27961 aaactatcat ttttatcctc aactacatgc acatctgttg caacttcact ttctgtctca
    28021 tccagataaa tgattaagga atcgggaatt gcaatatctt cagctgacgc tggaatagtt
    28081 gagctaaatt agtcatcata tctttattat aaaataacca acagcaaaaa atacaatgaa
    28141 aaacaaactt ggaactaatg tatcattaat aaatgttgaa aaattcagca actcattact
    28201 gtatgatttt agcagcccaa ccaacatata ccctataaaa gctaataaga tggttaggaa
    28261 tcctttagaa tttttactca ttcatagaca cttccttaaa taacttttat tatagaaggt
    28321 attgtaaata ataatattga caaaaagtga ctcaaaataa aaaaaacctt acattatact
    28381 ggaatgaact gtattgatag tgtacctaaa agctctactg acataagatc gtgtaatcgg
    28441 taaagtaatt ttattaatta tacgggatgt aatcaattag agaataaact aatcataaaa
    28501 ggtactttta cattcataat tgtcgctttg agattctact ttggttcact atctgaccca
    28561 attgcacatt ttcgatcatt acaagtattg gtgtcgttag aacaactaaa taaaaactag
    28621 cggtagattt ttcagaatct atgctaattt ctgtatggat gttccagtga tggcaaattt
    28681 ctcttttcat tgtttttctt gtcgcatgat atgccttttt gtgttattca gttaaactat
    28741 agaaattctt tcctagacag atcttgatcc caaattttac gtttttgagc atcgatatag
    28801 tttaaaatga cattactcat atctaaagag tttagatttt ttaggactaa aactgtcagt
    28861 tgttcttttt cttctttctg ttaaagtatt gtcgcgactt ttcgtatacg ttaagatatg
    28921 tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga cttgaggaaa tcacctcaat
    28981 cattcgttta aaatgctata tattctttag tttaagatac ggaaattaat gccatcgacc
    29041 acactagtag aatgaacgac atttacttgg ccttttttta atttcgttat gtttagcata
    29101 taaaaatatt atagagttta cttacaatgc ttaaaacata tactgtataa tctgatcaat
    29161 tttagccatg tcttgcaatg gtaagttttc tatttttttg agttttcgtt ccttaaaatc
    29221 aagagatttt aattgtgtaa taattacttg tccttttata tctagctctt caggtaatga
    29281 gtaacgtgtc ggtaaattct ttatagtgga agtaattgga catatgaccg aaaacattgt
    29341 tttacggtta aattcataac gagagacgac tagtcctggt cgtctttttt gaatttcttt
    29401 tcctacggag ggatcaaaat caatccatac aatatcacct tttttaggaa tataaattct
    29461 ttcttctgac actataaaat ttctctccct tcaggagtta aatcttccca ttcgtctttc
    29521 tcataatact cggcctcttc cccaccacta aatggatctt caatttttgg aactaacgta
    29581 attgttccat catcagaata cacaacgata tattcttggt tttctgaagg tttctttccg
    29641 ttatcagaag gtaaagttac cactacagaa cttccttgta atctagattt tgtaacaagc
    29701 attatcatca ctcccttaat aaaagtatac tatataacac taagtaacgc aaaattcccc
    29761 tttgtaaact atctagaaaa ttgttttgtt taaagtatat cttattggat ataaatagaa
    29821 ataaattgta gccagttcat tttttcttta actaaaaaaa taatccaaga aacattgaac
    29881 ctatggatcc aaaagctacc caaatactat agctaacccc ccgctacctt caattttgca
    29941 gataaaacgc tttgggagaa aatttcaaag acaacaggcc atcctatcat gcccgattat
    30001 aaacaaaaag ctggctattc cttttgctct gacaaatttc aattgggaaa gttcacgaaa
    30061 ctagcaggtg aagaagtttc aactgttcgg atcaaagaat gcccgcttca aatagaaaca
    30121 ttggttcaac gaatttcaac tagacaagac tttgctattg tagaacgtga gatcgtagct
    30181 atttttgtag aagaagggat tttacatgat ccaactcata tcaatgtgga taaatggcaa
    30241 cctcttatct ataaattcag agaatataca actgccaacc agtcgttaac tttgaatttt
    30301 agattccaag aattcaaaga gtcataatga taaacatcgt taggtataaa taaaaaagac
    30361 tcgcagcacg agtaaaaaac aaatagaagc tatacattag aaaggtcgac tgtgacccta
    30421 tagatgcagt atatgcagct tatgagcatt tcttctgttc tctttgtttt tgggatctgt
    30481 gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa aatcgattat gttatacctt
    30541 ctccattact aattcaggca tctgctgagt aatgattttt cgcgcaaaag aaatatctgt
    30601 tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc attcggtcat ggattggtgc
    30661 gactgtgcgg ttaggttcag tagtcattat gattgattct gccatttttt catcctgatg
    30721 gctacgataa aaaccgccaa tatacactat tttttcctta tttgaaaaca taaatttgtc
    30781 tttttcttca ttccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag
    30841 gtcttttttt cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat
    30901 ccggtaaatc cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac
    30961 gtcacaacaa gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt
    31021 tgtttcgtct gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa
    31081 taacgaccac acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa
    31141 gttcgcacca acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca
    31201 aactctccag tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac
    31261 a
//
LOCUS       DACEOM010000034        30436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEOM010000034 DACEOM010000000
VERSION     DACEOM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.035"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1237..1902)
                     /locus_tag="IW781_001443"
     CDS             complement(1237..1902)
                     /locus_tag="IW781_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1182445.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2028..4064
                     /locus_tag="IW781_001444"
     CDS             2028..4064
                     /locus_tag="IW781_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1182446.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4068..4706
                     /locus_tag="IW781_001445"
     CDS             4068..4706
                     /locus_tag="IW781_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182447.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4734..5162
                     /locus_tag="IW781_001446"
     CDS             4734..5162
                     /locus_tag="IW781_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182448.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5619..6530
                     /locus_tag="IW781_001447"
     CDS             5619..6530
                     /locus_tag="IW781_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1182449.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6747..7184
                     /locus_tag="IW781_001448"
     CDS             6747..7184
                     /locus_tag="IW781_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182450.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7279..8163
                     /locus_tag="IW781_001449"
     CDS             7279..8163
                     /locus_tag="IW781_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1182451.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8387..11554
                     /locus_tag="IW781_001450"
     CDS             8387..11554
                     /locus_tag="IW781_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182452.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11574..12119
                     /locus_tag="IW781_001451"
     CDS             11574..12119
                     /locus_tag="IW781_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1182453.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12147..12602
                     /locus_tag="IW781_001452"
     CDS             12147..12602
                     /locus_tag="IW781_001452"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1182454.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12599..13357
                     /locus_tag="IW781_001453"
     CDS             12599..13357
                     /locus_tag="IW781_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1182455.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13443..14183)
                     /locus_tag="IW781_001454"
     CDS             complement(13443..14183)
                     /locus_tag="IW781_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1182456.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14244..15497
                     /locus_tag="IW781_001455"
     CDS             14244..15497
                     /locus_tag="IW781_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1182457.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15819..16046
                     /locus_tag="IW781_001456"
     CDS             15819..16046
                     /locus_tag="IW781_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1182458.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16169..16495
                     /locus_tag="IW781_001457"
     CDS             16169..16495
                     /locus_tag="IW781_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1182459.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16563..17276
                     /locus_tag="IW781_001458"
     CDS             16563..17276
                     /locus_tag="IW781_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1182460.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17586..18437
                     /locus_tag="IW781_001459"
     CDS             17586..18437
                     /locus_tag="IW781_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1182461.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18558..20423
                     /locus_tag="IW781_001460"
     CDS             18558..20423
                     /locus_tag="IW781_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1182462.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20471..21880
                     /locus_tag="IW781_001461"
     CDS             20471..21880
                     /locus_tag="IW781_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1182463.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            21988..22689
                     /locus_tag="IW781_001462"
     CDS             21988..22689
                     /locus_tag="IW781_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1182464.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22848..23327
                     /locus_tag="IW781_001463"
                     /pseudo
     CDS             22848..23327
                     /locus_tag="IW781_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            24175..24750
                     /locus_tag="IW781_001464"
     CDS             24175..24750
                     /locus_tag="IW781_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1182465.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24750..25190
                     /locus_tag="IW781_001465"
     CDS             24750..25190
                     /locus_tag="IW781_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1182466.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            25206..25907
                     /locus_tag="IW781_001466"
     CDS             25206..25907
                     /locus_tag="IW781_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1182467.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(26395..26820)
                     /locus_tag="IW781_001467"
     CDS             complement(26395..26820)
                     /locus_tag="IW781_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182468.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            27194..28165
                     /locus_tag="IW781_001468"
     CDS             27194..28165
                     /locus_tag="IW781_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1182469.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            28197..29594
                     /locus_tag="IW781_001469"
     CDS             28197..29594
                     /locus_tag="IW781_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1182470.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            29753..30376
                     /locus_tag="IW781_001470"
     CDS             29753..30376
                     /locus_tag="IW781_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182471.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT        10332 a         4937 c         6272 g         8895 t
ORIGIN      
        1 actacctgta ttagtcctct tttttgacca gattttgacc agtctatttt acattctaat
       61 atattcatat gagctaattt tatgattaaa tggtaagtgt ttccttgttt gttttagttt
      121 aaactagtgt gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa
      181 ggctaggaac ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat
      241 aatttctttt aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca
      301 gagccgaaac agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga
      361 gttggcaaaa gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta
      421 actgtaacaa gctaatttta agagattctt attagaagac gtttaggaat ataactaaga
      481 aaatgattat acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt
      541 taagaaaaag gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat
      601 ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat
      661 aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga
      721 aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt
      781 ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata
      841 aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg
      901 agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact
      961 acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa
     1021 aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt
     1081 ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga
     1141 actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt
     1201 gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg
     1261 gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat
     1321 ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc
     1381 atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa
     1441 gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt
     1501 atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg
     1561 ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt
     1621 tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg
     1681 caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc
     1741 taaagtatga tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca
     1801 actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt
     1861 acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt
     1921 ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg
     1981 aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa
     2041 aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg
     2101 ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt
     2161 atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca
     2221 tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg
     2281 aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat
     2341 tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct
     2401 ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct
     2461 tgatgacgag agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca
     2521 atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa aatagctatt
     2581 cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta actattggat
     2641 tgattctttt attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt
     2701 tattgttgaa taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga
     2761 aaatcattat tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata
     2821 aggagcaatt tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta
     2881 tctctttgat ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact
     2941 atatccaata cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca
     3001 atgtcgaatt agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac
     3061 caaaagaaat cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc
     3121 acgtggattt tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa
     3181 ctttgaataa aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg
     3241 aacagtttgg cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg
     3301 atgtctctgg aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc
     3361 ctgaacgata tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc
     3421 aagaaatcag tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata
     3481 catttattcc ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga
     3541 aaaacagtat gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga
     3601 tcaagatagg ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac
     3661 gtgataactt gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt
     3721 taagcagatc atttcattac ttgatacaga tatttttact cactttcttt tctttcttct
     3781 tgttgagcta tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct
     3841 ttcgttcaag tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca
     3901 tcaaatgggg aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc
     3961 tttttaatat ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt
     4021 ctacagaaaa gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga
     4081 tagtgataaa aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt
     4141 tcactgcgga tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta
     4201 cgttattgaa tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg
     4261 atcaagagac gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat
     4321 cttttctttt tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata
     4381 ttggattgaa gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat
     4441 tgaaaaaagt aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg
     4501 agaagcaaag aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg
     4561 atgaaccgac tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc
     4621 aacaagcttc ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa
     4681 taggaaacaa gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa
     4741 aagcagttat tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg
     4801 aagtggaagc aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga
     4861 ttcattttga tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt
     4921 ctagaacagc aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta
     4981 cgagaaaaac aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag
     5041 cgaaagttgg aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa
     5101 gtgcgacagg aaatagaact gacacattct catgttggta caatccaacc ggttggtatt
     5161 aagtatccaa aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta
     5221 tttttggtag ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca
     5281 acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg
     5341 aagaagaaaa acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac
     5401 gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac
     5461 ttgacattta ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac
     5521 gtatcgagca gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt
     5581 agcaattcgc aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac
     5641 ttgccatcgt actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag
     5701 ccgttagtta taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa
     5761 tgcaaggagc agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat
     5821 atggagatga cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat
     5881 tgggtgattc atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag
     5941 acagtatcaa acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg
     6001 atgaagattt aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc
     6061 aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt
     6121 tctctaaatt agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag
     6181 tgaaatttga ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt
     6241 tgaaagatgg tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg
     6301 aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca
     6361 aaaaagaatt gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata
     6421 aagtaattgg cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa
     6481 atgttttgag tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa
     6541 taaaaaatat gaactattta aatcaataaa actgttcatc taaatctttt tattagaact
     6601 aaatcaacag tagtttaatt actacttgat ttagttcttt ttattttaaa aatttttttt
     6661 acttagaatc cagcgaccct agatattttt ctgtataatc ttaaataaag aaaacaatat
     6721 caaaaaatgc acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag
     6781 aagttattca cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg
     6841 atcatttgta tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta
     6901 tagcggggat tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt
     6961 ttttcagtta tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga
     7021 agaaacgcta ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta
     7081 ctttattagg tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt
     7141 tcctcagttt catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa
     7201 cagtacttca tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat
     7261 cagaagggaa agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt
     7321 gaacaagcaa aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa
     7381 gtggtcgtct tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct
     7441 acggaagggt atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc
     7501 agtggaatcg ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg
     7561 aatgacttgg ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt
     7621 tcgattgaat cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat
     7681 gtcgtcgcaa ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg
     7741 aaatttggaa aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc
     7801 tactcgtttc acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg
     7861 ataggactaa ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat
     7921 gatggtaagc tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca
     7981 ttgccagacc tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt
     8041 gagcaattag ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat
     8101 gaaggggata aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt
     8161 taaaatggag gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga
     8221 ctgtgacaag acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata
     8281 gaaaaatttt tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta
     8341 aaacgataaa ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt
     8401 aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt
     8461 tgccgatgta gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac
     8521 tactttttca gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac
     8581 taataaaagg caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc
     8641 tgatagcagt gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc
     8701 cacaacagaa gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat
     8761 tcagcctttg actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg
     8821 tggattcgct cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat
     8881 cgagttccag aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga
     8941 tggaaatgcc attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac
     9001 gggagaaata aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat
     9061 ttacgctcct cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc
     9121 agtgaaggaa ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt
     9181 gaatgtgatc aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct
     9241 agattcagga gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg
     9301 ttcaaaggtc tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt
     9361 cgatacgcta cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt
     9421 aaatacaaag atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc
     9481 aggatcgaaa gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg
     9541 cggatttcgc ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc
     9601 ggtcaaaatg gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa
     9661 aggcctttct ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg
     9721 gcgttccagc gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg
     9781 ctcattgagg gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg
     9841 aaatcaagcg atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga
     9901 ttttacagtc actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt
     9961 agatacaact ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat
    10021 ggtcgggaaa acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga
    10081 cttagaatct cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg
    10141 cataggcggc tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga
    10201 aatcgcttta tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta
    10261 tttttctgat atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag
    10321 ccctagtgca ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa
    10381 tcgaatcagt gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc
    10441 agataaaaaa atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga
    10501 tgcttggaca ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc
    10561 atctgtagaa attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata
    10621 taacccatgg ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga
    10681 gagattaaaa acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca
    10741 agaagtgaca gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc
    10801 taatttaagt accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc
    10861 aggagccttg gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga
    10921 tacaggggaa tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga
    10981 aattgtatta aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag
    11041 taccaacgat gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc
    11101 cccgcctttt gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc
    11161 ttccaaactc gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca
    11221 aatggatata caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat
    11281 cttaacgata aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt
    11341 cctatatttc aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt
    11401 cagaggacaa aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtcg
    11461 ggcgtttaaa cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat
    11521 aacatggaca ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc
    11581 gaatatggaa taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat
    11641 tcatcctttg gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat
    11701 taccggctaa tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga
    11761 aaacacagga aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga
    11821 cattgatcca ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa
    11881 gtaaatttga tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa
    11941 taactgttcc tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag
    12001 agctggctgg tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta
    12061 acaaaagtac agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa
    12121 atagcgaaaa agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg
    12181 atggaagaaa tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct
    12241 tgactacatc ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa
    12301 aagaaaaaat agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa
    12361 aaaaattagc tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct
    12421 ggaatctcca taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg
    12481 atattgattt aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag
    12541 tcattcttat tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat
    12601 gaaaaaaaca attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag
    12661 tgtttttgct gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc
    12721 agatgctgca aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga
    12781 tactcctttt acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca
    12841 agctccagat cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc
    12901 attacaaagc ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt
    12961 aacggataaa agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat
    13021 tggtacaaca tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt
    13081 gattcgaaat agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc
    13141 tgaaggaatc gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga
    13201 agggattggc acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt
    13261 ggaattattt gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg
    13321 gtcgttaaca gatgctccta cagaagagga agcgtaaaat catatcaacg atttcaggga
    13381 gtggagccga ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt
    13441 catcaactcg aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc
    13501 tttcgaccaa cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca
    13561 attttctgca attgcgataa gttgatacag taagatttta aaagtaaaaa gtaagcatac
    13621 ttttctaact ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt
    13681 gttttgacca taacttggtt tttcaaacct ttgatacttt caatatataa aatttcccga
    13741 ggatacagga cgagacttcc acctttttga tcaataaaga ttttatcgac atttacgatc
    13801 tgctgttctt tccaattgat ctgttctagc acttgacgaa tttgttcaga taaattcctg
    13861 tcttttctga tatatccaat cggaaggatt ttccgattga tgatctctac tgcaaaattc
    13921 gtataagcgg tgaaaaaaac aatattacta tgtgcatttt ttttacgtat ttgttcggcg
    13981 acatcaatac ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac atcgtgatta
    14041 tcaatagtgc ccaactggat caactgattg atttgagaga tagaagtgag ctgcacgata
    14101 ttctcgatat gatgattgac caattctctg atgatccatt cgctgtgaaa ctggttatct
    14161 tctatgataa aatacttttt catatgcagg ctcctattaa aaaaatatgt gtacattttg
    14221 aaacaaaaca atggaggaat aagatggtcg caacagtgac actttatttg acctttactt
    14281 tgattttaga tttaggtatc tatttattct tttcgaaaca actgctagta aaaaacagct
    14341 ttggttgtat agctttgatg gtggtgacac ttttacttcc tatccctaaa gtgagctttc
    14401 cttacctagt tggcttatta ttgatgtttc ttttttattt tactacgaaa aacctgttta
    14461 tcagctgttt gatcagtaat cttttttttg ctatcactgg actcagcttt tttctggttt
    14521 ttgatctgat ggattattta ggaaagagac atcttggaac agcaagtttt tttgcactac
    14581 ttatcgcatt tattttctat ctattagtaa aaagtataga caaacagacg agatttctca
    14641 acttgctttt tacttatcca actgaacact attttggagg tatttgtatt ttattaagct
    14701 ggggcgtgat ttacttggta ttgggaagtg ttcacttccg ctcatctaat tatctgcttc
    14761 tatctctttt gtcgatagtc atcagttttt tttctttgtt tttttccatt atgttgatca
    14821 gtttccaagt aaaagaaaga caacatcagg aatcgcttca actgtatcaa ataaatgaag
    14881 aatattacca tcatctagag gaattcaaac atgattataa aagtctactg ttttcattaa
    14941 aaattactct tcaggaaaat aaaaaagaaa cgttggaatt tttagaaata ttagaagatc
    15001 attctgcagc tatttttgaa aaaccgcaaa tacaacagct aacaaatatt gtatccccag
    15061 ctgtcagggg tgtcttttta aaatttctag aaaaagcaga atcggagaaa atcccgatcc
    15121 aagtagttat tcctgtcgaa gtgaaaacaa tcccgattga tctggttgtt tttattcgct
    15181 gtttatctat ttttatttct aacgcattcg atcatcgtgt agccgatcag agtccgatta
    15241 cgatcaatct gctggaagaa aaagagggat cacgttttag tatagcgaac aaggcaaatc
    15301 caacgaattt gacgctatcg gagatggtca aaagaggaaa aacctctaaa aaaaatggcg
    15361 ggttaggtct atacagtgcg aaaaaaatgt tggatgttta cgaaaatgca gaactaaaaa
    15421 tcgaattttc aaaacagaat cactattttt ttgtagaaat gtatctggca tctagaaaga
    15481 gcaaatcagc ctactgactt actgtcaaag gataagtttg aggaaagtct acacggagat
    15541 agagacaaca acacagcata ttcaaaagaa acagaagcgt gatgaccgtt gtcacccttc
    15601 tgtttctttt gctatgtagt taaacaaagt agttgacact tccgctactt agtattagta
    15661 cgaaagtata cttcatgata ctaagtagat aagaagtgaa aagcggaacc cacgatcgtc
    15721 aaaatgctaa ctgctttgct tacaccagat aaagggaaga gcaatatcgc ttattttagg
    15781 cgtggagaag caagcaaatc cctacgatca caaaaaaaat gctgacccag cagttaagag
    15841 agttagaaca agatgaaagt atccatcgaa aagcctataa ccaagtcccg cctaaagtag
    15901 agtactcgct caccgaagaa ggaaaatcat taagagagat tctagtagcg ttgtctgttt
    15961 gggaagaaga acgtatcgaa aaacaaaaag cctcaaggga agaagacacg ctactgcata
    16021 cacatggtgg ctacctaaat tattgatttt ctatgacatt tattttaaat aaaagaaaac
    16081 aggagttttt ttaaaagcat catctatttt ttatgtaaaa aaatggtatg attaatcaag
    16141 taagaagtga ttggaagaag gcaagcaaat gaatagtatg gaaaaagtca accagtttag
    16201 agatgaacgt aattggcgac aatttcataa cgaaaaagat ttggctatct ctatttcttt
    16261 ggaagcatct gaattgttag aactttttca atggaaaacg ccggaagaag taaaagaaac
    16321 acaattagaa cgaatcaaag aagaactggc agatgtattg atctattcat acatgatggc
    16381 agacaacctt cacctggatc tagatgagat cattgaagaa aaattgatca aaaataattt
    16441 gaaatatcct gtagaaaaaa gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg
    16501 tgtcattcgg ctgttggaaa gagcaaaaga aaaacagtaa aatgagtctg gagggagaga
    16561 aaatgaaaaa aaaaggaaaa gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt
    16621 tattagtagg actagctttg attttcaatg aacagatcaa ggattatttt gtcagagaga
    16681 caggagataa atatgctata gcgaatgtaa caaaagaaga tctgaaaaag aataacgata
    16741 aagatgtcag ttttgacttt gatgcggtag aaccaatgac gacagaaggg gtcatgaggt
    16801 cgcaaatgag aggaacagat ctaccagtga ttgctagtat cgcagtccct tcagtttcaa
    16861 tcaatttacc ggttttcaaa ggcttggata atacgtcact actttacgga gcaggaacat
    16921 tatctccaga tcaagaaatg ggaaaaggaa attatgcctt agcaagccat cgtgcaacga
    16981 accctgaatt gctgtttaca ccgctagaaa atttagagat gggtgccaag atctatttga
    17041 cagatttaga aaatgtgtat acatacaaaa cattttttaa agaaaaagtt gctcctacag
    17101 atacccaatt actgaatgaa gtcgagggaa aagaaattgt cacactaatt acttgtggcg
    17161 acatggacgc cgtcacgcga ctagttgtac aaggagaact ggaaagtgtt acatcgataa
    17221 aagatgcgac agatgatatg agatctgctt tcaatctaga gacaaaaaca ttttaggata
    17281 ctgggacata agtcgttaca gattaaaaac agccaaagga tatgaatcga taaggaaaaa
    17341 ccaatttgct gaaaaaaaga gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta
    17401 gatagacttg tgttccagtt tttttatttt ttatatgaaa aggcttgcat aaataaaaat
    17461 tcgatggtat gatgattttg aataagaaat ggattgttac tgtacggcag gcaaaaccag
    17521 aattcagcta agaaacagta ggcgaattct aggttttttt atttttcttg agagggcgaa
    17581 gcaaaatgaa aattgataaa attttgaata acaatgtcgt catttcgaaa aatggattcg
    17641 gggaagaagt cgtttgtatg ggaaaaggac tagcttttca aaaaaagatt ggggacgaga
    17701 tttcgccaga agcggtccaa aaagaatttg ttttaagaga ttcttttgct aaaaaccaat
    17761 tccagcagct gatggctgat attccggcag aggagatcga atgggtcaaa caagtagtcg
    17821 aactggctga agataaacta aaagttgaat tttcgcctaa tatttatctg acattgaccg
    17881 atcatcttca ttatgcgatc acacgagcaa aagaaaatat ccaactgcct aatccattat
    17941 tatttgaaac aaaaaaattt tatccgaaag aatatcaagc agcgaaagag gctcttttgt
    18001 tcgtgaaaca aaagacgggt gtggaacttt ctgaggatga agccggtttt attgcctttc
    18061 attttgtgaa tagccaacaa ggaaacgaag acatgcaagt gacgatgacc gctaccacaa
    18121 tggtaagaga tatactgagt atcatcagta aattcttcgg catcgtgttc gatgaagaat
    18181 ctttgaatta tcagcgtatc attactcatc tgcagttttt tgcccagcgt tatctcaaag
    18241 gggaaggatc tgacgagcaa gatgaatttc tctacgcatt agttcaagga aagtatccaa
    18301 aagcgtttca ttgtgtcgaa cggatcaatg agtatcttcg acaaacgcaa aaacaggaaa
    18361 tggggatcgc agagcagatt tatttaacga ttcatattca aagagtagta agtgagaaaa
    18421 aaagcatcca atcataagga ttgttactgt atggcaggca aaacctggat tgaaaaaata
    18481 ggttcacgaa tgtttgtgac tgtttttttc aatctgggtt tttatttttt aaataaaaaa
    18541 gaaagaagga aaaaacaatg gataatcaag cagttggtcg tcgtgtatgg gaagcggtag
    18601 gaggacaaaa gaatgtcaaa agtttagtac attgtgctac gcggttgcga ttcagattaa
    18661 aagatgaatc tttagcagat acgcaaaagt taaaagaaga cccggacgtg atccaagtcg
    18721 tacaaagtgg cggtcagtat caagtagtga ttggcagtaa tgtagcagat gtttatcaat
    18781 cgatcgtgga tgaggaaggt cttgcagttt cggaggatgg aaaagaagag tccaaaaata
    18841 ttttgaatcg tctgatcgat attatctcta gtatatttac accttttcta ggggcgatgg
    18901 cagccgccgg ggtattgaaa ggattcttat cattggcaac ggtgatgggc tggctgacag
    18961 ctgatagcgg tacttatcaa atattgtttg ctgcagcaga tggtgtcttt accttcttac
    19021 ctgtcatgct tgcttttaca gcagctaaaa agttcaaagc caaccagttt ttagcagtag
    19081 caattgctat ggcacttgtg tatccagcta tcacagcagt tgctggtgcg ggagaagcta
    19141 tcagtttctt tgggatccca gtgattcttt caccatctgg ttatacttct tctgtcatac
    19201 caatcattct agccgtatgg gttcaaagta aattagagcc attcgtaaaa aaagtgattc
    19261 cgcaatttct gcaaatgatt ttagttccgc ttgtcgtttt agtagtgatg gttccattaa
    19321 catttcttgc tttaggacca atcgggacgg ttgccggtaa tgctttaggc ggtctcttca
    19381 actcgattta tggatttagt ccaatcgtag caggtctaat catgggaagt ttatggcaag
    19441 tattcgtgat gttcggcatg cattggggat tcgtaccgat catgtttttg aatatcgaac
    19501 aatatgggtt tgatgtattg atgccaatgc ttcttccagc catcttggca caaggtgggg
    19561 ctgctttagc tgtagcattg cgaacaaaag atacgaaact tcgagcatta gggatttctt
    19621 ctacagtgac ttcgttattt ggaatcactg aaccaactgt gtatggtgtg acattacctt
    19681 tgaagaaacc attcattgcc gcttgtattt ctggaggtat tggtggagct attatcggat
    19741 tttccggagt aaaagctttc tccagcagtt tggtcagctt attgacgatt ccgactttca
    19801 ttaatacggt ggatggtgta gagtcaaatg tgacagtagc agtaatagct acaggaattg
    19861 cttttgttct tgcattcgta ggtacattga ttttaggatt tgacgaacaa acacaagaca
    19921 atcaattaga aaacaaacat gcgaatgcag gagaaccgat tacttcagca cggcatacct
    19981 taaaaagtcc tttaacgggt aaagtactgc cattgagtga agtaccagac caagtttttt
    20041 cttctggcgt gatgggaaaa ggtatcgcta ttgatccaga agtaggagaa ctagttgcac
    20101 ctgcagacgg agagatcacg actatttttc caactggaca tgctgtaggg atcacaacga
    20161 ctgacggagc ggagattttg attcatatcg ggatggatac agtagaatta aatgggaatg
    20221 gctttgagat cttagtcaaa caaggggatc tcgtcaaagc aggagattta ttgattcgtt
    20281 ttgacatcga agcaattaga gcagctggct atagcgtaat cactccagtt gtcattacga
    20341 atactgatgc atttgccgat atacttgaat tagaccaaaa agagattatt gcaaacgaag
    20401 atgttctggc aattgtaaaa taaggaggaa atcaggatgc aatcacgttt ttcagaaaaa
    20461 tttttatggg gtggagcaac tgctgccaaa tcaattagaa ggggcttatg atcgagacgg
    20521 aaaaggacta tccgttgcag atgctatgcc tggaggtaag cagcgctttg cgattatcgg
    20581 cagtgaagaa tttgactgga cgattgatca agaaaaatat atttacccga atcatcgagg
    20641 aatcgatcat tacgatcgtt tcaaagaaga tcttgccttg tttgcggaaa tgggattcaa
    20701 atgttatcgt ttttcgattg cctggacacg aatttttcca aatggagacg aaggtactcc
    20761 taacgaagcg ggattagaat tttatgatca gctgattgat gagtgcttaa aatatgatat
    20821 tgaacctgtg atcacgattt ctcattacga aatgcctctt catttggcaa aagaatacgg
    20881 tggctggaaa aatcgaaaac tgatcgactt ctatgaacga tttgctcaga cagtattaga
    20941 gagatacagc agtaaagtca aatattggat gactttcaat gaaatcaatt cggcctttca
    21001 ctttccagct ttaagtcaag gattagtaaa aagcaatgga gcaggagagt accaaaatat
    21061 tttccaagca tggcataatc aattcgttgc aagtagcaaa gccgtaaaaa tcggccacga
    21121 attgcgttcg gatatccaaa tcggctgcat gattatctat gcaacaactt acagcatcga
    21181 tgccaatcca gtcaatcagg cagcaacaat gatccaaaac caagaattca attttttctg
    21241 cacggatgtc caagtacgtg gagaataccc tgcatacaca gcgcgtaccc ataaaaaata
    21301 tggagtcgat ccggaaaaat tagagcaaac ggaaaaagac ttccagttgc taaaagaata
    21361 tccagtggac tacatcggat ttagttatta tatgtctacg gcaatcaatg agactgatcc
    21421 acaagcagca acttcagaag ggaacttgct gggaggagta aaaaatccgt ttcttgaggc
    21481 aagtgaatgg ggctggcaga tcgatccaga agggctgaga atcgcattga atgaattata
    21541 caatcgttac cagaaaccat tattcattgt tgaaaacggt cttggagcca ttgatcatat
    21601 ggaagaggat ggcacgatta ttgatgatta tcggatcgat tatttacgtc gtcatatcga
    21661 agcgatggct aatgcagtag cagacggtgt agatttgatg ggctatacgc cttggggatg
    21721 tatcgatctt gtcagtgcat caacaggaga aatgagtaaa cgttacggct ttatctatgt
    21781 ggatctagac gatgaaggaa atgggacact gaatcgatca aagaaaaaat cttttgattg
    21841 gtataaagaa gtaattgcct caaatggcgt aaacttgtaa ttaaagcagc tgaatgaaaa
    21901 agaacacact tctcacatgt gatttgtgtt ctttttttat tttttatgtg ctattctttt
    21961 agtaatagta aagcaaaggg tgacgttatg aaaaggacac gtgtactata cttggaagtc
    22021 gcagataaaa ttaaagaaga tattttctca ggaaaatatc cagtcggttc gatgttgccg
    22081 acagaaagtg aattagaaga actgtttcaa gtcagcaaaa tcactgtccg aaaggcaatc
    22141 gaactgctgg catcagatga atatgtcgca aagaaaagtg gtcgtgggac gacggttcta
    22201 agcaatcgtc cttacaatcg attatccaaa gcggcaagct ttacgcaaat cttggagaac
    22261 tcccgatatg aagtccggaa agaaacgctg gatttgcaat tggtccatat aaagaaggaa
    22321 gatcctttgt tttccttttt cggtgagcaa gctgtttgtt ttaagcgctt atattatttg
    22381 aacaatcagc cttatattta ttttgttcat tatcttccac aagaaatgat gcaattcagc
    22441 agagaagaat ttggcaaaga atctttgtat cgtttgctga cgaaaaaagg atatactatt
    22501 gactcatttt ctgatgattt ctcggcagta tttttaacag aagaagaaca aggaattctg
    22561 aaaacagacg cacagatcgg catcaaacgg atacggaaat cgtctacgca aattggggaa
    22621 atgattgaat attcggaggc ggtctaccat acggagctgc atccttacca tattgattat
    22681 gaaacataaa taaaaaaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
    22741 ccaaacaaaa atagcttctc atatttttcg tttggttagg cttattaccg cagatacacg
    22801 gctgtttctg gattaccatt tattctggat aagggggtgt ggcacgattt tcagaaggaa
    22861 tctatgaacc aatcactgta ttgattttag cttcgatggt aaccccattc ggcttgacga
    22921 tcgcttattt cttagggaaa atcattcgta aaaatatact caatcgtcaa gaaatcgata
    22981 cattgaagac cgcttttcct atggggatct gtcagatcac agagggatgt ttccctatcg
    23041 tattgaacga cttggcacgc aatgtcatag caacgggagt cggtgggaca gtaggtggcg
    23101 gattaagcat gttttggggc gcagatagcc gtattccggc atcgggaatg tttgcagtcg
    23161 ctacgatgac cagaccatgg gcattcattg gtgcattgtt agctggttct tttgtcacag
    23221 gaataaccat tttattattg aaaaaaccag tagatccgaa tgcagaaatc atccaagaag
    23281 aaaaagagga agaagacata tcttgggatg atttgacaat tagttaggag aggcagaatg
    23341 gattacaaga aaaaagtaca atagatgtac cttgagacag gaatggtatg aaagaatcag
    23401 agttagaaag aatcgaatat acagattttg gtttaggagc gatcgaaact gtccagagca
    23461 tcggcaccct ccttgaaatg acgatgaagg aaaaatgaga acctttcgtt gtcgaaaggg
    23521 tctggtatac cttatgtcga aggaaaggaa acaaaaaaac tttcctgtac tattccaaaa
    23581 aggaaagaaa gttattatac agtaaaaaaa gaaattattc ttcatgctag agatcagtac
    23641 acgttcgagc ccaatatcaa acactagttc caggcaggcg caacaggtgc ggttatcact
    23701 gaattttctt ccagcagtga cgattcaagt gatattttta caaatccagc tatttatcgt
    23761 taacaaaaag atttttagga gaagctgatg aacaaaaatg atcaaatttt gatcgtttag
    23821 ttcgtcggct tttttattta gagaaacatc atttatcagt gaaacataac acgagaacaa
    23881 aatgattttt tcgttaataa aagaaacaag tatttgttgt ttaaaataat acacgttatt
    23941 ggcatatagg acgaaatccc agtatcttct ataagtaagt catttcatat cgatgaaaac
    24001 acgttattta tttttgtttt atctataaaa tggctgaaaa atgatttttt tctaaatact
    24061 atatatagtg gcgtctatcc tatataccac taaaaaacta caaaatctag tcttttattt
    24121 taaaaaaaca cgtgtagaat gaagatggac ttttgagtta gggagtgttt ttttatgaaa
    24181 cttactgttt tttgcggttc ccgttttggt aataaagaaa gttacaaatt tattgcgcaa
    24241 acgttaggaa aatacatggc acaagaagaa atcgaactgg tttacggtgg ttcatactcc
    24301 gggattatgg gagtgattag taaaacagta ctggaaaata acggaaaagt cacaggtatc
    24361 tatccgaatg ggctttttga aactgaattg cctggaaaag atgttactac atttattcct
    24421 acagaaacga tcgatgaaag aaaggtatta ttatttgaaa agggagatgc tgtcctcgtt
    24481 ttccctggag gtctaggtac attggaggaa ttctcacaac ttctttcttg gatagcaatc
    24541 ggtctgacgc ccgacaagcc aatcggaata ttgaatattg gcggttatta cagcggcttg
    24601 caacaattat tagaaacttt tgcaaaagag gattttatgg ataaaaaatg gttggatcaa
    24661 gtaatctttt ccaacaatcc attggaactg gtcaatgaat tacgagcagt atctattaaa
    24721 aatcagttat tattgaagga ggcaaaataa tgagtcaaat atatttagca ggaccatttt
    24781 tttcagagga gcaaatcgat cgagtaagtc gtatcgaaaa agcgttggag gaaaacaaaa
    24841 cagtcacaag tttctactct ccacgtcatc accaagaaag caattatgaa ctattcagcg
    24901 ctggatgggc gcaggaagta tacgaaaaag atatggaaga actgacaaat gcagaatttg
    24961 ttgttgctat ccttgacttt gagcatcaaa caatcgatcc aggaacggct tacgaattag
    25021 gtgtggcaac tatgttgaaa aaaccgatga tcattgtaca agaagagacg gtacctacga
    25081 acttgatgat tacacaaagt ctccatacgt atttaaaatc agaccaagct gtacgtgaat
    25141 atgattttga aacattgcct gttgaaactt acgtaggcga gtacttatag aagaggaagg
    25201 gttgtatgtc agtgattttg atttcaggaa cgattggtgc aggtaagagc agtttgacgg
    25261 atatgcttgc aaaagagatt gattctaaac cattttatga aaatgtcgaa gataacgaag
    25321 tattgccgct cttttattca aatcctgagc agtacgcttt tttattgcaa atcttctttt
    25381 tgaacaagcg atttttagca atgaaaaatg ctttagtgaa cgatgacaat gtgctagacc
    25441 gatccatcta tgaagatagc ctgttgttcc atctaaacgc tgatttaggt cgggtaacgg
    25501 atatcgaagt acagcagtac gatagcttat tagatacgat gctaaacgag ttagatgatg
    25561 ttgctccaaa aaaacggcct gatttgatgg tccatatcaa agtttctttg gacacgatgc
    25621 tagaacgtat caaaaaacgt gggcgtgact atgaacagct agaaagcgat gagacgttat
    25681 atacctatta tgaaacgttg aacacccgct ataatcaatg gtatgaagat tttgatgtat
    25741 gtcctaaaat acaagtggat ggggataaat tcgattttgt agaaaatcca gaagatgcaa
    25801 aaaaggtgat ccgtcagatc caagaaaaat tagctgaatt ggaaggaaag cctgctggaa
    25861 aatattttac ggataaccag catgcaaaag gtcaagaatt aggctaaaaa taaaaaacca
    25921 aacgacggga aagaaaaatt actacgtgat gtagaataag cctttctatc gtttggttat
    25981 tttatattca aatatttgaa ataaataaat ctttactctt tgatttgtaa actgaaatga
    26041 tccactgttt gttttactga tgaagcttga tcaaaggaag aagctgagtt tgaagttcat
    26101 aaaaatcagc catagtgagt tttgcttgga tttgtttatc ttcttctact ttttacataa
    26161 ttttgaacag gataagattc aaatgtttta aatgaatctt tgactagttt gtgacagttg
    26221 tcgcatatgc tcaagcttcc ttggtcatta aattctgtat ggatattttt gttgttatta
    26281 catgtacaat aatcaggact atcgatcatc catttcgtct cctttacaat catcttgctt
    26341 ctttactata gcagaaaaga aggggagaaa caaaagaaga tccaacagac ggcactatag
    26401 actgtagatg ttgccatcct catccttcaa acggtaatta ccgatttttg taaatatgac
    26461 tgttgtttct tttttatttt cttttcctga aataatatat tcgtcatctt tggtagtgat
    26521 cttgatatct gtcagcactg ttttttcttc agatgattca gaagaatcag ttccttgtgt
    26581 ctgatacgtg cgtgtccatt ccattttttt atctgtaaat gtcaactgtc ccgcggataa
    26641 atccatatct ttacgtactt ttagcgtata tacactgttg gtggtatctg ctttttccgc
    26701 ttttaagaca gaagaatcgg ttgtttcgtc cgaggattgg tcagagccgc caaacaaatc
    26761 acagccagtc aatgtcaaaa gtcccgcaga caaaataaat agtacgatta atttcttcaa
    26821 aatacttccc ccatttgata ggataaagaa attataccat tggaatgtaa aacttcaaac
    26881 ggatggaaga gaaaaacgca caaaacaccc acaaatagag taaattttag aataaaattt
    26941 taaaaaggca aaaaatccgc cactttcaga aagcgtttta aaaaagtgtt gcaatctttt
    27001 aaaaaaatgc tatattaaaa atgaaccggt tcaaatactt gtttaagtat aattaatagt
    27061 gagaggaagt tgctctactg aagactttag ttatatggat attgatgaca agcaacacta
    27121 tgcaaagaat gcggtcatca taatcgaaca acaagaaaga gaaatcaaat gtaactggca
    27181 ggagtgagaa tgtatggcat cgatccgtga tgtggctaag atggctggtg tttcagtggg
    27241 aacagtttcc cgttacttga atggtcagca actgaaggaa aagaacatga aaaaaatagc
    27301 agaagcgatt tcagcgttgg actataaaga aaacatcatt gctaaagggt tgaagaataa
    27361 tcgaagcttc tctattggac ttttgataaa cgggatttcc agccgttttg ggtcggaagt
    27421 tgtctcaggg atcgaaagag tagctgaaga aaatgggtat agcttattat taagcgggtt
    27481 tgccgatcag ccagaaaaaa ttgaacaaaa aattgaatac ctgatgaagc atgtgatcga
    27541 tgggctgatt gtcttcttat ccgaagaaga gtggtctgga tttgaaatgc tcacaaaaat
    27601 gagtattcca gttatcgcat tgaattgtcc agaaggacca gcaggtattg atacaatatt
    27661 agtgaatgat cgagaaagtg tagcaaaagt gatcgctcat catggtgaac aaggacataa
    27721 aaaaatcgga tttattgctg ccactcaaac agactacgta gcaagagaac gtttagcagg
    27781 agccaaagaa gcggtccaaa atgatccatc gatccaatta gaggtcttta caggagacta
    27841 ttctagaaaa agcggctatt acggggcaaa agcattatta gaaaaagggg caactgctat
    27901 ttttgtcagc aattacaata tgtcgattgg tgccatcgaa tgtttcaatc aagaaggtgt
    27961 cagaatcggg aaggatattg cgttcagtca ctatgattat ttagataaaa acgatctgag
    28021 tatccttccg aaaatcacga tcacgccacc aacagataaa atcggggaac gagcagcaga
    28081 acagctgtta aaacgaatca aaaaagaaga ggaagaaaat gaaaaagtga ttgtgatgga
    28141 aaatatcata catggaatca actaaaacga actaaaaaag aaaaagaggt gctttgatga
    28201 aaaaatatca atttccagca aatttttggt ggggttctgc agcaagtggc ccacaaacag
    28261 aaggaaggat ttcaggcgat ggaaaaggag agaacatttt tgattattgg tacaaaaaag
    28321 aaccgcaaaa gttctttgat caagtaggac ctgaaaaaac ctcgcaagtt tatacgaagt
    28381 atcaagagga tgtacagttg atgaaacaga ctgggcataa ttcatttcga acatctatcc
    28441 aatggagccg tttgatacct gatgaatctg gaacaatcaa cgaagaagcc gttcgtttct
    28501 atcatcgtta ttttgatgag ttgattgaaa acggtatcga accctttgtg aatctgtatc
    28561 atttcgatat gccgatgtat ctgcaagaaa aaggcggatg gttgaaccga gaaacggttg
    28621 aagcctatga aaaatatgcc aaaagttgtt ttgaattgtt tggttcgaaa gtaaaaaaat
    28681 ggtttactca taatgaacca atcgttccag tcgaagcagg ttatttatat cgatggcatt
    28741 atccagaaga gtcagatatg aaaaaagcga tccaagttgg ctatcatgaa gcgttggctt
    28801 cagcgttagc catcaaatct taccatgaaa tggagcaagg tggtgttatt ggtatcatct
    28861 tgaatctgac accaagctat ccaagagacg aatcaaaccc tgaagatgtt aaagcagcgc
    28921 aaatagcaga tgcgttcttc aatcgttcat tccttgatcc agctgtaaaa ggcgagtttc
    28981 caagtgaatt gattgagata gtaaaagaac ttgatctcat gcctgtgatc catgcagagg
    29041 atttgacggt tatcaaagaa aatacagtag atcttttagg tatcaattat taccagccac
    29101 gccgggtaaa agcaaaagaa acaccaatcg atactaaaaa cggtccaatg cctgaggatt
    29161 atttcgacaa ttatgaaatg cctggtcgta aaatgaatcc ttatcgcgga tgggagatct
    29221 atgaacaagg aatctatgat attttgatca atgtacgaga tcattatgga aatatcgact
    29281 gctttatttc tgaaaacggt atgggtgtag aaaacgaagc acgatttatc aaagaagatg
    29341 gcatgatcca cgatgattat cggatcgaat ttgtccaatc tcatctgaaa tatgtccatc
    29401 aagccattca agaaggtgcc aattgtttag gctatcatat gtggacatgt atggataact
    29461 ggtcgtggac caatgcatat aagaatcgat atggctttat ttcagtagac ttagcacaag
    29521 atggaaaaag aacaatcaaa aaatcgggtc attggttcaa agacgtatca gataaaaacg
    29581 gatttgaggc ttaagaaaga atagttgaga gcaaatgcgt gaaattctcc attcttacca
    29641 ttttgtgaaa aagaagttca attatttacg atctttttca acttttttgt ctttctattt
    29701 cattttttgt atataataga atagtcaaaa ttaaatagga gtggaggaaa gcatgaggaa
    29761 aaaaagaccg tttcttctct tttggttatt cggttttttc ttactgctta ttttatccgc
    29821 agcattttca ggaaaaatga cagcagatct tgctcagaaa gaaacagaaa aaatcgtagc
    29881 aagcaaagaa tcagtagata atttcaaaaa agaattctca acttattggt gggcggagta
    29941 taaaataact gccaattacg aaaaaggcga attggacatt tatttgccaa aaggaaaaga
    30001 agaaaatcaa tttattaata tcgatgacgt acttagtgcg atttctctca gacaatatca
    30061 atttaaagac aagcatttga ccgttttgct tttgactcat gatggcgaca aagtagcccg
    30121 tttgaaaaat ggaaaagtga tcaccgctta ttctgaaaaa gtagacaaaa aagtggtcgc
    30181 cgatcgttta gcggaatgga aaaagacaaa tgaacaagtc aagaaaacaa ctgtctcttc
    30241 tcaaaaaaac gagtcgacag gaacaagtcc ttcagattct ggcagtggac aaacgataac
    30301 gggaagtgaa gaaaatcaag cctcaattat tacgaatgag tcatcacaag gacaatatca
    30361 atcctatgga caatgaaaaa cagaggttgt gaaatcagcg ttttgacctg agtattagag
    30421 aaacaaacgg caaaat
//
LOCUS       DACEOM010000035        29107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEOM010000035 DACEOM010000000
VERSION     DACEOM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.036"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1031)
                     /locus_tag="IW781_001471"
     CDS             complement(<1..1031)
                     /locus_tag="IW781_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1182472.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IW781_001472"
     CDS             complement(1113..1802)
                     /locus_tag="IW781_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1182473.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IW781_001473"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IW781_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1182474.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IW781_001474"
     CDS             complement(2329..2832)
                     /locus_tag="IW781_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1182475.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IW781_001475"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IW781_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1182476.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IW781_001476"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IW781_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1182477.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IW781_001477"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IW781_001477"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1182478.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IW781_001478"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IW781_001478"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1182479.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IW781_001479"
     CDS             complement(6444..7463)
                     /locus_tag="IW781_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1182480.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IW781_001480"
     CDS             complement(7636..8523)
                     /locus_tag="IW781_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1182481.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IW781_001481"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IW781_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1182482.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IW781_001482"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IW781_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1182483.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IW781_001483"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IW781_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1182484.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IW781_001484"
     CDS             9754..10398
                     /locus_tag="IW781_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1182485.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IW781_001485"
     CDS             complement(10454..10957)
                     /locus_tag="IW781_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1182486.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IW781_001486"
     CDS             11554..12519
                     /locus_tag="IW781_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1182487.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IW781_001487"
     CDS             complement(12618..13790)
                     /locus_tag="IW781_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182488.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IW781_001488"
     CDS             complement(13777..14922)
                     /locus_tag="IW781_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182489.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IW781_001489"
     CDS             complement(14936..15868)
                     /locus_tag="IW781_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182490.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IW781_001490"
     CDS             15985..17064
                     /locus_tag="IW781_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1182491.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IW781_001491"
     CDS             17061..17690
                     /locus_tag="IW781_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1182492.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IW781_001492"
     CDS             complement(17800..18027)
                     /locus_tag="IW781_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1182493.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IW781_001493"
     CDS             complement(18130..20718)
                     /locus_tag="IW781_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1182494.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IW781_001494"
     CDS             complement(20738..21397)
                     /locus_tag="IW781_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1182495.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IW781_001495"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IW781_001495"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1182496.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IW781_001496"
     CDS             complement(22170..23036)
                     /locus_tag="IW781_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1182497.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IW781_001497"
     CDS             23145..23786
                     /locus_tag="IW781_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1182498.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IW781_001498"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IW781_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1182499.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IW781_001499"
     CDS             complement(25227..26126)
                     /locus_tag="IW781_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1182500.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IW781_001500"
     CDS             complement(26128..26925)
                     /locus_tag="IW781_001500"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1182501.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IW781_001501"
     CDS             complement(26903..27607)
                     /locus_tag="IW781_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1182502.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IW781_001502"
     CDS             27738..28313
                     /locus_tag="IW781_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1182503.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..>29107
                     /locus_tag="IW781_001503"
     CDS             28431..>29107
                     /locus_tag="IW781_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1182504.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6169 c         5232 g         9143 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaatta
//
LOCUS       DACEOM010000036        28780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEOM010000036 DACEOM010000000
VERSION     DACEOM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.037"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(152..967)
                     /gene="thiD"
                     /locus_tag="IW781_001504"
     CDS             complement(152..967)
                     /gene="thiD"
                     /locus_tag="IW781_001504"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1182505.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1261..1536
                     /locus_tag="IW781_001505"
     CDS             1261..1536
                     /locus_tag="IW781_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182506.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            1963..2418
                     /locus_tag="IW781_001506"
     CDS             1963..2418
                     /locus_tag="IW781_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1182507.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(2498..3247)
                     /locus_tag="IW781_001507"
     CDS             complement(2498..3247)
                     /locus_tag="IW781_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182508.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(3248..3574)
                     /locus_tag="IW781_001508"
     CDS             complement(3248..3574)
                     /locus_tag="IW781_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1182509.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            complement(3722..4162)
                     /locus_tag="IW781_001509"
     CDS             complement(3722..4162)
                     /locus_tag="IW781_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1182510.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            complement(4243..5640)
                     /locus_tag="IW781_001510"
     CDS             complement(4243..5640)
                     /locus_tag="IW781_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1182511.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            5794..7065
                     /gene="celB"
                     /locus_tag="IW781_001511"
     CDS             5794..7065
                     /gene="celB"
                     /locus_tag="IW781_001511"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1182512.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7132..7911
                     /locus_tag="IW781_001512"
     CDS             7132..7911
                     /locus_tag="IW781_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1182513.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            8289..9263
                     /locus_tag="IW781_001513"
     CDS             8289..9263
                     /locus_tag="IW781_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1182514.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            9316..9921
                     /locus_tag="IW781_001514"
     CDS             9316..9921
                     /locus_tag="IW781_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1182515.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(10015..10767)
                     /locus_tag="IW781_001515"
     CDS             complement(10015..10767)
                     /locus_tag="IW781_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1182516.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(10781..11041)
                     /locus_tag="IW781_001516"
     CDS             complement(10781..11041)
                     /locus_tag="IW781_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182517.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            complement(11063..12424)
                     /locus_tag="IW781_001517"
     CDS             complement(11063..12424)
                     /locus_tag="IW781_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1182518.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            complement(12497..12796)
                     /locus_tag="IW781_001518"
     CDS             complement(12497..12796)
                     /locus_tag="IW781_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1182519.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            complement(12809..13267)
                     /locus_tag="IW781_001519"
     CDS             complement(12809..13267)
                     /locus_tag="IW781_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1182520.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITNYI"
     gene            13491..14282
                     /locus_tag="IW781_001520"
     CDS             13491..14282
                     /locus_tag="IW781_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1182521.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            14248..15201
                     /locus_tag="IW781_001521"
     CDS             14248..15201
                     /locus_tag="IW781_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ1182522.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(15599..16573)
                     /locus_tag="IW781_001522"
     CDS             complement(15599..16573)
                     /locus_tag="IW781_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1182523.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            16688..17122
                     /locus_tag="IW781_001523"
     CDS             16688..17122
                     /locus_tag="IW781_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1182524.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(17247..18233)
                     /locus_tag="IW781_001524"
     CDS             complement(17247..18233)
                     /locus_tag="IW781_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAQ1182525.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            18661..19083
                     /gene="arsC"
                     /locus_tag="IW781_001525"
     CDS             18661..19083
                     /gene="arsC"
                     /locus_tag="IW781_001525"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ1182526.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(19363..21285)
                     /locus_tag="IW781_001526"
     CDS             complement(19363..21285)
                     /locus_tag="IW781_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ1182527.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(21372..23312)
                     /locus_tag="IW781_001527"
     CDS             complement(21372..23312)
                     /locus_tag="IW781_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1182528.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(23600..24340)
                     /locus_tag="IW781_001528"
     CDS             complement(23600..24340)
                     /locus_tag="IW781_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ1182529.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(24353..25444)
                     /locus_tag="IW781_001529"
     CDS             complement(24353..25444)
                     /locus_tag="IW781_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ1182530.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            complement(25434..26540)
                     /locus_tag="IW781_001530"
     CDS             complement(25434..26540)
                     /locus_tag="IW781_001530"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ1182531.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            complement(26559..27236)
                     /locus_tag="IW781_001531"
     CDS             complement(26559..27236)
                     /locus_tag="IW781_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ1182532.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AILPAVN"
     gene            complement(27247..28026)
                     /locus_tag="IW781_001532"
     CDS             complement(27247..28026)
                     /locus_tag="IW781_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ1182533.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            complement(28055..28405)
                     /locus_tag="IW781_001533"
     CDS             complement(28055..28405)
                     /locus_tag="IW781_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ1182534.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            complement(28421..28777)
                     /locus_tag="IW781_001534"
     CDS             complement(28421..28777)
                     /locus_tag="IW781_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182535.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
BASE COUNT         8631 a         5963 c         4892 g         9294 t
ORIGIN      
        1 ggacggcagg ctcacgacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat
       61 aagtccaacc aagttaaaaa cataagaagc tgtttttact tggctgttct tattacttga
      121 tgcagggcaa ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat
      181 tgatggggcc atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt
      241 cttttgccaa tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac
      301 atgcagatag cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta
      361 accaaaatga ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat
      421 ttttagcatg atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg
      481 ctgcttgcac aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat
      541 aaaaattagg tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag
      601 cagattcttc tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg
      661 gtccaaaatc atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta
      721 gcatacctgt tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt
      781 cgataaaatc tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac
      841 atgcggtcat gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt
      901 gcatccctgc gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat
      961 cttccatcaa atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt
     1021 tctatcatat gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat
     1081 caaatttatt tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt
     1141 cgctattcta ataataaata accactattt gatttatata tatttcagta tccttcttgt
     1201 tttcatataa ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa
     1261 atgaaacttg aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata
     1321 gatggctata cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac
     1381 acagaaaaag gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg
     1441 tttactgaaa aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatttcca
     1501 agaagtcata aactctatgt gaatcagcaa ttttaacttc taactgttct ttattaatta
     1561 aaaatgttct gtaataaaat aaaactttat aacaactata acaaaaccga atgcaaaaca
     1621 gaagttctat aagttataat catagttgtt aggtgattca tctagtaaaa acaccagatt
     1681 tcaaatttca cacaatttaa actagatcaa tcatcaacct tcttcaaatt gttagaaccc
     1741 tttcccgagg agctgcagca taagcaaaaa aacaaacagg aagccaaatg gctaagacat
     1801 tttttctatt agtctagttg tttctcgtcg caagtttaag actttagaaa gttatttcta
     1861 atgatgtttg ggttgttttg atcaatgctt atgcacagct ccttacctat tatttatctc
     1921 aacagatcta acaaattaat ggactattta aggagttttt ttatgcttac gatctattct
     1981 tctcgcacac atagttacca tctgatggca aactggctga aagaccatca aatgccttac
     2041 agagaacgaa ttttcacacc taaacagcct ttaacaagga aagagatatt ttatttattg
     2101 tcattaacag aaaacggatt tgacgatcta ttagctaaac gctcgattga ttataaaaat
     2161 ttgaaagacc aaatcgacca atgtacgacc tcagagcttg tcgatctgat cgtctcgcat
     2221 tttactttgt tacaacgtcc catcattact gacggaaaga aattattagt cggattcaat
     2281 gaagatagca tgcgtctttt tatccctgta caatggcgca gaagtcaact gaaatctgtc
     2341 tataacgatt ttacatgggt aagcaatcta gaaaattcta cggatttaga tgatgaattt
     2401 ttaaattatt atatataaaa aagactgtga caaaagcgtg tcacagtctt tttttctacg
     2461 ccggataaat agaatatttt tacaaatctg aatacattca ccagtaataa gcgctttgag
     2521 tgaattgacg ataagaatga cggactagac tgccataaat gatcaagaag acacccgttc
     2581 ctaccgtaac aaaaaatata gaggcaccga tactaccatc catataccac attaaagaag
     2641 ccaaaagtgg gaaaagtgaa aaaatacata atccaattcc tgcaatcagt gagaagccaa
     2701 aacttcgctg atactcttct tctttttctt ttgcttctgc taatactttt tcaggtattt
     2761 tccgatggtg caaaaagtga tttaaacgtg tgattttcat accagaagca ataaaaaaac
     2821 cgacagctaa tgctataccg aaaatcagac aagaaatacc catgaacaca tatcctgctg
     2881 aacaaaagaa aagaaaactt gcaaaagata agctaatgaa tagaacacct gatgccactt
     2941 ggagacttgc cgctctgtat tctttccagt agttttctgc ttcgcttaaa taaatagaag
     3001 ctgtgtttgt ttccgtctcg tcagcttgat gttttttttc gacatctagt tcttctaata
     3061 attcatcgat tgaaccaaat gtcgaaatta ctgtaccgat ggcctcctgt tcattttttc
     3121 cttctcccat caattcatga taatggtctt ccatatttgc caacaaatca gttttcagct
     3181 tatctatctc ggtactttga ggtactccga taaaaagact gtctaaataa ttacggattg
     3241 tatccattta atttccctcc aaaaattgat cgactacgtc tttcgtcgtc agccactctt
     3301 gctttttttc ttctaaaaga ttacgcccta tttcagtgat tttataatat gtccgctttt
     3361 ttccatgagt cacttctcca ggatacgaaa caatcagttg attcttttct aaacgggcaa
     3421 aaacggagta aagtgttgtc tctttgatca cataggaatc atttgtcctc tcacgaatcg
     3481 ttttagaaat ttcatatcca taagaatcgt ttttgttaag gatcgataaa ataatcgcat
     3541 cattgtatcc gcgtattcca tcacttgaaa tcattttctt tcctccatat tactccatct
     3601 gtcgttgtaa ttagaatagc atgattactt catcaggtaa agtaattttt tacaaaaaaa
     3661 taaaaggaac agaaagcaaa agaagcgtta tgcttttttg ctttctgttc ccttaattta
     3721 tttattttac aaccatgacg ttacatgatg cgtggttgac tacataggaa gtagtggaac
     3781 ctactagtgc acgttctacc gcattcagac ctgtcgcccc catgacaatc agatcgattg
     3841 gttctttcga ttcatggaaa ttagcaatca gtgtctttgg ataaccgtaa agaacatatg
     3901 tctcgatatc tgtcaggcct ttttcaattg ccaagtttcg atgttctttc atctggtttt
     3961 ccgcaacctc atccaattct tcctgaaggc ttgttgtaac aaatgcaaaa tcaccaaagt
     4021 aattgcctgt tacttcattg acatacagga tgtgcaacgt tgcctgattt cgtttggcaa
     4081 tcgtgattgc ctcaagaaat gctttttctg ccttttcaga accatcaatt gctactaaaa
     4141 tattacgata agtatctacc attgtaaacg cctccttcac tgttagtgta gcacgattac
     4201 agtgttagaa caaacatttt gtgaacgttt tatcaaaaaa tatcaggcat caaatccgtt
     4261 tttttcagag accgttttaa accagcgacc tgattttttg atcgtccgtt taccgtcttg
     4321 tgccaaatca acagcgataa agccatagcg attcttataa gcatttgtcc aagaccagtt
     4381 gtccatacat gtccacatgt ggtaacccac acaattggtt ccttcttgga ttgcttgatg
     4441 gacgtatttc aaatgatccg taacaaactc gatccggtaa tcatcttcaa taattccttc
     4501 gctgttaaca aatcgttctt ctccttcgac ccccatccca ttttcagaaa tatagcattt
     4561 gatattacca tagttttctc ttgtattcgt taaaatatca tagattcctt tttcgtaaat
     4621 ttcccatcca cgatatggat tcattttttt attgggcatg tcgtaattat caaaataatc
     4681 atctggcatg gggctatgtt cgtgattgat tggagtttct tttgctttga ttcgacgagg
     4741 ctgatagtaa ttgattccta gcaggtcaat cgtgttttca cggatgagtt ctaggtctcc
     4801 ttcatggatc tctggcatta tagccaattc tttgacaatt tcgatcaact cttgtggaaa
     4861 ctctcctttc actgcagggt caaggaatga tcggttgaag aatgcgtcag cgatcttcgc
     4921 cgcttttaca tcttctgggt tcgtttcatc tctcggatag cttggtgtca gattcaagat
     4981 gattccgatc tcaccatcat gtccgccttc atggtaggct ttgattacct ttgcgcttgc
     5041 tagattctca tgatagccta cttggaccgc ttctttcata ttgatatgat ttggatagtg
     5101 gaattgatac aagtagcctc cttctacagg tacgattggt tcattatgag taaaccattt
     5161 tttgaccaga tctccaaaca gttcaaagca cgctgctgca taagaaacat aagcgtcgac
     5221 cgtttctctt gataaccatc cgcctttttc ttgcagtggt agtggcatat cgaaatggta
     5281 aaggttcaca aaaggttcaa taccattatc cagcaattca gtaaaataat ctcgataaaa
     5341 agaaacagct ttttgattga tttcacctgt cccttctgga atcaaatgac tccattggat
     5401 agatgtacga aatgtgttat ggccagtttc tttcattaac tgaacatctt ctttgtattt
     5461 tgtgtaaact tgcgaagttt tttcaggtcc tacttgatcg aagaattttt caggctctaa
     5521 ttcataccaa tgatcccaaa tattttctcc tttaccatca tcacttactc ttccttctgt
     5581 ttgcggtccg ctagcagcag aaccccacca aaaattttct gggaatgtgt aggttttcat
     5641 ttttttactt cctctctttt ttcttgatac cgtaatcata acgaacccta aaaaataaat
     5701 caatactttt ttatgaaaat gactagacat ttaaattttt tggcgttata ctttgtatgt
     5761 aatcgattgc aaataaattt gaggtgaata aaaatgaacg gttttatgga caagctttct
     5821 gagaaaatca tgcccttagc taatttatta gggcaaaatc gttaccttac agtgctgcgg
     5881 gatgctttca tgctttcctt ccctttaacg atgttcggtt cgatcgttgt cgttatcaat
     5941 aacttgcctt ttttcagtga cgcaacaaag ggaactttat cgaatttatt cggtaatggg
     6001 caaaatgcaa caatgagcat catgtcggtt tttgttactt ttggtatcgg ctattattta
     6061 tcaaaatctt atgacgttga aggtattttc ggtggtgccg tgtcttttgc cagcttcttg
     6121 attcttacgc cttttgcaat gaaaaccgct ggcggtgaag atgtgactgg tgtgctttca
     6181 cttgatcgtc ttggcgcaaa aggaatgttc attggaatga tcgctgcttt cttggctgga
     6241 gaaatctact gcagaatcac gaaacgcggc tggcagatca aaatgcctga tggagttccg
     6301 cctgcagtaa caaaatcatt tgccgctttg atccctgctg tagtgacact aactattttc
     6361 ttagtaatca atgctgtgat gactggtatt ttcaatgcta acttacatga cgtcgtttat
     6421 gaagtgatcc aaaaaccatt aactggtttg ggaagtagtt tacctgcaac attgattgct
     6481 ttgttctttg tccaattctt atggttcttc ggacttcacg gacaaatcat cgtcaactct
     6541 gtaatggacc cgatttggaa tacactgatg cttgataact tagaagctta tcaacatgga
     6601 aaagaattac cacacatcat cacaaaacca ttcatggaaa ccttcactgt tggtattggt
     6661 ggctctggaa tgactttagc tgtcgtttta ttgatggcct ttgttcttaa aaagaaacag
     6721 tatcgtgatg ttggacgatt agctttagca cctggtattt tcaatgtaaa tgaaccagcg
     6781 atcttcggtc taccgatcgt attgaatgca acaatcctta ttccttgggt tgtggcacct
     6841 ctaatcgtaa cgacgttcaa ttatttggta atggctgctg gacttgtccc tgcaccaact
     6901 ggcgtctctg ttccatggac tgtgccaatc attgcaagcg gtgtcttagc aacaaattca
     6961 tggcttggtg gtatgctgca agtcatcgac tttgtcatcg ttgcctttat ctggtaccca
     7021 ttcctgaaag tactagataa acaaccagat ttagatattg cttaatagat cattaagggg
     7081 ttgtgacaaa aacttgtcat gacctctttt ttcgaataaa caatgttcca gatgctactt
     7141 cgagttgtcg gaagcataat ctttcgttat actctagaag aggtgaataa cgtgccaaaa
     7201 tacgaagaaa tcgcaaatat tttaagagaa cgtatccgtt caaaagagta tcctctcgat
     7261 agttttttgc ctaatcaagt cgacttggta aaagaatttg gtgcgagccg gatgacaatc
     7321 aaaaaagcca tcaatatctt agcaatggaa ggacttgtct attcgcagcg aggatcagga
     7381 acgaaaatct tgaatcatcc atttatgcaa aaagacacat cgcccgctaa cgagtatgaa
     7441 ggtttgagtc tccaaatgaa acgcagacat cgttctttga agagtcaaat cattcatttt
     7501 gatgttgaat tccctaatca atatatccaa gaacgattga tgatcgatgc cgagcaacct
     7561 gtctacaata ttcaccggtt aaggatctta gatggcgagc catatgtttt agagcatacc
     7621 tttatgccag tccatcttgt gccgggttta aaaaaagaac atttgttagc ctctatctat
     7681 gatcatcttc atcaagaatt gaatgtccat tttgcagggg cttatcgtgc tatttctgca
     7741 gataaaagtg atgaatatga tcagcaatat ttgaactgtg cagaaaacga tcctgtactg
     7801 gaagttcagc aagtcgtcta tctcaaagat ggacagccga ttgaatattc ttccagccga
     7861 aaccgatttg atgttcggaa ctattctgtg ctggatgtgc gttcaactta accaaagagg
     7921 ttgtgaaaaa gccgtttacc aagagatcgt gacagtggta agcagtttca agtaacaaga
     7981 agagatcgtg aaatcaacat tctattctat cctaagcatt agctcaaaaa tgaaaaaata
     8041 actcagcata aaaagactta tgaccacccg taatgattct aaagttagct ttttaaagct
     8101 agtttttttg aaatcgatac agctagtcgt aagtcttttt attttattga tccgtccctc
     8161 ctcttttcca ttcttgattc cttcctgata ctttagaaga caaattaatt gcaaaataga
     8221 gatgcaccct ctatactatg tctaaatcaa ctacttactt tttgtaagtt aacaggagga
     8281 aatttatcat ggatacacaa atcagaggaa ttcaccacgt cacagcaatg acatctagcg
     8341 ctacaaaaat ctaccgcttt tttacagata ttttaggaat gcgcttaatc aaaaaaacag
     8401 ttaaccaaga tgacatcgaa acttaccatc tttactttac agacgatact ggtgcgccag
     8461 gaacagatat gactttcttt gattttccaa atcagccaaa aggaaaaaaa ggaacagata
     8521 cgatctctcg tgcttctttc cgtgtgccaa gtgacgcagc attagacttt tggttggaac
     8581 gttttgaaaa atatgacgtt actcattcag caatcaatga acgtttcggc aaaaaatata
     8641 tcgaatttga agatttcgat aatcagtatt atcaattgat ctctgacgaa aacaatcaag
     8701 gtgtagcagc tggcattcct tggaaaaaca gcaatgtacc gacagagttt gcgatcacag
     8761 gacttggacc tgtctttgtc cgtgtcagtc aattagagca tatccgcttg attcttgaaa
     8821 aaatcttagg attcaaagaa acagaccatt caggagattt ctacttattt gaaatcggtg
     8881 aagggggaaa tggtgcaagc atcattattg aacatcggac agacttgcca aaagcaatcg
     8941 aaggatttgg aaatatccat cacttagcat tgcgtgtagc agacaaagaa gcattggaat
     9001 actggattca agtaatcaat aagattgaat tgccaaattc tggtttcgtc gatcgtttct
     9061 atttccaatc tgagtacttc ctagccgctc atcacgtact gtttgaatta gcaactgatg
     9121 gtcctggatt ccttgaagat gaaccagcag aatcagcagg agaaaaacta tctcttcctc
     9181 cattcttaga agaacgtcgt gaagccattg aagcttatgt ccgtcctttc gatacaagtg
     9241 atgcaaataa aaaacgtgaa taatctcttc atgctacttc atttgcatct atagaaacag
     9301 aaaagaggga acaaaatgac ctacattttt gaaaaaggaa caagtggcaa gcatttgatc
     9361 ttacttcatg gcacaggtgg cgatgaacat tctttactgg atatcgctca ctttcttgca
     9421 ccaaacagta cattgctttc ttttcgtggc acggtccaag aagatgggat gaatcgtttt
     9481 ttcaagcgta atggattgaa tcaattcgac ctggacagtt tggaaaaaga aacagataaa
     9541 ctccatgaaa aaatcaaaga aatcagcgaa aaagaactga taccgttaag tgactggctg
     9601 cttgtcggct attcaaatgg cgcaaacatc gctgctcatt tattattgga gcgtgagacc
     9661 gagctaaata aagggttgtt tttccatccg atgtctctag gtgttcatca aagccgttcc
     9721 acattagagg ataaaaccgt ctggatttct tatggtgata acgatccaat cgtttcccct
     9781 gcttccttta aagaattggc cgatcaattc gaaacacgcc aagcaaatgt gactattcag
     9841 ctaactgtaa caggtcatca gttgacgatg gacgaaatca agcaagcgag ggaatggaca
     9901 gaccacctct taactaaata aaagaaaaga actacgactt acataataca acctgaagca
     9961 gatatcgatc tggatcagat tttgtgaaag tcgtagtttt ttgtgtcaga taggttagtc
    10021 tccaccagct gcaataacag atttattata aatagccgtt ttatgtttca actcttccaa
    10081 tcccccgatt ttctcacctt ttgaataaaa accgtcagta atccctaatg cttgaaaaat
    10141 cgtctgcttg taggcaattt ctgcattcca ttcaactgta tcaaggattt gcagtgcttt
    10201 atggatagct tcgattcctg attctccacc tgtgttgatc aatacgccat gagagttgag
    10261 cagcaacata tgcggtaatt cgagatgctg tttttttaca tagtcgcttg tcaattcagc
    10321 aagttcttca gagcaattag gctcaaagtc catgacagga atctctttta ctttagctgt
    10381 ggcttctgtc aaattaggca taggcaaccc agtagtcgcc cagaacattg cattaggcgc
    10441 atgagcatgt aggacagctt tgatagctgg attagtctca tacatcgctt catgcatatt
    10501 gatttcacga gttaaatgac cgactccgtc aacgattcta cgcgttcttg gttctactac
    10561 tagaacctgt gcaggagaaa tctctcctag ataggcttct gacatcatgg tcggcgtcat
    10621 gatgatgtag tctttccctt gatcatcagt ggttttaaag gaaaaattcc ctcctgcgac
    10681 atttgtgttt ttcctttcga aaatcactct tacgatacgt gccatgtcct gtcgttctct
    10741 ctcaaataac atacgtccat ctgacataat gacccctcca ttatcttttt ataaaacttt
    10801 tttctctcaa tatttctttg gtagcttctt ttattttcat tgattcttct attttccgtt
    10861 ttcttttttc atcgtactct ctgaataaaa cgtgatttcc atatttatag atcactagtg
    10921 cgaaaaaaat gaccaataga ttgatgatga tattctgagt aaagagtcca tatagaaata
    10981 gcagaagtgt accacaaaga gcgactgtcc attttatttt atctgtctct tctcttttca
    11041 ttactttccc accatttcaa tgctattttt gtagttcagc ataacgttca ctcggtactt
    11101 tcgcttttat catatattta taaagtagaa caaacagtcc tagaaaaatc acgcatccta
    11161 caatagcgag aatattccta ttaaatgcct ccgcaaataa aaccctgaac tccggccctt
    11221 ctatggatga ccatgtgatc aactgtcctt tcttgattcc ctcgactgct ccgctttttt
    11281 ctgctaaatc actgagtatt ggcgcaaggt aagtggcccc gtacaaataa ataggcatag
    11341 tgatgattcc caagatcagc atacgagcca agtttcctcc tgttaaaagt aatccgccga
    11401 ccgcaatcga atagttgata atccctgcta atggcaatac ttggtttcct ggcaaaataa
    11461 tggcataacc aataaacaca ggaacaagta aaatagcagt aacccaaatt tctgaacgac
    11521 cagctaatat cggccagtcc aatccgatat atacttcacg atccttaaac cgctttttca
    11581 taaattctga catagcgtct gctagaggag acaaagattg catgaacatt ttagagatca
    11641 taggaaacaa acttaatgct gtagctgttt ggatcgctag attaagagaa cctgaaacgc
    11701 catacgcagc agcaaagcca agccccaatc caataatgaa tcccattaca gagttttcag
    11761 agaagatccc aattttcttc ttcaatgcat tcgtatccgc tcgtttattt aaagcaggaa
    11821 ttttatccat gatcatattg aaaggcagca acaagacagc ggcagatgtc ataaaatgag
    11881 tcactgttac taatggaatc cctgtgagat cttgtatatg tttttgaaac atatcaccaa
    11941 gctttaattc caatataact tggaccattg cagtaagaaa gcctgcagcc aaactaccgg
    12001 aaacataata aactaaataa gcagttaaag ctttccccca aacattccac atatcaacat
    12061 ttaacgtctt cgtccaatta aaaagtaaca gtacaaaatt gaccccaatc gttactgcaa
    12121 aaaatataaa agcataactc cacgaccatg tgatgcttgc tgcacctgtc cacccaagat
    12181 caagtgcagg caaactgatt cccgtatttt tagccaatgc ttctgatgct ggacttactg
    12241 cgttagacat atagccaatg accatagaca ttccgctaaa agcaatccct aaagtcaaag
    12301 cagacatgaa tgcttttttt aatttcatcc ctgccaacaa tccaagtatc aaaataatca
    12361 ggggaacaaa aatggctgat cctaagttca aaacataatt gataatggtt tggaaaactt
    12421 ccataaataa accttctttc tttctaaata tagtaaataa aactatgaac caactttttt
    12481 atccaacaat cctcctttac tgtactaact ctttgatact gtcataaata ggttctacac
    12541 ccattccagt taaaaatgga attcctggaa atacttcaat acccaaatct tttgcttgtt
    12601 caagtacttc ctcatcaggt tgtgcgacat acacatagat tccagcagtt agaagctcat
    12661 tttgaatcga tttgtaatca actgcttcta caggaaatgc gatgccgtca tcttctaaca
    12721 tactttggat cttactagcg attgtctgac tcgttgctac tccacttcca catgcgacaa
    12781 ttaaccgatt tttcattttt tcttcctcct aaatataatt tgtaataagt tcgtatatgt
    12841 tttgcggtgt ttcttgtttc ttgtaatttt ccacgaatgt ttcatccatg aataaattca
    12901 taaacgcttg aagtaatcct acttgttctt ttggttcttt gattcctaaa aaaaatagat
    12961 tttctacagt catttcctga ttatccccca tttgataaaa ataaattggg ttcttcagct
    13021 gcataacaaa aacaaatggc ttttcgacat attcacattc cgaatgtggc agcgcgatat
    13081 ttaaatactt agtaatcaat ccagtaggaa attctctttc tcttgcaatc agtccattca
    13141 aataattctc attgacgtac cccatttttt ctaattgttg tgcagcgatt aaaaaagctt
    13201 cttctttatt ttttacttct accgctaatt gaatcaaact aggaacgaac atctctttat
    13261 atttcattcc aacctcctcc tttttgatta agtttgttaa tttattgttc tactaagttc
    13321 atctttgttt tacaagaaca ttatatttct gttagtttgt tttgtcaacg ctttcaaaag
    13381 aaatttattt tgttaaattt gatctacttg aaaataaaat cgtttatcta tataattaag
    13441 aagaacaaag tcgaacaaaa attgactttt atgttaaagg agtaattcat atgctgaaaa
    13501 aagaaagaca agaacaaatc attacattat tagagaaaga tttttattta actaattcag
    13561 aaatcgctaa caaattaggc gtatcagaaa tgaccatacg ccgagatgtc actgaattag
    13621 caaaaaaaaa taaagtaaaa aagctatatg gtggcgttga acgaaaggaa ccttataatc
    13681 gagaactatc gactcaagaa aagattgata cgaatgtagc cgaaaaaaaa tatattggtc
    13741 aagtcatgaa cagtatgatt cccgataatt ctactattta tgtcggtgct ggaacaacga
    13801 tcctatatgc acttcctttc attcaaaaga aaaatctatt tgtaattaca aacagtttga
    13861 ttgctttcaa ctatttaaag aatcatacaa gttatcggat tttacttact ggtggcgaat
    13921 tttctcctat tacagaagag ttcattgggg aagttgcaga aagatctttt gaaagtttga
    13981 atgtcgatat ctctttcgct gcaacaaacg gcatatttga taacaatgtg acaaccgctc
    14041 aatttattga gggaaatatt cagaacgcag cgctcaatgc agcaaaaatc aaatgcattg
    14101 ttgcagatta ttcaaaatta aataaatcag acgtctatac ttttagaaaa ttaaccgatt
    14161 ttgattatct aataaccgat aatcatttat cagaacagga tttcaatcat tattcaaaat
    14221 acttaacaat attaaaggag cccaaaaatg atactgacaa tcacgatgaa cccatccatt
    14281 gattccattt attttacaaa acactttgaa ttaggtacga tgacacgctt tgattctccg
    14341 gtacgctatg taggcggcaa gggaatcaat tgcggacgag cttctgctct actaggttca
    14401 tcagttattt taacagggtt tttgggcggt tattttggca gtatgattca agatatgctg
    14461 aaaaatgaaa atttgtttga tttgcatttt ttacctattg cagaagaatc aagaggtgcc
    14521 gtcacgatca tgcatgatga tgaaacgcag acagagctag tggaagctgg tcctgaaata
    14581 gcagatacta ctatcaaaaa attaatgaat gatatcaaac taatcagtaa aaataattac
    14641 ctttcagtca ttacaataaa tggttctgtt aacaatccag atcccttttt gtatgctaaa
    14701 ttattacaat atatcaaaga acaaatagat ccttccacct atatattaat ggatatatct
    14761 ggacaacatc tccgtgaagt cttagcaagt aagtataaac cgatgttcat caaacctaat
    14821 atccatgagt tttcagatat catccatcaa aaaattactc aaaaaagcga cataaaacac
    14881 catctaaaaa caaatccact atttaaagat atcgatgtta tcatgatttc ttgcggctcg
    14941 tatggtgcag tagtcaaagt aaaagaagaa ttttggaatg ttgagatccc taagattcct
    15001 atcattaata caacaggctc tggggatgcc actgttggag gttttgctca ttcattagaa
    15061 cagaattatc ctttaaaaga atgtgtaagg tacgcaatgg cttgcggcat gtcgaatgca
    15121 cagcaatcag aagtcgggaa agttattcca gctgatgtaa aaaatttagc aaaaagcatt
    15181 aaaatcacag aaatgaaata aatagtacaa agtaaataat tgtttatatg aaatgagaca
    15241 caaagagact gtgacaaaaa acgtgtgtac cgacccccaa aagttagatt ttttggtcta
    15301 acttttggga gtcgatacat tgtcacagtc cttttttcga ataaacggtg ttctcctgcc
    15361 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctatttt ttgaaattgc
    15421 tccttgtgac tcagagttaa acggcaggct cacgacctct tggtataaac ggtgttcaaa
    15481 agcttgcatc tacgctaatg agtccaacca agtcaaaaat gaggagcttt tttggctgtc
    15541 cttattactt gatgcagggc agttttttca taatctcttt tagattaaat ccttttggtt
    15601 acacaaggtt ctcttcaaag aatgttgcca ttttttcaaa tggaatgatg tctgtctggt
    15661 cgtatagatc tgtatgggat gctcctggaa tgatcatgag ttccttgttg tcacctttta
    15721 gttctttgaa agcgtcctcc ccaaagtaac gtgaatgagc tttttccccg tgaatgacta
    15781 agactgcatg acggatttct ggaatataat gcaatagttt tgcattcata agtgacaacc
    15841 ctgatgtcct attccaacca tcattggagt ttaaagaacg gacatgatat ccgcggtctg
    15901 ttttataata cgcatgatag tctttgacaa attgaggagc atcttcaggc aacggatcaa
    15961 ccacacctcc agcacgtgcg tagtcccctt gtttgaaatc aatgatgcgt tgtgcattta
    16021 attcttcacg catttcatat cgggcatttt catccataac atcgaagtag ccatttgctg
    16081 ttactcgagt catgtcatac attgtggaaa caactgttgc tttgacccgc gtatcgatag
    16141 ctgctgcatt caatgccata cctccccatc cacaaatacc aatgattccg attctctcag
    16201 ggtttacatt tttttgtaca gataaaaagt ctactgccgc ttggaaatct tccgtattga
    16261 tatcaggcga agcaacatat cgaggtactc ctccgctttc tcctgtaaac gatggatcaa
    16321 acgcaatcgt taaaaagcct ctttcagcca tcgtctgcgc atatagacca gacgattgtt
    16381 ccttcactgc accaaatggc ccgctcacag cgattgccgg caatttcttt tcacttactt
    16441 cttttggttc atataaatca gcgactaatg tgataccaaa acgattcggg aagctcacct
    16501 ttcgatgatt gacttgattg ctttttggaa atactttgtc ccattcttgt gtgatgttta
    16561 attctttatt catattctag actctccttt gatcaaaatt gacatttgtt tttgaatgta
    16621 ttattatgat aaaacttaaa cttgacttta ggtcaaggta aaaattttat tttaggagga
    16681 tttttttatg tattccattg gacaagtgtc tgaaatgttt ggattgcctg tttctacttt
    16741 acgttattac gacaaagagg gattatttcc tgatatccag cgtgtttctg gtattagaaa
    16801 attttcagag aatgagttag aggctttaag agtcattgac tgtttgaaaa aatctggttt
    16861 agaaataaaa gacattaagc aattcatgga atggagcaaa gaaggcagta agacatttga
    16921 ggttcgtaaa gaactatttg aaaaacaaaa agaagtagta gaaaaagaaa tagcaaaatt
    16981 agaaagagta ctcgctatgc taaactacaa aagctggtac tatgaagaag caataaaagc
    17041 cggcaatgaa gaagccgtat tgacaatgat tcctgatgat ttaccccaac atgtaaaaga
    17101 agcttatgta cattctcatt gaacaaacgg ataactcact ctccttctct atgaacttca
    17161 tgaaaacatg aataaacacc tctaactgat tgttagaagt gtttattttt tcactcaagt
    17221 atcctgcact tacaaaaata tcctttttac cgccatccta attctggagc gatatctttt
    17281 acgattgttt caagcaagtg catgttatat tctaccccca aagtattagg aatcgtgaca
    17341 agtactgtgt ctgcttcttt gattgcttca tcttcagcta gctccttgat taattgatca
    17401 ggttcaccag caaaagtttt tccaaaaatc gttgggtttc ggtgataagc aagatagccg
    17461 acagcatctt gttgcggact accgtcttgt ccaaataagc gtcgatccaa atcagtggtg
    17521 attggctgaa tcgagcgagt aactaatgtt cttggttcaa aatcatgacc tgcttgcttc
    17581 cacgcttctt tatacgcacg taattggttt gcttgttgga tatggaatgg ctcgtgggat
    17641 tcgtaattga ctaatgtaga tgattgcaga ttcattccat gttgtgctgc ccagattgcc
    17701 gtttgctgcg aaccagctcc ccaccagatt ctttgtcgta atccttctga ataaggttct
    17761 atccgtaatt ttcctgatcc ttgtgggaac attggttgag gatttggttc ttcaaatggt
    17821 tttccttcaa gtaattccaa gaattcaagg gtgcgttccc gtgttacctc tttgatttct
    17881 tcttcactgt aatcataacc aaagtatttc cagccatcta gaacttgttc aggtgaacca
    17941 cgccccactc ctagttgcac tcttccttgc gtgatgatat ctgtcaatcc ggcattttct
    18001 gccatataat aagggttttc atatcgcata tcaataagtc ctgtaccgat ttcaatctta
    18061 cttgtctttg cgccgattgc cgctagcaaa ggaaacggcg acccaatctg aggggcaaaa
    18121 tgatgtacgc ggaaaaacgc tccgtccact ccgatttttt ctgcttccac cgctaaatca
    18181 atcgattgca aataagcatc ttgagctgat ttcacaagcg atccttgctc catccaatgt
    18241 ccaaagctta aaaagccgat ttttttcaag gtaatgacct cctttaaacc tacaaaaatg
    18301 gaaggttttt tatttttaat atgaactgat tctactactt tttttcttaa aagacgaata
    18361 attgttctcg cctcacaatc aagaaatgta tcggcttact acacccctac tttcttcatc
    18421 atctgccgtg tcttaatctc ttttaacagt ctgttacatc gttgattttt gactcaaaat
    18481 gattgtttct aacatgtatt tatggtaaaa aagacaagat tagttctttg tacacatcga
    18541 tttatacgct gaacaaaaag aaatatataa actaagaaac ccattgtttt ttcgctccat
    18601 cacatagagt ttactttaga taaacagtga catttactaa tccataacgg aggaaatcaa
    18661 atgaaaaaaa tctatttctt atgtacgggc aattcatgcc gcagtcaaat agcagaaggc
    18721 tacgcgcgta aatacttgtc tccttcaatg tttgagataa gaagtgcggg gatcgagaca
    18781 catggcttga atccaagagc tataaaaatc atggcagaag acggcatcga tatttccaaa
    18841 cagacgtctg atttgattga tagagattac tttactcagg cagacttgat cattacactt
    18901 tgtggtgatg ccaaagacaa gtgccctgcc gttccaaaca atcacgaaca ccttcactgg
    18961 gacctaaaag atcctgcaca agctgtcggg agcgaagaag aaatattaac ggaattcaga
    19021 aaagtcagag aaggaatcaa aagaaaagtt ttagacttaa atcaacgtac atcgatcgta
    19081 taaagaagta ttcaaaatga tttttcaagc actaagaaac aatgttaaaa aaatagcttt
    19141 ccatatttta caaaattgtg ccttattgcc gacaaattgt ctcttgaaca ccgtttacca
    19201 gtagtcaaga catcgtaagc aactataaaa aattataaga aactcaaaat aaaaggatgt
    19261 gccacaactt tggccacatc ctcttttttt gtatttagtt ttctacttcc aaataaatag
    19321 atagaattat tataaatata aaggcagttt tttaatcgct acttattcat aactagataa
    19381 caacgttaag atttctagcg gatgattgga ttgcatcaac tgagccattt gttctggctg
    19441 cttttctaga tagtgaataa cagaaaatat ttttttgaac tctgcatgtt tcaaatcatt
    19501 tttctttatt gctaatagca gtacgatacg tatctgttcc ccgtgccata ggactggttc
    19561 tttcattgta gcaatagaaa tcatcgaatg cttcacatac agcggattac cgtgagggat
    19621 tgttacaaat ggtgctactc tagtactgga gtgactgtct ctttccaaga cagaatcgat
    19681 gaattccggc tccacataat gatgttccac taagacgttt cccataaagt ttaaaatatc
    19741 ggctgggtct gtccgttcaa gttgaggaaa gatcagaaat ggatgagtaa agcttgctag
    19801 atcaaatgtt tttttcttct tgataggcac atgctccagc atatattttg tcaaataggc
    19861 tagatcagtt tctgtgatca ttggtgagat ctcgattact ggaatgctta tatgtttaaa
    19921 tggtaacgta gccaagataa aatcaacaga gggaaaatct tgtctcttta attctggctt
    19981 atattcaact agatccataa tcgttaattc tggaagttct ctgttcagcc gtgcttcgag
    20041 aaaagtagcc atagcttttg aatattcact aactaaaacc gtttgatatc ttctactttt
    20101 ctgatgtttg tgtcgctcga tcgctgcttg gaaatggatc gttaaataag ctgtctcctc
    20161 ttgtggaaca taaaaatttt ccttttcaaa ataatcttct aaaattaact gtaaaactaa
    20221 gaaaagttgt gtgtacgtac taaacacatt tttcgtcaat ggattagaaa tctgcaaacc
    20281 ataatggagt cgaaaataag ttgaagataa atgagacctt aaattttgtt ttagtagctg
    20341 atcttctttt aatgaatagc ctagaaactg ttccacttgt tcgatcaaat gatcagcaag
    20401 tgcgctagat tccttttgaa aacgtgtggc tgcttgtgaa tttgtaaaaa gattggcgat
    20461 acgcaaagcc agataattga tttcatcagg agaaaattca attggataga tggtggctag
    20521 ttttgctaat atttgactac ttaacttttg tgcttgggtg tgttccacga cttcgttttc
    20581 gtctttactt aatttcactt tctggtactg tttcattcgc tcgagcataa agtagatgtg
    20641 aatctccaca ctagccagtc cattcggctg ttctaaatgg ttttcataaa aaacatcttg
    20701 taatgtatgt tggataactt ttaatgtatc tttagcgaag aattctttca acgacggact
    20761 ttgtggatct tctttctgca tatttcgtaa tgtcttaaca agcaactgtc ttttttcttg
    20821 ttcatcgcct ttaagaaaag ttcctagttt tgttttggat actagctgta aatggtgata
    20881 ctcaaaaaag ctttgcaact ctgtcaattc tttacgaatc gtacgttttc caacatatag
    20941 tttttctgaa agttcttcaa gactaaatcc gtcttttgct actagcagat ggaaaatcaa
    21001 taactgacga agctgttttt tatccatttc tgtttgagtc tcttcattaa gcgctcgttt
    21061 cagttgttcc ctttcgtgag gcagaccatt caacaagata cctgtacctt tttttctttc
    21121 aaacattaaa ggcagatgtt tgtctctcaa ccaagtttca atggtattca aatcgctatg
    21181 aatcgttctt tctgacacat ggaaatgctc tgctagcttt tttgttgtta tataattatt
    21241 gttctcactt aattgataga tcaaagattg ctgacgctga ttcataatgt gcagaaactc
    21301 ccttctaaat aaaaaatcga tcatgataac acatctatta tgtctcatca cggtcgattt
    21361 ttcatcttat gttacatttc gcttaataat tggtgaactg ttgttttatc tggcgcttcc
    21421 atcagtgctt gacggaagtc atcatccatc aatttacgtg atagacgttg cagtattttc
    21481 aagtgcatat caccttggct gttttcaggt acagcaatca taaagatcat atgtgcaggt
    21541 tttccatcta aagaatgcca atctacccca gattgacttc tggcaaatgc cactgttggt
    21601 ttaagaacat aagctgattt tccatgtgga atagcaatcc ccataccgat tcccgttgta
    21661 ctttcatgtt ctctatcata gatgacttct ttaaatctgg ttttatctgt gatcgccgct
    21721 gttttttcta aacgttggac taaatcttcg attactccgt cttttgttgt tgctgtttca
    21781 cctaaaacga ttgcttcatc tgacataata tccgtgatag aatcaaaagt ttcagttttc
    21841 gtataaacat cacttgtttc agtctctgat agatctgcta ctgtcacacc atttgctgca
    21901 gcaaccgcag tttgttgtaa tgtcggttta aggattctta acattagcac ggttacagca
    21961 ataccagcaa tcactgctac aaaaaacatg gctacgtgat ccactgctcc taaaaccgct
    22021 acgatcggac cgccatgagc tacatgatct gtcactcctg aaagcatcgc gatcacactt
    22081 cctgtcattg ccccgattac aatgcttgga atcacccgta gtggatctgt tgaagcaaac
    22141 ggaatagcgc cttctgtaat cccaaacagt cccattgcta aggacgcctt accagcttct
    22201 cgttctgttt tcgtaaattt ggtttttaaa atcaatgatg ccagccccaa tgctaatgga
    22261 gggatacaga tagctacagc aattggcccc ataatggaat aattgccttc tgcaatcaag
    22321 ccagatccga acaaaaaggc tactttattg aacggccccc ccatatcaaa agcaatcatc
    22381 gctcctaaga tcatagctag aacaatttca cttcctcctt gcatccctgc taaaaagtga
    22441 gtcaatgaac taaatagaag agctactggt tctccaataa catagataaa cacaaaacca
    22501 actgccaatg atcctaaaat tgggataaca ataattggca tgatcgattg taaagctttc
    22561 ggtactcgca ctttcttgat tcctaaagca atgaatcctg ctaaaaatcc agtgatgatc
    22621 ccgcctaaga atcccgcatt cgcatcacta ccatataatg ctccgtttgc cgctaacatc
    22681 ccaccaacaa atccaggaac aagtcctgga cgatctgcaa tactttgcgc aataaatgca
    22741 gatagaattg gaaccatcaa ccccatagcg atacttccga tactattcat cgttgcccaa
    22801 atcgttcctt ctggtatctt gatcccttca ggtgtagctg ttcctcctaa tgttaaagaa
    22861 atcgcgatca ataaccctcc tgtaactacg aaaggtacca tgtacgaaac gccattcatc
    22921 aaactcttat agattttttt acctaaattt tctgattcac ttttattttc ggaagaaata
    22981 attgcctccg tacttttata gactggcgct tttcctgcta aaatatctct caccaactca
    23041 tctgcatggt ggattccttc ttgcacacct acggttatca aaggtttacc gccgaatcgt
    23101 gatttatcta ttttagtatc tgctgcgatg attacaccag ctgcttcttc gatatctctt
    23161 gcagtcaatt cattttcaat accgatggaa ccatgtgttt cgacttttac ctcggcgcca
    23221 actgctttcc ccgctttctc taagttttct gctgccatgt atgtatgggc aataccaact
    23281 ggacaggatg tgattgcaat aatttttttc attttcctac cgcctttcta catttatagt
    23341 atataaaaac ggtttctttt tattaagcac aaaatgattc cgtaataact gaaattcttg
    23401 gaataaatag aaatttcgct aaaaaaattg atatacgatt tattatgttg aataaaataa
    23461 tattggaaaa tcttacattt ctgtacttct gcttacgtac atctaaatta ttatctacat
    23521 taacaatttc cgtaattaat tataaaaagc ttattccatt taatttcatt ctggaataag
    23581 cttcttattt tctaatgagt tagtgaataa ttttttttac catgcttaat agtatcttta
    23641 cgtctcctat acgcgtatct cctgtttctt tatctataat tgaactataa acatgaggaa
    23701 tcactttttt tacacctgca tcgattgcaa ttttcagaat ttcttcaaag tttgaaagat
    23761 caattcctcc tgttggctct aaatagaatt catattttgc gcaagcttcc gcaactgctt
    23821 taaattcttc cctatgtgct aagcctttca tcgggaaata cttgattgaa gtacctccca
    23881 tgtcttgcag catttgaatg gcagtttcaa taggcacttc agctgctggc gcttgtgaac
    23941 ttaacggacc tgtagcaata ttgacatagc cgatcttacc tgttggtgaa accaatccat
    24001 tgatgactgt atcattttgg cccaataaag cacgagaagt ccccacgcca gtaaatactt
    24061 gattgacatg ttgtggttgt aaaaccccag ataaacgcga aaccatctga ctttggtttg
    24121 gatctccagc gcctaatcca actgacaacg cattattggt tgcgtcttgg taccttttca
    24181 tatcttcgat tgctgcttca tcgctagcat aatttttcga tagcacacct agtacgacat
    24241 gcccttccgc tgcttcatag catgcttttg cattttcaac tgaatgagct aatacattta
    24301 aacatacacg atttttataa taatttggga taactgacaa tttaaaagcc tcctacatga
    24361 tttctctcaa tctttttata attttttcca tttcgttttg gctgacggaa cgaatgtcga
    24421 actcaatgat tccgttattt gcttgatatt ctcgggtgta gactgctgga tttcgtgctt
    24481 ttaattcctg aatgacttct ttcgcacttt tatcgccatt aatcgttaca cttgctcgat
    24541 aaatatctct gccggcacca tcttgaacta ttttcgctga aaggtcagga agttgattga
    24601 ttgcgttaac aaaaggcgct aatcgttgct tcatggagtc accggattcg ctcccatttt
    24661 ttaaataatc ttctagtgcc tgggtaaagc ctaaaatatt atctttaccg attttcattg
    24721 ctcggccaat acctttagac tgtagccgaa tccattgaat atacttcttt tttcctatga
    24781 ccagtccggc actcggacct tcgattgctt ttgccccaga gtaaatcact aagtcaacgc
    24841 ccatttcgct atatttgaat aaatcttctt ccgcagctgc atcaacgatc aatggcaaac
    24901 gatgagcttt ggcaacttct accatttctg ccaccgtcag catacttttc tgaactgcat
    24961 gatgactttt aatatataga agcgcagcag ttttttccgt aatcatcatg gaaacatgtt
    25021 ccggtgtaca catatttgca tagccagctt ctatgacttt tcctccacct tgagcaacca
    25081 ttacttcaac cggtgtccca taatctacat tatgtccctt aggtatgatg atttcccgtc
    25141 tagcgatttt ttctgtatag ggatgataga catggtacat gtcgccctgt ccgattaaag
    25201 cagcaacaga ttgagcaatc cctgctgaag cagaagaaac gatttgcgca tcttccactt
    25261 tcagtaattg agaaagataa gcgccggttt tgatggcaag atcactcatc tcaaaaaaat
    25321 gctcgcctcc aaatcgttgt gctgctagga cactttcaga taccttagaa acccctagga
    25381 tcgtcatttt tcccgatgca ttaatgactt cttttaaatc aaactttcca tagctaattg
    25441 tcatatatga cgcctccaat aattgtttta atcggtaaaa tttgttcttt agctatacgt
    25501 gtaaatccgt tggaatcttt caattccttt tcattttcaa caatctcaaa tatcgtcaaa
    25561 tctgcatcat aattgacagc taactggccc ttattcttca aatgaaaatt ccgtgcagga
    25621 actgctgtaa ctttttcaat gatttcttcc caagaatagc cgactaccct caatttttct
    25681 aaagtagttg ctagatcaaa aactgggcca tttgtacgat tgcggatgta gatatccgta
    25741 ctaatagagg tagctttcat tcctgctttt agtgcggttt ctgccacatg gaaattaaag
    25801 ctatccgtcc catgtccaat atcaaagacg acaccattat tgtaagcatc ccagacaaag
    25861 tttttaattt gatctgtctt tggatctaaa ataccgtttg gttttccatt aaaacaatgg
    25921 gtcaatacat cgcctctttc cattcttgac aatacttctt ctaactcagg aggtgcagaa
    25981 ccgatatgaa ccatcaatgg cagatcattg ttttctgtct ggattttttt agctaattct
    26041 aatggcttaa tgccattttc accaacaact gtcttactca ttctcgcttt aatcccgaca
    26101 ataaattccg gcagttctgc taacgcagta cttactagat cttcttgaat ctttgttaga
    26161 tcagcaagtt catcttgttt gacaatgccc catttagaga tattcattag cgcatacaca
    26221 tttgtcttga ccgatttcgt caattgataa aattcacgga tgttttctgc tcccgtagtt
    26281 ccagcatcaa tgaccgtcgt cacacctttt tcgaccccta tctgatcagg atagtcataa
    26341 taaagattca tcttttcata gcagtggaca tgatcatcga tccacccagc ggataaataa
    26401 tactttccct ccaagtcaac tacttcttgc gccggctgat ttatttctgc tgcaactgct
    26461 ttaatcatgc ctttttctat agcgacctct attttagatc catcaactaa ccttccattt
    26521 ttaatcaaag tatcaaacat ttaatgaccc tctttctatt aatttactgc cggtaagatt
    26581 gctcctaaaa tcattgctcc taaaatagca cctccagcaa ttggtttttt ccaagcatag
    26641 aatcctagtg ctcctaatgt agcgccaatg ccgattggaa tagatgctgc acacgcgctt
    26701 aatataatta atggacccaa aaaacgtcca gatgaatttc ctgcccccat catgacgtca
    26761 gcaccaaaag tggaatttga ttgattgatc gtaaatttac gaatcaagac aatgatgcca
    26821 ccaacaatca aacctagaac caaaccagtc aataaagaga gtgcaaaatt atcgattggt
    26881 gccgctattt tcatacttaa gagaattgct ggtaccccaa ttccgatccc agttaggatc
    26941 gaaccgccta agtccaagat cccaactaaa gagccttcta aaattctggc aaataagaaa
    27001 ctagcaccaa acgctgccgc tgcgccatag gatccgccat ccagcccggc ttttaacatc
    27061 gccacgatcg aaacttcatt gaatgcaccc acaccataca tcacatacat gtgtgttcct
    27121 gcaaatacag cagcggacat aattcctacg attagcggaa aagaccagtc tgcaaaccaa
    27181 aagctctttt gccgctttgc ttctaatact tttgcttgtt cttctgttgt ctccatgatt
    27241 ctcctcctat ttcacactaa agatgttgtg caagttatca agccaattag gcacttgaag
    27301 ctggaagctc ttgatcattt ccatatcaaa tccacggaaa aatccactga aaataaataa
    27361 taaaacaata gcgatcatca tgctcttcgt gacgcgattc caaccgctct catcgacgcc
    27421 tttaccaatc aagattccta gaacaacacc aggtaccgca tttcccataa tcaattgcgc
    27481 gataccacca aaaagagtgc cccaaaaacc agttcgtttc cctgcgtcta aagctgccaa
    27541 ccaaaaaata actggcatta ctgtattgat cagaatcgtc gctgctggta ccaaaacgga
    27601 aaccgccgtc acttgtaacg cctctggaat tgctgcagct gttgtgttta agaaggctac
    27661 caccactgcc ccgataaccg ctccaatgat ccccattttt ttcggatcat gcatggtctg
    27721 ggtgacatct ttgttttttg ctagaagtgc tgccgctgcc caattgggaa tgattcgatg
    27781 cgtcacatct tgtgtgaatg caccagcacc aactgaagaa gcccatgcat taaagaaaaa
    27841 gcctaaacca aatgaaaaat gagaagctgg atcacccatg caggcattca tttcgcctaa
    27901 tgttctaaaa gctcctaaac cttgtgtatt aggtgcatga aacattcgag ctgccccgac
    27961 agatgctgca aaaccaagta atcccccgat aatgaccgat ttgactagaa tgattacgaa
    28021 atccacgtta ttctttcccc cttatgattc aaaattagat ttttttagtt acaaatggaa
    28081 tggcaatccc atttggatct tcagtcattt gctggctgaa cgatatgtct gccaatttta
    28141 tttctgttac agccacagta acagagagtt cgacttcata gctaatacgt tgccgtggga
    28201 agaagaaaaa tagaaagcgt tctgtgtatt tgttttcgac cgcctttaaa atatccacat
    28261 tgactggttc gattcgcaat aatacgtttg attgttccat tattttgttt tgaatttctg
    28321 ctaacgctga tgcaaaagca tgttgctttg tttccccttt accggagacg ataacttttt
    28381 gctgaattgt cttttgaacg ctcatttcat gtccctcttt ctattttcca tgctttttga
    28441 taaattcttc tgtcagacgt ttccctaatt cctcttgatc cataaagccg aaacctagaa
    28501 cagttttgcc atccttgata gctgtgatcc cttcttcaat cgaacgcata ccatgtcgtt
    28561 ctggatagcc atattttgtt gctgctgtta atgcacctgc accaccacta ccacaaaaag
    28621 agatacccat atctgcatgc tcttgttgca tcacatcgcc caaacgcata tctgccccca
    28681 ttcctggtac gacaactgct tttccaccag ctgcttctac ccctttggcc acattttgtc
    28741 ctttacccat acggtctgca ataactactt taatcatgat
//
LOCUS       DACEOM010000037        28627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEOM010000037 DACEOM010000000
VERSION     DACEOM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.038"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..586)
                     /locus_tag="IW781_001535"
     CDS             complement(5..586)
                     /locus_tag="IW781_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1182536.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IW781_001536"
     CDS             complement(769..1395)
                     /locus_tag="IW781_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1182537.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IW781_001537"
     CDS             complement(1417..2748)
                     /locus_tag="IW781_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1182538.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IW781_001538"
     CDS             complement(2903..3304)
                     /locus_tag="IW781_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1182539.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IW781_001539"
     CDS             complement(3390..3596)
                     /locus_tag="IW781_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182540.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IW781_001540"
     CDS             complement(3691..5193)
                     /locus_tag="IW781_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1182541.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW781_001541"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW781_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1182542.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IW781_001542"
     CDS             complement(5764..6381)
                     /locus_tag="IW781_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182543.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IW781_001543"
     CDS             6602..7975
                     /locus_tag="IW781_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1182544.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IW781_001544"
     CDS             8050..9162
                     /locus_tag="IW781_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1182545.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IW781_001545"
     CDS             complement(9234..10871)
                     /locus_tag="IW781_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1182546.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IW781_001546"
     CDS             complement(11039..11647)
                     /locus_tag="IW781_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1182547.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW781_001547"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW781_001547"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1182548.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IW781_001548"
     CDS             complement(13201..13953)
                     /locus_tag="IW781_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182549.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IW781_001549"
     CDS             complement(14094..15044)
                     /locus_tag="IW781_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1182550.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IW781_001550"
     CDS             complement(15045..15884)
                     /locus_tag="IW781_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1182551.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IW781_001551"
     CDS             complement(15881..16627)
                     /locus_tag="IW781_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182552.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IW781_001552"
     CDS             16865..18235
                     /locus_tag="IW781_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1182553.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IW781_001553"
     CDS             complement(18290..19186)
                     /locus_tag="IW781_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1182554.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IW781_001554"
     CDS             complement(19467..20144)
                     /locus_tag="IW781_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1182555.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IW781_001555"
     CDS             complement(20235..21140)
                     /locus_tag="IW781_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1182556.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IW781_001556"
     CDS             21315..22769
                     /locus_tag="IW781_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1182557.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="IW781_001557"
     CDS             22785..23816
                     /locus_tag="IW781_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1182558.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IW781_001558"
     CDS             23823..25010
                     /locus_tag="IW781_001558"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1182559.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IW781_001559"
     CDS             25166..26548
                     /locus_tag="IW781_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1182560.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26675..26905
                     /locus_tag="IW781_001560"
     CDS             26675..26905
                     /locus_tag="IW781_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182561.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27105..27773)
                     /locus_tag="IW781_001561"
     CDS             complement(27105..27773)
                     /locus_tag="IW781_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1182562.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(27962..>28627)
                     /locus_tag="IW781_001562"
     CDS             complement(27962..>28627)
                     /locus_tag="IW781_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1182563.1"
                     /translation="NKKKVQRLIKKLGIKVTAYTRKSRRYNSYRGKVGAVAKNRIHRR
                     FYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMDMFNSEILSYRISEKPNAL
                     AVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIFQSMSRKGNCLDNS
                     LMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTV
                     TVV"
BASE COUNT         7962 a         5849 c         5257 g         9559 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtaacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaatgaaga
    26641 agaagcacgc aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat
    26701 actgacagaa gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga
    26761 atttggaaga ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta
    26821 ctttatgatt gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga
    26881 aacaaaaaaa tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata
    26941 agaaaggatt tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga
    27001 agaagcaact tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt
    27061 tttgagtaac aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg
    27121 taagattcat gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat
    27181 tcaacataaa tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc
    27241 catgcttcag tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca
    27301 agcaggatcg ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca
    27361 ttctctagga taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt
    27421 gctcgcaact gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg
    27481 tctttgatta gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg
    27541 acgatacgtt cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc
    27601 cctacatgga cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta
    27661 tagactaaat caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact
    27721 aataataaac cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct
    27781 ccccctaact atctgttaat taaattatat atgctttctc acgaacacgc atggaatata
    27841 caaggaagaa atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa
    27901 agttagactt ttttggagtg gagaaatcca ctcctttttt ttatgctcta aaatgatatt
    27961 tttacacaac cgttacggtt gttttacgaa attggacgag actttgccag tttaatttct
    28021 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
    28081 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
    28141 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
    28201 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
    28261 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
    28321 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
    28381 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
    28441 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
    28501 gatggatccg atttttggct actgctccaa cttttcctcg ataagaattg tagcgacgtg
    28561 atttacgcgt gtatgccgtt acttttattc ctagcttctt gatcaagcgc tgaactttct
    28621 ttttatt
//
LOCUS       DACEOM010000038        28546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEOM010000038 DACEOM010000000
VERSION     DACEOM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.039"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            227..715
                     /gene="purE"
                     /locus_tag="IW781_001563"
     CDS             227..715
                     /gene="purE"
                     /locus_tag="IW781_001563"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1182564.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            699..1832
                     /gene="purK"
                     /locus_tag="IW781_001564"
     CDS             699..1832
                     /gene="purK"
                     /locus_tag="IW781_001564"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1182565.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1845..3140
                     /gene="purB"
                     /locus_tag="IW781_001565"
     CDS             1845..3140
                     /gene="purB"
                     /locus_tag="IW781_001565"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1182566.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3320..3697)
                     /locus_tag="IW781_001566"
     CDS             complement(3320..3697)
                     /locus_tag="IW781_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182567.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            3953..4681
                     /locus_tag="IW781_001567"
     CDS             3953..4681
                     /locus_tag="IW781_001567"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1182568.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            4681..4935
                     /gene="purS"
                     /locus_tag="IW781_001568"
     CDS             4681..4935
                     /gene="purS"
                     /locus_tag="IW781_001568"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1182569.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            4937..5608
                     /gene="purQ"
                     /locus_tag="IW781_001569"
     CDS             4937..5608
                     /gene="purQ"
                     /locus_tag="IW781_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1182570.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            5609..7831
                     /gene="purL"
                     /locus_tag="IW781_001570"
     CDS             5609..7831
                     /gene="purL"
                     /locus_tag="IW781_001570"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1182571.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            7816..9255
                     /locus_tag="IW781_001571"
     CDS             7816..9255
                     /locus_tag="IW781_001571"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1182572.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            9287..10330
                     /locus_tag="IW781_001572"
     CDS             9287..10330
                     /locus_tag="IW781_001572"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1182573.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            10327..10905
                     /gene="purN"
                     /locus_tag="IW781_001573"
     CDS             10327..10905
                     /gene="purN"
                     /locus_tag="IW781_001573"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1182574.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            10902..12437
                     /gene="purH"
                     /locus_tag="IW781_001574"
     CDS             10902..12437
                     /gene="purH"
                     /locus_tag="IW781_001574"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1182575.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            12453..13727
                     /gene="purD"
                     /locus_tag="IW781_001575"
     CDS             12453..13727
                     /gene="purD"
                     /locus_tag="IW781_001575"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1182576.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            13849..14763
                     /locus_tag="IW781_001576"
     CDS             13849..14763
                     /locus_tag="IW781_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1182577.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(14896..16461)
                     /locus_tag="IW781_001577"
     CDS             complement(14896..16461)
                     /locus_tag="IW781_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1182578.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(16454..17905)
                     /locus_tag="IW781_001578"
     CDS             complement(16454..17905)
                     /locus_tag="IW781_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1182579.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(17919..18935)
                     /locus_tag="IW781_001579"
     CDS             complement(17919..18935)
                     /locus_tag="IW781_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1182580.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            19155..20243
                     /locus_tag="IW781_001580"
     CDS             19155..20243
                     /locus_tag="IW781_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1182581.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            20297..21823
                     /locus_tag="IW781_001581"
     CDS             20297..21823
                     /locus_tag="IW781_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182582.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            21825..23030
                     /locus_tag="IW781_001582"
     CDS             21825..23030
                     /locus_tag="IW781_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1182583.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            23287..24264
                     /gene="guaC"
                     /locus_tag="IW781_001583"
     CDS             23287..24264
                     /gene="guaC"
                     /locus_tag="IW781_001583"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1182584.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            24528..25952
                     /locus_tag="IW781_001584"
     CDS             24528..25952
                     /locus_tag="IW781_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1182585.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            26413..27522
                     /gene="pdhA"
                     /locus_tag="IW781_001585"
     CDS             26413..27522
                     /gene="pdhA"
                     /locus_tag="IW781_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1182586.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            27525..28502
                     /locus_tag="IW781_001586"
     CDS             27525..28502
                     /locus_tag="IW781_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1182587.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9221 a         4879 c         6447 g         7999 t
ORIGIN      
        1 agcttctcat gtttgttgaa tattgttcct tgtgaccgag gagttggcag gagaacaccg
       61 tttatccata aaaaggaatg tgacctaact tttgtcacat tccttttttt tatctttaat
      121 aaacacgaac aataaaatgt ttttctttgt taacattcga aatttctttg acatcaaaaa
      181 aagtgattgt taaaatatga atgtagaaaa actgggagga aaaacaatga cagcaaaaat
      241 ctctgtgatt atggggagta cttcagattg ggaaacaatg aaagaagcat gtcaaatatt
      301 ggatgaattc ggtgtgtctt atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt
      361 gatgtttgaa tatgcacaaa acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc
      421 agggggagcc gcgcatctgc cgggtatggt cgcatcaaaa acaactttgc cggttatcgg
      481 tgtaccggtc caatcacgaa cattaaatgg tttggattcg cttctttcga ttgtccaaat
      541 gcctggagga gtacctgtag ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg
      601 actacttgcg atacagatgc tttccatgta tgatttagaa ttggaggcta aattggctga
      661 aagacgttca atgttggcta aaactgttat agaaagtagt gatcaacttg gttaaacctt
      721 tgttgcctgg ttcaacgatc gggatcgtcg gtggcggtca attaggtcgt atgatggcga
      781 ttagtgcaaa agaaatgggc tttaaagtag gtgtgctgga tcctgtgagc gattgtccag
      841 ccgcacaagt tgcagattgg cacattgaaa gtgtctatga tgacaccttt gcgctgaaag
      901 agcttgccag aagaacagat gtcattacct atgaattcga aaacgtcagt gtagaggcgt
      961 taaatgcgat tttacccatg tcatttattc ctcaagggac agacttgctt gcgattacgc
     1021 aagaccgcct gttagaaaaa tcttttttgg aaacgaataa catcgtgatt gctccttatg
     1081 caacgatcgt cagtccgacg gatatccaag atgcgattga cgggatcggc tatccttgtg
     1141 ttttgaaaac cactcgaggc ggctatgatg gcaaggggca gtatgttttg aaaagtcggg
     1201 cggatctagc tcctgccatg gaccttttaa gagaaggaac ctgtgtttta gaagcatgga
     1261 tcccgtttga aaaagagatt tcgatcatgg tggcaggaaa tggtcaagga gatttcacga
     1321 cttttccggt cgtggaaaac atccaccaca acaatattct tcatgaaacg attgctccag
     1381 ccgcgattga tcaggatgtg atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag
     1441 cagtgagcct ttcaggtgtt ttaggggtag aaatgttttt aacaaaaaca ggcgggctat
     1501 atgtgaatga attagctcct cgtcctcaca attctggaca ttattctatt gaagcttgtt
     1561 ctatgagtca gttcgatgcc catattcgag gtatttgtgg ttggccatta ggagaaaaag
     1621 tacgtttgat aagcaatgca gtaatggtca atgttttggg agaacaactg ctggaaagct
     1681 atcgcttgat tgagaataag ccagactggc aattccattt ctatggaaaa aaacaagcaa
     1741 aaaaaggtcg caagatggga catatcacta tcttgacaga agacatccag caaacgctgg
     1801 aagaaatcaa acaaacgaat atttgggatt agaggagaaa aaacatgtta gaacgttata
     1861 cacgccctga aatggggaat atctggacgg aagagaacaa ataccgtgcg tggctcgaag
     1921 tcgagatttt agcagatgaa gcttgggcag aacttggtga aatcccgcaa gaagatgtga
     1981 aaaaaattcg tgagcatgca tcatttgata tcgatcggat cttggaaatc gaaaaattaa
     2041 cacgccatga tgtcgttgcg tttacacgag cggtttctga aacattggga gaagaaagaa
     2101 aatgggtcca ttatggtcta acaagtacgg atgtcgtaga tactgcttat ggctatctca
     2161 tcaagcaagc aaatgatatt ttacgggaag acttgaaacg ttttacggaa atcattggcg
     2221 aaaaagcaaa agaacacaaa tatacagtga tgatgggtcg gacacacggt gtccatgctg
     2281 aaccaactac ttttggattg aaacttgcca catggtattc agaaatgaaa cggaacatcg
     2341 aacgatttga acatgcggca aaaggagtag aagcaggaaa aatctcagga gcagtgggaa
     2401 cgttcgcaaa tatcccgcca tttattgaag agtatgtatg taaacaattg gggatccgtc
     2461 cacaagaaat ctctactcaa gttctccctc gggatctaca tgctgaatat ttctcagcga
     2521 tggcgcttat tgctacaagt attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat
     2581 ccgaaacacg tgaagtagaa gaattttttg cgaaaggtca aaaaggttct tctgctatgc
     2641 cgcacaaaag gaatccgatc ggttcagaaa atatggctgg tctagccaga gtggtgcgcg
     2701 gacacatggt cactgcctat gaaaatgtca cattatggca tgaaagagat atttctcatt
     2761 cttcagcaga acgaatcatc ttgcctgata ccacgatttt attgaattac atgttaaatc
     2821 gttttggcaa tatcgttaaa aatctaacgg tctttcctga aaacatgaag cggaatatgt
     2881 ctgcaacatt tggtttgatt tatagccagc gcgttatgct gaaattgatc gataagggaa
     2941 tgacaagaga agaagcttat gatcttgttc agccaaaaac tgcgtacgct tgggacaacc
     3001 aagtgcagtt ccgcccgctg ttagaagaag atccaaccat cacatcggta ttatctaaag
     3061 aagaaataga cgatgccttt gattatcatc atcatttgaa aaacgttgac gtgatattca
     3121 aacgagtagg tttggaataa agaagagtga gttgcaaaat tcaattaaaa tagaatagca
     3181 aaaataacca aaaaagaagc agatccgtta gcggttctgc ttcttttagt ttattatatt
     3241 ttcttatcaa taataggaac ctgattttaa aagcggctac ctctttaccg tgcctcttta
     3301 catacaaaaa atagaggaat taagcgtaaa tggcttggct attataacta aaatgctggt
     3361 tatggagatg ttgcagcaag ttttcgagaa aagcgtattc catctcttgt ccattgaata
     3421 agatctggat acgataatga tttttgaaac gcttctgttc atgaatctga ctgattgaaa
     3481 gttcgccttt gtgttctgtc tgataaagaa aagtcctttt acggcattca gcacagcgct
     3541 tggttgctcg gcggattttt cggaatgtat aaaaactagt atctttattg cataaataaa
     3601 cgtctttgat cgacagcaaa tcattatata gacaggaacg ttcttcagct gttaattctg
     3661 ataaaaaaag ctgaccttgt tttattgcat cttccatcgg caagtgcctc ctcgaaccca
     3721 ttatccaaat ttttagttaa ttctaatata ccatcattaa gtataaatgt aaacgcttgt
     3781 tgatggtata agattttcgt ttctgataaa acacgaacat taaaagaatc taatgcctta
     3841 aatgtacata tttttgttga tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac
     3901 gaacaaaaaa cgatgtattt gcttttaatg taaataaaaa ggagagacgt acatggagaa
     3961 gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat cttacttctg atgagaactt
     4021 gatttggata gaatatttag atcaagcaac tgcgttgaat ggcgcgagga aagaccagat
     4081 aagtggaaaa ggagaattga ataatcaaat cacaagtgca attttccgtt atctgactca
     4141 aagaggaata aaaaatcatt ttattgaaca attgtctaaa accgaacaat tagcaaaaaa
     4201 agtacaaatt atccctctgg aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa
     4261 acgtctaggt gttcccgaag gtaaagaatt catcaaacca atcattgaat tctactataa
     4321 ggatgaccag ttggatgatc catttatcaa tgaagagcat atctatttct tacaacttgc
     4381 aaagccggaa gaaatcagtg agctgaaagc ccaagcgttg gctattaatg aaattttgac
     4441 cgaattattt gctaaggtcg gtttggtttt agtagatttc aagctagaat ttggccggtc
     4501 gaatggacaa attttgcttg cggatgaagt aacaccggat acttgccgat tatgggatga
     4561 acgtacgaat gaacatatgg acaaagacgt ttaccgcaga gatctaggtg atttgatccc
     4621 agtctatcaa gaagtatata accgtctatc ggaactaact gaagaaaagg agcgagtatg
     4681 atgtatttcg taaaagtata tgtaacctat aaagattcag tgttagatcc tcaaggggag
     4741 gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa tcgaagatat ccgaatagga
     4801 aaatactttg agatacaact tgctgatgca gaagaaccaa tcgatacagt gattgaaacg
     4861 atttgtgaca agctgcttgc aaatgtaaat atggaaacat accgatatga aatcaaaaaa
     4921 atggaggaag tataacatga aatttgcggt catcgttttt ccaggttcaa attgtgacgc
     4981 agatatgtta tgggcagttc gcgagatcat gggcgctgat gccgaatttg tacggcatga
     5041 tgagtcctct ttagaaggat ttgatggggt tttactgccg ggaggattct cctatggtga
     5101 ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc atggaagaag tgattcgttt
     5161 tgctgaagaa gggaaaccag tattcggtac atgcaatgga ttccagatat tgacagaagc
     5221 aggacttttg ccaggtgtac tgagacgaaa tgagtctttg cattttattt gcaaaactgt
     5281 accattgaaa gtcaacccca agacggcctt tacttcagag tatgaagccg acgaaataat
     5341 ctatcttcca gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga
     5401 actaaaaaaa aataatcaga ttgcctttac ttataatgac aacatcaacg gcagcgtgga
     5461 aaacattgca gggattacga acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga
     5521 acgtgcagta gaagacttgc taggatcaga agatgggaaa cggttctttg cttccatttt
     5581 aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg aaagaaccga cagctgaaga
     5641 gatcaagaaa aaaagaatct atgccgaatg gggattgact gatgaagaat atcgaatgat
     5701 cgaagaagat atcttaggaa gaatgcctaa ttatacggaa actggactgt tttctgttat
     5761 gtggagcgaa cattgttcct ataaaaattc taaaccggtt ctacgcaaat ttcctactag
     5821 tggacctcat gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg
     5881 actagcagtt gtcttcaaag cagaaagtca caatcatcct tcagccgtgg agccttatga
     5941 aggagctgcg acaggagttg gcggaatcat cagagatatt ttcagcatgg gggctcgtcc
     6001 aattgcgttg ctagattcat tgaggttcgg cgaattagat aatccaagaa cgaaatattt
     6061 actagaagaa gtagttgcag gtatcggcgg atacggcaat tgtatcggca taccgactgt
     6121 aggcggcgaa attgcatttg atccttgtta tgaaggaaac ccattagtca atgcaatgtg
     6181 cgtaggtttg attgaacaca aacatatcca gaaagggcaa gccgctggcg tcggtaattc
     6241 gatcatgtat gtaggtgcaa aaacaggtcg cgacggtatc cacggagcaa cttttgcctc
     6301 agaagaattt aatcaagaag aagaacagca acgttctgct gtacaagtag gcgatccttt
     6361 tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt cggattctct
     6421 aataggtata caggatatgg gagcagcagg acttgtttca tctagtgcgg aaatggcttc
     6481 aaaagctggc tcaggactta tattgacatt agatgaagtc ccacaaagag aaactgggat
     6541 gacaccttat gaaatgatgt tatcggaatc acaagaacgg atgttgattt gtgttaaatc
     6601 aggagaggaa gaaaaaatcc aagaactatt tcagaagtat gatctagatg cagtcactat
     6661 cggtaaagta acagacgacg ggcaatatcg gctatatcat caaggagaag aagtagcaaa
     6721 tctgccagtc gatgcattag ctgaagatgc accagtttat cataaagaaa tgaaagaacc
     6781 agctcgaatc gtagaatttc agcagttagc tccttatcag ccagttatcg aagaacctgg
     6841 acaagtattg cttgatctgc tgcagcaacc gacgattgct tcaaaacgtt caatctacga
     6901 aacttatgat tcacaagtac aaaccaatac agttgtccgt ccaggaagcg atgctgctgt
     6961 gatgagggtt agagggacga ataaagcgtt agctatgacc acagactgca acgcgcgcta
     7021 tctgtatttg gatccaaaaa tcggaggaca aatagcagtg gcagaagctg ctagaaatat
     7081 tattgctagc ggtggtaagc ccttggcgat caccgactgc ctgaactatg gttctcctga
     7141 taaaccagaa gttttttggg aattatcgac ttcagctgac ggaattgctg ctgcgtgtga
     7201 aacgttagga actcctgtta tttcaggaaa tgtctcttta tataatgaaa cggatggtca
     7261 agcaatctat ccaacaccaa tgattggtat ggtcggattg attgaggatc ataaacatat
     7321 cactactcag gaattcaaaa aatcagggga tctaatctat atattaggta agacgtttgc
     7381 agattttgat ggcagtgaat tacaaaagat gcagcttgga agaatcgaag gtgtgatccg
     7441 aaactttgat ctatccattg aaaagagaaa ccaagagtta gtattgacag ccatccaaaa
     7501 cggattgatt gaaagtgctc atgactgttc agaaggtggc ttggcaattg cactggcaga
     7561 atcagctttc aaacatcagc tagggatatc tgtacagttt gaattgtcct ctgctcaatt
     7621 atttgcagaa actcaatcaa gatttgtgtt gacagtcgca ccggaaaata agacacgatt
     7681 cgaagaaatg atgggagatg ctgctgtttt agcaggtaag gtgacggatg aagcgataat
     7741 cgaaatttca gcaactgacg gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg
     7801 ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg cgggatcttc
     7861 ggtgtttggg gtcatccaga cgccgctagt gtggcatact tcgggctcca tagtcttcag
     7921 catcgaggac aagaaggtgc aggtatcgtg tccaatgatc gaggaaagct taaggggcat
     7981 cgagatttgg ggctgctttc tgaggtattc aaagatcaaa gaaaattgca gcagttaaca
     8041 ggagaagctg caatcggaca tgtacgttat gcaacagcag gcaatggaag tgtggacaat
     8101 attcagcctt ttctattcaa atttcatgat cagtcgatcg gcttagcgca taatggtaac
     8161 ttgatcaatg ctcgttccct tagaaaagag ttagaggaaa atggggcgat cttccattct
     8221 aactccgata cggagatatt gatgcatttg atccgacgaa gtgaagaaac agatttttta
     8281 aataaattaa aaagtgcttt gaatgaagtc aaaggtggct ttgcctattt attaatgaca
     8341 gaaacggcaa tgattgccgc tttggaccca aatggttttc gtccattagc aatcggacaa
     8401 atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg cattagaagt tattggggca
     8461 cgttttataa gagatgtcca gcctggtgag atagtgatca tcaatgacga aggaatccag
     8521 attgattgct ttacgaagga cacgcagctt tctatttgtt cgatggaata tatctatttt
     8581 gccagaccag attcaaacat cgcagggatc aatgtgcata ctgcacggaa aaatatggga
     8641 cggaatttag cgaaagaatc gccagtaaaa gcagatatgg tcgttggcgt acctaattct
     8701 tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga aatcggatta
     8761 gtaaaaaatc aatacattgc ccgaacattt atccagccaa cgcctgagct aagagagcaa
     8821 ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg
     8881 gtcgatgatt caattgttcg gggtacgaca agcagacgga tcgtccatct tttgaaagaa
     8941 gcaggggcaa aagaagtcca tgtgaggatt gcttctcctc ctttgaaata tccatgtttt
     9001 tacgggattg atattcaaac gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa
     9061 atcaaagaat gtatcggtgc agattcttta ggttttttaa gtgaagaggg tctgatcgaa
     9121 tcgatcggtc tggaaaaaga tgctccgtat tcaggattgt gtatggctta tttcaacgga
     9181 gattatccga cgccgttata tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa
     9241 gtatcgttct tttaaaagca gaccaagaag ggagtatatg aattcaatgg caaatgcata
     9301 cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta gtagaacgga tcaagaagca
     9361 tgtcaaaaaa acggaacgtt taggtatgat gggcgctata ggaggattcg gcggttgttt
     9421 tgatttatcg gtctttcaat taaaagagcc agtccttgtc tcggggaccg atggcgtggg
     9481 gacgaaatta gcagtggcaa tcgaagcaga caaacacgat acaatcggga tcgactgcgt
     9541 agcgatgtgt gtcaatgata tcttagcaca aggcgcagag ccgttatatt tcttagacta
     9601 cttagcaacg gggaaaaatg atccgttacg tttggaaaat gtagtaaaag gtgtagcaga
     9661 tggatgtgtt caaggaaata tggcgctgat tggcggagaa acagctgaaa tgcctggtat
     9721 gtactcaggt aatgattatg atctagccgg atttgctgta gggatagctg aaaaatctca
     9781 attattgaca ggagaaacga tcaacccagg agatgtgttg cttggaatag catctagcgg
     9841 gattcattca aatgggtact ctcttgttcg caaaattttc tttgagcagc agcagttttc
     9901 tttatcatca aaactgccag aattgaaaaa aacacttggc gaagaattgc ttgagccaac
     9961 gaaaatctat gtttcttcct tgcttccgct gatcaaagaa aagaaaataa aaggagctgc
    10021 acatatcact ggtggagggt ttgtagaaaa tatcccgcga atgttaccag aatctatggc
    10081 tgcacaaatc aaacttggct catggccgat tcttccaata ttcggctgtt tacaaaaata
    10141 tggtgaaatt cctgagatgg agatgtatga aatcttcaat atgggcatcg gtatggtcct
    10201 ggcagtcgaa gaatcgaaag taaaagaaat agtacacgaa attgagcaag ctggcgaaaa
    10261 agtttatgaa atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga
    10321 agaacgatga gaatcgctgt ttttgcttct ggaaacggaa gcaactttca agcgctagcc
    10381 gattatctat cgaaaaaagg attggaatct tcgattgact ggttgttttg tgatcagcct
    10441 gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg tttttcacct
    10501 aaagaatttg attcaaaaaa agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa
    10561 aagatcgatt tgatcgtatt agctggatat atgcgaatca ttggaccggt attgctgaaa
    10621 aattatgata aaagaatcat caatatccat ccgtctcttt tacctgcctt ccctggtttg
    10681 catggcatac gtgatgcctt tgaagctgga gtaaaagaaa cgggtgtcac aattcattat
    10741 atcgaccaag gtgtagatac tggaccaatc atccggcagg aaaaagtgag aattgaacag
    10801 gaggacacgt tcgattcttt agaagaaaaa atccatagag tagagcatcg aatctatcca
    10861 gaagtgatta gtgaaattat tgaaaatgga ggctatctga aatgacaaga gcattgatca
    10921 gtgtatcaga taaaactggg attgtagcat tcgcccaagg tttgattgaa caaggagtag
    10981 aaatcatttc aacaggagga acaaagcatg cgttggataa agctgggatc aaaacgcttt
    11041 cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc
    11101 ctaaaatcca tggtggtctt ttaggaagac gcgatctccc tagccatatg gaggcaatgg
    11161 aaacggaaaa tatccaacca atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa
    11221 cgattatgaa accagatgtc gaaatagcag aagctattga aaacattgat atcggtggtc
    11281 ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt gttgatccat
    11341 ctgactatga agaagtactt tcagaattac gtcagtatgg gcatacttca ctttctttca
    11401 gacaaaaact agcggcaaaa gtctttcgtc atactgcggc atatgatgca ctgatcgctc
    11461 aatacttaac ggattgggtg gaagaaaaag aaccggaaaa attgacactt acgtatgaac
    11521 gaaaacaaac gcttcgttac ggtgaaaata gccatcaagc cgctgccttt tatcagtccg
    11581 ctcttctgga aaaatattcg atcgcagcag caaaacaact ccatgggaaa gaactttctt
    11641 ataataatat ccgagatgca gatgctgcgc tacggatcat gaaggaatat gacgagccaa
    11701 cggtagtcgc cttgaaacac atgaatcctt gtggtattgg gacagcatct acgattttag
    11761 aagcgtggga agcagcgtat gaagcagatc ctgtttcaat ctttggtggg atcatcgttt
    11821 tgaatcgaga agtagatctg ccaacagcag aacagatgca taagctgttt ctagaaatca
    11881 tcattgcccc ttcttataca caagaagctt tggaattact gcaaaaaaag aaaaacatcc
    11941 gattactgac agtagacttt gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag
    12001 tcttaggtgg acttcttgta caagatcaag atctggcaat agaaaatgaa gaagagtgga
    12061 aagtcgtgac aaaacgtaag ccgactccag cagaagcaaa agcattagcg tttgcttgga
    12121 gagctgtcaa acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt cagacagtag
    12181 gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc
    12241 aagagaaaat ggaaggtgca gttttagcta gcgatgctta ttttccaatg gatgattcgg
    12301 ttgaatatgc agccaaacat ggtataaaag caatcattca gccaggagga agcatcaaag
    12361 accaagattc tattgatatg gcgaataaat acgatctagc aatggtcttt actgatgttc
    12421 ggcattttag acattagaga ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg
    12481 gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc gaaggtggat acggtgtatt
    12541 gtgcgccagg aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc
    12601 gagatcattc agcgttaatc cattttgtta agaaagaggc tattgattgg agtatcgttg
    12661 gtcctgaagt tcctttgtta aatgggatcg tcgatgattt tatggcggaa ggattgaaaa
    12721 tcttcggtcc gacaaaagca gcagctctga tcgaaggttc gaaaagtttt gctaaacaat
    12781 taatggatac ttatcaaatc ccgacagcta aatccagaag ttttgaatgt tacgaaaaag
    12841 cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt gaaagcggac ggcttagcag
    12901 ctgggaaagg tgtagtcgtg gctaccacag tgaaggaagc gctggatgcg gcagaacaaa
    12961 tgttgaacca gcatcgattt ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg
    13021 caggagaaga attctccctt ttggcgtttg tccgtaatac caaggtgtat ccgatggtca
    13081 tttcgcaaga tcataagcgg gcatttgata acgataaagg gccaaataca ggcggaatgg
    13141 gtgcttattc accagtaccg caaatttcaa aaaagatgat tgaaaaagct gtggaagaag
    13201 ttttaaaacc agcggctttg ggaatgaaag aactaggacg ctctttcacg ggtgttttat
    13261 atgcgggttt gattgctacg aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg
    13321 gtgatcctga aacgcaagtc gttctttctc ggttgaagag tgactttgcg caagtaatcg
    13381 atgatttatt agagaatcgt acagtagagt taaagtggca gcaagaagga tacaatgtag
    13441 gcgttgtggt tgcagctgcc ggttaccccg aagaatatga gacaggaatg gttctaccag
    13501 attttctgga agaagagctt tctgtttact atgctggagt ttcctcaaat aacggcaaac
    13561 tagttggagc aggaggacgt ctttatttag tagaagcata cggtgagacg atcaaagaag
    13621 cacagaaaaa aatttacacc gcgttaatgg ataaaacgac agatggaaca ttttatcgta
    13681 cagatattgg agaaaaagct gccaatagcg aactgatcgt gaagtaaatc agctgcttcc
    13741 atttataaaa aatacaacaa acataatcgg aagcgccgat tttagtgaag ccttttcata
    13801 aagagtgtgg tacacttttg ttgtgtatat aaataaggag gagttcagat gaaaaaaatt
    13861 caattaggta cttctgatca gaaagcagct gcagtgatct taggctgtat gcgtatcaac
    13921 aatgcggaag atccagtaaa agtgattgag acagcttatg aaaacggcat tgattttttt
    13981 gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca
    14041 aaaacatcaa tcaaacgaga agatcttttt attcaaacaa aatgtggaat cgttccaggt
    14101 gtgatgtttg atttttctaa aaaacatatc attgaatctg tagaaaaaag tttgacccgt
    14161 ctgaaaatgg attatgtgga tgctttactt cttcatcgtc cagatacatt ggtagaacca
    14221 gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg ttattttggc
    14281 gtcagcaatc aaaaaccaat gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta
    14341 gtagctaatc agctgcaatt tggcttgaaa catactggaa tgatcgatca agggattcat
    14401 gtcaatatga cagatgaagc aagtatcgat catgacggta gtgtacttga ttattcccga
    14461 ttgcatgata tgacgatcca agcatggtca ccataccagt atggattttt tgaaggtgta
    14521 tttatcggga acgaaaaatt ccctgaatta aaccaaacat tagaagaact agcagctaaa
    14581 tatcaaacga caccaactgg cttggcttct gcttggattt tacgccatcc agccaatatg
    14641 caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa
    14701 attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct
    14761 taaagattgt cataagcagg tcaaaagggc tataaattaa aaagacagaa taacttgaaa
    14821 gctgttttca gcttcgagat attctgtctt tttttattgc aaatggaaag agtatcattg
    14881 tacggatttc gtttattagt tatttttgtt tttaaggaaa agctgtgtcc gatattcttt
    14941 tggagaaatc ccttgttttt tcttaaataa ataactgaaa tagttagggt cattataacc
    15001 aatttcaaaa gcaatatctg aaatacgata gtctgattgc tgcaataatt tttttgctaa
    15061 ttcgattctt tgttctgtca gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat
    15121 cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg acccaagtta aggacatatc
    15181 tggatcggta aaattttctt tgataaaatc caatgcatgg ttcaaaattg gctgatattt
    15241 gttccttgaa ggctgtgagg gcagagcagt taagaaagaa atgaacatat aaactgtcga
    15301 acggtaaagc gagtcgtcgg cgctgattgc taagcgttga ctggtggatg tgcaatgaac
    15361 catttcttgc gggattttct tttctttcag gcagacatgc tggagtagct tagtcaagac
    15421 aaataaacga tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat
    15481 gtacttagat acttcgtttt tcgataatga agacaaatca agtgagaggt caaagtaaaa
    15541 gacagaatca actggaagtt tcgcttcttg aatcaaatcc tcatagctga taatttgttc
    15601 tttaagggaa ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg caggtaattc
    15661 gctgattctt tctgcgacag gaccaatggc gacagaaaaa ttattttttt gtcccttttc
    15721 taattcatgg accaacgttt gggctacctg ataactggtc tcgatcacat tttcttcaag
    15781 agaatcaact attaaaaatt tgatgaatcg agaggaaaca gcagagaaga tcacttgttg
    15841 attctcccta aataaaaact tcaaataatc actgagatga gcgtaatctt caaagtctgt
    15901 actgacttgg tttgctgcta ataatacggt catttttttc cctgctaaag agcgattgaa
    15961 agattcagca agtttcaatg tttcactgat cgtaagtgag cctttgaaca aaccgttcaa
    16021 gaaatgattt tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg
    16081 caaatctaaa gcagttactg ctttttctat tgcttgtttc aattcgctac ttttaatcgg
    16141 tttaagcaga taatcatctg cctgtacctg gattgctcct ttcatataat cgaaatcgtc
    16201 gaatccacta ataaatatga gacgagtcca aggaagtagt ttccttgctt cttttgaaaa
    16261 agtcaacccg tccatgaaag gcatttggat atcagtcaga atgatgtcgg gctgtaagtc
    16321 gataattgaa gacagtgcca tctcaccatc tccagcttcg ccgacataag tgattggata
    16381 ataagaacca aggacagtta tttgttcccg caagttattg cgaatgaggt attcgtcttc
    16441 gatgataaaa actttataca tgtttgtttc ctccaattgt tttgggagat agagggatca
    16501 tcagtgtgac agttgttcct tctcggtatt tgctgtcaat cgtgataggt ttttgatcat
    16561 ttccgtaata aagaaggaga cgcttgttta cgttgtaaag tccgtagccg atactgaaat
    16621 ctgaatcgat ccctttggct aactctttac gtatttctgc cagtttttct gcagggatgc
    16681 caatcccgtt gtctataact ttgaattgga tcatctcatc aattttgcat gtggtaatac
    16741 gaataaatcc tttgtgacgg acgtacttcg ttccatggta tacagcattt tcaatcaatg
    16801 gctgcaagat catttttaaa atagaaacag gaaaaagtgt ttctggtata tcgatcttat
    16861 agtcaagcat atggccgtat cttattttta agatgactag ataatcattg atatggctaa
    16921 tttctttttc aagaggaatc cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg
    16981 aaagtgcatg ggtcatagtc agagcttctt caaagcagtc ttgttcgatc aaagaaataa
    17041 tggcatccaa actgttataa ataaaatggg gagtaatctg agcttgcaat acacgaactt
    17101 cactttgtgc taaatggtat tgtttcaaag cgttctcccg taaagactgg tttagatctt
    17161 ccgccatttt attcatgcta gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta
    17221 aacgaaagcc aaggttccct gaagaaagtt gttctgacat ctcgatcagc tgattgatcg
    17281 gttcttggac ctttctctct aaaaagcgac gattcttttt cgcatagaaa aaaatggtac
    17341 taagaactcc aagttccact attgttaata gaatgagcga gcggacaagt gtcgtatttt
    17401 ttttgcttgc taagtcgatc tccacacgaa caaaatcttg taagatatct gacagcagtt
    17461 gagtgacaga atctacctga ttgatgatct cttcattggt atccataaaa tcttttgcca
    17521 agatattatt gatcagctcg ttttgataat cttccagtgt atcgatcgta cgtagcgata
    17581 catctaacgt actaatttct ttaagtgtat ttgtattatt tttgatctct cgaatatcat
    17641 tcctaagttc ttctaggata ttttcctcat tgaactcatc tacggttact tggccagaaa
    17701 tcaagtccca catactttcc aaaacttttt gatcaacctt caaagcaatt gcatctgctt
    17761 tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc
    17821 tcagtagcaa agggataaca gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct
    17881 gttttatgct taaatttttt gtcatgatac ttctccagtc aataatttct ggttgggatg
    17941 ttagctatat tctgctggga aaagacagtc tcggaaataa aaatatcttt aggtaatgtt
    18001 tttcctgttt gaatatttcc aaaataacga gtaacggtat tggctacata ccagccgaca
    18061 tcaggtttac actcgactac acaattgacg attcctgagc gtagcttctc gatcatatct
    18121 tcttgtccat caatagttac aatgatcaaa tcttctccag gtttgattga tgtttctttt
    18181 atcgcagcta aagcaccgtg agtcatttca tcactatggc tgtataatac atcgatttcg
    18241 gataaatcat ttgtttctaa atatcgtttc atctggtctt ttcctttcat ccgaataaaa
    18301 tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt tccatctttt
    18361 tttagggttg gtttacgatt gattgattcc aaaaatcctt cggagcgtag cttggtcggt
    18421 gtggagttct ctgaaccagc aagttctagg attctgattt cctgtttctt tgaatttgtg
    18481 aagtgattgc tgacaaataa acccgcccga tttccttctg ctttgaaact ggggccaatg
    18541 tgggtgacga aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg
    18601 atccctgctc gctttgcttc ttttaataca gagtcccacc cggattcttg caaaggagta
    18661 aaaatgatca tatccacttt ataggcaatg aacgtccgta tatcttggat ttgccgttcc
    18721 tgattcatgt agccatttcg gtagagaact tggtaatctt ctttttcaag cgcagtgata
    18781 attgattcag tatgcttttt ccgccagcta ctctccgttc ctgattgcga aaaaccaacg
    18841 actagtgggt caggtgcttc ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt
    18901 acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa atcactccat ctggatttgt
    18961 tagtactatc ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa
    19021 tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat
    19081 cccataaaaa aatcaatgtt aacgctttct ttattagcta tactcggtgt atcaattgaa
    19141 atgggggata aaaaatgaaa aacaaattta gaaagattgt tgggactatc gctattttga
    19201 ccgcaagcat tctattaagt gcgtgtggca attcaggatc tgcagatgat agtacggggt
    19261 atgtaggaat cgctatgccg accaaatcag cagaaagatg gatcgctgat gggaacaaca
    19321 tggtctcgga attagaaaaa ctgggttata aaacagatct tcaatatgga gaagataaag
    19381 tagaaaatca agtagcacaa atagaaaata tgattacaaa aggggtagat acacttgtga
    19441 ttgcttctat cgatggttcg gctttaactg atgtgttagc taaagcaaaa gaagcagata
    19501 tcaaggtgat cgcctatgat cgtttactga tgaattcaga aaatgtcgat tattacgcga
    19561 cttttgataa ttttggagta ggtgtatcgc aagcagctta tattgaagag cacttaggat
    19621 tgaaagaagg aaaaggacca ttcacgattg aattatttgg tggctcgcct gacgacaaca
    19681 acgcactaat caattacaac ggagtgatgt ctgttttaca accatatatg gacaacggtc
    19741 agttggtcgt gccatctgga cagacgagtt tcagtcaaat cgctacgctt cgctgggatg
    19801 gttctacggc gcaagcaagg atggataatc tgttaagcgc aaattatact gaccaaacat
    19861 tggatgctgt tttgtcgcca tatgatccaa taagtttagg aatcatttcg tcattaaaag
    19921 gcgtggggta tgggagtgaa agtaaacctt tacctgtgat tactggacaa gatgcgacag
    19981 ttgctggcgt taaatcgatc attgccgggg aacaaacaca aacgatcttt aaagacacac
    20041 gtatcttagc aaaaaatacg attgaaatga tcaaagcaat cagtgatgga gaagaggttc
    20101 cggtcaatga tactgaaact tatgataatg gggtgaaaac tgtccctacg tatttagcaa
    20161 atacagtatc tgtcgataaa gataattatc aagcagagct gatcgacacc gattattata
    20221 aagaatccga tctgaaaaat taagaggaac caaacaagga actaaagaga tctaagtcag
    20281 agagaggata agaattatgg cggattgtat tttaaaaatg gcagatatca ccaaaaaatt
    20341 ttctggaatc cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc
    20401 attgtgcgga gaaaacggag cgggaaaatc aacattgatg aatgtcttga gcggcttgta
    20461 tccatatggc tcctatgacg gggaaatctt ttatgatgga aagctgtgtc aatttaaaga
    20521 tttgaaagat agtgaagaaa aaggcatcgt catcattcat caagaattag cactaagtcc
    20581 ttatctttca ataaaagaga atatttttct cggaaatgaa caagcagaaa gaggcgtgat
    20641 tgattgggaa ctgacagaaa agaaaacata tgaactgctg aataaagtcg gtttgtctat
    20701 cgacccgaat cttctagtat cccagattgg cgtagggcaa cagcaactag ttgaaatagc
    20761 taaagcatta tcaaaatccg tccgtctgtt gattttggat gagcctaccg ctgcattgaa
    20821 tgaagaagaa agcgcgaatt tgctgagatt gatccgagag ttgaaagagc aaggtgtaac
    20881 ctccatcatt atttcacata agctgaatga aatcgtggat atcgcagatc gaatcaccat
    20941 tttacgggat ggacagacga ttgagacact agaaaatgaa atgatcagcg aagagcatat
    21001 tattcgcgga atggtgggca gagatttagt tgatcgttat cctgaaagaa aaccggatat
    21061 tggagaagtt tattttaaag tagaagactg gacggtctat caccctcttg atcacgagcg
    21121 aatcataaac gaccacatca acttgcattt gaaaaaagga gagattgtag gaatagctgg
    21181 actgatggga gcgggcagaa ctgaattcgc tatgagtcta tttggacatt cttatggaag
    21241 caagatatcc ggacgtatat taaaggaagg ggcagagata tcagttaaga ccgttccttc
    21301 agccattgca aacggactcg cttatgtctc ggaggaccga aaatctttag gactgaatct
    21361 gctgatggat attcgggaaa acgtgacgct gagcagtttg gataaaataa gtcaaaaagg
    21421 agtattagat aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa agatgcgtat
    21481 aaaaacaaac tccatccatc aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt
    21541 cgttttatcc aagtggctga tgacacaacc cgatattctt tttttggatg agccaacccg
    21601 tgggatagat gttggggcaa aatatgagat ctacacgatt atcgaagaaa tggcagcaaa
    21661 tggcaaagct gtctgtctta tctcttctga acttcctgaa atattaggga tgtgtgatcg
    21721 aatctatacg atgaacgaag gacgtattac aggagaagtc tcgcgagaag atgcagatca
    21781 ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt taagttggaa caggcaaaaa
    21841 atcttacgga taaaaaagag aaaaaactaa atttgaaaag tagagcagct gatctttttg
    21901 gtcgctacag catggtgatc attttagcgg gactacttat tgcgtttcaa ttgatgacgg
    21961 atggtatttt ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat agccatatat
    22021 tagtactagc tgcagggatg ttattagttg tgctcttagg tcatgtggat ttgtcggtag
    22081 ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat cgccaaatga
    22141 acccgtggat gacagttatc gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag
    22201 gtttttgggt agcttatgca gggataccgg cgtttatcgt gactttagca ggattgctga
    22261 tgtttcgtgg actgacacaa gttgttctag gaggccaatc tctagcacct tttccagctg
    22321 ttttccaaaa aatgtctacg ggttatttac cagacttaag cgaaggaagc agccttcact
    22381 tcttgacatt gattttagga atgatcatca cgatcgtgtt agttgttggt caatggcgaa
    22441 caagaaatcg tcggaaaaat catttatttg aagtggaatc tccaacagct ttcttggtaa
    22501 aggcggtatt tacagcagtc gttgtactag gaataactta tatctttgcc tcttatcaag
    22561 ggtatccgat tattttgatc attcttggtt tgatcgttgc agcatatggg tttttgacaa
    22621 ataaaacagt agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac
    22681 tatcagggat caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg
    22741 cagctttagc tggattagtt ttagctgctc gattgaatgc ggcaacacct caagcaggta
    22801 catcgatgga actggatgca atggcggcag tatattttgg tggtgcttcg acttccggcg
    22861 gcattggaac aatcatgggt gcgattgtcg gtggcttggt catgggtgta ttgaacaatg
    22921 gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc aatcaaagga ttgatcttgc
    22981 ttttagccgt tgtgctagac ttgtataata agaaaaaacg aacaacgtag gagaacgccg
    23041 gttattcaga aaaaagactg tgacaagaca tttgtcacag tctttttttc gctaaaagcg
    23101 aacattcatt gtttcgtttg tatagactaa aaataatata tgttttttta ccattcgtat
    23161 aaattctctt tttgctcttg atttttttgc ttaattgagt taaattacat agggcgctaa
    23221 aaaaagcctt gattaaatta ataatgttcg tgttttgctg gatgtgaaca tcaggaggat
    23281 aatgtaatga aagtatttga ttacgaagat attcaattaa ttcctaataa atgtatcgta
    23341 aatagccgtt ctgaatgtga tacaacggtc acattaggta aacatacatt caaaatgcca
    23401 gtagtgcctg ccaacatgca gacgattatt gatgaaacaa tcgcagaatt cttagcggaa
    23461 aatggttatt tctatatcat gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa
    23521 aaaatgaaaa agcgtggatt gattacctcg atcagtgttg gcgtcaagaa agaggaatat
    23581 tcctttatag agaagttggc agaagaatcg ctaaatcctg attatatcac tatcgacatc
    23641 gcgcatggac atgctaactc agttattgat atgattcagc atattaaaaa atatttgcct
    23701 gaaacttttg tgattgccgg taatgttggt acaccggaag ccgtccgtga attggaaaat
    23761 gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc
    23821 aagacaggct ttgggactgg aggctggcaa ctggcagctc ttagatggtg tgcgaaagca
    23881 gctcgcaagc cgatcatcgc tgacggtggg atcagaacac atggcgatat tgcaaaatct
    23941 gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca cgaggaatct
    24001 cctggcgaaa caaaagtcga aaatggaatc gtgtataaag aatactttgg ttctgcttca
    24061 gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa
    24121 ggatctttga aagatacact agtggaaatg caacaagacc ttcaatcatc tatttcttat
    24181 gctggaggaa gagatctgga agccattcga aaagttgact atgtcatcgt gaaaaattcg
    24241 atttttaatg gagacacaat ctaaaaaata aataaaatat taggatgttg tgaaaaagtt
    24301 gtcctgcatt aagtaataag aacagccaaa caaaaatagt tctttatatg ttttgtttgg
    24361 ctggaattat tatcgcagat acaagctttt gaacatcatt attcagaata agggactgtg
    24421 ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa
    24481 attcggtaaa atcaaaagaa aaaatagaca atagaaaaga gaagcttatg tgggaaaaag
    24541 tagagaaaaa acaaggaccg atttatcgtc aaattatgga acagattatg agagaaatcg
    24601 aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt
    24661 ttggtgtgaa tcgttccacc gttgtccatg cattggaaga gcttagggag cttggggtac
    24721 taacgagcaa acgtggcagc ggatggtttg ttaatcagac tgaatggggg aaattcgctg
    24781 ttccaagaat cgattggcgg caaatgatct ctcctcgtta tgagcagacg gactggtatg
    24841 agcaaaaaat aagagaagca aagcagatcc aaaaaacatc gtttcttgat ttatacgcta
    24901 gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa
    24961 tcttagcgga agaaaaagaa atggatcttt taggctatac tccgttacgt aacaaaatca
    25021 aacggtattt ggaaagtaaa tttgaattgg cattttcccc caaccagctt cttgtgactg
    25081 gtggcggtca gcaagcaatc tttttgattt tacaaacgat tctttcagct ggagagggga
    25141 tagctgtaga gtcaccttct tttttttacc gattacctct tttcaaagct tcaggaacgc
    25201 gtttatttgg gataccgatg gatgaagaag ggatcgactt agaaaaactg aaagaatcga
    25261 tccataaaaa taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa
    25321 aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc
    25381 caattattga agacgatgta tttacggatc tttcatttct aaaccaagaa gaagtccttc
    25441 ctatacgaac gattgatccg gataatgtgt tatacgtcgg ttcgttatct agggttttag
    25501 gaaaaacaac gaaaatcggt tgggtgatag gacctgagac ttttatctcg caactagctc
    25561 aagcgcaaga aatgatggag ttttcgatga atatccttac acaaatcaca gtggctaatg
    25621 tatttaatga agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa
    25681 gccgactgat aagcgcttgg ggagattcac aatcgttttt caagcttcat tcaattttag
    25741 gtggttacta tgcctggatt acttgggatg ggaaaaaact agaccccgaa ctagcagaaa
    25801 aagtagtaga tatgggtctt ggaattgcac caagtctact ttttgggcaa gatataaatg
    25861 gaatcagaat caattacagt aggctggatg aaaaccaaat accgttgttt tattcaaaga
    25921 tgaaaattct agaaaagtgg ttaacagaat aatttcacaa agtaaataga ttaaagaata
    25981 taacagcttg gatttaaatt tcaagctgtt tctttttttt cggaaaaaga atacgatata
    26041 aagaaactaa gaaaaaacca ttatttgtac gtccgttcac gatgtgtaaa taaaataaat
    26101 gtttgcaaca aaactgtata acaccaacgt ttttaaatag aaacagatgg ttgtaagttt
    26161 gattagaaaa tagaaaaaat gcagatgaaa tataaaacgc tttccttatt gtgattttat
    26221 tcactaatgc tgttaatatg cggattggca taatttcctt tcatcgtgaa aatttgtgct
    26281 tttagcttgt atttctcata aatccatgat aatttattaa tgaagacggt taaatagtga
    26341 tacagtttta ttcactgttt caatcgcaga atgaagagat tttcttatag atgagagagg
    26401 aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact tccaagcact gctagatgaa
    26461 gtcaatgcag atttcccagt atatcaagca cttgatcaag atggaaacgt agttaacaaa
    26521 gatttagtac ctgatttatc cgatgatgaa ctcgttgaat taatgactca tatggtatgg
    26581 tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt
    26641 gcaccaactg ctggacaaga ggcaagtcag ctggctagtg cttacgcatt tgataaagaa
    26701 gatgttcttc ttccaggtta tcgggatgta cctcaactag taaaacatgg tttaccttta
    26761 tctcaagctt ttttatggtc tcgaggacat gctgcaggta attcttatcc ggaagaattg
    26821 aaagctctac caccacaaat tatcatcggt gcacaatatg tacaagcagc cggtgttgca
    26881 ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca cttatactgg tgatggcggt
    26941 tcatcacaag gtgattttta cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc
    27001 gtattttata tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa acaaactgca
    27061 gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc ctggtattca agtcgatgga
    27121 atggatccgc ttgcagtata tactgtatct aaaatggcac gggaatgggc gatcagtgga
    27181 aatggaccag tcctaatcga aacactgact tatcgttatg gaccacatac actatctggt
    27241 gacgatccaa cccgctaccg tacaaaagac acagatgacg attggcagaa aaaagatcca
    27301 ttgatccgct tccgtaaata tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa
    27361 caagtcatcg aagcaactaa agaagaaatc aaagcagcta ttgctgaagc cgacaaagta
    27421 ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg aagtatctcc acaatcaatc
    27481 aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa
    27541 tgatccaagc aatcacagat gccttagcac ttgaactaga aaatgacgag aatgtcgttg
    27601 tttttggtga agatgttggg aaaaacggag gagttttccg ggcaactgaa ggattgcagg
    27661 aaaagttcgg agaagaccga gtattcgata ctcctttagc tgaatctgga atcgcaggat
    27721 tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa ttctttggtt
    27781 ttattttcga agcaatggac gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga
    27841 gcggcactag aaatctaccc atcacaattc gttcgccatt tggtggcggt gttcatacac
    27901 cagaattgca ttcggataat ttagaaggtt tgatcgctca gtctcctggt attcgagtag
    27961 tgattccttc caacccttat gatgcaaaag gactattgat ttcttctatt agaagtaacg
    28021 atccggttgt tttcctagaa cacatgaaac tatatcgctc attccgagaa gaagtgccgg
    28081 atgaagctta cgaagttcct ttggataaag cagcagttac tcgtgaggga acagatgtat
    28141 ctattatcac atacggagca atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa
    28201 aagaaaatat atctgttgaa atcattgatc ttcgtacagt tgcgccatta gatgtagaaa
    28261 cgatcattca atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac
    28321 aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga acgcgctatc ctttcattag
    28381 aagccccaat cggacgagtt tctgcacctg atacagtctt cccatttgga caagcagaaa
    28441 atgtctggtt gccaaatgca aaagatatcg aagacaaagt aaaagaaatc gcggaatttt
    28501 aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt taaatt
//
LOCUS       DACEOM010000039        28509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEOM010000039 DACEOM010000000
VERSION     DACEOM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.040"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(218..1015)
                     /locus_tag="IW781_001587"
     CDS             complement(218..1015)
                     /locus_tag="IW781_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1182588.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IW781_001588"
     CDS             complement(1012..2196)
                     /locus_tag="IW781_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1182589.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IW781_001589"
     CDS             complement(2221..3468)
                     /locus_tag="IW781_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1182590.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IW781_001590"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IW781_001590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1182591.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IW781_001591"
     CDS             5448..5762
                     /locus_tag="IW781_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1182592.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IW781_001592"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IW781_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1182593.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IW781_001593"
     CDS             6447..7091
                     /locus_tag="IW781_001593"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1182594.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IW781_001594"
     CDS             7106..7435
                     /locus_tag="IW781_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182595.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IW781_001595"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IW781_001595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1182596.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IW781_001596"
     CDS             8423..9247
                     /locus_tag="IW781_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1182597.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IW781_001597"
     CDS             9240..9587
                     /locus_tag="IW781_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1182598.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IW781_001598"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IW781_001598"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1182599.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IW781_001599"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IW781_001599"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1182600.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IW781_001600"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IW781_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1182601.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IW781_001601"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IW781_001601"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1182602.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IW781_001602"
     CDS             15244..16011
                     /locus_tag="IW781_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182603.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IW781_001603"
     CDS             16001..18043
                     /locus_tag="IW781_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182604.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IW781_001604"
     CDS             18324..18473
                     /locus_tag="IW781_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1182605.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IW781_001605"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IW781_001605"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1182606.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IW781_001606"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IW781_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1182607.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IW781_001607"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IW781_001607"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1182608.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IW781_001608"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IW781_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1182609.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IW781_001609"
     CDS             22890..23972
                     /locus_tag="IW781_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1182610.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IW781_001610"
     CDS             23972..24775
                     /locus_tag="IW781_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1182611.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IW781_001611"
     CDS             24845..25585
                     /locus_tag="IW781_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1182612.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IW781_001612"
     CDS             25596..26600
                     /locus_tag="IW781_001612"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1182613.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="IW781_001613"
     CDS             complement(26645..28489)
                     /locus_tag="IW781_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182614.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         8992 a         5040 c         5863 g         8614 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaa
//
LOCUS       DACEOM010000040        26427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEOM010000040 DACEOM010000000
VERSION     DACEOM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.041"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(130..825)
                     /locus_tag="IW781_001614"
     CDS             complement(130..825)
                     /locus_tag="IW781_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1182615.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IW781_001615"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IW781_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1182616.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IW781_001616"
     CDS             complement(2024..2215)
                     /locus_tag="IW781_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1182617.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IW781_001617"
     CDS             complement(2236..3126)
                     /locus_tag="IW781_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1182618.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IW781_001618"
     CDS             complement(3113..3880)
                     /locus_tag="IW781_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1182619.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IW781_001619"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IW781_001619"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1182620.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IW781_001620"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IW781_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1182621.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IW781_001621"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IW781_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1182622.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IW781_001622"
     CDS             8496..8834
                     /locus_tag="IW781_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182623.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IW781_001623"
     CDS             complement(9002..9760)
                     /locus_tag="IW781_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1182624.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IW781_001624"
     CDS             complement(9778..10596)
                     /locus_tag="IW781_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1182625.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IW781_001625"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IW781_001625"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1182626.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IW781_001626"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IW781_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1182627.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="IW781_001627"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="IW781_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1182628.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="IW781_001628"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="IW781_001628"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1182629.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14615..14857
                     /gene="yaaA"
                     /locus_tag="IW781_001629"
     CDS             14615..14857
                     /gene="yaaA"
                     /locus_tag="IW781_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1182630.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14844..15968
                     /gene="recF"
                     /locus_tag="IW781_001630"
     CDS             14844..15968
                     /gene="recF"
                     /locus_tag="IW781_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1182631.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15965..17911
                     /gene="gyrB"
                     /locus_tag="IW781_001631"
     CDS             15965..17911
                     /gene="gyrB"
                     /locus_tag="IW781_001631"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1182632.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17931..20402
                     /gene="gyrA"
                     /locus_tag="IW781_001632"
     CDS             17931..20402
                     /gene="gyrA"
                     /locus_tag="IW781_001632"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1182633.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20638..20937
                     /locus_tag="IW781_001633"
     CDS             20638..20937
                     /locus_tag="IW781_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1182634.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20987..21499
                     /gene="ssb"
                     /locus_tag="IW781_001634"
     CDS             20987..21499
                     /gene="ssb"
                     /locus_tag="IW781_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1182635.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21525..21761
                     /gene="rpsR"
                     /locus_tag="IW781_001635"
     CDS             21525..21761
                     /gene="rpsR"
                     /locus_tag="IW781_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1182636.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21904..23877
                     /locus_tag="IW781_001636"
     CDS             21904..23877
                     /locus_tag="IW781_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1182637.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23883..24335
                     /gene="rplI"
                     /locus_tag="IW781_001637"
     CDS             23883..24335
                     /gene="rplI"
                     /locus_tag="IW781_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1182638.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24609..25976
                     /gene="dnaB"
                     /locus_tag="IW781_001638"
     CDS             24609..25976
                     /gene="dnaB"
                     /locus_tag="IW781_001638"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1182639.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         8019 a         5219 c         5233 g         7956 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtgtca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagcc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gacttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg
    14521 tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga
    14581 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
    14641 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
    14701 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
    14761 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
    14821 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
    14881 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
    14941 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
    15001 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
    15061 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
    15121 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
    15181 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag atcaggcgga
    15241 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
    15301 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
    15361 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
    15421 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
    15481 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
    15541 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
    15601 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
    15661 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
    15721 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
    15781 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
    15841 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
    15901 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
    15961 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
    16021 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
    16081 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
    16141 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
    16201 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
    16261 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
    16321 ttctggtgga cttcacggag taggatcttc tgttgtaaac gcgctttcta cacagcttga
    16381 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
    16441 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
    16501 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
    16561 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
    16621 agaaccagtc atcaatgaat accattatga cggcgggatc aaaagttatg tcgaatactt
    16681 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
    16741 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
    16801 tgccaacaat atccatacgt atgaaggtgg aacacatgaa tcaggattca agactgcttt
    16861 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
    16921 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
    16981 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
    17041 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
    17101 gcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
    17161 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
    17221 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
    17281 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
    17341 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
    17401 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
    17461 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
    17521 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
    17581 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
    17641 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
    17701 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
    17761 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
    17821 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
    17881 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
    17941 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
    18001 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
    18061 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
    18121 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagacc
    18181 gtgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
    18241 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
    18301 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
    18361 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
    18421 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
    18481 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
    18541 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
    18601 ttatggggaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
    18661 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
    18721 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
    18781 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
    18841 tcattgacat ccgtcgtgac gccagcgcat ccgttatctt gaataactta tataaattga
    18901 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
    18961 ttttgagctt gaaacaaatc cttgaaaact atatcgaaca tcaacgcgaa gtcatcacac
    19021 gacggacggt ctttgaaaaa gaaaaagcag aagcaagagc acacatctta gaaggtttgc
    19081 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
    19141 acgaggcaaa agcaacaatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
    19201 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
    19261 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
    19321 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
    19381 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
    19441 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
    19501 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
    19561 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
    19621 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgtaca gccaaaggaa
    19681 tcccaatcat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
    19741 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
    19801 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
    19861 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
    19921 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
    19981 gtacagcttc cggggtcaga gggatacgcc tgcgtgagga agattatgtc gttggggcag
    20041 ccttaatgaa ggaaaatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
    20101 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
    20161 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
    20221 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
    20281 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
    20341 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
    20401 aaccatcaag actaagtgtg taagctagca agaataaccg attaaaaaat ctggttattc
    20461 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
    20521 ttgcttttga ccaaaataga gtgtataatt agagcttgtg agtagtctaa ttaattagat
    20581 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
    20641 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
    20701 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
    20761 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
    20821 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
    20881 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
    20941 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
    21001 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
    21061 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
    21121 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
    21181 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
    21241 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
    21301 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
    21361 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
    21421 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
    21481 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
    21541 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
    21601 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
    21661 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
    21721 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
    21781 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
    21841 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
    21901 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
    21961 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
    22021 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
    22081 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
    22141 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
    22201 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
    22261 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
    22321 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
    22381 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
    22441 aactatgcag atgccattga taaattagat gacaaagaga tttcttattt gaactctttc
    22501 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
    22561 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
    22621 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
    22681 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
    22741 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
    22801 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
    22861 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
    22921 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
    22981 ctggcacgct tcaaccagaa aaaagcttac atcgttatca atgagaacga gatcattcca
    23041 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
    23101 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
    23161 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
    23221 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatattgaa
    23281 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
    23341 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
    23401 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
    23461 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
    23521 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
    23581 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
    23641 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
    23701 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
    23761 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
    23821 cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
    23881 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
    23941 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
    24001 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
    24061 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
    24121 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
    24181 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
    24241 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
    24301 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
    24361 ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag
    24421 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
    24481 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
    24541 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag
    24601 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    24661 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    24721 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    24781 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    24841 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    24901 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    24961 tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa
    25021 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    25081 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    25141 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    25201 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    25261 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    25321 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    25381 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    25441 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    25501 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    25561 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    25621 tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    25681 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    25741 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    25801 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    25861 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    25921 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    25981 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    26041 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg
    26101 tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag
    26161 caaacgtcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
    26221 gctgatctta cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag
    26281 tcacaatatc atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt
    26341 aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat
    26401 acgcccaatc aaacgaaaaa tatgaga
//
LOCUS       DACEOM010000041        26352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEOM010000041 DACEOM010000000
VERSION     DACEOM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.042"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..1228)
                     /locus_tag="IW781_001639"
     CDS             complement(98..1228)
                     /locus_tag="IW781_001639"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1182640.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IW781_001640"
     CDS             complement(1877..3079)
                     /locus_tag="IW781_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1182641.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IW781_001641"
     CDS             complement(3139..3342)
                     /locus_tag="IW781_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1182642.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IW781_001642"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IW781_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IW781_001643"
     CDS             4879..5691
                     /locus_tag="IW781_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1182643.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="IW781_001644"
     CDS             complement(6715..6867)
                     /locus_tag="IW781_001644"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182644.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IW781_001645"
     CDS             complement(7058..7936)
                     /locus_tag="IW781_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1182645.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="IW781_001646"
     CDS             complement(7975..9093)
                     /locus_tag="IW781_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1182646.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="IW781_001647"
     CDS             complement(9090..11189)
                     /locus_tag="IW781_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1182647.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCKKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="IW781_001648"
     CDS             complement(11240..12061)
                     /locus_tag="IW781_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1182648.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="IW781_001649"
     CDS             complement(12058..12867)
                     /locus_tag="IW781_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1182649.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="IW781_001650"
     CDS             complement(12888..13382)
                     /locus_tag="IW781_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1182650.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="IW781_001651"
     CDS             complement(13394..13882)
                     /locus_tag="IW781_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1182651.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="IW781_001652"
     CDS             complement(13959..16655)
                     /locus_tag="IW781_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1182652.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="IW781_001653"
     CDS             complement(17083..18156)
                     /locus_tag="IW781_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1182653.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="IW781_001654"
     CDS             complement(18153..18980)
                     /locus_tag="IW781_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182654.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="IW781_001655"
     CDS             complement(18981..19787)
                     /locus_tag="IW781_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182655.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="IW781_001656"
     CDS             complement(19799..20884)
                     /locus_tag="IW781_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182656.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="IW781_001657"
     CDS             complement(20901..21443)
                     /locus_tag="IW781_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1182657.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="IW781_001658"
     CDS             21701..22690
                     /locus_tag="IW781_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1182658.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="IW781_001659"
     CDS             22687..23424
                     /locus_tag="IW781_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1182659.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="IW781_001660"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="IW781_001660"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1182660.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="IW781_001661"
     CDS             23988..24620
                     /locus_tag="IW781_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1182661.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="IW781_001662"
     CDS             24705..25352
                     /locus_tag="IW781_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1182662.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="IW781_001663"
     CDS             25357..26340
                     /locus_tag="IW781_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1182663.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8060 a         5570 c         4464 g         8258 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt ttttacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg at
//
LOCUS       DACEOM010000042        25562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEOM010000042 DACEOM010000000
VERSION     DACEOM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.043"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="IW781_001664"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="IW781_001664"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1182664.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="IW781_001665"
     CDS             complement(860..1678)
                     /locus_tag="IW781_001665"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1182665.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IW781_001666"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IW781_001666"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1182666.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="IW781_001667"
     CDS             complement(2967..3923)
                     /locus_tag="IW781_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182667.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="IW781_001668"
     CDS             complement(3923..5056)
                     /locus_tag="IW781_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182668.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQVLCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="IW781_001669"
     CDS             complement(5049..6611)
                     /locus_tag="IW781_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182669.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="IW781_001670"
     CDS             complement(6823..7905)
                     /locus_tag="IW781_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1182670.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="IW781_001671"
     CDS             complement(7971..8369)
                     /locus_tag="IW781_001671"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1182671.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IW781_001672"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IW781_001672"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1182672.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="IW781_001673"
     CDS             complement(9098..10399)
                     /locus_tag="IW781_001673"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1182673.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="IW781_001674"
     CDS             complement(10540..11556)
                     /locus_tag="IW781_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1182674.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="IW781_001675"
     CDS             complement(11864..12469)
                     /locus_tag="IW781_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1182675.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="IW781_001676"
     CDS             12665..13588
                     /locus_tag="IW781_001676"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1182676.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="IW781_001677"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="IW781_001677"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1182677.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15703..17334)
                     /locus_tag="IW781_001678"
     CDS             complement(15703..17334)
                     /locus_tag="IW781_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1182678.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17348..19021)
                     /locus_tag="IW781_001679"
     CDS             complement(17348..19021)
                     /locus_tag="IW781_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1182679.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19034..19351)
                     /locus_tag="IW781_001680"
     CDS             complement(19034..19351)
                     /locus_tag="IW781_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1182680.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IW781_001681"
     CDS             complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IW781_001681"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1182681.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21013..21798
                     /locus_tag="IW781_001682"
     CDS             21013..21798
                     /locus_tag="IW781_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1182682.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21801..22493
                     /locus_tag="IW781_001683"
     CDS             21801..22493
                     /locus_tag="IW781_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1182683.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22767..23138)
                     /locus_tag="IW781_001684"
     CDS             complement(22767..23138)
                     /locus_tag="IW781_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1182684.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23538..23768)
                     /locus_tag="IW781_001685"
     CDS             complement(23538..23768)
                     /locus_tag="IW781_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182685.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23862..24200)
                     /locus_tag="IW781_001686"
     CDS             complement(23862..24200)
                     /locus_tag="IW781_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182686.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(25128..25244)
                     /locus_tag="IW781_001687"
     CDS             complement(25128..25244)
                     /locus_tag="IW781_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1182687.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         7460 a         5609 c         4508 g         7985 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtacttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcaa tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg agggggaatc
    15241 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
    15301 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
    15361 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
    15421 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
    15481 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
    15541 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
    15601 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
    15661 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
    15721 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
    15781 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
    15841 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
    15901 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
    15961 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
    16021 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
    16081 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
    16141 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
    16201 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
    16261 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
    16321 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
    16381 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
    16441 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
    16501 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
    16561 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
    16621 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
    16681 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
    16741 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
    16801 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
    16861 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
    16921 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
    16981 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
    17041 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
    17101 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
    17161 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
    17221 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
    17281 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
    17341 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
    17401 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
    17461 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
    17521 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
    17581 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
    17641 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
    17701 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
    17761 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
    17821 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
    17881 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
    17941 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
    18001 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
    18061 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
    18121 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
    18181 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
    18241 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
    18301 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
    18361 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
    18421 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
    18481 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
    18541 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
    18601 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
    18661 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
    18721 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
    18781 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
    18841 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
    18901 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
    18961 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
    19021 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
    19081 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
    19141 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
    19201 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
    19261 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
    19321 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
    19381 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
    19441 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
    19501 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
    19561 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
    19621 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
    19681 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
    19741 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
    19801 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
    19861 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
    19921 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
    19981 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
    20041 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
    20101 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
    20161 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
    20221 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
    20281 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
    20341 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
    20401 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
    20461 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
    20521 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
    20581 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
    20641 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
    20701 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
    20761 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
    20821 ctgattgcat tataaacgca tccaaaacat ttgtcaacaa aaacaaacac aaataatcat
    20881 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
    20941 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
    21001 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
    21061 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
    21121 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
    21181 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
    21241 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
    21301 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
    21361 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
    21421 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
    21481 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
    21541 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
    21601 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
    21661 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
    21721 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
    21781 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
    21841 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
    21901 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
    21961 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
    22021 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
    22081 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
    22141 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
    22201 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
    22261 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
    22321 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
    22381 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
    22441 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
    22501 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
    22561 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
    22621 tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata
    22681 gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat
    22741 acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc
    22801 tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta
    22861 tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta
    22921 agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct
    22981 ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc tgaagaaata
    23041 atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg ggattgattg
    23101 atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc atactccgca
    23161 aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca accaggagaa
    23221 atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg agtgttgatt
    23281 ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat aagctaatta
    23341 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
    23401 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
    23461 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
    23521 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
    23581 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
    23641 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
    23701 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
    23761 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
    23821 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
    23881 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
    23941 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
    24001 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
    24061 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
    24121 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
    24181 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
    24241 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
    24301 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
    24361 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
    24421 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
    24481 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
    24541 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
    24601 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aatggagtga
    24661 gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata atggttattt
    24721 gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca aaacctttga
    24781 ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca aaggtgttaa
    24841 aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat tgaaaaataa
    24901 ctacagataa tacttttata aacgtacgtg ctttgtggtt ctgtagttaa ctatataact
    24961 ggaattaggg aatggtatca atttgaaaag aaagattcta ccaatttatc atatgttaaa
    25021 aatggaaaat caacagcagt tctttcggaa accttacaca aaaaccgatt attgaaaata
    25081 gccgaaaaac gtttttcggc tattttttta attaagtttc cttcttctta ccttttccca
    25141 ctattccgtt tcacatcttt gatgatcttc agcgcacgtt ttcgtgtttt gatatttggg
    25201 cttttcaagc ggcggtatgc acttgagata tctgtttttt ccatatggac gacccacttt
    25261 cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa caattacgat ttgtgtttta
    25321 aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa cagtcgatca gactagctga
    25381 ttgactccta tctctggaaa taaattttca ttattacgtt ttagctacct aaaaaaggcc
    25441 ctcttgtccg ttaactttaa tatctattgt tatagctagt ttacctattg aacgtactgt
    25501 gtgttcgata gaacactaaa aaagaggctg ggacagaagc gtttaactcc aagaaataag
    25561 aa
//
LOCUS       DACEOM010000043        25430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEOM010000043 DACEOM010000000
VERSION     DACEOM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.044"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(58..855)
                     /locus_tag="IW781_001688"
     CDS             complement(58..855)
                     /locus_tag="IW781_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1182688.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSVPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVIKAISQQLKDVTSIFKLPKAVGKLLGSIQTNLPESVLLDCGMDFL
                     KDNNKKIDTLSVPVDGSWDFNDNTPSGSVLELDLTKNQEAIKKFLNN"
     gene            complement(947..2110)
                     /locus_tag="IW781_001689"
     CDS             complement(947..2110)
                     /locus_tag="IW781_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1182689.1"
                     /translation="MKKRKKIDWWILGPYLTLSMIGLLEVYSASSYRLLQADENTKSL
                     LLRQLIFIFLSWSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGA
                     QRWISLFGIQFQPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPK
                     IAGALMILSIAWVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNH
                     AYERIATLRDSFIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIF
                     SIITEELGLIGALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVG
                     SIAGLMPMTGVPLPFVSYGGTSYLILSLGIGITLNISSKIQAEELPLYRPEKQ"
     gene            complement(2627..2833)
                     /locus_tag="IW781_001690"
     CDS             complement(2627..2833)
                     /locus_tag="IW781_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182690.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2849..3463)
                     /locus_tag="IW781_001691"
     CDS             complement(2849..3463)
                     /locus_tag="IW781_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1182691.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(3610..4293)
                     /locus_tag="IW781_001692"
     CDS             complement(3610..4293)
                     /locus_tag="IW781_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1182692.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RSNTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(4402..4860)
                     /locus_tag="IW781_001693"
     CDS             complement(4402..4860)
                     /locus_tag="IW781_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1182693.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4944..6707)
                     /locus_tag="IW781_001694"
     CDS             complement(4944..6707)
                     /locus_tag="IW781_001694"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1182694.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6928..8388)
                     /locus_tag="IW781_001695"
     CDS             complement(6928..8388)
                     /locus_tag="IW781_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182695.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(8596..9522)
                     /gene="glsA"
                     /locus_tag="IW781_001696"
     CDS             complement(8596..9522)
                     /gene="glsA"
                     /locus_tag="IW781_001696"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1182696.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(9545..10972)
                     /locus_tag="IW781_001697"
     CDS             complement(9545..10972)
                     /locus_tag="IW781_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1182697.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(11003..12217)
                     /locus_tag="IW781_001698"
     CDS             complement(11003..12217)
                     /locus_tag="IW781_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1182698.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(12558..12800)
                     /locus_tag="IW781_001699"
     CDS             complement(12558..12800)
                     /locus_tag="IW781_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1182699.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(12832..13389)
                     /locus_tag="IW781_001700"
     CDS             complement(12832..13389)
                     /locus_tag="IW781_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1182700.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(13402..13590)
                     /locus_tag="IW781_001701"
     CDS             complement(13402..13590)
                     /locus_tag="IW781_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1182701.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(13603..14169)
                     /gene="amaP"
                     /locus_tag="IW781_001702"
     CDS             complement(13603..14169)
                     /gene="amaP"
                     /locus_tag="IW781_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1182702.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            15399..15872
                     /locus_tag="IW781_001703"
     CDS             15399..15872
                     /locus_tag="IW781_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1182703.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            15881..16108
                     /locus_tag="IW781_001704"
     CDS             15881..16108
                     /locus_tag="IW781_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1182704.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            16744..17115
                     /locus_tag="IW781_001705"
     CDS             16744..17115
                     /locus_tag="IW781_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182705.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(17371..17613)
                     /locus_tag="IW781_001706"
     CDS             complement(17371..17613)
                     /locus_tag="IW781_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1182706.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(17645..18547)
                     /locus_tag="IW781_001707"
     CDS             complement(17645..18547)
                     /locus_tag="IW781_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1182707.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(18560..18748)
                     /locus_tag="IW781_001708"
     CDS             complement(18560..18748)
                     /locus_tag="IW781_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1182708.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(18762..19325)
                     /gene="amaP"
                     /locus_tag="IW781_001709"
     CDS             complement(18762..19325)
                     /gene="amaP"
                     /locus_tag="IW781_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1182709.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(19363..20256)
                     /locus_tag="IW781_001710"
     CDS             complement(19363..20256)
                     /locus_tag="IW781_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1182710.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(20334..21272)
                     /locus_tag="IW781_001711"
     CDS             complement(20334..21272)
                     /locus_tag="IW781_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1182711.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(21306..21656)
                     /locus_tag="IW781_001712"
     CDS             complement(21306..21656)
                     /locus_tag="IW781_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1182712.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(21689..22591)
                     /locus_tag="IW781_001713"
     CDS             complement(21689..22591)
                     /locus_tag="IW781_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1182713.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(22584..23441)
                     /locus_tag="IW781_001714"
     CDS             complement(22584..23441)
                     /locus_tag="IW781_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1182714.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            23775..24584
                     /locus_tag="IW781_001715"
     CDS             23775..24584
                     /locus_tag="IW781_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1182715.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            24624..25121
                     /locus_tag="IW781_001716"
     CDS             24624..25121
                     /locus_tag="IW781_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1182716.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         7527 a         5019 c         4206 g         8678 t
ORIGIN      
        1 aaaagaagtc agaacataac atagattgtt atttctaact tcttttattt tctttactta
       61 attattcaga aattttttga ttgcttcttg gtttttggtc aaatctaatt ccagaacact
      121 tccggaaggc gtattgtcgt tgaagtccca actgccgtct actggtacgg ataatgtatc
      181 gatcttttta ttattgtctt tgaggaaatc cataccacaa tccagaagca cactttctgg
      241 aaggttcgtt tggatactac caagtaattt cccaaccgct ttgggtagct tgaatattga
      301 agttacgtct ttcagctgct gagagatagc tttgatcact tgctctcctt tgtttatata
      361 tacgccatct aaatctagag atttttctgc atcgatcttg actccaccag gaaacagttc
      421 attaacgcaa tcgataaaat cttgaaatgt gatacttgca taatagggag cttcaaattt
      481 gaaattttcc tttaatgttt gatttaataa atcaactccc ccataagagt aagctgcatt
      541 tatcttattc attccgtaac ctggaatatc gacatatgaa tctctcatga tagagatcag
      601 ttttgcttgt tgtgttttct ggttgtattg tgcaaccaga agtgaatccg agcgaccgtt
      661 ttcgtctttc gaacgagcat ctgaaccaat caaagccaca tttagtgtgg tagggacact
      721 tttcggctct acctttttca cagttgaact ctgttttgca gacgaaaatc catggataaa
      781 ctcaatcgaa aaatagccaa ctactaatgt aagcagtaac aaaatagata atatgatttt
      841 ttgagatact ttcattgcga attcctcttt ctaaaagaac ctctcgcttg aaaaataaaa
      901 gattgtactt tatgataatt caaactgaga gtttctcagt ttctctttat tgtttttcag
      961 gtcgatagag cggcaactct tcggcctgta tctttgaaga aatattcaac gtgattccga
     1021 ttcctaaaga aaggatcaga taacttgttc ctccgtagct aacaaatggc aatggcacac
     1081 cagtcattgg catcagcccc gcgatacttc ccacgttcat tatcgtctga acgaatagca
     1141 aagttccgaa tcctaaaaga aacaatccag cttgctgatt tttacagcga ctgcttaaac
     1201 aaaagattct catgcataat gagaacaata agaaaagcac acacaatgca ccgattagcc
     1261 caagctcttc tgtaataatc gaaaaaataa aatctgtttc tgtctctgga agataccctt
     1321 tttttgtgat actatttcct aatccccgcc caaatatgcc accattatat agtgcataaa
     1381 acgaatgggt catttgatag cccgcaccat gactatcgat aaaagaatct cttaaagtcg
     1441 cgatcctttc ataagcatga ttaaacatct gtggaagcca acctttattt cctaaatata
     1501 ataccccgcc tgctgctcca atcagcaatg cagaaaaagt aacgattaga tagatccctt
     1561 ttttaaatgg aaccgccgct gcccaaaata tgacccacgc aatcgaaagg atcatcaatg
     1621 ctccagcaat ctttggctga aataaaatca gaaaagttat acctactgta atcaggaatg
     1681 gtttttttag atcttttggg ctactatttc cgtcacgaaa aaaccagctt aaataaaaaa
     1741 tcaaaaaaag atttgccagt tcagaaggct ggaattgaat gccaaacaga gagatccaac
     1801 gttgtgcgcc gttgacagtg acaccgaata tccctattct tactaatact aaaaagaaaa
     1861 tcgataaggc taaaccgtat cctgctattt tagggtgaag taaatagtgt agtttgactg
     1921 aacgagctaa gaagatcacg ctccaactca aaaatatgaa aattagttga cgcaataata
     1981 gactttttgt attttcatcg gcttgcaata aacgataaga actggcactg tatacttcta
     2041 aaagaccgat catggacaaa gtcaaataag gacccaaaat ccaccaatcg atcttttttc
     2101 tttttttcac cgcatctcct cctcacacag caatccactt gtcaggtgag gagcgagttt
     2161 gtctcatcaa ataagtcagt cacaaaaata taaagaaaag gtcagaaacc ttttcgaaca
     2221 agaattctga cctagcatca tctcatcgtc cgagttttag cactatacaa cgtaaagaaa
     2281 acattcttag ctattggaaa aagccttaat ccgacagttt ctgttttacc cattggcgac
     2341 tttcgacgtt tctgggcaac agcctatgtt tgtataggag cctcacctaa caaggttatt
     2401 actatattct atttacgagt tcatgataga cttttcctaa tgattaggca atggaatttg
     2461 aaatgaaata gtgaaattta acaatcttta atatttttta gaaaaaaata acaaataagt
     2521 tgaaagaatc ccgttgcaaa ttctttcagt tgcaccagga ttctttgata attttttagt
     2581 tttatgtgtt tttttatatg tttttttcac ttttcggaat aaatatttat ttgttaaagt
     2641 aattaaacaa tccgctaatc gtttgatctg gcatttctat ttccttacca tctaaagtaa
     2701 cagtagtaac gacaaaatgg tcttgcaatg gcggaacttc taagtactgc ccttgcagaa
     2761 aatcattcac tgtttcatat tcttttttca tttcttttga atctgtcgat ggtttatagc
     2821 ttatttttaa cataactagt gctccttttt attcgggacg tgcaatcgtg aaaatttctc
     2881 ctattgctgc ataatcattt cctgccaaac ggcttacagc attcaatttt cttggatcga
     2941 tccttcctgt cttatcatca tagactgttt catttaaatg atatttctgt acttctagca
     3001 aaaataaatc agctgttact ttatttttat tccgtatctc gacatgttca tataaggatg
     3061 tttcaaaacg aacttttgct tctgtcaatg ctggtactga aatgattact gaatcagtta
     3121 aagtcaagtc agtcaggtac agttcgcttt cctcattagg cagattggct gctgttttat
     3181 ttacttcttc tacgttgtcc tcatcaagga tatggataac agcttctctt gtctcaagaa
     3241 tattctttgc tgtgtctttt actgcatctt gttttctctg gatcgccaag gataagatgg
     3301 gtcgattcga actgacgata ttgaagtaac tgaagggtgc gatattcact atatcttttg
     3361 ctgatttagt cgttactact gctactggtc tagggatgac tgaaccaatc agcaacttgt
     3421 aattttcacg ttctgtcaat tctgaaggac tccgttcaaa catggtttcg ccttctttct
     3481 ataaaattcg ttaaaaagca gatcttgaag acaaaatgaa aagtaatgct tttttcatct
     3541 tcttagaatc tgctttaaac tatctatcaa tttttctttt ttgtcaagaa tctgtgccgt
     3601 gcgtgttttt tagttatttg ctttacttgc ggcttcgaca tatcttttag tgatcaattg
     3661 cggacgggtg ataatagatc caactacgac tgaaaaagca cctaattcat atgctttctc
     3721 tacttgttca ggagagtcag tatgtccttc cataattact gggatatcga cttgttctat
     3781 gatttgattc aataaatcaa aatcaggtgt ctgcgtcttt cgtgaatatt ctgtatagcc
     3841 tgctaaagtc gtagacaaaa gatcaaaacc atcttcttgc gcttgaagcg cttcctcaaa
     3901 agtcgaaata tctgccatag ctaaccgacc gtgttcgtga atcatttgga caagttcagc
     3961 agttgttttt tctgtcggac gtttacgaga agttgcatcc agtgcaatga tttctgcttg
     4021 cgtctccaaa agttcttcca cttctttttc tgtcgcagta ataaacactt ctgacccatc
     4081 gtaatctctt ttgataatcc ctattaccgg taaatcaacg attcgcttga tttcattgat
     4141 atcttctgtc gtatttgatc gaataccaac tgctccgcct tcttttgcag ccaaagccat
     4201 tctccccata ataaaagaac tgtgtagcgg ctcgttttcc aaagcttgac aagaaacgac
     4261 gactccgcct ttgatttgtt ccagtgtatc cacaatatct cctccagtaa tgttatataa
     4321 aaatacagct tcatataaaa atgtaaccct tttctattca ggagtcaata atgagctatt
     4381 tctttcagca gacctttctt actaaaagaa aattacttca aattcttcgt atacgaccag
     4441 cttctcttct gtaaattcta tgttcaacgc ttctttataa gcatgtttaa aaaactcagc
     4501 atggactttc cgccaataag ctaatgtccg atccccttct ccttctttcc aagcatgctc
     4561 tttcgtgact tcactaaaag gcaagatggt cacatttata tttttgatga tacaaactgg
     4621 ttcatttttt ccattcagga ccacgtctaa ttgaccaact tttggtaaag gctcattctc
     4681 ttgtttatac agatcgtaac cacttgatgt accagtcttt atctttttta caactagttc
     4741 gctcaactcg tctgccattt tttctgtatc cccaaatgaa aagaaaccta atttttctga
     4801 ttgataacta ggatattttt cttggaactt tttgacatat tcttctactt gctgatacat
     4861 aacatccacc cctttgcttt ttataatgaa aaaaccatcc agatactagt ataaacaatt
     4921 atctggatgg tgagcataat gaattattga atattcaaga cacgcagact atcttcaacg
     4981 atttgttttg gtgttaaatg ataacgttca taaagctgcc ctacaggaac gctatccgtg
     5041 aattctttct ctgcaccaaa atttaatacg ttcattgaag aatcaccata ataacgactg
     5101 atattttcgc caaatcctcc tgacaaacta ccatcttcta atgtgatgac taattcgtgt
     5161 gtttctttta atttatctaa tgtttctacg tcattttctg tgatcgttcc tggattgatc
     5221 aacgttgctt cgattccttt ttttctaaaa gcttttactg tttcttttcc taattcaaga
     5281 aaaccgccta aagctaaaat tgctactttt cctccattat ttactgtttg ataggctgga
     5341 atactataat catttgcgat agctgtacca tgttctacac catgtgtagg aattcggatt
     5401 gctacaggct gcgctgtttg ttcgaacgcc caatctagca tagagatcat atcctctttt
     5461 gttgctggag caagatattt gatgttcggg atacttgata agtaaggaat gtcaaatgtt
     5521 ccttggtgtg ttcggtcact agctgagatc gttccgccac cgactaaaat aatcactgga
     5581 ttctcgttga tcgctaaatc atgtgataat tgatcatatg cccgttgcaa gaaagtactt
     5641 gattggaaga cgactggacg tactccctga gaagccaaag cagaagcgaa agtgatagag
     5701 tgctgttcag cgatccccac gtcaaaataa tgatctggat gtttttcttg gaatgttttc
     5761 agtccaaagg cgcctggtat cgctgcattg actgcggcaa taggagctgt acttgtttgc
     5821 attttcttat ccaaataatc catgatgatc atattaaatg tttctccaga agttacttgc
     5881 tttgcctccc cggtggacag atcaaatggt acatgccaat gatatttcat tttattatcg
     5941 attgccggct gataaccgcg acctttttcc gtatggacgt gaagtacaat cggatgatca
     6001 atatctttta cttcttcaaa taaacggatc accgtttcca gatcgtttcc attatccacg
     6061 taacgataat caaatcccat tgctttaaat aggttattgc tgcttttacc gttggtttga
     6121 cgaagctcag ctaagttttt atataaactg ccatgattct catcaataga catctcatta
     6181 tcgtttacta atacgattag attggattgt aaaacaccac cattgttcaa tccttcaaaa
     6241 gctaagccac cacttaggga gccgtcaccg ataaatgcta ccacatttcc tgattctttc
     6301 ttcagatctc tcgctttagc taaacctgtt gctaagctga tagatgtaga tgtgtgcccc
     6361 actgtaaaat agtcatgttt actttcatgt tggttggaat atccgctcac ttcacggtaa
     6421 tgtccttttt caaaaccatt ttttcgacca gttaaaagct tatgaccata agactgatgg
     6481 gaaacatccc aaacgatttt atcctttggt gaatcaaata cataatgaaa cgcaatcgtt
     6541 aattctacta cacctagatt cggaccaacg tgcccaccta catcgctgtc cttttgcaag
     6601 attttttggc gcatctcttc tgccaactgt ttcatttcct caacagataa ttttttgaca
     6661 tctgcggggc tattgattgc atccaataca tgtaatactg tactcataat acattctctc
     6721 ctccgtacta tcttctttta atgaaaatag atttgttaca ctttatctat tctactactt
     6781 agagttgact ctaagtcaat agcattttaa aaaaatttca gagaaagctt gttatataag
     6841 tagatcttcg cacgaatcag gactcaaata aaaaccttct acagacaatt aaaaagcttt
     6901 gtagaaggtc tcctaattaa ggtctcatca agaaatatgt ttttttaata ggtgaagttc
     6961 ctgagcaata tgatacaaat ggatgtttgc accttttaat gagattggat agatctttgt
     7021 cttttcagtt tctaatccat tttgatagct agcagcatcg cacaaaacca ctcttttttt
     7081 cgcacataga cattcatcag gcaattcttc taatgaacga aataaaatag gatttatttc
     7141 agcgatgcct tcttccgcat acaaatgaaa ttgggttcga taaaaaaatt gtttgactga
     7201 actttctgaa acaacaaaaa tttctatttc ctgtctcccc gcttgttcac tgttgatcaa
     7261 agaaagactt ttgacgaatt ttcgaatgaa attttcattc catcttagtt ggatttgatt
     7321 tttctttttc cactcttcca atatttctac aatttttccg tacagaagtt gttctttttc
     7381 tgatagaaga tagatccttt ccggtaaaaa tacttgtcca tctgcccaca acgtacggac
     7441 aaattgtaaa aaggattgct tgaaaataaa attttctgca tcgtcgattt cagcgatact
     7501 aataatctgt aaaatcaatt cattcagtga taggtcattg agaacaaaat ttttataaac
     7561 aacttcgaaa tcttttctta acagctccat gttctcattg gtataatttc tactgttgaa
     7621 caaagaaaaa atataataga catcttcttc ttgtaagtct agaattcctt tttgctcttc
     7681 caatccttga cagactaatt gaaataacgg cttcgtttct gcgcttttct tatcttcttc
     7741 tttcagtcca aggggaaatg cctttcttcc gattgcgatc tctattcctc gtatgataaa
     7801 ggacttttca tcttccgaat atcttctttt aaaaaaatga gtctctacat aatcaatcaa
     7861 ctcgttacaa gcttcttcta catacggatt tttattttca tagccttccc aattgacata
     7921 acgtactaaa gctagcaaga tgttccgcga gtcaaattct gggataatga tttctttatt
     7981 tttcaaataa tcattttctt taaaaaaatc caatacgatt tttttgattg tatatacttt
     8041 gctcaaagaa ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata
     8101 ataacttaaa aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag
     8161 agcaatacca tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat
     8221 acaatattct agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac
     8281 ccttagagaa atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca
     8341 cagatacact tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg
     8401 cctccagttt ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt
     8461 tttatttaga aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac
     8521 aagctcacaa tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc
     8581 ctctttcaag aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa
     8641 caattggata ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat
     8701 gccgtaacga tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac
     8761 accaacatgg acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga
     8821 tttgacgatt gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc
     8881 tttatttgcc agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca
     8941 ttgtctgaaa taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt
     9001 catatagtaa gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc
     9061 ttcattcaat gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc
     9121 aaatttttca tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt
     9181 aacaaaagga ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa
     9241 ggcaaaaccg gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt
     9301 gattgctaat aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc
     9361 gtctcctccc tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt
     9421 gggatccact tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg
     9481 gaaatttttt ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca
     9541 ttttttattt ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa
     9601 ctaagacgat aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg
     9661 cgatactagc aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta
     9721 aacaagcaac gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac
     9781 tgtcatcttt aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt
     9841 agattacaac agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta
     9901 atgtaaagac ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag
     9961 gaacaccgtg tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta
    10021 cgtgaagtgc ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac
    10081 aaacaagaat cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata
    10141 ggaattctaa cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag
    10201 tagcgacggt caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca
    10261 ttgctaatgg ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt
    10321 ctacgcccat atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa
    10381 aatcaggaat caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat
    10441 aatggatccc taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga
    10501 tccgagcaat caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga
    10561 aaataatcaa taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg
    10621 ttgcatagga taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct
    10681 caaaccattg gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc
    10741 aagtatagac accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg
    10801 ccacagggat aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg
    10861 aggtcgcgaa tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa
    10921 agccaaagaa cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc
    10981 ctcttttcta tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc
    11041 cttcttcttg ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa
    11101 tcactgttgt cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc
    11161 ctaagaagta ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta
    11221 tcagaatcgt acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat
    11281 cccattgttt ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc
    11341 caatcagcat ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc
    11401 cagcaacgga tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa
    11461 tgatcagcca agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga
    11521 ctgcttgttc atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa
    11581 gcagtcctgt accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag
    11641 gaatctttct ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt
    11701 ggatgactgc tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc
    11761 atatccagtg gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat
    11821 agccttttaa attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga
    11881 taaacatcag ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt
    11941 tggggaagct tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc
    12001 cgatatcccg tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc
    12061 caacggagag aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc
    12121 cgtaaaaaaa agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga
    12181 atgctgtatc tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat
    12241 tacgagtata aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc
    12301 atttataaaa gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca
    12361 cacaaataaa gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct
    12421 ttttttattt ttaaagatat tttttcataa ggaataaaga atacttcata taaaatatat
    12481 aaaaaagaac cgcagcgagt gtcgctacgg ttctcaaatt tgttcttcat ttcaacataa
    12541 gtttttcttc aatttgctta taatttacgt gcaaagaatg aaacgactaa tactaaaatg
    12601 attgcaccga tcaatgatgg aatgatagcc attcctgcaa ttgttggtcc ccaagttcct
    12661 agtaaagctt gtccaattgc tgaacctact aaaccagcaa tgatgtttgc gatgatcccc
    12721 attgatgatc ctttattcgt gattgctcct gcaattgctc caatgatacc gccgacgatt
    12781 aatgcccaaa taaatcccat tttaaattcc tcctaactga cttcttactt cttattgtac
    12841 acgtggttcg ttattttctt tgactttttg tgcacctttg cctacagcag attttgcttt
    12901 atctgtttgg cgtgaagcaa attgtcctgt ggattttgct gcttctgtta ctttgtcttg
    12961 tactgtttct tgatcttttt ggtattcttc ttgtgttttg atgtctgcaa cattcacatt
    13021 cacttcgata acatctaaat gtgtcatatt gtttacttca gtactgatca attctttgat
    13081 ttggttgtaa atatcttcga tgtctttacc gtattctacg atcacatcta gatccacagc
    13141 tacttgtttc ttacctactt ctgtattgat acctgctgtt acgttatctg tgttgactaa
    13201 tttttcagcc atatttgaga aaaatccgcc atctaatgtc aatagaccat ctacatcttt
    13261 caatgcataa ccgatgattt tttggatgac tttgtcttca taagtcaatt caccattcat
    13321 catgcggttt tctccttttt catttgccaa aactgatcca tttgttgtat tcatgttttt
    13381 gttttccatc tttgtttcct cctatgattt aaaaaattga tcaactattc ctgactgttt
    13441 caaataaaaa ccgattcctg cgccaattgc tgtcatgatt atgatcaaca atgtcttgaa
    13501 aaatccaatc gttaaccata aaacagctag cagtagtcca agacccccaa agataatagg
    13561 gagcttgtat gctgaaaata gttctttcat tatttggccc tcctattcca ctcgagagtg
    13621 cttgtagtct cttgtatctt cttgttcttc ataaccgctg tatgtgactg ccactttgac
    13681 cgtttctttt gttccgagta ctttagttac ttcttcttcg atatcttgca tcaatacacc
    13741 tgttttccct actaatgaag aagttcgttt caagtctccg ttgacatgga tatggattcg
    13801 atttttcgtt gaacgtatcc gtactttagg tgaatgaatg aactcttctt cgtgtaaatg
    13861 ggaacgtacc aacccttcga tcgctttttt gtcgattgtt agtttgccat cttcccgttt
    13921 cataacgaaa gttcctcttg actttggata gaaaagaacg actagcaaga tgataatagc
    13981 aagaacggca aatactacag ctgcccagaa aatatattgt tgcatgtagt aattcgttaa
    14041 agtaaagaag cgaaatcgtt ccaattgttc aggcataata tacggcgcat tgatcaacgc
    14101 aatcgctaga aatactgaaa gtagcagtaa actcatgata acaccgatta tttttaaccc
    14161 cttattcatt ttccacctcc tataaactta tttttaccct gtaaataact ctaccgacaa
    14221 tcacagtata gatagcgaac agttagcata gcatcagtaa ataaagcgct accaaacatt
    14281 gctggatcat aacggcgttt tattgccagt ctgatactga aataacaatt gcttacttat
    14341 tctctattta cttttgattg ctggctatac ttaaaggata ttattcaaga ttaaaaattg
    14401 caaagattat gctcagttat tattacaaga ttctatttat ttattaaatt ttttcggaga
    14461 attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt atttttatat
    14521 tatcattttt cttattcact tctttggttt atactattct tataaataaa aatgaggtat
    14581 gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat ttgattgtat
    14641 caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact actacattat
    14701 gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga aaaaaataaa
    14761 catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt attcggcaat
    14821 ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat aaactaatcc
    14881 aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc catgcgtcaa
    14941 tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata agtggtgatt
    15001 acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac ttgaaaaaat
    15061 taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct gttaacttac
    15121 acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat aatcccaggc
    15181 atctaaacta gcattataga agagacacac atagaatcta aaataactgg ttttaaatgt
    15241 ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct atcatttttt
    15301 ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa taattattct
    15361 gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa acattgctat
    15421 taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta cttttttata
    15481 ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca ttatttgttt
    15541 ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta ttgaaactat
    15601 tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata ctaaaaaaaa
    15661 tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct aaatttatga
    15721 aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct gtcgtattca
    15781 ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa catgcgattt
    15841 tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta tcgttaattt
    15901 agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga aagtgggcat
    15961 tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc gtttttccac
    16021 tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga cctataaaaa
    16081 tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa ataaaaaata
    16141 atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat tcagattttt
    16201 tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca tacaaagggg
    16261 gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct gttacgttgt
    16321 agttggatct gcttttagta taatacttta ttcattccac cagactgata gcagtttgag
    16381 cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga agtgcttaaa
    16441 acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt aagctcccat
    16501 atatactgta aagccttcta tatattatgt catgttgctt tttaacattt actatgtagt
    16561 tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt aacattattt
    16621 tgataataat gattatttat ttaaaaatac attagataac atttgttacg acacgaaaaa
    16681 gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat taggaggaat
    16741 agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat tccattacca
    16801 acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc tgctcttacg
    16861 aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta ttggctaaaa
    16921 cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct ctttgagttt
    16981 ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat catctgggtt
    17041 ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa atttagggag
    17101 gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag gaaaaacttt
    17161 taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa gtcttgtcac
    17221 agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat atgcccaacc
    17281 aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga tgaagaacag
    17341 ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga atgatacgat
    17401 tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg caatacttgg
    17461 tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg ctacgacatt
    17521 tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg ctccgataat
    17581 tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat gttttacttc
    17641 cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg cttccagctg
    17701 ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc gctgcgtcag
    17761 ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt ttgatgtcca
    17821 ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact tctttgccga
    17881 ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc acaatgacat
    17941 ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc gttacattat
    18001 ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca gataataaac
    18061 cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct tcataattca
    18121 gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta tttgtatgat
    18181 cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct tgtgctttcg
    18241 catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt gcatcatgtt
    18301 gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt tctgcatcat
    18361 gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga tgtgcagata
    18421 ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt gtcgttgggt
    18481 ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt ttgttattgt
    18541 tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc gtttgtttca
    18601 gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag gtcttgaaaa
    18661 agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag atgataggga
    18721 gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac tcgtttgcgg
    18781 tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac tttcactggc
    18841 tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa tgttcctgtt
    18901 ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc gatcttgttt
    18961 ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg caagtgagaa
    19021 cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc atgtttcagt
    19081 agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat aattgtcaaa
    19141 atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt agtgatggaa
    19201 aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa tagtatcgca
    19261 aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt tacaccttta
    19321 ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt tgatcgcttc
    19381 tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg atgctaagaa
    19441 aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac tttgctgacc
    19501 aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg tccagattgg
    19561 accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg ctaaaccgat
    19621 cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt cgcttggatt
    19681 gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat gcggaacagc
    19741 ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt tgacttgctc
    19801 aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta gtaccatcgt
    19861 atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg cttgttcgtc
    19921 acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt attttgctac
    19981 ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg ccccttctct
    20041 agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga ttagtacacg
    20101 gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag gaacaggaat
    20161 catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct ccgtatggta
    20221 caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc tagtttttaa
    20281 attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat tctttaattt
    20341 gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa agagccaaag
    20401 actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt attcattccc
    20461 caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc tagatatttc
    20521 atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat actctctaat
    20581 aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg gacatcatag
    20641 agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa ttgatccatt
    20701 gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc aagatctgcc
    20761 tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa agcgtcaatt
    20821 cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa taatgtatac
    20881 gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag ccgatccaca
    20941 aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac aaaagatatt
    21001 ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac taccaaagaa
    21061 aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc ttccggatat
    21121 tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg ctcaatctct
    21181 tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt ttctattaca
    21241 ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg actttctcga
    21301 aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac tgcgatttca
    21361 ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg tttaccgaaa
    21421 taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat tttagtcgca
    21481 tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt tgcaaaagca
    21541 cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat ttttgtattt
    21601 tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat ttccattcct
    21661 actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt tttggttgaa
    21721 cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc gtcatccctg
    21781 catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac ggtttcacat
    21841 gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca cgttcacctg
    21901 ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc caacgcggat
    21961 gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca tggaatcgta
    22021 atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt tcatctaacc
    22081 attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat ttagctgaaa
    22141 ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct acgattgaat
    22201 caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg tcgcaatcca
    22261 tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca atacgataat
    22321 aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct ttattgattt
    22381 tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt aaaagctgtt
    22441 tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta gacgggttgc
    22501 aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt gtgttacaac
    22561 ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt tagagtactg
    22621 tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt attccaaggt
    22681 ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt atactgacgt
    22741 tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg catgttccat
    22801 tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc ttggtcgtga
    22861 tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt aatttctttt
    22921 ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa gtcatcggaa
    22981 tcgattccta atctttccaa agctttcact tggcctggat cgataatagc gccgattgtt
    23041 tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa cacatctgaa
    23101 tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa agaaattctt
    23161 aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat atctttttcc
    23221 actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc ttccaatcct
    23281 tctttactat attctgatag accatcgtca atcacataaa attttatgcg tcttgtttta
    23341 ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata attttcgtct
    23401 gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac tcctttttct
    23461 aatctttttc taacctgttc tacatctaga atattcctca tttggtttta aagcaaaata
    23521 tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca tcggtaagcg
    23581 ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac tactatatat
    23641 agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag caagttagtt
    23701 tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat gtaaaaagaa
    23761 agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg cttgatcgaa
    23821 atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa aatggcggaa
    23881 tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt tccgaattag
    23941 gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg aaggatggaa
    24001 aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga aaagccttct
    24061 tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa gatgatcagc
    24121 tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat ttcaatttac
    24181 agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt agctatctta
    24241 aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga gatctgcaag
    24301 cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg cctacaggta
    24361 tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa tcgagtgaca
    24421 tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc gggttgagta
    24481 ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta acactgacga
    24541 atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa taaaatgacg
    24601 atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat catattcttg
    24661 atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt attgctcaaa
    24721 gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat ataccagttg
    24781 aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt ctttctccac
    24841 aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa actttagctg
    24901 aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa gtagtcggga
    24961 ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct tttcctgaag
    25021 cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa tccgctttag
    25081 agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta tcttcattta
    25141 cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac gtgctttttc
    25201 tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca cgacctcttt
    25261 cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc aaaatatgag
    25321 gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt cacaacctct
    25381 tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
//
LOCUS       DACEOM010000044        25214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEOM010000044 DACEOM010000000
VERSION     DACEOM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.045"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..403
                     /locus_tag="IW781_001717"
     CDS             5..403
                     /locus_tag="IW781_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1182717.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IW781_001718"
     CDS             387..1082
                     /locus_tag="IW781_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1182718.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="IW781_001719"
     CDS             complement(1676..2563)
                     /locus_tag="IW781_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1182719.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="IW781_001720"
     CDS             2748..3587
                     /locus_tag="IW781_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1182720.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="IW781_001721"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="IW781_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1182721.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="IW781_001722"
     CDS             5055..5411
                     /locus_tag="IW781_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182722.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5414..6571
                     /locus_tag="IW781_001723"
     CDS             5414..6571
                     /locus_tag="IW781_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182723.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="IW781_001724"
     CDS             complement(6597..7037)
                     /locus_tag="IW781_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1182724.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="IW781_001725"
     CDS             7197..7703
                     /locus_tag="IW781_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1182725.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9784
                     /locus_tag="IW781_001726"
     CDS             7871..9784
                     /locus_tag="IW781_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1182726.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9789..10790
                     /locus_tag="IW781_001727"
     CDS             9789..10790
                     /locus_tag="IW781_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1182727.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10992..11198)
                     /locus_tag="IW781_001728"
     CDS             complement(10992..11198)
                     /locus_tag="IW781_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1182728.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11844..12692)
                     /locus_tag="IW781_001729"
     CDS             complement(11844..12692)
                     /locus_tag="IW781_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1182729.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12856..13758
                     /gene="gnd"
                     /locus_tag="IW781_001730"
     CDS             12856..13758
                     /gene="gnd"
                     /locus_tag="IW781_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1182730.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13760..14761
                     /locus_tag="IW781_001731"
     CDS             13760..14761
                     /locus_tag="IW781_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1182731.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14758..15042
                     /locus_tag="IW781_001732"
     CDS             14758..15042
                     /locus_tag="IW781_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1182732.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15075..15872
                     /locus_tag="IW781_001733"
     CDS             15075..15872
                     /locus_tag="IW781_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1182733.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15886..16719
                     /locus_tag="IW781_001734"
     CDS             15886..16719
                     /locus_tag="IW781_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1182734.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16737..17594
                     /locus_tag="IW781_001735"
     CDS             16737..17594
                     /locus_tag="IW781_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1182735.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17616..18125
                     /locus_tag="IW781_001736"
     CDS             17616..18125
                     /locus_tag="IW781_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1182736.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18162..18563
                     /locus_tag="IW781_001737"
     CDS             18162..18563
                     /locus_tag="IW781_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182737.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18675..19028
                     /locus_tag="IW781_001738"
     CDS             18675..19028
                     /locus_tag="IW781_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182738.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19062..19505
                     /locus_tag="IW781_001739"
     CDS             19062..19505
                     /locus_tag="IW781_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1182739.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19522..20607
                     /gene="uxuA"
                     /locus_tag="IW781_001740"
     CDS             19522..20607
                     /gene="uxuA"
                     /locus_tag="IW781_001740"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1182740.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20600..21313
                     /locus_tag="IW781_001741"
     CDS             20600..21313
                     /locus_tag="IW781_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1182741.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22065..22265
                     /locus_tag="IW781_001742"
     CDS             22065..22265
                     /locus_tag="IW781_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1182742.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            22833..23396
                     /locus_tag="IW781_001743"
     CDS             22833..23396
                     /locus_tag="IW781_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182743.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(23457..23822)
                     /locus_tag="IW781_001744"
     CDS             complement(23457..23822)
                     /locus_tag="IW781_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182744.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(23898..24143)
                     /locus_tag="IW781_001745"
     CDS             complement(23898..24143)
                     /locus_tag="IW781_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1182745.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(24213..24467)
                     /locus_tag="IW781_001746"
     CDS             complement(24213..24467)
                     /locus_tag="IW781_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182746.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(24631..24954)
                     /locus_tag="IW781_001747"
     CDS             complement(24631..24954)
                     /locus_tag="IW781_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182747.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            25141..25214
                     /locus_tag="IW781_001748"
     tRNA            25141..25214
                     /locus_tag="IW781_001748"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25175..25177,aa:Ile,seq:cat)
BASE COUNT         8763 a         3880 c         5137 g         7434 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta
     9721 ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg
     9781 gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta
     9841 ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta
     9901 gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc
     9961 aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata
    10021 ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga
    10081 tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc
    10141 tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa
    10201 aacatcctac accagttatc caattgaacg aagccattca agcagctaaa aaagagataa
    10261 aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta
    10321 gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc
    10381 cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt
    10441 ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg
    10501 cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca
    10561 gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta
    10621 cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag
    10681 tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata
    10741 aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga
    10801 tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac
    10861 aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg
    10921 gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta
    10981 aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct
    11041 gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat
    11101 attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca
    11161 ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata
    11221 attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa
    11281 aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc
    11341 aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa
    11401 tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct
    11461 aaactttttt attttacata acaatcatat caactgtgaa ataaaattac aaatgataac
    11521 ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt
    11581 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
    11641 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
    11701 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
    11761 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
    11821 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
    11881 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
    11941 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
    12001 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
    12061 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
    12121 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
    12181 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
    12241 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
    12301 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
    12361 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
    12421 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
    12481 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
    12541 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
    12601 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
    12661 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
    12721 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
    12781 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
    12841 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
    12901 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
    12961 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
    13021 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
    13081 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
    13141 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
    13201 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
    13261 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
    13321 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
    13381 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
    13441 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
    13501 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
    13561 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
    13621 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
    13681 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
    13741 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
    13801 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
    13861 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
    13921 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
    13981 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
    14041 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
    14101 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
    14161 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
    14221 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
    14281 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
    14341 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
    14401 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
    14461 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
    14521 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
    14581 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
    14641 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
    14701 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
    14761 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
    14821 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
    14881 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
    14941 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
    15001 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
    15061 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
    15121 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
    15181 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
    15241 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
    15301 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
    15361 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
    15421 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
    15481 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgcgg
    15541 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
    15601 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
    15661 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
    15721 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
    15781 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
    15841 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
    15901 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
    15961 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
    16021 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
    16081 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
    16141 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
    16201 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
    16261 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
    16321 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
    16381 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
    16441 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
    16501 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
    16561 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
    16621 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
    16681 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
    16741 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
    16801 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
    16861 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
    16921 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
    16981 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
    17041 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
    17101 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
    17161 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
    17221 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
    17281 gccgtaatgc tacaggccta atgcaaaaca ctggtgttca gacagcgatt gaaatgctat
    17341 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
    17401 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
    17461 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
    17521 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
    17581 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
    17641 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
    17701 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
    17761 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
    17821 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
    17881 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
    17941 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
    18001 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
    18061 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
    18121 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
    18181 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
    18241 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
    18301 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
    18361 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
    18421 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
    18481 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
    18541 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
    18601 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
    18661 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
    18721 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
    18781 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
    18841 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
    18901 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
    18961 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
    19021 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
    19081 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
    19141 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
    19201 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
    19261 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
    19321 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
    19381 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
    19441 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
    19501 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
    19561 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
    19621 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
    19681 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
    19741 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
    19801 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
    19861 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
    19921 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
    19981 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
    20041 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
    20101 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
    20161 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
    20221 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
    20281 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
    20341 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
    20401 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
    20461 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
    20521 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
    20581 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
    20641 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
    20701 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
    20761 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
    20821 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
    20881 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
    20941 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
    21001 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
    21061 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
    21121 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
    21181 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
    21241 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
    21301 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
    21361 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
    21421 gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa
    21481 gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc
    21541 ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa
    21601 aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata
    21661 gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat
    21721 aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt
    21781 aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg
    21841 gttattcaac tttttcttcg attcgaagac aatagaagac aatgaatttt atatttcatt
    21901 gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa
    21961 cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg
    22021 caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag
    22081 taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg
    22141 tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag
    22201 cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag
    22261 cttaataaaa attgactttt aaccgtcttt tcgaaagaaa agacggtttt tttgtacgaa
    22321 aaaattcatt ggtcgttgaa tgggctgaat agtagtgtat aaaacaattt tgtggagcgt
    22381 gaagctttta tggtagagga aaatacgaat aaagtgatgt tgataaaaag attcctatct
    22441 tgaatatgaa tcgagatgtc ttgaaaatga atgagtttga ccaatcatta tcctatcgaa
    22501 atttacagtt tcgttcgatt ggtctgtcaa ttaatgaaaa agtaaaaaag aatatgttct
    22561 aggaagaggg cggcacatct aaatttttaa gtaacggaca gttcaaatat caagcattga
    22621 aagaggctat cctactgttt gaaggaatac gaatatataa aaacgacaaa gaggaaaaag
    22681 tagttcatta aatcccttca agtgatgaaa gaatgagatc taataaaacc tgatctttca
    22741 tctgtttttt taataacgta ataatttttt tattttttgt gctttcgccg ttattgtaga
    22801 gtgatttagc attcttattt ttaaaaataa ctgtgttata tattaaaaaa ggcataatag
    22861 ttataagctt ttttaaagaa tatttagaaa ttattaattt tcgtctgaaa acaaaaaaag
    22921 aaagcgttat tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt
    22981 tactatcaat attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac
    23041 tgatcacaaa atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg
    23101 actacagagg cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc
    23161 gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa
    23221 gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg
    23281 tcaggagatg ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt
    23341 ttagttttgc acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta
    23401 agatggttaa agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag
    23461 gtaagataaa ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg
    23521 tgttccggat aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat
    23581 gatttgccac gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc
    23641 atttcatcca tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca
    23701 taatccacag tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga
    23761 tgttgtccta aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc
    23821 atagaaatgt ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc
    23881 accaaggctg gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa
    23941 tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct
    24001 tctccttctt cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct
    24061 acttgagata aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt
    24121 aaaatagctt cttcgatact catttaatca cctttcatta tagtagaata gttataatga
    24181 ctatagaaag ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa
    24241 taaagaatga cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta
    24301 tcgggttcaa agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc
    24361 aaaatagcta aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc
    24421 agtgcgaccg caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct
    24481 caacgattca tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg
    24541 aaaataaaag aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa
    24601 gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc
    24661 taagtaattc aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat
    24721 gatcccatag cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct
    24781 ttttccttta tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat
    24841 cattccaata aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc
    24901 cgcaactaaa tcgaataaaa tcgcaataaa tggcaaaata atcactccta acattgagaa
    24961 taaataaaag aataacattt tttctaaaat aagcaaataa aaattaaagt aacaaaatag
    25021 ttatgacaca agtaaaagaa ataagaataa caatattatt ttacttatat tttgaaactt
    25081 gtgagagttt gtcattcgtg ctatagttga aaagtaacaa aaataaattt tatttatgat
    25141 gtacctttag ctcagttggt tagagcagac ggctcataac cgtccggtcg taggttcgag
    25201 tcctacaagg taca
//
LOCUS       DACEOM010000045        24778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEOM010000045 DACEOM010000000
VERSION     DACEOM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.046"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            125..724
                     /locus_tag="IW781_001749"
     CDS             125..724
                     /locus_tag="IW781_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182748.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            836..1060
                     /locus_tag="IW781_001750"
     CDS             836..1060
                     /locus_tag="IW781_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182749.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            1412..1648
                     /locus_tag="IW781_001751"
     CDS             1412..1648
                     /locus_tag="IW781_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182750.1"
                     /translation="MLSDYKEELKLIENRVLGFVECKDNLDDTLEVLESIIFNTKVLD
                     LKAINGDMTTHIILDEISAKEIGETLIAWANKEL"
     gene            1728..2582
                     /locus_tag="IW781_001752"
     CDS             1728..2582
                     /locus_tag="IW781_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1182751.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            2595..3032
                     /locus_tag="IW781_001753"
     CDS             2595..3032
                     /locus_tag="IW781_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182752.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            3352..3795
                     /locus_tag="IW781_001754"
     CDS             3352..3795
                     /locus_tag="IW781_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1182753.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            3940..4263
                     /locus_tag="IW781_001755"
     CDS             3940..4263
                     /locus_tag="IW781_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182754.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            4681..8310
                     /locus_tag="IW781_001756"
     CDS             4681..8310
                     /locus_tag="IW781_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182755.1"
                     /translation="MKKLKLISIALFSLITMSTARQSIVSAEGTSTKAVVEESGDKKV
                     AIDDKVKNEAEKATVVQGADGLMYVVVDENDPEKLLEAESPLKNQQENTGAVEVGVDE
                     KRQEKADKANEKVKNFDDIDYNDISSLQSALNDPKFPFLSEDELNEKLQKTKNTFESE
                     ALMKKTRGYFSQRILELSNNLNDVPKRAKVSRHALVSNRASQVSETRINPPNGYPWVI
                     YLIRSNGYSYTENYAIRRTIDGKEAFCLQAGVQATNTYTGSTSGYLSNSQREMISSIL
                     SAYKELGGWANNDAGKQRWIATQMLVWENSNEKPKSITVTPNWKNGATKWKNDLVARA
                     NAIRVRTKWDSTSKTVDEGDKATFKVTNTYGDHNLHVKSVSGGGKATISNGTLTVDTT
                     GATGNKITVDMIKGSHPSDAHPTTVWSNGSYQKLVTGSFYISTSVTVNVKRKGTVEVL
                     KVDSETKKPLSGATFRFSYSGKTKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQ
                     LDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDGLGLPNVVFTIYNSDNKVVK
                     DNLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEISQDTPFTKPAKVKVT
                     NIYEVSISEKVSDMNESQVLKNNWSADPNDYLQYDITTGNLKKSGERNVQSFIIEGFY
                     DNKNVDVSKSEVYVGSTNVTNSFDINNDTNNGKVIAKAKTSVLTNNDFYNKSYNLRIT
                     MKIKKSTLENTEKQKNLTIVKNSKVTVGSKISKSASSNDVETVLYSRKVTINHIDEKD
                     KHLLKQDIDYKYDGETYEYKPRTDLFDKDGNNYKSDVIHKGKIDGKDIVLNTPYHIPV
                     LSVNVDRIQIDTARAVKNGYLPTKIELSKKEEYEKELEKIVFKVKITDIDNNKVVYDE
                     NFKYKEFNSHMSLDLPTEYLTKNKKINYSVDILLVDNPDKNKFETETKDLRTIGYTSS
                     EKVLTNKDMTNNQVNYSAVVRTVKERENNNPVKEFNESINYKFTDSTKTKTGYGISVD
                     LEPTYRNEIGKQIDMKVYSYPMNELIDSKIDEIYQSGGKETEVKMDRLKNDGKVESGI
                     STQNLSFQFPHMNIERKTGNIYADSQRNDESIKYELIDGGRKLYVPIWCDLGNYSIAI
                     KSSNFGGNYISLDMNQNIDVYAYMYATIDSETKKDDELLLEPVYPESTLPDGWSKNEI
                     DWLNK"
     gene            8370..8732
                     /locus_tag="IW781_001757"
     CDS             8370..8732
                     /locus_tag="IW781_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182756.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            8966..9388
                     /locus_tag="IW781_001758"
     CDS             8966..9388
                     /locus_tag="IW781_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182757.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            9400..10815
                     /gene="cpaF"
                     /locus_tag="IW781_001759"
     CDS             9400..10815
                     /gene="cpaF"
                     /locus_tag="IW781_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1182758.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            10832..11647
                     /locus_tag="IW781_001760"
     CDS             10832..11647
                     /locus_tag="IW781_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182759.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            11647..12444
                     /locus_tag="IW781_001761"
     CDS             11647..12444
                     /locus_tag="IW781_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182760.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            12462..12824
                     /locus_tag="IW781_001762"
     CDS             12462..12824
                     /locus_tag="IW781_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HAQ1182761.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            12996..13217
                     /locus_tag="IW781_001763"
     CDS             12996..13217
                     /locus_tag="IW781_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182762.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
     gene            13236..13511
                     /locus_tag="IW781_001764"
     CDS             13236..13511
                     /locus_tag="IW781_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAQ1182763.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            13620..15446
                     /locus_tag="IW781_001765"
     CDS             13620..15446
                     /locus_tag="IW781_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182764.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSIGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELVVSSEETEEVEEIETVSENEEETEEIFDFEDEEEVENVQKGDLS
                     TSSQNGLEAVNDPQVPPYISSQTPNYSPQYQAPNVLQPLRKHETVQLVTLKEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDIAWKKPYDLDVKKNKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            15459..16175
                     /locus_tag="IW781_001766"
     CDS             15459..16175
                     /locus_tag="IW781_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182765.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKG
                     HYENINAQISCPLDRNGETTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     TKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEIKSFDKRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            16188..19046
                     /locus_tag="IW781_001767"
     CDS             16188..19046
                     /locus_tag="IW781_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1182766.1"
                     /translation="MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVI
                     QSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLD
                     TDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMP
                     YRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPI
                     SEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKE
                     AIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQIMIL
                     EQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI"
     gene            19097..21733
                     /locus_tag="IW781_001768"
     CDS             19097..21733
                     /locus_tag="IW781_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182767.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTMISSGIQ
                     FAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNALKEKNF
                     FSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFSDSFKG
                     GYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDIETGQRS
                     KVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVAYVCAVFVLIKG
                     SGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGNGNGSDSGEGED
                     RKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDMAND
                     TKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDRKDGAKVENADR
                     IMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKSAEELINQKRQE
                     AKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKNSNTGLEKLVKENLQNS
                     TSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIGVRENLQSSSTSQ
                     PKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGFKTVD
                     VRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFT
                     YEDNELFRDTLNDPNPLFDNLN"
     gene            21757..22092
                     /locus_tag="IW781_001769"
     CDS             21757..22092
                     /locus_tag="IW781_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182768.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            22089..22706
                     /locus_tag="IW781_001770"
     CDS             22089..22706
                     /locus_tag="IW781_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182769.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            22722..23189
                     /locus_tag="IW781_001771"
     CDS             22722..23189
                     /locus_tag="IW781_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182770.1"
                     /translation="MLKHCRKGFCKVIKGKNAADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEEDSNSKSAFFSEKNAFLHSI"
     gene            23205..>24778
                     /locus_tag="IW781_001772"
     CDS             23205..>24778
                     /locus_tag="IW781_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ1182771.1"
                     /translation="MIVSKKLARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDEC"
BASE COUNT         9366 a         3120 c         4809 g         7483 t
ORIGIN      
        1 ataaaattac ataaacgtga atatattttt atgaattaaa tgtaaaatga gagcaattag
       61 ttttaaaaaa gattataggc tgttaatctt agcatatata atccattgga aagggggaga
      121 tccaatgaat ctaaagaata caaactcaaa caaatctaca ctatatgtaa tttcagatga
      181 ttatataaat aagctgaaaa aggtggacta tcgtgtccaa aacaactatc gagaacaacg
      241 attatatttt aaatcaaact tgaaaatttc taacaaaaat gaagaaataa attattatgt
      301 accattggct tcagcaaaag aaaatcaacg ttctttgact aataaatcag tgtttaagat
      361 ttatggtgat cgagaagaaa aggacttttt aggagtactt catataaata atatgattcc
      421 tgtgccaaaa gattgtgcaa aagtatggac acctaatagc ccaaatgttg atgagaagta
      481 taagatgtta gtaatcaaac aggcaaaata tttgaaatta catgagtctg aaataaatga
      541 atcatgcgaa ttactttata attctaagtt aggtaaactt gatcctagtt actttaagaa
      601 aaatcgtaaa gaagtaatta cttatcaaaa aataatgacg aatgtagaat cattggaagc
      661 tgttagtata gcagaatccc aaagaaatat tcatgtacaa actcaaagta tagatgtaga
      721 gtaagatagg ctaaacctat cttattttat tattctattg gatattatga tacattgaat
      781 tttaatcgaa tatgtattta aatgttatta tactgtagaa gataaaactt aaaggatgat
      841 ttctatggcg agaccgagaa aatacaatac agaagaagaa agaaaagaag caatgaaaaa
      901 ggctaggaaa aaatgggttg aaaaaaatca ggaaactatt gcctatataa gagccaaatc
      961 atttgctaaa aaattcattg aaatgtctga agatgaagat attgaattgt tggaaaaatg
     1021 gttagaagat cggaaaaata acggtcaaaa tgatgaataa taaccattga ctacaagtag
     1081 tcaatggtgt agaatggttt tagggataga aatatccctt ttctaaaagt ctaatgacta
     1141 caagtagtcg atagagggat cgggaataaa aacagacgat tatggcctaa agaaaggagg
     1201 aatatgaata ttttgaggaa cgatttatta aatgagcgtt gataaatttc actaaatatt
     1261 ttcttaatat ctatgtattg ttcaagcgta gaaaaaaata ttgaaattat tacaaaaccg
     1321 ctcataatgg aaaataacga attatattaa tgaatcgttc acttttgacc ttttttacta
     1381 gatttttttg taggactttt agagaatttt tttgttatct gattataagg aggagctaaa
     1441 gttgatagag aatagagtac tggggttcgt agagtgtaaa gataatttag acgatacttt
     1501 agaagtcctt gaaagtatta tatttaacac caaagttctg gatttgaaag caattaatgg
     1561 tgatatgaca acacatatca tattagatga aattagcgca aaagagattg gcgaaacttt
     1621 aatagcatgg gctaataaag agctgtaaaa gataatttta aaataaagtg gccaattgac
     1681 tacaagtagt ctgtatagta taataaatta tcaaaagtga ggtatacatg atgaaatcaa
     1741 attttttggg atatgtaatt gcaatcaaca tgtttaaagg gggagttggt aagtcaactc
     1801 ttactcagct aatggctgat attctatcgg ctagaggctt taaagtttta gttattgatg
     1861 ttgatccaca aggaacagta actaaaaagt ttcaaagaca atatgattta aaagttgttg
     1921 ggaaaatgac attgtttgaa gcagtaatta atatgtcttt cttagactgc atttctcctg
     1981 ttaaagaaaa cttagatatt gtgcttggtt cagaacaaat gtcaaggttt accaaagtat
     2041 taagagatat gtggagaagt actggtttta ctgatgaagg tgaagaagtc ttttggaatt
     2101 tgtttaatta tatgatcggt aatgataatt taagagatca atatgatttt attttgtttg
     2161 acactattcc tacagtttca gaatttacag ataacgttta cgtggcgagt gattttttag
     2221 taattcccac gcaaactgag caagattcta tagacaactt gcatagtacg gttactaatt
     2281 atcaaactgc tgtaaaagaa gtaaacggag atttagaaat tttaggtatt gttccatatc
     2341 tagtggatac taaattaaaa acaggattag aacttttatc agaacttcaa gaagaatatg
     2401 gagacttagt ttattctcat attgtttcag aacgaggcgt aattaaacgt tggggtagag
     2461 aaggactaaa taaatctatc ccacattcta aaactgctta tagcatgtat gtagacattg
     2521 tagaagaaac gttagtacgt ttagaaaaag ctacaggaga aaatatttta gaacgtgttt
     2581 aggaaaggtg ttgcatgatg tcgaaaacta aaaaagtaac tagtgccggc agtaatggtt
     2641 cgggtaatcc gttcggaata aaaagcggta gtggtggatt taatatagga caacaaatgt
     2701 taaaaggtaa agctggcatt aagtctactg gtgataaacg gaaatctgtt agggtaccag
     2761 ttgaattata cgatgaagtt gttaaagatt tatcatacgg ggctaaatat tcattaatga
     2821 cagaaatttt agctttattt ttaaaagaat ttgaaacaca tggaaatagt atagttactg
     2881 attttggcaa gttgaaaacg agaccagtaa aaatttctga agaagccttt aaagttttga
     2941 aagttataaa atttgaaaca gatcaatcta atactaatat tattgcttca gctttgacat
     3001 ggtatcttga taaaagaaaa ggaaaagcat aatgaaatga ggtaagacta tggatcataa
     3061 atcaaattat tcatacaaag aaaattatat ttggaagtat gtagtagccg tttttctact
     3121 ggtaggtgga attattgcat tggttaaatg ggctataatt cctaatgctt tagtatttat
     3181 caattggttt gtccctaatt tttggagttt ttttggctat atattgataa tctggtttat
     3241 gtttaaagta ctctcttttt tctggtcgcc tgtatattct ttgcttggaa agtggtcgta
     3301 cctaatttta atagcgacag ctatttatat cattatgcag attaactaat tatgcagatt
     3361 ttagaagata ttaaaattta ttggaaaagg ataataggct atacattagt tcttcttgta
     3421 gtaaatttat tcctttcgat gatagcgttg gtctttgtta atcaaagaaa agacaatgta
     3481 actgaattag taacgtttat ttcgtttttc ataagtggga tttttgctcc tttctttgaa
     3541 gaaaccttaa tgagaggaat tatccaaaac caattggaat taaaaacaaa tttgtctaca
     3601 agaagcatat atataattgt tgcaattttg tttagtctat cccacttaaa tttatatttt
     3661 attccttatt ttatgacgag tttaattttg tcttttgtct atgataattc agataaaagg
     3721 ctgattgttc cggttactat tcattgtttt tacaatttaa ttgtaatact gttaagtgta
     3781 gttttttata cttgaaaaaa atagacgaaa acctaaaaat acaaggtttt tatctatttt
     3841 ttttattttg tttttatcta tcaggttgat agaaattgtc ataaatgata taattaagta
     3901 tgaactgatt tagtaaaaaa attaaaggag tgatagctgt tggcaagatc aagaattgct
     3961 aaaattgaac agactaaaaa ggataaaaaa atttctaaaa ataaaacaaa attctttaaa
     4021 gctgaagatg ttaagaggat agcatggtct gtgaaaacat ttgatgaaga tcgttatgtt
     4081 aaagtttcaa tgaatggtct atttgtacag cttggtaact tcagtaattt taaaacgatt
     4141 gatgaacttt atccggaaat aagaaatatt gtggatagta aaggtttaat cgtggctaaa
     4201 cggaaatcaa gaaactctgg tcttgcttac tgtaaaaata aagggatcat gattccggct
     4261 taaatcagtg aaattgttct ttgaaaactt aatactactc gtactgtagc aatgttgcta
     4321 tgttacagat aaaagcgtaa ggctaataag caaaatagca accaagcata ctgttatttc
     4381 ctttgccaag aggtatgtaa atacgttagc aatagaagtg ataaggttag tatttcacgc
     4441 caaaaaatac aaaagtcgat ccattgaaac tactaattat gaggtggcgt aaatggtaat
     4501 tattcagaat ttctatctag gaagaaccta tgaaaaccgt cctagagatc agaatattga
     4561 cactaaaaaa ttcagcacag taataaatgt gctagataca gggtaactct ttactacaag
     4621 aaatgtagta aagagttgtt atgattcttt attacatttc tttctaaagg aagtgtgata
     4681 atgaaaaaac taaaactgat tagcattgct ctattcagcc taatcactat gtcaacggct
     4741 cgccaatcaa ttgtttcggc tgaaggaacg tctacgaaag cagtagtaga agagtctggc
     4801 gataaaaaag ttgctataga cgacaaagtg aaaaacgaag cggaaaaagc gactgtggtt
     4861 caaggggcag acggcttgat gtatgtggtc gttgatgaaa atgatcctga aaaattgcta
     4921 gaagctgaat ctcctttgaa aaatcaacaa gaaaataccg gcgctgttga agtcggtgtt
     4981 gatgaaaaga gacaagaaaa ggctgataaa gctaacgaaa aagtgaaaaa ctttgacgat
     5041 attgattaca acgatattag ttcgttgcag tcagcgttga atgatccgaa attccctttt
     5101 ttatcggaag atgaattaaa tgaaaaattg caaaagacaa agaatacatt tgagtctgaa
     5161 gcgctaatga aaaagacaag gggatatttc agtcaaagga ttttagagtt aagcaataat
     5221 ttaaatgacg taccgaaaag agcgaaagtg agtcggcatg ctttagttag taaccgtgct
     5281 tcacaagtaa gtgaaacacg aataaaccct ccaaatggtt atccgtgggt gatttattta
     5341 attaggagca atggttatag ttatacagaa aattatgcta ttagaagaac tattgatggg
     5401 aaagaagctt tttgtttaca ggccggtgta caagcgacga atacatacac tggttcaaca
     5461 agtggttatc tttctaattc gcaaagagaa atgattagtt cgattttatc agcatataaa
     5521 gaattaggcg gttgggctaa taatgatgcc ggaaaacaaa gatggatagc aactcaaatg
     5581 cttgtttggg aaaacagtaa tgaaaagcct aaaagtataa ctgttacgcc aaattggaaa
     5641 aacggagcta ctaagtggaa aaatgattta gtggctcgtg caaatgctat tagagtaaga
     5701 acaaaatggg attctacgtc taagacggtt gatgagggag ataaagcaac ctttaaagta
     5761 actaatactt atggagatca taatttgcat gtgaaatctg tatctggtgg cggcaaagct
     5821 accataagca atggtacttt aactgttgat actacaggag ccactggaaa caaaatcact
     5881 gtagatatga ttaaaggttc tcacccttca gacgcgcacc ctacaacggt atggagtaat
     5941 ggttcttatc aaaagcttgt taccggttct ttttacatta gtacttctgt tacggtaaat
     6001 gttaagagaa aaggaactgt tgaagtttta aaagttgatt cagaaacaaa aaaaccatta
     6061 tcaggagcaa catttagatt tagctactca ggtaaaacaa aagatgttac tactgattcg
     6121 aatggtaaag caaaattaac tgaaaggttg aaagctggaa ctgtagtaaa agttaaggag
     6181 ataaaagctc ctaatggcta ccaactagat tcaagcgaat tttcaattac agttaaagaa
     6241 aatcaaaatg ttactaccac aagaaccaat aagaaatcca caggttctgt agaaattgaa
     6301 aagataggcg atttagatgg attaggacta ccgaatgtag tattcacgat ttataattcc
     6361 gataacaaag ttgtaaaaga taacttaaaa acagatagta atgggaaaat aaaagtcgat
     6421 gaactgcaat ttggtaaata ttatgcagta gaaaaacaag gtgttactgg ttatgatccc
     6481 gatggtaaaa aatacaattt tgaaataagt caagatactc cttttacaaa accggctaaa
     6541 gtgaaagtaa caaatattta tgaagtatcc ataagcgaaa aagtatcgga catgaatgaa
     6601 agtcaggtat taaaaaataa ttggtctgct gatccaaacg attatcttca atatgacatt
     6661 acaactggaa atctaaaaaa atcgggagaa agaaacgtcc aaagtttcat tatcgaaggg
     6721 ttttacgaca ataagaatgt agatgtgtca aaaagtgaag tatatgttgg atcaacaaat
     6781 gtaacaaact cttttgacat taataacgat actaacaatg gaaaagtaat tgctaaggca
     6841 aagacaagcg tattaacgaa taatgatttt tataataagt cctataattt acggatcact
     6901 atgaaaatta aaaaatctac tcttgaaaac actgaaaagc aaaaaaattt aacgattgtt
     6961 aaaaatagta aagttactgt cggttcaaaa atctctaaat ctgctagctc taatgatgta
     7021 gaaaccgttt tatattcaag aaaagttact atcaatcata ttgatgaaaa agacaaacac
     7081 cttttgaaac aagatattga ttataaatac gatggggaaa cgtatgaata taaacctaga
     7141 accgatttat ttgataaaga tggaaacaat tataaatctg atgtaatcca taaagggaaa
     7201 attgatggaa aagatatagt tttaaacact ccctatcata ttccggtact ttctgtgaat
     7261 gtggatcgga ttcaaattga tacggctaga gctgttaaaa atggctattt acctacaaaa
     7321 atagaactaa gtaaaaaaga agaatatgaa aaagaattag aaaaaattgt attcaaagtt
     7381 aaaattacag atatagataa caataaagtt gtttatgatg aaaactttaa atacaaagaa
     7441 tttaatagtc atatgtcgct tgatttacct acagaatatt taacaaagaa taaaaaaatc
     7501 aattatagtg ttgatattct tttagtggat aatccagata aaaacaagtt cgaaactgaa
     7561 actaaagatt taagaacgat cggttatact tcttctgaaa aagttttaac aaacaaagat
     7621 atgacaaata accaagtcaa ctattcagca gtagttagaa cagtgaaaga aagagagaat
     7681 aataatccgg tcaaagagtt caatgagagt atcaattata aatttactga tagcactaaa
     7741 acaaaaacag gatatggtat atcagttgat ttagagccaa catatagaaa tgaaattggg
     7801 aaacaaatag atatgaaagt ctatagttat ccgatgaatg aattgattga cagcaaaatt
     7861 gacgaaatct atcaaagtgg gggaaaagaa acagaagtta agatggatcg cttaaagaat
     7921 gatggtaaag ttgagtcggg tattagtact caaaatcttt cattccaatt tccacatatg
     7981 aatatcgaaa gaaaaactgg aaatatatat gctgacagtc aaagaaatga tgaatctatt
     8041 aagtatgaat tgatcgatgg cggtagaaaa ttatatgtgc ctatttggtg tgatttggga
     8101 aattattcta ttgctatcaa aagtagtaac tttggtggta actatatctc gttggatatg
     8161 aaccagaaca tagatgttta tgcttatatg tatgcaacga tagatagtga aacaaaaaaa
     8221 gatgatgaat tactgcttga acctgtttat ccagagtcta ctctaccaga tggttggagc
     8281 aaaaatgaga ttgattggct gaataaataa gtttgaaaag tttttatcta tctgatagaa
     8341 ttaaataatg atatagggat ggtgatagaa tgagggttca aagctatggt aacagtgtct
     8401 ttgatgttaa aagagaattt caagattcta gaagaaaaac caaaaagctg atagcttact
     8461 tcattctatc agctatcgtt ttctatattg tcaagtttgt tttggaatta aactcaatag
     8521 tattcacaga tatggttcat aaaatgttaa atggagaacc tattaatttc aactttgatt
     8581 acatgcttga aaaagcacaa agcaatcaaa ttgtggcttt tatcgtgagt atctattatt
     8641 ttattgaaaa atctattttt tctttaaaag attttttctt tgaaatgctt accaaactaa
     8701 caacgtttta caataaaatt tcaaaaaatt aaggaggaag tttgaatgtt agatcaaaat
     8761 acagacagaa gttggtggat gattggtgcc gtgatcgtag gtgccatctt aatcgggaca
     8821 gctaaagtag cttttcccga agtgtttaat atcgtcatta catttttcaa aaacatgatc
     8881 cctacaaact taggatagtt ccactaaaaa attcacgtag tttgatgcgt agaaaaataa
     8941 taaagaatga aaaaggctac ataaagtgat ttatgtagcc ttttttttgg atggaagtga
     9001 tgtaatgcta agagatatgc ccaaaatgat tcttttaatg tttgttgggt tctttttaat
     9061 tttagtgatt gcgtactatt cattaaatgg ctatacacaa gattcgtctg tgaaaggtat
     9121 gcaagaaacg ttaagaacta cagcaatagc aaacagggat gatagtattc gagtagaaaa
     9181 aggacgattt gctttaatta agtctgattt tgaaaaagat tttaaagaaa aatttgggaa
     9241 aagtaaaaat ctaaataatg aaaccgctga ttataaattt gattatctta cagatgggaa
     9301 ggacggtatt aaagcaatta aagtgaaggt tgaaagtgga tcaaaagaat atcaagcaac
     9361 ttgtatttta aatgttgcaa atgattagag gtgtgaaatt tgcgtgttgt agaggaaaag
     9421 aagatttctc caaaaatcga ggaaactcct aaaagtaaaa ttaatatcgc aagtattatt
     9481 aaaaaaaatg gaattaaaga aaactctgaa gcgatagaag gaaaccttga ttttttaact
     9541 aagcttttag aagaagatag tggaaattat gaaccaccaa ttgaagatgc aaaattaaat
     9601 atgattcgtg atgatttaac cagaaactat tcccaagaag ttttacaagc tttaactgat
     9661 gatgaaatta ggcgaaaact tgtacaagtt gttttgaaag aacatggtac tttctttaaa
     9721 aaagataaag aggttgcaga atatgtagta cgtgaatgta ttggaacagg gattattgag
     9781 cgaattatga ccaacgatga aattacagat gttggttgga atggctctca acttagcatt
     9841 gaaactaact ctaacaaatg gttgtttgac ggtgataaat taaatatcac gcaagagtat
     9901 attgaacgtg ttatttctaa atatgctgtt gtaaatgatc gggcttttaa tagtggtaat
     9961 cctatcatgg atggaatgtt taaaaatgtt cgtatatcag ctacacacat agataattca
    10021 ccagatggct ctacgttttc tatgcgtgtg gcaaggccta gattagcatt gaataaagaa
    10081 aactttgaag attttgctcc tgattatatt ttggagctat ttgagaaaat gatgaaaaca
    10141 aaagcaaata tctgtatatc aggggagaca ggtacaggta aaacggaact tcaaaaatta
    10201 ctgctaagtt ttattgataa tcatgatcgt atgattatga ttgaggacgt tcaagaaact
    10261 cacgctaaag aattatttcc ggataaagat attttctctt ggatcactac tcccaaaaaa
    10321 gcaatttcag atttagtaaa agcttctctt agaaataatc cacgatggat cattgtttca
    10381 gaaacacgtg gttctgaagc ttatgaaatg cttcaagcgg ttttgtcagg tcactatgtt
    10441 gtaacgacac ttcatgcggt taatgcacgt gcgataccta gacgttttgt aaatatgtgt
    10501 gcaagtggtt ttaaaataaa cgaaaatgcg gttgaggaag acataaatcg ttatttcgat
    10561 tttggtgtcc atatcaaaaa aacagtggtt gatggtaaag tgatccgtta tttgaatgaa
    10621 attgttgaat tttctgctag tgaaccgact acagttttta agcaaaaatt tatcaatgga
    10681 aagtttgtca ttgaaaatgg tagtctttcc gaagatttta aagaaaaaat ggctgaaaaa
    10741 tttattacgt ttgaatttcc tgaagatcaa actaatacag aagaatcact tcctgaagaa
    10801 gaagaggtga tttaagttga aagtacaggc tatgacaaag cgaaaaggtc tcatggatgt
    10861 ttcggaacta aacgcctatc aagtagcgta tggtaaaccg ttagattata gagattattt
    10921 gaactatgtt ggattacctt gtttgtttat gggtggcttt tcttttgctt tatgttacta
    10981 ctggtggatt gctttgattt gtggagtaat tggaggcgta tatggcttta aagttataat
    11041 gcctaaaagt attaaacgct cttatgaatt aaagagttta caggaaagaa ataaatttat
    11101 taacaacatg actcaaattt tgaccgatga tagtaaaaca gttactagag cgttatctat
    11161 agcaaaacag cgaacaaaag gtgaattaag agaagatatt caaatcttag aatcacgttt
    11221 acaaggtgct gataaatttc agattgctga agcttttcgt gaaatcaaca ataaatatta
    11281 tagtgatgtt gtttttacac aatactttga gcaattggaa acagctattt acgaaggtag
    11341 aaataattca gatactatga agcaaataaa gagctatcat aatgatgtga aacgtaaaac
    11401 agaaggtttt atgaaaatca aagacggcta tcttcaagat atgaagcaaa tgatgtttat
    11461 tgtagctgtt tttattggag ctataacttt tgcttttggt ttttctactt tctatgaagg
    11521 ttttgctcga tcacctatcg gatgggtatt tggatctatt tactttttga ttatggttag
    11581 cttcatgaag tcatttttca aacattattt tgatgatgaa ataatgagtt tgggggtaag
    11641 taattaatga aagttaaggc tttaaaaaag aaaagaaaat caagtattag ccccttgaaa
    11701 attacgaaaa cagaacataa aagcttttgg attaaattac taggtgatgt ttattatcaa
    11761 aaattacgtg aaatgaacaa ttccaatatt gcgattatta agtggcaaaa acaacggtta
    11821 aaaaagtcat tgatttttat cctagtcgga ttcttaatgt atctattttt agggaaagaa
    11881 tggtattact tagccggtgg aattgctact tcagtaggtt tttatgtttg gcaagcaaaa
    11941 tcaatcaatg gtatctataa tcaatttcgg tttgaacgac acttgcaatt tagtaaattt
    12001 acacggctat tgatccccta tctgaaacaa aaaaaagatg gcggaaattt atatggtgtt
    12061 tttgcaaaaa tcgtaaaaag actagattat gataccgata aaaagctatt aatgaagctt
    12121 atgcaagaaa tgacagatca gcctaacgat attagacctt ttacggaata tgcagaacag
    12181 acttctggaa cagacatgtc cgtattattt atgtctacag tttatgatat tcgacaaggt
    12241 tcggcagatt tggatgttat tgaagagttg gatcgtttag cttctgaaga gttaatggcc
    12301 ggaatcgatt caatcattga ctataagtcg aggcgttttg tcttctttcc tacaaaaatt
    12361 gcaatgactt catttatttt agttgtcggt tttgcagtag gcgttctttt catgaaccta
    12421 aaagatatta actttgggca ataaaggaaa ggagaaagtc aatgcaagaa caatccgtta
    12481 tggaaatcat taaatggttt ataggtttaa tgatgattat ggcaatggtt tctataggac
    12541 ttttttgtgt tcaattacaa gatattaata ctttcaagca acaagtaaat tatcaaattg
    12601 aacgaaaagg aggtctgacg acagaagctg ttgaagcaat cgacaaatat tcggctacta
    12661 attatgatag acgttttaca attgaaagcg ataagttaaa tcagaaagta tcttatggag
    12721 aagaaattga ttatacgatc aaagcaacta ttccaattca aattttaccg cttcctgatg
    12781 taactttatc gtttaacgga aatagtgttt ctcaaataag atgagaggaa ggataaaatg
    12841 caagagaata atgtttccag aataatttat ttgattggcg gattgataat tttatcctta
    12901 gctattgccg gtcttgtaga gcattggccg gaaataaagt tattaataag aaacttttac
    12961 gacaataaaa tgattaaaat gtaggaggta tagatttgga agcaattcaa gaaaatcgta
    13021 aggataatca agaaacaagt gaaaaaaaca aaaaaaaaag aaaaaagaaa gtagataaat
    13081 ttacggaaat gaccgataaa atcattgaaa agaaaggact taaacctaaa gactttgacc
    13141 gtcaagcaat caaagaagca cgacaaaaac tgtatgatga ttttgttaaa gaaaacatat
    13201 cacagctttt agactaacat ttagggggaa ataaaatgaa aaatttagtt atcagcaagt
    13261 ttgcaatgtt accaagaatg gattttttcg ataatgtaag taagtttatg tcaaatatga
    13321 cttcaaaact acaaggaatt tcactatctg taattatctt ttgtattgtg gttactggtt
    13381 tgatgtttct tttcggagaa gggccaagtc gtactgctaa aaaatggtta acttatatcg
    13441 tgattggggc aataattatc ttcggagctt caactatcgg acaaacgatc aagtccgttg
    13501 gtggtttcta aggggaagct tcttagaact aaaaaattcg caagtattgc atgaaagagc
    13561 attgagtgat taatgctctt ttttattttg aaaaactata aactaaggag atttaaacta
    13621 tgaaccaacg attcgaatat aattttgaag atgtagaacc taagttactg aaaaaaggaa
    13681 aaggattaaa aaagaaaaat aaatatactc tcaaacaagc agaagcatta gtaggatgga
    13741 ttcaacacaa ttggtcagac aagacaacaa ttaccttgat ctgttcagac acagaagaag
    13801 gaacaaagta tgcttttgat ggaattgaaa ttactccatt ttcgattggc ggacaagaaa
    13861 aattattgtt tcctgatatt gaaggacaaa ttttacaagc gccggacagc atgatttccg
    13921 atcctaataa actgattttg gatattaacg atcaatacaa acacgaacca gacgtagcag
    13981 aaccaacaaa agaatcaaag tcaaaattaa atttgaagct cccttcagta gtattaccat
    14041 ttgctaagaa gaaaaataaa cttatgaacg aattgattga accagaagaa acggatattt
    14101 atgaagaaga attagaagaa caagaagaga tcgaagaatt agttgtgtct tctgaagaaa
    14161 cagaagaagt agaagaaatt gaaactgttt ctgaaaatga agaagaaaca gaagaaatct
    14221 ttgactttga agatgaagaa gaagtggaaa atgtacagaa aggcgatcta tctacaagta
    14281 gtcaaaatgg gttggaagct gtaaatgatc cacaagttcc accatatata tcttcacaaa
    14341 ctcccaatta ctctccacag tatcaagcgc ctaatgtctt acaaccatta agaaaacacg
    14401 aaacagtgca gttagttacg cttaaagagt actgtgattt gggagaagaa attgaaactt
    14461 caatttctca aatggtcgaa aagttgaaac cagaatcttt attcaaattc atagggattc
    14521 cttctacagg tcttacaaat actcgtattg atgaatatag aagtaaccat gctatgttgc
    14581 gattagctga agtaaaattt gaaaatttaa gagagcatta ttcacggcaa attatttctt
    14641 ttaaagcaga agccctagat cgtttaagaa ctgcggtaga tattgcttgg aagaagcctt
    14701 acgatttaga tgtgaagaaa aacaaggccg aagagttatc aaaaatggaa gcagaagcag
    14761 aagctaaatt aaatgagttc gtgaaacgtc aggaaaacaa tgtaaatgat aaaatcaaaa
    14821 agtttgagat cgaacaagaa atagatttaa aaaactttat tgcaagacaa gaggcagata
    14881 aagcggtatt tattgctaca gagaaagatc ggactcaaaa tttaattgat tctaaagcgg
    14941 aatcaatcaa tgcagattta cttgaaacaa aagataaatt tattgatgaa gaaatgtaca
    15001 aaatgaaaat tgaaatgaac aaaaatttat ttgatggcaa gcaaaaaatt aaatcagaat
    15061 tggctactga actcgctaag gcaaatgata atgtttggga aaatgcgctg aaatttattc
    15121 aagaaattca agatgaaatt gaagctaaaa caccaatttg ggctgctgaa atcaaggagt
    15181 ttaatagttt agaagaacaa gaacatgaac gtatgaagca aactaaagaa cttcaattaa
    15241 aagaggaaag tattgaaatc aagcgtttgg aagctgaaat gaatttgaag cgtatggaag
    15301 aattagatca tgaaaataaa atgcttaaaa ttaaacttga aactgccctt tcaaaaaatg
    15361 agttattagc tatggaacaa gatacaaagc aagtggtgaa tggatcaaac agagttggtg
    15421 cacttaattt atttagtaaa cgttaaagga gaacggatat gagtgttttt aataataaag
    15481 ttatgttagg gaactgttat gtgaaccaca ttggttttta cgatggagga acatacagag
    15541 ttttattctt acgtttatct gtttttagtt actcaaaaga tggaaagggg cattatgaaa
    15601 atattaatgc acaaatttct tgtcctttag atagaaatgg ggaaaccacg actacaagta
    15661 gtcaccctaa agccttgatg aataaattta tctttgaaaa gttggatcgc aatttggttg
    15721 aggggatgaa gataagtatt catggaaata ttcgtggaac agaacaagtg cttcttaaca
    15781 gtggttggaa aaactttggt caattatcgc ctttagaaaa agaagaatat caaaaaattc
    15841 ctaaaaatga attagtaaca agaaatcaaa catatatcta tatagaagag gtaagtatgc
    15901 cagaaaaaga agaatttaga aagtctcaat ctaaggtaac aaaagaagat gtggaagttg
    15961 cagaaccgaa tcaagcagat gaaattttat ccgatgatac tgatccagtg aatgatgaag
    16021 gagaggttat tgaagttttt gaagctgaaa ttatcgaaga ggaagaagaa attaaaagtt
    16081 ttgataaaag aatgtatcga gagaaaccaa aaggttatgt gaattttgaa gatatgtatt
    16141 atgacgaata ttctaatgaa ccaccgatca attagtgggg gtcaaatatg aagctagtac
    16201 aaaacaatga agtgaaagaa ataaaaaacg gctttgttaa aaactcgaac aaaattttgg
    16261 gaaagctgaa aaaagtaggt agtttcaaaa aaaataaata tcatactgga aaaattgagg
    16321 gagaagattt accaaaaagt ttacaagata aattgaatgt tccgcttgta tttataggtg
    16381 tttttttagg ttcaatagtt tttctagcaa taacgtactt attgaacttt ttaaaagctt
    16441 tgttagttgc aatcggacag tcagaaagtg ggctacttgg tgtagagttc gatcctaagt
    16501 ttagtttggc atatctaaaa ccacttgggg atacgaaaca atactttatg tgtgctgtgg
    16561 ttgcggtaat aggagtcgct tatttaatat atgggaaatt aaatcatgaa agtgagaaaa
    16621 atcttgttta cggtcaaaaa ggagattcaa gactttcaac tatcaaagaa attaagagag
    16681 attaccatga gataccagaa aaaacagaag tgtatgatgg tattggtggt gtgccaatta
    16741 gtcactatca agataaatat tatattgata gagacacagt aaataccgtt gttttgggag
    16801 cttcacgatc cggtaaaggg gaaacatttg ttgttccttt attagataat cttagccgag
    16861 caaaaataca aagtaatatg gtcgtgaatg atccgaaagg agaattgttt agcgcttcaa
    16921 aggaaacact tgaaaaacga ggttatcgtg tattagtgct aaatattgat gatccacttg
    16981 aatcaatgag ttttaatcct ttacaactag taattgatag ttgggctaac aaggactatc
    17041 acgaagctag taagcgagcg aatactttaa cttcaatgct ctttgcttca ggaatgggta
    17101 cagataatga gtttttctat aaatcagcta aatcagcggt caatgcgatt attttaacga
    17161 ttgtagaaca ctgttttaac aatgattgca ttgaaaaaat tacaatgtac aacgtggctc
    17221 aaatgctgaa cgaattagga agcttatttt acactgatcc aaagactgga aaagaaaaaa
    17281 atgctttaga tgaatatttc aatactttac cacaagggaa tcaggccaag attcagtatg
    17341 gttctacttc atttgccggt gataaagcca aaggttcgat tttatctact gcttcacaag
    17401 gtattgaaat gtttacaagt gatttgttcg ggaagttaac aagtaaaatg tctattgatt
    17461 tgaaagagat aggctttcct aagtccatac aatttaaagt caatacgaat ttagttggaa
    17521 agaggattag tatttcattt ttgagaaaag aaaatgatgt aattagatta attaagaagt
    17581 atcgagtgaa agtaaaagct ttagggctat gtgttttaaa ttttaatgaa tatcttcagg
    17641 atggagatat gttggatata cgatatgaag aagagaaatc gagagcatgg tatcgtattg
    17701 aatttcccaa aaaacaagca aatagtcatg atacttcttt agtcactaaa aaattcaagt
    17761 caggtaaaaa ttattctgac ttagaaatca gcaccttacg attaaaatac acggatcagc
    17821 caactgctgt gttcatggtt attccagatt atgatccgtc aaatcacgta cttgcaagta
    17881 tttttatcag tcaactttat acagaattag caagtaattg taagcaaaca ccggataaaa
    17941 aatgttttag gcgagttcat ttcttgttag atgaatttgg gaatatgccg gctatagaca
    18001 atatggatgg gattatgacg gtatgtctag gaagaaatat gctttttgac ttagtgattc
    18061 aatcctatag tcagctagaa acaagatatg ataaagcttt taaaacaatt aaagaaaatt
    18121 gtcagaatca tattttaatc atgtcgaacg atgaggaaac gatagaagag atttcaaaaa
    18181 aatgtggtca taagacaaca ataaatagaa gtgctagttc taaacattta gatactgata
    18241 gctcggtcac tagttcggca gagcaagagc gagtaattac agttgagcgt gctagtcaat
    18301 tgattgaagg ggaacaaatt attttaagaa accttcatag acaagataca aaaagaaata
    18361 agattagacc atttcctatt tttaatacta aagaaacaaa tatgccgtat agataccaat
    18421 ttttagcaga agactttgat actcaaagag atattaatga gattgatatt gcatgtgaac
    18481 atatcaatct atctttacaa gataaccaaa ttccatatgc aaggtttatc aaagatttaa
    18541 aaacacgttt ggagtattca attattaata atattcctat tagtgaagaa gactatcagg
    18601 attatctaaa tttatcagga tcacaacaaa caattaatga agaagagctg ttaaaactgg
    18661 taaatacgga agtaaaaagt caagatatgg aaggtcaagt tttatcagaa caagaaatga
    18721 aaattaatat ttatctaaag gaaagtcaaa aggaagcaat agaattagta aaagtaatta
    18781 atggaaggtt agctgatgaa aattcagcag atatggttat aaaaaccatt aattcacaaa
    18841 tccttgatac gcctttcatt caatctttaa tcaatgattc tatagctatt cataatgttc
    18901 aagagagtaa ggatcaaatt atgatcctag aacaacaatt agctaatgct tatgagttag
    18961 cagtgtttaa aacgaaaaat tcaatactga aaaataaaat tatcaagttg aaaaatgcta
    19021 tgactaagct agcaaaagca atttagagag aagggaagaa atattgactg aatctattaa
    19081 ttggagaaaa ctaaatgtgc aatggttaaa gtataaatgg ccaatcatag tttccctaat
    19141 aatcattcta tttatggcct atccgcaacg agtttttgcg ttgccgtgga ttacagacaa
    19201 taaagaggta ttaaaatttt taacaaaata tcaagattat cttcagttta ctaaaggtgg
    19261 ttttggaagc attgtcgttc aaagtttatc gtgggggctg attagaggtg catattttgt
    19321 ttgtaaatca gcagaaaacc ttgttccaca atcgctaagt ttgtttagct ttgttgaaag
    19381 cagtagttta ggtgctgttt atcaatctgt attaaacacg gtggttgtag gattaatgat
    19441 cctttcattg atgtttgttg gttacaagat gattactgga aaaggaacag tggatttaaa
    19501 atcggttgga atgaacgtag tgatgtctat agcattaata cttttaatgc caaccatgat
    19561 ttctagtggt attcaatttg ctaaaacatt ttataaggac tctactacga ttacaaaagc
    19621 tgatgggggc gttgcttggt cattgattga ggacggtgta actgatttag tatatatcaa
    19681 taaaaacgat gcttatgatc ggttgggtaa agatggcgta caaaaaaatg cgttaaaaga
    19741 aaagaatttc ttttcctacg atttcagtga agtaatcaca gttgatacag ctaaagattt
    19801 agaagatgat aataaaaaag ctgaatatct tcaatctagg ttgacaccag atggcgaagg
    19861 aaatatgcaa gccgttaaga ttgatgatgg ttggatgtcg atgttctctg atagtttcaa
    19921 aggagggtac tacaggttta ctaaaaatac ttttgcattg actttaggat tactttcgtt
    19981 agctgtagcc tatgttttta gtatttttat catagtcacc gctattttag aactggcatt
    20041 taagcgtgtg ttaggggttc ttgtttttgc aacagatatt gagacaggac agcgttcaaa
    20101 agttgttcta tcagatattc tacagtgcta tttgactgta ggctttcaag gatttggatt
    20161 atccatgttt gcaatgtttg tttcctattt aggaacaggc ggagggtctt ccgttaatcc
    20221 gtttattaag gctgtagctt atgtatgtgc cgtttttgtt ttaatcaaag gcagtggaac
    20281 agtcatgaga tactttggtg ttgatattgg actcaaagaa ggctatgggc aacttgcttc
    20341 tgcttttgga atgggtgcaa tgctgtttag aaaaggatca aatggattta atcgtgccaa
    20401 aggaagcgga aacggaaatg gatcagacag cggagaagga gaagatcgaa aaccagaaaa
    20461 gaatttcggt gaaactctat caaaaaaagc tggcaaaact ggaagagctt tgggatatgc
    20521 ccatgaacgt ggattatcag gtttagcttc agatggagct acaatggcta gtgaaagagc
    20581 aacaaaacca tttaagagta tgcgtgatat ggctaatgat actaagaata agttcaaaga
    20641 aggtttagat gatggtacag tgtccgctat taataaaaat agcaaaccta tgttagctaa
    20701 aaataaagaa gatgaaactg gaaaatacgc tgattccatg ccaacacgtc tttcggatag
    20761 aaaagatggt gctaaggttg aaaatgccga tagaattatg tctagttctg aacgtatgcg
    20821 tgaagcaatg aaaaataatg ctgaatcgaa taataatcct gtaagtgcaa ttcagcaaaa
    20881 agttcaacaa gatattgaag aacgtaaaaa tgctatgcac ggtcaagcta agtctgctga
    20941 agaattaatt aatcagaagc gacaagaagc taaatacacg cctgaagcaa tgaatcgtga
    21001 ggaaatgtta aggaaacgtg tagaagggcg tacaggaact aatacggacg aaaaagaagc
    21061 gttgaccaaa gaaagaatcc aagaagctaa aaactcaaat accggattag aaaaattggt
    21121 caaagaaaat ctacaaaatt ctacaagtag tcaaggtggt cgatcagtag atgtccgaga
    21181 aaatattcaa ggttcattga atggtcatgt cggacggaat gttgatgtaa gagagaattt
    21241 acaaagttct tcaaatggtc aaggtactaa gacaattggt gttcgagaaa atctgcaaag
    21301 tagcagtaca agtcaaccaa aaacagtaga tgttcgagaa aatgtacaaa attctacaag
    21361 tagtcaaggg tttaaatcag tggatgttcg agagaatgtg caaggtagca gtgcaagtca
    21421 accaaaaaca gtagatgtcc gagaaaatgt acaaagttct acaagtagtc aaggattcaa
    21481 aacagtagat gttcgggaga acgttcaaaa agatagtgga gaaatgaaag aaaaaacaca
    21541 aaaaatcaat atcgttgaag acagaaagtc agcgaaaaaa tttgattcaa atcatgaaac
    21601 agtgattatt gatagtgaaa tccgtgaaaa tgataaggga tcaaaaccaa gacgaagatt
    21661 cacttatgaa gacaacgaat tattcagaga tacacttaat gatcctaatc cacttttcga
    21721 taatttaaat taaattaaaa gccggcatga gtgattatgc cggcttttta tgaggtgaaa
    21781 atgatgtata gaaatcccaa aaatataaaa caagagataa agctattttt cttctattta
    21841 gtcgatttag gaatagtagc cggaagcgtg atggcaagtg tttactttgc aaaattaatg
    21901 ccaatttcac caatgatgaa atttgcactg tacttattag catttgtagt gggtgtttgg
    21961 atgtgcgtta gaacgcctaa tcaccctaaa gagcgaaata ttttaatgct gtattatctc
    22021 tttaaaatgg atagaaacag ataccacgaa gtagagttta aaagaaatag tagggggaaa
    22081 attgaattat gattgttgct gaaagtaatg aaaataaatt aagtggtgtt tttgacaaat
    22141 tcttagaact agttattcca aaagataaag atatgtgtga acttttacca tataaaaaaa
    22201 tattgaaaga cggaacgtta gtgactaatg acaataagta tcaagccttt tttaaagtta
    22261 aaacaactga cttaacttct atttcagaag atggatttat gcgtatggta aacaaactca
    22321 cttatttaaa tcgagtatat ttagaaccat ataaaattta ttcattaact tatcctactg
    22381 aaacagtaaa tcaagcgatt tattggaagc atagaataca ggaggcaaag agaagtttat
    22441 tgagtaataa tgatcccaat ttagaaacgt atctgaagtg gaatctaaag ctagcttacg
    22501 ataattatga aagagttgat ctagtatcga ctttaccaga gttgatgttc gttatttgtg
    22561 tttatggaaa aacacaaaaa gaagtgaaaa atcatgccga tgtaatgaaa caggccggtg
    22621 gtagagaatt agatttacaa agaattactg aaggggattt aatcaatgtg ataactaaat
    22681 tgatgaatcc caatacttct ttataaggcg gtgcttatta tttgttaaag cactgtagaa
    22741 aaggattttg taaagtaatt aaaggtaaaa atgctgctga tttaatcttt gaatggaaag
    22801 aatctaattt aattaatgta cgtgggaaaa atggaagcgg aaagacagaa agagtgatgg
    22861 ctagtattga taattcaagg cctagatcaa gagcattagt aataactgat gaaaaaaatc
    22921 actataaagg catacgaacg atagaaaaaa aatatttaac taacaatgat ttaaaagtag
    22981 tagatgatat tttaaatatt tatcctgatt ttaatttagt tgtttttgat gaaacaattc
    23041 ctgtgacaga aataaaagga ttctatcaaa gcttttccaa tctagcaaat aaatatgagg
    23101 aagttctgtt tgtttggatt acagaagaag atagtaatag caagtcagcg ttctttagtg
    23161 aaaagaacgc ttttttacat tctatttaaa aaggagaatt gaacatgatt gtaagtaaaa
    23221 aactagcaag aaatctagta agtgaagggt atgacttaag attagtagaa aagtatcaac
    23281 cgagcggtgg aattaaattt gatgagcgtt atatttctac tggtgatgga tatgtaactt
    23341 cgattgctgt tattgagttt gcagaagatg taaatagtcg atggttaacg gatataatgg
    23401 caattccaaa tactattgca actttcgatg tatgtacggc ggataaagaa gacttattac
    23461 acaaaatcaa tacgtctatt ggagaattag attaccgatc aacaaatgag aatagaacaa
    23521 ctgacaaaac agacgcttta ctagataaat acaatttaga agattttgca caagaaatta
    23581 cgaaacgtgg tgaagttgtc aaaactgttc atgctcgtat ttttgtttat agcgattctt
    23641 tagaagaatt agagaaaaga attagtgaga ttcgtaaaga tttggctagt gtcaatcaca
    23701 aggcaattgt tccgcaattt agaatgaagg atgaatggat gtctttattt caagatggca
    23761 tggaacaaga atcaaaatta tttgccaaga aaggcttaac tgttccggca aaaaatattg
    23821 gtggcggtat tccatttcac cacaagagtt tgaaagatcc acgtgggttt tatttaggaa
    23881 ctacgtcaac tggtggtgct tttatatttg atccatttta ttctacgaag caaagacggt
    23941 cgtttaacgg ctttatttta ggaaagatgg gttttggtaa gtcaacttta attaaacagc
    24001 tacaggaagg cctacaagcg aaaaattgtt ttattcgagg atttgacaag tcaggtgaat
    24061 ttgaaggcat ggtgaagaaa gcaggcggtg tatacgtcga tttaggcggt ggtgatatgg
    24121 tagtaaatcc attgcaaatt tatgcaacag tcatgaaaaa agatcaattt ggaaatgatg
    24181 ttgtagatga atcaaagagt tttcaaaatc atatcactaa gtgtagtaat caaattcgct
    24241 ttttagaacc tgaaattgat acggcaactg tacgtgaata tactttgatg ttacaagact
    24301 tttatattca ttatggaatt ttgccaaaag aatggagaga agcgagagac gacaacgaac
    24361 gaaatcaatt aaatacaaat atttgtaact tgcctaatga ttcatatcca atctatggag
    24421 agtttcttaa atttattgaa aatcaacaat tcaaaattaa aaatgctact cctgaacgtg
    24481 tccgttctat tgagctaatt aagcttatcg ttacggatat gtgtacaact tatccgcaaa
    24541 ttttcaacgg agtaacaaca atgccaaact ttgatacaac aaaaattgtt tactataaca
    24601 ttgattctat tagtaaaatg agtgccccag tattccattg ccaattattt acagcgttaa
    24661 cgactatttg gaacagtgcc ttgcaaaacg gaaagattca aaaagaaaac tttaaaaaat
    24721 atggcagtga ttattataag tatgcacaag atttcatggt tttgatcgat gagtgtca
//
LOCUS       DACEOM010000046        24390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEOM010000046 DACEOM010000000
VERSION     DACEOM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.047"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW781_001773"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW781_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1182772.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IW781_001774"
     CDS             complement(1551..2972)
                     /locus_tag="IW781_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1182773.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW781_001775"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW781_001775"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1182774.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IW781_001776"
     CDS             complement(5383..6639)
                     /locus_tag="IW781_001776"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1182775.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IW781_001777"
     CDS             7219..8520
                     /locus_tag="IW781_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1182776.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8589..9188)
                     /locus_tag="IW781_001778"
     CDS             complement(8589..9188)
                     /locus_tag="IW781_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1182777.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9442..10323
                     /locus_tag="IW781_001779"
     CDS             9442..10323
                     /locus_tag="IW781_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182778.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10336..11946
                     /locus_tag="IW781_001780"
     CDS             10336..11946
                     /locus_tag="IW781_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1182779.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12023..12682)
                     /locus_tag="IW781_001781"
     CDS             complement(12023..12682)
                     /locus_tag="IW781_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1182780.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12965..13630)
                     /locus_tag="IW781_001782"
     CDS             complement(12965..13630)
                     /locus_tag="IW781_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182781.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="IW781_001783"
     CDS             complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="IW781_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1182782.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14410..15393)
                     /gene="dhaK"
                     /locus_tag="IW781_001784"
     CDS             complement(14410..15393)
                     /gene="dhaK"
                     /locus_tag="IW781_001784"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1182783.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="IW781_001785"
     CDS             complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="IW781_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1182784.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15944..16264
                     /locus_tag="IW781_001786"
     CDS             15944..16264
                     /locus_tag="IW781_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1182785.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16376..17245)
                     /locus_tag="IW781_001787"
     CDS             complement(16376..17245)
                     /locus_tag="IW781_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1182786.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17341..18645)
                     /locus_tag="IW781_001788"
     CDS             complement(17341..18645)
                     /locus_tag="IW781_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1182787.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18642..19151)
                     /locus_tag="IW781_001789"
     CDS             complement(18642..19151)
                     /locus_tag="IW781_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182788.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19326..21461)
                     /locus_tag="IW781_001790"
     CDS             complement(19326..21461)
                     /locus_tag="IW781_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1182789.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21544..23199)
                     /locus_tag="IW781_001791"
     CDS             complement(21544..23199)
                     /locus_tag="IW781_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1182790.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23201..23515)
                     /locus_tag="IW781_001792"
     CDS             complement(23201..23515)
                     /locus_tag="IW781_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1182791.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23685..23945
                     /locus_tag="IW781_001793"
     CDS             23685..23945
                     /locus_tag="IW781_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1182792.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23959..24273
                     /locus_tag="IW781_001794"
     CDS             23959..24273
                     /locus_tag="IW781_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1182793.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6948 a         5042 c         4401 g         7999 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcata tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt
     8581 tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct
     8641 gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga
     8701 aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct
     8761 cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca
     8821 gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg
     8881 ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat
     8941 ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa
     9001 aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt
     9061 acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc
     9121 atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa
     9181 ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt
     9241 attctgaggt ttattataac tatctattga gaaagtatca agctagcaat tccttgttta
     9301 tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaagtt
     9361 ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt atatattttt
     9421 agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt gaaaaagagc
     9481 tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt
     9541 ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact tttagggatt
     9601 ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga agatagttta
     9661 tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg aaatttgact
     9721 ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa
     9781 gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa
     9841 ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga tctctattta
     9901 tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca agaagaagtt
     9961 gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct cagtgaagtc
    10021 gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga agaaggaaca
    10081 ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga agcagagaat
    10141 agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa
    10201 tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa attaggtgtg
    10261 aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca ttatgaagga
    10321 tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt ttctcaaaac
    10381 cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc tttttctggt
    10441 ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg attatacgaa
    10501 accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc gaatgctgct
    10561 gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct
    10621 gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga acttggtctg
    10681 attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct
    10741 gaaagtattc tgatcaattt gcttttagca atatcgatca gctgtacgat gatttttttc
    10801 caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt aggtagtatg
    10861 gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag ttcagccagt
    10921 tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc aataacagat
    10981 gtagctgatt cctccctttc gatgtggaat cctttaggct ggtcttattt gacctatcca
    11041 tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct aatcttgata
    11101 atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat ccccgaacgt
    11161 gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt attctatcta
    11221 aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc ggcatacggc
    11281 tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa acagatgttt
    11341 atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt agtcttgatt
    11401 tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga agaaacccac
    11461 ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta ttggaccaat
    11521 atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg tgcactaggg
    11581 ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat gtttcttaaa
    11641 gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg ctttctattc
    11701 ggatggcttc ctcgaatcgg taaacttgct tatatctact tagcttactc ctttgcactc
    11761 aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc tgtccctagc
    11821 tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac gttatcatgt
    11881 atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga cttactagaa
    11941 ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc aaaaatccaa
    12001 catttggagg tcattttttt atttacaagt cttctaactg ctctttatca atgatccaga
    12061 cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat tttctagata
    12121 aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt ggcaggaaaa
    12181 agtgatcctt ccctgtcgct ttagctaagt caagcagata gacagcgact cgttcttcta
    12241 ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc tcaacgttga
    12301 ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag aaatcttttt
    12361 gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac tcatttggaa
    12421 cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt aaaagctgtt
    12481 cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg atcactaatt
    12541 gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga tgtttgacaa
    12601 gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa
    12661 cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt ctaataaaga
    12721 aacataactt cccttattcc aactgataat tttcttttat catagcataa acgtcaagcg
    12781 aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt ttccttcttt
    12841 tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt gaataatcaa
    12901 aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc ttatttcgtg
    12961 ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt tcttgtaagg
    13021 tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat agtttaccca
    13081 tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt gtatcggaca
    13141 aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc catatgtaat
    13201 aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac tggttatacg
    13261 ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa tagatttggt
    13321 attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga gattcgcttc
    13381 ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt tcgattttag
    13441 gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga atctctttcc
    13501 atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct gttggatggc
    13561 tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa tctgattttc
    13621 caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa ttttgcgata
    13681 tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac agccaaacaa
    13741 aaaactgcga tgagcatcac cgcagtttta ttgaacttaa ggttgattga tcacatctaa
    13801 aagcgttgaa aataaaatgg cactagaagc agctcctggg tcgatatgtc cgattgcacg
    13861 ctctcctaga taggaagctc gtcctttttt agctaccaag actttggtgt gttcttttgc
    13921 ctggtcaatg atttcttttg ttaaattccc tgccttcaat gcctctgtga ctggcagcca
    13981 aacatctaac atcgttttat cgtcagcttc tgcctttcct cggctttgaa tacccgctgt
    14041 tccctgctcg atcacttcgc ctaatttttc ttgagaatca atagaatcga cctcttttgt
    14101 cgctttcatc atgttcatga atgcagatcc gtataacgga ccagaagccc caccgacttt
    14161 cgatatcatc gccatagaga atactttgaa tgtatcgctg attgtttcag gatgatcggc
    14221 ttgaaaagct tcttcataag ctgaaacccc tcttcccatg ttatttccat ggtctccatc
    14281 gccaatcggg gtatctagat cactgagata ttgtttgttc tgattgattg cctctgcaaa
    14341 ttgcgtgagc cattcttgga tttcttgaac ggttagtttc atattatcac ccttttatct
    14401 tgagattttt taccatgaaa tcgtctgtac attgctttct aaagaatctt tccattgtgt
    14461 ctccgtcaag tcgatcagtg ttagagaaag accttgcata tctagtgagg tcataaaatt
    14521 accgactttt cggaaaacta cattgacgcc tctttcttcc aacagagcta aaacatcatt
    14581 catgaacacg aactgttcca tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc
    14641 ttcttttggt aaattcgagt aatcatctaa aatcttagta acaagttcct ttgccagtac
    14701 tttagaaggc tgcatctttt ctcgacgata gcctggctca ccgtggatac caacaccata
    14761 ctcgatctca tcttctccta gttcgaatcc tggtttccct acttctggta cagttgctgc
    14821 tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc ttttctccca gttctttcaa
    14881 ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg attttgtgta caaggacagt
    14941 acctgcaact ccgcgttttc cagcagtata cgtactattt tcaacggcga tatcatcatc
    15001 tacgatcaca ctttctactt caatatcgtc catagcagct aattcctttg ccatatcgaa
    15061 gttcaatgca tcccctgtat aattcttaac gatcagcaaa actccttttc cactatcggc
    15121 ttccttaata gccgtctgaa tctgatctgg agttggagaa gtaaacacat caccaagcac
    15181 cgctgcactt aacatccctt cccctacaaa cccggcatgg gatggttcat gtccgcttcc
    15241 tccaccagaa accaaaccaa cttgttccgc tttctgattc ttggctacaa cacgggaaga
    15301 ttctacacga tgaacaagtt ctggatagct ttttactaat ccttccagca tttcttcgac
    15361 aatattgctt ggatcattca tgattttttt caatttattc ccatcctttc tttccggcat
    15421 tttgtgcttc tgttaagatt tgctgagtat catctgttat accggcagtg atggctgctg
    15481 caaaagctcc ttccactaaa ggcgcatcca ccagatgata acgttgctgc tgctcttctt
    15541 ccaacatgtc ttttgccagt tccgagttca ataccgcgct accgatatcc gcaaaaacaa
    15601 aataactagc tgcatcgctt gattcattga ctgcttctac gattttcatc gggtctgatc
    15661 cgatctcgcc atttattccg ccgagagaaa aaatttgcac ctcttcggat ttttgcatct
    15721 gctcaatcat ttctttaata ccatctgtta ctttttgact atgagaaata agcaaaatac
    15781 tttttttcac aagaatcttc ctttctttta catagttgct aacgattggt ttcgctttca
    15841 ttaatgaaca attcaagtat ataataaatt tctaattttg ggaaaatatg tgtagtgctt
    15901 cttgaagttt gttatacttt aattaaaaga gaggagtgat cgaatgatta caacaacaac
    15961 gaacagtgta gagggaaaag ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat
    16021 tactgggatc catgcattta aagacttgga ggcggggttg cgaaatatct ttggcggccg
    16081 ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa gaagcacttg ccgaaatggc
    16141 cgaacgtgca aaagcattag gagctgatgc gatcattggc gtaaaaatgg actatgaagt
    16201 attaggcagc gacaatggca tgctgatggt cacatgtagt ggaacagctg tctccacgag
    16261 ataagtaggg gacatctgtt ttaataacta aagagagatg ggacatgtag tggcactcaa
    16321 gaattagatt tttcagtcta acttcgaggt actctacctt cccatcctct cttttttatt
    16381 tctttttgcg gatgattccg taagctgctg cgactaacac acccgcccct gctaaggaat
    16441 aactgacaga agctgttttt tcatttgttt ttggcaaagt ctgacttgtt gatttggatg
    16501 gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga ggtcactgat ttttctcctg
    16561 ccgaatgcaa aatatctttt attcgttgga aactagctgc taagtcagct ttttttgctt
    16621 caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc gtggattgct tgactggccg
    16681 ctgtgatatc cgcttgagtt tgacttagct ttccagaagc tgtttgagca acctgattta
    16741 attgctgatt gaattctttt gcttgttgga tcttcccgtc aagatctgtt tggacagctt
    16801 ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg tgcacggtca tttcccactt
    16861 tatttttaag atcagctaaa tcttgatcga ttgctgcttg gtcttctttc aatttgtttt
    16921 ttgcagcttc taatgcttct tcgagttctt ttcttttatt ttccaagtct tgcaaatgat
    16981 cgatttggtc agaagaggta gaactattta ttgatgtatc ttctgaagat gaaatcgttt
    17041 ctcctgaatt atctgtcggt acattggaag tcatttctgt ttccgaggca ccgttttcag
    17101 caccagaaga gcttatggtt gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa
    17161 aagctgtctg tcccaataag aaaatactgg aaacagcaat tggccagatt aacggcttca
    17221 ttcttttttt catcactttt cccatcctta ctacttttta actaatttct tattataagc
    17281 aaaaatgttt ggaagaacca taaacacaac aggctgtttg tctaaaaaac ggtgcatctt
    17341 ttattttttt gtacgttccg ggcttttcca tgtcccgctt ccataaccta aaatcgtttt
    17401 gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc actatccaga acaaaagcat
    17461 atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt cgatcatcta caatcagcga
    17521 agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt
    17581 aaaagtaata taaatctgtt cgtagtttcc ttgatataca taatgcccga ttaatccagc
    17641 aaaaagaact acggaaatac aaaagaatat tgaccagaca atactcaatg tttgatcaat
    17701 gaagatgatc gttcttccaa tatgtttaaa aggatggagc atcacttttt tgaaattcgt
    17761 cagccagact tctgttccac cttttgccca acgttttctg tgtcgatata gcatcttcaa
    17821 cgtcacaggt acttccatga aaaagataat ctcaggagca aaaacagata accagtcatt
    17881 taactgatga tcccaagcaa tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc
    17941 cacatcgatc aaggcttctt ttcgatacat cgtatttgct ccactgtacg cataaagacc
    18001 accgaaaaca gctgactgtg ttcgtttaat gatgcccacg atacttgaga attcaaccgt
    18061 ttgagatttt gcaatcaatt tggtacgatt ttggacatcc atatttgcag tgaccgcagc
    18121 aatatgtctt gcaccagggc gaatgaaata attcacatac ctaatcagtg catctggctc
    18181 cggtaccgtg tctgcatcat tgcttaaaat caatttccct tttgcaaagg caagtccgat
    18241 attaaacgcg tgggcttttc ctttattttt ttcgatccga accacacgaa gattagcgta
    18301 ttttttcatc agtctagcta atatttctgg tgtttggtcc gttgaaccat cgtctgtcac
    18361 taatacctca tagttgtgat aattcaattt cgtcatcaaa tactcgattg tatattcgat
    18421 cacaatttct tcattatggg ctggcaccat gatcgtgatg aacggttcga cactcaaggg
    18481 aacatctacc cattcttttt gcttatgttt gtataaaaac acataacacc agacaccaat
    18541 aaaccataca aatccgccta aaatcggata agaaacgaga aaaaaataca taatagacaa
    18601 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
    18661 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
    18721 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
    18781 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
    18841 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
    18901 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
    18961 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
    19021 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
    19081 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
    19141 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
    19201 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
    19261 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
    19321 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
    19381 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
    19441 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
    19501 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
    19561 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
    19621 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
    19681 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
    19741 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
    19801 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
    19861 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
    19921 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
    19981 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
    20041 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
    20101 atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa
    20161 gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta
    20221 tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga
    20281 tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta
    20341 ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt
    20401 ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt
    20461 tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct
    20521 cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa
    20581 accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc
    20641 gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc
    20701 gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca
    20761 cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt
    20821 tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag
    20881 acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga
    20941 tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga
    21001 accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc
    21061 caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag
    21121 gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc
    21181 aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga
    21241 tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg
    21301 tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt
    21361 gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc
    21421 ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt
    21481 taaagtggtg gaaccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga
    21541 gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc
    21601 accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct
    21661 acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg
    21721 gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc
    21781 ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg
    21841 ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt
    21901 tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc
    21961 gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc
    22021 ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt
    22081 cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat
    22141 gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc
    22201 agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat
    22261 tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat
    22321 aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat
    22381 atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc
    22441 gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa
    22501 atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc
    22561 agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa
    22621 agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac
    22681 ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac
    22741 gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac
    22801 atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg
    22861 ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg
    22921 tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac
    22981 atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc
    23041 actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc
    23101 tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga
    23161 cattccacct gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc
    23221 gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta
    23281 attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt
    23341 ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat
    23401 gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct
    23461 tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct
    23521 gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga gctttattaa
    23581 tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc
    23641 gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag attgataata
    23701 aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga
    23761 tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta
    23821 ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg
    23881 atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa
    23941 agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca
    24001 gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta
    24061 tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat
    24121 ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa
    24181 agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg
    24241 caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc
    24301 agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa
    24361 aggttgtgaa aacgctgtcc tgcatcaagt
//
LOCUS       DACEOM010000047        23829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEOM010000047 DACEOM010000000
VERSION     DACEOM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.048"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..787)
                     /locus_tag="IW781_001795"
     CDS             complement(53..787)
                     /locus_tag="IW781_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1182794.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IW781_001796"
     CDS             1114..1524
                     /locus_tag="IW781_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1182795.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IW781_001797"
     CDS             1517..2218
                     /locus_tag="IW781_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1182796.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IW781_001798"
     CDS             2218..3804
                     /locus_tag="IW781_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1182797.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IW781_001799"
     CDS             3823..4044
                     /locus_tag="IW781_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1182798.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IW781_001800"
     CDS             4041..4802
                     /locus_tag="IW781_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1182799.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IW781_001801"
     CDS             5182..5691
                     /locus_tag="IW781_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1182800.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IW781_001802"
     CDS             complement(5766..6305)
                     /locus_tag="IW781_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1182801.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IW781_001803"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IW781_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1182802.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IW781_001804"
     CDS             7372..8169
                     /locus_tag="IW781_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1182803.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IW781_001805"
     CDS             8197..8832
                     /locus_tag="IW781_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1182804.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IW781_001806"
     CDS             8852..9625
                     /locus_tag="IW781_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1182805.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IW781_001807"
     CDS             10033..10830
                     /locus_tag="IW781_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1182806.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IW781_001808"
     CDS             10808..11221
                     /locus_tag="IW781_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182807.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IW781_001809"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IW781_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1182808.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IW781_001810"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IW781_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1182809.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IW781_001811"
     CDS             15998..16558
                     /locus_tag="IW781_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1182810.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IW781_001812"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IW781_001812"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1182811.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IW781_001813"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IW781_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1182812.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IW781_001814"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IW781_001814"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1182813.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IW781_001815"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IW781_001815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1182814.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IW781_001816"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IW781_001816"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1182815.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IW781_001817"
     CDS             21905..22483
                     /locus_tag="IW781_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1182816.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IW781_001818"
     CDS             22535..23017
                     /locus_tag="IW781_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1182817.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IW781_001819"
     CDS             23134..23508
                     /locus_tag="IW781_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182818.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5116 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtttt
//
LOCUS       DACEOM010000048        23674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEOM010000048 DACEOM010000000
VERSION     DACEOM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.049"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            289..696
                     /locus_tag="IW781_001820"
     CDS             289..696
                     /locus_tag="IW781_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182819.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="IW781_001821"
     CDS             710..1111
                     /locus_tag="IW781_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182820.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="IW781_001822"
     CDS             1113..1484
                     /locus_tag="IW781_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182821.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1520..1822)
                     /locus_tag="IW781_001823"
     CDS             complement(1520..1822)
                     /locus_tag="IW781_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182822.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2071..2271)
                     /locus_tag="IW781_001824"
     CDS             complement(2071..2271)
                     /locus_tag="IW781_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1182823.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2576..3808)
                     /locus_tag="IW781_001825"
     CDS             complement(2576..3808)
                     /locus_tag="IW781_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1182824.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4062..4631
                     /locus_tag="IW781_001826"
     CDS             4062..4631
                     /locus_tag="IW781_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1182825.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4809..5249)
                     /locus_tag="IW781_001827"
     CDS             complement(4809..5249)
                     /locus_tag="IW781_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1182826.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5407..6171
                     /gene="map"
                     /locus_tag="IW781_001828"
     CDS             5407..6171
                     /gene="map"
                     /locus_tag="IW781_001828"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1182827.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6203..7126
                     /locus_tag="IW781_001829"
     CDS             6203..7126
                     /locus_tag="IW781_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1182828.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7202..8341
                     /locus_tag="IW781_001830"
     CDS             7202..8341
                     /locus_tag="IW781_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1182829.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8334..9134
                     /locus_tag="IW781_001831"
     CDS             8334..9134
                     /locus_tag="IW781_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1182830.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9134..9961
                     /locus_tag="IW781_001832"
     CDS             9134..9961
                     /locus_tag="IW781_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1182831.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9939..10673
                     /locus_tag="IW781_001833"
     CDS             9939..10673
                     /locus_tag="IW781_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1182832.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10773..11639
                     /gene="rfbA"
                     /locus_tag="IW781_001834"
     CDS             10773..11639
                     /gene="rfbA"
                     /locus_tag="IW781_001834"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1182833.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11653..12225
                     /gene="rfbC"
                     /locus_tag="IW781_001835"
     CDS             11653..12225
                     /gene="rfbC"
                     /locus_tag="IW781_001835"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1182834.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12247..13275
                     /gene="rfbB"
                     /locus_tag="IW781_001836"
     CDS             12247..13275
                     /gene="rfbB"
                     /locus_tag="IW781_001836"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1182835.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13373..14224
                     /gene="rfbD"
                     /locus_tag="IW781_001837"
     CDS             13373..14224
                     /gene="rfbD"
                     /locus_tag="IW781_001837"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1182836.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14259..16292
                     /locus_tag="IW781_001838"
     CDS             14259..16292
                     /locus_tag="IW781_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182837.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16336..17616
                     /locus_tag="IW781_001839"
     CDS             16336..17616
                     /locus_tag="IW781_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1182838.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17826..18632
                     /locus_tag="IW781_001840"
     CDS             17826..18632
                     /locus_tag="IW781_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182839.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18644..19864
                     /locus_tag="IW781_001841"
     CDS             18644..19864
                     /locus_tag="IW781_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182840.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19854..21440
                     /locus_tag="IW781_001842"
     CDS             19854..21440
                     /locus_tag="IW781_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1182841.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21479..23617
                     /locus_tag="IW781_001843"
     CDS             21479..23617
                     /locus_tag="IW781_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1182842.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         7568 a         3939 c         4853 g         7314 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta tttttttatt taatctttgt atctttctgg atcaacgaaa gtatacttta
     1561 tatagtcata acgccgatga tcgctacgtg cgtccggcac gtcagtcacg atatcaaaca
     1621 aaaagtatac acccttcttc attctagttt tcgcagcagg aattttgaaa tagttcttat
     1681 tagaatagta gagattgatt aataagctat cttcgattgc taaaaagaaa acttctgaat
     1741 tccatacttt ataaaaatct ttgataaatc tattcgaagg gtcaaattta aaccataatt
     1801 gtgtttttcc ttccatcagc ataaaagttc acctcaaaaa gagtatacga acgaatgttc
     1861 tttttgtgaa ggcggaattt aatatacgga ataatagaaa acaagtactt gtgccaaatt
     1921 atgtgccaaa aaaattcgaa ttcaacaaaa tgtaaacaaa tagaaaacct tctatattaa
     1981 gatttcctgc aataacaaac acaacagaag acgtgtaata gttagtcagg aacgtacaaa
     2041 taacccctgt atcctttgcg gtacaggggg ttattttgta taagtagaca ttgtagtttc
     2101 taaactgcta tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa
     2161 aatagatgca tgcgtatgtc ttagtccatc aataaacagt cgaagagaaa atagggaaaa
     2221 attgattact aaagaaaaac ttactaggtc gacggtgtag ttgattttca tacttcaata
     2281 gattcgaatt aaacgatttc gattggctta taccagtcct atccaacgaa agatatatcg
     2341 ttcttaactc tagcggtata acttgaagct ttacgtaaag ccaatgggaa tgaactggag
     2401 atgacttttt attattttta gtaatagctg atgggttaat acttacaact ttccttttca
     2461 aagtaccata taaaaaaacg gtggggttgt ttagcgaaat aaaggatcta agccaatagc
     2521 ttagatcctt tattctatgc tttatattat tttttgcatg tgtgcgaaag tatactcaag
     2581 cccaatcgcc atttcggaaa atcggtactg tagagccatc ttcacggatt ccgtcgatat
     2641 ccatcttgtc agatcctacc atgaaatcta cgtgggtttg acttctattc aatccagctt
     2701 ctgctaattc ttcatcggac atttctgtac cgcctttcac actgaaggca taagcagaac
     2761 ctagtgctaa gtgattcgat gcgttttcat caaataatgt attgaaaaag atgatgccag
     2821 attgagaaat aggtgaaggg tcaggtacaa gtgcgacttc tcctaaacga cgcgcacctt
     2881 catctgtatc aagcagtttt gctaagacat cttctccttg ttcggcagaa aaatcaacaa
     2941 cttttccatc tttaaaagta aatttcatac ccgagatgat ggtgccagca taactcagtg
     3001 gctttgtact tgaaatgtaa ccatctactc gatgactgtc aggtgctgtg aacacttctt
     3061 ccgtcggcat atttgccatg aatttttctc cacgagcatt atagcttcca gcgccttccc
     3121 aaagatggtt tttaggcaat ccgataatga tatctgtacc gggagcagta tagtgaaggg
     3181 cagaaaattg ttctcgattc aattcttctg ctttttttgc taatttttca tcatgcttct
     3241 tccatgctaa gacagggtct tcttcataga cacgagtcgt tttgaaaatc tgatcccata
     3301 atgcctcaac ttgtttttct tctggcagtt ctgggaagac ttttgctgcc cattgtttac
     3361 cagccgcagc cacgacagtc caactgactt tatttgcttg agttgccttg cgtaaattca
     3421 ttagtgcttt gccattcgct gattgataag acgctactcg atggctatct acaccagcaa
     3481 atgcatcggg atcggcagaa acaacgctga tcctgcttgc tcctttttca agccattcat
     3541 ccgcttgatc gattttagat tgtggtacat tctcaatacg atccgttgca gcatgtaaaa
     3601 gaaattctct ttggatctgg tcgtcagtcc attgaacgat aacctcagcg gctcctagtt
     3661 tataagcttc ttgtgtgatc aatcgagcaa ggggtgcctg ttccacgctg atctgtaaaa
     3721 caactgtgtg tcctttttct gtggctacgc cagtttctgc aattaagcgt gcatattttt
     3781 ttagaagttc attaaaatca gataacataa taaaaattcc tcctcaccat tttttctggt
     3841 aactattagc atgataccat ttaattatga ttcaaacaat gaggctttaa ataaaaaaag
     3901 gaggttattt ttttaaggtt aagtttcatg atatatgtta ctacttgcta tttcagcttg
     3961 tgaagaaaat caaaaactaa ttttgcatat caaaaaaaca tgttacaatg aaattaatca
     4021 atggtggaag cgttataaaa aaattaagga gattaaaaaa tatggcaaga aaaaagacga
     4081 ttactagaga acagattttg gcagctgctt atgaagtagc tgccaccgat ggatttacac
     4141 gatttactgc gagaaacatc gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg
     4201 agtttaaaaa tatggatgac ttgagacaag ctctttttga gcagatctat gatcatttac
     4261 aatatgaagt gttcactgtt gagcatacag ggaatacgat tatcgatctt gcttttaatt
     4321 atatccattt tgcaagaaga gagaagaagt tgtatcgttc actgtactta gaagaatatg
     4381 gtggaggaaa agagatgcag aatttctctt atacttactt tgtgaataca gtgaaacgcg
     4441 atccagctta tgtgaatctt tcagaagagc agatcgaatc tttgtataat gggacatgga
     4501 tcatagctac tgggatcgca gctttgatgt cttcaagcat catccatcca actgatgaac
     4561 agattgaaaa gatgatccaa gacagtatag atgctatctt gaaattcaaa gaaccaatcg
     4621 aaatcgattg aatatactca aaagttccac aaatcagtag gtaacaagag atgtgtcaaa
     4681 ataaggaaaa gttcgttttt cttacagcgt atgcattttt aactcccgaa gaactcttcc
     4741 atgaatgcaa tgaataaaag agccatacag attgaataaa tctggatggc tcttttattg
     4801 caagtatatt aaatttttgc gactagttct ttagcgaaag cttctaaatt ttcgatatct
     4861 tcttcttcag cattcagatc cactttaaca ttttcggctc cttttgatgc gcctgttttt
     4921 gcaaaagcag catcgaaatc atccaccgat ttgcagaatt catcgtaaaa agtatcacca
     4981 gatccgcaga caccgtaaat ttttcccaat agatccaatt cttgcaagtc ttcataaaag
     5041 tctacgatct catcaggcag atcgccatca tcgtatgtat aagtagctac tacgcaaata
     5101 tctgcctcct cgaattcatc tgcatctact tgggtacatt cgttgatttc gacttcgata
     5161 tccagatttt caaatgcttc tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg
     5221 ctagcatata cgatttttgc taaagccatg atgtgcctcc tcgtttataa ttatttaaca
     5281 attatgaatt ataccaaaac atcattgaaa agaaaataaa acctaaagac acctggggaa
     5341 tgtcatttgg ctaaatgtat gccgctatgt taaaatgaga gggatttgta aaaaggagac
     5401 gaaaatatga ttactttaaa atcagcacgt gaaattgaaa tgatggcaga atctggagct
     5461 ttattagcag atgtacataa aaatctgcgt gattttataa aaccaggaat cacaagttgg
     5521 gatattgagg tatttgtcag aaactatatc gagagtcatg gcggaattgc tgcgcaaatt
     5581 ggctttgaag ggtatgaata tgcgacttgt atcagtataa atgatgaaat ttgtcatgga
     5641 ttccctcgta aaaagccttt gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat
     5701 ttgaaaggcg gcatttctga ttcttgttgg gcctatgtag ttggagaatc tacacctgag
     5761 atcgaccgat tgatggaagt aacgaagaaa gctttgtaca tcggtattga gcaagcacaa
     5821 gtagggaatc ggattggtga catcggtcat gctatccaaa cttatgtaga aggcgaaaat
     5881 ctagcgatcg ttcgtgattt tattggtcat ggcgttggtc ctacgattca tgaagaacca
     5941 gcagttcctc attacggaga agctggaaaa gggttgcggt tgaaagaagg catggttatc
     6001 acgatcgaac ctatggttaa taccggaact tggaaaatga aaatggatcc taacggctgg
     6061 actgcgtata caagagacgg cggactttct tgccaatatg aacatacatt ggctattact
     6121 aaagatggtc caagaatttt gacatcacaa ggagaagaag gcacttattg atctatccag
     6181 tcaataggta cgggagaggt acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct
     6241 gatgcgcttt attgagacga ctcaaaagcg catcactgat tcagaaatga gtacgacctc
     6301 tgtcgtcgtt gcttattatt tactcttgtc tttatttcca ttgattattg cttttggtaa
     6361 tattttaccg tttctccaca tcgatcaaga gactgtactg acctatttac gagaagtgat
     6421 tcctgagacc gtttaccagt ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc
     6481 aggaggcttg ttatccgttt ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa
     6541 tgctttgcaa attgcgatga ataaagcgta tggcgtagaa aatagaaaaa attttttgat
     6601 cgtccgtttc ttctcactaa ttgtcattgt cttgttcatg attgctatta gtggggtaac
     6661 tatcgttctt gggttgggac agctgatttt ggatgctatt cagccaatcg tacaaatacc
     6721 cgtgaacttt atcgatcagt tccaaacttt gaagtggcca atcacattgg tagctttatt
     6781 tgtcatcatg ttcttgattt atttaattgt ccctaatgct cagttgaagt tgaagacgat
     6841 cattccgggc acgatcttcg ctactgtcgg ctggatgctg ttatcacaag tgtttagtat
     6901 ctatgctagg tattttgcaa cgaggatcag cggttatcag atcataggaa gttttattgt
     6961 actcatgctt tggcttaatt ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc
     7021 agttgttcaa gaatacgtga cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa
     7081 atggttcaat aaattgaaaa ctaaattttc tcataaaaaa aagtagaaac aagagttctt
     7141 attgtaaatt tatatcagta atttataata attatgactc ttaattaaag taggtgccca
     7201 tatgcttgtt tcttttagta tggttataag attttttctg acaattttat tatcactgat
     7261 attaacacct gtattcaaaa tcatttcagt acaaacaggt atggtcgaca aaccaaatga
     7321 aagaagaata aataaagttc caatgccttc agcgggcgga ctgcctatct ttatttcatt
     7381 tgttatatcg actttattct tattccgtaa aattatccct caatcgtata ttgtaccgat
     7441 cttgattgct tctttagtaa tcatagtcac aggagtgctc gatgataaat atgagctgac
     7501 acctaaacaa aaaagtatag ggatcttgat tgcttctctt attatttatt ttgtagcaga
     7561 tatacgtatt gattccttca cgcttccttt tattggatat atccaactag gctggcttag
     7621 ttttcccgta acgatttttt ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg
     7681 tttggatggg ctggcggcag ggatttcatt gatcggttta attacgatag gaatcattgg
     7741 atattttttc ctccatgctt ctacagtgta tattccagtt gttattttct gtcttgtagc
     7801 aagtattatc ggtttttttc cttacaattt ttatccggca aagatttatt taggcgatac
     7861 aggagctcta ttcctagggt ttatgatggc cgtcctttca ctacaagggc tgaagaacgt
     7921 gacttttgtt tcatcgattt ctttgctgat cgtcatggga gtgccggtaa cagacacgtt
     7981 ttttgctatc attaggagaa aagcaaaccg tgtttctttt tctacagcag ataaaaaaca
     8041 tcttcatcac cgactaatca gtttaggctt tactcataaa ggtgctgtcc tgacgattta
     8101 tgcgatggct ttgatgtttt ctttcacagc tatggtcatg aattatactg gaagaatcgg
     8161 aacgatcata ttgattattg ccatgttgtt tgctgcaatt ttattatttg aattgattgg
     8221 tctgatcaat gagaaacatc agtttatttt aaaaacattg cgatttttag ggaataaaga
     8281 gtaccgtacg caagtaaaac agaaatattt gaagaaatgg aaccgtcata tagatgaata
     8341 acaaattaaa ccagccgatc cttattagta ttgtgacggc aaacagtgat aaaatttttc
     8401 ggacactaga taattttatt tcaactgtaa aagatgaaac agcagtaaaa atccgaattt
     8461 ttgacaacaa ttcacttccc gagtacaaaa aaagactaac agagtatagg aaatatccct
     8521 acatcgatat ctattttcat gatgaaaatt ccggatttgg ttatggacat aatcacaatc
     8581 tgatagctag cgattctcgc tatggcatta tttgtaaccc agatatttta gtggatgaag
     8641 agacagttac agcattggtc ttgttattga aagaacatcc ggaatgtgcg atgttagcac
     8701 ctaagattct taatgaagat ggaacaactc agcacctcat ccgtgagcag ctggctgttt
     8761 ttgattacgt tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac
     8821 tggctgtgtt tgaatgccga ggattgccta ctgatgctaa tagttatgta cggatgatct
     8881 caggtagttt tatggtagct gatattgata aatttaaaga gataaagggg tttgatgagc
     8941 gtttcttcat gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca
     9001 agttattgta cacacccttt tatgcagtta tccattttta cgggaagggt gcgcatcgaa
     9061 atgcaaaact gtttcgcgtg tttttgactt ctatgatgaa atttttcaat aagtgggggt
     9121 ggcgtttttt ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc agcaactatt
     9181 cagtcaatct ccagcttatg gaccattaag caaagcactt agcgaaaagc aaattcagct
     9241 tattatttac gataatagtc ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat
     9301 cgtttatcat catgatccgc aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc
     9361 tcaagcagat gaaggaacac gttgtctcgt cactctggat caggatacga ggctagtcga
     9421 cgattatttt gaactattgc gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc
     9481 gatgattttt tctgggagcc gacaagtttc tcccctatat tcagatcgtt atatcaatcg
     9541 tacagctcaa gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg caatcaattc
     9601 aggtgttgcc tggtctgtca aatttctaaa aaagattggc gggtttaatc ctgctttttc
     9661 tttagatttt ttggatcatt ggctgttttg gaaagtggat cagttggaaa aaactgtgga
     9721 gatccttcct gtacgaatgg aacatgatct ttcggtttta gactatgaaa aagtttcaag
     9781 taatcgatac cgttctattt tacaagcaga aaatcgattt tatcatgaat atgatcaaag
     9841 gcatttatcc agtcaccgac gacaattatt gttaagaaca gctaaacagt tcttgaccgt
     9901 caaaaaccgg catatctggc ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg
     9961 aattcgtgcg ttcggtttgt atagctacct ttaatggagg cagatattta agagagcagt
    10021 tagatagtat tttatctcag ctttcagcag aagatgaagt agtcatttct gatgatgggt
    10081 cagtagatga tacatgggat atattaactg aatacgcaga aaaagataag cgaatccagc
    10141 ttttttcggg gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc cagcagacga
    10201 aaggagatct aatctttttg gcagaccaag atgatgtttg gttggagaca aaagtgaaaa
    10261 agatcactga ctattttgag cagaatccgg atcagctagt agtagtttct gatttaatca
    10321 ttgtggacag cgagctgcaa gagatccatg attcgtattt ttcgtttcga aattccaaag
    10381 aaggatggct taataatgtg ataagaagtc actatattgg tgctggtatg gctttccggt
    10441 cttctttaaa acccgatatt ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga
    10501 ttggtttgct gggaggaaag aagactgggt tcctaagaga accgttaact ctgtaccgaa
    10561 gacatgattt aaacgcgagt gagattgaaa cacattctaa attacggcaa aaaatttctt
    10621 ggcgtgttca tctgattacg gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga
    10681 tgtttaaatc cttaatattt ccatgacaaa tataacaatt gatgagataa taacaaagtg
    10741 aaatttgaaa tcaaaaagga gagcagatat ccatgaaggg aatcattctt gctgggggca
    10801 gcggaacacg tctgtaccct ttaacaaagg caacatcgaa acaattgatg ccgatttatg
    10861 acaaaccaat gatttattat ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc
    10921 tgattatttc aacgccacaa gatacaccac gtttcaagga attatttgga aacggaaatg
    10981 atttaggact gcagatcgaa tatgcagtac aagaaagtcc agatggattg gcacaagcgt
    11041 ttatcattgg agaagaattt attggagatg atagtgtctg cttggtatta ggggacaata
    11101 tttactatgg tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg
    11161 cgacagtatt tggctatcat gtcaatgatc cagaacgctt tggtgtagta gaatttgatg
    11221 agaatatgca agctttgtcg attgaagaaa agcctgctca gccaaaatca aattacgctg
    11281 taactggcct ttatttttat gacaatgagg taatttctat cgctaaaggt atcaaacctt
    11341 cagaacgtgg agaactagag attacggatg tcaataaagc atatttggat aaaggaaaac
    11401 tatccgtaga acttatggga cgcggatttg cttggcttga tacaggaaca catgaatcat
    11461 tgcttgaagc atctaccttt atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt
    11521 gtttggaaga aattgcttac cgtatgggct acatcgataa ggaacaattg ctggcccttg
    11581 cacagccatt gaaaaaaaat caatacggtc aatatttatt gaatttggcc gctgaataag
    11641 gagtccttac agatgaaagt aacagataca aaattaacag atgtcaaaat cgtagaaatg
    11701 gacgtctttg gcgatcatcg cggtttcttc acagaaagtt attcaaaagc aaaatttgca
    11761 gaacatggat tgaaatatga ctttgttcaa gacaaccatt cactatcagc tgaagctggt
    11821 gtccttcggg ggcttcattt ccaaaaagga gaagctgcac agacaaaatt gattcgggta
    11881 gtcactggag cagttttaga tgtagtagtg gatatccgca aaggtagccc gacttatggt
    11941 caatgggacg gatttatctt atctgaacat aatcatcgac agttgcttgt acctaaagga
    12001 tatgcccatg gattcgttac tttgacacca aatgtgaact tcctttataa gtgtgataat
    12061 tactataatg cagaagcaga tgggggcatc gcatttgatg atccagattt agcgattgat
    12121 tggccaattg atccatcaag agcgattatg tcggaaaaag atcaccgtca tccgacatta
    12181 aaagaatttg aagcagaaaa tccatttgtt tacggagaaa tctaagaatt aaggagaaat
    12241 tttcttatga aaaacatcat tgttaccgga ggtgccggat tcatcggctc taatttcgtt
    12301 cattacgtgg taaacaatca tccagaagtg catgtcactg ttttggataa actgacttat
    12361 gcaggaaaca aagagaactt ggccggactt cctagcgatc gcgtagaatt ggtcgtagga
    12421 gatgttgcag atgcagaatt agtggaccgt ctagtaaaag aagcggacgc agttgttcat
    12481 tacgcggcag aatcccataa cgataattca ttaaaagatc ctttcccatt tgtacaaacg
    12541 aatatcattg gtacgtatac acttttagaa gcttgccgca agtatgatgt acgctatcat
    12601 cacgtatcta ctgacgaggt ttacggcgat cttccattac gtgaagacct gccaggacat
    12661 ggggaaggcg aaggagaaaa attcactgcg gaaacaccat ataatccttc aagtccatat
    12721 tcttctacaa aagctggttc agatctgtta gtaaaagcat gggtgcgttc ttttggctta
    12781 cgtgcaacca tttcaaactg ttcaaacaac tatggtcctt atcagcatat tgaaaaattt
    12841 attcctcgcc aagtaacgag tatcttaagc ggtattcgtc caaaattata tggcgaagga
    12901 aagaatgttc gtgactggat ccatacgaat gatcattctt cagcagtgtg gctgattttg
    12961 acgaaaggca gaattggcga aacctatctg atcggtgcag acggtgaaga agataataag
    13021 accgtgatgg aattgatctt agaaatgatg ggccaaccaa aagacgcata cgaccatgta
    13081 aacgaccgcg cagggcacga tttgcgttat gcaatcgatt cgacaaaatt gcgagaagag
    13141 ctaggttggc agcctgagtt tacgaacttc cgtgatgggt tggctgacac gatcaaatgg
    13201 tacgaagaac atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc
    13261 aaaaatggac aataatacag aggaaatttc actgtattaa tggaaaaaca catgtgaggc
    13321 aggcgctcgc ctgtctcttt tttaaaataa aaatcaagga gcggataacc atatgttttt
    13381 attaactgga ggaaatggac aattaggtac agaattgcgc catttattag atgaaaaaaa
    13441 tctgccatat gtttcaacag atgcaaaaga cttggatatc acagatgctg agaaaacaat
    13501 ggcttatatt acagaattaa aacccgatgt tattttccat tgcgcagctt acacagcagt
    13561 agataaagca gaagaagaag caaaagaatt agatgagaaa atcaatgtcg atggcacaag
    13621 aaatgtagca cttgcagcaa aagctgtgaa tgcacaattc gtctatatca gtacagacta
    13681 tgttttcgat ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa cgaatccact
    13741 aaatgaatat ggtcgtacga aattattagg cgaaaaagca gtagaagaga ttttagaaga
    13801 ctattatatc atacgaactt cttgggtttt cgggatctat ggacacaatt tcgtcttcac
    13861 gatgcaacgc ctagcggaaa cccgcgacaa actgactgta gtaaatgacc agtttgggcg
    13921 accaacttgg acacgtactt tggcagaatt tatggtatat gtcatcgaag aaaaagcacc
    13981 atttggtatt tatcatttgt cgaacgaaaa tagctgttct tggtatgagt tcgcaaaaga
    14041 aatattgaaa gatacagatg tagaagttgc acctgttacg tcagaagaat ttccgcaaaa
    14101 agcaacacgt ccgcagtatt cagtcatgag cctgaaaaag acagaagcgt taggttttgt
    14161 cataccaact tggcaagaag cacttgcgca aatgctggaa gctgccaaaa aaatgaataa
    14221 gtaatttcct ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg
    14281 cacgacgaaa agaaaagcca aaagcgaatc aggcagattc gaatttggtg cttgacctgt
    14341 ttctaaaact ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt
    14401 taaacggaag ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa
    14461 aaactgatgt gatttttgga cagaatcggg aagtgcggtc agacgaatgg ctggtgcaga
    14521 caccttttta cctctcacaa gctgaagagg acttttcgtt ggtgaacgag gattattcat
    14581 taaacggaca aaacatgatc attgcgtata attctcctgt aaaggatatt acggttatcg
    14641 gaaaaccatt caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt
    14701 gggcgatgaa attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga
    14761 agaggaaaaa agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc
    14821 agtggtggtt catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgattg
    14881 ctttttatca ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt
    14941 tggtggttat tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc
    15001 tggcgtactt gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt
    15061 catgggatct gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca
    15121 tggttcattt ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc
    15181 caggaaaacg ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta
    15241 caaattggaa aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt
    15301 tctatcactt ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag
    15361 atagcgaaca aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt
    15421 ggattaccgt tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc
    15481 cattaagagc catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg
    15541 cttatatttg gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa
    15601 atgcatggtc ttatttctat gcgattacgc attcgaaaat ggataattat ttttcggatt
    15661 ttgaaaccat cgcagttgct gtgttgggaa cagtgatttt agcttttgca ttatttggct
    15721 ggagacgctt gttttacctc gcttttgtcg ccttagttat cgtatctggc tttttcgtca
    15781 atccagtcgt agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa
    15841 aaatcgatca acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca
    15901 ccccaacatt gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat
    15961 ggaagaaaat cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa
    16021 gagcttttat cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg
    16081 tcacgttgaa ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac
    16141 cattaactga ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata
    16201 aagacggtta tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac
    16261 aaactcagga aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata
    16321 ttagtagaaa ggagagtgaa gataattcaa gcaatcgtac aaaaatttgt caacaaaatg
    16381 agtgtcggtt tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcaggggta
    16441 ggttttccag caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg
    16501 gctattttgc tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt
    16561 gttgcgcttg cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta
    16621 tttggagact tactgtttcc tttgattatt ttattttgtt tagtaattaa atggatcgag
    16681 tggacaaaat gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg
    16741 ttatatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc
    16801 aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt
    16861 gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgttcg aatcattggt
    16921 gggatagcag tcgttgcagc ctttttaggt atttgggtct gccaatctag aggtgcatta
    16981 gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt
    17041 cgtgcgccat ttattgccta cacggttacg attcttttag ctttgccgat ttcttatctg
    17101 gcggctgtct ctgaaaaagt caatttgttt accggtcgag aagatatttg gcataaattc
    17161 tatcagacgc taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc
    17221 cagcgaggaa accaattttt aggaaaccac aatagttata attctatttt gaatatctat
    17281 ggattgatag gatttgggat tgctgctttg ctgcttatcc tttttattgg tcgcttaacg
    17341 ttgaaagcag atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg
    17401 atgcagtcat ttatggagga tacattgact tcatacccat gggttccgat tatttatatc
    17461 ctgttggcta tggccgttca tcgatatgat gaaccagaag ttacggagga attctatgtt
    17521 atggaagagg catatgacga tatgacttat gatgatacat cttatgagga acaaccagat
    17581 agcgctcatt cttccaggat ccaacgctat cattgatagg cacaaaatac gagagaggct
    17641 gtgacaaccg tcgcagcctt ttttggttta ttgcaattgt ctagcatctt ctgtaaaata
    17701 gaaacgttag agaaaatctt gtatcataga aaattaagaa agaaataaga acgcagctcg
    17761 gtaccaaaaa tatggtaaaa tggcacttga ctgtaattca ctgaaaatcg cttggaggaa
    17821 cacaaatgtt taaagatttg tatttattta tgaaggacat ttatcagaac agaaaattac
    17881 tgctacagtt ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct
    17941 tatgggcgtt tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg
    18001 gactgaaagc acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga
    18061 tgattccctg gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat
    18121 atacttattt agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc
    18181 tatccaatct gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa
    18241 atggtatata tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg
    18301 ctttcttgac aggattgact tggctgacgg catcgatcca accattttta cctgatatca
    18361 tgcaattaat tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc
    18421 ctaatcagtt tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg
    18481 ttcaaggtta tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata
    18541 ctttgtattt ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta
    18601 gattgaaacc acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat
    18661 tagactaaaa gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg
    18721 tgaagccctc aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt
    18781 gaatgtagaa ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa
    18841 atctacaatg ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat
    18901 cgaaggaaaa atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg
    18961 ctatgaaaat atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga
    19021 aatggtcgat gatatcattg aatttgctga catcggcgac catatttatc aacctgtaaa
    19081 aacctattca agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc
    19141 tgatattttg attgtggatg aagcattggc agtaggagat cttgaatttc aactaaaatg
    19201 tatggaaaaa tttacagaaa tcaagaactc gggaaagacg attttattcg tttctcatga
    19261 tattagttcg attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gaaaagttgt
    19321 cgaatatggt gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc
    19381 agttaaaaca gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt
    19441 agacgtgacg aaagccgaat tgttggataa agataaaaat ccagtggata tgctggaaca
    19501 aggagaaaaa ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc
    19561 agttttagga attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct
    19621 actagatggt atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga
    19681 aaagttaggt ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc
    19741 aacggttcct ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat
    19801 cggagaaggc atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat
    19861 atgattttga aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca
    19921 aaccaggaag taaagtttta gagtttggtc ctggaaacgg ccgattaacg aaacatttga
    19981 tcggagcaaa acaatgtgaa gtgagcattg tcgagctaga taaggaattg ttcgattttg
    20041 tcagcgagtt tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact
    20101 attatgctgg acaaacattt gattacgttt tgtttgccga tgttttagaa catttggtag
    20161 atcctggaaa aacgttgaag aaagttagag aatttttgaa tgaagaagga gaaattctaa
    20221 ttacattccc taaccttgcg cataattctg tcatgattga tttgttcaat aatcaattgc
    20281 catgggcttc ttatggtttg ctagatgaaa cccataattc tttttatact catgatggct
    20341 tccaaaaagt ttttgaaaaa gcaggattat acatcaatat tgaagattat ttgtacttag
    20401 ctgtaggtga tacagaatta aaatcaactt atgaagaatt gccggaagct gtacgttatg
    20461 atttcaaaat gcgaccattt ggcgaagtct accaatactt cttctcattg atgaaacatc
    20521 cagtagcaca aagttccatt gcgcagccgc aaaattctaa ttatgtgaaa gtattagaag
    20581 taatccagca aacgaaacaa gatgaaataa ttcaacaaat accattcaat aatttcacag
    20641 gggaaaatga gacactaagt ttccatgtat cagaattagc agaacgaatg gtgtttcgct
    20701 tcgcgcagca acctagcttt atcgaattca gcgcagaagc tgctggagaa aaactcacat
    20761 ttatcgattc aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatggtaaag
    20821 atttaccaga gttcttttta acggatattg cagggaaaga agtgactatc cattgtcact
    20881 atcgttttat cggagaattg acaccaacaa tgaaagaatt gctggaaaca atccgtccaa
    20941 tggctgaagt tacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcatt
    21001 atcttattga gaataataca agattagatc agacgttgaa aacaaccata aatcgctatt
    21061 gcacgttgtt ggaaagtgat gaatggacaa tcaaacatag attagggcga cgtaagaaag
    21121 aaacatcgaa aaaaatccaa gaaaaagagc tttctctatg tattgacgaa aaaatctggg
    21181 atgcagaaac aaagatattg aaaatcatcg ggtggggaat cgctaattcg gatcgccaac
    21241 cattgtccta taagttatca gccgaccaat caccattttt tgaagcaatt cctgttttac
    21301 gagaagaagt caatcaagca aaacagctag ctgaaggagc aaaagcagga tttgaactgc
    21361 ggatcctttg cgagaaggaa cgttcattct tagttgaagc agtggctcaa aatggtcaat
    21421 catggatcat tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt
    21481 gcaaaaaaaa gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga
    21541 tttgcttatt gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt
    21601 agaaaaagaa aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt
    21661 atataatctt gatgttaaga cccaaagcgg ttttaaggta cgtttatcag ggaaaatgag
    21721 aggaaaagca atcttagact tccaaacagc aagacatcaa aatggcatag ctgtgaaatt
    21781 aaacggtaaa tatccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt
    21841 actgaaaaaa gggataaatt atgcaaggac tcatggcgta aaaaaagcaa tccgccgggt
    21901 caaactggaa ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcatgagaa
    21961 acctgatctg aaagagcaaa ggaaagaagt acaaggattt gcctaccgcc cattgatttc
    22021 catacttatg cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt
    22081 acttgatcaa acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc
    22141 agctatccga aagtgtctag aatcctatca agcaaaagat gatagaatta aagtcgtttt
    22201 ccgacaagaa aatggacaca tcagtctggc aactaattct gcattggaaa tggcagaagg
    22261 agagtttata gcgctcttag ataatgatga cgaattacct ccatttgcct tatatgaagt
    22321 ggcaaaagtg ctaaacgtac atccagaact ggatttgatc tacagtgacg aagataaaat
    22381 cgatgcagat gggaatcgct ttgacccgca cttcaaagca gactggtctc ccgatacatt
    22441 gatgggaaat aattatatct ctcacttagg cgtatatcgg acaagcattg ttaaggaact
    22501 tggcggtttc cgtaaaggat atgaaggctc tcaagattat gatttggttc ttcgtgtgac
    22561 agaacaaatt cctgcagaac acatttacca cattgaccgt gtcttgtatc attggcggac
    22621 gatcccggga tcaactgcaa gcaatggaga agctaaaagc tacatctatg attctggagt
    22681 gaaagcattg acagatgctc tatctagaag aaatatcaag ggaagcgtac atcctggcag
    22741 aatttctggt ttttatgaaa tcacttatga tgttttgcaa gaagacttgg tcagtgtgat
    22801 cattccaact aagaatggct atgaagatct aaaaacttgt gtggattcga tcatcgaaaa
    22861 aacgtcttat ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat
    22921 gcaagaatta tttgcagaat acaagcatca gctgaaagac cgatttatcg tcgaattaat
    22981 tgacattcca ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa
    23041 atacttttta ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat
    23101 ggtttcttat gcacagtttg atcgaatcgg ctgtgtagga gcaaaattat tttatccgga
    23161 tgatacaacg cagcatgcag gtgtattatt agggatcggt ggcgttgcag gacatgcatt
    23221 gaataactat gatcgaacac attgtggtta ctttggccga ttggtcattg acgtcaatta
    23281 cttagcagtc actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg
    23341 atttgatgaa atattagaag tagcattcaa cgatgttgac ttgtgcttga aagtttatga
    23401 actgggtcgt tacaatgtct atgcacatca agcagaattg taccatttcg aatctaaatc
    23461 aagaggttat gaagatacac cagaaaaaca aagacgtttt gctggggaaa tcaaaaaaat
    23521 gcaagataag tggccttctt atattgctca tgacccattt tataacgata atctaactaa
    23581 agaaggaatc ggcgattttt cgttgagacc tgattgatta aaagaactgt attttcattt
    23641 ttattaatga aaatacagtt cttttaattt aatt
//
LOCUS       DACEOM010000049        23576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEOM010000049 DACEOM010000000
VERSION     DACEOM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.050"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IW781_001844"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IW781_001844"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1182843.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IW781_001845"
     CDS             complement(1174..1869)
                     /locus_tag="IW781_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1182844.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IW781_001846"
     CDS             complement(1882..2598)
                     /locus_tag="IW781_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1182845.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IW781_001847"
     CDS             2928..3533
                     /locus_tag="IW781_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1182846.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IW781_001848"
     CDS             3858..4181
                     /locus_tag="IW781_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1182847.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IW781_001849"
     CDS             4363..5733
                     /locus_tag="IW781_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1182848.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IW781_001850"
     CDS             complement(5863..7164)
                     /locus_tag="IW781_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1182849.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IW781_001851"
     CDS             complement(7394..9385)
                     /locus_tag="IW781_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1182850.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IW781_001852"
     CDS             complement(9529..9795)
                     /locus_tag="IW781_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1182851.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IW781_001853"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IW781_001853"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1182852.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IW781_001854"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IW781_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1182853.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IW781_001855"
     CDS             13139..13702
                     /locus_tag="IW781_001855"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1182854.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IW781_001856"
     CDS             complement(13955..14476)
                     /locus_tag="IW781_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1182855.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IW781_001857"
     CDS             complement(14479..15321)
                     /locus_tag="IW781_001857"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1182856.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IW781_001858"
     CDS             complement(15314..16039)
                     /locus_tag="IW781_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1182857.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IW781_001859"
     CDS             16215..16511
                     /locus_tag="IW781_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182858.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="IW781_001860"
     CDS             17079..18671
                     /locus_tag="IW781_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1182859.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="IW781_001861"
     CDS             18684..19424
                     /locus_tag="IW781_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182860.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19592..20941
                     /locus_tag="IW781_001862"
     CDS             19592..20941
                     /locus_tag="IW781_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1182861.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21069..21659)
                     /locus_tag="IW781_001863"
     CDS             complement(21069..21659)
                     /locus_tag="IW781_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1182862.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21806..23158
                     /gene="brnQ"
                     /locus_tag="IW781_001864"
     CDS             21806..23158
                     /gene="brnQ"
                     /locus_tag="IW781_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1182863.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6754 a         4746 c         4491 g         7585 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    19501 tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta
    19561 attaaacttt tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg
    19621 actgaaaaat agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt
    19681 tttaggttcc ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat
    19741 tactggatgc atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac
    19801 caataaccat tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat
    19861 cactggctgg acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat
    19921 cggaatgtat gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc
    19981 attaggtctt cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt
    20041 ttggtttgca ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat
    20101 gatcctcacc cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca
    20161 tggcggattc tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt
    20221 tgcttttgct gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa
    20281 agtgttgccg gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc
    20341 tctttttgtc attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt
    20401 tgtagaagta ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt
    20461 attaagtgca gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg
    20521 ttctttagcg caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt
    20581 gccagggaat gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta
    20641 cgtgatgcct agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt
    20701 tatctggagt atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa
    20761 agctcattca tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat
    20821 ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac
    20881 acctgtgtgg ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta
    20941 agaaagtagt tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt
    21001 gcttcttaaa tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag
    21061 cctcttttct atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa
    21121 aaaatccaaa taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag
    21181 acaccggcaa tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac
    21241 aaaactctca cgacatggga atctggttta tttgctttca tgatttgcct catcaagata
    21301 ccaaaagaaa aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct
    21361 tcagggttac gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa
    21421 aagatattcg accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga
    21481 tggttcaccc agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt
    21541 cggatgaccc ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaaga
    21601 actctcaaaa caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg
    21661 atgattcctc ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca
    21721 tcgttttata tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt
    21781 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    21841 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    21901 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    21961 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    22021 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    22081 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    22141 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    22201 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    22261 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    22321 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    22381 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    22441 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    22501 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    22561 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    22621 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    22681 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    22741 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    22801 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    22861 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    22921 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    22981 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    23041 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    23101 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    23161 aaagaggttg tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc
    23221 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt
    23281 attgagaggt cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg
    23341 gcttctcaca tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt
    23401 ttattgagcg tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca
    23461 tatactttgc cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct
    23521 tttataatat agattttaga catatatatg atataaaaat agttttcgca aaaaaa
//
LOCUS       DACEOM010000050        23555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEOM010000050 DACEOM010000000
VERSION     DACEOM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.051"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(125..895)
                     /locus_tag="IW781_001865"
     CDS             complement(125..895)
                     /locus_tag="IW781_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1182864.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(888..1823)
                     /locus_tag="IW781_001866"
     CDS             complement(888..1823)
                     /locus_tag="IW781_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1182865.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2417..4066)
                     /locus_tag="IW781_001867"
     CDS             complement(2417..4066)
                     /locus_tag="IW781_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1182866.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(4261..5157)
                     /locus_tag="IW781_001868"
     CDS             complement(4261..5157)
                     /locus_tag="IW781_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1182867.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(5150..5830)
                     /locus_tag="IW781_001869"
     CDS             complement(5150..5830)
                     /locus_tag="IW781_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1182868.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(5831..6613)
                     /locus_tag="IW781_001870"
     CDS             complement(5831..6613)
                     /locus_tag="IW781_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1182869.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(6606..7523)
                     /locus_tag="IW781_001871"
     CDS             complement(6606..7523)
                     /locus_tag="IW781_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1182870.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(7526..7810)
                     /locus_tag="IW781_001872"
     CDS             complement(7526..7810)
                     /locus_tag="IW781_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1182871.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            8598..11825
                     /gene="fss3"
                     /locus_tag="IW781_001873"
     CDS             8598..11825
                     /gene="fss3"
                     /locus_tag="IW781_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1182872.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGDKAQGKAVFEGTEYTL
                     FTAKDGKAVKWSEAFKPEMVKGTKASDETVTLALDEKNQAAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQIIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVNCTVERHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIEYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGTINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSLTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            11938..12252
                     /locus_tag="IW781_001874"
     CDS             11938..12252
                     /locus_tag="IW781_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1182873.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            12265..12639
                     /locus_tag="IW781_001875"
     CDS             12265..12639
                     /locus_tag="IW781_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1182874.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            12649..12993
                     /locus_tag="IW781_001876"
     CDS             12649..12993
                     /locus_tag="IW781_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1182875.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDIPDWLGEEESFFMVVEVNESLCRQAESVLEEIGVEMPDAIEGFLKQL
                     VETKQLPVVVND"
     gene            13062..14078
                     /locus_tag="IW781_001877"
     CDS             13062..14078
                     /locus_tag="IW781_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1182876.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLSALFIGWMGVFYFPELRQFGL
                     SKVSFSTIEIAWSMKDLVCLLGSLALSGSMMLLYIHFFPDHWRSLWNRQKLARMILEN
                     HWYEVKQTQSDGFFKDLNRSRTKETISYFPKVYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISKCVEDFYERMMARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            14672..16546
                     /gene="ltrA"
                     /locus_tag="IW781_001878"
     CDS             14672..16546
                     /gene="ltrA"
                     /locus_tag="IW781_001878"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1182877.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            16618..17028
                     /locus_tag="IW781_001879"
                     /pseudo
     CDS             16618..17028
                     /locus_tag="IW781_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            17073..18220
                     /gene="dcm"
                     /locus_tag="IW781_001880"
                     /pseudo
     CDS             17073..18220
                     /gene="dcm"
                     /locus_tag="IW781_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            18210..18499
                     /locus_tag="IW781_001881"
                     /pseudo
     CDS             18210..18499
                     /locus_tag="IW781_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18755..19939
                     /locus_tag="IW781_001882"
     CDS             18755..19939
                     /locus_tag="IW781_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1182878.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            19954..20049
                     /locus_tag="IW781_001883"
     CDS             19954..20049
                     /locus_tag="IW781_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1182879.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            20078..20299
                     /locus_tag="IW781_001884"
     CDS             20078..20299
                     /locus_tag="IW781_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182880.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            20312..20815
                     /locus_tag="IW781_001885"
     CDS             20312..20815
                     /locus_tag="IW781_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1182881.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            20941..21978
                     /locus_tag="IW781_001886"
     CDS             20941..21978
                     /locus_tag="IW781_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182882.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            21993..>23555
                     /locus_tag="IW781_001887"
     CDS             21993..>23555
                     /locus_tag="IW781_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAQ1182883.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDKENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSW"
BASE COUNT         7599 a         4269 c         4773 g         6914 t
ORIGIN      
        1 cactaaacgg tgttcaatca gctgaccctc ggtattcgct caaaaaacaa caaaatatga
       61 ggaactattt tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
      121 ttttctaact tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa
      181 caacgatgaa taacacaagt acgatcatcg atgttgctac tggcacatcg atcgttccaa
      241 ggaatccgta acggaaaccg gagatcatat aaacgatcgg attgactttt gaaacagctt
      301 gccaaaaggg cggtaacata gagatggcat agaaaacgcc acctagataa gttaacggct
      361 gcaagacaaa tgtaggcacg atcgatacat catcataaga ctgagcaaaa atcccgttga
      421 gcagtcctgc taatgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc
      481 atgaatacac atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga
      541 gaatactgcg acccacacca ccaatcacaa agccccaaat aatcacatgc gtaggaacag
      601 gtgctaccaa gatttcttcg atatttttct gaaatttttg agagaagaag gacgaagaaa
      661 cattggcata cgaactagtg atagcagaca tcataatcaa accaggaacg atgaattcca
      721 tatatgaaaa accacccatg tcaccgatcc gcccaccgat catttttccg aaaataacaa
      781 aatacaagga tgtcgtgatc actggcggaa ccagtgtttg gacccatata cgcaaatagc
      841 ggttcgtttc cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc
      901 tcctccgtat gattattttc ttctgtgatt ttcaagaaca gttcttctaa acgattcgac
      961 ttattcctca tagaaagaac tttgattcct tgtcttgtaa gctgatcgaa gattccgttc
     1021 accccttgat ttcgttcaac ttcgacagcg attgtctgct catcttcaaa tgtacaagca
     1081 tatccttcaa tcacaggttt ggtatcatat ggcgctaaat caaagataaa tgtttcaaat
     1141 tgcagttttg acagaagctc tttcatactt gtattctcga tcagttcacc cgattggatg
     1201 attccgatgt tgcgacaaag catttcagct tcttctagat aatgcgtcgt caaaatgatc
     1261 gttgtaccat tttcgttcaa ttctcgtaga aactcccaca tctcgcgtct caactcgatg
     1321 tcaactcctg ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta
     1381 gcaatcatca gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt
     1441 tcccaaagat tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag
     1501 acaccgtagt agcctgcttg attcacgacg atttgctgga ccgtttcaaa agggttaaaa
     1561 ttgaactctt gtggaaccaa tccaatctgt tgctttgctc gaactagatc cttgtccaga
     1621 tcataaccga agacattcac ttttccagat gttttattga ctaaagaagt aataatcccg
     1681 atcgttgtag acttcccagc tccatttggc cctaatagcg catagaaatc tccttctttc
     1741 accgaaagat cgattccccg caatgcttcg accccacttc catagatctt tttcaaatta
     1801 tttatttcaa gcgcatttgc catttatttc ctatctcctt atagacatga ttcttaattg
     1861 tatcatggaa tacagcagat gaaaaaacaa cttggcttcc acacaaaaac acacctgcta
     1921 acaaagagga gatgtgtttt ctagttcatg atcatcttcg atagaataac tatctaaaca
     1981 actctggaac ttttaaaatc ataaaagtac cgacagcaac tactacgatg acatatagaa
     2041 gatccttata actgaggttc ttgtcttttt tattcatgtt aagctttcct ccgtcctttt
     2101 atcgaaagct tttttctttt attttaaccg aaataaataa aatggaaata gttattttca
     2161 atttctgttt tcgctgtttg ttttacggag aactttgcca tggaacgtgc gtctcgtcgc
     2221 tgatgctaag ttttttcgat aaaagctgct agaaggaaga cagcgtggtt tattttgtat
     2281 cgtcttttgc taaatcttcc aagatcatct ggacagcaga ctcgttccca ttgaattctg
     2341 ccactttttt ggcatcaatg tacttctttt gataatcgtc tttttggacc cacgcatcat
     2401 gcttcgtttg ataaagttaa tcaaacggat accactgacg ctgttcatcg aacaacaaac
     2461 tttctttttg ggcctgttca actatggatt taggataatt caaaactttc agcaaggcaa
     2521 tcgcatttcg ggtccttgaa ggtccttctt ttaatttgaa atcaaaactg attccttttc
     2581 cttctgttac ttgttcttca aagtggacat tctcacaata attctttaaa atctctgtca
     2641 attcgatatc atgtgtggcg acaaatgcta aagatggata ctccgcaagc caccgaacga
     2701 ctgaagcgga agaagcaatc cgttcgatcg tatttgttcc ttttaaaatc tcatctacga
     2761 agcagtagca gcgttctttg gaagccactt ggtccagcaa acgtttgatc gatttgattt
     2821 ctgaaacaaa ataactgtcg ccttcaaata aatcatcttc gattgccatg gaagtcaaca
     2881 catgtccagg ttgcagggta aactgttctg caatcgctgt ttggatcgtc tgtgctagaa
     2941 tacagctgat ggctatactt ttgacatacg tagattttcc tgatgcattg gaacccgtga
     3001 ctaatgtatt cttctgccag ttgactggat taactaccgc ttcttttaac aatggatgat
     3061 agatatcttt cgctgtcaca ccgccctcag taaactctgg tcggcaagtg atcggcatat
     3121 aggtgcgata attcaaaacg gctgctgcaa cttccaattc tcccagcaat tcccacagct
     3181 tgatggcttc agttgaatgc ttttctaatt tagtgagaac gaaatgatag gagataaatg
     3241 gcagcatgaa catgatgttg acatattcaa aaatcaattc tgcttcactc ccgctcttca
     3301 ttcgaaatga aaaacctaat ttagggatgt ctcttaatgg tttagtttgc tgtttcagct
     3361 ctttttgaag tggtgtcgtc atttttgcta actgattggc ggctgaaatc gttgaaacca
     3421 aataactcat gctattcagc tctgtttcta gtgtttgttt cttcactaga taatagaccg
     3481 tattgaatac taagccaccg attgctagat agattcctag tacttgccct aaaagaagca
     3541 aacctacacc caccagcgga agcaagccaa gaaaaataaa taaacctacg cgtccaatct
     3601 ttttactttt tccatccgct agatattgtt ttgcaaaatt atgatctttt tttcctaaac
     3661 gagcgaattg atattggatt ctttctctca gttgaggatt ttcctgataa aaagcaatta
     3721 gtttttctaa ctgataatct tctccaaaat caaagctacg taaacgctga tacaacgctt
     3781 ctgaacctac actagaatag gtactgttga tcccttcaaa tacttcgaac atatccaagt
     3841 cataccaagt aagatcgtcg atttcactat cccatgaacg gaattttttc tccgtgagcc
     3901 acgcatcctt caaactttct tctttatcaa atctcggttg ataaggaatt tttccccaat
     3961 tactacgaac tgtttccttt actttcaaac gtgaaaaaat gtctaaaaca actaataaaa
     4021 taacaactgc aataataatt aaaacgataa attctctttg gcccatatcc ctctccgttc
     4081 cttgatttgt tagttttagt atagcactgt tgcgctattt gaaaaggggg tacaaaaata
     4141 actgtcttcg ttacctaatt cgtatcaatc acaccaaaat aaaaacacct tccaaaaaag
     4201 aagatgtctt taaaacgaga tacaattttt tgtcattcac ttcaggtaaa ttagagccaa
     4261 ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat
     4321 ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc
     4381 cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt
     4441 ttgcgctcct ggtttgatag gattggccac tgttaatcga atgaatgaat cctcttttga
     4501 aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag
     4561 cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa
     4621 cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac
     4681 ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc
     4741 aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt
     4801 taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt
     4861 aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt
     4921 ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaccc atgtattcag
     4981 tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata
     5041 ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag
     5101 gaagaaaata aaacttccca taaagataac aacgagaagc aacagccact taatcaaatc
     5161 taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct agtttccctc
     5221 gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa
     5281 ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca
     5341 gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg
     5401 actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga
     5461 cagaagtctg atgacgaagt tggatgccga ttcgtaacaa cagttcttcg atatcaaatg
     5521 gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc
     5581 tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg
     5641 cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc
     5701 ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa
     5761 gaacctcctc cagtaaagaa tgaacagctt catcgtcttc gataatcaaa atagtttcca
     5821 gcaggaacac ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata
     5881 ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt
     5941 gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc
     6001 ctttgatttt tgcgaaaaca ccgggaacga aggccgttcc catgatataa agaaaagaaa
     6061 gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg
     6121 tcgctaatat cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt
     6181 tgattccctc aacttcacca aatccgaaag caagtgtgta agcaatcatg gctagccccg
     6241 taatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat
     6301 aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt
     6361 ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt
     6421 ccaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat
     6481 tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca
     6541 tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta
     6601 atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc
     6661 ccaactaggt ggataccatc cacaggaact tttgcctcta ccaatgcttg attgatggct
     6721 gctacttggt caattccttc aaaaagctgg ataacacggc tagattggac aaaataatcc
     6781 ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt
     6841 ataaattgac gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct
     6901 tcatgcaaaa taccgtagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa
     6961 agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc
     7021 atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg
     7081 atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc
     7141 tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac
     7201 acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga
     7261 gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca
     7321 aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta
     7381 gacttacccg ctccattctc cccaatcaaa ccatagatca ttccacgttt gatttcgatc
     7441 gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag
     7501 accgtttcat ttccaaaatc catacttatc cttctttctt attgataagt gaagtatagt
     7561 tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa
     7621 tttcaagaaa aatcagactg tctcgcagac gtggttgagt tgtctgccaa tcaaagagcg
     7681 caccaaacag tgctaatcca agtggactgg ccaccatggt aatcgttgtt atcgttgaca
     7741 tcagacgtcc ttgtatatgc gttggaactt ctcgctgaag ccagaccata taaaaagcaa
     7801 aagaaaacat taatccttaa aaacggactt atgaaaaata atacatgccg tttgggattt
     7861 acaattagct accaaataag aggttatgac aaaagacttt tcacaatccc ttcttccgaa
     7921 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatttatc aaacatgaga
     7981 agcaattttt gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcag
     8041 aagcggataa ataatattgt ttgatgatac tttatctttt tcccgtttgt ttatagatcg
     8101 aatgatatta tagaccaaga atcctaccta ttaacatagc taaaaacaca aaaaaggcac
     8161 tttccaaatt gtggaaagtg ccagaaaagt taggattaag gtaactgcgc tcagcattgc
     8221 ttcgcccagt acgattctcg taactcgtga actcgaacga taatcgcaat taacgttttg
     8281 agaactgtga agctttacgg gctttcttaa gacctggttt tttcttcgta tcattgaata
     8341 agtttgaaaa gccttgattt ataaaggttt ttccgtaaat atattttgtt atatttgatt
     8401 aaatttgaaa tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat atttcgtatt
     8461 ttatattaaa tttttaggct cattcattct caaatccatt ctatttagtc aataaatatt
     8521 ttaaaaagac tttataaaca aagatgttca agcgagcatc ttattttttt gtgcctaatt
     8581 tgaaaggaga acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt
     8641 tgagtttggc tgttcaatca attgtaggag caattgctcc tacgattgct tttgctgatg
     8701 aaattaccca tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg
     8761 aaggaacttt tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt
     8821 acaacggcgt taaacaagat attttctgtg ttgaaccagg agtcccgatt tacaatgagt
     8881 tcactcctgg atatgagaag aacccgttac cagatatgtc cgataaagcc aaattagttt
     8941 ccgttttatg gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga
     9001 tctggcaaga agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg
     9061 taaatatcgc agccattgag gcaaaaatca atcaagccat tgccgattat cagaagaaac
     9121 caagcttcca taatagtacg gctaaaacag tattagggca atcgactact gtgacggata
     9181 cgaataatct gaatttgtca gagttcgatg aggttgtgga aaataccgca aacattgatt
     9241 atcgtgtgaa tggcaatcag ttgataatca gcccaaatgc caattcaaaa gaaagtggtg
     9301 tgttgacgct taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc
     9361 aacagacttt aatggccggg gcgatcgata agccgaacac ttatacggtc aaaattgatg
     9421 tggaaatgga gggttctttg aagattaaaa aagttgataa agaatcgggt gatattatac
     9481 caggaacggt tttccattta gactttggaa aaactttacc cgcaaaagac gtgaccaccg
     9541 ataaagaagg cattgcgacc ttagatgaga ttcctcatgg tacaaaggta accattactg
     9601 aaaaatcggt gccggcgcct tatacgattg ataccacacc catgactgcc acaattaaag
     9661 caggtgaaac catttcagtg acttcaaaaa atacgcgaga aaaaggtcaa attattctgg
     9721 ataaaaccgg aattgaaaca gggagtgatc tttggaacga taattactct ttagcgggca
     9781 atacgtttgc cattcgcaag gatagcccaa ctggtgaaat cgtccaagaa atgaccacgg
     9841 atgaaaaagg tcatgcagaa acaccaaaag agattgccaa tgcgttggaa ttgggaacct
     9901 actatgtgac tgaaaccaag gctagtcatg gctttgtgaa tactttcaaa ccagtaaaag
     9961 tcgaattgaa ttatgtcaat caaacagtgg ctcttgttac cagtaacgta aaagggcaaa
    10021 accaagaaat tactggtgaa accactttga cgaaagaaga caaagatact ggggataaag
    10081 cacaaggtaa ggcagtcttt gaggggactg aatatacctt gtttactgct aaggatggaa
    10141 aagcggttaa atggagtgaa gcttttaaac cagaaatggt aaagggaacg aaagcttctg
    10201 atgaaacagt gactctggct ttagatgaaa agaatcaagc tgccgttaaa cacttagcca
    10261 tcaacgagta ctactggcaa gaaaccaaag cacctgaagg atataccttg gatgaaacga
    10321 agtatcctgt ttctatcaaa aaagtagatg ataacgaaaa aaatgccgtg attacccgag
    10381 atgttacggc aaaagaacag attattcgtt ttgggtttga tttcttcaaa tttgccggat
    10441 cagccgctgg cactgccgaa actggcttca atgatctgac ctttaaagtg tcgccattgg
    10501 aagggaccaa tgaaatcaca ggtgccgaag atgaagcgac cacagcttat aacgagcaat
    10561 tagggtttga tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag
    10621 aagtagaagc cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta
    10681 aggaaaacaa agaagacttt gtgaagagtg agtatgtctt taccattacc gaacaagacc
    10741 aaaaacaacc aattaaaacg gtgaccgttc cttacgagaa gctgacgaac aaagcatttt
    10801 ctgttagttt gaaccgttta atgctctatg atttgcctga ggaagaagat agtttaactt
    10861 ctcttgcgac ttggaaagac ggaaacaaag aattaacttc tctggattct accgagctag
    10921 ttgataaatt gagttacaat ctacatgaaa tcaaagaaga ctggtatgtg gtagcgcaag
    10981 ccattgatgt ggatgcgaca aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg
    11041 tgattgcaga aacaagcgca acgttggcga acaaagagaa aacaggaact tggaaaattc
    11101 agcataaatt aaccgccgaa caagttttaa ataaaactat tgtcttattc aactatgtgt
    11161 atgaaaataa ggaagctttt gaagcaggcg ataagccagt agcgaaggat gttagcttga
    11221 ataatcaagc ccaaactgtt aattgtacgg tggaacgtca tgtttcgatc caaacaaagg
    11281 cccacctaga aaacggttct cagaccttta ctcacggtga tgtggtggat atgtttgatg
    11341 atgtgtcgat tactcatgat gtactggatg gctcaaaaga agctttcgaa acaattctgt
    11401 atgctttatt accggatggt acaaacaaag aaatttggaa atctggcaag attgagtatg
    11461 aagtaaatga taaagaattc accaaaactg tgcttgcgaa aaaagtagat accggaaaat
    11521 atccagaagg aaccaagttt acttttgcgg aaatcaatta cgataaagat gggactatca
    11581 atggaaaaca caatgaagat ttgaaagaaa aatctcaaac cttaacacca aaagaagttc
    11641 caaccatact aagtacgccg aaacaaccgg aaacaccaac tgttccaagt ggctctcaag
    11701 aatctagtct cacagtgaag acattccctc aaactgggga gaaaaattcc aatgtgttac
    11761 tgttcattgg ttttactttg atttttgcga cggcgggcta ttatttctgg aatcgccgga
    11821 actaaggtga tgagatgata caagtaaatc ctaagaacca acgtgcgcga ccgcctccta
    11881 aaaaagtagt cgcgctattt ttgtatccaa aactaacaat gaaaagagga aaagaagatg
    11941 gaattgaaat ttgtcgtgcc agatatggct gaaacctttg gaaagttaag ttatgccgga
    12001 gaaggcgaag ttttaacaga aggatatgga cggaatacta cggtgattgg tcgcagttat
    12061 catttgtatt ccagtaagca acgggccgat gatattgagg tggtagtagc tgcggaagcc
    12121 ggtgaaaagg attttgatca agaccaaccg ctaaaagccg tgaatcccca tttggttgcc
    12181 aaaggctatg agattgagaa tcgtgggttt actgattacg tgttgtatgt tgatgattta
    12241 gtgaaagcat aggaggaaaa gaagatgaga ttaacagaag gtattgtcgt agattcaggt
    12301 ttaacatttg ggaagttgcg tttttccgca ttacgccgag aagtacggaa gcaaaatgag
    12361 gatggaacaa ttggtaatga ggtaaaagaa cgaacctata atttaaaatc ctctgcacaa
    12421 gggcgaatga ttcaagttag tatcccggct aatgtaccct tacgcgagtt tgcttatgat
    12481 gcagaggtag agttggtcaa tccaatcgtg gataccgttg ccaattatgt ttttcgagaa
    12541 ggaactaccg ttaattggtt tattaaagca gatgatttag tcttgaagcg gcagccgaat
    12601 caaggaaatc ctacgaacca aaatgaaaga aagaaatagg tgaagattat ggaaattaat
    12661 gttgaacata ttttagattg tctcgatcaa tatggaaaag gtgagctaac ggaggaacaa
    12721 ctcactaacg cattaaccta tgatgaaaaa atgtttctga ttatgcatca agggttactg
    12781 gaagtaggta acgatgccaa agaagacttt gatataccgg attggttagg agaagaagag
    12841 tctttcttta tggttgtcga ggtaaatgaa agtttatgtc gacaggcaga gtctgtcttg
    12901 gaagaaatcg gtgtggagat gccggatgcc attgaaggct ttttaaaaca attggtggaa
    12961 acgaaacaac ttcccgtagt agtaaatgac tagcataact ttttgaaatt acccaatgaa
    13021 atcctattgg gctttttcga ctggaggtag aaatcatcaa aatgtacaaa ggacatcgca
    13081 tacgcgcagg agatcaacac ttgatttatc actttgttct tggctggcta agtgccttat
    13141 tcatcggttg gatgggcgtc ttttattttc cagagcttag acaatttggt ctttctaagg
    13201 tatcattttc cactattgaa atcgcttggt ccatgaaaga tttagtatgt ctgcttggaa
    13261 gtctcgctct ttcaggaagt atgatgttgc tttatattca tttttttcct gatcattggc
    13321 gaagtttgtg gaatcgacaa aagctagcca gaatgattct agaaaaccat tggtacgaag
    13381 tgaagcaaac tcaaagtgat ggctttttca aagatttgaa taggagtcga accaaagaga
    13441 ctatcagtta cttccccaaa gtttattatc gaatgaaaga tggcttactg tctattcgcg
    13501 ttcaaatttc actgggaaaa tatcaagatc agctattgaa gttggaaaag aagttagaaa
    13561 gcggactgta ttgtgagtta gtagaaaagg aactcaaaga ctcctatgtg gaatacacct
    13621 tgttatacga tatgattgcc aatcgaattg ggatagaaga agtagtggca gaaaatggga
    13681 cgttgcgatt gatgaaaaat caagtatggg cgtatgattc cttgcctcat atgttaatcg
    13741 ctggtggcac aggtggtggg aagacctatt tcttactcac cattattcaa gcactattga
    13801 agtcagatgc agaattgttt atcctcgatc ccaaaaatgc ggacctcgcc gatttaggta
    13861 cggtaatgcc tcatgtctat tctcaaaaag aagaaatttc taaatgtgtg gaagattttt
    13921 acgaacgcat gatggctcgt agtaggtcaa tgaaagaaat gcccaactac aaaacaggag
    13981 agaattatgc gtatctcgga cttccgccca actttttaat ctttgatgaa tacgtggctt
    14041 atatgggtgc gaatcgtttt cctacaagca tcgaatagat gtgaaatgga agcctattga
    14101 gattatcaat agtgacaact aatgttttga gagatggaat gaaagccttc aagttgagct
    14161 gaaacatccc cgctaatact cctacatgct ctaatcaatg aagtagattg gttttggtat
    14221 gaagctaggt gaagtcgtct gaaatctgcc taagtgcttg agcataaggc tgactatagg
    14281 gcgggcggct gaaaaagata actatggtaa gaatgtctga aaatgactga cgaatctgcg
    14341 aatgtacggg tctaaaaggc tgatcttatt gaaagatagg aacatacgga cgtatggtga
    14401 gataccgaca agtaaggttc catgatatga aaatcgggat agctaacgaa gaagctataa
    14461 tttcacaggc tcatagtaga tacctaaggt tatacatgat agctaaaaac attggaacga
    14521 tgtaagcaga aaactatcaa ttaaatgact acttctagat aggtgcatat aagtaataac
    14581 gaaattgctt tgtttctgtg aaagtaaggg cacagtaccg atgaagcgtc taacaaatgt
    14641 ggagggatag ccctaagtct atttaataac aatgaatgtt gaaacagatg aagttcaagg
    14701 atctggtaag aacgtgggaa aatccgttat ggaggtgcca cgcttgaaca caaaaatacg
    14761 ttactgggaa tattatgatc tacaagaaac attcgatcag ctatttactc aaagtagaaa
    14821 cgggaaaaag ttttatcaat tatatgagtt aatcatttct gaaaataata ttcttttagc
    14881 ataccgtact atcaaggcaa acaaggggtc ttcgacacca ggaacggatt ccttcacaat
    14941 tgataactat aaggaaatga accaagcaga gtttatccat cttattctca gtcatttaga
    15001 aaattacaaa ccaaaatcca taaagcgagt aatgatacct aaacccaatg gtgagaaacg
    15061 cccattagga attccatgca tgattgatcg aatgatccaa cagatgttca agcagatact
    15121 ggagccaata tgtgaagcta agttttatga acatagctac ggtttcagac cattgagaag
    15181 tgcaaagcat gctctaggtc gcattatgta tctcataaac atcagtaaaa tgcactatgc
    15241 agtagacatt gatatcaaag gtttctttga taatgtaaac caccgtttac tgataaaaca
    15301 attatggaat ataggaattt gtgacaaaca agtcttagct attctttcaa aatctctgaa
    15361 atcacccatt caaggagaag gtatcccgag caaaggaacg atccaaggag gtatcatttc
    15421 tccactatta tctaatgttg ttctaaacga cttagaccat tgggtctcaa agcagtggca
    15481 tacctttgaa acaaagtatc cctatactaa aggttacaat aaatttcgtg ctctaagaga
    15541 tacaaatcta aagcaaggct atattgtcag gtacgcagat gactttaaaa tcatgactaa
    15601 tgattatccg acagcattaa aatggttcca cgctgtcaag ctttacctaa aagacagact
    15661 taaattagat atttccaatg agaagtctaa aatcgtcaat ttgcgtaaat gtaagtcgga
    15721 atttttagga ttcgctattt gcgtgaaaca aaaagggaaa aaatgggttt gtaattcacg
    15781 tatttctaac aaaaaaaagg atcagattaa agaagagatt aaacaaagaa tcaaagatat
    15841 tcaaaagagt cctacagcac aaaatgctct cctgtttaat gctctcgtgc tcggtgttca
    15901 taattatttc aggtatgcca ctcatattaa tttagatttg aataggatga actacgttct
    15961 ttcattcact acttacaacc gattatcaaa ttgtagtaca agatgttacc ccaacaaagc
    16021 ttccccaact tacaaaaagt tttactcaac aacgatgaaa acgtataaag tagctggagt
    16081 ctatttgtac cctctttgcg acgtatctac aaaaacaatt tacggattta atacggagga
    16141 tacaccgttt actccgtttg gtcgtcaaag attggagcac aaaagcctac agtctcttgt
    16201 gttccaagaa ttaagaaaat tgatggaatc gaaaataccg aatagaacgg ttgagtattt
    16261 agatagtaga atatctcgtt actcaatgaa atcaggcaag tgtgagatta caaaacaatt
    16321 tttgccagca aaagccgttc actgtcatca ctatttgcca aaaagtcttg gtggagacga
    16381 caaattcgat aacttgagaa ttattcataa ggatatccat cttctgatcc atacaacaaa
    16441 taaaatgata atcgatcact acgtgcatga actgaaactg gtgccagagc aaatagcaaa
    16501 aatcaacctt tatcgaaaga tgtgtaatct gcaaaacatt caatagaaaa taaatagatg
    16561 gaacgccgta tgaggtgaaa gtctcacgta cggtgtggag cgggggaaaa gatggagatt
    16621 atatcaaatt cttacctatc gctataaatg ttaacaacaa aggaaagtgc ggtgattttg
    16681 aataagctca agcaaattgt gatgttaggt cgtcagtctg gtttctttct gattttggct
    16741 tgtcaaagac cagatgcgaa atatttagga gatgggattc gcgatcaatt caactttcgt
    16801 gtagctttgg ggcgtatgag tgagctcggg tattccatga tgtttgggga agttgataaa
    16861 aatttcttca tgaaacgcat caaaggtcga ggatatgtgg atacaggagg gagtgtgatt
    16921 agtgagtttt atacgccact tgtaccaaaa gggtatgatt tcttgaggga aattagcgat
    16981 attgttggat taaccgttca tacggaaaga gaaaataata gtgtgtagcc taggaatatc
    17041 aataatcctg ggctttttta ttgaggtgat ttatgacatt tttagattta ttcgccggga
    17101 ttggtggctt tcgcttaggt atggaacaag cgggtcacca gtgtatcggc ttttgttttg
    17161 tgaaattgat gaatttgctc ggaggagtta taaagcaatc catgatacaa ggaaggaggt
    17221 ggaaatgcat gacatcacaa gtgtatcaga cgaatttgtt cgatccctcg gaccagtgga
    17281 catcctttgc ggcggctttc cgtgccaagc tttttcaatt gcgggaaagc ggcaaggatt
    17341 ttccgatact cgaggtactt tattctttga aatcgctcgg ttcgccactc ttctccaacc
    17401 taagtttcta ttccttgaga acgtccgggg attgctcaat cacgaaggag gggctacgtt
    17461 cgagacgatc ctccgaacgt tggatggatt ggggtatgat gtggaatggc aagtgcttaa
    17521 ctcaaaggcc tacgttcccc aaagccgtga gcggatcttc cttatcggac attctcgaga
    17581 tgcatgtacc gaacaagtat ttcctatcat tggatcgtct tcaacatctg atcaaaacat
    17641 cagaaacttg gtaaatatca atccttccaa tcgaggaatg ggtggccaag tctatggttc
    17701 agatagcgta gcaccaacct tgaccggtga tgaaggaatt aaaattgcct taccggtaaa
    17761 ggatgggatt tctgtagcgg gtatgttgtc gggaaatttt gaacaaggca ataggattta
    17821 tgaggtaacc ggaacagccc ccactttgtc aacgaaacaa gggggaacga agattatgat
    17881 tcggaaaaat gaaacagaat atcaagaggt gaagccaggg gacagtgtga acctggcttt
    17941 tcctaattcc acaagtcgcc gaggtcgtct gggaaaacaa agtgtgcata ccttactaac
    18001 aggagaccaa caagcggtag tcacggatca gtaccagatt cgaaagttaa cacctaggga
    18061 gtgctggcgg cttcagggct ttccagattg ggcttttgat cgagcttctc aagtcaattc
    18121 agatagtcag ttgtataaac aggccggaaa ctcagtaaca gttccggtca tttttgatat
    18181 tgctagaaga ttaaaggggg taaaagcata tgatttatga cgagttgatt ggagaaatct
    18241 actgggtgat tggcaagatt caggcagatc cgaaattgga agaagaactg taccggctca
    18301 attttgatat tcgcaaaaac ggggtgaaag tccaggtgat tcgtatgtag tggatgagga
    18361 aacggatgct cgaatggagc tcaatcaagt catcggtgaa ttggaacgaa ttgcggacct
    18421 tgccaaagaa cctgatatcc gccaatactt gtttgagata aaagcagagt tggaaattga
    18481 gggtatcacc gcggagtaat agggaaataa cccactgata ggcactgacc gcgggggatt
    18541 tgcgaagcag aaatcctccg tggtgctgtg cctgttcatg gacggcggca gccggccatg
    18601 atgtctcacc ccccgtatct aacagggggg tacaaaaaca caaaaaacaa gcaagtaacc
    18661 ataaaaaatg cttgttgtat caaggatatt aagtaatttg tataggtgtt aactcatttg
    18721 gaaaagttaa caccttgaat ttggaggtga aaacttggcg caacgaaatt tagattatcg
    18781 gttattaaaa gaacgacgca acgaatatgg cgtttcacaa aataaattag ctactgcttg
    18841 cggacttagc cgcccatacc taaatcagat agaaaacggt ggcgtaatcg cctctacaaa
    18901 gaccatgaga aaaatttttg atcaactgga aagtttcaat cccgatttac ctttaacgtt
    18961 actatttgac tatgtaagga ttcgctttcc cacaacggat gtgcggaaaa ttattcaaga
    19021 aattctccat ttgaaattcg agtatatgct tcatgaagat tacgcctttt actcctatca
    19081 agaacaatat gtcatgggcg atattgtagt gatgttatct catgaagaag ataaaggtgt
    19141 ccttttggaa ttaaaaggtc gtggttgccg gcagtttgaa acttttttac tcgcccaaaa
    19201 gcgtagctgg tacgactttt ttgaagattg tctaaaagcc ggtggcgtga tgaaacgctt
    19261 ggatttagca atcaatgatc gagtggggct actggatatt cctgatttaa cgaaaaaatg
    19321 tcagaaggaa gaatgtatct ccttgtttcg taccttcaaa agttaccgtt ccggggaact
    19381 gttgaaagct gatgaaaagg atggtatggg caatacctta tatattggca gcctcaaaag
    19441 cgaggtttat ttttgtttgt acgaaaaaga ttatgaacaa tatatcaagt tagggattcc
    19501 attggaccag accgaaacga aaaatcgttt tgagattcgg ttgaaaaatg accgtgctta
    19561 ttatgcgatt caagatttgt taaaaggtcg tagcattgaa agtaccacgt tttccattat
    19621 caatcggtat ttgcgatttg cggataaagt agatggaaaa cggcgaacca attggccctt
    19681 gaatgaacaa tggggtcgtt ttattggacg gaatcggaaa gagattcaat taacttctga
    19741 accgaaaccg tataccattg aacgaacatt aaattggcta ggtagacaag tcgcccctac
    19801 gtggaaaatg gctaaggagt tggatcgatt gaatcaaaca acgtatatcc aggatatggt
    19861 acagaatgcg cgactctcgg accggcataa aaagattttg gagcaacaaa gtatggcaat
    19921 cgaaaacttg attgtatgaa aagaggaatt ttgatggaaa ttgtaaaaga tatgttattg
    19981 gtacttggtg gcagtatgct tggtgttacc gtgatgtgct tgatgaatgc aagtgcagca
    20041 gcggattgag caatggaaaa gaaaggaaag tgacaaaatg aatttcggac aaaatttata
    20101 tcaatggttt ttaaccaatg ctcaatcttt agtcttacta gcgattgtgg tcattggttt
    20161 gtttttagga tttaaacggg agttttccaa actgattggc ttcttggtga ttgctttgat
    20221 tgcggtaggt ctggtcttta atgcttctgg tgtgaaggat gtcctactca atttgtttaa
    20281 ccgaattgtc ggtgcctgag ggggaatcac aatggaacag atgcgtgttt atattgcgaa
    20341 ccttggaaaa tacaatgaag gtgagttggt gggggcatgg tttaccccac ctgtcgattt
    20401 tgatgaagtg aaagaacaga ttggattaaa tgacgaatat gaagagtacg cgattcatga
    20461 ttatgaactg ccttttgaga ttgatgagta tacgccaatt gaagagatta atcggttgtg
    20521 taacttagcg gaggaactag aaggaacgcc aattggtgaa gctgcttctg agattcaaca
    20581 cgctttcttc aattctttcg aggaaatggt agagcatgtg gatgatattg tctattatcc
    20641 agattgcgat gatatgagtg atgtggcgca ttacttgatt gtagaagctg gtgattttgg
    20701 cgaagttcca gaacaattga agccgtatat tgattttgat gcttgcggtc gagatttgga
    20761 gattggagga aattatttgg ttactagtcg tggagttttt gagtatccag aatgaatcaa
    20821 cactatatat agtgtttgat tagtatatgt aagcaaaaaa acacaatata tagagtgcaa
    20881 actgaagggt aatgtgatat aatatttgcc gaagaaatat ttttgaaatg aggtaatatt
    20941 atggcaaata ttcaaacaag ttttattgat ttccataatt caattagatt ggacgtagaa
    21001 gacaatacgc tacttaagga ctataaagac caagtaattg atggattaaa agattactta
    21061 cctgatgatg tgaagtttga aactttttta cagggaagct attctgttta taccggaata
    21121 aaaagctgtg atgaaaaaat tgattttgac atcgatattg cagttgcttt cgaaattgat
    21181 catactgttt atgaagatcc aagggagcct aaactgtggg taaaagaggc gctagttgaa
    21241 atttttccta atgctcaagt taatttaaaa gtaccttgtg taactgctac atttactgga
    21301 aaaaagacaa aaaaaaatgt gcacgttgat gttgcggttt atgctaaaga agatgaaaat
    21361 tatttcttag caaaagcaaa agaattcagt gctcccgaaa accgatgctg ggaggaagcg
    21421 gatcccaaag tattaaaaga gaaaattaat agtcatgtag cagattcaga tgatcgaaaa
    21481 caatttcgaa gatgtattcg ctatttaaaa agatggaaag ataacaattt taaccaagaa
    21541 tataaaccaa caggaattgg acttacaatt aatgtgatgg atacgttcct agttaataag
    21601 tccactgatt ttttaactag aaaagttcag tataatgata tggaatgtat gaagcagatt
    21661 gtttcttcgc tgaaagattc atttgtgtat gagtacagtg aaacagatgg ttggcattat
    21721 cgtctacatg catatcttcc agtgaagcct aacagtgaca cctattcaaa aatgacagtg
    21781 aatcagatga gtgattttaa aaacaaactt tctaaattat atgatgattt gattttcgct
    21841 atcgatactg aagatgagta tgaagctaca aaacgattaa ataatcaatt tggggaggat
    21901 tttttaatta tttcagaaga agaagttaca gaaaaaaact taagaaatgc atttgttaca
    21961 gactatccga gtgcttaggt tggtgttaaa gattggatta tactgatatt ttaaaagaaa
    22021 agctaacaga tgttgtgcgt ttattcgatg gtaattacta tggggatttt tcatttgcta
    22081 caattggaaa gttgcgttta aaaattgaaa taccaaaaca ttttccattg gtacttccta
    22141 ggatttacat tcataatttt gatcatttag gactttacct accacatgta gaaaaagatg
    22201 ggttggtatg tttttcttca gatataaatt atttagtaga taaagaaaat tcagaattgg
    22261 ttttattgca gtgtgttaat aaaactataa aaacaattga ggaaggtttg aaagggttaa
    22321 ataaaaatga ttttcgaaaa gagtttgttg ctttttggga caaacaagag aatcaaattt
    22381 cgttattcta cctttgtgaa ccagcgttgt ttatcaagtc agttgataca ataatgtacg
    22441 gtaagtcttt ggttgctttg gataaagaga acgaattaca attaaatgca gcgagtagat
    22501 tgtttaatgg agagaaagaa gatactcatg aattagatgc atactatgtt ccaattagaa
    22561 acaagaataa tattatgccc ccgaagaata ataaagcatg gtcaaagaag gatattttac
    22621 aaatagtaaa aaaaaatgta acaggtgcaa ataaaagatt ttttttaaaa cttgaaaaaa
    22681 gaaagaatag acataatgtt ttacttattc tagatatccc aattgaagaa aagtcaaata
    22741 atgtcataat tggatttctt tttacaaaac aatctggatc tataaaagat tataagggta
    22801 cgcctaggac tgtattagtt cagcgtcatg atgaattata tttggtgaat agaacgagcg
    22861 gagaaaacta tggattgggt gctaatatag ctattgttgg ggtagggtca gttggcagta
    22921 aaattgctga aaatcttggt ccattaggtg tgagaaagat gacactcata gacaatgatt
    22981 gttttcaagc tgaaaatttg tatcgaaatt cacttggtgc agatagttta aaaaatgccc
    23041 gatctcagaa taattttttt aaagttgatt taatgcagga ggagctcgaa aaaaaattcc
    23101 catacctttg tgttgaagtg gagtcaatga acgttttaga cttaattagt atgcaacctg
    23161 gttttttcaa ggaatatgac tatgtttttg tttgcatagg agatacaatg gcaagcctgg
    23221 ctttaaataa ttatttttcc aaaagtagtc aaaaagtttt ctattcttgg ctggagccat
    23281 atggcattgg gggacacact ctatttataa attattctct aaatcaaaaa ggttgtttca
    23341 attgtcttta tacagattca aactctaaaa ataggtgttc aaatagagct tctctggtaa
    23401 aagaaggaca aatttttgaa aggactttag cgagttgccg ctcaaaattt ataccatata
    23461 atgctattgc ctcgacggaa tgtgcaatta atgcaataaa acttttccat aaagtcaatc
    23521 aaaataatat gaccaatagt actctttttt catgg
//
LOCUS       DACEOM010000051        23485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEOM010000051 DACEOM010000000
VERSION     DACEOM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.052"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(341..1837)
                     /gene="lysS"
                     /locus_tag="IW781_001888"
     CDS             complement(341..1837)
                     /gene="lysS"
                     /locus_tag="IW781_001888"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1182884.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1948..2952)
                     /gene="dusB"
                     /locus_tag="IW781_001889"
     CDS             complement(1948..2952)
                     /gene="dusB"
                     /locus_tag="IW781_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1182885.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2953..3840)
                     /gene="hslO"
                     /locus_tag="IW781_001890"
     CDS             complement(2953..3840)
                     /gene="hslO"
                     /locus_tag="IW781_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1182886.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4057..6168)
                     /gene="hflB"
                     /locus_tag="IW781_001891"
     CDS             complement(4057..6168)
                     /gene="hflB"
                     /locus_tag="IW781_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1182887.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6258..6803)
                     /gene="hpt"
                     /locus_tag="IW781_001892"
     CDS             complement(6258..6803)
                     /gene="hpt"
                     /locus_tag="IW781_001892"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1182888.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6803..8248)
                     /gene="tilS"
                     /locus_tag="IW781_001893"
     CDS             complement(6803..8248)
                     /gene="tilS"
                     /locus_tag="IW781_001893"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1182889.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8368..8838)
                     /locus_tag="IW781_001894"
     CDS             complement(8368..8838)
                     /locus_tag="IW781_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1182890.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8884..9309)
                     /locus_tag="IW781_001895"
     CDS             complement(8884..9309)
                     /locus_tag="IW781_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1182891.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9376..9645)
                     /locus_tag="IW781_001896"
     CDS             complement(9376..9645)
                     /locus_tag="IW781_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1182892.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9656..11251)
                     /locus_tag="IW781_001897"
     CDS             complement(9656..11251)
                     /locus_tag="IW781_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1182893.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11266..14787)
                     /gene="mfd"
                     /locus_tag="IW781_001898"
     CDS             complement(11266..14787)
                     /gene="mfd"
                     /locus_tag="IW781_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1182894.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14804..15364)
                     /locus_tag="IW781_001899"
     CDS             complement(14804..15364)
                     /locus_tag="IW781_001899"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1182895.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15717..16691
                     /locus_tag="IW781_001900"
     CDS             15717..16691
                     /locus_tag="IW781_001900"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1182896.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16861..18261
                     /locus_tag="IW781_001901"
     CDS             16861..18261
                     /locus_tag="IW781_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1182897.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18339..19061)
                     /locus_tag="IW781_001902"
     CDS             complement(18339..19061)
                     /locus_tag="IW781_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1182898.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19140..19646)
                     /locus_tag="IW781_001903"
     CDS             complement(19140..19646)
                     /locus_tag="IW781_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1182899.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19769..21568
                     /locus_tag="IW781_001904"
     CDS             19769..21568
                     /locus_tag="IW781_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1182900.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21636..23210)
                     /locus_tag="IW781_001905"
     CDS             complement(21636..23210)
                     /locus_tag="IW781_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182901.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6435 a         5077 c         4268 g         7705 t
ORIGIN      
        1 aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg ttagtaacat
       61 gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt tggacaagta
      121 ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt caagtttttt
      181 tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc ttctttaatg
      241 tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt tctacacagc
      301 aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt gttgggaata
      361 gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg tcgatcccga
      421 tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag tcttcgtcta
      481 caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg aatctttctc
      541 tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc cccactatga
      601 atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct aaaggcgaaa
      661 cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca acaaatgttt
      721 caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct acattgtgtt
      781 tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct acaccagttt
      841 gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag tcgacagctt
      901 gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta cggataatac
      961 cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct tctaacatcg
     1021 taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg ccgatttcat
     1081 aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc gcaatacgca
     1141 aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg gcagctccac
     1201 ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca tctaaatagc
     1261 gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt tcgcgattac
     1321 tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag ccatggtatt
     1381 tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct ttgatagaaa
     1441 cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct ccaaggtctg
     1501 catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca taaatttgaa
     1561 tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct cggcgagtca
     1621 tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct tttgttcttg
     1681 gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt tttccaaatg
     1741 gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc atttgatcat
     1801 ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca ttctcatgtt
     1861 aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt ggaatttcgg
     1921 cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta ctttttccgc
     1981 tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg tctgattgat
     2041 tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg catgtgttct
     2101 gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta aatgaagttt
     2161 agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttctcctg tttcgagata
     2221 atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa tcatcacacc
     2281 gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga catccccgtt
     2341 ccccataaat gggatcgtca aatgtttttt cacttctttc aatacttccc agtccgcttt
     2401 tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg ctccagctga
     2461 ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac caatacgcat
     2521 cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt cgtatacttt
     2581 ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat ttactggaca
     2641 gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt gagcagcttc
     2701 aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt ctgtttcttc
     2761 aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt cactgatcat
     2821 ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg ctgaatttgt
     2881 tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc caattttcca
     2941 tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta attcttcttc
     3001 gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt gatcttcgtc
     3061 tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa atttttcttt
     3121 tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt cttctgtacc
     3181 aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg tagagattct
     3241 tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg cacctggcat
     3301 gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga ccaatacact
     3361 cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa aatcttcagc
     3421 gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc ctaagtcttt
     3481 tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt taccttcact
     3541 atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc cctttccatt
     3601 tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag ttagtttgtc
     3661 ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc ccaaagcagc
     3721 tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat ttgttgcttg
     3781 aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat aatctttcat
     3841 ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag caactactgc
     3901 ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag aaaacaggac
     3961 atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt gttccggttt
     4021 tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat cgtctgaatg
     4081 attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct cattcagttc
     4141 atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt ctttttcttc
     4201 caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat aatcatttga
     4261 ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt tggcatccaa
     4321 tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat gagcttcgat
     4381 gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt gatcaatctc
     4441 gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa caaacacttg
     4501 atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt ctgttaccat
     4561 gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg attggacatt
     4621 gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt caaacatatc
     4681 ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc cgccggcgcg
     4741 tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata ctaatccgac
     4801 aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc tgattacgcg
     4861 atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat cagaagcatc
     4921 gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga cattttctaa
     4981 atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa cgtcatctgc
     5041 catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta catctggacg
     5101 tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt ctagtacatc
     5161 agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc catccatttc
     5221 tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca taccggcacc
     5281 acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg cgtttttctt
     5341 cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca tttctacgaa
     5401 atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag cttttgctaa
     5461 taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga tacgggcacc
     5521 taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta gttcttgttt
     5581 ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt tgtctgcttc
     5641 tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc cgccgccttg
     5701 ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga agctaagcaa
     5761 tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa ctttgttttc
     5821 ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga ttgttgtgaa
     5881 atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt tagcgacggt
     5941 ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct gcacttcaaa
     6001 ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga tatctgacga
     6061 tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta agattacgag
     6121 gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag ttgccctccc
     6181 taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt gtaccataat
     6241 ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac accgatgtac
     6301 ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa ttcatttggt
     6361 acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg tttgtcaagt
     6421 agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac taaataagca
     6481 agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc ttctacattc
     6541 gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt cccgtagctt
     6601 gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat atctgccatg
     6661 aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg gtaaatttct
     6721 gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga gatcaaaatt
     6781 ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt tgatatttgt
     6841 aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat gaaaaggcaa
     6901 aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca tgctgccttt
     6961 gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg agattctctg
     7021 ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca tcagcaggaa
     7081 tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc cctgcatttt
     7141 caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt tctggaatca
     7201 gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta gagagtacaa
     7261 caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc cattggcttt
     7321 ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa ataattcctg
     7381 tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca gcatctgtca
     7441 tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg acttcctttt
     7501 cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc tgttgcgaaa
     7561 attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga actaccaaat
     7621 gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca aagtaaacaa
     7681 gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc aacggacgaa
     7741 tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta tttaactgtc
     7801 ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga tgagcagtca
     7861 tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa cgaaagttac
     7921 gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca tataaaggga
     7981 gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat tcttctctta
     8041 attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc acttgtaaaa
     8101 gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa attctcgatc
     8161 cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt aatagcggtg
     8221 cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct aaaaaaaaga
     8281 gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta tgacccaaat
     8341 agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc caccgcgctt
     8401 tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca aaaatgaact
     8461 catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa aagatttttt
     8521 ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt taggacggct
     8581 ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc cgatttttcc
     8641 atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta aaacatcatg
     8701 gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg tttttccagc
     8761 acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt gcagttttgc
     8821 tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt tttatattcc
     8881 tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg agctgtctgt
     8941 atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga atttgcttct
     9001 ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt gcagctgatc
     9061 aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt cgttcaattg
     9121 atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa tgaccaaagc
     9181 accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt gttgttgctg
     9241 aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt tattgctttt
     9301 tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata ccaatatcgt
     9361 gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa taatttcgta
     9421 cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt tcacttccaa
     9481 cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac tggaagattt
     9541 cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg ctactgatcg
     9601 tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt attcctcact
     9661 ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt agccattctc
     9721 gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc ccgatcccaa
     9781 ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca aatactatat
     9841 tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg aacatcaact
     9901 tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca attcgaacaa
     9961 aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct gctgttccta
    10021 ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat cctgcgcctt
    10081 ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct tggatgactg
    10141 cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca taatctttaa
    10201 acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca gctaatgcta
    10261 aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga tagatccggt
    10321 tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg gcaatcacta
    10381 aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc ttcgcaccgt
    10441 attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg atcagctgga
    10501 aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga aaacgccgga
    10561 tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac gctcctccat
    10621 gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca aaaacagctc
    10681 cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta aaagcaaacg
    10741 cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa acagcagttg
    10801 gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca ggcatcagca
    10861 aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc atcatccaag
    10921 caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc caaaatacta
    10981 agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt tctgcaacgt
    11041 actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc ccataaattg
    11101 gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg aggggaattc
    11161 ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca aatgctcctt
    11221 gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg ctgacagata
    11281 tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata ctgcttcttc
    11341 catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc ctagactggc
    11401 ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc gttttgttcc
    11461 aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt ctaggagcgc
    11521 tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt cttctggata
    11581 ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt gcgaatcaag
    11641 ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt atgtttcagg
    11701 tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt gactattctt
    11761 tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga aaccgactgc
    11821 atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa tggaaagatc
    11881 ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg cctgaagacg
    11941 tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag catatgcgac
    12001 acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc ccatataatc
    12061 agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt caatgatggt
    12121 tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat tttctaattg
    12181 cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga gttcttggat
    12241 ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata cttgtccacc
    12301 tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt ccataacata
    12361 cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat cccgcacgcc
    12421 taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca aaacatccac
    12481 ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt gttcttcgtc
    12541 aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc ggtgggttcc
    12601 tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt gttgctttct
    12661 tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc tttccagcat
    12721 cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta cttgtttgct
    12781 ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat aaccaacatc
    12841 acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga tctccgctgc
    12901 acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt ctttttggta
    12961 ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat ataatttgat
    13021 tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt tcgtccattc
    13081 acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat atttttggat
    13141 cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca aaatagtcat
    13201 atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt tcccgatacc
    13261 atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt tcaatcgctc
    13321 tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga aaatttcttt
    13381 ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca aagctccttc
    13441 aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta ctaatatgtc
    13501 ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa taaagatcac
    13561 aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg gcatttgtcc
    13621 gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac gaagatttcc
    13681 catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct gcacgatttt
    13741 ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga ttttttgagt
    13801 ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc gctgataatc
    13861 atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag attcttggta
    13921 aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc agcttgaagt
    13981 cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg tcgcagcaag
    14041 ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct ctggtccgta
    14101 gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg agcgttgtgt
    14161 ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta cacgaacagg
    14221 ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga attctcccgg
    14281 ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag gaagattttc
    14341 cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag ttttcttatt
    14401 cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt ttttttcttg
    14461 aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa aggccatttc
    14521 agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa tcacatgttg
    14581 cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa tcatgttttt
    14641 atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg ctaatcctgt
    14701 gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta cttgcggatc
    14761 ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg aatcgattca
    14821 tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca gattgacaca
    14881 tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca tgctggacta
    14941 ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag acattcgtat
    15001 tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc ttttgacgga
    15061 gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat tcctctggat
    15121 aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc atgaatgttt
    15181 gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact tctgcttcga
    15241 acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg accataaagc
    15301 cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg acgatcattt
    15361 tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact gctaattagc
    15421 ggtccagtcc atcgagcttt tttctttttt atacttgtca atgcatagta taacacaaaa
    15481 caaggcgttt cggctaaaag agaatcgtta ataaatgata aataaattaa aaatttcttc
    15541 aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg taagctttta
    15601 catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga catggggctt
    15661 gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga tactaaatga
    15721 ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac ggcgccgtag
    15781 gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta ggtattgtgg
    15841 atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct ttggcattta
    15901 cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct gacttagttg
    15961 ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta gtaaacaaaa
    16021 acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt aacggtatct
    16081 ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa ttctcaggtt
    16141 tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt ttccgtcaag
    16201 cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc ttaggtgaac
    16261 acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg caaatctatg
    16321 aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta ttctttggcg
    16381 tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat ggtatcgcag
    16441 tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt cttcctttat
    16501 cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca cctgctgtta
    16561 tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc gagcaagacc
    16621 gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt gctaaactag
    16681 acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc gtttttttat
    16741 tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt cgtcttttcc
    16801 tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg agttgtcgat
    16861 atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc agttcttggt
    16921 gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac aaagcatttt
    16981 ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga aaaagcaaat
    17041 gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga tggaaaagtc
    17101 tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc ggagctttct
    17161 ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc agcggcagaa
    17221 aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac cgttcagaca
    17281 ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga tgcaacgatc
    17341 gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac gatcgacgtc
    17401 gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac aattgaattg
    17461 actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa agaacaactc
    17521 gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg tgagactttc
    17581 cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa agtcgattta
    17641 gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa cacaagtacg
    17701 aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt tggcacttac
    17761 agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat tctggcaggc
    17821 caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca tccattgatc
    17881 gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta caaagacgga
    17941 aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac acctgctgga
    18001 gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa tgacgatgga
    18061 acaccttacg agagtccggt aaactactgg atgccgattg actggacagg tgttggtatc
    18121 cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg cggttcacac
    18181 ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt cgaaaaagga
    18241 acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc gtacgattgg
    18301 tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc agcagtaaag
    18361 acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa ttgctctttt
    18421 tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc ttgcgccaaa
    18481 gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc agtataaatc
    18541 tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac atcctcaaac
    18601 atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc tttgcggtca
    18661 aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt aaaagctact
    18721 cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa gattcctact
    18781 ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc tacggcactc
    18841 gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc ttttgctttg
    18901 tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc tttagctgcc
    18961 atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc ttgggcattt
    19021 tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc tcccttgttc
    19081 ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc acagccaatc
    19141 tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt cagagcaccg
    19201 atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat catctgatgg
    19261 aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc tgtcagccac
    19321 caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat aaaataggta
    19381 ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc cagatggtcg
    19441 atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc tttaaatggc
    19501 tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta agtttgagaa
    19561 gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat atttccgttt
    19621 ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta gacttttttt
    19681 gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta tacttaattt
    19741 tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga atctagattc
    19801 tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc aattggaaga
    19861 ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg acaatgcaga
    19921 gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta cccaatgcag
    19981 cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa aggtcttatc
    20041 aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat tgtcaaagaa
    20101 atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt cgtttgaaac
    20161 aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat cagaagctga
    20221 agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca gtcattatga
    20281 tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag tcgaattatc
    20341 tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca gagaaacact
    20401 tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg atactttgaa
    20461 tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag attttctaca
    20521 aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt gggatacaat
    20581 ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc ttttcggtaa
    20641 agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt tgaaagaaaa
    20701 aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca aattcagtcc
    20761 aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag cagaagatcg
    20821 tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag aatcaaggat
    20881 ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg aactaggaca
    20941 tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat atgccatgaa
    21001 tgtcgcagaa acagcaagta cattcgcaga actgatcgta gccgatgcta cattaaaagt
    21061 cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa atgctttagc
    21121 tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg aagcacggca
    21181 agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc aaaaagaagg
    21241 ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat tgcatttctt
    21301 tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct ttagtttggg
    21361 catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata tccatttact
    21421 acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga atgtcgatct
    21481 tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata tccaaacgtt
    21541 catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa aaagagttag
    21601 tccagaatgg gctaactctt tttgattaat cattcttaca tgctgacagc aaagtcaggg
    21661 gcaaaccatt gaactgaacc aacttttacg ctttcgcctg gttgaggagc atggatatat
    21721 tgtcctccgc ctaaagcaat cgctacgtgg taagttccgc cttgtgaccc ccagaataac
    21781 aagtctccag cttttgcttg agaaactgaa atttttgttc cagctgattc ttgaggaact
    21841 gtccaaccac cgatgtcacg accagttact tgcatgtaaa catagcgtgt gaaccctgag
    21901 cagtcaaagc cacttggatc tttaccgccc caaacatatg gagtaccaat atatttgtaa
    21961 gcttctgcta cgattgctgc accattcaca cttccagaag gagctggagc aggtgttaca
    22021 ggttttggtt gttcaggtgt tggtgttggc gttggagccg gagtactact tcctgtgccg
    22081 tttcctgtat caacactttg atctgttgat ggtgttgtag gcgcaggtgt tgttgattct
    22141 tctgttgttg aagattcagg cactgttgtt gattcttctg ttgctgaaga ttcaggcact
    22201 gttgttgatt cttctgttgc tgaagattca ggcgctgttg ttgattcttc tgttgctgaa
    22261 gattcaggcg ctgttgattc ttctgttgaa gattcaggtg ttgttgattc ttctgttgct
    22321 gaagattgtg tagctgatga tgattcttca gtcattgaag attgtgtagc tgatgattct
    22381 tctgttgctg agctttgtgc agtagatgaa gcttgagtag cttgtgcttc agcctctgct
    22441 tgttcgcgtg cttctttttc agctttttct tgtgcagctt gttgacgagc ttgttctgct
    22501 agacgttgtt gttcacgaat acgagcttgc tctgcttcag cttctgcttt ttgacggttc
    22561 aagtcagctt tcttgtcttc agcagttgct tgttcagcag ctaatgaagt tttcagtacg
    22621 ttcaaatcag cttgtttaga taaaagatcg cctttttgag attccaaagc agcttggttt
    22681 tcagctaatt cttttagttt agcatcgttt tcagcttttt tgtcttctac agctttttta
    22741 tcttgttttt gttgttccat caagtcgttg tttgctttta ccatcgttgt cattgcttga
    22801 acgcgtccaa tagcatctgc taatgaatca gcattcaata cagcatcgat gtagtttgag
    22861 cttgtgttgc ttacttgtgc ttcgcgtgct tgtttttgga ttgtatcttc acgtttttca
    22921 atacgttctt gtaaatctgc aatgtctttt actaattgtg cagattcttg acgcaatgta
    22981 tcttgttttg ctaataggtc ttgtgcttgt gtgttgattt cagaaacttg gctttctaag
    23041 gcatcgattt gtgattgtgc atctgcttgt tgatttttca agtctgcaat cttttgatct
    23101 tgctgttgta tctgagaatc aaaatcgtcc gccgcggcag cgatcggtga tgctacggca
    23161 gtcaaggtca ttgaacatac cattactgct gatattaaac tctttttcac tcttcattcc
    23221 tccgactggc ttaatttaat aaatatctaa atatatttaa gtctttctta acgatataaa
    23281 aattgtacca tagcagtgtg tcaacgatat aatagttatg ttacaaaaat gtttcaaaac
    23341 aacttttaaa aatgacaaaa ctgttcgttt cagaaaatga tcatgaaaaa gccgcgttaa
    23401 gtctagcatt tcttaagata acaacgattc tttttcgctt tcgacctaaa aataaaaaaa
    23461 gaaattattc atcactgaat aattt
//
LOCUS       DACEOM010000052        22702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEOM010000052 DACEOM010000000
VERSION     DACEOM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.053"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(59..187)
                     /locus_tag="IW781_001906"
     CDS             complement(59..187)
                     /locus_tag="IW781_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1182902.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IW781_001907"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IW781_001907"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1182903.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IW781_001908"
     CDS             complement(1674..2729)
                     /locus_tag="IW781_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1182904.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IW781_001909"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IW781_001909"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1182905.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IW781_001910"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IW781_001910"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1182906.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IW781_001911"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IW781_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1182907.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IW781_001912"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IW781_001912"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1182908.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IW781_001913"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IW781_001913"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1182909.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IW781_001914"
     CDS             10491..12050
                     /locus_tag="IW781_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1182910.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IW781_001915"
     CDS             complement(12161..14092)
                     /locus_tag="IW781_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1182911.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IW781_001916"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IW781_001916"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1182912.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IW781_001917"
     CDS             complement(15021..15773)
                     /locus_tag="IW781_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1182913.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15933..16142)
                     /locus_tag="IW781_001918"
     CDS             complement(15933..16142)
                     /locus_tag="IW781_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182914.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16153..17481)
                     /locus_tag="IW781_001919"
     CDS             complement(16153..17481)
                     /locus_tag="IW781_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182915.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17846..18433)
                     /locus_tag="IW781_001920"
     CDS             complement(17846..18433)
                     /locus_tag="IW781_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1182916.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="IW781_001921"
     CDS             complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="IW781_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1182917.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20013..21302)
                     /locus_tag="IW781_001922"
     CDS             complement(20013..21302)
                     /locus_tag="IW781_001922"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1182918.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21456..22388)
                     /locus_tag="IW781_001923"
     CDS             complement(21456..22388)
                     /locus_tag="IW781_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182919.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
BASE COUNT         6032 a         4874 c         4027 g         7769 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaaataaa aaaccagtca gaaagactgg
    15901 ttttttattt ttttgtctat catcaagccg ttttatcgtt ttttattttc gcttcttgtt
    15961 tttttcatat aaacagaagc aggttttctt tccttatgaa gaagataata ataaagtccc
    16021 atcccgccaa acatgaccaa aaaaccgatg actgatcgaa taggaaaatt aggaacaaaa
    16081 aagagacaga cggataataa tgatgccgga accattattc gataatagag ttgtttatcc
    16141 attttcttca cctcatattt tttgtaaagt aatcaattgt tctaattctc ggtaatctaa
    16201 aaattgcaca cgcagatgct gctcaccttt cttgacatcg aattggatac ttcgccgctt
    16261 tttctttatt cgtttgaagg taacatcaga gagtctatgt ttgatttcct ttaacggtaa
    16321 ttctgacagg gattctgaag gaacatagat tcttaaggat aaaagatact cttgatattt
    16381 ttcatctgtt acctctactt ctagatattc cctaatataa taacaataag cttcgattcc
    16441 attatccaaa gcaacagtcg gatcgataac tttatagcac atcatgcgtg atcgattctc
    16501 gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg cgaacacgcc tttctttata
    16561 atctccttgt tggatatcat aactttttgt tacaaaaaat gaagcattct ttttaggaac
    16621 aggttgcatt tgtgcattct gatactggac agattggatc gtatggtcag tgtaaatgta
    16681 ttcaacgaat ggcggtttca ataaaccgac accattgtcc caagcaagat gatcaaaccc
    16741 aagctggtcc ctaaaatagg attgaaacat ttgattgttt ggcttttgcc agttaaaggg
    16801 gtctcgataa agctggatac atatgacttc gttttctaat tccttcactt tttctacttg
    16861 ttggatgtcg agaaaaatcg gtttagggat gatttgatga taatagcgac tataatacat
    16921 ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga tcgtatcctg gaaattggtt
    16981 gcaatcgact actacttccc cgttttgttg tttcatttta ggtaaatgtt cgatttcatc
    17041 ctttttctca aacaatagcc gttgagagat ctgttttgta ttgtgataga taaactcgct
    17101 ataagctcgc atataggcga acaccccatg agcaatcatt tttttctgaa tgaagtctcg
    17161 aatcaaaaat tgcttttctt tgaaaacaga gatagacaat acatttttta tcaataattg
    17221 attatcttcc aagcgaaaac tacattcgtt ctcgtcattt cctacaacta accaactttc
    17281 gtctcttacc gatgctagca ttattttttc ttgaagcttt cgaagatcat acggatccgc
    17341 ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac agacgtgtca aaatagccca
    17401 atcgcttatt tcctgttttt tttcttcttc atttaagtct gtgagctgga tagtcatcaa
    17461 taatttatct tcgccaatca ttttctcacc tctttcaaat ctttttataa tttaattata
    17521 accaatgata aagaaaaact aaaactttat gtcttagaaa ccaatttgga cgattacatg
    17581 atagactaag taaaaagaaa caaagaggtg tttacaatgg ggttgaaatt tgaacgtaca
    17641 gataatttag ataaactttt cgaatcattt gctgtcgatc cggaaaaaac agttgcacca
    17701 aaagaagaga tcgatcgatt tctaaaacca cagccacaaa atgaaaagaa aaaagacaag
    17761 aaacaaaagt aagttaagct caaaaaaaga cttaccagcc aaacttttat gaaagagttt
    17821 gactggtaag tcttttttat tctttttatg attcaattgc ttgttcgatt gctgcccacg
    17881 cttcttcttt tccctgtttt gtaacggaag aaaagagaat aaacgtgtca ttgcgatcaa
    17941 aatccaacgc ttttttgatt tcgctctcat gcttgttcca tttaccacga gggatcttat
    18001 ctgccttagt ggcaacaaca attacaggga tatcataata cttcaaaaat tgatacatct
    18061 ggatatcttc ttgagaaggc ttatgacgca aatctactaa agaaacgacc gcacgtagct
    18121 ggtctctagt cgttaaatat gtctcgatca tctttcccca ttttgctcgc tcagttttgg
    18181 atacttttgc atacccatat cccggaacat cgacaaaatg taatgcgttc tcgatcaaat
    18241 aaaaattcaa tgtctgggtc tttcctggct taccggaagt acgggctaaa ttctttcgat
    18301 caatcaatgt gttgataaaa gaagatttcc ctacatttga tcgtcctgcc agagctattt
    18361 ctggcaattg tgtatctgga tattgttttg gtgccactgc acttatgata atttcagctt
    18421 gatggacttt catggtctct ctccttaaca aggtgatgaa taaagcaagc ggctgtcgca
    18481 aaagccaaaa tgacttttac ggcagcctct tttcatcaac ccgctttttt ctctgaatct
    18541 ttatagatta ctgttggctc accagacaat tcagctgctt cttttgtgat aatcactttt
    18601 tcgatgtttt catttgaagg gatatcaaac atcacatcca tcatgatttc ttcaatgatg
    18661 gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt ttttcgcaat ggctctcaac
    18721 gcttcaggtt cgaattcaag ttcggtatta tctagagaaa gaagtttttg atattgtttg
    18781 actagcgcat ttttcggctc agtcaagatc cgtaccagat catctgttgt caatttctct
    18841 aaagctgcca taacaggtaa tcgtccgata aactcaggga tcaatccgaa ttttagtaaa
    18901 tcttcaggaa tgatatgctg catgacactt tcgtcttcag aaagtttttg gttttgtgta
    18961 ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa tcgtttcgat gccatcaaat
    19021 gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct gaataaattc ttgatgtgga
    19081 tgtttacgtc cgccctgagg tggcacactt gcaacagttc cttctagaat tttcagcaag
    19141 gcttgttgga caccttcgcc ggaaacgtca cgagtaatag atacattttc gcttttacgc
    19201 gcaattttat cgatctcatc aatatagatg atcccttttt ctgcaagttc cacattgtaa
    19261 tcagcagatt gaagcaattt aagcaaaatg ttttctacat cttcccccac ataaccagct
    19321 tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat tcaatgtttt tgctaatgtt
    19381 tgtgctaaaa atgttttacc agaccctgta ggtccgatca agcagatatt gcttttttgc
    19441 agttcaacat catctgtgtt ttcttgtgct tgctgattga cacgtttata gtgattgtat
    19501 acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa tcacgtagtt attcaatact
    19561 tcaagaattt ctagtggttt agggacttca gtgaattcgc gtacagcttc ttcataaaat
    19621 tcttcatcaa tgatttcttt acataggtcg atacattcat tacagatgta gacccctgga
    19681 ccagctacga tctttttcac ttcttcttgg gttttcccgc aaaaagaaca gcgtacggtc
    19741 tcattgctac ttggattgtc atacatggtc ttcacccctt aacttgcaaa aagtatgcaa
    19801 gaaactatca aagactttac agtcttttac tatcataaca tagtttagta aaaagaaaaa
    19861 gtgtttaaca aaggatcaac tgacctatac ctttgcattt tttactctta acaaaaaata
    19921 aggaactgtg acaaaatcgt gtcacagtcc cttatttcga ataaacaatt ttctagaagt
    19981 agcttcttca gaaatgattt attacttgat ttctattctt tcgcttctgt cgctgtacct
    20041 gtgatcaatt cgattgcttt tttcattgtc acatcatgtt ccagcatatc ttctgtcaac
    20101 acgcgtttga tttgttcgat tggcatgttg tatgtttctg ataattcttg gatttctgca
    20161 ttgatttctt cttctgttgc ttgaagattt tcagcagctg caattgcttc gatcacaaga
    20221 tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt tatgcaagtc ttcttctgta
    20281 gaaccagtta attgatagta catttcagga gagatacctt gacgttgcat gttgttcaag
    20341 aattcgtcca ttgaacgatg tacttcatca tgaaccatca cgtgtggcaa ttcaacgatt
    20401 tctgcgtttt caacagccat acggatcgct gcttcatctt ttgcatcgtc cgctgctttt
    20461 tcttttgttt ctactaattc tttgcgatat ttttctttca attcgtctaa tgttgatact
    20521 tcgtcatcaa catcttttgc gaattcgtca tctaattcag gcacttcttt tgcttttact
    20581 tcatggattg tcactttgaa gactgcttct tttccagcaa gatcttctgc ttgataatct
    20641 tctgggaaag ttacatttac atctagactt tcacctgatt tatgaccaac taattgatct
    20701 tcaaaacctg gaatgaatga gcctgaacct aattcaagtg agtaattttc gccttttccg
    20761 ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga ttactactgt atcgccattt
    20821 tcagctgcag catcttcttt gataactaat tcagcttgtg cttcttgttc acgtttgata
    20881 cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt tttcaacaac tagatcttta
    20941 tattgtccta attttacttc tggtttcact gttacttcag cagtgattac ccagtcttga
    21001 cctttttcca tgctttcaac atcgattttt ggttgagaaa caggatcgat gccagcttct
    21061 ttgacagcag cttcataagc ttctggtaat acagcattca atgcatcttc atataaagct
    21121 tcttcaccgt acatacggtt gaatacttga cgagaaactt tccctttacg gaatccaggt
    21181 acgttcaagc tttttttcac tttattaaac gcagttgtta atcctttttg gatttcgtct
    21241 tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt ttttttcaaa tgttgcagtc
    21301 atttaattcc ctccgagttt agaaattcat tttatacatc aaaaacgttt catgcatacc
    21361 tttaaatagt acactaaccc tttcaaattg taaacatatt gaatggatag aatccttgtt
    21421 tttcataaca tttttatctt tttcactgat aaaatctaat ggaagaagat catttgctgc
    21481 aaaatttttt ctcttttttt taaaaaatct tgtttttcat cttctgtacc ttcatacatc
    21541 gtttttcctg tacacgcctc tacagtcagt tgaaaccagc gcttcgggtc ttcgatgtat
    21601 gtatcataga caggataaac aattgcagat tgcaacgtga attcttcttt taacacggaa
    21661 ctaagcagcg catcttcatt cccgtaacga tctgaaaata cacataattg ctggtaactt
    21721 gcctgctgct gcggctttgg cagatctttt ggtatgactt tgactaactt ttcttcaatc
    21781 gtcaaataga aaacaggatg gcattcgttc aattgcctaa gtgtctccag taattttgcc
    21841 cttgctaaaa tatggacctc tggcgataca ataagtttac tcgctgtttg gataaactca
    21901 tgataaggaa gctgttcgat ctcatcaata gacgcaagct gacgtgcagg ttccaacgaa
    21961 ggaagttcct ccgatttatt gtggagtgcc ttgatcttct gcctctccat ttgtccggat
    22021 aattcttctg caaattggat tttttcaatg acccgctgct cgaaagcttc ataaaaatct
    22081 gtacttgcta tcaattttct agcatataaa aaatcttgtg tactgattaa caagtcaaaa
    22141 taaaattcag caaactcctc attttgaaga tactgttttt catgcaagag tgcttgcttc
    22201 aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa caagcaggct attagcctca
    22261 aagctttctt tcaactcgta agctcgttga aataattctt ttgcctgctg atattgctgg
    22321 tttgctagtg cttgttgtgc cactcgcatt aaattctcat atttttctgg aaaatcaatc
    22381 ggcttcacgc agtgtcctcc tcttgctcat attcagctca tgttcaatag tagcaaaaca
    22441 aaaaaagcat gacaattaaa aataactgaa gcaccctcta aaagctagat tttagaggct
    22501 ctatactttt tgatgttggt ggatgacacc agcatctttt ttattttcca aaacatatag
    22561 aaaagacacc ttgaaatcct ttagaattaa tttggcgaac caacccaaaa ggaggaatca
    22621 agatgtctta cactcacctt atcaaagaaa cattagatat tctagactta tcagttactt
    22681 ttaacgaaaa ttgtctcaca aa
//
LOCUS       DACEOM010000053        22205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEOM010000053 DACEOM010000000
VERSION     DACEOM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.054"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..549
                     /locus_tag="IW781_001924"
     CDS             <1..549
                     /locus_tag="IW781_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1182920.1"
                     /translation="MLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDP
                     NGRTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNA
                     MARKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEV
                     ISEENEDVQTGLFDASNPPLNK"
     gene            598..1491
                     /locus_tag="IW781_001925"
     CDS             598..1491
                     /locus_tag="IW781_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1182921.1"
                     /translation="MDYIGQLNAFDNWLEYNELGAGPQLLWYKLMAIANKSGWQSELS
                     IANTRLQAMTKTSEKTLINNRNQLIQNGLLQYKKRGRTKAGVYILSDLTGNFTVKTTV
                     DNTVENSATENIPVDSKVNPKVNREVNPSVDSTVNPSAYINNTKQNKTNKEDDDIGVY
                     EFIQKNWGKAPTGLLQGALGPMIKAWGADMILFAFKLAFENNVEMPGLKKYVEAILNS
                     WSNQGIKTMESAEKAQEAFKNKKKQNYLPKRQNNVRREKLPDWVNKPQEEKTLDPDKK
                     AELEARFAAYQAKKEALLENE"
     gene            1484..1843
                     /locus_tag="IW781_001926"
     CDS             1484..1843
                     /locus_tag="IW781_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1182922.1"
                     /translation="MNKYRNRKTIHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVNVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            1858..2019
                     /locus_tag="IW781_001927"
     CDS             1858..2019
                     /locus_tag="IW781_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1182923.1"
                     /translation="MTTEEVIQMRIRSIQREIDELERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            2016..2321
                     /locus_tag="IW781_001928"
     CDS             2016..2321
                     /locus_tag="IW781_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182924.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEGKEIVAEVQKLGKSMWNEKPK
                     RRTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKERQFRYVEEK"
     gene            2321..2632
                     /locus_tag="IW781_001929"
     CDS             2321..2632
                     /locus_tag="IW781_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182925.1"
                     /translation="MNELITKVEKWAKDKGLDHADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGQMVDGVFVKSSDLEDS
                     K"
     gene            2629..2874
                     /locus_tag="IW781_001930"
     CDS             2629..2874
                     /locus_tag="IW781_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182926.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2871..3260
                     /locus_tag="IW781_001931"
     CDS             2871..3260
                     /locus_tag="IW781_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182927.1"
                     /translation="MIPRFRAWYTPFKGETFGQEMKYGQTGRLITHAEMAPDKYVLMQ
                     STGMKDKNGVEIFEGDVVSVSVRNGFDYLDNKVCIVKNSIGHSGLVCATVDEDLEYRI
                     FNTELFEEYTYEVIGNIYENSELLEEK"
     gene            3260..3559
                     /locus_tag="IW781_001932"
     CDS             3260..3559
                     /locus_tag="IW781_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182928.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMEIIKTEL"
     gene            3581..3808
                     /locus_tag="IW781_001933"
     CDS             3581..3808
                     /locus_tag="IW781_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1182929.1"
                     /translation="MNDKFINKNDLDSLLVGYVPKRYLTQKEAVHYTGTSAGTINEWV
                     KKGLKVIIFGENSRPKYDIKDIDEFIAKYKV"
     gene            3955..4134
                     /locus_tag="IW781_001934"
     CDS             3955..4134
                     /locus_tag="IW781_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182930.1"
                     /translation="MELKVIGLSDIEKMQGEHCLIIISNGQMKSVELPSFGTIVIESH
                     CNKVKQVKEEVKQLF"
     gene            4237..4824
                     /locus_tag="IW781_001935"
     CDS             4237..4824
                     /locus_tag="IW781_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="positive control factor"
                     /protein_id="HAQ1182931.1"
                     /translation="MNDLIQEYKNDLKMLKKQHQKILNKRYRTPVKEHGRVIHKLIDD
                     RSPQDIADQKVIAEAISTTEYALFWLETGREKPFDDEQAKKIPKHRRAVKVADIDVMS
                     YQVYLQEIEKPAEETISPKKREMLLQVTEIESLLSNKELTLFHLINKDLCTYGEAAEK
                     MDLAVGTVKSMSQRIKNKIDNYFEYGHQINLFEIS"
     gene            5078..5302
                     /locus_tag="IW781_001936"
     CDS             5078..5302
                     /locus_tag="IW781_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182932.1"
                     /translation="MRNYWYVSLINDYPRCKDCRDKRVVQSVQMKSKYSVIEMIREAE
                     PNEIDACRLVYCGHGFYSDKHIQENLSKYI"
     gene            5333..5950
                     /locus_tag="IW781_001937"
     CDS             5333..5950
                     /locus_tag="IW781_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAQ1182933.1"
                     /translation="MVELKNNKQEQFAKEYLIDLNATQAAIRAGYSSKTAYSQGQRLL
                     KNVEIQTRISELMNERSRRTEITADRVIQEIAKIAFSDIGEYMTWDQDGNITMLSSDN
                     LDTSVIQAIKSNRTLRPIGDGEEVIDSSLEVKFHPKLKALELLYNHVNGSDKLSKAQV
                     RKAIAEAKIIENKADKLTAGGKVNNLLQALLDVKSGGDGSNGTEV"
     gene            5934..7223
                     /locus_tag="IW781_001938"
     CDS             5934..7223
                     /locus_tag="IW781_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAQ1182934.1"
                     /translation="MELKFSPKQISNINQVVQGITFELNEGTPRSGKTTSDIFKMADF
                     YLRSPDQNHLVTAYNQEQAYRMFMDGDGLGLVHIFDGCSDIRHDEHGDHLLLYAPNGE
                     KKIYYKGGGKINSVGAITGMSLGTVTFLEFNLLHKDFINECFRRTFAAEWRYHLGEQN
                     PPAPNHPNLELLERFEKSGRFLFRHWTPNDNPILSEERKKELFDELSSSEYLLERDWY
                     GHRVLPQGVIYAMFGKNNKATEIKGNIIETFFTADGGQADATTCAFWVVTHHEGKFYL
                     YRLANYYHSGTETGETKAMSIYAKEIKKFVEWCYTKWKHLPRWNWFFVDPACKTLRTE
                     LDLIGIETDKADNNSSDKVSSNGLKIEVGIERLQNTMTSGQFIILENGEEYDHYNFEK
                     EISMYVRNDNGLPIDKYNHALDEARYGNNYFYKTYIA"
     gene            7239..8738
                     /locus_tag="IW781_001939"
     CDS             7239..8738
                     /locus_tag="IW781_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1182935.1"
                     /translation="MFDKLKALFRIGGAKIGMVETLNSITDHPKIAMSDSELSRIRNN
                     KEIYRNVYGDIEYINSDGYRQTRPFHSLNVSKVVSRKLSKLVFNDGCNISLDDEKADE
                     FLQSVFADNKFRKNFGEELEAGYAIGGLALRPYVDTNSGKIKISFCRADTFFPLQSNT
                     NDISEAAIATVTQQAEGQKTIYYTLLEFHEWVDGKYRIRNELYRSEEQKQVGVRIPLN
                     SLEKYKNLQEETILDGFSRPLFVYIKLAGKNNINLDSPLSLGVIDNAKRQLADINEKY
                     DEFMWEIEEARRKILASDHFFRVKYDSNGKPVKRFDSKTSVFQRLKSDEPFIDEFAPS
                     LRSTEFIASINFILRIIELQTGFSSGTFSFDGQSVKTATEIISENSETFSTRSDNVLI
                     VEEALKELITTIFELAEAYKLFNPVKELGINIDFDDGVFQSQDAKADYYSKLVTAGLT
                     SKLNAIQKLTGVTEKEAKRVVYEIRAETLDMDYSEHEQNIIEGQLGSEE"
     gene            8738..9901
                     /locus_tag="IW781_001940"
     CDS             8738..9901
                     /locus_tag="IW781_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAQ1182936.1"
                     /translation="MVSPHQLDLWSSNMAHLYQSLEGELIRIIIKRLNSGHDNILDWQ
                     REKLQQLHLFNKETAKVISQITGIAESEIESMFDSSGEKIIRDLDKQLPYDPKPLPSN
                     LDNVMKAYHDQVWSDINNYVNQTLLSTNFGYGTATTQMYTEIINKTTAAFNSGLFTFD
                     EALERTIQGWAQKGIKSTFIDKGGHTWSLDRYVRAVLKSTLSNTYDALRKDRMSEYSV
                     HTVLVTSHMGARQACSKIQGHVVDLRPVSELPLNWKYRSIYDPYWQAEYGTAGGHRGV
                     ECQHLHIPFIPGVNTNNQPKFDAKENKKVAELTKKQRYLERQIVKYKKNRMVSEALEQ
                     DENAKEWAKKIRVTQSRLRTLVDSDEYLSRNYAREKVYTPINTLLKDFHYDDF"
     gene            10012..10587
                     /locus_tag="IW781_001941"
     CDS             10012..10587
                     /locus_tag="IW781_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAQ1182937.1"
                     /translation="MKKEDLIALGIDEEIAKSVMALHGKTVTQLNAQVATAEQERDQF
                     KEQLDSNQTELDALKEAAKGNEGLTQQLADLQSKFDAAKSESETKLAEQQKDFAIKLA
                     LKEANALDENIVLGLLDRDTIKVTDEGLQGLDEQLKSLQENKKFLFNAIDNSNPTPQI
                     VMPGNPKNDQKTSGPSLVETQAELNKFRITQ"
     gene            10606..11556
                     /locus_tag="IW781_001942"
     CDS             10606..11556
                     /locus_tag="IW781_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1182938.1"
                     /translation="MKKNNLFKMNLQYFANAETTFNPDDVTMQTAKTGSIPINQSTEI
                     ITEVKNGSAAMRLAKAVPMAKPVEEFTFMTGVGAYWVGEGERINTSKPTFAKATMTSK
                     KMAVIIPTTKENLNYSVTNFFELMKPEIAEAFYKKFDQAVFGGVENPYQFSVLKAATD
                     AGNVITETANKYDDFNDAIAAIEENDLEPNGIASTRAQRRKYRATKDGNGLPIFNTAN
                     SNGVDDILGLPIAYTPKGSFGTGDTAPVELVGDWNYAYYGILKGIEYEILTEATLTTV
                     VDGNGNPINLAERDMAAIKATFELGFMTVKDEAFAVVKKA"
     gene            11574..11822
                     /locus_tag="IW781_001943"
                     /pseudo
     CDS             11574..11822
                     /locus_tag="IW781_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330991.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
     gene            11855..12259
                     /locus_tag="IW781_001944"
     CDS             11855..12259
                     /locus_tag="IW781_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182939.1"
                     /translation="MPYIEFEEFKDLTGKTDDFKAAFENHLSKATAILDSITNYFYQF
                     NKIEEDPIGFRVKQFKLALCSQIIYFDEVGADTYESINNTPQSFSVGRTSISNSSRYN
                     PSGKNESKSLVAEDIYIYLEGTGLLYRGVPSW"
     gene            12253..12594
                     /locus_tag="IW781_001945"
     CDS             12253..12594
                     /locus_tag="IW781_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HAQ1182940.1"
                     /translation="MVMPKPPVQFLVDSFIYREYLGEGEYNQPIYGDYVTIENCRIDR
                     GSQYSFSPSGKQLLYNAVIFCYKTLTTPLPNFKEQSLVIYDGKEHVITKIEKITEAYS
                     DAIYSYELEVI"
     gene            12594..12920
                     /locus_tag="IW781_001946"
     CDS             12594..12920
                     /locus_tag="IW781_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAQ1182941.1"
                     /translation="MSIKVNLDGVRAKVSPQAMKRGRYALANQAMADMNSFVPKKNNI
                     LRQSAHIKSDGSAILYETKYARRQFYLNGKKYTTPGTGPRWDLKAKSLYMPSWKKAYL
                     KGAGIQ"
     gene            12920..13321
                     /locus_tag="IW781_001947"
     CDS             12920..13321
                     /locus_tag="IW781_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAQ1182942.1"
                     /translation="MDFIDRIKDKINSIPELPLKMKKGYLSADESLVIYPLPGGQNLV
                     EYYDGIKDVQLNFEIAMKSKDGHRIEQTLWLISDSLERVSDVASSDGSFEFNNLTITS
                     KPFINDADEQGWFVFLLDFQTKLTTFEGENK"
     gene            13318..13776
                     /locus_tag="IW781_001948"
     CDS             13318..13776
                     /locus_tag="IW781_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1182943.1"
                     /translation="MRRKNALQSYFIQLITTTNADTPSEDGWLELAKWISNVDDNSNE
                     ESETTGYYDGNGEGETDVTSHQLGYSFTGLYDEDDAAMAAIEAMIGKSGDARKVWFKV
                     VSASGKKQRIGKATVTEPVAQVGDATAYGDFSCGIAFDSTPEAKDVPVAP"
     gene            13766..14113
                     /locus_tag="IW781_001949"
     CDS             13766..14113
                     /locus_tag="IW781_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HAQ1182944.1"
                     /translation="MHPNAPQNVTGVLNDGSISLSWDAVPKAQAYVIHYSNANQSDPH
                     DATMMGYSEKTSWTLAAEDVPTLEPGNKIYLYVQAYNVLGKGKDEIEKARYLHDGPFI
                     GSAWSKSVVLIKK"
     gene            14154..14603
                     /locus_tag="IW781_001950"
     CDS             14154..14603
                     /locus_tag="IW781_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182945.1"
                     /translation="MTNKLSFQIEKKGFPINIGEVEFFFGTTPEELTRFFDTQAEFEE
                     QVKELKQQLKQIKNIEQPEKEDAIKIIDLTKSLAKAEYDSLLGKGSFEKIYSVYRDVE
                     QLIDLFDPISFEVAEAIEKEALKRKDTLSKKKADLLKKKALKNKKKK"
     gene            14612..15193
                     /locus_tag="IW781_001951"
     CDS             14612..15193
                     /locus_tag="IW781_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182946.1"
                     /translation="MRLNDPLVTSIEFDGIELPIDLTFDNVLDVFDILEDSDLFPEEK
                     VNMCLELLISDFEKFFQGSSEQQFLLFNHILENYISVGDSDGVETDRLGNPMPNAVKE
                     KKTISLVHDAKYIYASFRQIGINLFEEQGRMMWEEFQALLESLPDDTILARIIQIRTW
                     EPSKGESAKEKERMRKLQQKYALPDSEVGEDDG"
     gene            15186..18668
                     /locus_tag="IW781_001952"
     CDS             15186..18668
                     /locus_tag="IW781_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1182947.1"
                     /translation="MADGRVEIEVDVDGKGVTILNKGLDQLEGKSNKAGASIKNLVVS
                     LGLVKVAAAAFNVLKNSLDSAISRFDTMQKFPKVMKALGFSAEDSQRSINKLSDGIDG
                     LPTKLDDVVASTQQMTAITGDLDRSTDTVLALNNAFLASGASTEDASRGMQQYNQMLS
                     TGQVDLESWKTLQETMPLALQKTAEAMGFVGKSAQRDLYNALKEGTVTFDQFQDKLIE
                     LGTGTGMLATLAKENSLGIATSFGNLSNAVSKGVANLITKFDELVQKLTGKTIAQNID
                     SMKSIINKSFEEMSKVMDILIDNTDDMISAFKGLLDIVELLAPAFIAATGAYVGFKTA
                     LALGTLISFVGKIYGVITALGSMVSMFGVSGTAYALLSAIIPAGLTVFQLLSGVIGAA
                     VAAFIYFYKTSETFRNGINKTIEVVKSGLIKSFEYLKGVFISILPTLQKVADTVGNYL
                     VKGFQKVVEVGSAIASVAVPAFFNFVDAVKRIVSSGIERFGSTLSQIGSVLSGIFSSG
                     IELAGNLLEKFGGALGKVGGAVSLVIGILTKVAIAALGLTGPFGLAVSLIISFISAWA
                     KTGDFSADGITKVFDQLSETISNVADSISQYLPQIIESITSVITSIVDKIVEMLPQLT
                     EIAIQLIQTLTDAIVTYLPKLIEIATKIITTIVQGISLALPALLLAATEIITKLISAF
                     AELLPKIIEVGTNLLTMLIQGIVAALPTIIEVVIQIINTLIDGFLTVLPMLLEVGLQI
                     ITSLVNAIITALPQLIEASTVIVTTLLTTIIEALPTLISAGIQMLMALIGGIISILPL
                     LINAAIQITMALISALISALPQIIAAGIQLLLALIQGIISILPQLVAAAIQITIALVN
                     ALISALPQLISAGIKLIVALVDGVISVLPQLVSAAIQLMAALFKALVGAIPQLLSAGV
                     QLINALIRGILSLLGQLLSAGARLITGLLSTIAQFLGQMVNAGANLIRNLVSGILSVI
                     GSVTSAVSNIGNSIIDTLSGIDLFEIGSNIIQGLINGIGSMVGAVASKISEVAGNIKD
                     KITGALGIHSPSRWMRDYVGKFIPQGIAVGIEADAKSAYSAMNKLSNGLMNSITPESA
                     LGTSRMGMASVGSQIVNNTYNNQKQFDVEKLAQVIAKQPVRVSSYLDGTLVGDDMDQR
                     FGKVLNRRSYMRGG"
     gene            18673..19506
                     /locus_tag="IW781_001953"
     CDS             18673..19506
                     /locus_tag="IW781_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HAQ1182948.1"
                     /translation="MNEKTRVYLAFSDEIVELTNNSYLRLIDINIGMPVAKNEFVEFS
                     GTNGKRLSNSSFDAFPITLSFDIRSREQSMFDLVLQKTELRELFTREPEFYLIYSKEP
                     GKKYRVVYDSIDDERKGTIYTRYTVNLGAIRGYSESIATTLTDFNLEEEWQFSQGLVA
                     EDYKYTHQTSHFIIYNAGSFEIDPREHYLRIVLEGESEGNVTIFNKTTGDRFIYYPSL
                     STNLGQTLVLDGVIPKLNGVSCGINTNHGLINLVAGVNEIEIQNITRVKSSWDFRFLY
                     K"
     gene            19515..20558
                     /locus_tag="IW781_001954"
     CDS             19515..20558
                     /locus_tag="IW781_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182949.1"
                     /translation="MTDLIIRDYEQTKEEILVGYGKDSFYENWQQNETWEIGFTVTNN
                     WTNQEVFDLVEYESSVFYNGQEFVIKEMTRKAVGQLLTKQVVATHIYYTVQDGYQYNK
                     VTGTRSISQLLTHVFSAGSRGFTWEVIDPNKKFLTVEQENFGNANYLKLINEILSDYN
                     AVVIPNNKHLTFYPASEYGQKTEEQIRYKYNTDEVSFDIDTYSLKTQIKGYGKLKEGA
                     NTENPKDSDYVFTPITYTSPESEKWGIRIQDPVSDERYTVSGNMLERLKADLQDYPSI
                     SGSVTLKWKISPSKGDHVPFIYEPLNINTYIQVVGIKTYPAIPNKPPEIILSNNKKTM
                     TSILAEMAQKGVV"
     gene            20558..21589
                     /locus_tag="IW781_001955"
     CDS             20558..21589
                     /locus_tag="IW781_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182950.1"
                     /translation="MGLLKLFSNRISKEWKEKFNKNIDYLSNLEKKLSDQDKSTNSRI
                     DNLVLHSGGESPNEVVDARVNNKGEVFDTLHGRLLEHENLSDEQISELNTNMDSQKEQ
                     VQQLNKSVQQIIGGYSEPINMYVSKNGSDISGDGSEEKPFLTIQTAVNNVPLITTSQI
                     TIWVDSGAYLEDVMIRNLNFTSFLIRPIDNFDTIDPLKSDLPVKVRSICFTACKGYCQ
                     VAGMQIVDTANGADYGIRNEQSGYMAINRCKFAENTKTLARYNAVYVGGTSKINMYGD
                     TTLINQKVAIYAVLMGEIFVSAFGSGNDVGILCENGTVRGTVSTSFATTPTKISGYGL
                     VITKGTVLQ"
     gene            21589..>22205
                     /locus_tag="IW781_001956"
     CDS             21589..>22205
                     /locus_tag="IW781_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1182951.1"
                     /translation="MVYKTNESIIVIQAEATSPNRTNVVFWSHDRGTAKLRMKLVRKN
                     GIPQSLPEGTTVPIRLMFKSATAEGGYGKHDYLATVEDPVNGIVSIVLEDNILGYVGT
                     VEGSVYIDFPNDRSLDTAGRFTFYIKRSPIDDSTPELEDYYFNGFSQTIDKIEKILAD
                     GKQEIEQKITESETQIDAKLKDTNDKITKANQDVATINTNIDKAN"
BASE COUNT         7779 a         3507 c         4661 g         6258 t
ORIGIN      
        1 atgcttggct ataaaggata tatccaatta gcccaacgat caggtaaata caaagcatta
       61 aatgtgattg aagtttacga aggagaacta aaaagctgga accgactgac agaagagttt
      121 gagtttgatc caaatggtag aacatctgat gaagtcattg gatatgttgg ctattttgaa
      181 ttactgaatg gattcaagaa aactgtctat tggaccaaac aagaaattga agctcatcga
      241 attgctaaca ataaagatcg agataagaca aagttaagtg gtgtgtgggc atctgattac
      301 aatgcaatgg cacgaaaaac tgttttgaga aatcttcttt ctaaatgggg gatcttatcc
      361 attgaaatgc aagaagctac cacatcggat gagagagtcc aaagagttca agaagatggc
      421 agcattattg ctgaaacaga agttgaggaa gatattcctg aaagaaaaga agcagaggtt
      481 atttctgaag aaaacgaaga tgtacaaact ggattatttg atgcatctaa tccgccgtta
      541 aacaaataat gagggagttt tctccctcaa attactagaa cgaaaggagg aacacaattg
      601 gattacatcg gacagcttaa tgcttttgac aattggcttg aatataacga gcttggcgct
      661 ggtccccaac tgctttggta taagctaatg gctatagcaa acaaaagtgg atggcagagc
      721 gaattatcga ttgccaatac aaggctacaa gcaatgacta aaacgtctga aaaaacattg
      781 attaacaatc gtaatcaatt gatccaaaac ggactccttc aatataaaaa gagaggtcgt
      841 acaaaagctg gagtttatat tctttctgat ctaactggaa attttacagt aaaaactaca
      901 gtagataata cggtagaaaa ctccgctact gaaaatattc cagtagatag taaagtaaat
      961 ccgaaagtaa atagggaagt aaatccttca gtagattcta cagtaaatcc ttcagcttat
     1021 ataaacaata caaaacaaaa caagacaaat aaagaagatg atgatatagg cgtgtatgag
     1081 ttcatccaaa aaaactgggg gaaagcacct actggacttt tgcaaggagc attaggaccg
     1141 atgattaaag cttggggagc agatatgatt ctctttgctt ttaaattagc tttcgaaaac
     1201 aacgtggaga tgccaggatt gaaaaaatac gttgaagcga tattaaattc atggagtaat
     1261 caaggaatta agacaatgga atcagcagaa aaagctcaag aagcttttaa gaacaagaaa
     1321 aaacaaaact atcttcctaa acgtcaaaac aatgtacggc gtgaaaagtt acctgattgg
     1381 gtcaacaaac ctcaagaaga aaagacgcta gatcctgata aaaaagcaga attagaagcc
     1441 cgctttgctg cttatcaggc taagaaggag gcgcttcttg agaatgaata aatatcgtaa
     1501 tcgaaaaact atccatcgag gtatcaagtt cgattctatc gcagaagcag agtactacga
     1561 tctagcctta tggcaagctg aagcgaacgg ctggaaagta aaacttcagg aacgatttga
     1621 gctgatgccg aaatttgaac tagacggaaa gaagtatcgc aagatcgagt atattcccga
     1681 cttcacattt tataaaaacg gcaaacttgt caaagtcgta aatgttaaag ggatgcagac
     1741 aaaagacttt aagatcaagg cgaaattgtt ctgtcatcaa tatcaagtgc cgttgatatt
     1801 agctaaaaaa tatcggaata cgttcaagga agagcgtttt taacgaggtg gtccatcatg
     1861 acaacagaag aagtgattca aatgcgtatt cgaagcattc aacgtgaaat tgacgaactg
     1921 gagcggacaa aggcagtgat ggtcaatgaa acggcgagaa aggcaatcga tttacacata
     1981 gagaacttaa gaagggaaat tcgtagattg gaggaatgag cgtggataag aaagcagcaa
     2041 tgaaacgaat catcgaactg acacattctg agaattggca agaaggcaaa gaaatagttg
     2101 cagaagtcca aaagctcggt aaatcaatgt ggaatgaaaa gcccaaacgg agaacgccga
     2161 gaaaaattgc aatctggcat ggtgaccgaa ttctagtgac aggtactgct gaacagttat
     2221 ctgaaattac tggattaagc aaaaacatta tctgggatag agctaggagc ttatggattg
     2281 attcaaaaga acgacagttt aggtatgtgg aggagaaata atgaacgaac taatcacaaa
     2341 agtagagaag tgggcaaaag ataagggatt ggatcatgcg gatccaaaag cacagttttt
     2401 gaaagtagct gaggaattcg gggaaatcgc ttcagcaatg gcaagaagta atgatgagct
     2461 atttaaagat agcgtaggag acgtaatcgt cactctgatt atcctttcca tgcaaaaagg
     2521 gacaaacgta caagagtgtt tagaaatggc gtacaacgaa atcaaaggac gaacaggcca
     2581 aatggtagat ggtgtattcg tgaagtcgag tgatttggag gatagcaaat gataaaaaag
     2641 ctcattcaat tcagcatgga tttatatgat atcgaatcag gagcaacagt atctgtggaa
     2701 tcggatcatc taattataaa ttttggtgga aagcgccaga ttattttgtg ggtagttgat
     2761 gatgtactgt ttccagaaat cgtacatgat ttcgaagaat caaaagctgt tgagtttgaa
     2821 atagtgaaaa aagtaatgga attgattgaa aaatacgagg aggacagcga atgataccga
     2881 ggtttcgagc gtggtacaca ccatttaaag gtgaaacatt tggacaagaa atgaaatatg
     2941 ggcaaacagg aaggttgatc actcatgctg aaatggctcc agataaatat gtgcttatgc
     3001 aatccacagg aatgaaagat aagaatggtg tggagatatt tgaaggggat gtagtatcag
     3061 tcagcgtgcg aaatggattc gattacttag ataataaagt ttgtattgtc aaaaattcaa
     3121 taggacattc gggattagtt tgtgccactg ttgatgaaga tttagagtat cgaattttta
     3181 acacagagct gtttgaagaa tacacgtatg aagtcatcgg aaatatatac gagaatagcg
     3241 agttattgga ggaaaaataa tgaaactaaa agacggattt tacgctagta gtcatggtat
     3301 cggcggttta atgctagata tgccgacaaa gaatcctaaa aaacgtaaga aaccaaaatt
     3361 caaagtcggt gacatggttc gctgcgaagc agaaggattc atctatccat ttcgtggata
     3421 tgtagagcat ctctataatc actcagcaat cattcgtatt gaaaacacga tggaatgcga
     3481 taaatggaca gcgaaaagca aagagaattt agcagtggtg agattggtgg atatggagat
     3541 tataaaaaca gaattataag ttatataagg agaaatcaat atgaatgaca aatttatcaa
     3601 taaaaatgac ttagactcat tgttagttgg atatgtgcct aaacgctatc tgactcaaaa
     3661 agaagcagtt cattacacgg ggacatctgc aggaacgatc aatgagtggg taaaaaaagg
     3721 attgaaagta atcatattcg gtgaaaacag ccgtccaaaa tatgacatca aagatatcga
     3781 tgagtttatt gctaaatata aagtatgaag aattattgaa gtagctaata gagaattcct
     3841 tatcagcata atattgttaa taaacataaa cagcaagcca ttattagctc gctgctcgtg
     3901 acaaatatga tttgcccctg ccaaggtaag tctattatat cacaggaggc agctatggag
     3961 ttaaaggtaa ttggattaag cgacattgaa aaaatgcaag gagaacattg tttaatcatt
     4021 atttcaaacg gtcaaatgaa aagtgtcgag cttccttcgt ttggaacaat agttatagaa
     4081 tcccattgca ataaagtcaa gcaagttaaa gaagaagtga aacaattatt ttaaatatcg
     4141 tcctaccaga aaactggcgg acacaagttg acaagaactt tcttgttgat ttgtgtccgc
     4201 ttttttgttt gcgaaattat tgaggaggct atctatatga atgatttaat tcaggaatac
     4261 aagaatgact tgaaaatgtt gaaaaagcaa catcaaaaaa tcttaaataa acgatatcgc
     4321 acacctgtca aagaacatgg gagagtgata cataaattaa ttgatgatcg ttctcctcaa
     4381 gatatagcag atcaaaaagt aatagcagaa gctatatcaa caacagaata tgctttattt
     4441 tggctagaaa cagggagaga aaaaccattt gatgatgaac aagctaagaa gataccaaaa
     4501 cataggagag ccgttaaagt ggcagatatc gatgttatga gttatcaagt ttacttgcaa
     4561 gaaatagaaa aacctgctga agagacgatt tccccaaaga aaagagaaat gttgctccaa
     4621 gtgacggaga tagaatcttt attgtctaat aaagaactga cattattcca tctgatcaat
     4681 aaagatctat gcacttatgg agaagctgct gaaaaaatgg acttagctgt tggaacagtc
     4741 aaatccatgt ctcagaggat aaaaaataaa atcgataatt attttgaata tggacaccaa
     4801 atcaaccttt ttgagattag ctaaaatttg taaaccattc ccacctataa gtgaagtcaa
     4861 ttattagatt agacactcac aagttaattc attcttttat tccgaataat tgttcttcaa
     4921 aaataaatca tgcatcgttt tcgatgcttc ttctttaacg atagtcagtg gcaaccaaca
     4981 taaataaaaa ggatgtgaac tcaactcctc ctaaaacgtt cttattatct atttgttgtc
     5041 actggctatt tcatacatat tatagggagg caagctgatg aggaactact ggtatgtgtc
     5101 attgatcaac gattatccaa gatgcaaaga ttgtcgtgat aaaagagttg ttcaatctgt
     5161 tcaaatgaaa agcaaatatt ctgttataga gatgatcaga gaagccgaac cgaatgaaat
     5221 tgatgcatgc cgtttagtct attgcggtca tggtttctat tcagataaac acatacagga
     5281 aaatctttca aagtatattt aagtacaatc aagaaactga gaggtggtga gaatggttga
     5341 actaaaaaat aataaacaag aacaatttgc aaaagaatat ctaattgatc ttaatgcaac
     5401 gcaagcggcc attcgagccg gatattccag caaaactgca tattctcaag gacaacggtt
     5461 gttgaaaaat gttgaaattc aaactcgtat ttctgaatta atgaatgaaa gaagtcgtag
     5521 aacagaaata actgctgatc gtgtgatcca agaaatagct aaaattgctt tttcggacat
     5581 cggagaatat atgacttggg atcaagatgg aaatataacc atgctttcaa gtgataattt
     5641 agatacgagt gtaattcaag ctataaaaag taatcgaact ttacgaccga ttggcgatgg
     5701 agaagaagtt atagattcat ctttagaagt gaagtttcat cctaaactta aagctcttga
     5761 gttgctttat aatcacgtta atggttcaga caaactatct aaggctcagg ttagaaaggc
     5821 aattgctgaa gcaaaaataa ttgaaaataa agcagataag ttaactgctg gaggaaaagt
     5881 caataatctg cttcaagctc tcttagatgt taaatctgga ggtgatggaa gtaatggaac
     5941 tgaagtttag cccgaaacag attagtaata ttaatcaagt ggttcaaggg attacttttg
     6001 aattgaatga aggaacacct cgtagtggga agacaacttc cgatattttt aaaatggctg
     6061 atttttattt gagatcccct gatcaaaacc atcttgtaac tgcttataac caggaacaag
     6121 cttatcgaat gtttatggat ggagatggat taggcttggt tcatatattt gatggttgtt
     6181 ccgatatacg acacgatgaa catggtgatc atctgttgtt atatgctcca aacggtgaaa
     6241 agaaaattta ttataaaggt ggagggaaga taaactctgt tggggctatc acgggtatgt
     6301 ctttaggaac ggtgacattt ctcgaattta atctattgca caaagacttt atcaatgaat
     6361 gctttagacg gacatttgcc gctgaatggc gttaccactt aggagaacag aacccaccag
     6421 cgccaaacca tcctaatctt gaattgcttg aacgttttga gaagtcagga cgttttttat
     6481 ttcgtcattg gacaccgaac gacaatccaa ttttaagtga ggagcgaaag aaagaacttt
     6541 ttgatgagct ttctagtagt gaatatcttc tagaacgtga ctggtacgga catcgagtat
     6601 taccgcaagg tgttatctat gctatgtttg gcaagaacaa caaagctacc gagataaagg
     6661 ggaatataat tgaaacattt tttactgcag atggtgggca agctgatgct acgacttgtg
     6721 ctttttgggt ggttactcat cacgaaggaa agttttattt gtatcgttta gctaactact
     6781 atcatagcgg aactgagact ggagaaacca aggcgatgtc tatatacgct aaagaaatca
     6841 aaaagtttgt cgagtggtgc tatacaaaat ggaaacatct tcctcgatgg aactggttct
     6901 ttgtcgatcc agcatgtaaa acactaagaa cagaactgga tttaattggt attgagactg
     6961 ataaagctga taataatagt tctgacaaag tttcgagtaa tggattgaaa atcgaagtcg
     7021 gcattgaacg tctccaaaat acaatgacta gtggacaatt cattatcttg gaaaacggag
     7081 aagaatatga ccactataat tttgaaaaag aaatatctat gtacgtaaga aatgacaatg
     7141 gattaccgat tgataaatat aatcacgctc tcgatgaagc gagatatgga aacaattatt
     7201 tctacaaaac ttacatcgcc tagaaaagag gtggacaaat gttcgataaa ttaaaagctt
     7261 tgtttaggat tggaggtgca aaaataggaa tggttgaaac attgaatagt atcacagatc
     7321 atccaaagat tgctatgagt gatagcgaat taagtcgaat aaggaacaat aaagaaattt
     7381 atagaaacgt ttatggggac atagaatata tcaatagtga tggttatagg cagacgcgtc
     7441 cttttcactc gttgaacgta tccaaagtag tgtccagaaa gttatctaag ctagtattca
     7501 acgatggatg caacatcagt ttggatgatg aaaaagctga tgaatttttg cagtcggtat
     7561 ttgctgacaa taaattcagg aaaaacttcg gagaagagct agaggctggt tatgccattg
     7621 gcggtttagc tttgaggcct tacgtggata ccaattcagg taaaatcaaa atttcgtttt
     7681 gtcgagctga tacatttttc cctctacaat ccaataccaa tgatatttca gaagcagcca
     7741 tcgctactgt aactcagcaa gccgagggac aaaagacaat ctattatact ttgctagaat
     7801 ttcatgaatg ggttgacgga aagtaccgca tcagaaacga attgtatcga tctgaagaac
     7861 aaaagcaagt tggtgtgagg atccctctca attctttaga aaagtacaaa aatctgcagg
     7921 aggagacgat cttagatggt tttagtcgtc ctctttttgt gtatataaag ctagcgggta
     7981 aaaataatat taatttagat agcccactaa gtttgggtgt gatcgacaat gctaagcgac
     8041 aattggcaga tatcaatgaa aaatacgatg agtttatgtg ggaaatagaa gaagctagaa
     8101 gaaaaatttt agcatctgat cactttttca gagttaaata tgacagcaat ggaaaaccag
     8161 tgaagcggtt tgacagtaaa acatctgttt ttcaacggct taaatctgat gaacctttta
     8221 ttgatgaatt cgctccatcg ttgcgatcaa ctgaatttat agcaagtatc aatttcattt
     8281 tgcgaattat tgagctccag acaggctttt ctagtggaac atttagtttt gatggccagt
     8341 ctgttaaaac agcgactgag ataattagtg aaaactctga aactttttct actcggtcag
     8401 ataatgttct tattgtagaa gaagcgttga aagaattgat cactacgatt tttgaacttg
     8461 ctgaggcata caagttattt aatcctgtca aagaattagg aataaatatc gactttgatg
     8521 atggtgtttt ccaatcgcaa gatgccaagg ctgattatta ctctaaacta gtaacagctg
     8581 gcttaacatc taagttaaat gctattcaaa aacttactgg agttacagaa aaagaagcaa
     8641 aaagggtagt atatgagatt agggctgaaa ctcttgatat ggattactca gagcatgagc
     8701 aaaacataat tgagggacaa ttaggaagtg aagaataatg gtttctccac atcagttaga
     8761 cttatggtct tctaatatgg ctcatctata tcaatcgtta gaaggcgaat taatacgtat
     8821 tatcatcaaa cgattgaata gtgggcacga taatatttta gattggcaaa gagaaaaact
     8881 gcaacagttg catctattta ataaagaaac tgcaaaagtg atttctcaaa taacaggaat
     8941 tgctgaatct gaaattgaaa gtatgtttga tagctcagga gaaaagataa tcagagactt
     9001 agacaaacaa ctaccttacg atcctaagcc tttgccatcg aacctagaca atgtcatgaa
     9061 agcttatcat gatcaagtat ggtctgacat aaacaattat gtgaatcaaa cgttactatc
     9121 taccaatttt ggttacggaa cagctaccac tcaaatgtac acagaaataa ttaacaaaac
     9181 gactgctgca tttaacagcg gtctttttac attcgatgaa gcactagaaa gaacgattca
     9241 aggatgggct caaaaaggta ttaagtctac ttttattgat aagggagggc atacatggag
     9301 cttagatcgg tatgttagag cagtcttaaa gtctaccctg tcaaatacct atgacgcatt
     9361 aagaaaagac cgcatgagcg agtatagtgt ccacacagtg ctagtcacaa gccatatggg
     9421 agcaaggcaa gcatgttcaa agattcaagg tcatgtggtt gatttacggc ccgtatctga
     9481 attgcctctt aattggaaat atagaagtat ctacgacccg tattggcaag cagaatatgg
     9541 aactgcaggc ggtcatcgag gtgtagaatg tcaacatctg catatcccat ttattcctgg
     9601 tgttaataca aataatcagc caaaatttga tgcaaaagaa aataaaaagg ttgcagaatt
     9661 aactaagaag caacgctacc tagaacgtca gattgtgaaa tataaaaaga atagaatggt
     9721 ctcagaagct cttgaacaag acgaaaatgc aaaagagtgg gcgaagaaaa ttagagtcac
     9781 acaaagtcgg ttgcgcactc tagttgattc tgatgagtat ttaagtagaa attacgcaag
     9841 agagaaggta tacacaccta ttaatacctt attgaaagat tttcactatg acgattttta
     9901 agtctaatca acgattaggc tttttatttt gcctagacct gctcggatgt ctctaaaaga
     9961 cggctcgcag tgggagttgc cactctaaaa aacacttagg aggaaaagaa aatgaaaaaa
    10021 gaagatctta tcgctttagg aattgacgaa gaaattgcta aatcagttat ggctttacat
    10081 gggaaaactg ttacgcagtt aaatgctcaa gtagctactg cagaacaaga gcgtgatcag
    10141 ttcaaagaac agcttgactc taaccagact gaattagacg cactcaaaga agctgcaaaa
    10201 ggtaatgaag gcttgactca acaacttgca gatttacaaa gtaaatttga tgctgccaaa
    10261 tctgagtctg aaacaaaact tgcagagcag cagaaagatt tcgctatcaa gttagcttta
    10321 aaagaagcga atgcgcttga tgagaacatc gtcttaggtc ttttagatcg agatactatc
    10381 aaggttactg atgaaggatt gcaaggtcta gatgaacagt taaaaagctt acaagaaaac
    10441 aaaaaatttt tgtttaatgc aatagataat tctaatccaa cacctcaaat tgttatgcct
    10501 ggaaatccta aaaatgatca gaagacttct ggtccttctt tagtagaaac tcaagcagaa
    10561 ttaaataaat ttagaatcac ccaataatta ttaggagctg acaaaatgaa aaaaaataac
    10621 ttatttaaaa tgaacttaca atattttgca aatgccgaaa ctacttttaa tccagacgat
    10681 gtgacaatgc aaaccgctaa aacgggctct atcccaatta accaatctac agaaatcatc
    10741 acagaagtta aaaatggatc tgcagcaatg cgactagcta aagctgttcc aatggctaaa
    10801 ccagtcgaag aatttacatt tatgactggt gtcggtgctt attgggttgg cgaaggtgaa
    10861 cgaattaata ctagtaaacc tactttcgct aaagcgacaa tgacttctaa aaaaatggct
    10921 gtaattattc ctactaccaa agaaaacttg aattactccg taacaaattt ctttgagcta
    10981 atgaaaccag aaattgcaga agctttttat aaaaaatttg accaagctgt atttggcgga
    11041 gtcgaaaatc cttatcaatt cagcgtacta aaagccgcta cagatgcagg gaatgtgatt
    11101 acagaaactg caaataaata tgatgatttt aacgatgcta ttgctgctat tgaagaaaat
    11161 gacttagagc caaatgggat cgcatctact agagcgcaac gtcgtaaata tagagctaca
    11221 aaggacggaa atggattgcc gatttttaat acagcaaatt ctaacggtgt agatgatatt
    11281 cttggattac ctattgctta tactccgaaa ggatcatttg gcacgggaga tacagcgccg
    11341 gtagagctag ttggtgattg gaattatgct tactatggaa ttctaaaagg gatcgaatat
    11401 gaaattttga ctgaggctac tttaacaact gtggttgatg gaaatgggaa cccaatcaat
    11461 ttggcagagc gtgacatggc tgctattaaa gcaacttttg agttaggatt catgactgtc
    11521 aaagatgagg catttgctgt agtaaaaaaa gcgtagcccc ttcaagcgtg acgttgaaca
    11581 aaacagctct aacgcttgaa gtaggggcga ctgaaacatt aacagcaact gtttagcctg
    11641 aaaacgcagc tgataaatct gttcagtttt cttctagcga tacagcaatt gctactgtaa
    11701 ctcctgtaca aggaaaagtc acaggtgttg ccgcaggaac agcaacgatt actggaacaa
    11761 cagtaaatgg taagacagca acttgtgaag ttactgtaac agaagcagga ggaggggcat
    11821 agttccctct tcttttaata aggagggata ttatatgcct tatattgaat ttgaagaatt
    11881 caaagatctt actgggaaaa cagatgattt taaagctgct ttcgaaaatc atttgtcaaa
    11941 agcaactgct attctagata gcatcactaa ctatttttat caatttaata aaattgagga
    12001 agatccaatt ggttttcgtg taaaacaatt taagctagct ttatgttcac aaattatata
    12061 tttcgatgaa gttggagcag atacttatga aagtatcaat aatacaccac aaagcttttc
    12121 tgttggtaga acgagtatct caaactctag tagatataat ccttcaggaa aaaatgagag
    12181 taaatcactt gttgccgagg atatctatat ttatttagaa ggaacgggtt tgttatatcg
    12241 aggtgtacca tcatggtaat gcctaaacct ccagtacaat ttctagtgga ttcttttatt
    12301 tatcgagaat atttaggaga aggagagtat aaccaaccta tttatggaga ttatgtaact
    12361 atagaaaatt gtcggattga ccgaggaagt cagtattctt tttcaccaag tggcaagcag
    12421 ttgctctata atgcagtaat tttttgttat aagactttaa ctaccccttt accgaatttc
    12481 aaagaacaat cattagttat ttatgatggt aaagaacatg tcatcactaa gatcgaaaag
    12541 attacagaag cgtattcaga tgctatctat tcatacgaat tagaggtgat ttgatgagta
    12601 ttaaagttaa tcttgatgga gttagagcta aagtcagccc acaggctatg aagcgaggaa
    12661 gatacgcatt agccaaccaa gcaatggctg acatgaactc atttgtacct aaaaagaata
    12721 atatccttcg ccaaagtgcg catatcaaaa gtgacggcag cgctattctg tatgaaacga
    12781 aatacgcaag gagacagttc tatctaaatg gaaaaaaata tactactcca ggaacaggtc
    12841 caagatggga tcttaaagca aaatcactgt atatgccttc ttggaaaaaa gcgtatctga
    12901 aaggagctgg tatccaataa tggattttat agatcggata aaagataaga ttaatagtat
    12961 tccagaactg ccgttaaaaa tgaaaaaagg ctatctttct gctgacgaaa gcttagtaat
    13021 ttacccatta ccaggcggac aaaatcttgt ggaatactat gacggtatta aagatgtaca
    13081 actaaatttt gaaattgcga tgaagtcaaa agatggtcat agaattgagc aaacgctttg
    13141 gcttatctct gattcgttag agcgtgtgtc agacgttgct agctctgatg gatcttttga
    13201 atttaacaat ttaactataa cgagcaaacc tttcatcaat gatgctgatg aacaaggttg
    13261 gttcgttttt ttattagatt ttcaaacaaa attaaccaca tttgaggggg aaaataaatg
    13321 agacgcaaaa atgccttaca aagctatttt attcaattaa ttacaactac taatgctgat
    13381 acaccaagcg aagacggctg gttggagtta gcaaagtgga tttccaatgt tgatgataat
    13441 tcgaacgaag aatctgaaac tactggctac tatgatggga acggcgaagg ggagacagac
    13501 gttacttctc atcagttagg ttattcattt acaggtttat acgatgaaga tgatgctgcg
    13561 atggcggcta ttgaggccat gattggaaaa tcaggagatg ctcggaaagt gtggttcaaa
    13621 gtagtatctg catctggtaa aaaacaacgt attggtaaag caacagtaac agagccagtt
    13681 gctcaggtcg gcgatgctac tgcatacggt gatttttcat gcggtatcgc atttgatagc
    13741 acaccagaag caaaagacgt ccctgttgca ccctaatgca ccccagaacg taactggggt
    13801 tttgaatgat ggatcgattt ctctttcttg ggatgccgta ccaaaagcac aggcctatgt
    13861 tatacactat agcaatgcta atcaatctga tcctcatgat gcaacaatga tgggatattc
    13921 ggaaaaaacc tcatggacgt tagcggctga agatgttccg acattagagc caggaaataa
    13981 aatttatctg tatgttcaag cgtataacgt tttaggaaaa ggaaaagatg aaatcgaaaa
    14041 agcgcgatat ttacacgatg gtccattcat aggttctgct tggtccaaat cagtagtatt
    14101 aatcaaaaaa taagccgaac tgtccgtctg ggcagctttt ttaggaggat aacatgacta
    14161 acaaactatc atttcaaata gagaaaaaag ggtttcctat caatattgga gaagtagagt
    14221 tctttttcgg tactactcca gaagaactga cacggttttt tgatacccaa gctgaatttg
    14281 aggaacaggt taaggaactc aaacaacaac ttaaacaaat caagaatatc gaacaaccag
    14341 aaaaggaaga tgctattaag attattgatc taacaaaaag tttagctaaa gcagaatatg
    14401 attcgttgct tggtaaagga tcatttgaaa aaatttattc tgtttatcgt gatgttgagc
    14461 aattaataga cttgtttgat ccgatttctt ttgaagttgc ggaagcaatt gaaaaagaag
    14521 ctttgaaacg taaagatact ctttctaaga agaaagctga tttattgaaa aagaaagcat
    14581 tgaaaaataa gaaaaagaag taggtgatta aatgcggtta aatgacccgt tagtcacttc
    14641 gatagagttt gatggtatag aattacctat cgatttaaca tttgataatg tactagatgt
    14701 ttttgatatt ttggaagata gtgatttgtt tccagaagaa aaagtgaata tgtgtttaga
    14761 actgttgatc agtgactttg aaaaattttt tcaaggctcg tctgaacaac agtttttatt
    14821 atttaatcac attttagaaa attatatttc tgtaggagat agtgatggtg ttgaaactga
    14881 tcgtttaggc aatcctatgc ctaatgcagt taaagaaaaa aagaccatca gcctagttca
    14941 tgatgctaaa tacatctatg catcgttcag acaaattggc atcaatttat ttgaagagca
    15001 aggacggatg atgtgggaag aattccaagc gttgcttgag agtttgccag acgacaccat
    15061 tcttgctcga atcatccaga ttaggacatg ggaaccgagc aaaggagaat cagctaaaga
    15121 aaaagaacgc atgagaaagc tacaacaaaa atatgcattg cctgattcgg aggtaggtga
    15181 agacgatggc tgatggaaga gtagagatag aagttgatgt ggatggcaaa ggcgtcacca
    15241 tacttaacaa gggtcttgat cagttagaag gtaagagtaa taaagcagga gcctcaatta
    15301 aaaatttagt tgtttcccta ggcctcgtta aagttgcggc ggctgctttt aatgttttaa
    15361 agaattcgct agattccgca attagccgtt tcgatactat gcaaaaattc cctaaagtaa
    15421 tgaaagcttt aggttttagt gcagaggact ctcagagatc cattaacaaa ttatccgatg
    15481 gaattgatgg tttgccaact aaattagatg atgttgtagc aagtacacag caaatgacag
    15541 caatcactgg agatttagat agatccacag acactgtttt agcattaaac aatgcatttc
    15601 ttgcgtctgg cgcttcaaca gaagatgcca gtcgaggtat gcaacagtat aaccagatgc
    15661 tttcgactgg gcaagtcgac ttagagagct ggaaaacttt acaagaaacc atgccccttg
    15721 ctttgcaaaa aacagctgaa gcaatgggat ttgttgggaa atcagctcaa agagatctct
    15781 ataatgcgct taaagaagga acagtaacat ttgatcaatt ccaagataaa ctgattgagc
    15841 taggaactgg aacaggaatg ttggcaacct tggcaaagga gaattcgcta gggatcgcta
    15901 cttcattcgg aaatctaagt aatgctgttt ctaaaggtgt agctaatctt attactaagt
    15961 ttgatgaact ggtgcagaaa cttactggta aaacgattgc tcaaaatatt gatagtatga
    16021 aatctattat taataaatca tttgaagaaa tgtcaaaagt aatggacata ttgatagata
    16081 atactgatga tatgatttct gcttttaaag gcctattaga cattgtggaa ttgttagctc
    16141 cagcttttat tgcagccact ggagcgtatg taggcttcaa aacagcatta gctctaggta
    16201 cattaataag ttttgtagga aaaatctatg gagtgataac ggccctaggt agtatggtca
    16261 gcatgtttgg agtatctgga acagcttacg cattgttatc tgctattatt ccggctggtc
    16321 tgacggtatt tcaactactt tcaggtgtta taggagcagc agttgcagct ttcatctatt
    16381 tttataaaac aagtgaaacg tttcgaaatg gcataaataa aactattgaa gttgtaaaat
    16441 ctggactgat taagtcattt gaatatctaa aaggagtctt tatttctata ttacctacac
    16501 ttcaaaaagt agctgataca gtaggaaact atcttgttaa aggatttcaa aaagttgtcg
    16561 aagtcggatc agcaattgct tcagtagcgg taccagcttt ttttaatttt gttgatgcag
    16621 taaaaagaat tgtttcttct ggcatagaaa gatttggctc tactctttcg caaataggct
    16681 ctgtattgtc agggattttt tcttctggta tagaattggc aggtaatctg ctggaaaaat
    16741 ttggaggagc tcttggtaaa gtcggcggag cagtttctct agttatcggc attttgacta
    16801 aagtagcaat agcagcacta gggcttactg gtccattcgg attagcagtt tctttaatta
    16861 tttcatttat atctgcgtgg gctaaaactg gcgattttag tgcagatgga atcacaaaag
    16921 tgtttgatca attaagtgaa acaattagca acgtggcaga ttctatctct caatatctcc
    16981 cgcaaattat cgaaagtatt acttctgtga ttacaagtat tgtcgacaaa atagttgaaa
    17041 tgttaccgca actaacagag atagctatac agcttattca aacattgacg gatgcaattg
    17101 ttacttattt gcctaaattg attgaaatag ccacgaaaat aataactact attgtacaag
    17161 gcatttcatt ggctcttccc gcactgctgt tggctgcaac agagataatt acaaaactaa
    17221 tttctgcttt cgctgagctt ctaccaaaaa ttattgaagt tggcacaaat ttgttaacta
    17281 tgcttattca agggatcgta gcagcactac ctacgataat tgaagttgtc attcagatta
    17341 ttaatacact gattgatggg ttcttaacgg tactacctat gcttttagaa gttggattac
    17401 aaatcattac ttctctcgtg aatgcgataa taactgcatt gcctcaactt attgaagcat
    17461 ctaccgttat cgttactact ttgctaacaa caattattga agctttaccg acattaattt
    17521 ctgcaggaat acaaatgtta atggcgctta tcggaggtat aatttctatt cttccgttgt
    17581 taataaatgc tgctatccaa atcacaatgg ctttgattag tgcattaatc agtgcacttc
    17641 cgcaaattat tgctgctggc attcagctgt tattagcatt aatacaagga ataatctcga
    17701 tattaccaca gttggttgca gcggcaattc aaatcactat tgccctagtt aatgcgttga
    17761 ttagtgcttt gcctcaattg atatcagcgg gtattaaatt gattgttgcg ttggtagatg
    17821 gagtgatctc agtacttcct caattagtat cagctgctat tcaattaatg gctgctttgt
    17881 tcaaagcttt agtaggtgct ataccacaac ttttatcagc tggtgttcaa ttgatcaacg
    17941 cacttattag aggaatcttg agcttattgg gacaattatt atcagctgga gcgagattaa
    18001 ttactggatt attgagtacc atagctcagt ttcttggaca aatggtgaat gctggagcca
    18061 atttaattag aaacttagtt tctgggattc ttagtgtgat aggatcagta accagtgcag
    18121 tatctaatat aggaaactct ataatagata ctttgagtgg aattgattta tttgaaattg
    18181 gttcaaacat catacaagga ttaattaacg gtatcggttc aatggttgga gcagttgctt
    18241 ctaaaatttc tgaagtagcg ggaaacatta aagacaaaat aactggcgcg cttggaattc
    18301 attctccatc acgttggatg agagattacg ttggtaagtt tatccctcaa ggaatagctg
    18361 taggtatcga agcagatgcg aaatctgcat attcagcaat gaataagctt tctaatggtt
    18421 tgatgaattc tatcacgcca gaatcagctc ttggcacttc aaggatgggg atggcatctg
    18481 ttggatcaca gatagttaat aatacctaca acaatcagaa gcaatttgat gttgaaaagc
    18541 ttgcacaagt aattgcaaaa caaccagtac gggtctcaag ttatttggat ggaactttag
    18601 taggggatga tatggatcaa cgttttggaa aagtattgaa tcgtagatcg tacatgagag
    18661 gaggatagtt tagtgaatga gaaaactcgt gtatatcttg cattttctga tgaaattgtc
    18721 gaattgacaa ataattctta tctgagattg attgacatta atataggtat gccagtggca
    18781 aaaaatgagt ttgttgaatt ttctggcacg aatggaaagc gtctttcgaa cagctcgttt
    18841 gacgcttttc ctattactct ctcatttgat attcgaagta gagagcaatc aatgtttgac
    18901 ttagttttac aaaagacgga actccgcgaa ttgtttacta gagaaccaga attttatcta
    18961 atttacagca aagaaccagg caaaaaatat cgagtagttt atgattctat tgacgacgaa
    19021 agaaaaggca caatttacac aagatatact gtaaatctgg gagctatcag aggatattct
    19081 gaatcaatcg caactacttt aacggatttc aatctagagg aagaatggca attctcgcaa
    19141 ggtctagtgg cggaagatta caagtatacg catcagacta gtcacttcat tatttacaat
    19201 gccggaagct ttgaaattga tccacgtgaa cattatctgc ggatcgtatt agaaggagaa
    19261 tcagaaggta atgtaacgat ctttaataaa acgactggtg atcgatttat ttactatcca
    19321 tcactttcta cgaatctcgg acagacatta gtcttggatg gcgtgattcc aaaattgaac
    19381 ggtgtaagtt gtgggatcaa tacgaaccat ggcctgatta atttagttgc gggagtcaat
    19441 gaaatcgaga tccaaaatat tactcgcgtg aaatcttcat gggatttccg attcttgtat
    19501 aagtaggtga ttgagtgact gatttaatta ttcgagatta tgaacaaacc aaagaagaaa
    19561 tccttgtcgg gtatggcaag gattcttttt atgagaactg gcaacagaat gaaacttggg
    19621 agattggttt tacagttacc aataattgga caaatcaaga agtgtttgat ttggtggaat
    19681 acgagtcttc tgttttttat aacggacagg aatttgtgat taaagagatg acgcgtaaag
    19741 cagtcggaca gttgttgacg aaacaagtag ttgcgactca tatctattac acggttcaag
    19801 atggctatca atacaataag gtgactggta caagatctat tagtcagcta ctcacgcatg
    19861 tatttagtgc gggtagtcgt ggctttacat gggaagtcat tgatccaaat aaaaagttcc
    19921 tcaccgttga acaagaaaac ttcggcaatg ccaattattt gaagctgatc aatgagattt
    19981 tatctgacta taatgctgtt gtgataccaa acaacaaaca tttaactttt tatcctgcaa
    20041 gtgaatatgg ccaaaaaaca gaagaacaga ttcgctacaa atacaataca gacgaagttt
    20101 catttgatat tgatacgtat tccttgaaaa ctcaaataaa gggttatgga aaactgaaag
    20161 agggcgctaa cactgagaat cccaaagaca gtgattacgt ctttacacct atcacttata
    20221 cgagcccaga atcagaaaaa tggggcatta ggatacaaga tccagtcagt gatgaacgat
    20281 atacagtatc aggaaatatg ctagagagat taaaggcaga cctgcaggat tatccaagta
    20341 tctcaggatc cgtaacgctg aaatggaaga taagtccaag caaaggcgat catgttccat
    20401 ttatttatga acctttgaat atcaatacgt acattcaagt tgttggaatt aagacatatc
    20461 cagcgatacc aaataagcca cctgagatca tattgagtaa taataagaaa acgatgacgt
    20521 caatattagc tgaaatggcg cagaaaggag tggtctgatg ggattattaa aactattcag
    20581 taatcgcatt tctaaagagt ggaaagaaaa gtttaataaa aatattgact acctaagcaa
    20641 tctcgaaaag aaactgtctg atcaagacaa atcaacgaac agtcgaattg ataatttagt
    20701 gctgcattca ggtggcgaat cgccgaatga agtggtggat gcacgagtta acaataaagg
    20761 ggaagttttt gacacactgc atggcagact attagaacat gaaaatctgt cggacgaaca
    20821 aattagcgaa ctgaatacaa acatggatag tcaaaaagag caagttcaac aattgaacaa
    20881 gtctgtccaa cagattattg gtgggtatag tgagccaata aatatgtacg tttcaaaaaa
    20941 cggtagcgat atttctggtg atggatccga agaaaagcca tttttaacta ttcaaacagc
    21001 agtgaataat gtcccgctga ttacaacgtc tcaaattact atttgggtgg atagtggagc
    21061 ttacttagaa gatgtcatga ttagaaattt gaatttcaca tcatttttaa ttcgacctat
    21121 cgataatttt gacactattg atccattaaa atcagatttg cccgttaagg tacgatcaat
    21181 ttgttttacg gcatgtaaag gctattgtca agttgctgga atgcaaattg tggatacagc
    21241 aaacggagca gactatggaa tcagaaatga gcaaagtggc tatatggcaa tcaatagatg
    21301 taaattcgca gagaacacaa aaacacttgc aagatataat gcagtttatg tcggcggaac
    21361 atcaaagatt aatatgtacg gagacacgac gttgattaac cagaaagtgg caatttacgc
    21421 tgtattgatg ggtgaaattt ttgtcagtgc gtttggttca ggaaacgacg taggaatttt
    21481 gtgcgagaat ggcactgtac gaggaactgt atcaacttct tttgcgacta caccaaccaa
    21541 aatttctggt tatggactag ttatcacgaa aggcacggtg ttgcaataat ggtatacaaa
    21601 acaaatgaat cgatcattgt gattcaagca gaagccacta gtccaaacag gacgaatgtt
    21661 gttttttggt cgcatgatcg aggaacagct aagcttcgaa tgaagttagt tcggaaaaac
    21721 ggcatccctc aaagtctgcc agaaggaaca accgttccaa ttcgcttgat gttcaaatct
    21781 gcaacggcag aaggtggtta tggtaaacat gactatctag ctacggtaga agatcctgtg
    21841 aatgggattg tttctattgt gttagaggat aatatactgg gatacgtagg caccgtagaa
    21901 ggtagcgtat atattgattt tccaaacgac cgctcgttag atacagctgg tcgttttact
    21961 ttttatatca aacgcagtcc gattgatgat agtacgccag agctggaaga ttattatttc
    22021 aatggtttca gtcagactat tgataaaatc gaaaaaattc tagctgatgg aaagcaagag
    22081 attgaacaga aaattacgga atctgaaacg cagattgatg cgaaattaaa agacacaaac
    22141 gacaaaatca cgaaagccaa tcaagatgtc gcaactatca atacgaatat tgataaagca
    22201 aatga
//
LOCUS       DACEOM010000054        21239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEOM010000054 DACEOM010000000
VERSION     DACEOM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.055"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..635)
                     /locus_tag="IW781_001957"
     CDS             complement(24..635)
                     /locus_tag="IW781_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1182952.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(758..883)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(965..1681)
                     /locus_tag="IW781_001958"
     CDS             complement(965..1681)
                     /locus_tag="IW781_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1182953.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1687..2256)
                     /gene="scpB"
                     /locus_tag="IW781_001959"
     CDS             complement(1687..2256)
                     /gene="scpB"
                     /locus_tag="IW781_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1182954.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2240..3037)
                     /locus_tag="IW781_001960"
     CDS             complement(2240..3037)
                     /locus_tag="IW781_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1182955.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3007..3378)
                     /locus_tag="IW781_001961"
     CDS             complement(3007..3378)
                     /locus_tag="IW781_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1182956.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3397..4284)
                     /gene="xerD"
                     /locus_tag="IW781_001962"
     CDS             complement(3397..4284)
                     /gene="xerD"
                     /locus_tag="IW781_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1182957.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4380..4853)
                     /locus_tag="IW781_001963"
     CDS             complement(4380..4853)
                     /locus_tag="IW781_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1182958.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(4958..5821)
                     /locus_tag="IW781_001964"
     CDS             complement(4958..5821)
                     /locus_tag="IW781_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1182959.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6017..8116)
                     /locus_tag="IW781_001965"
     CDS             complement(6017..8116)
                     /locus_tag="IW781_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1182960.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8336..9445)
                     /gene="rpoD"
                     /locus_tag="IW781_001966"
     CDS             complement(8336..9445)
                     /gene="rpoD"
                     /locus_tag="IW781_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1182961.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9469..11310)
                     /locus_tag="IW781_001967"
     CDS             complement(9469..11310)
                     /locus_tag="IW781_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1182962.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11492..13885)
                     /locus_tag="IW781_001968"
     CDS             complement(11492..13885)
                     /locus_tag="IW781_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1182963.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(13992..14852)
                     /locus_tag="IW781_001969"
     CDS             complement(13992..14852)
                     /locus_tag="IW781_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1182964.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14911..15543)
                     /locus_tag="IW781_001970"
     CDS             complement(14911..15543)
                     /locus_tag="IW781_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1182965.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            15752..16471
                     /locus_tag="IW781_001971"
     CDS             15752..16471
                     /locus_tag="IW781_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1182966.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            16497..19088
                     /gene="mprF"
                     /locus_tag="IW781_001972"
     CDS             16497..19088
                     /gene="mprF"
                     /locus_tag="IW781_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1182967.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19276..20463)
                     /gene="tuf"
                     /locus_tag="IW781_001973"
     CDS             complement(19276..20463)
                     /gene="tuf"
                     /locus_tag="IW781_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1182968.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20831..21187
                     /locus_tag="IW781_001974"
     CDS             20831..21187
                     /locus_tag="IW781_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1182969.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         5811 a         4459 c         3726 g         7243 t
ORIGIN      
        1 aaatttcttg atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt
       61 tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg
      121 atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac
      181 atgcccagca tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg
      241 aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa
      301 aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta
      361 aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct
      421 gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg
      481 aataagaaca tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa
      541 gccggcatga tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct
      601 actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct
      661 cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat
      721 agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta
      781 tactgtcggt atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact
      841 ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt
      901 tacatattca ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa
      961 gtgttcattt tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat
     1021 ccccaggacg taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca
     1081 caggaagacc aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg
     1141 tcaattccac gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg
     1201 gagatgtttt tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg
     1261 tagcgatccc ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca
     1321 ggcgctgagc aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca
     1381 gtcgacctac aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc
     1441 ttcctttgtc atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac
     1501 cgtattcttc ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt
     1561 ttatccctaa ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt
     1621 cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca
     1681 ttacaattat tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc
     1741 aagtgaaatt tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata
     1801 atctaagaag tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc
     1861 tttttcttca atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc
     1921 tcgaatctgc tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt
     1981 ttcgacggcg gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg
     2041 agccagctct tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc
     2101 tgagtgcgag ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc
     2161 ttcgctgaga tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat
     2221 aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat
     2281 aaagcataat tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca
     2341 attctagcaa agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag
     2401 aatcgaaagt acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt
     2461 cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac
     2521 gttctaagat tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca
     2581 acggcttttc agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac
     2641 ttcgttcctc tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta
     2701 aaagctgagt gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtga
     2761 cgaattcttg gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca
     2821 ctaagtattc tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat
     2881 attgctccgt gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca
     2941 gatgaagcag cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca
     3001 actcattcac tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat
     3061 ttcagttgca cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa
     3121 gcccatattc tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc
     3181 gttcgttgcc cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa
     3241 gaaaaaaagt tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt
     3301 tttacctgtc agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt
     3361 ccggtaatgt gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt
     3421 ataaacatcc gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc
     3481 cgcgtgacct aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt
     3541 ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac
     3601 taactgcttc agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac
     3661 aaatacatgc atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg
     3721 ttccagccat tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc
     3781 aactgtctgt accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc
     3841 actgacgcgc atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa
     3901 cgtttttgtc gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact
     3961 tggtaatttc tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat
     4021 acgctcttgc cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt
     4081 tgctgtcgct tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc
     4141 gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc
     4201 acgttcatag ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag
     4261 ataatcgttg atttgctctt tcataataat ccccctcatg cctctctatc atatcagata
     4321 ccgcttcatt ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact
     4381 cagacggact ggtttctttt ttctttttca tagcattcct gacagattcc atggaacgtt
     4441 agccgatgat cttttactaa aaaatgataa cgttcttcta ctacttgctc cacttcaagt
     4501 aaaagatcct cttctacttc ctcgatcgtg ccgcagttca agcatagtaa atgatgatgg
     4561 aaatgtttgg cgccttcttt tctcaaatca taacgggcta atccatcatt aaaacttact
     4621 ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc gataaacagt agcaagtcca
     4681 atttccggac tctttttttt cactaaaaag aagatttctt ctgcagataa atgctctttc
     4741 tcattttcca aaagcacaag cagagtagct tctctttgcg gtgtcagctt aaaccccgct
     4801 tcatgtagct gctgttttgt ttttttcatc gccgaaatag cattcatgac catttactca
     4861 accccaacaa tttataataa ttacaaataa aaaaagatat aacactatgt taattaaaat
     4921 tattataagc tagttaagaa aaacatgcaa gggattctca attagatttt ttgactagtt
     4981 ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa aagattacct aatgcgcgct
     5041 taaatgctcc tttgctgata ccaaaagctt tcatgatttc ttctggatcc gatttatctg
     5101 taaagttgat ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt tgtgcatcat
     5161 ctgatatagc ttcatatgct cttggcttca atgaaagatt cagtacaccg tctggtcgga
     5221 caccaatcac tcgacctttt atctgctcgc ctagacgagg ttcattatat cgttcagaag
     5281 gatggataaa cccaagatag aaatcttccg ttaaaacgta cgtacctgtt aatttcaagc
     5341 ggtatgctgt tcctatcaca tctttgtttt tcatttcttc tgttccatat ttgcttaaag
     5401 atttgaatat tttttcatct gctaaactgc cccaaatacg gtcttttgca tcaactctaa
     5461 gggaaatcat caagcgatcc ccttttttag gccatagctc tgtcattgtc ggcaattcat
     5521 caagagatac gacaaagtct ttatctggaa gaccgatatc gacaaacgca cctaaatctt
     5581 ttcgtgtagc agtaacgatt ccaaaagcgt aatgaccgat acggctttta ggaatttgtg
     5641 tagtgatccg attttcatgt ttttgatttt ggtaaccgaa tccttcaact gcttcaccga
     5701 ttttgtgttc accttctgat ttgcttaagc caaaggtttg tccgtttttt tggacaaagt
     5761 agtttttttc attttcatca ataataatac ctgtaaatat agttgctaat aaatcgttca
     5821 tttttttctc cctctatagt gaaaggtttt cttaatacat ttctttgcta tcataacata
     5881 atgttatcta cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg agtatgacaa
     5941 cagttgtgtc atactcccct ttttcgaata aacggtgtta aacggcaggc tcacagcctc
     6001 tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt ttacagttga
     6061 tggttacgat gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat gcagattttg
     6121 ttgaagtggc atatagatta taatagcctt ccccattttc gattctggct ttttctaatc
     6181 tgtaacctcc ttcaacgata tatccacgtt ggatacatgt agcaatcact ttgctttgga
     6241 cacctggtgc ccacgtccat gctggattag atgcatcggc aatatcactt gctctggcag
     6301 atttgattgg ctgattcgct gagtttgaag aggaaggggt cgaggatgcc gcttcttgct
     6361 gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc tgcagctgct gcttgagcgg
     6421 cttgttttgc ttgttcttca gctgcttttt tcgcttcttc tgcttttttc ttttcctctt
     6481 cttctttttt ctctttatca gtgagcatct tatctacttg tttcaattgt tcattcaact
     6541 gagcagatag atctgtattt ttcacttttt ctactgcttc ttttgccgtc ttgtattggt
     6601 cgcgtgttgc tgaatccact acttttccgt ctttaaagac tatagctgtt ttttctttag
     6661 ctgtttgcaa ttcagcatat tgatttttag cttctgtttg tgcttgttga atcaattttc
     6721 caaaatctgt gttatccgtt tcgattgctt ggatctcttt atctgctttc aacttgatgt
     6781 catctgctag atgatcatcg acgatggcag gtgctatgaa ccactcattc actttttgga
     6841 tatctcggat cttagattgg atatcttggt aagttgtttc taactctgaa tatccttttt
     6901 cattcttaag actggataaa tcatttttta gttttgataa atcttgatga atcatatctg
     6961 cacgaataaa ttgatggtga tcatctgtat aaaaggcagc aagtttctca tgaagttctt
     7021 ccatttgttg ctgtcttttt gcttctgctg ctacttcggc ttctactttt tgatgatggt
     7081 cataataagc ccagcttcct ccaccgagta gaagtactgc tgctaaaacg agataaggta
     7141 ctttttttga ttttttcttt tgcgctttat ctggatctgt tttttctaag tttgattctt
     7201 gatgtgtttt ttcagtatct ggcacgattg ttattgattt cgcttctgct tcaaacagct
     7261 taggctggtt ctcttctttt tctatttttg cttcaagatc agtaccctct ttgcttacag
     7321 attgattttc agcatcggat tcactattta tttcatcggg ctctgttgac tttttgtctg
     7381 tgttttcgag tatttctttt ttctctgctt ccggcagttt attcgttttt tccttttcta
     7441 attgtttttc agtaaatact tcagtttttt ctgtgatatc cgtttttacg tcttttattt
     7501 ttactgtttc tttacctgtg gcgttttctc tatcagagtc tttttcagtt tcttgttcct
     7561 tttctggctc tactttgttt tcctgtgcaa ttttttcact gatcattttg agcagctctt
     7621 tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat ataagcggat agtatcgaat
     7681 tttcttcctc attatcgacg atttctttca gttcagctac tcttcttttt tgttttgatt
     7741 caagcgtttc ttcttgattc tttcctgatt cttccgaagt ctcatgaaca gttgctttct
     7801 tctgttcatt tttctctata ttagttactt gatcgtgagc aggtggttct tcgtcttctg
     7861 gttctgcttt ttcgaataat tccattacag attctagagg taaatctttc aattccgacc
     7921 attcgattgg atcgtttaat tctgtatcag gaaagactgc atcttcagtt gatttatcct
     7981 gcatagctgc atctgtattt gtttcttcaa ttgattcttt tatttgctga tcttctgcag
     8041 ttatttgatg accgcagtct ggacagatag tattctgctc catttttttc gatccgcaat
     8101 ttggacattt tttcatcact tagcgatcgg ctcctttact aggtttacgc taccatttaa
     8161 tctatcatat tctatacgtc acgccaatag cttattttta ggatttactt aaaaataatg
     8221 aatgaatttc cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa gctttttttc
     8281 ttttaaaata aaccaaacag ttttactgat ttctcttctg gtcaatagtc cacgcttatt
     8341 ctaagaaatc ttttaattgt tttgaacgag aaggatggcg caattttctt aatgctttag
     8401 cctcgatctg acgaatacgt tcccgagtaa caccaaatac tttccctact tcttccaatg
     8461 ttctcgtccg gccatcatct aaaccaaaac gtaagcgaag gacattctct tcccgatcag
     8521 tcaatgtatc tagtacatct tccaattgtt cttttagtaa ctcataagct gcgtgttctg
     8581 ctggacttgt tgcttcctgg tcttctatga aatcacctaa gtgagagtca tcttcttcac
     8641 cgattggcgt ttcaagggaa acaggttctt gcgcgatttt caagatttct cggacttttt
     8701 ctgttggaag atccatttcc gcaccgattt cttctggcgt aggttctctt ccaaggtctt
     8761 gcagcaattg acgttggata cgtatcaatt tattaattgt ttcaaccata tgaactggaa
     8821 tacggatcgt acgtgcttgg tcagcgattg ctcgagtgat ggcttgacga atccaccatg
     8881 tagcataggt tgaaaactta aatcctttgc ggtagtcaaa tttttcaact gccttcatca
     8941 gtcccatgtt tccttcttgg atcaagtcaa ggaactgcat gcctcgtcct acataacgtt
     9001 tagcgatact tactactaaa cgaaggtttg cttctgccag acgctgtttc gcttcttggt
     9061 cgccttcttc gattttcaat gctaacgcta cttcttcttc cgcagttaaa agggagacac
     9121 ggccaatttc tttcagatac atacgaaccg ggtcattgat ttttacaccg gttggcgcag
     9181 aaagatcttc tgtttttgct tgttcagctt ttttctcagc actcttcaag ctgtgaattg
     9241 atggatcacc attttcatcc accacactga tacctgcgtc ttcaatcttt tggatcaatt
     9301 tgtccatttc atctgcattc aaagtgaatg gtgtagcaag ctgattggat aaatcatcat
     9361 atacaacttg tcgtttgggt ttgttttctt tgatgaaagc tgctactgct gcttcatacg
     9421 tttttttatc tgttccattt gccataaaag aaggcccccc ttgagtagct atttggcttg
     9481 ttttaactgt ttcgttaaat tgatgatttc aatcgtcaat tcaagctcaa gctgtttgtt
     9541 tccagttcgt ctggcttctt gctgcatttg tttttttaat gttatttcat cagctaagcc
     9601 agatttgctg atcacatcta gcaagtcttg gatttctcgt ggcgaacttt cttctgccac
     9661 cggtaaatat tcgatttcca ccaccaaccg ccttaaattt tcatcttgca aaaagtctaa
     9721 aaactctgca aggttgaatt cataatgttg tgagacatat gcatcgaata aaaaataaac
     9781 ttctgcatat gcatcatgaa taaactgaaa ttctcgttct tttaaaatat tacggacaga
     9841 aacttcttta aatgcacgat ataatagaat ctgctctgcc ttttctactt gcgtcaaagg
     9901 tctctttttc cgtgcagttt cttgtctcgt aacctgagtg tgttgacttt cctgaggttc
     9961 ttgacgattt tgctgatttg tccttcgaag atcgcgcagt tgtttttgta tcgtctctct
    10021 gccgatctga aactctgttg acagttgaga tagatacata tcttgttcga ttggtgaaac
    10081 aactcggcta agttcaacta gtaattcttc gaggtattcg atcttgtctt tttcattatc
    10141 taaatttttc ccttgtttta aataacgtga tttaaaagta aagactgttt cgcgcccatg
    10201 agccactaat tcttggaaag atgcttctcc atacttacgg agatattcat caggatcaag
    10261 cttttctgga acgactacga cagaaagtgc taatcgactg ttttgccgaa gcaattcaat
    10321 ccctcgattg gttgcttcaa aacctgcgtt gtctccgtca tagcaaaaga ccaattcttt
    10381 cgtcagcttt tccatttgtt ggatctgttg agctgttaga cttgtcccca tagaagctaa
    10441 tccagttttg ataccagcct gccatgcagc aatcacatcc ataaaccctt cgaacaacaa
    10501 aatttctccg tttttccgga tatcgcttct tgctttatca agattaaata aaacttgtct
    10561 tttattgaac agttctgttt ctggactatt caagtatttg ggctgatctt tttcctgccc
    10621 tttttcaggt gcaagccagc gtcctgaaaa accgatcgtc tggccttgag aattacgaag
    10681 tggaaacatc actctttgat aaaaacgatc agacatacgg ccatcatcat gcgtgacaaa
    10741 caatccagag cgttctaaca attcatttga aattgattca ttcttgaata cttgctgaag
    10801 aaaagaacgc tcattaggtg caaaacctat gttgaattct tcaattaatt ctaaagacaa
    10861 cccacgactt tgcaaatatt ctaaagctgg ctgtccagct tttgtatgca atagcatgtg
    10921 atgatagatt tccgctgctt tttcatgagc agcaatcagt ttccccgtag cagattcagg
    10981 attttggaca gctatagtac gctgacgcca ttgatcttct aacggtatct gttcgaagtc
    11041 tgccactttt accactgctt ctggaaaact tagaccttct agttcttgaa gaaaagtaaa
    11101 cacgtttccg cctttaccac aaccaaagca atgaaaaatc tgcttgtctt ctgcaacaga
    11161 aaaagacggt gtacgttctt catgaaaagg acacagcccc aagtaatttt taccagactt
    11221 tttcaactgg acatattgtc caatcacctc aacgatattc gtttgactgc gtactgtttc
    11281 gataacctct tgaggaatcc gttgagccat tttttcaccc cctgctctat tgatatcttt
    11341 tgggtttaac agaagatatt tcaggagaat aaccggtaaa aaatcgcact aactgaaatc
    11401 agtgcgattt taaaggacac aattatacat gtttgattgt atcatatttc attgcttttt
    11461 caagtataac acatttctat ctcattaagc atcaatttgc tactcttttc agtaactgat
    11521 tttgaatatc agccttccag tacaatactc gaagaacaat atttacgagc atgaatacaa
    11581 agaagtatcc tacgctctct cccaacccta agtttacttc caaaattcca tggaacagca
    11641 ttgcagctac atatatccct acgatcgtcg ttactgctag gaagatgcgc aatgggttga
    11701 agggataaca agcttttaca actgccatgc aactgatccc aatcagcaag taatacatca
    11761 aagtagtagt ttcagcattt ccccatgcga atgtttttcc aagtagatac accgcaatga
    11821 tattcactac tacaagcaag gcatttggta aagcattaat aagtgctgta ggcaaataac
    11881 ggtaagtgat ttttctttta tcttgttcaa aagataaaaa gaaggaagga tacccttcaa
    11941 tcgctaaatc aatcagcgtg atttggatag ggataaacgg aaatccaatt gctgtaacgg
    12001 cacatataat cgataaaata aatgaataaa tcgttttgat aaaaaagatg cctgagactt
    12061 tcgtcacatt gttgaccact cggcgtcctt caaacaggac ttctggcaat gtagtaaaat
    12121 cagagtccaa caataccaga ttcgcaatct gacgtgttgc tccatcacct tctgccatag
    12181 caatgctgca atctgcttct cttaacgcca aaacatcatt cacaccgtcg cctgtcattg
    12241 caaccgttct gccgctttct ttcaattcat ttaccagcaa tttcttttgc tgcggactca
    12301 ctcgtccaaa aaccgtatag cgatgagcag cttctcgcac ttccatttct tcagtaattt
    12361 gagaaagatc gacatacgca tcataattcg gtaatcctgc acgtctagcg atattggaga
    12421 cagtaaaagg attgtctcca gaaatcactt tcagatccac accttcctca tgaagataag
    12481 ctagtgtttc ttgagcgttt tttcggattg gatcatcgat ttcaaacaga gcaaccggct
    12541 caacaaatgc tggtttttct tcgttcaatt gctgatttgg agcgatcccc aacataagta
    12601 cgcgatagcc attcgcttgg gctgttttta gactgtccgg aagccgatct tcttccacca
    12661 aacgttcagg cgctcctacg tacacgactc ccagttctgg aaattccatc gcgccccatt
    12721 tgcgatcaga agaaaatgcg actttgtttg tcacatcata ccgattagaa agctcaaaat
    12781 aatctctcat agcccgcata gtgatgttgt tatcactgct ttctgccaga tacgtcccca
    12841 taatttctgg gatggctttc tcgtacagcg gatgtagtgc ttctacccgc tggatactca
    12901 ttttcccttc agtaatggtc cctgttttat ccaagcataa tgtatcgaca tgggccagcg
    12961 tttcaattga atacatgtct tgaacaagga ttcgtttttt tgccagtttg gttacccctg
    13021 tcgttaatgc aatacttatt agcaatacca aaccctttgg aagcatgcct aacaaagctg
    13081 ctgcagatgc aataacagaa attttgatag aagcatcgcg gatgaacagt gcttccaaaa
    13141 atagtatgat ccccaaagga ataatcacga aactagtgaa tttggatact ttgcgaatcg
    13201 aatctacaag ttcagaacga atagctttgt gagtctttgc ctcgctagtc aagcggacag
    13261 cataattatc tgcacctact cggataactt cagccatgac agaaccgctt gtcaaaaagc
    13321 ttccagaaag caattcagct ccagtttctt tgacaatcaa atcagattcg cctgtcaaaa
    13381 gagcttcatt tgcttctgcc cgtccagata taacctgtaa atcggctggt atctgttctc
    13441 ctgctccgat ttttaccaaa tctcctaata ccagctcttt agtagaaatc gattgttctt
    13501 taccgtcccg gatcactagt acttctgatt gggaaacaat cgataatttc cgtacaagat
    13561 ttcttgcatg tatttcttga taaattccaa taacgatatt gacgacaata attgctaaat
    13621 acgccatgtt ggaatacgca cctacaaata acagacaaat cccaatacca aagttcagaa
    13681 aattgaataa cgtcaatata ttatcgctga atatttgttt tgtacttttt gatgcatctt
    13741 cctggtagtc attttgctgg ccagaagcca ttttctgaac tacctcttgc tgtgtaagtc
    13801 cattcatttt ctttttctcc attttccgcc aactttcaaa aataatcacg tgtttaagtc
    13861 tagcgtgttc caagattaaa aacaacttgt ttttataaaa tttatagaaa aacacacgaa
    13921 tgattttaca aaaaagatgc caaccttttt gtttgaatca cccgtgtgct gtttttatta
    13981 tcaatctatc tttacttata ttgttcctcc aaacggctca tccaccaacc tagtgctgta
    14041 ccggctataa aaagaacagg acaagctagt accccagcaa ctcccaatcc cgcgtaatcg
    14101 gtaggaatga tcataatcgt tgaagcaaaa actacaccta aaatgaaatg gaatagcttg
    14161 ctatatgcct ttgagaaaat gtaatccatg atttttgaca atccaagaac acaaagaatc
    14221 ccaccaattc cgatcggcac aatcacttca agtcgtactt ctttaaatcc gtcagccatc
    14281 gctttataca atcccatata caccaaaaaa ttagaagggc tcaatcctgg gacgatcata
    14341 cccaaaccaa tcaatccgcc ggcaatcagc catgttccaa aattctgatc gacttgcccg
    14401 tcaaataatt gagcgccgta taataaaaac aaataagcca gaacaaaagt aacaaccatg
    14461 atgatcacat cagaagtatc tcttcctttt ttccctgctt ctttccataa cgcaggaaca
    14521 gttccagcga tacatccgac aaaaaaccat aatatcgttg tttcatacgc acctaataaa
    14581 aaactgacta aaaaagaaag tagaaagatg cctgtcactc cgcctagacc aacaggcaga
    14641 aaaaacaaaa cattttcttt gaaatttttt gtaaggtggg ctaaaaaaga aatcagccgc
    14701 tcatacatgc caaagacagc tgctaatgca ccaccgctga ctcctggcaa gataaatcct
    14761 gaaccgataa acattccctt cacaaaccgt aacatccagt ctttggttgt gtaccccact
    14821 tcttgatttt ccattttttg ccttttttcc actatttgcg cactccttct ttcatttaat
    14881 tttattcatg attgaacatt tcctgctatt ttacttaaag actttttcat aggtctctat
    14941 tattttttca ttcattttga caatatcagg ataaaacatg ttacctccat tatgatagag
    15001 atagccgcca ttgtaaagca aggactggat ccgccaatat cggtattgtt tcccttcctc
    15061 atttcctaaa agtgggctca gcacatcttt agaatacgct tctgctaatt ctactgtgtt
    15121 ttctccgcca tgatttttta cataggcaat ataatctttc ccaaaattat atgcttggac
    15181 agctgtccat aaatcgcatc cttgtttctt tgcttctact atcatttccg ccaaatattt
    15241 gacaccttga tcaatactgt cagctggatt atcaatgatc tcagcttctc catgcagact
    15301 ttccgagctc tgcatgagat ctgtcccttt tccttttgat tcggtcaata ttatcgcctt
    15361 gatcaaattc tcgtagtttt ccatatgata attttttgca gctgttgtta tttctatttg
    15421 atagctttcc actcgtttta ccgaacgata agttcttcct cctaaaaaca acaccgcacc
    15481 aataaccgtc agaatgatca tgtaaaaaag aatacggata atagataatc gttttcgctt
    15541 catttcatca cctagttatt ttgacatctt tttttatctt actatttctt tatcttcttg
    15601 gctatttctt ccttgatttc tgtgtacgcc ttaagaaaaa atgaattttc ctgtattttt
    15661 tgacatctta cgaaaatgat cgttgttttt cagtaaaaac aattgcccaa tgactgatat
    15721 aatcgttata ctttacacag taggtgatta tgtgaaaaaa gaaaagaaat atgcaaaaat
    15781 gccagatggg agcgaaattt actatgaaaa gagcggtcaa ggcttccctc tctttttact
    15841 gcatggcaat gacggcagcg gccgcttttt ttcagaacag gtccctgtac tcgaacgcta
    15901 ctataccgtg tatctagtag atagccgagg acatggtcgt tcgacaaacg aagcatctat
    15961 gttgaatttt cagttaatgg ccgaggatct gaatacgatc atgttgctgg aaaaaatcga
    16021 tcaggcagat tttcttggat ttagtgatgg agcaaatcta gcactggtat ttgccagcag
    16081 cttccctaaa aaagttcatc gattgatttt aaattctgga aacacgcttg ttaaaggcgt
    16141 tcgtttttct gccagagtca tcagtaatat tcattatgca tgggtatggt tactctcttt
    16201 atttcgccca agtcttcgca aaaatctttt agttatcaaa cttttgctgc atgacatcgg
    16261 gcttacagaa aatgatttaa aaaaaatcaa ctcccccact ttgatcatcg tgggaaaaaa
    16321 agatgtaatc aagctaaaac attctttgta tatagctaaa actataccaa aagcttcctt
    16381 tgtgcttgtg aaagaacaag gacatgaatt agctagaaaa gatcctgaac gcttcaatcg
    16441 agaagttctt caatttcttt ctgaaacata actattagga atagaaggtg atttatttgt
    16501 taaaaaatac catacaatgg ctgaaaaacc acctaggact gttcaaaaca atctttttga
    16561 tttctgtcat tgtcattatt gtcggtgaat tgatgtccat cggtaaaact ctatctatcc
    16621 agcaattagg agaaacgttt gccactatac ctgtatggaa aagcggacta atgctattga
    16681 ttggtcttat ctctgtgtta cctatggttg gttatgatat tattttgaat cggatgcttg
    16741 aacaaaagca gaatcctcgt tacctgtttg agacaagctg gctgatcaat acgatcaaca
    16801 atattgccgg tttcggcggg tttgttagtg tggggcttcg ctctgaacta tatggacaaa
    16861 agaaagagag cagaaacgtg atccaagcat tatcgaaagt tttcttgttt ttgatggctg
    16921 gactttctat ttacagtctg ctttcttttt tacttgtagt cttcacgcca gttgctcctt
    16981 ttttgaagca atattggata tggttgatcg gcggcggtct atattttcct gtcgtctttt
    17041 tatttactac tttcaaaaag aaaggattcc ttggtggtac ctcacttaaa actcaaggac
    17101 agctattgct agtttcattg ttggaatgga ccggtgtcct gtttagtttc ctttctgttg
    17161 gttatttaat ggaaatccgg attgatctgt ggcaaacaat tccattattc attgctgctt
    17221 cagtgatagg aatcgtgtct atgattccag gagaaattgg gagtttcgat gtaatgatga
    17281 ttatcgggtt gtccgctatt ggtgtgccaa gagaaatagt cgttgtgtgg atcttgctct
    17341 atcgcctgtt ctactacatc attccgtttt tgattggaat cgtctttttc ttcaaaaata
    17401 tcggttccac ttttgatcag cgttattctg gtatccctaa acaattagca acagaaattg
    17461 cgcataaaat cgtggttgtc ttattatact tttctggaat catgctagta ctttctgcca
    17521 ctatcccgca agcttttaca gagtttcgct ggcttcatag tttgaaccca ttgaaatttc
    17581 attttattat ccaatttcct agtatactat tgggatttct tttaatcatt atgggcagag
    17641 ggatcgcagc acgtgtgaaa cgagcatatt tgcctacaat cttcttgatt gttctagctt
    17701 tactttatgt tttattaagt gattttagtt tcacaccggt ggttttcttg tctattctgc
    17761 ttctgatcat tcttgcttct aaaaacgaat tatttcggga acaattgatc tattcatggg
    17821 agtggcgtac gatcgatgga atcattattg gtgcactgtc cttgctttac atcgttattg
    17881 gggtatacaa tctgccagat ttccctcatc ggcgtcacca ttttatttcc tttttcttat
    17941 ttccttctga aaagatttgg ttttcgggat tgttggccat catcgctgtt agtttcatga
    18001 tcgttctatt tgttcatttc cttcaaggag aaaagaaaca gattggcgaa gcctttaatg
    18061 aagaaaaggc cctgaagatc cttacaactt atggaggaaa ctccgatagt caattgatct
    18121 tcttaaaaga taagcggatg tttgcctatg aaaaagacgg tgaaccaact gttcttctcc
    18181 aattcgcttg cttcaataat aaatgtattg taatgggtga tccttctggt aagaaagaag
    18241 attttccaga agcaatcgaa gcttttatcg aagaaacaga tcgactatgc tatttaccag
    18301 ttttctacga aacaagcgaa gagattgtta tgattttgca tgaattcggc tacgatttca
    18361 ttaagatggg ggaagaagct tatgtagacc tgaatagttt tacaacttca gggaaaaaaa
    18421 tgaagggcac tcgtgctgtc ttgaatcgga tcgaacgaga aggatttaca tttgatgtct
    18481 tacaaccacc attttctact gagcaaatga gtactttcaa aaatatctct gataattggt
    18541 tgggttcacg aaaggaaaaa gggttttctt taggcttttt ctctgaagat tatctacaaa
    18601 gagcgcctat tgcagttgtc aaaaataccc aagaagaaat tgtcgctttt gcaacgatca
    18661 tgcctactta cacgaacaat aaagtaggaa cgattgatct gatgcgatat gattctaaaa
    18721 aagcaccttc tggcagtatg gatttcttgt tcttgcattt gtttgcctat atgaaagaag
    18781 aacatatcca atggttcaat ttagggatgg cccctctttc aaatgtcgga acttccagaa
    18841 agagtttttt acaggaaagg attgcatact tggtttacga gtttggttct catttttatt
    18901 ctttccatgg attaagagaa tataaaagca agtacgctac aaactgggtc tcacgttata
    18961 ctctatattc tagagatagt tggattgcat atgttatgat tgctttgctg attattgata
    19021 atgcgccagt tgagcagaca caaggtagaa taagtggttt gaaaaaatgg atacgaagaa
    19081 agtattaaaa actgcatacc gatagatgaa cactctcatg atgaataaaa gagactgaag
    19141 taaaacgaaa gttcatgctt ctacaatctc ttcttataca attgaagaac aaagagcggt
    19201 ccaaacagaa atcctgtttg gaccgctctt ttattttaaa catttttcat taccaaaata
    19261 ttcgagtcgc gtttcttatg cttcgatctc tgtaacgata cctgaaccta ctgtccgtcc
    19321 gccttcacga attgagaatg tcgttccgtt ttcaacagcg attggatgga tcaagtcaac
    19381 atcaatcgtc acgttgtcac caggcatgac catttctgta tcttctggca atgtgattgt
    19441 tcctgtaaca tcagttgtgc ggaagtagaa ctgtggacga tagttgttga agaatggagt
    19501 atgacgtcca ccttcttctt ttgtcaacac gtacacttct gctttgaatt ttgtatgtgg
    19561 agtaatggaa cctggtttag caagtacttg tccacgttcg atatcgtcac gttggatccc
    19621 ccgtaacaac acgcctacgt tatccccagc ttccccgtaa tctaacgttt tacggaacat
    19681 ttctacacct gttacaactg ctttttgtgt ttcaggtttg atccctacga tctctacctc
    19741 atcaccgaca cgaacagcac cacgatcaat acgacctgag gcaacagttc ctcgacctgt
    19801 aattgagaag acgtcttcca ctggcaatag taatggttta tctgtatcac gttctggtgt
    19861 tgggatatat tcatctactg tatccatcaa ttccataata gcagcttcag catctggatc
    19921 gccttgcaat gcttttaatg cggaaccttt gatcacagga gtgtcatcgc ctgggaaacc
    19981 atattcgctc aataactcgc gaacttccat ttctaccaaa tcgatcaatt cttcatcatc
    20041 gactaaatca actttgttca agaaaacaat caggtatttt acaccaactt ggcgtgacaa
    20101 taaaatgtgt tcacgtgttt gaggcatcgg accgtcagtt gcagaaacaa ctaagattgc
    20161 accatccatt tgtgctgccc ctgtaatcat gtttttcaca tagtccgcgt gtcctggtgc
    20221 atcaatatgc gcgtagtggc gtttttctgt ttcatattct acgtgtgcgg tattgatcgt
    20281 aatcccacgt tcacgttctt ctggagctgc atcaatacta gcatagtctt gaggatttgc
    20341 taggcctttt ttgcctaata ctgtagtgat cgcagcagtt aaagtagttt tcccatggtc
    20401 aacgtgtcca atcgtaccaa tgttaacgtg tggtttactt ctgtcataat gttcttttgc
    20461 cataatttac aaacctccta aataatatag atcactgaaa aatttcagct ctcacctttt
    20521 tattatagtc tttttttgac taaatcaaaa agaatacgtc cgacttactt tttgtaagat
    20581 tcgaactaag cttattatac gtcaatattg gtcaaagtca actaagagca ctcgtctgtc
    20641 ttatacgcga aaaaagtacc ttttataaat aaaaaaatgt gttttttgag aaaaacttca
    20701 agatagtcct taactttctt ttgaagcaat aagaacaaaa tgaggaaaag tttaaaattc
    20761 agctgttgcc aacaactttc aatagacgaa atcttctatt tcgttaataa taaggaaaga
    20821 ggtgaagtac atgaaaaaat taattggcgt acttgtcgtt ttaggcatat ttctcggcgg
    20881 aagttttgca gcttatcatt atttttacgg cggcgaagct tattatacaa aaatcacgac
    20941 gaatggcgaa atgtcagcta cccaagcaga taacggagag aaattcacga cttataccta
    21001 caagcaagcg gcttataata aagatggtga aaaaaaacag gtgacgcttc gcgaagaaag
    21061 agaacatcca ttaaagatga atgcgtatct taaactcaaa gtcaatcctc gtaaaggtgt
    21121 attaagctgg gaagaaataa aggcttctga agtgcctaaa aatgcggcag aaaaaatcga
    21181 taattagtaa atggtcttaa aattttatta gataaaaaga ggctgggaca gaagcgttt
//
LOCUS       DACEOM010000055        20836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEOM010000055 DACEOM010000000
VERSION     DACEOM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.056"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(48..1249)
                     /locus_tag="IW781_001975"
                     /pseudo
     CDS             complement(48..1249)
                     /locus_tag="IW781_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1366..1881
                     /locus_tag="IW781_001976"
     CDS             1366..1881
                     /locus_tag="IW781_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1182970.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2080..2526)
                     /locus_tag="IW781_001977"
     CDS             complement(2080..2526)
                     /locus_tag="IW781_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1182971.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2573..4051)
                     /gene="msr"
                     /locus_tag="IW781_001978"
     CDS             complement(2573..4051)
                     /gene="msr"
                     /locus_tag="IW781_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1182972.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4405..5034)
                     /locus_tag="IW781_001979"
     CDS             complement(4405..5034)
                     /locus_tag="IW781_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1182973.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5018..5482)
                     /locus_tag="IW781_001980"
     CDS             complement(5018..5482)
                     /locus_tag="IW781_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1182974.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5969..7237)
                     /locus_tag="IW781_001981"
     CDS             complement(5969..7237)
                     /locus_tag="IW781_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1182975.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7468..8205)
                     /locus_tag="IW781_001982"
     CDS             complement(7468..8205)
                     /locus_tag="IW781_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1182976.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8272..8841)
                     /locus_tag="IW781_001983"
     CDS             complement(8272..8841)
                     /locus_tag="IW781_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1182977.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8969..9841
                     /locus_tag="IW781_001984"
     CDS             8969..9841
                     /locus_tag="IW781_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1182978.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10183..11055
                     /locus_tag="IW781_001985"
     CDS             10183..11055
                     /locus_tag="IW781_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1182979.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11052..13010
                     /locus_tag="IW781_001986"
     CDS             11052..13010
                     /locus_tag="IW781_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1182980.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13261..14112)
                     /locus_tag="IW781_001987"
     CDS             complement(13261..14112)
                     /locus_tag="IW781_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1182981.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14235..14702
                     /locus_tag="IW781_001988"
     CDS             14235..14702
                     /locus_tag="IW781_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1182982.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14965..15447)
                     /locus_tag="IW781_001989"
     CDS             complement(14965..15447)
                     /locus_tag="IW781_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1182983.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15547..16410)
                     /locus_tag="IW781_001990"
     CDS             complement(15547..16410)
                     /locus_tag="IW781_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1182984.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17008..17856
                     /locus_tag="IW781_001991"
     CDS             17008..17856
                     /locus_tag="IW781_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182985.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18027..18368)
                     /locus_tag="IW781_001992"
     CDS             complement(18027..18368)
                     /locus_tag="IW781_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1182986.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18454..19161
                     /locus_tag="IW781_001993"
     CDS             18454..19161
                     /locus_tag="IW781_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1182987.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19148..19471
                     /locus_tag="IW781_001994"
     CDS             19148..19471
                     /locus_tag="IW781_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1182988.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19560..20597)
                     /locus_tag="IW781_001995"
     CDS             complement(19560..20597)
                     /locus_tag="IW781_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1182989.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         6208 a         4312 c         3698 g         6618 t
ORIGIN      
        1 gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta ttcttcttca
       61 tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag aacaatggca
      121 cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc tgcaataaat
      181 ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc aacgtttcgt
      241 tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat tccttggaaa
      301 aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc agctcctaat
      361 aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata agtcgtaaac
      421 ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac accccagcca
      481 aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg cgcatacgca
      541 agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga acgaagttta
      601 tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg tttctcagtt
      661 tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa
      721 aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc aacagccatt
      781 ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc aactaaaata
      841 ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc ccagccggca
      901 cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc gaatacaacg
      961 atgataaaca atcctgtcat caatgttttt tcaaaccgat ccgactagat aagaaacctg
     1021 taaatagcat caccacacca gtgactaaca tgtaactagt aaataacagg gtcgtttcag
     1081 ctggcgtagc gtttaactgt gcagcaatcg tttttaagat tgggtctact agacccactc
     1141 ccataaaagc gatcatacag gtagctgcta caactagcgc tgaacttttc ccctttgttt
     1201 cttcatttgt ttgttcttgt atatctattt gattctccaa tatttccatc cctttctctc
     1261 tattagcatc atacatgatt tattttacat gtaaatatgc acatgtaaaa cgtggaaact
     1321 ttactgtgat ataataaaga aaataagacg agaggtagga tttttatgaa tactttagcc
     1381 tatatattat tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt gaagcagctg
     1441 atcacaattt tttgggaagc ccatcacagt cagatctata caacgcttgc caagttagac
     1501 cagcagggtt atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa gatctaccac
     1561 ttgactgatg aaggacgtgc tcttgtagag gaatggatca aagaggaaac tcccgctcct
     1621 acgcaaaaag atgaatttct agcaaaaatc tatgcttttt cgacgctcga taaaacaaca
     1681 gctactggac tgctattcac tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa
     1741 aaaaaattga atgacctctc gctagacaag aaacaagaat ttggacgtta tgttgtgatc
     1801 cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa
     1861 atgcagcagt tttttgagta agggataaac agaacctttc tagaaatatc tattgaagag
     1921 caaattatct tcaataaaag agcaatacag ttggaaaaga aaaagcgatc cgaacgacag
     1981 gccaaaacct atcgttcgga tcactatttt tctagaactg cattcaagag actatgacaa
     2041 aaagcatgcc aaggcctttt ttcaaataac agtgttccat cattcattac tttgaattgc
     2101 ttttagcaca cttcttgaaa gcgggccaac gatgatcaac tgcaacggta aagcggcaat
     2161 cacattcatt ttccaagcat tcatataagc agagaataat tgatcaggta ttccttgttc
     2221 catgatcaca ccgaataaag acatacatgt caccattcct aaaaccatca agcatgaaat
     2281 agttaagatc atttggacct tgctttctgg atttactggt agtttaaagg caattttttt
     2341 cgccagcaca ccgatgatga agacatccaa aatgaaagcc accacgaatc caggcactag
     2401 accaatcaac aaacttgata aatgaagatc ttgatgcagc cataaattat acgcgctcat
     2461 ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt attccttctt ttttatttgt
     2521 aggcaatctg taccactcct ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat
     2581 ttctcgtaag tacttttttt attttctgcc cttttatttc ataaacttca tcagccgtac
     2641 gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt tcccggatat atttgcatta
     2701 gcttctctaa agcttcgatc gttgccatat caataaaatt agtcggttca tctaacagca
     2761 acacattact tggcttagta aaaagcaaag caatcgtcag acgggtcgct tctcccccac
     2821 ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac
     2881 tacggacaag cgattctgaa aactctgttt gtctcatgac aaaacgaatg atggattctt
     2941 cagatgtcat ttgataaccc ttttgctgat atacttgaaa gcttactttc ggagagagaa
     3001 ggattccttc tccttgtttg cggatatgtt ctaataaggt tgtcttacct gaaccatttt
     3061 caccgacaat cgcgattttt ttacctaaag aaaactgaaa atcaccattt accagtaatg
     3121 ttctcccact tctttccaat tggacatttt gtcccatgat gggatagcgg ctgtggagtt
     3181 caagagattt tggtactgga aaacggattt gtttcatctg ctgcggcttg gttgtttctc
     3241 ctattttttg gagtcttctc tctaatgttt tcgcattctt ttggatggct ttttgtacac
     3301 tgtctttttg tttagaggaa gacaaccgat ccggtctgat ttgttgcttt ttctttttac
     3361 tggacacttt ttccaacttt tccgcttgtt ttttcttttc ttggatcgat ttttccaatc
     3421 gttttttctc tttcatgatc gactctgctt ctcgtagctg agtctttttc tccaattcct
     3481 tttgagtgag ataggcacta taattccccg aaaactctcg gatttctccg tcttttactt
     3541 cccagatttt cgatgctagc tcatctagaa aatagcgatc atgactaaca aaaaccagcg
     3601 taccataata ataacgaagc tcttcaatca gtcgatcaat actttttcta tctagatggg
     3661 ttgttggctc gtctagaagc atcccttgtt cataagaaga aagaagacgt atcaactcga
     3721 tctttcgttc ttcccctccg ctccatttcc gctctgaagt ttcttccagt cttaactgga
     3781 aatgactggc taattccaaa tttaggtcct ttgcttcagc gagataggtt aattgaggca
     3841 ggtagttgaa ggtgatctct gtttggattt tcccatgatc gggaaaaagt tcaccggcta
     3901 ttagtttgag caaagtcgat ttaccgacac cattttctcc tatgatccct atacgatctt
     3961 gctgatagac acgaagagaa tcaatagata gttctagctg gttgccaaag ctgacttgta
     4021 gatttgttat atttactgct aaattttcca tatgttttac actcctattt catgtaaaac
     4081 aacaattatc gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat
     4141 cttttcctac tctgaacgaa ataactatga ctaaatcacc aaatctgtca tagctttcac
     4201 ctattctact gtcgttcgaa aaaatggaca tactccatcc atgaaattgt tgtttataca
     4261 tgtcttttga atttatttag ttgttattca aaagttcgaa acgtaatttc atcgatgcag
     4321 tcatttttcc gacctccttt tttattaggt taccacacac gattcaaaaa aagcaatagc
     4381 tactgaatag taactgaaaa ccttttattc gaacgtagtg atcttttctg gtgctaaacg
     4441 aacagactcg taaccttctt ctaccgcttt tcctattgat aaaataataa ctggaacata
     4501 acggtcttta tccaaaccaa atgcctctgc caactgatct gcttcgaatc caccaattgg
     4561 atttgtttca tagccatgat cacgcgcaac taacatgaat tgcatagccg ctaagctaga
     4621 atcgactttg actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat
     4681 cgcagctaat tgttgatctc tgacttcttt tggcatcttc cctgattcgt aagcttggtt
     4741 atagatttct tcgccatatt cgtaacattg catatcgcca aaaatcatga ccattgcaga
     4801 agaagtatca ttttgtctcg tattgaaacg gatcaaagga cgcaagattt cttttgcttc
     4861 ctcgctttca gcgaccacaa agcgccatgg ttgcatattg acagaagaag gtgcagtcgt
     4921 cgcttctttg atcatttcca gcatttcttc gtgtgggatt ttgtatgttt catcatatac
     4981 gcgaacggat cttctaccag tcataattgt tgaaaaatca tttttttgtt ccattattat
     5041 taacctcttt cgtttctgtt gttatattgc aaagttgggt attcaattta tctaaaagtg
     5101 ttagaaattg tttcatttct acatcgctga aagccgggga tagcacggaa cctgtatctt
     5161 tatggtccat ttcacaatgc tcgattttct tctgggcttt ttctgttagc gctacataca
     5221 cttcccggtt gttctgagga tttctctgac gcgaaacata gccatttgct tccaatacct
     5281 tcagatgacg agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta
     5341 gcaatggatt ttcttctttc agtctcatca tgatctcgta tctagtaaga cttataccga
     5401 tattttcttc aaagatctgg acgatttttt gatcggctaa tttgatttga tataaaaggt
     5461 gactaatctc ttctaaagac ataatttcct cctttctaat aattgactcg tcaacgattg
     5521 acttgtcaat aatagaataa aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa
     5581 aacgctctca atagaaaagt atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat
     5641 gataaagaaa gcacaacgct aaaaccaaga ttattgattc ttatcaaact atagaagata
     5701 gctcttctgc agtagaaaat tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga
     5761 atcccgtcga atattagaaa ttggatcagt atgcttaaac agtaataaaa aagttatgac
     5821 aagatgttgt cacaaccttt tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat
     5881 aagttcaacc aaacaaaaaa catgaaaatc tgtttttgtt tggctgttct tactacttga
     5941 tgcagggcag ctttttcact acctcttttt aataaccata aaatcctatt ccttcatatc
     6001 tccagcccat cggtgttagc caatctcttt ccttagcact caaggtatag tgatgggtac
     6061 cagttttttc atttggatta aatagtcgat aaattggctt ggaaccacca gaataaaaac
     6121 ttagaccttc ataacgccaa ccgatttttg ttaaagagtt gatctcagat tgtgccattg
     6181 tgtaatgatg gtctccactg taaggattgt ataaacggta gacaggttgt ccgctgtttg
     6241 gtgcttgcca agcaatccct tcatattccc agcctacagt tggcagataa tttttttctc
     6301 cggcattcaa tgtatataaa tgttctcctg tatgacggtt atacagacga tacatcggag
     6361 aagttgaagt tggtgctgta tcatactgtg tcagattata ggtttcgatt acattgttca
     6421 atgtagaacc ataattaggc gcagtggcat aacgacctgt tagccaagcc gtagcatctt
     6481 tgtaagagtt cgtattgctt ttccatgcac cggaatagta gtaaacgcct ggctgaaaag
     6541 aagtcgtctt caataccgtt acgttatctt gcaatgattc tttgtacgaa ggatattttc
     6601 taaaagcggc attcaccgta atccattgcc cattcacata ttccgaagta ggcatcgtaa
     6661 cagattggcc attatagctt cctttaatcc caaatagatt gtaattaggg gcagaagcca
     6721 aagtactgtt cccccagcca ctttcaacga tggcttgagc aatcatgact gatgcatata
     6781 agtcattggc acttgccaaa ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt
     6841 gagaattagc agatgagcga gcataaaaag catagccagt atagttttcg aattcagaca
     6901 aatctgcttg atgggtatgt gtcaatgttt gcccatctgt caattggttc gtgatttctt
     6961 ctgcattctc tggattgttc tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt
     7021 ctgttgtttc agttgttggt tcttcggttg aaattgtatt ttctgagaca gtagctgact
     7081 gcaaagtact ctctgtatcg ttttcaattc ctgtttctga agaactcgat gtctgagttg
     7141 cgacagtttc tgtcgattct tcagctagca ctgttgtgtt ggcaactgct agatttccta
     7201 aaagaataac gctaaataat aaattctttg ctttcatctt ttttctcctt tgaattatga
     7261 aacttttcct aaataacagt gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa
     7321 ataacatttg tttctttttg ataaatttgc tttttctaca taaaaagacg taaatgaaga
     7381 atattcttct ccattcacgc cttaaattga tggtcgtttg aataactatt taacaagtaa
     7441 ttctctttgg atattatccg ttatggatta tacttcctgt gtcgtcggac ggataatcat
     7501 gttactgaca gacatccggt ctggcgtttc tatcgcaaat gctacagcag aagctatatc
     7561 ttcggaggtc aatccccatt ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc
     7621 gctgatggtt tgatataatt cagtttgaac agctcctgga gagatgatcg tagatttgat
     7681 gttattttgc cgttgctctt gacgtaatcc ttccatgatt gcacggacag caaattttgt
     7741 tccgcaataa acggcggaat caggataaac aacatgtccc gccacagaat cagttgaaat
     7801 gatatgccca gatttttgtt tttccatgat tggaagtacg gcggaaattc catttaacac
     7861 acccatgatg ttgatatcaa gcatattttg ccattcatca cggtgacctt caactaatgg
     7921 agcggttggc atgattcctg cattattgta caatacatcg attcgaccga acttttccat
     7981 cgctaaatcg ataactgctt ggacttgtgc aaaatctctt acatctgctg tttgaatata
     8041 tagctcagct tctggtaaag attcttttat tgcttttagg cgatcttctc ggcgtgcagc
     8101 tataactagt ttagcccctt tttctgctaa tagcttcgtt gttgcttcac cgattccgct
     8161 tgaagcgccc atgatgacaa tcactttttc tgataaatca ttcattctat tctcctcctg
     8221 ttttgaaaaa tctcttgctt atactcacta tctcttattt catcttactg ttcaaataat
     8281 ccgttcgaca ggtttgacga ttttggcagg gattcctgca acgatggtat tgtctgggac
     8341 atctttcgta acagttgcat tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg
     8401 cagaatagtt gctcctgctc cgatccaagc atttttcttg atgtggattg gctttactga
     8461 caagcctctt cgttcttttg gatcggttag atggttcacc gtgatgaggt tgacacgagg
     8521 accaatcaat actgaatctt ctaaacagat gccacctaag tcaacgaaca tcacttgacg
     8581 attgataaag acatttttcc ctatactaat atgtttgccg aaatctgtat aaaaaggcag
     8641 cagaacagtc accgtatcat ctactttgtc actaatgata ttttctagcc gtttgcggat
     8701 atctgctggt tcatggtacc cagtattcag cgctgctaaa ggctcttcgt tttcagcttg
     8761 gatctgatgg atcacttcaa agagcggatc cgttactcga atctctttat tgacgatttg
     8821 tttttcgatg aggcttgtca ttttgctccc tccttttatc cttactttat aacggacggc
     8881 ttatcatgtc taatacttat aaaacatact taactattcc ttttaactat acttagtggt
     8941 atgctttttt aaaaacagga gggaaaaaat ggatattcgt atactgacgt attttttagc
     9001 agtagctaga gaaaaaaata tcagccgagc agcaaaatct ctgcatatca cgcaacctac
     9061 tctctcaaaa caactgaaag atttagaaga agaacttggt gtgacacttt tcactcgggg
     9121 ctcccgagag atacagttga cggattctgg tcgatatctt tattcaaaag gaaaagaaat
     9181 cttgtcgttg gtagaaaaaa cggcgaacaa tttaacaaat gacgcaatca tctctggaga
     9241 aatctacatt ggcggaggcg aaactaaagc aatctctttc atttctgata gccttcattc
     9301 gttgatcaag gcgcatcctg atatccagtt tcatttatat agcgggaatg cagatgatat
     9361 catagacaaa ctggatagcg gattacttga ttttggactc gtaatcgaac cgacaaacaa
     9421 acaaaaatac cagtcatttc gactgccagc ccaagatcgt tggggacttt tgatacatcg
     9481 aaatcatcct ttagcaaagc aaaccgtcat ccatccgcag gaactgaaaa ctgttccttt
     9541 gatcatttcc cggcaaacgt cagtggacag tcaactagct gaatggctag gtttcagcat
     9601 agatcagtta tcgatcgtcg gaacatataa cttattgtac aatgcttctt tgatggtcga
     9661 taacgggact tttggtgcat tagctctaga tgggatcatc aatacttatg ggacggaaaa
     9721 aatattcatc ccactcgatc cgccattaca agcttcactg aatctcattt ggaaaaaaga
     9781 ccagcctctt tctcctgctg ctcgtaaatt tttagatgtc ctaacggaga aatacagtta
     9841 agtagagttt ttttccttcg ctttcagctt tttaacaacg aataagataa gcaaaagatc
     9901 caacgattaa ataacataag tttcttaatt tatttcttcc ttttatttct gataattcca
     9961 gtaaattact ataattataa aaaaagaatt gacaaaacat aactgtctat ataaaattga
    10021 ttacgttaac aataattcgt ttcatgtgac tagatcatac taggcatgaa aaatctctga
    10081 ggaacatggt ttccctagga ggtttttcgt gtctattttt gtttggccgc aatcaactgg
    10141 tctagcaaac gtgaaaacca aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc
    10201 tttaatcaaa atgcagtatt ggtagatgac ggtggacagg aaaaagtagc tattggaaaa
    10261 gggattggat ttgacagaaa aagaaatgat ttgatttttg atcgtgatgt cgaacggata
    10321 tttgtcatgg aaccagaagg tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa
    10381 cgctttttat ttgcagcaga aacgatcatt gatcatgcgg aaactgtttt gatggaaaag
    10441 ttgaatgaac atgtattgat cgctttgacc gaccacattg ctttttctgc ggagaacatt
    10501 aataacggta tcgtcatccg caacaaactt ttaagagaaa tcgaagtgct ttacagtgat
    10561 gaattcagta ttgctcaatg ggcagtagag tatctgacaa aagagcttgg gattccatat
    10621 acttatgacg aagcaggata tatcgcaatc catattcaca gcggacgaag cggtcggaac
    10681 aataaccatc gcagtatccg tgaagtgacg attgtatctg acatcatcca tttagtggaa
    10741 tcagaattgg aaacagatat ccattcagaa ccattttcac tgaattattc aagactggtg
    10801 aatcatttac gactgcttct acaacgaacc catgcgcaac aatatgctgt tttagacacg
    10861 gaaatcgtag aaatggtcaa acgaaaatac ccagaaagct ataagatttc taaaaaaatc
    10921 cgtgtgttgc tgaccaaaga ataccagctc gcgatcacga aagaagaact gggttacttg
    10981 gccatccata tcgagcgttt aagaagcgca atcgtacaaa caaatgttaa caatcatgag
    11041 gaggaaaaaa attgaaagca tatctacaac gaatgggacg ttcccttcaa cttccagttg
    11101 cggtcttacc tgctgctgct ttactagtag ggatcggaaa ctggtgggca tcttatagta
    11161 acgacattgt cgctcatttt ttacaagctg gcggtaacgc agttttaggt caattacctt
    11221 tgctgtttgc cgttgggtta gcactaggga tgtcagtgga caaatcaggt gctgctgcct
    11281 tggcaggttt ggtcgcattt gaagttccag taaatgtttt aaaaactgat tcagtagcta
    11341 ctttattgaa tatcaaacca gaatcagttg atccatcttt tgctcagatc agtaacgtat
    11401 ttattggtat tattgccgga ttgattgcgg cagctcttta taatcgtttc cacgaagcaa
    11461 aattaccgat ggctttatct ttctttagcg gcaagcgtct agtgccaatc ttggctgcct
    11521 ttgtcatgtt gctgatcagt gcagtgctac tgctcgtatg gcctccggtt tactctgctt
    11581 tagtttcttt tgctaaattc attttgagtt taggctgggg tggtgcaggt ctttatggct
    11641 tcttcaaccg tttactgatc cctacaggat tgcatcaagc aatgaactcc gtgttctggt
    11701 ttgacgtagc aggaatcaat gatattggta aatttttggc ttctgaagga acaaaaggcg
    11761 taacaggaat gtatcaagct ggatttttcc caatcatgat gttcgggctt ccagcaggag
    11821 catatgctat ttacagaaat gcacgtccag aaaacaaagc aaaaacagct tccttgatgc
    11881 tagcagcagc ttttgcttca ttctttactg gtgtgacaga accacttgaa ttttcattta
    11941 tgttcgttgc ttggccatta tatgtcttgc atgcaatttt cactgggatt tctttagcag
    12001 tgagtgcatt cttccattgg acagcaggat ttgcctttag tgccggattt gtcgattact
    12061 tcctatcttt gaaaaatccg attgcgaatc agccattgat gttgatcgtc caaggattag
    12121 taacagcagt gatttactac tttggcttta actttgctat caaaaaattc catctgatga
    12181 ctcctggccg cgaggaagca gatctttcag atgacgatac tgcaacgact aattcagaca
    12241 ataaatatgc ggcacaagca agcaaaatct acgctgcatt aggcggagcc gacaatgtaa
    12301 cttcgatcga taactgtact acacgccttc gtttgcaagt caaagataca ggcctgatcg
    12361 atcaaaataa aatcaaagcc actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg
    12421 cgcaagtcat cgtaggtaca gaagtacaat tcgttgcaga tgagatggca aaactacatg
    12481 gtggtgctgc agcacgacct gcacaaacaa acaccgtggt taaaacagaa acatttgccg
    12541 gtgaaacgat cgaacaagat atttacgcta tcgcaaatgg gaaactgatt ccaatcactg
    12601 aagtttcaga tgatgtattc gcggaaaaaa tgatgggtga cggctatgct gtattaccag
    12661 aaaatggaga aatcttctct ccaatcgctg gtacgatcac aaacattttc ccaacgaaac
    12721 atgctgtcgg aatccaaaca gatgctggta tcgaagtctt gcttcatatg ggcatcaaca
    12781 ctgttgactt aaaaggcgaa ccattcactt tatatgtaga agaaggtcaa aaagtcgcaa
    12841 gaggtcaatt gattgcttta gttgatcttg ccgctattca atctgcaggc aaaaacacgg
    12901 atatggtcgt tgtattcaca aatggcgaca aagtccaaag cttagaaatc gaacccgcac
    12961 gtgatgtaaa agcaaatgat aaaatcggct ctgtttccaa caaagcctga gccatcgtct
    13021 tccccagact tttttatcga taaacaaaca ggagctgaga caaaaatctt gtctcagctc
    13081 ctttttccga ataaaaacgg cgttctcctg ccaactccgc ggtaataagt ccagctaaac
    13141 gaaacatatg aagagctatt ttttatttga ctcttcttat tacttgatgc agaacagctt
    13201 tttcacaaaa tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca
    13261 ttattcctga tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa
    13321 tactttttct aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat
    13381 cgctaaaaac atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa
    13441 cgctgcatca cttgcttcaa taatttctaa ctctttacct gtcgcacgct ctaatgcctt
    13501 ggctaattct tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact
    13561 ttctactagt acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt
    13621 tccatttcct gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa
    13681 ttcattctct acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat
    13741 cattttttca gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc
    13801 aaaacttgta taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt
    13861 atgttcggct tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt
    13921 caacgcttct ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc
    13981 ttctttcagt gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg
    14041 aaccatcttt tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac
    14101 taaataatgc atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca
    14161 tttataaatt acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga
    14221 aaggatgaca actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct
    14281 atattgaaat gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg
    14341 aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct
    14401 ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac
    14461 tagatgtcta tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa
    14521 atccaaattg cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc
    14581 tgcagaaaaa ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa
    14641 tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat
    14701 aattctctct actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt
    14761 ctccggggga caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg
    14821 ctgaagtcat gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg
    14881 aaaaatgcaa aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc
    14941 gctactttca cgacctctat attatcatgg tttattgact accggtatcc agatttctcc
    15001 catatatttc ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc
    15061 gtaatcttct acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa
    15121 ttcttcaggc gattctgcta aagcgcttac aacaagatac gttccttttg ggaatgggat
    15181 gattcgcgta gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt
    15241 cccgttatag ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc
    15301 atctattgtt ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc
    15361 gacaaaatcc gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg
    15421 tttttcttcg atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc
    15481 ttacataaaa ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt
    15541 ttttaactat ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc
    15601 ctctccccat tttgtaaaag agatctcggg agcttcgact aacccataat ctgaagaagg
    15661 aagccattct gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt
    15721 attaggaaaa atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg
    15781 attttctttt gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg
    15841 gtctccatca aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt
    15901 atgcatctct tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt
    15961 gactccttca aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt
    16021 cttttcgata cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg
    16081 tggaaaagat ttttgtatgc cgtatctact gatttctgaa ggcagatatc ctgaccaatc
    16141 acgaaaagct cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt
    16201 cactcttgat ccatctagaa gatcgcgatt agccaaagcc aatctacggt ttttgatata
    16261 gtccattagc gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat
    16321 tcctgtattt tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta
    16381 atcgatcaat tggttcagat ctttaagcat atttatcttc ctccttgatt gcctttttat
    16441 catattatca aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga
    16501 aaatgttgta tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat
    16561 tatctgccta tctatgtttt acctttagaa aaataaattt tggggaagac agattctaag
    16621 taaacaaagc ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta
    16681 caaagcaaat tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata
    16741 catcatacct cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt
    16801 tgtcagataa ataaaaaaca aaaaaagttc catctttaaa acacgctatt cttttacttt
    16861 ttctcacatt ctctaagaaa agaaaaggag aatgaatttt tgacaattcg agcaaaagtg
    16921 attatcaaat atgtaaaatc agctactttt ttcttaaacg tttctttata ctaaatataa
    16981 ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt tcctgaaaac
    17041 gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt gaactataca
    17101 agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt gatcacttgg
    17161 gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa
    17221 cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc caactactca
    17281 ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg ggagctttta
    17341 acagagattc ttatagaaca tccgcacttt atcgaacgct tcaaagttaa gatgccattt
    17401 catgcaataa attattcaga taaattaaca actatcatcc ataaatacga tcatattatc
    17461 aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa
    17521 gaagcagttg aatcgcttat tcagcaaaat gcctatcttc ccgcattcga attgatcaat
    17581 gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg aacccttctt
    17641 ctgatagagg actgttatta tttatggaaa gaattattag cagaagcacc tcatgcagaa
    17701 aaaaggcaaa tgttctcttg gttcgtctgc caagtcgatc atactgacgc ctcttattca
    17761 aaacgctact ctataaaaat cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa
    17821 aagaaaaaaa tagataaaaa actaaagaaa cggtaaagaa agacgatttc aatgaaaaat
    17881 agcaagtaaa cgataacaac catcctaaat ccacaagaaa tacagcagac cttgctttaa
    17941 aaacacgaat catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca atattagtca
    18001 tttgactatt aggtacgcgt tttgttttat aagtcatgac aaatttcctt cgttgctgga
    18061 tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt
    18121 tccgctgcat ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac
    18181 cctaacccta gtgcagcaat cattgttgcg cctaatgcag gtccttcctc cacacttaaa
    18241 caatagaccg ttgcatcgaa aatatcagct tggatttgta gccatgcaag atttttcgcg
    18301 cctcctccca ccgaaacaat acgtgtaaat ctgcgacccg ctagttcttt acgtttcaat
    18361 cccaccattg acaaatgctc attaaaaatt tagaatattc acaagtttaa cgacaattat
    18421 gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact
    18481 gcgatcaaag acacgttacc taccgttttc ggttatatcg gtattggact tgcatttggt
    18541 atcgttggga aagctgccgg atttcatcca ctagtcgtca cgttgatgtc cctgctggtc
    18601 tatgctggtt ctgcccaatt tatcacagtc agcatgcttg ctagtcacag cccattgctt
    18661 tccatcgttt tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt
    18721 gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca
    18781 gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt
    18841 cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt
    18901 ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc
    18961 gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa
    19021 cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt
    19081 atcccaagca acttagtgat tgtagttgta acactgattg ggtgcggatt gggggtgtgg
    19141 atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac
    19201 ttggctttct agggtcctac catttgtctt actgaaaaaa ttcgatttac cgcaaccgtt
    19261 gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct
    19321 atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt
    19381 tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggcaggaat
    19441 tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta tggcaaatta
    19501 ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg tattcttgct
    19561 tatccgttga acggaccact aacaacgact tttcctaaca tatgtcctgt ttctacgtct
    19621 ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt gctcaatgta
    19681 ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt
    19741 tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa gctgatagat
    19801 tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt tcccacatgt
    19861 ccaaatggtc ggacaaggtg cttgatttga tcaaagtagc gggtaatatc gaataataca
    19921 gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa gtcgtgatga
    19981 taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt cggacttgca
    20041 gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga accgacgccc
    20101 ccggatccgt tgatcactaa gatttttttc ccgcgattgg cattttcttc tggagttaga
    20161 ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa ggctgcagct
    20221 tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac tagttggtat
    20281 tcttgattgc ttcctgctct tgttgttgta cctgcgtaaa aaacgcgatc gccgacagaa
    20341 aatttttgca cttcgtcacc tacagcaaca actttcccga ctccatcaaa acctaagata
    20401 cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg attaacagat
    20461 actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg ttctttttca
    20521 tcaataaagc tttttggatc atcgatcggc aacccttcat aaaaaccgac taccttcatt
    20581 aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc atccatacag
    20641 tgttcatttc aatatatata gtatgctatt tgttccacct tttttattca tcataattat
    20701 tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac
    20761 ttttgtcaca acctcttata aacggtgttc tcctaccaac tcttcggtca caagtcaaaa
    20821 tattcatcaa acatga
//
LOCUS       DACEOM010000056        20497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEOM010000056 DACEOM010000000
VERSION     DACEOM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.057"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..270
                     /locus_tag="IW781_001996"
     CDS             <1..270
                     /locus_tag="IW781_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1182990.1"
                     /translation="PKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFC
                     VPDSFSNYRISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(728..1003)
                     /locus_tag="IW781_001997"
     CDS             complement(728..1003)
                     /locus_tag="IW781_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182991.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1457..1663
                     /locus_tag="IW781_001998"
     CDS             1457..1663
                     /locus_tag="IW781_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182992.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1859..2026
                     /locus_tag="IW781_001999"
     CDS             1859..2026
                     /locus_tag="IW781_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1182993.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            2052..2396
                     /locus_tag="IW781_002000"
     CDS             2052..2396
                     /locus_tag="IW781_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1182994.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2401..2682
                     /locus_tag="IW781_002001"
     CDS             2401..2682
                     /locus_tag="IW781_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1182995.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2785..3099
                     /locus_tag="IW781_002002"
     CDS             2785..3099
                     /locus_tag="IW781_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1182996.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3077..4771
                     /locus_tag="IW781_002003"
     CDS             3077..4771
                     /locus_tag="IW781_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1182997.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4791..5969
                     /locus_tag="IW781_002004"
     CDS             4791..5969
                     /locus_tag="IW781_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1182998.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5932..6618
                     /locus_tag="IW781_002005"
     CDS             5932..6618
                     /locus_tag="IW781_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1182999.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6618..7778
                     /locus_tag="IW781_002006"
     CDS             6618..7778
                     /locus_tag="IW781_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1183000.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7788..8663
                     /locus_tag="IW781_002007"
     CDS             7788..8663
                     /locus_tag="IW781_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183001.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8660..8971
                     /locus_tag="IW781_002008"
     CDS             8660..8971
                     /locus_tag="IW781_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183002.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8961..9314
                     /locus_tag="IW781_002009"
     CDS             8961..9314
                     /locus_tag="IW781_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1183003.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9304..9705
                     /locus_tag="IW781_002010"
     CDS             9304..9705
                     /locus_tag="IW781_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183004.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9698..10102
                     /locus_tag="IW781_002011"
     CDS             9698..10102
                     /locus_tag="IW781_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183005.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            10114..10722
                     /locus_tag="IW781_002012"
     CDS             10114..10722
                     /locus_tag="IW781_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1183006.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10742..11104
                     /locus_tag="IW781_002013"
     CDS             10742..11104
                     /locus_tag="IW781_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183007.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11107..11289
                     /locus_tag="IW781_002014"
     CDS             11107..11289
                     /locus_tag="IW781_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183008.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11306..14737
                     /locus_tag="IW781_002015"
     CDS             11306..14737
                     /locus_tag="IW781_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1183009.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14788..15525
                     /locus_tag="IW781_002016"
     CDS             14788..15525
                     /locus_tag="IW781_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1183010.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            15535..17826
                     /locus_tag="IW781_002017"
     CDS             15535..17826
                     /locus_tag="IW781_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183011.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            17850..19988
                     /locus_tag="IW781_002018"
     CDS             17850..19988
                     /locus_tag="IW781_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183012.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIEFGGYDYSGNPNFLSKLSYDLVV
                     NQNGSVGTVSQGENSFKYNKTTKDVDGSVALYYKAGGRANWLPSNKRIVMTVKLRAGV
                     GYIPADGRKVLIRYRYIDNGTSKIPVDLQVNSASLTQEWQEFTVTGTTQTFSQQANDP
                     WVQFYVQMGILGEIEMSYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENIANKTIKY
                     PIKTSAYATYTASNIENYQANQTYTLTMKATKPATQTFGVYIKGGTLGVGNMVPVEGK
                     TDEWSLSFTVTQAHINAGVTRDLSIYQLPQATIGACQIDWLKIEKGDTRTPNIEQYKY
                     RGIGMRDSNNPKDYVWDLAPEYVEDNLATDVKISEITGKANNYTDGKVSEINSQLTAS
                     INEVDTTAKDAQTKANANETAIKSTNSRVNEIENIITALTKEETYLLPASIVSGGSLK
                     FTSRGGMTVATGSFKLAKDIAFGEVLVNDLPDSMINIDNNAIGTGAVTGNSVSQIYVE
                     SNTRKVVANTAMKKGQWFTVGFSYHSKLSI"
     gene            20045..20287
                     /locus_tag="IW781_002019"
     CDS             20045..20287
                     /locus_tag="IW781_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1183013.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYESLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            20303..>20497
                     /locus_tag="IW781_002020"
     CDS             20303..>20497
                     /locus_tag="IW781_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1183014.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
BASE COUNT         7192 a         3050 c         4318 g         5937 t
ORIGIN      
        1 ccgaagcatg gcaataaggc agaagacgcg attattcaga tgatggatat agaaacggag
       61 agagatgcga ttctagcggc tttgatggct cttagtctga ttagtcgtca gatactctac
      121 tacagcttct gtgtgccaga tagcttctca aactacagaa ttagccgtga agtgggttat
      181 tcagaaagaa gtatacaacg gatgaagtcg gaagctctaa tagagtttgc agaagcatat
      241 aaacacggaa aaataattgc ttataaataa tttggcggtt ttttggcgga atgatggcgg
      301 tttttagaca tttaccagtg ttattatggt agtgtcgaaa gattaggaaa caggatcgac
      361 aaaataaacg aagggaggca atctccctca tcgttttaaa cttcttctta gatagacagc
      421 aactaattat gaagaaaagg atgtgaattc aactcctcct aaattcttca tcggttgctg
      481 tttattttga ttagacagca gcactcacaa aactaaacat gttcttgtcg tctttcaact
      541 gctgctgtct attaatttat gtattggagg gaagaaaaat gaaattatca gtagaaggca
      601 ctccagaaga aatacaagaa ttgctccaag ctattggtag tagcaaggag caaaaggtaa
      661 gtgtttctaa ttcagatatt gacggtattt gcaattgttt aaacagagaa aaaatatcta
      721 atccttacta agagaaaata tgttcgttaa tgaatttcca attttcctct gtagctctac
      781 cttgataatt tttttgcagt tcacagatga ataaggaatc gttattatca agtactgtat
      841 ttaatttttc acacatttga tttggattca aattagtttt aactaaccaa aaagattttt
      901 gtagctttat atatgcccca aattctttta ttatttcaaa tactttatca tacttttgcc
      961 ctgggttatt aagatcataa ctaatcatgt agggtttata catgttgtct tgcacatcct
     1021 tgttttatat ttcagcggac cactcgctga taaataaaat tatacgctta gtatttattt
     1081 tcacaataag aatttatcgt aataactttt gagtaaatag aacaaaaaac ctgcactagt
     1141 ttccgctagt acaggcagtg actatatcgg ttgataatct agcatatatt aaaaataatt
     1201 gcaagaaaag aaatttatag tggtttatac caaattatca aagcaatgtt attttgttgc
     1261 tgtctattgt tttttaatta ttcacacgat aactaaaggt gggtgaagag aaatgattcc
     1321 attaataatt tcaatttttg cgctctgtct taatgtctat atgattggat ttaaaaatgg
     1381 gcaaaataaa aaatagtagc agccaagaat aattttatag tgtcactgtg gcggaaaggg
     1441 tagacgctaa gcatgtgtgc taggtcaatg cttcggcaac catgcaaggt tcgattcctt
     1501 gccagcgaca ttaaatgcct atgacggtta cgactaccga aaaaagatcg ttaagaagct
     1561 atacggtgct acgtacggca atgtagtaag tgtgctatct gtacaccacc aagcttcggt
     1621 cactgtggcg gaagtagaag acgcagcggt aaatggcgag tagcctcgtg agagcctggt
     1681 aagttctcgt gagtggtgca atcccactcc agcgacttta agcaaccgag gcatcggcgg
     1741 tttaaaaata taggggtgcg caatttcgta cgcgttttgt gcatcgtgca agttactatt
     1801 acatattaga tcactctttg agtggtcttt ttatttttgc acaaaggagg aaacaacaat
     1861 gtatagacca caatacttag aacagaagta tgaagtaatc actgttcata aaggtaatgg
     1921 cgaaaaagta tatgagtata gaagaccaat aaagagcgat acatataaac gaaaggaaaa
     1981 caatgaagtt attccattgt atggcaaaag aatagctaag cattaaataa gattgcgaaa
     2041 ggagacggaa catgaccgag gaattctata gatggctatt acagttgaca agagaagatc
     2101 gtttggttaa gttctatcag tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac
     2161 gagatcacta tgaatgccaa gagtgtagaa gactaggtaa gtatcataga gtagagaacg
     2221 ttcatcatat aaaggaagtc aaggatagac ctgacttagc tttagattta gataatctta
     2281 tttgtttatg tgttgaacat cataatgaag ttcatggcag atatcttaca gcattagata
     2341 aacaagagaa gaagatagaa agctttgcta acttcgatgc aagtgaaagg tggtaagtgc
     2401 atgatcatca atgataatgg cagagagtat gatacagaaa agattgaaga gtattcatct
     2461 tatactcagg gattaattaa acgtttgata tacgttcgct atgtaggtat tagggatctg
     2521 ttatcagata actgttgtag taaatacaaa gtgaatcaag taagagaagc gttgaataaa
     2581 gataataacg tcgaaagaat aaaaaatgtt tttggatata gtattgaaga gattaattat
     2641 tacattgact tcgctgaagc tttcattccg atggtgagat aaccccccct taaaataaat
     2701 cgcaaatttt ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa
     2761 ctttatcatg aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga
     2821 gaaaaattac gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca
     2881 ttcttagata atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa
     2941 gatatatccg agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg
     3001 cttgtttctg tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa
     3061 ttggtaggtg aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa
     3121 agctatagaa gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct
     3181 agaagaaaga gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc
     3241 aatcaatata ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca
     3301 atgttttatt tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct
     3361 ttcattaatg ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct
     3421 actaaccgct aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag
     3481 ccaagcaaag actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga
     3541 taaaaaagta tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact
     3601 tcgttataac acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt
     3661 tgatgaaatt cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg
     3721 taaaattcga gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc
     3781 gcttgatgac attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa
     3841 ggatggagca gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga
     3901 agttgatgat cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcagattt
     3961 aaaacggaaa atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac
     4021 gttcatgacc aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata
     4081 tgatgatgtt ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg
     4141 aacagtcgat tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga
     4201 taaagatgtt tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa
     4261 catcaaacga gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg
     4321 aaaaaatata gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta
     4381 tattaaaaaa atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga
     4441 agcaggtttt actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga
     4501 tctggttgat gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg
     4561 ttggtattgc atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa
     4621 aatagaacct gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt
     4681 tttagatgat atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac
     4741 aggaactgga tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt
     4801 ttcaaaaggc ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa gaatactttt
     4861 gtaaattgca agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga
     4921 ttgcaaatgc gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga
     4981 atgatcttta ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta
     5041 gaaaaaaact gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta
     5101 ataattctag cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag
     5161 atgatgtgtt ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa
     5221 gtgatgttat ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt
     5281 atcaagcgta tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc
     5341 gtagatacgt actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa
     5401 tcaataaaat gatgacatca caatttaagg cttttatgga agctgataat gcaggtgcgg
     5461 tatttcaatt acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt
     5521 caagagatat aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg
     5581 ttccgaaaaa cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt
     5641 taatgttcga aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct
     5701 atgaagtaga agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga
     5761 ttactagctt caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta
     5821 caatcaacga aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg
     5881 aaatatttat aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca
     5941 atgaaaacat ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga
     6001 tttattggtt ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg
     6061 gatagtctag gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta
     6121 tttcaaggga ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg
     6181 attaacggct tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa
     6241 atctacaaca atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc
     6301 gatgacttcc gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca
     6361 tataaaacac gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg
     6421 tttatggatg cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa
     6481 aatagctcag gtactgaagc taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt
     6541 aaagaaaaaa gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga
     6601 caaggagatg taaaataatg acagttaaaa atttaaaagg tgtaacagct gcaagcgacc
     6661 aattgatgaa agcttttaaa gatggtaacg aagaatcttt tagcgcagct atggtaagct
     6721 tatctaagga aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat
     6781 tagtattaat gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg
     6841 aagtagtgaa aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg
     6901 aacgtgtatt tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta
     6961 acacaactgg tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta
     7021 aactttgcga agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc
     7081 agttcaagct atcaggttat attcctgtat gtaaggcaat gcttgattta ggtccagtat
     7141 ggttagatcg ttatgtccgt actgttttag tagaatcatt gagaattgca ttagaacaag
     7201 caattgttga tggtactggt aaagatatgc cagtcggaat gatgcgtgac atgagcaaac
     7261 aaactagcgg agaatatgct gaaaaaaaag cagaacctat tacagcttta gatgctgtaa
     7321 ctatgggcgg tttgatggcg cgactatcaa aattcaatat cgaaggtgtg aatgatccga
     7381 tttatcgtaa tgtgaatcct tctgatgtgg tcctaattgt gaatccaact gattactggt
     7441 ctaaagtatt cccagctaag actgtactaa ctgctaatgg agaatacgta caagtattgc
     7501 cagtaccagt ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca gttattgggg
     7561 tagcctcaga ttacttcatg ggtgtaggat ctacactaaa aattgaagct tcagatgaat
     7621 accattttgt tgaagacgaa cgcatttatc tagctaaaca atatgcaaac ggtcaaccta
     7681 aacgtaacga tagtttcatt gtattagata ttagcgcttt gggaactact actacaacta
     7741 caaaaccaac aaccacaaca actacaacac aagcgtaggt gatcagaatg aagtatattc
     7801 tttgtcagcc ggcaatcaat cggtttaaat gggagcttga agtttgttta actaatctga
     7861 agaaactagg aatcaaagat atcgtattgc ttttcagcag acacgatgat cagattccta
     7921 ttttttttga gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca
     7981 aagagtatat tccttcgatt aaaccatatt tatggtggaa atatttagaa gaagatcatt
     8041 cgcgtgagga cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta
     8101 atttgcgcaa attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc
     8161 taagtcttga ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa
     8221 acattgtaaa tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac
     8281 agtgggttat taaccgtcct aaagcgaatt attggaaaaa ggtttatctg gattctaatc
     8341 ggctatatcg ctaccttaga gggcaaaaaa caaatataca aatctggaca gccgagatgt
     8401 gggcacagct ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag
     8461 acttttgttt tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg
     8521 ctggagtaac aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc
     8581 cttttgatga agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag
     8641 caattaaggc ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt
     8701 gaaggataat atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc
     8761 taaaaatgct gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag
     8821 cttagaatac ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga
     8881 gtttgaacaa aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga
     8941 atatattgca ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg
     9001 gttggttaaa gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg
     9061 tagaagatac agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata
     9121 taacagctat ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc
     9181 caatcgccaa gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca
     9241 acgttatcta tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg
     9301 aatatggtga ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc
     9361 aaaacttcct tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat
     9421 gagctacctg atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact
     9481 gagccaaaat atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa
     9541 gatttaacag gagactcact agatattatt tcattgattc aaaatcgttc gattcgtttt
     9601 cagagaatgg atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg
     9661 gtatttacgt ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct
     9721 aaaaataaat ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga
     9781 acgactgatt aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac
     9841 agaaaaaatt cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag
     9901 tcgtccactt aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt
     9961 tgagtattta aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga
    10021 atttatgaga agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca
    10081 attcgataaa ttaaataaat agggggaaca acaatggcta aaacaaaaac tgtagtaaca
    10141 acgttcgata acgtaagtat caaacgaatt gcttttaatt ttaagaacgc aggaaatgca
    10201 atcgcaacga attgtaatgg acaattagat ggcgaaacag aaatgcaaac ggtggttaaa
    10261 aaatgtggag cgacagaagt aaaatcaaaa tctaaaccaa tcaatatgac ggtaacaatt
    10321 actgcacatg taccgatgga agtttatcga cgtttcaatg ggttgaaaca agatggacgt
    10381 attaaaccag gcatttactc ttacggtcct gattccgtag gcgaagattt ctcacttgct
    10441 gcagagatcg tggatgactt cgaagaaaat agaaagttag ttggtatgtt agcatgcact
    10501 tcgaatacag gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa
    10561 ctagaaacaa aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca
    10621 gaacttgaag aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa
    10681 aaaacttcag ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga
    10741 aatgaacgaa gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc
    10801 taaactgaat atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc
    10861 aaaaatgact gtgggaaaag gatccatgga tatttctgtt attcaaggtg caaaggctgt
    10921 gtatattgct taccgccaag cgaacatgac tgattatatt tcattcgatg aatttatcga
    10981 taaatgggat tttgatatgg ctactgccag ctatatttat caattgatga tgttcaaaca
    11041 agcacgcgat gcttatcaaa aagaatttga aaaagcaaat aaggaaaaaa agcttcaaaa
    11101 gtaaaaattc caaagctctt agttgaaacg tgggtcgatg tctattcgat gttgaccgac
    11161 gttttttcta tgccttcaga tttggtttta agcgatatct gtttagatga cattttgcaa
    11221 atggcttaca acaagagtgc ttatgaagga tggaaaaact atgcaataaa ccaatcccag
    11281 aaaaactaaa gaaaggaggt aaaaaatggc taaaaagaga acagaagcag aagtaacttt
    11341 catagctaac gatgacggat tgaaatctac gttaaaagaa atcagtgctg aattaactaa
    11401 aaatagagca gaattaaaac tagaacaagc tcaattacaa cagactggtt ctgaatcaga
    11461 caagttagga agtaaattat cttctttaga aaagcagtat gaattacaaa gtcaaaaagt
    11521 tgaagtaact agccaacgtt tagccaatgc gaaaaaatat tatggagaaa attccaccga
    11581 agttcagaaa cttgaaagag aactgattaa tcaacaaaca gcgcaacaac gtttgtcaaa
    11641 cgaaattgat aaaacgagta atgcactagc tcaagcaaaa ggcgaaatac agacgtacga
    11701 gtctacaatg caacagttgg atagcgaaca aaaaaatgtt caagctagtg cttctctgat
    11761 tgaatccgaa tacaaaaaat ggcaagcaac tgctggtcaa tcagcttctg aagccgagaa
    11821 attagcgaaa gcccaagaat atgtttctca acaatctgaa aatgcggaga aaacgataga
    11881 tatcctaaga cgacagttag aagctacaca gtctgaattt ggcgctacat ccacagaagc
    11941 aatgcagatg gaagcgaagc ttaatgatgc tgaacgtgaa tttgaagagt taggacaagc
    12001 tgctaaaaat gtagatacaa ctaacttgga cgatatcgga agcaaaatag atatgaataa
    12061 tttaatggaa gcttctgacg ttttaagcga cattggcgat aagcttacag aattagggaa
    12121 acaagcagtg gactctgcta acagtgtagg tagttcccag agtaagatac aagctaattt
    12181 tggtttgtct aaacaagagg ctgaagaatt aacgaatgta gccagagaca tttattataa
    12241 aggttttgga gaatcgttag atcagtccac agatgcattg attttggtaa agcgtaattt
    12301 aggagattta aataatcaag atttacaaaa tatcacggaa caagctatgg tcctagaaaa
    12361 caccatgggc gctgatatgg atgaaacgtt acgtggtgta aatggcttaa tggtcaattt
    12421 cggcttgagt gctcaagatg caatggattt aatggtttcg ggtactcaaa acggtttaga
    12481 taaaacgcac gaattaggcg acaatatggc agaatatagc caattatgga gtcaaatggg
    12541 atattcagct gatgaaacgt tcggaatgct tcaaaatggt ttagatgcgg gtgcttataa
    12601 ccttgataaa gtcaatgact tagttaagga aatgggaata tcgttaacag atggtcgatt
    12661 tgagcaaaac atggatatgt ttagtgaaag tactagaaaa gcttttgaag agtggaaaaa
    12721 tggcggagga acacaaaaag acgttattaa ttccatgatt caagatttta gcaatatgga
    12781 tggtcaatac gaccaattaa ataaagcttc gacaatttgg tctgcgcttg gcgaagataa
    12841 tgcgatgaaa gttgtccaat ctttaactga tgttaaccat acatttgatg atgttagtgg
    12901 atctgcacaa aaaatgaatg aagattctac tactccgttg caagagttga acgggaaaat
    12961 agctgaatta aaggattcat tagctcctat aggcaacaca atcatagatg cactcgaacc
    13021 agtaattgat tttctaggaa agatggctga tgcgtttaat aatcttccac aaccagtaca
    13081 ggatttcatt gttgctatag gtggtcttac agcagcattt ggcatactgt taccggcaat
    13141 actagccgtg tcatatttat ttggtccgat gatgcttata attggaggaa ttatagctgt
    13201 tatagcaggc gttattgtgg caattaagaa ctggggtgca attactgact ggtttagtgg
    13261 tttatggaaa aaatttactg attggttggg tgatacttgg gaaagtataa aagacggagc
    13321 ctcatcagtt tgggatgggg ttaaagaaac ctggtctgga tttgtagatt gggttcaaga
    13381 tatttggcaa ggagtttctg attggtttgg agagctatgg agcggattag ttgaaggagc
    13441 ttccaacatc tggcaaggag tccaagaaac ttggcaaaca ttcgttgatt gggtttcaaa
    13501 tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg taggaattgt
    13561 tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata ttatcgtggg
    13621 tatttttgat gtagctggac agttattggg cgcagcttgg caagctgttt ggacacctat
    13681 ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagta tcgcttggaa
    13741 tgggattgta actacattcc atactatatt tgatccagta gtggcatggt ggaatggtat
    13801 atggacagct attagtacta cggcttcaaa tatttggaat tcaattagtg caacagcttc
    13861 tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag ctgctacagt
    13921 aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag
    13981 ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg ctataaacgc
    14041 agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatta gcggaatttg
    14101 gaacggtatc aaaaatactg ctttgaatct ttggaatgga attacaagca ctattagctc
    14161 taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt
    14221 ttccgacata ttcaataatg ttaaaagtac aattgctaac atatgggaag gcatcaaaaa
    14281 gactgttagc gctccaattg attggattag agacaaaatc agtggcattt ttgataattt
    14341 gaatatttct ataccacata ttccgttacc ggaatttatc atggaaggca gctttaaccc
    14401 gctaaaaggt caaatccccc atttgcgtgt aaaatggcat gctaaaggag gtatctttac
    14461 taaaccaact ttactaggtg gaatgaacgg tgtcggtgaa gcaggacctg aagcagtttt
    14521 acctttgaaa agatctgttt tgcaagaaat tggtgatcgt atcttgagta gcacatcagt
    14581 ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga
    14641 tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac
    14701 aaaaattcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt
    14761 ttttagtatt aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga
    14821 tttcctgatt atttgcataa attggcttta gctgaaagac caaccatacc ttctccaaaa
    14881 agacagagag ttgaaacttc tgttttaggt aggttaggtg gcttagtaca agattactcg
    14941 tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa
    15001 gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa
    15061 ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg ggatgcagaa
    15121 aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga
    15181 gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat
    15241 agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc
    15301 atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga tgtagtcatt
    15361 gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc
    15421 aacaaaatga agaccatgca atatccgaca ttgcaagtgg atatcaatca ttttaattgc
    15481 actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa atagatgatc
    15541 gataatttaa taactattta cgataaaaat gacgcgaata atttagctga acatttatat
    15601 gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta
    15661 aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt
    15721 gtagacggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg
    15781 atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt
    15841 ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaagatcact
    15901 gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt
    15961 tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta
    16021 gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa
    16081 gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt
    16141 tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga
    16201 gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa
    16261 gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt
    16321 aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca
    16381 aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat
    16441 caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat
    16501 gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat
    16561 gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa
    16621 aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaacggt
    16681 ggcgaaatca gtgatttact caatgatatt gtagatcatc aaactgatat gattactggt
    16741 caaaatggtg gttatgtttt attagatcct aaagaagcgc ctagtcgtat tttgattatg
    16801 gacacaccag ataagaatac cgcaaggaat gttttacaaa tcaacaatgc tggtattggt
    16861 ttttctaaaa ctggcattaa tggaacatat gaaacggcat ggacgttaga tggcggattc
    16921 aatgcctcgt ttattacggc tggtgaaata gtaggaatta ctattagagg tactacatta
    16981 attagtgatg gtgctgatta tagaacaagt attgctaatg gcaaaatgac ttggtactca
    17041 aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc
    17101 ggtattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt
    17161 aacttggttt ttagtacgtg ggataatggt aataacagac cgtttctatc ttttggtgcg
    17221 cccaatttca ggtatagcaa tgctagttat gtaacttctg gcgacggtag ttctttaagc
    17281 attaatggta gtgcgggtaa ctcatgggaa tttaaggtag ctggtaggac tatgaaattt
    17341 actagtgatg gtatgctaac gttaccaggt tgtttttttg gttcatggga agatgggaaa
    17401 cttgctaggt ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact
    17461 aaaaactcaa ctgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact
    17521 ccagaatatt atttcgctga ttatggggaa gccgttacag gtgacaatgg taaagttcgt
    17581 gttgatattg accccatgtt tgctgaaaca gtaaatctaa gtcggtatat gacacatgtg
    17641 acacctacag aactagtttt gtgtgctgtt actcatgaag atattgacca tttcatcatt
    17701 gaaactagta agccaaacgt attagttaga tggaatttag tggcacaccg tctagggtat
    17761 gaagatgtta gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt
    17821 ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag
    17881 atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga
    17941 tcacggtgac tgttaaaccg tttttaaatg gcttgcagat ggatacgagc ggcggaacat
    18001 ttactttaaa aggaactacg ccatctaacc gttacgtaga tagtgtcgct actagtgtaa
    18061 ctagtgaaga agtcacattt tctcttgatg gcacatttat gagtgaagca gggtattata
    18121 aacactgcta tgtagaatat agaaaagacg atcaaatttt aacgacgcaa gatatcattt
    18181 ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatat gtttcgcaat
    18241 tagaagaatt gattcgaaag tataacgaaa cttttgatgc ttttctggct gaaattaaag
    18301 gtagaatgga tagcttaaat aaacagatta ctgatttaac tggtcaagct aaaacactac
    18361 aagacaagtt agatgctctg aaagaagaaa tttctaaatt aggtaactta catgtgatgt
    18421 actccaacag catcgaattc gggggctatg attattcggg gaatccgaat tttctaagta
    18481 agctatcata tgacttggtg gtaaatcaaa atggttccgt tggaacagtt tctcagggtg
    18541 aaaactcgtt taaatataat aagacaacta aggatgtaga cggttcagtg gctttatact
    18601 ataaggcagg aggtagagct aactggctac catccaataa aagaatagta atgaccgtta
    18661 agcttagagc tggagttgga tatattccag cggatgggag aaaagtactg attagatata
    18721 ggtatattga caatggaact agtaagatac ctgtagactt acaggttaac agtgcttcac
    18781 taactcagga gtggcaagag tttactgtta ctggaactac tcaaacgttt agccaacaag
    18841 caaacgatcc ttgggtacaa ttttatgttc agatggggat acttggggaa atagaaatga
    18901 gctatgacat caaaatcgaa gaaggctcaa cagccacccc gtaccagcct aacttacttg
    18961 atgcgccgta ttatttgagt aaggtggctt tgggtgagaa tattgcaaat aaaactatta
    19021 aatatcctat taaaacaagt gcttatgcaa catatacagc ttccaatata gaaaattatc
    19081 aggctaacca aacttacaca ttaactatga aagctactaa accagctaca caaacttttg
    19141 gtgtttatat aaaaggtgga actcttggtg ttggtaatat ggtaccagtt gaaggtaaaa
    19201 cagacgaatg gtcattatct tttacagtta cacaagccca tattaacgca ggggtaactc
    19261 gtgatttaag tatttatcaa ttgccacaag ccacaattgg cgcatgccaa attgactggc
    19321 ttaaaattga aaaaggcgac acccgaaccc caaatattga gcaatataaa taccgaggaa
    19381 tcggtatgcg agactcaaat aatccaaaag actatgtctg ggatctagca ccagaatatg
    19441 tcgaagacaa tcttgctaca gatgttaaaa tttctgaaat tactggtaaa gcaaacaatt
    19501 ataccgatgg gaaagtatcg gagattaatt cgcagttgac tgcttctatt aatgaagtag
    19561 ataccacagc taaggatgct caaacaaaag cgaacgctaa tgagactgct ataaagagta
    19621 cgaatagtag ggttaatgag atagaaaaca ttattacggc cctaacaaaa gaagaaacat
    19681 atttgctacc tgctagtata gtaagtggcg gatctcttaa attcacaagt cgtggtggaa
    19741 tgacagtagc tacaggatca tttaagttgg ctaaggacat agcattcgga gaagttttag
    19801 ttaatgattt gccagattca atgattaata ttgataacaa tgctataggg actggagctg
    19861 tcacaggtaa ttctgtatcg caaatatatg ttgaatctaa tacgagaaag gtggttgcga
    19921 ataccgctat gaaaaaaggt caatggttta cagttggatt ttcgtatcac agtaaattat
    19981 ctatatagat ttagcgtact cattcgagta cgtttttatt ttgcaatgaa aggaggctag
    20041 ttggttgaaa gatgaagcaa tacaagacgt ggtagaacgt ttagtgcgta ttgaaacgaa
    20101 gttggataat tacgaatcat tacgcgaaaa agcggaaagt gcaaaagata gagcggatca
    20161 ggcatattct attgcgctta ataatgcgga agatatcaaa gaaatgaaag ccaataataa
    20221 atggtcgtgg ggttacatga tcggtttagg cattacgatc attggctatt tcttgactaa
    20281 attgtaaagg aggtgagaag aaatgatttt accagataag tattatcaag tcattaaatg
    20341 gacagtttta acagtattgc cagctgcttc tgtgttagta gccacgttag gaaaagcgta
    20401 tggatggaat ggaacagata tgacagtact cactatcaat gcagtagcaa catttttagg
    20461 cgttatcact ggtgtgtcgg cttataattt gaaaaaa
//
LOCUS       DACEOM010000057        19494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEOM010000057 DACEOM010000000
VERSION     DACEOM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.058"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            66..845
                     /locus_tag="IW781_002021"
     CDS             66..845
                     /locus_tag="IW781_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183015.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            891..1835
                     /locus_tag="IW781_002022"
     CDS             891..1835
                     /locus_tag="IW781_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1183016.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1851..2630
                     /locus_tag="IW781_002023"
     CDS             1851..2630
                     /locus_tag="IW781_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1183017.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2642..3340
                     /locus_tag="IW781_002024"
     CDS             2642..3340
                     /locus_tag="IW781_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1183018.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3368..4132
                     /locus_tag="IW781_002025"
     CDS             3368..4132
                     /locus_tag="IW781_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1183019.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4176..5627
                     /locus_tag="IW781_002026"
     CDS             4176..5627
                     /locus_tag="IW781_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1183020.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5659..6795
                     /locus_tag="IW781_002027"
     CDS             5659..6795
                     /locus_tag="IW781_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1183021.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6795..7901
                     /locus_tag="IW781_002028"
     CDS             6795..7901
                     /locus_tag="IW781_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ1183022.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            7907..8929
                     /locus_tag="IW781_002029"
     CDS             7907..8929
                     /locus_tag="IW781_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1183023.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            8951..10006
                     /locus_tag="IW781_002030"
     CDS             8951..10006
                     /locus_tag="IW781_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1183024.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10624..11859
                     /locus_tag="IW781_002031"
     CDS             10624..11859
                     /locus_tag="IW781_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183025.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            11943..13031
                     /locus_tag="IW781_002032"
     CDS             11943..13031
                     /locus_tag="IW781_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183026.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            13028..14449
                     /locus_tag="IW781_002033"
     CDS             13028..14449
                     /locus_tag="IW781_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1183027.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            14542..15201
                     /locus_tag="IW781_002034"
     CDS             14542..15201
                     /locus_tag="IW781_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ1183028.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            15220..16803
                     /locus_tag="IW781_002035"
     CDS             15220..16803
                     /locus_tag="IW781_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAQ1183029.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            16938..17516
                     /locus_tag="IW781_002036"
     CDS             16938..17516
                     /locus_tag="IW781_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183030.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            17462..17980
                     /locus_tag="IW781_002037"
     CDS             17462..17980
                     /locus_tag="IW781_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183031.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            17988..19028
                     /locus_tag="IW781_002038"
     CDS             17988..19028
                     /locus_tag="IW781_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ1183032.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            19277..>19494
                     /locus_tag="IW781_002039"
     CDS             19277..>19494
                     /locus_tag="IW781_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1183033.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALEYYQQYKKNKSNCGRRPLVLP"
BASE COUNT         7111 a         2478 c         3825 g         6080 t
ORIGIN      
        1 ctgtcatttt tatgtaagtg aattatatct taatttatct atcatcaata cttaggagtg
       61 aatctatgga aaagccattc gtcaagtcat tgactgctgt ggcggtaggt gcaggtgcta
      121 ttgcgatctg tttgtttgga taccatatca ataatcagcg gcaacatcag caacggatca
      181 attatgcaga atctgccatc accaatcaaa aagacacctt gacttcttta tcaaaagaag
      241 tagacaagct gtatagtaca aaagaaaaaa tttttctaaa ccctgaaata acagaagaaa
      301 caatcacaag tttgtcaaat aagctatcct ctgtcaaact ttcagcagat gattacgaca
      361 tcaaagaatc agaattgcct aaagaagctg ttgcaatcca agatgagaag aaagcaatcg
      421 tgacacagtt agatagtgca gaatcaaaac tgaagatcca gacagctgtc aacgatttat
      481 ttactaaaaa tgtatcaaac tggcagcaag ctgtagacga tgtcatcatc aaagaaaagg
      541 tgacaagtgc agatgtcgct cgtgtacgtg aaaatatgag cttttttaaa gatagtcctt
      601 ggaaaacggt tgttatgcaa tatctaggat tcgctgatat tcagatcgcg caagtcacgc
      661 aactagatca gttattcgat acgatgttaa aggatggtca agtgactgcc accgctactt
      721 acgatcaata tttgacggtg ctcagtcaaa tagagcagat ccggaatgaa aaaataagcg
      781 cagcatacgc aacaaaagca caaactgttg ctgagcaaat gggctatagc aatataagct
      841 attgacagtt aaaacgttga ttcaagcaag gttagcaaac ggaggaaact atggcaagac
      901 gaagaagaaa atcaagaggg aaaaaaatat ttctgattat tttagcagtt cttatcgtat
      961 tagtattagc aattgtagga gtaggagcaa aattatatct ggatctctca cattctattg
     1021 agaaaactta cgaatcagtg gaacgaaccc aaggagaacg aacgaaacca gttgatctgg
     1081 acaaacaaga atctttctct gttctattga tggggattga tactggagat ttaggtcggg
     1141 tcgatcaagg aagatcagat acgatgatgg ttgccacagt cagtcctgaa gacaaacaga
     1201 caacgatcgt cagcattcct cgtgatacgt atgtcgacat cgtaggtcgt gacacaaaag
     1261 acaagatcaa ccacgcttac gcattcggcg gagctgccat ggcgatggat accgtacaaa
     1321 aatatgtaga catcccaatc gatcactatg tctctatcaa tatgaaaggg ctaaaggaac
     1381 tcgttgatgc agttggtggt atcgaagtca ataatgacct cactttctca caagatggct
     1441 atgactttcc aatcggtaaa attacattga acggggatca ggcattatct tattctcgta
     1501 tgcgttatga agacccaaat ggtgattacg gtagacaagg gcgccaaaga aagatcgtag
     1561 aaggcgtagc gaaaaaagta ttgagtctaa acggtgtcag caactatcaa tccgttttga
     1621 atgctctaga gtcgaatatg aaaacagaca tgaactttga tgaaatgaaa aaaattgctt
     1681 tagattatcg ggaggcattt ggtacgatta agcaagatca gctgcaagga gaaggcttca
     1741 tgcaagataa tatctcctat caaaaagtca gtgatcaaga acttgatcgt gtgaaaaaag
     1801 aattaaaaga tcaaatgaat ctagagaata aataggaaag tggagaaaaa atggaagaga
     1861 caatcagctt gaaagaaatt tttgaaacat tacgcaaaca cttggtaata atcatcatct
     1921 caatgtttgc tggcttggcc atctcaggaa tcgtaacatt tttcatcttg acaccaaaat
     1981 acagttcaca agcacaattg atcgtgacac tgccacaatc agacactgca aatgtcaatg
     2041 atgtcaatac gaacctgcaa atgatcaata cgtataaaga tatgatcgtc agtgatttag
     2101 tgctcaatga agtcaaagat cgtttagaaa cagaagacaa tgtgaaaatg actgctgaac
     2161 aaatcaaaga tgccatctca gttaatcagg cagaaaattc acaaatgttc tcgattcagg
     2221 caataagtac gaatgcagtg gctgctcaac agatcgctaa cacaacttca caagttttcc
     2281 aagagaacgc aaaagatgtg atgaatgttg ataaaatctc gatcatttca ggtgcagttg
     2341 ccaatacgac acctgtttcg ccaaataata aattgaattt aatgactggc ttaatgttag
     2401 gaatgatggt aggtgtaggt ctggcattct tattggaatt actcgatcgg acagtgaaag
     2461 atgaaaaata tgtgacagat acattaggat tcccaatctt aggaacagtt ccagaaatgt
     2521 cagcgaaaga attgaatgct ggtatccgta aaaaaggacc agctcagaca caaccaaaag
     2581 ctacaaacga taaaacagcg ccatcacgca gaagccgctc aagagtctag gaggaaagaa
     2641 aatggcacga acacagaaac aaaaagcagc ccaaactcat gcagtcagtt tggtcacact
     2701 caccaatccc tcttcaccaa tcgctgaaca atatcgtacg attcggacga atatccagtt
     2761 tgcctccgca gctggtcagc aaatcaaaac gattgttgtg acttcatcag gacctggaga
     2821 agggaaatcg accactgcag caaacattgc cgttgttttt gccaaatctg gtcaacgggt
     2881 gctgctagtc gatgcagact tacgtaagcc cgtcatctat aaaacgttca agttgaacaa
     2941 cgcaagcgga ttaagtactg ttcttagttc atcaggaagt gtagcagatg agatccaacg
     3001 aacaccagtc gacaatttgt cgattctgcc aagtggacct aaaccaccaa atccatcaga
     3061 actactaagt tcgccaagaa tggatcagat tttagcagaa gcaagacaac tatttgatgt
     3121 agtgatcttc gacatgccgc cagtcgttgc cgtaacagat gcacagatca tgtcttcaaa
     3181 aacggatggc accttattgg tcgtacggga aaatacgtca cgaaaagaat cattgactaa
     3241 agcaaaagaa ctgttagata tggtacaagc acgagtctta ggtgtcgtat acaacggagc
     3301 agaacacagc aaagatgctg gttattacta cggaaattaa ggaataagaa gagaggagac
     3361 aagagaaatg atcgatctac attgccatat cctaccagga atcgatgacg gggcagaaaa
     3421 cttagaagca agtattgcaa tggctgaaaa agcgattcag caaggcatta cgcatatttt
     3481 atgcacacca caccacaata atggaaaata cagcaatgaa aaatcacaag tcatttctct
     3541 agtcgcttcg ttacaagccg aattagagaa aagacagttg cctttgacgt tattggaagg
     3601 acaagaagta cgtattaccg gaacgctcat cgaagacatc caccgagatg aaattttatt
     3661 tacagattta gatgatacat atcttctgat cgaatttccg acactggaag tgccgttata
     3721 tgcagaacgg cttttttttg agttgttaga actaggaaag ataccagtga tcgtccatcc
     3781 agaacgcaac gcgcattttc gagaagaccc aaatcatttg cttccgttcc tagatatggg
     3841 atgtttggcc caacttactg cgccaagtta tgttggtgta tttggcaaag atatccagaa
     3901 aacagccaaa acaatggtca aacataatct cgtgcagatg gctgcttctg atgcacacgg
     3961 ggtctctaag cgcacctttt acttaaaaga gtgctatgag cagattgcca aggagtttgg
     4021 taaggaaaaa gtagtacaga tgaaacaagt cgcaaaggat ctgatcaacg gggatgagat
     4081 caattatcca atctatgaag aggtaaaaaa gaagaagttt ggtttatttt agttctttat
     4141 tcgtagataa aaaggaaatg aggagagagt gagagatgta tagaaaaaac tcaagtggtt
     4201 ggtctaaaca tattgatttt attttattag acttattttg tgtccagctt gctttttata
     4261 tatcatatgt aatgcgacag ggtgactgga atccgtatgt gattcctatt tatcgccaca
     4321 tggcaatttt tatagaactt gagaatctta cgattatatt tttatttgag gcatataaaa
     4381 atgttttaaa aagaggatat tataaagagg ctgtagcttc tataaagcaa tcttttatgc
     4441 tgttacttgt gtgcagttta taccttatta caatgcagga tggaaatgat tattccagag
     4501 tttctttatt tataatgggg gtggtgtatg gattattaac atatataatt cgtatacttt
     4561 ggaaagggtt gttgcatcac cgcatgaaaa acggtgaaga tgtatcgtta gttattgtaa
     4621 catctcaaaa gattgcaaaa aatgtagttc gtaatcttaa agagaagaat tatggcatgt
     4681 ataaacttag tggtgtgatt gtagtagaca gagatatgta tggaactacc atcggaggtg
     4741 tcccagtagt tgcaaatgct gataatgcag cggaatatct tttacagaat tgggtagatg
     4801 aagtattcat caatgttgac gaaagtgttc catatcccaa agaattaatc gccagatgtt
     4861 cggaaatggg acttgctgta catatgaatc ttacaaaagt gtctgatagt acacgtggaa
     4921 aacaatttat tggccgagtt ggtgattata ctgttattac atctagtatt aatgttatga
     4981 cgatgaggca agcatttata aagagaacga ttgacattct tgctggaatt attggatgta
     5041 ttgcaacagg tgttattttt atcttcgtag caccagttat ttacatcaaa tcaccagggc
     5101 cgattttctt tgcacaagaa cgtattggtc aaaatggtaa gacatttaag atgtataagt
     5161 tccgtagtat gtatatggat gcagaagaac gcaaagcaga gttgatgaaa gaaaataaga
     5221 tgagtaataa tctcatgttt aagatggatt ttgacccaag aatcattggc aataaaatac
     5281 ttcctgatgg aacaaagaag acaggaattg gacaatttat tcgatctaca tcattggatg
     5341 agtttccaca attttataat gtcctaatgg gaaaaatgag cctggtgggg tttagacctt
     5401 gcttgaagtc agaatatgag aagtatgatt ttcatcatcg tgcgagaatt gcgatgaaac
     5461 ctgggattac tggaatgtgg caggtgagtg gtagaagtga tatcacagac ttcgaagagg
     5521 ttgtaaaact tgatgcagaa tatatcagga attggagtat gggattagat tttagaattt
     5581 tgtttaggac agtcaaaact gtgcttcata gaaacggttc catgtaagat aaagatacta
     5641 tagaaggagt aaaacgtgat ggcaagaaaa aaattaaaaa tctgtatgct aggtcataaa
     5701 agaattccat caagagaagg tggaattgag atagtggtag aagaattggc aacgcgaatg
     5761 gttgcacttg ggcatgaagt gacttgctat aatcgaagtg gtcatcatgt tagcggaaag
     5821 gaatttgacc agcataaaaa taaagaatat aaaggtgtaa aattaaagac tatttttaca
     5881 ttaaatataa aaggaattgc agcaatgagt tcttcagtat ttggtggaat tagagcagca
     5941 ttcggaaaat atgatgttgt tcattttcat gcagaaggac catgtgctat gttgtggttg
     6001 ccaaaacttt ttgggaaaag atgtgttgca acaattcatg gtctagatca tcaaagagaa
     6061 aaatggaaca agcttgctag tacatacatc atgttagggg aaaagtgtgc tgtaaaattt
     6121 gcagatgaaa taattgtact tagtgaaagc gtccaaaatt attttgaaga tatatatgga
     6181 agaaaaacaa ggtttatacc aaatggcgtt aaaaaaattg aaataaaatc agcaggattg
     6241 attacagaaa agtatggatt aacaaaagat tcttatattt tgtttttggg gcgattggtt
     6301 cccgaaaaag gaattcggta tctaattgag gcatttaaag atgttcagac agataaaaaa
     6361 cttataatag ccggaggaag ctctgataca gatgaatttg caaatgaact aaaggaatta
     6421 gcaaaaggtg atgagcgaat tatatttact ggatttgtac aaggacagga attagaagaa
     6481 ttatatagta atgcgtatat ctatacactt ccaagtgatt tagaaggaat gccattaagt
     6541 ttgttagaag caatgagtta tgggaactgt tgtatagttt caaatatctc agagtgtacg
     6601 gaagtagtag aagacaaggc tatgattttt aagaaatcgg atgtaagtga tttaaagata
     6661 agattagaag aggcatgcaa tcaatcggaa atggttaaag ttttgaagaa tcaagctact
     6721 gagtttattt gtagtaaata taattgggat aaaatcgtac aagaaacatt aaatttgtat
     6781 aggggaaata tataatgatt cgaatattac agtgtgtgaa tgatatgcat cgtgcagggt
     6841 tggagacgat gctaatgaac tattatagga atatagatcg aactaaaatt cagtttgatt
     6901 ttttgacaca tcgtccatat aaaagtgatt atgatgagga aataataagt cttggaggaa
     6961 aggtctatta tgcaccgaga ctttatccac aaaattatcc taaatatttt aaatggatgg
     7021 aacaattttt taaggatcat ccagaatatg agattgttca ttctcatatt gatgcgatga
     7081 gttatttacc tttaaaagct gcaaaaaaag ctgggattcc gatacggata gcacatagcc
     7141 ataatacatc gatagataga gattttaaat atcttttaaa gcaatattat cgatgtaagt
     7201 taacgtctgt tttgacacat gaattttcat gtggaaaagt agcaggtgat tttttgtttc
     7261 gaaatgataa attcactttg attccaaatg cagtggatgc taaaaaattt tattatgatg
     7321 aaagtattag aaatgatgta cgaatgaaat taggaatttc aagtcagacg tttgtaatgg
     7381 gacatattgg aagaatttca tattctaaaa atcatcgttt tttgattgaa atttttaaag
     7441 aatttcacgc tattaagaat aattccgtat tattaattat tggaactggt gatatggaag
     7501 aagaaataaa aaattatgca aagaaaagtg gcatagatga tgatattaaa tttttaggaa
     7561 atagaaatga tatagaaaaa ttttatcaag catttgacgt attaatgttg ccatcattat
     7621 ttgaaggggt tccgttagtg ggtattgaag ctcaatttgc agatttaccg tgtttctttt
     7681 cggagaaagt tcctacagaa gttgctttca gtgataagac caatttcata agtttaaatc
     7741 aatcggcaaa agaatgggcg agagagattg caaatgtaga tatctctcat cgagattcag
     7801 aacgttcgat tttaataaag gcagattata atattcaaac agcatacaaa atcttagaat
     7861 caaaatatta tgagttattt gaattaatac aaagaggtta aagaatatga cacagaaaca
     7921 ttcaagtata aaaagaatta tacatcgcat atccgaagaa ttgaatgcaa caagacgaat
     7981 tgcaaagata acatcttgga aggaagcatt tgccacgtta caagctaaaa ttgatattca
     8041 agtcatgaat cgtaatggat ataatgaaag taaaaaacag aaggaacatt tattaaaaaa
     8101 acatgaggta atgctgaagt attatgaaaa gactttttca gattttttga aaacatatga
     8161 ttatagtcaa aatcaaataa ttaatcaatc aaaatcacca tattctaact gcatttgggt
     8221 ttgttggtgg cagggattgg atcaatcgcc ggaaattgta aaagcttgtg taaaatcaat
     8281 aaaacgaaat gcagggaaac atactgttat aattttaaca gaagataatt atagacaata
     8341 tgttgatata ccaaaatggg tggaagaaaa gaaaaacaaa ggaatcatta cgagaacaaa
     8401 ttattctgat ttattaagat tatctttgct agcaaaacat ggaggtttgt ggttagacgc
     8461 cacattttat tgtacaaaac caataccaga tgagtatttt gatatcgata tttggtctat
     8521 taggaggcct gattatgctc acgcatccgt agcatcagga tattttgcgg gttattcatt
     8581 agcatgtaat caagagtcta gatggatgtt tgctataatt agagactttt ttttaaatta
     8641 ttggaaaaat aatgatatga tggtggatta tttgatgata gattatatga ttgttttagc
     8701 acagagaata aatcatgaaa tagcacagac atttgataaa atacctagta acaatccatt
     8761 atgtgacgaa ttgattaaaa atttaaataa tatatatgat gaaaaagtat ggaacttatt
     8821 aaagaaagat actttactgt ttaagttgac atggaaacaa tcttttaaaa agcaaaaaga
     8881 tgataaagtt actttttatg ggatgttggt agaaaacaaa ttattataaa tattagaggt
     8941 ataaaaagtt atgaaacaca aggtaagtgt gatagtacca atttacaatg ttgaaaaata
     9001 tttaagaaaa tgtgtggata gtttattgag tcagacatta aaagagatag aaatcgtatt
     9061 agttgatgat ggttcgccag atgcttcggg ggaaatagca gatgagtatc aaaaaaaata
     9121 ttctaatgta aaaacaatac atagagaaaa tggggggtta ggaccggcaa gaaatacagg
     9181 aattgagaat gcgacaggag aatatgtggc ttttcttgat tcagatgatt gggtagaaag
     9241 tgatatgtat gaaaaattat acttagttgc gtccaaagaa aatgctgata tagtagttag
     9301 tggacattgt gattttgcga atgacaaagc tatggtgaaa aagcaacatc ctttgagtgg
     9361 gaatgtatat gatacgccta atagtattaa aaatgtacga aaaaatttat ttggacatag
     9421 tccaaaagat caagaagtag aggccttccc gatgtcagta tgtatgtctt tgtataataa
     9481 aaatctgcta gataaatata gactaagatt tgaaaaaatt ttatctgaag atacaatttt
     9541 taacttgaat gcatatgatg tcgcaaataa aattgctttt acagaatata cagattattg
     9601 ttatagaaaa gaagaacaag agtctattac aaaaagttta tctccaaata agcagacgga
     9661 atttaaagaa tttttaataa ctctaaaagc tatagcatcg aaagaagatt tagaatgtca
     9721 aatcagagca aaacgtatgg caattgatta ttgtaggctt tatgtgggct tagttgatga
     9781 tgcaaatttg ggaattaaag agaaaagaaa gtatataaaa aaatttattg aagatgaggg
     9841 attaattcaa tgttggaagg gatatcctac aaatacgcta ccatttcaac aaaaattttt
     9901 tcataaaatg cttgaatgta agtgttatac aatattgttg ttgttaagta gaattagaaa
     9961 gataattaaa aatatgacca gaaaaaaatt gtttataata ttataaacta aaagtatatc
    10021 aaataataga aattaacaag ttacaaaaaa atattataaa gaataatatt ttctaaaaag
    10081 taaactatag taatgtggat tatcaccttc caacagtata taaaatgagc gtaaaaacgg
    10141 ctatttttgc cttaacaacg atttttttgt atacaaatac gatatgctca ttaattattg
    10201 gaatttgtag tgctgtttaa attaatgaag gtgtaattat atgagtgatc attttgaaaa
    10261 agtttgttga gaagatgtgc ttctagtcat gtgatgggag aaaataattt aactgttaga
    10321 gtgaatagtt aaattattct ggttatatga aaaaagtagt ttctaaaata atcttgcagg
    10381 gattaactta aagaaattga gagaatagtt tctttgaaat ttctatatac aatgagaagc
    10441 tgaaatttac ttttatttga tgactggaaa tagaaattaa atatattgta ttgttacatt
    10501 aaagaaaact tgtatggaaa aatcagtaca ataccattat ttaatgttct tcacttaaag
    10561 cgcaaaaaat atcagtttat taaactcgta tatagaacaa atatatgtag acggggcgaa
    10621 acgatgagtg taaaaaaaat gctatttatg gtaagtacaa taattgtatt aacatcccga
    10681 ttttggggaa ttaatactag tttatctatg cgatatttta ttatggcaat atgggattta
    10741 tattttatgt taagctattt ttcttatcgt gagaaaacat ataaaaaata cgttatcaag
    10801 aataactttt ggttgactat aatgccgttt gtgatatttt ctatctatac tctaataata
    10861 tggggaattg gttcaaatgc agaatttgaa aattttataa agttatgttc cacgttgctt
    10921 tatttaattc ttgcatatgg ttacgcatgt acagcatttt acttatttaa ggcagaagga
    10981 attgatttta ttttttggtc aggtgttatt tcatatatga gtggttcagt tttttattta
    11041 attgtatctt atggtttaaa tggtttaatt tcttatataa aaagtcttat aacaggtgaa
    11101 acaaatactg caaattttgc tatggaggta catgatttaa cttttgcgat gggggttttt
    11161 ttcctttatt acgtattttt tgaaaataaa aatacaaaaa aacataagat aaaaattatc
    11221 gtatctttaa ttttaatttt catgggattg aagagaattg aactgttagc tttaagtggt
    11281 gcaattctag tgtattatgt tttattaaag tggggaaaaa agattcaaat ccgtgcatta
    11341 gtgtgtacta tttgtgcaac tgttatttcg ttagtgttta tttacattat agatacagga
    11401 attttggcat tgcttatgtc aaatttgggc attacagatg acggtgcgag gctttcattt
    11461 tatctatatt catctaaatt ttatgaactt ggtttaggat atgtaggaac aggatggaca
    11521 tggttttcaa aatacttttt taatctttat ttatcgcact ttaggttaaa tggtggaaga
    11581 attgcagcaa gcctacacag tgatatatta actatttata tagaagttgg atgtatagtt
    11641 tttattatat ggatgttttg gttatttttt gtgaaaccga taagactaac taaaaaattt
    11701 ggattgaaaa caggggagtg tgtattatta ttaactatat atatgtttat attatatttg
    11761 acggataata cgctcactta tccagacact caaatgattt ttctcttagt acctatgata
    11821 gtttcgtggg ttgataatca acaacgaata tcggaataga tagctaaagt atattttaat
    11881 tatgaattta taattagttg taaaagttaa tatttaacgg acaggaagta gggtgaaaaa
    11941 atttgaaaaa aattttagtg ttagcaaata catattatca aataatagta gcaatacaaa
    12001 tgaaaaatac aattttttcc gatgagtatg ttgaaattat tatttctgat cattctaaaa
    12061 acactaaaga aattgcgaaa aaaatagaag aaaaaaaagt gtttgatggc tgtcattatt
    12121 tagaaagcaa atcttttgat gataatattg ggtattttca aaagattaag gactgtcttc
    12181 agatttcttg tggtaaatca aatcgatatg cctattattt gaaaaactgt aaaagtgaat
    12241 attttgatga atttatttgt ttttcaatgg gagaggtttg gttggaagga atatatgctt
    12301 gcttatatcg aaataataaa aatgtacagg tatcaatttt agaagaaggt attttaagct
    12361 atggagttga cattattcca acagcaagaa caaaagctat cgcagtactc aattacttaa
    12421 aaaaaaggac tccactatat aacaaaagct ataattttta ttgttttttt cctaatatat
    12481 atacaggaag agagaataca atacaaattc cattagttac agctgggtca agaaccgcat
    12541 tgcaactttc ggatattttt aaccctatga tagatattta taaagaaaaa aaatatatat
    12601 tttttacgtc tgtttatgat tttgagggcg gagaacctgt cggagaatat gaattagtat
    12661 gcaaaatagc agacttagta ggaaaagata atctaattgt taagacgcat cctagagatg
    12721 taagaacaat ttataaagat aatggattta tcgaggataa aaattcatca ataccttggg
    12781 aggcgattca attgtcaggc gattttaacg ataaaatatt tatgacaatt aattcaggaa
    12841 gtgttatttc aggtagtaca atgtctgcaa aacctgtacg cacgtattat atgtataaat
    12901 tgtgtaacat agaaggaaat ccgtcttgca aaaagaatgc gtatgatatt gagcgattgc
    12961 ttaagcaaaa aacgatgaaa aacattttaa agaatgtgaa aatagcagaa agcttagatg
    13021 atatactatg agcaaaacta aatatttaat taaaaatatg ggaatattaa caataagtaa
    13081 tttttcttca aaaattttag tttttttgtt agtcccttta tatacaagtg tattatccac
    13141 aacagaatat ggcacatatg atttgacaat ttcaacagcc acattattgt ttccaatttt
    13201 aaccttaaac attgtagatg ctgtgatgcg ctttttaatg gataaagata aaaataaaaa
    13261 aaatatagca tctatagcga ttaaagttat atttttaagc gtgatagtct ttggatccgt
    13321 aatgtatttg ttaaatatta caaaattatg gcaagatgtt tatggattag aaaaatatat
    13381 ttttttgtat tatatatcct atgtatggaa tcaatttcta atacaatttg ctaaagggtt
    13441 agaaaaagtt aaagctatgg gcatagcagg tattatcagc acaattgtaa caataattgc
    13501 caatattttc ttcttgctta ttctgaaatg gggattaatt ggttttttca ttgcaaatat
    13561 tttatcacag attatttcag ctacttatct gtcagtatta gtgagaatat gggaatatat
    13621 cgattttgag gctaaagatc ctcagctaaa gagagaaatg ctaaattact cgattccatt
    13681 aattgcaact gttgttggat ggtggattaa tagtacagct gataggtatg tggtttcctt
    13741 tatagtaggt gtctcggcga atggcttatt gtctgtggca tataaaatac ctcaaataat
    13801 taatactttg cagggaatat ttatacaggc ctggcaaatt tctgcaatta aagaatatgg
    13861 agataatgag actgcaaaat tttatggaaa tacatttgtt atgattaatt tgttaatgtg
    13921 cataacctgt tcaagtctta tcatattaac tagaccgttt tcacatattc tttatgcgaa
    13981 agacttttat gaagcgtgga aatatgttcc atttttatta atagctaatg ttttaaattg
    14041 tgcatcaggt ctgttaggac ctatcttatc agcaaaaaaa gattctaaat ccatgatgtg
    14101 gtctgcaatc ataggggcat tagttaatgt gattatgagt gtagtccttg tttataagat
    14161 aggggttcaa ggtgccacaa tagccactgt tatatgttca tatgtaatat atgttgttcg
    14221 taaacgagct gtgggaaaag atatttttat aaagcattat catattgttg tggaatcttg
    14281 gggattattg tttgttcagg caatcataga aatatatttt aaaaattatg taatagaaat
    14341 tataattatg attattttaa ttattataaa tatcagttat attaagcagt tgtgttatag
    14401 aggaagaaac attatttcaa tgattaagag tcgagagcac aaacggtaga cgatgttatt
    14461 tttataaaaa gatataattt attaatggtc gataattatg aaaggaaaaa tattcttaaa
    14521 aaaaagagta taggatgtcg tatgaaaata gtcgctatag taccaatgaa gttaaataac
    14581 aaaagattgc cacaaaaaaa cacgaaagct tttaccaatg gaaaaccatt gtgttattat
    14641 atactttcaa cacttttaaa agtaaaacag attgataaag tgtatgtcta ttgtagtaac
    14701 ccagatatta aagaatttat accagaaggt gtaacatttt taaagagatc tagttcctta
    14761 gatcaggata caacgagtat tacacaggta ttagccggtt tcacaaaaga agtagaggct
    14821 gatgtatatg taatgacaca cacaacagca ccatttatta gcgaaaaatc tattgaaaaa
    14881 ggactagatg ccgtgctgac aggagaatat gattctgcat ttgctgcaaa aaaattgcaa
    14941 gatttcttat ggaaagacgg tgaaccattt aattatgaat tggacaatat tcctagaaca
    15001 caggatttgc cgcctttata cgaagaaact agtggatttt atatttataa tcgcaatgtg
    15061 atgaccgagt tgcatagaag aattgggaaa aaaccttgca tagttgaagt aggtgagatt
    15121 gagtcagttg atattgatga agccgaggat tttatgattg ctgatgcgat ttataatcat
    15181 cttattttac aaggtagtta agacaggggg aaggttagga tgaacaatgt acagatttta
    15241 gattgcacac ttagagatgg gggctattgt aacaattggg aatttggatt tgataataca
    15301 aaaaaaatta tttctggact tatagaatca ggaattgaga ttatagaatg tgggtttctc
    15361 acaaataaaa cagtatacaa taaaaatgtt acaaaatata cgaccttaga agaattagca
    15421 cagataattc ctgaaaaaag agaaggaaaa ttatttgtag caatgatgaa ctatggcgaa
    15481 tataatatag aggatcttcc agattatgat ggttcatcta ttgatggaat taggattgcg
    15541 tttcataaaa aaaattgcaa tgaagcgttg aaattatgtc gtgagataaa gaaaaaaggc
    15601 tacttagttt ttgttcaagc tatggtatct ctcaattata cagatgaaga gtttttagca
    15661 gtaataaaaa atgtgaatca gtttaaacca tacgcttttt atattgttga tagttttgga
    15721 atgatgaaag gaaaagattt aactcgtctt ttttacatga ttgagcataa tctagaaaaa
    15781 gatatacaga taggttttca ctcacataat aacatgcagc ttgcatattc taatgcacaa
    15841 aaacttataa ccgtacaaac aaatcgaagc ctgattattg attcttcggt gtacggaatg
    15901 ggaagaggag caggtaatct taacacagaa ctatttgtag aatatttgaa tgataatgca
    15961 ggaaaaaaat atcaattaaa accattatta tatataattg atgaaatact aaatagtttc
    16021 tatcagaaga gttattgggg atattcatta ccaaactata tttcagcagt tcataatgct
    16081 catcctaatt atgcaagcta tctgtcatat aagaatactt taacaattga agcaatgaat
    16141 gaaatattcg aaatgatgga tgaagaaaaa aaggtatctt atgataaaca atatatagaa
    16201 gatttatact acaaatatat ggaaactggg aaaatacagg aggaatatat ggaagaactg
    16261 agaaaatttc tctcagacaa aaaaattcta ctgattgcac caggaaaaag ttctgtagat
    16321 gaggtagaaa gaataaaaga gtttgttacc aaagaagaag tcgtaataat tagtgtaaat
    16381 tttgaatatt ctcatataga tatagattac gtatttttaa gtaatttaag acgttttcat
    16441 gaattaccga aagaaagcag aactaaatgt attgttacat caaatataca agcatcggat
    16501 gtatatttac ggacgagtta taaaaagttg ttatctgaaa gggaagttgt aagagacaat
    16561 gcaggattaa tggcaattag atttttagcc gatatgaatg tggatgaaat attcttagct
    16621 ggatttgatg gatattcaca taatgaagat gagaattatg gtgagcaatc catggaaatt
    16681 ataacaaaat ttgcattatt agatgcaatg aacgtgcaaa tgagagatga attgtcagaa
    16741 ttagctaaaa aagtaaaggt gacattttta acggatccac gttgggttcg aattgataag
    16801 tagtagtaat aatgttttta aaattttgat aaagagatag tgagagaata aaaatgggta
    16861 aagtcttaaa taaaaaagaa gaaaaatatt taagggccga tggggcgaga tgaaactcta
    16921 attttgaact tttaagaatc atttctatat tgtgtattgt tgcttaccat gctaatttgc
    16981 gtttaaatta taatgcagac ggaattatag tacatggttc attaactgct aaccagattt
    17041 tcttgaatta tattggtatt tggggtcagc taggagttaa ttgttttata tttatttcgg
    17101 catatttttt aagtgagaaa agaaacattc actttgaaaa tgttttggga attgtattag
    17161 aatgtatttt ttatagtgta ttagtttatg gaattctttg tgcatgtgga atagaaacat
    17221 tttcattgaa cagctttgta aaagaaatat tatcatttgc tctaggtaga tattggttta
    17281 ttacatccta tttagcattg tatattctaa gtccgtattt aaatatcatg cttaacaatc
    17341 tggaaaaact tcagttgcaa aaaataagta tgttattgtt tataggtgta tgctgttatc
    17401 gtatgttatt taaaacatct ttgtttgggg attgtgctgt tttaattgca ctatatgtca
    17461 tatgttatac actgaaaaaa acgataataa tatttttgag agacacagaa tcataggaac
    17521 aattgtatgt ataacagtta taccacttct catatccata ggaatacttt tcaaatttat
    17581 accaaatgca atggttaacc agatggttca tattggttct ccattgatgg ttatagcaag
    17641 tatattttta ttttatgtat ttaaaaattt ttcattttac agtaagtata taaataaact
    17701 tgcgagaacc agtttagggg tatatatttt acatgaaaat cattatttca ttgatggtat
    17761 gaagtctaga ggcttaatat gggatgggat atttggatta ggcaaaaggt ttttagatag
    17821 ggatttttgg atttggttta taattgcagt tgtagttgtg tgcataattt gcatggctat
    17881 cgatgttgta agagaaaaat ttgaaagaca gatttttaaa attaagagtg taaagaacct
    17941 gtgtaaactt atgaatcatg ttataaatat atacccataa ttaataaatg aggagaaaag
    18001 aagtgaaagt tatatcattc ggagaattat tattgagact tgcagcaccc ggttattcaa
    18061 agttatttca aagagatagt ttggagacta cattctgcgg aggggaagct aatgtagcgg
    18121 tatccttagc aatatttgga tatcaatcag aatttataac aaagcttccg gattcagatg
    18181 ttggatatgc ggcagctaat gctttgcgtt attttggagt aaatgttgat aatattatat
    18241 ttggagaggg acgcatggga ctgtattatt tagaaaaagg agcagctcag agaccatcta
    18301 aagtaattta tgacagaaaa ttttctgcaa tttcgttagc tgatagtgat gaatttaagt
    18361 ggaaggagat cctaaacgat gcaaattggt ttcattggac agggataaat ccagcattgt
    18421 ctgagaatct ctttgagacg tccttagatg catgtcaggc tgccaaaaaa agaggtataa
    18481 caatttcttg tgatttaaat tatcgatcca agctttggac atcagaggaa gcaaaagaaa
    18541 agatggaaca gatactacca ttggttgata tttgtatttg taatgaagaa gatgcagaaa
    18601 aagtgctagg aattaaagct gagcatacaa atattaatag tggagaatta aataaagatg
    18661 gctatgtgca ggtggctagg gagattcaca agaagtttgg atgtaggtat gtagctacaa
    18721 ctctgcgtag aagttattcg gcaagtagaa atggttggag tgcaatgtta tacgaagcag
    18781 aaaaagataa agcgtatttt tccacagaat acaacataca aattgttgat cgcgttggtg
    18841 gtggagacag cttttctgct ggtataatat atgcgttatg tgaaaatatg tccggacaaa
    18901 aggctattga atttgcgact gcatctagtt gtttaaaaca tactatagaa ggtgatttta
    18961 atcgaactac aattgaggat gtatataatt taatgaatag cggtggaaat ggtcgcgtgt
    19021 tgcgttagtc aacatataaa gataaatcac agtaattttt atggtgatta tctgtcttag
    19081 tagtgattag aataacgtga aaataaattg ttgagcgttt atactgtttt gaaaaataaa
    19141 tttaacatat tgaaaaatac aaatatatac gtatattata aagcgccgat tgtaaaatta
    19201 agctagacaa ctaaaacagt catttgtgct acactcaaaa tagttccgac caaagaatta
    19261 taaggagtga gtgcaaataa cctataccta tcttacaacg gatgaacttg taataataga
    19321 gtcttatttg aaaattaacc aatctgttgc taaaactgcg cattgcttga atcgttcaag
    19381 tcaaacgatc cataaagtat atctgttctt caagcaaaga aaatcagcct tagaatacta
    19441 tcaacagtat aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt tacc
//
LOCUS       DACEOM010000058        19305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEOM010000058 DACEOM010000000
VERSION     DACEOM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.059"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            238..1629
                     /locus_tag="IW781_002040"
     CDS             238..1629
                     /locus_tag="IW781_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1183034.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1688..2491
                     /locus_tag="IW781_002041"
     CDS             1688..2491
                     /locus_tag="IW781_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1183035.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2524..3378
                     /locus_tag="IW781_002042"
     CDS             2524..3378
                     /locus_tag="IW781_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAQ1183036.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3375..4076
                     /locus_tag="IW781_002043"
     CDS             3375..4076
                     /locus_tag="IW781_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAQ1183037.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4084..5151
                     /locus_tag="IW781_002044"
     CDS             4084..5151
                     /locus_tag="IW781_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1183038.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5144..6109
                     /locus_tag="IW781_002045"
     CDS             5144..6109
                     /locus_tag="IW781_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1183039.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6154..7128
                     /locus_tag="IW781_002046"
     CDS             6154..7128
                     /locus_tag="IW781_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1183040.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            7199..8686
                     /locus_tag="IW781_002047"
     CDS             7199..8686
                     /locus_tag="IW781_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAQ1183041.1"
                     /translation="MFIYILRIVIQFVGRSFFIYFLGAKYLGLNGLFTNILSFLSLAE
                     LGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKN
                     STDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIA
                     LYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIG
                     NMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGN
                     FAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILI
                     VLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIG
                     TIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGG
                     STFLQFITKTLITIVTTSILYLLLYFRREEFTFIKKILFRYILKK"
     gene            8743..9858
                     /locus_tag="IW781_002048"
     CDS             8743..9858
                     /locus_tag="IW781_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1183042.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            9855..11270
                     /locus_tag="IW781_002049"
     CDS             9855..11270
                     /locus_tag="IW781_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ1183043.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            11303..12250
                     /locus_tag="IW781_002050"
     CDS             11303..12250
                     /locus_tag="IW781_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1183044.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(12290..13450)
                     /locus_tag="IW781_002051"
     CDS             complement(12290..13450)
                     /locus_tag="IW781_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1183045.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            13615..14790
                     /gene="mltG"
                     /locus_tag="IW781_002052"
     CDS             13615..14790
                     /gene="mltG"
                     /locus_tag="IW781_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1183046.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            14926..15405
                     /gene="greA"
                     /locus_tag="IW781_002053"
     CDS             14926..15405
                     /gene="greA"
                     /locus_tag="IW781_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1183047.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            15573..16304
                     /locus_tag="IW781_002054"
     CDS             15573..16304
                     /locus_tag="IW781_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1183048.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            16301..17368
                     /locus_tag="IW781_002055"
     CDS             16301..17368
                     /locus_tag="IW781_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1183049.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEESETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            17375..18007
                     /locus_tag="IW781_002056"
     CDS             17375..18007
                     /locus_tag="IW781_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1183050.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            18147..18812
                     /locus_tag="IW781_002057"
     CDS             18147..18812
                     /locus_tag="IW781_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1183051.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            18840..19133
                     /locus_tag="IW781_002058"
     CDS             18840..19133
                     /locus_tag="IW781_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1183052.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(19201..>19305)
                     /locus_tag="IW781_002059"
     CDS             complement(19201..>19305)
                     /locus_tag="IW781_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1183053.1"
                     /translation="DFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         6739 a         2703 c         3715 g         6148 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga
     1561 ctagatgaag atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt
     1621 tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa
     1681 tgtaagtatg ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt
     1741 aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc
     1801 tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga
     1921 acatacgaaa aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg
     1981 attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa
     2041 tattgaggaa tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata
     2101 taacaatgca attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat
     2161 gtttgataaa aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga
     2221 ttattatctt tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac
     2281 attagtctat gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct
     2341 tggttctggt cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga
     2401 ttttataatt gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg
     2461 aaaattttat caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca
     2521 aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt
     2581 ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa
     2641 caagggttta ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa
     2701 aaattttatc aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct
     2761 tctaaaaatt ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt
     2821 attaaaactt atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg
     2881 ttagatgttg cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta
     2941 tatgctttat tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt
     3001 agtaagatac tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt
     3061 gctgaaaaac aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt
     3121 gtcggaccta aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg
     3181 gtgccattcg aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa
     3241 gtttttggtg actatatgca gcttccgcct gaaaaagatc aggtttctca tcatgaagca
     3301 gtttatattg atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa
     3361 ggagcagaaa aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg
     3421 gacaagatat cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt
     3481 tagaatcatt tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt
     3541 gggaagaaac gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca
     3601 ttacaggtgg atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact
     3661 attctgaaga tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac
     3721 tggtattgtc agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac
     3781 cttacaacga acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc
     3841 gtaacacatt gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag
     3901 catatgaccg ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca
     3961 tgatggtaga tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat
     4021 tgacaacaac agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga
     4081 aagatgatga cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat
     4141 catacaccaa aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga
     4201 atgatcggct cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa
     4261 atcaaagttt atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat
     4321 cttgaattag atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa
     4381 attcaagctg actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact
     4441 gatccaatcg gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca
     4501 gtagcaaccc aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat
     4561 cgaggcgata tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg
     4621 agagcaggct accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca
     4681 aaacacgacc tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt
     4741 ttatctgata cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt
     4801 gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct
     4861 gctattcttc atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa
     4921 aactttgatt tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa
     4981 gtaatttttg aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca
     5041 gtacttgaaa ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa
     5101 tcattggttc atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat
     5161 tattgttcct gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc
     5221 ccaaacattt actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg
     5281 gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc ataaagaaaa
     5341 cggcggatta agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg
     5401 ctttgttgac agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt
     5461 gaaaattcat gatgccgaca ttagctctgt tgaattgatt cctttttatg gtgatagata
     5521 taagaaagct aataaagaaa agaaagtaat tatattaaat aaaaaggaag caataaaatc
     5581 tgttttagaa ggaacgcaat tttatgcata cgcatggaat aaactttatc gtaaggaact
     5641 ttttaaagat aatcgttact tggatggaaa aacatttgag gatgcatata taataataga
     5701 tctattattt caaacagaaa aaattgttgt ttccaatgaa gagaaatatt tctatcttca
     5761 aagaaatgat agtattatgg gaaaaagttt ttctatgaat aatttcgatg ttatagaagc
     5821 atggcaatat aacaaagaga agattttaga tgcttttcca gatttgcatg attcctatta
     5881 tcgccgacta tgttgggcat atttttacgt gttggataaa atggttttat caagtagtta
     5941 tcaacaaatt ccacgtaaaa aaacagtaat tgaattttta aagaagaaca gaaattttat
     6001 attaacttat aagggattca ctaaatcgcg caaaatagct attattgctc tgagctttag
     6061 agtagattgg tataggaaat tggttgaaca aaaaaataaa aaaaattaga atgtttaaat
     6121 tagtaaataa aagaaaagga attgaaaact aaaatgtgca gaataagtat aatagttcca
     6181 gtttataatg ttgaaaaata tttaaaaaag tgtgtcgatt caattctagg ccaaacattt
     6241 accgattttg aattattatt aattgatgat ggttctacag ataattcagg aagtatatgc
     6301 gatgaacttg cgaaaacaga caaccgaata aaagtgatac ataaggaaaa tgggggtcta
     6361 agtgatgcca gaaatattgg tattgaggta gcaaagggag attttattgg gtttatagat
     6421 agtgatgatt acattgatga agatatgtat gcatttttat ataacaatat attgaaatat
     6481 gatgcagagt tatcaatgtg tggtatttat gacgtccata agaataaaga aattaaaaag
     6541 ttaactcctt tttctcaatt ggttactaaa tctgaagcaa ttgaattagt attagatggt
     6601 aagctagttg ttgctaatgc agtaagcaag ctatataaaa aagaattatt tgagaatgtg
     6661 agatatccaa aaggaaaaat tgctgaagac gcagcagtaa tactgaaaat tatcaatcag
     6721 tgtaataaaa tccatgttga tacttctcaa aaatattatt attatcatcg tggagatagt
     6781 attactagta aaaaatttac aaaaaaagat tttgatataa ttgatgtatg gaatgagaat
     6841 gaaaaatgga ttattaaaaa ttatcctcaa atgtacgata aagtacatac tagagtttgt
     6901 tgggcttact ttgttgtgtt agataaatta gttttaactg aagaaaaagg actgtatcaa
     6961 gaaaaaaaac aagtaagaga gtatttaatt gataatttct tttttattat aaaaaataaa
     7021 cgattaactt ttcaaagaaa aatatctatg ttattgctta cttttggaat tgctgcgtat
     7081 aaaataccgg ttaaatatca gtataagaaa gtaaaggctg ttaattaatg ctttagttag
     7141 gatgaattag atgaaaaaaa gcaggttaga gtattcttta ataaattctt ctatatcaat
     7201 gtttatatat attttaagga tagtaataca atttgtaggt agaagttttt ttatttattt
     7261 cttaggagcg aaatatttag gattaaatgg tttatttaca aatatactta gtttcttgtc
     7321 gttggctgaa ctaggcatag gctcttctat tatttattcc ctatacaaac cattagccca
     7381 aaaggatgaa agtaaagtaa aggctttaat gaatttgtat aagaagacat atgaatttat
     7441 tggtattttt atcggcatag taggagtatt gattattccg ctgttacctc tatttattaa
     7501 aaatagcaca gacataccac atatgtatcg atattatgta ttatttttat taaattcagt
     7561 tcttagttat tttttcacgt ataaaagatc acttgcaata gcagatcaaa aaaattatct
     7621 ggttgcgatt aatgattttg cctttttatt ctttatgaac attgtacaag taattgcttt
     7681 gtacttttat tctaatttta ctctatttct aatagtgcag attttgttta ctctattttc
     7741 taatataaat atttccagaa cagttgacaa gagatattca tatttaaaag agaaagatgt
     7801 agtttctcta gataagcaaa caaaagatga agtaaagaaa aatgtcatag gtaatatgtc
     7861 ctcaaaaata ggcggggtta ttgtaatggg gacagataat atattaattt ctacatttgt
     7921 taatttaaca gctgtaggaa tttattctaa ctattcattg attgtcacaa gtgtgcaaaa
     7981 tttatgtaag caaattacta attctatcac ggcaagtgta ggaaattttg ctgtatcaaa
     8041 taaaaaaaag gattcatttg agctatttaa aaaacatttt tttatcaacc actcactaat
     8101 ctttttttct tctatattgt tattgacttt aatgaatccc ttcattagct tgtgggtagg
     8161 agccaaatac atgctaccta tgtcaacgac tattttaatt gttttaaatt atagtgtaca
     8221 ggtttataga aatacatcct tcgtttttat agagtcattt ggtttatatt ggtctcaacg
     8281 gaaaaagcct ataattgaag caggtgtaaa cttgttatta tctttgcttt tgttaatagt
     8341 ttttgatatg ggaatcaatg gagtacttat agggactatc ggatcgtctc taggatttgt
     8401 gatttggtat gaagcattta tcatatataa aacggtattt gagaaacgtt ttattcattt
     8461 ctttttaact ttctggcgaa gttttataga attaataatt tcggcaatag tcgtttttct
     8521 attaaatgaa tatttagcaa atggaggcag tacgttcctt caatttataa ctaaaacctt
     8581 aataactata gtaacaacat cgatattata tttgttatta tattttagaa gagaagaatt
     8641 tacatttatc aaaaaaattc tttttaggta tattttaaaa aaatagcttt ctataaagga
     8701 gtgttttatt tgaaaatagc tatactatct ccagctttct caatgggagg agctagtatc
     8761 gtggcctcaa aaattgcaaa aggattaata gatagtggcc atgaagtttt ttttgtttct
     8821 tacttagaaa gtgaaaaacc acaagtagaa tctaagtatt atgatttata cgaaaaaaaa
     8881 atttttttat ttaaaaattt ttctcgattt cataagatat tagagtatag attgtttgga
     8941 ggctttgttc ctagtagata tataaaaaaa gaaaaagaac aactaaaaaa agttctaaag
     9001 aaagaaaaac ctgatgtcat catattcaat acttttatac ctagtgtact attctccgaa
     9061 ttcctaaagt atgaatttcc aacagtgaaa ttaattactt ggatgcactc tgatcctgat
     9121 tacagcttaa atcatattgc taaattttat aaaaaagaat atagaaaaag ttttgaacgc
     9181 gtggattcca ttgtttgtct ttcgcaagat acaaaagaaa aactttcatt atacaataac
     9241 catattaaag ttatttataa cccattgaca ttaagcggct ctagtttaat aagtaacctt
     9301 gataaaaaaa ttatttcatt tactgccaga tacgatataa atattaaagg gtttgattat
     9361 ttatgtgaag tagctaaaaa cttacctgtt ggatgggcta taaggattgc tggagatggt
     9421 acagaagaag aacgtaaaga gctgaaaaga ttaattgaga aaaatggtgt gttagataaa
     9481 attatttgcg caggtgcatt atttggagat gaattaatca atcattatat agaaagttct
     9541 attttcatat ctacttctag aagtgaggga ctaccattag tgttgataga agcaatgact
     9601 tatggattac ctgtaataag ttttgatcat ggtggtgcta aggaaatatt acaagatgga
     9661 aattatggaa ttttaattaa gaattttgac gtacaagata tgaataatga gatagaaaaa
     9721 ttgtctaata atcgtttact tatggaaaaa tatcaaaaat tctctttaga aagagctaag
     9781 gattttaatg aaaaaaaaat aattggtgaa tggcaaacat tgttaaatga attaggaaat
     9841 gaggatgtta gaaattgagg acttcgatta aaaattttaa tttatggaaa gaatggtatc
     9901 tatatatatt ttttataggg atgattattc gtgcaataaa tttttatagt ttattaccgt
     9961 ccacttttga tagtattttt tttaaactta taggaatttc aggagttttg ctattaggga
    10021 tagatttcat aattaaattt aataaaagaa aagtaactta taacatttta ttgatagcat
    10081 atatggtgat tttagtaata acgagttttg ttcataaaga atatggtcta ggagaaaact
    10141 taaagactat aatgtgggca atcttacagt attttttgat ttatcagttt gcttttgaga
    10201 atagcgataa taaacgattt tttaaaagga taagttgggg atttatttat agttggtttg
    10261 ttttagtatc aatgtcaatg gttcttttct ttggtaaatt cggctatgag aaatattata
    10321 atgctagaca taggatcaga attggttttt tagaatcaag actgtttggg acgttttctg
    10381 atattaacaa tgctgctata gcatctttag ttgttattgt cctaatctta ttttatcttt
    10441 ataataaaaa agtgacagga ttaaataaaa tcattttttt aattaatctt ccattacaat
    10501 ttatttatat agtattaagt gggtcacgat ctacctatgt ggtaagtata ataatagttg
    10561 ccatatcatc ttttattgtt gtgttaaaac gaaatggaac aaaaaaagat ttaaaaaact
    10621 atatccttgc tatagtatct tctattggtt ctgttctgat tttattcgct ttgtttaatg
    10681 caactaagat actttttcaa gaattattac ctcatttaaa agaaataaat ttttttaatt
    10741 ttacagagcg tcataaagaa ttaatgaata atagtcaaca tattaataat actcaaacac
    10801 ctgataatac tcttgttagg aaagacgtag ctgaaaatac agatatttct aatgctagaa
    10861 ttactatatg gaaaagtgca tgggaaatat ttaaaacaag ttggcttatc ggagtctctc
    10921 cgaaaaatgt agtatcttat gcacagtcaa ttattccaga tggatatatt gcgaaagtag
    10981 gaattgccat tcataatgca tacttgaatg tattaacatc gacaggtatt ctaggaataa
    11041 ttcctttcat ggctttttta ataaagagtt ttgtgctgaa tgtttatcga atcatctata
    11101 ataaatgcat tggtaaagat tatcattttg tttactgttt agttatttta tcttatgcac
    11161 tatacgcagc tcttaacaat gaattagtct atgaaaacac tgtaggaacg tttgtttttt
    11221 ggctattttt aggaagagtt aatagcaaaa ataattctag ctatttttag taataaattt
    11281 tatcttgaaa agaggagaaa aaatgttgaa ttacttgatt accggtggcg ccggcttcat
    11341 tggctcgacg ctcgcgaatt atctaggaaa agaaaacaat gtagttgtga tcgatgattt
    11401 atcgatgggg aaaaaagaga atctggatga atcgaagcac atcacgttta ttgaagggga
    11461 tgtcgctgac aaaaatttga tggaaaaggt catgaaagaa taccatttcg attatatttt
    11521 ccatctagct gctgtagcta gtgtggcaga ttctgtagaa cgtcctctag aaactcatcg
    11581 tgtaaatttt gacagtgcgc ttttattgct tgaattggta cgaaaatatc aatctggttt
    11641 aaaacgattg gtatttagtt catcagctgc agtttatggc gatgaaccaa cgttgccaaa
    11701 aaaagaagaa tcggtcatcc gtcctttaac accatatgcc atcgataagt ttgcagcaga
    11761 acaatatgtt ttggattact gtcatttata tgatgttccg actagtgctg tccgattttt
    11821 taacgtttat ggtccaaatc aaaatcctaa ctctccatat agcggagtta tttcgattct
    11881 agtggaccga tacaaaaaac aattggcagg agaaaaaaca gaatttactt tatttggtga
    11941 tggtagccaa tctagagact tcgtttatat agaagatgtt atacaagcac ttttacttgt
    12001 tgctaaggaa gaaaaagctt taggacagca attcaatgtc ggtacaggaa aaagcactac
    12061 gctacttgaa ttgattcatt caattgatca aatcttagga acagagctag cgcttaaata
    12121 tgaagcggag cgttcagggg atattcgtga ttctttagcg gatatttcaa agatccgttc
    12181 tttaggttat caacccaaat ttgatatatt gaacggaatg gaacgttatt taaagacaga
    12241 aatcgattga aacaaaaaac cagcatcctg ctggtttttt tgtctcgact tagcttttta
    12301 aattaacacc ttccatcagt tcgtaaatct gatttcttaa atcagtggcc ggcaatttga
    12361 tgtttttatc agattggata ttactttcca tgacgattgc atcttttgtg tcttttttca
    12421 tcgccgctac tgcatggaat cgattgatat ttccctgagc aaggatataa tcatttccac
    12481 tgtaaacacc gccgctgtaa ggatgtttat caccattatt ttgccacaat gagaaaagta
    12541 gttcttttgg tataatatcg ccagacagaa cactggtaaa gaatttgttc aagtcataaa
    12601 cagacatact tatatttcct gtgcctaatt catttcggat gtctgtttca ggctcgacca
    12661 atgcttttga ataatcgtca gttgcagaca tttggtaaga tacagcatgc tgaggggaat
    12721 tttttacatt ttcataaaaa tctgtatgtt ctagatggag tggccgaatg atttcttttt
    12781 ttaaaagagt ttcataaggc tgattcatta gtttgatcaa tacccctgta agcaatgtat
    12841 aatttaatgg ttcataatga tacgtatcat aaccagtaaa tttcaaatga tgaagggcaa
    12901 actgaatgat ctcactatct gacataggtt tttttggaga agcggttcga tgaagaccag
    12961 agcgcatgta aagcatattt ttgattgaga tttgctgact tccaggaatc tgaggataaa
    13021 attcagatag ctttgtgtca aaagttatct tgccagattg aatttgtttt aggataagta
    13081 aagcagtcat cgctttttgg atcgaaccga tttgatagta aagagaagga gaattcgatt
    13141 cttttatgcg tagatttgcg tacccatatc ctttttctaa tatcactcga ttatttttgc
    13201 gtacataaat cgaaccgata taatctgatt tttgcagtaa ttgatcaatt ttgctggcaa
    13261 gcatctgctc attagatgtt aaattggaag caagttgtgc gggttccgtt ttataaattt
    13321 gtggttgagg ttgttttgtt aattgccatc ctgtaatgat gcctgttgaa aagaagctga
    13381 cagccagcaa caaaactgcg agaaaagaaa gagagatctg ctgttttttt tgatgttttc
    13441 cttttgccat attaaatcct tcctatccat ttttaactat ttttcattgt aatcctcaaa
    13501 aagagcattt gccacttaca attttgacat aatatttggt aaaataattc acggcagtag
    13561 aaatttcatg tataataaac agagttacga agatgatagt aaggaggagt tgttttgtca
    13621 ggaaacaaac aacacccaag caaagacaaa aaagaagcca gtaaggcagc tacagcagat
    13681 ataatcgaca agcggagaaa tctgcgtaaa aaagaagata agatcgtgag gaagatcatt
    13741 ttagtgatcg ctcttacatt attgattatt ggcggatttc tcgggtttac cgtatatcgt
    13801 tatgtagata gcgggttgaa gcctttagat aaatcggacg accaattagt ccaagtggaa
    13861 atcccaagcg gctcttctaa taagcagatt ggcgaaattt tggagaaaga caatattatt
    13921 aaaagtggga ttgttttcaa ttattataca aaatttaaaa acttaactgg ctttcaagct
    13981 ggttattatc agttagcgcc taatatgact cttgatgaga ttggcaagca gttgcaagaa
    14041 ggcggcacat ctgagccaac aaaagttgca gatgggaaaa ttgccattcc agaaggctac
    14101 gacatcgatc agattgctga gcgggtagcg aaggtaactg gtaaagacaa gaaagaattt
    14161 ttagatttag taaatgatga gactttcttt aacgagttgt atcaaaaatt cccagaattg
    14221 ttggaaagtg cctctaaagc tgagaacaca aaataccgtt tggaaggcta cttattccca
    14281 gctacttatg attactacac gagtacttcg cttaaggatc ttgtgattga gatggtgaac
    14341 aaaacaaata cggtcatgca aaactattat tcagctatca aacagaaaaa tttgactgta
    14401 caagaagttt taactttggc ttctctagtt gaaaaagaag gcgtaaaaga aaatgatcgt
    14461 aaaaatattg cacaagtctt ctttaaccga atcaaggcaa atatgccact tcagtcagat
    14521 atctctgttt tatatgcatt aggagaacat aaagaattgg tgacgtatga agatacagct
    14581 gtagattcac catacaattt gtataccaat accggctatg ggcctggtcc ttttgacaat
    14641 ccaagcgaag aggcaatcaa ggcagtatta gaaccagcag aaaatgacta ttattatttc
    14701 gtagcagata cgagcactgg aaatgtttat ttcgctaaaa cgtatgaaga acatatggaa
    14761 ctagtgcaaa aatatgtaaa taattcttag aaaaacaggg gctagtcatt gatcattttt
    14821 agaaaaacat ttacatttta agaaaagcgt ggtaatcttt tttggactat attcctcaaa
    14881 agattacctt ttgcggtatt ttatcgaaaa aaggagacgt tacacatggt agagaaagtt
    14941 tatccaatga ctctagaagg taaagaaaaa ttagaaaaag aattagaaga actaaaaaca
    15001 gttaaacgcg gagaaatcat tgaacgaatc aaaatcgctc gcggtttcgg agatctttcc
    15061 gaaaactccg aatacgaatc agctaaagat gaacaagcat ttgttgaagg aagaatcaca
    15121 acaattgaaa atatgatccg ttttgcacaa atcatcgata atgacggcgt agatgcagat
    15181 gaggtatcga tcggtaaaac agttacgttc atcgagttgc ctgatggaga tgaagaagag
    15241 tacacgattg tcggaagtgc tgaagcagac ccgttttctg gaaagatttc caatgattct
    15301 cctatcgcgc aagcattgat tggcaaacgt aaaaatgatg aagtaaccat tgctacacca
    15361 ggtggcgata tgttagttaa aatcgtcaaa gtagaacaag cgtagtctga ctgaaagatc
    15421 tagagagttc atcttactga tgacgcactc tagatctttt ctctttgagt attgcaggtt
    15481 tcactgaacg atcttacatc taaagtccta tagccaacag tcaaaaaaat aggtatgatg
    15541 aaatgagaat gtgtcaggag ggagaaaaag cgatgaatag ttcttggcgt ttttttgtgg
    15601 tagtcgaagc actgctgctg ttatttgctg tttggcaaat cgtaaacaat actggacttt
    15661 tgcttttagt aatattcggt attttcaata tatatctagc tatgcgcaaa tatcctcgta
    15721 ctaaatttca aaattttcaa ttgatcttag ggagcttggt catttttttt agcttagtca
    15781 acagtcctgc tttatggatg atggttgtat tggccgtttt atttatcggg ctgaaaggtg
    15841 tagagatttc aggaatagat ttgacaaaaa atgctttttg gcgaaaaaaa cagatcatga
    15901 tggtccaaac agagcaatta aaaacacata ataatgagcg taaaagacag caattatttg
    15961 gtaaccaacg tattggaaac gacgtctatg aatgggatga tatcaacatt gccatcattt
    16021 ctggtgatac tatcatcgat ttgggcaata cacttttgcc aaaagatgat aatattgtga
    16081 ttgtaagaaa agggataggc agaacacgga tcttagtacc cctgggagtg gctattcgtt
    16141 tagagcatgc gacattagta ggaaatgtct tgtttgaaga agagcagttt tccttaaaaa
    16201 acgaacagat caaaatctgc agcagtgatt atgatgaaaa tcctcgcaga ttgaagatca
    16261 ttaccaatac tttggttgga gatgtcgagg tgatacgaat atgatgggaa gaatatccaa
    16321 agcaatgcta gctgtttatt cggggattgc ggctttcctt attattctat tttcactttt
    16381 cacttatttt tatgccagta accaaagtca ttggtgggga gaattgctgc gtgcgcgttt
    16441 attatacgtt ccgcttatct tccatttgat ggcaatttca ttaggtgtag gattgatcgt
    16501 atttctattg ttatcactta ttcaaaaggc aaaatacggg aaaatcgaag aaaagcttcg
    16561 tgcactttct tctggcaatt atgaatccaa acttttgcat cagccgatcc caagcgcgtc
    16621 ggatgatctg tacatcaaag atattgatat ggaaattact aaaataaaag aaaaaatgat
    16681 cgaagtatcc agtgaattac agattatgac tagccgtcct caatatgtag atggacaaac
    16741 caaagaagag attttaaaat tagaaaggca tcgattagct cgtgagcttc atgattcagt
    16801 ttcacagcaa ttatttgcag cgatgatgat gatgtcggca ttgacggagc aagcagaaga
    16861 aagtgagaca ccagaaatgt tccgtaagca gttgaaaatg gtggcagaga tcatcaacgc
    16921 ttcccagtct gagatgcgtg cgctgcttct tcatctgcgt ccagtcaatt tagaagaaaa
    16981 aagtttaaaa caaggcattg agcagttatt gaaggaattg cagaataaaa tccagatttc
    17041 gctaaaatgg gaggtagaag atgtaaaatt aactagttcc attgaggatc acttgttccg
    17101 aatcgttcaa gaattgttat caaacacatt aagacatgct aaggctaatg aattagaagt
    17161 ctatttgcat aaaatagata acaatctttt attacgtatc attgacgacg ggacaggatt
    17221 taatatgaat gaaaccaaaa cgggaagtta tggattgaac aatatcaaag aacgagtagc
    17281 tggtattggt ggaacagtaa aaatcattag ttttaaaggg caaggtacga gcgtggagac
    17341 caaggtccct ttgatgaagg aggcatagat aaagatgata aaagttttat tagtagatga
    17401 ccatgaaatg gtacgcttag gcgtctcttc ttatttatct atccaagaag acattgaagt
    17461 gattggagaa gctgaaaatg gacgtcaagg ctatgaaaag gcgatggcac ttcgaccgga
    17521 tgtcatttta atggatctag tcatggaaga aatggatggc atcgaatcaa caaaagcaat
    17581 cttaaaagat tggccaaaag ccaaaatcat tatcgtcaca agttttattg atgatgaaaa
    17641 agtttatcct gcaattgaag caggagcagc aggatatttg ttgaagacgt ctacagcaca
    17701 tgaaatagct gatgctataa gggctacgca acgcggcgag cgagtactag aacctgaagt
    17761 gacaacaaaa atgatggaaa aaatgagtcg gcgaaatgat ccagtattgc atgaagaatt
    17821 aacgaatcga gaaaatgaaa ttttgatgct gatttctgaa gggaaaagta atcaggaaat
    17881 cgcggatgaa ttgtttatca cactgaaaac agtcaaaacg catgtgtcga atatattagc
    17941 taagttagag gtagaagacc gaactcaagc agctatctat gcgtttaaac atggtttggt
    18001 gaaataaaaa cgatgataaa gaggataatc aaaagctgag aaaataacta ttatttttct
    18061 tggctttttt acaataatga tgcttgcttt tgtttttgga aaggctttgt tatactgaga
    18121 aaagaagata ctaaaggagg aaaatggtga aacaaaattt tgcaattgta ggccttggac
    18181 gatttggcgg aagcatctgc cgtacactga tcgaagctgg acaagaagta ctggctattg
    18241 acagtaatga agatcgagtc aatgaatata tgaatatagc cacccatgct gttgtggcaa
    18301 atgcacagga tgagatgaca cttcgttcat taggtataag aaatttcgat catgtaattg
    18361 tcgctattgg agaagatatc caagccagca tcttagttac tctgatggta aaggaaatgg
    18421 gcgtaccgaa tattttagca aaagcccaaa atgagtacca tgcccgtgta ctagagaaga
    18481 tcggagcaga tcgtgtcgta caccctgagc gagacatggg gatacggatt gcacataact
    18541 tagtatcaaa gaatattcta gactacttag agctgtcaga taaatattct ttagcagaaa
    18601 tccgagtctc caatcctaaa tttttcaaca aaacattagc tgaattaaat ttcaggcaac
    18661 gttttgactt aacagtagtc gccatacgaa gagtagatca aacggttatc gcttctcctg
    18721 cagcagatga atatgttcgt gaaaatgata atctactggt tattggagag acaggcgatg
    18781 tggatattct agacgataag atgaatcaat agaaagaaaa gagaaagtag gttgttggga
    18841 tgaaattaac ggtaacgcca aaagcacaaa agtggtttga ctcagaaatt gatctgccag
    18901 aaacttacgg aatacgtttt tttggaaaag tctacggcaa aacagaggtt catgatggtt
    18961 tttcgattgg aatgtcagtt gaacagccag aacatcctgt aaagaaagtg gaaatggatg
    19021 ggacactttt ttttatcgag gaagcagatg aatggttctt taaaggatat gatttacttg
    19081 ttgattatga tcccaacctg gaggagccaa cctatcactt taaaaaacaa taaaaaaaga
    19141 gagggtgaca caatttttgt caccttctct tttttctttt gaaaatattg ttttcagcta
    19201 ttaatatcct gcagacaatt ttacttgatt ggtttgaagc tcatgggtgg caaagtagct
    19261 tttcagattg gttaaaaaaa tagccattaa tttcttttga aaatc
//
LOCUS       DACEOM010000059        19270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEOM010000059 DACEOM010000000
VERSION     DACEOM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.060"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..362
                     /locus_tag="IW781_002060"
     CDS             168..362
                     /locus_tag="IW781_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183054.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IW781_002061"
     CDS             631..930
                     /locus_tag="IW781_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183055.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IW781_002062"
     CDS             complement(1027..1461)
                     /locus_tag="IW781_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183056.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IW781_002063"
     CDS             complement(1693..2403)
                     /locus_tag="IW781_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1183057.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IW781_002064"
     CDS             complement(2393..3253)
                     /locus_tag="IW781_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1183058.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IW781_002065"
     CDS             complement(3258..3896)
                     /locus_tag="IW781_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1183059.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IW781_002066"
     CDS             complement(3911..4210)
                     /locus_tag="IW781_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1183060.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IW781_002067"
     CDS             complement(4253..5737)
                     /locus_tag="IW781_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1183061.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IW781_002068"
     CDS             complement(5757..6218)
                     /locus_tag="IW781_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1183062.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW781_002069"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW781_002069"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1183063.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IW781_002070"
     CDS             7521..8291
                     /locus_tag="IW781_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1183064.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IW781_002071"
     CDS             complement(8564..9391)
                     /locus_tag="IW781_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183065.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IW781_002072"
     CDS             9959..11194
                     /locus_tag="IW781_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1183066.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IW781_002073"
     CDS             complement(12193..12453)
                     /locus_tag="IW781_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1183067.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IW781_002074"
     CDS             complement(12480..13781)
                     /locus_tag="IW781_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1183068.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IW781_002075"
     CDS             complement(14073..14531)
                     /locus_tag="IW781_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1183069.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IW781_002076"
     CDS             complement(14544..15869)
                     /locus_tag="IW781_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1183070.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IW781_002077"
     CDS             complement(15886..17298)
                     /locus_tag="IW781_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1183071.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IW781_002078"
     CDS             complement(17315..18079)
                     /locus_tag="IW781_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1183072.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IW781_002079"
     CDS             complement(18183..19058)
                     /locus_tag="IW781_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1183073.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6137 a         3956 c         2954 g         6223 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa cctgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa
//
LOCUS       DACEOM010000060        19188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEOM010000060 DACEOM010000000
VERSION     DACEOM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.061"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            73..501
                     /locus_tag="IW781_002080"
     CDS             73..501
                     /locus_tag="IW781_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1183074.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            488..730
                     /locus_tag="IW781_002081"
     CDS             488..730
                     /locus_tag="IW781_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1183075.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1030..1245)
                     /locus_tag="IW781_002082"
     CDS             complement(1030..1245)
                     /locus_tag="IW781_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183076.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1352..1666
                     /locus_tag="IW781_002083"
     CDS             1352..1666
                     /locus_tag="IW781_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1183077.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1770..2948
                     /locus_tag="IW781_002084"
     CDS             1770..2948
                     /locus_tag="IW781_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1183078.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3351..3644
                     /locus_tag="IW781_002085"
     CDS             3351..3644
                     /locus_tag="IW781_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1183079.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            4487..6814
                     /locus_tag="IW781_002086"
     CDS             4487..6814
                     /locus_tag="IW781_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1183080.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(7006..8526)
                     /locus_tag="IW781_002087"
     CDS             complement(7006..8526)
                     /locus_tag="IW781_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1183081.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8769..8954)
                     /locus_tag="IW781_002088"
     CDS             complement(8769..8954)
                     /locus_tag="IW781_002088"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1183082.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8938..9120)
                     /locus_tag="IW781_002089"
     CDS             complement(8938..9120)
                     /locus_tag="IW781_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183083.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            9225..9554
                     /locus_tag="IW781_002090"
     CDS             9225..9554
                     /locus_tag="IW781_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1183084.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            9594..10598
                     /locus_tag="IW781_002091"
     CDS             9594..10598
                     /locus_tag="IW781_002091"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1183085.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            10595..11041
                     /locus_tag="IW781_002092"
     CDS             10595..11041
                     /locus_tag="IW781_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1183086.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            11191..12006
                     /locus_tag="IW781_002093"
     CDS             11191..12006
                     /locus_tag="IW781_002093"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1183087.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            12212..13384
                     /locus_tag="IW781_002094"
     CDS             12212..13384
                     /locus_tag="IW781_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1183088.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(13546..14040)
                     /locus_tag="IW781_002095"
     CDS             complement(13546..14040)
                     /locus_tag="IW781_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183089.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(14037..14231)
                     /locus_tag="IW781_002096"
     CDS             complement(14037..14231)
                     /locus_tag="IW781_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183090.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            14626..15048
                     /locus_tag="IW781_002097"
     CDS             14626..15048
                     /locus_tag="IW781_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1183091.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(15214..16408)
                     /locus_tag="IW781_002098"
                     /pseudo
     CDS             complement(15214..16408)
                     /locus_tag="IW781_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16632..17009
                     /locus_tag="IW781_002099"
     CDS             16632..17009
                     /locus_tag="IW781_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1183092.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            16997..17335
                     /locus_tag="IW781_002100"
     CDS             16997..17335
                     /locus_tag="IW781_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1183093.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            17469..17912
                     /locus_tag="IW781_002101"
     CDS             17469..17912
                     /locus_tag="IW781_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1183094.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            18174..18989
                     /locus_tag="IW781_002102"
     CDS             18174..18989
                     /locus_tag="IW781_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1183095.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            18999..>19188
                     /locus_tag="IW781_002103"
     CDS             18999..>19188
                     /locus_tag="IW781_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183096.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
BASE COUNT         6411 a         3408 c         3660 g         5709 t
ORIGIN      
        1 ctagattgta gtaataatgg aaagggatag aagtaaaacg agttgaatga attttttgag
       61 ggggagaata ttatgggcga ctcagaggaa acccacattc aacatacgtt tgacagtttt
      121 tgtaagaaag tagtctgcaa tgaggcgtta aatattcaaa agaagtacgc tagatttcgc
      181 caacggcaaa tatcaatgga aatattggaa caaaaagggt taggtacaga gttctattat
      241 ttggaaccaa atttgaatag tagtggtact tttattgtcc ttggtatgaa aatctttatt
      301 tccaatgagg agttggcaaa tgcgattgca gcattaccta gtcaaagaca agaaatcatt
      361 tttatgtcct tcttcatcgg tcttaatgat cgtgaaattg gcgatattat tgggaaaagt
      421 ctcggcagtg tttggtacca acgacaaatg gctttggaag agctaggaaa gtatttgggt
      481 gtcaaatatg aagcaaaata aacgatatat accattcata ccactaacga caatcaaagc
      541 cgcagcttct ggtgattcaa ttgccatgtc catcgttctg tcaaaatatc aaaattatat
      601 ttcacggtta gccatgaaac cagtttatga agaagaaaaa ggaatggtta tgtatgtaga
      661 tgaatatatg cgtagacaac tggaaacaaa attgattgag aagatacttg aatttgatac
      721 aactcgttaa agtatttttt ccctttcctt ttcattctat ggaagttttt tatcgggtca
      781 aaataaagga tttccatagg ctgaaaatag cctagcgctc tttgacaaat gaatagcaaa
      841 tctatcagta catgtagatt ttgattctct agttacgcca tgacaaggtt agcttttaag
      901 cgacaaattt taatatgaga atcgcgacta cgataatgat acttccgtaa ccaaatcggc
      961 ccgccattac tggggggagg tgagattcct atgtgctttg gcaaaacagc tgatagatca
     1021 gaaaaactac tatttccata tttcgatttt ggttggcata tttttcatgc caaattctgt
     1081 gattttaata gcatcggcaa gatttaattt ttcttcagct ctctctccac ttttaagttt
     1141 ttttactgta ctcaagttga ttcctgttcc gaatgctatt gcattagccg tagatttttc
     1201 aagaatttct ttaattattt ttatgtctgc aattccttgt ttcatattaa aaattcctct
     1261 tttctagtcc gttttgatgg agtactggag tgttttttta caattttaag gggtaccgtg
     1321 gtggtcgttt tgattttgag agagaagggt gatggagttg gaagttaaaa caaatgttaa
     1381 ttctctggaa atagagttct attcaatttt acaaaaaaaa ctggaagatt acacggaaaa
     1441 attgcttgca gtcagtcatt tacctctggt attaacaaat actgatttaa agcgactatt
     1501 tcagataagt gatagtacac tgaaccgttt agtaaaactt ggtgagtttc ctccctgttg
     1561 gtacgggatt cgaggacact attttcgtga tgatgtttta gaatggatga gaaagagtga
     1621 ttcgaatgac tatcgagaaa aattgcgact acttcgatct ttataagtta cattttcttt
     1681 ctaagatgcg gtacaatgat gacatagata gaaagatttc caaagggttt ctgcttgttg
     1741 ggttccttca aaataggagg aatcaataaa tggaaaaaac agatcgattc aagaagcaaa
     1801 tcagttctac tggtaggatc acatggtact ttcaagcata tcgaacaaca agacgtggct
     1861 ttaattcaaa acgtgaggcg caactcgctt atctggaaat ggagaaacaa caaaggctta
     1921 agaaaaaaat tgttgcaagc aatgacaagt ttagtgttgt aggtgagcaa tggttccaat
     1981 attaccgttc gttaaatgaa caaaaggaaa taacctatga taagcgagag gaacaattaa
     2041 aaattattgt tcgatggatt ggtgataaaa agttgtctga tttgacacca gagtgccttc
     2101 aagaattgct ttttacctta aaggaatgtg gcgttaatgg aacaaatgtt ggctatgcca
     2161 aaaatagttt acaatcactt attcaagttc taaatatgat ttttaaatat tgtcaaagaa
     2221 agaatttgat aagtgaaaat ccaatgaagt ctgtgagaat gccaaaatat cagttaacgg
     2281 taactgattt aaaagaagcc ataaataata ttgaggacaa gtttcttact gtcgatgaat
     2341 tgagaacttt tttgaattat ggcattgttc atgaggaatt accgatgtct gtacttttcc
     2401 atgttctatt ttacactggc tgccgagtca gcgaggcgct tgctctacag cctggagata
     2461 tagatttcaa aagaaatgaa attctgttct ataagcaaac ggcagttaaa ggtaagagta
     2521 aggattttcg aattgaaact actaagacag ttagttctgc tagacgagtt ctagtaactc
     2581 ctctagttat ggaaaagcta caacagttga ttaaggcttt gggtgagatg aggcaacatt
     2641 acaggtttat tatagaagaa acatatttgt ttgtgtatct tgattctagt aaacgtggtg
     2701 ttccttatcg tcgagagtat gtaaatgatc acgtcaaacg gtgcgtgaat cgctcaggaa
     2761 ttaataagcc atttcatact catttagctc gtcatacaat ggcaagctta gtagcggaac
     2821 actgtagctg ggaagtcttg aaagatcgct taggccatac tgaccaaaca acttcgaaaa
     2881 tttatcgcca cttaacgtca aatgaaaagg taaaacctct gattgctttt agttctttag
     2941 agagttaaaa aaacggtgaa agcactggta ttacaggatt cttgaaaaac gattttttaa
     3001 caaattagtc aatatgtagt cagaggtaat aaaaatcctt ttatatcaat actttaacga
     3061 cttttcacaa gtatcaaaga aatccgtcgt taatcattgt tatcagactg agaaagaacc
     3121 cttggtattc aagggttctt ttttcttagc atgtaatttg aacaccctga tccagttttt
     3181 tctttaatta ttggtagggg agaataatgg attagaataa atctacctat aagtagtagt
     3241 aaaaaaaaga gcactatgat aatatctttc ttaagagtaa ccgctttaat aaaaaatcca
     3301 gaatattcta acataaatgg agtattctta gaatcaaaag gagaaagaag atggtttata
     3361 cttatacgga gcaagaaaag atgaaagttt tagacacatt catgaatgat ggaaaagtaa
     3421 aacaagtacc aagttcaaca aaaaagaaat acatcctatt gaaagaagtc ttgaaacgct
     3481 ttgaccatgg agtgacttat acagaaacag aggttaattc gatactgctg aacgtatttt
     3541 ctagtggcga ttatgtcgaa caaagacgtt atctaatcac atttggcttt ttcaaaagaa
     3601 gttcagacgg tcgggcatat cagatgatgg gaattgaaaa ttagaagttt taatttatac
     3661 tcattgaaaa aagttcaagt atgcgatatt ggttctacac cgcatacttg aacttttttt
     3721 gtcatctgta tctataatac aaagccaaga acgttattca aaatatggat agctatgata
     3781 aaagctaatg agcgattcgc agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg
     3841 tacatgaaaa aatcaattgg atgcgttaca tgaataaaga taattctctt ttatttgtac
     3901 gaataaatac caaaacgtac ctcctaaaat tagattttgg acagatgccc tttaaaaaga
     3961 taacaagatt tcgttcttga aacaatatat atgcttttat tccaatgagg ttgtgaaaac
     4021 agtattttgt cctgagcgtt agaaaattga aaatggggaa tagcaactcg tactttacca
     4081 ttttttaagt gaatacaaga tcagctaatt gaacaccgtt tattcgtgat gagggacaca
     4141 acactgcagt cgacacattt ctcttttatg ttcaaagata acaaatattt aaaacaaaga
     4201 aaaatagtag ataatcccgt cctaactctt ttattcttcc ctcatagctt ccaaatttaa
     4261 gcaatacttc aattaatcaa gttttatctt tttttttcaa taatcgtaaa aaaagagtat
     4321 tttttattta aaaataaaat ctttgtatta aaggacaaga ctaaaataac cttagaataa
     4381 aaagagaaaa aaagccccaa actttctata catcaataaa ataataaaaa ttaaatttag
     4441 aataacgtgt aaaggattat ttgatgattt tttattgaga ggagggatga aaatgattaa
     4501 gaaaactcaa aataaaatcc aacgtatatg tcaaaaacat tttgtttcta gactcaaagc
     4561 tgtatctaaa agaaaatact tacatcccat tatgatttcg atcatcttga tttttacttt
     4621 cttagggagc ttatcaagta tttatgtaaa cgtttataca gaaacgtcaa attttgctaa
     4681 ccaaacgaca gaggagacaa atagtccacc ctattctgaa atacagtcca tagaaagcac
     4741 caatcagtct agcaacaacg agacaacctc aaccacatcc tcgactcaac tgattgatcc
     4801 gacagaaaaa gaaatcacag cagcaaaagc agcaggggag aaagcttatt cagaaacagg
     4861 tagaagccag acatttagta ctgttgcggc cactgcgaat ttcaacggac ataccatacc
     4921 agatggatat acagtgatat atgccaaaga tggagtcatt gttgcggtta acggatcgat
     4981 gagtgcggat aaaactgcgt tacaaggcgt aaccaaagca atcgctttta ttgataaccg
     5041 tccagatatc cataatctgt ttattggctc cacagtaaat ggtactacta tcacattccc
     5101 aaaaagtatc tatatggggg tggctaccgc tggaaatacg atcgattcag ttacacccaa
     5161 tgcggataaa agttattctt ttacaccctc aacatgggga gggcttcctg ccggaggagg
     5221 tatgagtact ttttcaatcg gtatacagtt agcaaatgat attccattca cggggtactc
     5281 gatcaaaaat gggcagatcg ttgatggtaa gggaaatgtt tcaaattttc caacctatca
     5341 agcagcaacg gttcaggtca atgcgaatcg gacaggaact ttttcttata cagtcaatgg
     5401 tggagcagca aaaatcggaa cgtacgggac tacattatct ggaatccatg tgggtgacaa
     5461 agtggttatc actccaacag cagtaacggg ctattctttt gcacaaacaa acggttcttt
     5521 cacagcccta gaaagtaacg tcgatacggt gacctataca ggcaaacaaa caccaatcac
     5581 tattaagtat caagactatt ttggacagat gattgctcct tctaaaataa tgaatcttac
     5641 ttacggaagt gctgcacaag acctgacgac taatacgcct actatcaacg gatatacctt
     5701 tacagctgtt tcagcgatag aaacaaagaa ccaatcagca atgagtgttt tagctagttt
     5761 agatacaaat ggaaatgtaa tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat
     5821 cctatattat aagacaaatg caacagtatc aatcaatgca aatggcagta agtactatga
     5881 tggtctttct gttgtaccga tcgttaaata tgcctttaat gataaaacag aatcaacatc
     5941 ccttttaaac aatgatctca gtattgatca aatcacatgg aatccaactg atttcaaagc
     6001 gatgaatgaa aagggagttg aagtaactgc gcctactgaa attggaacct atacgtcttg
     6061 gcaattgaca aaaagcggac tagctaaact agctgccaga acaaactatt tatttactat
     6121 cgtgcagaca tctgatgtct atacgattaa gcaaatcagc ggtgcggtag agctcagtgg
     6181 cagtaagaca tatgatggaa aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg
     6241 tgtcactagt tcgcttactc cagtacaagt ggcacttagc agtatggatt acacagcaga
     6301 tgcacaatca gcaaaaaata tggttaatgc tggcagctat acaattaaac taacgaatag
     6361 cggaatagac aaagtcaaag cagcaaatcc caagttgtca tttactgata tagccaacac
     6421 atcaggaact tataccatct ataaagctaa cgcagtgatt actgtcgatg acgctgcctt
     6481 taattatgat gcccaaagtc actcgatttc ggttggaaat ctccacgtta ccggtgttgt
     6541 cttaggcgaa tcattggatt ataccctaac agttaacagc cggacagata ttggatcaca
     6601 aatagtaacg attagtttag gcgaaggaag tgtaaacaat aattatacga ttatcgcaac
     6661 agataccgct aagttgacga tcaatccgac tctcttgctt ccttcgacag gtgggatagg
     6721 tctactgccc tttgtctttt taggactgat ctttattttt tctggcttct tttattttat
     6781 ccatcgtaag aaaaaagcag gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat
     6841 atatttgctg ttctcttttt tatcctactg gtctttttta taggttctcc gactgtgtca
     6901 gctagagaat cacctattac atttactgta agcgtgtcat agaatggtac ctaaacacaa
     6961 atagcccgat ttgatacact caaatcaggt tatttgtggc ttatattatt tacagatttc
     7021 ttgtatctgc tgattccatg gcaaataagc gcgtagcgct tcaggtgtca tcgaagacag
     7081 attgggaatc tctgtaaaaa gatgatttaa atatttgcgg atgtttaacc cattcgcaac
     7141 agctgtctgg ataatactca atataattcc atttgatcta gcgcctttta agcttttaga
     7201 aaatagccag ttttttcgac cgatcactaa ctctttgatg gcacgctctg ctcggttatt
     7261 tgaaagttcc aagcgtccat caagcaacac aatctgaagt ccgtctttat gattgactgc
     7321 gtattgaacc gcctttccaa gctgcgattt tggcaaataa gcaaaactct cacaccaatg
     7381 aaaaaattca gtcatcacag gctgcaacgt ttccttcctc gctacaaacc tttcttctgc
     7441 gctaagctta ttaaaggtcc gttcgattcg aaacatcttg tcacagaagt ctaatcctat
     7501 tttggcatga ctttttgggg acggttgttt acccaatgct tcaaaaaact ttctgcgaac
     7561 atgcgcccaa caataaacat tgtttaccgg cagctttcca taggccgcgt aaccatcagt
     7621 atgcaagaaa ccgctaaatg attctaaaaa actttttggc tcatcaacgg aacgcgaatt
     7681 tgcgtgctcg tagagcacaa cttgtttttc ttcgctttta cctgaagcat ataaccagta
     7741 aaaggtcttt tctttatgag tattcaatac ccgatagctt gtctcatccg cgtgtaagac
     7801 ctcctgattg cttaattctt gatgaaggca attgtagatt ggctctaaaa ccatttcact
     7861 taccttgata tgccaattag tgattttttt acgatcaaga gggaaaccaa gtgcatgcca
     7921 ttctttttct tgtcgatatg ccggcacctt tttaacaaat ttttcccaaa tactctgtgt
     7981 cacaattgaa gaggaaccta gactattatt gatcaacggt ttaggaacag gcgctttttt
     8041 gatttttgtt tctcctaatg cttcacattc tctgcaatga taggcatgtt ggtagatgac
     8101 ttgtttttct atatgggcag gaacatacaa tggctcttct ctaagccttg tcgcaccgat
     8161 atcatgtaaa gtatgaccac agtcttcaca caaacaatcg gcacctttta attcataatg
     8221 aataggaact tcaggcagac cttctaatga aagtgctttg agtcccttgc gttttctgcg
     8281 tttaaaagaa gataccgtaa tgggttcctg gctttcttgt ccagtgtgct ctggttcatt
     8341 aaaagttgag tcgtctaata aatcgatttg cccatctacg ggtggtgttt ctttttttcg
     8401 tccaaacaag cgtcgattca tttcagcaat ttgttctcta agaagagtat tttcctgttg
     8461 ctggatttca agcaactgtg ttaattgctt gatttgtaca agaagttcag attccatttt
     8521 tgtcattttt acaccaccat tctagcgatt ttcttacatc tattatacca ttttatgaca
     8581 acgaaaaaca gacaaaatcc ttgatttaac aaggttttcg tctaatatat cgcacctttt
     8641 ttcgtttctt ttagcgtaca tttttcttcg attgaaaggc cagtcagtaa gacttctttt
     8701 ctggaacgcg gccattgaag gtacccactt tcaaatcttt tgtacattaa tacaaatcca
     8761 ttttgatcct agtaaagagc cttgaagcga tctttcttcg taccgcaaaa taagaaaagc
     8821 gcttgatcgt aaacatcaag gtcatattct tctataacga aagctgctag tccatcaatt
     8881 ccttttcgaa gatctgtttt tccacaaacg ataaaaatat gtttcaatgc agttgcatca
     8941 atgagcattg gcattcaact cttttaacaa ggtatacaaa agatatttat ccattccatt
     9001 ataaataaat atttccttat cttccgattt gattctgcaa gctagttttg gcttggtgtc
     9061 aatttgcgat ttttgagccg cacttgaaaa ttgaactggt acaatatctt ttttattcat
     9121 tcgtttttct ccttctatat ttgatagttt aattatagaa gaaagataat cggcgtacga
     9181 gatactatcc tatgacacgc ttaccattta ctttgcataa tcgaatgttc gaaagcgata
     9241 agatgccaag tatcagtcaa aacgacggaa taaatatccc ggataacttt caaggagtaa
     9301 aagggttgaa cggcgttact ttcgctatat atgatgtctc ggatgaattt tacaagctgc
     9361 ggtcagaagg aagtagtgtg gaagacgcac aaagaaaact agcacaaaaa agtgatagtg
     9421 aaaaaatact ggcagaaaat gtgacaaaaa cagtagacga ggaagagggc attgcttctt
     9481 ttagtgcatc ggacaaagat gagcaaggtc gtgacgctgt ttaccgattt acggaaataa
     9541 agacttcaga ttaagtaaaa gaaaaatcag caccatttgt cgttgtgctt cctgtgacag
     9601 attcatctaa tcataaattg acgaccatcc acttgtatcc aaagagcgaa caaaaaatac
     9661 ctgctgcact cacattaaca aaaacagtag agaataagca gacagatttt gcagatgggg
     9721 ataaagtccc ttatctcatc accgtaacaa ttcctgagaa catcaacgag ataggaacct
     9781 atacgatcaa agatacagct gatcctcagt tatggctaga accagaaacg atcgattttt
     9841 tgattggtgg agacaagatt catacatttc acacacaaaa aataaaacat ggttttgtct
     9901 tatcagattc tggaaaagat cttagttcgt ttgctggaaa aaaattaacg atccgttacc
     9961 aaatgacatt gaaagaaaac agtgaaaaaa cgacatttga taacgatgta cgtttgacga
    10021 ctgacaatca aacagtagaa acacatcttg ccatccaaac aggaggcaaa caatttgtca
    10081 aagtggatcg gcaaacgaaa caaaaactag cagatgctca atttctagtc aaaaaaaatg
    10141 accaatactt agcgcaagaa aacgggataa atcattgggt agccaaagat gattcaagag
    10201 caacgatttt tacttccagt aaagatggaa cattttctgt ccatggattg tcttttggaa
    10261 gttacgaact agtggagatt aaaccgccta aaggatatat attgagtcaa tcaaccattc
    10321 tctttgaagt tgaaaaaggc agttatgatc cgcttcatcc tataccattg gaaatcatca
    10381 acgaaccaaa agcaaatccg acgaataatg gaaaaacgcc agttcagatg cagacaaatg
    10441 gtccatcaaa tgaaaaaaat ggaggaagct ttccaaaaac aagcgaagag atgcttggag
    10501 gtttctcaat tttagggtta ttgctagtcc tttcaacagg gacagcttgg ttttataaaa
    10561 aacaacaaaa aggaaacaat aggagggaaa taaaatgaaa aacatcaaaa caaaaatatg
    10621 gacgttttta ggaactgcta tcatgctgct tcctttcgtt ctcggcctag gaactgcaga
    10681 ggtttctgca gccgtatcac ctacaccaga aaatgttacg gttaatttgc ataaattgaa
    10741 attcacttca gcaccagaaa atcaaataaa caacggaacg gaattgacat ttccgaatag
    10801 cgaaccgttg aacggcgttg aattcaacgt atatgacatc actgctacgt actatccaag
    10861 taaagacaca gcagttccag cagacgctac acccttcgca agtgtaacga cttctggtga
    10921 aggattagct aacctgactc ttccaggtaa atctgatggg aaagatgctg tatacgtctt
    10981 tgtagaaaca ccaaagcccg gtgtcgaaac gtcgccaaat atcgtgttaa gcttacccta
    11041 gaccctgaca aacctttaga cacgattcac ctatatgcta aaaatgtcat caaagaaagc
    11101 aatatcaaaa tccagaaagc tgggaacttt tcaggggtag ataagttagc aggggcgcaa
    11161 ttccagatca aaaattcaga gaatcaattc gtgaccggtt ttgatgaaaa cactggtgtc
    11221 gctctctatg atcctgctga gtcagcgttg acattcacaa ctgatactga aggatccatc
    11281 agtgtcaatg gattaataga cggcaattat actttagttg aaacaaaagc accagatggt
    11341 tatcaattaa ccaatccaca aaaagaaact acttttatag taaaaaacgg aaaattgcaa
    11401 caaacaaccg gtatcatcca tggcgcagat gaagccaatc ttgggaatcc aaacggtgta
    11461 gtcaatcggg aaaatacaac tacggataac acgattactg tagaaaatgt ccaaaactac
    11521 ggtgatttcc acttcatcaa acaagatgtg aacacgcaag aaaaattagc cgatgcagaa
    11581 ttttctattc taagagcaaa agatccaact gttaaattaa tgaaaaaaat agcaaacctt
    11641 gcctcaggag aatacccata tgcttggtca gatgaagtca caggttctga ctgggaagaa
    11701 gtgaaattga tttctgattc agaagggaaa ttttcaatcg aagggctgaa acaaggggca
    11761 tactgccttc aagaaacaaa agcaccagaa ggctacgcat tacctaagaa tgaagctgcg
    11821 tacactgaat ttacagtaga tgatgatcca acaacttctg atgaaacaac gatcgataac
    11881 caacctaaag gaatcttgcc ttcaactggt ggtatgggga tcatcgtatt catcttggta
    11941 ggtactgctt tagttggagg agcagtgatc tatttcaaaa aacgccatca agaaacagaa
    12001 gcttaaacta ttggaaacaa aaatcatacg ataaataaac gatagtgatt caaatgggga
    12061 gaaaggttgt gacaagtggt cggtctcttg gtattcgctc aacaaacggc aaaataggag
    12121 gaactatttt gccgtttgct tcgctaatac tcaggtcaag gcgctgattt cacaacctct
    12181 tcatatacat attagaaaat aggtggacat gatgaataaa agcagaataa gaatactgga
    12241 catcttcatg gcaatcattt tagtcgttgg gatcgggatc ttttcttatc cttttgtaga
    12301 agattcactg aatgattttc ttgcccagca aatgatcatc cattatcaaa agcaagcatc
    12361 gaataagaat tcagaggaaa taaaaaaaca gcaggaaaaa atgacaaaga aaaaccaaca
    12421 gttggcagag caaaacgtat caccagggat aggcagtttc aaccaagcag ttgatgcaaa
    12481 agcattaaaa gatttaccta gtaatgcctt ttttatggaa catatgcttg gagtcatcga
    12541 aatcccaaaa atcaatgtca gcttacctat attcgatcag acaacggaaa tatttttgca
    12601 aaaaggaacg tcattattgg aagaaagttc ttatccgact ggcgggaaaa acacccatgc
    12661 cgttctttct gggcatcgtg gactcccaga agcaaaatta tttacagatt tgcctaagtt
    12721 gaaaaaagga gaccagtttt tcatccagat caatggaaag acattggcgt atcaagtaga
    12781 aaaaatccaa gtggtcttac ctgatgaagt agattctttg ggtatccaaa aaggcagaga
    12841 tctcgtgact ttgcttacct gtactcctta tatggtcaat acacatcgac tgcttgtgac
    12901 ggggcatcgt attccttatc aagcaaaaga ggcaaaaaaa gcgatccaag gaattgatca
    12961 atggaaaaaa tggaaatttt ttatttggtt tatcggtata ctacttggat caatcggctt
    13021 agtttggcta ctcatcgctt atttagattc tttggccatc gctaagcgaa actatccttt
    13081 atctttttat gtaaagaaca caaacggacg accaatcgaa ggaatggtct tttctgtaaa
    13141 aacgctcaac ggcaaacact atattactag agaaaaagtt ccatttgtga aagcttctga
    13201 tgaatacgga ctagtgagat tttctgattt aaaaggcagg aactatcgct tgcaacatga
    13261 ggagctcctg cttaaaatcc atgtaaaaca taaacattcg aaacagtttt caatgaaatt
    13321 gaaaaaagga agatataaac tgcgaaaaga aaaagaggtg tactatttga ttgagaaaga
    13381 atgaatgaag atgtccaaaa aagcagaggg aacagactaa atgaaaaaag agagaagctg
    13441 acgaaatgga cgtgtaaact gttatttttg gtttactatc tggaacgcca tcttctattt
    13501 ttttatgttt cagaagaatt ttattattat agtataactt ttgaattagt ttccataaca
    13561 tttggagcaa ggggttaaac cacgagcttg agcttcgctt aaagtggcag gggtatacgt
    13621 accatttcca catttatgtg tatggtattt tgaaccggtc ggtgtgacat agacagtttg
    13681 ttcattattt tgctcaggag ctgcggcttg tgcctgctga gaggcctgtt cagctgctga
    13741 ttgctgtcgc gcctgttctg cttgttgctg tgcgattgct tcttgcctcg cctgttctgc
    13801 ttgttgctgt gcgattgctt cttgttgcgc ttgttctgct tgttgtcgtg caatttcttc
    13861 ttgctttttc cgttcttctt ctgcttcttt tgctttgatt gtggaatcaa ttgcagcaag
    13921 acggcttgtc aactcttgtt tgttttcagg tagcgcttgg atagctgctg aagcagttgc
    13981 atagttttct tttgtaggat ttgcttcagc ttgtgacaaa gcagattccg cttctgtcac
    14041 tttttttcta gtgcagcttt ttccgctttt tgtttctctt cttttttctt ttcaatttgt
    14101 tcttgttctt tcgatgaatc tgccgtactt tcggagatcg tggtactgct gctggaagcc
    14161 tgatctgaac ttgtgctttg agtagtagac gtagtagcac cacacccact caacaaactt
    14221 cctgcgacca aaaacaatgc aactaatttt ttcgctaaat ttcctcctta agtaaaaaac
    14281 aaccgttttc aataaatgtc cttttcaatt gtttacttac ttgttataat aattgtgtta
    14341 tgaatagtga ttgaggaaac ttaatctaat cccgtgttga tacacgggat tttttgctat
    14401 agttctgtta ttaagtatgc aataataaaa tatgtacgtt attttattat tagattattg
    14461 acatttatat aataaacgat taaatgattc atatcaatag aatacttagg aaaaaaagag
    14521 aaataataac aggtttatgc actattgacg gtgtgacaat atgatatttg tatccgtttt
    14581 ttttcttgta tgtagtatag taaagtagat acaggaggag aaaatatgca gactttttta
    14641 acaaaagttt ctcaaaagaa aattaaaatg attcatttac tgcttgaaac agaacgttgg
    14701 tgttcaatag aagaactaca aaacgaattg aaggtatcaa gtaagtctat cttgaattat
    14761 ctaacagatt tagaagaatt gttccaaaaa tatcctgata aggtcatttt gaagaatgag
    14821 aataatcggc gcttttctat ggaaaagcag gagaacttcc caatctacat tatttatctt
    14881 catttctaca gaaaatcata taattatcat ttgattgaat tcatgtatca acatcctgag
    14941 aaaaatttgg aagattatgc agacgctcaa tatacgagtg tatccacagt ttttcgttat
    15001 gctaagttat tagtaaagta ctttgaaatg acggacgtca aagaatagtc gccgccagat
    15061 agaaggaaat tagcagttga aaaagctgcc ctgcatcaag taatacgacc agccaaacgg
    15121 aaacagtgtc tcatgttttt gatttggctg gtcttattac cgcagatgca agcttttgcc
    15181 acacctcttt cttgtcattt acagactcaa ctatcaatgc tgcaagcttt cccttaatgc
    15241 tgtaacacca gtaagagttg tcaagaccag cagactgact aggtaaatac cgctcaaaaa
    15301 caagattacg gcagttaaac tataatgatc catcaagaaa ccgatgacga tagaagaaaa
    15361 accaccaaca gctcgtccaa ggttcataat gaaattattc gccgtcgcgc ggatttctgt
    15421 tggatacaaa ctactaatca ctgcgccata tccaccatac atgccattga taaagtagcc
    15481 gactatcaca gcggctgcta ccaaactcca ctggctatgg gctaacacaa gtagatagac
    15541 agataaggat gaacaaatta aaaacaaaga aaaggctagt ttaggcccca atttatccat
    15601 gattatgcca aaagtcatca tccccaaaga catgcctaaa atagtactga ccatccacaa
    15661 tgaagaccca gaaaccgaaa gccctaatcg attttgcata attgaaggca gccaattcat
    15721 taacccaaaa tagccggcaa tttggacagt taccatgata ctaaggccac atgtttgcca
    15781 agcaagatgt tttgattcaa acagtctgct aagtttggtt ttcttttcgg cagattgtac
    15841 agatttgaat tcctttggtt cagtcaagcc gcgacgaatg aacaagacca tcaacaccgg
    15901 cagtacacct acgacataca acatcttcca accaaacgtt ggaatgatca cagcagctaa
    15961 aatcgcagcc aaagccgcac cgacctgcgc accaatacca gcaaccgaag aagctcgtcc
    16021 aatttgtttt tttgaaaaac tttcggaaat caaggacata cacgccccgt attctcctcc
    16081 cgctcctaca cctgcgataa agcgaaagag ataaaccaag tagatgtttg aagcaaaata
    16141 catcaataaa gaagcaatcg aaaacagaag gaccgtttga gaaaatactt tcacacgtcc
    16201 atatttatca gctatgatgc caaaaaaaaa tacctccaat gagcatcccc aaattagtga
    16261 tcgttgaaat gaaacccgct tgcgtaccac taatatgaaa agaagtaatc attgaagata
    16321 atgaaaaagc taaaaacata atatccatat tctctaaagc aattcccgcc gatgtcgata
    16381 ataatacctt tcgctgatac gttgtcattt ttatacccct tttcctaaaa tttccctaaa
    16441 ttgttaaaag tatatccaaa aaagaataaa aaaacaagac ttttttattt gaaataatac
    16501 acaaacaacc gatatagata atccagctta gttttaatag aaatgagtat tgacttggag
    16561 tacacttaaa gttgtagaat aaataaaata cataagtaaa gtggcaagaa attaagagaa
    16621 agagtgatca gatgaatatc aaacaagtca gtgaagaaaa gggcatttcg gccgatacat
    16681 tgcgctatta tgaacgaatc gggttgattc caccagttaa tcggacaaaa ggcggaatcc
    16741 gtgattatac agaagaagac ctaaaatggg tcgactttac gatctgtatg cgaaatgccg
    16801 gattatcgat cgagtctttg attgaatata ttcgcctatc tagcgaagga gaagcaactg
    16861 tatttgagcg gcgtaagctt ttgattgaag aaagtgaact tttgacgaaa gaaattgctg
    16921 aaatgcaaga gtgtttggaa cgtttacaag gaaaaatcaa gaaatatgat cgtgtgttat
    16981 ctagaaataa aatggaatgt atcaagtaaa aaaatcaagg agcatcgcta tggagttacg
    17041 cttactaaaa tatttttgga caattgcaga agaaggaaca atctctcaag ccgcagaagt
    17101 tctacatctt acacaaccaa ccttaagccg acaactgaag gagttagaag aggaactagg
    17161 aacagaactg tttgtacgcg aaaaccgcaa aatgatcttg acagaagcag gttattttct
    17221 aaaaaagccg agcagaagaa attttagatt taacgaatca aaccactcaa gaattcgaga
    17281 acagaaaaaa acaattattc agcggccgat ttacgatcgg ctgtgtggaa gctgataatt
    17341 cgaatacatt ggcactatta ctagaaaagt tgatccgtga ttatcccagc gttcaatttc
    17401 atatccacag cggaacaagt gatgatatca cggagtgact agaaaaaagc ctgttagatt
    17461 tagcgatttt gctagatcca gtagatacag agaaatacga aacgattttg ttaccaagaa
    17521 aggaaaaatg gggattgctc attttagaaa attctgtttt agcaaaaaaa gacgtgataa
    17581 caaaagaaga ctttgcgaat gtacctcttt tgatctcaaa gcgtccagaa atacagcgaa
    17641 tgtttgctga ctggttaggc ataaaaaagg agcagttgct gatcacagga acgtacaatt
    17701 tgattttcaa tgtatttgcg ctaattgaga atcaggtagg tgctgcttta gtcatagaag
    17761 gtgctgtaag tcatctttca aagcgagtga cattcgttcc gcttgaccca atgattgaaa
    17821 cgaactgcat gctagtctgg aaaaagaatc ggattttatc acctgtagtc aatgaattga
    17881 ttcaccgttt caaagaagct tttgaacagt aataagtatc tctaatcgaa gaaatttcaa
    17941 tacaaaagcg agacgtatga cagtcttgct tttgtatttt ttcattttta aatcaaaaaa
    18001 aataaagaga tttacataac ttgttaaata agcgcttaca aacataagaa gaacggaaat
    18061 gaatcagcta taataaccgt tgttctggat tgtaatcatt acaaagaaat gaagattata
    18121 actttggagg tgttcgaaat gaaagaaaaa aagcaaaaat tcgttcaaaa aacatgccct
    18181 ttattcaatg gttgggtatg attctgctga ttgtattagc tttattgacg atcattcctt
    18241 ttttatggat ggtttctgct tcattcaaag caaataatga agtgttcacg attccgattc
    18301 aatggatccc taaaacttgg catttagaga attatctggt tatttggtct cgtattcctt
    18361 tagtgacatt ttttaagaat acagcttttt taagcgttgt gatcacgttg attcagttgt
    18421 ttacttctag ttttgctgct tacgggtttt ctaaactaaa ttttaaaggg cgcgatattt
    18481 tatttttagc atatatcggc acgattgctg tcccttggca gtcttatatg atcccgcagt
    18541 tcattatgat gagaaaagtg ggtttaacat atacgttgtg gtcgttggtg ctattacaag
    18601 cgttcaatgc ttttggtgtg ttcttgttga agcaatacta cagtagtatc ccagattctt
    18661 tatgtgagag tgcgcggatc gatggcttat cagagtgggg aatctatcgc aagatcattt
    18721 tacctcttac aaaaccggcg attgccagtt tgacgatcat cacattcgtt aatacatgga
    18781 acgattatat ggggccattc atttatttgt cttctacgga aaataaaaca atccagttag
    18841 ggttgaaaat gtttattggg ctatttgatg cagagtacgc attaatcatg gctgcctcag
    18901 ttgtttctgt tcttcctgtt gcttttatat ttttagcgat gcaaaaatat tttgtagaag
    18961 ggatcgcttc atcaggaatg aaggaatagg aggtgattat gaaagagcag cgttttgaca
    19021 aaaatgttta tatggaattc ttttattggt tgtatcaatc attggcaagt agcctttgtt
    19081 tttggtgtgt caatatcttg tatatagcag ctgcgttcct tttgtcgata gatggaagga
    19141 atgtcttttc ctttattttt tctttacttt ttattggtcc aggttgca
//
LOCUS       DACEOM010000061        19043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEOM010000061 DACEOM010000000
VERSION     DACEOM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.062"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            123..299
                     /locus_tag="IW781_002104"
     CDS             123..299
                     /locus_tag="IW781_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1183097.1"
                     /translation="MDYMTLKEASEKWGVTPRQINYLCAGGRIPGAVKMATIWLIPKD
                     AEKPADRRFKNTKK"
     gene            361..1044
                     /locus_tag="IW781_002105"
     CDS             361..1044
                     /locus_tag="IW781_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1183098.1"
                     /translation="MRILVVEDDRLLNNTLCYNLDTAGYAVDSALTKSAASNFLTKQD
                     YDLIVLDVNLPDGNGFDFCREVKERRPDTAVIFLTANDMESDMLKGYELGAEDYVTKP
                     FPMSVFQKKLSVVLGRLAKQSGGDCYEDGALSINFSEMTASLSGKPLVFTPLEYRLLK
                     IMTKNPQNVLTRQVLLEKLWDADGNFVDEHALTAAISRVRNKIETEGRQYIKTVYGMG
                     YMWIGGAPK"
     gene            1041..2072
                     /locus_tag="IW781_002106"
     CDS             1041..2072
                     /locus_tag="IW781_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1183099.1"
                     /translation="MNMRKLSINKACILLGTLLLLAVGAVCAAVYLLTQNITAVRCVF
                     LFSLFVLLCVIFFVALIRRKLVRFSDAFCGQMDDMLSGDMQPKQTVEEESLFYKINYR
                     LGRLYEVMQENKNSIAKERADLQELIADISHQVKTPIANLKMINNTLLENEVPPQKQK
                     EFLTAQASQLDKLDFLMQAMIKTSRLETGVISLEQKQQPVYDTLAAALGGILLNAEKK
                     QIDVQVECPEHLDARHDRKWTSEALFNILDNAVKYTPAGGQIRVSVEGWEMYVKIDIA
                     DTGIGISEQHQGTIFKRFYREDAVHDVDGIGIGLYLAREIVTLQGGYIRVASEVGKGS
                     TFSVFLLRK"
     gene            2217..2882
                     /locus_tag="IW781_002107"
     CDS             2217..2882
                     /locus_tag="IW781_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183100.1"
                     /translation="MSILQTTELKKYYGAEPNITKALDGVTLSIERGEFVAIIGTSGS
                     GKSTLLNMMGGLDVPTSGQVIVDGKELSTLNDEALTIFRRRKIGFIFQNYNLVPVLNV
                     YENIVLPVELDGDKVDKKFMDEVVRMLALEDKLNNMPNNLSGGQQQRVAIARALMSKP
                     AIVLADEPTGNLDSKTSADVLGLLKVTSQKFHQTLVMITHNTEIAQLADRIIRIEDGK
                     ISE"
     gene            2897..5470
                     /locus_tag="IW781_002108"
     CDS             2897..5470
                     /locus_tag="IW781_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1183101.1"
                     /translation="MNDILFGNTNRPVIKKLAGRYFKSSKSRNVIAVIAIILTSILFT
                     TIFTLGSGLLDTIQDHNIRKAGGDGHAALSYISDDIFDEIKDNELIDRISYTKCVSYR
                     LHNAGLEKWRAEMWYMDDTALEFGRYEPTTGRTPEAENEIMVDTETLKALGVPAELGA
                     TVPLEYEIKGETYTTDFVLCGFWETDSLSNIGRLIVSKAFIDVNSDLLAYTYPVDNDY
                     SGVVVAYIMFKGNGAIEPKLNQLLSEAGYTCDTMGGIRGDENYINAGVNPAYQGSSLL
                     ENPFMLVSGIVGVLLIMMTGYLIIYNIFQISVIQDIQSYGQLKTLGTTRRQIKKLISK
                     QVIRLSCIGIPIGLLIGFFIGRSLVPFLMNGTAYTAAAGIKVSVNPLIFIGSAIFAWI
                     TVYISVRKPAKIAGTVSPVEAIRYTENDTSAFKGKKAITKKSTHGAQIYRMALANLGR
                     NKKRTILVIVSMTLSLVLFNTVFTLSSGFDIDKYVSKFLNKDFIISSADYFQYRFDRS
                     DAELSQTFIHAVQQEDAYADGGELSTVRVLEEFFSVESETIVNHNKDKAGNPHVSLYG
                     ADDFLLKSMEVIEGEIDWEALHSGKYVLYGLQSDDNGNIIQDQSVQVGDVLRFHHSTA
                     DGLNSTMDATVDLTVMAKVRINNSVDTHRQTGGTNFYMPTENFKPLCTDPHVVNFSFN
                     VKPGQEMAMEDFLTSYTENIEPGMDYESKQTYVNSFHDLTSMIITIGGALSVIIGLIG
                     ITNFINSMLTSIITRKKEFAMLQSIGMTGKQLKSMLACEGLYYAIGTILASAFFGTLF
                     SLIIVQGIANSIWFFTYRFVIWPMLVIYPFLLAVTIIIPSILYREIAKTSIIERLRTS
                     A"
     gene            6057..6491
                     /locus_tag="IW781_002109"
     CDS             6057..6491
                     /locus_tag="IW781_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1183102.1"
                     /translation="MKEYTPDYVKCCQFDHYCKLVLYHEAIDYLREMQRYRKYQISLE
                     DISLAQWDKFSTRDEYPSDSFVFSAFGYALHIRDELVAGAFVKLPEQEQQILILHYVA
                     TMADTNIGRLMGMSRSAVQRHRTKALNELRKRLEDDGGRKAK"
     gene            6488..6727
                     /locus_tag="IW781_002110"
     CDS             6488..6727
                     /locus_tag="IW781_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1183103.1"
                     /translation="MKQPTFYGRELLPLSVIEAARAGAPLAVESVLRYYDGYMNKLCT
                     RTLYDLNGLPHVRVDEYMKDRLQNKLIQTILKSSL"
     gene            complement(6884..7255)
                     /locus_tag="IW781_002111"
     CDS             complement(6884..7255)
                     /locus_tag="IW781_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183104.1"
                     /translation="MNIPRMSYPQYRKARRLTHECCNYCNGNCLLLDDGEECVCVQSI
                     SYSLLCRWFKVAVLPLDAALCAEITKSRDEIKRCAVCGAAFTPNSNRAKYCPDCAVQV
                     RRKKEAERQRKRYLLSTHLGR"
     gene            complement(7252..8586)
                     /locus_tag="IW781_002112"
     CDS             complement(7252..8586)
                     /locus_tag="IW781_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1183105.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDEKLFVSSYGCSYETADI
                     EFQMLLDQAYQKGNNLAHHLIQAFEPGETTAEQAHEIGRQLADEVLQGKYPYVITTHI
                     DKGHLHNHIIFCAVDMANQRKYISNRQSYAFIRRTSDRLCKEHGLSVVKPGKDKGKTY
                     AEWDAQKKGKSWKAKLKIAIDAAIPQAKDFDGFLRLMETQGYEVKQGKFISFRAPGQE
                     RFTRCKTLGEDYTEERITRRIKGIAIDRGPLRRSDGEISLRIALEDSIKAQQSAGYAR
                     WAKLHNLKQAANSLNFITEHQIDSYEGLESRLAEISAANDAAASALKDAERRLGDMAL
                     LIKNLSAYKQLRPVALELRNAKDKAAFRRQHESQLILYEAAAKTLKEAGVTKLPNLYA
                     LKTEYKKLDAERERLSTQYSEAKQKLKEYGIVKQNVDSILRTAPGKEHTQER"
     gene            complement(8547..8894)
                     /gene="mobC"
                     /locus_tag="IW781_002113"
     CDS             complement(8547..8894)
                     /gene="mobC"
                     /locus_tag="IW781_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007036726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1183106.1"
                     /translation="MNGGAKVANRKRKFVLRVPVTPEERALIQQKMAQLGTKNFSAYA
                     RKMLIDGYIVHIDTGPVRAQTAELQKIGVNINQIARRINSTGTVYAQDLEDIKGALAQ
                     IWQLQRSILLSQR"
     gene            complement(9105..9473)
                     /locus_tag="IW781_002114"
     CDS             complement(9105..9473)
                     /locus_tag="IW781_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183107.1"
                     /translation="MANIKDCPGFETFGADVKAARKVKQLSRKTLAEQVNIDWRYLAN
                     IENDGTIPSLPVIIQFIKICGLPVERYFNPEIMREESAQRQRVSHKLKLCPEEFLPII
                     EGAIDGAIQIQQKNDETEGA"
     gene            9694..10386
                     /locus_tag="IW781_002115"
     CDS             9694..10386
                     /locus_tag="IW781_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1183108.1"
                     /translation="MKQILIVEDDSFLNKMLAYNLTADGYGVTSTLNARTAADAIRQR
                     EFDLVLLDINLPDGNGFELCRLIKPQHPDTIVIFLTANDQESDQIRGYEVGAVDYITK
                     PFVIGALQRKIKAMFAMLEHHKPAKDIYDDGRLFLDFSEQTASLNGKPLTLSPMEYKM
                     LNLFRKNPRQVLTRGQLLEKLWDIDEKYVDEHTLTTSISRIRSKIESDGGAPYIKTVY
                     GMGYQWTGGEAK"
     gene            10383..11414
                     /locus_tag="IW781_002116"
     CDS             10383..11414
                     /locus_tag="IW781_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1183109.1"
                     /translation="MKCKNLSVKRLFGRVAIGLVLSMSGITIALFFVTKQIAVLLTGG
                     ALLLCALVGIFVLTQAFGKRLSQFTADLCQTLDHMIAGNEAPQRPEDSETQLARIGHR
                     LARLYQIMQENRRKVDEERQELQTLVSDISHQVKTPVSNLKMATDTLLEKPMTETERT
                     DFIRGIRSQTDKLDFLFQALVKTSRLETGVIQLDKKTGRLFDTVAQAMSGIVYAAEKK
                     EIAVSVDCPEDLTVSHDSKWTSEALFNLLDNAVKYTSAGGKIAVSVVLWEMYVEIKVI
                     DTGKGISESNQAAIFRRFYREEEVHEQQGVGIGLYLAREIVTRQGGYIKVVSEPGKGS
                     EFSIMLPTK"
     gene            11592..12257
                     /locus_tag="IW781_002117"
     CDS             11592..12257
                     /locus_tag="IW781_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183110.1"
                     /translation="MSVLQTIDLKKYYGTKPNITHALDGVNFSVEDGEFVAIVGTSGS
                     GKSTLLHMMGGLDTPTSGNVIVRDKELSKMNDEQLTIFRRRNIGFIFQNYNLVPILNV
                     YENIVLPVELDGDTVDKKFLNEIVHLLGLEDKLKNMPNNLSGGQQQRVAIARALITKP
                     AIVLADEPTGNLDSKTSAEVLGLIKRTSAEFRQTVVMITHNDDIARLADRIVRIEDGK
                     IVE"
     gene            12272..14704
                     /locus_tag="IW781_002118"
     CDS             12272..14704
                     /locus_tag="IW781_002118"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1183111.1"
                     /translation="MTWPFENDTSAVVKKLTKRSILADKKSNLLLIVTIALSVCMVLS
                     LALSTKGIAEAQKDSYRDKAQITVVAPTEEQLAQIKNNSNVQWIGEYLFLGSSYQDNI
                     ELGISYANADYIEKERGLVYSGSLPKDDNEILLEQDYIDHYAPNCQVGGTIVLDLTGT
                     GETTEYRISGIIANSENTLGEAYYDVFVSKTYAQMLLSKTLDRDFQITAYTRLNTSAI
                     SSGEILSFAYDITRPVGIDDEQVFLTDYFAVMSGVIKDGLYISIPLITFITSVLAAVV
                     IYGIFYTVVVKNVQTIGQLRTIGMTKRQIKKMVRRNSLQFSAKGIFLGICLGTVIGFC
                     VCPSGFRIKTALMYGIFATIISAFAITIAVRKPIKIAYSTSPIAGMHYLPYEVKKKNS
                     HKLHRKLTSANLAKINISRNRGKAIFTFLTLSISGIIFLTSALVAGSIDAEKQARFLY
                     FPDGEIQIQLRNAAQSTFTANGEYNYSTFLQLGNNPLENVDFIEKIEKMDGVEKVTPH
                     NAIMTTIGLPVGMGNWLYRSNYAPTLTQEQFSIVSDLLTAGSIDYTEMTKTNGIFVED
                     KYASVGETLTLQLRGIDGNELSLDVPVVGTYDGSKLMSVYPLVPGNPNFLMTYDTVKN
                     LTGVSAQTGILSIDVTKEKYDAVLSQINEIAQSGDEIIVFDIAQNISGIKTEYNASIK
                     NLYLVSVILFLFGGISLTNTLLVDFKNRKHEFALLKAIGTTQKQLNNMLNREIMTYLA
                     GSFSIALLGSTIASIIVCKRLDSINHCIRFSFPWLFLFAFVALLIIIYFIFSFYVSRE
                     LKNTDVIKLIRE"
     gene            14726..14983
                     /locus_tag="IW781_002119"
     CDS             14726..14983
                     /locus_tag="IW781_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1183112.1"
                     /translation="MEKETMGTVISVTKQWWLKVNRKPARAHAMDGAAFPHTIKVKYT
                     IDGKDYICRKWIGAGNKIPDKGTTIKVTYYEDKPSKARIEL"
     gene            15085..15276
                     /locus_tag="IW781_002120"
     CDS             15085..15276
                     /locus_tag="IW781_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183113.1"
                     /translation="MSKSENNHDNSDFQMLKEEISESEYQNAQYEAEKKKHMSYWVMI
                     IAPFIMILVIFIFYYCFYR"
     gene            15812..16243
                     /locus_tag="IW781_002121"
     CDS             15812..16243
                     /locus_tag="IW781_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1183114.1"
                     /translation="MEAIPRKYGEWGQFDRYCKLVLYHEAIDYLREMKCRRDREVSLD
                     AVSPADWDKLSTVDRYSCDSFTFSSHGYDLHIDNELVAEAFASLPKDEQSILILRFVL
                     DLTDEEIGRLMGMSRSAVQRHRTSTLKDLRIKLMAFMPEGG"
     gene            16250..16492
                     /locus_tag="IW781_002122"
     CDS             16250..16492
                     /locus_tag="IW781_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1183115.1"
                     /translation="MKQSSYKGKSPLPDFVIDAACAGNAEAVERVLQHYDGYINKLCT
                     RTLYDGSGQPHICIDEYMKRRLQIKLIHSIVSPIGD"
     gene            complement(16473..16658)
                     /locus_tag="IW781_002123"
     CDS             complement(16473..16658)
                     /locus_tag="IW781_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183116.1"
                     /translation="MAHLPLGSAQTECIRLPYDAPPLSCGRICQGAKGPPGKQASGER
                     ESRLSMAGALTVSRQWD"
     gene            16926..17129
                     /locus_tag="IW781_002124"
     CDS             16926..17129
                     /locus_tag="IW781_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ1183117.1"
                     /translation="MSNNDVPIWEKYTLTIEEASKYFRIGENKLRRLAEENPTAGWAI
                     LNGNRIQIKRKQFEKIIDSLDTI"
     gene            17209..18486
                     /locus_tag="IW781_002125"
     CDS             17209..18486
                     /locus_tag="IW781_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1183118.1"
                     /translation="MPRKERDEKRRDNKGRLLKSGESQRSDGRYAYKYIDTFGEPQFV
                     YSWKLVPTDKVPAGKRPCLSLREKIKQIQKDLDDGIDTIGKKMTVCQLYEKYTRQRGN
                     VRKGTQKSRQQLMKLLSEDKLGAASIDSVKLSDAKEWALRMQEKGVAYNTICNSKRSL
                     KAMFYMAVQDDCLRKNPFDFQINEVINDDTVPKVPLTPTQENELLDFMQNDPVYAEYY
                     DEVVILLETGLRISELCGLTPADLNFEKRFVNVDHQLLRSTEDGYYIEAPKTESGFRQ
                     VPMSAAAYEAFERVLKKRRDGRCIEVDGYKDFLFLNRDGLPKTAVNYDAMFKCLAKKY
                     NKCHKEPLPDVMTPHTMRHTFCTRMANAGMNPKALQYIMGHANIVMTLNYYAHATFHS
                     AQEEMERLQAKAKTTAEAKPELAAESTEESKAA"
     gene            18574..18795
                     /locus_tag="IW781_002126"
     CDS             18574..18795
                     /locus_tag="IW781_002126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183119.1"
                     /translation="MKSAGKARNNGLYRHIRTSKEIIRNLLNDLAKDNIKKYKKGNIF
                     HIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT         5183 a         4203 c         4695 g         4962 t
ORIGIN      
        1 tgggcctctt tcttccggta catggctttt tctggatatg acttgcactt tattattcat
       61 atttcctgcc ttgtattctt cgagcagaag aaatacaata aaccatattg gagggaaact
      121 atatggatta tatgacgctg aaagaagcaa gtgaaaaatg gggtgttacc ccaagacaga
      181 tcaattatct gtgtgccggt gggcgtatcc caggtgctgt gaagatggct accatttggc
      241 tgattcccaa ggatgcggag aaaccggcag accgtcgttt caaaaataca aaaaaataat
      301 cctctcgtga ggtttctgtg acatttggat gctacactaa gaatgaagga gtgtgataca
      361 atgcgaattt tagtagtcga ggatgaccga cttttgaata ataccttgtg ctataacctg
      421 gatactgcgg gctatgccgt ggactctgca ttgacaaaat cagcagccag caactttctg
      481 accaagcagg actatgacct gatcgtgctg gatgtaaacc tgccggatgg gaatggcttt
      541 gatttctgcc gtgaggtaaa ggagcgccgg ccggatacgg cagtgatctt cctgaccgca
      601 aatgatatgg agagcgatat gctcaaaggc tatgagctgg gcgcagagga ctatgtgacc
      661 aagccatttc ctatgagcgt ctttcaaaaa aagttgtctg tggtgctggg gcggctggcg
      721 aaacagtctg gcggcgattg ctatgaggat ggcgcactat ccatcaattt ctcggagatg
      781 actgcttccc tgtccggtaa gccgctggtg ttcactccat tggaataccg gctgttgaaa
      841 atcatgacca aaaacccgca gaatgttttg accagacagg tgctgcttga aaagctgtgg
      901 gatgccgatg ggaactttgt ggatgaacac gccctgactg cggccatcag ccgggtgcgg
      961 aacaaaattg aaacggaggg ccgccagtac atcaagacgg tgtacggtat gggctatatg
     1021 tggattggag gggcaccgaa atgaatatgc gaaaactctc catcaacaaa gcctgtatcc
     1081 ttttgggaac gcttttgctg ctggcggttg gtgcagtctg cgccgctgtg tatctgctga
     1141 cccagaatat aactgctgtc cggtgtgtgt tcctgttcag tctctttgta ctgctctgcg
     1201 ttatcttctt tgtggctctg atccgccgga agctggtccg gttctccgat gccttttgtg
     1261 gtcagatgga tgatatgctc tccggggata tgcagcccaa gcagacggtt gaagaagaaa
     1321 gtctgttcta taaaatcaac taccggctgg ggcggctgta cgaggtcatg caggaaaata
     1381 aaaacagcat cgcaaaggaa cgggctgacc tgcaagagct gattgctgac atctcccatc
     1441 aggtcaagac cccgattgcc aacttgaaga tgatcaacaa cacactgctt gaaaacgagg
     1501 ttcccccgca gaagcaaaag gaatttctga cagcccaggc cagccagctt gataagctgg
     1561 acttcctcat gcaagccatg attaaaacct cccgcctgga aacgggagtc atttcgctgg
     1621 agcaaaaaca gcagccggtt tatgataccc ttgccgctgc cctgggcggg attttgctca
     1681 atgccgaaaa gaaacaaata gatgtgcagg tggaatgccc ggagcatttg gatgcgcgcc
     1741 acgatagaaa atggacaagc gaagccctgt ttaacattct ggataacgca gtgaagtaca
     1801 ccccggcagg cgggcagatc cgtgtgtcgg tggaaggctg ggaaatgtat gtgaagatcg
     1861 acattgccga caccggcatc ggcatttcag agcagcacca ggggacgatt ttcaagcggt
     1921 tttaccggga agatgcggtg catgatgtgg acggtattgg aatcggcctg tatctggccc
     1981 gtgagatcgt gaccttgcag ggcggctata tccgggtggc gtcggaagtt gggaaaggct
     2041 caaccttttc tgttttcctg ctccgaaagt agagcaagca cgccccgaaa aaatgaaatg
     2101 tcacagcttc gtgacatttg gggccgaatt tagcggaaat tttttgtggc tcgtgacatt
     2161 tctgtgaggt ttggtcgtta caatgggttt atcaaaaaga aaggatggtg ttctttatga
     2221 gcattttgca aacgactgaa ctgaaaaaat attatggcgc agagccgaat atcacaaaag
     2281 cactggatgg tgttaccctc tctattgagc gtggtgagtt tgttgcaatt attggaactt
     2341 ctggaagcgg aaaatccact ttactgaata tgatgggcgg tctggatgtg ccaacttccg
     2401 gtcaggtaat cgtggatggc aaggaacttt ccaccctaaa cgatgaggca ctgactattt
     2461 tccgtagacg aaaaatcggc tttatcttcc agaattacaa tctggttccg gtgctgaatg
     2521 tctatgaaaa tattgtgttg cctgtggaac tggacggcga taaggttgac aagaagttta
     2581 tggatgaagt agtgcggatg ctggcactgg aggataagct gaacaatatg cccaacaacc
     2641 tttccggcgg tcagcaacag cgtgtggcga ttgcccgcgc tctgatgtca aagcccgcta
     2701 tcgttctggc cgatgaaccc accggcaatc tggacagcaa aaccagcgcc gatgtattgg
     2761 gacttttgaa ggtaaccagc caaaaattcc accaaaccct tgtgatgatt acccacaata
     2821 ccgagattgc ccagcttgcc gaccgcatta tccgcattga ggatggcaag atttccgagt
     2881 aagggggcga aagaaaatga atgatatttt atttggaaac acaaatcgcc ctgttatcaa
     2941 gaaattggct gggagatatt ttaagtccag taaatccaga aatgtgattg ctgttattgc
     3001 aattattttg acaagcattt tattcacaac cattttcacg ctgggttctg gtttgctgga
     3061 caccattcaa gatcacaata ttcgtaaggc gggcggtgat ggtcatgccg ctctctccta
     3121 catcagtgat gacatttttg acgaaataaa agataacgaa ctgattgacc gtatttccta
     3181 tacaaaatgt gtttcctatc gtctgcataa tgcaggactt gaaaaatggc gggcagaaat
     3241 gtggtatatg gatgatacag cattggagtt tggccgttac gaacctacta cgggacgcac
     3301 gccagaagca gaaaatgaaa ttatggtcga tacagaaacg ctaaaagctt tgggcgttcc
     3361 tgccgaattg ggagcaacgg tacctcttga atatgaaata aaaggggaaa cttacaccac
     3421 cgactttgta ctgtgtggat tttgggaaac ggacagttta agcaatattg ggcggctgat
     3481 tgtttccaag gcttttattg atgtgaactc tgatctgttg gcctatacct atcctgttga
     3541 taatgattac tcaggcgtag tagtcgctta tatcatgttc aagggaaatg gtgcgattga
     3601 accgaaatta aaccaacttt tgagcgaagc tggttatacc tgcgatacaa tggggggtat
     3661 tcgaggtgat gaaaattata taaatgctgg tgttaatcct gcttatcaag ggagcagtct
     3721 gttggaaaat ccgtttatgc tggtttctgg tattgtaggt gttctactca ttatgatgac
     3781 tgggtatctg attatttata atatttttca gatttctgta atccaagaca ttcaatccta
     3841 tgggcaatta aaaacactgg gaactacaag acggcagatc aaaaaactaa ttagcaagca
     3901 agttatacgg ctatcttgta ttggcatccc gattgggctg ttgataggtt ttttcattgg
     3961 ccggtcatta gtaccatttt tgatgaatgg aaccgcatat acggctgctg ctggtataaa
     4021 agttagcgta aacccactta tttttattgg ctctgctatt tttgcgtgga ttacagtcta
     4081 tatcagtgtt cgtaaacctg caaagatcgc tggaactgtt tcccctgttg aggcgatccg
     4141 ctatacggaa aatgacactt ctgcctttaa gggcaaaaaa gcaattacaa aaaaatcaac
     4201 acacggcgca cagatttatc gaatggcact ggcaaacctg gggcgaaata aaaaacgcac
     4261 tatcttagta attgtatcaa tgaccttgag tttagtgctg ttcaacactg tattcacact
     4321 atccagcgga tttgatattg ataagtatgt aagtaaattt ctgaacaagg attttatcat
     4381 ttcatcggca gactatttcc agtatcgttt tgatcgaagt gatgctgaat tgtcgcaaac
     4441 atttattcat gccgttcaac aggaagatgc ctacgcggat ggcggagagt tgtctacggt
     4501 tcgagtattg gaagaatttt tctctgtgga aagtgaaaca atcgtaaacc ataacaagga
     4561 taaggcagga aatccccatg tttctcttta tggtgcagat gatttcctgc taaaatcaat
     4621 ggaagtaatt gagggggaaa ttgattggga agctctgcac tctggaaaat atgtacttta
     4681 tggtctgcaa agtgatgata acgggaatat tatccaagat caatcggtac aagtcggtga
     4741 tgtcctgcgt ttccatcatt cgacggcaga tggactcaat agtacaatgg atgctacggt
     4801 tgatttaacg gtgatggcaa aagtccgaat caataacagc gttgataccc accggcaaac
     4861 aggcggaaca aatttctata tgccaacaga gaactttaag ccactttgta ccgatcccca
     4921 tgtggtcaac ttctcattta atgtgaagcc ggggcaagag atggctatgg aagatttttt
     4981 aacttcctat acagaaaata ttgagccggg aatggattat gagtccaagc aaacctatgt
     5041 caactccttc catgatctaa cttcgatgat cattacgatt ggcggcgcct tgagcgtcat
     5101 tattggcttg attggtatta ctaattttat caattccatg cttaccagta ttatcacacg
     5161 aaaaaaagag ttcgctatgc tacaaagcat tggcatgact ggaaagcagt taaaatccat
     5221 gctggcttgt gagggattat actatgcaat aggaacaatt ctggcttctg ctttcttcgg
     5281 cacactgttt tcgcttatca ttgtgcaggg aattgcaaac agcatttggt tcttcacata
     5341 tcgctttgtc atttggccga tgttagtgat ttacccgttc ttattggccg tgacgattat
     5401 aattccatcc attctatata gggaaatagc taaaacgagt attatagaac gcttgcggac
     5461 atctgcatag taaaaatgaa aagccaggga aaatttccct ggcttttcaa atgtcacaag
     5521 atggtgagat ttcagcttgt tattttgtcg aaatggcgta tgcccgtgac atttctgtga
     5581 ggattgcctg ttaaaataaa agaaaagtga aaaagttgcc gcacgatggc aaaagaaaaa
     5641 aagctatcgt acagcgacta tattgttatg cgctttgtaa cggtggcttt gagaaaatca
     5701 gagccgccgt ttccttgctc ataaactgac gacgccctga cgctggcgga atcggcggcc
     5761 tacggaggga gccgccatga cacaaacagc atttcgacat aatacaccgg gttacgcacc
     5821 atcaaaaaaa gccgtccccc ggcagcgggg acaagcggct atgagtatgt actataccat
     5881 gtaggaaaag aacatattgc tttttgtttc gcccagcggg tctttgacct gccgggcgaa
     5941 ttttgttatt tcctgcggtt cttcggtgcg ggctggagcc gccgcattgt tacagatgaa
     6001 aggagtggtg aaaacagctt tatcttctca ttctcccgca gatcgggggt gagaagatga
     6061 aagaatatac tcctgattat gtgaaatgtt gtcagttcga ccattactgc aagctggttc
     6121 tataccatga agcgattgac tacctgcggg aaatgcagag ataccgcaag taccaaatat
     6181 ccttagagga tatttccctg gctcaatggg acaagttctc cacacgggac gagtacccca
     6241 gcgacagctt tgtgttctcg gcctttggct atgccctaca tatccgggat gagctggtgg
     6301 ccggagcctt tgtcaagctg ccggagcagg agcagcaaat tttaattctg cattatgtcg
     6361 caactatggc agacaccaac attggccgtc ttatgggaat gtcccgcagc gccgttcaac
     6421 ggcaccgtac caaagccctg aatgaattgc ggaaacgatt ggaggacgac ggagggagga
     6481 aagcgaaatg aagcagccaa ctttctacgg gcgggaactg ttgcccttgt ctgtgatcga
     6541 ggcggctcgc gccggtgctc cgctggccgt ggaaagtgtc ttgcgatatt atgacggcta
     6601 catgaataaa ctgtgtaccc ggacactcta cgatctgaac ggtttgcctc atgtgagggt
     6661 ggatgaatac atgaaagacc gcctgcaaaa caagctgatc caaactattc taaagagcag
     6721 cctatgagaa aacgccgggc gcaggggctg tcaaagcctc tgcgtccggc acttcttttt
     6781 ttgtccattt tcccgcattt agaaccccgt tttgagggga acaggataac tttatccacc
     6841 tgcctgccga ccatgcggaa aaacccttga tttccgtgga cttttaccgc cctaaatgcg
     6901 tagacaggag gtatcttttc cgctggcgct ccgcctcttt cttccggcgc acctgcaccg
     6961 cacaatccgg gcagtatttg gcccgattgg agttgggcgt aaacgccgcc ccgcagacgg
     7021 cgcagcgttt gatctcgtcc cggcttttgg tgatctcggc gcacagggcc gcgtccagcg
     7081 gcaggacggc caccttgaac caccggcaca acagcgaata ggaaatactc tgcacacaga
     7141 cacattcctc cccatcgtcc agtaggaggc agtttccgtt acagtagttg cagcactcat
     7201 gggtgagcct gcgggccttg cggtattggg ggtaactcat tctcggtatg ttcatcgttc
     7261 ctgcgtatgc tcctttcccg gcgctgtccg caaaatgctg tctacattct gtttgacaat
     7321 gccgtattct ttcaatttct gtttcgcttc gctgtactgt gtggacagcc gttcccgttc
     7381 tgcgtccagc tttttatact cggttttgag ggcgtacagg ttggggagct ttgtgacccc
     7441 ggcctctttg agcgtctttg ccgccgcctc atacaaaatc agttggcttt catgctgccg
     7501 ccggaacgcg gccttgtctt tggcatttcg cagttccagt gccacaggcc ggagctgctt
     7561 gtaggcagag aggtttttta tcagcagcgc catatcccca aggcggcgtt ctgcgtcttt
     7621 cagggcggag gccgccgcgt cgtttgccgc gctgatctcg gcaagcctgc tttccaagcc
     7681 ctcgtagctg tcgatctgat gttctgtgat gaagtttaag gaattggcag cctgcttcaa
     7741 gttgtgcagc ttcgcccacc gcgcatagcc cgccgactgc tgggccttga tactgtcctc
     7801 caaggcaatc cgcagggaga tttccccgtc gcttcttctg agcggccccc ggtcaatggc
     7861 aatgcccttg atccgccggg tgatccgttc ctcggtgtaa tcctccccca aggtcttgca
     7921 gcgggtgaag cgttcctgtc cgggagcgcg gaatgaaata aacttgccct gtttgacttc
     7981 atagccctgc gtctccatga gccgcaaaaa gccgtcaaag tctttggcct gcggaatggc
     8041 cgcgtcgatg gcgattttca gttttgcttt ccagctcttg ccctttttct gtgcgtccca
     8101 ctcggcatag gttttgccct tgtccttgcc gggctttacc acagacaggc cgtgttcctt
     8161 gcacagccgg tcgctggtgc gccggataaa ggcatagctc tggcggttgg aaatgtactt
     8221 gcgctggttc gccatatcca cggcgcagaa aatgatgtgg ttatgcagat ggcccttgtc
     8281 aatgtgagtg gtaatcacat aggggtattt gccttgcagc acttcatcgg caagctgccg
     8341 cccgatctcg tgggcctgct ccgccgtggt ttcccccggt tcaaaggctt gtatcaggtg
     8401 gtgggccaga ttgttgccct tttggtacgc ctgatccaac agcatttgaa attcaatatc
     8461 cgccgtttca taggaacagc cataggaaga aacaaacagc ttttcatcgg tcttgtccgg
     8521 gttctcgatg tagtccagcg cctttttcag cgttgactta ataggatgga tctttgtaac
     8581 tgccatatct gtgccagcgc ccccttgatg tcctccaagt cctgcgcgta cacagtcccg
     8641 gtactgttga tacgccttgc aatctggttg atgttgacgc cgatcttttg cagctctgcg
     8701 gtctgcgccc ggacggggcc ggtgtcgata tggacgatat agccgtcaat cagcattttc
     8761 cgggcgtagg cggaaaagtt ctttgtgcca agctgggcca tcttctgctg gatcagcgcc
     8821 cgttcctccg gcgtcaccgg gacgcgcagc acaaacttcc gttttcggtt tgccactttc
     8881 gcacctccat tcattcaggg gtttggggac ttccccaaga tacaaacggg aagccgtttg
     8941 ccgggagtgt ggctacactc cctatgcttg ctgtctttgt tcttcttccc ctaaaaagcg
     9001 ttctgacccc aaagaaatac ccgcaaaatc ccggtttttc cgaaaaccgc acaaaaaaag
     9061 agagggcgca gcctgcgtcc tcccaaaaac aaaacagagg gttgtcatgc accctccgtt
     9121 tcgtcattct tctgttggat ttgaatggcc ccgtcaatcg cgccctcgat gattggcaga
     9181 aattcttccg ggcagagttt cagcttgtgg ctgacccgct gccgctgtgc gctttcctcc
     9241 cgcatgattt ccgggttaaa ataccgttcc acgggaagcc cgcagatttt gataaactgt
     9301 atgataaccg gcaggctggg aatcgtgccg tcattttcga tgttggcaag atagcgccag
     9361 tcgatgttga cctgttccgc cagcgtctta cgcgataact gcttgacttt ccgcgccgct
     9421 tttacatccg cgccgaaggt ttcaaagccg ggacaatctt tgatatttgc catatagcac
     9481 cacccatcta cattgtatat ttcatatttt ctcaatggaa tgttgctata tgcagtaata
     9541 ttaaacttta taattcatat tttgcgcctt gtacggaaaa gcgggctatc ctataataaa
     9601 gaaaaagcac ttttttgaaa aaatccgtcc gctgtaacat ttcgcggaca tttgcctgtt
     9661 atactatctg caaaaagcaa aggaagtgag gatatgaagc agattttaat tgtcgaggac
     9721 gacagttttt tgaataagat gttagcctac aacctgaccg cagacggcta cggcgtaact
     9781 tctaccctaa atgccagaac cgcagccgac gccatccgcc agcgggaatt tgatttagtg
     9841 ctgctggaca tcaacctgcc ggatggcaac ggttttgagc tgtgcaggct gataaaaccc
     9901 cagcacccgg acaccattgt aatcttcctg accgccaacg atcaggagag cgaccagata
     9961 cggggctatg aggtgggcgc ggtggactac atcacaaagc cctttgtgat tggagccttg
    10021 cagcggaaaa tcaaagccat gttcgccatg ctggaacacc acaaaccggc caaggacatt
    10081 tacgacgacg ggcggctgtt tctagacttc tcggagcaga cggcttcctt aaacggcaag
    10141 cccctgaccc tatccccgat ggagtacaaa atgctgaacc tgttccgtaa aaatccccgg
    10201 caagtactga cccgtgggca gcttttggaa aagctgtggg atatagacga gaagtatgtg
    10261 gacgaacaca ccctgacaac ctccatcagc cggattcgga gtaagatcga atccgacggc
    10321 ggcgcaccct acatcaagac cgtttacggc atgggctatc aatggacggg aggcgaggca
    10381 aaatgaagtg taagaacctc tcggtaaagc ggctgtttgg ccgggtggca atagggcttg
    10441 tcctctccat gtccgggatc accatagccc tgttttttgt gacaaaacag attgcggtgc
    10501 tgctgacggg cggggcgttg ctgctgtgcg cccttgtggg gatttttgta ctgacgcagg
    10561 cgtttggaaa gcggctgtcg cagtttaccg ctgacctgtg ccagacctta gaccacatga
    10621 tcgcagggaa tgaagcgccc cagcgcccgg aggacagcga aacccagctt gccagaatcg
    10681 gacaccggct ggcaaggctt taccagatca tgcaggagaa ccgccgcaag gtagacgagg
    10741 aacggcagga gttacagacc cttgtatcgg atatttccca tcaggtaaaa acgccggtaa
    10801 gcaacctgaa aatggcgacg gacaccctgc tggaaaagcc catgaccgag acggagcgca
    10861 ccgattttat ccggggaatc cgcagccaga cggataagct ggactttctc tttcaggccc
    10921 ttgtgaaaac ctcccggctg gaaacgggcg tgatccagtt ggataagaaa acgggccgcc
    10981 tttttgatac tgtggcgcag gctatgagtg ggatcgtgta tgcagcggag aaaaaggaaa
    11041 tcgccgtgtc cgtggactgc ccggaggatt tgaccgtttc ccatgacagc aagtggacat
    11101 ctgaagccct ctttaacttg ctggacaatg cagtcaagta cacctcggca ggcgggaaaa
    11161 ttgctgtgtc ggtggtgctg tgggaaatgt atgtggagat caaagtgatc gacaccggca
    11221 agggcatttc cgaaagcaat caggccgcca tcttccggcg cttctatcgt gaggaagaag
    11281 tacacgaaca gcaaggtgtg ggcattggcc tgtatctggc ccgcgagatc gtaacgcggc
    11341 agggcggcta tatcaaagtg gtttcggagc cgggcaaggg ttcggaattt tctattatgc
    11401 tgcctacaaa atagaacgcg gcggacggcc atttccggct gcccgccgta aatttttttg
    11461 aaatgtccga gcgttgtaac atttcacccc gattttcaat gaaatttttt ggccgctgta
    11521 acatttcgcg gacatttggg ttttataata cccttatcaa caggcaggaa gccttgaaag
    11581 gagttttgaa tatgagcgtt ttacagacta tcgacctgaa aaagtattat ggcacaaagc
    11641 ctaacattac ccacgccctt gacggtgtga acttctccgt ggaggacggc gaatttgtgg
    11701 ctattgtggg aacatctgga agcggcaaat ccaccttact tcacatgatg ggagggctgg
    11761 acacacccac ttccggcaat gtgattgtcc gggacaaaga actgtcgaaa atgaacgatg
    11821 aacagcttac tatcttccgc cgccgtaaca tcggctttat cttccagaac tataaccttg
    11881 ttccgatcct gaatgtgtat gagaacattg tcctgccagt ggagctggac ggggacacgg
    11941 tggataaaaa gtttttgaat gagattgttc atctgctagg gctggaagat aaactgaaaa
    12001 atatgccaaa taatctatct ggcggtcagc aacagcgagt agcgattgct cgtgccttga
    12061 ttaccaaacc ggctatcgtg ttggccgacg aaccgaccgg caaccttgac agcaagacca
    12121 gcgccgaggt gctggggctt atcaagcgca ccagtgcgga gttccggcaa actgttgtga
    12181 tgattaccca caatgacgac atagcccgtc ttgcagatcg gattgtccgc attgaggacg
    12241 gcaagattgt ggagtaagga ggtggcaagt tatgacatgg ccttttgaga acgatactag
    12301 cgctgttgta aaaaagttaa caaaacgcag tatcctagcg gataaaaaaa gcaatttact
    12361 tttgatagtt acaattgcac tatctgtatg tatggttctt tcacttgcac tatcaacaaa
    12421 aggaatagcg gaagcacaga aagattcgta tcgggacaaa gctcagatta cggttgtcgc
    12481 tcctactgaa gaacaattgg ctcaaataaa aaataatagt aatgttcaat ggatcggtga
    12541 atatctgttt ctgggcagtt cctatcagga caatatagaa ttagggattt cttacgcaaa
    12601 tgctgattat atcgaaaaag agcgtggatt ggtttatagt ggtagtttac cgaaagatga
    12661 taatgaaata ctgttggagc aagattacat tgatcactac gcaccaaact gtcaggttgg
    12721 gggtacaatc gttcttgatc taactggaac gggagaaaca actgaataca gaataagtgg
    12781 aattattgca aatagcgaaa atacccttgg tgaagcatat tatgatgtat ttgttagcaa
    12841 gacatatgca caaatgctat tgagtaaaac acttgataga gattttcaaa ttacagcata
    12901 tacaagatta aatacctcag caatttcctc tggagaaata ttgtcatttg cttatgatat
    12961 tacgcgtcca gttggaattg acgatgagca ggtttttctg actgattatt ttgccgttat
    13021 gtctggcgtt atcaaggatg gattgtatat ctctattccc ttgattactt ttattacttc
    13081 tgtattggcg gcggttgtaa tatatggtat tttttatacc gttgttgtga aaaatgttca
    13141 aaccattgga caacttagaa ctattggaat gactaaacgg cagattaaaa aaatggtaag
    13201 acgaaacagc ttgcaatttt ctgcaaaagg tattttctta ggtatttgtt tgggaaccgt
    13261 tattggcttt tgcgtttgtc caagtggctt tagaataaaa actgcattga tgtatggaat
    13321 atttgcaaca attatttctg cttttgcaat tacaattgct gtgcggaaac ctattaaaat
    13381 tgcatattca acttcaccga ttgctggtat gcattatttg ccctatgagg tcaaaaagaa
    13441 aaatagccac aaactgcatc gtaaactgac ttctgcaaat ttggcaaaaa taaacattag
    13501 ccgaaacaga ggcaaagcga tatttacatt tttgacattg agcattagcg gtattatatt
    13561 tctcacttct gctcttgtcg ctggttctat tgatgctgaa aaacaagcac gatttctgta
    13621 ttttcctgat ggtgaaatac aaatccagtt gagaaatgcg gcacaatcca cctttactgc
    13681 aaatggagaa tacaattaca gtacattttt gcaattagga aataacccat tggagaatgt
    13741 tgattttatt gaaaagattg aaaagatgga tggagttgag aaggtgactc ctcacaatgc
    13801 tattatgact acaattggtt tgcctgttgg aatgggcaat tggctatatc gaagcaatta
    13861 tgcaccaaca cttacacaag aacaattttc cattgtatct gaccttttga cggcgggttc
    13921 aattgattat accgaaatga caaaaacaaa cgggattttc gttgaggaca aatatgcgtc
    13981 tgttggagaa acattaactc tgcaattgcg aggcattgat ggtaatgaat tgagcttgga
    14041 tgtcccagtt gttggcacat atgatggatc aaagttaatg agtgtatacc cattggttcc
    14101 tggtaatcca aatttcctta tgacttatga tactgtcaaa aatcttactg gcgtatcagc
    14161 acaaactgga atattgtcta tagatgtaac aaaagagaaa tatgatgctg ttttatcaca
    14221 gattaacgaa atagcccaaa gtggtgatga aatcattgtt tttgatattg cacagaatat
    14281 ttcagggata aaaactgaat ataatgcatc tattaaaaac ctctatctgg tttctgtaat
    14341 tctatttttg tttggaggaa taagccttac caacacactt ttggtggatt tcaaaaacag
    14401 aaaacacgag tttgctttac taaaggctat tggaacaacg caaaagcaac taaacaatat
    14461 gcttaacaga gaaattatga cctatcttgc aggatctttt tcaattgctc ttttgggaag
    14521 cactattgca agcataattg tatgcaaaag attagattcg ataaatcact gcattagatt
    14581 ttcattccct tggctgttcc tatttgcttt tgttgcattg cttataatca tttatttcat
    14641 attctcgttt tatgtttcaa gagagttaaa aaacacagat gttataaagc tgatacggga
    14701 ataggtatca tcggaggtat aacttatgga aaaagaaacg atgggaactg taatttcagt
    14761 tacaaaacaa tggtggttaa aagtgaatcg taaacctgcc cgcgctcatg ctatggatgg
    14821 cgcagccttt ccgcatacta tcaaggtcaa atatacgata gacggcaaag attacatctg
    14881 ccgaaaatgg attggtgcag gaaacaaaat tcctgacaag ggaacaacga ttaaagttac
    14941 ctattatgaa gataagccat cgaaagcaag aattgagctg taatcatcgg aagaattgat
    15001 gtatagaagt agcaaaattt gtgatttgga ttttctattg tcaaaatatt attgatactt
    15061 aataccgtgg aaaggagatt ttttatgagt aaatctgaaa ataatcacga taatagtgat
    15121 tttcaaatgt tgaaggagga aatttccgaa agcgaatatc aaaatgccca atatgaagct
    15181 gaaaagaaaa agcatatgtc ttattgggta atgattattg cgccattcat tatgatactt
    15241 gtaatattta tattttatta ttgcttttat cggtagaatt tatttttcac attatggtag
    15301 aactgaatca atctaaacat ctgcctccac agcggggaaa agaaaaaaac cgctgacggg
    15361 cagaggattt cgtgcgcttt cctgcatcac tgacggatac ggcggttttg agtagatcaa
    15421 ggccgccgtt tctgtttgct tcgacgaccc ctgcaccgtg ttctggcatg gcggctttag
    15481 gagggagccg ccatgcaatg taaaccatat cgacagaatc cgacagggat aagcctgtca
    15541 aaaaaagtct gtccccgcca tcggggacaa gcggctatga gtatgtacta taccatgtag
    15601 gaaaagatca tattgctttt tgtttcgctc tgtgggtctt tgacctgccg ggcgaatttt
    15661 gttatttcct gcggtctgct atccggcgca aaaagaaatt tcaaaaaagt gaaaaaagga
    15721 ggcgtttagc gggcagccgg agagattcca ttcgctctat tggcggaaag ggagagaacc
    15781 accacatccc ccatagaaag ggggtgagaa gatggaagcg atcccccgca aatacggtga
    15841 atggggccag tttgaccgct actgcaagct ggttctgtac catgaggcga tagattatct
    15901 gcgggaaatg aagtgccgcc gcgaccgtga agtgtcgctg gacgctgttt ccccggctga
    15961 ttgggacaag ctctcgaccg tagatcggta ttcctgcgac agctttacat tcagttcgca
    16021 tggctatgac ctacatatcg acaacgagct tgtggccgag gcgtttgcca gcctgccaaa
    16081 agacgagcag agtattttga ttctccgctt tgttctggat ttgactgacg aagaaatcgg
    16141 ccgcctgatg ggaatgtccc gcagcgcagt acagcgccac aggacaagca ccctgaaaga
    16201 cctgcgtata aaattgatgg cgttcatgcc ggagggaggt taaacgcgga tgaaacaatc
    16261 cagttacaag ggaaaaagcc ctctgcctga ttttgtgatc gacgcggcct gcgccggtaa
    16321 cgccgaagcc gtggagcgtg tcttgcagca ttatgacggc tatatcaata agctgtgtac
    16381 ccggacgctc tatgacggca gcggccagcc tcatatctgc attgacgagt atatgaagcg
    16441 ccgattgcaa atcaagctga tccattccat cgttagtccc attggcgact gacggttaag
    16501 gccccggcca tagacaggcg gctttccctt tccccgcttg cctgttttcc cggcggcccc
    16561 tttgctcctt gacaaatacg cccgcaggac aatggcggcg cgtcataggg cagacggata
    16621 cattctgtct gcgccgagcc gagcggcagg tgcgccaaga ggctttccct cattgggata
    16681 agcggagcga ttttaccggg ccgtaaagca ggtgtggcag cctgcgggcg acgacgcgca
    16741 gacaggacaa tgatactccc ttgtcgtcgt agtccgagcg tttgaagcgt cgcaggcaac
    16801 gagcagggcc gcgtgagaac cacgcggggg tgagattccc gtggagctgt gccagcagcc
    16861 atccgttatt ttctgcccac tttcacaaca ataacctttg ttttgaaaag aaagggggcc
    16921 atgttatgag taataacgat gtgcctattt gggaaaaata cacgctcacc attgaggaag
    16981 cgtctaaata ttttcggatc ggggaaaaca aactgcgtcg gcttgccgag gaaaacccca
    17041 ccgctggctg ggcgatcctg aacggcaacc gcatccagat caagcggaag cagtttgaaa
    17101 aaattattga ttcccttgac acaatatagt gaaaaagggc cttgcgcgtg ttataatagg
    17161 ttataagcgt gtcaaggctc tttccgtcat ggaaaggagc atgacgaaat gccaagaaaa
    17221 gaacgagatg aaaaaagacg ggacaacaaa ggccgccttt tgaagtctgg agagagccag
    17281 cgatcagatg gaagatacgc ttacaaatac attgacacct ttggagaacc ccagtttgtt
    17341 tactcatgga agttagtacc taccgataaa gtacctgccg ggaagcggcc ctgtctttcc
    17401 ctgcgcgaga agatcaagca gatacagaaa gaccttgacg acgggataga caccatcggc
    17461 aagaaaatga ccgtgtgcca gctttatgaa aagtacaccc ggcagcgcgg gaatgtgcgg
    17521 aaaggaaccc aaaagagccg ccagcagttg atgaaacttc tgtccgaaga taagctgggc
    17581 gcggccagca ttgacagcgt aaagctgtcc gacgcgaaag aatgggcctt gcgtatgcag
    17641 gaaaagggcg ttgcctacaa caccatctgc aacagcaagc gttccttaaa agccatgttt
    17701 tacatggccg tacaggacga ctgcctccgc aagaatccct ttgacttcca gatcaatgag
    17761 gttatcaacg atgataccgt accaaaggtg ccgcttaccc ctacacagga aaacgagctt
    17821 ttggacttca tgcagaacga tcccgtctat gccgagtatt atgacgaggt ggtgatcctg
    17881 ctggaaaccg ggcttcgtat ttccgaactc tgcggcctga cgcccgccga cctgaatttt
    17941 gaaaagcgct ttgtgaatgt agaccaccag cttttgagaa gcaccgagga cggctactat
    18001 atcgaagcgc caaaaacaga aagcggattc cgtcaagtgc cgatgagcgc agccgcctat
    18061 gaagcgtttg agcgcgtgtt gaagaagcgc agggacggca ggtgcattga ggttgatggt
    18121 tataaggact tcctgttcct gaacagggac ggcctgccca aaaccgccgt caactatgac
    18181 gccatgttca agtgccttgc caagaagtat aacaaatgcc acaaggagcc actgcccgat
    18241 gtgatgacgc cgcacaccat gcggcataca ttctgtacca gaatggcaaa cgcgggcatg
    18301 aaccccaagg cattgcagta catcatggga cacgccaaca ttgtaatgac gctgaactat
    18361 tacgcccatg ctactttcca ttccgcacag gaggaaatgg aacggctgca agccaaggca
    18421 aaaaccaccg cagaggccaa gccggagctt gcggcggaaa gcaccgagga aagcaaggcg
    18481 gcataagtag tacgcagaat ttactactgc ttttactact tttgagagtg aaaacataag
    18541 gggtaataaa aagatttgtg aggttcttcc gatatgaaaa gtgccggaaa agcccggaat
    18601 aacgggctat accggcatat aagaacatct aaagagataa ttagaaatct attaaatgat
    18661 ttagctaaag ataacataaa aaaatacaaa aaaggtaata tattccatat tgatatacaa
    18721 atatcttcta atttagaaga tataaaaaaa gcctttaaaa ttaaaacaat tggcactgct
    18781 cctaatttta aataacaaaa aaattactag ataatctaaa aacaggatgg cattatgaga
    18841 acaataacta aacttcctat atcacatatt ttgtaattag tgaaaagcaa attgactact
    18901 atcataagtt aatgactctc atcttaatat ttaaaatata ttcatatttt tctattttat
    18961 ctaaatgcga ttttcatatc taataataga aaaataaaca attaacaata aaaaagtctt
    19021 catttattgt taagggagcg tca
//
LOCUS       DACEOM010000062        18886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEOM010000062 DACEOM010000000
VERSION     DACEOM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.063"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(78..150)
                     /locus_tag="IW781_002127"
     tRNA            complement(78..150)
                     /locus_tag="IW781_002127"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="IW781_002128"
     CDS             531..1100
                     /locus_tag="IW781_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1183120.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="IW781_002129"
     CDS             1087..1947
                     /locus_tag="IW781_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1183121.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /locus_tag="IW781_002130"
     CDS             2137..2841
                     /locus_tag="IW781_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1183122.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="IW781_002131"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="IW781_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1183123.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /locus_tag="IW781_002132"
     CDS             4678..5994
                     /locus_tag="IW781_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183124.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="IW781_002133"
     CDS             5995..6864
                     /locus_tag="IW781_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183125.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="IW781_002134"
     CDS             6919..7728
                     /locus_tag="IW781_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1183126.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="IW781_002135"
     CDS             complement(7831..8460)
                     /locus_tag="IW781_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1183127.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="IW781_002136"
     CDS             8630..9922
                     /locus_tag="IW781_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1183128.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="IW781_002137"
     CDS             complement(9998..10660)
                     /locus_tag="IW781_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1183129.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /locus_tag="IW781_002138"
     CDS             10885..11169
                     /locus_tag="IW781_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1183130.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="IW781_002139"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="IW781_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1183131.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(12934..14082)
                     /locus_tag="IW781_002140"
     CDS             complement(12934..14082)
                     /locus_tag="IW781_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1183132.1"
                     /translation="MLKVWVEQTKDGRFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14181..14486)
                     /locus_tag="IW781_002141"
     CDS             complement(14181..14486)
                     /locus_tag="IW781_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183133.1"
                     /translation="MEKNLGQHIITCKNCGSNRVSVTSPRSNGCMLFFIGLMLLSFGF
                     WIPIIGWLIMIPLGGIMMLLSFFVPLLQKNYSVTCKACDNKFEVSKKQYQKYIKEIK"
     gene            complement(14573..14995)
                     /locus_tag="IW781_002142"
     CDS             complement(14573..14995)
                     /locus_tag="IW781_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1183134.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADKIAIMILLNIFIENELTDE
                     TQFKLENFMKFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(15013..15333)
                     /locus_tag="IW781_002143"
     CDS             complement(15013..15333)
                     /locus_tag="IW781_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183135.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKEAPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            complement(15781..15969)
                     /locus_tag="IW781_002144"
     CDS             complement(15781..15969)
                     /locus_tag="IW781_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183136.1"
                     /translation="MSRKTSVKISGDLEKIIKSEANKILKKGVDYTCPSCGKKIKISN
                     GKNKCKYCGAEIDFKGKV"
     gene            complement(16156..16407)
                     /locus_tag="IW781_002145"
     CDS             complement(16156..16407)
                     /locus_tag="IW781_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183137.1"
                     /translation="MKDYKLDFQSELPIEFVNEFKIEAPDKTRTLFTFNHDMILQEFE
                     DDTVICVARDSEFLTIYSNRKFNQTIPVRSKKVIIYFEE"
     gene            16483..17229
                     /locus_tag="IW781_002146"
     CDS             16483..17229
                     /locus_tag="IW781_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1183138.1"
                     /translation="MNTPQIFNFEQNEVRTFLENDIPYFVANDVAKTLGYKNPSDATN
                     KHCKKAVKTWGSDSLGRRQSFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            17244..17447
                     /locus_tag="IW781_002147"
     CDS             17244..17447
                     /locus_tag="IW781_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1183139.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            17462..17761
                     /locus_tag="IW781_002148"
     CDS             17462..17761
                     /locus_tag="IW781_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183140.1"
                     /translation="MKKPTLSELIEATEKAANPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFSEGIVRPGHSTTFIHYHTFIWFLKWKDANKYRVKILSPSDVLKEAS"
     gene            17809..18093
                     /locus_tag="IW781_002149"
     CDS             17809..18093
                     /locus_tag="IW781_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183141.1"
                     /translation="MKITVPDELIADELTEQIVRKVLDALDERLKVMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWINLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            18114..18302
                     /locus_tag="IW781_002150"
     CDS             18114..18302
                     /locus_tag="IW781_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183142.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            18624..>18886
                     /locus_tag="IW781_002151"
     CDS             18624..>18886
                     /locus_tag="IW781_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183143.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRCNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYD"
BASE COUNT         6311 a         3233 c         3844 g         5498 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtagtaatt aagaaagaac cctattttaa cagggttctt tcttcttttt ttgttttatt
    12901 ttaagcaaaa ggggcaaaga aggggcaaaa atatcaaagg ctatttagtt tatcaattac
    12961 ttgttgtttg gctctttttg ttacatggtt ataaatagac agagttgtgt ttgcatcaga
    13021 atgccctaca cgctccataa tggctttaag aggtacgcct aattcagata atagtgaaac
    13081 gtggctatgt ctgaatatat gtgaggatag acttttttct aattccaact cttcctctac
    13141 tttatggagt atagcgttaa atgagtgcag cgcaagcgga gtgccacttg tagatataaa
    13201 tatatattga tcgggatctg tgggtctacc tgcaagaata ttgtcagcta tcacactttc
    13261 aatcaattct tttgcacgat tgggtaattg cacttcacgt tgcgaataag tattttttgg
    13321 agttgttttt atagcattat ccattttcac agatgtgtaa tctaaggtcc cattaatgga
    13381 aattttccca tcctcatagt ccttcatttg caaagctagc aattccccat atctcaaacc
    13441 agttaaatat agaaactcag ctattatgcc gtgtagtttt ctgcgaggat tggagtatag
    13501 ctgtttcagt atttgatcaa tttcttcttt ctccagatat tttttattca tagaaagcct
    13561 tcttttttct tcttccactt ttttaggatg gattttaact gctagcgcag ggtttctttg
    13621 aatgtatttt ctatcgattg catagtttag cataacagat agagttgttt ttgtttgttt
    13681 tgtgtagttc agtgagaggt caccaaacgt atacatatct tcaattatct tattaatgag
    13741 tgtctcatca atgtttctaa cgatcgtatc atcgcttatg tgcttagaaa catgtttcat
    13801 catcattgga accttcaaat agctagtacg tttaacatgc tgcttataat attcatacca
    13861 ttctttatac aactcaccaa aagtgatatc tgatttattg taatcttcga gtgcttcttt
    13921 aattttttta tctagaattt tctgagcttt tttccacgct tgtggtgaat tacttgtaag
    13981 tgttgtagat tttttccgtg ttttttctgt ataaggatct acatatcttt caataaactt
    14041 gaatcgccca tctttggttt gttcaaccca cacttttaac atctcctatc atttgctata
    14101 ataggcataa caaatagacc tatataggtt tgttttctaa aagcacgctc ttactctgga
    14161 cggtggggcg tgttttatta ttacttaatt tctttaatgt atttttggta ttgtttttta
    14221 gaaacttcaa acttattatc acaggcttta cacgtcacgc tataattttt ttgtaataaa
    14281 ggaacaaaaa aagataatag catcattatt cctcctaaag gaatcattat taaccatcca
    14341 attataggaa tccaaaagcc gaaacttaga agcattagtc ctataaaaaa aagcatacaa
    14401 ccatttgatc taggggacgt tacagaaact ctgttactgc cacaattttt acatgtaata
    14461 atatgttgac ctaaattttt ttccatccta actctccatt tctatggtaa aatagatttg
    14521 actttttaaa tgaagtcgct ctcataagtc cgtgttgcag cacgggcttt ttttactgtg
    14581 cataagagta ttttttcttg aaatatgact ggcaaacatt aaaacattct gttcttaact
    14641 tattattgat agagtagaac ttcatgaaat tttctaattt gaactgagtt tcatcagtca
    14701 gttcgttctc aataaagata tttagtaaaa tcataattgc aattttatca gcttcagttt
    14761 cgaattttga atgaaaagtt gtagagttat cgtacagtac tgaatattca aaatgtgaag
    14821 caatgaaatg accgagctcg tgggctaaat gaaaagcttc agaactgtct tcgtgtagtt
    14881 tttcattcaa aaatactatt cttggttttg gataataaaa acctggttct tccatttcca
    14941 tatagatcaa ttttaaatta tactcactca gcatttcttt caactttaaa tacatacaaa
    15001 ccatcactcc aactattcat tttcctctaa agctttagcg attgcaatcg ctttacgcat
    15061 tgtctcctta gatatttctt ttccgtcaaa agaaaaaaca gtatcgtctt ctgataaatc
    15121 cacatgttta ggggcctctt tttcttcgcg ccccagaaga tagtctacag agacatcgaa
    15181 atagtcagca atttcttgca aacgttctgt agaggggttt gagttcttaa gtctatataa
    15241 gacgttttta ccatagccta ggtcttcttc aactttattt aatgattttc cctgtttttt
    15301 cgctaattct tttattctct caaacgttat catatcaata ttcctttcgt tcaagaaaaa
    15361 atatttaact ttatttgtta aaatatgttg actattttaa caaaagatgt taatatcatt
    15421 cttgtaaaca agtttaacaa ctaaaaagac aacaaaaaca cttttgatta ttaaacgcca
    15481 accgccaaga aagcttttaa aatcaatgtt tatatgtctt atttaactat gttctgattt
    15541 taacactgta tgttaattta gtcaacgcag ttttttaaaa agttgttaaa tttgtttacg
    15601 aatataaaag aaaggagaga aatagatgga aaataaaaaa actggattaa taactgtaga
    15661 aataatcgga attgatgatg caacaaaaaa agctgaaaga tatattgagt tattaaaaga
    15721 agccaaaacg ttggcagacg aattggcttc aaaggaattt gaaattgaga ttaagcagga
    15781 ctaaaccttc cctttgaaat caatttcagc tccgcaatat ttacacttgt tttttccatt
    15841 agatatttta atttttttac cacaggaagg acaagtataa tcgacgccct tttttaaaat
    15901 cttgtttgct tcggatttta tgattttttc aaggtcgcca ctgattttaa cactggtttt
    15961 tctactcata gatccacctc actttctaca gtgagtatac cagagaagga ggtaacaaca
    16021 tgaaaattag cattgaagca aagccacaag aaatagcaga attgctccaa gctatcggaa
    16081 gtagcaagga gcacactaaa cgcaaacatc cagaatcgaa aatagaatat gatccgcaga
    16141 cgggtgaagg aaagcttatt cctcaaaata aataataacc ttcttgcttc gtaccggtat
    16201 tgtctgattg aatttacggt tcgaatagat agttaaaaac tcactatcac gagcaacgca
    16261 tattacagta tcatcttcga attcttgaag aatcatatcg tggttgaaag taaataatgt
    16321 acgagtttta tctggcgcct caattttgaa ttcatttacg aactctatgg gcaattcaga
    16381 ctggaaatcc aatttatagt ctttcaaagt tgccacctcc ttatcaatta tttcagcctg
    16441 tcacactgat aaggaaatta taccaaagaa aggaatgaaa aaatgaacac accacaaatt
    16501 ttcaatttcg aacaaaacga agttcggacg tttctagaaa atgacattcc gtatttcgta
    16561 gcaaatgatg tcgctaaaac attaggatac aaaaacccga gcgatgctac taataaacat
    16621 tgtaaaaaag ccgtaaaaac atggggtagc gattcgctag gtcgtcgcca atctttcaaa
    16681 gttattccag aatcagatgt ttaccgcttg attatcaaat cgaacttacc aagcgctgaa
    16741 aaatttgagg cttgggtaat ggaagaagtc cttccaacaa tcagaaaaac aggtagctat
    16801 tcaaatgtac ctcaaagttt tgcacaagca ttgcgtttag cagcagattt agaagaaaag
    16861 aaccaattac tcgaacaaca aattgccgag tacgaaccaa agattagcta cttagatacg
    16921 attctttcat cgacagatac ggtagcgact tctcaaattg cagctgatta cggaatgtcg
    16981 gcaattgctc taaacaaatt gcttaacgag ttaggtgttc aacataaagt tagcggacaa
    17041 tggatacttt accgaaaaca tatgaaccaa ggatacacaa aatcgcacac aagtgagata
    17101 ccgaaagccg atggcggcac taaagttgta atgaatacca aatggacaca gaaagggcga
    17161 gtgtttattt acaacttatt aatcgcagag ggctattacc ctcaaatgga tttagaggaa
    17221 attggttaga aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag
    17281 aaaaaatcaa agaatatgtc gaaagttcga agttcttaac ttacaccatg cttgctgaaa
    17341 gatttggaat tagtaaaagc tatttatctt taattttaaa cggtaaaaag acttctgcag
    17401 aagcaaacag aattatagat tcgattatca ctatgtacga attgtagagg aggaaaacga
    17461 aatgaaaaaa ccaacgcttt cggagttgat agaagctact gagaaggcag caaacccaga
    17521 cgattggtat cgtcaaagtt tgatcttgga gaagttccac ggcatgtcaa aaactacttt
    17581 agttgaatac tgcaaggaaa tggaaacaat tcctgaattt tcagaaggaa ttgttcgtcc
    17641 aggacattca accacattta ttcattacca tacttttatt tggtttttaa aatggaaaga
    17701 cgcaaataaa tatcgtgtaa aaatattgtc tccttcagat gttttgaagg aagcaagttg
    17761 attattttca gagtaaaaag taaacaaaaa tattaggagg aaaatttgat gaagattaca
    17821 gtaccagatg aattgatagc agatgagttg acagaacaaa tagtaagaaa ggttttagat
    17881 gcacttgatg aacgactgaa ggtaatgaac aagtcagtgg agcttcctcc atatccaaac
    17941 aaatcagagg taaaaaaagt tttaggcatt ggtgatgaca aattaacaca ttggataaac
    18001 ttaggcttaa aaacacagca gtggagcaag ttagacatca gaattgaacg atcagaactc
    18061 caaagatttt tgaaagaaaa ctttgagttc taaaggcaaa ggagaatgat tttatgtcct
    18121 acacattgca acaagaacat caaattctcg gtttgattaa acaacgcagg aaacaattac
    18181 aagatgaccg tgcagcgctt agaaaagccg atgagctatc agatagacaa gctgaactaa
    18241 ttgcttctga acttgaggat ttgagaatgc tagaaataaa aaatagggag attagattat
    18301 gaagaagaca gacacacttt ttataggatt cattttgggg ttattagtga ttgtagcgca
    18361 ccaaagtatt attgggggaa gcttgttcgc agcattgatg gttttaatca atctgcttga
    18421 ttcaaaagaa aggagcaact atggcacgag aagaagcgct aaaaatcggt aaagtgattg
    18481 ctgataattg gtgggcaaat agccgtccta ttattttaag caagcaacat atcgacaagc
    18541 aaaaagcttg gcaacaaata aaaagcgact ccgccggcaa gcattgagtc gcaaacaaaa
    18601 tacatctaag gagatgttac cacatggaaa aagaactttc cactctagat caatatttga
    18661 ttgatcctga ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagatgta
    18721 atcttctgac agatgaagaa ctagcatgtg atcaagatga tttgggcaat tttgtgagca
    18781 tttgggatca tgtttacctt attcatctat caaaacattc aaacaaacct gaatacatct
    18841 atgtcatcga agatggcttg attgatgcgc tagaagagta tgacag
//
LOCUS       DACEOM010000063        17707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEOM010000063 DACEOM010000000
VERSION     DACEOM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.064"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(37..495)
                     /locus_tag="IW781_002152"
     CDS             complement(37..495)
                     /locus_tag="IW781_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1183144.1"
                     /translation="MKILVSACIMGVDCKYNGKNNKNLAAINYLKDKEIICICPEQLA
                     GMTTPRPCAEIVNGVVTDENGNDVDLEYKRAVSMALSQIQHEDIELAILQSRSPTCGV
                     NQIYDGSFTGKLISGMGLFAKALKERGIKVIDVEEVESFNLLDCPQLQSI"
     gene            complement(609..1889)
                     /gene="ltrA"
                     /locus_tag="IW781_002153"
     CDS             complement(609..1889)
                     /gene="ltrA"
                     /locus_tag="IW781_002153"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1183145.1"
                     /translation="MEQEPKGIREQSAKYPAVQNLMHNVNERTLMAEHRKQSRKKATG
                     VDGIDKAAYDENAPERIAALVERMRKFQYKPLPVRRTYIPKANGKLRPLGIPAYEDRL
                     VQGVMAKVLNEVYEPRFLGCSYGFRPGKGAHDVVRYINQTIMTRKVNYVLEADIKGFF
                     DNVDHDWLMRFLVHDIQDKNYLRYVKRFLIAGIMEDGKRLASDRGTPQGGLISPVLAN
                     VYLHYVLDLWMEKAIKPHLRGEVYYVRYADDFLILFQYENEARKVMDTLKKRLGKFSL
                     EVAEDKTRILPIGRFKGTKEDFDFLGFTFFNAKTRSGKYRLGVRTSKKKLKAKRQAAK
                     IWLREQMHRPVAETMRTIASILRGHYNYYGVNGNFHAIQSFWKYLKYSTYRVLNRRHQ
                     KRSMKYNRFLRIWNYYVKEPHLTTDIWGWQPKIA"
     gene            complement(2569..4659)
                     /gene="topB"
                     /locus_tag="IW781_002154"
     CDS             complement(2569..4659)
                     /gene="topB"
                     /locus_tag="IW781_002154"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1183146.1"
                     /translation="MSYQLVIAEKPSVARSIAGVIGADKKQDGYMEGNGYLVSWCIGH
                     LVSLADAGTYDERFKKWRYDDLPILPQEWQYIIPDDKKKQFDTLRSLMERPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCKKPFQRLWISSMEDAAIQDGFAHLKPGTDYDNLYQSA
                     LCRAQADWLVGINATRLFSILYHKTLTVGRVQTPTLKMLVDREAKISSFQKEKYHIVQ
                     IAAGGMEAASERMGNADDAKALKAACETAQAVCVSVTREKKTEQPPRLYDLTTLQREA
                     NRLFGFTAKQTLDYAQQLYEKKLLTYPRTDSQYLTDEMLPTAESLVSGLWPLLPFAAG
                     LDLSPQFGRVLNSKKVSDHHAIVPTMEFVQKGFDGLTEGEKKLLSLVCCKLLCAVAAP
                     HVYEAVTATFTCAGNTFTAKGKTILTPGWKELDRRFKASFKTDADDTAPEPARELPEI
                     TEGQTFDKVTAVVTEHFTAPPKSYTEDTLLSAMERAGADDLPEDAERQGLGTPATRAS
                     ILEKLVQMGFVERRGKQLLPTKDGHNLACVLPEVLTSPQITAQWETELTAIAKGQADP
                     EGFMAGIAEMTRGLIANYSQISEDAQKLFQTERVVIGKCPRCGESVYEGKKNYYCGNR
                     SCQFVMWKNDRFFEERGKAFTPKIAAALLKDGKAKVKGLRSMKTGKTYDGTVLLADTG
                     GKYVNYRVEQRDKN"
     gene            complement(4656..5417)
                     /locus_tag="IW781_002155"
     CDS             complement(4656..5417)
                     /locus_tag="IW781_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAQ1183147.1"
                     /translation="MKKSKLFRTLTLAVAAALCMATTTVTAYAGGADPDPVPLPETTE
                     APAEVPEETEEPTTGGMEPEGVPVTLQGNAALVDDFFGDKQLITVTTKAGNYFYILID
                     RANEDKETAVHFLNQVDDADLQALLEDGKKEPEVCNCTAKCQAGAVNTACPVCKNNLT
                     ACNGPEPKPQPEEEKLQEEKPSGMGGLVVFFVVVLLGGGAALYFLKFKKEKADTTGND
                     DLAEYDFGEDEDDEDETPEPNEDMTSEDGNTEDEV"
     gene            complement(5404..5661)
                     /locus_tag="IW781_002156"
     CDS             complement(5404..5661)
                     /locus_tag="IW781_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAQ1183148.1"
                     /translation="MAMNKLERIEKDIEKTKGKIAELQKQLRELEAAKTEQENLQIVQ
                     LVRGLNMTPQEFAAFVRGGALQAAPTPIPDFEQEDSAHEEI"
     gene            complement(5685..7640)
                     /locus_tag="IW781_002157"
     CDS             complement(5685..7640)
                     /locus_tag="IW781_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ1183149.1"
                     /translation="MEPLKPRDKVTQHMTRDGLTLDNQTTGESVNVSSREAEQEYTAQ
                     PEGAAEKILERADELHDRHKAKKAAKDAGETVVRATGPVSRLQFTAEERASPELAPYI
                     KKAEKRADQLEAAKEALPKKRVVTKETVYDEAKGKAKSRLYFDKVEKAPPQLKPNPAS
                     RPIQEAGLYLHGKIHEVEHENVGVEGGHKGEELAERQAGRMIRSGVHRHKLKPYRAAA
                     KAERKSIAANAEFAYQKSLRDNPELAQAAKNPVSRFWQKQHIKREYAKAARAAGQTAQ
                     GAAGTAKTTAAAAKKAAEKSRQAASFAARHWKGALIVVGVGLMLLLVMGGLQSCTAMF
                     GSTGTGLAATSYLSEDSDMLGAEAAYAALEADLQHELDNYESLHPGYDEYRFDLDEIK
                     HDPYVLTSILSALHNGVFTLGEVQGDLAMLFEKQYILTQTIETETRYRTETRTDSEGN
                     TYTVEVPYTYYICNVKLENFDLSHLPIYILTEEQMGFYAAYMQTLGNRPDLFPNGSYP
                     HASTPKEPTYYEIPPEALKDEAFAAMIAEAEKYVGYPYVWGGSSPSTSFDCSGFISWV
                     INHSGWNVGRQTAQGLYNICTPVSPEQAKPGDLVFFVGTYDTAGMSHVGLYVGNSVML
                     HCGDPISYTNLNSSYWQQHFYCYGRLP"
     gene            complement(7628..8419)
                     /gene="dcm"
                     /locus_tag="IW781_002158"
     CDS             complement(7628..8419)
                     /gene="dcm"
                     /locus_tag="IW781_002158"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1183150.1"
                     /translation="MSALTHFSLFSGIGGIDLAAEAAGFTTVCQCEWADFPMEVLKKH
                     WPQVPKFRDITTVTKEAFFEKTGRETTTLISGGFPCQPFSSAGKQRGFEDERYLWPEM
                     LRVIRELHPSWVLGENVAGFIHLGLDKTVFDLEQAGYAVRVFVLPAVAVGAWHERKRT
                     FIIGHAASHAPCQRHGGCETEPRLGGMADGIPPEMDGHSMWAKEPAQIPYLISDPPAN
                     VAKRLKTLGNAVVPPQVYPILRYIAEIETGRCRNRQQGGDRIWNP"
     gene            complement(8416..10821)
                     /locus_tag="IW781_002159"
     CDS             complement(8416..10821)
                     /locus_tag="IW781_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ1183151.1"
                     /translation="MSKKQRNETSAKAPVKLTRAEKKEIQAVIRKYKGDGKPHSAQAS
                     IPYEAMCQDGVCRVTPHTFSKCIEFTDISYQLAQADTKTAIFENLCDLYNYLDASIHV
                     QFSFINRKIDPKQYAKSFEIRAQGDDFDDIRSEYSGILQEQLVNGNNGLMKRKFMTYT
                     IEADNLKMARARLRRIETDLLGYFKSMGDSAWGLDAKQRLEVMHSIFHPDGEPFSFDW
                     KWLAPSGLSTKDFIAPSSFRFGNARMFGLGGKYGAVSFLQILSPELSDEMLADFLNTE
                     NGIVVNLHVQAIDQSDAIKTVKRKITDLDAMKIQEQKRAVRSGYDMDILPSDLATYGQ
                     DAKELLKTLQSRNERMFQLTFLVLNTADTRQKLENDVFWAAGIAQKYNCSLVRLDYQQ
                     EQGLMSSLPLGASHIQIERSLTTSSVAVFVPFVTQELFQGGDAMYYGINAKTGNMIML
                     DRKRARCPNGLKLGTPGSGKSMSCKSEILSVFLCTPDDVYICDPEAEYYPLVKRLHGQ
                     VVKLSPTSKNYVNPLDINLNYSEDENPLALKSDFVLSFCELVMGGKNGLEAIEKTVID
                     RAVQVIYRPYLADPKPENMPILADLHKALLDQHIPEADRVAQALDLYVSGSLNVFNHR
                     TNIDIQNRIVAFDIKELGKQLKKIGMLIVQDQIWGRVTQNRSKGKATWYFCDEFHLLL
                     REEQTAAFSCEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFICLLNQASGD
                     RKILAERLNISPQQLRYVDNSEPGEGLLIYENVILPFKNPIPKNTQLYQIMTTRLGEG
                     ATV"
     gene            complement(10724..11173)
                     /locus_tag="IW781_002160"
     CDS             complement(10724..11173)
                     /locus_tag="IW781_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ1183152.1"
                     /translation="MAYVTVPKDLTKVKSKVVFGLTKRQLICFGGALLVGVPLFFLIR
                     GRVPTSAAALIMVFSMLPGFLLALYEKHGQPLEVVVRQIVECCFIQPKERPYQTNNAY
                     TALMRQSQMEKEVNAIVQKAKERNERKSAGQAHPRRKERDSGRHPKV"
     gene            complement(11180..11716)
                     /locus_tag="IW781_002161"
     CDS             complement(11180..11716)
                     /locus_tag="IW781_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183153.1"
                     /translation="MLYADPPWRYEQKAVQGAAEKHYPTMSIDELCALPVETLAEKDC
                     LLFLWATFPQLPEALRLIKAWGFTFKTVAFVWLKLNRKSPTWFYGLGYWTRGNAEICL
                     LAKRGHPKRYSKSVHQFIISPVEEHSKKPDITREKIIALAGDLPRAELFARQKIPGWD
                     VWGNEVDSDFSLSVPETR"
     gene            complement(11768..12631)
                     /locus_tag="IW781_002162"
     CDS             complement(11768..12631)
                     /locus_tag="IW781_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183154.1"
                     /translation="MIGDLIGEWIKGILIDGIMGNLSGLFNTVNTKVGEIASDVGSTP
                     QDWNGGIFSMLQNLSETVIVPIAAAILALVMCYELIDMIVEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQQVVNQSAGVIIGDTSIDFDALLPDLESRLEAMDI
                     GPLLGLWFQTLVVGLTMNILSICIFLVTYGRMIEIYAVTALGPIPLATLGNAEWRGMG
                     QNYLKSLLALGFQAFLIMVVVGIYAVLIQQIGTADDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFTAH"
     gene            complement(12703..12918)
                     /locus_tag="IW781_002163"
     CDS             complement(12703..12918)
                     /locus_tag="IW781_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAQ1183155.1"
                     /translation="MEFFNSAVDTLQTIVVGLGGALCVWGGVNLLEGYGADNPASKSQ
                     GIKQLVAGGGVALIGMTLVPLLSGLLG"
     gene            complement(13014..13244)
                     /locus_tag="IW781_002164"
     CDS             complement(13014..13244)
                     /locus_tag="IW781_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1183156.1"
                     /translation="MGHSDEWTFADYFKQEREIYRAIISAAVALRWLIEHDFELPDDA
                     GLKQMEAEVNRELCEAWGEIFSLAVLKWRDGQ"
     gene            complement(13274..15079)
                     /locus_tag="IW781_002165"
     CDS             complement(13274..15079)
                     /locus_tag="IW781_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1183157.1"
                     /translation="MKPEMKKLIIANLPYLLFVYLFGKLGQTYRLAAGADLSEKLLHL
                     ADGFSLAFESAVPSFHLFDLAVGMAGAVALRLMVYCKSKNAKKYRRGVEYGSARWGGP
                     KDIAPYIDPVFDNNILLTQTERLTMNNRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCVSKDYPTSFVITDPKGSLIGEVGQLLVRCGYRVKVLNTINFSKSMRYNPFRYIH
                     SEKDILKLVNTLICNTKGEGEKSAEDFWIKSERLLYSALIGYIWYEAPDDEMNFTTLL
                     EMINASEAREDDPEFQSPVDQMFERLEEKDPEHFAVRQYRKFLLSAGKTRSSILISCG
                     ARLAPFDIREVRELMEDDELELDTIGDRKTALFLIMSDTDTTFNFILAMVQSQLINLL
                     CDRADDKYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLKA
                     IYKDAAEIISDNCDCTLFLSGRGKNAKEIAEVLGKETIDSYNQSENRGAQTSHGLNYQ
                     KLGKELMSQDEIATMDGGKCILQVRGVRPFFSEKYDITRHPRYQYLSDADKKNTFDVD
                     SYLSSLRRKKRRVVTEDEPFDLYDIELSEEDFAAE"
     gene            complement(15076..15552)
                     /locus_tag="IW781_002166"
     CDS             complement(15076..15552)
                     /locus_tag="IW781_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ1183158.1"
                     /translation="MQEEVDQKTIALAVKTGKLTAQVLQAAIRKYLAARQKGTGKAHH
                     GQQSLRQLKKDGSALSNIEITDANIGLFKPCAKKYDIDFTLRKDRTTHPPRYIVIFKS
                     KQADNLEQAFKEFTAKKLQQQERPSIRKTLAVLKEKAAARSSQQAKEKIKERGLSR"
     gene            complement(15595..15807)
                     /locus_tag="IW781_002167"
     CDS             complement(15595..15807)
                     /locus_tag="IW781_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1183159.1"
                     /translation="MKQGALIFDEQTDRYDIRFDLADYYGGLHCGETFDVMVGGRWRP
                     TRIEMAENWYLVGIHADDLSGLRVRI"
     gene            complement(15909..17054)
                     /locus_tag="IW781_002168"
     CDS             complement(15909..17054)
                     /locus_tag="IW781_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1183160.1"
                     /translation="MYFTSGSDRAFEQLMQTRPGADHFDNGEAGAPEDCGTCRFYRPH
                     WKYQFCVYEECPYQPGKLTALDGAVKFQVKGVDDEMAVFRVEKNRGYTVMSNHHLRNK
                     DLSLKAKGLLSQMLSLPEDWDFTLKGLSLINREQIDAIRAAVRELEQAGYIVRSRERD
                     SQGRLRGADYIIYEQPQPVPDSPALENPTLDNPTQEKPTQEKPTQLNKDRLSKEKSIT
                     DGSNTDSIPILSPPSPLGEEAAALPERKGTGAKSQSAVEIYREIIKDNIEYEYLCQYA
                     KGIDRDMLDEIVDLLVETVCSARKTIRIAGDDYPAELVKSKLMKLNSSHIEFVFDCIS
                     KNTTEIRNIKKYLLAVLFNAPSTINGYYTALVAHDMNTGSWGTPQNR"
     gene            complement(17315..17704)
                     /locus_tag="IW781_002169"
                     /pseudo
     CDS             complement(17315..17704)
                     /locus_tag="IW781_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3546 a         4993 c         4600 g         4568 t
ORIGIN      
        1 taataagcgg ctgatattca attttgaaag tttgggttag attgattgaa gctggggaca
       61 gtctaacaaa ttaaagcttt ctacttcttc aacatcaata actttgatgc ctcgttcttt
      121 taatgccttt gcaaacaatc ccattccaga aatcagcttt ccggtaaagc taccatcata
      181 tatttggttt actccgcagg taggacttct ggattgtagt atagctaact caatatcttc
      241 atgttgaatt tgggataatg ccattgatac tgctcgctta tattccaaat cgacatcatt
      301 tccgttttcg tctgttacta caccattcac aatctctgca caaggtctgg gagtagtcat
      361 acctgctaat tgctctggac aaatgcaaat aatttcttta tcttttaagt agttgatagc
      421 tgccagattt ttgttatttt ttccattgta tttacaatcc actcccataa tacaagcact
      481 aactaatatt ttcacactta tcagtcgagt agacagcctc gacactcctt tcacataatt
      541 tctgctgtga agggattgtc tactgcccct cacagaaccg gacgtgcagt tttcccgcat
      601 ccggctcttc aagcaatctt cggttgccag ccccaaatat ctgtggtcag atgcggctct
      661 ttgacatagt agttccagat tcgtagaaac ctgttatact tcatggatcg cttctggtgg
      721 cggcggttca gcactctgta ggtggagtat ttcaggtatt tccagaaact ctgtatagca
      781 tgaaaattcc cgttcacccc atagtagttg tagtgtcctc ggaggataga agctatcgtt
      841 ctcatcgtct ccgctactgg cctgtgcatc tgttccctga gccatatctt ggccgcctgt
      901 cgcttcgcct tcagcttctt cttgcttgtg cgaactccaa gccgatactt tcctgaccga
      961 gttttggcgt tgaagaaggt aaagccgagg aagtcaaaat cctcctttgt ccctttgaac
     1021 cgtccaatgg gcagtattcg cgtcttgtcc tccgccactt ccagtgaaaa tttacccagc
     1081 cgctttttca gcgtatccat aacctttcgg gcctcattct catactgaaa tagaatcaaa
     1141 aagtcgtctg cgtaccgcac atagtagact tcgccccgca gatgcggctt aatagctttc
     1201 tccatccaca gatccagcac atagtgcaga tacacgttcg ccagcaccgg tgaaatcagt
     1261 ccgccctgcg gcgtccctct atcactggcc agacgttttc catcctccat gatccccgca
     1321 atcagaaatc gcttcacgta tcgcagatag ttcttgtctt gtatgtcatg gaccaagaac
     1381 ctcatcagcc aatcgtggtc aacattgtca aagaatccct taatgtcggc ctccagcaca
     1441 taattgactt tcctcgtcat aatggtctgg ttgatgtatc tcaccacatc gtgcgcaccc
     1501 ttaccaggtc ggaatccata ggaacatccc aggaagcgag gctcatagac ttcgttcagt
     1561 actttcgcca tcacgccctg caccagccgg tcttcatagg ccggtattcc cagcggcctc
     1621 aatttcccat tggccttggg aatatatgtc cgccgcaccg ggagcggctt gtactggaat
     1681 ttcctcatcc gctctaccag cgccgcgatt cgctccgggg cattctcgtc ataggctgct
     1741 ttgtctattc cgtctacacc ggttgccttt ttcctgctct gctttcgatg ttccgccatc
     1801 agtgtcctct cgtttacatt gtgcatgaga ttctgcactg ccggatactt tgctgactgc
     1861 tcccttattc ccttcggttc ctgttccatc cgttctgacc tcctgtgtcc ggatgcttct
     1921 tcctccggga ccgattactt gctgccccct ttgctccacc ggctttcacc ggtttcctcg
     1981 ctactatgag gcagtccgac ttctgacagg ccttcggggt ctctccattt tcaagacttc
     2041 aattcccctt accatctcgt ggagcctgtc agatctccca ggtatacagg tactaccttt
     2101 gtacgctcgc cacgctctat tgaccccgac ggaatctccc caaccttgcg cttgcgggtg
     2161 gttcttgctg cctgcgacct tgaggacggt atcggcttcc gctctttata tcttacgagg
     2221 ctgtatcgct tcacggtttc ccattgcggc tctcgcactc ctctgcctac gcttaaacct
     2281 cacctcgcgg cttcggctcc aaggctgagt accggctgct cgctacgctt tgccgggtct
     2341 ggtctttcac cagactgtat tacctgcacc gaactggcgc accctcaaac ggatattatc
     2401 gttggcttta ttatactatg acatggtgta tcttggaata gatgaattgt aaatttgctg
     2461 gatacaaggg ggttggggct tccccaaaga gaaaatcccc ggcccggcag gtgcgccggg
     2521 tcagggattt tgccgggagt gtggctacac tccctatgct tgctgttttt agttcttgtc
     2581 cctctgttcc acgcggtagt tcacatactt gccgccggta tcggccaaaa gcaccgtccc
     2641 gtcataggtc ttaccggttt tcatggagcg caggcccttt acctttgcct ttccatcttt
     2701 gagcagcgca gcggcgatct tcggggtgaa cgccttgccc cgttcctcaa aaaatcggtc
     2761 attcttccac atgacaaact ggcagcttcg gtttccgcag tagtagttct ttttgccctc
     2821 gtagacgctt tccccgcagc gcggacattt gccgataacc acacgctcgg tttgaaacaa
     2881 cttctgcgcg tcctcgctga tctgggagta gtttgcgatc aggccccttg tcatttctgc
     2941 aataccagcc atgaagccct ccgggtcggc ctgccccttg gcaatggccg tcagctccgt
     3001 ttcccactgt gccgtaatct ggggagaggt cagcacctcc ggcagcacac aggcaaggtt
     3061 gtggccgtcc ttggtgggaa gtagctgttt gcccctgcgc tccacaaagc ccatctgcac
     3121 cagcttttct aagatggagg cgcgggtggc cggggttccc aatccctgcc gctcggcgtc
     3181 ctctggcaga tcgtccgcac cggcgcgctc catagcggaa agcagggtat cttccgtgta
     3241 ggattttggc ggcgctgtga aatgctcggt gacgacggca gttaccttgt caaaggtctg
     3301 tccctcggtg atctccggca gttcccgcgc aggctccggg gcggtgtcgt cggcgtctgt
     3361 tttgaacgac gccttaaagc ggcggtcaag ctctttccag ccgggggtca ggatggtctt
     3421 tcccttggcg gtaaaggtgt tcccggcaca ggtaaatgtg gcggtgacag cctcgtacac
     3481 atggggcgcg gccacggcgc acagcagctt gcagcacaca agggacagca gtttcttttc
     3541 cccctcggtc agcccgtcaa agcctttctg cacaaattcc attgtcggga caatggcgtg
     3601 gtggtcgctt acctttttgc tgttcagcac ccggccaaac tgcggggaca ggtcaagccc
     3661 cgccgcaaag ggaagcagcg gccacaggcc ggacacaagg ctttccgccg ttggcagcat
     3721 ctcatcggtc aaatactggc tgtcagtgcg gggataggtc agcagcttct tttcataaag
     3781 ctgctgggca tagtcaaggg tctgcttggc ggtaaatcca aacaggcggt ttgcttcccg
     3841 ctgcaaggtg gtgaggtcat agaggcgggg cggttgctcc gtctttttct cccgtgttac
     3901 cgacacacaa acggcctgcg ccgtttcaca ggcggctttc agggccttgg cgtcgtctgc
     3961 gtttcccatg cgttcactgg ccgcctccat gccgcccgcc gcgatctgga cgatgtggta
     4021 tttctctttc tgaaagctgc tgatcttggc ctcccggtca accagcattt ttaaggtcgg
     4081 cgtctggaca cggcccacgg tcagggtctt gtggtagagg atggaaaaaa gtcttgtcgc
     4141 gttgatcccc accagccaat ccgcctgcgc ccggcaaagg gccgactgat acaggttgtc
     4201 gtagtcagtt cccggtttca gatgggcaaa accgtcttga atggccgcgt cctccatgct
     4261 ggaaatccaa aggcgctgaa acggtttctt gcagcccgcc atctggtaga caaaacggaa
     4321 aatcaattcc ccctcgcgcc cggcgtcggt ggcacacaca aggccggtga catctgggcg
     4381 ctccatcaaa gaacgcaggg tgtcaaactg ctttttctta tcgtcgggaa tgatgtattg
     4441 ccattcctgc ggcagaatcg gcaggtcgtc atagcgccat tttttgaaac gctcgtcata
     4501 agtcccggcg tcggcaaggg aaacaaggtg gccgatacac cagctcacca gatagccgtt
     4561 tccctccatg tagccgtcct gtttcttgtc cgcgccgatc acgcccgcaa tgctgcgggc
     4621 gacgcttggt ttctctgcaa ttactaactg atagctcata cttcgtcctc cgtatttcca
     4681 tcttcacttg tcatatcctc gttcggctcc ggggtttcgt cctcgtcgtc ctcgtcctcg
     4741 ccaaagtcat actcagccag atcgtcattt cctgtggtat cggctttttc ctttttgaat
     4801 ttcagaaagt agagggctgc gccgccgccc aacagcacca ccacaaagaa caccacaagg
     4861 ccgcccatgc cgcttggctt ttcctcctgc agcttttctt cctccggctg gggcttcggc
     4921 tccggcccgt tgcaggcggt caggttgttt ttacagaccg ggcaggccgt attgacggcc
     4981 ccggcttgac acttggccgt acagttgcag acctctggct cttttttccc gtcctccaac
     5041 aaggcttgca ggtcggcgtc gtccacttgg ttgaggaaat gcaccgccgt ttccttgtcc
     5101 tcgttggccc ggtcgatcag gatgtaaaaa taattcccgg ccttggtagt gacggtgata
     5161 agctgcttat ccccgaaaaa atcatccacc agcgcggcgt tgccctgcag ggtgacaggt
     5221 acgccctccg gctccatgcc gccggtggtg ggttcttcgg tttcctccgg cacttctgcg
     5281 ggggcctcgg tggtttccgg cagcggcacc gggtcagggt cagcgccccc ggcgtaggcg
     5341 gtaacggtcg tggtcgccat acagagcgca gcggccacag ccagcgtcaa agtgcggaac
     5401 agtttagatt tcttcatggg cgctgtcctc ctgttcaaag tccgggatcg gggtcggggc
     5461 cgcctgcaac gcgccgccgc gcacaaaggc ggcgaactcc tgcggggtca tgttgaggcc
     5521 gcgcacaagc tggacgatct gcaagttttc ctgttcggtc ttggccgctt ccagctcccg
     5581 aagctgcttt tgcagctccg cgatcttgcc cttggttttc tcaatatcct tttcaatgcg
     5641 ttccaatttg ttcattgcca tagtcaaaaa ctcctttctg gcgtttaagg caaacgccca
     5701 taacaataaa aatgctgctg ccagtagctt gaattgaggt ttgtgtagga aatggggtcg
     5761 ccgcagtgca gcatgaccga atttcccaca tacagcccca catgggacat cccggcagtg
     5821 tcataagtgc cgacgaaaaa tacaaggtcg ccgggcttgg cctgctccgg ggaaacgggg
     5881 gtgcagatgt tgtaaagccc ctgcgccgtc tggcgtccca cattccagcc ggaatgattg
     5941 ataacccagc taatgaagcc ggaacaatca aagctggtgc ttggggagct gccgccccac
     6001 acatagggat agcccacata cttttccgcc tccgcgatca tggcggcaaa ggcttcgtcc
     6061 tttagcgcct ccggggggat ttcatagtag gtcggctctt tcggggtgga ggcgtgggga
     6121 tagctgccgt tgggaaacag gtctggccgg ttgcccaaag tctgcatata ggcagcataa
     6181 aagcccatct gttcctcggt gagaatgtag atgggcaggt gggacaggtc aaagttttcc
     6241 agcttcacat tgcaaatata gtaggtgtag gggacttcca cggtgtaggt gtttccctcg
     6301 ctgtccgtcc tcgtttcggt gcggtagcgg gtttccgttt cgatggtctg ggtcaggatg
     6361 tactgctttt caaagagcat ggcaaggtcg ccctgtacct cccccaaagt gaacacgccg
     6421 ttatgcagcg cggagaggat ggaggtcagc acataggggt catgcttgat ctcgtccaga
     6481 tcgaaacggt attcgtcata gccggggtgc aggctttcgt agttatcaag ttcgtgctgc
     6541 aagtctgctt ccagcgcggc ataggcggct tcggctccca gcatatcgct gtcctcggag
     6601 agataggagg tggcggcaag gcccgtcccg gtgctgccaa acatggcggt gcaggattgc
     6661 aggccgccca tcaccagcag gagcataagg cccacgccga cgacgatcag cgcccctttc
     6721 cagtgccggg cggcaaagga cgcggcctgt ctgcttttct ctgcggcttt cttggctgcg
     6781 gcagccgtgg tcttggcggt gcctgccgcc ccctgtgcgg tctggcccgc cgcccttgct
     6841 gccttggcgt attcccgctt gatgtgctgt ttctgccaga agcgggaaac ggggttcttg
     6901 gccgcctgcg ccagctccgg gttgtcccgc agggattttt gatacgcaaa ctcggcgttg
     6961 gcggcgatgg actttctctc ggccttggct gcggcccggt agggtttgag cttgtggcgg
     7021 tggacgccgc tgcggatcat gcggcccgcc tgccgttccg ccagttcctc ccccttgtgg
     7081 ccgccctcca cgcccacatt ttcgtgttct acctcatgga tttttccgtg gaggtaaagc
     7141 ccggcttcct gtatgggacg gctggccggg tttggtttga gctggggagg agctttctcc
     7201 accttgtcaa aatacagcct gcttttggcc ttgcccttgg cttcgtcata gacggtttcc
     7261 ttggtgacga cgcgcttttt cggcagggct tctttggccg cttccagctg atccgcccgt
     7321 ttttccgctt ttttgatata cggggccagc tccggggagg cccgttcctc ggcggtaaac
     7381 tgcaaccggg aaacggggcc ggtggcccgc accacggttt ccccggcgtc cttggccgct
     7441 ttctttgcct tgtggcggtc gtgcagttcg tccgcccgtt ccagtatttt ttccgccgcg
     7501 ccctctggct gggcggtgta ttcctgctcg gcctcccggc tggacacatt gacgctttcg
     7561 ccggtggtct ggttatccag cgtcaggccg tcgcgggtca tgtgctgcgt taccttgtcg
     7621 cggggtttta agggttccat atccgatcac ctccttgctg tctgttccgg cagcgcccgg
     7681 tttcaatctc tgcaatatag cgtaggatgg ggtacacctg cgggggaaca actgcgtttc
     7741 ccaaagtctt taaccgcttt gccacattcg ccggcgggtc gctgattaag tacgggattt
     7801 gcgccggttc tttggcccac atggaatgtc cgtccatctc cgggggaatc ccatcagcca
     7861 ttccacccag tcggggttca gtttcacatc ccccgtgtcg ctggcaaggg gcgtgggaag
     7921 cagcatggcc gatgatgaaa gttcttttgc gctcatgcca tgcgccgacg gcgacagcag
     7981 gaagtacgaa aaccctaacg gcgtagcctg cctgctccaa gtcaaaaacc gtcttgtcga
     8041 gccccagatg gatgaagcca gcaacatttt ctccaagcac ccaagagggg tgcagttccc
     8101 ggataacgcg gagcatttcg ggccataaat accgttcgtc ctcaaatccc cgttgtttcc
     8161 cggcgctgga aaagggctgg caggggaatc ccccggaaat aagtgtggtg gtttctcgtc
     8221 ctgttttttc aaaaaatgcc tcctttgtca ctgtggtaat gtcccgaaat tttggtacct
     8281 gcggccagtg ctttttcaaa acttccatcg ggaaatccgc ccattcgcac tggcatacgg
     8341 tggtaaaccc tgcggcttcc gccgcaaggt cgatcccgcc gatcccggaa aacaggctga
     8401 aatgtgttaa ggcgctcata ccgtcgcccc ctcgcctaac cgggtcgtca tgatctggta
     8461 gagctgggtg tttttcggga tggggttctt aaagggcagg atcacatttt cgtaaatgag
     8521 aagtccctcg ccgggttcgg aattgtccac ataccgtaat tgctggggcg aaatgttcag
     8581 ccgttccgca aggattttgc ggtcgccgct ggcctgattg agcaggcaga taaagtcgct
     8641 gttttccaaa atgttctcaa tttccgggga ggaaagcagg tcttttacat tctgcgttgc
     8701 acctgtggga atcccgcccc acttacgaaa acgcttccaa atctcacagg aaaaagccgc
     8761 cgtctgttcc tcacgcaaaa gcaggtggaa ctcatcacag aaataccatg tcgccttgcc
     8821 cttgctgcgg ttttgggtga cgcgccccca aatctggtct tggacaatga gcataccgat
     8881 tttctttagc tgcttgccca gctccttgat gtcaaaggcc acaatgcggt tctggatgtc
     8941 gatgttggtt ctgtggttga acacatttag gctgccagac acatacaggt caagggcctg
     9001 cgccacccgg tcagcctccg ggatatgctg gtctaacagg gctttatgca ggtctgcaag
     9061 gatgggcata ttttccggct tggggtctgc cagataaggc cggtagatca cctgtaccgc
     9121 ccggtcgatc accgtctttt caatggcttc cagcccgttc ttgccgccca tgacaagctc
     9181 acagaacgac agcacaaagt cggatttcaa ggccagcggg ttttcatcct cggagtaatt
     9241 caggttgatg tccagcgggt tcacatagtt tttgctggta ggcgacagct tcaccacctg
     9301 cccatgcagc cgcttcacaa gggggtaata ctcggcttct ggatcacaga tatacacatc
     9361 gtccggggtg cagaggaaca cgctcaaaat ctcggattta cagctcatac ttttgccgct
     9421 tcccggtgtc cccagcttta gcccgttggg acacctcgcc cgtttgcggt cgagcataat
     9481 catgttgccg gtcttggcgt tgatcccgta atacatggca tcgccgccct gaaaaagctc
     9541 ctgcgtcaca aaggggacga acacggccac gctggaagtc gtcagggagc gttcaatctg
     9601 gatgtggctg gcccccagcg gcaggctgct cataagcccc tgttcctgct ggtagtccag
     9661 ccgcacaagg gaacagttgt atttctgtgc aataccggca gcccagaaca catcgttttc
     9721 cagcttttgc cgggtgtctg cggtgttcag caccaaaaag gtaagctgga acatccgttc
     9781 attccggctc tgcaaggtct ttaacagctc cttggcgtcc tgcccgtagg tggcaaggtc
     9841 gctgggaagt atatccatgt cgtaaccact tcttaccgcc cgtttctgtt cctgaatctt
     9901 catggcgtca aggtcggtga tcttgcgctt gaccgtctta atggcgtcgc tctggtcgat
     9961 ggcctgcaca tggaggttca ccacaatgcc gttttctgtg ttgaggaagt cggccagcat
    10021 ttcatccgac agctccgggg agaggatttg caggaagctc accgccccgt atttgccgcc
    10081 cagcccgaac atccgggcat tgccgaagcg gaacgaggac ggggcgataa agtccttggt
    10141 ggaaagcccc gacggggcaa gccacttcca gtcaaaggag aacggttccc cgtccgggtg
    10201 gaaaatgctg tgcatgactt ccagccgctg cttggcgtcc agtccccatg cggagtcccc
    10261 catgctcttg aaatagccta agaggtcggt ttcaatccgg cgaagtctgg cgcgggccat
    10321 tttcagattg tccgcctcaa tggtataggt cataaatttt cgcttcatca ggccgttgtt
    10381 cccatttaca agctgctctt gcaaaatgcc ggaatactcg ctgcggatgt cgtcgaagtc
    10441 gtccccctgc gcccggattt caaagctctt ggcgtactgc ttggggtcga tcttgcggtt
    10501 gataaaggaa aactgcacat gaatggaggc gtccagatag ttgtagaggt cgcacaggtt
    10561 ttcaaatatg gcggtcttgg tgtctgcctg cgccagttgg tagctgatgt ccgtaaactc
    10621 aatgcacttg gaaaaggtgt ggggcgtcac ccggcacaca ccgtcctgac acatggcctc
    10681 atagggaata ctggcctgcg ccgaatgggg cttgccgtcg cccttatact ttcggatgac
    10741 ggcctgaatc tctttctttt cggcgcgggt gagcttgacc ggcgcttttg cgctcgtttc
    10801 gttcctttgc tttttggaca atggcgttga cctccttttc catttgggat tgccgcataa
    10861 gggcggtata agcgttgttg gtctggtagg gcctctcctt tggctggatg aagcagcact
    10921 cgacgatctg gcgcaccacc acttcaaggg gctggccgtg tttttcgtaa agcgccagca
    10981 gaaagcccgg cagcatggaa aacaccataa tgagcgccgc cgcgctggtc gggacgcggc
    11041 cccggatcaa aaagaaaagc gggacgccta caagcagcgc accgccaaaa caaatgagct
    11101 ggcgtttcgt cagcccgaat accaccttgg atttgacctt ggttaaatcc ttggggacag
    11161 ttacatatgc cataaaaagt cacctcgttt ctggtacgga cagggaaaaa tcgctgtcca
    11221 cttcattgcc ccacacatcc cagccgggga ttttctgtct ggcaaacagt tctgcgcggg
    11281 gcaggtcgcc cgcaagggct atgattttct cgcgggtaat gtccggtttc ttgctgtgtt
    11341 cctccaccgg agaaatgata aactgatgga cgcttttgga gtaacgcttg ggatggcccc
    11401 gcttggcaag caggcagatt tccgcgtttc ctctcgtcca gtagcccagc ccgtaaaacc
    11461 atgtggggct tttgcggttt aatttcagcc agacaaaggc cacggtctta aaggtgaagc
    11521 cccacgcctt aatcagacgc agcgcctccg ggagctgtgg gaaagttgcc cacaaaaaca
    11581 agaggcagtc tttttccgcc agcgtttcca ccggcagggc gcacagctcg tcaatgctca
    11641 tggtgggata gtgcttttct gctgcaccct gtactgcttt ctgttcatat cgccacggcg
    11701 ggtcggcgta aaggatgttg taggttttct tttgtgtttc tttgatggtt ggtattcctc
    11761 ctgtctgtca atgtgcggta aaaacggctt tcgatatgct gccggtcttg aacagacaga
    11821 agcaaagcaa aacggtatag cccatacagc cccagattgc gccggatatg tcgtcggcgg
    11881 ttccgatctg ctggatcagc accgcataaa tcccgacaac caccatgatt aaaaatgctt
    11941 ggaagcccag cgcaagcagg gatttcaagt agttctggcc catgccgcgc cactctgcat
    12001 tgcccagcgt ggcaaggggg atcggcccca atgcggtcac ggcgtaaatt tcaatcattc
    12061 ttccataggt cacaaggaaa atgcagatgg aaagaatgtt catcgtcagc cccacaacaa
    12121 gggtctggaa ccataggccc agaagcggcc cgatgtccat tgcttcaagg cggctttcca
    12181 gatcagggag cagcgcgtca aaatcaatgc tggtatctcc gataatcacc ccggcgctct
    12241 ggttgacgac ttgctgggta gcgtcaaaca cgcccatgac gatattccat gtgttcgtca
    12301 caatgaggat ggcaaaggcg cttttgaaaa tccagcggaa gaacatggag ctgtcgaagt
    12361 cgtgcatatt gttcttttcc acgatcatgt cgatcagctc atagcacatc acaagggcga
    12421 gaatggccgc cgcaatgggg acgatcaccg tttcggacag gttttgcagc atggaaaaaa
    12481 tgccgccgtt ccagtcctgc ggggtactgc ccacatccga cgcgatttcc ccgactttgg
    12541 tattgaccgt gttaaacagg ccggacaggt tgcccatgat accgtcgatc aggattcctt
    12601 tgatccattc cccgattaaa tcaccgatca tgtggggaca cctcctttcc cgcgccctcc
    12661 cagatcagag agggcgcgga gggttttcta aggcacatgg ggttatccca gcagcccgga
    12721 cagcagcgga acaagggtca tgccgatgag ggcaacgccg cctccggcga caagctgctt
    12781 gattccctgt gacttgctcg ccgggttgtc cgccccgtat ccctccaaaa gattgacgcc
    12841 gccccatacg caaagcgcgc cgccgaggcc cacaacgatg gtctgcaaag tatcaactgc
    12901 actattgaaa aattccataa agttacctcc ataaattcag attgtgatgg tgtttgggtg
    12961 caaaaaagcc gccgtacaca gcggcagcct cacaatccgc agacggcccg gagttactgg
    13021 ccgtcccgcc atttcagcac agccagcgaa aaaatttcgc cccatgcttc acacaattca
    13081 cggtttactt ccgcctccat ctgtttcaaa ccggcgtcgt caggcagttc aaaatcgtgt
    13141 tcaatcaacc accgcaatgc aacggcagcg gaaatgatag cccggtagat ttcccgctcc
    13201 tgcttgaaat aatccgcaaa tgtccattcg tccgagtgtc ccatcaagtt acgctccttt
    13261 ctctatgacc gctttattct gcggcaaaat cttcctccga cagttcaatg tcgtaaaggt
    13321 caaagggttc gtcctccgtt accacgcgcc gctttttccg gcgcagggac gacagatagc
    13381 tgtccacatc aaaggtattc tttttgtcag cgtccgagag gtattgatag cgggggtggc
    13441 gggtaatgtc gtacttctcc gagaagaacg gcctcacgcc gcgcacctgc aaaatacact
    13501 tgcctccgtc catcgttgca atttcgtctt ggctcatcag ctcctttccg agtttttggt
    13561 agttcagtcc gtgggaggtc tgtgcgcccc ggttctcgct ctggttgtag ctgtcaatgg
    13621 tttccttgcc cagcacctcc gcaatctcct tggcgttctt gcccctgcca ctcaggaaca
    13681 gcgtacagtc gcagttgtcg gaaatgattt ccgccgcgtc cttgtaaatg gcctttagct
    13741 gcgactggct ctgtaaaata atagaagccg agatttcccg gcttcggatg gtggcgatca
    13801 gcttgtcaaa gtttggaatc tggccgatgt ttgcgaactc gtctaaaatc agccgcacat
    13861 ggacgggcag ccgcccgccg tatttgtcgt cggcccggtc gcataacagg ttgataagct
    13921 ggctttgcac cattgccaga atgaaattaa aggtggtgtc cgtgtccgac ataatcagga
    13981 acagggctgt ttttctatcc ccgatggtgt caagttccag ttcgtcgtcc tccatcagct
    14041 cccgcacctc ccggatgtca aagggcgcta atctggctcc gcagcttatc aaaatggagc
    14101 tgcgggtctt gcccgccgac agcaggaatt tacggtactg gcgcaccgca aagtgttccg
    14161 ggtctttttc ttccagccgt tcaaacatct ggtctaccgg ggattgaaat tccgggtcgt
    14221 cctcgcgggc ttcgctggca tttatcattt caagcaacgt cgtgaaattc atttcatcgt
    14281 ccggcgcttc gtaccagata tagccaatga gggccgaata cagcagccgt tccgatttta
    14341 tccaaaaatc ttccgcgctc ttttcgccct cgcccttggt gttgcagatc agcgtgttta
    14401 ccagcttcaa aatatccttt tccgaatgga tgtagcggaa cgggttgtaa cgcatactct
    14461 tggaaaagtt aatggtattc agcactttta cccggtagcc gcaccgcaca aggagctggc
    14521 ccacctcgcc gatcagactt cctttcgggt cagtaatcac gaatgaggta gggtagtcct
    14581 tggacacgca ctgcataagg ttgggcttca caaagaaccg ggtcttgccg ctgccggaac
    14641 cgccgatcac cagtacattt ttgttccggg cggtcttggg gtctttgggc cggttgttca
    14701 tggtgaggcg ttccgtctgg gtcaggagga tgttgttgtc aaacaccggg tcgatgtacg
    14761 gggctatatc tttggggcct ccccaacggg cgctaccgta ctcgacgccc cggcggtatt
    14821 tcttggcgtt cttgcttttg cagtacacca tcagccgcag tgccaccgca ccggccatgc
    14881 ccaccgccag atcgaacagg tggaagctgg ggacggcgct ttcaaaggca agggaaaagc
    14941 cgtccgcaag gtgcagcagt ttttccgaca agtccgcccc agccgccagc cggtaggtct
    15001 gtcccagctt gccgaacagg taaacaaaca gcaggtaagg caggttggca attatcagct
    15061 ttttcatttc cggcttcatc gtgacagccc cctttccttg attttctcct tggcctgctg
    15121 gctgcttctg gccgctgcct tttctttcag gacggcaagg gtctttcgga tggaggggcg
    15181 ttcctgctgt tggagcttct tggccgtaaa ctccttaaac gcctgttcca gattgtccgc
    15241 ctgcttggat ttgaagatca cgatataccg gggcggatgg gtggtgcggt ctttccgcag
    15301 ggtaaaatca atgtcgtatt tcttggcaca gggcttaaac aggccgatgt tggcgtcggt
    15361 gatctcaatg ttggacaggg cggaaccgtc ctttttgagc tgccgtaagc tctgctggcc
    15421 gtgatgggcc ttgcccgtcc ctttctgccg ggcggccaga tattttcgga tggccgcttg
    15481 cagcacttgg gcggtgagct tgcccgtctt aaccgctaac gctatggttt tctggtctac
    15541 ttcttcctgc aactgctatt cctcctttgg aaagtcagca ggcaggcgga tggcttaaat
    15601 ccgcacccgc aggccggaga ggtcgtcggc atggattccc accagatacc agttctcggc
    15661 catttcaatg cgggtcggcc tccatctgcc gcccaccatc acatcaaaag tttctccgca
    15721 gtgcaggccg ccgtaatagt ccgcaaggtc aaagcggatg tcgtagcggt ctgtctgctc
    15781 gtcaaaaatc aaagcaccct gtttcatagg gtcgtcctcc tttctcgtcg tcacaagctc
    15841 catatcgctt gtttccacgc aagcgtgaaa agcttgctca tttcgctgtt cctcctctcc
    15901 ccacaaaatc accgattttg cggggttccc caactgcctg tgttcatgtc atgggccacc
    15961 agcgccgtgt aatagccgtt gatggtgctt ggggcgttga acagaaccgc cagcaggtat
    16021 tttttgatgt tccgtatttc cgtggtgttt ttgctgatac agtcaaagac aaactcaatg
    16081 tgagaactgt tcagcttcat cagcttggat ttcaccagct cggcggggta atcgtccccg
    16141 gcaatgcgga tagtctttcg ggcgctgcat acggtttcca ccagcaagtc cacaatctcg
    16201 tccagcatat cccggtcaat ccccttggcg tactggcaaa ggtattcata ctcgatgttg
    16261 tccttgatga tctcccgata aatctctacg gcgctctgtg atttcgctcc cgttcctttc
    16321 cgttccggca gcgcagccgc ttcttccccc aaaggagagg gaggggaaag gataggaatg
    16381 gaatcggtat ttgatccatc tgtaattgat ttctctttac ttaatctatc tttatttaat
    16441 tgcgttggtt tttcctgcgt aggcttttcc tgcgttggat tatccaatgt tggattttcc
    16501 aatgcaggtg aatccggcac aggctggggc tgttcgtaaa tgatgtaatc cgccccgcgc
    16561 aggcggcctt ggctgtcacg ctcacgggaa cgcacgatat agcccgcctg ttccagctcc
    16621 cggacagccg cccggatcgc gtcgatctgt tcccggttga taagggacaa gcctttcagc
    16681 gtaaagtccc aatcctccgg caaggacagc atttgcgaca gcagcccctt ggctttcagg
    16741 gacaagtcct tgttccgcag gtggtggttg gacatcaccg tgtagccccg gtttttctcc
    16801 actcgaaaaa ctgccatctc gtcgtccact cctttcactt gaaatttcac cgcgccgtca
    16861 agggcggtga gcttgcccgg ctggtaggga cattcctcat acacacaaaa ctggtacttc
    16921 cagtgcgggc ggtagaaacg gcaggtaccg cagtcctccg gcgctccggc ctcgccgttg
    16981 tcaaaatgat ctgcgcccgg cctcgtctgc atgagctgtt caaaggcccg gtcgctgccg
    17041 gaagtaaagt acataggcgg tcgcctcctt tctggttttg ggcgcaaaaa aagcggcgtc
    17101 ctgatctccc caaaggggaa aagagaacgc cgccgtctgc ggtgtcgtat tcagttttcg
    17161 atgtgtcact atttgggtag tgggcttcac gggaaagtag aagcgtgtca aagctcaatc
    17221 cgaaagtagt aaatcggtag taaattcagt tcctatatcc cgtgaaatgc ccgtatttcc
    17281 gggctttttt gagataatca gagttgttaa agaaaacatc aaatacatgt ccttgaaatt
    17341 gtatcgaggc agctggattt agtttaaatt gaggaaactt taccacagct gattcaaaac
    17401 acttgtcaca tttttttatt tttttacctt tgtaaaaata ttcgattgaa tattctattt
    17461 ggcacgactc acttggcagt atatgtatgt caatattttt catctgtttc ataggtattt
    17521 caaaatctaa actattaaaa acatataatt ccctgtaatt atttacatct aactctacta
    17581 aatttatctt tggaacaata gatcctccac ccattccacc ataataaaaa aaatcttgtt
    17641 ctctatcaaa atagcacata ttaagctcag tttgtttatc atcctcaata attttataaa
    17701 tcaaacc
//
LOCUS       DACEOM010000064        17587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEOM010000064 DACEOM010000000
VERSION     DACEOM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.065"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            363..731
                     /locus_tag="IW781_002170"
     CDS             363..731
                     /locus_tag="IW781_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1183161.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IW781_002171"
     CDS             911..1852
                     /locus_tag="IW781_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1183162.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IW781_002172"
     CDS             1921..2652
                     /locus_tag="IW781_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1183163.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IW781_002173"
     CDS             2796..4976
                     /locus_tag="IW781_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1183164.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IW781_002174"
     CDS             4996..5478
                     /locus_tag="IW781_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1183165.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IW781_002175"
     CDS             5494..6255
                     /locus_tag="IW781_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1183166.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IW781_002176"
     CDS             6386..7720
                     /locus_tag="IW781_002176"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1183167.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IW781_002177"
     CDS             8027..8512
                     /locus_tag="IW781_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1183168.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IW781_002178"
     CDS             8505..9188
                     /locus_tag="IW781_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1183169.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IW781_002179"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IW781_002179"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1183170.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IW781_002180"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IW781_002180"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1183171.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IW781_002181"
     CDS             12804..13433
                     /locus_tag="IW781_002181"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1183172.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IW781_002182"
     CDS             13485..13817
                     /locus_tag="IW781_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1183173.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IW781_002183"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IW781_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1183174.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IW781_002184"
     CDS             14643..16016
                     /locus_tag="IW781_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1183175.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IW781_002185"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IW781_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1183176.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IW781_002186"
     CDS             17071..17511
                     /locus_tag="IW781_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1183177.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5993 a         3068 c         3866 g         4660 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatggga
//
LOCUS       DACEOM010000065        17366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEOM010000065 DACEOM010000000
VERSION     DACEOM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.066"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..298)
                     /locus_tag="IW781_002187"
     CDS             complement(<1..298)
                     /locus_tag="IW781_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183178.1"
                     /translation="MQETTSISKGGIVVAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFDGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSE"
     gene            complement(273..2978)
                     /locus_tag="IW781_002188"
     CDS             complement(273..2978)
                     /locus_tag="IW781_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1183179.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPIEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLIGG
                     VPDNDYDEDKEPIEYGYLETDGVTNENRRIGKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELAALHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDFVLGNDLSLYTSTVNTQTQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAMTSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWKENKKIMEITKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILISPAVGKGFVLLGNGKATYFGDF
                     DVQGTFTVKGKEIDGGNSGGNDNGWNGQYPPEVTTDRDKRYWQIWKMAIGAGFTKQAA
                     AALLGNAQGESDANPTADEGNGAPGFGYGIWQWTDSTGATSGRVYMLNLMTKAGISDD
                     PDTITAQFKLLMWHAPNGQWIATSAYPYSWTQFMTLTDINTAAQAFVANFERPRDPHP
                     ERTTWSQEWYDKFKDLEIPASKGYIKPIADPIRVTSEFGWRTSPITGAQEFHNGIDLV
                     NGNPNTPIFASADGEVIVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKV
                     TAGQQIGLMGTTGPSTGEHLHFQFMDEFYPSSAAHFHNARDYIDF"
     gene            complement(2975..3679)
                     /locus_tag="IW781_002189"
     CDS             complement(2975..3679)
                     /locus_tag="IW781_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1183180.1"
                     /translation="MNPDEPNFIWKNRNAVIDMDCIIEVELPDIMPNKRYETYTVQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKKKLNEQPIIITESREDIFDPGDEVAKPYFEIVS
                     EGGHLSLTIGTKTLLVENTLAGVVKIDTELGKAIQEDAPLFTRGDWPTLNPGKNQLII
                     SGKYKEIKVWNRSVYL"
     gene            complement(3691..5997)
                     /locus_tag="IW781_002190"
     CDS             complement(3691..5997)
                     /locus_tag="IW781_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1183181.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFSEVDEAMDTIIQKTGATGDVADRLSTSFENVGSNTHLELQTVGEAI
                     GEVNTQFGFMDKKLEDSTDYLLKYADINNTDVSQAAIYARQSIEAYELSYDDLNDVLD
                     VTTKTSQNTGQSVDDLMKKAIDGAPQIKQLGLSFDEGVTLIGKFEQAGVDSSTMLSKM
                     SKASVVYAKDNLSLQDGLKGTIDSILNAKDETEALRIANEVFGKGSDKMVDAIKRGTF
                     TLDDLAKVAKESGGTVADTFAETEDPIDAANRAMNNAKFALADVGESVQISLLPFFEK
                     ATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGVKSFI
                     GVWQGLAGLFGISGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAVE
                     IFNFLGGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIFG
                     GIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGIGGKSFSISKIPYLAQGGH
                     LINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDAN
                     NPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            complement(6192..6656)
                     /locus_tag="IW781_002191"
     CDS             complement(6192..6656)
                     /locus_tag="IW781_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183182.1"
                     /translation="MARLSDYGINVEDLKNSATVTIQGVEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            complement(6656..7282)
                     /locus_tag="IW781_002192"
     CDS             complement(6656..7282)
                     /locus_tag="IW781_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1183183.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVVRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIAVMDEIKGMIAKSGVTFSKN
                     TAREFPYLAFGFIGNIEGGGKKAVWYPNTQLSNVIDEEYATAEDETKIDDVTANFVST
                     GLKYNNVMYASFDSNREEATMDLFGKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(7289..7654)
                     /locus_tag="IW781_002193"
     CDS             complement(7289..7654)
                     /locus_tag="IW781_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1183184.1"
                     /translation="MPSNKNGFSEISDYLGNLSRVDPKKLSLESLEEAAKFYLEQLLP
                     NIPKSLLKKKHMSEQIKVVVEEDRVKVQFEETAFYWRFTENGTTKQKAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            complement(7644..7982)
                     /locus_tag="IW781_002194"
     CDS             complement(7644..7982)
                     /locus_tag="IW781_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183185.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7972..8298)
                     /locus_tag="IW781_002195"
     CDS             complement(7972..8298)
                     /locus_tag="IW781_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1183186.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(8279..8557)
                     /locus_tag="IW781_002196"
     CDS             complement(8279..8557)
                     /locus_tag="IW781_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1183187.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(8559..9923)
                     /locus_tag="IW781_002197"
     CDS             complement(8559..9923)
                     /locus_tag="IW781_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1183188.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGKPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9936..10511)
                     /locus_tag="IW781_002198"
     CDS             complement(9936..10511)
                     /locus_tag="IW781_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ1183189.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSPTLLWD
                     DLSEVIAPGAFARTISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(10477..11664)
                     /locus_tag="IW781_002199"
     CDS             complement(10477..11664)
                     /locus_tag="IW781_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1183190.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSMIGQKNATDDSLPTNDQNLKGGDGNEEEGN
                     SHD"
     gene            complement(11728..13455)
                     /locus_tag="IW781_002200"
     CDS             complement(11728..13455)
                     /locus_tag="IW781_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1183191.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGDMVGKPFILEPWEKFIYSWIYGWVRENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGALGYAMYGEGALSVNCYACASDFAQAQYAAQPFADSILN
                     NPVLLDGTKIFKGPKGTVSSITYEYLDGDLALKNKFIVQTKNIENIEGSNPYFVCNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSS
                     FVLIYEPNKGYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(13452..13904)
                     /locus_tag="IW781_002201"
     CDS             complement(13452..13904)
                     /locus_tag="IW781_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ1183192.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWSDKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            complement(14016..14396)
                     /locus_tag="IW781_002202"
     CDS             complement(14016..14396)
                     /locus_tag="IW781_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1183193.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIIENETEDKKVFPSYFN"
     gene            complement(14393..14779)
                     /locus_tag="IW781_002203"
     CDS             complement(14393..14779)
                     /locus_tag="IW781_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183194.1"
                     /translation="MAMIKIRTTITGTEYWDSEKKKTVVVPKGQEPDFEATEEKGILS
                     DGKTFVAVNGELITNSSEILDSDGNTAADFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREAE"
     gene            complement(14760..15065)
                     /locus_tag="IW781_002204"
     CDS             complement(14760..15065)
                     /locus_tag="IW781_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183195.1"
                     /translation="MNDFHEAMLMANVPTNMGDVYKKAIEAENSPTGLRNNWNGTYAN
                     VQVDKDNRGIAMDVLVISLLSHTLPNLQETVEWYERMGAKVISTNYKGENQNGNDQN"
     gene            complement(15370..15513)
                     /locus_tag="IW781_002205"
     CDS             complement(15370..15513)
                     /locus_tag="IW781_002205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183196.1"
                     /translation="MRIGNVYLISKVPYQKLLYSCKVRFIASDLRNLRKQFILSDADE
                     LAD"
     gene            complement(15838..16305)
                     /locus_tag="IW781_002206"
     CDS             complement(15838..16305)
                     /locus_tag="IW781_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAQ1183197.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTANHDFEAERMLIDKEEAQEEFEYLHKLFIRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(16379..16819)
                     /locus_tag="IW781_002207"
     CDS             complement(16379..16819)
                     /locus_tag="IW781_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1183198.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(16849..17142)
                     /locus_tag="IW781_002208"
     CDS             complement(16849..17142)
                     /locus_tag="IW781_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183199.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIKS"
     gene            complement(17142..>17366)
                     /locus_tag="IW781_002209"
     CDS             complement(17142..>17366)
                     /locus_tag="IW781_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183200.1"
                     /translation="IFEGDVVSVSVRNGFDYLDNKVCIVKNSIGHSGLVCATVDEDLE
                     YRIFNTELFEEYTYEVIGNIYENSELLEEK"
BASE COUNT         4769 a         3827 c         2825 g         5945 t
ORIGIN      
        1 gctcgcttaa cgtgtaaaca agccttgcct gtgctggact gtattcctgt actttttgtt
       61 caacaggttg gttttcgcca aattttgttg tattaataaa aaatggcact aggccatcga
      121 atgtttttag tttgccatgt tccaccactt caacaacgaa cttttgtgtt aaaacatccc
      181 cttgccgaat tcgaaccaaa tttattccgt tgtttggttc ggtggtggat aggaccattt
      241 tatgctgcgt ttctgccacg actatccctc ctttagaaat cgatgtagtc tcttgcattg
      301 tgaaaatgcg ctgccgatga tggataaaac tcatccataa attgaaaatg taaatgttct
      361 cctgttgacg gtccagtagt gcccatcagt ccaatttgct gaccagcagt taccttctga
      421 ccttttgaga cgtctactcg gctttgatgt gcgtagcctg tatacattcc atcagcgtgt
      481 ttaatcactg tccaatttcc ataccagtca aagtaatttg catcacctgc aacaatcact
      541 tcgccatctg ctgatgcaaa aataggtgta ttaggatttc catttacaag gtcaataccg
      601 ttatgaaatt cttgtgcgcc tgtaattgga gaagtgcgcc agccaaattc actcgtcact
      661 ctgattggat ctgcaattgg ttttatatac ccttttgatg caggaatttc caaatctttg
      721 aacttgtcat accattcttg tgaccacgtt gttcgttctg gatgtggatc gcgtggacgt
      781 tcaaagttag ctacgaatgc ttgcgctgct gtgttgatat cggtcaacgt cataaactgt
      841 gtccacgaat aaggataagc gctggttgca atccattgtc cattaggggc atgccacatc
      901 aacaatttga actgcgccgt gatcgtgtct ggatcatcac tgatgccagc ctttgtcatt
      961 aagttgagca tatagacacg tccgctagtt gcacctgtgg aatccgtcca ttgccatata
     1021 ccatatccga accctggtgc gccattgccc tcatcagcgg ttggattagc atctgattct
     1081 ccttgagcat ttccaagtaa ggctgcagca gcttgtttag taaagccagc acctattgcc
     1141 attttccaga tctgccaata acgtttatcc ctgtcagtag ttacttctgg tggatattgt
     1201 ccattccaac cgttgtcatt tccgcctgag ttaccaccat caatttcttt ccctttcacc
     1261 gtgaaagttc cttgaacatc aaagtcacca aaataagttg cttttccgtt tcctaaaagg
     1321 acaaatccct ttcctactgc tggagaaatc aaaatatact ttccgtcgcc atttgtacgt
     1381 attaccaacg aattgtcttc taacggcgta ggagtagatg cgcctggaaa agggttgccc
     1441 gcagaatcgg ttgttccaat agttccaatt gattccttag tattccagaa ctccattcct
     1501 ttttttgtga tttccatgat ttttttattt tctttccaga tttgaagtgt gcctttaaca
     1561 agtttcagaa catcaccata agcattaaat gaagtttcaa atacttctgc gttgattgtg
     1621 ccgactttaa taaaatcagc aacgatctct cctttagaag tcattgcaat accaaaggga
     1681 ccatttactc cattatctga atatcctaga ccgtttaaat tccagcgcca cacacgcttc
     1741 gcattcgcta catttggagt atccatgata agaatctctg atggagcttt ttctggacga
     1801 aaaacgacat gtccgccgga attgcctgtg atccatgccg ttgcgttcaa cacattttga
     1861 actaatgttt cagttcggtt gtcgattttc ttttttagtt cttgcgtctg cgtgtttact
     1921 gtcgaggtat aaagagataa gtcattaccc aaaacaaaat ctttgtattt tccgagtgtc
     1981 gggaaataag tgtattcgac catgcgctct tttatctcga tatctagttc tttcgccctc
     2041 acatgagcga catcaccaaa atgtaaagct gctaactctt gatacatgtc accgtattcg
     2101 agcgtatgct ctagcgcgac catactaact gtgtgagttg cttttggttc gtgaatacga
     2161 tcattatcaa ataaggtttt accccattta atcaattcat caactgtttt gcagtcgcca
     2221 ttttctcgtt ttccaatgcg tctgttttcg ttagtcacac catcagtttc taaatatcca
     2281 tattcaatag gctctttgtc ttcgtcataa tcgttatcag gaactccgcc aatcaaatac
     2341 agactattga caattgattc ttcatcaatt tcttcttcga ttgcttctaa attgatgcca
     2401 aaatcaatcc gaaaaccatt atctgatcca atctgtttta ccagtttcaa atcatagtta
     2461 tccatatcaa gttccgcgct agtcacgcca gtcaaatttt gattaccgtt gtttgaacca
     2521 atgattgctt caattggcgc gacttgtctt gctgtaaatt ggtgcgttgt ccctacattc
     2581 gaaagatagt taaatttttg atcaaatgcc aaactgtttt taagattggt catgatctga
     2641 gacccattgc cgttatccgt aaacgacttg acaataaagt tcttattggc cataaaacca
     2701 atatgccgag ctgttacaga aacggaggtc aaattctttt tgacattata aatttcaaaa
     2761 tattgccacg aaccatccgg caccttcgct tttatgaaat ttcctttttt taagtaagac
     2821 cgatactgtc cgcttcttga atagttacca taaaaacgat attggccatt caacacacga
     2881 ttgatttctg gcaaatcttc ccaatcaact aacgacgctc cgttaacact caaatcatct
     2941 ggcatttttt tataagcata aataaattct tgcgtcacag atacacactc ctattccaga
     3001 cttttatttc tttgtatttt ccagatatga ttaattgatt tttaccagga ttaagcgttg
     3061 gccaatctcc acgagtaaac aatggggcgt cttcttgaat tgcctttcct agctcagtat
     3121 ctatcttcac gaccccggct agtgtattct ctacaagcaa agtttttgta ccaatcgtta
     3181 gcgacaaatg accgccttcg gacacaattt caaaataggg ttttgctact tcgtctccag
     3241 gatcgaaaat atcttctctt gattctgtga taattatcgg ttgttcgttc aatttctttt
     3301 ttaatggttg gcaacgaaaa gtaacatcga atgtataaaa gaagccccac tcgttttcga
     3361 atgggacttc tttatccatg ctgcagatag catctagata cttgtctgga tcattatgag
     3421 tgattaattg gcttttacca gtaagccatc ttttgacttc cctcagtttg gaatgtggga
     3481 tagtgatccc ttctatttct aaatcaaaag gttcataatc atcaaacgtc tccgtcaatt
     3541 ctccgcttcg gccctgaacc gtgtaagttt cgtatcgctt gttcggcata atatctggaa
     3601 gctctacctc aatgatgcaa tccatatcaa ttactgcatt ccgatttttc caaataaaat
     3661 ttggttcatc tggattcata aattgtcgac tcaaatagga acacctccca aatcataaat
     3721 agcctgttgg tttgctttag caaatttacg attcatacga tctaactcag atggattgtt
     3781 tgcatcgact ttgccaatgt gtatatgttg ttcaatgcta cctccggaaa ctcttccgct
     3841 gattcctttg cgtttttctt cgtctgataa tggagtaact gtggtttttc cattcttagc
     3901 ggtcagtaac tctggaccag cttcaccaac gattgcttgc ccgttgatca gatgaccacc
     3961 ttgtgctaga taagggattt ttgatataga gaatgatttc cctcctatac caggaaccca
     4021 ttttggtatc ttgatattat tcaatccacc aataaatcca ttaatcaatc caatcatggc
     4081 attaatagga gctttcccca cagcaacaat accatcgaaa atacctccaa aaatatcaac
     4141 tagaccttgc catgcttttg accaatttcc tgtaaatact ccagtaataa aatctagaaa
     4201 accactgaaa attcgttttc cagcattata aaaattattg aaatttgcta taacgccatc
     4261 gaaaaatcca ccaaaataac cacctaaaaa attgaatatt tcaacagcta catctgaaac
     4321 acctttgaag aaagaattca ctccatcatg aaaccattta acgttattat atgcccaaat
     4381 tagtccagta actaatgcgc caatagctat aactgctaat gcaaaccagc ccccagatat
     4441 tccaaataaa ccagcaagtc cttgccaaac tcctataaag cttttaactc ccccaacgat
     4501 ttttgtaaca gatcccataa gtgaaccaag aacgattagt actggtccaa ctgctgccgc
     4561 tatcccagca attgttatga tccaattctt tgtatcttga tctagagacc cccaccattt
     4621 agagaattcc tgtaataagg aagttgcttt ttcaaaaaaa ggcaataagc tgatttggac
     4681 ggattcgccg acgtctgcta aagcaaactt cgcattattc atcgcacgat tagcagcgtc
     4741 aataggatct tccgtttctg caaatgtatc agccactgta ccgccgcttt ctttggctac
     4801 ttttgctaaa tcatccaagg tgaacgttcc tcgtttgatt gcgtcaacca ttttatctga
     4861 cccttttcca aatacttcat tagcaattct cagcgcttct gtttcatctt ttgcattcag
     4921 tattgaatcg attgttccct ttaatccgtc ttgtaacgat agattgtctt tggcataaac
     4981 aactgaagct ttagacatct tgcttaacat tgtgcttgaa tcaaccccag cttgctcaaa
     5041 tttaccaatt aaggtcaccc cttcatcgaa actcaatcct aattgtttga tttgaggtgc
     5101 accatcaatc gccttcttca taaggtcatc aactgattgt cctgtgttct gggaagtttt
     5161 ggtagtaaca tctaatacat cgtttaaatc atcgtaggat agttcataag cttcgatcga
     5221 ttgtctagca taaattgctg cttgagagac atcagtatta ttgatatcag catatttcaa
     5281 taggtaatct gtcgaatctt ccagtttctt atccatgaac ccaaattgag tattgacttc
     5341 accaatcgct tctccgactg tttgtagttc taagtgagta tttgaaccaa cgttttcaaa
     5401 tgaggtagat aaacgatcag caacatcgcc tgttgcgcct gtcttttgta taatagtgtc
     5461 catagcttca tcaacttctg agaatgcggc taaaccagct gcacctgctg ctacaatagg
     5521 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     5581 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     5641 gttcgtctgt tctaatgctg tacgaagctt attttctcca gtttccgaat taagcaattg
     5701 cttatataat ttttctgatt cagcagaata ttcgcctgtc tctttgacag atttttcata
     5761 ttcttctctc aacatttgag atcgttgttc agctaacccc agttgttttt ctaacttttg
     5821 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     5881 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     5941 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     6001 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     6061 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     6121 cttcaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     6181 ccaaggagtt cctatctctt ccgattcttt ttgtttacct ttctttttgc ttgcgttttt
     6241 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     6301 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     6361 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     6421 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     6481 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     6541 ataaattcca ttgtttgcat agtaaatgag atagggaatt ctacaccttg aatagtgaca
     6601 gtagcagaat tttttaagtc ttcaacgtta attccataat cagataaccg agccattagc
     6661 ctgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     6721 tgaattttcc aaatagatcc attgtcgctt cttcacgatt cgaatcaaaa cttgcataca
     6781 tcacattatt gtatttcagt cctgtagaaa caaagtttgc agttacatca tcgatttttg
     6841 tttcatcttc agcagtagcg tattcttcat caattacatt agatagttgc gtgtttggat
     6901 accatacagc ttttttacct ccgccttcaa tatttccaat gaatccaaat gcaaggtaag
     6961 gaaattctcg tgcggtattt ttactaaatg ttactccgct ttttgcaatc atccctttga
     7021 tttcatccat taccgcaatt ggaataccta catgatccaa cgcaatttca tgttttgttt
     7081 ctcgggacac gcgtcggaac attttgcttg atgcccattt ttccaatgct gtaccattgc
     7141 ctttgactcc aatttttgta gcgataggca accggacaac ttcactataa gtcggagcag
     7201 ttccaacaga atctggcgtt gccatcatcg cgattaaaat gtcgtccaat ccttcaaaat
     7261 aaaaagtatc ttgctttccc aattaaaatc atccttccca taaatcaagt attttcttcg
     7321 tcatgatctc ttcgattttt tctttatttt gttcatacgt accgcttgca aagtgttgtg
     7381 ctttttgttt tgtcgtacca ttttcggtaa agcgccagta aaaagcagtc tcttcaaact
     7441 ggactttcac tcggtcttct tcaacaacaa cttttatttg ttcgctcata tgcttctttt
     7501 taagcaggga cttaggaata ttaggaagca actgctctag gtaaaacttt gcagcttctt
     7561 ctaaagattc caatgataat ttttttggat caactctaga aagattccct aaataatccg
     7621 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     7681 tagttggtca cggtatcgtc attctcatca ccttgtatac ctgtaaagtc tgaatatgga
     7741 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     7801 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     7861 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     7921 ctgaacactg gtaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     7981 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     8041 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     8101 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     8161 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     8221 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     8281 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     8341 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     8401 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     8461 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     8521 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     8581 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     8641 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     8701 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     8761 ggtgtgtctg gtttaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     8821 aattggtaac ctattccacc ttctgcttgt gtaaatggac gcaacaaagg aaatccatca
     8881 tctgttttca ttttttcaat agcagtcaaa gcagctcgat taataataaa gcgtcccttt
     8941 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     9001 ccagcacctg cagcagttag atctaaaggt ttttcaaatg ctacagcctt tttggctaat
     9061 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     9121 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     9181 agtaattttt tagttactgt tgccaaagca tcgaattcgg caggatcaag caaaatttca
     9241 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     9301 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     9361 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     9421 gggacagtaa ctgagccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     9481 atatttccaa ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttattg
     9541 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     9601 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     9661 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     9721 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     9781 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     9841 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     9901 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     9961 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
    10021 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
    10081 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
    10141 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
    10201 accgttgtgt ctggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
    10261 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtggttaaa taagcaacgt
    10321 acatcggagt tactaatcgt tctagcaaaa gcgcctggtg caatgacttc actcaaatcg
    10381 tcccataata gtgttgggct attgaataca gcagcatatc cactaatggt cctcgtatga
    10441 gtttcttcat cagaacgcgt tgaaaggttg gtgatgtcaa tcgtgcgaat ttccttcttc
    10501 ttcatttcca tcacctcctt tcaagttttg atcattcgtt ggtaaggaat catctgttgc
    10561 atttttctgg ccaatcatag acaagtcatt tgaaatatag atagcttgtg tttctggagt
    10621 gttctgttta ggaaaaccaa gcatttctgc cacattatct ggacttgtaa tcccagtacg
    10681 aacaatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatgttaat
    10741 tttccattcc actcgatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
    10801 cttgttcttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcgat
    10861 gtctgttttc atcaacgatt gatacgtatc aacattgatt cctaaaaatt tacctaagtc
    10921 ttttttataa acacctaaat agttcaaaat agccgcgtca tcaacaggac tttttaacgt
    10981 ctcgatggaa tatccttttc ccagaggaat catcttaaca gaatgatcac tgtcattttg
    11041 cgttccttcc agttgatcca atatagcttt gacaattttc gtttgggcgc tattatttgg
    11101 attgatgtgg gcgtccagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttatc
    11161 cgtcaaaact ttttcagcgc tcagaacgcc ttccagagtg tttcttgcaa gatcaattat
    11221 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    11281 tattttttgt ccattcattt caaattcttc ttgaagtcgt tcattgattt tagtggttac
    11341 accgtatgcc aaatgaagct ggtcccgatc tgttaatggg aatgtctcac cattgatcag
    11401 tagggtattt gtttccaatt tggtaaattc gaatccggtc aaataatcat tgggattctt
    11461 caaaattttt agcaagtggt ggtccttcac ttcattaccg tctggaccta tgacaacagg
    11521 tgaggacaac gctacctggt ttgagatatc ctggaccaat tcataaacat cagaagattc
    11581 catgatagag gaatctgtta catatctttg accgtaacgc gtatagtggc caaacatatc
    11641 ctcgatgtac ccacgctttt ccataaagga atagactgca ttcgataacc gatcacgtaa
    11701 tttcaatatt tctcaccgcc tttctattta tctatagatg gaactaaggt aatcatctaa
    11761 ttcatccgaa tcaatatctg tcatctgatt catcgtttcc ttatgaccac acaaaaacgc
    11821 cacgaaacca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccgttgat
    11881 gttagtgatt gctacaacgt tcaaggtgca ataaaggaac aatggattat caaattgaat
    11941 tcgtttctca taaaacaacc gttcgacatc atcaaaagga gcgttcaaca ctttaggata
    12001 ttgggcaacc tcaacgcatt ccaatcctaa gttctccaat ttctcaacaa gtttgtcgct
    12061 catcgctgga tcataattca cttgttgtat atcgtataaa tccatgcagt cttcgatgta
    12121 ctgcaagatt tgatcttgat caatcatttt accatcgcaa aattcaacaa aaccttgttc
    12181 agctaaatcg ctgtaaggca cgttatcttc tttttctcga aactctaaat cttcattggg
    12241 aataaaataa agctgcttca ctttaaggac cgcttttcca tcttcatccc acgttgggaa
    12301 gtttaaagac acacaggtca aatctcgtgt gcgtgataaa tccaaaccaa tgtaacaagg
    12361 ttcaccactt aaatttccaa gctcttgggt agtaaccaaa cacggctcta cttgatcctg
    12421 ttcaaagaaa ttatccgcac cgtttacaaa tacatccaag tgcttcgtta gaaactcggc
    12481 ttttgaatgt gctgaacgtt gtgccgtttt aaacgctgac tccaaggcgg aaagatcaac
    12541 tgatatcccc cagttaggat tgcacatttc ccaaactttt ctatcagtcc aatcatatcc
    12601 tttatttggc tcatagatta aaacaaacga tgagtcattg tcatcacgtt ttaacacttc
    12661 tttcgcttca cgataaacac gcatgccaac cgagctgcta cctttacccg ctgttgaaat
    12721 attaaacatt aatggttgtg gtaatgatat ctgtgcagac ttaaaattat cgtattgttc
    12781 cattttttcc tgtttgtgca actcatcatt acagacaaaa tatggattcg acccctcaat
    12841 attttcaata tttttcgttt gaacaataaa tttgtttttc agtgctaaat ctccatctaa
    12901 gtattcataa gtaatacttg atacagtacc ttttgggcct ttaaatattt tggtgccatc
    12961 aagcaaaaca ggattattta aaatggagtc agcaaacggt tgtgcagcat attgcgcctg
    13021 agcaaaatca gaagcacatg cataacagtt aacagataaa gcaccttcac catacatcgc
    13081 atatcctaaa gcacccactg caataagtgt tttaccattc tttttgggaa tttggacata
    13141 tgcctcacga gttacacgga caatctgacc tttttcgttt tctcgtaccc aaccataaat
    13201 ccatgaataa ataaactttt cccaaggctc cagaataaac ggttttccaa ccatgtcacc
    13261 ttttgtatga acgataaacg actcaaccca gtccatcatt tcattcgcac gatctacatc
    13321 aaaccaaata tctttacgtt ttttccaccg ataccaacga tccactgcca aacgaacagt
    13381 tttaggatat ttcccaggtt tctttcttac ttcttttgca aataaatcgg cataatttac
    13441 accaggttcg atcatttttc agtacctgcc ttcttacgcc atttgtttct gtgcgctgct
    13501 agttcatcta ctggcttttc ttctggacgt gtaatttctt catcttttct tgcagtcgat
    13561 ccgccagtta tttgtctacc agttttagcc ttattcgtta gccccaacaa atctagagct
    13621 ttcattttct tatctgacca agtttctact tgctgcgcca atggatgctt cgattgatta
    13681 gtggcacctg atttattcgt gaatttttgc gtctccggaa agcctttttc cttccacaaa
    13741 agatatttgt gttggtaaat ttcaaaaata tccaaatatg attcgattaa tggatcaaga
    13801 gtgatggtgt acaaatcaga caaattcatt atttttaaaa tacgagcttt ttcagcactt
    13861 actttttcat caacaatcgc tttacgttgc gctttagtcg tcatacttgt atacaccccc
    13921 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    13981 cacgcgaaaa aattttgaaa atggataggg gggcttcaat taaaatacga agggaaaact
    14041 tttttgtctt ccgtttcatt ttcaataatt ggatgacatt ttgaacataa aagcatgaga
    14101 ttgtttggat caagcttaag cagttcattg tctttgatcg gtacaatgtg atggacgtgt
    14161 gccctcttgc caaagatgaa ctgaccgcat cgctgacagt gaccaccttc tctttcataa
    14221 ataaattgac gcatatcttt ccatgcctgt gttcgataga atggtttgtt ctcatgatga
    14281 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    14341 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattctg
    14401 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    14461 catgtttctt agcatatgca cgcaattctt ttgcagtcat gttgtccagt tcatccgtat
    14521 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa atcagcagca gtgtttccat
    14581 cgctatcaag aatttcactg ctattagtaa taagttcacc attaacagcc acaaacgttt
    14641 taccatcact taatattcct ttttcttccg ttgcttcaaa atcaggttct tgacctttcg
    14701 gaactacgac agtctttttc ttttctgaat cccaatactc tgttcctgtg atggttgttc
    14761 taattttgat cattgccatt ttgattctct cctttgtaat ttgtgctaat tacttttgca
    14821 cccattcgtt cataccattc aacagtttcc tgcaaattgg gtaaagtgtg ggataataaa
    14881 gaaataacca acacatccat tgctatccca cgattatctt tatctacttg cacatttgca
    14941 taggtgccgt tccaattgtt tcgtaaacca gtaggactgt tctctgcttc tattgctttc
    15001 ttatagacat ctcccatatt cgttgggaca ttcgccataa gcattgcctc atgaaaatca
    15061 ttcatagata aaactccttt caaaataaaa agaccactca aagagtgatc taatatgtaa
    15121 tagcaaccta cacacagaca agtctaatac ttcctgcgcc tcaccactgc cacatccctc
    15181 ggttgctaac gaatactgaa acagaatacc gtctctgttc atgaccaacg cgacagtaca
    15241 aagctttgtc gcaagcaggt tatcgtattc ctaataaccc caactaatgt ttctctccta
    15301 acctacacct gagagtggcg cacgtctgcg ctagtatttt atgccttcta actgctagta
    15361 gcatcccgac tagtcagcca attcatcggc atccgataag atgaattgtt tccgtaggtt
    15421 ccttaagtca ctggcaatga atcgaacctt gcatgagtac aataattttt gataagggac
    15481 ttttgatatc agatatacgt ttccgatacg catgtactct ctccctcaat gcgtctaccc
    15541 tttccgccac aatgacataa tgacaataga cagcaacgga tgatagataa taagaacaat
    15601 ttagaaggag ttggaattca catccttatt cttaatattt ccgtcgctat ctatcgaagc
    15661 ttaattgtga aacaataata aaacgatgtt ccttttatta ttattttgtc tcagacctat
    15721 cactaatctt tcgacactat cataatacaa cattgaatag gtaagtgatt ggtataaaaa
    15781 aggtataaaa tggaaaccaa attggtaata aaagggtata aaaagtgtaa aaactggcta
    15841 cttgaaagca accagttcta acgatgaagc aaattggatg ataattctgt ttgattctac
    15901 tttaaccgat tcttcgctag tattattcct ttgagcagtt acataaatgg gcagaccatt
    15961 gatataacga tcatagaata tcttcttgcg cctttcagtc acatcgggtt tgtgcggatg
    16021 ctgtatagca gagtatcctc taataaacaa tttatgcaaa tactcaaatt cttcttgtgc
    16081 ttcttcttta tcaatcaaca ttctttcggc ttcaaaatca tgattagctg tagatggtgg
    16141 aaccaaggag tatgatgctg tcacttttgg ctcacgaggc tgccctactc tacatctagc
    16201 tgatagatat gctgaaagaa acacagcgac attatgtttt gtgcgttcca tatcaacatc
    16261 tttcgcgctt ggtgtctcat atttctttac atcgaaaagt accatcctct gattccccca
    16321 attatgatat aatacttatg tcggaaatat tattcatagt cggagcgatc cggctttttt
    16381 attttgtacg tgaaatgagt tctcctgatt tatacgcctc agcaaattca accaaagcta
    16441 ctgccttcat tctctcgata gttctatcgg aatatcctaa tttttctcct atttgataca
    16501 tcgacatctt attaggaaaa caataactat aataaagtac ctggaaatgg attctactta
    16561 gccgtgttaa tgcttcgata accgaatctc gagtacttat cgcttcaatg atttcttcgt
    16621 tttgactgtc aattgtgaat ttcaaatttt tggtaacagt catcacggaa aagtctattt
    16681 tcactggtcc gaccaaacgt tctaaatttc tgtatctttt tagtaatcgt ctagcatttt
    16741 tccttgtttg cttaatatct atattctcta aaatgttcac aattgcctct tctccttcca
    16801 gttgtggaat aacgctcatt gttgtaggat agttgtaaaa aagcaaaatt aagattttat
    16861 aacttccata tccaccaatc tcgctacagc taaattctct ttgcttttcg ctaaccactt
    16921 gtcacattcc atcgtgtttt caatgcggat gattgctgag tgattataga gatgctctac
    16981 atatccacga aacggataga tgaactcctc tgcttcacag cgaaccatgt caccgacttt
    17041 gacttttggt ttcttacgtg ttttagggtt ctttgtcggc atatctagca ttaaaccgcc
    17101 gataccatga ctactagcgt aaaatccgtc ttttagtttc attatttctc ctccaataac
    17161 tcgctattct cgtatatatt tccgatgact tcatacgtgt attcttcaaa cagctctgtg
    17221 ttaaaaattc gatactctaa atcttcatca acagtggcac aaactaatcc cgaatgtcct
    17281 attgaatttt tgacaataca aactttatta tctaagtaat cgaatccatt tcgcacgctg
    17341 actgatacta catccccttc aaatat
//
LOCUS       DACEOM010000066        17272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEOM010000066 DACEOM010000000
VERSION     DACEOM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.067"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(176..1462)
                     /locus_tag="IW781_002210"
     CDS             complement(176..1462)
                     /locus_tag="IW781_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1183201.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IW781_002211"
     CDS             complement(1455..2723)
                     /locus_tag="IW781_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1183202.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IW781_002212"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IW781_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1183203.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IW781_002213"
     CDS             complement(3495..3941)
                     /locus_tag="IW781_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183204.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="IW781_002214"
     CDS             complement(4034..4279)
                     /locus_tag="IW781_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183205.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="IW781_002215"
     CDS             complement(4394..4972)
                     /locus_tag="IW781_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1183206.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="IW781_002216"
     CDS             complement(5049..5579)
                     /locus_tag="IW781_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1183207.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IW781_002217"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IW781_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1183208.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="IW781_002218"
     CDS             complement(6436..6831)
                     /locus_tag="IW781_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1183209.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /locus_tag="IW781_002219"
     CDS             complement(6828..8240)
                     /locus_tag="IW781_002219"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1183210.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IW781_002220"
     CDS             complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IW781_002220"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1183211.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9095..10555)
                     /locus_tag="IW781_002221"
     CDS             complement(9095..10555)
                     /locus_tag="IW781_002221"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1183212.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="IW781_002222"
     CDS             complement(10725..11882)
                     /locus_tag="IW781_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1183213.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IW781_002223"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IW781_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1183214.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="IW781_002224"
     CDS             complement(13349..13876)
                     /locus_tag="IW781_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1183215.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="IW781_002225"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="IW781_002225"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1183216.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="IW781_002226"
     CDS             14909..16156
                     /locus_tag="IW781_002226"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1183217.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="IW781_002227"
     CDS             16232..16576
                     /locus_tag="IW781_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183218.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="IW781_002228"
     CDS             complement(16693..17019)
                     /locus_tag="IW781_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183219.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT         4701 a         3704 c         3157 g         5710 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac gg
//
LOCUS       DACEOM010000067        17178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEOM010000067 DACEOM010000000
VERSION     DACEOM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.068"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW781_002229"
     CDS             complement(543..2048)
                     /locus_tag="IW781_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1183220.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW781_002230"
     CDS             complement(2045..2731)
                     /locus_tag="IW781_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1183221.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW781_002231"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW781_002231"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1183222.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IW781_002232"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IW781_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1183223.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IW781_002233"
     CDS             complement(4752..5321)
                     /locus_tag="IW781_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1183224.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IW781_002234"
     CDS             5507..6409
                     /locus_tag="IW781_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1183225.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IW781_002235"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IW781_002235"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1183226.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IW781_002236"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IW781_002236"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1183227.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IW781_002237"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IW781_002237"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1183228.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IW781_002238"
     CDS             12977..13162
                     /locus_tag="IW781_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1183229.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IW781_002239"
     CDS             complement(13224..14420)
                     /locus_tag="IW781_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1183230.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IW781_002240"
     CDS             complement(14590..16026)
                     /locus_tag="IW781_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1183231.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IW781_002241"
     CDS             16319..17128
                     /locus_tag="IW781_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1183232.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5042 a         3594 c         2983 g         5559 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctgaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaac
//
LOCUS       DACEOM010000068        17008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEOM010000068 DACEOM010000000
VERSION     DACEOM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.069"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..127)
                     /locus_tag="IW781_002242"
     CDS             complement(<1..127)
                     /locus_tag="IW781_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ1183233.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="IW781_002243"
     CDS             complement(231..542)
                     /locus_tag="IW781_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1183234.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="IW781_002244"
     CDS             complement(544..741)
                     /locus_tag="IW781_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1183235.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="IW781_002245"
     CDS             complement(1135..2862)
                     /locus_tag="IW781_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1183236.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="IW781_002246"
     CDS             complement(2855..4048)
                     /locus_tag="IW781_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1183237.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="IW781_002247"
     CDS             complement(4235..4879)
                     /locus_tag="IW781_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1183238.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="IW781_002248"
     CDS             complement(4973..5107)
                     /locus_tag="IW781_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1183239.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IW781_002249"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IW781_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1183240.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="IW781_002250"
     CDS             complement(7238..7711)
                     /locus_tag="IW781_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1183241.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="IW781_002251"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="IW781_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1183242.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="IW781_002252"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="IW781_002252"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1183243.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="IW781_002253"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="IW781_002253"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1183244.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="IW781_002254"
     CDS             complement(11758..12897)
                     /locus_tag="IW781_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1183245.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="IW781_002255"
     CDS             complement(13206..13919)
                     /locus_tag="IW781_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1183246.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IW781_002256"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IW781_002256"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1183247.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="IW781_002257"
     CDS             15108..16640
                     /locus_tag="IW781_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1183248.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17008)
                     /locus_tag="IW781_002258"
     CDS             complement(16831..>17008)
                     /locus_tag="IW781_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1183249.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3495 c         2910 g         5324 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaataca
//
LOCUS       DACEOM010000069        16756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEOM010000069 DACEOM010000000
VERSION     DACEOM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.071"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..594
                     /locus_tag="IW781_002259"
     CDS             133..594
                     /locus_tag="IW781_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183250.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            607..1467
                     /locus_tag="IW781_002260"
     CDS             607..1467
                     /locus_tag="IW781_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183251.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            1505..1894
                     /locus_tag="IW781_002261"
     CDS             1505..1894
                     /locus_tag="IW781_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1183252.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1881..4328
                     /locus_tag="IW781_002262"
     CDS             1881..4328
                     /locus_tag="IW781_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1183253.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            4333..6441
                     /locus_tag="IW781_002263"
     CDS             4333..6441
                     /locus_tag="IW781_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1183254.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            6438..7442
                     /locus_tag="IW781_002264"
     CDS             6438..7442
                     /locus_tag="IW781_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1183255.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            7461..8372
                     /locus_tag="IW781_002265"
     CDS             7461..8372
                     /locus_tag="IW781_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1183256.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            8559..8846
                     /locus_tag="IW781_002266"
                     /pseudo
     CDS             8559..8846
                     /locus_tag="IW781_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9034..9249)
                     /locus_tag="IW781_002267"
     CDS             complement(9034..9249)
                     /locus_tag="IW781_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183257.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(9508..9675)
                     /locus_tag="IW781_002268"
     CDS             complement(9508..9675)
                     /locus_tag="IW781_002268"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1183258.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(10020..11459)
                     /locus_tag="IW781_002269"
                     /pseudo
     CDS             complement(10020..11459)
                     /locus_tag="IW781_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(12007..12678)
                     /locus_tag="IW781_002270"
     CDS             complement(12007..12678)
                     /locus_tag="IW781_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1183259.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(13027..14478)
                     /locus_tag="IW781_002271"
     CDS             complement(13027..14478)
                     /locus_tag="IW781_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1183260.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(14590..15933)
                     /locus_tag="IW781_002272"
     CDS             complement(14590..15933)
                     /locus_tag="IW781_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1183261.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(16067..>16756)
                     /locus_tag="IW781_002273"
     CDS             complement(16067..>16756)
                     /locus_tag="IW781_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1183262.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT         5326 a         2989 c         3049 g         5392 t
ORIGIN      
        1 catggatagg agatgggagt acttttgaga atttaggata tcaatatagc gacagaaata
       61 cttttcttaa tggagtcaat aagtttgaag aaagattctt ttctaaatat tgcaaagtat
      121 gtggggagtt agatgattaa attttcgtgt gaccgtattg tcattgaagt aagggatgaa
      181 gttcttaaga tatttgaaca atatcggcag ggaaaggatg acttgcctga agctggagga
      241 gttcttcatg gaaaaagact taagaatggg agcataataa ttaccgatat aacaacacct
      301 caaataggag atatacaaaa aagattactc tttaaaaaaa accgagaaat tcatcaacag
      361 ctttctaatg agaaatggat agaatcaaat aagttttcta ttgcattggg agaatggcat
      421 actcatcctg aagctgtacc aactccatca aatgttgata agaaaagctg gagactgaac
      481 gtgagcaagc aacatgatga tagagtttat gtttttatta tcgttggatt ttctgatttg
      541 agaatgtggt tactgtcaaa aaaatcagat attaggcagg ctataaaact ataaaggagt
      601 gattaggtga aatcacaaaa gaatatttgg tggtgggggt ttttagtagg aaaggtaatg
      661 ataccagccc ttctcttaat ttctctaccg atgattactt ttttctttac caataaaaaa
      721 atgagtaaag aagccgtgat ttttttcttt ggagaccaaa aagctacctt tattgaaacg
      781 ttagtaacat ctcttcagct taatttgaat tacatttttt caattattat tgttatatcg
      841 ttattgaatt tttttaaaaa aagaaattca ggaaaggtgt ttaactctaa tggaaatgtg
      901 tactacaact atttttattt tgtattttgg gtagcagcaa ctttacttgg ttatgataaa
      961 attcagattg caggaattcc tatacacatg caatataagt tggttttgag tggaattttt
     1021 tctgaggtgt tgccagatat atacgatgat cattacgaca gtgatggaac atgtaaagta
     1081 agcattgaaa aagaaaattt cgatgacatc gatggatatg actctgtgaa cttactgatt
     1141 attgatactt acgatataaa aatgagtgaa ttatctatgg aaaatcaaac atatcctacg
     1201 ataattgtta gagggaattc aatagatgga gttcgtaaag ttaatagatc tttaatcttg
     1261 gaaataaaaa aaacaatgga tgaaattcaa aaaagtgact ttaaaaaagt atttgttgct
     1321 tcaacatcta atccaaaaaa tagtatcaat attattaatt catcgtttag atttttcggt
     1381 cgatcacgta ggtttaaact ttatgttctt caaaaggatt atgcaagcaa tggaaagtat
     1441 agtaagaaat atagaatttt tatttaagaa ggaatttttc cttcttttta ttttggagga
     1501 aactatgaaa aaaataaaaa gctacaccag tatttgggca gttgaaaaag tcatttatgc
     1561 gattaatgac tttcagctgc cttttcctgt gacctttaac cagatggctt ggtttgtttt
     1621 atccttgatg tttgtcattg tatttgccca tgtgcctccg ctatccatga tagagggggc
     1681 ttttttaaaa tacttgggca ttccgatagc ggtcacttgg ttcatgtcgc aaaagacctt
     1741 tgacggtaag aaaccattgg gatttttacg atcctttatc agctatcact tacggtttaa
     1801 ggtcactttt gcaggaaaga aagttaaaga acagaagaaa cgatgggacg aaccaatcac
     1861 cttggtaagg agtgtgaatt atgtacccga ttaagtatat tgaaaataat cttgtcttta
     1921 accaagaagg cgagtgtttt gcttactatg aattagtgcc ttacaattac tcctttttgt
     1981 cacctgaaca gaagtatcaa gtacatgata actttcgcca attaattgcg caaaatcgtg
     2041 aaggtaagat ccatgcctta caaattgcca cagaaagtag tattcgtgcc acacaagaac
     2101 gttcgaaaaa ggaaattacc ggtagactca aagaagtagc aaagcaacga attgatttac
     2161 agacagatgc attagtatcc atgattggcg atagtcagat tgattatcgc ttctttattg
     2221 gctttaaact gatttcgaca gatgaagagg tcaatctaaa gaatctcagg aaatctttct
     2281 tttctggact tcaagaattt gtctatggag tgaatcatca tttgatgggt gattttgttt
     2341 ccttatccaa tgaagaaatt cgtcgctata ccaagcttga aaaactaatg gaaagtaaat
     2401 tggctcgaag atttaaagtc aaacgagtaa cgccgagtga cttgacgtat ttaattgaac
     2461 atatctatgg tgaaaaaggc acaccgtttg aagaatatga attccagcta ccaaagaaaa
     2521 agctgaaatc agaaacgctg gtgaaacgtt acgacttatt acgtcccact cgttgtttga
     2581 ttgaagaaaa gcctcggtac ttacggatgg aacatgaaaa tcatgagtct tatgtagcgt
     2641 acttaacaat taataccatt gtaggggaaa tggagtttcc ttcctcggaa cttttctatt
     2701 accagcaaca acaatttacg tttccgattg atacttcaat gaatgtggaa attgtgacga
     2761 ataaaaaggc cctagctacc gttcgtaata agaaaaagga attaaaagat ttagacaacc
     2821 atgcctatca atctgataat gaaacgaact ccaatgtatt agatgctttg gattccgtgg
     2881 atgaattgga aaccaccttg gatcagtcga aagaatccat gtataaacta agctatgtgg
     2941 ttcgagtgag tgctaaatca gtggatgaat taaaacgccg ctgtgatgaa gtgctcgatt
     3001 tttatgacga taccaatgta aaactagtcc ggccctttgg cgatatgatg ggtctacaca
     3061 aagaattctt accctcaagt aagcgataca tgaatgacta tattcaatat gttacgtctg
     3121 actttttggc gggacttggt tttggggcta ctcaaatgct aggagaatta gaaggcattt
     3181 actttggcta taacgtagat acggggcgca atgtttacct gaaaccggca ttggcatcac
     3241 aaggggtcaa aggctcagta accaatgctt tggcatctgc tttccttggt tcgttaggtg
     3301 gggggaaatc ttttagcaac aatctcttag tctattatgc cgtccttttt ggtgggcaag
     3361 ctgtgattgt cgatccaaaa ggagaaagag gcggttggaa agaaacgttg ccagaaattg
     3421 cggatgaaat taatattctt aacttgacca gtaagccaga aaatcaaggc ttacttgatc
     3481 cctatgtgat tatgaagaaa aagaaagact cagaaagttt ggcgattgat atcctgacct
     3541 ttttaacagg gatttccagt cgggatgggg agaagttccc tgtgttacgc aaagccattc
     3601 gaacggttac ccaaagtgat cagcgaggat tattacaagt aatcgttgaa ttgcgtaaag
     3661 aggactcacc agtcgcggaa aatatcgcgg atcacatcga gtcctttacc gattatgatt
     3721 ttgcccattt gcttttctca gatggttcga ttacccagtc gattagttta gataagcagt
     3781 taaatatcat tcaagtagcg gatttggttt taccagatgc agaaaccact tttgaagaat
     3841 ataccaccat ggaattgctg agtgtttcca tgctaattgt tatttccact tttgccttgg
     3901 actttatcca tagcaaccga gaaattttca agattgtgga cctggatgag gcttggagtt
     3961 ttcttcaagt agctcaagga aaaacgttat cgaataaatt agtgcgtgct ggtcgggcca
     4021 tgaatgccgg tgtttacttc gtcacgcaaa acagtgatga cttgttggat gaacgtttga
     4081 agaataatat tgggctaaag tttgcatttc gcagtaccga tattcatgaa atcaagaaaa
     4141 ccttggaatt ctttggatta gataaagaag atgaaagtaa tcaaaaatgc atgcgagatt
     4201 tggaaaatgg gcaatgcttg atgcaggatt tgtatggtcg agtaggtgtg gtccaagttc
     4261 atccagtctt tgaagaactt ttccatgctt ttgatacccg tccgccagtt caagagagaa
     4321 tggagtgagg agatgaaaaa gaaacttttt cgtatctttg gaattgtttt aatcagtttg
     4381 gtcactctga taatactttt gagcttagtt ggaacggtag ctgaggcaac aggtctggtt
     4441 gatgatacag ttggagccgg aaatctttat tcccaatatt cacttggtaa ctatcagttg
     4501 gatttctttg tggatagttc gtgggattgg ctgccttgga attggggtga tggcctaggg
     4561 aagagcgtaa tgtatggact ttatgccatt actaatttta tttggacagt tagtctctac
     4621 ttatcaaatg ccacgggcta cgtggttcaa gaagcctata agctggattt catttcagat
     4681 acggctgaaa gtattgggaa gaatatccaa accttagccg gcattaccga aaatggactc
     4741 cagacctcgg gcttttactt tgggtttcta ttattgatga ttttagctct aggagtttat
     4801 gtggcgtata caggattatt aaaacgagaa actaccaaag cagtccgggc tgtcattaat
     4861 tttgtgatga tttttctact ttcgggttcg ttcattgcgt atgcgcccac ctatatcaca
     4921 aaaatcaatg attttagctc agatgtcagc gaagcggctc ttagccttgg aaccgagatt
     4981 gtcgtgccga attcagaaag ccaaggaaag gacagtgtgg atttaatccg agatagcttg
     5041 ttttccattc aagtccaaca accgtggtta ctcttgcaat ttgatgattc caatatagaa
     5101 gaagttggcg aagatcgtgt taacaaaatc ctgtctgtaa gtccagatga aaataaaggc
     5161 aaggatcggg aagaagcggt caaagcggag attgaagata atgacaatgc gaatctcagt
     5221 attaccaaga ccatgaaccg cttagggacc gtggtctttt tggtgttgtt caatattggc
     5281 atatccttct ttgtctttct cctaacaggg attatgttgt tctcgcaaat tctctttatt
     5341 atctttgcga tgtttttgcc gattagtttc ttattgtcca tgttgccgac ttatgagagt
     5401 ttgggcaaga aggcgattat tcgcttgttc aatacgatta tgatgcgagc aggagttact
     5461 ttggtgatta cgactgcatt tagcatctcc acgatgtttt tcaatatttc ggcaacctat
     5521 ccatttttca tggttgcttt tctgcaaatt gtgacctttg ccggtatcta tttcaaattg
     5581 ggcgacatta tgaatatgtt caatctccag agtaatgata gccaatcaat gggaagacgg
     5641 gtcatgcgaa aaccgcaaat gttaatgaat cgaacattgc ggcaactcaa tcgaaatgtg
     5701 ggacggactt tggcttttgg tggtggtgca gcagttggaa ataagttggc aaaggaacaa
     5761 tcaaaatcca aatttaagcc atccggttct tctctacgaa agaattcacg attaccgaac
     5821 gatcacgaga taccatctga cttgaataag gaaaagccaa ttccaaataa taagaaacaa
     5881 tcccgaatga atttggccgg gagaaaagtc ggcaaggttc tggataccca agctttggtg
     5941 aaagataaga ccaaacaagt aaaggatgga gtacggaata cgccaaccaa cttgaaatac
     6001 aacttccata aaggaattga aaaaacgaaa aaagcaccgg aagagtttaa gcgaggactg
     6061 gtccaagaaa aagcaaatcg aggagagttg cgagaaaaac aacaacaacg tagagacgaa
     6121 aaaatagctg aaaagcgcaa agtattgaac gaaataggga atcctcatgg gaaaagaagg
     6181 gaaaatgttc cgataaaagc aaaggtccaa tctcaaaagg atagagcacc aaagcggatt
     6241 gtgagagcaa attcgaatcc cgaaatcaaa cgaaaattcg ctagccaaga aatcatttca
     6301 aaaggaataa accaatcctc aagtaagaaa aattcttttc aacaagtgaa acggcggtct
     6361 actctgtcca aacgaaccaa tcgaaaagta caaaaaagtt tgaaccctca tcccaaacca
     6421 aggtcaggtg agaaaaaatg aacgtaaaga aattaagtgc catttcgtta actttcctaa
     6481 caatctcttt tatgggtatc ttgatgtgtg tgggcttatt gtttggtgaa gacagtgaag
     6541 gaagtagtag ttcaggtgct cctcctgggg ggagtagtgt ctcagaagag gttttgaaac
     6601 accgtgcaat ggtggaaaaa tatgccaaag aatcggatat aacagagtat gttccgattc
     6661 ttttagcgat tattcaagtg gaatctggtg gcacaatgga agatgtgatg caaagttctg
     6721 agtcagccgg tttaccaccg aattcattaa gtaccgaggc atccatcaaa caaggatgtc
     6781 tctattttgc gtctttggtt aagcgttcca aagaattagg ttgtgatcaa gatagtatca
     6841 ttcaggccta taactttggc ggtggttatt tggactatgt ggcaaaaaac gggaagaagc
     6901 atagttttgc tttagctgaa tctttttcaa aggaaaaatc ttgcggtcaa aaaatcgatt
     6961 accctaatcc catcgcgaat aaggaaaatg gtggatggcg ctataactat ggcaatatgt
     7021 tttattgctt attggtgaag caatacctaa ctacaacaca gtttgacgat aaaacagtcc
     7081 aagggatttt tgacgaggcg tttaagtatg aaggaacggc ttatgtattt ggtgggtcaa
     7141 gacccgaaac aggatttgat tgtagtggct tgactcaatg gtgttatgcc aaagttggtc
     7201 tgaaacttcc tcgggtggct caagatcagt atgatgccat gacccatatt gacctaaaag
     7261 atgccaaacc aggggatttg gttttctttc attcgactta tgatgccggt acttatgtga
     7321 cccatgtcgg tatttatgca ggggagaatc gcatgtttca tgcaggagat ccagtaggct
     7381 ggacgaatct aacagaaagc tattggcagc agcatcttat cggtgctgga cggtacaaat
     7441 aagagaaaag aggaaaaaga atgaagataa aaattgaacg gaatcaaaag gaaaagagcc
     7501 taaaaaagaa aaaagaacga gtcgtatccg tgggtaagca tcgaaaaatg gtgcttgccc
     7561 tttggctttt attatcttgt agtcttactt ttggcgttta taaaaatttc accgccattg
     7621 atcaacatac aactcatgaa aaagtggtta ttaaagagaa agtagtgaat acttctggta
     7681 ttgaaaactt tacgaaggat ttttcgaaag aatatttttc gtggaaaaat aacaaagaag
     7741 caattgaaaa gagaatgaac aacctcgaac agtaccttac agaagaaggt ttcgctttaa
     7801 gtcaagatat ggttcgagca gatattccca ctaattccga agttcagtca gtaaaagtct
     7861 tagatgttga aaagagctcg gaggaatttg tcgtttcatt tttagtggag caaaagatta
     7921 cagagggaaa aaaggcacaa tcgatttctt ctgcataccg agtgacgatc tttgaagatg
     7981 aaaacaggaa ttatatagta acgagtttgc caactatgat tgcaaaacct gatagggcaa
     8041 aatacaagac aaaacaagta gaaaatgatt cgaaaattga tgccaaaact acagaagaaa
     8101 tcgctgagtt tttggaaacg ttctttaaat tatatccatc agcttctgaa aaagaattgg
     8161 agtattatgt tgaaaatgat gcgatgcgac caatcaatat tgacttaaag ttttcgaagt
     8221 tagaggatcc agtttaccta caaaacggaa aaaaaattct agtgagtaca aagataaaat
     8281 accttgataa tgtatcaaaa accactagct actttcagta caaattaata ttacaaaaaa
     8341 atggaaactg gaaaatcatt gatgttaaat aataatgact caatctaaat ttatgagaag
     8401 ttataagtag tttaacttat ttcaggcaga agtcgcccat cctctatagg tgggtgatga
     8461 atgccgttcg gtaactcgaa aaccgacaat taagacatag tgccttttta ttttaatcca
     8521 tgtcataatc agtctatata agaaatggtt gatataccat ggaattagat acaaataatc
     8581 attcattatt tcttctttgt taccacctta ttttagtagt gaaacattgt aaacatctca
     8641 ttgatgatgt aatgttagct tatgcaaaaa actatctttg aaagaatatc agaatcacat
     8701 cacattacct tagttgagtg aaatcgtgat aaagactact ttcatattat gctcaaagca
     8761 caacctaaaa ccgaattaac aaaattcagc aatgcctaag ctcatgtctg ataaaatgag
     8821 actttctaag ggtcaaaaat ttcctttgaa tacgatcatt agcttagata aaagtaaaaa
     8881 ccttagaact acggggatag cttggttaat aagagatatc gctgttaata aagacataag
     8941 cttgcaagta tgctctattc ccaagaatct cccacttcaa gcactaagga tcgttaaatt
     9001 cagctaagtg gcaagtagtt cactagacga taattatctc catatttcaa tctcagcttt
     9061 tccatttttc atagcaaatt ccgttatttt tatagcatct aaaagattca gcttttccac
     9121 tgatctttca ccacttttca gcttttttat tgtgcttaaa ttgataccag taccttttga
     9181 tattgaattg gaagtaaaat cattaagggt tttttttatt aaattcatat ccgcaattcc
     9241 agcgttcata agaatgaaga ttctcctctc atagactact ttgaaaatgg tactacagtg
     9301 gctgtttata tatttcagta ggttactgta gtggtcattt tattataatt agaaaaggaa
     9361 cctaaatcaa aaatgctaaa gaaaattttc atagtattaa acctatcatg actaaagcca
     9421 cggaattctt gataaaagag tatacttgca agcctatgtc tttactaaca gcgatatctc
     9481 ttattaacca atctatcccc gttgttccta aggttcttga tgttttctaa ggcaatgctt
     9541 gtatacatag cccttgatca aaatattgat actaacgttg acgtttcgat catgatgtgt
     9601 acggcaaata ggacaagtcc attctcgaat tgtgaatgac ttcttaccat ctgtgtgtcc
     9661 acattctgaa ccaatttgac tagacggaaa tcatttatct atcttgatga tttttttgac
     9721 agaagtatcg aaaatcttta cataatctgg gtgttgcatt cttatgcaga tgagagcgtt
     9781 agctccgtat tcataaccag ttgcctctaa aacgtagtcc atcggtggat acctagcttg
     9841 cggttgccag tggctttcgc agtcttcttg taatcaaatc gagcatctaa ttgagtacta
     9901 gtattttgaa gataatactt gagtttttcg gttttcagaa gttcacttca tcttccatca
     9961 tccatggaaa tcccactcac ccatacgcag gcctccaaaa attcttcaac aatggttttt
    10021 cagacatttt tctgaatacc tccttgtttt ttgatacgcc agatagtcag gaaaaaggca
    10081 acaaaacaca aaacaaagct gcctaaaaag gtaatcttca ttccatataa aaagacatcc
    10141 ggccgatctg cgatatagtt agttactcgt tggcccatct tagcagacat cgcttgatat
    10201 aagaccgtcg tagaaagcga aatcccgatg accatcccca aatttcgggc aaaggaattc
    10261 atggagcctg ccagtcccaa attttcgata gtgacagaac tcataaccat cgtattattt
    10321 ggggactgga acaaggcatt gccggcaccc atgatggcag tagcgacaat gtacagccac
    10381 aaaggagagt ccactgacaa aaacatatac atgacttggg ttaccgacaa taaacccaag
    10441 cctactaata caaaaatctc cgggccaacc cggtcggtca ggtatccgct taaaggcgcc
    10501 ccgacgacca tcatcaaggg aaagaccatc atcaacagtc cggcatagct aggactcaac
    10561 ccgcgagcat tttgcagata aaaaggaatc actacattaa caaagaagtt ggaagaaaaa
    10621 atcaacaagg ctgtaatcaa gctcatggta aaaatcttgt tcttaaaaat accgaaagaa
    10681 atcaatggag aggctgcttt cttttcaact tttaaaaagc tgacaaaggc aatcactgaa
    10741 attacgaaca gtacaagggg gactggatgg aaaaagccac tttcttgccc caaaaagatt
    10801 gttccaaaaa ggctgataat cgtagccgca atggcagcaa agccccctcc atcgattgta
    10861 tggccgctca tggtgatatc ttttggtaag aatttttcag caatcaacat cgttacgatt
    10921 cccaccggta cattgatcca aaaaatgtac gcccaagaaa attgggacaa aatcaagccg
    10981 ccaagacctg gtccagcaat cgagcctaat gaaacaagcg caccgattgc tcccaatgcc
    11041 cgaccacgtt catgtatggg aaagacttcc gtgatgattc cggcatttgt agccatagtc
    11101 atactagcac caatggcttg taccacccgt gcgaacaatt aaatacctag cgaatgattg
    11161 aacccacaca acagcgagcc caagacaaag atcagcgtac caatccgaaa tactttgatt
    11221 ttcccacagc tatcgccaat tttaccaaac agcaacaaac aagaacagac aatcatcaga
    11281 taaacggaca ctacccattc cgcttgattc attggtatgc ccatatcatc tgagatggta
    11341 ggtagtgcaa tattgacaat actgccatcc aatgtggaca tgaaggtaaa catagatata
    11401 gcaatcaaaa tccaccagcg atttttttgg acttttgcat cttcttgata actatgcata
    11461 ataggatttc ctttctaatg atcgtgtagc taagaacaat ataacctatg tctactgccc
    11521 aacaacttct cgcgactcta gccataggaa gcttgagtaa tttcgatttg ttattctcat
    11581 gtaaccatgt aagtttgaga tgctttattt gaacttactc tatagtaact agtgataatt
    11641 cgctttactt tcttctagca atttctaaaa gtcgacttca ttgttcttca gattaatagt
    11701 gtgagctatc tcgtgagacg taagaaaatt tccattctac tttttcccaa ttttttcata
    11761 tttatggtgt tgcacctatt tgttttccaa tttgcttttc aaatacattg ctatgatatt
    11821 caattccata ggattatact aaatgtcaat agtatgaata atatattata gatgtttata
    11881 tatcactgtt aattattatc cacaataatt aatgattacc tcgatatttg tcaatcaaag
    11941 gttgaaacaa tttattttca gcatcttttc taacttgagc tgcactctta atagaatcat
    12001 acgatcctaa atagtacctc ttttttttaa agttatatac gcaatccatt tctctttaga
    12061 acgataaact cccttaacac cacttgtatt atttttcggt aattttgacg ttagacactc
    12121 tattactgtc ccttcaacac atgtccttgt ttcttgcctt tttaaacatc cacaactttg
    12181 agtatgtcca aaaaataagt tactataact tatctcacac atactgccac actcacattg
    12241 acaaacccat tttgttttcc atgaacagtt attgtccaaa ggatactttg cagtaagtaa
    12301 tccgaatttt ttattttcaa tgtgatgctt ttttgatttc aaacatccac agctttttac
    12361 atcacctttt agaagtcgct ttgccgatac taactttttt ctcccacaca gacattgaca
    12421 ttcccaaaca acagttcctt cacttcgcag tttagttggc ttgattgcaa ctaacttacc
    12481 aaaagtttta tttgtcaaat ttttatattt atgcctattt ttataatttc cacatccaca
    12541 acttttggtt ctacctgata gtaagtttga tcgatataca atcgtttcat tcccacagtc
    12601 acataggtaa cgatattttt tatatccatt atgagtaatc tcctcaacaa cagtaaggtg
    12661 accatatctt tccttcactg cacctaccct cccaagaaag aagtaccata taatttcaat
    12721 taacttttaa taaatcagaa aaaaatattt tcaatttttc ttagtctgat tttatgacta
    12781 atttataata caagactgaa cgatacgtac acgaaatcct caagatagat aaattgtttt
    12841 catctagtaa aacctgctca aaatatgagc ataaagacgg caataataca ctcgatattc
    12901 gataatggac ttgttttatt tgtcaaactc atcgtaatcg agatatcaat tccagtctca
    12961 atatcctgat caaaggtcta agaatccaac cattttcttg ttactttgtc agattgatta
    13021 ccttttttag tctccgaata ataaggtttt cggatcaact aacatgctct gagaaagact
    13081 actatctttg caaatttcca aatgaaggtg tgggccagta ctattcccag tacttcctac
    13141 atagccaatt atttgtcctt gcaaaacatt atctccagtt tttactaggt attgttgctg
    13201 gtgagcatat aacgcggttg atccatcttc gtgttgtatc accacgtaat ttccccaaga
    13261 gtagtgaaac tctgctttaa caaccttacc agctttaact gcatgaatcg gctcaccctg
    13321 tgtagctgcc atatcaagtc ctcggtgaaa ttcatttcca cgatttccaa acaaactggt
    13381 tattgaataa ttatgaatag gcacttggta tccagtctta ctcatttgtg gacctttaac
    13441 ttctttatca tagttggtta aatcataggt tttaattaat tcatttagct tcaaatagta
    13501 ttgtgtatct gttgcatacc gtcctgtaag gaatttagtt gcttctcgat atgttttact
    13561 attagctttt aatgctcctg cataaaaatc tttatttcca ctaattccct tagttaataa
    13621 tttggcataa tcttcaaaac tttcctcata gttttcatac actctaaatt ttgattgggt
    13681 cgtataaagc gttccattac ccaaatcttc gtttgttacc ataatgacaa agtttccatt
    13741 ataagttcct tttatcccaa ataaattgta attcggtgcc tgagctaact ggctttgacc
    13801 actggcagat tctaagatcg cttgtgcaat cataacagaa gcatataaat catatttctg
    13861 accaattttt ctcgcagact caccaatttt cataataaaa ttttctacag attcattttt
    13921 gtcaaaatga atttcttcat taccagatag tattggttgt gattggaaag attctgttga
    13981 ttctatttgc tgtggtgtcg aaaaattttt tgtttgtcct tccgtttttg gcgcttcctt
    14041 ttttgaagac tctatatggt ttggtcttgt cactttcgaa gaagttattt cacttgtact
    14101 acttgaagtt gttgaagatt cggcacttga aatcgtacta ctttcacttg acgaagttgt
    14161 tgaagactcg gcatttgaag tcgtactact ttcacttgac gaagttgttg aagactcggc
    14221 atttgaagtc gtactacttt cacttgatga agttgttgaa gactcagcat ttgaagtcgt
    14281 actactttca cttgataaag ttgtcaaaag ttcactactc tctgctgtta catcggaatt
    14341 actcatttca gtcgtctcgg ttctttcttt tgcaattttg tatgttgaat tttcgcttgc
    14401 caacacatgg aaaggtgttg ctataaagag ttgggacatt aatataactg ctgatgcaag
    14461 ttttaaagat tttttcattt gatttacctc tcttttttct ctttaatagt gttttctatt
    14521 aaaaattatg ataaatattt tcaatgaatt gagtaataca gtatatttct tgatttcatt
    14581 tagtaacgat tatgtttcaa tgtttaatcc tgttactgcc tttaaaataa aattataggg
    14641 ttggctaaaa ctaggatgaa ataaataatc taacattttc aatttgtcta atgtaatttg
    14701 ttcttctata gcaagagaaa acattgataa tagtccagat aaatcttctt gactacaaag
    14761 ttgtacacct actattctct ttgtattttt ttcaaagcta attcttaatt ttacacgact
    14821 attttttctc aaaaaaacag gtctaatgaa atcttctatt tctatataat ctatagactc
    14881 acttaataat cgtgctgttt ttaaattcaa acctgctgcg taaaaattgt aattaaatat
    14941 agaaaagcca tttgtaaatt gggaaccaga aggtgtaaaa tcttgattgt caatatgttg
    15001 agttgcaata taagctgttc ttagagcatt agaaatagaa aaatcaacac tacttctatt
    15061 aatggacgta aaaaaagttg tagcacaatc cccaatagca tatatatctt ttatatttgt
    15121 ttgctggtat tcattagtta gatatgctcc actaacatct ctcaagagtt cctttttgcc
    15181 aattggacta ttagatagaa atcctatagc ttgtacgacc atatccatag agtattgggt
    15241 accacttgta gtgactattt tttcaatctt ttttcttcct aaatattttt taggactttc
    15301 tgatagacaa agatgtattc cattttttga tagtatttct tcaactttat ctgaaaaact
    15361 acggtcgtaa taagtcgaca acactctatc tgcaacatcg aaaagatata cttctttccc
    15421 tctttttctt atggcttcag caatttcaac tccaatatat cctgctccaa taattcctac
    15481 ttttttaata gtattatcat ctaaagcttt aaaaatctct ttagcatttg gaaaattctt
    15541 tatcgaaaaa atattagcta aattattccc cggtacatct aacataattt gctttgaacc
    15601 tgtagctagt ataagtttgt catattcttg ggaaaatctt gtgccatctt tttttacaca
    15661 aataatcttt ttattttgaa tatcaatttt gtcaactgct gtctccaaaa acaacttttt
    15721 tatcttagaa tttagttctc ttctattttg ataaaaaaga tcttctaaat tgctaacttc
    15781 tctttttatg aacaacgatg tcccacattc taagtaacta atttctgatt ttttatctat
    15841 cagaataact tcataatttg aatcacataa gtttagaaat cgagctgcag ctagccccga
    15901 atggtttgat ccaataatta ctattttttt cacttaatca cattcttttc ccttctatga
    15961 aaagtatctt catttttaac taacaaccta tctcccaaaa taaaaaacaa aaagaggaag
    16021 aaaaagattc ttcctctttt cagagaagat ttaagaaatt gattttctat tcattctctt
    16081 tttttcgatt tttataccct aataacccta aagttcctaa caggattaaa ccaccactaa
    16141 tagtaaagat attactatca atctctcccg tcttaggata agcttttgag gactgctttt
    16201 gaggactgct tttgcctcta tttttgtttt gcttgctacc ttgattgtca gtcttattat
    16261 ctttactaac ggctttttca ctgtcttttt cgtttgaaga aacaactaca ttagtaggtt
    16321 cactagtatt accagattca tcagttgcag ttactatcac ttttgttcct gcctctaatt
    16381 taggaacttt aactgtaaaa ttaccatttt tatcagctgt cccatggtat tgactcccgt
    16441 ttggtaatgt aactgtcacc tctgcatttg gttcagtttt accagtaaca atttggtcac
    16501 cctctgtaat aggatcaact tgaggtttat tcggtgcttt tttatcttct acagaaatag
    16561 taacctctac ttcttcttta gatccatctg gatatgttac tactacaatc ccttttttgt
    16621 ttccagctgt actaacatct actttctctt tccaagtata agtagttcct tttggtagat
    16681 cttctttgtt cttaattccc tcagatgcgt ctggctcttt atttaagtct gtagtcactt
    16741 tttgtccttc aggatt
//
LOCUS       DACEOM010000070        15979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEOM010000070 DACEOM010000000
VERSION     DACEOM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.072"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            66..767
                     /locus_tag="IW781_002274"
     CDS             66..767
                     /locus_tag="IW781_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1183263.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IW781_002275"
     CDS             764..1885
                     /locus_tag="IW781_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1183264.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IW781_002276"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IW781_002276"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1183265.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IW781_002277"
     CDS             3264..4172
                     /locus_tag="IW781_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1183266.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IW781_002278"
     CDS             4207..4479
                     /locus_tag="IW781_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1183267.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IW781_002279"
     CDS             4490..5536
                     /locus_tag="IW781_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1183268.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IW781_002280"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IW781_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1183269.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IW781_002281"
     CDS             complement(8547..9218)
                     /locus_tag="IW781_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183270.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IW781_002282"
     CDS             complement(9211..9705)
                     /locus_tag="IW781_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1183271.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IW781_002283"
     CDS             complement(9725..10945)
                     /locus_tag="IW781_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183272.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IW781_002284"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IW781_002284"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1183273.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..13791)
                     /locus_tag="IW781_002285"
                     /pseudo
     CDS             complement(13021..13791)
                     /locus_tag="IW781_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(13953..15101)
                     /gene="tnpB"
                     /locus_tag="IW781_002286"
     CDS             complement(13953..15101)
                     /gene="tnpB"
                     /locus_tag="IW781_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1183274.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(15118..15522)
                     /locus_tag="IW781_002287"
     CDS             complement(15118..15522)
                     /locus_tag="IW781_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1183275.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            complement(15608..15835)
                     /locus_tag="IW781_002288"
     CDS             complement(15608..15835)
                     /locus_tag="IW781_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183276.1"
                     /translation="MEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQN
                     FINAEFNRIRQKSPILYRWVMNAVRYEGYRW"
BASE COUNT         5108 a         2928 c         3222 g         4721 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaatcta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttgtgaacta
    13801 ctcaccactt agctaaacct gacggttctt aacgcttgaa gtgggagctt cttgggaata
    13861 gagcatactt gatagcgtat ttctttacta acagcggtgt ctcttattta ccaagctatc
    13921 cccgtagttc ctacggttct tgatgattgg atctaagcca atcccataga atgtagtctt
    13981 agaccttcgg tcagaatatt gatactagca ttgatatcac gatcatgatg agtatgacaa
    14041 acaggacaag tccattctcg aatttcaaga gatttcttgc catctttatg tccgcactct
    14101 gaacaaatct gactagacgg aaaccattta tctactttga tgatttttcg tccataccag
    14161 tcagccttgt attgtaattt agtcacaaac tttgaccaag aaacatcaga aatactttta
    14221 gctaatttat gatttcgcaa catacctttt gtgtttaagt cttcaataca gataatatcg
    14281 tgatttttga tcatttctgt actcaactta ttcagaaaat cagtacgttg attcattact
    14341 ttttcatgca atctagctac tttgcgtttt tgtttttgat aatttttagc ttcaaataga
    14401 ttggtacctt tatttttagc caataaagca cgcctagaca atttgcgttg ttcacgcttt
    14461 agtttctttt ccattttgga cgtgaattta ttattatcaa ttttttgtcc atcagaaaga
    14521 atcgcaaagt ccataatacc taagtcaata ccaattgtgg aattagtttt aggtaattcg
    14581 ataatttctt ctttacacaa caaagaaata tagtatttac cgctagaatg acgtgatatt
    14641 gtagcagatt tgataatccc ttttggttgt ctatgaagct taattcttac taatgacttc
    14701 aatttaggaa ctttgatgaa tttactatca atcaaagcaa ctgtaccatt ttgattattc
    14761 gttgtataac tctgaacagg atttttctta cttttgaaac gtggaaatcc aacggattta
    14821 tcccgaaaaa aattcttgta tgctttatct aaattaagtt gggcattggc taaagcaagg
    14881 ctatcaattt ctttcaaaaa cggaaattct ttcttgtatt tagctggtgt cggaaaagtc
    14941 atttttttag aagaatcatt tttaacttct tcatacgcct tttttcggtc atcaagcatt
    15001 agattgtaga ccttacggac acaaccaaaa gatttggcaa agaagatttc ttgttcctct
    15061 gttggataga ttctgaattt gtatgctttt agttgttcca taaagttcac ctactttcta
    15121 tttatgattg tttccttgat tttggatata cttcttaatc acatcaattg gtgcgccacc
    15181 agttgtcaga agacaaaaac ttttagacca gaacatttct ttccaaagaa attgtttgac
    15241 tcttggaaag tcacgtttta tcaatcttga gctggcactt ttataggcat tgatgaattt
    15301 tgtcaattct gttttaggtt gagctttgaa cataatatga acgtggtctt tatcatgatt
    15361 ccactccact aaagtaatgt gatacgattc tgaaattctt tcaaaagtag ttttggcata
    15421 atcagatatt tcatcatcaa tcacttgtct acgatatttc gttactaaaa caaggtggta
    15481 ataaagaaga aatactgaat gattatttgt atctaattcc atgttatatc aactcatttc
    15541 ttgtatagac tgattgtaac atggaacaaa ataaaaaggc aatacgcctt agttgtcggt
    15601 cttcgagtta ccaacggtaa ccctcatacc gaacggcatt catcacccac ctatagagga
    15661 tgggcgactt ctgcctaatt ctgttaaatt cagcattgat gaaattttgt aaaagagcag
    15721 gggagagatc cgtaagtttt ttctttccta aataaccgtt tatatgattt ttgatggtaa
    15781 atcggtagtt ttcataggta ttgtatttta gatttagttt aacgtattcc tccatccaaa
    15841 aatcaaggta ttgttttact cgagtatccg tacctaaaaa gtattgtcct gtttcgtcaa
    15901 tatctgataa aacttttcgt aaagcagctt cggcctctgg tcgggtgtct ccgccaactt
    15961 tctccacttt ttttcttga
//
LOCUS       DACEOM010000071        15924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEOM010000071 DACEOM010000000
VERSION     DACEOM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.073"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..263)
                     /locus_tag="IW781_002289"
     CDS             complement(<1..263)
                     /locus_tag="IW781_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183277.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDY"
     gene            complement(483..849)
                     /gene="ssrA"
                     /locus_tag="IW781_002290"
     tmRNA           complement(483..849)
                     /gene="ssrA"
                     /locus_tag="IW781_002290"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1020..1793)
                     /locus_tag="IW781_002291"
     CDS             complement(1020..1793)
                     /locus_tag="IW781_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1183278.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1937..2287
                     /locus_tag="IW781_002292"
     CDS             1937..2287
                     /locus_tag="IW781_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1183279.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2268..2984
                     /locus_tag="IW781_002293"
     CDS             2268..2984
                     /locus_tag="IW781_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1183280.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2984..4432
                     /locus_tag="IW781_002294"
     CDS             2984..4432
                     /locus_tag="IW781_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1183281.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4503..5012)
                     /locus_tag="IW781_002295"
     CDS             complement(4503..5012)
                     /locus_tag="IW781_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183282.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5002..5727)
                     /locus_tag="IW781_002296"
     CDS             complement(5002..5727)
                     /locus_tag="IW781_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183283.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5950..8070)
                     /locus_tag="IW781_002297"
     CDS             complement(5950..8070)
                     /locus_tag="IW781_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1183284.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8300..9478
                     /locus_tag="IW781_002298"
     CDS             8300..9478
                     /locus_tag="IW781_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1183285.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9494..10252
                     /gene="aroD"
                     /locus_tag="IW781_002299"
     CDS             9494..10252
                     /gene="aroD"
                     /locus_tag="IW781_002299"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1183286.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10275..10760
                     /gene="ybaK"
                     /locus_tag="IW781_002300"
     CDS             10275..10760
                     /gene="ybaK"
                     /locus_tag="IW781_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1183287.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10831..12084)
                     /locus_tag="IW781_002301"
     CDS             complement(10831..12084)
                     /locus_tag="IW781_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1183288.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12201..13550
                     /gene="gdhA"
                     /locus_tag="IW781_002302"
     CDS             12201..13550
                     /gene="gdhA"
                     /locus_tag="IW781_002302"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1183289.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13662..15008
                     /locus_tag="IW781_002303"
     CDS             13662..15008
                     /locus_tag="IW781_002303"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1183290.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15316..15702)
                     /locus_tag="IW781_002304"
     CDS             complement(15316..15702)
                     /locus_tag="IW781_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1183291.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15751..>15924)
                     /locus_tag="IW781_002305"
     CDS             complement(15751..>15924)
                     /locus_tag="IW781_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ1183292.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         5212 a         2850 c         3195 g         4667 t
ORIGIN      
        1 aaatagtcat aacgccgatg atcgctccgt gcgtctggca catcagtcac gatatcaaac
       61 aaaaaataca catctttctt cattctggtt tttgttgccg gaattttaaa gtagttctta
      121 ttagagtaat agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa
      181 tcccacacct tataaaaatc tttgacaaat ctattcgaag gatcaaattt aaaccataat
      241 tgtgtttttc cttccatcaa cataatgttc acctcaaaaa gagtatacga actaatgttc
      301 tttttgtaaa gtaggaatat cattgataat atggatggaa catatatatt aaaaagtgtt
      361 ctttttgaat gctattttga atgccatttt cattcattta acgaaaatct gcgaaaataa
      421 aaaatgctca aacccttata aaataaggat ttaagcatct agcgaaatct gctgaaatct
      481 cacaatggag acggcgggag tcgaacccgc gtccaaacac atcgccactt aaaaatctac
      541 gttcatagtc aactcattta aggattcact ttacagcttg ccgagtgaca ggcattctgt
      601 atcgctagtc tgatgttctc tttttcaact tacagacgga aagttgaaac gtatcccact
      661 aaagttagga cccttactcg agcacatggg cgatgccgag aggatctacg ctaactgttt
      721 ttaggcagct aaagcgtaag agttgttttc gtttttagca gttatattta actgtaacgt
      781 ttttacgtag acgtaaccta cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt
      841 aacgtccccg ttagctaaca agggttacgt gtatttttca tacaaaatta gtatagcata
      901 tattgggata aattcaataa aaaagcgaat catatcccat tatttatata ataactgccc
      961 ctttcttaag aaaaaataag cgattttctt aagaaaagag gtagcttcaa aaaaagatct
     1021 taaatcagat caaaatactt caatgcgtgg acaattccgc cttctgtatt tttctttgtg
     1081 ataaaggtag aaagttcttt taatccgtca taggcgttgc ccatagcgat tttgttgtcg
     1141 caagcttcca acaaggcaaa atcattcgga ccatcgccaa atccaaaagt aggaactttt
     1201 tccagctgaa gttctttttg cagacgtttg acacctgccc cttttgagat tcctttcttc
     1261 actgtatcga tcgagtaagg accattccga taaaaagtta gctcaggaaa atgttcgtga
     1321 tagtattcgt cacctgattg agataagatc agcaacatat ttactgtgtt ttctttaaag
     1381 cctaaaggat caatttccgg aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt
     1441 tggctatgac cggtacacca gattttctga tcgttatagt aagaaagttc atgaccgttc
     1501 aactgtacat gatcatacat tttttgacat tcttctgaag aaaatgtttg agaatagatt
     1561 tcttcgccgt ctactcgaat gaaagaacca ttcatggtga ccgcagaagt aatgcccgca
     1621 tcttccatga tagagtggat ttctgcttct gtacgtcctg tagcgatgac ggggagaaca
     1681 ttgttttctt ttaatgcgtt catagctttt gccacttcgg gtgtgatttt tgatttcgca
     1741 tctagtaatg ttccatcaag atcaaagaaa gtgattgcac gataatttga cacaaataaa
     1801 actccttttt tattggaata agtttatctt aacaaagaat gaacaaaaga caaacctttc
     1861 gtgaagaatt cctgaacata ttggttcagt caacaattta gagtatacta taagaagaga
     1921 atttaggagg gaaagaatga tttttttcag tattggtatc ttattcttaa taattggcag
     1981 tctttttcgg attctgccct caaaaggaaa tctgcctttt tatgggtatc attcgcctct
     2041 tgccgcaaaa acagacgctc attggcggct ggctcaaaag acaagcggaa actggttttt
     2101 tcttatgggc ttactgatgg cattgatcgg ctattattta aagacatctg ggcatacgaa
     2161 ttattttctg attgaaatgc ttctgctagt atttccaatt atgcctattt ttattatgac
     2221 agaaaagaaa ttacagaaat atgacctgga aactggaggg aacgataatg aatattttaa
     2281 tgattgaaga taatcaatct gtttctgaaa tgatgcagat gtttttctta aatgaaggtt
     2341 gggaagcaac tttcaagtat gatggaaaag aagggctcga tgccttttta gaaaacccac
     2401 aacattggga tatgattaca ctagacctga atttgcccac actggacggt atgctagtag
     2461 gaagagagat ccgcaaagtt tcaagtactg tgccgattat catgctgaca gctagagatt
     2521 ctgaaagcga tcaggtgatc ggcttagaga tgggggcaga tgattatgtc acaaaacctt
     2581 tcagtcctct gacgcttatt gctcgaatga aagctttgca tcgccgtagt gagttggcag
     2641 aagcaagaga agaagcagta gtttcagatg atcattttga tatcgagact agtcatttca
     2701 agatgaatac gaaaacacga gaagcgtatc tggacaaccg gccaattgaa ggactgacgc
     2761 ctaaagagtt cgatctattg tttactttag caaaaaaacc acgtcaagta ttctctcggg
     2821 agcagttgct tgaattagtc tgggattatc agtactttgg tgatgaacgg acagtggacg
     2881 cccatatcaa gaaattacga caaaaaatcg aaaaggtcgg accacaagtc atccaaactg
     2941 tttggggagt cggctacaaa ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca
     3001 gcaactctta gctttctgcg gaatggtcgc gttgatctta ttgatcgttg gagtttcttt
     3061 tacacaactg acaaaacaaa cgattgaaga aaacaattac cagcaactgt ttggatatgc
     3121 tgaatcagtt gaaaagacaa cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga
     3181 tcaggcgttt caaaatgcat tgctgctgac agagcaagta ctgacagaac aaaatgtgaa
     3241 ctttatattt atcgataaaa acgaacgtgt catttatccg actaatacag ctttactgaa
     3301 ttttacctta acagctgatc aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac
     3361 atcgaataaa aatattttag gacaagcaca ggcgacctct tacgcacttg tgccatttaa
     3421 tttgaatcat gaattctatg gagcacttgt agttagtcaa cctgcgcgaa atatcgataa
     3481 cagcgttcgt ccaattacac tgaacctgtt caaaggattt attttttcca gtatcgttgc
     3541 agttattgcc agctatgctt ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa
     3601 tgcgacgaaa gaagtgacga atggtaattt tgatgtacag ctccccgtac atgacaaaga
     3661 tgaatttgat gagttagcag atgatttcaa taaaatgacg aattcattga aagaatctca
     3721 agcagaaatt gaagaacaag aaaatcgtcg aagacaattt atggcagatg cttctcacga
     3781 gatgagaaca cctcttacga caatcaacgg acttctggaa gggttagaat acaatgcaat
     3841 ccctgaaaat cagcgagaaa acgcgattaa attgatgaaa aatgaaacag agcgtctgat
     3901 ccgtttggtg aatgaaaact tggattatga aaaaatccgt acgaatcaaa tcagtatggt
     3961 cattaaaaaa ttcaatggaa cagaaacatt gaaaaatatt gttgcacagt tagaagctaa
     4021 agcagaagca gcaggagata cattgacatt gaaagccgtt gatgatattg atgtttacgc
     4081 tgattatgac cgatttgtcc aaatcatggt caatattatt caaaacgcga tacagtttac
     4141 tgaaaacggc caaatcacga ttactttaga aaaaggctat cttgaaacga tcatcacgat
     4201 tgaagatacg ggaattggaa tgtctgaaca acaaatgaaa agtatctggg atcgttatta
     4261 taaagtagat ccttcaagaa aaaatacaaa atatggtgaa tcaggattag gtttaccaat
     4321 cgtgcagcaa cttgtacgct tgcataaagg aaaattagaa gtagaaagcg aactaggaaa
     4381 aggaacaaaa ttcgctgttc gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag
     4441 cggtataaaa gatcatgaat cgatcttcta taccgcttgt tttttactat aaaatgattg
     4501 atttagcccg cgggctctaa acgatcaata taaggacttt gagcaatagt agcatcatga
     4561 gtaaagagta agataccttg attttcttca tgagctaaat aagaaagcaa gtggagtagc
     4621 tcttggatct ccaatacact aagttcatca aatgtatctt caataacaat attgtttcgc
     4681 ttggaaagca aggcatgaag taattgcagc tttacttttt caaaagacgt caactcagct
     4741 gccgcacaat taagcaagga aggagagata tcaagttttt cttgataggc caaacgatct
     4801 tgtttgattt ctttttccgg tacatccaac aggacatggt ctttaagatt cagataagga
     4861 aacatcggcc aatttttttg gatgatcgca aaattatcca gttttgcaac aagaacctct
     4921 tttttcaagt gctccaatat tttatgctgt ttttcgagag gacacaaaat aacggtcaag
     4981 tgttcagcat gaatcaaata ttcaggtttc attacgtcca ggcctcctac tttgcaaatg
     5041 atggataaaa aaagtgaaag ggaatagact aagaatgaga cagcatccac tgagcccaat
     5101 aagagtttgg gcaaatccct taagtatatc tgtgaaaaaa agatcatttt ggaaatcgat
     5161 ggtaaaaaaa gtctgttgat ccatcggaat tgtaagtccg cttaccgaag tcaagatatc
     5221 acggatatct ggcagttcag tagtctttaa gacaagaaaa ttgatccttt gaagaagcgc
     5281 ttcataaact gactggaagc ttacgatcca caataaaacc aagatacaac aaagaaagag
     5341 aggaaagaac agctgccaga aaataaaaag gaataatttg gttttagaaa gaccagtcag
     5401 gtgccaattg atcaattcct gcttgttttg cctgactgaa tagtaaaaga aaaagataaa
     5461 aaggaaggca aatagaaaaa ataaactgaa tatgcctttt atataaaatc ggctagctaa
     5521 ttctaaagcc ggaagaagct ctgtagatgc agaagcgcca ataaatgaaa gccgatcttg
     5581 gacttgattg gctaaagccg attgcaaatg gtaaagagtc aggcatgcta tcaataaaaa
     5641 agcagaaaag cttgaaaata aacaatagag tattgtctgc tttttgtaat aaattaatcg
     5701 agcagtagca tagcggatta ttttcatact tttcacctct ataaaaagta tagaaaatga
     5761 atatgaataa agtatgaaat tcctttagtg aaatcacgaa ttaaaaccag aaaatatttt
     5821 gtaaaaagca agaggttgtg aagaagctgc cctattctat taccgtagat gcaagctttt
     5881 gaacactgtt tattcgaaaa gaggagcagc gacaataatt ttgtcgctgc tccttttaaa
     5941 tttattttat cattcggaag aacttgtggt cgttccgtga agttcttggt acgtttgcgt
     6001 tttataaaga tcaacagttg attgtccgtt gtgttcacta aagacactag ttgacttgtc
     6061 accaagagat ttttcagctt cttttaattt ctctaagcca ttcttgtagt cataatcact
     6121 ggtatcaatt gggtctaatc cactattcgt atagaaccga agaagatcac cattattgat
     6181 ttggtcagaa acagctaact gtttatcaac tttttctttc agtgaatcaa tttcattctg
     6241 tacttcctca gtaggttcag taatagcttc accagtttcg ttactataga tcgtaccacc
     6301 gtagtaagta tattttggtg taacaaaatc accatcacgg aaagaaacaa gctgttcatg
     6361 atctttcgag aatagatctt gtcctaactg aatataattt tttgtatcta cacctagtaa
     6421 gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa gtgtgattga ttccgccctg
     6481 tgtttgtcca gggatatgga tcatataagg gacacgctgc atttgtgcat tgtcgtagtt
     6541 tgtccaagta gaacttgttt ttccaactaa ttcagctaga tttttatttc ttgaattcga
     6601 gacaccgtaa tggtcaccgt aaaggacgat gactgaatta tcatacaagc cgctttcttt
     6661 caaataattg aagaattctt gtacggcggt atctaaataa ttggctgtcg caaagtaacc
     6721 gttgatcgtt tcatcatttg tattggctaa cgggaatcct gcttcgtcat tggtgaattt
     6781 tgaataagga taatggttag atacagcaat gaattttgaa taaaatggtt gttgcagacg
     6841 ttccaagtat tgtacggatt gttggaaaaa tggtttatca tgcaacccgt attggaacga
     6901 attgtcatca ttaacatcat aataagaaga atcaaagaaa taatcatatc ctaaattctt
     6961 gtatgtctcg tttcgattcc agaatgtacc agcgttccca tggaataccg cactagtgta
     7021 accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa gtattttttc cgcctaattg
     7081 tgtgaacaag gagccttgat tcaagccaaa caatgaattt tccatcagcg tttccgcatc
     7141 gcttgttttt cctgctttta cttgatggaa aaagttgtca aaactgaacg tgctgttgct
     7201 gtggtaaaga ctattgatga aaggtgtaac ttcatgctgg agaccattct catctgtcaa
     7261 tttataatcg atcaggaatt gctggaagct ttctaaatgg atataaatca cattttttcc
     7321 tctagcgagc ccgaacaaat cgtcatttgg tgctgcataa tgttctttta cataattttc
     7381 tacttctttc atatcattgg gacttgcttc tgcccgcact tggtttgtct ggtaagtttg
     7441 aacaccgtca taaaccatga aagcattcaa gccaagatat ttgaccaaat aatcacgaga
     7501 gaatgttcga gtcagcaatt ctggacgatc agcttccgcc ataaaaagat ttcctgagaa
     7561 aatcataact gctaatgtag agatcgcaaa agccattctt gctcggatcg ggcgagggtc
     7621 caacttgatt ttttttgcaa tcagcagaac agtcaagata ataaaatcga tccaatagat
     7681 cacatcatat gggcgaaaca gtcgaatggc actttctccc agaccactcg ctactttccc
     7741 agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat aagtgacatt
     7801 cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc atcgtgatat aagcaactct
     7861 agggcgtctg atataaagag caattccaag taataacaat gtagttgcaa tagggttgat
     7921 aaacaagata aagtattgaa tgccactttc gattccgaga tggaaatcaa ctaaataggc
     7981 aaaaatcgtt ttcaaccaaa gtaaaacgac tagtaaagaa aaaaatccga gcctcttatt
     8041 caaaaaggca ggtgttttta tacatttcaa gataatcttc ctttctgcac ttcttaatca
     8101 cagaagtgtc ctgaaaccat gttacacagc tcacttattc tcatttattt tactagtatg
     8161 acaatatagt gtcaattgaa accagaagtt tatgctgttt cattaaggtt ttctttaagc
     8221 ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt tgttatactt gggaagagaa
     8281 agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca atcgattgaa
     8341 aaaaggatat ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag aaaccacgca
     8401 atgggtggaa ttagttgatt cgcaaaacca atttatcgca aaaggatatc taggaaagca
     8461 gaataaggga atcggttggg tattgagcca gcagaacatc ccatttgatc aatctttttt
     8521 tgaagaattt tttgtgaagt caaaagaaaa aagaactttc tttttttcag atgattctac
     8581 taccgctttt cgtatattca atggagaagg agacgggatc ggcggtctga ttgttgatta
     8641 ttatgcaggt tatgctgttt tttcttggta taacgaaacg ttatatgcac atcgtgaatt
     8701 attgattcaa gcttttcgtg aaatctaccc ggaaatcata ggaggctatg agaaaattcg
     8761 atttgaatca gtaaatctgc ctgagagcca gttcatttac ggaaaagagg caccggaacc
     8821 cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag gattaatgac
     8881 tgggatcttt ttagaccaaa aagaagtacg gggatcatta gcagaaggtt tcgcttctgg
     8941 gaaaacagtt ttgaacatgt ttagttatac cggcgccttt tctgttgctg ccgcaatggg
     9001 aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa aaacacagga
     9061 acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta tggacgtatt
     9121 cgagtatttc aaatacgcca aaagaaaaca atttttatat gacatgattg tattggatcc
     9181 accaagtttt gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt atggggaact
     9241 ggtcaaggat agtctggata ttttagcaga agacgggata cttattgcgt ctacaaatgc
     9301 tgcgaatgtc tcgatagaac aattccaaac aatgattgaa gaggaattcc atgccaaaaa
     9361 tgtttctttt gaacaacttg ctttatatcg tctgccaaaa gatttccaga cgacggatac
     9421 ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata accattagaa
     9481 ggagcgatgc agaatggttc gggtaaaggg attgaatata ggaagcggta gaccaaagat
     9541 ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag ttcttgcacg
     9601 tgaagcaggg gccgatttag tcgaatggcg agtcgatcat tatcgtgaag ttttttcttt
     9661 tgatgaagta gcagaaacat tggttcttat ccatgagatg cttgatggac tgccgttatt
     9721 atttacgttt cgtaccttag ctgaaggcgg agaacaagac atatcgatcc gtcagtatcg
     9781 cgacctttat gaacatgtga tacacacagg attagtagat atggtcgata tcgagttgtt
     9841 caaagtagaa agtctaggaa aaagactgat agaggaaatc aagtctttaa acattccttt
     9901 aattatcagt agtcatgatt ttaaagaaac accagcagac cctgtcttgc tttaccgatt
     9961 gaatgttatg gaacattttg gagccgatat cggaaaactg gcagtaactc ccaataatga
    10021 acgagatgtt ttgcggttga tggagttgac tagacgggca aatgcttttg tctccatgcc
    10081 tattattacg atgtccatgg gaaatcttgg taaaatttct cgtctggttg gcggaacgac
    10141 gggttccgtt atgacatttg gttccttaag cagcgattct acttctgctc caggacaatt
    10201 accggtcaaa gcattgaagc aaattattca attattaaat gaaccgttgt agaacaagtt
    10261 tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc cgtatggtcg
    10321 aacaaaaaaa gattccctat acagagcacg aatatgagtg ggacgaaagc cacctaagtg
    10381 cgtcatcggt cgctgaacag cttccagaaa gccaatctcg tatatttaaa acactggttg
    10441 ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc gaattgaatc
    10501 taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga aatgctccat ttaaaagatt
    10561 tggaagcaac gaccggatac atacgtggtg gctgctcgcc tataggtatg aaaaaactat
    10621 ttcctactta tttagatcaa attgcagaaa gctatgaaca aatcattgtc tcggctggca
    10681 gaagagggct acagatggag ttagcacctc aagatatctg tgcattaacg agcggacaat
    10741 ttgcagatat aaaacaataa atcatacgta taaaagaaag aaggaggccg ggatgttgtt
    10801 tgtccagtct ccttctttct tttttataca ttatgctctt tgcccggcat tcattcctgc
    10861 tacatgccct gtagcgaaag ccgctgtgat attaaagccg ccagtataac cattgacatc
    10921 cagtaattct cccgcaaaat acagaccatc aatgatttta ctctccaatg ttttaggata
    10981 aatttccttt agagaaaccc ctccgccagt tacaaatgat ttctcaatag gaaaagtttt
    11041 gttgattgat agcttaaact ctttgcatag ttgaacaaaa tcttgaattt gtttttgaga
    11101 ggcttgttgt cctgtcagat gatccatttc aagccgctca agaaagaaaa ctaacaatcg
    11161 ctcaggcaat ataccatgcc aagcattgac caagttcttt ttggttgctt tacttttttc
    11221 tgtcaatata tgaataagct cttgtttggt ttgtgtaggg aaacaatcca gagaaagtgt
    11281 cactggttct cccgttttct ctaattcttt attgacaaaa cttgaacaac gcagtgcagc
    11341 cggaccagaa ataccgaaat gggtgaaaag taaatccatc gtatgttcag tcaatacacg
    11401 acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt gaagtgtttt
    11461 ctcctgaata tacggttcat ccgatatcaa gggggactct gtcgcataaa gaggcgtcac
    11521 cgtgtgaccc actcgcttca cgatcttgta cccatctcca gtagaaccag tagatggata
    11581 agtccgtcca ccagttgtta agataatggc aggagcatgg aactcttcga attccgtgcg
    11641 tataccgtaa attttatgtt ctttgtggat caatttctct actcgagtag agaatagaag
    11701 cgtcacatct agttctttca gacgattgag caatgcttcg ataatcgttt ttgatttatt
    11761 tgttacagga aacatccgtc catgatcctc ttcttttaaa tgtacgcctt gtgattcaaa
    11821 gaagttcatg atatcgaaat tattccattg cgcaaacgta ctatataaaa actttccatt
    11881 tccaggaata tgtgcaatca aatcatcaac aggacgattg ttcgtaacat tgcaccgacc
    11941 accacctgtc atcagtagtt tttttcctgc ttttttgttt ttttcgatca agagtacgcg
    12001 tgcgccttgt tcagctgcac tgatcgctgc catcatcccg cttgttccag cgccgactac
    12061 gatcacatca tacagttttt ccattccctc acctcaagga aagtgtagca tatctgaaga
    12121 aagaaaacag aaaaaaatgt tttgagccaa aagagaaaat gagctaaaat gagggaagat
    12181 aagcaaagga gcgtggaacg atgacaaacg caacagaata tgttcaagca attcaagaaa
    12241 agctacatca aaaggatcaa gggcaagaag aatttttaca agcaatcgat gaattcatgc
    12301 ccacggttat gtcttttttg gacactcatc ccgaatatat cgaaaagaat atcttagaac
    12361 ttttaacgga accagaaaga gttattcaat ttcgagtgcc ttggcaagat gatgaaggaa
    12421 actggcgagt aaatagaggg taccgtatcc aatacaactc agctatcggc ccttataaag
    12481 gtggattgcg cttccatcct agtgtcaatc taagtatctt gaaatttttg gcttttgaac
    12541 agattttcaa gaatagttta accggtctgc caatcggtgg aggaaaaggc ggaagtgact
    12601 tcgatcctaa aggaaaatct gataatgaga tcatgcgttt ttgtcaaagt ttcatgctgg
    12661 aattgtcaaa acatatcggc ccatcgattg atgttcctgc gggagatatc ggtgtaggag
    12721 ctagagaaat cggctatttg ttcggcgaat ataaacgatt gaaaaaatat gatactggtg
    12781 tgttgacagg taaacctttg gatttttggg gaagtaagat acgtacggaa gctactggtt
    12841 atggattagt gtattatgtc aaacatctat tgaacgaaga aaaagattca tttaatcaaa
    12901 aaacagtttt tgtttccgga agcgggaatg tagccattta tgcaatcgaa aaagtgcaag
    12961 agctaggcgg gaaagttgtt acatgttcag attctagcgg gtacatctat gatccagaag
    13021 gaatagatgt gaaactattg aaagaagtca aagaaatcaa tagacaacgt ttgacggaat
    13081 acgcaaacca acgtccttct gctatttatc atgctggaga atctgtttgg acattgaaag
    13141 aaaaagcgga tatcgctttg ccatgtgcta ctcaaaatga aatagacgaa agcttggccg
    13201 aaatcttagt agaaaatggg atcaaaatcg tagcagaagg cgctaatatg ccatgtacag
    13261 taaaagctgt ttctgttctt catgaagcag gcgtgtggta ttgtccagga aaagctgcta
    13321 atgctggagg agtagcagta tctgctttag aaatgagtca gaacgtccag cgattgactt
    13381 gggaaagaga gcaagtggat gagcagctgg atcaaatcat ggaaacgatt taccatacgt
    13441 gtcgccaaac agcagccgag tacggcgacg aaaaaaatct gcttcttgga gcaaatgttg
    13501 ctggatttga aaaagttgcc aaagcaatgg cagcagaagg tttagtttga aaaatgaaac
    13561 tttcttggaa atattgtcag cattttgttt atttgcttga aaaataataa aaaaaaacgt
    13621 aaagtattac ctgtagagta aacctataag gaggagaaca aatggcacac attcattttg
    13681 actattcaaa agtagcaccg tttgtcaacg aacaagagtt agggtacatg caaagccaag
    13741 taacagcagc ccacaacgaa ttacgggaag gtactggcgc aggaagcgac ttccgcggct
    13801 ggatcgattt accaacgaac tatgacaaag aagaatttgc acgtatcaaa gaagcagcta
    13861 aaaaaatcca atctgattct gaagtattag ttgttatcgg tatcggtggt tcttatttag
    13921 gcgctcgtgc agcgatcgac ttcttgaatc acacattctt caatttatta gatagcaaca
    13981 caagaaaagc accacaaatc ttctttgccg gaaattcaat cagctcaaca tatattgctg
    14041 acttgatcga agtaatcggt gatcgcgact tttcagtaaa cgttatttca aaatctggaa
    14101 caactactga accagcgatt gcgttccgtg tattcaaaga gcttcttgtt aagaaatatg
    14161 gacaagaaga agcaaacaaa cgtatctacg ctacaacaga taaagcaaaa ggtgcagtta
    14221 aagtagaagc agatgcagaa ggttgggaaa ctttcgtgat tcctgatgac atcggcggac
    14281 gtttctctgt attaactgct gttggtttgt taccaatcgc tgttagtggt gcagatatcg
    14341 atgcattgat gcaaggtgcc gctgatgcaa gcaaagcata ctcaagcgac aaactagaag
    14401 aaaacgaagc ttaccaatac gcagcaatgc gcaacattct ttaccgtaaa ggcaaagtaa
    14461 ctgagttgtt gatcaactat gaaccaggaa tgcaatactt ctcagaatgg tggaaacaat
    14521 tgtatggtga atctgaagga aaagaccaaa aaggcatcta tccttcaagc gcaaacttct
    14581 caactgattt gcactcttta ggacaattca tccaagaagg acgtcgcaat atttttgaaa
    14641 cagttgtgaa ggttgaaaaa cctcgtaaat caatcacaat tcctgaacaa gcagaagatt
    14701 tagacggtct aggctacttg caaggaaaag aagtggactt cgtaaataca aaagcatttg
    14761 aaggaacatt gcttgctcat acagacggtg acgtgcctaa cttgctagtc aaaattccag
    14821 aaatggatgc ttatacattg ggttatacaa tgtacttctt tgaaattgct gtaggtattt
    14881 caggttactt gaatggaatc aacccattcg accaaccagg tgtagaagct tacaagaaaa
    14941 acatgttcgc acttctaggc aaaccaggct ttgaagatct tgctaaagaa ttgaacgaac
    15001 gcctgtaaga taagggtttt tcgctagatt taaataatca gtgatagaaa catgttcgct
    15061 ttaaaatgaa aactggtacc catttaggta ctcaaacata ctgcatcgtt tattcagaat
    15121 aaggggctgt gacaagacgt ttgtcacagt cccttgtttg tatagaaatc gattgattgc
    15181 cttgctcttt agtttgtttt aggactttaa gaacagtgag gctgacttcg aaggttaatt
    15241 ctgttttttt gttttctaga atcagtaagc aaaaaagcac tcacttcgag tgcttttttg
    15301 cgatatattc ttagcttaag ataggcctaa tttttctttt acagatgagg gtaccttatc
    15361 gctggtaatt acttttttct ctatgatccg cttttcgctt gctttcaact gcaagatcgt
    15421 tccttcgggg taagcagaac cattggcaga gtcaccagaa gtaatgacat cgaaatctaa
    15481 ctgtcttttt tcaccgtttt cagcataagc atctacgttg tatttaaaac ctcttccaat
    15541 tacttcccca ttatctgctt tgatatcagt ttctgcaggc attggagctt tgactaccgc
    15601 ataataatct ttacctacat aagtagactg atagtagtcc caacctttcc atcctataaa
    15661 agcaatcaat atgataacga tcaaccctaa aatttttttc atgaaagatc tcctttttta
    15721 tttttattat acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc
    15781 ttcaaaacgt cgttgtgctc gacttaatag aacactgtaa gccagtaata tgctttcaaa
    15841 tctactctct agccctttaa cagaacgtga gttgaaattt tgacaaccta atttttctaa
    15901 ggaagaaaaa acagtctcaa ctgt
//
LOCUS       DACEOM010000072        15730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEOM010000072 DACEOM010000000
VERSION     DACEOM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.074"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..522)
                     /locus_tag="IW781_002306"
     CDS             complement(52..522)
                     /locus_tag="IW781_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1183293.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="IW781_002307"
     CDS             complement(681..1139)
                     /locus_tag="IW781_002307"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1183294.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="IW781_002308"
     CDS             complement(1310..2455)
                     /locus_tag="IW781_002308"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1183295.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="IW781_002309"
     CDS             complement(2468..3385)
                     /locus_tag="IW781_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1183296.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="IW781_002310"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="IW781_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1183297.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="IW781_002311"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="IW781_002311"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1183298.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="IW781_002312"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="IW781_002312"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1183299.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /locus_tag="IW781_002313"
     CDS             6911..7792
                     /locus_tag="IW781_002313"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1183300.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /locus_tag="IW781_002314"
     CDS             7789..8505
                     /locus_tag="IW781_002314"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1183301.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="IW781_002315"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="IW781_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1183302.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="IW781_002316"
     CDS             9222..10223
                     /locus_tag="IW781_002316"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1183303.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="IW781_002317"
     CDS             10220..11581
                     /locus_tag="IW781_002317"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1183304.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="IW781_002318"
     CDS             11541..12692
                     /locus_tag="IW781_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1183305.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="IW781_002319"
     CDS             12667..13836
                     /locus_tag="IW781_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1183306.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="IW781_002320"
     CDS             14011..15390
                     /locus_tag="IW781_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1183307.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5139 a         2772 c         3346 g         4473 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa
//
LOCUS       DACEOM010000073        15400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEOM010000073 DACEOM010000000
VERSION     DACEOM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.075"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..563)
                     /locus_tag="IW781_002321"
     CDS             complement(<1..563)
                     /locus_tag="IW781_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1183308.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IW781_002322"
     CDS             complement(750..1400)
                     /locus_tag="IW781_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183309.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW781_002323"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW781_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1183310.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IW781_002324"
     CDS             complement(2912..3910)
                     /locus_tag="IW781_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1183311.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW781_002325"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW781_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1183312.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IW781_002326"
     CDS             complement(4926..5657)
                     /locus_tag="IW781_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1183313.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IW781_002327"
     CDS             complement(5661..6926)
                     /locus_tag="IW781_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1183314.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW781_002328"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW781_002328"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1183315.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW781_002329"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW781_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1183316.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IW781_002330"
     CDS             12268..13770
                     /locus_tag="IW781_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1183317.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IW781_002331"
     CDS             13772..14509
                     /locus_tag="IW781_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183318.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="IW781_002332"
     CDS             complement(14554..14727)
                     /locus_tag="IW781_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1183319.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="IW781_002333"
     CDS             14703..15368
                     /locus_tag="IW781_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1183320.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
BASE COUNT         4540 a         3297 c         2599 g         4964 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt
//
LOCUS       DACEOM010000074        14733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEOM010000074 DACEOM010000000
VERSION     DACEOM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.077"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(131..697)
                     /locus_tag="IW781_002334"
     CDS             complement(131..697)
                     /locus_tag="IW781_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1183321.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(697..1653)
                     /locus_tag="IW781_002335"
     CDS             complement(697..1653)
                     /locus_tag="IW781_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1183322.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1653..2309)
                     /locus_tag="IW781_002336"
     CDS             complement(1653..2309)
                     /locus_tag="IW781_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1183323.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2679..4697
                     /locus_tag="IW781_002337"
     CDS             2679..4697
                     /locus_tag="IW781_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1183324.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5124..7538
                     /locus_tag="IW781_002338"
     CDS             5124..7538
                     /locus_tag="IW781_002338"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1183325.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7886..9439
                     /locus_tag="IW781_002339"
     CDS             7886..9439
                     /locus_tag="IW781_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1183326.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9820..11604)
                     /locus_tag="IW781_002340"
     CDS             complement(9820..11604)
                     /locus_tag="IW781_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183327.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11619..13340)
                     /locus_tag="IW781_002341"
     CDS             complement(11619..13340)
                     /locus_tag="IW781_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183328.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13440..14201
                     /locus_tag="IW781_002342"
     CDS             13440..14201
                     /locus_tag="IW781_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1183329.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4787 a         2713 c         2925 g         4308 t
ORIGIN      
        1 ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga
       61 aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg
      121 cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt
      181 tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat
      241 caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc
      301 gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca
      361 tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga
      421 gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga
      481 cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct
      541 ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat
      601 cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca
      661 gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac
      721 atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac
      781 ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt
      841 caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac
      901 atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt
      961 tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt
     1021 gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg
     1081 cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata
     1141 atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc
     1201 tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag
     1261 acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc
     1321 aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac
     1381 gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg
     1441 gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa
     1501 agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat
     1561 cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata
     1621 tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta
     1681 tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg
     1741 agtaagtagc cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc
     1801 ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt
     1861 tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca
     1921 tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct
     1981 cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat aaatccagaa
     2041 acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca
     2101 aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc
     2161 agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac
     2221 gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca
     2281 gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta
     2341 tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct
     2401 ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa
     2461 tgcaaatgaa atgttagtga aataaaatca gtgtttttcg aaatcctttt aaacaagaac
     2521 gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat
     2581 gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa
     2641 ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag
     2701 aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg
     2761 tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc
     2821 aagcggatga aacacctgtt cagttcagcg caagaatcaa tacagcggca tttatcacag
     2881 aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga
     2941 tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt
     3001 ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg
     3061 aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg
     3121 ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt
     3181 actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga
     3241 cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt
     3301 acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag
     3361 gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa
     3421 actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa
     3481 tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca
     3541 atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga
     3601 cgaataattc cggtaacaat accaattctt caggaaatgc tggtacatca aacggaggac
     3661 aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg
     3721 atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt
     3781 atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg
     3841 gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag
     3901 cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat
     3961 acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc
     4021 caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag
     4081 gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag
     4141 gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca
     4201 cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc
     4261 aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag
     4321 cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga
     4381 tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga
     4441 tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag
     4501 tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa
     4561 cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa
     4621 tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt
     4681 tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat
     4741 ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg
     4801 aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg
     4861 agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga
     4921 ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc
     4981 aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga
     5041 aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt
     5101 tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc
     5161 aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat
     5221 tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg
     5281 aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc
     5341 tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag
     5401 gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt
     5461 ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata
     5521 aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag
     5581 tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg
     5641 ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga
     5701 aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa
     5761 gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta
     5821 aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat actctattcg
     5881 gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg
     5941 aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga
     6001 gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag
     6061 ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag
     6121 gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg
     6181 gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag
     6241 atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata
     6301 aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac
     6361 gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg
     6421 aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa
     6481 aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca
     6541 atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat
     6601 gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag
     6661 cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac
     6721 atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg
     6781 ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa
     6841 acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa
     6901 cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga
     6961 ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt
     7021 tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc
     7081 ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt
     7141 tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc
     7201 tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg
     7261 gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa
     7321 catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta
     7381 aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag
     7441 cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg
     7501 cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac
     7561 tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt
     7621 tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac
     7681 acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt
     7741 ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc
     7801 aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa
     7861 tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc
     7921 agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct
     7981 tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg
     8041 ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca
     8101 cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat
     8161 ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca
     8221 acagttttta cataataaac caacttctca agatgatgac tatctttatt ttgagaagac
     8281 ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact
     8341 tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca
     8401 tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata
     8461 cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac
     8521 aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca
     8581 catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc
     8641 tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac
     8701 agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat
     8761 tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg
     8821 ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc
     8881 tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat
     8941 tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg
     9001 tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac
     9061 tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca
     9121 aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta
     9181 tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca
     9241 gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac
     9301 gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc
     9361 ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca
     9421 agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca
     9481 agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga
     9541 gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt
     9601 gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca
     9661 agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt
     9721 attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga
     9781 ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact
     9841 attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc
     9901 gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg
     9961 gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa
    10021 tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt
    10081 cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt
    10141 ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg
    10201 tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat
    10261 attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg
    10321 acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt
    10381 attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg
    10441 accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat
    10501 tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc
    10561 taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat
    10621 cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat
    10681 tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc
    10741 acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat
    10801 gaaacgaatg gccggataga tagcaaagtt cataaattga gcacgtttaa aagcgttgta
    10861 ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt
    10921 gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata
    10981 ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact
    11041 gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag
    11101 accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac
    11161 ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt
    11221 gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa
    11281 tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct
    11341 taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact
    11401 gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat
    11461 tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa
    11521 tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt
    11581 gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca
    11641 aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt
    11701 tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca
    11761 acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct
    11821 gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc
    11881 aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga accgccttga
    11941 gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc
    12001 ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt
    12061 aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc
    12121 tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg
    12181 tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg
    12241 atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag
    12301 atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt
    12361 ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct
    12421 tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc
    12481 atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt
    12541 aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc
    12601 atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga
    12661 ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag
    12721 gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt
    12781 tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat
    12841 acataaaaaa caacagagat caatgtaacc catgttagta atagagaata acgaaacatc
    12901 atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca
    12961 atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta
    13021 aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt
    13081 tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt
    13141 gttcctagaa acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg
    13201 ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg
    13261 gcgatttgta aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag
    13321 aatgttttta agatagacat attattcctc cttttttcct gatacttttc gattctatac
    13381 cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa
    13441 tgaaaaaaat gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga
    13501 tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc
    13561 gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag
    13621 atataccgat caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga
    13681 cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa
    13741 aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat
    13801 ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg
    13861 aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca
    13921 gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag
    13981 acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga
    14041 tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat
    14101 ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca
    14161 gaactgaata ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata
    14221 actcgaaaat gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt
    14281 aatgtggggg attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc
    14341 tttaaaaatg gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa
    14401 aagtatggaa aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat
    14461 gagaagaaaa attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa
    14521 taggtgcata aaagcattac acttaaaata cattgttgct tacattatta caatatgtta
    14581 ataaggcaaa aataaataga tggattgaat aaacatattt tttaatcaaa tagtccctac
    14641 tgtgactttc ctcagctatc ggtgatacta ccgatagcat ttttttgctt ataaaataat
    14701 tataagtatt tagagatttt tcttctaaca ttc
//
LOCUS       DACEOM010000075        14214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEOM010000075 DACEOM010000000
VERSION     DACEOM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.078"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(48..461)
                     /locus_tag="IW781_002343"
     CDS             complement(48..461)
                     /locus_tag="IW781_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ1183330.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(830..1522)
                     /locus_tag="IW781_002344"
     CDS             complement(830..1522)
                     /locus_tag="IW781_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1183331.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1607..1783
                     /locus_tag="IW781_002345"
                     /pseudo
     CDS             1607..1783
                     /locus_tag="IW781_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1959..2528)
                     /locus_tag="IW781_002346"
     CDS             complement(1959..2528)
                     /locus_tag="IW781_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1183332.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2602..2925)
                     /locus_tag="IW781_002347"
     CDS             complement(2602..2925)
                     /locus_tag="IW781_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183333.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3079..3891)
                     /locus_tag="IW781_002348"
     CDS             complement(3079..3891)
                     /locus_tag="IW781_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183334.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4245..5093
                     /locus_tag="IW781_002349"
     CDS             4245..5093
                     /locus_tag="IW781_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1183335.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5238..6179
                     /locus_tag="IW781_002350"
     CDS             5238..6179
                     /locus_tag="IW781_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1183336.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6169..8340
                     /locus_tag="IW781_002351"
                     /pseudo
     CDS             6169..8340
                     /locus_tag="IW781_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8452..10623)
                     /gene="copB"
                     /locus_tag="IW781_002352"
     CDS             complement(8452..10623)
                     /gene="copB"
                     /locus_tag="IW781_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1183337.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10643..12829)
                     /locus_tag="IW781_002353"
     CDS             complement(10643..12829)
                     /locus_tag="IW781_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1183338.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12829..13038)
                     /gene="copZ"
                     /locus_tag="IW781_002354"
     CDS             complement(12829..13038)
                     /gene="copZ"
                     /locus_tag="IW781_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1183339.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13051..13491)
                     /locus_tag="IW781_002355"
     CDS             complement(13051..13491)
                     /locus_tag="IW781_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1183340.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13565..14104)
                     /locus_tag="IW781_002356"
     CDS             complement(13565..14104)
                     /locus_tag="IW781_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1183341.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4044 a         2920 c         2483 g         4767 t
ORIGIN      
        1 attgtacaat tagtattatc tgcactactt tctaattaaa taaattttta gttattaact
       61 tgattaaaat cagagataat atcatttaat tcagtttgaa gggttgtaac gattaaatgg
      121 aaattttctt catctgtttt attatatggg gatgttttcg ttatatataa caacttttct
      181 ttttcaattt tcattaataa ctctgtcgaa gtctgtactt tatcttgata attaaatgca
      241 gacataccac tttgtataat tgtaattatt gctgaagcaa ttgaaacaat caatagtaaa
      301 gacctgtgtt cggaagcctg atcaattaat attgggatac tagctgataa gactattttt
      361 ataatatttc cgataccaat tactctatta taaaatcgaa tttctttttt taatctatcg
      421 attgtagaat caatagattc gataaacgct ttttgatcca tcattttctc tccaatcatt
      481 ttattaaaat cattaacaaa aatattataa taggatttaa gtaagttcta actaatcaca
      541 tataaaaata aaaaaacatt tgtattttat caaagtttta ttatacttgt caatttgtgt
      601 acatcctaaa atataagcta tttgtttact tctgtagaat atatactttg ttgttagata
      661 agttgcacat gtttatagaa tctaaataat aaatttttat gtaccgcccc tcaaagtgaa
      721 ctgaacccca aaagttgaac tatttaatgg actgtttccg atattctact ggagatagtc
      781 cattaaatag ttcaactttt ggggttcagt tcaaagaggg ggggattttt tatcgttgtg
      841 gaatatttaa ataccaacgc ttgtcatgaa aatcttgtgc tccaccctta gtatttcctt
      901 ctggatcgtt tgtcgcacgc atcatgacgt atactttctt attagggaaa tcacgcatat
      961 tgaaagatac atgatagcca acatttccta aagtattata agcttgattt acgtctggtc
     1021 tataaatccc atcagctctt actctagcta gttcttttcc agtattgtag tccataatga
     1081 aaatatactc gtatttataa ttagcaatgt gccatccagc tacatgcaag tttgcgtttt
     1141 cgatttctcc gaactgatca atgtgggcgt aatttgttcc atctgtcagc gtagaatttg
     1201 cagcacctgc tcgagttgga tcaattactg gtttatcctc cgaagtagtt ggattatcat
     1261 ccgtaaaacc atgagccaaa tcataggcta gtttttcttt acttacaccc atttcagaaa
     1321 gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat aaatgcgatt
     1381 tgattcctgg ttggttataa ggagtgtcta atgttaatgg aataccatat ttcattgctg
     1441 aatctcttgc caattcaacg tatactttat agttcttttc aaaagtttct ttatcatgtg
     1501 tatgttgtaa ctcgatttgc acaggacttt tggctcacga ggttgtccta ctctacatct
     1561 tgctgataga tatgctgaaa ggaaaacacc aacattatgt tttgcttttt cagctagaac
     1621 gagacaagca ggaatacctt tagaagatat caaaggtttt ttaggtcata aagatgtttc
     1681 cactacacaa gtctatgccc atatttctcc cgaagtgaaa aaaagatcaa tgaatcaact
     1741 agaaaactat atagaagagc aaataaaaaa gcactcaaat tgagtgcttt tttaagctca
     1801 ctatcactaa aactatcact aattttattt ataagcgaac atgtttctat cactgatttt
     1861 ttaaatctag cgaaaaaccc ttatcttaaa ggcgttcgtt caattctttg gcaaggtctt
     1921 caaagcctgg cttgcctaga agtgcgaaca tggttttctt atagctcatg tagaaaaatt
     1981 ttcttgatga tgattttatc catcaattga gattgactgt tttgctctcc tatgatttca
     2041 gctatgattg tccgattttg cggttttctt cttaactggt agttgatttt cccagtttcc
     2101 atcaactcgc taagcatatc cacgactccg ccaaatccgg tctttttcgt aataaaacct
     2161 agtgctacca ttcgttcatc tagatttttt gctgtaatcg tcacaccagt aatgagcgag
     2221 tgctgtaatg tttgggccag ctcttccatc ccttcagcat tggatacgac ggaagctgtt
     2281 ttgttaatag gttcttcgtt tatttctttt ggttctttcc tctccacttt cactgtttga
     2341 cgaaggacat ctagttcttg caagagcgtt tcgtattgtt cttgggtcag catcacgcca
     2401 gctacttttt cacgattaaa tatatatacg ccagttcccg cgtctcgcgc ttgagcaaat
     2461 acatccatcg gcgatcgttt cacttctgtg atcgaagatg tgggaacttc taatttcttt
     2521 aattccaatg gatttcctcc tttactaatc gctttttatt atagcacaga ctgacttgtt
     2581 ttgatctttt ttccaaaggt tttatcggtt tttgcctaac cagtaattga tatcggtaag
     2641 aagtctttcg ataccaaatg aaggatggta cagatacgtt ttttgatctt cgttatggtc
     2701 tagtggaaaa aaatgatcga tgatcactag atctgcatac cgaggcggct tatttcgagt
     2761 acatacatgt atagagatcc gttttcgatg tcttagcgtt ttgttcagta ccccagcaag
     2821 tcttcttgct tcttggtgtt catagcgaaa aaaaaagacg attttcactt caggagggtg
     2881 gacatctaaa taccctcgtt cctggagtgt tttcagtaaa agcatccccc actctactgc
     2941 ggggtgtgca agtgcttgtt ggatcgctag ccgaaattga tatagtttat ctttggaatt
     3001 gggaaatttt tctaatgttt cctgataata acgaacttct tctatcagcc ggtcatggat
     3061 accaattacg taacgaagtc attcttggta aagtactttt gtcagctttg atttgctttc
     3121 gctctcaaat tcgctattca gccaaaacag cgaatcaagc ttttcagtca caatcgtcaa
     3181 tttccgatag tcttgtgtct cttctatccg tttccaccgc ttttcagtaa tctggttaca
     3241 gctcaagcgc aaaattgcta gataattttt ttcttttatt ggcaaaatct gctcctctct
     3301 aaatagcaat tcaggaaaca attcaaagaa tagtgttcga ctgtctccta aaatctcgat
     3361 ttctgccgga tcctttcttt gcttcaataa ttggatgaac atctcagaaa aggactgctc
     3421 tccatcatct gcaaaaaaat gcggaaaatc tttcaaaaag caaaacagca gatatcttac
     3481 ttttctttct gaaccaaaga tctgtgcttt tttataagaa aaagaaaggt ggtactcgcc
     3541 aaaccacttt ctcaatcgct cttgcgtaga acgatcgtac ttctgctcgt aaggttcatt
     3601 caatagcaga tttttcagta aacaaaacgg gggtgattgg atcagaaaaa caaacaagag
     3661 ataaggttcg ctatatcctt gatcataaac taactgaacg gttttcttat gaagaatcag
     3721 ctgaatggga cagtcgcttt ccgccaaacg tttcagatgc cgatccactg ttttaacatc
     3781 gattttcagt tcttcagcta aatcagcttt tcgccagatc aaatctttca ttagattgat
     3841 tagtgaaaat aaattctgtg cattttttga taatagtttt tgccatctca tcgatcattt
     3901 ctcacctgat ttctaaacat attgtttgat caaaatgggt tcactcctaa aaagataaaa
     3961 aactcccttt ctatttattt aaatacgcaa atcttcctca ttatttttta ttttactcaa
     4021 tttaatcgta ttctcatttc aaaacgaatg tactttaaaa aaacataata agatcacttt
     4081 gcttgtagga tgtattccct tccattacaa tgaaataaat agcgatttta tgagaaggtg
     4141 atgtgaaaat ggaagaaaaa atagaagaat ccaaacaaaa aatcgatcag ttgatataag
     4201 aattagaaaa agcggtcatc ggcaaacgtt caactatcca attgatgatc gtggccctat
     4261 tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt aggtaaaacg ttactggtca
     4321 aagcactagc aaaagctgta cagggaacct tcagcagagt ccaatgtact cctgatctgc
     4381 tgcctagtga tattcttggt ttttccgtat acaattcaca atcaaaagag tttgaatttc
     4441 gtccgggacc gatctttacc acgatcttgt tagcagatga gatcaatcgt acgactcctc
     4501 gtactcagtc agcactttta gaagcaatgg ccgagaatca tgcaacgatc gacaacaata
     4561 cataccctct agacaatcat ttttttgtct tagctacgca aaatccaatc gagtacgaag
     4621 gaacatatcc tttgccggaa gcacagctcg atcgtttttt attccgacta caaatcggtt
     4681 atccttcttt tgatgacgaa cttttgctac ttttggataa attcgaaaaa gtgttggata
     4741 acttaaatga agtattgaac agttatgaaa tagaagaaat aaaaacaaat gtggcgaatg
     4801 tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt agtgtcagca acaagaagtc
     4861 atgctgccat ccagcttggc atcagtccac gaggaagcct ttcctttatt caggcagcaa
     4921 aagcttatgc tttaatccat gggagaaatt acgtcacgcc caaagatttg caggatttag
     4981 ttccttacgt gttttcccat cgcctgattt tttacgatcg ttcattgaag gaagacgaaa
     5041 agaaacaaat cattcatact cttgttgcac aaattcctat tcctgtgagg tagattgagg
     5101 tagacaatgg aaaaaatcaa agattatttc attaattttg tgttatttct ggtatatttc
     5161 atcattgtgc tttttgctct tgtattcaac aattctttag gctggttctt gttggccttt
     5221 tttactcttt atgtatcatg gagcttgatt tcattaagca gctttctaaa gaaactttcg
     5281 gttacttgta cattgcagcc actcgtttat cgcaaaaaaa gtatgcaggt tattttttcc
     5341 atagcaaaaa agagaacttt ttctttacct ctccctcgtt tacaaatcgg cttcccagca
     5401 atctttgcag aaaaaaacaa gagattttga ttcttacaaa taaaccgcaa gaagtccaat
     5461 tgctttggca gccaaaagaa agaggatgcc atgaaacatt aactgtcatt tttacagcct
     5521 atgatagtct aggacttttc agaaaaagtg caaaaagaga gatctctttt cctgtgactg
     5581 ttctgcctgc tttttgtaag attcaagcag aaaaattaca gagagttctg gaaaaaaaac
     5641 agcagctgaa cgcgtaccaa agaggtcttg attttcggga acatcgcgct tatcgtccag
     5701 gtgattcttt caaccgaatc gattggaaat tgtctgctca tactcaagag tggttatacc
     5761 gtgaatacga ttatcaagaa gaagaatgtt ctcctctttt ttgcttttgg ggttcgccca
     5821 gtccaaaatt tgaacctctg ctagacttgt atttttcttt atggcatttg agaaaagaaa
     5881 agcagccgga acttctgata ttaggcaacc gcgcctttca tggcaaagac cccggtcata
     5941 cctattttgc ttctttggaa gctggcgatg agaaagcttt tttcgcacac ttgaaaaaat
     6001 atgcaaaaaa acaaatcgtc ctctttgttc ctgagaattc tcctgaagtc tcagaagcta
     6061 ttgaaacatt gtcaaaagat cgaacggttt atcttgttta tttcgtagaa aaacaactca
     6121 tggtccaaga aaaagacaag atatgttcat tgccaggagg tgaatggatt gacgattaaa
     6181 cgggaaaaat ggctgcgtgg cgtttactcc tttgtgatac taagtgtagg tgctccgata
     6241 tttgtagtag ctaatcaatt gcaaaacagc taccttctta tcctaactat tggtgtccta
     6301 tgccttattg tctgctgtat tcctaaaaaa tgggtacgtt tgctgctttc tttgccagta
     6361 atgataggtt gtttgttttt atattttcct tcgaagacat tttccatatt atggatctta
     6421 cagttttttt cgtcagtcac agaggaattc ttccatctat cccaaggtaa actttattat
     6481 ttgccagaaa agacagcttt ttttctcata atgttgctga tctatttatt tattacattg
     6541 accgtggatt acgactattg gtacgcaccc tttctttctt tgatggctta cttcatgatg
     6601 cttaccgttt ttcggaaaaa agatttactg tacgaaatga ttgcggtagt aactgttttg
     6661 ttcgtgtatc tggcagcaag gcaatttttc tccatccggt tgttgtcggg aaacactcga
     6721 ttccagtcat tgactacagt tctgcttatt tgtttcttaa cttttagcgc tatcttgcct
     6781 ttatatcttc cgaaagtaca ccaatctctc gaggaaacga gtgaaccgat ccgagaatat
     6841 cttaatgatg aagggcttta tgacacactc catgagtatc aattaaccgg aagtgcaaga
     6901 acaggcttca gtgagaatga tgaacagctt ggtggacctt tatatgataa tggcgaagtt
     6961 gtttttacag cgaaccaaaa aggaaatggc tattggaaga tcgaaaataa aaatcattac
     7021 accggttctg gatggataga agatcaggac gcacccatcc aaccaatcca agaattccct
     7081 gttgacatca cgagctacca aaccgttata gaagacaaag aaaacattac attgacccta
     7141 aaaaaacaag ttttcttacc ttatccttat ggaaatacta ccttgactga tttttcatta
     7201 gctagtggac aaacgcttta ctatttgcct gaaagccagc gattcattcc tgtcccgaat
     7261 gaatcgacac gttctgcaac tttacaaaca gaaaggttag tttttacgag agaagaatta
     7321 aaaagcagtc agttaaaagc tgatgattta gaaaatcagc agacaaatct tcaattgcca
     7381 aaaagtttgc ctaaacgtat tgaggacttg gcggtcgaat tgaccaaaaa tgaagcaact
     7441 ttaattcgta aagtcgaagc tgtctaacag tatttaaaaa caagcggaaa tttccgctat
     7501 tccaaaacgg ataccgggca tactccaaaa gaacacgatt atgtggatta tttcctcttt
     7561 gattcaaaag tcggctattg tgacaacttt tcaacttcta tggtcgtcct tcttcgaaca
     7621 cttggtatcc cagctcgctg gacaaaaggt tttacaaatg ggctcagaac aggcacagat
     7681 aatcaaggga atgaggaata cagcattctc aatagtcatg ctcattcttg gccagaggta
     7741 tactttcctg gatttggctg gcttccattt gaaccaaccc cttcatttgc caatccaatg
     7801 ggttcacaac cagttgctga aacaaccgaa accgtccagc ctgctatcga acaaccaaat
     7861 gaaagcgaaa atatggcaga aactatttcc agtgagagat ctgaggtcat tccatcagaa
     7921 gaaacaacac tcacaccagc ttataaaatc gtcttattca gccttggagt gtgtttatcc
     7981 gtcttgttgt tctttctatt gttattcaga aaatctctgc gctggacaag ttatcttatc
     8041 tatcttcatt tttttgcttc tactaatacg attgcatatc aaaaacttct taaccctttg
     8101 gaaacattgg taccgcgcaa acccgcagaa tctttgcagg aatatagcct gcgaatagaa
     8161 caagaattca agctagatct gcgtttcatt cgattaacta ctcaatatga agcagaaata
     8221 tatggacgac aagcagatcc ggtaaaattc gataaaaaaa cctatttaga aataacgcat
     8281 ataataagaa aaatggtata caaaaaaatt ctgacaaaag ggagccgctt caaaaaataa
     8341 gataaaattt ctggagaatc tacttctgga atgccgagaa ccagctgatt gagtatcgat
     8401 tactcgaaaa aaaggttgtg acacgttttt tgtcacaacc ttttttcatt attatctgat
     8461 tttcaatgtc aaagcattta gagctacaac taccgtactt aacgacatca agacagcacc
     8521 aactgctgga cttaatatga taccaattgg cgcaagaatt ccggctgcca gcggaattgc
     8581 aagaatattg taacctgccc cccaccagag gttctggatc attttctttc gcgtttgctt
     8641 ggctagatca agaaaatgca aaatatcttt tggatcgctg tttgtcaata cgacatccgc
     8701 agaatcgatt gcgatatctg tccctgcacc gattgccatc ccgattgaag cacgggctaa
     8761 acttggcgca tcattgatcc catcacccac catgattact tttttccctt ggtcaaggta
     8821 tttctggaca acagcttctt tatcatctgg taataaaccg ccataatatt cttcgatccc
     8881 taaataatct gctactgctt gggcagcgtt tttattgtca ccagtaagca tgacaggagt
     8941 gatttttctt tctttcaatg ttcggataaa ttcttttgct tctgttttta ctacgtcacc
     9001 taaagcaacg attgcagcta gatgattttc taaaatcagg aagctgatgg tatttccttg
     9061 ttcttggtaa ggtttcaata atgcttgttc tacgtttaat ccgagacgct caacttcttt
     9121 ttcattaact attttcactt gttgtttttt gactgttgcc tctagcccga ctcctgaaag
     9181 attttttaga ttttcagctt gataaggctt gatttcatgt tctttcaaat aattcataat
     9241 gccaacagcc aatggatgat tggcattcgc ttccaaggct cctagatatt gaaggatttc
     9301 atttttattg aacgcatcat caagtacttc gacacccgtt acagtaaatt ttccttctgt
     9361 tagtgttcct gttttgtcca gcatgatcac atctagatca ttggcttgct ccagtgcatt
     9421 gcggtttttc aaaagtaatc cgtttttagc tgctattgat gtggaacgag caacaactaa
     9481 tgggatagct aaacctagag catgaggaca ggcgatgata aagactgtta ccatacgttc
     9541 taatgcatta ggaagatctg tcaagaacaa ccaggcgatg aatgccagga tacccacaat
     9601 taatgcgaca taaaacagcc attttgccac tttgtcagat aatgactcta gcttggattt
     9661 ttctccttgt gcttttcgca ccatttccat aaccttcgct agataaccat tttcaccagt
     9721 tccagttact tcgatttcga tcgttccatc cccattgata gaaccgccaa tcactttgtc
     9781 attggcttgt tttttgattc ctcttgattc gccagtcaca gctgattcgt caacaattgt
     9841 ttcacctttc aagattacac catctgttgg cattttatca ccagaccgga cgatcaaacg
     9901 atcaccttcg tttacttctt tcaatgaaac tgtctcttct tttccatctt tcgtcaagcg
     9961 tttcactgat tctggcagta attctgctag tttttgtaaa gcattgctgg cattcgacac
    10021 agcattcatt tcaatccagt gacctagtag cataataacg atcaaagtag ccaattccca
    10081 gaagaaatcc atgacgtgtg tatgtggatt gagcagattt gcgataaacg aatagacact
    10141 ataaatatat gcaactgtaa tccccatcgc gatcagtgtc atcatagcag gacttttttg
    10201 tttcaattcc atcttagctc cacttaaaaa tggctgaccg ccatagataa atagtatagt
    10261 tgctaagatc aatacgagcc aatcagaacc aggaaacgtt acttgaaatg gaaattccat
    10321 tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg ataagcgata gccaaaattt
    10381 ttgtttgaaa ttccccatgt gcatggaatg atccatgccg ccgtgttcgt gatccatgtg
    10441 catcgaatgg tccatttgtt tgctgtttgc tatatctcca tggttatgtc ccatgtgcat
    10501 ggaatgatcc atctgactat ggtcatgttt catctccatc tgtttttcat gagattctgt
    10561 actttctttg tttactactt cttttttatg ctcatgtttt ttctccatat cgttcccatt
    10621 catttttact ccatcccttc tcttactttt tcatgtgtcg atttaagcac agggagttca
    10681 acaatacact gacggaacta aacgccatcg ctcctcctgc aatgattgga ttcaaaaagc
    10741 ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa agcccagaaa agattttgtt
    10801 tgatttttct caatgtcaac ctagacaact cgattgtttg tgcaatggat gccaaactgc
    10861 tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc cgttccactt cccattgcca
    10921 taccgacatc agctaatgct aaggccggtg catcattgat tccatcgcct gccataccga
    10981 cttttttgcc atctttctga agtttttcta cgtaggctgc tttatcctct ggaagtactt
    11041 cagcaaagat atgctttgga tcaataccaa cttgtttacc aatcgtttcg gctgccagtt
    11101 tgttgtctcc tgtcagcata aaaacatcta atcctttatt ctggagctgc ttgattgctt
    11161 gtttgacttc tagtttgatt tgatcagcca cggcaatcaa cccgatcact tgcttttcat
    11221 cagctaaaaa catgactgtt tttccctgct gttctaattc taacgcgtgt tcttggtatt
    11281 catcaaatga taggttcaat tcaattaatc gtttacgtgt acccgcaaaa tagcgtactc
    11341 cgttaatcgt accgctgatt ccagcacctg ggtgggctgt aaaatcaatc atatcgtatg
    11401 ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt acctagtgga tgttcagaag
    11461 cttgttctaa agtataaaaa atagatagga cttctttaga accgactaga tctgtaactt
    11521 tgggctttcc ttctgtaatc gttccagttt tatccaaaac aatgctgtct aagtgagcag
    11581 ctgcttctaa tgcttctccg cctttaatta aaattccatt tcgtgcacca acacccgttc
    11641 ctaccataat agctgttgga gtcgctaaac ctaatgcaca tggacacgca atgactaata
    11701 cagagacact atggatcaat gcgagttgcc aatcaccagt gatcaaacct gtagctatga
    11761 gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat ccctgagatc cgatctgcga
    11821 ttttttggat tggggctttg ctcccttgag catcttccac catttgaatg atttgcgcta
    11881 atactgtctg cttgccgact tgtgtaactt ccatgtgaag tagaccattc gtattgattg
    11941 tcccgccaaa gagcgtgtca tccggattct tttctacagg aagattttca ccagtaagca
    12001 tactttcatc tatcgcactg cttcctgaaa taattcgacc atctgctgga atctgctcac
    12061 caggacggat gactagttga tcaccaacga ctacttcttc aatcgctaac gtttcttctt
    12121 tcccgtttct tatcacttgg gcggtttttg tttgaagaga catcaattgt ttgattgcat
    12181 tacctgtttt cgtttttgct gtatgttcca aatatttgcc gagcaaaatc agcgtgatga
    12241 tcatactgct gctttcaaaa taaagatctt gggggtgtcc acggaaaaat ccgttgtaaa
    12301 tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa tacatccata tttggtgctt
    12361 tcgtcttgat tgcatggtac gcacctttat aaaaacgagc accgacataa aattggacag
    12421 gagcagacag cactaactgg acgattggaa aatgaaaaaa gtgaacaatc gccgcatggc
    12481 ttcctaacat catagcaatc attgacaaca tcaacggcaa agtcaaaacc gtactcaata
    12541 taagatcgaa tagcattttt ttcaaatacg cttgcttttc ttctgcaatt ttttgtttgt
    12601 gggcttcatc gtacaaaatt gcaccatagc cgatgttttc tacgctttgg attaattttt
    12661 cagtggtcgt cccttcatat ttgacacttg ctttttcagt ggccaagttg actgtggcat
    12721 tgaccacacc aggttgtccg ttcagctctt tttcgatcct agcagaacaa tttgcacaag
    12781 tcatacctgt aatgacgaac gtatccattt tggtttctgc tgttgccatc aaatcacctc
    12841 tgctttataa cccaatccat tgattgcttg acaaatcttt tcggattgaa cttttgtttc
    12901 atcaaacttg accgtaccgt ttgctttttt caaatttact ttcacttttt gcacgccgtc
    12961 aagtgcgctt actgtttctt ctactcttgc tgcacaatga ttgcagctca taccttcgat
    13021 tgaaaattgt tgtttcattt tttcttcctc ctattgatgt tgtttacatt cacattgacc
    13081 tgggatacag ttacattcga tcgtatctac tggttgcttt tgttccagtt gtttcaaaat
    13141 ctcattgata tcttcttgag tcaaagtggc ttcttctact agatccgcaa tcgtctctcc
    13201 aatccttttt gcacagatat gggaaaaaag attttccgtc gcactgcgaa ctgtttccat
    13261 ctccgagaca gctggataat aaatgaattt ttttccttct tgttctgtcc agatcacttc
    13321 tttttttacc aaacgaccca gcaaagtttt gatcgttgct ggtttccaat ccatcgaatc
    13381 agctaagatc tgcgtgattt cttgggctgt cgtctgtcca agtgtccaaa taacacgcat
    13441 gatctcccac tctgaatcac tgatctttac tggcgttttg actttttcca tttatttcac
    13501 acctttcgtt tacaaatgta atcattatct tatgaataca gattacactt gtaaacaaaa
    13561 actgtcaatt atttttgctc tctattttca atttagttaa aaattcttgt ttctctcgtc
    13621 ttgttttaag gatgatcagt ttatttcttg cattagctac ttggatcagc tgctgtattt
    13681 tcggttcatt gttttcttca aaatgccaga taaaagataa aaattctaag tattccctgt
    13741 ttagtttctc cgaaaatgat tcaggcatat ctggccgaga ttttttgttt atctttcttt
    13801 ttagagaacg ccaaactact cggtagatgg cgagataacg cggcacttta agccaaatga
    13861 tcttatctgc ttctttaatc ctttcctcta atgtagaagt ataatttcct tcaacgatcc
    13921 atgacggatt agataccata aacaattgct gttcttgcaa aaaccacttt tttgcctgaa
    13981 tactgtagtc tgttgtatgc cataaaaggt ctagcggcag tagcggaaag tcggtgattt
    14041 ctttgatctt ccttgcgagg gtcgattttc ccgacccact ttgtccaatc acgatgatct
    14101 tcattcttct ccctcacttc tctttggaat ttggttcgat cataccactt ataacgccgc
    14161 aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc cttgtttcga ataa
//
LOCUS       DACEOM010000076        14176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEOM010000076 DACEOM010000000
VERSION     DACEOM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.079"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(221..1177)
                     /locus_tag="IW781_002357"
     CDS             complement(221..1177)
                     /locus_tag="IW781_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1183342.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IW781_002358"
     CDS             1281..1883
                     /locus_tag="IW781_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1183343.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IW781_002359"
     CDS             complement(1971..3470)
                     /locus_tag="IW781_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1183344.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IW781_002360"
     CDS             complement(3486..4010)
                     /locus_tag="IW781_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183345.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IW781_002361"
     CDS             4308..5057
                     /locus_tag="IW781_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1183346.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IW781_002362"
     CDS             5048..5833
                     /locus_tag="IW781_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1183347.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IW781_002363"
     CDS             5802..6518
                     /locus_tag="IW781_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1183348.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IW781_002364"
     CDS             complement(6620..7639)
                     /locus_tag="IW781_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1183349.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IW781_002365"
     CDS             complement(7658..8716)
                     /locus_tag="IW781_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1183350.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IW781_002366"
     CDS             complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IW781_002366"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1183351.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10161..10997)
                     /locus_tag="IW781_002367"
     CDS             complement(10161..10997)
                     /locus_tag="IW781_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183352.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11181..12398)
                     /locus_tag="IW781_002368"
     CDS             complement(11181..12398)
                     /locus_tag="IW781_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1183353.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12781..12864
                     /locus_tag="IW781_002369"
     tRNA            12781..12864
                     /locus_tag="IW781_002369"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12815..12817,aa:Leu,seq:gag)
     gene            complement(13104..14102)
                     /locus_tag="IW781_002370"
     CDS             complement(13104..14102)
                     /locus_tag="IW781_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183354.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4320 a         2872 c         2427 g         4557 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
     8881 aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat
     8941 gcgtggaaac gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat
     9001 acgataaagt ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag
     9061 ctgattgctg gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag
     9121 tctacaacat ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat
     9181 tctagacgtg ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca
     9241 tcaggcatcc caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct
     9301 ttttccatta ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca
     9361 gaaaagacat gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat
     9421 ccacgcatac cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc
     9481 gcttcttcta gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact
     9541 tgtttgacaa actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa
     9601 ccacctgaag cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt
     9661 gggttttgtg cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg
     9721 attttagctc ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa
     9781 cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga
     9841 ccttcagaca tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag
     9901 ccgtgaatat gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg
     9961 ttgacttctc cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca
    10021 agatagcctg catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt
    10081 ctcattctat tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag
    10141 tcaagaatgg tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag
    10201 ccttcataga atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc
    10261 acaaataaat cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga
    10321 ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta
    10381 taaaaaattt gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg
    10441 atgcttcgac tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt
    10501 ccgatacaac taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta
    10561 gagtagctta atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg
    10621 aattcgcaaa caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt
    10681 tcagaaatat cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa
    10741 caactatcca acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc
    10801 atcacatgat caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc
    10861 ggcagttcct cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa
    10921 agcatttttt gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac
    10981 tcgattaacg tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat
    11041 tttactttag gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt
    11101 tatttaagaa aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact
    11161 gaaaaatttg gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc
    11221 ttagctgttt gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc
    11281 tggttccaat taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc
    11341 aaaacgaatt tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata
    11401 taaaaagcag cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag
    11461 atatattctc tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga
    11521 taaccgccta gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg
    11581 gaggacaaca tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg
    11641 accatccagc ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta
    11701 acaacgaaca aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact
    11761 aagcgatcat agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg
    11821 atttcttcta ataattcttt tggataaagt gccccagtag gattattagg attaatgata
    11881 acaatagctt ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat
    11941 tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc
    12001 gtccacaacg gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc
    12061 tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc
    12121 acattcggaa aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc
    12181 cccttggaat ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact
    12241 tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta
    12301 atgccctctt cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg
    12361 acaccttcta atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc
    12421 aaaaagttag aactttccaa attttttgtt atttactatt tcaatatcat actccatcct
    12481 agaaaatttt gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt
    12541 gagaacttaa ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc
    12601 acaaatgatt cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac
    12661 acctaagatc attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc
    12721 tcccattttt gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac
    12781 gcagatgtgt tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg
    12841 tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt
    12901 ttttgtttga acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga
    12961 acgtacaatc atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt
    13021 agaaacttgc aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac
    13081 taaagcgagg gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata
    13141 ccaagcacgg ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt
    13201 agctctttaa ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc
    13261 cgcattccgg aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc
    13321 attatcatca aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt
    13381 ccaacaatta aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga
    13441 taaaaaagtg acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc
    13501 caaatcattg cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca
    13561 aaataaataa tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt
    13621 tgacatttcc tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc
    13681 atgtctaaaa aaaacggctt tccaaatgat gaaataccta aatctaactt attaagaaag
    13741 ttgaaatttt tatattcagg aaaccattta ttaatttcat gtgcagcacg taatgcattc
    13801 cctggagttg tcaaagagaa aattaattct gctaagctta ttattgaaaa tactgataac
    13861 ttataattat atcgtccatt gacaatcaaa tataaactaa ctatacttgt tagaacaaaa
    13921 aaacaggcgt tgacttgttc ttgattagtt aaaaatatca ataaaggaat acttacccaa
    13981 tatatttttc tatttatttc ttctcctctt aagagttgaa aaaacggata aaaagttagt
    14041 aaactaaatg ctactggcca ctgatagttc aaggtggttg ctctccaacc ggtttccccc
    14101 attactgtca atggtattaa acagtatata gagcaaacca ataataaatt tttaacattt
    14161 attttctcaa aaacat
//
LOCUS       DACEOM010000077        13919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEOM010000077 DACEOM010000000
VERSION     DACEOM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.080"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(51..707)
                     /locus_tag="IW781_002371"
     CDS             complement(51..707)
                     /locus_tag="IW781_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1183355.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            900..1178
                     /locus_tag="IW781_002372"
     CDS             900..1178
                     /locus_tag="IW781_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183356.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1197..2405
                     /locus_tag="IW781_002373"
     CDS             1197..2405
                     /locus_tag="IW781_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1183357.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2553..2918)
                     /locus_tag="IW781_002374"
     CDS             complement(2553..2918)
                     /locus_tag="IW781_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1183358.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3065..3838)
                     /locus_tag="IW781_002375"
     CDS             complement(3065..3838)
                     /locus_tag="IW781_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1183359.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4012..4872)
                     /locus_tag="IW781_002376"
     CDS             complement(4012..4872)
                     /locus_tag="IW781_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1183360.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4872..6290)
                     /locus_tag="IW781_002377"
     CDS             complement(4872..6290)
                     /locus_tag="IW781_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1183361.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6347..6796)
                     /locus_tag="IW781_002378"
     CDS             complement(6347..6796)
                     /locus_tag="IW781_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1183362.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7241..7504)
                     /locus_tag="IW781_002379"
     CDS             complement(7241..7504)
                     /locus_tag="IW781_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1183363.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7603..8898)
                     /locus_tag="IW781_002380"
     CDS             complement(7603..8898)
                     /locus_tag="IW781_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1183364.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8895..10166)
                     /locus_tag="IW781_002381"
     CDS             complement(8895..10166)
                     /locus_tag="IW781_002381"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1183365.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10331..11200)
                     /locus_tag="IW781_002382"
     CDS             complement(10331..11200)
                     /locus_tag="IW781_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1183366.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11492..13102)
                     /locus_tag="IW781_002383"
     CDS             complement(11492..13102)
                     /locus_tag="IW781_002383"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1183367.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3960 a         2933 c         2374 g         4652 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctttgttca tcaaaagata
       61 atattgtctt ctaacaagtc catattcaaa ataacgatat gatggtcttt aacggtgata
      121 ataccatctt ttttcagttg ttttaaaatt ctgtttacac tgctagcagt tgagatgcca
      181 caaaatttgg cgatttcttc attcgtgatc gaaaaatcga tcagcatccc ttcttctgtc
      241 cgcacaccga ataaatcata taattcgtaa agttgcgtac aaacagctcc cagctttccg
      301 tttaatagca tttgctgcat ttttttcatc gaataaagca gacgatagcg gtaatactct
      361 tttacatagt gttgtagctt tgggtcttgg ttgatatttt gccaaaagcg cacacgattg
      421 attcgataaa actctgcata ttctgattca actcgaatat tatacggcga atcgatgaat
      481 tgtgaatatt cgtcttttaa aagagagacg atttcaagat ctttgatata gcgaaggtta
      541 aattctctcc catcatttga aataacactt gcttttaccg ttccctcttt caagatatac
      601 gtatgggaat cttccatccc ttcgaatgtc aaatatttat gatatccttt gcgtataatc
      661 ggaaaatcat ttttatccaa atattccttt aagactctac tatccaatga tgctccccca
      721 tccaaacttt catctaaaaa gatcatacag tactttaaaa ctttttaaag tccaaaggta
      781 ttttactaat gatattaaca tttgttaagt acctttttcc atcacttatg cgattttttt
      841 cctttttttt attcgttata ctgcagaagt aaaatttttt aagaatagag ggaaaaaaca
      901 tgaattactg gatgaacaca atcattaatc gattagaaac agcatatcaa acaagatttg
      961 atatgaaagc ttctttagtc tttttaaatg atgcgtatca aaactcgatc gaattgatca
     1021 aagcagtgga tgagaatcca acaaacgaat gcgaagaatt tttaaatctg tttatgtcta
     1081 caagagattt atttattcgc caacttgttg atcgctatcc atccaattat cacgatgtag
     1141 aagttcaaat acaaaaatta aaagcatatt cagcttaagg ggaatgtata acatacatgg
     1201 aaaagaaatt atttaataaa tattttattg gaatcacatt gatcaatttt attgtctacc
     1261 tgatttatta tctgttgatg gtcatcatgg cagtcattgc gcaagacacc ttacacgcca
     1321 gcttgagcca agcaggtttt gcttctggga tctatatcat cggaacattg ctggcacgct
     1381 tattcatggg gaaacagtta gaactatttg gtcggaagtt taccttaaga ggcggcgcga
     1441 tcttctatct cctttcaaca ttagcatatc tttatacgcc aactatcggg gcattgtatt
     1501 tagttcgatt cttgaatgga ttcggctacg gtacagtttc tacagctaca aatgctatcg
     1561 ttactgcata tatccctgca tctaaaaaag gggaaggcat caattattat ggattaagta
     1621 ctagtttagc tgccgctgtt gggccatttt taggaatgat tttgctgaat atgactgatt
     1681 ttcgtttcat tatctggttt tcaattgtac tggtcttctt tgttacgatt gcctgccttg
     1741 tctttcctgt aaaaaatatt ccattatctg aagaacaaaa aatatctctt cgctcatgga
     1801 atatcaatag ttttattgaa aagaaagcac ttttcattac aggaattgcc tttttgatgg
     1861 gtctttctta ttcaagtgtt ttatcatttt tatcttctta tacaaaagta atccacttag
     1921 ttgctgctgg gtcatttttc ttcgtcgtat atgctttggt catcacattt acacgtccat
     1981 tgactggaag gattttcgat gtcaaaggag aacaatacgt gatgtatcct agctatttat
     2041 tcttaacagc aggattattt ttactgagtg taacgacgaa tagtgtgaca ttgcttatct
     2101 ctggagcatt agtcggccta ggttatggta cgtttatgtc aaatggacag gctgtttgtt
     2161 taaaaatcgt ccgcgaacat cgaatcagta ttgctttatc tacctacttt attggtttag
     2221 acctaggctt aggcgttggt ccttatttga tggggatgct aaaatctctt acttcattta
     2281 gaggattgta tgtgattgcc ggagtcattc cattgatatg tacagcactc tatcttgtta
     2341 atggccgaaa acatagtcat gaagcagagg gcgaattgat tgaatcattg aaaaatgaaa
     2401 tttgatcttg atgaatagat aaaaaaaacc gttcagatct gaaaataatc tgagcggttt
     2461 tttgagtaaa aatagtttat gattattctt aattaaacat ctatttattt ttaaagcttt
     2521 catttatcga taagaatagt ttaactgttg atttattcca ttttccgttc atctggtacg
     2581 atgccttgac ttagcaacca cttgtggaag aaccattctg ccacaccgat caatagagca
     2641 ggaatcaata ctttcatcca aatatcgact gtatcattca taaaatatag atagagccac
     2701 aagataactg cagctgaacc agcatctgcg atcgtcgctg cgatgttccc tactttacgt
     2761 aagatcaaca gatcccctag cacatagatc atgactacaa taattacccc aacaatcgtt
     2821 gaatccataa agctaacacc gtataaacct gtcagcacga cccacagaat cgcccagatc
     2881 atgattgcct tcactaccaa cgccttcaca tgtttcattt tctgcactcc tttctcctgg
     2941 ataaaaaagt aagattatct aacaagataa ccttttcatt attataccat cggctgagag
     3001 aagcgagcaa agataaggtc ctatctaaaa atgaggaagc cacttagaac attcacgtaa
     3061 taatttatcc gtacagacat tcgatatggt gttcctttac tttcgcatac tgttcttttg
     3121 tcagttcttt acttgtcacc agataatcga tttcatgaaa agcaatggct tgatgcatga
     3181 atttatgtgc aattttttgc tgtgtaacta aaacggcaag ccggtctgat tgttttctaa
     3241 aagctttttt gaccattgcc tctccttcat gagtttctgt tactccttgg ctgaaatcta
     3301 gcccgcgaca actcattatc gctaaatccg cataatatga caatgcatcg ttataggctt
     3361 ttgaaccgtt gattgttgct cgtttcggaa caattgcccc tcccaataca gatacttttg
     3421 ccgttgtata ttcactcaac atagcagctg tgacgatgcc attcgtaatg atctgcaaac
     3481 catcaaatct tgctaggttt ctgactaagt gtaaagaggt tgtactggaa tccaagaata
     3541 agaccatatc atctcgaatc aaagaagaag ctgtatctgc aatcttacgt ttttctgttt
     3601 cttcttctaa tttggtgatc ttcatcaggt aaggcacgtc aatttttcga ttgtcaataa
     3661 gcatggcgcc accgcgaact ctacggatca atccactttt ttccagaaaa acaaggtcac
     3721 gtttaatcgt ggcttcgcta taatttacta actcgatcaa atcggtgact cttgcaaatt
     3781 ggttgttttt tagataattc acaatctcct gctgtctatt ctccacttca ttcaacataa
     3841 aatcacccca tttactttat atagtaacag gaaaaaagag gttgtgacag aagtgattgg
     3901 cttcgagaaa taagaagaaa tttttcgaaa aaactacttc tgaacatcgt gtgtggttaa
     3961 gagggtatga caagactatt gtcataccct cttgaccaca catttttatt tttagtataa
     4021 actagccttg cctactgtgt tcaaaatttc cattttatgt ttgaccactt cttgtacctt
     4081 tttattagct gctggataaa ttgcattcgg ctcgtacatc gatggatttt cactcaatat
     4141 ttctctcaaa gcattgaaaa agacgctttt aagatctgag ctcaaattca ctttggcaac
     4201 tccgtatttt actgcttcgc tgacttctgc gtccggatta cctgaaccgc catgtaaaac
     4261 aaatggaaga tcgatttgtt ggttcaattc tttcagtaag tcgatattta attttggagt
     4321 tctgtttttc ggatacaagc catgcgctgt tccaattgct acagcaagtg tatcgattcc
     4381 tgtttctttc acaaaaatct tcgcttgatc aggatctgta taaatgattg tttcagcacc
     4441 tccttcagca gaaccattat tcccaatcgt tccaagctca gcctccactg agacatttac
     4501 cgcatgagct aacgcaacca cttttttcgt aatctgtaca ttgtcgtcga atgatttcaa
     4561 agacgcatcg atcatcacgg aagaatagcc gtttcgaatc gcccgtaaga catcttctat
     4621 cgtaccgcca tgatccaagt ggataacgat cggcagttta cttgtataag cataggctct
     4681 tactgccgca acaaactcat cacttaaata ggcgatttca tcaggatgaa tttctaagat
     4741 tactggtgct tctaatctct ctgcttcttc gatgattgct ttcatcatat catagctgca
     4801 aatattgaag gctggtactg caaattgatt ctctttagcc actttcaata actctttcat
     4861 tgtatataac attacgcttc cacctcattt gttagatttt catttttcgt ccacttgtct
     4921 tgttttttct ctttcactgt ttttttcaat agtccaagca taaaggctgt agtgactgta
     4981 cctgatgcaa ttgccaagac atacataggt aaattgttca taacatttgg aattagcatc
     5041 acccaaatcc ctccatgagg tgccattgaa gtaactccta ctgccatgga aatccctgca
     5101 gcgactgctg aacctgtcat caaactaggt atcacgcgga tcgggtcaga taccgcaaac
     5161 ggtatagcac cttctgtaat atacgaagcg cctaaaatcc agcatgattg acctgaagtc
     5221 cgttcttctt tggtaaattt atgtttgaat aataaagtgg ctaaagcaat acctagggga
     5281 ggtgtcattc ctgcagccat acaagcagca atcggtgcat ttactccagc tgacatcaaa
     5341 ccgatcgaga aagtcgaagt cgatttattg atcggcccac ccatatcagc tgccatcatg
     5401 atcccgataa gtaatccgaa caatagcccg gatgtatttc ccaagctatt cagccaattt
     5461 gtcaaagcat ccaacaccca tgtgatcggt tgttcaatca caaataccat caaaagccca
     5521 ataattaaga tcgataaaaa ggggacgaca atcagctgat aaatagcaga aaattctttt
     5581 tttacgctaa ttttatttgc cagaaaatca cagatatacc ctgctagcac gccaccaatc
     5641 attccgccaa gaaaacctga accacctgta gaggccagta atccggcaac aattcctggg
     5701 gcaaaacctg cttttccagc aatcgatgta gcaatccccg cgcctaacgc aggaaccatc
     5761 atggcaaacg ctgtatctcc tccgatcctt gaaaatgcag ctgctaacac attatagtct
     5821 gctgagttgg gatcagccgc tgtaatccca aaggcaaaac tcagtgcgat gatgattccg
     5881 cctgcgatca caaatggaag catgtaattc acaccattca tgaaatgatt atagattgtc
     5941 ggctttttct catttgcagc tggttctttt tcattagaca caacgaatac gccaatatct
     6001 ctcaatgctt cgtctattac tacttgcggt tctttgattg ctcggcttgt agagacacgc
     6061 tttacttttt tcccggcaaa tcggtccaat tcgatttctt tatctactgc aagaatgatt
     6121 gcatctgcct ctttgatttc ttgttcagat aactgatttt ctgtttttac accttgtgtt
     6181 tcgaattttg cttgatggtt tagatttctt gctgttactt ctaatttttc tgccgacata
     6241 taagtatgag caattccagt cgggcaagca gttacaccaa cgattttcat gattattttc
     6301 ctcctttttc ttcactatcg atactcggac aaagactcta ctattttcat tcttgactaa
     6361 tctgtctgat gatatctgct ggcgtagtgg cttcttttat tccctttcta aaatctgcat
     6421 tcatcaatcg tctggagagt gtgctgagaa tctctagatg catatctgct gatttttcag
     6481 gaactacgat ggcaaaaata tattcaattg gcatttcatc tatcccgcta tagtccgtta
     6541 atttggctgt atgaaaaaaa cacagtgctg gttttgtcac atatttgctt tttgcatggg
     6601 gaatcccaaa tccatcttct attcctgtgg agatctcctc ctctcgtttg aatagtgcct
     6661 taacgacttt ttgaacattt cttaccttat ttgttttttt caacacatcc cctattcgct
     6721 caaataatga tccttttgaa tctatcgatg gatcgaaaac aatcagttct tctgcgatca
     6781 attgatcaaa cctcataaaa aacctctccc ccacaaaaaa tgttaacgtt ttcttggaac
     6841 tgaggtcagt atagcatcgc tagctgtacc gttcaatatt atgaatgaac agaggaaaaa
     6901 gacccatcat ccgcgtgtat aagtcattaa aatgaacgat ctagctcaca aacaatcgca
     6961 agtctataac aaaacaaaaa gccttccttg aaaggaaagc ttagcttcta attttaggta
     7021 aatggttttt tacttcgaaa taacccctct tcatttcgtt aatataaggt gtttttatca
     7081 agtggataat taaaactata tactagtttt tcattgtttt gtgcattatg atcctacgac
     7141 tttttcagca tcaaacgaag ttttaaattg gcaaaaaaca taaaaaaaca gactatccct
     7201 atcagatagt ctgctaaacg ttgagttatc agcgttttta ttattccgca gcgtttgcgt
     7261 ctttgagacc gtattttttg ttgaaacggt ccacacgtcc gtctgcttgt gtgaattttt
     7321 gacgtccagt gtagaatgga tgtgagtcag aagttacttc gacacggatg actgggtatg
     7381 tattaccgtc ttcccattca actgtttctt gtgaagtttt tgttgaacct gacaagaatt
     7441 tgaaaccagt tgttgaatcc atgaatacta ctgggtggta atctggatgg atgttttctt
     7501 tcatgatttt ttcgctcctt tgccctgctc catttgctga accagagttg atttgtcgat
     7561 ttacaacatt aacatcttaa catgtttcag tcaaggttgc aattatcttt tcagatttct
     7621 ggtcggattt tttttaccaa aggagacatc ttgaaattct ttgaaaaatt gctgattgtt
     7681 ttttgtttta cgcaaaaagc ggatcagttg ttccgtatat tctaatgaat ctccagtcat
     7741 attttttcga agtttccaaa tttcttctaa ttcttcactt ggcatcaata attcttcttt
     7801 tcgggtacct gattttttga tatcgatggc tgggaagatc cgacgttcag ataattcacg
     7861 tgaaagttgt aattctagat ttccagttcc tttgaattct tcatagatca cgtcatccat
     7921 acggcttcct gtatcgacta aagcggtcgc taaaatcgtc aaactgccac cttcttcgat
     7981 gtttcttgct gccccgaaaa atttcttagg tttatacaat gcggctgggt caatcccgcc
     8041 actcaatgta cgtccgcttg gtggaacgac taagttatag gcccgagcaa gtctagtaat
     8101 actatccatc aagataacga catctcgttt atcttcgact aaacgcattg cgcgttccaa
     8161 tactaattca gaaactcgag tatggttttg cggctgcaga tcaaatgtag aagataccac
     8221 gtcgcctttc acgcttcgtt ctagatccgt tacttcttcc ggtcgttcat caatcagtag
     8281 aacgattaat tctacttccg gatagttttc ggtgatacca tttgcgattt cttttaatac
     8341 agaagttttc cctgcttttg gcggtgcgac gatcaatcca cgctgaccaa aaccgattgg
     8401 agaaaagata tcgatcattc gggtagatag tctttgcggt gttgtttcca gttgaagctg
     8461 tctttgcgga taaagaggtg ttaaggctgg aaaatgcgga cgttcttttg cttcttccgg
     8521 atcttttcca ttaacgcttt ccacgtgcat caaaccataa taacgttccg attctttagg
     8581 tggacgggct tttcctgcca ctttgtcccc atttctcaaa ctgaatcgtc tgatttgtga
     8641 ggcagaaata tagatatctt ctgcacttgg tccataattg attggccgta aaaaaccgta
     8701 accatcttga ccaacgatat ccaatattcc ttccatataa taaaatcctt gtttctctgc
     8761 ttgtgcacga atcacagcta gagataattc ctttttattc atcttactgt aataaggaat
     8821 tttaaattct ttggcataac tataaacttc ttttaacgtt ttgttctcta gttctgccat
     8881 tgtcaaataa tcgctcattc agctcccact tcctctatca tttcaattga tgcccctaag
     8941 gctgttagtt tctcgataat atgatcataa ccgcgtaaga tataatctac attgtaaatc
     9001 gtcgttgtcc cttctgccaa tagtccagca atcactaaac aagcacctgc ccgaagatca
     9061 gaagccacga cttctgtgcc gtgaagttta tttggaccat tcacgatgat catatttcct
     9121 tccacggtaa tatcagcgcc cattcgtgct agttccggca catggttcac acgtttcgca
     9181 tagatcgtat cgatgatttc tgaagtgcct gtagtcatca atagtaacgc agtaagtggc
     9241 tgctgcagat ctgtggcaaa tcctggataa ggataggtca tgatgtttgc gggttttaat
     9301 tctttcgagg ggtaaacttc gatttcatct tcgctgattt tcatatggac accaatttcc
     9361 tgcaattttg ctataaagct ttccaaatgt tcaaagatca catttttcac tttgatacca
     9421 tttcctactg cagctgctaa tgctagatag gttcctgctt cgatgcggtc aggaatcatg
     9481 aaatgccgac agccatgtaa tgtttcgact ccttcgatac ggatcacatc tgtacccgct
     9541 cctcgaactt ttgcccccat attattcagt aaagttgcca catctatgat ttccggttct
     9601 cgtgcagcgt tttcaattac tgtctgtcct tttgctttta cagcagctaa catcacattg
     9661 atcgttgctc cgactgaaac cacgtccatg aaaatccgat tgccacgcaa tcctttattt
     9721 tctgtccgta aatacatggc accatgttca tttgtcactt cagcacctag agcttcaaat
     9781 cctttgacgt gtaggtcaat cggtctaggg cctaaataac agccacctgg caggcctact
     9841 accgcttcgc caaatttacc aagcagtgtt cccataaaat aataagaagc acgcaaacta
     9901 ttgatttttc cgtgcggcat cggaatcgat acgacttctc gaggatcgat ttctagtatg
     9961 ttgttggaaa aatggacagt tgcgcccatg atctctaaaa tttcaattaa tgaatgaaca
    10021 tcttgtatgt ccggcacacc ttccaagatc actggggaat cagctaagat tgcagctgga
    10081 atcaatgcga ctgcactatt ttttgcaccg ctgatcgtta cttcaccagc taaaggtcgg
    10141 tttcctttga ttacgatttt tttcatctgt ttccaccttg tttattaatc ttttttcatg
    10201 gatatgcttg tattttatca tagataaaaa aaagaaacga gcatggtctg ccgttttaag
    10261 taaaaaagaa tggagagcga cttcaaaagt cgttctccat tcctatatat cgtatctgtt
    10321 atgtggatgc ttacgctttg ttagctgaac cgaaccagtc gatatgagat ttagcatctt
    10381 caacgattgc tgctttacct ggagctaata atttacgagg gtcgaatcct ttaccttctt
    10441 tatctttacc agcttcgata tatttacgag tagctgcagc aaatgataat tggaattcag
    10501 tgttaacatt tacttttgag atacccattg agatagcttt ttcgatttga tcttgaggaa
    10561 tacctgatcc gccatgtaat actaaaggca tgtctggacc tacagcatca gcaattgctt
    10621 gtaagtggtc aaaagctaat cctttccagt tttctggata ttgaccatgg atgttaccga
    10681 taccgcaagc taagtagtcg atacctgtag caaccatttg tttgcattct tcaatatcag
    10741 ctaattcgcc gttaccgatg attccgtctt cttctccgcc gattgaacca acttcacatt
    10801 caactgagat accttttgca tgtgcttttt caacaacgtc tttagctaat ttcaagtttt
    10861 cttcaaatgg tagatgtgaa ccatcgaaca ttactgaagt atagcctaat tcgatacatt
    10921 ccaaagcagc ttcgtaatca ccatggtcta agtggatagc aactggaaca gtgatgttca
    10981 ttgcttcaac taaatcagtg attaaatctt tacagatttt gtaaccaccc atgtatttag
    11041 ctgcacccat tgatgtttgg ataagtactg gtgcttgttt tgcttcagcc gcttcaagga
    11101 ttgcttgagt ccattccaag ttgtttgtgt tgaatccgcc gacagcgtaa ccgcctttac
    11161 gagcagcttt taaaaattca gctcctgata ctactggcat aataaaattc ctcctaagtt
    11221 ttgtaaaact aaatttgtta agacaattct taaccaacga ccctatttta ccaaacattt
    11281 cggtagtttt ctactaaaat caatgaaaat gaaaattttc aagaagtttt ttgcaaaaaa
    11341 taaaatatcc aatagcgctt taatcgaaaa aagtcttttg ctttctatct tttttcctac
    11401 aaataataac aaatcagaaa aagagaaaag tgacaagaaa attgcttatc cccttctctt
    11461 gctacttttc tctttctatc aagttcaaaa attattttgt ttcgtgatct gctaatgatg
    11521 ctccgacaaa tgctttgatt aatttttgcg ggcgatttgg acgagaaatc aattctgggt
    11581 ggaattggca tcccacaaag aattttttct ctggcagctc tacgatttct actaaacggt
    11641 tatctggtga gacacctgaa aagactagcc cttgttcttc aaacaattga cgatatttgt
    11701 tattgaactc atagcggtga cggtgacgtt cttgcacaac atcggctcca tcatatgctt
    11761 cagccgtctt cgttcctttt ttgattttac atgggtataa tccaaggcgt aaagttccac
    11821 ccaaattctc gatgttttcc tgatcagcca ttaaatcaat aatattgttt gctgtatctg
    11881 gattcgtttc agctgatgac gcatcttcta atccaacgac gttacgtgca aattcgacac
    11941 aagccatctg cataccaaga caaataccta aaaacggaac gtcattttcg cgagcataac
    12001 gaatcgcttc gattttccct tctactccgc gatcaccaaa tccaccagga accaagattc
    12061 catctgcgtc tttcaaacgt tcttctacat tttctctcgt taattcttgt gctttgatcc
    12121 aatcgatctc gatgtctgaa ttataggcga aaccagaatg tttcaatgct tcaactactg
    12181 agatatatgc atctggtaac tcgacatatt taccgacaag cgcaatccgt acttttttct
    12241 tcaagttcaa gacacgttct tctaattctt tccattctgt catgtctgct tctggtgtat
    12301 ctagttttaa atggtcgcaa acaatttgat ccattccttg tgcttgtaaa actaatggga
    12361 tggagtaaag tgtatctaca tctggcgact cgataactgc ttctggttcg acatcacaaa
    12421 attgtgccaa tttgtttttc acgctttgag aaacaggttg ctctgtacgt accaccaaaa
    12481 tattcggttg gatacctaaa ccacgtagct cttttacact gtgctgcgtt ggtttagttt
    12541 tcatttctcc agcagctttc aagtaaggaa tcaatgttgt atggatatac ataacattat
    12601 ctgtaccgac atctgctttc atttgacgaa gtgcttcaag gaatggcaaa gattctatat
    12661 cccctactgt tccccctact tctgtaatga tcacatctga atcagtcatt ttagctgctc
    12721 gcatgatttt ttctttgatc tcatttgtaa tatgaggaat aacttgaaca gttgctccca
    12781 agtattcgcc cttacgttct ttacgcaaaa cttcagaata gatttttcct gtcgtcacgt
    12841 tggagtattt gttcaaattg atgtcaataa aacgttcgta gtggcccaga tccaaatctg
    12901 tttcagcacc atcatctgtt acgaatactt ctccgtgctg atatgggctc atcgtccccg
    12961 gatccacgtt gatgtatgga tcaaattttt ggatagttac ttttaagccg cggtttttca
    13021 ataagcgtcc taaagaagct gcaacaatcc cttttccgat tgatgaaacg acaccgccgg
    13081 tcacaaaaat atattttgtc ataagttcaa aaactccttt ttaatttatg tgcaggaaaa
    13141 gtctcagaca ataaacaaag ctccctattc ttcaataaga atagggagcc gaaatttctg
    13201 taaacttctg accctctaaa agggtgccca aggtgtataa tacaagctga ctaagggaaa
    13261 gtcaagtact caaatgaata ctatccgctt acaatttttt ctttttcgat atcctttgag
    13321 aaattatttc ttaagtcctt tatgagtgca aaaaaaattt aacccacata ataagcttga
    13381 tataaacata gctaacgtta aacctaagca aatcagaaaa tcgttactga aaaattttaa
    13441 gtgaaataat agataaaaaa ataaaacaaa cattatgata gataatatcc cacctaacaa
    13501 agaactactt tttcctactt gcagcaacag tttatatagc ttatcgtaga aacaatcttt
    13561 ttctttcacc cttatttacc tcctttacgt aaaaaacgta gtccacagtt ctcggatttt
    13621 tggcatatct tttctattaa ataatatcaa aaacaaaaca attccactaa ttttttcaaa
    13681 gatgaccttt ttttcaacaa aagtgccttg tagggattca ttgaaggtaa cgaatcacat
    13741 aagtaaaaaa taaggagatg cgacaaaagt cgtgcctcct tatttctaaa taaacggtgt
    13801 tctcctgcca actcctcggt cacaagtcac aatatccatc aaacatgaga agctgttttt
    13861 gaaattattt cttgtgattc ggagttaaat ggtaggctca caacctctgg ataaacggt
//
LOCUS       DACEOM010000078        13917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEOM010000078 DACEOM010000000
VERSION     DACEOM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.081"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..505
                     /locus_tag="IW781_002384"
     CDS             131..505
                     /locus_tag="IW781_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1183368.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IW781_002385"
     CDS             complement(619..1248)
                     /locus_tag="IW781_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1183369.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IW781_002386"
     tRNA            complement(1382..1454)
                     /locus_tag="IW781_002386"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IW781_002387"
     tRNA            1668..1741
                     /locus_tag="IW781_002387"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IW781_002388"
     CDS             1696..1845
                     /locus_tag="IW781_002388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183370.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IW781_002389"
     CDS             2338..3666
                     /locus_tag="IW781_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1183371.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IW781_002390"
     CDS             3868..4701
                     /locus_tag="IW781_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1183372.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IW781_002391"
     CDS             4717..5454
                     /locus_tag="IW781_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1183373.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IW781_002392"
     CDS             complement(5609..6577)
                     /locus_tag="IW781_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1183374.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IW781_002393"
     CDS             6809..7642
                     /locus_tag="IW781_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1183375.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IW781_002394"
     CDS             7676..8515
                     /locus_tag="IW781_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1183376.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IW781_002395"
     CDS             8541..9101
                     /locus_tag="IW781_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1183377.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IW781_002396"
     CDS             9300..11021
                     /locus_tag="IW781_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1183378.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IW781_002397"
     CDS             11186..12328
                     /locus_tag="IW781_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1183379.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IW781_002398"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IW781_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1183380.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACEOM010000079        13885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEOM010000079 DACEOM010000000
VERSION     DACEOM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.082"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            307..651
                     /locus_tag="IW781_002399"
     CDS             307..651
                     /locus_tag="IW781_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1183381.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            652..1071
                     /locus_tag="IW781_002400"
     CDS             652..1071
                     /locus_tag="IW781_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1183382.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1163..1513)
                     /locus_tag="IW781_002401"
     CDS             complement(1163..1513)
                     /locus_tag="IW781_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183383.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1479..2810)
                     /locus_tag="IW781_002402"
     CDS             complement(1479..2810)
                     /locus_tag="IW781_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1183384.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3066..3632
                     /locus_tag="IW781_002403"
     CDS             3066..3632
                     /locus_tag="IW781_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1183385.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3777..3864
                     /locus_tag="IW781_002404"
     tRNA            3777..3864
                     /locus_tag="IW781_002404"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3809..3811,aa:Ser,seq:cga)
     gene            complement(3916..5121)
                     /locus_tag="IW781_002405"
     CDS             complement(3916..5121)
                     /locus_tag="IW781_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1183386.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLFFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5259..6146)
                     /locus_tag="IW781_002406"
     CDS             complement(5259..6146)
                     /locus_tag="IW781_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1183387.1"
                     /translation="MKKMIGSLLVLASLLLFTACGKKVTTDDLKANDWLIEATKDDEP
                     NMIASFSDHIVSLSVDTSKMKSTASDEWDAMGEEFAKSLVDQMNYKFEYTLEGNVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKSNKSDDKEKLVLKPYKKEKTKDSRTTTSSTS
                     SNEQSSSTTTESTIASSSTVEQAAIAQNTPPETSSTYSAESVENYAEATPSSSVEQVS
                     STPSSTEQSVPQDYVPANQAQPTAPSSSETETLTLQPGEGPKQIAERAGITVDQLFAL
                     NGMDPNNYMLYPGQELRVK"
     gene            complement(6233..6433)
                     /locus_tag="IW781_002407"
     CDS             complement(6233..6433)
                     /locus_tag="IW781_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183388.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRAVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6447..6860)
                     /locus_tag="IW781_002408"
     CDS             complement(6447..6860)
                     /locus_tag="IW781_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1183389.1"
                     /translation="MDNIEDLLKENGIDVEITNIESEGFYLPKLRTIFINQNLDELEQ
                     KKVLLHESRHALSHNELISLYSKTVFHSKMEDEANRFMIEVLLQDYMNLFALSVDQIN
                     YMKFMDYYGIGYDCEEYIKKLLVNYVTSSMYLNVI"
     gene            complement(6872..7210)
                     /locus_tag="IW781_002409"
     CDS             complement(6872..7210)
                     /locus_tag="IW781_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183390.1"
                     /translation="MTVFERIKFLAKKHSKTMKQVTIDLGYSENYFYSLKSGKQPSAE
                     KLTEIANYFNVSVDYLLGRTDNSEDSDSQLPPTDLDDALDGAMTWNGKPLTDQDKEAV
                     RIFLQGRKSE"
     gene            7504..7695
                     /locus_tag="IW781_002410"
     CDS             7504..7695
                     /locus_tag="IW781_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183391.1"
                     /translation="MSNIDNGREAIKEFMKANNISEYDLATAYGRSRTWIQRVLSGKD
                     KGPAVNAFILEVIRDHKIR"
     gene            7706..7972
                     /locus_tag="IW781_002411"
     CDS             7706..7972
                     /locus_tag="IW781_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183392.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            8159..8344
                     /locus_tag="IW781_002412"
     CDS             8159..8344
                     /locus_tag="IW781_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183393.1"
                     /translation="MEIIYFYKDEENNIPIVVEHTNNLSSSLLNTEEFNEMIKQFPEA
                     KNNLYVLVGRTEFKLNA"
     gene            complement(8333..8722)
                     /locus_tag="IW781_002413"
     CDS             complement(8333..8722)
                     /locus_tag="IW781_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HAQ1183394.1"
                     /translation="MKLNQDCVRDILLLIEDEISFGSFLRLDFFLASEKLSKYDSETI
                     KYTLAKLDETEFLKSNTRWVNNDLASFSTGMITWKGHKFLDTIRDEKVWSNTKSIISK
                     FSSVSISMVESIASNVITQLIEKQLRV"
     gene            8775..9500
                     /locus_tag="IW781_002414"
     CDS             8775..9500
                     /locus_tag="IW781_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1183395.1"
                     /translation="MQELVILKNKEAVTTSLQVAEAFEKKHKHVLEAIESVKRSVENS
                     ANVEGVLNFEQMFVEGNEPDTYGRSRRVFFMNRDGFSLLAMGFTGSKAISFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            9679..10020
                     /locus_tag="IW781_002415"
     CDS             9679..10020
                     /locus_tag="IW781_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183396.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELACDQ
                     DDLGNFVSIWDHVYLIHLSKRSRKPEYIYVIEDGLTDALEEYERDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            10013..10747
                     /locus_tag="IW781_002416"
     CDS             10013..10747
                     /locus_tag="IW781_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1183397.1"
                     /translation="MSEGTKRNDNKLFNSLYKITVNDVVEKRNKLTYLSWAWAWAEVS
                     KICEEVDYEIYRDPETHRPYLFDEKTGYMVFTSITVNGVKRDMWLPVMDGANKAMKDK
                     PYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLGLYIF
                     AGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYDKSIDEAIVALTDAASITADDSKW
                     TKRDLGILKRGVNWLEDQYREETKEK"
     gene            10753..11445
                     /locus_tag="IW781_002417"
     CDS             10753..11445
                     /locus_tag="IW781_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183398.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            11453..11968
                     /locus_tag="IW781_002418"
     CDS             11453..11968
                     /locus_tag="IW781_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1183399.1"
                     /translation="MDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIMKPSDN
                     GKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVENLEWLS
                     SDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRHTVLNI
                     ASGKTFSFLED"
     gene            12077..12928
                     /locus_tag="IW781_002419"
     CDS             12077..12928
                     /locus_tag="IW781_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1183400.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPPKKSKAKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYQKKKALERAN
                     RQSKAPF"
     gene            12940..13209
                     /locus_tag="IW781_002420"
     CDS             12940..13209
                     /locus_tag="IW781_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183401.1"
                     /translation="MKIDYLELINEIASYKTGEEIEILRDVYEQLDEAGIERIKNDRS
                     SWSKLRYYFALYIDATQLRNLAYTKLLFADCVKGLQKHLSELEQV"
     gene            13371..13673
                     /locus_tag="IW781_002421"
     CDS             13371..13673
                     /locus_tag="IW781_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183402.1"
                     /translation="MNKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVTGTAEQLASLTGLHEKIVRKRARCGYTDVKKRTFRYVEGSS"
     gene            13670..13831
                     /locus_tag="IW781_002422"
     CDS             13670..13831
                     /locus_tag="IW781_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1183403.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
BASE COUNT         4822 a         2262 c         2707 g         4094 t
ORIGIN      
        1 acaagctcgt ttggtgtaag atgggtataa gtcatttatg ttcactctcc ttgtatgctt
       61 tagcgggtat tacaatttga gtgtaacata aatggctttt ttatttgtct cgcttaatta
      121 tacaaacggc gtaaaataaa aaaagcgtta gcaactatgc ttaaaaagca tatcgactga
      181 ataaactaag cattggacag gctgaaagac tacatttaaa atgaaatgcg aagaaacaat
      241 agaaaataaa gttgccttag aaccatagtt gaatcaccta acagtcgtga agggagaata
      301 agagaaatgg cagaaaaaca aaccgcagga cgagatcaat taggagattt tgcaccaaaa
      361 tttgcagaat tgaatgatga tgttttattt ggacaagtat ggtccagaga agaattatct
      421 gctcatcagc gtagtatcat caccatatct gcattaattt caggaggaaa ttttgaacaa
      481 ttgccagctc atctgaagat tggcaagcaa aatggcatta ctagtgaaga aatagcagaa
      541 atcattaccc atttagcttt ttatgtagga tggccaaaag cttggtcagc ttttagttta
      601 gcaaaagaaa tctatttaga agaaaatagc gaagataaga aagaggcgta aatgacgaca
      661 aaacacgaag aaatacacaa tggcgtgatt tttccagcag gtgaaaaaaa cgaagcttac
      721 gctcaagtat ttaccgggca aagctatctg aatatgttag tgaatgatcc cgaagtttct
      781 gttggtgtag gtaatgtaat ttttgaacca ggttgtcgca atcactggca tgtccataaa
      841 gacggctatc aaatcttact tgtgaccgga ggagaaggct ggtatcagga agaaggaaag
      901 ccagcaagac gattaatggc tggagatgtc gtcgtaacgc acaaagggat taaacattgg
      961 catggtgcga caaaagacag ttggtttgaa catttggcga tcactgctgg taattcagag
     1021 tttttagaac ctgtaacaga tgaagtgtat gaaggattgg aaaatcaata atgtcaagct
     1081 tgaacagctc ctgaaagtag attttaatcg aactttcagg agctgctttt ttgattcagt
     1141 cttttataaa tagaaaatag ggttaataat aggaatgaga agcacgtcga aagatcttaa
     1201 tattgataat cggctgggca tacatgattt ccacgtcatc aatcaaacta cctaacagat
     1261 gataatcttc ccgatcatgt cggggattac ctaatagttc ttgatagtag atttccatac
     1321 tagggtcccg aggattgttc agcaaatcag caagtacatc taaatcttct ggctgagttt
     1381 ctgccgtacc ctgtatctct atgaaaagcc cttcctcagc aaattctact ccaatacgta
     1441 tttcagttat ttcatgggaa tataaataag taagaagctc atcgatgatt ctttttgata
     1501 attctttttc caagtgtctt tttttccttt ctgacagcgg taaagttatc tagtatcggt
     1561 acaaggatac ctgcaacgaa accagttgaa aaaccactgt tatatagatt tagtcctccg
     1621 tgcaaagtgc tgacgttatg gacaagagca atatgtagta caccagctat tacaccgtaa
     1681 aaactgccgt aaaaaccgct tatgggcgcg agacctgtcc caaacacagc tgcaacaagc
     1741 atgccagtac tggtgatctc atgaaaagtt ccaaacaaag aagcaataaa tattccgact
     1801 agtatcggaa aactgttttt taaatgacaa ccaaaagcac tgaaacccac cgcagataaa
     1861 atagcaccga tgacaggtcc atttaactga ccgccaacca gcaaaacata agaagacagc
     1921 atcagaccaa ctaagcctat gttgatcaaa gtagcaccaa taccagaatt agcaatgaaa
     1981 tcagttgtca attttccaga tgaatcgaaa atttctcgaa tccccaaaag ccgaaaggaa
     2041 ttataataga aaccaattcc gactgtcagc gaaaaaaagc caaaaataat ccaaatgaga
     2101 ggaaagtgat attcattgaa aaccaatgta ttttcgacga ttgaatagct gaataacctc
     2161 aaaacagcag taaaagtcat cgcaatcaac ccagaggtga atccaatatt ataaagtgtg
     2221 aatccacgat ggaaaactaa agtttgagca gctaagggcg gaagaagtag accaattgct
     2281 atgccagcta aattccccaa taaaatacct atcagtaaag gaaaacgcat accaaaagtg
     2341 atataactaa cgaccggtga taatgcgcta ccaaacagtc cgatcatgat gtactgagaa
     2401 aaaggtttat tgacaaatac agcgtataga tacacaccta aaatgattga gatagaatta
     2461 taaaggtttt tgccaaaaaa agagaaaccg gtaactgtta gaatcgaagc aaataaggga
     2521 ccgtttaaaa tcaccttgtg ccgataagct aaaagcacag aaagtaaggt caaaatccct
     2581 acatttatga atgcgcttcc gaaaccgcct aaagcaaaat aatctgtcaa aagattgctt
     2641 ggcgaggtaa gaatagttag caatccactc caattatcag taggagtatt agaaagcaag
     2701 ccaataaaaa gaaaaaaaac acctgtaaaa ccaagataaa aatactttct tttctgtgaa
     2761 acgattcgtt tttgaggatg aatataaata acgtcagttt cgttttccat tacacacctt
     2821 ctttttcatt ttctttaact ataacttgaa atgaaagatt tggacaacta tcatttaggt
     2881 caaaactaaa aatgtaaggg aacccattga atttaaccaa atttttttaa ttttgcaaaa
     2941 aaaagttaaa gaagactgaa aaaaaattta aaatataacg ttttgagtaa aatgtttgca
     3001 aaaaatgcta aaaatagggt acaataaaac aacatatact gcagtaaaga agtgggggag
     3061 ttttaatgag aagaaaagtc tataccaagg atcaaattct aaaagctgct tatgatgttg
     3121 ttgcaaagga agggtttaaa ggatttactg cgagaaatat tgctaaaaag atggggattt
     3181 cgacacagcc gatttatctg gaattcaaaa acatggaaga tttaaaaaat acgttgctgg
     3241 attctatctt tgaacaacta aaagaagatg ttttccctat tgaacgtact ggtgataaaa
     3301 tcattgatct cggtttgaac tatatctact ttgcaaaaaa gcatcatagt ctttacatgg
     3361 cattgtacat tgatgaacat ggggacggaa agaaaatgca tgatttttca tacaattatt
     3421 tccgtgaaat agttaaagaa gatgaaagat acaatcaatt atcggatgaa tatgtcgatg
     3481 ctctgcatac tggtacatgg gtagcagtga ctggcttagc gtcattgatg tcatcaaaca
     3541 tcatgcatcc aacagaagaa caaatcattg cactgatcaa acggacaatt gactcgattt
     3601 tagcattaga taatcctgaa ataatcagat agtgttctat cagtatgaat ttgatcggta
     3661 agtccgttgg ctgcaaactg tcaatggact tttttgctaa aaaaagcgat ttctgttatt
     3721 tatctattag aaataggctt gacatttcgt gaaaaaagtt taaaaatagt acttacggaa
     3781 ggttggcaga gtggtaatgc accggactcg aaatccggcg aaccggctaa taccggcgcg
     3841 caggttcaaa tcctgtacct tccttatata tcaacgatta gacacgattt cgatcgtgtc
     3901 tttttttgtt gtaatttagc ctaaaatgtt ttttgactcc ccttttgact ccccctgagt
     3961 actattcttc ataaatgcta caaaattatt gatgactttt ttgttttgag tttcagttac
     4021 atgagtgtaa atatttgaag tagtttgaat gtctgcatgc cctaaacgtt cctgaacatc
     4081 ttttaaagaa gctcctgatt caaaaaggag agaggcatga gtatgtcgaa aaccatgagt
     4141 agtgattctt ttagttaatt ctggatgatg cttatatatg acattaagcc acgaacgtga
     4201 aactgtaggg ttatagagaa gaccatcatg gtgaaaaaat aagttttcgc tttggattgt
     4261 aacgatcgtt ttgttttttg tataatactt tctcaataaa ctgatcaaat cattgtccag
     4321 ataaattttt ctttttccag attttgattt aggagtattt acgatcaatc tcccttgata
     4381 acctcttgtt acagttttat taatattgag tgttttgctc ttaaaatcaa tatccgacca
     4441 ggtcagtgca aaagcttctc cttttcttat acctgtaaaa gccaataggg aaaagaataa
     4501 atatctttca tcatcatcgt ttcttattgc ttctaaaaat tcgattaatt cttctttagt
     4561 ataaaattcg atatttttat cttcgatatc tagagcttct ccacgtggaa tagaaactaa
     4621 tttcatagga ttgctagtta taatttgtaa agaagcagcg taatcaaaaa cattagatgt
     4681 gtaatttttt attttcttaa aaattttagg atggctatcg gaccaagtgt ttactgcttc
     4741 ttgacagaaa aaaacgtcta ttttgtcaat gaacttatct ccaaacactt ttaagatgtg
     4801 tgtgtcaaaa atttgtttgg tggaggacca agtgctttct ttaactgtct ttttataatt
     4861 ttcaaaccat aagccgtaaa catattcgaa tttcttgctc ttttgagctg tagaagcttg
     4921 cagtcctttt tcctgtatat ctgcctccaa tctttttaaa gctctctcag cggctaatgg
     4981 cgtactaaaa cctcgtctcg ttgtttttct tttttttcct gttagaggat caactcctaa
     5041 gtacagacta aactgatatt tttcttcgcc tttttttgtt aagtgctttt taattcgttt
     5101 atcaatttct ttttttgcca tctttatctt tcctttcgta cgtttgttcg gttgtacagc
     5161 ggatttcgag atggtaaaat agggtacaac aaatagacct actttaccgt agctctttgc
     5221 acatttgcgt tcttggtcgg gcggcaaatg tgcttttttt atttcactct tagttcttgt
     5281 cctggataaa gcatgtagtt gtttggatcc ataccgttta atgcaaacag ttgatctacc
     5341 gtaattccag ctcgttcagc tatttgtttg ggcccttcac caggttgcaa agtcaaggtt
     5401 tcggtttcag atgaagaagg tgccgttggt tgagcttgat tagctggtac ataatcttgt
     5461 ggtactgatt gctccgttga actgggagtg gatgaaactt gttcaacact tgaactagga
     5521 gtagcttctg catagttttc tacactttca gcagagtaag tactactagt ttcaggaggc
     5581 gtattctgcg caatagcagc ttgttctaca gtactagaag atgcaatagt actttctgtt
     5641 gtagtcgaag atgattgttc gttgctactt gttgagctag tagttgttct agagtcttta
     5701 gttttttcct ttttgtaggg ttttaaaact aatttctctt tatcatcaga tttattagat
     5761 ttttctggag tcaaaataat gtttttgtct tctttgctaa cggtatactt ggcatcgtta
     5821 tccttgtctt tgtcgtcttt ccaagtcata acattacctt caagagtgta ttcaaattta
     5881 taattcattt gatcaactag gcttttagca aattcttctc ccatggcgtc ccactcatcg
     5941 ctagctgtag atttcatttt gcttgtgtca acactaagag aaacgatatg atcagaaaat
     6001 gatgctatca tatttggttc gtcatctttg gttgcttcaa tcaaccaatc attagctttt
     6061 aaatcatctg tggttacttt tttcccacaa gcagtgaaga gtagtaggga agcaagaact
     6121 aacagacttc ctatcatttt tttcattttt tattcctcat ttctatgata tgatttttat
     6181 tggggaatct tagaaataag gtttcgagtc cgtgttgctg cacgggcttt ttttatttaa
     6241 ataaatccca aaaactgaat gtagttttct tgtatacttt attgtaagca gccttttttg
     6301 gatctttgat ccaaccagat ccctttttac cgtaaccagg aatcactgct tttttcacag
     6361 ctctttttgc ctttccagta gttctagcgc taatggattt ttttatactc ggttttctca
     6421 ttcctatttt cataagaagc ctccatttaa attacattaa ggtacataga agaagtgaca
     6481 tagttcacaa gtaatttttt tatatattct tcacaatcat aaccaattcc ataataatcc
     6541 atgaatttca tatagttgat ttgatctaca gataaagcaa ataaattcat ataatcttgc
     6601 aacagtactt caatcatgaa tctatttgct tcatcttcca tttttgagtg gaatacagtt
     6661 ttgctgtaaa gtgatatcaa ttcattgtga cttagtgcat gacgtgattc atgtagcaat
     6721 actttttttt gctctaattc atctaaattt tgattaatga aaattgtcct taatttagga
     6781 agataaaatc cttctgattc aatattggta atttctacat caatcccatt ttccttcaac
     6841 aaatcttcaa tgttatccat acactacaac ctcactcact ttttctacct tgtaaaaata
     6901 tccttaccgc ttctttatct tgatcggtaa gtggttttcc gttccaagtc attgctccat
     6961 ctaaagcatc atccaaatcg gttggtggta attgagaatc actatcctcc gagttatcgg
     7021 ttctacctag tagataatca acagatacat tgaaataatt agcaatttca gtaagctttt
     7081 cagcggatgg ttgttttcca ctttttaaac tatagaaata gttttcactg tatcctaaat
     7141 caattgttac ttgtttcatt gtttttgaat gtttttttgc aagaaatttt atccgctcaa
     7201 atactgtcat accagcattc tccttttttc tttacaaaaa accaataaaa aagtgtagtt
     7261 ttgtgttgac ctaaacaaca ctatagtgta tattggtttt gtaagttaat tggatagaaa
     7321 aaaagcaaag taaaaacaca ccttatagca ttaagtttgg cgaccgagtg cgataaaaaa
     7381 aggtttgtta taggcttatt taactatggc tatatactac actatagtat agttcgtagt
     7441 caactaaaaa tatacttttc tatccaattt tctttctaaa taaaaagaaa ggaagtgtgt
     7501 gaagtgagta atatcgataa tgggcgggaa gccatcaaag aatttatgaa agcaaataat
     7561 atttcagaat acgatttggc cactgcatat ggtagatcga gaacttggat tcagcgtgtt
     7621 ttaagtggaa aagataaagg tccagctgtt aacgccttta ttctggaagt tattcgcgat
     7681 cataaaattc gataggaggc aagtcatgaa tattctaagc gaagagtttc tgactcgatt
     7741 gagaatcgca attgttgaag ttgtaaagga cgcacttagt caactttcaa aaaagaattt
     7801 gtcagaaaca cgatatttaa aaaagatcga agctagaaaa tatgtgggag gtgtaaacga
     7861 tcaaggcttt gagaagttaa tagctcacgg tttaaaagaa attcgtatag atggcttttt
     7921 gagatatgac aaaaaggaca tcgatgaact gatggctaaa tacaaaattt aaaaggaggt
     7981 caacatggga aaatttaaca gagcattagt attcagcgca ccgctaatca tctacgcttt
     8041 aggactttgg ggaaatagac aagcgttgat aggaacgatc gtttacatgg tttggatttt
     8101 tatagggctg gatgaagctg agtatagagc gaaaaagcca gtcgggaggg actgactaat
     8161 ggagattatt tatttctaca aagatgaaga aaacaatatc ccaattgttg tcgagcatac
     8221 taataaccta tcatcaagtt tattaaacac tgaagaattc aatgaaatga taaagcaatt
     8281 tcctgaagca aaaaacaatt tatacgtact ggttggaaga actgaattta aattaaacgc
     8341 gtaactgttt ttctataagt tgagttatta cattggatgc gattgattct accatgctta
     8401 tagatacgct tgaaaattta gagataatcg attttgtatt tgaccaaact ttttcatcac
     8461 ggattgtatc taaaaatttg tgacctttcc acgtaatcat tccagtagaa aaagatgcta
     8521 aatcattatt tacccatcta gtatttgatt tcagaaattc tgtttcatca agtttagcaa
     8581 gtgtgtattt tatagtctca ctatcatatt tcgatagttt ttcagaggct aagaagaagt
     8641 ctaatcgtag aaaacttcca aaagaaatct catcctcgat tagaaggagt atgtcacgta
     8701 cgcaatcttg atttaatttc attataaaca ccaccagttt ttacctaaat tataccaaaa
     8761 ggagagaaat aaaaatgcaa gaattagtaa ttttgaaaaa taaagaagct gtgactacta
     8821 gcttgcaagt agctgaagct ttcgaaaaga aacataagca tgtgctagaa gcaattgaat
     8881 cagtaaaaag atcggtcgaa aattcggcca atgttgaagg tgtgctcaat tttgagcaga
     8941 tgtttgtaga aggaaacgag ccggacacgt atgggagaag tcggagagtt tttttcatga
     9001 atagagatgg attttccttg ttagctatgg gattcactgg aagtaaagca ataagtttca
     9061 aactcaaatt tattgaagca ttcaacgaga tggaagatgt cattcgtaaa aatactgttc
     9121 ctcaaacgat tgaagacatg atgatctacc aattagaaga aatgaaagat gtaaaaaaag
     9181 atgtctctat gcttaaagat actatgcgaa ttagcggaca acaagagttt gaaattaagc
     9241 aaaaaggaaa tatgaaagtt atggaagttt taggaggtaa agaaagccga gcttatgaag
     9301 aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc
     9361 caagatatgg cgagttacct cgtaagagat tcgatgatgc tgtttcattt attgaaatgt
     9421 ggttaccaga aactgcgatc cgcatggaaa tcgatcaact gaacagacaa caaagacttt
     9481 tcggtgatga aaatgaatag agctgaagcg ctaagaatag ggacggtaat tgctaatcgc
     9541 tggtggagac acaataaacc aagcatccta agccaacaac atattgataa gcaaaaagct
     9601 tggcaacaaa taaaaaagtg actcagccgg caagcataga gtcacaaaac aaaatatatc
     9661 taaggagaat tttagcatat gaataaagaa ctttccactt tagatcaata tttgactgat
     9721 tctgaatggg gcaagtcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt
     9781 ttgacgaacg aagaactagc atgtgatcaa gatgatttgg ggaattttgt gagtatttgg
     9841 gatcatgtct atcttattca tctatcgaag cggtccagaa aacctgaata catctatgtc
     9901 atcgaagatg gcttgactga tgcattagaa gagtacgaaa gggataactt gattgatatc
     9961 tcttattacg gaccaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga
    10021 aggaacgaaa cgcaacgata acaaattatt caatagtctg tacaagataa ccgtcaatga
    10081 tgttgtcgaa aaaaggaaca aactaaccta tttgtcttgg gcatgggcgt gggcagaagt
    10141 cagcaaaata tgcgaagaag tagactacga aatctatcgt gatccagaaa cgcatcgtcc
    10201 atacctcttt gatgaaaaaa caggctatat ggtttttacc agtatcacag tcaacggagt
    10261 aaagcgtgac atgtggttac cagtcatgga tggtgcaaac aaggcaatga aagataagcc
    10321 atatacctac gaagtcaatg attatcagtg gaataacgaa acgaagaaaa aagagattgt
    10381 tggaaaaatc gaaaagcgag ttgaagcagc aacgatgttt gatatcaata aaacaatcat
    10441 gcgctgtctt gtaaaaaatc tagcgatgtt tgggctaggg ctatatatat ttgctggcga
    10501 agatatgcca gaagacgtct caatgcttga accagctact caaagaagca aaaagctatt
    10561 cttagatgct ttgcaattgg ttgctaacaa gtacgataaa tcaattgatg aagcaattgt
    10621 tgcattgact gatgcggctt ctataaccgc tgatgacagt aaatggacca agagagactt
    10681 gggcattcta aaacgaggcg ttaattggct tgaagatcag tacagagaag aaacaaaaga
    10741 gaagtgatat gagtgtttaa accattaatc gattcatatt cagcagttct gaaaaagttc
    10801 aaaggaaaag acataggtgc aacgatcaat gaagaagtga acattgatcg actaaagacg
    10861 atgtatgacg gctacgatgg cgatcgagtc attgaaattc gttttattga tcctagacgt
    10921 ttcactgtac agcaacgaaa cttcatctat gcgctgatag gcgatatttt catcgataca
    10981 ggcatgccaa cggacttctg gaaggaattc ttctacttcc gtttcgaagg tgtcacaggg
    11041 cgcaaaataa gcctcaaaga tgaatcgagc acaaccgtaa gtgatgccaa tatcttagcg
    11101 aatatcatcc tagatttcat ctttgagcat gatattccgt ttaaaaatgg atatgaaatt
    11161 ttgccccaaa acgaacagta tttcttttac aaatgcctca cgaacagggt gtgctgtagt
    11221 tgcggaaaga aaaatgctga tatacatcac gtagactctg tcggcatggg aaataaccga
    11281 aaaaaaataa acaattctgg taggcgcttt atggctctgt gcagagaatg ccatacgaaa
    11341 attcatgtcg aaggttttac tacatttaca actaaaagaa agcttacggc agttgttctc
    11401 aaagacagtg atttaaaaag attggggttg aatatcattg aatgaattat ggatggatat
    11461 cgaaggatac gagggagtat atcaagttag tagcttagga agagttaaat ctaatcgccc
    11521 taactatgta attaataagg gatacgttaa caaagaagga tttattatga aaccatctga
    11581 caatgggaaa gggtatcaaa ttatcttctt aagcggcaaa ggttttagag atagaaggta
    11641 tgtccatcgg ctagtagctt ttcacttttt aaaagaagcg tatttcgaag gtgctgaagt
    11701 caatcataag aacggagata aatctaacaa tactgttgaa aacctagaat ggctcagttc
    11761 tgatgataat aaaaagcatg ctagaaaaaa tggattaact aacttgaaag gaccatcaaa
    11821 actaactgat ttgcaagctt tagctatcaa gcgtttatat acaaacaaat tgatgagctc
    11881 cggagaaata atgaaacttt ttaatgtcag caggcatacc gtgctgaata tagcttcagg
    11941 gaagacattt agtttcttgg aagattaaga gaaacacaaa ataggtgtga ctaatttcaa
    12001 aaataagtat caaatcaaag ggattaagtt aaaccaggaa acaattaaga aacttagaat
    12061 aggaggataa aaaatattgg ctgacaacaa acgatactac tatttaaaac taaaagagaa
    12121 cttcttcgat agcgacgaga tggttctttt agaaagtatg ccagatggat acatttattc
    12181 taatattctt ctcaaacttt atttaagaag tctgaaacat gaaggcaaac taatgtttaa
    12241 tgacaggatt ccattcaatt ctacaatgct tgcgactatt acaagacact ctgtgggagt
    12301 tgtagaaaaa gcagtacaaa tatttcgtga tttacaactt attgaggtat tagataacgg
    12361 agcaatttat atgtctgata tacaaagttt tattgggaaa tcttcaactg aagctgatag
    12421 gaagagaaaa tacagaaaag aaattgagga agcaaaacag aatttaataa caagtggaca
    12481 aatgtcggac aaatgtccgg acaaaactac accagagtta gagatagagt tagagaaaga
    12541 tatagagtta gagaaagata tagagaatgt aacgcctccg aaaaaatcga aggctaagcc
    12601 catccgtcat aaatacggag agtataaaaa tgttcttttg tcagatgagc agatggagaa
    12661 actcaaaaca gaattcccta atgactacca agagcgaata gaacggctat ctgagtattg
    12721 tgaatcatca ggtaagactt ataaaaacta tttggcaact attcgaagtt gggcaaggaa
    12781 agaaaaaagt gagcctaaga atgcaagtgg tgcatacaaa cgcacaggac gacgagagaa
    12841 gcttccagaa tgggcaatcg accaagaagc ctatcaaaag aaaaaagcgc tagaacgagc
    12901 taatagacaa tcaaaagcac cattctaaga ggtggaaaat tgaaaatcga ttatctagaa
    12961 ctgatcaatg aaatagcaag ttacaaaact ggtgaggaaa tagagattct gagagacgtt
    13021 tatgaacaac ttgatgaagc tggaatcgaa cgaattaaga atgatcgttc aagttggagt
    13081 aaactcagat actatttcgc actttatatc gatgcaacac aattaagaaa tttagcttat
    13141 acaaaattac tatttgctga ttgcgttaaa ggattgcaaa aacatcttag tgaacttgag
    13201 caggtgtaat cagatggatc taaaaacatt tacagcacag atcgaactaa tgcatcaaga
    13261 agctttaaga caaagcgcct cgtacgaaga caagtggctc aacacgttcc atggtggacg
    13321 tgagagcgca cttgatcaag tgctcaaatt attgaaagga gaatgtcggg atgaataaga
    13381 aagcggcaat gcagcgaatt atcgaattga cttattcaga agattggcaa aatgacaaag
    13441 aagctgcttc agaagtgatg agacttggaa gagcgatgtg ggcagacaag agcaacaagc
    13501 caagaccgcg aaaaatcgca atttggcacg gtgacaaact tctagtgaca gggacagctg
    13561 aacagttagc aagtctcaca ggcttgcacg agaaaatcgt gagaaaaaga gctaggtgtg
    13621 gatacacaga cgttaagaag agaacgttta gatacgtgga gggatcgtca tgacaacaga
    13681 agaagtgatt caaatgcgta ttcgaaacat tcagcgtgaa attgacgatc tagaacgaac
    13741 aaaggcagtg atggtcaatg aaacggctag aaaggcaatc gatttgcaca tagagaactt
    13801 aagaagggaa attcggagat tggaggaatg agcgtggata agaaagcagc aatgaaacga
    13861 atcatcgaac tgacacattc tgaga
//
LOCUS       DACEOM010000080        13807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEOM010000080 DACEOM010000000
VERSION     DACEOM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.083"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW781_002423"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW781_002423"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1183404.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IW781_002424"
     CDS             complement(2260..2511)
                     /locus_tag="IW781_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1183405.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IW781_002425"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IW781_002425"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1183406.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IW781_002426"
     CDS             3461..3679
                     /locus_tag="IW781_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183407.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IW781_002427"
     CDS             complement(3968..4480)
                     /locus_tag="IW781_002427"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1183408.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IW781_002428"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IW781_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1183409.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IW781_002429"
     CDS             complement(6933..7367)
                     /locus_tag="IW781_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1183410.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IW781_002430"
     CDS             complement(7377..8165)
                     /locus_tag="IW781_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1183411.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IW781_002431"
     CDS             complement(8162..9103)
                     /locus_tag="IW781_002431"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1183412.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IW781_002432"
     CDS             complement(9280..9867)
                     /locus_tag="IW781_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1183413.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IW781_002433"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IW781_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1183414.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IW781_002434"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IW781_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1183415.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IW781_002435"
     CDS             complement(12784..13719)
                     /locus_tag="IW781_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1183416.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DACEOM010000081        12971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEOM010000081 DACEOM010000000
VERSION     DACEOM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.084"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            65..2101
                     /locus_tag="IW781_002436"
     CDS             65..2101
                     /locus_tag="IW781_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1183417.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEQPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSDVSQVDLKTALIYSDNIYTAQETLKMGEKKFRTGLDKFIFGE
                     DLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFANNGTLVYP
                     KLIADKETKDKKNVIGETALQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEIK
                     EKQDVKGKENSFLFAFNPDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2210..3361)
                     /locus_tag="IW781_002437"
     CDS             complement(2210..3361)
                     /locus_tag="IW781_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1183418.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(3515..4045)
                     /locus_tag="IW781_002438"
     CDS             complement(3515..4045)
                     /locus_tag="IW781_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183419.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4144..4494)
                     /locus_tag="IW781_002439"
     CDS             complement(4144..4494)
                     /locus_tag="IW781_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1183420.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(4651..6180)
                     /gene="ahpF"
                     /locus_tag="IW781_002440"
     CDS             complement(4651..6180)
                     /gene="ahpF"
                     /locus_tag="IW781_002440"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1183421.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6190..6753)
                     /gene="ahpC"
                     /locus_tag="IW781_002441"
     CDS             complement(6190..6753)
                     /gene="ahpC"
                     /locus_tag="IW781_002441"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1183422.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(6897..7559)
                     /locus_tag="IW781_002442"
     CDS             complement(6897..7559)
                     /locus_tag="IW781_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1183423.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            7743..9389
                     /locus_tag="IW781_002443"
     CDS             7743..9389
                     /locus_tag="IW781_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1183424.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILANKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            9380..10462
                     /gene="uxuA"
                     /locus_tag="IW781_002444"
     CDS             9380..10462
                     /gene="uxuA"
                     /locus_tag="IW781_002444"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1183425.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(10570..12123)
                     /locus_tag="IW781_002445"
     CDS             complement(10570..12123)
                     /locus_tag="IW781_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1183426.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12271..>12971
                     /locus_tag="IW781_002446"
     CDS             12271..>12971
                     /locus_tag="IW781_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1183427.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDV"
BASE COUNT         4390 a         2406 c         2380 g         3795 t
ORIGIN      
        1 atgttagaat aaacaggtat aaatagtgaa ataaaggaat gacaagcaag agaaggagga
       61 aaaaatgaaa agaagtgaca agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc
      121 agtgatctta atagcagctg cgggtggcgg ttatttttac taccagcact atcaagaaac
      181 ccaagcagta gaagctggag aaaagacagt tgagcaattt gtccaagctt tgaacaaggg
      241 agattataac aaagctgcag aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc
      301 tgaaaaagag atcttagata aataccagaa tatatacggt gctgctgatg tcaaaggact
      361 tcagatatcg aatctaaaag tagataaaaa agacgattct acttacagtt tttcatataa
      421 agcaaagatg aatacctcat taggtgaatt gaaagatctt tcttataaag gaacattaga
      481 cagaaatgat ggtcaaacca cgatcaactg gcaacccaac ttggtttttc cagaaatgga
      541 aggaaatgac aaagtaagcc tgaccacaca agaagcagca agagggaata ttatagatcg
      601 aaatggtgaa ccattagcaa caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa
      661 acttggagat ggaggcgaaa aaacagccaa tatcaaagcg attgcttcct ccttcgactt
      721 aacagaagat gctatcaatc aggcgatttc acaaagctgg gtacaacccg attactttgt
      781 cccattgaaa atcattgatg gagcaacgcc agaacttcca gctggagcta ccattcaaga
      841 agtagacggc agatattatc ctttgggtga agcagctgct cagctgattg gttacgtggg
      901 agatatcaca gcagaagata ttgataagaa tccagaatta agcagtaatg gaaaaatcgg
      961 tcgctcaggt ttggagatgg cttttgataa ggatcttcgt gggacaacag gtgggaaatt
     1021 aagcattaca gatgcagacg gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa
     1081 tggcaaagat atcaaattga caatcgatgc gaaggcacaa aaaacagctt ttgacagtct
     1141 aggaggaaaa gctggatcaa ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct
     1201 tgctagctct ccaagctatg atccaaacaa aatgacaaac gggatctcac aagaagatta
     1261 caaagcttat gaagaaaatc ctgaacagcc attcatcagc cgatttgcga caggttatgc
     1321 tcctggatct acgtttaaaa tgattacagc agcaatcggt ctcgacaacg gcactatcga
     1381 tccaaatgaa gtgttgacga tcaacgggct taaatggcaa aaagacagtt cttggggatc
     1441 ttatcaagta acgcgtgtca gtgatgtatc acaagtagac ttaaaaactg ctttgatcta
     1501 ttccgataat atatatacgg cacaagaaac gttgaaaatg ggtgagaaaa aatttcgtac
     1561 aggcttagat aaattcattt ttggtgaaga ccttgatttg ccaattagta tgaatccagc
     1621 acaaatttct aatgaagata gctttaactc agatatcttg ctagctgata ctggatatgg
     1681 acagggcgaa cttctaatta atcctatcca gcaagcagca atgtattctg tttttgccaa
     1741 caatggcaca cttgtctatc ctaaattgat tgcagataaa gagacaaaag ataagaagaa
     1801 tgtcatcggc gaaacagcat tacaaacgat cgtgccagat ctgagagaag ttgtgcaaga
     1861 tgtaaatggt acagcacatt ctctttctgc tttagggatt ccgttggcag cgaaaactgg
     1921 tacagcggaa atcaaagaaa aacaggatgt aaaggggaaa gagaacagtt tcttgtttgc
     1981 tttcaatcct gataaccaag gttatatgat ggtcagcatg ttggaaaata aagaagatga
     2041 tgattcagca accaaacgag catcggaact attgcaatac ctcaaccaaa attatcaata
     2101 aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt atcgtcacag tccttttttc
     2161 aaataaacgg ttttcacgat gtctctttca caacttcttt ttgatttact cacacttcat
     2221 aaactttttt tgtaaaatat ttgagaacaa gtggagaaat aagtgtactt aataaaatca
     2281 cgataactaa aggtgaatag tactgtggtt cgatcaattg actttgctgt ccaatttgta
     2341 aaatgatcaa agccatttct ccacgcgaaa tcataccagc accgaccatc aatgaactat
     2401 gttgagaaaa accggcaatc tgagcaccaa aataacctcc aagcaatttg gtcaaaatcg
     2461 ctacaagcgt caatatagaa ataaagagta gttgttcact aaagtcagaa aaatcaactt
     2521 ctaatcctac actgacaaaa aatactggta taaatactgc atatcctaaa gcctctacat
     2581 tgtggtatac ttcttcttta acctttgtct gactgacagc tatcccagca aaaaaagcac
     2641 caatgacaga gctaagccca acaagatccg ctacgtaaga cattcctaaa caaataacta
     2701 gcgatacgat gataatagaa gaatttgcgt acactttttc tgcaaatgaa agtaggaaag
     2761 gcgcaatcca tttgactaat aagaaaataa aaacaaaata aattagctgt tcaatcaaaa
     2821 caagcggtaa agaagcattg ctagaagctt cccccgttag aaaagatagg ctgaaactta
     2881 gaactaaaac gactaaaatg tcatctacca cagaagcacc caaaattgtt gatccttctt
     2941 ttgtattcac tacgtttagt tcttttaata cttctactga aatactgaca gaagttgccg
     3001 caagaataat cccaaagaaa atcgcttcat ttcccgaaac ttggaatgct ttgcctgtca
     3061 accagccaaa aacaaccggg aagatgatcc caagcaaagc aacgaacata ccaggtcgga
     3121 aatatttttt caaaagagat aaatcacttt ccagaccagc taaaaacatt aataagatca
     3181 caccaatctc cgaaaagtca tggactagaa tatcaggttt gacccaattc aatccagctt
     3241 ggcctagcaa aacaccaaca agtaattgtc caatcactgc tgggatacct aatctccgcg
     3301 agatatgtga gccaatcgtt gttgaaatca atatcaggca taaaatacca ataaattcca
     3361 ttctcatcat tcctttgtat aaaatatagc ccgaacttag cataacatac attattgaaa
     3421 aaataggaaa atatactcac aaaaaaacca acaatccaaa agattttttt ggattgttgg
     3481 tttttatacg gtctatttta ataactaaaa atgattaacg tatagtcatt tttcttattc
     3541 tgatgaaatt gataatctcc tggatgccca tgaaaatcaa aataaagccg aatatttgga
     3601 atagaagcat caaacttttg aatggattga acaataagac gaatccgcca atcaacaaca
     3661 gaatactact ataaacactc caacttttag cttttgtatt catgctgaag aacaactgga
     3721 acaagctgtt gatgataaca gctaatccaa gaagaatcgg caggatagaa acgattgcag
     3781 gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa aagaccactg aacagaccga
     3841 tactccaaga acctgttttc cttttaaaac ggtaagcctc aaaaagattc aacaacccaa
     3901 gcaaaacaag ataggcggtg atcagataac cgataaaatg aaaaacagca gaagggttta
     3961 gcacaatcgc aatcccagaa ataatataaa cagcagctct taataaggcg tatttttgaa
     4021 tattttgttt aatcgtctca aacatataag aaaacctcct ttgacttatt ttaacgtgaa
     4081 ctagtcattt taccaaatta aatgttacaa aatgagcgaa ggagaagaat atctctggaa
     4141 aaatcaactt ctgaggatct tttccatttt tttgcctttt gctagctcat cgatcatttt
     4201 atctaaataa cgaatttctt tcatcaagtt atcctcgatg tcttctactc gtacaccaca
     4261 aacaacacct gtaatcaagg aacgtgaagg attggggaca ggggcttccg caaaaaattg
     4321 ttcgaaagtc acttgttttt gtagttgata ttctagttct gtttgggaat aacccgtcag
     4381 ccaacaaatg atttcatcta cttcctcttt tatacgatct tttctctccg cttttgctac
     4441 atacatagga taaacgcttg caaaacttgt cgtaaaaata cgattttgcg ccataatcag
     4501 tcacctttct ttttttcaaa ttatttacta cttatagtat ataagaaaaa gagaagaaaa
     4561 agcgattatt caaaaaaaca aaaaggattg tgacaaaatt cttgtcacca tccttttttc
     4621 cgctctctga ctttttacaa tcttcatagc tcaagtgcgc atcagataat cgaatgctcc
     4681 taaagctgca gtagctcctg aacccattga aacaatgatt tgtttgtatg gactatctgt
     4741 acaatcacca gctgcgaaaa cgccggggat attcgttgcg ccatttttct cggtcactat
     4801 ttcatttctt ggattcattt cgatcgttct atctagccaa tccgtattag ggagtaagcc
     4861 gactagaatg aatactcctg aaacagctag tgtatgcatc tccatcgtac ggcgatcgat
     4921 ataggagaga gattctacct gattcgtacc gctgatttct ttcgtcgctg catgtttgat
     4981 aacagtgaca ttcgcaagag agactagttt atcctgtaag atttgatctg cttttaattc
     5041 tggtaagaat tctagaacat atacatgttt tgctaaccca gccaaatcga ttgctgcttc
     5101 aacaccagaa ttaccaccac cgataacagc aatctctttt cctttaaata aagggccgtc
     5161 acagtgtggg caataagcaa ttcctttatt tttaaactct ttttcgcctg gtacattgat
     5221 agaacgccag tgcgcacctg tcgcgatgat ggcggttttt gctttaagtg atataccgtt
     5281 agctaagcca atattaataa aatgttgctc tttatttaag gaaacggcct gttgattcat
     5341 cataatgtcg atagggtaac tacgcacatg actttctact tgctgcatca attttggacc
     5401 ttctgtgtaa gaggtaccaa tcatattttc aatccctagt gtttcaacga cttgtccgcc
     5461 gaaagaatca gcgacaagtc ctgtacggat tcctttacgt gctgcataga tagctgcact
     5521 actagctgct ggtccaccgc caatcactaa tacatcataa ggttcttttt caaacaactc
     5581 ttcttcaatc aaagggccag tgattttttc aacaatttgt tcgatcgtca tgcgtccgct
     5641 actaaattct tctccattta aaaacacagt tggcactgcc atgatttttt tatcatcgat
     5701 ttctttttgg aacatcccac cttcaatcat cgtatgagta atatttggat ttaatacagc
     5761 taatatattc aatgcctgta caacgtcggg gcaattatga caagtcaagc taacataact
     5821 gtcaaaatgc aatgtttgtg ttattttttt gattttaaga ctcaaagatt cactgatttt
     5881 cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa gaggaaaatt catgaccgag
     5941 ggggatacca gcaaaaacga ttccactttc tttgccaact ggattgatag aaaaactagg
     6001 tgtgcgcatc gaatctcttt tttcccaaga gattttagga gacatttctg ctatttcctt
     6061 cgtaaattca gctagttttt gactattttc actattatcc aaactaacag aaaaaacaat
     6121 aggagattca atcagtgcta aatattgttc gagattttgt ttagtttctt tgtctaacat
     6181 atccaccacc taaatctttc caacaagatc aaaacttggt ttcaatgttt cgccactttc
     6241 tttccatttt gccggacaaa cttcacctgg gtgggtacgt acatattgag cagcgcgaat
     6301 cttatcaatc agtgcactag cgtcacgacc gattccatct gcattgattt ccatagcttg
     6361 aacgattccg tctggatcaa taataaatgt tcctcgctga gctaatcctt gctcttcatc
     6421 caaaacatca aaaatacgag aaatctgatg agacggatca ccgatcatca cgtattcgat
     6481 tttaccgata gcatctgatg tatcatgcca tgctttgtga gtaaagtggg tatcggtaga
     6541 gcaagaaaag acttcaacac ctaaagattt caatgtactg tattgttctt gtaagtcttc
     6601 taattcagta ggacaaacaa aagtaaaatc agctggatag aagcaaagaa tactccagtt
     6661 ccccataaga tctttttcac tgacttctaa aaattctcct tgatgataag catgtgcatt
     6721 aaattcttcg atttttttcc caatcaatga catagtaaaa cctccaataa taattattta
     6781 tttataatta ttgtaattta gttggagttg caaagtcaat ggatattttg ttatttaata
     6841 aagattactc aaaaacgaag gttaattata aaaatagaag aggtcgcaag aaaatgctac
     6901 tgctggaaat actcgggata acgttgagta acagtttcct gttctttcag catcatgtgt
     6961 aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt tatcaactaa agccttgagt
     7021 aaatcataat gttgttccat gatcgtttcc catggaagcc cctgaatggt cactcgtaac
     7081 cagcggaaac gttctaaatg ggtattatgg tcatctaacc agttccaaac ttcttttctt
     7141 cctgcaattt cgaaacaagc ttcatgaaat aaattatctg cacggaaaaa agcgcgttcg
     7201 tcatttattt tgactgcttt ttcttgttct tctaaaatcg tttctagcac tattcgtgtt
     7261 tgtgtgtcca atttggaaca acattcaatc atgatttgct cttccaactg ttctcggaca
     7321 aagcgagcat tttctgcaga aacaagatta atttttgaaa catacgtacc actttgaggt
     7381 atcgtataca cgagttcttg ctgacgtagt tgaatcaatg cttcgcgaat gggagtccgg
     7441 ccgatagcaa gatgttcctc cagtgccttt tcagatattt tccttccagg ttctaagtca
     7501 gcatagataa tttgtttacg aatcgcttga taagcttgag attgcaagct gcttttcatc
     7561 tttattcctc caaaatcatt tctatgaacg tagtataaca gaagaggtct gaaaaacaat
     7621 ctggtatgtc acattaacta gtaaatttgt ataaatagta aaattttggt tgacatagcg
     7681 atttttattg cataaaattc acttgaatac aaaaactaat atgttagttt aggaggggaa
     7741 aaatgattga tttaaacaac tggaggaact ttatccctga attatctcaa aaagaagtag
     7801 aaatacctca ttttgaccgt gcatcattaa gagaaaatgg acaaaaaaat cctgtctgga
     7861 tccatttcgg tggcggaaat ctttatcgag gatttcatgc agaaatcgct caacgtttag
     7921 cggatctagg cgaattgtcc actggtgtca ttgtttgtga aacattcgat gaagaaataa
     7981 ttgaaagggc ttaccaagat tttgatgatt tgattcttga agtggtcatg ccaagcagtg
     8041 gagagttcgt caaacgtata ttagctgcga cagctgacag tctgtattgt catccagatt
     8101 gttctgaaca ctttgataag atgattcgac actttgaaaa tcctagttta caattagtta
     8161 cttttgctat tactgaaaaa ggttatcgat tgaaagatac aaataataaa tatataggac
     8221 cgattgcagt cgatataaaa aatggtccaa agaagccatc acatacaatg agtattcttg
     8281 ctgcgctact ttataaacgt tttttatctg gagcttggcc gattgcactt gtctctacag
     8341 ataatttttc acaaaacggt agtcaattac gtacttcttt gctaacgatc gcaaaagaat
     8401 ggcaaaagga aggttatgta caaaatacat ttatccaata cttatcgaat gaagaaattg
     8461 ttgcgtttcc atggacgatg attgatcgta tcaccccgaa tccttccttg tcagttagca
     8521 aaatgttggc aaaagagggc ttttcaaata tgtcgatttt ccaaaccgca aaaggaaata
     8581 atattgctcc atttgtcaat acagaggaca tccattattt aatcatggaa gatcattttc
     8641 ccaatggacg tccgaaattc gaaaaagctg gagtattctt gactgatcgt gcaacggtag
     8701 ataaagcaga tacgatgaag gtgacgactt gccttaatcc tttgcatacg tctttagcta
     8761 ttatgggttg tttactgggg tataggagca tagctgagga gatgaaagat ccagatttat
     8821 cgctgctagt ttctgaaatc gggtacaatg aaggcctccc agttgtggag tctccaggta
     8881 tcattgatcc aaagatcttt ctggaagaag taattactgt gcgcttttct aatccaaata
     8941 ttcctgatat gcctcagcga atcgccacgg atacctctca aaaggtggcg attcgttatg
     9001 gtgagacgat taaaaaatat atggaaatag aagttaaaga cccgcagaca cttgtattca
     9061 taccattgac tctcgctgct tggcttcgat accttattgg cattgatgat caaggaaaac
     9121 cattcatccc gagtccagac ccactattga cagactttca gcataagctt tccgatattc
     9181 aactgggtac gcaagataca tcacatattc atgagaaaat ggctcctatt ttggcaaata
     9241 aagaaatttt tggtgtagat ctttaccaag cagagcttgg cagaaaaata gaagaaatgt
     9301 tcatcaagat ggtagcagga ccgaatgccg tacgaaaaac attacaggat tacttacaag
     9361 aacatggagg aaatttatca tggaaatgac ttttagatgg tacggatcac aggaagaaaa
     9421 gattacctta gatgaaatca gacagattcc tgcggtcaaa gggattgtag gcacattgat
     9481 ggatatacca gtaggagagg tatggcctag ggagaggatc aaagaactaa aagaagaaat
     9541 tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc aatgtgcatg atgccattaa
     9601 aattggatta ccttcccgtg agcagtatat cgaaaactat aagcagacaa tccgaaactt
     9661 ggcagaatat ggaatagaag tcatctgcta taattttatg cctgtatttg actgggtgaa
     9721 atctgacctt gactaccgct tagaagacgg ctcatccacg ttagctttta tatctgatga
     9781 tattccagat gatccaagag aaatcgtcgc gcgcatcgaa cattccagca atgaatttga
     9841 attgccggga tgggaacctg agcgcttagc tcatatcaaa tccttgcttg aagcctatgc
     9901 atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat tttctccaat caatcatccc
     9961 aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca gatgatccgc catactcgat
    10021 ttttggatta cctcgaatcg taaagaatcg agaagattta gattggttgt gccgcatagt
    10081 agacagccct tataacggaa tcactctttg tacagggagt attgcagaag atccagaaaa
    10141 tgatgtatat gggattttag cagaattttg tcaaagagat cgaatcccat ttgcacatgt
    10201 tcgaaatatt aaatttttag ctaatcgaga tttctatgag gcaccgcata aatccgaata
    10261 cggttcatta gacatgtacc gcatactaaa aacaatgtat gacaacggat ttaacggata
    10321 catccgtcct gatcatggaa gaatgatctg gaatgagaca ggaagaccag gatacggctt
    10381 atatgatcga gcattaggtg ctgtttatct aaatggatta tgggaagcaa tcgaaaaagg
    10441 aaacaaaagt aaagataatt agtgaaaaaa actgtaattt tattatgtaa ataagaagga
    10501 tgttcaaatt cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc
    10561 ttatttggtc taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc
    10621 ttttaattca aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt
    10681 gatgattcct tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca
    10741 gtcgctgata acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt
    10801 tgaaaaaatt tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata
    10861 gttcacacca atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc
    10921 ttcaaccatc cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg
    10981 ttctctgaaa tccatataaa attgttcaat ggcggtttgg acttctttca acacgctatg
    11041 ttcttgataa agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc
    11101 attataaagt ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat
    11161 aaacaaatca aatacagtac aaaaggactc caccatttct cttgaataaa gaacatgagg
    11221 aacgtttgct ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt
    11281 cgtgatagaa agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag
    11341 attaggtcgc aaaatatcta cagagggatc aataaaaaag tcaatatatt catactctgt
    11401 gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa
    11461 tagaaaatat aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa
    11521 aaaagtattc atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt
    11581 acttgaaaag atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc
    11641 agaaaaagac tttctgccat tattagaaaa ggagatagac aaaccgtaca atgctaaaaa
    11701 tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact
    11761 taaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac
    11821 tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat caattccttc
    11881 aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg
    11941 ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa
    12001 atgatctgct aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat
    12061 atctaatttt gtattgcttt ttttcttcaa gagaaacgtt ttaatcgctt cttgagtttg
    12121 catgaaagag gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc
    12181 ataaatttag acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg
    12241 catacaaaaa aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca
    12301 tttgcagcct taagcttatc gcttactata aatcagacag tccacacaga ggagatagaa
    12361 acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa
    12421 ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa
    12481 gcaaaaattg actattcagg agacttagca cctatactac aatcagctgt tacatttgag
    12541 aatgttgatc ccgctttgtc gattggctat gacgagaaca ctttgactat ccaacacaag
    12601 caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt
    12661 actgtcaagg tatcagatgg actaccgaac gatcatcatc atttaacgcc gtatagtcag
    12721 cggcagacaa ttgagcaaga ttcagctatc gactcaggag gagatcttgt tgaagaacca
    12781 acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga
    12841 gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca accagagcca
    12901 gacaatgaat caaaacctga aataacccct ggagaaaaac cagatgtaga cccagaagaa
    12961 aaaccggatg t
//
LOCUS       DACEOM010000082        12967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEOM010000082 DACEOM010000000
VERSION     DACEOM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.085"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(260..979)
                     /locus_tag="IW781_002447"
     CDS             complement(260..979)
                     /locus_tag="IW781_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1183428.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="IW781_002448"
     CDS             complement(1028..2674)
                     /locus_tag="IW781_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1183429.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="IW781_002449"
     CDS             2853..3443
                     /locus_tag="IW781_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1183430.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="IW781_002450"
     CDS             3535..5040
                     /locus_tag="IW781_002450"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1183431.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IW781_002451"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IW781_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1183432.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="IW781_002452"
     CDS             complement(6476..6994)
                     /locus_tag="IW781_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183433.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="IW781_002453"
     CDS             complement(7010..7336)
                     /locus_tag="IW781_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1183434.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="IW781_002454"
     CDS             complement(7349..9328)
                     /locus_tag="IW781_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1183435.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="IW781_002455"
     CDS             complement(9349..9663)
                     /locus_tag="IW781_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1183436.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="IW781_002456"
     CDS             9831..10124
                     /locus_tag="IW781_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1183437.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="IW781_002457"
     CDS             complement(10202..11314)
                     /locus_tag="IW781_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1183438.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11467..11691
                     /locus_tag="IW781_002458"
     CDS             11467..11691
                     /locus_tag="IW781_002458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183439.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            11701..11880
                     /locus_tag="IW781_002459"
     CDS             11701..11880
                     /locus_tag="IW781_002459"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183440.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="IW781_002460"
     CDS             12074..12244
                     /locus_tag="IW781_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1183441.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>12967)
                     /locus_tag="IW781_002461"
     CDS             complement(12668..>12967)
                     /locus_tag="IW781_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1183442.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4088 a         2608 c         2205 g         4066 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgaaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaggctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg
    12901 catccaattc ataccccgtt ggcgcttgag tttctaccag ctgatatgct cctggtgcta
    12961 agccatc
//
LOCUS       DACEOM010000083        12751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEOM010000083 DACEOM010000000
VERSION     DACEOM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.086"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(172..615)
                     /locus_tag="IW781_002462"
     CDS             complement(172..615)
                     /locus_tag="IW781_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183443.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(631..1791)
                     /locus_tag="IW781_002463"
     CDS             complement(631..1791)
                     /locus_tag="IW781_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1183444.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2298..3998
                     /locus_tag="IW781_002464"
     CDS             2298..3998
                     /locus_tag="IW781_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183445.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(4100..4933)
                     /locus_tag="IW781_002465"
     CDS             complement(4100..4933)
                     /locus_tag="IW781_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1183446.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5237..7402)
                     /locus_tag="IW781_002466"
     CDS             complement(5237..7402)
                     /locus_tag="IW781_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1183447.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7662..9956
                     /locus_tag="IW781_002467"
     CDS             7662..9956
                     /locus_tag="IW781_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1183448.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9949..10614
                     /gene="pgmB"
                     /locus_tag="IW781_002468"
     CDS             9949..10614
                     /gene="pgmB"
                     /locus_tag="IW781_002468"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1183449.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10607..11614
                     /locus_tag="IW781_002469"
     CDS             10607..11614
                     /locus_tag="IW781_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1183450.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11636..12655
                     /locus_tag="IW781_002470"
     CDS             11636..12655
                     /locus_tag="IW781_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1183451.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         3836 a         2681 c         2280 g         3954 t
ORIGIN      
        1 ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt
       61 atcaaagatt gtaacaactg catattcaca cagttaaatt agtgacttca aaatgaaata
      121 atgctcccag caaaaaatta ggctgggagc attatttcgt tattctgttc atcaaaaatc
      181 agcgttttga ttccatttca tcacaagttc ctcctcattt actgcttcat ctttttgctg
      241 tgtttgaatt gtaaaacctt gtgaaagata gaaatgaata gctgattgat ttttttgata
      301 gactgtcaaa gtcagtgttt tattggtttc ttttattttt ttcattagtt gcgtaccgat
      361 tctttgttgc cggtattccc ttttcacaaa gattcctgcg ataaactgat caatcacccc
      421 agcaaaacca ataatttctt gatctatttc tgctaagtaa atcactgcat ctggtaaagc
      481 tttcctaaca gattctactt gattctccca ataagttgat ggaatgaatt catgagaatc
      541 caagttagca tgcagccaaa tcgatagaac agattccagt tctttggcag acaattttgt
      601 tgttgtgcgg atcatttttt ctctccttac ttattcattg attttaaaat aaaaaccatt
      661 tttttgaagc tcttttacaa gttttcgccc ctcaaaaaag tatatcttct cttgttttac
      721 ttttctaata ccaaaagaaa cagcaacttt aggatcatca gctgaaacga ttcgataagt
      781 tccaccaggc aattgatcca gcttgacttt cccatgatgg tcgctttgta ctgaaaacgg
      841 cacaccattt ctagtaagtg tttttttctt atgtttcaaa atgaatgaac taccatttac
      901 cggtttccct gacgaatcac taataataaa tgaaaaatca agcacttttt tacttaatct
      961 atactgatag attagtcgtc cgataaacac taacagtatg actacagcta ttatcgttcc
     1021 tgcaagaata aaccattgcc gcatggttct ttcctgattt ccttttcctt cttctttttt
     1081 taccgattcg ttataaggaa tccgatgtcc tgtcactagc aaacgatgcg tgttgatcat
     1141 atacggcgta catgtaagca aggtaactaa gtcttctcct tctgaaatcg ttagaacaga
     1201 cgtgtcatct ggcagtacca cttgtatatt tttaacttga taagccaatt tctcccccaa
     1261 aacagtcaaa ataaacgtgt ctccgttttc taaacgatct aattcggtaa ataaccgtcg
     1321 attaggtaaa ccgctgtgag cagagatcac tgaatgcgtc gaatttccac caagcgggaa
     1381 agaggttcct tgcaagacgc ctgctccatt ttcaaggatc ctaccattga gccgatcata
     1441 aagtgaaata tttaattgga tcttaggtat attgatactt ccgatcagcc attcctctaa
     1501 ttggctggat gttgtacttt catttctgtt cttttcattg aaaggatcag taccaggatt
     1561 gaatccttgg tcagttaatc gttggttttt ctcctccatt ttctttcgct gcacttcaat
     1621 ttcagctgcg gtccgttttt gtacttgttc gatacgtttt tgatcaataa aactatttaa
     1681 agaatccaca taaaatggat ataaagcgat cagtaaccct acgacgatac atattaataa
     1741 aaacaaacga aacaaaagtc gacgagactg tttttgctta ctctgcttca catgtgtcct
     1801 cccttgcaag tttttttgtt ctttctttta ttatatgggt tcaggaaaaa aatagaaaat
     1861 gaaagccgat gaggttgtga aaaagctgtc ctgcatcaag taataagtac agcccaaaaa
     1921 aatagctact catatttttt gttgggttgg gcttattacc gcagatgcaa gcttttgaac
     1981 accgtttaag gaagaggttg tgaaaactgc gttatgcttt gagtaataag agcgctagtc
     2041 agaaaatagc ttctcatatt ttctaggcta gtgagcttat taccgagaag cagctgattg
     2101 aacaccgttt accgagaggt catgaaaaag ctactttgtt tcgagtatta agaaaaattc
     2161 tgtttcaaaa caaggagatg caacaaaact tatgtcgcat cctctctcct tagaactttc
     2221 acttatgttt ttcttgtaaa attcataaat attttcatga attttctaat ttcttgccta
     2281 cctctcgcat aggatatatg atgaatttat ctaaagtttg caaaggagtt cgacaattga
     2341 aaaaatatgg cccattttta gcaatttttt tcattgcttt ggcatttctc atgcctcatt
     2401 tattcacaca taaactaatt ttgggtgctg atgccccatt ccattacaat cggttctatg
     2461 atgccgcgca acaaattaaa tatggggatt tccattattt tccttccctt tatggattcc
     2521 agcaaagtgg caggatcgtg aatgccgttt acggcccatt tttcgcttat ttacaaggat
     2581 tgatcgtgtt gcttgcaggt agctggtttc attatcagat tctttcaaat ttcattcttt
     2641 atcttatttc aggattttct atgctgattt tactgagata tgtcaatgtt cgaaattggg
     2701 taagtgtacc agtagccgtt ttattcatga gtacgtattc tattcaatac tggtctatca
     2761 accaaggatt cactagctgg gggacttgtt tttatccttt atgtatgatc cctattgctg
     2821 atttcgtcct tcataaaaag ttccctgtta tcaaagtcgc tgtctcagtg ggacttatga
     2881 cacagatcca tttactgtct tcgatcatgt tagctttgat gtatatccct ttttatcttt
     2941 attacttttt taaccagact gaaaaaggaa aagcactgct tcaactgatt aagtcggtct
     3001 tgctttattt tgttctgaca gcaaatattt gggcaggatt agctattgtt tacagtggga
     3061 ataagattct tcctcccttt gttaacagag aaatgagtca aaaggcgata gatctggatg
     3121 gcagttattg gctccattac cctaaggcct ttcctatatt gatattatca ggttttttgc
     3181 tttttatttt ctttcaacaa aagaacacct tattccaaaa aatcgtgttg tacacaagtc
     3241 ttgttttctt ttttctttct actagcttga ttccatggac gacattgatc gaaaaaaatg
     3301 tgccttttat tagtttgatc cagtttccat ttcgattttt cgtgccattt acgctattgc
     3361 ttctacttta ccttgccctt tcactgaacc agtggtctga acaaaagtgg ttccgtcttt
     3421 taagtatatt tggcattctt attttttcag cacagacgat ccagaactta taccagcatc
     3481 tggaaaagtg ggaaaatgaa acatttgtca gtcgacatac ttatctattc gatacgccgg
     3541 aagaagcaag gaaaacattc ttcagcaaag atatgtctac cagcttgttc gttttccaaa
     3601 aaaccacacc tgactatctg ccaatctata aggagacaaa agacaataaa tacgaccggt
     3661 ataacgaaga aatactagaa aacgaaacaa cttttataaa aacacatcca ggcggtcttt
     3721 tgacaatcaa gtggaaaaac actggggaca aaactgttca tatccctgtt atcgtctacg
     3781 atcggactgt gctacagcaa aatggaaaaa cattgactga ttatgaagta acagatatcg
     3841 ggaccccaat cgtgaagcag caaaaaggaa tcaacgagct cacgcttcat tatcaaacgc
     3901 caatctattt ctatttcatt ctttctctta ctttgattgg ctggtttacc ttgctatgtt
     3961 tatttattta tcatagatac aagcttttac gtgcttaggc aaaagggata aatacatccg
     4021 atcaaaaaac tctccaaacg gtataatcgt ttggagagtt ttattttaat cagttttttt
     4081 atgttatatg ttatattttt tattcgttca catctaattc gatttccacc ccgtagtgat
     4141 cactgatgat tggactatcg atcccatcaa aaatccgttg atatttttta acctgcattc
     4201 cttcagggac gaaaataaaa tctatacgca atttttctgt attttgttcc caaccatcta
     4261 ttttcttttc cacagtagct gcttttcctt tttcattcgc gacaacatac gaatcttgaa
     4321 gaagaaggca actttctctt acagtataat aaccttgaga gtctactgga ttattgaaat
     4381 cacctaggaa aaaaagtggt acgcgagttt ccaaaaagta gttttccaag tttttccact
     4441 caaaataaaa gccacttttt ttatctgtcc accaagaaaa gtggcagctc gagacaaaaa
     4501 tgtcctgatc atctatcttt gttttcccaa tcaagatctt gcgtgtctga taatcttctt
     4561 tcttttgaaa ttgggagacg gtaaggacct gactttccaa tgtttttttc gtaagcagtg
     4621 catttccttc ttcatattta tcataaccga tgtggctcat ttcccaactc caaaaatact
     4681 cttttccgtg ttcctttaaa agctgaacta aacggtaggc aaaattatct ttacggatag
     4741 gtacttgatt ctttaccgga cagaacttca ttagcttcgc aggttctact tcttcagatt
     4801 ccagtaattg gttgatttct tgtaaagcaa tcacttcata atcttctaac aggatcttat
     4861 ctgctaattc ttgcaatttt ttctctggtt ctggctcaag ccagctatgc gtgttgagtg
     4921 tcatgaattt cattggtttt ctccttgtct tcatttcgaa acaaaagggt tgtgacaaaa
     4981 tttttgtcac aacctctcca gcaaatagtg ttctcctgct aactcctcag taataagctc
     5041 aatggaccag gagctatttt ttgtttggct gtttttatca cttggtgcag ggcagctttt
     5101 tcacgacctc tccactaaac agtattcaaa ctgctgcttc tcggtaataa gcccaaccaa
     5161 acgaaaaata ggaggagcta tttttgtttg gctgttctta tgacttgatg caggacagct
     5221 ttttcacaac ctctccttac attttaatgg agccgatcac tttaccagct gtcactgtac
     5281 ctaattgctt gatcactact tcttctactt tatcgtttgt aaatacgatc atgatcgttg
     5341 gatctttttc ttcgttttgg atcacatcaa gatccatctc agctaatttc gttcctgttc
     5401 ctacttcttg gccttctttg acagcaatct caaaagctgg ctgattcatt tggactgtat
     5461 cgattcccat atggatcaaa acttcgatac catctttcgt ttccagccca attgcatgtt
     5521 tgctagggaa aatacttgtc acttttccag cgatcggcgc aacaacttct cttgatgttg
     5581 gaataacagc aaagccgtca cccatcattt tttgtgagaa taccggatct tttacttcag
     5641 ataaaggaat cacttctcca gaagctgcgg aaactagttc ttgttctata cctaagtatt
     5701 gcttactacc tgcttgtcca tttcctgtag aaaccatttc tggctctgga atctctgctc
     5761 ctgattgcag cagatcttcg atatctgatt tcaagacatc tgcttttggt ccataaactg
     5821 cttggactcc attgtctttg acgatcaatc ccatcgcccc tgctcgtttc caggcttctt
     5881 cagtccctac tttttctcga tcatttacac ttacgcgtaa ccgagtcata caagcatcga
     5941 catctttgat attttctttt cctcccagca aatgaatgat ctggaccact tgctgatctg
     6001 ctgttcctgt tccgctgtta gatacaacgg tttcttcact gtcattgtcg taatttccgt
     6061 tacgtcccgg tgtagcaaag ttgaattttt tgatcaagaa atttgctaag aaatacgtaa
     6121 caacaccaaa agcgatcaca cacaacacga agttgataag atctccacct agacccgctt
     6181 tgatagctaa cggtgtccga gtcagcaatt cgatattccc aaatgaatgg acccgcaatg
     6241 gcaagatatc tgccatcgca aatgcagccc cttggatcac tgaataaatg acatataatg
     6301 gcacagcagc aaacataaac atgaactcca atggttctgt tacacctgtt agaaagacag
     6361 ccaacgctgc tgaaaaatac attgatttat atcttgcttt cttatcagga tcgacattac
     6421 gatacatcgc aaaagccatt cccatcaaga tacctgaaga accaatcatt tgtcctactt
     6481 tgaaacgagc cggcgtccag ttatgcaaca caaactcata ttgcgacata tcccctgcac
     6541 ctttaagatt cactaaatca gtggcccaag ccaaccataa cggatcttgc ccaaatactt
     6601 gtgtccccgc ctgtgcgcct gataaaatct catacgatcc gccaagctgt gtatagttga
     6661 taggaatcgt cagcatatga tgcaatccaa atggcaatag taaacgttct aatgttccaa
     6721 ataaaaacgg tgctagaatc ggtgcgcttt cttgtgattc tgcaatccat aaaccaaaat
     6781 tattgattcc agcctgtacg tttggccaca ccacagcaag aatcaatgcc acgattgttg
     6841 accataagat aacaacaaac ggaacaaagc gctttccatt gaaaaatgac aaagcatccg
     6901 gcaattttct ataattatag tacttattat aagccattgc cccgacaaat ccagcgatga
     6961 tccctacgaa tactcccata ttcaaagctg gtgcttccaa tacacttgta aaaaatcctt
     7021 taaccatgat cttcgtaccg aacagagtgt gggtaaatgc atcttcattc gcaagcatgt
     7081 cacttgtgac accaaagata gccccagtga tccgattgat taaaataaat gagatcccag
     7141 ctgcaaatgc tccgcctgct ctttcttttg cccaacttcc tcctattgct aaagcaaata
     7201 aaatatgaag attaccaata attgcccaac caatattttc aattactccg cccgttgtga
     7261 caagcggcgt ccaatcagga ttgatcaagg gaattgattt ccctatactg atcatcaacc
     7321 cagctgccgg cataactgct accacaacca ttaaagcctt tccaaatttt tgccaaaact
     7381 caaaactaaa tatctttttc attccgttta gctcctttac gcaatcggtt tcgttaaacg
     7441 tattatagcg tgcgttttct tttttgtaaa tgctttattt tttatttttc tgtctatacc
     7501 attctataaa acctataata cgtgtgttcc cttctataat tttctcctaa aaatttattt
     7561 ttttaagctt ttttctatga aatattttct tttgtctatt gacttgtgca atcggttgcg
     7621 tttattatga acgtataaaa cagctcatta ggaggagaaa aatgaaacag atcaaacgac
     7681 tatttgctat tgatccatgg aaaatcagca cgacacaatt agataaaaaa aatcttcgac
     7741 tgcaagaatc tttaacaagt attggaaacg gttatatggg gatgcgcggg aactttgaag
     7801 aaacttactc aggggaccac catcaaggta cttatttggc gggtgtctgg tatcccgata
     7861 agacacgtgt cggctggtgg aaaaatggtt atccagaata ctttggaaaa gtgatcaacg
     7921 ccttgaactt tatcgcgata gatatttcga tcaacgatca gctgatcgac ttggcttctt
     7981 tggaaccaga agacttttac tgggaattag atatgaaaaa tggtgtatta tctcgtacat
     8041 tcacaatcac tacttctact aataaagtac gcatttcctt cgaacgtttt ctgagcattg
     8101 tcaaaaaaga atcagcttat atctatttaa ctatagaaat gcttgaaggt agcggaacag
     8161 tcaaactcgt atcaaaattg gatggaaatg ttcaaaacga agacagcaac tatgatgaac
     8221 atttttggaa tgagattgat cgtggaacta tagaagaaat cggctatttg acgactaaaa
     8281 ctattccaaa caactttggg attgaacaat ttactgttac tgcagcaatg cgccatttcg
     8341 ttaatggaaa atcagtcatc cctgactatc acgaagatac tttagcatta catggttgct
     8401 tgtctgcaga attatctcaa ggtgagacat ggtcagtgga aaaagaaatt atcgttctta
     8461 ctagtagaga tattccagaa gcgcagcaag cagaaaaagc tgtctcagaa ttaacgaacc
     8521 agacaaacaa ttatatccaa gcaaaagaag agcaaacagc ggcttgggca gaacgatgga
     8581 agcttgccga tgtggtcatc gaaggagacg aagaagcaca acaaggtatt cgattcaatt
     8641 tgttccaact attctctact tactacggcg aagatgaacg attgaatatc ggccccaaag
     8701 gattcacagg agaaaaatac ggcggagcca cttactggga tacagaagcg tatgctgtcc
     8761 ctctttatct tgctttagcc aaacctgaag taactaagaa tctgctgaaa tatcggcata
     8821 atcaactgcc gcaagcaatc cacaacgctc agcaacaagg attaaaaggc gcgctttatc
     8881 ctatggtcac cttcactggt gtcgaatgcc ataacgagtg ggaaatcaca ttcgaggaga
     8941 ttcaccgaaa tggcgcgatt gcctatgcaa tctacaatta tacgaattac accggcgata
     9001 cggaatattt gaaaaatgaa ggactggaag ttttagcaga aatcgcacgc ttctgggcag
     9061 accgtgtcca tttttcaaaa cgagcaaata aatacatgat ccacggtgtg accggcccaa
     9121 acgaatatga gaacaatatc aataacaatt ggtatacaaa tacgatcgca gcttgggtct
     9181 tgcgttacac gcgagaaaat tatttaactt atcaaaaaga agctgctatc actatcagcg
     9241 aagaagaact agccaaatgg aaagatatcg cagagaacat gtacttccca gttgacgaag
     9301 aattaggaat ctttgtccag catgacacat tcttagataa agatctcatg cctgtatccg
     9361 atattcccac aagcgatctt cctctgaatc agcattggtc ttgggacaaa atccttagat
     9421 cttgcttcat caagcaggca gatgtactac aggggattta tttcttcaat gatactttta
     9481 caaaagaaga aaagcaaaaa aactttgatt tctatgaacc aatgactgtc catgagtctt
     9541 ccctttcgcc aagtatccat tcgattttag cagcagaatt aggaatggaa agcaaagctg
     9601 tagaaatgta ccagcgaaca gctcgcttgg atttggataa ttacaataac gacacagaag
     9661 acggcttgca catcacttcc atgactggta gctggttgtc gattgtccaa ggatttgctc
     9721 aaatgaaaac tgcccatgat cagttgagat tcgcaccatt tctgccaaaa acttggaaag
     9781 cttattcttt ccatatcaat tatcgcggac gtttattgtt catctcagtc acagatgaac
     9841 aagttacttt cgagttatta gaaggttctc ctctagaaat ttacgtctat gaacagtctt
     9901 attctttagc ggatcgtcta gaaataacgc taagaaagga ggaagcaaat gtttaaaggg
     9961 gtattgtttg atttagacgg tgtcattacc gatactgctg aattccatta tcgtgcttgg
    10021 cgaaagcttg gtcaggaaat cggcattacg atcgaccaca cattcaacga acagctaaaa
    10081 ggcgtcagcc gagaagactc actctctttg cttttagctt atggcggaaa agaacactct
    10141 ttttcaaagg aagaatttgc agaattagcg aaaagaaaaa acgattatta tttagagatg
    10201 atccagacga tcgaaccaaa agatgtcttc cccggggtcg tgcctttact ggattcttta
    10261 aaagaagaaa aaatcaagat tgctcttgct tctgcaagta aaaacgggcc attcctactt
    10321 gaaaaaatgg gtcttacacc ttactttgat gctattgccg acccggcaga agcaacaaac
    10381 ggaaaacctg cgccagatat cttcctcctt gcagcaaaag ctgtcggact aaaagcagaa
    10441 gaatgtatcg ggatcgaaga cgcgcaagcc ggtatccaag ccatcctctc aagtggcgct
    10501 caaccaattg gcgtgggacg tgctgaagac cttggagagg agatcccgct tgtccctgat
    10561 acgacttttc ttacgatcga ttacttgaaa gaaaagtggc atgaccatgg ataatccaga
    10621 gatctccgtc gtaccagttc cagagactga aataaagaaa atcacattga agaacgacta
    10681 cttagaagtc gttcttctca attacggtgc ccgcctgcac caaatcttcg caccagacaa
    10741 agaaggaaaa tcagaaaatg ttttattgtc ttacgatcgt ttcgaagatg ttctaacaga
    10801 caaaagcttt tttggagcaa ctgtcggacc tgtagccgga aggatccggg atgcttcttg
    10861 gggtgagcat agattggaga aaaactgcgg atcacaccat atccacggtg gcacgaaagg
    10921 ctggtctttc caattttggg aagtagaagt tttcaaaact ccatcatcaa taggtgtggt
    10981 cttttactta aaagatgaat tttctaccta tccaggacca atcactgcta cgatcactta
    11041 ccgtttaaca aaaaacgaat tagagatgac aacctctgct agtagccttg ttgaaaccat
    11101 ctgcaatccg accaaccatg cctacttcaa cctttcagga aatggaacaa gagacatcta
    11161 tgaacatcag ttaacggtct ttttagatgg tgtgcttgaa ttagatcaag aaaaactgcc
    11221 tactggaaat tggacaaaaa aagaaaaact gccaatcgac tttagaaaat ccccaacact
    11281 gcaagagatc cttgcatgtt atccagatgg actagatgat gtatttcttt tgcatcatcc
    11341 taggctttcc aaaacttcct tacaattatt cgagaagcat tctggtcgcc aaatgactat
    11401 agcaacttct aataaaagta tggtattatt ttctacgact ggattcgaag ctgatttctc
    11461 tgtcaatgga aaacagatgc attcaaacta cggattagca atcgaaccgc aagaaatccc
    11521 tgatatcgtt catttcccca aatttggatc gatcaacctc catcctggtc aagaacggat
    11581 cagtcagacg atttatcggt tctctgcaca ataacaagag aaaggtgttt ttatcatggc
    11641 tattaccgta aaagatgtgg cgaaaaaagc cggtgttgcc acctcgaccg tctctcgtgt
    11701 catcaacgat catcctagta tttccgagac aacaaagaag aaagtccgaa aaattatgga
    11761 tgaactaggt tatgttccta acatgactgc ccgcaatcta ggaaaacgca tttccagtgc
    11821 catcggcgta atcttacccc ctcttgattc aaaagagcgg atcggtaacc cattttatct
    11881 tgagatcatg gaagcaatca acgaagaagc gcggaattat gatatgacta cagcaatcgc
    11941 tactgctaaa aattttgaat tgctgttaga gaacgtccag cgtatgcatt tacaaaaaca
    12001 agtagatggt tttattctag tgtattcaga tagtcaagat actgtcattg attatttata
    12061 ccaaaaccac atccctttca ctttgatcgg acagccttat catcatgaga acgaaatcgt
    12121 ttatgtagat aatgataacc agcttctagg taaacaagca acagaatttt tgatccagaa
    12181 tggacataag aatatcctgt ttgtgacaaa cacgactcat gaaagtttgt atttcgaacg
    12241 atatttcggt tatcaaaaag caatgatgat ggctggtctt cctgcgttat ccagtgtagt
    12301 actagagcat ccggaggatt atctgtcctt tgagaaaacg ttgaaagaaa acgaagccac
    12361 agcattggtg gtcattgacg atatcttcgc ccttcgcacg atgcagttgg cgcaaatgta
    12421 tggctatgtc attccagaga cactctccgt cattagcttc aacaactcga tcttctcaac
    12481 attaacacac ccttacctta caagtatcga tatcgatatc tccgaattag gacgtgtcgc
    12541 tatgcaaaag ctgcgcgaat tgatccatga agaattgagc agtggcgtac gtttggtcat
    12601 ccctcatcgc ttgatcaaaa gagagacagt cgttccttta gaaaacccat actaaatagc
    12661 tgactcacta aatacgctga ccatttatcc tatggtcagc tttttatgta aaagaagata
    12721 ctacttctaa cacattgttt atctgtaaaa a
//
LOCUS       DACEOM010000084        12584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEOM010000084 DACEOM010000000
VERSION     DACEOM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.087"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..2376
                     /locus_tag="IW781_002471"
     CDS             4..2376
                     /locus_tag="IW781_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183452.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     VPTDFLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VREIEATPEDKKRIESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3906
                     /locus_tag="IW781_002472"
     CDS             2437..3906
                     /locus_tag="IW781_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ1183453.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3917..5926
                     /gene="topB"
                     /locus_tag="IW781_002473"
     CDS             3917..5926
                     /gene="topB"
                     /locus_tag="IW781_002473"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1183454.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6272..6868
                     /locus_tag="IW781_002474"
     CDS             6272..6868
                     /locus_tag="IW781_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1183455.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6881..7780
                     /locus_tag="IW781_002475"
     CDS             6881..7780
                     /locus_tag="IW781_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1183456.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7783..7914
                     /gene="ssb"
                     /locus_tag="IW781_002476"
     CDS             7783..7914
                     /gene="ssb"
                     /locus_tag="IW781_002476"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1183457.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7936..8268
                     /gene="ssb"
                     /locus_tag="IW781_002477"
     CDS             7936..8268
                     /gene="ssb"
                     /locus_tag="IW781_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1183458.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8290..8547
                     /locus_tag="IW781_002478"
     CDS             8290..8547
                     /locus_tag="IW781_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183459.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8706..8828)
                     /locus_tag="IW781_002479"
     CDS             complement(8706..8828)
                     /locus_tag="IW781_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1183460.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9018..9242
                     /locus_tag="IW781_002480"
     CDS             9018..9242
                     /locus_tag="IW781_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183461.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            9685..9891
                     /locus_tag="IW781_002481"
     CDS             9685..9891
                     /locus_tag="IW781_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1183462.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9891..10142
                     /locus_tag="IW781_002482"
     CDS             9891..10142
                     /locus_tag="IW781_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183463.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            10323..10595
                     /locus_tag="IW781_002483"
     CDS             10323..10595
                     /locus_tag="IW781_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183464.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            11040..11219
                     /locus_tag="IW781_002484"
     CDS             11040..11219
                     /locus_tag="IW781_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183465.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11278..11634
                     /locus_tag="IW781_002485"
     CDS             11278..11634
                     /locus_tag="IW781_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183466.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         4641 a         2066 c         2617 g         3260 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgta
      661 ccaactgatt ttttgaagga gaaagaaaac gatcattttt atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcatactca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaatagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaaactta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gttggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccacagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 tcattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gctgaactaa agccgaaaca tgaacggcaa aattttttag ttggagacga ccgtttagat
     2941 catgcggttg cttttttatg gaaagatcca cagacaggag agacagtagg ggcaagttat
     3001 caaggaacga tcgtcgattt taatcgtttc ggtaaacggg gaacgtataa acacattgac
     3061 aagaatccta cgccaaatca cggatttaat ttaaaaatag gcgatccgaa gcatttaaaa
     3121 ttctttgaaa gtagtattga tctgttaagt tacgctgcat tgaatcggga gaaactacaa
     3181 gatgcctggt tagtttcgat ggacgggtta aaacatcatg taatcagtca ttacgttgaa
     3241 gagtcgattt ctgaattaag acgaaaacaa acatttcctc aatcgattga aatatgtgtg
     3301 gacaatgatc gtgcaggaca tattttttat gaaaaagaac agatgaaggg aatcgttgat
     3361 ccttttacca ataagaaaat ccgttgtgag cgaggaattc caaatgattg gcaagtacct
     3421 aaagaataca aagctactta tgaagcagtc gcaaaagaaa tgagtgttga acctgaagca
     3481 attatggcca tacacaaaac tgaaactaat ttacagctga caaatcaatt ggtgtctgct
     3541 catgatgttc aatctacgtt tggaaagatg ttggcgaagg gcgaaccagt agaaacaatt
     3601 gatttgaaag aagcatgtac cacggtcgcg aaggaactga aagtatgtga acgcgcagat
     3661 ggaacataca actttgatcg tttttatagt agaaaagcca acataaaaga cgttaatgca
     3721 ggaatccttc tttcctataa agcagaacaa tactacaaag gctataaaaa gcatgaacat
     3781 gaatttgtac ctgaagtgaa aaaggattgg aatgatcaat tgaagcatga gatccagcaa
     3841 caagaaatca gaaagcaaaa acgtgcgatg ttgtttcaac aaggcagaca acaagaaagg
     3901 gagtgaagtc gtcactatga aaaccgtcat tttagcagaa aaaccgtcac aagcgaaggc
     3961 ttatgccgat agcttttcta aagctacccg aaaagatggg tattttgaga tccaagatcg
     4021 attgtttaca ggagaaactg tgatcacgta tggttttggt catttagtcg agttagattc
     4081 gcccgatatg tacgatgaaa aatggaaaca atgggcatta gaacatttgc ctattttccc
     4141 caatcaatac cactatcatg ttcctaaaga taagaaaaag cagtttagtg tcgtcaaacg
     4201 acagctgcag tcagctgata cgattgtgat cgcgactgat agcgatcgtg aaggggagct
     4261 gattgcctgg tcaatcattc aacaagcagg cgccaatcaa ggaaaaacct ttaaaagact
     4321 ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccaacagt tacgagacgc
     4381 agaagagacc tatccaaaat ttgaagaagt acaagcacgt cagatcgccg attggttaat
     4441 tgggatgaac ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag
     4501 tttttcttta ggtagagtcc aaaccccaac actctatatg atttatcaat tgcaggaaaa
     4561 gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gcccaagtaa tcgctcaaaa
     4621 tggcgcattt gatgcgaagc ttgatccaaa tgaaacccaa gcgacacaag aagcatttga
     4681 agcctattta aaagaaaaag gtgttcaatt aggaaaacag ccaggtacga tccttcaagt
     4741 cgaaacagag aaaaaaagtg cggctagccc acgtcttttt tctttatcga gccttcagtc
     4801 taaaatgaat caactcatga aagctagtgc caaagatact ttggaagcca tgcaagggtt
     4861 gtatgaagga aaatatctaa gctatccgcg aacggatacc ccttatatta cggaagggga
     4921 atatgcgtat ctgttggatc acttagacga atacaagcat tttttaaaag ccgaagcgat
     4981 tccgactcct attcatacac cgaatagtcg gtatgtgaat aataagaaag tccaggaaca
     5041 ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac aactaagtcc
     5101 actgcagcag gctatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa
     5161 gtatacctat gaagaaacaa cgatcttgac gcaagttcag cagcttcagt taaaggcaat
     5221 cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga gcgacggaaa
     5281 agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg tgacggtgga
     5341 tctacaagtt cttgaaaaag aaaccaagcc accacaacct tataccgaag gtacgttgat
     5401 cactgcaatg aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat ccatattgaa
     5461 agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa ccttgaaaca
     5521 aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag ggattttgct
     5581 ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg ccaaatggga
     5641 aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc tagccaatat
     5701 ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat ccactgattt
     5761 tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg cgcgtcattt
     5821 aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg tggcagcttg
     5881 tacgtctgaa gcctgtgatt ttaaaaggtc agaagggttc atttgaagca acgattgttt
     5941 taaaagaaga cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga
     6001 aaagaacacg ccgcactgca aaataatgga gggttaatct tatgcgtaat accacacaca
     6061 aacaacaaga aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg
     6121 ccagcaaacg aagactgcag caattgaaga accagcagag gattcaaaaa gaaatcgaat
     6181 tagatcgata aaatcttcct aactattgta tacgtataaa cttatgttat actgttttta
     6241 taagtttata cgtatacaaa ggagggagaa aatgacaaaa gcaatggtat acaagctgct
     6301 agaagaattt aatggaactt tggctttgaa ggatgccaaa aaagcgggca tttctcctgt
     6361 aacgatcaaa cgtatggtag atcgagggga gctggatcga gagtatccag gttttttcac
     6421 cttacctggt caatttcctg atgaattgtt tatggcgcaa aaaaaatatg aacgcggcat
     6481 tatttcacat attaccgcgt tagatttgta tgatttaaca gatatgatcc ctagacagat
     6541 cgatctgact gtaccttacg gttatcacgt ttctgaaaaa ggattgaagg aattcgcagt
     6601 tgagctacac tatacaaaag cggaatggta tgaattaggt aagattgaaa taaaaagtcg
     6661 ctatgggaat cctattatag cttatgatcc agaaagaact ttatgtgaca tttggaatcc
     6721 ctggtatacc gttgaggatg aaatcaaagt aaaagcaatc aaaaactata tggagtcgga
     6781 cagaaaaaat cttagaaaat tgaatgagta tcgacgaatt ttaccaacag acaaaacgat
     6841 gcgttcttat attatggctt taaattaaag gagaagatac atgacaccta cgcagttaaa
     6901 agcaaaagtg aagaatattt ctagagaaaa agaagttgat ccgcagcttg ttttaagaca
     6961 ttttatgatg gaaaaattct tggaaaaaat ctcagagtct ccttatcgtg aaaattttgt
     7021 gttaaaaggc ggcttcttga ttggctcaaa gtatggcatt gaaaatcgca caaccaaaga
     7081 tattgatacg actttacggg aaatgaaagt aaccaaagaa acattaacaa cggttctaaa
     7141 cgaaattttt tcaacgccga cgaaagaagg cattcaattt gagattcaag gaatgaaaga
     7201 aacgagagaa gccgattact atccaggatt tagcttacgt gtgctggcac atttggaaaa
     7261 catgcggcca gattttaagg tagatgtgac tacaggtgac tcgatctatc ctgcaacgat
     7321 cactcacagt cataaattga tgtttgagga tcgaacgatt cctttggaat cctatcctac
     7381 agaacaaatt attgctgaaa aattaagtgc aacctttgat tttttaacag ataattcgag
     7441 aggaaaagat ttttatgatc tctatactat tcctaaaatg gagaagatcg atgagaagac
     7501 cttgaaagat agtgtgagaa atacatttac acgtcgagga aaaactgatc cgttaaagat
     7561 atactatgaa aaggatatgg atgtactgaa aaatagtcac gagctaaaag atacctggaa
     7621 gaaatatcaa aaaagcaatc cttatgctgc gtcgatcacg tacgatgaga caatggattc
     7681 gattagtaat ttgatggaaa aagtgattca cgtcgaagaa aaagagcgaa aactagctta
     7741 tcaaagacac caacagatga atagagaaat ggagagataa agatgattaa taatgtgaca
     7801 ttagtaggac gcttaaccaa agatcctgat ttacgttata ccgcaagtgg tactgcagta
     7861 gcgactttta ccctagccgt caaccgaaat ttcacgaatc aaaacggcaa ttgagaagcc
     7921 gattttatta actgcgtgat ttggcgcaaa ccagcagaaa caatggcgac attagctaaa
     7981 aaaggtagct tgattggtgt agttgggcgg attcaaacgc ggacttacga caaccagcaa
     8041 ggacaacgtg tatatgtgac tgaagttgta gcagataact ttcaactatt agaaagtaaa
     8101 gcagcgacag agagccgtgc gcacgctgat caatcgtcca ccagtccaag caccgccacc
     8161 tttgaacaac ggaatacagc gacgcctaac aacaatggat tgaacgcttc acagaatcca
     8221 tttggtggac aatcaatcga tattagcgat gatgatctac cgttttagtt tgagaaagaa
     8281 ggagtaagca tggcaaaaaa aattagtctg acgatggatc tattgatacc cgtaattgca
     8341 tttatagacg ggatttcttt gggcgtcact gaaaatcctt tggtaaatag aattgcgaaa
     8401 ggatcactat ttcttgtatt cgtgtatctt gtctgttctt atcttttaaa aagagcagga
     8461 aaggataatt ccttttggcg attgactaaa gtcacagtag cagcgacagc tgttatactt
     8521 acggctattc taccgcttta tatgtagagt taacgggaaa tcgttgaact tcttgcgaaa
     8581 tattgctata atgaacatga aaaagaggag ttgcgctaac aacccctcat taacagcacc
     8641 gattaaagaa cggtggcaga atattagtta tttggaaata accgctgacc tgctaaagta
     8701 gacggttatt tctttttgtc atttttaagc atatttacaa tcaaactaat caaagcaatg
     8761 gtaaacatac caaaaccaag aatggtttga attgtctcat atgcggacaa aaggcttact
     8821 cctttcatag attttagcac ttacattcat aagcaccacc acctttccag aagtcgccac
     8881 cgtcttcaaa ctttactgtc ttgactatta tagcatatct aaaatcgaat aaaaattctc
     8941 aattttagct gtttttgcta taattttagc gaggtgatcg tgatggaaca atatattggt
     9001 ttactcgata aattagggtg atcaagacac agccattgct tgtgcgattt acattacgca
     9061 cgagtcatga accaatcaat tgtgtagtgg ccaatataga aatagcggat aaattgctga
     9121 ttttggaaga aggcaagtat actatttcag tgacaggtca cttcaataaa cgaaagcaac
     9181 tagtcattga atcaatgcaa attagaaacc ctgatcattt tacgcgatcg atgagcatat
     9241 aaaagaagag ctgcagtcct aaatgaggat tgtagctctt tttgttttac atagaaacag
     9301 taaaaatgaa aaggcctgtt taggaaggca caagcgcctt ctcaattgaa agacctttct
     9361 ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgctacg cgttcactaa
     9421 gataaagtgt cttgcatagc aagacaaaat agaaaaaatt tcctctcacc aaaaggttcg
     9481 ctcctgttcg ttatctcttt attgatttgt cagaccggca acaagttgcc ttttatcacc
     9541 aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcttg tcgcttttcg
     9601 ttgattcatt ctggccaaca aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt
     9661 tcctaagagg aggcagaatc aatcatgcga aagaaatttg attattgggg tgttcccttc
     9721 agcgaattat tcccaaacta tcatgcacca catactgtcg aatgcgattg tggagaaaga
     9781 gcgaaatgta tcaaaagcta ttgtttgtat caatgtccaa catgcgggaa aaaatatacc
     9841 ttgtcttatg gcgattatat tttaattgat gagaaaggaa agaaacgctg atgaaaacac
     9901 aacacaaatt attgactgta aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt
     9961 ctgaaaaagt agcagaagtt ttacttattc cgcggtactg gacgatagaa acccctttag
    10021 tagaggatgc accgactatg ctatttgaag acctagtgaa gattgcagga tatgaactgc
    10081 attatcaaga aaacttggaa acttatgaaa acgaatattg gttaaccaaa ttagagaact
    10141 aaaaaggaga aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg
    10201 ataaaatggc acaaaaaatg gaggatatga cgttcctata caacgaaaca cgtgttccaa
    10261 aaaaacatta taaagaaaaa ttgtcggtag ctgtggaaga aatgattgaa taaggtgtcg
    10321 aaatgaatct tattgcgacg tactatcgaa cactggaaga actgaaaaag caaaatgcaa
    10381 aatggttttt ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actattaaac
    10441 cttctgaata ccaagcttta gaactaacgt atgcgaaatt tatcgaaacg aaaaaagcca
    10501 agactgtttc aagtgaatgg ttagattatt ttgaaaatat caataagtat ggcgcatatt
    10561 acaccatgaa aaaggaagac aacgaaaatg aataaggtga tttatcaact aacaacggat
    10621 tccatatcaa cgatttgaaa gaaaaaaagg tcaataatca tttataccga aaagtgagaa
    10681 tgtgtgaaac aaggacaaaa aaagcctagt aactaaggaa atggttctta tttcgcacca
    10741 atcgctccaa ccgcccccgc cgcttaggtg gtgcgaaata aacgggcaaa gcccgataac
    10801 ttaagagaaa gaaggtgatc catttgaaca agattattcc cttattgtta gtactgtttg
    10861 ttggcgaaat acttatgcga gatagtgcag ctgtacagtt aaacctcgct gtatttttgg
    10921 taatactggt gttttgtttg atcttttggc ggtttgtatt agtcctcgtg agtctactat
    10981 tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag tgagtaccaa
    11041 tgccaattgc aataggaaat aaacggttac ccgtgacatt agatgaaaag agacaaaaag
    11101 aattgcagca actaaagcag aagtatggca aaagtgaatc taagattatg tgtattgcgt
    11161 tagatctatt gatcgcgcaa gaaaaagcag gatttgatgt accagcgcta aaaaagtgac
    11221 gtcacttttt aatttgatct atataagtga tatcactttt accaaggagg gaattaaatg
    11281 ttaaaaacta aaaagaattc gtacaaatta aagcttagta tactatctat tatttgtctt
    11341 ttaggcatga taattagtgg tcatttgtat agagtggcat atacgcagtt tattgagtac
    11401 aaggttcact ctttaggatt agctggggat cgatacgttg aatatgtcca ttattcatta
    11461 aaatgttctt catatattct tctttggctt attttttcat tgtttgaatt gctaaattta
    11521 atagcaatat cttcatatca cttagataag ctaaatacca aatctagccg ttataacgaa
    11581 gtgtttaagg aaaatgtgaa gttagaaagt gacttaaaca agctaaaaaa ataaaaagaa
    11641 gaaaacataa gttaactaaa attattttat taagaatgat ggaatataag gataaggtta
    11701 ccaacataac gatcattact tctctgatat gctatggact tctatcaatc agtattttgt
    11761 ctatagatta tggagtaact atactgcaag ccttagtttt ggctatgcct atgtatctat
    11821 ctttgtttat catgcatgaa atatataagc tatttgtttt atcaaaaaat gtagaaatac
    11881 gggtaaatat tttggtgaat agttatcaaa aagaactcaa agaaagaaga cggtggcggt
    11941 atttattgaa aggatgaaaa agtaggattt gacatactgg tattcagaaa gtgccgtcac
    12001 ttttaaaaca atggaaaagg tggagataag atgaagaaaa aacaaactaa actatcatta
    12061 gcagatattt aacacagtta accgcaactg aagatggtgt tgttgaattt gaacaatctg
    12121 aaattgcggt agtagatgat cgttacctta aaatgcctta cttctttgac caagcaaagg
    12181 tcatttgcct ttttcactac gatggggtac gtgattttag gcgttaaaaa gtagtgatta
    12241 tatcaacttt acgagtaaat ataaatttag acatgaaact gaattatcca atggaaaatc
    12301 atggtttata atctagaaaa attatagtaa tgattggatt atatatattc tttcgtattt
    12361 tcgtacaact gtagcttgtc aaaattgaga aatttaaccg attaggtact ttacatcgtt
    12421 atttgggtaa tttctaaaac atgaagctta ccaacgaata aactgagact atcttattaa
    12481 ttgatcaata taagaaaata tctttgagga gatgatgtac tatgttttcg tctatccacg
    12541 attggttagg tggtcttttc ggcggtggcg gtaaaggttt ttaa
//
LOCUS       DACEOM010000085        12462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEOM010000085 DACEOM010000000
VERSION     DACEOM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.088"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(483..944)
                     /locus_tag="IW781_002486"
     CDS             complement(483..944)
                     /locus_tag="IW781_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183467.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            1077..1451
                     /locus_tag="IW781_002487"
     CDS             1077..1451
                     /locus_tag="IW781_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1183468.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            1505..3631
                     /locus_tag="IW781_002488"
     CDS             1505..3631
                     /locus_tag="IW781_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183469.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            3628..4248
                     /locus_tag="IW781_002489"
     CDS             3628..4248
                     /locus_tag="IW781_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183470.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            4941..5321
                     /locus_tag="IW781_002490"
     CDS             4941..5321
                     /locus_tag="IW781_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1183471.1"
                     /translation="MSEQNQNLASNYSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            5303..6217
                     /locus_tag="IW781_002491"
     CDS             5303..6217
                     /locus_tag="IW781_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1183472.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            6462..7151
                     /locus_tag="IW781_002492"
     CDS             6462..7151
                     /locus_tag="IW781_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183473.1"
                     /translation="MAFYLPYLLIFASILGIIGGGYKAYQTRYSLKGSRIRFKRFLLL
                     GVVFTLSIVSSGLFGVLEGNKRVSRSVLLENVTQKYEVAHEKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(7271..7357)
                     /locus_tag="IW781_002493"
     CDS             complement(7271..7357)
                     /locus_tag="IW781_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1183474.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            8003..8935
                     /locus_tag="IW781_002494"
     CDS             8003..8935
                     /locus_tag="IW781_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1183475.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            8952..9422
                     /locus_tag="IW781_002495"
     CDS             8952..9422
                     /locus_tag="IW781_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1183476.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQAVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            9506..9823
                     /locus_tag="IW781_002496"
     CDS             9506..9823
                     /locus_tag="IW781_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183477.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRI
                     LLF"
     gene            10113..11294
                     /locus_tag="IW781_002497"
     CDS             10113..11294
                     /locus_tag="IW781_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1183478.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNAKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            11384..11653
                     /locus_tag="IW781_002498"
     CDS             11384..11653
                     /locus_tag="IW781_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1183479.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYK
                     KYPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWTASSRTAKYYYYI"
BASE COUNT         4723 a         1869 c         1896 g         3974 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttagattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaaataa tgagagttta ttcttgaaaa agcaacgcgc
      241 ttataaagcg ctaccaaaat tttatcatat tttataaaaa ataacaatcc agtaaaaaat
      301 gactggtaaa aattcacaaa gaaacggaaa gaagcaacta cagaaaatca agccttaaaa
      361 agagcttttt acctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga
      421 ggggcaactt ggtctagtcg acattatgtc aactatgcaa ttaaataact aactacttca
      481 tattagctta ataaaataat caaaatcact aggggaaaaa atattgtata aaaaaatttt
      541 tcatttttta aataagaata gtaaatataa aaataattta agatatctat tactattaat
      601 ccagatagag caaaccaagt aaatattccg tgtgtaaagc taaaaaaatt catttgaatc
      661 aattcaaata aacctaatat agtaacaaat atattaaaaa taatagctgt caataccagt
      721 aaattatttt gtttattttt taaattatct tcatttaaaa tagaatttat gttagtacta
      781 taaaattcac aaattttttt tagtgtctct atatctggtg ttgttttgtt attttcccac
      841 tttgaaatag tttgacgtgt aacattaaat tttcttgcca catattcttg agtataccct
      901 tttttcactc ttaattctct tagcttagat cctaaatcct ccattttttc tcctatgtaa
      961 acgattattt acataaaatt ttatcataaa aatactaacg cgccaaaaaa gttctttatt
     1021 ttataaaaca cactaaaata aaataattct agtaaaaaag ataggagaaa taagttatga
     1081 aaaaattttt atgtctttct gctttaatgg gtgtcttatt aactagtgga ggaatagtta
     1141 gtgctacaga agctttaaat ctagatgtac ccgaagacca taccgccatt tacggaggag
     1201 gaatggaagc tacagaagaa ggattttctt ctagtaaatt gagatatgct gctggcggcg
     1261 gagatttcaa ctgtggggtt aatggattta aagtttatgc aaattactac catgctagag
     1321 ctaaacatag tgcaacagct aaaaatggac gaggcggtca agtacgatca gttcagaaag
     1381 ctggagtaag agcttatgca acatgcaatg ccacacttac tggaaatact ggttggtgga
     1441 atgtttatta aaaaagagag tttaaactct cttttttatt tacatttgaa aggaaattca
     1501 aaatatgtta aaaaaaataa acgctgtttt atcacttttt gtctgcatta caatactatt
     1561 cttttctact aatgttataa atggtataaa gacttctgaa gcgctatata aaaataccgc
     1621 tgaaattcat atgcaatata aagatgatat ggactttaac agttatataa acgctttgta
     1681 tgaaatatct acacaagaaa atattaatat ttctcaatat agttttactg ctgttaacca
     1741 actaactatt cttgctacaa accctaacgc taacaaacac tggaaatttt ctaaaaaaaa
     1801 tatattagca agaaacgatt ctaaagtcca ttatactaat aacaaaaatt ctaagtacca
     1861 attaaaactt ccaaataatt ttttaaatat aaatatttat cctttttctc atgtggaaaa
     1921 tattggatta agtgaaatat tatatgtaca aggaaactca aacaaattaa ttcctgtact
     1981 gaaaaaatat ggaacaccta ctatttccaa tatcaatcaa tcagatactt ttcaaattaa
     2041 tactaatatt gttcttgtta tctgctatct tagtatattt attgtaatta ctacaattat
     2101 atttgctttt tcaaaaatga aagaaatcac tctaaaaaaa atgttaggct ataactcttt
     2161 tgatactata aaagattcta ttaaagagtt aaacctctta ttttgttctt tgattataac
     2221 tttcatttta tctatttttt tgagcatata ttacagtaac gaaaattttt tcctatcatt
     2281 tcttctatgt agtatttttc attttataat tagtttttta tttatcatag ttactctaag
     2341 tatctatcat ttatctagta taaaaaatta tctaaaaaat tcacgaccac ttaagttaaa
     2401 tctttgtata ttaaatatct gtctattctt aagcatgatc cttctgttaa tagcttctac
     2461 aaaagtaata aatatccata atgaagccga aaataattct cttaaatatt gggaaagaac
     2521 tactaattta tataaaacaa atattactaa tcaattaaat agaaacaaca ccgtagaaga
     2581 aaataactac ttgaaaaaag ctagtaaatt tgtttataaa atacagaaga attataaaac
     2641 ttttattatc gcgccataca attacgcaac tatccaagaa aataataaag aattttttat
     2701 tggacaaaaa gtttatccaa attttgaaga ctatgttagt caacctgctg gtgcaggaat
     2761 atgtgttgat ttaaactatc taaaatatta caatcctatt tcttttgaag aatctactga
     2821 cttaaattta ataaactctg atccactaac tacttacatt ctagtaccta aaaaatataa
     2881 agaatatgca aaaatgattg aaaaaaatta tttagaagac atacagttta gacttgaaga
     2941 ctctcctcct caagctcctt ataaaactcc taatttgaaa aatttaaaaa taaaactaat
     3001 atttgttaat aataaccaaa agtattttag ttttaatacc ttatatggaa aggcaaaaga
     3061 taactataca ataactgatc ccatagttag agttattaat ccagaaataa cagaatcatt
     3121 attttgggga aatatattga ctagtgatgg tggattattt tttgatcaga aacatacttc
     3181 aaaccaaaac ttttttaatc aaattaatcc gtatataaaa gaatttaacc tagaaaatat
     3241 cataaatttt tcttattctg tttttcaaga aactggtgaa ttattatctc aaaaaaaagt
     3301 aaataaatta aatgcatatc tacaatatct tttaatatta ggattattta taataattaa
     3361 ttttcaaatg atttttgtta gttttactat aaattcaaaa aaaatagctt taaaaaaaat
     3421 atttggatat tcaccaataa gtcaagtttt agatttatgt ttactccctt ttacaataga
     3481 gttatttgct actatgtgtt ttcaattgat attttcaaaa aacacttact tgtattttat
     3541 aatttttatg atctttttaa taaatctaat tacttctatt attatttata aaaaaaatag
     3601 ttttaaatat tttaaggaga atattttatg atattatcag ttgagaattt caccctgtca
     3661 attaataaaa aaaagatcaa acaaaattgg aacttttcaa tatcagaaaa agattttatt
     3721 atagtaacag gaaaaagtgg tagtggaaaa actactctgt taaataattt aagcctaata
     3781 gataaacaat ataccggtaa cttattctat tttgaaaaga aagtttctaa acatgatata
     3841 caacgtctac gaagaaatca tattagctat ttatttcaaa actatgctct tcttgaaaat
     3901 caaacagtac aatacaattt cgaattagca aaaaagttta acagtaatat ttccaatgat
     3961 catatttata atttattaac agatttttct ttgccaaaag atatcttaaa agaaaaaatc
     4021 tttctcttaa gcggtggaga acaacaaaga atagcattaa ttagaaatat gttaaaacca
     4081 tttgatatat tatttgcaga tgaacctaca ggaaatttag ataaagaaaa ctcaactttt
     4141 ataatgaact ttctgaaaaa tttaacagaa aaagaaaaaa aagccgttgt actagtaaca
     4201 catgacttaa atgttctaaa ctatggaact aatattatag aattataatt tttacccgtg
     4261 atgctagctg atttaaaact agcaccacgg gtatttattt tatattccat ctctcctatc
     4321 ccaaaattta aataatcact taataaaaag gaggcaccca agtgatttta ttgaaaaaag
     4381 agtatgaaaa attttatgat agacttattt attctttgca attgcccaaa aaattaaacc
     4441 gtcaattaca aaagacttat gaaactaaat caactgaaga atgggagagt tattgctaga
     4501 tttttggtag aagtaacaaa aagtggacat attatcataa tgactcgccg aaagtctgat
     4561 aaaaaattgg ctaaaaatat tgctgaatgt ttaataaaac tatcagtttt agctaaatag
     4621 ttagtagttt acacaaaaaa gcatttatgt tttttgttgc aaagcaacaa tttcatcttc
     4681 ggtgaaataa gcgaaacttt acaaaaagtg gctagcattt gctagctttt tttgtattgt
     4741 ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac aagcggttat
     4801 ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac ccttttagtg
     4861 agctttactc attgtacctt tttaggtaca atggtattgc gaacgatacg tccgtaggac
     4921 gtactgaaag ggtgggactt atgagcgaac aaaatcaaaa tttggctagc aattattcta
     4981 aaaaatatac ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc gaatccgaat
     5041 ttgcaaagct gaagcaatcg gctgaagctt tgcaaatgag tgtgccggct ttcgtgaaag
     5101 ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt gcccaggcaa
     5161 tcgcgcgcga tttagccaaa gctggaggga atatcaatca aattgctaag tggtgcaata
     5221 cacaccaaca tgacgttgct tctggtgacg ctcaacgttt atccgaaaac ctaaaaatca
     5281 tgcaaaagga gctacaaaag atatggcaac aattaaggta agccgaacca cttcttgtag
     5341 tcgtgcaatt aattatgcag aaccacgagc aaccattaaa acaggaataa actgtgacgt
     5401 cgattatgcc aaaagtgaaa tgaaacaaat tcgcatgctg tatggaaaag acgaccgtgt
     5461 acaggcacac ttattgattc aatcttttcg accaggagaa attaccgctg aaaaagctaa
     5521 tcaactaggg gcagagtatg caaaaaaaat agcccccgat caccaaatag ctatttacac
     5581 acatacagac aaagaccata ttcataatca cattgtcatt aactccgtta atctggagac
     5641 tggaaaaaaa tttcaagctc atggtcaagc atttctaaac aaatgttatg acctaaatga
     5701 cgagatttgt cttgcacata acttaccaat cactgagcga ggaaagaagc cagaaaaacg
     5761 aaccatgtct gaaataaaat taaaagaaaa aaatgaacct gtttggaaag acgaaattcg
     5821 gattgcgatc gatcaaacaa tgaaaaacaa aaaaaccaga acctatgacc aattttgtaa
     5881 ttccctcaaa acattgggga ttcattgctt caatcgcggg aaaaatttca cttacgaatt
     5941 gatccacaaa aagaaaaaag tacgatccaa cagactagga aaggactacg aaaaggagac
     6001 gattttacat gagctggaca gacgagaacg aacctataac caacagcgaa ccaacgagct
     6061 acgaagagct cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag aacaacgaac
     6121 tcttacgcct gaacaaccgg aaattagacc ggctcgaaga aaccatgaac aacacgttga
     6181 aagaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac atagaaaacg
     6241 aaataaaaag acaacgtgct tttcttgcgc cttataccca agaaaggata caaactacaa
     6301 ttgattcaca agtcaaaaaa ctccaccaag cgctattaga ccagcgaaac caagaacaaa
     6361 aaaaggcttt aattcaacga tcgatggata ttcttgcccc cttatgttgc ttaatcatga
     6421 cactgttcgc cttttggttg gtatggggag aataaaaaga catggcattc tatttaccct
     6481 atctactcat ttttgccagt attttaggaa ttattggagg tggctataag gcttatcaaa
     6541 caagatattc attaaaaggc tctagaattc gatttaagcg ttttttatta ctaggggttg
     6601 tatttacctt atcaatcgtt tcgagcggat tattcggcgt tttagagggc aataagcgcg
     6661 tttctagaag tgttttatta gaaaatgtca ctcaaaagta tgaagtagca catgagaaaa
     6721 agaaaaaaga gcaagtatta gcacaaaaaa tagaaaaatt taccgcttgt tatgaggaca
     6781 tgaatgatat ttttgtgaag caagaaaagc gtttgacgga taaaaatatg gaaacactca
     6841 ctcgacttta tcggaatttg ccagaagaac agcaaaaaga gtaccaagaa aaatatgaac
     6901 aagtaaaaaa agacgtgcaa tatgtgaaag atacgaaagt ggaagaagct tgtagtgatt
     6961 tatttggaga tacaattcct ttttcaacta gcgaacaaga acgaaaagaa cgacaacaat
     7021 cagtcacata tgaacgctat gaaatcttgc ttcaacaagc aattaacata caaaatctga
     7081 ctaaaaaaga aacagcttta aattatttaa aaagcgtcaa ggaatggtta gatcagcaac
     7141 agcaaaattg aatttgattg aaattcatgt taaactaaac atgcaaatgt tcaaacatta
     7201 gatacacgaa agccccagag aggtcggatt ctctggggct ttttttaggt tgatgtcgcc
     7261 tttactgatt ttacttcttg tttcggttat tcaaccaata actgaataca gagatacaaa
     7321 ctcctacaag aagggggcaa ataaacagtt caaacatcag atacacctcc ttccagtcgc
     7381 aagactgtcg gataggcgac aagttattat accctacaaa attaaatctg tgtggcaatt
     7441 ttaagtatct cttatttccg ggttgttagg gcttgccctg accgcctgta gggcgcttta
     7501 aattccagat aaaggttttt atactaagac tgtcttaaaa cggcttagac aagctcttag
     7561 agcctcaacc ctcctttttg aacaatggtt ttagcaaagg agctggaggc gactgcgcag
     7621 gtataatgtg agagcgtagc gatcacacct ttttggcgat cgtagggagc gaggttttga
     7681 attagcagtt tagaaaagtt cgcgaacctg tttaaagaaa aaataattgt ttttttaggt
     7741 gggaaatttt gtctccctct tttataatct ttttttaaac ctctttttaa acctctttac
     7801 cgtcggctgt gtccttactc ccccaaggga tacaaaattt tataggcatg aaattatctg
     7861 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     7921 tatctgtttt aggtattgtt tttagacata atatgtccta aaatgatgtc aataaaatca
     7981 tttagactgg agtaacaact aaatggcaaa tgaaatcgta aagtatgaac cagaattaaa
     8041 taccattcct ttaagaaaat ttaccccaat agaaatgaac ttgttttttt ctattgtctc
     8101 tagaatgcgc gataaaggta atcaaaaaat acgcttttat ttcagccaac ttaaagattt
     8161 aagtgcgtat aagcagactg caggagaagt ctttataaaa gatttggcaa gaacatacga
     8221 tcatttgatg gatttacgtt tcggaagaaa aagcaatggt ggattaacaa tagaacgatt
     8281 tacaatgttt actgattttg aaattcatgg agaagtcgat aacccttatg tagatataaa
     8341 agtccacgaa aaagctttgc ctttacttaa taatctagaa agctgggtac gtttctcttt
     8401 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tgctaaaaca
     8461 ataccgaact caaggatggg ctgaattttc taaggaagat tttttagagc tattagatat
     8521 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccaa taaaagaaga
     8581 actaactcca ctatttaaag gattaactat tcgtaaaaaa tatggtaaag gtcgaggtaa
     8641 acctgttatt ggttatcgtt ttacttggaa agctgaaatc aaccatgctg acgatttttc
     8701 aaaagggaag caagaagatt tacgtataaa attatttaat attgaacaca atggagaatt
     8761 aacacaaaaa gaaaaatggc gtgctaaaga tcgaatacta aatcttccgt taggtactca
     8821 tgaagctgat tttaaaaaac aacaacaaac cgaaaaagaa gaagcggaaa aacaatcaat
     8881 ctcaaacgag cttaaacaag atttattaga aaacttacaa aacttattcg attgaagcat
     8941 aaggagtatt tatgatagat aaaacgatca aagaactcgc ggaagaattt catgtttcaa
     9001 aacaagcaat taggaaaagg ctatcagctg attttagaga aaactatgtg caagctgtta
     9061 ctaggaacgg agtgaaaacc ttagttgtca gctacaaagg atatttgctt ttaaaacaac
     9121 attttttagg tagtaacacc actggtaacc aagtagaaac cgttgctagt aacgatgaaa
     9181 actcgattat tgttttttta catgaacagc tccaagaaaa agatactcaa ataaaacaaa
     9241 tgcaaaagtt actcgaccag caacaacaac tcaccttgca ggcaaatcaa cagatcgaaa
     9301 aactgcaaga acagttacaa ttagcttata cggaagaacc tgaagaagag gaattaactt
     9361 ctgtcaataa agaaaacaac aatgcaataa caacagaaaa aaagtggtgg aaattttggt
     9421 aacaattacc aactttccat aaagaagaaa tcaatttcta tagactctat ctttctaaga
     9481 ataaaaaata tgtttaggtg atcaaatgac tataagtaaa gtaatcaaac aaaaaagaat
     9541 agaaaaacaa ctaactcaag aagatttagc tgaaatgctt ttagtatcta aaaaaaccat
     9601 ttctaattgg gaaaatggtc gaacaatacc agatacagaa aatttaattc agtttgctaa
     9661 gttatttgat ttatcactag acaatatttt gttaggagaa gaaaaaatta tggtaaaaaa
     9721 ttatggtaaa aaatcataca aaaacactaa tactattatt tcattttgta atttttttaa
     9781 gtttagtttt caccttaatt cagtccggat tcttttattt tagccattca attgccactt
     9841 ggttattaat attttttcta agtttactat taataattaa tacattaata attaatactt
     9901 taattattat ctttaataaa catagaaact tatttcttca cagcatattt ccaattgttt
     9961 tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat aaaaaagtag
    10021 ctaattgtaa ttttcgtcta aaactcaaaa ctagtaaaaa taactttatt gtgttatttt
    10081 tatttataaa ttatgaaaaa ggacctttcg aaatgaaaaa taatagaatc tacaaaaatc
    10141 agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga ttactttctt
    10201 tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa gaattaatta
    10261 aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat ttttttaatc
    10321 ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata atgttattac
    10381 ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa aattggtttt
    10441 atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac acccttatgc
    10501 tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatgcg aaagatgatt
    10561 ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta ttattttttc
    10621 agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt ttgtttacgt
    10681 ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt ttatccttaa
    10741 tatttttata tttggttata tcagatacag gaagagattt cttattaaaa ataggatttc
    10801 acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat ccaaatggat
    10861 ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt ttaggtactg
    10921 gttttaatgt atcaaacatt cacgttcctg ttagagaatc cgatatgata ttttctgtaa
    10981 ttggagaaaa ttttggcttt ataggaagtt gtcttgtaat attcctatac ttttatttaa
    11041 tttatcaaat gtataaaaca tgttttaaaa gcaataactt atttttcacc tatatcggta
    11101 taggcattgt tgcaatgatt ttttttcatg tttttgaaaa tataggtgca agtatcggat
    11161 tactacctct aactggtatt ccactcccct atattagtca aggtggttct gccattctta
    11221 gtaatttttt ggggttggga tttatgttgt gttgtagtag atatactaac gaaaaaaaag
    11281 gaacacaaaa atgaattcaa aataacactt tatgaataga atgtctattt caataaaaat
    11341 tatgatacct taaagttaat cattaaaaaa ggagtgtcat aatttgaaaa aaacaaaatt
    11401 attggtagct agtttgtgtt tgtttagcag tttattagct ttcacccctt ctgtttcatt
    11461 ttctcaaaat ggtggagtgg ttgaagctgc tgctcaaaga ggatatatct acaaaaaata
    11521 tccaaaaggt gctaaagtgc caaataaagt taaaatgtta gtaaatatta gaggtaaaca
    11581 aactatgcgt acttgttacc ttatgagttg gactgctagt agtagaacag ccaaatatta
    11641 ttactacatt tagtaaaaac aaaacattct ttctatacaa taaaatagcg tatagagagg
    11701 atgtttttaa tttgaaaagg agtggttatc tttatgaaaa atagatcatt tgctatatta
    11761 gtattatccg taattttatt agatatattg ttaataacat tagctttaaa aactttaaat
    11821 ttagtaacaa gttttgtcat tataccagta attataggaa tgtctattta tttcatcaaa
    11881 aaaaataaac aatttttttg aatgaaatta cgggcgaaaa taatcactca aaattattaa
    11941 taaaaacgaa tcaactactt tttctgaaga aacatacaac cgtaaacaac aattgttgtt
    12001 tacggttgta aattatcaaa ctatatggtg acttgttgct taaaataggt tgaaatttcg
    12061 tttatttttt ttgagaaatt ttacccacgt actacccatg tacctatcca cgtattaccc
    12121 atgtaccccc catgacaaat gggcggaggg gcttgcggcg cttggtatta aaggcattat
    12181 ccgcctccca ggtttctgac cattttctct caacgcggta aaaaccccac ttatgagaga
    12241 aaatggtcag aaacctaaac gatcggcaaa gaaaacatac caagcgccac aagcaaaaaa
    12301 agttagagta acttccagaa aagaaaaatt tttagaacaa cttttgaaaa tttgattttg
    12361 atttatatcc gttcaattta aacatttgat aaactgaagg ctagtattta aaatgtgtat
    12421 acaagtggta gcgttaaaaa aagtggaggt ataacctatg tt
//
LOCUS       DACEOM010000086        12335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEOM010000086 DACEOM010000000
VERSION     DACEOM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.089"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(119..481)
                     /locus_tag="IW781_002499"
     CDS             complement(119..481)
                     /locus_tag="IW781_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1183480.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(492..1613)
                     /locus_tag="IW781_002500"
     CDS             complement(492..1613)
                     /locus_tag="IW781_002500"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1183481.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(1629..1979)
                     /locus_tag="IW781_002501"
     CDS             complement(1629..1979)
                     /locus_tag="IW781_002501"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1183482.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(2109..3620)
                     /locus_tag="IW781_002502"
     CDS             complement(2109..3620)
                     /locus_tag="IW781_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1183483.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(3942..5294)
                     /locus_tag="IW781_002503"
     CDS             complement(3942..5294)
                     /locus_tag="IW781_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1183484.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(5447..6523)
                     /locus_tag="IW781_002504"
     CDS             complement(5447..6523)
                     /locus_tag="IW781_002504"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1183485.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            6719..8464
                     /locus_tag="IW781_002505"
     CDS             6719..8464
                     /locus_tag="IW781_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1183486.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(8744..9181)
                     /locus_tag="IW781_002506"
     CDS             complement(8744..9181)
                     /locus_tag="IW781_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1183487.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(9165..10538)
                     /locus_tag="IW781_002507"
     CDS             complement(9165..10538)
                     /locus_tag="IW781_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183488.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(10860..11348)
                     /locus_tag="IW781_002508"
     CDS             complement(10860..11348)
                     /locus_tag="IW781_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183489.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(11370..11972)
                     /locus_tag="IW781_002509"
     CDS             complement(11370..11972)
                     /locus_tag="IW781_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1183490.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            12071..>12335
                     /locus_tag="IW781_002510"
     CDS             12071..>12335
                     /locus_tag="IW781_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1183491.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIG"
BASE COUNT         3568 a         2653 c         2230 g         3884 t
ORIGIN      
        1 aaaaaagata cgttcgcttc ttctagcaga aatgctggta ctagaagcag acgtatcttt
       61 ttttacgcac agctggtcct tttttatatg taaaggctgc atggttactt cgctatattt
      121 atttagtaac aggagtcagt gcttcgatac ccacactaat tttcaatgct cggtcaactt
      181 ctgccataat gggtcgatcc aaatggcaga ccttttcttt caatcttgat ttatcgatcg
      241 ttctgatctg ctccaacagg ataacggagt ctctttctat tcctgttcca gtagctttga
      301 tcccaatgtg agtcggaagc ttaggtttag ccatttttgc cgttatggcc gcaacaatga
      361 tcgtcgggct aaaatggtta cctagattat tttgaataac caaaactggg cgtgttccgc
      421 cttgctccga tcccacgact ggagaaagat cagcaaaata aatatctcct ctttttacca
      481 taggatcctc cttattcttg atattctctt ggtatccgct gtcctaatcc gcaagccact
      541 tcatagtgga tcgtacctaa ttgatctgct atatcctgca tagtgatttc tttgtctgca
      601 tttttgccga taagcgtcac ttttgttccg acaggaaatt cttgaggcaa tcggatcatc
      661 agttgatcca tacatacacg cccaatcgtt tcacagtagt tcccttctac caatagagaa
      721 aaaccctgca tctttcgtgg ccagccatct gcataaccga tcggtatcgt tccgatccac
      781 tctgcttcag gagtgatata tgtttctcca tagccgattc cttcacctgc tgggagtttt
      841 ttgacttgaa tcaattcaga aaccagctca agtgctggct gcaatggata aacttccggt
      901 agtgcgtgcc cagagggatt aagtccgtac attgctactc cataacgaat catattgccg
      961 attgtttcat catgccataa agccgttgca ctattgctga catgtacata tcgaggaagt
     1021 tcgggaagtt ttttcagcac ttctataaag cgttcttttt gcagattcca atatgtatca
     1081 tctgcctgat ctgctgtaga aaaatgggta aataaacctt cccattctaa tacacgactt
     1141 tctttgatga atgcagctgc ctcttttact tcttcaggtg tacaaaatcc gatacgaccc
     1201 atcccagtgt ctgcttttag atgaatcgaa agaggcgtct ctagcttact tttctctaaa
     1261 acagcagctg ctgcttgcaa ccattcccgt gtacctgcag ttactgataa atcatgagca
     1321 acagctacag tcagtgcgtc gtaaggaacc atattcaaga taaggatggg ctgagtaaat
     1381 ccagcttctc tcaactcgac tccttcatct aaatttgaaa cacaaaagcc cgaagctcca
     1441 cctgcaactg ctgcctcagc tgtttcaata gctccatgac cgtatccgtt cgcttttact
     1501 actgcaaaca attcttttcc ttgcggaagt cttgcaactt catttgctac attttcagtt
     1561 atagcttttt tatgtatcac tgctttcgtt gggcggtgcc aagcgacaac catcgttttt
     1621 ccttctttct agtcttccaa aataatttgt gcaaaagctg tttcatttgt atgagtaata
     1681 ctgacccata cattgccttc atgaggtgaa cgcgtcacaa caggctgacc attttcattt
     1741 tttaaaatct cgacatcttg aaaggtaact tttccgatcc cagttcccca agctttagag
     1801 aaagcctctt tgcaagcgta acgcccgcct aaaaattcta cttggcgatg aaaaggcaag
     1861 gactggaata gtttcatttc atcagaggtc aagattcgtg caatgaattt tggtttttct
     1921 tcgatcagcc gagtgatcct aggaagttcg actgcatcga tcccaattcc ttttatcata
     1981 tgcttttctc ttttcaataa atttagaaca ctctcattct accaaattat aaccattttg
     2041 ccacaaataa gtccacaaat aacaaaaatg aaagtttctc ttaaaaaaga gaaactttca
     2101 ttttatgttt aatcgttatt tgaacggatt acaaatccgc gttttttatc attgtgacgt
     2161 ttaccgccat ctttttggct gttattattt ttgctataac cgccttttga tttgtttcca
     2221 cgataattac cgccgtcacg acgattacgg ttgtttccgc caccacgttt gccattttta
     2281 ttgtatcctt ttttctgcat tggcaatggg cgttctggtg tgatcttcac tggcactgca
     2341 tcagctggat cttttgctgt cgtttttagc aaaagtgcaa ctaaatcttg tgcagaatat
     2401 tcttctaata atttatctgc tgtttgcagg tatttatcta gaccattttc tgcaagtttt
     2461 gtttcgattt gttcaactgc agcacctaat tgacctttga atgcttcttt ttcggttggt
     2521 ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa tcacatgcaa gtagtccatt
     2581 tcatttggtg taacgaaagt cacagacata ccgccttttc cggcacggcc tgtacgtccg
     2641 atacggtgaa cgtagctttc tggatcttga gggatatcat agttgtacac atgagtgaca
     2701 cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta caaggatatc taaatgaccg
     2761 ctcttaaatg aacgcaatac gctcattcgt ttttgttgag acaaatcgcc atggattcct
     2821 tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt cgtctacacg gcgttttgta
     2881 cgtccgaaga cgattgttaa ttcaggtgtt tgaacatcaa acaagcgagt catgatatcg
     2941 aatttttcgt attcttttgc gcggacatag tattgatcga tcaagtcagc tgtcatttct
     3001 ttcgctttga tcttcacatg atgtggattt ttcatgaatt taacgccaat attcttgata
     3061 gctggcggca tcgttgctga gaaaagtaat gtttgtcttt gatcaggaac ttgtgaaatg
     3121 attttttcga tatcttccaa gaagcccata ttcaacattt catctgcttc atccaaaact
     3181 aatgtttgaa cggttccaag tttcaatgtg tggcggttaa tgtggtcaag catacgtcct
     3241 ggtgtaccca caacgatatg tggacgatct ttcaagccac ggatctgacg gccaatatct
     3301 gctccgccgt aaacggcttg tacacgtact tttttgtcgc gccctaaacg atataattct
     3361 tcttgtgttt ggatagctaa ttcacgtgtt ggtgcgataa ctaaaccttg taattcgtga
     3421 cgatctggat cgattttttc taacattggt aatccaaatg cagctgtttt ccctgtacct
     3481 gtctgtgctt gtccaataac gtcttttcca gctaatgcta gtggaatcgt ttctgcttgg
     3541 ataggcgttg cttcttcaaa accagcgcgt tcgactgatt tcaacaattc tggtgataat
     3601 tctagttctt taaatttcaa atgaatcctc ctatatggtt gttaactgag gatgtagctt
     3661 ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt ttctttttga cagtggccac
     3721 gcacgttttt atccattctt agcccctgtg gcaccaccga aaaatcactt tgcgaagttt
     3781 aggcattaga aaagccaaac gggtcgcgtt cagctttatc attattatga catctggctt
     3841 caacaagttg tttctatggc aaaaccagtc caatttctct tcatctttcg gcaataggtt
     3901 attctaacat agtttttcgt tttctgcaag tttacagatt tttatttcat ttgctgaagt
     3961 tgttctacca cttcaaataa gcccatgctg ttgctgcctt ttagcataat actatctgtt
     4021 gcttcaagat tttgtttgat agcatcaatc aatttattct tttctttttc caaatagaac
     4081 acaggtagtt gggggtattt ttggttcaat tcttctttta gtgcatgcat ttgttcccca
     4141 taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt cactcatttg cgcatgcata
     4201 tttaaagact ctttccctaa ttccagcata tctgctaaca cagcgattct gcgtcctttt
     4261 gttggcagat ttttaaaagt atctaatacc agtcccatgg ctgttggatt tgcattgtag
     4321 acatcgctca aaatagctgc accatttcct gccgttagcc attcggttct gttttgagtg
     4381 atagaaacat gggcgagccc ttggcgtatc tcaggtatgg acagtccaaa gaagcgcccg
     4441 atcgtatacg caatcaaagc attcgttaca ttataacttc caggcagcgg gatcgtaaat
     4501 gtttctcctt ctactacaaa atcggtttgt gcttttcctt ttgctgtcac ttccgcatga
     4561 acatcgccat ttccaatacc gaatgttgtg actgtttgta ctaatgttgc cgttaaaggt
     4621 gtcagcaacg gttcatctgc gggcacaacg agtaatccgt ctgatacaag tccgtcagtg
     4681 atttccattt tggctttagc gattccttct cgcgacccaa gattttctat gtgtgcttca
     4741 ccgatcattg tgattgctgc agcttttggt tgagccataa gtgaaagttc cgtgatttca
     4801 ttggcgtgat ccatgcccat ttccaaaacg atcttttctg tatcttgggg catgtgcaaa
     4861 atcgtgtaag gaagaccaat attattgttg taatttcctt gcgtcttata tgttttgtat
     4921 ttttcagaca agacagacgc ggttagatct ttcgtggtgg ttttaccatt gcttcctgta
     4981 attgcaacga cttctgcgcc tactttttcc aagtaatagg ttgccaattg ctgcatggct
     5041 ttcaaaggat ctgctacagg aataactgga aaattttttg gagcttgttc aacaggcaag
     5101 ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct ggataaattc atgtccatct
     5161 cgcgtaccag caagaggaac gaataaactt ccttcggtta tcttccggct gtcaaattcg
     5221 acattggtaa tctttgtcag tgcagagcat tcgctgccca cggcttgtgc aatttcttgt
     5281 attgttagat tcattgagat ctctcctaaa aaaggttgtg aaaaaagcgt ttggcctaaa
     5341 cggcgggttc gcatgactct cacttccaag taaatttctc tattcgtatt tagaagctag
     5401 aatgaacgcg gttacgcaat cactccagcc tcttctttat ttctctttat tcattttttt
     5461 caaaaaaaga ttgacgctga tggtatcgat tcattcctaa ctggatcagt tcttcaatca
     5521 aatcaccgta tttcaagccc atattttccc ataagagtgg gtacatactg aactccgtaa
     5581 atcctggcat agagtttaat tcattcagga ataattcatt tttatttgtc aaaaagaaat
     5641 cgcaccggct caatccgctt ccacctaaca tcgtgtaagc taacttcgcg tactcttgcg
     5701 ctttttgata aacttcttct ggcacttcgg ctggaatctg catttcgatt ttattattga
     5761 tatattttgc ttcataatca tagaatgcta cgtcttttac gacttcacca ggcaaagtcg
     5821 tccgaacgtc ttcatttcct aatacagcaa cttcgatttc gcgcgcttca attccttgtt
     5881 caacgattgc tcgagaatca tactgatagg ctgttgctaa agcattttgc agctcttctc
     5941 ggttttctgc ttttgtaatg ccgacactag aacccatatt cgccggtttg acaaacatcg
     6001 gataaagcaa agaaccttca cattgatcaa atactttttt aggattttct ttccattgat
     6061 tcttaagtac tggtacataa ggaacttgcg gcacaccagc agcttgtaaa atatacttgg
     6121 tcatgatttt atccatggca catgcactgg tcaatacgcc tgcgccgaca taaggcatat
     6181 tcaatgtctc taagaagcct tggatcgttc catcttcccc gtttggccca tgtaaaactg
     6241 gaaaaacgat ggctccttct tctttgattt cgcccggatt gatcactttt cctgtaaagc
     6301 cttcctctgt ctgtccactt gggtcccatg aaagatgcaa gacatctttg ctggcaggtt
     6361 tttctgttag taatggacct ttgacccatt gtccttcttt tgtaataaat acgagttgaa
     6421 cttggtaata attataataa atggcattta aaactgaaaa tgcggaaaga atggacactt
     6481 catgctctgc gctgcgtccg ccatatagta aagtaatctt caaaagattg tcctcctgta
     6541 ttctaaatcg ttcagtgatt tactctcata ttttagcaca aaacaaaaat aaacgaataa
     6601 cttatttata aaaaaacgag ccagcttcta aaagaacgag cgtcatttat tttttgtcag
     6661 ctagattatt tgtcccgttt tttataaaaa gttatgataa agaaaaggag ggatgatgat
     6721 ggcgcaaaca aatccacgca gccgcttggc agaaatcatt cgagtgttca tcaattacaa
     6781 tgtagtgcca aactttgtac agcaaaaaaa tcccgaacag gtaaaaaaag cctttgaaga
     6841 actaggcccc accttcatca agataggaca gatgctttct gttcgagaag atttgctttc
     6901 ttctgccttc actcaaacat tcaaaacatt acaagatagt gtacctagcg acacattttc
     6961 aacggtcaaa aagacgatcg aaacagaact ttctctttca ttatcagata tattcgatga
     7021 tttttcaaaa tcgccttttg cctctgcttc tatggggcag gctcatcgtg ccaagctgaa
     7081 gaacggtgac gcggtagtcg ttaagatcca acaccctaac atcgccgaag agatccgact
     7141 tgatcttcaa ctatttgaac gagcgatccc tttgatcaaa tacattcctg aaactagcgt
     7201 agttgatcta aaaggggtac tccaagaagt aaaacgttcc ttgatcaatg aaatggactt
     7261 cttaaaagaa agtcaaaatg gcgaacaatt ctatcaaaag aacaatggct ggaaagagat
     7321 ccgcagcccc aagatttatg acgctttttg ttcgaaaaaa gtgatcgtga tggaagaaat
     7381 gtctggtaaa aacttgaatc atttaatgaa cgccgaaaac aaaactgaaa cttttatcac
     7441 tggaattcaa aacaagcagc tgaaacaaga agttgcaaag cttttagttg agaactttat
     7501 gaaacaggtg tttgatgatg gttttttcca tgctgatcct catccaggga atctgctatt
     7561 ccatgtacta acaaaagaag aacagacaca agctagccga aaaactgaaa cagtccatga
     7621 aaaagagttc ggttcctttg cttttcgagc ctccacttct gcagaagacc ctgttgcgcc
     7681 atataccatc aactacatcg attttggtat gatggggcat ctttctgcag gacttaggca
     7741 aaaactcaca caagccgttt tagcgctgta tacgaaagat gcttatcgaa tcgaaaaagc
     7801 ggtccttcga ctttgtcagc aagaaggctc gtttgatgaa agtcggttcc accaagaact
     7861 gacaagtttt cttgaacagt attatgattc tcctattgat gaaatcaact tgcaagaagt
     7921 attcacacat gtcgtgacga tttgtcatca aaataatctg cagttcgatc gtgatatcac
     7981 gcttttgctg aaagcatttg ggacattaga aggggtgatc cgcgtcctcg atcctgaagt
     8041 ttccctcatg gaagtcgctt cgccttttgc tcaacattat tttctcactc atctggatgt
     8101 agaagataca ttgaaacaaa gcggattaga cttattggaa gggatgaagg ctgcgcctaa
     8161 gatccctcaa cagctccacc atctattaga aatgtggact tcgggacaag gaaaagtcaa
     8221 tttggaattg aaaaagcaag acaaattatt gagcaggatc gaaagtatga tcaatcggct
     8281 tgttttcggt atgattttag ctgcattgat cgtagggtcc tccctgcttg tccaagcagc
     8341 acctgtagaa aacgcagagg tcgtttcctt attagggata tttacgtatg ctattgccgc
     8401 attcgtcatt atttttttgg caatcgatgc attgatccaa atgtataaga agaggaaaaa
     8461 gtagaaaaag gttgtgacag aaagaatcag cttcaagaaa taagaagggg ttttacataa
     8521 attgtttctc aaatttttgt gaaaattcgg cttatttttc gaagaggcta cttctgaaac
     8581 accgagaata aaaaatgtga aaaagctgtt ctgcacccag taataagaac aatcaaaaag
     8641 ggaacatgga acacatttat cctgtttccc tgttcccttt ttgatgtatt cgagcataaa
     8701 taaaatacct tcgttaccta ctatcttctg tacttggtaa accttagtca attgcctttt
     8761 ttaacgcttg acgaaaagac atatgatctt tctccgtaaa gggttttgga ccctttgtcc
     8821 ttatgccagc ttttcttaac tttccactat gataacgttg ttctagaagc gtgccgatat
     8881 tcgcttcgtc aaattctttt ccagagtgat ggaacactcg cacacccttc ggcaatacaa
     8941 gtgaagctaa accataatct tgcgtgatca aaaagtcatt cttcttaacc ataccgacga
     9001 ttttataatc cgcactatca gctccgcggt ctacgtagac gatttggaca tgattagcgt
     9061 attctttggt cgtataatgg tccacactgg tcacgataac cacgtcaaga ccataggatt
     9121 ctcctaattt tatgacatct tcttttactg gagaaccatc tccatcaatg aatagcctca
     9181 ttctttttcg ccttcttcca taaatgataa acagttacta atccgctcag taaaagacca
     9241 acattgatcc attttgccaa gataagcagt tgtacacttt ctatcggcac tgattcatgg
     9301 ttcagcgtga tcaataatag atggattggc tctcccattt cttttgctaa aacaaacaaa
     9361 agcgtcatca ataaaaactg gtggatcaca ctccctttat tttgagtagt atacttgaca
     9421 acgatccggc gatccattgg aatagctgta tcagtttgat aatattcctt atttagccag
     9481 ctgcctgtta ctacgtgcca gaaactaagc agaaagacac ctatcgcaaa accatttgga
     9541 atcaagagga ccaataaaga agaactcagt ccaagcaatt gcatagccaa tggagaccat
     9601 gttaaacgtt tcaaaatcca tggtccgact atttttgctg caatcaagcc tgctgcataa
     9661 ggcaaataca taaaaatcga aagtttttct aatccgtaca gtatgccaac ataaaaactg
     9721 ctaaataaaa aaaggaaatt ccctagcatt ccatttagaa aagtaaacag atttagccag
     9781 cttggcaact tccattcctt cttctggtgt cctaaaacaa acacgaacaa gatgaacagc
     9841 agcaagaaac cataaatagc aagatcaaaa agcctttcat cgaataacaa tcgggcattt
     9901 ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa caagctctcg cgtagctacc
     9961 agatttcttt tgatgtcttt gaaatccagt tcgtaacgcg gataatgact aaccgtatgg
    10021 taggccatga cgtaaaaaag tgtataaata gtaaagcttg ctgcaatctg agtcggtgca
    10081 ggcaactcaa tcgcttgata caggagaaat agccagatag ctgcaaataa gtactttttc
    10141 ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat tgaccgtggt attagcaggt
    10201 acaagccatg ctgcactcag ccctagcaaa acacttgaaa aataaaataa aggaaaataa
    10261 aatacgccta atattccaag caatgaacca gcaccaccaa tcagcagagc gatcatcagc
    10321 aacgtgtaac tatctagacc aaatcgtaga ctgttgacta aaaatagtcc agtcatccga
    10381 aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat tatgcgagtc tgttagtccc
    10441 aaaaataaaa gataaggaat aaaaataacc gtgttactta agataaaggg cgttgtttgt
    10501 gtaaaattac gcccaaatcg tttgagtttt tcgaacatga caatggctcc ttaaacagtc
    10561 ttataactaa tctgctcttc gattttagca ttaaagggga aataaaccaa ttttacagaa
    10621 tatattcttg taaaaaaaaa tacagaaaga agcataagct tctttctgca tttttacggt
    10681 acttgattac tcaagcagac aagcccttct ccacaaaagt cttgcccgac acccctgcat
    10741 ccggcaggtg ataaagatag aaatcaatta tcctgtgcct ccaaaatcaa ttaggagctc
    10801 tgtaaaccag agtccttctc gacccgtcgc ataaatttac tttaacacag ttcctatgtt
    10861 tatgcgagag cttttttcat tttataatcg accatttgaa atccttgacc gatatctgaa
    10921 ataagttctt ctgtaatctc aaatccttca tgcttataaa cctcgattgc ttgatgattc
    10981 ccctgattca ctcgcaaaaa gatctctctt tcctttgttt gcgcagcaag ttcttcatac
    11041 cagcgaaaaa ctgaagaagt caatccttga ccacgtactt tatttagcaa atataattta
    11101 cttaaataaa tttttccatt tatctcttca taagcagtgt aaccgacagt cttttcttcg
    11161 ctttttaata aaaagtagga cacaccttct gtcagttctt tttgaatctg tttttttgac
    11221 tgataagttt tcagcatgta atccacttgt ttttgaccaa tgatcggcac aaaagcttcc
    11281 ggccatactt cttgtatgac tgccatcagc tcatccagtt cattttctgt tacttttatt
    11341 ttttccatac acccacgtcc ttttttacat tataaatgac cttgcgcttg atggaataaa
    11401 aagagcatga tcatgctata cacacccacc gaccatggtt tcaagaaaat cacaatctgc
    11461 ataaattccc taaaagataa tttcgtcaaa ccagctacta gacagatcaa gtcgtctggc
    11521 gcaaagggaa gcagcaaggt aacaactagt acttttttaa tatctttcgt ttttcttgtc
    11581 agcaattcat caaatttctg atactttttt ggcgataaaa taagctggac aaaggctacg
    11641 ccatagtaac ggacaagcaa aaatccgatt gcttccccaa tcactaggcc aatacaacta
    11701 tataaaagcc caaatccatt gccaaacatc agcatgcccg ctactgagga gatccctccc
    11761 ggcaaaatag gaatgatcac ttggatcact tgcaacagaa caaatatcat cactgcatac
    11821 ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg atcgaaaaat cccatgatga
    11881 taagcataaa gaatcaacac aaagaaaaaa aacagaccaa taatgggcat gatctgaaga
    11941 catttttgta aaactgatcc ttttttagac atctatattc cccacatctt ttttcattta
    12001 ttaaaactgc ctccttatga tgtgcacccc aaaagttaga ctagaaatct aattaaaagg
    12061 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
    12121 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
    12181 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
    12241 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
    12301 tatccaagaa actgctaaaa aatttaatat cggtt
//
LOCUS       DACEOM010000087        12074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEOM010000087 DACEOM010000000
VERSION     DACEOM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.090"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..1440)
                     /locus_tag="IW781_002511"
     CDS             complement(133..1440)
                     /locus_tag="IW781_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1183492.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1443..2024)
                     /locus_tag="IW781_002512"
     CDS             complement(1443..2024)
                     /locus_tag="IW781_002512"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1183493.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2140..2237)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2596..3888)
                     /locus_tag="IW781_002513"
     CDS             complement(2596..3888)
                     /locus_tag="IW781_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1183494.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3885..4508)
                     /locus_tag="IW781_002514"
     CDS             complement(3885..4508)
                     /locus_tag="IW781_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183495.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4511..5347)
                     /locus_tag="IW781_002515"
     CDS             complement(4511..5347)
                     /locus_tag="IW781_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1183496.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5364..6251)
                     /locus_tag="IW781_002516"
     CDS             complement(5364..6251)
                     /locus_tag="IW781_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1183497.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6267..7550)
                     /locus_tag="IW781_002517"
     CDS             complement(6267..7550)
                     /locus_tag="IW781_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1183498.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8233..8916)
                     /locus_tag="IW781_002518"
     CDS             complement(8233..8916)
                     /locus_tag="IW781_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1183499.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8934..9551)
                     /locus_tag="IW781_002519"
     CDS             complement(8934..9551)
                     /locus_tag="IW781_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1183500.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9561..10385)
                     /locus_tag="IW781_002520"
     CDS             complement(9561..10385)
                     /locus_tag="IW781_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1183501.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10378..10938)
                     /locus_tag="IW781_002521"
     CDS             complement(10378..10938)
                     /locus_tag="IW781_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183502.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11112..12068
                     /locus_tag="IW781_002522"
     CDS             11112..12068
                     /locus_tag="IW781_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1183503.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         3630 a         2653 c         2047 g         3744 t
ORIGIN      
        1 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
       61 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
      121 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
      181 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
      241 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
      301 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
      361 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
      421 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
      481 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
      541 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
      601 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
      661 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
      721 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
      781 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
      841 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
      901 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
      961 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     1021 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     1081 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     1141 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     1201 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     1261 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     1321 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     1381 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     1441 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     1501 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     1561 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     1621 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     1681 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     1741 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     1801 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     1861 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     1921 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     1981 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     2041 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     2101 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     2161 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     2221 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     2281 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     2341 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     2401 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     2461 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     2521 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     2581 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     2641 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     2701 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     2761 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     2821 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     2881 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     2941 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     3001 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     3061 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     3121 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     3181 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     3241 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     3301 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     3361 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     3421 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     3481 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     3541 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     3601 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     3661 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     3721 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     3781 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     3841 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     3901 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     3961 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     4021 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     4081 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     4141 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     4201 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     4261 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     4321 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     4381 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     4441 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     4501 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     4561 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     4621 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     4681 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     4741 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     4801 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     4861 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     4921 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     4981 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     5041 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     5101 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     5161 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     5221 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     5281 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     5341 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     5401 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     5461 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     5521 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     5581 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     5641 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     5701 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     5761 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     5821 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     5881 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     5941 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     6001 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     6061 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     6121 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     6181 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     6241 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     6301 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     6361 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     6421 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     6481 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     6541 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     6601 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     6661 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     6721 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
     6781 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
     6841 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
     6901 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
     6961 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
     7021 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
     7081 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
     7141 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
     7201 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
     7261 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
     7321 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
     7381 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
     7441 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
     7501 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
     7561 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
     7621 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
     7681 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
     7741 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
     7801 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
     7861 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
     7921 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
     7981 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
     8041 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
     8101 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
     8161 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
     8221 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
     8281 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
     8341 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
     8401 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
     8461 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
     8521 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
     8581 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
     8641 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
     8701 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
     8761 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
     8821 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
     8881 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
     8941 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
     9001 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
     9061 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
     9121 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
     9181 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
     9241 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
     9301 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
     9361 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
     9421 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
     9481 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
     9541 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
     9601 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
     9661 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
     9721 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
     9781 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
     9841 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
     9901 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
     9961 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    10021 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    10081 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    10141 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    10201 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    10261 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    10321 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    10381 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    10441 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    10501 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    10561 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    10621 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    10681 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    10741 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    10801 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    10861 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    10921 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    10981 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    11041 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    11101 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    11161 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    11221 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    11281 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    11341 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    11401 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    11461 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    11521 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    11581 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    11641 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    11701 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    11761 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    11821 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    11881 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    11941 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    12001 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    12061 atatgtaatt ttaa
//
LOCUS       DACEOM010000088        11411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEOM010000088 DACEOM010000000
VERSION     DACEOM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.091"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..2522)
                     /locus_tag="IW781_002523"
     CDS             complement(159..2522)
                     /locus_tag="IW781_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1183504.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            complement(2571..2948)
                     /locus_tag="IW781_002524"
     CDS             complement(2571..2948)
                     /locus_tag="IW781_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ1183505.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLIC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            complement(3083..3382)
                     /locus_tag="IW781_002525"
     CDS             complement(3083..3382)
                     /locus_tag="IW781_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1183506.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            complement(3616..5217)
                     /locus_tag="IW781_002526"
     CDS             complement(3616..5217)
                     /locus_tag="IW781_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1183507.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            complement(5614..5868)
                     /locus_tag="IW781_002527"
     CDS             complement(5614..5868)
                     /locus_tag="IW781_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183508.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            6434..6730
                     /locus_tag="IW781_002528"
     CDS             6434..6730
                     /locus_tag="IW781_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183509.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            6737..8689
                     /locus_tag="IW781_002529"
     CDS             6737..8689
                     /locus_tag="IW781_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1183510.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(8761..9258)
                     /gene="dfrG"
                     /locus_tag="IW781_002530"
     CDS             complement(8761..9258)
                     /gene="dfrG"
                     /locus_tag="IW781_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1183511.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(9652..10536)
                     /locus_tag="IW781_002531"
     CDS             complement(9652..10536)
                     /locus_tag="IW781_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ1183512.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            complement(10523..11086)
                     /locus_tag="IW781_002532"
     CDS             complement(10523..11086)
                     /locus_tag="IW781_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183513.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
BASE COUNT         3706 a         2021 c         1525 g         4159 t
ORIGIN      
        1 ttctacttcc aatttaacaa aatagcaatc taatttgttt gtattttttc aactgtcctc
       61 taactagttt tgtatttatc cttaattcag tatacctata ttttgggcac ttttgaataa
      121 aaataagcgg aaaaacaaat gatttccgcc actaagaatt aaaattcttt ctttataact
      181 ttgtttttat tatcaaagac aagataagca ctatattttt tattgtcttt acttataaaa
      241 cccgttactt tctctttagt tttcccattt tctaacaatt ctttgactgt ttttgaactt
      301 aaagttttcc cactattatt tttatagaaa ataaaacttt cacctgttgc taattctttt
      361 atttcaaata cttttgcttt ttcaactatt tcataaccat ttactgtatc gtgtttgctc
      421 tttttatcgc cttttggact gttcttttca aactccatta ccactttcat cacatttgga
      481 tcgaaaagta atcttgcatt aaaagctttt ccttcttttg acttaaagcc ggaaaggact
      541 ttatctgtac gattatttag taatagttct tgaagaactt ttttattgat tgcttttcct
      601 gatatatttt taaatatcac gaactgttca ccactagtaa tatttaaaat agaataaact
      661 tttttctttt cttccacttg ataactacct atatcaaatt tattttgatt tacagtcttt
      721 tctaaataat ccgaagtaaa ttctactttt ttcaaatctg aaacagtctt attgatatag
      781 ttatagatat ttcttaaaaa agcttcctgt gtatagctac cttgcgcaat tttcccaagt
      841 gattcttccc atatcgctgt attttctggt tttccaatca ataggtcttt attctttgtc
      901 aaatcccata ataaatatcc ctttggagta ggataaacat tcgtgttttc tatcttaata
      961 tattccctat ttagaagagt gtctacaata ttagaacgtg tagcgggcgt tcctagccct
     1021 aatttcgcca taataccggt tttacctaat aatttacctt cagtcaacct tttaggttcc
     1081 ttagttaatt tatcaataaa tctaatatct atatttattg tctcttgttc atgaaattta
     1141 ggtaatgttt cctctttatc atcagattca tctaaattag tatctacttg cttccaacct
     1201 aaagatatag gtacattacc acttgtggta aattctaagc cgtttacatc taatgtaact
     1261 tttgtatttt cgtacttgta aggttctgca aacattagac acgcacgcct agtaacgatc
     1321 tcataaatat ttttttcatc taatgtcaga tttgctatat caggaatatc cattgttggt
     1381 attagtgcat aatgctctaa tactttgtca ttatctacaa accgcttttt aggctctaaa
     1441 caaggtgtat caatactgat ccctaaaaca ctcttataca cgtctaaatt cgcttttaaa
     1501 tactcaaatt catttgtagt tatcaattca ctatcggttc ttggataaga taaatatcca
     1561 cgatcatata gactttggat aattttatct gtttttttag cagaatattt ccatttttta
     1621 ttagcgtatt cttgaatacc acccaagcta aacaattttt gtggttctgt ttgcttttta
     1681 ctaacctcaa ttttttttat gattccagta ctggcggaat caaggccgaa ttttttagtg
     1741 tcttcttcaa atttttctaa tttaaagtat tcaagactat tcttaaatat tgcttttggt
     1801 tgcttatcgg ttgtatgaca ttcaatcgtt ttatattttt ccggtctaaa atttctaatt
     1861 gccaaatcgt tttcaaccac aagtgtgttt accggtgtct gaactcgacc gagtgatacc
     1921 acacccgata tacctttttc ttgcaacttt aatgaataca actctgtata attcatccct
     1981 actagatagt cagcaatttg tctagcatta gcttctttga agtaagagat cgtttcttca
     2041 gaatttcgca gatttttaaa tcctttgata atttcttttt gaaccaaaga attaatccat
     2101 aatcttttta tcggtttatt aaaaatcgac tgcttgcata atttgaaaat attataagca
     2161 atattttctc cttctctatc cggatcagta gcaattatta tctcatctgc attttctaaa
     2221 catgacttca ctattgaaaa ttgttggttt tttcctttag agacttcata ctttaattca
     2281 ttgaatttag gtagaaaagg caaattatct aaatgccatt tatctttatt cggtagccac
     2341 tcatactttt caggtaatga taatcctacc aaatgaccta ttccccaagt aacaatagta
     2401 tctccaaata tttcactttt aaattcatac ttcccactct ttttcgataa tttttttccg
     2461 gccaatgctg aagcaaattt tgctccttga tctggttttt ctgctaatat aactactgcc
     2521 atatacttcc tccactttct tcaattgatt tttaaccaaa agttcttact ctatgactga
     2581 ctacttgtag tcgaaagtag tatatctcta attcttgtaa atttagcatt acttgtccgt
     2641 tcacaaatta aagttatttt tgatcgatca ggtaaaaatc gtacactttc tttagccaca
     2701 tctacttccc ttttaggtac aggttcacaa caatattttg tattactcca aaacgaaaat
     2761 tttaactttc ttccctcaat cgtatcaaca agtaaaatta tatttccttt cttcccccaa
     2821 tacctacaga cagtttcacc tacaatttta aataaaccta tttcatcagg aaataaatac
     2881 tcttttctat caatatctac tatgcctttt ttaaaatctg attcattata aatcccatca
     2941 atattcatta atagccccct tttttattta cctgatatat attaattcta acaaaaaaac
     3001 agccaatatc aaactatcaa aaaacagagt agctatctgt ctacttgtag tcaaaaaaac
     3061 aaattattct tttcttcttt ctctaatctt caatatattt tttattccgt tttggggctg
     3121 aaattttcaa atactcttta tactcttttt catactcaca caagcaatca taaacctcat
     3181 tcatattccc tcctgaagct agagacaaag catacaaaaa agaaataggc aaatcagcta
     3241 cctcacgctc tctagtaatc caagacgata gagttccttg tttaaatcca tataactcac
     3301 aaaacacctc aacagataaa cctagcttac cgataataaa tacattgatc gggtgtgagt
     3361 aaacatattt cttaattacc aaagaaaatc ttcctccttc aaacataact gcatacgcag
     3421 ttataagctg tattccgtac acgtggatta agggaaaaga cacaagtaaa aatagttcta
     3481 tacactccat acataaaaga gagtactaac tactaatcat tagtatacgc taaaacagga
     3541 ataggagtgg gcagaaacac tagatatgtt aattggcaat ttagctattt aaaaatcaaa
     3601 aaaataaata gaactctaac ctgtattagc tcctctaatt ggcgcatgtt tgctcttttc
     3661 tctttctttc agtatgttcg catggaacgg tactgttttg tcttcataac gtcttaaaat
     3721 cgtttctacg tggctatttg ctgttcctaa taattctaag acaaatactt gtttatcttt
     3781 tttattctga ataactgtat ttaatatttt agcttccaca aatttgcttg ttgctatttt
     3841 agtcgatcca taccgaccac gagacgttcg taatatcact gtattcgccg ttctaagctc
     3901 ttttaaaatc acctttaacg tagatacagg aatttgtgtt tcttcagata tagttcccat
     3961 agagacactt aaaaacaact catttttttt caatgatctt tgttttcttt ctaagtaagc
     4021 taataaatca gctttacttt cttcaaaatg actatatttt cgttcttctc taggtttagc
     4081 aaatttcacc caattagacg gatcgacacg attcccttta cgtttaacag cttgtctatt
     4141 tacatttatt gagtattttt caaactcggt aggtgtagca taattttcga taatttcatt
     4201 gacatatgaa cttttagcac ctttgtaatc gccacgataa gcacttctta ttgttctctc
     4261 aacttcagaa taggacaaat cattattgaa ttgaagttga tccaattgat ccatacactc
     4321 gccaatagat aagccacttg atttacatgc cagggctaag gtaaaaatag ttttatttct
     4381 atctccttca gcaatatccg tatgaattaa aaagtcgtac catttagtat caatttgtcg
     4441 tttagaccct ttcttgccag tagaattagg agctaataat gacaagttag acctctttaa
     4501 agcatattct ttttcttcaa agtcttttga ccatttcaaa tacccttcaa acgtatctac
     4561 gaaattaggt tcataatgta caatattttt tttagttgga aatctaaaaa taccaaaatg
     4621 attacagccc atatcgactt gtggtaaacg attttggata gattttctga tattttctga
     4681 tattctttta gcaactctca aagatttaaa atcatttgaa ttagaaatat agcttgcctt
     4741 atcaaatgaa tcagtttctt ggttatagtt ttgaatcaga aaatagcaat gataaccttt
     4801 tggcgtatct acaatcagtg taggcaataa cccttcttcc aatagataaa gaactagtaa
     4861 attcaaatcc gttttctttt ctcctactgg atagtcaata tctgctacaa aagtattaat
     4921 ctgaattaag ttagcttctt tgtgaccaaa aattgttttg ttttcaccgc caccaagcca
     4981 tgaatacacg ttaggagtcc agtgtgttaa tacatctgaa tcttccaaga cacgatcaat
     5041 agaacgatat ataagacctt tagtctttat catgtttgtt ttagaagtaa atccggcaat
     5101 agcaccaatc ttattgctat tctttttaaa agcaactaga tttttgctat taaactgttt
     5161 aattccattt ttaagaattt tagctaaaac gtccgcatac tcgcaactat tcaacattgc
     5221 actttctcct tgctatcaat tttgaatagc aaaaaagaca tagggtaact ttagattccg
     5281 taataaggag tcctaaaatt acactatgtc tttagagtaa gcacttaaat gctaaacttt
     5341 aaacgtgcaa aaattaaaat gagttggtag ctcatcttgg tggacttcgt ggattgccaa
     5401 atcgcttttc ttactacaga agttccatta aataagattg gtagtcttat ttaattatct
     5461 ttttcgctat ttttttcgtt acattcatac atagtagaga gtctgtaact aacttttcta
     5521 ctagaatttt ttagtgttcc tttggcttca gaactaatac caacattata agctaataga
     5581 attactttta acaagagttt ttttaaaaga tttttacccc atcactttta cttgatttat
     5641 aacataagta tttttatcaa taatttcatg aatgcaatat ctgattttta aagtatttat
     5701 ttttttcagt gttgtttcgt ctttgggaaa aggcagtttc caaattagaa tacatcctga
     5761 ttcagtatta aaaattattt cagtttctga aatatagtac ttacttttaa caagtttaac
     5821 attacctatt tttacgatct catctatttg atctcctacg ttatacattt ttgtttctcc
     5881 tttcttgata tatatattat atcaccttga ctacttgtag tcaacttgga ataaaagaaa
     5941 tagtctactt gtagtcatgt tttcaattgg aaaaaaattt atttaaagat tcttttaagg
     6001 aaacgcttaa acaatatttc ttagcttttt tacttgcaaa ttcaggaaag tgactgtata
     6061 atcatttata taaattatga aataaagact tcgtggattg ccaaagattt ttcttactac
     6121 gaacagtctt acgagtcaac gattgttggt agctttcgtt ggctctttat ttttgccaca
     6181 aaagactact tgtagtcttt tgaaatttca ttttcaggct acttgtagtc aaaaaaaaag
     6241 gaaagacatt ttcatttaag tagttcagtt ttggagtaca aaaagtgaaa atgtaaacct
     6301 aaatgagtat caaattgcaa ctgaatttga tactcgtttt ttattgttaa atcaacgttt
     6361 gttgttaatt tacaccacaa aatgtaagtt aaaatgtata taaaaatgcg aacgtgaaat
     6421 aattattgta ggaatgcaaa ttggtgtgtt aaaatggtta caatttacta agcaaaaagg
     6481 ggtgcatttt cctatgcaat ttcttgagcc aaaaaataag aacgctaagt ctgtggattg
     6541 ggaaatatca gagcaagtaa gggtgatagt taagcaatac gctgaatatg cagagcgtac
     6601 tgaaagcgaa gcagtggatg aattcttatt gaacatttta gacgataaga agtttattga
     6661 atggattgcc aacaagcgaa gtaataagcg gattgttgaa aaaatgggta tcaaagatag
     6721 agtgggttga tagacaatga ccaagcttaa aagattagcc acgaaggaag atgaaattgt
     6781 agatgttaaa attccgattt caaacgaaga attaaaagat agggctaaac aatatgattt
     6841 attaactcca aaacgatttg caacaaggta taacaaaatg ttgttcttgc caacgtcttt
     6901 taaatggaac ggttctgaat atcccattca atataattac tgcatcaatc cgttttgttg
     6961 taattttggg aaagaacagc acaaattcaa agatgtaaaa gggaaaccaa gtcgatataa
     7021 aatgactggt agttccaaag ataaaggtca taaaggtatg tactgcaatg acaatcctat
     7081 tggcagaggt gtatcgcaaa actgtacagt aaccccttta tctaattggt ctgttgttga
     7141 agaaattaaa cgccttatag aaatcaatag catccaagat gttgagccag attatcaatt
     7201 tcataaagaa ggctgctcgg aagaagaatc aaccccattc aatgagccga agcaatttta
     7261 caaaaggggg aaaagtaggg gcaaatctca acgctatcaa tgtaaggcgt gtaagaagtt
     7321 cactaatgtt ctaccaaaaa gagaagaaac cactacttat caccaacaaa agaacaccat
     7381 tcttccaatg ttcgctaaga tggttgttgg aagggtttca gtcagtcgta cttgtgatat
     7441 tttaggaatt ggagttggaa cctactatca taagctggag tggctgtata ggcgttgttt
     7501 ggagttttta gaacgatatg agacacaacc ccttcaaaca aagaaattta acgagatgtg
     7561 gcttaatacg gacaagatgc actactacct aaataacgtc cgtaagaaag gacaaggttc
     7621 aaagaaatat acggggtttg aagaccttaa tatgcaaacg tatatagttg tatctgctga
     7681 ggtcttatca agatatgtgt tccgttctga tgttgcctat gattggaata tttcaatgga
     7741 tgaattaaat gaagatacaa gaaaattcaa agaagaccat ctcaatactt ttagtcgtaa
     7801 aaatgatagg ttagattggt cttattatcc acaagaacca tccgctaacg atagcgaaaa
     7861 taggaatgct tatcttcacg aattagggaa aattactaat aggagtagat ttgtcgatgg
     7921 tttaaatgtt gatgctcctt atactacaac tgctcactat tggttaatta aacaaatggt
     7981 taatgccgac gaatggcgaa tgattagtga tgatgacttc tctattagaa acgcctttta
     8041 tagagtattc acgaaagaat taaggttgag cgatgcacat cattttattt gccaagttaa
     8101 caagacgaaa agcagaaaac aatgcttaaa agaattcgga caagcaaaag cagaactgct
     8161 cgattgggga gatatcagag gattcaaaac aaagtttcta cggactttgg catctcatta
     8221 tctaactgaa ttactaacaa gtcatcaatt ccacgaagag gctattagta aagatggaga
     8281 aagatatcgc aaatatgccg ataaccctat caaacatcca ttagccacta aggataaagg
     8341 attttattca gttgattgta gaacggattt atctgctctg gaaccaaacg aaatcgcaaa
     8401 gatgctatta aatgttaatg accattcgac caatagcttt attcaacaga taagacgtta
     8461 tatatcatct cttgaaagac cattaacaac agctcgtggt gataagaaga gctatattta
     8521 cgctaacttc aatccgaagt atgctcaatt tgcgattaca attctaagaa cttactacaa
     8581 cttttgtcgc ccttttaaat cagcggataa aaaggttttg acacctgctc aaagattagg
     8641 gattacagat aaacaatttg attggaaaga tataatttat ttcaaatgac aaaaagagct
     8701 tggagtaatc ccaagctctt gtgctttatc tttactgttc ttcttcaatt aacgcacccg
     8761 ttaactcaat aagttttttc tttcatatac atgaaaatag tagttatacg gatttttatc
     8821 attctttatc cctttttggg caaatacctc attccattcc tcataattca cttctggaaa
     8881 aaaagtatct ccttcgaatt catgatgtat ttttgtgatg tacattttct caacataagg
     8941 gaaaaacaaa ttataaatct gttctcctcc gaaaataaaa atttcttctt cgtttttaca
     9001 taactcaaaa acatcttcta ttgaatgaac aatttcacaa ccattaaagg taaacccctt
     9061 atctctcgtc agaataatat ttcttctgtc aggtaaggct cttccgattg attcaaggtt
     9121 cttcctacct aatattatcg gatgtccctt tgtagtattt ttaacatatt cccagtcctt
     9181 gggaatcctc caaggaatgt cattctcttt gccaatcact ctattcttat ccatcgcagc
     9241 aatcaaagaa actttcattt ataacacagc ctttttaact aatttaccat ctaatttctt
     9301 atttctatca aaaaaccttt tctcattatc ctgcccgtta gttgaataag caattttttc
     9361 tataagttat attatcataa taccacaaaa tcacaaattt tcattcaatt atcattttca
     9421 attgcatttt ataaatcatt ttgtatgtaa tttggtatgt aagttaaaat gaaaaaagta
     9481 gtccattttg aagtacattt tgtcattttg tactccaaaa ctgaactact taattttcat
     9541 tgtctttcct tttttttacg atctttcgtt agtcttctgt acggcccaat cgttcttgta
     9601 ctttcttttg aaggcgttct tctttcagat aatgtttttc tgtacactat gttagtgaac
     9661 ttcagcaaat tcattttctg ccccctgttc actaagctgt tttggattcc aaactagttc
     9721 ataatctcca aagaaatctt tcaaaaccat aggttcatta ttaaatattg ctcctgtgtc
     9781 gtacaggctc tcctgaagag ttttgttata actaccagtt aaaaatactt ctttacttct
     9841 atagatatat ttatcccact tactatccat tttaaataaa aacagctcaa cattttttaa
     9901 atttccataa ggtgttttta aacaatagct ttccttttga atatcagaaa agacttgctt
     9961 tacaatatgt ttaaacagca ttttttcagc tatattacta actaaatata ggagtaaaaa
    10021 tgcaccaagt ttagttgtcg tattataata attttgaaaa tgaatttcat gctcatacag
    10081 gctaagtaga aataaaactg tgccctgtct acaaatgatt tttattcctt tttgaaaagg
    10141 cttcaaataa ttaatataat tgtcatttgt tgacatcatt atttttccta cttgtaagat
    10201 aaataaagca aaaataaaca ggctgaaagc aagacttcct aaaatattta aggcttcttt
    10261 cacctctaat gattctttta atcgatcagt tccggcgaac agtttataaa agctataagc
    10321 attgctccat cctactctag caatgaatag ccagaccaga taacctaaac aactaaaact
    10381 aattttcagc cctctcacta tatctaattg aaaaatgagt ctaaattccg gctctctata
    10441 catactttta aatttaacaa tttctttttt caatccgagc gataatgact ctttgcctat
    10501 ttttttactc attttactcc ccttaaaccg cttcatcagc gatttctttc tcataaagtg
    10561 aataaaaaac agacaattta attggctcta aatattctaa agttgcttca gggattcttt
    10621 tactgtcttt aaccgcaata taagctgaat cattaaaata aaaaaatttt tctacattac
    10681 tttttgcact aaaaccatca taaaaaattc caaaaagttc tttactacgt ttttttgaaa
    10741 atggattcac caattcaaaa acacgctttg aaattttttc tagcaattct ttatttgttc
    10801 tatagaattt actattttta ttgattcctt ttaaatcttc tcgaccacta atactatttt
    10861 tgaatgtagt tactttattt ctactataaa attctccata tattgatact tctaaagtac
    10921 catcttgcca taccaccaaa tgatctatat ctataggatc acttaattta ctgattaaat
    10981 cagcaacacc attttctaaa atgtaatcta aattattatt taatactctc gacttaacgt
    11041 actctgatac gttagaattt tttagtgctt tataaatagc tgtcattctt tattctcctt
    11101 cgtagccttt tgatctacga atttaactac cctttttcta tatacaaccc tcctcattca
    11161 aaattagata gtcaagacgc ttaacaagac tacatttcta ttttgtatga tttaccgcta
    11221 taattcaacc tcaattaaaa tttatttcgt gcctaacgat ttaaatattc agttactttc
    11281 tttattaaaa acagcaaact ataaatgata ataaaatcaa acactattaa aatcatactt
    11341 gatattaaag ctttatttgt taatgttgta atcccaatag taattactat taagtttgtg
    11401 attataattc t
//
LOCUS       DACEOM010000089        10931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEOM010000089 DACEOM010000000
VERSION     DACEOM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.092"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..407
                     /locus_tag="IW781_002533"
                     /pseudo
     CDS             1..407
                     /locus_tag="IW781_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(309..1025)
                     /locus_tag="IW781_002534"
     CDS             complement(309..1025)
                     /locus_tag="IW781_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1183514.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1075..1284)
                     /locus_tag="IW781_002535"
     CDS             complement(1075..1284)
                     /locus_tag="IW781_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183515.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1561..2834
                     /locus_tag="IW781_002536"
                     /pseudo
     CDS             1561..2834
                     /locus_tag="IW781_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2941..3972)
                     /locus_tag="IW781_002537"
     CDS             complement(2941..3972)
                     /locus_tag="IW781_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183516.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3979..4809)
                     /locus_tag="IW781_002538"
     CDS             complement(3979..4809)
                     /locus_tag="IW781_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1183517.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4806..5612)
                     /locus_tag="IW781_002539"
     CDS             complement(4806..5612)
                     /locus_tag="IW781_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1183518.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5618..6442)
                     /locus_tag="IW781_002540"
     CDS             complement(5618..6442)
                     /locus_tag="IW781_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1183519.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6432..7532)
                     /locus_tag="IW781_002541"
     CDS             complement(6432..7532)
                     /locus_tag="IW781_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1183520.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7710..8495
                     /locus_tag="IW781_002542"
     CDS             7710..8495
                     /locus_tag="IW781_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1183521.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8528..8911
                     /locus_tag="IW781_002543"
     CDS             8528..8911
                     /locus_tag="IW781_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1183522.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8987..9118
                     /locus_tag="IW781_002544"
     CDS             8987..9118
                     /locus_tag="IW781_002544"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1183523.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9087..9323
                     /locus_tag="IW781_002545"
     CDS             9087..9323
                     /locus_tag="IW781_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1183524.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9401..10372)
                     /locus_tag="IW781_002546"
     CDS             complement(9401..10372)
                     /locus_tag="IW781_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1183525.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3249 a         2542 c         2088 g         3052 t
ORIGIN      
        1 agattggatg actcaccttc ccattgtgaa cactgtggat cgattcgtat tatccgcaac
       61 ggttcctata cgacacgtac tcagatgttg aaagtcaaag aaaagttgac tattttagaa
      121 ttaaaacgta cccgttttct atgctatgat tgtgggcaga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct catagaactg
      241 tacgaaaatc aatcacgtaa tctgaaaatc agaagaagta ccgtcggctc aagcgatatt
      301 ggaaactctt actcaaagat tccacgacat tggagccgct caaacggcat tatcatcgac
      361 tgtttaaacg accaattagt caaacagaaa tcgtcgatga accgttgtgc acaatggtgt
      421 cctgaacgta cagcgacacc ttccatatcc agagcagtcg ccacatcacg gggatggatg
      481 ccatccaaat taaaagcaac cacgccagta tgatgttcag gatcttgcgg tccgtaaatg
      541 gtgataccgc cgatcgcttg taatttaggt aaaacgtagg caaccagttc ttgctcataa
      601 cgatgaatat tctccatacc aatctcattc aagtaatcga ttgctcctgc aatgttcgga
      661 gtaccagctt caaatttcca tggcagctct ttccatgaac tatcgtaaag attgacaaaa
      721 tctatcattt caccgccgaa ttcgactggc tccatttgtt ctaacaattg acgtttgcca
      781 tataatacac cgattcctgt cggcccgcac attttatgcc cgctaaaggc gaaaaaatct
      841 gcatccaacg cttgtacatc aactgtcata tgcggtgctg cttgggcacc gactggacta
      901 tcctcaacta caacagcctg ttcaaccctc agccgtttca agcacaacaa ataaccttct
      961 ggatctggtt tgccgacctg aagatcatcg atcgtcacaa aggaatcaat tttttcctta
     1021 atcatcggat gtgcttcaaa aaaatagtct agctcccgca gttcagaatt gagactactc
     1081 cgattttcca gtgctgctga tccgctgctc aaaatgttca gcatccactg gtctcccaac
     1141 cgcttgactt atcccttcat aaaaggacag ttctgctgcc gtcccaacac cttgttgata
     1201 gtctgcaaaa ggaattttat agccgaactg ctccagtaaa taggcttcag taaatagtga
     1261 tgaatactct cgtaaaacat ccattcactg tcaatcatcg tcccatcaaa atcaaaaata
     1321 agtgctttgg tcatcttctt tcccccttac ccaaaacaag caaaagctgt gtcctgctct
     1381 ctctacagta tcagcataaa aactgccgtc tgaaaagtca aaggtgaaag aaatgtggcg
     1441 ctataatttc taggactgat agtaaatcat tctatcagtc cttttgagca taaaaaaaca
     1501 aaacatacag aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt
     1561 atgcctgatc ttcatcttat caaactatta ctaggaatca aagatggaaa cattcaaatc
     1621 actcatgtcg agaagaaaaa aatacattca atcaacagta tcgttgtttc tgccacgctc
     1681 attaaaacga ttcagccctg tcctcagtgt aaacaggtga atcaacatgg gctgatcgtt
     1741 aagaacggaa agaaaaaatc gctggtcctt ctcaataagt gtgcgaatca acggacttat
     1801 cttgctttgg caaagcaacg gtacctctgt cgtggctgtc acacctattt tacggcgaat
     1861 acctacattg ttgaaaaaaa ctgttttatc gccaaacaag tacattataa aatattggag
     1921 gaattgactg aaaaacaagc aatgacgacg atttctaaac attgcggcgt ttcttggtca
     1981 accgtttcgc gaacactcgc ttctctgctc cccatgacaa acgtgaaaat aaattggttg
     2041 cctcgctgtt cattggtaga tgaatttcgt tcattgaaaa atcaagtcgg tccctattct
     2101 ttttcttgta tggatggcga tactgggaaa ttactggata ttcttccttc acgtaagaag
     2161 aaagatctca tttcttattt tatgcaattt gaacgacgtg cacgtttaaa cgtaaaaata
     2221 ctcgtaacag atatgaacgc ctcttatgct ttgctgataa aagagtgctt tcctcatgcc
     2281 aaactcgttg tggatcgctt tcacatcgtc aagcacctca ttcgcagttt taaggatatt
     2341 tgactatgag tcatgaaaag cttcgaccga aacgatccaa tacaagccaa acactatcga
     2401 caagtgaagg ctttatctcg acttctgatc aaacgacagg acacgcttgt ttatgataaa
     2461 tggacaaaat ggcgtaattt tggttgggcc tatttaaccg aaagtgaagt cgtagaacgt
     2521 ctactttcta cttctgatga actacgaata gcttatgctt actatcaaga aattcttcaa
     2581 gctttttatg acaaagaagc ggacatcttc tttcagcttg taaaaacaat gcccaagtcg
     2641 gttccaagag aactgcatca tataaaaaaa gcgtttataa actatgaatc cggcattcgt
     2701 ttagccttag agttgcctta ctcaaatggc aaaatagaaa atctgcatac ccacatcaaa
     2761 gcattgaaac gaatagctta cgggttagga tgagaaccag aattttctta atcaatcatc
     2821 tgatcactta ttaaacataa aaaaacatct aaagcagaaa atcaattcta ccttagatgt
     2881 tcttatatca ctctatcagt cctatttgac aaagagccag aaatgtttgc cgactataca
     2941 ctagctttgt tgtgcaaaat gctgagtatc aatttccaca gggattgcta gaatatcttt
     3001 cagctgaatg tataaggcat cgctaatttt ctcttcccga tcgttcaacc gatccactaa
     3061 aatttctttg ccctccagcg tttgaaaaac ataccgttga atgttgccta aaataaatga
     3121 ttccttccat agaaccggaa tcttaacccc ctgaggaacc ggttcaaatt gaatcagctc
     3181 cggtcttaaa taataaatgc agcgattgcc cttagcggaa aactcgtggg tatactgatt
     3241 cagctcatac cattcgaaac gattatagtt gccgataaat tccgcaacaa atcggctctg
     3301 aggattctga tacaattcct tcgaggtgga aacctgagcc acccgcccag cttccatcac
     3361 aaaaatccga tcactgattc gcatcgcctc ttcttggtca tgagtcacaa aaatcatcgt
     3421 catccctagc gattgctgga tagcgcgcaa atcccgctgc agctgttttc taattcgggc
     3481 atccaaagca cttaatggct catccagcag caggactttc ggttcagtga ccaatgagcg
     3541 agccagtgcc acccgttgtc gttgaccgcc agaaagaaac tggacatttt tatctgcctg
     3601 ctccgtcata tccaccaatg atagcatctc aaaaaccttt tcctgaatca cctcaggact
     3661 cagcttcttt accttcaaac caaaggctac attttcaaaa acagtcatag ttggaaacaa
     3721 tgcatactgt tgaaaaacaa agccgatatt gcgatccttc gtaggcttat cggcaatatt
     3781 ctcattgtcc agcacaatct caccgctctt tggttgttcc aatcccgtga tacagcgcag
     3841 caacgtagac tttccgcacc cgctgggacc tagtaaagta accagctcac ccttttcaat
     3901 ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa aacagattga gatcatgtat
     3961 ttctaaaaac attttccgtc acaactcctt taagggcttc tttttcgact gcttctcttc
     4021 cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa gtcagcataa aggtagttaa
     4081 agcaattccc accataaaca gcaaatacaa aaccatcaca gcgctagcca aatggccgtt
     4141 ttcgttcatc cgccgcagca tatagatgcg taccgtctga aatcgaccgc caatcaacag
     4201 gttcgtcagc atgtactccc caaaaaccga agaaaaaatc atcagagaaa ctagggtcac
     4261 accgatacgg atgtttggaa acagcacctg gataataatc gtagacatcg gggcgcccaa
     4321 ggtttctgct gcatccacta attccttcag attaattaag cgcatctggt tgttaagact
     4381 aagatagaca atcggcaacg ccgtaataaa caatgcacct accaacacta caaacattgg
     4441 gataccagtt tcagcatacg ttcgaagcaa agccgttacc aaaatcactc ctggtaaagc
     4501 atacggcagc aggagcagct tttccagcca acgattcaaa cgagggaaat acaaatgaat
     4561 ccaaataatg gtgggaatca tcaacaacag aatcgtcacc aagaccacac ctgctaataa
     4621 aagtgagcga cccaccgcag caataaattc cctgtccgtc agcagctgtt ggaaccattg
     4681 caaggtaaaa tcactaggta aaagcgattt tgaccattta gtggagctgg catacaaaat
     4741 cgttccaata atcggcagca agtaatacag gacaaagcag aacaaaatcg gttttattaa
     4801 ctttttcacg tctgcaccac cttgcctttc ttttgaataa tcaacattgc cgttaccagc
     4861 aggacaccta aagcgactgc caacgcactg cctaaattag gcttggcgaa cacatctccc
     4921 gacactaaag ccgcaattcg aatcgttaaa agatttacat tactgctggt cagggcatac
     4981 gctgtttcat aggtccccat gccattagca aataagatga caaaggtgct ggcaatcgat
     5041 ggtcgcaaaa aaggcaaagc caccttgaac caagcatacg ctgatgaagc gcccagcaat
     5101 tgtgcggact ccagccattc ctttttcact tccttcatgc tgggatacaa aaacaagacc
     5161 cctaacggaa tttggaaata ggtatacgtg atggtcaatc cccaccaaga atacaaattg
     5221 aagtccgcta gaaatggcaa aatcattttc agcacaccta cattccccaa caaaataata
     5281 aaagagaatg ccagcggaat cccagcaaaa ttagctgcca aattggaaac aaaggtgatt
     5341 ttctcctgca atttagtcga tagattcatt aagcataaac ataaaataaa cgtcccgatt
     5401 aacccaatca cactggagct gacagcaatc agtacgctgt taacaaaggc ctgtgtgtaa
     5461 tatttgttgc tgaaaatatc actgtaattg gccaatgtcc actggttagt taaatcactg
     5521 tgcaagctgc acagaaccat aaaaagcagc ggcagcagta aaaatgcaca gaagataaag
     5581 gaggcaggaa ccagaaccaa gcgttttttc atctctttca tcccctcatc aattcttgaa
     5641 tgtcctgcat tttctctgac ggtgcaattc ctaataacca gcaaaacaat ggagccgcca
     5701 gcagctgtgg tacagtttgt tccttcagct tttggcgttt atttgaaatc aaataaaaag
     5761 gaacttcacg atgtgcagcc aagctgccgc catgcagtcc gtgcgcatcc atcccgtgat
     5821 ccgcagtcac cacgacttga taaccttttt gcagccattc aggaaggtaa atagccaata
     5881 atctgtccgc ctgattaacc gctgctgcat attcatgaga atcggcggta aatttgtgac
     5941 cgacatcatc aatattcatc gaatgtacga ccaaaaagtc tggctgatac tgattcacta
     6001 aataatgggc atccgcaaat aaatgcgaat cagggtaagt gtcctcacta taaaagatgc
     6061 catgctgaat cagtcgctcc tcctgatgct ggatgcgatc aaccaaacga ttataggggg
     6121 cttgattgta caactcgctg taccagtggt aagcggcggc tcctgttttt ccgccagctt
     6181 ccttaactaa ttcaaacagc gaccactctt tggatcgctg agcatggtaa ttactataga
     6241 tgccattttc ataggctgga acgccggtca tcagtacttc gtataacgga cgtgaattgc
     6301 tgggcagctc actttttacc ttcaataagc ttgcttgctg gtgctcaact aaatgattca
     6361 tgaacccaag ctgggcgacg gctgtgtcgt agcgacagcc gtctagtata actacaatca
     6421 gtttgtcaag tctactttgc ataggtcagt acctccgcct gccaaagctc tggaagctct
     6481 acagaggtct tgttccaagc ttcattgtct tcaactggca ccgccgcttc ataagcatcc
     6541 tccggcagta atttttcagc tacttcttta ggcagctcta ccttgcgaat aggtcgcgca
     6601 tagccctttg ccaaattgat ttgcccttca tcagataaaa tatattcccg tgctagcttt
     6661 gccgcattgg gatgaggggc atttttattg attaaggttg tatacccgct aatgatggag
     6721 ccgtctgttg gcacagttac ctcaaaacgc tcggagtcaa tttggtcacg ataatttagt
     6781 ccattgaagt cccaaacaac tgccacatcg atttcacctt tttcaagatt tgctacgctg
     6841 gcatcaacgg tggacaaacg gcctttttct gccatttttt tgtagtaatt cagacctggc
     6901 tgaatatcct tttcgtcacc tccgttggca atcgccgcag ccaatacagc aaattgtgct
     6961 tggttggcaa tcgtcacatc ccccacgcta acttgatagt caccctcttg cagctccttc
     7021 cagctagtcg gtgcttgctt cacattctct ttatcggtga taaaggaaat ggttcccgta
     7081 tagcttaaca accaatcccc atcatcatcc tttgcccaat ccggaatttc ctcccaatag
     7141 ttggtcttat aaggtaaggt taactctttg ctttcagcca acggaccgaa gctgataccg
     7201 acatcaccaa tatcggcggt tccatttttt ccttctgatt caaacttagc caattcttct
     7261 gcactggaca tatccgtatc cacgtgactt aagccgtatt ctttttcaag gtcgttccaa
     7321 gtgcctaccc aatttgccca agtatccggc attcccaagc tggcaatctc tccttcttcc
     7381 ttggcttttt ctataatcgt atctaaagag tcgtctgccg cagccgattg cttcgttgct
     7441 tcacctgagc cgcagcctgt cagcaggata cttactgttg ctgcacaaaa taaaccgata
     7501 gtcatgttac gaattttttc attcattttc attcattctt ctcccctttc gataattcca
     7561 ttctaacaaa gctttgtaat gagagaatca gtttcttgaa aaggtttcat gaacaatgta
     7621 aaggttgtat atggtttgta aatttcgacg aattcccgtt aaccttctaa agcttttgcg
     7681 gtatgatagt agaaaaaagg aggctcccta tgcagtatta tgatcagcat ctccacacgt
     7741 atttttcctt tgattcagag gaaaaatttg aaaactattt agcctatcag ccggagtttt
     7801 tcgtttctac ggatcacttt gatttaaaca atccatctac cggaaaagat gacattccgg
     7861 attatccagc ctatataaac aaactggact ctttaaaaga aacctattcc accaaatttc
     7921 tgcgaggtat cgagattggc gttgttccag gacaagaaga gcggatcgtg gattacctcg
     7981 ctcagcatcc ttatgattta aagctggtaa gtattcatca aaatggccgc tttgattata
     8041 tggacgatgt ggttttaacc aaagatccca ttcaaactac caaggaatat tttgatcaga
     8101 tgttgcaggt cttgacggat tttccagaag gagatatttt gactcacttt gattatggat
     8161 tacgccgctt caccttcaca gcggaagaat tcgcggagca ttttgaggct cagctgctaa
     8221 cgattttcaa aaaggttgtt gaacgagaat tggcgatgga gttaaatgcc aagagcttct
     8281 tgaaatacgg caacgaagac ctttaccgtt atgccgttcc tttgtatcaa agtgtcggcg
     8341 gaaagctctt taccttaggc tcggatgctc atgttgccga agattatcag ctgggcttta
     8401 aaaaaatgag tcagctgttg gcagactgtc aggtagaaaa gttgctagtc attcaaggaa
     8461 aagaacgccg cctgacgccg ctgccgattt tgtgattcct atataataga agaaagtggt
     8521 gaaaaaaatg agcaacctgt gggactacaa tcaagaggca cccatccatt atttaattgc
     8581 gcgacattgg gatgcgttga aaattgaggc ggtttgccga tccttgttag cagccgttcc
     8641 aaaacaacaa ctggaaaatt ttttggtggc tgacagcttg cagcgggaaa aagttcaggc
     8701 ttattttgca gcctttaaag atcagccttt ggaatatttg catgcgcagt ttcatctttt
     8761 ttatcaagta gccgccccag atgactacaa cgatttacga ggacagctgc aattgacctt
     8821 tcaagcggat gaaaccgcct ataccgtttt attgggaatg gctcgccttg gtgatcaggc
     8881 gaaggtcgag tggcggattt tcgatatttg atggcaggat caaggcgttt cagttgcaaa
     8941 ccttcctctc tttcaattag aaaaaccgca gcaggaagaa gtttgtgtgc tggtcaccga
     9001 tgctggtgaa attcgtctcc gcttatttcc tgaccaagct cccctggctg tagccaactg
     9061 gcgctcccta gctgagcaag gcgtttatga caacacacct ttcggctcgt gtcattaagg
     9121 aatttgttat tcaaggtggc gctttggatg gttctggtga tgaagctgtt tccagctatg
     9181 agggcttttt cgctgatgaa gtgcatcgcg gactttatca ttttaatggc gccttggcct
     9241 taggggacca cggcccccac accaacggca accagttttt tattgtgcaa aacacaaagg
     9301 cgcaggctga cctgctaatg taaagcaagc atacttggag cttggaggac taccggagct
     9361 ggatggtcgt tatactgttt ttggacaggt ataccaaggg ttagaaatcg tagcagcgat
     9421 cgcagctcag ccgacaaaca gtgaagatgt accgattgag ccagtgttca ttcgacaaat
     9481 taacttcgaa aaaacatcgt aaaaagacac tcactgactt cagcaaaagc tctattcttg
     9541 agctggctgc agctagtgag tgtctttcat ttatccataa gataaagctt ggattcaata
     9601 aataaataaa tgttttggtt aaagctgtga ccagcaaatg aggaaccgtg cgagccagtt
     9661 gatctgccaa tgcaaacatt tctttaggtg aaggcgctgg cgtgtctgcc aacggatact
     9721 ccagtcccaa tgcttggtaa ccgtgcatac catagcagtg gtacggtagt aattctaacg
     9781 gtacgaaccg atcaagatct gccacaaaac aagcaatcgt cagcagctcg gtcaggttca
     9841 tattgatacc tggaaccaca ggaactcgaa ttcgcacttc gcagttagga tactcaatta
     9901 aaaagcgacg aatattcttc aaaatttgtt ggttatctaa gccggtccat tgtttgtgga
     9961 gcttgctggt aaacattttt acatccacaa acaatgtatc cacataggga gctacctttt
    10021 ggatttcatt ataagcacca caaaagctgg tttccagtac ggtattgatt ccaatgtatt
    10081 tcgatttttg caaaatttct ttagcaaaat ccgcctgaac cagtgcctcg cctccgctaa
    10141 tggtcactcc gccatccgag tgaaagtaaa aaacagcatc cttctcaatc tcatccatta
    10201 cctcttcagc ggtcatggtc tggccaaatc ctttttcaat ctgcttgctt tgtgattcgg
    10261 gaatcgaaca ccaggcacag cgtaatgggc agccctttaa ataaacaacg gtccgtaaac
    10321 cagcaccatc atgtagagaa ccctgttcta ttcttaatac tgttccttcc atatagattt
    10381 ttgaattaca cactagttta aactaaaaca cacaaggaaa aacttaccat ttaatcataa
    10441 aattagctca tataaatata ctaggatgta aaatagactg gtcaaaatct ggtcaaaaaa
    10501 gaggactaat acaggtagtc ctcttttttg ttattttaat attctaaatc attttttatt
    10561 ggtttattcc cttattcgat gtagactcaa atcactatat cactagatat caaacggcgt
    10621 catacggaat caaaaacatc aaatttgacc tgttaaatgg tcattgaata acattcaaaa
    10681 tcaataaaac ggagtcagac acggagtcaa aaacgctgag agtaaaaaat atatacagcc
    10741 ttgtaggtaa agtaccacag ggctgtatat tttttatttc agattacctt aaatgattcc
    10801 atttactttt tatctcattt tttcctacta gttagtatca aatactctcc tattttctca
    10861 ataaactatt tttggataca tggtatctga aacagctaaa accttgtcat tacagcaaaa
    10921 aatcacgtta t
//
LOCUS       DACEOM010000090        10597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEOM010000090 DACEOM010000000
VERSION     DACEOM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.094"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(178..858)
                     /locus_tag="IW781_002547"
                     /pseudo
     CDS             complement(178..858)
                     /locus_tag="IW781_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(932..1582)
                     /locus_tag="IW781_002548"
                     /pseudo
     CDS             complement(932..1582)
                     /locus_tag="IW781_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            1907..2323
                     /locus_tag="IW781_002549"
     CDS             1907..2323
                     /locus_tag="IW781_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1183526.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            2320..2790
                     /locus_tag="IW781_002550"
     CDS             2320..2790
                     /locus_tag="IW781_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1183527.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            2804..3577
                     /locus_tag="IW781_002551"
     CDS             2804..3577
                     /locus_tag="IW781_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1183528.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            3591..4403
                     /locus_tag="IW781_002552"
     CDS             3591..4403
                     /locus_tag="IW781_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1183529.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            4457..5422
                     /locus_tag="IW781_002553"
     CDS             4457..5422
                     /locus_tag="IW781_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1183530.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            5538..8261
                     /locus_tag="IW781_002554"
     CDS             5538..8261
                     /locus_tag="IW781_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1183531.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8274..8906
                     /locus_tag="IW781_002555"
     CDS             8274..8906
                     /locus_tag="IW781_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1183532.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9805..>10597
                     /locus_tag="IW781_002556"
     CDS             9805..>10597
                     /locus_tag="IW781_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1183533.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3519 a         1662 c         2039 g         3377 t
ORIGIN      
        1 gaaaaggaaa acgtagaaaa ttacgtctac gttttcctat ttagttctat atttttatta
       61 aattttatat ttgctctttt cacttttcgg aataaattca ctgtgactct tgataccttc
      121 tttggttctg ttgcgaagtt tgaaaatcaa acgcgccctc tctgaactcc aaatatctta
      181 ggctgttatt cccattaata ccttgatttc agtagacacc gaaaagccga agagcgttcc
      241 atttcttcgg ttctttttat atattcctcg aatggtctcc atgcccttaa tcgtggaaga
      301 ggctgtacgg agactttgat aaaatttatt tcgtcgttta ataggtcgat ggtcttgttc
      361 tattaaattg ttaagatact tcacagttcg gtgctctgtc ttagtatata aacccacact
      421 ctgtaacttt ctaaaggcgg agccaagaga aggtgcttta tcggtcacaa ttgctttcgg
      481 ctcaccaaac tatttatgga gtcgttttaa gaaagcatag gctgcttgcg tatcccgttt
      541 ctttcgtaac cagatatcta aggttaagcc gtccgcttca attgcacgat aaagataatg
      601 ccaacgtccc ttaattttga tataggtttc gtccattttc catgaataga aggattgtct
      661 atttttcttc ttccaaagat aatagaggac tttgctgtac tcttgtaccc aacgataaat
      721 cgtagtatga caaacattta ttccacgatc atataacaat tcctgaactt cacgatagct
      781 tagattgtaa cgcaggtagt aaccaacagc gacaataatg acgtcttttt tgaattgttt
      841 gcctttaaaa tgattcatta ctctgtcctc tctgtctttt ttctcaattt tacactaaaa
      901 tagatttttt ggaaaacttt gcaacagaac cgccaatcat atggaagcca ccgattaggt
      961 attttatggg tttagtcgga aatgtttcaa gagcaatttc catcatatcg ataatgccat
     1021 gatgactaca gccagtaaag acaatcagac cgtcttcttg ctcaattacg accaataatt
     1081 catctgaaaa atcatcataa acaaatttat catccatctt tttgtataaa ttcttggtgg
     1141 tcgagtttgc tttgcctagg gggatgatat ggaagctcgg agcaagttct gttttcgttt
     1201 ttattgtatg taaattctca tgcattttaa ggatttcatt tttaggaata ccaacataca
     1261 gaaacgtggt taaaatcttt aaataatggg gtaaagttac accttcagct gtataaatag
     1321 gggcgtttcc tgattttaaa tatcttaacc cgccggtgtg gtcagaatga ccgtgtgaaa
     1381 taatcactgc ctccagatca ttcaaagttt cccccatttt agtcgcatta tagactaatt
     1441 ttttattagc gccagcatcg aagagatatt tcttccctga gtagttaatt aagagagata
     1501 acccatgtcc acttttaaag ttcaaattta ttcttgagtt ttcaaataaa accttgattt
     1561 ccactataac atctcctttc acatgaacgt gtattcatat attgtagcac attaagttct
     1621 attttttgtt gtaattaccg aaaaaaatat taatggacgc tttttacgct ttatttcgta
     1681 tgtggaagaa aatttcttag gatgtggcgg gggattgggg caaagctaaa ttccaattac
     1741 agtgatcatt taattagaca cctttaaata cccacacttt catcaacact ataatttagg
     1801 gaaagaaaag tgttggcatg atatagaaaa acctttaatt aacagcgttt cctaaaattg
     1861 gcacgcatct tgcttataga aaagcgtaag gataggagga atgaaaatgg caactataat
     1921 tcttgccagc catggggaat ttgctcaagg attaaagcaa acagctacaa tgattattgg
     1981 agatagtgta ccaattcatg ccttgtcagc atttagagat gaagatgaat ctattttggt
     2041 acaaataaaa aaacttttag ccacaataga tatagaagaa acctacattt taactgatat
     2101 tctaggtggt agtgtgaata atgatatgct cactattatt aaagagtatg agggattgca
     2161 attaattact ggaatgaact tacctttagt tatttctatt gcaacctata atggaaaaat
     2221 tggggaaagt gagctagaaa agcttattaa agagagtcaa caatcagtga ttgattgtag
     2281 aaaattatta gtagaacaac ttactggagg agatgaccta tgattaagtt agtacgtatt
     2341 gaccatcgtt tgttgcacgg acaggtagta ttttcttgga gtaaatcatt aaatatcaat
     2401 cgtattatta ttgccaataa agaggcagcg aatgatgaat tcaaaaaaat gtctttgaat
     2461 ttatctaaac cgactggcat taaattaaca attttttcag ttgaacagtt actagaaaaa
     2521 atgcctaaaa tcgaaacact taacgaaaat atcatgctta tttttggtaa tacaaaagaa
     2581 attcgtcaat tttgtgaagc ctatcctaaa gtacaagaaa tcaactatgg agggataatc
     2641 aaaaaagaag gttccaagca atttagtaat gccattttct tgaatgaatc tgagcaagaa
     2701 gatgccaaaa aattaaaagc tcttggcatt aaacaattta ttcaacaggt accaacttct
     2761 aaaaaagaag atttgaatac aatgttataa aggaggaatg aaaatgctcg ttcagtcaat
     2821 tggtttaggg ttaatcggtg ttttttgtat tttagactca aggcttttag gaagaatgaa
     2881 ttttgaacga ccgttgattg tcagtacgct tgttggtttg gtacttggag acatggaaaa
     2941 aggattaatt gttggagcct ctttggaatt gatgtcctta ggattagtaa atatcggagc
     3001 tgctgcacca cctgatatga atatggcatc aattattgct acagcctttg ccattttatc
     3061 aaatgcgaat gcagaagcag cattaacgat tgccattcca atttcagtgt taggtcaaat
     3121 actaggtatc ttaatgcgga cgattttgtc aaatttgact cacgttgcgg atcgctttat
     3181 cgaacaaggg aaatttagcc aagcaaggag aattcatata ctttatggct cgggattata
     3241 tgctctcatg tattttattc ctatttttct agcaatttat tttggaacga atcttgtttc
     3301 tcagattgtt gatgcgattc ctgagtggct aacaaatggc ttaacggttg caagtaaaat
     3361 tttaccagca tatgggtttg cgttgttatt acaaacgatg ttaacgaaaa aaatggtgcc
     3421 atttttatta cttggatttt taattactgc ttattctgga ttgggaatca ttggtattgc
     3481 agccttcgca tgtattgttg cgtttgtcat ggttcaagtt caaggtaaaa actcaaatgg
     3541 taaccaaaat gagccagatc cattagatga cttataagga ggtagattaa gtgaaagaga
     3601 aagatcaaaa tgcatggaaa tatcggcagt tcttatggcg ttcatgggca attcaagcct
     3661 catggaacta tgaacgacaa atgaacatgg gttttatgta cggaattgca ccaattctag
     3721 ataacattta caagaatcca gaagatgaag caatgaagaa agaagcttac gagcggcata
     3781 tgatgtacta caactgtacc cctcaaacaa gtgcatttgt gttagggtta actgcttcta
     3841 tggaggagca atactacgaa gaccaagaaa actttaatcc ggaatcaatc agtgcagtga
     3901 agacttctct gatgggaccg ctatctggtg ttggtgattc tttctttcaa ggaacgattc
     3961 gggtattagc ctttggacta ggaattaatt tagcccaaca aggtagtatt ttaggtccaa
     4021 ttctagcaat gttaatttcc tttgtacctt cctttttagt tacttactat ggtgggaaaa
     4081 ttggctataa catggggaat aaatacttgt ctaaactgta tcaagaaggt ttgatggata
     4141 aagtaatgta tgtttgctcc attgtggggt tgatggtcat tggttcgatg attgccagca
     4201 tgatcgggat tacaacacca attcaaatgg gtaaagcttt tgtcttacaa gatgtattag
     4261 atagtattat gccacaattt ttaccattag gattaacctt cttcatgtac tggttattgc
     4321 agaaaaaggt caaaacagga tggatgttgg caatctgttt agtcggagga attattttta
     4381 gtttgttagg tatcttacaa taagggaata accaataaat ttggttgaat aatataaata
     4441 aggaggaatt caattgatga attcaaaaga aattgttaaa gaaatcgtag cgaaaaaaga
     4501 ggtaaaatca atcgtatttg ttggctgtgg cgcttcaaaa gcggaccttt atcctgcaaa
     4561 gtattttcta gaccaaaatg cgaagcagtt acggattagt cattacacag ccagcgaatt
     4621 taactatgca acaccagcat ctgtagatga gtcaactgtc gttatttcag cttctttagg
     4681 aggtacgact ccagaaacag ttgaagctaa cgcagtagct aaagaaaaag gtgctacagt
     4741 tattagtcta acacgaagca ttgactcacc gttaacaaaa gatgcagatt atgtaattta
     4801 tcatcgattt gcagagaact atgctgctaa attagaaaaa acaggctact gtttagaatt
     4861 ggcagtagaa cttttacatc aagtagaagg ttatgaccat tatgaagcaa tgcaagaagg
     4921 atttgctact atctatgatc ttgcccaaaa aagtgcagaa tcagctcgta aagatgcgaa
     4981 gaaatttgct gaacgcttta aagatgaaaa agttatttat gtaatgagta gtggtgctac
     5041 acatgaagta gcatactcaa catcaatttg cttgatgatg gaaatgcaat ggattaattc
     5101 tggtaccttc cattcaggag aatttttcca cggaccattc gaaattgtgg ataaagacgt
     5161 accgtttata ttattaatga atgatggaaa aactagaaat atagatgctc gagcattaac
     5221 tttccttgaa cgctttgata cgaaaacaga agttgtagat gccttggatt atggcttgtc
     5281 ttcacatatt gctaaagaag tcttagacta ctttaaccca ttcgtgatta ctgcagtatt
     5341 tagagtttat gcagaagaat tgtcagaagc aagagaacat ccattaacta agagacgtta
     5401 tatgtggaaa ctaacttatt aaaaaataga tcaaactaaa taaggtggct tgagaaatta
     5461 agccaccttg ttttcttcaa tgtgatttta tacatatggt aaaataacta gtacatattg
     5521 tgaaagcagg tggcaagatg cagcgattaa atgactatct ttatcaacta acattgcttt
     5581 tgaaatctac gccttcagat aattattcag ctcgcagtat ttcgcaagct ttacagttga
     5641 atagaagtac aatcagtagc tacttaaatg aaggtgttcg tgaagggctt ttcataaaag
     5701 taaaaagtta cccggtttta tttttacata gaacagctct tgaagaacta catatcacac
     5761 tgaataactc cgaaatagaa tcaattgaaa gcttgttaac aatttctcaa aaacccgcat
     5821 tagatcaagt aattggctct aaaggaagtt tgaaagaagc aattgatcaa attaaaacgg
     5881 cagtcttata tcctggaaaa gggttgcctt tattattgat cggcgctagt ggatcaggga
     5941 aaacctttct agcgaataaa atatatgaat atgcagtaga agaaaaggtc attcacacat
     6001 cggcaccttt catagattat aattgtgcac aatatgtgag caatccagag ttactatcct
     6061 ctgctttatt tggctacacg aaaggagcat ttaccggagc aagccaggag catactggtt
     6121 tgctagaaaa agccgatgga ggagtattgt ttttagatga agtgcatcgc ttatctgaag
     6181 aagggcaaga gaaattattt acatttatgg atacgggtga attctctcct atgggggata
     6241 atagtattcg gaaaaaagcc gatgttcgat tagtctttgc aacaacagaa aatatatata
     6301 cgactttctt accaactttt cttaggagat tgccagttat tgttaatcta cctagatttc
     6361 aacaacgtcc gtcgtttgaa cgtctgtcat tgattgatga attttttgtt tctgagagta
     6421 aaattttagc taaggaacta tctgtttctg atgcattgat tcatttctta atgaatagtg
     6481 attttgaagg taacgtgggg aatattaaaa atattatcaa gtatgcttgc ggaaacgcat
     6541 atatccacca gaagagtcag caaacaattc aagttaaatt attggatatg cctttagagt
     6601 acagttataa attcaaggag caactaaata aacccagaaa gaaaagagca gatcgaatct
     6661 actatccaga agaaacacga caagtgcaac tagaaagaaa caatcagctt tttgttcaat
     6721 tttttgacca attacttact ggattctctg aagtgattga aaataacaag gatattaaac
     6781 tatttttaga agaaatggtt accaaggtaa cacaagtaat ggatacgctt atttttcaag
     6841 aggattacga aaaagagcag tctttatacg ccattctaaa ctatcatatt cgtcaaagta
     6901 ttgattttat gcgagaaagc tacgggtttg aacaagatgg taaccgggta gtcgctttgg
     6961 ccaactatct atatgtcaag gataatcaag tgatttttga aaatagtctt caagaaaaga
     7021 aaaccaaaga tcagcttaac gaattttttg ctaatgttct tgaaacccct tattggtatg
     7081 caaaaaaaat cttaagcctt ttatctgagt gtttagatca acctgtgtat gaagaggaca
     7141 ttttctttat tacgctatat ttttatagtt tgaatgtcac gaatgttaaa acaggtatta
     7201 agagtattgt gctagctcat ggttattcta cagctagtag tatggcgaat gttgccaacc
     7261 gtatgctgaa taagaacgtt tttcaagctt atgatatgcc tttagatatt acattagaga
     7321 aaattgaagc aaaattgatt caattccttg gagattatcg aacagattca ggcttgattt
     7381 tattggtaga tatgggttca ctaaaccaac taggcggttt attagtgaat catctaaatg
     7441 gaccactgtt aatcattgat catatcaata caccgttatt attggaagtc ggccaacaag
     7501 tattaaacaa taaaagtatc accgaaatag gagcagaaat tgaaacaagt cttactttca
     7561 aaaagcagtt actacttccc aagaaaaaga aaaagaaagc catcattact tgttgttata
     7621 cagggattgg tagtgcgacc cagattcagg agattttaac gaaatgcttg aaagatgcag
     7681 ttagagaatt agtaattatt ccgtatgatt acaaaaagct gttagaaaac aagaattttg
     7741 agactccttt tcaattatac gatgtcatca tgattgtagg tacagaagat cccaaaatta
     7801 atcagattcc atatattgga ttagatcgcc tgattaatgg ggaagcaatt ggcgaatttg
     7861 ttcattactt ggaaaaagag ctgactattg aagtgaacga tttacaaaaa gaattaattt
     7921 tcaatttttc aattagtaaa attgtcgaaa atttaaccat tttggatcca gataaggtgc
     7981 tatcttttgt acaaaaagca attaaagaat tatcgaagtt attaaatttt gatttttcta
     8041 ataatcagct gtttttgttg taccttcact gtagttgtat gattgaacga attgtacgta
     8101 aagaaagtgt ggatgagcaa gaggacattg aagaatatat gcaagcaaat ggagaaataa
     8161 tggataaaat tcatagcgct ttttttgaga ttgaaaggga atacacaatt gaaataccag
     8221 atttagagtt gcgattattg agcgatattg ttcaaggata ggagtgaatt cgaatgacaa
     8281 tgggagtaat ttttgatatg gatggggtac tcatagacag cgagacattt tattttgaga
     8341 gaagaatgaa cttctttcaa gaactggaga tagatccagg atcaaaccag attgatgatt
     8401 atgtggggaa aacagaagct gcaatatggg aaacattagt tcctgaggac aaatcattaa
     8461 gagaggaact ttatccgaaa tatgtagatt atcgaaaaac gcatccaatt gatttcgcta
     8521 aagatttacg aaaagaagtg aaaccgttac ttgacttcct caagaagcgg gatataccat
     8581 tggcactggc atcttcttct ccagttgttg agatagagag tatgctcatt caatgtgaac
     8641 ttactgaata tttttccttt gttataagtg gtgagtcatt aaaacaaagc aaaccacatc
     8701 ccgaaatata tttaaaagcg aaacagcaat tagactgtga cttctttatt gcagtagagg
     8761 attcaacgat tggtattaaa tcagcaaagg cagcaaatat ctatacggtg gcactaaaac
     8821 aaccaatgca aatcgatcag agtgaagcag acatacaaat tgaaagtcta caagaactgg
     8881 aacaaattgt tttagaaaaa ggttagtgaa ttaatggaaa taaggctata tatctgtgct
     8941 agctattttg actaaccaaa aaaacttgct ataattcagg tgtagtaaaa ggtgctggta
     9001 acacctttta cgaaaatgct tcggcagtga taatgggatt ggctttttaa gttttttatt
     9061 ttgttcgcaa tccctaaggt catgtgaatg atcctaggga ttgctagaat attttgcaac
     9121 attctatcta tttcagaacg taaccttccc cagttcattg ggattcatta gcatacaacc
     9181 aacatttcaa aaagaaattg cactacacca ttattagcta ttttgactaa ccccaaaaaa
     9241 cttgctataa ttcaggtgta ggtaaaaggt gctggtatca ccttttacga aactgcttcg
     9301 gcagtggtaa tgagtgtggc ttaaaggact agtcctttaa gctttttact ttgttcgcaa
     9361 tctctaagat caagttaatg atcgataaga ttgctagaat attttgcaac attctatttc
     9421 agaacgtaac ctttcccatt ccactgtgat tcattggcaa ccctcctcac aaccaacgtt
     9481 tcaaaagaaa ttgtaaagtt gttggggtac cacgaccgaa caatactaca cctgaatgat
     9541 agtaagttgt ctcattaagt atagcatatc ctctttttgg gtaaatagaa atcggttaca
     9601 ttttttatcc aagaccaagt tttttcattt ggtcttattt gctataccca aacaattccg
     9661 cagtatagtc gacggtattg attttatcag gtgagtaata gaaacttgtt ttccttcccc
     9721 aaaacattac ctataaagga gaaaaaatgg tatcgtaatt atataggtat aggatgaaat
     9781 ttttttctaa gaggagggtt gttgatgaaa aatttacagt taaaatttat tgttgatcca
     9841 tcgattgtac gctggtttcg gattatcaat caatttgagc gcaatcaact tgccacaaaa
     9901 ggagaattag caaagagtaa tcatgtatct gaacgaacca ttcaaacaga tgtcaataaa
     9961 atgaaggact acttttctga aagtgttcaa tttgtttcaa cgaaaagtgg gtattctttt
    10021 cagaaggaaa gaccggttct ttttctcaag cagaaggagc aactactgga gaacgaaatt
    10081 ttatttgagt tgttagggaa tgtcttttat ggcgaattgg aagatatgac ggagctaatt
    10141 gatcgttttc atttatctga agcgactttt cgtcggtatt tgaagcagat tcaaccagtt
    10201 ttggaagaat acggcttaga attgagtctt tatcctcttg atttaaaagg agaagaggca
    10261 aatattcgca agttttttaa agacttctat tatgaagggg agatgacgcc ccatactctt
    10321 gttccaccaa gggatcttca tgaacttgtg caagctacat tctatgataa atttgagtat
    10381 ttctcgattg gaacagggac ttctcctgct gaattttatt atagtttgta tattgcgatt
    10441 gagcgtgtga gacaaggaaa aacgatagcc attcctcaag gtctgattga gcgagtaatt
    10501 gctagtgaga attttcgctt aatgtattcc ttgaaagaag cgattcaaac aaattatcaa
    10561 gttgctttat cagaagagga gttttgttgg ttgtatt
//
LOCUS       DACEOM010000091        10559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEOM010000091 DACEOM010000000
VERSION     DACEOM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.095"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(11..295)
                     /locus_tag="IW781_002557"
     CDS             complement(11..295)
                     /locus_tag="IW781_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1183534.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(285..1019)
                     /locus_tag="IW781_002558"
     CDS             complement(285..1019)
                     /locus_tag="IW781_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1183535.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            1148..1771
                     /locus_tag="IW781_002559"
     CDS             1148..1771
                     /locus_tag="IW781_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1183536.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            1963..3084
                     /locus_tag="IW781_002560"
     CDS             1963..3084
                     /locus_tag="IW781_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1183537.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            3091..6219
                     /locus_tag="IW781_002561"
     CDS             3091..6219
                     /locus_tag="IW781_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1183538.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            6238..9432
                     /locus_tag="IW781_002562"
     CDS             6238..9432
                     /locus_tag="IW781_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1183539.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(9578..10285)
                     /locus_tag="IW781_002563"
     CDS             complement(9578..10285)
                     /locus_tag="IW781_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1183540.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         3680 a         1853 c         2193 g         2833 t
ORIGIN      
        1 tttttaacat tcatatagat tcaggaagca aaacggttga gtgtgtctgt aaataacgtt
       61 ctttttgttt tcgggtgagt tttttgaatg cagcttctgc ttttgttgct tcgcttcttg
      121 aagaaaatac ttctgcatgg atcatcttaa caggtcttct tttttgaaga cgagtatatt
      181 tagcaccagt accattgtta tgttcctcta atcgtctaga aagatcggtt gtatatccac
      241 cataaaatgt ctgatcttta cagaataata cgtaaaaata atgttcattt gccatagagc
      301 atctcacgaa tctctgcagt gtattctcca tccatattgt aagtgatcaa tggtggagcg
      361 actttgaatc cgtctaattt gccttctttg attccttcaa ctaacaaaat atttgcctct
      421 tttccaggtt ttgggtaaac aaattggatc cttttaggtg caattcgata gtcttccatg
      481 acctgaatga tttctaagaa ccgatcagga cgatggacca tagtaaagcg tccatttgtt
      541 tttagtagct tacttgaaat atcgatcact tcttctaacg tcgtatgaat ctcatgacgg
      601 gcgattgcca ggtgtttatt agggttcgtt ttatttgttg gcagcccttt gaaataaggt
      661 ggattacaga cgactaagtc acaagaatta tgtcgaacga cagataagct gtctttcaga
      721 tcgatggtgt ggacagtcat ttgttcattt aaatggttaa gcgcgatact acgtcttgcc
      781 atgtctgcta gacgaggttg aagttcgata gcgtcgatag ccgcctgggt tcgtttactt
      841 aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa caatcttccc ttttttaggt
      901 acacgggaaa agtgagcaag cagcacagca tctaaagaaa aagaaaacac tttggaactt
      961 tggataatct tgatatcttc tgcgtataat tggtcgattc gttcgtcttc gtgaagcatg
     1021 gattccctcc aaaatgaaaa aaatagtcgt ctgtattata ctatttttat taaaaagaac
     1081 caaggtggct tgtcaaacct ctatttttta tgcatactat gaccataaga agggaaagga
     1141 agagactatg ttttatcgat ttatgcgcgg ggtagttcga gtactattag ccttagttaa
     1201 tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa gatgagaatt acatactagt
     1261 tgctccccat cgtacttggt gggacccatt atatctggcg gtagctgcag cacccaagga
     1321 attcagcttt atggcaaaag aagaactgtt caaaaatcca gttctgcgat ttatattaac
     1381 tcgatccaat gcttttccag ttaaaagaga aaatccagga ccaagcgtca ttaaaacacc
     1441 tgtaaagatt ttgaaatcga caaatttgag tttgatcatg tttcccagtg ggacaagaca
     1501 tgctacagaa cttaaaggcg gaatggcatt gatttccaga atgtctaaag cgaaaatcgt
     1561 gccagctgta tatcaaggtc cgttgacact tggggatctg ttcaagcgtc agagagtgac
     1621 agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag aaaacggata aagaaggtat
     1681 ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa ttggacaaag aagtgaaccc
     1741 tgatttcaaa tatgaagtca aaaaaaaata aaatgataag tttatgcgtg ggcggtcttt
     1801 tgaatctact gtcctgttca gttcagactt tttcaatgaa aacgagggag agacgtcaag
     1861 tctgccctct ttttgcgttc caatatgtaa aatagaagac aggtgtcatg taatggatca
     1921 aagtgttata ataattagaa ggacattttt tgaggtgatt gaatgcgttt tttacataca
     1981 gccgattggc atatcggaaa aaaactacaa ggttatgact tgattgaaga ccagcgtcat
     2041 gtactatctt ctatactaga tatcgcgaaa gaagaaaaag tagaggcaat cgtgattgcc
     2101 ggagacttat atgataggag tgtcccagct gttgatgccg tagaattata caatgaattg
     2161 atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg ctatatcagg aaatcacgat
     2221 agcagtacga gactttcagc gggtacgcca tggttcagtt attcgaacta ttttttgcga
     2281 acacagctag cagaagcatt tgaaccagtc gagtttaaaa acgtacaatt tttctttctt
     2341 ccttactttg aaccaattgc agcgagatta tattttgaag atgacaatat ccgaacgatc
     2401 aagcaagcgg tagccaaggt gatagataaa atgcaggagc tatttgattc agaaaaaaaa
     2461 caagtattga ttagccattt ttttgtaatg ggaagtgtaa aatctgattc ggaaacgaaa
     2521 atcgaagtag gcgggttgga ttcgatccct ggtgaactac ttacaccgtt tgattatgta
     2581 gcactaggac atttgcataa tcaagctgct ttgagacagg ataatgctcg ctatagcggt
     2641 tccccattga aattctcttt atctgagatc aaccaaaaaa aaggcgtatg gatcgttgac
     2701 ctgacaacag gcctggagtt gaattttagg gagatcgaac ccctgcatga tattgaagaa
     2761 ataacagcga gtttcaaaga attgctggat cctgcctttt atcagtccgt caatcgggag
     2821 aattacatct atatctatct cactgataga acgatcatcc cgaatatgat gaaccagctt
     2881 cggcaaatat atcctcgtat tttggggatt gaacgggcaa acggtcgagc agaacaaaca
     2941 aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa aattagcaga agcatttttt
     3001 agtgaaatga cggaagagca gctaactgca aaccaagaaa aatggctcgg agaaacgttg
     3061 caagaaatca tggaaatgga atgatcaaga atgaaaccga aaaaattaca acttaaaaat
     3121 tttggtcctt ttatcaatga gaccgtagat ttttcacgtt tgacagaagc accgcttttt
     3181 ttgatcagcg ggaagacagg agctggcaaa acgacgattt ttgatgggat caccttcgca
     3241 ctttttggtg aaacttccgg aagacttcgc tctggaaaag agatgcgttc tttgtttgct
     3301 acaccagacg aagaaacaag cgtgactttt tcttttgaac atcagcagat gagctatgaa
     3361 atcgaacgaa aaccagaaca agttttagca aaacgaaaag gaaacggaac aaaaaaacag
     3421 gcagccaaag taacgttgac catttttgat gggaaaggaa aagaaataaa acaaatcaca
     3481 aaacgtacag aagcagatca gttgatcaga gaactgatga atctcgatgc gaaacagttt
     3541 tctcaaatcg ttttactacc ccaaggagaa ttccgtaatt ttttggtttc atcaagcagt
     3601 gataaagaga ctgttttacg tcatttattc ggtactcaat ttttccaaca gttcaatgag
     3661 cgattaaaag aaaaagcaaa acaacagcaa caaaaattgg atcatttaga gcaagagctg
     3721 catcttctgc aaaaaaggtt tgttttagtc aaagaagagg aaccagcagc agggtttgaa
     3781 gccgttttag cccaatggac aaaccatcag gcaatactcg aaaaacaaat cacggaagaa
     3841 aaagaagctc taacggaact tcaggaaaaa caaaagcaac tagaaaattc ctactatcgt
     3901 tttgaaagct tgaaaaaaag ttatgaagaa aaaacgatat taataaacaa gcaaaaggaa
     3961 ttagctgctc aaaaagacga aatagaggaa aagaaacgat ggattgggta ttatgaattt
     4021 acgcaacgat tagtcgaacc aatccagcgc ctacaagaaa taaaagttga aaaaatagaa
     4081 ctgtcacaaa aaagtagtga gcaaacaaag aaactagcga tgactacgca gaattatgaa
     4141 gagtggcaaa aaaatgaagc aaaccgcgta cgccgacaaa caaaaatcaa agaagctgct
     4201 caaaaactgc aaaacaaaca gatgatggtg ccaattgtcg aatccttacg tagtaaaaaa
     4261 caagacgcag aacaattaac aatttcattg gataaagaag aattgaatgc cgagaagctg
     4321 tatcaaaatc tcgctatcta tcaggaaaga actcgaattc ttcaagaaaa tttactggat
     4381 caggaaaaac tgcaagaaga tcgtcttgct tttggacagt taaaatatct agatgaaaag
     4441 cagcaagaaa tagaaaaaca aaaagcaaaa gacactgggt cgctcgcaca gataagagaa
     4501 gaactcaaaa aagcaactga agaaataaca aaaaatagtc agctattgaa agaacagaca
     4561 gaacaagcag aacagctgaa aagccaatgg gcaaagcagc aaattgctcg tcttcagctt
     4621 tttttgcgtc caggggagcc atgtcttgtt tgtggatcaa cggaacaccc catacaacaa
     4681 ttaaaacacg aacagccaaa taaggaagat atcttaaaag tagaacaaca actggcagaa
     4741 gctgagtctg ctgtagaaag aactcagcaa atcattgcca aaagtgaagc acatgaaaat
     4801 aatcttcaac aacaattatc agatttagaa caaaaaatca tggcaacgaa tatacaattg
     4861 gctgaacaag aagaacactt gcaacaggct ttgaaaaagg catttcctga gataacagaa
     4921 caaaaaacaa aaacaactct tgtagtaatc gaacaatctt tagatagacg aaaaagcaag
     4981 atggccaatg caagaataga gctggaagaa ataagacaac aagcagaaaa gctcgaacaa
     5041 caattagctg tctacaaaca gcagcagaca gaaaaaaata atcagctcca gcaactgcaa
     5101 ggagaaatca aaagtttaga acaacaattg caagatacgg atagtaactg tttagaagag
     5161 gagatacgac gacttgaaaa acagttagaa gaagactcga atcaactagc tgttgagcag
     5221 aaagcaggag tgcaattgtt gcaagatctt catacgcttc ggacacagct tgatcttcaa
     5281 catgaacaaa tagaaaaaat agatgaaaaa atagaaaagg aacaagaaag aatagctaaa
     5341 aagttagaaa gccagtcgta tttttcaaca attgaagaaa tctgtgcatt tgcacaacag
     5401 caagcacaat ataaagaaaa ccaacgtaca gttacttctt atcatgaaga gcaattagtg
     5461 acagctgacc ggctgcgtca attagaaaat atccagctaa ctgagacatt cccagataca
     5521 gaagagatca gccaacagtt agaagaaata agacgtatga tcgaaataaa gcaagaaaag
     5581 atatacggtt tacaagaaca aaaaattagt aatcaaaaaa tctacgatga atgtctttcg
     5641 atttatcaca caagccagac gaaattagat gagcttacgc aattacagca gctttcgcaa
     5701 acattcaacg gagaaaatcc taagaagact agtttggaac ggtatgtgct tcaggtttat
     5761 cttcaagaag tgttacaagt agcaaatgat catctccaac gtttaacgaa aaatcgttat
     5821 caatttgagc tagcggatac cattggcagt tatcgaggga aaaccggatt agaaatcaat
     5881 atctatgatg atcaggcagg tatgtctcga agtgcgcata ccctgtctgg cggtgagagt
     5941 tttattgcag cactttcttt agcattagct cttgccgatg tgatccagac acaagctggt
     6001 ggtgtagcaa tcgatgcatt gtttattgat gaaggatttg ggtcattaga tgaagaagct
     6061 ttggaaatgg caatggaagc tttagaaacg atcgaaagtg aaggaagaat gatcggcatc
     6121 attagtcatg taagagaatt gaaagaacga gtattacaac aaataagaat tgatacagaa
     6181 ggtagcggac aaagcaaaat acgctatcaa cttggataaa aagggagatt ggtaactatg
     6241 aaatggagta ttccagaaaa agtcattgca cgtggacgtc aatacttgca agaaggccga
     6301 gtgttatcgg ttgtccctga ccaagaagaa aacgtgtggc atgcagaagt cttgggcagt
     6361 gaattgtata tggttgattt ggatggtaca gcaaaagaga tagattactg ccaatgtccc
     6421 tattgggagg aacataaata ttgtaagcat acagttgctg tggaacttta tttacggtcg
     6481 aaaaatctat ctcgtatcat gaaaaaagac cagccaatgc tagaaccggt aaaagcaaca
     6541 agtgagggag aaatcattgc aaaaggtttt tctcgtctaa aaacacaaaa caatcctcaa
     6601 gcggtgcaac ctttgattat tgaataccaa gtagaaacga ttgagactaa tcagtatcac
     6661 ccagagcttt ctattttggc actttacttg cgtatcggtt cattggcatc accgaaaaaa
     6721 acatatatcg taaaaaacat ctatgatttt ttacaagctt acacagaaaa agaaacttac
     6781 actgttaata aacagtacca gtttcgtcta gataaacggg cgtttcaaaa agaagatcaa
     6841 aaaatactca cgcatcttgc aggaagtgct cagacgcatc aattattagg tacgaacggc
     6901 ttacaagtca aaggaaaact agataaaaga tatctgcttc tcccagtaga acaagcccgc
     6961 ttcctgttag agcagatgaa tcagactaca cgtttgcatc tgcagataaa tgaacaaaac
     7021 gatcaaggaa tacaattttc cgatcaaaac aaacctttat cattcaaagt ggaaaaattt
     7081 ggagaagatt atttgctgca agcattgaat gattttgatt ttttctttat gcattatcag
     7141 tggggagtta tccaagggac attttattct ctgacgaaat atcaacaaac ggtttatcaa
     7201 acttggaaac aattgattcg ccgattggag aaaccagaag tagtgttcaa gaaaaaagaa
     7261 ttaccgtctt tgtttaaaga gattatccct ctgctgtcag agatcggcga cgtagctatt
     7321 gcccctgcag tagaagagga agtagcagat atccccgtcg aatttatttt ctttttccga
     7381 aaagcaaaag gaaaaatcca agctcgagtc gatttcgtct atggcgaagt catctattcg
     7441 actgacccga agcatgaggt ctcgacagaa caggcttttc aggtgttacg tgacacagta
     7501 gaagagaaac gagtacttga tctattcaga atgtatcgct accagaaaaa tgaaactggt
     7561 tatgaacgtc cattgccaag cggggaagaa ctatatgcct ttttccgcac agaactatct
     7621 gtcttccgtc agtttggtga agtcagactt ggaagaaaat tgcgagagtt gtatttagat
     7681 gcgcataaac atcaaccgaa aatcgaagta accgagaccg gttcatggct cgatattcga
     7741 ttcgatgtga cagggataga ggagcaagaa atcgatcatg tcctgcaaag cttgctgaga
     7801 aatgatgctt tttatacatt agaaaatggt caggttcttt cttttgattc agaagaattt
     7861 caacaaacaa gccaagtgtt gcaacaatta cgggaatcga tacgtacaga agaaggaacg
     7921 atccatgtac ctaaaaatca aggattgatc attcaaaatc aattggaaaa aagcaatgct
     7981 acttttagtg aatcgttcca aacgatggta caagatctca ttcatccaga aagataccag
     8041 gcacagcttc ccaaaggatt gaatgcaacg atgagagatt atcaaaagca aggttttcga
     8101 tggttgaaga tgttaggtca ttatcagttt ggcggtattt tagccgatga aatgggactt
     8161 gggaaaacac tacagacaat tgccttttta ctttcggaaa aagaagaacg gaaaagcttc
     8221 tctgctttga tcgtagcgcc tgcgagctta atttacaact ggcaggcaga ggtgagaaaa
     8281 ttcgcccctt ctttaagtat ccaagtaatc aatgggaata aaaaagaacg tgaagaatta
     8341 ctggctaaag acacagatat ccgagtgact tcttatgcca gcttaagaca ggatctagct
     8401 aattatcagt cgcaaaaaat tgattatttg attcttgatg aggcacaaat ggtcaaaaat
     8461 agcagtacga aaactgcaca agcattgaga gaattagctg tacctcagcg gtttgcactg
     8521 agtgggacac caatcgagaa taatttagaa gaattgtggt cattgtttgc aacgattatg
     8581 ccaggatttt tcccgactaa aacaaaattc agggaacttg ccgcagaaga aattgcgcaa
     8641 atgattcgac cgtttatttt acggcgagat aaacaaactg tgttgaaaga cttgccggaa
     8701 aaaacagaga tgaatctata ttctgcactt acagaagaac aaaaaactgt ttatttagct
     8761 tacttgcgtc aaatgcgtga agaaatcaca tcaatggatt ctgaagcatt caagaaaaac
     8821 cggatcggga tacttgctgg tctgacaaga ctgcgacaaa tttgctgcga tcctcgtctg
     8881 tttattgaag attaccaagg cggttccggc aagctcgaac aagtaaaaga tctactggta
     8941 gctgccaaag agaacaaacg aagagttttg ctgttttctc aatttacagg gatgctgact
     9001 atcttgcaag aagagttagc cgagctaggg atctcgacat tttatttaag aggcagtacg
     9061 aaaccacaag atcggttaag tatggtagac gcatttaatg ctggcgaaaa agatgttttt
     9121 ctgatttcct taaaggctgg gggaactgga ttgaatctca cgggagcaga tacggtcatc
     9181 ttgtatgacc tttggtggaa tcctgccatt gaagaacaag ctgctggacg tgcacatcgg
     9241 atcggacaaa aaaatgtggt agaagtgtgg cgtatgatct ctgaaggaac gattgaggaa
     9301 cgcatggatt cactccagca agaaaaacgc gaactgttcc aaaaagtgat ccaaggtaat
     9361 gaagaacagt tgacgaaact gactgaagaa gacatccgca tgattttaag tgtaggagaa
     9421 atcgaggagt aagagactcg aaaaagcgtt cagtaatggg taagaaagcc tttctaaaga
     9481 agcgattgtt tatttgattt aaaaaatagc aaggaggctg ggattccatt ttatcccagc
     9541 ttccttgcta tcattttttt atttctattt ttaaaggtta tacaaagatt gtcttcattg
     9601 tctctaacag ctgatcaaaa gaagaaatct cgatatcgca aattggagtc tgttcaattt
     9661 tgcgttctcg atggttgaac caaagcgctt gccagttagc actttttgca cctagaacat
     9721 cattgtcgta attatcgcca acgtataagg tctcttctgg aagcatatga aattctttct
     9781 cagctagctg aaaaatctct ttttctggtt tttggaaacc tgttgcttga gaaatgagca
     9841 tattgcggga aggaatccaa ttgttcaatt gaagttgctt cagttttttt gtctgatggt
     9901 cagtcggccc atttgtgata attcctaatg ggacattctt ttccttcaag aaatcgaggg
     9961 tttttttgac ctcctcatgc aagcagatat tatcaagttc ttcctcgtaa attttttgaa
    10021 ataagaggcc atcttcttcc gtaatatgag ggtagtctaa atcttttagt gattggctga
    10081 tccgatgtgc tcgcatatat tctagtgtcc aatctcctgc cattactttt gggaagtttt
    10141 catcactatg atggcggaac cggatatata gtggatgcat atctgcatct gaaacaagcg
    10201 gaacgactct tttgaccgcg tttcgaaatg gctgctgctg atcatacatc gtatcatcaa
    10261 catcaaacac aactgattta atcaaaattt tacacgcctt tcggttttca ttgatttttt
    10321 cacaaatcct tgactattgt aacagaaaaa attgtctaaa aaagctgata aataaaggtt
    10381 tttcttacta tatttctatt tccttgacag aataaggttt ttatatttca gtttttttca
    10441 ctaaaaatcg tgaaacacgt caataagttt gatgaacaaa ttaatcatta tggtagaagt
    10501 cgattgattt attttaatgc aatctctgtg gaattttcga aagatttatt tttttaata
//
LOCUS       DACEOM010000092        10155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEOM010000092 DACEOM010000000
VERSION     DACEOM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.096"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(559..1599)
                     /locus_tag="IW781_002564"
     CDS             complement(559..1599)
                     /locus_tag="IW781_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1183541.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="IW781_002565"
     CDS             complement(1948..2274)
                     /locus_tag="IW781_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183542.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3049)
                     /locus_tag="IW781_002566"
     CDS             complement(2261..3049)
                     /locus_tag="IW781_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1183543.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="IW781_002567"
     CDS             complement(3417..3707)
                     /locus_tag="IW781_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1183544.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3843..4022)
                     /locus_tag="IW781_002568"
     CDS             complement(3843..4022)
                     /locus_tag="IW781_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183545.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4019..4369)
                     /locus_tag="IW781_002569"
     CDS             complement(4019..4369)
                     /locus_tag="IW781_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183546.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4362..5687)
                     /locus_tag="IW781_002570"
     CDS             complement(4362..5687)
                     /locus_tag="IW781_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1183547.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6137..6751
                     /locus_tag="IW781_002571"
     CDS             6137..6751
                     /locus_tag="IW781_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1183548.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6916..7920)
                     /locus_tag="IW781_002572"
     CDS             complement(6916..7920)
                     /locus_tag="IW781_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183549.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(8379..8636)
                     /locus_tag="IW781_002573"
     CDS             complement(8379..8636)
                     /locus_tag="IW781_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1183550.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8629..8898)
                     /locus_tag="IW781_002574"
     CDS             complement(8629..8898)
                     /locus_tag="IW781_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1183551.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(9029..9208)
                     /locus_tag="IW781_002575"
     CDS             complement(9029..9208)
                     /locus_tag="IW781_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183552.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(9589..>10155)
                     /locus_tag="IW781_002576"
     CDS             complement(9589..>10155)
                     /locus_tag="IW781_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183553.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3101 a         1854 c         1458 g         3742 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat ccgtaaaata
     3841 ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc tgcagcttct
     3901 acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc ataaccccaa
     3961 atctcttctg atagctgatc aaaggttata tactttagct catattgctg gataatgttc
     4021 attctaatcg actccattta ttatcctggt aaaattcgat atgacgtatt tcgtcgtagt
     4081 gaattttgtc ttttccaacg tagatcccca gctcatcact acctaagatc ttgccaatga
     4141 tatcttctgg atagtatcct tcagcgtttc gttcttcttt ttgaatagat actgatctat
     4201 tttttatgac tgcagcgttc aatacttccg caatctcttc agtcgtcatt tgttcttttg
     4261 ctaaattttg actggttcgt tgttcattgt cggcatcaat ttcttcagta tgatcagata
     4321 aataaaaccc tgcccacttt aaacgtccac gatcattgta atcaatcatt tttaattccc
     4381 ccatttccgc ccgcatgacc accaacaagt gtgcttctag taatgcttct agcgccttct
     4441 aacattgaag aagcatgtac aatcgacgta aaaccatatt tttgccgaat cttgtcaata
     4501 attttatcta aattttcttc gtcaatttgc ttttgtggat cactaaataa atccagctga
     4561 agcgaatcgg tgtacacaag cttgctatag gttattccaa catgccttac ttcttgtcct
     4621 tcataatact tatcaaacaa gaaaagacaa taattctgca attcctttgt attatctgtg
     4681 atagggatct tcatctggtg tgaaaaccct ggtctcgttt ctaagatcga cgtgccaatg
     4741 tgtaaatgga cacaacctgt tttacagtta tgctgcctaa tgcgtatcgc tacttgttcc
     4801 gccatttctt ttaccaccag ctcgatttca tttcttctag tataatctcg tggtaacact
     4861 tgtgaatttc catatgattt ttcctttgtc ggttctggtg ggattgctat atctgttcga
     4921 tcgatcccat tagcatggaa gtacagctgt aagccaatca cacctagtct attcttaatc
     4981 gtataaggat tccagttagc aagatctcta atgctcataa tccccatctg attgagtctt
     5041 tttttcattc gagagccaat tccccaaaag tccgtcattt caggaatatt ccatactttt
     5101 tcaggaacgt tttcgtacgt ccattccgca acaaatcctt cattatgctt tgcttcgttg
     5161 tctaaggcta gtttagccaa aaggggatta tctccgacac ctactgtagc aatcaatcct
     5221 agctcttctt taatattctc ctggatcatt tgagccagct tctttctccg ttgacttcgt
     5281 gttccttcag tcgtaaaaag attcagtgat cgggtcactt ttaagattga ttcatcgatc
     5341 gagtaaatca gtagatcttc atcagccaca tatcttctga aaatattatt tacctgcata
     5401 tttcgcttga tatataggtt catacgtggt ggaacaacgt gtagtgtttt aggaaatggt
     5461 tgtggtaagt cacgtggtct actcacattt gtaataccat accgcttttt tgcttcagga
     5521 gaagaagcta atatcaatcc tgaaccagta ttatcagctc gactcataac aacaagttct
     5581 gttgttaatg gatctaaatt tctttctata cattcgacac ttgcgtaaaa gcttttcata
     5641 tcgattaaaa agtaatcatt tactggctct tttgaataat ccatcataaa taacactcca
     5701 ttctttcaca ttatacaaac atatgttcga tattgtaaag aagaaatagc taaatacata
     5761 aaaaaagaag cataggtatg ctatcaccct gcttcttctt ttgatttgta tgatcaattt
     5821 attctatcac tttactaatt aatttgaaaa tctaataaaa actctcactc taaaagagtg
     5881 agagactgca aattagagta cggacttttt tggagttccg ttctttaata ttaacaaatg
     5941 aataaaatcc ttacaacatt tgagaataag ttatcagtag acacttacaa aaaattctca
     6001 tactatttcc tccatcatta gtttttgtct atataaggtt taccctaaat agacactggt
     6061 ataacattga aaaatatggc ttcctcgtgt atgattaggg tgtattttaa atagttgcag
     6121 aaagtaggta aaagttttga gagttgcata catacgtgtc tcatcgatcg atcaaaatga
     6181 acaacgccag attgaagaaa tgaaaaaatt cggtgcagag agaattttca tcgagaaaca
     6241 atctggcgca acgattactc atcgacctgt tttccaagaa gcattagatt acgtccgaga
     6301 tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattatg acgagatcat
     6361 cgcttcagtc aattacttga agaagaaaaa tgtgaagctg atcattacta gtttaccgat
     6421 catggctgaa gcaattggca atcctctcct ggataaattc attaaggatc tgatcatcca
     6481 aattttggcc atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc aggcacaagg
     6541 aattaagatc gcaaaagcaa acggcgtcta caaagggcgg cccaaattat acagtgcaga
     6601 cgcaaaagat ccccaacgtc gccttgttta caaaagcatc gttgaagatc ttaaaaatgg
     6661 tgttgcgatt gctaagattg ctaaagacta caatgttacg agacaaaccg tgtatagaat
     6721 aaaaaaagac agcatggtca atgacaaata gttgatcatg ctgcttttat tatcttctca
     6781 tctttaattt cctacatata attactatct ataaaattaa gtcttactca gaacagataa
     6841 agcataaaat tccaacaaac cattttttac ttaactaaat aaagagtctg ttaatataat
     6901 cttcacttat ttacgttaat cccctactgt atttgttggt tccttatcta tattttctac
     6961 aaaatcctta ttatcttgtg atttagcttc gtataattct ttgtatgtcg acacattggt
     7021 tatactaatt cctatagcta gttcttgaga ctctactttt ctctctagct cctttgacaa
     7081 agtgatttga gatttatttg gttccgttgg agtcaattct atagattctt cataaaaatc
     7141 tctatacata aagctatcat taattttata aaaaaaatta aatgttttta gcactgccat
     7201 ataacattca aaacattctt ctatagttgg tttacttcct tgaggagaat gaaccaattt
     7261 attacgtttt tctatgaaat ttttaatttc ctttcttatt tctttcggaa actcaatatt
     7321 aaagtaccct ttaaacatgc tccctaataa tttgtctaca gggggagatt gtaaattctc
     7381 aaatattaga gttaaatcag gtttctcttg ttgataaaat tcctttaccc ctatttcaca
     7441 ggctgtaatt atcgaaataa acctatctct agtatttgat atatttttac ttcttttaag
     7501 gagtataaga gaaaagggga tactttcgtg tccatgcatg taattaacta attgttgagc
     7561 ttcagaagtt atatctctaa ttatatagtt aacggaatta ggaagtgtct ttaactctaa
     7621 tgaaatttct atccaattac tatcttctaa tctgtatatg ctcgaagtat aatttgattc
     7681 gtcagcaagc atcgctacat cccaaacact gatcataaaa tcttttaaac gttcaatcat
     7741 ttcagcaata ttgctaaaat cagctaattt ttctttagaa taaaaaaaaa catttatatc
     7801 agaatcaata aattctattt tttttattac tatatcgtca tcattatctt gaccatttat
     7861 gtttattgtt ccctgagtgg ggagtacttc tttcaaattc aagttaaatt tatattccat
     7921 tacttctcct ccaatatata aaaattactt aactgtatcc aaatttaata tgtaaataaa
     7981 tcattaattt ttagctaaat tctatgagaa gtttattttt gttgtttttg ctaattaact
     8041 aaaactagta caaaaaattt aaaatagatt attaaaccta ttgcttattc tattcagtca
     8101 tatatataag cgtcatcctt tagtaactaa catcccacac tacaatagta ttataacata
     8161 tctattcaac ttcaaattgt tgaaatttaa gccatttata tttacttttc ctcaacttta
     8221 gaaatgattt tactaacctt atatccgtaa tacgcgtaac aaacacattc attaataaca
     8281 atcaacacta agctatatta gctttttaaa tgactagggt aaataaaata aggatcaaaa
     8341 acttttaagt ttctgaccct ttccttactt ccgattggtt aatagtgatc ttttgcagaa
     8401 taaataaata tcgtttcatt ttcaactcta tatatcagtc tatgttcatc tgtaattctt
     8461 ctggaccatt ttccagataa atcatgcttt aatggctcag gttttcctaa tccagcaaag
     8521 ggggaacgat cgatatcttt tattaacttg ttaatctttt ttatattgct tttgtttcct
     8581 tgctcatgcc aataaagata atcatcccaa gcatcatcag accaagcctt aatcatcaga
     8641 ttcaacctcg attaagtcat gtgttttaaa tgcacctttg gagaattgtt catctcctcg
     8701 acgaattttt tccatgacgt aattattaga aagtgttctc aacgtttctt gcatagaatc
     8761 ataatctctt tttgataata caacaactgt atcttctaca tctttacttg ttacaataag
     8821 tgtttcagca tcctcattaa cttgtttcat ataactacgt aaattttggc ggaaatttga
     8881 ataagctact gcttccattc ctttcacccc ttatttattt ctataaaaca attgtacatt
     8941 atattgtaca attaagcaaa tgattaatat tctaacttct tataattaaa agtgctatca
     9001 cttttagttt ttaggataaa aagtgacgtc actttttgag tgctggtacc tcaaatcctg
     9061 ctttttcttg ggcaatcaat aaatctaacg caatacacat aatcctggat tcacttttgc
     9121 cgtacttctg ctttagttgc tgcaattctt tttgtctctt ttcatccaac gtaacgggta
     9181 accgtttatt tcctattgca attggcatta gtactctctc ctttcatcga aataacccta
     9241 tgaaaaatat ggctgcaatc atcttgaata gtagactcac aagggctaat acaaagcgcc
     9301 aaaagatcaa acaaaatacc agtattgcca aaaatactac gaagtttaaa tgcacagctg
     9361 cagcatggcg cataagtatt tcgccaacaa ataggactaa caatagagga atgattttat
     9421 tcatatggat ctccttcttt ctcttaggtt atcgggtgtt gcccgcttat ttcgcaccac
     9481 ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa gaaccattcc cttagttact
     9541 aggttttttg tccttatttc acacattctc acttttcggt ataaatgatt attgcctttt
     9601 tttcctttca aatcgttgat atggcattcg tttacggtca agttcatttt ggatttcttc
     9661 tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa gtcgtgtacc agtcgtcgcc
     9721 tgggttatct acttcttcta catattccct caaaaatgct tcgggtacag cgagttcata
     9781 atccattccc attaacacga tagattccgt tccacacgtc aaaaatctag taaatacaag
     9841 gtaataatcg tctatcgtcg catctttgct gtttagctct tccatttctt cttccattaa
     9901 ttccatgctc tcaaaaattg aataatattc tgattctgag taatagtgtt tcctgcaaat
     9961 cgtgtagctg acaaagtttt cgaccagctc actcctttct aggtaaacat tcatgatgta
    10021 tttttctgtt tctagtcgta gaagctccgt tccttttttt accgtttcag gacgacgttt
    10081 atacacgtct aaacgattat ggctgactgt gaccttcgtt tgactcgttt cactttccaa
    10141 atacaattgt tcttc
//
LOCUS       DACEOM010000093         9992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEOM010000093 DACEOM010000000
VERSION     DACEOM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.097"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            201..680
                     /locus_tag="IW781_002577"
     CDS             201..680
                     /locus_tag="IW781_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183554.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="IW781_002578"
     CDS             713..2941
                     /locus_tag="IW781_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1183555.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="IW781_002579"
     CDS             3070..4713
                     /locus_tag="IW781_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1183556.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="IW781_002580"
     CDS             complement(4786..5331)
                     /locus_tag="IW781_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1183557.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="IW781_002581"
     CDS             complement(5354..5839)
                     /locus_tag="IW781_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1183558.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="IW781_002582"
     CDS             complement(5829..6641)
                     /locus_tag="IW781_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1183559.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="IW781_002583"
     CDS             complement(6780..8312)
                     /locus_tag="IW781_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1183560.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="IW781_002584"
     CDS             complement(8421..9452)
                     /locus_tag="IW781_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1183561.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3325 a         1844 c         1920 g         2903 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgcg tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa tagttggtac
     9841 ttcattactt tacatgaaga tttattctat ggtaggagca attatattga gaattaatcc
     9901 ttttttatat aagttgtttg cttattgatg gattggataa attaaaccat cacaacgagt
     9961 ggaaattata tgaaattttt cataaaaatt ca
//
LOCUS       DACEOM010000094         9535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEOM010000094 DACEOM010000000
VERSION     DACEOM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.098"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            66..1187
                     /locus_tag="IW781_002585"
     CDS             66..1187
                     /locus_tag="IW781_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1183562.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(1269..3041)
                     /locus_tag="IW781_002586"
     CDS             complement(1269..3041)
                     /locus_tag="IW781_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183563.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3044..4756)
                     /locus_tag="IW781_002587"
     CDS             complement(3044..4756)
                     /locus_tag="IW781_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183564.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4871..5539
                     /locus_tag="IW781_002588"
     CDS             4871..5539
                     /locus_tag="IW781_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1183565.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5606..6394)
                     /locus_tag="IW781_002589"
     CDS             complement(5606..6394)
                     /locus_tag="IW781_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1183566.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6477..7949)
                     /locus_tag="IW781_002590"
     CDS             complement(6477..7949)
                     /locus_tag="IW781_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1183567.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8079..9272)
                     /locus_tag="IW781_002591"
     CDS             complement(8079..9272)
                     /locus_tag="IW781_002591"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1183568.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
BASE COUNT         3083 a         1993 c         1554 g         2905 t
ORIGIN      
        1 aaaaagcctt cgggcttttt cttttaacaa ttaataggaa cgtatattcg aaaggaatag
       61 aaataatgtg gttagaaaaa ataaatgaca aaaaatttaa gtacaccgaa cgttatacgg
      121 atcccttaac tgaaaaaaag agaaaagtgt ccgttacttt atctagcaat tcacgccaag
      181 cttggaatca agctaactta cttttgaatg aaaaaatcgc agaaaaaata aaacgaaatg
      241 aagaattacc actaactttt ggagaattaa aaactaaatg ggatgaaaaa tacaagccaa
      301 ctgtaaaaga aagttcctat cgaacaactc aggtttatct atcattaatt tcaaaatata
      361 taaaagatga tgtacttgta aaaaacgtta atagtaatct tatacaagat atgctagatt
      421 actactattt tgaaaaaaat ttatcatata attatgtaat acatttaaaa acttttacag
      481 gaatgatttt taaattcgcc aaccgtcgat atggattaaa atataatccc gtaaatgggg
      541 tttctttagc aagaaaacct aagacaaacg aagaaataaa aaaagaaaaa gatggttatt
      601 tatcaaaaga agaagttcaa atgattgcta gtaaacaaaa aagttcacat caacagtcac
      661 gttattcttt aattactaaa tttttgtttt taactggact acgttatggt gaattgatat
      721 ctttgactga atctgattat gatggtaata aaattacggt ttctggtact tatgattacg
      781 agttaaaaat taaaacaaca actaaaaata caggttctta tagaaccatt gaattatcaa
      841 gtaatgctaa agaaatcata gatcaattaa ttgaagaaaa caaattaatt aagaataata
      901 aagataaata cattttcatc tctaaaaatg gtaacccgat atctattcaa gcatataatc
      961 aatctttaag agcagtatcc aaagaattaa acatagataa aaaagtttct tctcacatgt
     1021 taaggcattc ccacatttct ctacttacag aattaggaat cccattaaaa gccattatgg
     1081 atcgtgtagg acatgaagat tcaaaaacaa cattaaagat ttatactcat acaactaaaa
     1141 atatgcaaaa tcaactagta gaaaaactag gaaaaataga aatttaaatt gccccttttc
     1201 tgccccttat aaaaacaaac aacataaaat atataaaaag aaccccgtta taacgaggtt
     1261 cttttaattt aactttcaaa tacaaattga ttatgataaa gttccgcata aaatccattt
     1321 tctgctaata actcgtgatg actgccttct tcaatgactt ccccatcacg caagacaacg
     1381 attctatctg cattcaagat tgttttcagc cggtgagcaa tcacaaagct ggttcttcct
     1441 ttgattgctt catccattgc tttttggatc cttgcttctg ttaccgtgtc tacattactt
     1501 gttgcttcat ccaaaatcaa taaagatgga tttgtgatga tcgttcgagc aatactgatc
     1561 aactgctttt gacctgtact gaataagtta ttttcttcgg atatctctgt atcataacct
     1621 ttttctaacg tcatgatgaa gtcatgaata ttcgcttgtt ttgctgcatt gatgacttct
     1681 tcatctgaag cgtccggttt gccgaatgtg atattgtctt tgatcgttcc ggagaacaat
     1741 acagattctt gcaaaacgat tcctacatgt ctacgtaatg catcaagatc ataatctcgg
     1801 atatctattc catcgatgac tactgaacca tcattgacat cgtaaaaacg gtttagcaga
     1861 ttcatgatcg tcgttttccc cgatcctgtt ggaccaacaa gagcaaccat ttctcctttg
     1921 tccacattga tcgagacatc tttcaagact agtttttctg gagaataacc aaaatctacg
     1981 tgctgcaacg caagaccttt ttcaacacca tcaaatgctt taccgttttc tggtttgact
     2041 tcatcttttt catcaaatat ttcattcaat cgtcttgccc ctgtaatcgc taattggatc
     2101 atgctgtatc cagatgaaat ctgcatcagt ggttggtaat attgttgtga atactggacg
     2161 aatgtcacaa ctaagcctaa tgccaccgaa cgttccaaat caccatttaa tgctaaccag
     2221 ccgccgaaaa agataactat cgctgtattg accagtgaca ttccttgcat catcggaaat
     2281 agtagaccag aataagcttg tcctttgaag gttgcttcgc gaacactttg attatgctct
     2341 aagaatcctt caatcgtttc ttcttgtaag ccatttgtga tgatgacacg ttgtccgctg
     2401 attttttcat ccatataacc atttagtttc cccacttcat cttgctgaag atccacatat
     2461 ttccgtgctt tgcggataat caagacagct atcaaaacgg ctactggagt ggaagcaatt
     2521 gtagcccaag ctaactctac attttggcgg aacatcatga tcaagatccc gacaagtaaa
     2581 acagcattcg tgatgacttg aagcaaagct tggttcaaac tattttgaat attatctaag
     2641 tcactggtaa aacgacttaa aatttcaccg tcttgatgag aatcaaagaa acggatcgtc
     2701 aatttttcca atttgttgaa tagaccgata cgcattcggt tcgttgactt ccctactact
     2761 tgtgtgaaca aaatactata gataaagttg gctgcactag ctagtacata aaacagcaag
     2821 agtttccata ttacagaaac aaatttgctt ttatcgtcta cgccttgcat caagcctcct
     2881 acgtaattag ctaattcttg aaaagcttcc ccgatgtatt gaggtgcctt tacttgtaga
     2941 taggtagcaa tgatcactgt gataaagata aagaaaaagg agagtttgta ccgcttcaaa
     3001 tagtgataga aaaatttact tgcttttatt agatcagtca tacttactcc tccgttcctt
     3061 tttgagtttc aaagatttcc tgatagacac ggtttccagc taccaattcc tcatgggttc
     3121 cttcgccgac taatctgcct tcatccaata ctaagatgcg gtctgctttg acaacagaag
     3181 caattttttg tgcgatgaca atcgtcgttg ttcccttcaa atctcgatcg agtgcttctc
     3241 ggaccagctt ttcagagcga gcatccagtg cactcgtact gtcatctagg atcaagatct
     3301 ttggattacc gataacgcca cgagtaatgg ataaacgttg cttttgacca ccagaaaagt
     3361 tactgctacg ttcttctatt ggtgcttcgt aacgatcaga taacttttcg atgaattctt
     3421 ttgcttgagc aatagaagaa gcatgttcca tctctttttc tgttgcattc tttttccctt
     3481 ggcgcaagtt ttgagcaatc gttcctgaga ataaaattgc tttttgcaaa acaaaggaaa
     3541 ccgtttgtct taaactcttt tcatttactt gacgaagatc tacaccacct acttcaactt
     3601 tgccttctgt cggatcaaat agacgaggga tcaattgtgc tagtgttgac ttcccggcac
     3661 cagtcgcccc gacgatacca accatttctc caggtttgat agaaaagctg atatctttaa
     3721 gcgtatctgt atcctcacca ggataacgaa aagaaacatg gtcaaaagtg acagaacccg
     3781 tcaactcttg ttctggaacg tctaaataag taagatctgg ttcagcatct aaaatttccg
     3841 caatccgctt cagcgatacg gctgctcttg aagtcatcat catcatcatt ccgccaataa
     3901 tgattgccat cataatctgc atcaagtagt tcatgaatga agcaattcca ccaattaacg
     3961 taggatcatc tttaacaaga tcgctcacaa aaaagatcgc cccaaccaca gccaaattgg
     4021 cagccagcat gaaggacggg atcatcacag aaaataatgt accgactatg atgttgtgtt
     4081 ttgttaagtc ctcacttact ttgctgaatg acttcaattg gttttcttct tgtacaaaag
     4141 atttcactac acgaatccct agtaagtttt cttttgcaat gctattcaca cgatcaatca
     4201 gtttttgaat gatcatgaag tgcttgccca tccttgtaaa tgaaagtgcc gtaatcagaa
     4261 ataccgtaac gataagcgct acgatgatcc accataattg cggcatcgtg atcatagcta
     4321 aaatgaaggc accaataaat aagaatggaa ttcgaaaaag tgattgcaaa gaaatcatca
     4381 ctaaattttg gatttgcgtg atatcatttg ttagacgaac aacaagattt ccagcagaga
     4441 actgttcgat atttccaaat gaaaatgtct ggatctttcg aaatgcttct tctcggatat
     4501 ctgcacttac gccttgagct acttttgcag agaaaatcgt gttcgttaca cctgcggcta
     4561 atcctagcaa agccagactg atcaggtaaa taccaattgt tttcatttca tcattatctt
     4621 ctgtaataat agcttccaat acttgctgta gtaacttagg ttgccataat gctgctgcaa
     4681 ccatgactgc tacggacagc aaggaaataa aaacagctac tttatatttt ttcgcatgtc
     4741 gtaagattaa tttcattcgt tgattacacc cctttgttta aattgacagg actaatttaa
     4801 cactgtaaaa ttagaccgac taacaattta ccatgtttaa aaagataatg caatacacga
     4861 ggttgataaa atggaaataa agctttctca agcaatcatc attcaggccg cctttgatat
     4921 cctggctgaa acacaagctc tttcgcagct ttccatgcga aacatcgcaa acagaattca
     4981 tgttcaagca cctgctttat attggtattt caaaaacaaa caacagctct tgcaaaaaat
     5041 ggctgaaaca atggaagaag aattagcgct tccagatcca actttgcctt ggaatgaaca
     5101 gttgctgcag tatatggaaa attactatga cctttataca cgtttccctt gtggggcaga
     5161 attagaaatc aatactgtgc ccgcttttcc gagtcgatta gaacatttgg agcaaatgat
     5221 ccaattactt gttcgtgaag gattttcagt cacccaaagt catcaggcca ttgcctctct
     5281 ccacaattta ttgattggac agttaatgga tcagcagcat gaacagcagc ttcgacaaaa
     5341 aattatggac ggaaatgaat ttttgaaaga aacagttcta tttatgcgta attatgtagt
     5401 tgaaaatcaa ttagagggaa ttaaagaaag tatgcagtcc catgcagaat tgaatgaaaa
     5461 agcactcttt ctaacgagtg cacaaaccta tattgaaggc ttatgccaac aaaataaaag
     5521 aagagaaaac gagcagtaaa aaagctccta tagagatcac tatgtttgtg atctctatag
     5581 gagctatctt tttaagactc tttttttatt tttcttccat tagccacgcc aatgcacgtt
     5641 gaatccattg gtcccagaag atccagttat gatcgccagg tccttgttca aaggtgaccg
     5701 ccaagccttg ttcctgcata gcagatacca tttcctgatt tgcctgataa agaaaatcag
     5761 attccccaca agccatataa aatcttggaa ctggttttcc gctttttact agttctttca
     5821 ataagtattg cggatcattt tttgaacctt cgatttgatc caatggtcca aagatccctt
     5881 cccaaaaaga tgctttacga accatcaaaa gatcatccat tcgatttcct acagaaacag
     5941 cgcctgaaag agagattact gctgcgaaat tttctgattt tgcaaggcct agttttaacg
     6001 cgccatatcc gcccatcgat aatccagcgg catatgtttt ttctcttttt cttgttaatt
     6061 gcgggaataa ttcatgacaa atctcaggta gctcttctgc aatgaatgtc caatacttca
     6121 tgtcataaac tgtatctgtg taccagccaa gatcagtaga tggcatgatg acagctaatc
     6181 cgtagtttgc tacataacgt tcaatcgacg tacgtctttc ccatacacta tgattgccac
     6241 ccattccatg aagtaaatat aaaacaggta tatccgttgt ctcttgctct gaactggtgc
     6301 caatcacctt ttttgttgtt tgcggtagta tcacatttag attgacttcc atttctaaca
     6361 cattcgaata aatatttgct tgaataaatg ccatattttc gccttcctta gtaagaagtt
     6421 atttttgaat aactttaaat ttgaatgatt cggctacgta catatcttaa ccgaaactac
     6481 tgttttattt taccactttt ttcatgaacg aaaagcgtta agaccaaagc aatgatcacg
     6541 agtaacaagc caactagata aacggaatgt aacccatcat aaagaatttt tcttaatggt
     6601 aacaaaatat ccgcatcaat atatttagcc gtttgtggat tcaccagttt attcatgata
     6661 tctgcatcat ttgttaaatt tgctgctgca agttctcgtt tagttacccc atttatcaat
     6721 aaaccaaaaa tagaaaccat gactgtttga ccaattgttc gaactaacgt attaaaagaa
     6781 gttgctacac caagctggtc acgagataca ctattttgtg cactgacagt cgaggtagtc
     6841 atggtcacac ctaagcctat accgatgacc gttgatatag caaaaaacca taaatagtta
     6901 cttctgaaag gcataagata caaccaagcg cccccaatag ctgcgattag tagtccaatt
     6961 ctcaagaccc aatgagtttt catttgagtc atccacttgc ttgcaaggaa agaaccgacc
     7021 atccagacta gtgataatgg tgctaatacc agcccaccaa ttgctgcatt cagccctaat
     7081 acaccttgca tccacatagg gatataaaca tcgattccca tcaaaaaacc actagccaat
     7141 gctgcagcca agttgaccac aacaaatagt ggatttttga ataaatgcat catgatgaca
     7201 ggatctttag ctcttttttc ggcaaaaaca aaaataccta acattacgat actacctagc
     7261 agcaaagcag aaacaagcgg actcattcct tcgtctccta ataattggaa ccctaacagc
     7321 aatgagacaa gcatggccat caaactaaaa ctaccaaaaa tatccattgg tttagactca
     7381 tgtatgattt ttggttcatt taaatatagc caaatcaaaa taattaatag aattccgatc
     7441 ggcacattga taaaaaagat ccagtgccag ctgatcgttt caacgatgaa tcctcccgca
     7501 agcggtccaa aaacactggc aatcccccag gctgaactat tcaaacctaa aacttttgct
     7561 cgcttttcaa tagaataaag atcgccgatg atcgtcaaag ctactggcat cattgcccca
     7621 gcacccatcc cttgaatcgc acgagcagca atcaacccaa tcatgctttg agataaaccg
     7681 caaaataacg aaccgataat aaacaataat gtcccaaaaa taaagattgg tttccgtccc
     7741 actttatccg caagttttcc gtaaatcgga gtaagcatag cgtttgtcag caaataaatc
     7801 gaaaataccc agttcatgat ttccattcca tgtaatgaac cgacaatcgt tggcatagcg
     7861 gttgttacga ttgtaccttc aatagccgtc atgaacgttg caatgaaaat actcactgtg
     7921 accaaagtaa catttgtttc ttttttcatc ttattcctcc ctttcattat gacaggatct
     7981 aaaaatagat aaataattac tatctttttc aagaggttaa ctgccataaa aaaagctgga
     8041 acaaccacca cttggcgatc gttccagctg aatataactt attttgctaa acttgctttc
     8101 aacgcatcta ctttgtccgt atgttcccaa ggcaaatcta catctgtacg accaaaatga
     8161 ccgtaagcag ctgtttgttt gtaaatcgga cgacgcaaat caagcatctc gatgattcct
     8221 gctggccgaa gatcgaagtt ttctctcact gcagcaatca gttcttcttc cggaaccgtt
     8281 ccagtaccga aagtattgat tgagatcgaa acaggttgag caacaccaat cgcataagct
     8341 agctgtactt ctgctttgcg agcaagccct gctgcaacaa tgtttttagc aatataacgt
     8401 gcagcatagc tggcagaacg gtcaactttt gtcgcatctt taccagaaaa agcaccgcca
     8461 ccatgacgag cataaccgcc atacgtatca acaatgattt ttcttcctgt taatccggca
     8521 tccccttgag gaccaccaat gacaaatcgg ccagtcggat tgatataata tttcgtttga
     8581 tcatctaata attcagctgg aataacttcc ttcactactt tttcgatgac atcatgacga
     8641 attgtttcat tatctactgc atcatcatgc tgtgttgaaa taacgattgt atctacgcgt
     8701 tccggttgtc cttgatcatc atattcaacc gttacttgag attttgcatc tggacgtaaa
     8761 tacgttaatt catttgattt acgcaaatct gccaagcgtc gtaccagacg atgactcaaa
     8821 gcgattggta aaggcatcaa ttctggtgtt tcatctacag caaacccaaa cattaaacct
     8881 tggtcaccag caccaatctc atctaatacg tcttttttat cttcatcccg gatctcaagt
     8941 gcttcatcaa ctccttgagc aatatcagga gattgttcat caatcgcaac caatacagct
     9001 gctgtatctc catcaaatcc gaattttgca cgtgtataac caatttcttt tattgtttcc
     9061 cgtacgactt tttgaatatc tacatatgcc gtcgtagata tttctccaaa tactaaaaca
     9121 agaccagttg ttacagacgt ttcacacgct actcgtgctg ttggatcttg ttttaaaatt
     9181 gcatccaaga ttgcatcact gatttggtca gcaattttat ctggatgtcc ttcagaaaca
     9241 gattctgatg taaataagtg tctttctacc attataatga ttccccctag ttaaattcgg
     9301 ttacaaggca tctccgtcct gcggatcctt tcgggaactt gcaacgaacc aagtatagca
     9361 gataaaacag ttttctgctc gtattttttc atttttttgc ttaaaaaata taaaaatatt
     9421 ttacattaga actgtccaaa aaagcttagc acaatcatcc ataaagataa ctctgctaag
     9481 ctttttaaaa tcattctttc agataaaaag aaaaagatac tcaaaaagta tcttc
//
LOCUS       DACEOM010000095         9472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEOM010000095 DACEOM010000000
VERSION     DACEOM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.099"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW781_002592"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW781_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1183569.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="IW781_002593"
     CDS             1873..4539
                     /locus_tag="IW781_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1183570.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="IW781_002594"
     CDS             4903..5655
                     /locus_tag="IW781_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1183571.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="IW781_002595"
     CDS             complement(5677..6831)
                     /locus_tag="IW781_002595"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1183572.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="IW781_002596"
     CDS             7016..9457
                     /locus_tag="IW781_002596"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1183573.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3059 a         1707 c         2025 g         2681 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct tt
//
LOCUS       DACEOM010000096         9444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEOM010000096 DACEOM010000000
VERSION     DACEOM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.100"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            59..205
                     /locus_tag="IW781_002597"
                     /pseudo
     CDS             59..205
                     /locus_tag="IW781_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="IW781_002598"
     CDS             282..611
                     /locus_tag="IW781_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1183574.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            601..888
                     /locus_tag="IW781_002599"
     CDS             601..888
                     /locus_tag="IW781_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1183575.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1174..2031
                     /locus_tag="IW781_002600"
     CDS             1174..2031
                     /locus_tag="IW781_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1183576.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            2032..2580
                     /locus_tag="IW781_002601"
     CDS             2032..2580
                     /locus_tag="IW781_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183577.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2840..3473
                     /locus_tag="IW781_002602"
                     /pseudo
     CDS             2840..3473
                     /locus_tag="IW781_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(3518..4467)
                     /locus_tag="IW781_002603"
                     /pseudo
     CDS             complement(3518..4467)
                     /locus_tag="IW781_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            5069..6913
                     /locus_tag="IW781_002604"
     CDS             5069..6913
                     /locus_tag="IW781_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1183578.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            6969..8435
                     /locus_tag="IW781_002605"
     CDS             6969..8435
                     /locus_tag="IW781_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1183579.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            8472..9314
                     /locus_tag="IW781_002606"
     CDS             8472..9314
                     /locus_tag="IW781_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1183580.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         3246 a         1444 c         1906 g         2848 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag
      661 aagccattga aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat
      721 aagggtgaac aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg
      781 ggcacaacat atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta
      841 tgtggaacgc atgaaaactt ttatgaacag ttgaaaaaat taatctagtg atttgttgtc
      901 gttcttaaga aggacggctt cttttttatc gaaaatctga ttcgtctaca tgactaaagt
      961 cgcgagtatt ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata
     1021 aatcaatata tttcagcttt tccttattta tgtactacct tttacttatt aattataagg
     1081 tagtacatat atatcttttt tttggtagta actaaaaata aatatgttat aatagtatca
     1141 aatgaatatt gctaaaagaa aagaggtagg aacgtggtag cgaagaaata tgtaatcggt
     1201 aattttaaag gtggcgtagg taaaagtacg tgcgcgcaaa tgttagggtt tgaatcagca
     1261 gttaatgaag gaaaaagaac cttgataatt gacttggata tgcaaggaaa tacttctgat
     1321 gtgatgaact taacgcatat gaacttcact aacgaagaag gaggcggaga aggtgaaccg
     1381 attgtttacg aaaatactgt gactgacgtg ttgataaaca atcttccacc taaagaggct
     1441 gtctataaag taatcaataa cctctatata cttccagcag acatgtcctt tgaaatgtat
     1501 gatgattgga tcaaagacca atataatgat tcattatccc aattcaaata tatgaaagaa
     1561 aagctagaac cattatttga tgagtttgac gtcatctatt tagatgtccc accttctatt
     1621 tctgtatata gtaaatccgc gatgtacatt gcggattggg caatcgtcgt cttacagacc
     1681 caagtaaaat cgatgagaaa tgcgatgcaa tatcttgagt atatggactt ttttgtgaaa
     1741 gaatttgata caaatttata tgttgctggt gttattcctt tcatgttgga aagcggtgac
     1801 gcagtagatc aggaaatgta taaacaagcc caagagattt atggggaaca tttgattaaa
     1861 aatgttgtac tcaaaaatgc gcgcttaaaa cgatacgacg gatcaggtat tacgatggag
     1921 aagacaaaga aaggtctctt aaaacaatgg gatcgcaaaa gccacgaatt atttatcagt
     1981 attttgaaag aattggaaga acacgagaat tggtatgcgg aggtggagta attggctgac
     2041 atcaaaggac ttgatatttt tgggaacaac aaaggactcg ccaaaataaa aaaagaagca
     2101 gaagcaaaga aaaagcagca agcaattaca ccatctgaaa ataagcaatt agaagtgcac
     2161 caagttcaag aaataaaaac ttcctccaat gttgaaagac agagtagagg aagaacaaaa
     2221 aaagttggcg caccaatcag aaagtttgat aaggtttatt ccaccaagca tcctttaaaa
     2281 ctgtcgactt tgttaaatgc gacttccaga gttttagtag aaaagtatga gactaatatg
     2341 acaagagatg aattgcttag aaaagcttta gatgagtata ttaaacagaa tcttacgcaa
     2401 gaagataaac aagatttgtt taatgacgtg gtgaaagaac tagacttgtt cagagaaaaa
     2461 tatccaattg ttccagaatt agatgatcgt ggagaaataa taagaactac cagtcagata
     2521 aaacaagata ccgaagactc actgcgcaaa gggtggggaa tgaataataa agttagataa
     2581 taatcaaaga agaactaagt aactaccttt aaacggtagt tacttagttc ttctttgatt
     2641 tgcgtgaaat agaggggatt tatttttctg gttacgtgat taaaaaatcc aaaaaatgag
     2701 cataggaacg gttatgattt tagggtgtgt tttaatgaga tattgtgatt tcattaataa
     2761 gtgtatgata atctgttgtt gagttagtaa ataagtaata gagctaactc taaaataaaa
     2821 ctattaggag gagatgtaga tgaaaaagtt taaaaagaaa atatatttag gattggttgt
     2881 tggggtgagt atgtttgggt tcatggctca tactaaacaa gtaactgcag atactaacca
     2941 aaaagaaagg attatgagcc aattagatga ctctcaaata gagtatgtac ccaatgagct
     3001 aattgtcagt ttaaaggcta cacaaagaat ttcaacagag aaaaataaac cgcaaaattt
     3061 attgggtgta aaagttatgg tggaaaaaga tttgactctt aggacaaatg aaagcgatta
     3121 taataagcga gatcaagtat tattagtgaa attgaacgaa gaaattagtg tggaagaggc
     3181 agtaagaaca tttaatgctt ctgatatggt caattatgca gagccaaact atgtggttga
     3241 actagagccg ttgaatgatg ttgaggagcc aaatacaact gattctttag gaaatcgata
     3301 acgatcctaa ttggagtaaa caagctaatt taagagagat tagcatgcca tccgcttggg
     3361 aagaaaccaa gggttctaag gatgtgaagg tagcggtcat tgattcagga attgattatc
     3421 aacatgaaga tctttctgga aatgtagatg aaagtttggg atacgatttt ttttactttt
     3481 ttcaagaaaa taataattca gaatattctg actatattta tgcaacacta gtgactttag
     3541 gagaaacatc aatagccttg ctaaagtttt tgctttcgct aaagagagta ttttcgataa
     3601 tttgtttttt tctaaaatac ctcgttcctc aatatttggg atatggatat gtatagggat
     3661 tcttcgcata aaggtttcca gaaaatcctc tttgccttct gtagtcgcaa aaactaaacg
     3721 tacattggag gcgtgatgga tagtttcacc gactctagaa taagttccgc catccatata
     3781 aacaaatagt ttctcttgcc cttcaggagg caatctgtgt acttcgtcta aaaacagtac
     3841 tccaccgtct gcatcttgca acaatccatt tcgatcttca tctgcgccgg taaaagcacc
     3901 tttaacatag ccaaatagta tccccgacaa taattcagga ttattatggt attgcgcaca
     3961 attaagtact ttaaaaggag cattctcatg aataacgttc acttttttca taaactgata
     4021 agcagtatga acaaaaaaac tttttccaac tccagtatct ccagtgatca tcataggaag
     4081 ccctcttcca ggatagaaga cagcagtttt catttgttcc accgcatcgg ataagctcat
     4141 atcataccca atcatctttt gtagaatgga atctgtagaa ttgggctcca agtctgtttt
     4201 taattcagat atactttcgt atacatcttt taccggaatc aaaaagtgct cttcaaagcg
     4261 ctctttgatg gcaaataaca ctggacgagt attgatttta aatagaacgt tctttgcaac
     4321 taagtcattg gcaagtttac tggcatttgt tcttcccgtt cttaaaaatt gcgacaggtc
     4381 atttgtgtca aaaaagcact cttctaaatt actgatgcca atttcttcac attttgtgtt
     4441 tgcataagtc actatatcat ttttcatctt ataaacagtc ctttctaaac ttctatccct
     4501 taaataaata gcaaaaaaaa ggaagcaata tcgatatgct gtcctcttaa tttaataaag
     4561 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
     4621 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
     4681 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
     4741 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
     4801 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
     4861 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
     4921 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
     4981 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
     5041 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
     5101 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
     5161 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
     5221 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
     5281 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
     5341 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
     5401 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
     5461 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
     5521 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     5581 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     5641 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     5701 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     5761 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     5821 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     5881 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     5941 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     6001 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     6061 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     6121 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     6181 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     6241 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     6301 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     6361 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     6421 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     6481 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     6541 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     6601 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     6661 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     6721 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     6781 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     6841 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     6901 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     6961 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     7021 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     7081 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     7141 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     7201 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     7261 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     7321 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     7381 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     7441 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     7501 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     7561 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     7621 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     7681 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     7741 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     7801 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     7861 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     7921 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     7981 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     8041 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     8101 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     8161 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     8221 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     8281 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     8341 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     8401 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     8461 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     8521 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     8581 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     8641 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     8701 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     8761 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     8821 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     8881 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     8941 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     9001 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     9061 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     9121 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     9181 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     9241 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     9301 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     9361 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     9421 ttaacgtaag ataataaaaa atct
//
LOCUS       DACEOM010000097         9419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEOM010000097 DACEOM010000000
VERSION     DACEOM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.101"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            278..787
                     /locus_tag="IW781_002607"
     CDS             278..787
                     /locus_tag="IW781_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1183581.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IW781_002608"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IW781_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1183582.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IW781_002609"
     CDS             2322..3302
                     /locus_tag="IW781_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1183583.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4662
                     /locus_tag="IW781_002610"
                     /pseudo
     CDS             3424..4662
                     /locus_tag="IW781_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
     gene            4728..5501
                     /locus_tag="IW781_002611"
     CDS             4728..5501
                     /locus_tag="IW781_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1183584.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            5494..6285
                     /locus_tag="IW781_002612"
     CDS             5494..6285
                     /locus_tag="IW781_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1183585.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            6295..6786
                     /locus_tag="IW781_002613"
     CDS             6295..6786
                     /locus_tag="IW781_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1183586.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            6804..7214
                     /locus_tag="IW781_002614"
     CDS             6804..7214
                     /locus_tag="IW781_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1183587.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            7218..8618
                     /locus_tag="IW781_002615"
     CDS             7218..8618
                     /locus_tag="IW781_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1183588.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            8641..9015
                     /locus_tag="IW781_002616"
     CDS             8641..9015
                     /locus_tag="IW781_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1183589.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         3285 a         1344 c         1933 g         2857 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agttggataa tgttagttgg
     4681 cacagtaatt gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc
     4741 aaacattttt agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa
     4801 tcggaacctc ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc
     4861 aaactggact ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg
     4921 gtggtgcatc agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct
     4981 caactaacca ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg
     5041 tacaactaga tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat
     5101 tcgcacaaaa agaagattat caaaaattag agtggatgca atatttggca tcaattaata
     5161 caagctttgt atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg
     5221 gaagtgcaat caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac
     5281 tattacctgc tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg
     5341 cctatttgtt agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt
     5401 cgttacttgg cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag
     5461 tagcaccagc tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac
     5521 aaacaagtaa ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg
     5581 gaagggttgg gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat
     5641 cctgaaggaa tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat
     5701 acagcgcctt acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct
     5761 ttggaagcag taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat
     5821 agtgtatttg tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg
     5881 gaaggtagtt ttgttggaat tattttgtgg atactggcat caattttatt aaggattact
     5941 gcttatcctt tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa
     6001 acaaaattaa agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca
     6061 ctcattgcca cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca
     6121 atgaccggaa atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta
     6181 gcaattgtgt attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc
     6241 ttagtagttt ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat
     6301 gaaaaaatga aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta
     6361 gcaacacaat ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta
     6421 gccaatgatg acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact
     6481 tcaataatta caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa
     6541 aaagttctta ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta
     6601 ccaataacaa gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt
     6661 ccgacgataa atattactga agaagagaga gaggctttta aagaactaat tagtgatgga
     6721 gttgaagtga cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt
     6781 tattaagaac agaggatgag tggatggagt ttataattac atctcatgga aatattgcca
     6841 ttggatttgt agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa
     6901 cacttggaga tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag
     6961 taaaaaatca agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat
     7021 ttgctaaaag aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag
     7081 gaattttgat tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg
     7141 aaaatctaag aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg
     7201 aagaggatga gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg
     7261 attgcatgcc tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa
     7321 atcctaaacc tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga
     7381 aatttgaaga atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca
     7441 tttacgctgg aagtattgtc caaggaatca atgagtattt agctttgtat actggacata
     7501 atcgcaaagc taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag
     7561 atttaattca ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg
     7621 ataagaatga ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt
     7681 tgatactagg agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt
     7741 ttgaatccga agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat
     7801 ttaacatgat tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt
     7861 cagagtattg tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt
     7921 ggaaatcacc agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg
     7981 gaactaatga aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg
     8041 atcaaaataa ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag
     8101 acaaaactct tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc
     8161 cattttcaag ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta
     8221 aaatcgaaga ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt
     8281 ttgctttaaa attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat
     8341 tgattaataa tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg
     8401 ataaggggct gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa
     8461 ttgttgatga tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt
     8521 atgataaagt cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata
     8581 aactagacta ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa
     8641 gtgaaattaa atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa
     8701 atcttttatg aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa
     8761 gtcggctttt tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag
     8821 tgtagaggag ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat
     8881 tcaattcaag atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg
     8941 gaaaatggaa tccgttactt taatttctac ggaccaaatc atgagattat agagataagc
     9001 caaatgaaga aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc
     9061 attttttggt atcagcattt caatagttta atgagaattt actatttcaa attaattcaa
     9121 agtgacagta ttttttactt atttggtttc aaattttgta atccttccta tttattggaa
     9181 tacactccac attttgtata caagtcatat catgttttag caaagctctt accaagcaaa
     9241 gttataatga tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca
     9301 ggacagccaa aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc
     9361 tgtatgcctt tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactccta
//
LOCUS       DACEOM010000098         9191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEOM010000098 DACEOM010000000
VERSION     DACEOM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.102"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(185..853)
                     /locus_tag="IW781_002617"
     CDS             complement(185..853)
                     /locus_tag="IW781_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1183590.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            987..1274
                     /locus_tag="IW781_002618"
     CDS             987..1274
                     /locus_tag="IW781_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183591.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1690..3228
                     /locus_tag="IW781_002619"
     CDS             1690..3228
                     /locus_tag="IW781_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1183592.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3225..4190
                     /locus_tag="IW781_002620"
     CDS             3225..4190
                     /locus_tag="IW781_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183593.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4177..5499
                     /locus_tag="IW781_002621"
     CDS             4177..5499
                     /locus_tag="IW781_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1183594.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5562..5666
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5740..6483
                     /locus_tag="IW781_002622"
     CDS             5740..6483
                     /locus_tag="IW781_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1183595.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6498..7490
                     /locus_tag="IW781_002623"
     CDS             6498..7490
                     /locus_tag="IW781_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1183596.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7502..8239
                     /locus_tag="IW781_002624"
     CDS             7502..8239
                     /locus_tag="IW781_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1183597.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8229..8951
                     /locus_tag="IW781_002625"
     CDS             8229..8951
                     /locus_tag="IW781_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1183598.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9035..>9191)
                     /locus_tag="IW781_002626"
     CDS             complement(9035..>9191)
                     /locus_tag="IW781_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1183599.1"
                     /translation="GRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAF
                     WEANGWY"
BASE COUNT         3068 a         1568 c         1910 g         2645 t
ORIGIN      
        1 agtttaagcg aattttttct cccaataagt aagaaaataa tggcaaaata aaaaaacaaa
       61 ctattttcgc agatcgtttg caaaaatagt ttattttttt ataaagaaga ttttactgaa
      121 taatatcatt ttagtttaca taatgatatt tctgtaaatt aaataaagta gtgaaaaata
      181 agaatcagtg ctctaactgt tcaagaggat caagtggttc aaatgtttct gtagtctgtt
      241 ttgcttccaa ttggggcaaa acagatccaa tttccagata cttcacttta ttcaatgtat
      301 aagatagaat agaacagcta agtaatgtat acagaaaatg tgtgagatcc aaataaagca
      361 aggagacaac taaaatacca aaagtaatca agaaaagact tttgccaatc ttaatatgaa
      421 aatatttttc aatggcttga taaatgatat ctgttccgcc tgtgcttccg ccgaatcgaa
      481 agacgagacc gcaaccaatg ccagaaagaa tacctgtcat caaactattg atcagtagac
      541 tatctacagc aaaatgtata tgaaggctct cccataaatg taaaaagaaa actagcgcaa
      601 atgttccgac gatcgtccgg agaaaagcgt gttttccaaa aagaaacaat gagataagca
      661 ataaaggtaa attaaaaagc agtgaagtga cagaaggagc gaatccaaaa agattatgca
      721 aaagcaaagc aataccagta accccaccat ctgccagctt agatggaata ttgattcctg
      781 tcacagctaa tccgtagatt cctgcgccta aaatgattcc gcacaattct gtaaataaat
      841 ttaatttttt catttctagc ctcttttctt ttgaaaattt tcagcaactt ctttattata
      901 ctgacttata ctcttacttc aagaggaaaa tgaaaaaaaa ttatttttgg ctgtttcttt
      961 ttatttatca aataaggagt ggaaaaatgc gagcagcaga cttattgaca attcttacaa
     1021 aatcacccaa aaaaacagag tatcaaggag tttttttggc tcaagaacaa aaactggtgc
     1081 ctgtgacaat ggtccagata aataaagcaa atcaatttat ctttttattt gaacacggca
     1141 aagcaccact tgctatgaaa gacatctttc tttttttgat gaagaaccgt gagaaagctg
     1201 tttattatca gtcaaaagaa gaactcattc ctctttatgg agtgaaagag atagaaaata
     1261 aagtggtgat ataagtaaaa ataagaaaac aagacgttta tgatgtcaaa acaaccattt
     1321 tttacatttt tggcaaaaaa gtgtcatgat acagtataat attcgttgaa caggccaata
     1381 aaggtgttct cccccacgag atttctactt tattggctgt ttctttttat taaaataaaa
     1441 gttgtgtgtg taaaaaagat atagctgatt ccaagtgaca atttcgtata gatttttatg
     1501 gtattcgtag tgaaaaaacg agaaccaaaa agttgatccg actttgttgc ttggattctt
     1561 tttttgtcag catatttaga ggtatttttt tctttttttt gttcgtgctg taaggttaag
     1621 gcaagacaag ttagttggga aaaaggaaag aaatgtttat acttaaagaa taacagaaag
     1681 gaaggtcgca tgaataattt tatacaaaca ttaagtgaaa gaaaaggcga gttgctggaa
     1741 gctgcatttc aacatctgtc catttctctc atttctttgc tgattgcagc actgattgct
     1801 attcctttag cgatttgggc agccaatcat aaaaaaatgg cagaagtctt attgcagatc
     1861 acgggtgtgc tccaaacgat tccttcactt gccttattag gtttacttat cccatttgta
     1921 gggatcggaa ctgttccagc attgattgca cttgttattt acgctttgct tccgattttc
     1981 caaaacacgt atattggtct agctgaaatt gacccgtcta ttgaagaagc agcggtagca
     2041 tttggtatgt ccagaatgag acgcttgctg aaagtagagc ttcccatcgc attacctgtc
     2101 attatctcag gtatccgaac tgccttagtt ttaatcatcg gtacggctac tttggcagca
     2161 ttgatcggag caggtggatt agggacattt attttgttag gtattgaccg aaatacacct
     2221 gttttgacat tgattggtgc gatcagttct gccttgcttg caattatatt cagcggactg
     2281 attcgctggt tgcagcataa gaaacctcga tattccatca ttactttagc agcagtggtg
     2341 tgtttatttg gcggcgtttc tttatatcag tctgccttgt ttaaaaatga aaccatcacg
     2401 attgctggaa aattaggagc ggaacctgaa atcctgatcc aaatgtataa agaattgatt
     2461 gaagacaaca caaaaacaga agttgaatta aaaccgaatt ttggcaagac gagttttttg
     2521 ttcaatgcac tggatcaagg tcaaatcgat atctatcctg agttcacagg tactgtccta
     2581 gaaagcttgg tgaaggtacc tgaagcaatg aaaagtgctg atttgacaaa agaaaaaaca
     2641 tatgacgaag cagaagaatt attgaataag caattcgata tgacattatt aaaaccaatg
     2701 gcttaccaaa atacgtatgc cttggcagtc aagagatctt ttgcgaacga acatcaactt
     2761 aaaaagattt ccgatttggc agctatctct gatcagatca aagcgggatt cacactggaa
     2821 tttattgatc gttcagatgg atataaagga atccaagaag catatggctt gaatttttca
     2881 agtgtacaaa gcatggagcc aagtttgcgt taccaagcaa tcaataatgg agatgtcaat
     2941 attgtggatg cctattccac agatagccag ctcaaggagt acgatctagt gactctagaa
     3001 gatgatcgag ggttattccc agcttatcaa ggcgcaccgc tgatgaaaaa agattttgct
     3061 aaagaacatc ctgaaatcgt taaagcactg aataaattaa gcggaaaaat aacagaggat
     3121 caaatgattg atatgaatta ccaagtcaat gtcgaaaaga aaaagccgac agaagtagca
     3181 cgaatcttcc tgaaaaatga aggattagta aaggaggaag atcagtgact aaattaatcg
     3241 agttcaaaca tgtgcaaaaa aagtatgatg gaaagtatgt catcgatgac ctaaatctgg
     3301 caatcaaaaa aggagaaatc tttgttttgg ttggtccttc cggtagtgga aaaacaacta
     3361 ctttgaaaat gatcaatgga ttatctaaac catcagcagg agacatctac ttcaaaggaa
     3421 aatcgttaaa tgaatataat ctgcagaaaa tgcggtggaa tatgggatat gtgctgcagc
     3481 aaatcgcttt gttcccgacc atgacagtca agcaaaatat tgaagtcatc cctgaaatgt
     3541 taggttggga aaagcagaaa agagcagacc gtgtagatga acttcttcaa aaagttggct
     3601 tatctcctga tatttatcga gatcgtatgc ctagagagtt gtctggagga gaacagcaac
     3661 ggatcgggat catccgagct attgctgcaa gcccagatgt gattttgatg gacgagccat
     3721 ttagcgcatt agatccgatt tcaagaaact cattacaaga attggttttg tccttacacg
     3781 aagaattggg cacgaccatc gttttcgtta cgcacaatat ggaagaagca atcaagctag
     3841 gtgatcgtat tgcatttatg aaggatggag aaattatcca atgtgacaca ccagagcagt
     3901 tattgatgaa tcctaaaaac gactacgttc ggcatttctt tgatgaaccg aaacagacga
     3961 aagagtggcg agtagaagat ttagtcgtca atggatattt cttaaatgaa ataccagaaa
     4021 atgctcgtca acaagtttgc tttgatacgc ctatgaaaga agtatattcg cttctttcgg
     4081 tgtatccaag cattgtgata gtagaaaagc aaagaagtat tgggtcgctg acaagtaaag
     4141 aaatctttgc ttttttaagt cgagaggagg aaggaaatga aaacatatga tgcaatcatc
     4201 atcggttcag gtgtgagcgg cttaagtgct gcttatggat tgaaagaagc aggtaaaaca
     4261 gtactagtgg tcgaagaaga tctatggggc gggacttgtc ctaaccgtgg atgtgatcct
     4321 aaaaaagtct tgttaagtgc agtagaggca cgtaatcggg taaaacaatt gagtggaaaa
     4381 ggttttaacg agatcccaac tgccaactgg gaagaacttc aaaaattcaa acgaacattt
     4441 acggatccag tcccagaaag ccgaaaaaaa caattagcag aagctgaaat cgatcatctt
     4501 tcaggaaccg ctcgtttttt ggatgattca tctattgaag tcaacgaaga agtcttccat
     4561 gcggattact tagttttggc aaccgggcaa agacctacga tcttaccagt ggagggaaag
     4621 gaatacttga aaactagtgc agacttttta tcattgcctg tactgcctaa agaaatcatt
     4681 tttatcggtg gtggttatat tgcttttgag ctagctacga ttgccaatgc tgcagggagc
     4741 aaagtaacca ttgtccatca taatcaacgg ccattaaaag aatttgaggc gtctttggta
     4801 gaggaagccg tccatcaaat ggaagcatct ggcattcagt ttgcttttgg tgttgaaacc
     4861 caaaagatca tttcagaagg tactcgctat cgtttagtcg gaaaagagac ggaattagta
     4921 gcagatatga ttttttgtgc gactgggaga cagccgaata cagaatcttt agcacttgag
     4981 caagcgaata tcgtctttga taaacacgga attgcagtca atgattatct gcaaacgagc
     5041 aatccaaaaa tttttgcgtg tggagatatt gtttcaagga aaaccccaaa attgacacca
     5101 gtcgccactt ttgaaggaaa ttatgtagct aagcggataa cggatgcaac aagcgaacct
     5161 atcaaatatc cgattatacc tacgatcgtc tacgccagtc ctaaacttgc tgaagtagga
     5221 gttacaaaaa gtcacgcctc ttcgtcagat caagtggtag agatggatct taccagttgg
     5281 ttcacctatc atcgtgtcaa tgaaccagtt gctaaggctg aattgacctt tgaccagcaa
     5341 aattatttga ttggtgctgc agtgattagc gagcaagcag acgaattgat cgatgatttg
     5401 acactcgtca tcaatcaaaa attgactaaa aaagagttgg atagctatat catggggtat
     5461 ccgacattgg caagtgatct ctcctattta ttgaaataat cagcatgata actaaagcgc
     5521 ttgttgcttt ttccttcttg tgctacactt taaaaaatga atacctgttt ctggggatgt
     5581 cgtagacgac atgagagtaa gttgtgaact tagacccatt gaacctgtca gttaatactg
     5641 gtgaagggag aaacatagca gacgcttgaa gcgggttact atgtcgtgcc catttcaagc
     5701 gttttttgtt attcatatta gaagaaagga ggtaacaaaa tgaagaaaaa tctttatcca
     5761 gcagtcagta tggcattcat tttaattatt tggcagattg ttgtcatagt ttggaaaata
     5821 cctgccttta ttttacccgg acctgctgct gtattttctg cattagcggc agatgcacaa
     5881 gtattacttt cccattctgt cacgacatta aatgaagcag tgcttggttt ggtaatagct
     5941 agcgttcttt cttttattac tgccttggca atggattatt ggaagttttt agaaatgtct
     6001 ttttatccgt tactagtgat ttcacaaatg ttgccaatca tggtgttagg gccattactt
     6061 accctatggt ttggtttcgg tatgcttcca aaagtgatcc ttgttgtctt gataagctat
     6121 tttccgatcg tagtttcttt ttcggataca ttgaggaaag tatcaaaaga gcaaatcatc
     6181 tttttgaaaa cgatgggggc agatacaggg aagatttatc gaatatataa aataccagcg
     6241 ggtttatccg gcttcttttc cggtttaaaa gtggctgcta cttattgtgt cagcggagca
     6301 gtcgttggag aatggctaag cgctcaagca ggcttagggt attacatgat acgcgtaaaa
     6361 aacagctacc agatcgataa agtttttgct gcgatcattt gtgtcattct attgagttta
     6421 ttgctgaatg gcgcttgttc attattgaaa aaagggtatt atcgtatgct ttatattttt
     6481 taagagaagg ggatcaaatg aaaaaattat ggcttttatt accgctactg ttgcttttat
     6541 ccgcttgtgg cactgcaaaa gaaacaagta gcaaacaaga aataaaggat ttgaaaaaag
     6601 tgacacttgt tttggattat gtaccgaata ctaatcatac tggcatctac ctagcaaaag
     6661 aaaaaggcta ttataaagag gctggtttaa atgtccaaat catcgaacca ggagataatt
     6721 cgacaagtat tggtctagta ggtgcggaca aagcacaatt tggtgtaagt tatcaagaag
     6781 atgtcactta tgctcacgca gatggacaga atataccagt caaagcaatt gctacagtga
     6841 tcaaacataa tacttctgga ttcgccaccc tatcagacag taacatccat tcaccaaaag
     6901 attttgaagg aaaaacatat gcaggttggc aaagtccaag tgaagaagct gtcttaaaag
     6961 ctgtgatgga aaaagacggc ggggactttt caaaactgac aatggtagga agcaatggag
     7021 aagggccaga aagcttagga aaatcctcag atatccaatg gtactttgaa ggttgggata
     7081 tgatcaaagc aaaagaagcg ggtattgaac taaactatat cccattgaaa gaattagatg
     7141 aacggttaga ttactatact ccggtaatta ttacgaatga tcaactgatc aaaagtgatc
     7201 cagaattggt acagtctttt atggatgcca cgaaaaaagg ctatcaggaa gctattaagg
     7261 atccaaatga cagtgctaaa ctgttgcaaa aatatgcaaa agaaaatgat cggacgtttt
     7321 tagaagaatc acaagcattc ttgtcaaaaa attatacgga tgatcctgag aattggggat
     7381 tgatggaaga aaaagtttgg cgcaattata cagcttttat gcaggaaaac ggattgatca
     7441 agcaagatgt accttcgcaa gaactattca ctaatcagtt tataaaatag gagtgataga
     7501 tatgtcaatc caaatcagcc acttgaatgt agtcattgaa aacctgaaaa tattagaaga
     7561 catccatttc actatcccta aagatacctt tacatcgatt gtcgcgccaa gcggagccgg
     7621 taagtcaaca ttgctgaaaa ctttaacagg agtccagcct atgacttctg gaacgatcaa
     7681 agtagacgat caaaaagtaa ctggtctcca tacagcattt agttatatgc cgcaagaaga
     7741 tatgttactt ccttggctga gcgtctatca aaacgttacg ttatatcaaa aaatcaatca
     7801 tctaacaatc aatgaagaac aagtaaggga ttatctggat atctttgggt taaacggcta
     7861 tgaacatttt ttgccggaac aattatctgg cgggatgaag cagcgaacgg ccttgttacg
     7921 aacaatcatg aatccttctt cttacttgct tcttgatgaa ccatttggag cgttagatgc
     7981 tatgaccaga gggcagatgc aagactggat gttgaagctg cctaaaaaag caaagcggac
     8041 tactttactt gtgacgcatg atatcgaaga agcaatttat ttatcagaca ggattcttgt
     8101 gctttccgcc cgtccggcac atgtgattgc tgagattcaa gtgcctgaaa aacgacggtc
     8161 acgtgaatgg ctactgcagc aatcagagtt gaagcaaacg atttatcagt tattggcagg
     8221 tgaattagat gttaagtgat gctcattgtc atttagatgg gagtcagagt ttagcactcc
     8281 ttcagcagga acacacgatt ttgacgatca tcaattgtga ttcgccagaa gaatggaagg
     8341 aaaacagaca gcttgcagca agtaagactc aagccttaag ttacggtatc catccttgga
     8401 aagcagatag ctatacgttt gaacaagttg agcctttttt gaaaaaagca aggatcatcg
     8461 gtgaaatagg gttagataat atttggacga atgtaccgat gactacacaa aaaaaagtat
     8521 ttgagcgaca gcttgctttt gcagccataa atgagaaacc tgttgtactg cacacaaaag
     8581 gatgcgaaaa agagatctta aattatatcc aagcttaccc taatcactat ttgatccatt
     8641 ggtattcttc tttggactat caacaagact atattgattt aggctgttat ttcagcattg
     8701 gtcttgattt gaaaaagaat ccagcagtct ggcaactagc taaagcagtc ccaatcgacc
     8761 acttacttat tgaaacggat ggactacatg cagcagagtg ggctttaggg gggatttttt
     8821 cagaaaaaga ttatgtctca ctgcttgaag aacagttaaa gacaatcgct gagatcaaag
     8881 agctatcagt tgaagaagtt aaaaaaaata cttatcaaaa cttggaacgt ttcattagat
     8941 taggtaaata aaaaagagat gtgacacaac tagtgtcgca cctctttttg gctttctttg
     9001 tataaaagta aactgtttga ttaataaatg aatcttagta ccaaccgttt gcttcccaga
     9061 atgctttcgc cgcttcccaa gaaccataac gtgaagtaac atattgttct gctactcttt
     9121 cttggtttgc agcagagtag tcaccattta agtaagaaga atctaattgg taacgtccga
     9181 tgtaacgtcc g
//
LOCUS       DACEOM010000099         9029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEOM010000099 DACEOM010000000
VERSION     DACEOM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.103"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            61..594
                     /locus_tag="IW781_002627"
     CDS             61..594
                     /locus_tag="IW781_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183600.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(720..1397)
                     /gene="nth"
                     /locus_tag="IW781_002628"
     CDS             complement(720..1397)
                     /gene="nth"
                     /locus_tag="IW781_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1183601.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1404..2102)
                     /locus_tag="IW781_002629"
     CDS             complement(1404..2102)
                     /locus_tag="IW781_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1183602.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2370..3554
                     /locus_tag="IW781_002630"
     CDS             2370..3554
                     /locus_tag="IW781_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1183603.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3560..4003)
                     /locus_tag="IW781_002631"
     CDS             complement(3560..4003)
                     /locus_tag="IW781_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1183604.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4229..4516)
                     /locus_tag="IW781_002632"
     CDS             complement(4229..4516)
                     /locus_tag="IW781_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183605.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4687..6039)
                     /locus_tag="IW781_002633"
     CDS             complement(4687..6039)
                     /locus_tag="IW781_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1183606.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6312..6758)
                     /locus_tag="IW781_002634"
     CDS             complement(6312..6758)
                     /locus_tag="IW781_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1183607.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6994..7920)
                     /gene="cysK"
                     /locus_tag="IW781_002635"
     CDS             complement(6994..7920)
                     /gene="cysK"
                     /locus_tag="IW781_002635"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1183608.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(8060..8947)
                     /locus_tag="IW781_002636"
     CDS             complement(8060..8947)
                     /locus_tag="IW781_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1183609.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2477 a         1824 c         1474 g         3254 t
ORIGIN      
        1 ttttatttag tgccacttct taataatccc attgttatta tcaaataaaa aatacaatat
       61 atggttttga ataatttttt gaaagaaggc ttcattttga gttatctgat agtatgctta
      121 attatcataa tttgtctgca ccatcttttt ttatcgaaaa caatacctcg ttattccaag
      181 aataaaaaag ctgaaaaatt ttgtttatta ttaaataaat ttactttaat tgctcctatc
      241 cttgcattta ttattttttc tgtactttta agcactactc ttaaaggaaa atttatggaa
      301 agaagctctc atgcaatgat tctcactttt ttatggttgt tgttcaccag aatctatatt
      361 tttctgatga gcctgaaacc gcccaaatcc attagtttat gtttagttat aaatggtatt
      421 tttttattat cacttatcat ttttattaca cctttagata gatacgtcac ttatttatat
      481 aatcctttgg aatactggac ttattttata ggtatattgg aaggcattat tttctacatc
      541 gggtattttc ccaataaaaa taacaacttt tatttttatc gcaataaatt gtaataagtt
      601 accatcctag ctactgagta tgtacattta ctttttcatg caaaaaagat aaacgaaaca
      661 gcttttctcc ataataacaa gataattcat acatttattt gcaattaaag aaaccacgtc
      721 tacaggcgtg gtttcttttt tagcgccttt tcttttagac gcatccgatt tttcccatct
      781 tgacatattg atagcaaagg acatacctcg cacttgggat ttcttgccgt gcaatgatat
      841 ctaccaaaaa aaatcagtgt atgatgtgtc tttacccata attcttgtgg tactttccgc
      901 atcagtgttt cttctacttc catgaccgta gcatctagtt tgcagatcct caggcgtttt
      961 gagacacgtt ccacgtgggt atctactgcg attgctggta tcccgaatgc atcccccaat
     1021 acgacatttg ctgtctttct gcctacacca ggaagagaca tcaattcttc tcttgacgtt
     1081 ggcacttgcc catcgaaacg ttcaatcagc tgctgtgcac acgctttgat gttttttgct
     1141 ttatttcgat acaaaccaat cgtttttatt ttaagaatga tttcatcgat tgaagcttct
     1201 gctaacgcat cgggtgtcgg aaaagaggca aacaaatctg gcgttgcttt attcactgat
     1261 acatctgtcg cctgggcact taagatcact gcaatcaata actcaaatgg attattgtgc
     1321 ttgagttctc catgtgcttc gggaaacatt ccatacatcg tttctagagc ttccattgtt
     1381 ttttgtttgc ttaacatatt ccgctactcc tccaaccaat ttttcagtga gactttcggc
     1441 aattcttctt gttgagctga ctgttctact tctctttgaa gaagctgctg ttttcttcgt
     1501 ttttggtctt cttccacttg ttgttttgta cgtagatttt ttctttccca cgatagcaaa
     1561 atgcggtcaa cgtaattaaa actataagcc tgatttaata cggcttcccg cagtgctagc
     1621 ttcaaaattt ccggctgata atggtcttct tctagccatt gtccgatccg ctggaactcg
     1681 atcgaagaaa gcgggcgtcc aaattcttgt tcaaataatt gatacactga ctggatttct
     1741 ttttcttgtg ttttttcttc gtattttttt tcttgtgttt ttaaatactc acctaataga
     1801 tcatagatgg ggtaaagatt atatcgatct gcttttttac cgttgacaat cgtcgtttct
     1861 atcttgataa atccatttgt tacgagacga tccagtattt gaaaaatctg atcttgtttg
     1921 attcccatat ccatagcaat catctgcaaa tcagggaaag tatctccttc aagctgtgcc
     1981 atatgcaact gtagaacaaa taaaaattct tcagattgta gaccgatcct atgataattt
     2041 ttaagtaaaa gattagatat gactgtttgt ccagcgctta agtaattttc taaattcaac
     2101 atgatttctc ctccgctcaa tcacagtata cgtaaaaaaa acatgggaag caagaacagg
     2161 tttcttttct atttacttca ctgatcggtc agatttacaa tgtcgtctac ttgatttttg
     2221 tttgttcatg tttttgatgc gtatcttcac ttttcggaat aaataaatta agtagatttc
     2281 gctgttcctt agaatgttga caaggaaaca aaattactat acactatttt tgtttccaag
     2341 ttaacattat tgcataaagg agtttttaca tgtacagttt cacaaataaa cattctaaag
     2401 tttttggata cgtctctgtc ttattcactg gattaggatt tactatcgtc agtcctgtac
     2461 ttccgttttt aacgctgcct tattctcact ccatacatca gcaagctttc tatatcactt
     2521 tgctcatgtc tgtttatgct ctagctgcct tcttatctgc ccccattctc ggtagcttaa
     2581 gtgatcattt tgggcgtcat ccgatcctta ttatcagttt attaggatca agtatcggat
     2641 atttgctttt tggtttagga aattcgatat ggatgttgtt ccttggtcgt atcattgaag
     2701 gattgacggc aggagaaatc tccaccttat atgcttactt tgccgacatc acggaaccta
     2761 atgaaagaac caaagttttt ggatggatgg gtgctttagt cggtatagga acaacgctgg
     2821 gtcctattat agggggtcta ttagctgagt tggggaattc tgttcctata tttattggag
     2881 cactctttac tttcttaaat gccgtttatg gctacacatt tatgctagaa agccttccaa
     2941 taaaaaaacg ttcagtagat ttaagtttct cccatgtaag accttttcac caattacaac
     3001 aattattcaa gatttcatcc gttattcctt tattgacagc aggttttgct gtttggttgg
     3061 cagctggctc tcttcaatct atcttttctc aattttcgat tgacgccttt cagtggaagg
     3121 ctgggttggt tggtttaagc ttctctttga ttggaatctt ggatactgtc tcgcaattgt
     3181 tcattatgcc tcgattgctc aaaaaatttt cagaacagca gatcacaaga atagggatgt
     3241 tcagtgaaat cctcgcctat ctattcatca cattatctgg aattcttcta ctgccgattt
     3301 tgtttttatt tgggattatc tgttatggtt tcggtgattc tatttttact cctgtattca
     3361 atggccaact atccaattct gtctcagata accagcaagg tcttgtaatg ggcggtaccc
     3421 aaagtataca atctctatcg cgcgtcatcg gcccgctaat cgcaggacag ctttatgctg
     3481 cagcaccatt tcttccgacg gtattcggat ttttgcttat gatttttgcc tattatcact
     3541 acaataaaaa atagtagctt catttgctac ttcgtataaa ttctctgtat cgaccgataa
     3601 aacgaaatac tgtgtcaaat tcttcttttg acatattttc aaatacagta gcatctcgct
     3661 gtacccattt ttgatggagt tgctcgtgtc tattccttat tttttgtccg attggcgtca
     3721 attgaaaata gatttctttt ttattctctt cgctttgata agtgtttatg atccctctag
     3781 ctatcagttt ttttgttagc ttactgatgg ctcctctagt catgtaacta gcattcgcta
     3841 attttgtcac gttaggatca gggattttcc cgatatgttc aatcacttct agttcattta
     3901 gagtaaatcc tgcaaattct ttttccattt ctggtttatt acgatgaata atctcattag
     3961 ctaaatccag atattcttct attagcagtt gtcttttatc catttctaca ccttcttttt
     4021 ctttcattat attgtttttt tgtttcctat tcaacaaaga taaaaatatt ttgtaggaaa
     4081 acccaatctg aaaccaagaa aaaaaggggt tgacaaagct tgtcataacc cctttttctg
     4141 attaaaagat agtaaagaga cagtcatcgg taattgtgat gattttttgt catcacttaa
     4201 ggatgagcgg ccttttcaaa aactgtcttt aaagatcatc atcagtttct agcacatatg
     4261 gctgaccttc atatgtgtac tctgtattca atgtgccgtc attttcatct gagtagatgc
     4321 gtctactgaa aggaaaacga tgagaaacat tatctttggc gtcatttgct ggcatcgtgt
     4381 aatattcctc ttgcgtttca ttcaactgag agagtacttg atctgccaac tcatcaatat
     4441 tgatcgttaa tttcttcgaa gcacttttat aatcttctaa aaatgcatct tgttggacaa
     4501 aatattcttt attcatcttc tttctcacct ttcttttcaa aatcaaataa agctgcttgt
     4561 tcttttatgt aacagcttta tctttgctgc ttattgtgac aaaaaattcg aagaaagaca
     4621 actaattgta cttttcacaa aaaaagacag agggtcgtga caaaattgtg tcacaaccct
     4681 ttttctttat tgtgcagttt cgatttcgcc tagcgctgct tgtgcaagta ggttcaagta
     4741 attccatggg cggtcaaaat gtggttggaa gaagaaatct gaaagtgcga gatcttctac
     4801 agtcatttta ttttgaacag ctaaagacat ggtatttgct gattgtgtaa tgtcgtattt
     4861 ggacatgaac tgagcaccta cgatacgatt cgttcctttt tcatagacta gctccatcat
     4921 tactttttca gtagttggca tgaactctgg acgataatta tcttcaaata cagtcgcttg
     4981 gacttctaga tcgttcaaag ttgcgctttc tttggtcaca cctgtcgaac cgatcgtcca
     5041 gccaaatagg taaagtcctg aagttccttg agtaccacga tacgccattt tatgatctgt
     5101 caagttattt cccacaagca aaccttgacg aacggcattt gttgccaaag gaatatagtt
     5161 ttgcgtgcca cttggattgt agttgactac tgcagagtct ccagctgcaa aaatgtctgg
     5221 attacttgtc tgcatgtatt cattcacttt gattgccccg tttggcagtg tatctacttt
     5281 tccttcgatc agttttgtat ttggacggaa gcccacacat aaaatgacca tatctgtatc
     5341 aaatgtatct gttggtgtag ctacttgttt cacttttcct tcttcatctg tgataaattc
     5401 agtaacattt tcgcctagcg caagagtaac accgcggtct gtcaattctt tttctaagat
     5461 atctgtgaat ggtttatcta gatatttatt caagatgcga tctaatccat caatcaatgt
     5521 cacttgtttt ccagattcga caaaagcttc tactaattca ataccgatat atccaccacc
     5581 aacaattact acacgttttg cttctttcgc ttgtgcaata atttcatttg cttgattgta
     5641 gtttttgcat aggagtacat ttttcgcatc gattccacta atcggtggga taataggcca
     5701 tgaaccagtt gtcatcacta atttatcata agaaaccgtt tctgtttctc ctgttttcag
     5761 atcttttgcc gttactgttt tattttctgt atcgacgtct gttacatcgt gttccatttt
     5821 aactgttgct cctaatgaag ataattcttc aggatttgag tagaacaagc ctgctgggtc
     5881 ctttactact ccgccgacat aaagtgcgat accgcaagat aagaaagaaa tattgtcatt
     5941 tctttcatat actgtcactt ctgcttctgg atgatttttt aaaatacttt ttacagctgc
     6001 agtacctgca tgagtacagc caattactac tactttcatt tatggtccct ccgattaaca
     6061 ttttgtcctt cgacaaaact actataacag aaataaaacg gatacaacag tttaatgctt
     6121 gtgaattcac aaaggtttgt ttgtagtttc attccttttc aagcataaga acccaatatc
     6181 tacaagattc ttattttaat aatattcact tttatatcta agattgtgaa taaaagacaa
     6241 aaaaataaaa aagctagcgt tttttttaca cagctagctt tttttaggta taacacttgt
     6301 agaaactgtt tttacatttg cgcctttttt gctgactctt tacaatccgg acagagtcca
     6361 tacaattcca accgatgttc attaatctca aaatcagtca gtctgcttgc agccatttct
     6421 atatcttcaa gccctgggta atgaaaatca acaatcttac cgcagttttc acaaattaca
     6481 tgataatgac gctttgaact gaaatcaaat cggctagaag catcgccgta cttcatttct
     6541 tgaacaaatc caatcgaagt aaataagcga aggttattgt aaacagtcgc aacactcata
     6601 tttggaaacc gatgttcaag tgctttataa atctcatcag ccgttggatg ggaatgatgt
     6661 tcaatcaaat attctaaaac cgcgtaccgt tgaggtgtga ttcgaatgtt ctcttttttt
     6721 aacttttcga taacctcttc gactaactta ttttccatgt aaacacccca cgttgttatt
     6781 ctcgtcttat catactattt ttacatttta aaatccactt aacatgaaaa gaaaattcaa
     6841 ttttattgta ttttaatgaa aaaagacgta aaacagtcgc gttcactatt ctacgtctct
     6901 attcaaataa tttttctcat cataaatttt tgtgtgttct tttcatcttt aaaagttttt
     6961 tacataaacc tatctggaga aaaaccaagt gccttatatc tcttggtaaa gggctgtaga
     7021 taaataacgt tcaccattgt cgggaaccac agctaatacc ttcttgcctt ttcctaattc
     7081 ttttgcgact tttagcgcgg cagcaattgc agcgccagat gagatgccta ctaaaatccc
     7141 ttcttttcgc ccaacttctc tagctgtttc catcgcttcg tctccactaa tagaaaggac
     7201 tttatcatac acagaagtat ccagtgtttt aggaacaaaa cctgtaccga ttccttggat
     7261 tttgtgaggt cccgcttctt tcccttctaa aatagcagat tctgctggtt ctaccccgat
     7321 taattcgatt ttcggataca cacgttttaa ttctcggcca gcgccagtga tcgttcctcc
     7381 tgtaccgata cctgcaacaa acgcgtccaa gccatttact ccaaatgctt gatgtatctc
     7441 aggaccagtc gtcttctcat gcacaagagg atttgcttca ttttcaaatt gtaatggtaa
     7501 aaagtagccg ttttcttttg ccaaacgttc tgcttctctg attgatccag aaataccatc
     7561 cgcaccagga gtcaaaatca actctgcgcc gtaccctttc atcaataaac gtctttcgat
     7621 actcatcgtc tcaggcatca cgatgattac tttatatcct tttgcaactc ctaccatcga
     7681 caatccgatt cctgtatttc cagaagtagg ttcaatgata gtatcacctg gtttcagtag
     7741 accatcgtgt tctgcttttt ctatcatact taaagcaata cgatctttta cacttcctcc
     7801 tggattaaaa aactcaagct ttacaaaaac atctgctgaa tcttctggta cgattttatt
     7861 taatttgacg attggcgttt tgccaatcaa ttcggtgact gagttataga tagctgtcat
     7921 cttcaattcc tccttgtctt tgctcttctt acttatcata atacaatctg ttcatttatg
     7981 gcgagtgatt cgtctttgac tagcaaaaaa aagacaaaga tataccatat atggtaattt
     8041 ctttgccttt ttatctaaat caaacgttta gttcttgacc aactaataaa gaataactgg
     8101 acatagacat gccatttttc gccattagat catcaacaga catgccaaat ttttgtgcaa
     8161 tatcccatag cgtatcacct gacacgactg tatatgtctg accattattt gtatttacat
     8221 ctgttgttga agatgatgac gttgaagtcg tcgtagtagt cgttgttgtt gtacttgtgg
     8281 ttgttgtagc cgaacctgtt gatggcgtat caaaacgggt taaattataa gtattaatca
     8341 atgagttcaa tttggcccca taatttggat cggtagcata acgacctgtc aagtacgctg
     8401 ttgcatcata aaaacttgtt gtattgcttt tccacacgcc agcataatga taatcgcccg
     8461 tggaaaaact ggttgaacga agaacattcg catggtcttg gaaggattcc caataagaag
     8521 aatattttcg gaatggctca ttcatcgtta cccattgtcc gtttacatat tctttggttg
     8581 ccatataaac gacctgtccg ttatagcttc ctttcacacc gaatagatta taatttggcg
     8641 cactagctaa tccggaagtg ccatatgcac tttccaacaa cgcttgagcg atcatgactg
     8701 aagcatacaa gtcattactt gcagcaactt ctgacgcaga ccagccgatt tgatcgataa
     8761 acgcttgagg atcagcgact gtttgagatc cttgttcatc tgcatgaaca tgttgcggta
     8821 ccgctgtaac catcatcggc ccaactaaaa gactcgttcc aattaaagga gcaatcgctt
     8881 ttttattgat caccattttt cttttaggtc tatggtgccg ggcactgcgg gtcttcagct
     8941 cttgcattca aattcctcct caaaataaaa aaacttaata aaaagttctc ttcttaaaac
     9001 aaacttaatt aattaaatta tacttttaa
//
LOCUS       DACEOM010000100         8722 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEOM010000100 DACEOM010000000
VERSION     DACEOM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.104"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..2199
                     /locus_tag="IW781_002637"
     CDS             7..2199
                     /locus_tag="IW781_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1183610.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            2216..2692
                     /gene="ybeY"
                     /locus_tag="IW781_002638"
     CDS             2216..2692
                     /gene="ybeY"
                     /locus_tag="IW781_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1183611.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            2670..3071
                     /locus_tag="IW781_002639"
     CDS             2670..3071
                     /locus_tag="IW781_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1183612.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            3086..3985
                     /locus_tag="IW781_002640"
     CDS             3086..3985
                     /locus_tag="IW781_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1183613.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            4128..4940
                     /gene="recO"
                     /locus_tag="IW781_002641"
     CDS             4128..4940
                     /gene="recO"
                     /locus_tag="IW781_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1183614.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            5324..6241
                     /gene="glyQ"
                     /locus_tag="IW781_002642"
     CDS             5324..6241
                     /gene="glyQ"
                     /locus_tag="IW781_002642"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1183615.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            6243..8318
                     /locus_tag="IW781_002643"
     CDS             6243..8318
                     /locus_tag="IW781_002643"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1183616.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         2943 a         1560 c         1853 g         2366 t
ORIGIN      
        1 aagcacatgt tgaataaaac actgcagaaa ctacaagaaa aacttggaaa ggcagcattg
       61 cctttcctgc tgttcttgtt ttttatcatt atctgcggcg ttacatttag tagtgtcaaa
      121 caaaaggcca atgatttcag agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa
      181 acgatcgaga acacggaaga aaccgaacaa aaaagaaaac tggcagcaga agcagtcact
      241 ccagaataca cttaccaaaa agatcttgca gatgaccaga acaatcggat caagcaacta
      301 tttgaattga tcgataaaac gaatgatgcc atcaacaaat cttatgacga aaagattgat
      361 aaggcaaagg aagatgaaaa tatcccaaag cctactacag aagagcggat cgccgcgttg
      421 aagaaaaatt ttgaaaatgt gaatgcagaa aatgttgcgt tttatcaaaa actaccgaac
      481 aatttttatt cgacgatatt tgaaatgact gaaactgaga tccatactgt gagggatgag
      541 agtctgaagc tcattgacga acagatgagc aaacaagttc gtgaaagtga attggaagct
      601 tttaaacaag aagcagaaga gcaaatccaa tatttgaatg tcacaccagc acaacaacaa
      661 atgattcgct acttagtgga tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag
      721 acagaagaat tgaaacaatc cgcacgagaa gctgtacaac cagtgatgat cttccaagga
      781 gaaatcattg ttcgtgaagg taatcaaatc gatgcttcag ctatgaaaaa attggaatta
      841 ctaggactga ctaatcaaac aacttcgatt tttccgctaa ttgccatggt tttagctgtt
      901 ttgcttcaaa tcggcgtact gatttataat tcacttcaat tccaaaatgc aggagaaaga
      961 acaaaatacg tgcttttcta tgtgacagct atgtcagtca gcatagtgct gatgaaattt
     1021 ttccaattat tccaaacgga acaagctact tatatcccgc tgttttatcc cgctgctttt
     1081 gcaccacttg ttttaagctt tttcttgaac cgaagagcag ggatcatggc tggtattttc
     1141 caagcagtat ctgcactttt cattttttat gattcgattg gcacgaactt tctaacgatt
     1201 atcttgatgt cttatgtttt ctctgggctc atggcaacga ttgtcagacg caaacggatc
     1261 tcagaacaag gattatctgc tgccatgtgg gtcattattt ttcctgtgat gatggatgtc
     1321 attttgatcg tctatcaagg aatgagcttt ggtgatgcaa cgacttggtt aatgcttgtc
     1381 tgcgggttca ctggtgcact cttctcattc ttgctgacga tcggtctaca cccatatatt
     1441 gaattgatgg tcaatgatga cagtgtaatt gtattaaatg aactcagcaa tccaaatcat
     1501 cctttattga aacagttgct ggaagaagcg ccaggtactt atcatcatag tatgatggta
     1561 gctaatctaa gtgctaacgc tgtagcagaa atcggcggac gttctttgct aactcgagta
     1621 gcttgctatt atcatgatat cggcaagatc aaacatgcta gctttttcgt tgagaacttg
     1681 ccttcaggag cggaaaaccc gcacaatttc ttattgccag aagacagtaa acatattatc
     1741 tttggacatg tgacagatgg agccaaaata ttggaagatt ataatatgcc gcaaatggtg
     1801 atcgatattt gcaaacagca tcatggaacc acgctaatgc gttatttcta catcaaagcg
     1861 aaagaacgca atccggaagt aacagaagag caattccgtt atcctggacc aaaaccgcaa
     1921 agtcgtgaag cagggatcgt caatattgct gacagttgtg aggctgcagt acgggcaatg
     1981 gatcatccga cgagcgaaaa aatcgaaagt tttgtccata atcttatcga agagcgtatt
     2041 tccgatggtc aattagacga tagtggactg accttgaaag aaatccgaaa agtggaaaaa
     2101 tcattgatca gcggtttaag tagcaccttc cattctcgga tcaaatatcc taaaatgaaa
     2161 acagaagttg agaagataaa ggaagaacaa gaagaataga aagaagcgat gtataatgga
     2221 tattaccttt atggatgaaa caaaccaagt ctcagaagag aaaatgaaag aaatcgatga
     2281 cttgctgcaa tttgcagcat cttatttaaa gctgccagag gaaacagaaa tgtctgttac
     2341 tttcatggac aatgcggcaa tccaagtaat caatcgtgat taccgtggaa aagatgcacc
     2401 gacagacgtc atcagttttg ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt
     2461 tgaagatgga gagaatccat tgcctcgtgt actaggagac atcatgattt ctattgaacg
     2521 tgctgctgaa caagcagaag cttacggcca ttcatatgat cgggaacttg gctttttagc
     2581 ggtacatgga ttcttgcata tcaacgggta tgaccatatg acaccagaag atgagaaaga
     2641 aatgtttggt ttacagaaag agatcttgga tgcctatgga ctcaaaagat aaaaacgtag
     2701 agaaaaacaa acatttcctg atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt
     2761 ttgaggaaga acgcaatatg aggaaacatg ttgcgttcgg tatccttgct ttatttatgg
     2821 gcgtgatttt ccagcttgat cggatagagt ggttatggct gcttttagct gtttttctcg
     2881 tttggatagt agagatcatc aatactgttt ttgaaaatgt agtggacatg tttacggatt
     2941 ttcattttca tccgattggg aaaaaaatca aagatatggc agctggagca gttttagtga
     3001 ccgcttgttt tgctgtcatc attgggttga ttttatttgt ccctaaaata tatcatttgt
     3061 tgtttaatta aaaaggagaa cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt
     3121 cggccgcccg aatgtaggaa aatccacatt attgaaccga atcgtgggtc aaaaaattgc
     3181 cattatgagt gacaaggcac agacaactag aaataaaatc caaggagtat atactacacc
     3241 agaagctcag ctgattttta tcgatacacc aggaatccat aaaccaaagc atcgtctagg
     3301 agattttatg gtagaagcag cttacagtgc gttgaaagaa gtggatgcag tcttatttat
     3361 gatcagtgct gatcaaaaac gcggccgggg agacgatttt attattgaac gtctaaaaaa
     3421 cgtgcattct ccagtttatc tgattatcaa taaaatcgat aagatccatc cagacgagtt
     3481 gctgggaatc atcgaagatt attcgacgca gatgcctttt gcacaagtga ttccgatctc
     3541 tgctacagaa ggaaacaatg tcgaacgctt aatggatgta ttagtcagtg aaatgccaga
     3601 gggcccacaa tattttccag atgatcaagt gactgatcat cctgaatact ttatcgtttc
     3661 tgaattgatc cgtgagaaag tcttgttttt aacacgagac gaagtgcctc attctgtcgc
     3721 agtcgttgtt gattcgatga agcgaaacga gaatgataaa atccatatcc aggccacgat
     3781 catcgtcgaa agagatagcc aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa
     3841 gcaagtagga acgaaagcaa gaaaggatat cgaaaatctt ttaggagaca aagtatttct
     3901 agaactttgg gtcaaagtcc aaaaagactg gcgagacaaa aaagtctatc tgcaagactt
     3961 tggctaccga aaagacgaat actgatattc tgaacttctt ataggttaag tatagaaata
     4021 aactggataa aaatccgcac gttgagaaaa acggcggaag atcgaaacat aaataatctg
     4081 gatcggcaga atctgctttt ccagatttat tttttaggag ggagaatatg agccagcttg
     4141 cagaatcgaa aggcttgatt ttatctagcc gtaattacaa agaaaaagat aaattagtca
     4201 aaatctttac ggaatcttct ggcaaaatga tgttttatat caaaggaatc catcggcaaa
     4261 atcagccatt agctccggct ttgctcccct ttaccgaagc agtctatatc ggtaattttc
     4321 gaagtgaagg tttgtcgttt ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc
     4381 aagaagatat ctttttgagc ggttatggta cctatttgct aaatctagta gatgcagcaa
     4441 tcgaagatca tcaatatgac ccgcatttat tccaatttac gcaacaagct ctgcagcgga
     4501 tggatcaagg agatgatgca gaaatcatca cgaatatctt tgaagttcaa atactgcaac
     4561 gcttcggaat cgcgccaatt tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat
     4621 ttgactattc ttccaaatct ggcggtgtga tatgcgagaa acactggcca atggacgaac
     4681 accgttatca tgcagatcct cgcgctgttt atttcgttcg aatgttttcg gctatttctt
     4741 atgataagat cagtggaatc cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca
     4801 ttgaccagtt gtatgatgaa tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc
     4861 aaatgaagtc ttgggcacac gtattaaaga cgccagagaa aaatatgcct gaaaaagaga
     4921 tacctgaaaa agaagaataa atgaaagata caacaaaaaa atcaaaacta tttgacaaat
     4981 acagggactt ccgtctatta ttaaccgtag attaaataga tcgttcactg agaggatcta
     5041 ttatataaac aagaaaactg caaaatgaat acgtacattg accaagatca gtaaaaaaca
     5101 cgtggtttag cgagttcggg agagtggaag ccggacccaa acgatttttg aagaggcact
     5161 tgcgagtacg ataaatgaag ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa
     5221 ttattcgtcc ctatgtctgt aatcagacat agggactttt tgtcgttttc tttcctgtat
     5281 tcttattgac agcaacgctt aaaaattgaa aggggcaaaa cagatgagta aaaaattgac
     5341 cgtccaagat atgattttaa cattacaaaa attttggtca tcaaacggct gcatgctgat
     5401 gcaagcttat gacacagaaa aaggagcagg tacgatgagc ccatatacat tcttacgggc
     5461 aatcggaccg gaaccatgga atgctgctta tgtagagcca tcccgccgac cagctgacgg
     5521 gcgttatgga gaaaacccaa accgtttgta tcagcatcat caatttcaag tcgtaatgaa
     5581 accttcacct gaaaatatcc aagagcttta tttagaaagc ttgcgtcttt taggaattga
     5641 tccattagaa cacgatattc gttttgttga agacaactgg gagaacccat cgatggggtg
     5701 tgccggtgtg ggttgggaag tatggcttga tgggatggag atcacacaat tcacgtactt
     5761 ccagcaagtc ggaggactac cttgtaaacc agttacttct gagatcactt atggactaga
     5821 acgtcttgct tcatatattc aagaagttga aagtgtctat gatctggaat gggctgatgg
     5881 tgtgaaatat ggcgagatct tcaaacaacc agaatatgaa cattccaaat attcttttga
     5941 agtaagtgac caagacttgc tactaagcaa ctttgaccgt tttgaaaaag aagctaaacg
     6001 ctgtatcgat gaaaatcttg ttcatccagc gtatgattat attttgaaat gcagccatac
     6061 attcaattta ttagatgctc gcggtgcagt atccgtaaca gaacgagcag gttatctttc
     6121 ccgtatccgc aacatggctc gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt
     6181 aggattccct ttattgaata aaaacacagc aaatccagaa aacatgaagg aggctgaata
     6241 agatggcaaa agacttatta cttgaaatcg ggctggaaga aatgcctgct catgttgtca
     6301 caccaagcat gaaacaatta gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga
     6361 cttatgatac gatcgaaacg ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca
     6421 ttccagaacg tcaagcagac agtgaagaag aagtcaaagg accagccaaa aaaattgctt
     6481 tggacgcaga tggaaactgg acgaaagcag ctgaaggatt tgtccgcgga caaggtctga
     6541 cgacagatgc tatcactttt agagaattaa aaggcgtgga atatgtctat gtaacgaaat
     6601 acactcacgg caaagaagcg aaagaagtat tgacaggttt agcagatgtc attaaaagcc
     6661 tcactttccc agtaacgatg cattgggcag aatacgattt tgaatatatc cgccctattc
     6721 actggatcgt ggcattgtta gatgacgaag taatcccgtt tgagatctta gatgtcaaaa
     6781 caggcagagc ctctcgagga catcgtttct taggcgaaga tgtgacattt gcgcatgcaa
     6841 aagaatatga agaaaaattg aatgaacaat tcgttattgc ttcaccgcaa aaaagacaag
     6901 aaatgatcgt tacacaagca aatgaattag ccgaaaaaaa tcactgggtt attgatctag
     6961 acgaagattt actagaagaa gtcaacaatt tagtcgaata tccaactgtc ttttctggcg
     7021 acttcgaaga aaaatatctt tctgttcctg aagaagtact agtcacttct atgaaagaac
     7081 atcaacgtta tttcgaggta cggaacgatc aaggtatgct gcttcctcat tttatttctg
     7141 ctcgtaatgg tgatcgggta aaattggaaa atgtggttcg aggcaatcaa aaagtattga
     7201 cggctcgttt agaagatgcg gaattctttt ataatgaaga caagaaagct tcaatcgacc
     7261 aatacgttga aaaactaaaa actgttactt tccatgaaaa aatcggtagt atctatgaga
     7321 aaatgcagcg ggtacaagtc attgcccgta tcattggcga acaagtagga ctatctgagg
     7381 aagaattgat ggatctgcaa cgagctgctg aaatctataa atttgatcta gtaaccaaca
     7441 tggtagggga atttcctgaa ctacaaggga tcatgggcga aaaatatgct ctgctgaaag
     7501 gcgagaaacc tgctgtagca acagcgattc gtgagcatta catgcctata tccagtgaag
     7561 gagagctacc tcagtctaca gtaggttctg ttttagcgat tgcagataaa ttagatagca
     7621 ttttttcttt ctttgcagtt gggatgattc caagcgggtc aaacgatcct tatgccttac
     7681 gtcgccaagc atacggtgtc gttcggatca tcgaatcgaa aaaatgggcc tttcctttgt
     7741 ctgttctgca agaaacgatt agtgatgtta tcagtaaaga tacggatcgt ttcggcattg
     7801 gtttatcaga agggcaacaa caggtgatcg actttatcaa aggccgtctg cgccaattat
     7861 tgacaacgaa gaatattcgt catgatgtca tcgaagctgt tttgaatgca gaacaaaaag
     7921 atctgacaaa agtatttgca gcagcacaat tgttcaagca gcatcttgca gatgaagact
     7981 tcaaaccatc gatggaagct ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg
     8041 aaaaacaatc agaagtagac cctgaactgt tcgaaaatga agcggagaaa gaactgcaca
     8101 aagcagtaga ggcaatgaaa aaaagctttg ctgaccaaac gatcaacgaa aattatgatc
     8161 agcttgtaag ccttcgccca ctgatcgaaa attattttga tcagacgatg gtcatggcag
     8221 aagaaccagc agtacgtaag aaccgattaa cgcaattaag tcaaattgct gccatggctt
     8281 tgtcgctagc aagtttagat caaatcatca caaaataatt tctaatatgt aaataaacca
     8341 gagaataggc acaaaatagg ctatttctct ggtttatttg ttataaatat aaataactat
     8401 acccgtggtg ctagttaatt ttttcctagg tattatacaa gtaatttgag tcggtcaatt
     8461 tcaactgaat tatctaaact aactgatcta cttttagata attcagttga aatttattgg
     8521 taaactgtcg cttttctttt taaatggcaa aaatttttaa taatatatga caatttagaa
     8581 atcaactata agactgatta attcaaaatg atcttagaat cattgtatga acactaaata
     8641 ctatattgtt ttctattaat cgttatataa acgattatga attcaaagct actttttatg
     8701 acgtcaatca gagagtgtaa aa
//
LOCUS       DACEOM010000101         8592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEOM010000101 DACEOM010000000
VERSION     DACEOM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.105"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(51..1517)
                     /locus_tag="IW781_002644"
     CDS             complement(51..1517)
                     /locus_tag="IW781_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1183617.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IW781_002645"
     CDS             1904..2500
                     /locus_tag="IW781_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1183618.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IW781_002646"
     CDS             complement(2558..3040)
                     /locus_tag="IW781_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183619.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IW781_002647"
     CDS             complement(3146..3343)
                     /locus_tag="IW781_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183620.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IW781_002648"
     CDS             3638..4396
                     /locus_tag="IW781_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1183621.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IW781_002649"
     CDS             4456..4884
                     /locus_tag="IW781_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183622.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IW781_002650"
     tRNA            4930..5002
                     /locus_tag="IW781_002650"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IW781_002651"
     CDS             complement(5152..5523)
                     /locus_tag="IW781_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1183623.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IW781_002652"
     CDS             5738..5923
                     /locus_tag="IW781_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183624.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IW781_002653"
     CDS             complement(6006..6194)
                     /locus_tag="IW781_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183625.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IW781_002654"
     CDS             complement(6218..6481)
                     /locus_tag="IW781_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183626.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IW781_002655"
     CDS             6639..6896
                     /locus_tag="IW781_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1183627.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IW781_002656"
     CDS             complement(6967..7158)
                     /locus_tag="IW781_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183628.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IW781_002657"
     CDS             complement(7299..7619)
                     /locus_tag="IW781_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183629.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IW781_002658"
     CDS             complement(7642..8076)
                     /locus_tag="IW781_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1183630.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..>8592
                     /locus_tag="IW781_002659"
     CDS             8411..>8592
                     /locus_tag="IW781_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1183631.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2723 a         1695 c         1362 g         2812 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tc
//
LOCUS       DACEOM010000102         8495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEOM010000102 DACEOM010000000
VERSION     DACEOM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.106"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..1010)
                     /gene="menA"
                     /locus_tag="IW781_002660"
     CDS             complement(57..1010)
                     /gene="menA"
                     /locus_tag="IW781_002660"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1183632.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1023..2126)
                     /locus_tag="IW781_002661"
     CDS             complement(1023..2126)
                     /locus_tag="IW781_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1183633.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESTLKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2216..3151)
                     /locus_tag="IW781_002662"
     CDS             complement(2216..3151)
                     /locus_tag="IW781_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1183634.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3453..5387
                     /locus_tag="IW781_002663"
     CDS             3453..5387
                     /locus_tag="IW781_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1183635.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5844..6260
                     /locus_tag="IW781_002664"
     CDS             5844..6260
                     /locus_tag="IW781_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1183636.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6274..6840
                     /locus_tag="IW781_002665"
     CDS             6274..6840
                     /locus_tag="IW781_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1183637.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6863..7849
                     /locus_tag="IW781_002666"
     CDS             6863..7849
                     /locus_tag="IW781_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1183638.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8004..>8495)
                     /gene="scm"
                     /locus_tag="IW781_002667"
     CDS             complement(8004..>8495)
                     /gene="scm"
                     /locus_tag="IW781_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1183639.1"
                     /translation="STTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDS
                     SDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKT
                     SSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKK
                     SKR"
BASE COUNT         2491 a         1638 c         1729 g         2637 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
       61 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      121 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      181 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      241 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      301 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      361 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      421 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaagc aaattgtcca
      481 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      541 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      601 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      661 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      721 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      781 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      841 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
      901 tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg aaatacacta
      961 gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat ggttcgtact
     1021 ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga taatttgaag
     1081 gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa gatcgcttcc
     1141 gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc cattgaaaag
     1201 actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt gacacttgcg
     1261 atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta ttttttgcca
     1321 tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt ccgttcttta
     1381 atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac tgtccagtct
     1441 tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa gtcaacgatg
     1501 gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat gtaacctttg
     1561 gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt tttttctttg
     1621 tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat ttcttcttgg
     1681 gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc accaatcgcc
     1741 atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac taacgtgtag
     1801 acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc atcgatctca
     1861 gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc gaaggcaggt
     1921 ttcaatgttg attctttgcc ttcatcatac acacggatac gaacgtaagt ccctaacaag
     1981 aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact ttcagtggtt
     2041 gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc cagtgtgatt
     2101 aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa ggtgagacga
     2161 ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta tgtgattatt
     2221 tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca gctgcattca
     2281 ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct gtaacaactt
     2341 ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg tcgttcagag
     2401 tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca ttatattctg
     2461 tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat tcagttactt
     2521 tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag ttcttttctt
     2581 caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt gtatctgtgt
     2641 taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt tcagatgagt
     2701 gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag aattggtcat
     2761 tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg tttttgttgt
     2821 attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat tttgattcag
     2881 tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt gaataatttt
     2941 tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact tttgcagatg
     3001 attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa gaagagtttg
     3061 agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat gctaatacgg
     3121 caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc atttctagtc
     3181 cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata agtcaactat
     3241 ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt tcgtgaaaaa
     3301 acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga acagaacatc
     3361 tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc atatattcat
     3421 tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt gtcgttgtag
     3481 gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg aaaaaagaag
     3541 atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc gagttgcatg
     3601 aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag catttgtttg
     3661 ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat aaagaacgaa
     3721 aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt ggaatgggcg
     3781 gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc ctttggtcat
     3841 ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa gctgcattgg
     3901 aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt tctggattta
     3961 ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc aaagattata
     4021 aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg atcttgaata
     4081 tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac aacgtggaat
     4141 tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg aaagacggac
     4201 gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact tctgacgctg
     4261 ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa tatatgcagg
     4321 caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt tatgaagaat
     4381 tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga cacacagccg
     4441 cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa ggaaactatc
     4501 aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt gataagatcc
     4561 atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa tacttctttg
     4621 atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc catatcaaag
     4681 atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta tggagtgtgc
     4741 cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa atcattggga
     4801 atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc actggaaaca
     4861 gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt gcttctgctg
     4921 ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa gcagcgactc
     4981 aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct gtgattgaga
     5041 agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat gatgtagcaa
     5101 tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca ttgattgctg
     5161 gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg ttctgcttat
     5221 caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca ttgatgaacg
     5281 gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa cgtacactag
     5341 gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata gataaagaac
     5401 gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac agcctccttt
     5461 ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa gacacaggtg
     5521 tcacaacctc tgttacaacc tcttgttata aacggtgttc cagaagtagc ttcttcgaaa
     5581 aataagccga aatttcacaa aaacttgaaa agtaattttc gtaaattcct tcttatttct
     5641 tgaagttgcc cacttctgtc acaacctctt gttataaacg gtgttccacc tgctgcttct
     5701 cggtaataag cccaaagaat tagaaaacat ggaaagctgt tttctattct tcgttcttat
     5761 tactcaaagc aagacacagg tgtcacaacc tcttgttttt atgctaaaat tgggtgagtt
     5821 aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt tgctgaaaaa agtctgctca
     5881 aaccttggga tgtttttatt attttgcttt tgactgttct ttctttttta ccagtcatga
     5941 tttttggtct tcagcaaaac accgcaacag cagaaaaaga agcggtactg cgtgtggatg
     6001 gcacagaaat caaaacattc cctttgatgg ctggagaaga gagcaacaca tatacttaca
     6061 aagatgaaca cggagattat aacttgatcg agatagatgg cgatcgagta cggataaaag
     6121 aagcagattg cgatgaccag atctgcgtac gtcgaggctg ggcaacgaaa aatggcgaaa
     6181 ccatcgtctg tttgccacat aaattagtga tcgaagtccg ttcaacagat ggaggtgacg
     6241 aagacagtct gatttattag accttcacac accatgagta aattacaaaa aatgatttat
     6301 atttcgctac ttgttgcaca aggagtgatc attggtttat tggaaaatat gatcccttat
     6361 cctttttctt ttgcgcctgg tgcaaaacta ggcttagcca atctaatcac gatcgttgcc
     6421 atttttacca tgaaaaaacg agacagcttc ttactacttt ggctacgact attcctgact
     6481 actttgctag gcggaacgat ttccacattc ctttatagta tgagtggtgc tttgcttagt
     6541 tattttggaa tgttattggt caagcaatta ggaccaaaac gtgtcagtat catcggaatt
     6601 agtgcggccg gcggtttcat gcataatgtc ggacagcttt tgatggcttc tttcattgca
     6661 caatcatgga cagtcatgtt gtacttgcca gtcctttcag ttttagggat tttatcaggg
     6721 attgcaatcg ggatcgcagc gaattatctt ttacgacatg tgcagacact tcaacgattc
     6781 caaaaagatt atgagcaggg aaccaaacaa aaatggagcc caatgtttac caaaaaataa
     6841 tcgtcacaca aggagttttt ttatgaagct acatccaatg tgggaaaact atcctgcttt
     6901 aagtaaagaa ttgacagaaa cactaaaacg gatggaagaa actgtccgcc tgaaaaacaa
     6961 acctgtcgaa caagcaatca aagaaacgat ccatgccggc ggaaagctgt tgcgtcctgc
     7021 ctatcagcta ttgttctcac aatttggacc agagcaagat cgagacaaag ccatcgcttt
     7081 ggcagcttct atcgaaatgc tccatacggc cacactgatc catgatgata tcgttgatga
     7141 agcggatctg cgacgtggtc agcctaatat ccgtagtcag tttggtaatg tcgtttctgt
     7201 ctatgcagga gactatttat ttgtctgctg ttttaaacta ttgtctgatt actccacttc
     7261 cttgaaaagt ctccaattga attctcgcag tatggaaaaa gtattgaatg gcgaattagg
     7321 gcagatggat gatcgctata attatgaact ttctgtaaaa gaatatttgg aaaatatttc
     7381 aggtaagaca gctgaactgt tccaattaag ttgttctgtt ggcgcttttg aaagtggaac
     7441 aagcgagcgc ttcgcaaaaa aagcaggaga tatcggactt tctatcggga tggcctttca
     7501 gattatcgat gatattttag attatacaaa agagagccag gaaatcggca aacctgtact
     7561 agaagacatg cgtcaagggg tctattcttt accgttgatc tactctttgc aaaaaaacaa
     7621 accaaagcta ctaccttatt tagagaagaa agcggcaatg acagaagaag atgtcgacgc
     7681 cgtacgcaaa atcgttgaac atacaggagg cgtggaagaa gctaggaagt tagcggcctc
     7741 ttacacagaa aaggctttga aggaaataaa gaaattacct gccacctctc ttcgaacaaa
     7801 agagaatctc ttttccctta cccaactgat tttggatcgc aaagactgaa tagggaaagg
     7861 ttcgatacgt aaataaattt ctcagaagga aatctacgtg ttttttgaca aattgaattg
     7921 tagttcttgt atttatgata aaaagcaaaa ataaagagca acgtccgtta gcgacgttgc
     7981 tcttttttat cttgaatatt tttttacctt ttcgatttct tataactcat aaaagtgatt
     8041 cttaaaccga ttacaagtaa tataacgcct gcgagtgtta tccaattatt cgattgactt
     8101 ccagttttag gaagtgctct ttttgatttg tttccattgt cattgcttgt tttattagtt
     8161 aagttaaagt tcgtcctgtt atccgaactt gtttttgaga aggagacact tgcatgacgt
     8221 gtatcgcttg tggtagacga cgtgtctgta ctatcgcttg aagttgatga atctgagcta
     8281 tcacttgtgg tagaggatgt atctgtactg tcacttgaag ctgacgtgtc cgtgctatca
     8341 cttgtagtgg tagatgaatc tgagctatca cttgtggtag aggtagtatc tgtactgtca
     8401 cttgaagctg acgtgtccgt gctatcactt gttgtggtag atgtatccgt actgtcgctt
     8461 gaagctgacg tgtccgtgct atcacttgtg gtaga
//
LOCUS       DACEOM010000103         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEOM010000103 DACEOM010000000
VERSION     DACEOM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.107"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..173)
                     /locus_tag="IW781_002668"
     CDS             complement(<1..173)
                     /locus_tag="IW781_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183640.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IW781_002669"
     CDS             complement(593..1714)
                     /locus_tag="IW781_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ1183641.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IW781_002670"
     CDS             2042..3451
                     /locus_tag="IW781_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1183642.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IW781_002671"
     CDS             3448..4176
                     /locus_tag="IW781_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1183643.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IW781_002672"
     CDS             complement(4304..5215)
                     /locus_tag="IW781_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1183644.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IW781_002673"
     CDS             complement(5232..6239)
                     /locus_tag="IW781_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1183645.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IW781_002674"
     CDS             complement(6236..8365)
                     /locus_tag="IW781_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1183646.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IW781_002675"
     CDS             complement(8370..8477)
                     /locus_tag="IW781_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183647.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACEOM010000104         8424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEOM010000104 DACEOM010000000
VERSION     DACEOM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.108"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            471..2108
                     /locus_tag="IW781_002676"
     CDS             471..2108
                     /locus_tag="IW781_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1183648.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IW781_002677"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IW781_002677"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1183649.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="IW781_002678"
     CDS             complement(3184..3819)
                     /locus_tag="IW781_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1183650.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IW781_002679"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IW781_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1183651.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="IW781_002680"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="IW781_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6417..>8424
                     /locus_tag="IW781_002681"
     CDS             6417..>8424
                     /locus_tag="IW781_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1183652.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2799 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaa
//
LOCUS       DACEOM010000105         8339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEOM010000105 DACEOM010000000
VERSION     DACEOM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.109"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            118..402
                     /locus_tag="IW781_002682"
     CDS             118..402
                     /locus_tag="IW781_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAQ1183653.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
     gene            499..2043
                     /locus_tag="IW781_002683"
     CDS             499..2043
                     /locus_tag="IW781_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183654.1"
                     /translation="MVYAYGLRESIWNGFLKDADPLGFENVKSDEFLKAVVATFWERY
                     GGKTYEGLKPKLAIYAAGVEEAATEVKPALERILANLGIPASKILLNVGDAKYTKNDD
                     IRDFNNLDVPGTEGNEKQFIILVEKGKEGWNCRSLFAVALFRSPKSKIFVLQATMRCL
                     RAITDERLTATVFLSKENYDTLDDELHKNFNMEIKDIKNPSNADRHKYQVRVLPPPRT
                     ITLKRVWHEYTLHEKEYSEPVDFKLVDADLSKYASIMYERDSLARDTTVKEKNIDYLQ
                     DNMKFSEFSLAGEVARYLNISCILAARILRESVDGIDTVLDAVNRYNAVLDDYVIPTV
                     FHALFDITSELKTEDKELVLLREPKDAGYYEFSAKDDLVLTNRYPGFTPEQLKKSFHA
                     DTYCFDSIPEKECFMQYITSGKVAEVYFTGMFTSNQGDLSVHYYDPESGRIRQYYPDF
                     LARMTDGSYQLIEVKGDNKIDDTVVKAKQAAAEEMAVASGVKYLMYAGSQVVSTHILD
                     DDSASM"
     gene            2302..3225
                     /locus_tag="IW781_002684"
                     /pseudo
     CDS             2302..3225
                     /locus_tag="IW781_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
     gene            3218..4462
                     /locus_tag="IW781_002685"
     CDS             3218..4462
                     /locus_tag="IW781_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1183655.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            4476..7625
                     /locus_tag="IW781_002686"
     CDS             4476..7625
                     /locus_tag="IW781_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1183656.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2834 a         1511 c         1766 g         2228 t
ORIGIN      
        1 aagttacacg ttactaaagg gaatgtagat aaattattag gtatactact gacagcttcc
       61 aaggagctaa agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt
      121 cccactaagc gctcgggacc ccttgtagga aaatgtccta agtgtggcaa caatattgta
      181 ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat gtaccttcac tttagctgaa
      241 cattttagaa agaaaaaact caccaaaaca aatgtaaaag aattactaga gggaaaagaa
      301 accctggtaa aaggaatcaa aacgaaagat agaaagtcct acaatgccgt tgtaaaaatc
      361 ggagaaaagg gatatattga ttttatctct ttttcaaaat aagcataaaa gccctttaaa
      421 gagggctttt atatattaat cacaaatcac ttatcacaaa tcactacgcc gtatgtaaag
      481 aaccagcttc tgccggaggt ggtatatgcc tacggtctgc gtgaatccat ctggaacggt
      541 ttcttgaaag atgctgaccc gctcggtttt gagaatgtta agagcgatga gttccttaaa
      601 gctgtggtag ccacgttctg ggagcgttac ggaggtaaga cctatgaagg cctgaagccg
      661 aaacttgcta tctatgcggc aggcgttgag gaagcggcca cagaggtcaa gcctgcgctt
      721 gagaggatac tcgctaacct cggcattcca gcatcgaaga ttcttctgaa tgtcggcgat
      781 gcaaagtaca caaagaacga tgacatcagg gacttcaata accttgatgt tcctggaacg
      841 gaaggaaacg agaagcagtt cattattctc gttgaaaaag gcaaggaagg ttggaactgc
      901 cgctctctat ttgctgtggc tcttttccga agcccgaagt caaagatatt cgttctccag
      961 gcaacaatgc gctgcctccg cgctataacg gacgagcgtt tgacggccac agtatttctc
     1021 tccaaagaga actacgacac cttggatgat gaacttcata agaacttcaa catggagatc
     1081 aaagacatca agaatccgtc caatgctgac cgccacaaat atcaggtccg tgttctgccg
     1141 ccgccaagga caattacact caaacgtgtg tggcacgagt ataccctcca cgaaaaggaa
     1201 tactccgagc ccgtggactt caagttggta gatgccgacc tctccaagta tgccagcatc
     1261 atgtacgaac gtgacagcct tgctcgtgat accaccgtca aagaaaagaa tattgactat
     1321 ctccaggata atatgaagtt cagcgaattt tccctcgcag gagaagtggc tcgttatctg
     1381 aatatttcct gtatccttgc cgcgagaatt ctccgagaat cagtggacgg aatcgacacg
     1441 gttcttgatg cggtcaatcg ttataacgct gtcttggatg actacgtcat cccgactgtg
     1501 ttccatgcgc tctttgatat tacctcggaa ctgaagaccg aggataagga actggtactg
     1561 ctacgtgaac caaaggatgc cggttactac gaattctccg caaaggatga ccttgtgctt
     1621 accaatcgct atccgggctt tacgccggag cagcttaaga agagcttcca tgctgacaca
     1681 tactgcttcg attccatccc agagaaggaa tgcttcatgc agtacatcac gagcggtaag
     1741 gtcgcagagg tgtatttcac gggtatgttc acctccaacc agggcgatct ctccgtacac
     1801 tactacgatc ctgaatccgg cagaatccgt cagtattacc ctgatttcct cgcaaggatg
     1861 acggacggat cctatcagct gattgaggtc aagggggaca acaagattga tgatactgtg
     1921 gttaaggcaa aacaggcagc cgccgaagag atggctgtgg caagcggcgt gaagtacctc
     1981 atgtatgccg gcagccaagt tgttagcacc catattttgg atgatgattc agcgtctatg
     2041 taaaatgttc tttattgatt gacgaaaaaa cgggggatga caacctttta atttttccac
     2101 accttttaat tattccaaaa tcactacacc gcaagactgt cactccgcta cattgccgag
     2161 cggcagcagg acaactgaac acataaaaca agggcttccg aagtcagtgc gtgacctcgg
     2221 aagcccttat ttcaagcctt tttcggcggc ttttgccacg gagagggctt tttcttttgt
     2281 atcaaaatca gtaggaacat agatccaacc atgggttcag gttcattgat gttaaatgtg
     2341 cgtaactact taactcaccc agataacgtt aaatatcatg ggcaagaatt aaatacaacg
     2401 acatacaatc tagcaaaaat gaacttgatt ttacatgggg tagatgccga agaaatgaat
     2461 ctccgcaatg gggatacgtt gaataaggat tggccaacag atgaacctta cacatttgat
     2521 gcagtagtaa tgaacccacc ttattcggct aattggtcag cagatacgac cttcttagac
     2581 gattctcggt ttaatcgtta tggaaaatta gcgccaaaat ccaaagcaga ttttgcgttt
     2641 cttcttcatg gtttctatca tttgaaggaa acagggacta tggccatcgt cttgccacac
     2701 ggagttttat tccgtggcgc tgcagaaggg gtcattcgtc aaaaattact agaagacggc
     2761 agtatttatg ctgtgatcgg tatgcctgcg aacttgttct tcgggacatc gattccaacg
     2821 acggtgattg tattgaaaaa gaaccgccaa actcgagacg tattattcat tgacgctagt
     2881 cgagaatttg tcaaaggaaa aaatcagaat aaattatcgg aagaaaacat tcaaaaaatc
     2941 ctcgaaacct atgctgaaag aaaagatgtc gagaaatacg ctcatttggc aaactttgat
     3001 gaaatcaaag aaaatgacta taacttaaat attccacgct atgtagatac gtttgaagaa
     3061 gaagaaccaa ttgatatggt tcatgtcgga aatgacatca agaagatacg acaagaacag
     3121 caagtgctag aaaaagaact tttagaagct atctcttccc tgcaaacaac acctgaaaat
     3181 gaagcgtggt tacaaggtgc gctggaggtg ttcaaacatg agtaatgata tacaaccaga
     3241 aattcgtttc ccaggattta cggaagcttg ggaacagcgt aagtttgaat gcttacttga
     3301 taaaaaagat ggagtaagga ggggaccatt tgggagtgcc ttaaagaaag aattctttgt
     3361 atctaatagc aattttgttg tctatgaaca acaaaatgct atttatgata attacgaaac
     3421 aagatataaa attactgaaa aaaaatataa tgaattaatc aaatttaaat tagaaccagg
     3481 cgattttatc atgagtggcg ctggaacaat tgggagaatt tcaagagtac caaagcaaat
     3541 aaaaccgggt gtattcaatc aggctttgat tagacttaga ataaataaag aaatcacaga
     3601 ttcagaatat tttattcaat ttattcgtgc agattttatg caaagaaaac tcacaggagc
     3661 taatccaggt tcagcaatta caaatctcgt accaatgtca gaagtgaaaa aatggatagt
     3721 acaatttcca atattagaag aacaaaagaa aattgggaac ttcttcaaac aactggacga
     3781 cactatcgct cttcaacaac gtaagctaga tttattaaag gaaacgaaaa aaggattctt
     3841 acaaaaaatg ttccctaaaa acggagcaaa agtgccagaa attcggtttc ctggatttac
     3901 ggaagattgg gaacagcgta agttcaaaga gtttagtaag aagactggaa aaaagaacac
     3961 gaaagattta gacttccctg cctattcggt aagtaataaa gcaggtttaa tttctcaaac
     4021 agaacagttt gatggtagta ggttggatga tttagaaaaa acaaattata aattcgttga
     4081 accaaatgaa ttcgcctata atccagcaag agtaaacgta ggatcaattg catttaacaa
     4141 tcttggtatg acggtcattg ttagctctct ttatgtagtt gtgaaaatga gcgaggactt
     4201 agataatgag ttcatacttc agtttatcaa gtcaccaaca tttatcaaag aggtcaagcg
     4261 caacactgaa ggaagcgtac gagaatactt attctatgag aatttcgcta atatcaagtt
     4321 tccttttact aggaataaag aggaacaaca aaaaatcggc gctttcttca aacaactcga
     4381 cgacacgatc gctcttcatc aacgtaagtt agatttgctg aaggaaacga aaaaaggctt
     4441 cttacaaaag atgtttgttt agggagggaa gataaatgaa aattagtcac cgtgaggaag
     4501 cggaagtcga agaacagttg atccgtgtat tgggggaagg gcataatcag tggacgtatc
     4561 gtcccgatct aaaatcagaa gaagatcttt gggtcaattt acgccaaaaa atcatctcaa
     4621 ataatcaagc agaactgaat gattttcctt tgaccgataa agaatttgaa acgatcaaaa
     4681 cagaattgtt actacgaacg aaaaccccct ttgatgctgc gaaatggtta aaaggtgaaa
     4741 atggaatggc tcggatcacg attgaacggg aagatccgca actgggttct gtctcgctca
     4801 ttctttattc taatcaagac attggtggag gaatctctac ctatgaagtg gtccatcaaa
     4861 ttgcaaaacg tggaagtaac atagaagcac gggatcgacg ttttgacgtc accttactga
     4921 tcaatggatt accgattgtc caaatcgagc tgaaacaagt aaccgcaaaa gatagttttt
     4981 atcaggcgtt taaccaaatc aaaaaatatg cagaagaagg tatgtttcgg aacaatatct
     5041 tttcgacctt acagttgttt gtggtttcca atgaacaaac cactcggtat tttgcaaatg
     5101 ccttaccaaa ggatttgcat cctaagtttt tatttagttg gcgaacgaaa gataataaaa
     5161 aggtagaaaa cttatatgag ttttgcaaac aagtgctaaa tatcccggat gctcatcgct
     5221 tgattgcgga ttatacgatt gtcagtgaag atcaagacaa taaaatcctc atggtgttac
     5281 atccttatca agtacatgcg attcaagcct tgtttattgc tgcaaataaa catcaatcag
     5341 gatatgtgtg gcatgcgact ggttcaggaa aaacgttgac tagttttgta tctactaagc
     5401 ttttagcacg aaaatcgggg atcgatcgga cgatcatgtt agtggaccgc aaagatttag
     5461 ataatcaaac caccacagag tttacgaaat ttgcttcgga atttaatact gggatttctt
     5521 ccgggaacgc caaagcgaat agcttgatcg tgggaacagg aagtgccaaa gagttaagtg
     5581 aaactctgtt ggcagatgcc aatgcaaatg ttgtgattat tacaacccgt caaaagctag
     5641 atgcggcgtt gaaatatgca aaaaaacaag aagagaaaaa aggaaccaat cgcttccaaa
     5701 aattaatggg gcaacatatc gtgtttgtag tagatgagtg tcatcgagcg ttaagtgctg
     5761 aaaatatgga agaaatcaaa aaaatgtttc cgaaatccac ttggtttggc tttacaggta
     5821 caccgatttt ccctgagaat cggaaacaag ccaaagggca attggcacgt acgacccatg
     5881 atcagtatgg agaggtcttg catacctata ccatcaagaa tgcattagaa gatggctcgg
     5941 ttttaggatt tcaagtagag catgaaaaca ccgtggagcc gacttcatta gagaataaaa
     6001 tctatcggaa attaaaagaa gtagaaacat atgcagagta ttcaccggaa cagatcaatc
     6061 gtatgattga tcaaatggag cctgtcaaaa aagaaagtta tttggaccct actgtttttg
     6121 aagcagatga acatatccaa aaagtcattc ataaaatgtt ccgtccagac aatgcctata
     6181 caaaatttga ttttcgaaat ggccgtccaa cgaagtctgc gatcttaacc acgagttcca
     6241 ttgacatggc caaaaagtat tatcgagcga tcaaagaaat gacgaaagaa cctgactggt
     6301 taacaaaaga gtttcctaat cagcccattc gtgaaggaag aacgatggag gatcctgatt
     6361 ttccacgaat cgctattacc tattcattag aagaaaacag ccgagattca agtgtccaac
     6421 aagaagaaat gcaaaaaatt attgaggaat ataatcagta ttatgatact tcctggtcat
     6481 tagcagacat tgagcgttat aacggagaca tcaataatcg tttggcacga aaaagagcgg
     6541 aattcaaaca atttgggaaa caaattgatt tagtgatcgt agtggatcgt ctattgacag
     6601 gttttgacgc acctacgatt caaaccctat ttgtggatcg taatctagag tatgcaggat
     6661 tgatccaagc tttttcacgt acgaaccgta cgtatccaga aaaaacaaaa ggactaattg
     6721 ttactttccg aaagccagcc acaatggaga agaatgtcga ggatgcgaca aaactgtatt
     6781 cagaagcaaa agaagaatcg ggtttgattt atccaagtta tcaggaatcc aaaaaaagat
     6841 tcaagcaagc atatggtaag ttaaaagagg tttcatccat ggaatccatt gatgaacata
     6901 cgccacttga aatccgtatc gagtacgtca aagcattcca agaattgaat cgtgcttatg
     6961 aatcgttagt gacttatgac gattacaatg atgacatggt gaaatcttca accttgaaca
     7021 atcaaattca acttttggaa gaacaaattg gcgtgtacga gaccgtgaag ggttcattaa
     7081 tagaggaaga accagaaaaa gagggagaag atttttccac gatcgaattt tatagtgaaa
     7141 ataccacgaa gttgtatgat attgattcta cttatattga tcaattacta ggaacttatg
     7201 cggcgaatag ttcagatgca cgagaagaaa ttgaaaaagc tttggctaaa ttgaataaaa
     7261 cagaaggagt caaagaagtt taccgacaaa ttttaaatgc gtttgatgtg gggactttag
     7321 atagtaatga agatatcttt gtcattaaac gtcgctattt tacacaagcg agtgatgatt
     7381 tgattcatgt cttttccaat gagtggtttg tttctgagaa agaattacat gcttctaatc
     7441 tgcaatatat gccaggtgaa gatccaattc ctaatatgaa agcaattatc aacagtaagg
     7501 attacgaagg atataaagcc aaccatccag aagcgaagcc atttaaatac ccacaagaaa
     7561 tgaagcgagc atggcgtaaa atgttagatg atgagctgat tccacttgaa aatgaattga
     7621 ggtaaacgag aagagttaag aaaattgtac gattcaaaga tggtagtaaa gtggaagtgt
     7681 agaaaatagg gatagatgtc ttttatttgt tcacatttta taacgaagta tttaagacag
     7741 ctattgaata atgtttcctc cacctttttc attcatttac cccaccccgt tgttatatga
     7801 ccgtgttttg agaggaacca ataaaaagca taggctaact aggggttcct ttttcttttg
     7861 tttttttgga atccgcttaa gtgaaatgta tctgaaatga aacggagata aagtgatttt
     7921 ttgctgtaat gacaaggttt tagctgtttc agataccatg taacccaaaa tagtttattg
     7981 agaaaatagg agagtatttg atactaacta gtaggaaaaa atgagataaa aagtaaatgg
     8041 aatcatttca ggtaatctca aataaaaaaa tatacagtcc tgtggcactt tacctatggg
     8101 actgtatata ttttttactc tcagcgggct tgttttgact ccgtgtctga ctccgtcgta
     8161 ttgattttaa atgatattcg atgaccattt aacaggtcat ttttgaaatt tttgtctcta
     8221 tatgactcag tttgataatt attgatagat caatttgggt ctatatcgaa caagggaata
     8281 atccaataaa aaatgattta gaatatcaaa ataacaaaaa agaggactac ctgtattag
//
LOCUS       DACEOM010000106         8145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEOM010000106 DACEOM010000000
VERSION     DACEOM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.110"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            205..618
                     /locus_tag="IW781_002687"
     CDS             205..618
                     /locus_tag="IW781_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183657.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(611..790)
                     /locus_tag="IW781_002688"
     CDS             complement(611..790)
                     /locus_tag="IW781_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAQ1183658.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITEDSQVIDMTK"
     gene            1289..1513
                     /locus_tag="IW781_002689"
     CDS             1289..1513
                     /locus_tag="IW781_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183659.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIVEMTREAT
                     PNEIDKCKLIYCGHGYFSEQNIQTNIKKYH"
     gene            1539..1994
                     /locus_tag="IW781_002690"
     CDS             1539..1994
                     /locus_tag="IW781_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAQ1183660.1"
                     /translation="MSKLNPKQQAFADEYIITGNAYQSALKAGYKENYAKNAQEKLVE
                     KGGKVSDYIQEKLKEVQTKRHLTMEEALAITASIAKGEPQRFEVVKRDPYTNEIIERE
                     VSEYSAGFKERNQALEHYYKINAAFVDKQKVEISEIPTFIDDISSDDDG"
     gene            1987..3285
                     /locus_tag="IW781_002691"
     CDS             1987..3285
                     /locus_tag="IW781_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAQ1183661.1"
                     /translation="MAKKLSEFLPPKFHSVWRATLNQDILNIVCKGGRGSGKSSDIAH
                     IVTQLLMRYAVNAVGIRYVDNTLEQSIYEQMKWAIEKQGVSRLFKFNKSPLKITYLPR
                     GNYMIFRGAQNPERIKSLKDSKFPFAIGWIEELAEFKTEDEVTTITNSLLRGELDDGL
                     FYKFFYSYNPPKRKQSWVNKKYETSFQPDNTFIHHSTYRDNPFISKEFLKEVEATRER
                     NPRRAEWEYDGKAVGSGVVPFDNLQVKKGSITDDMVANFDNIRNGLDYGYATDPLAFV
                     RWHYDKKKNGIYAIDEIYGVKISNREFANKAKSKGYQNEEIFSDSAEPKSNAELVNEH
                     GMKGIKGVKKGPDSVEYGEQWLDDLAFICIDPLRTPNIAKEFENIDYQTDRDGNPKPR
                     LEDKDNHTIDATRYAFNEDMWAKKKSTVTKEQRNKIRRMF"
     gene            3298..4797
                     /locus_tag="IW781_002692"
     CDS             3298..4797
                     /locus_tag="IW781_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1183662.1"
                     /translation="MDKVNEFEHGSDIHYSSDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHKYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKDRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRIIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQTGIFDSDVEEVINNKKDETGE"
     gene            4802..5728
                     /locus_tag="IW781_002693"
     CDS             4802..5728
                     /locus_tag="IW781_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1183663.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTITSSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLLRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            5855..6457
                     /locus_tag="IW781_002694"
     CDS             5855..6457
                     /locus_tag="IW781_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1183664.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPTEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELEAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            6470..7393
                     /locus_tag="IW781_002695"
     CDS             6470..7393
                     /locus_tag="IW781_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1183665.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRSA
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRVTMDVGFMIAKDEAFGKISPKA"
     gene            7411..7674
                     /locus_tag="IW781_002696"
     CDS             7411..7674
                     /locus_tag="IW781_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183666.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAATVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            7686..8015
                     /locus_tag="IW781_002697"
     CDS             7686..8015
                     /locus_tag="IW781_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAQ1183667.1"
                     /translation="MTIVEDIKKLLKGTLDEKLEVIERRTNERMKTLLNTKEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFI"
BASE COUNT         2962 a         1270 c         1711 g         2202 t
ORIGIN      
        1 gaatttatcg taataacttt tgagtaaata gaacaaaaaa cctgcactag tttccgctag
       61 tacaggcagt gactatatcg gttgataatc tagcatatat taaaaataat tgcaagaaaa
      121 gaaatttata gtggtcgata ccaaattatc aaagcaatgt tattttgttg ctgtctatta
      181 atttatgtat tggaggaaaa acgaatggat aaagaaatca aagtaactgt caaattagat
      241 ttgactgaac taaaagaact gctcaacaag gctagtgacc aagtcgaaca aataaagtat
      301 caaggtatgg atcgaaaacg gttgattgat attattgagc aacaacgttt agaattgatc
      361 caaaaagaag ctgtatgtaa agagtacaaa aaacatttag aacaagtaat tgagtatcat
      421 tcagtagaaa agtacagatc agtggtgcaa aaaaatagag caacgagtcc tgatcctcgt
      481 caagatgtat cgattagtat caagacgcca gtatttaatt ctgttaaatc agaaaagcta
      541 gaaagtatct caactgcttt ggtagaaaaa gcagctgaaa tgaactccct agcaaatgaa
      601 gttagtcgtc ttatttagtc atatcgataa cttgagagtc ttcggtgatt ccgtttttta
      661 tagaattaat ggtttgttca gcggaatatt tagtcaagta tgtttcgcta gttgctacta
      721 cttcattgtt attggactta ataacgaagt aatattggcc attagttgct tctcttataa
      781 caaagtacaa atttttcacc accttttgat ttatttcagc ggaccactcg ctgataacta
      841 aaattatacg cttagtattt attttcacaa tattaatttg tcactgtggc ggaaaggtaa
      901 cgctaataac ttaaatcagt ggtaatgaac acctgagctt agtttatgca gctaggcaag
      961 agaccgtcga gggtaaaggg caagaggtat cttgaaataa gtcccctaaa gatagacaat
     1021 tctagaggtg agagttttta tgatggagag tctgtaaaat atcggaaaat ggtgttgcaa
     1081 ggttcgattc cttgccagtg attttagcaa ccgagggttg gaaatgggcg ctcaaagtac
     1141 acgagcaagg cgaggtcgat agtaatcgat ggaatcggtg taggttgctt gatagagcta
     1201 taactgcatc tcattgttga gatgtagttt ttacatatta gatcactcgt tgagtggtct
     1261 ttttattttg cgtaaaggag actgcataat gaggaactac tggtatgtat cattatcaaa
     1321 taaatatccg caaccaaacg cagatgatcc aattagagtt gtccaatcag tccagattaa
     1381 aaagaagtac tccattgttg aaatgaccag agaagctacg ccaaatgaaa ttgataaatg
     1441 caaacttatt tattgcggtc atggatattt tagtgagcag aacatacaga caaatataaa
     1501 aaaatatcat taacatataa caaaggtggt gatggaaaat gagtaagttg aatcctaagc
     1561 aacaagcctt tgctgatgag tacatcatca caggcaatgc ttatcagtca gcactgaaag
     1621 ctggctataa agaaaactac gctaagaacg cacaagaaaa attggtggaa aaaggtggaa
     1681 aagtatccga ctacattcaa gagaagctaa aagaagttca aactaagagg catttaacaa
     1741 tggaagaagc tttggctatt actgcttcta ttgcaaaagg agaaccacaa cgctttgaag
     1801 ttgttaagag agatccttat acaaacgaaa tcatagaacg tgaagtgagt gaatattcag
     1861 caggtttcaa agaacgtaac caagcacttg aacattatta taaaataaac gcagcatttg
     1921 tagataagca gaaagttgaa atttctgaaa tacctacttt cattgatgat ataagtagtg
     1981 atgatgatgg ctaaaaaact atctgaattt cttccgccga agtttcattc agtatggaga
     2041 gcaactttaa atcaagacat tcttaatata gtttgtaaag gtgggcgtgg ttcaggaaaa
     2101 tcatcggata tagcgcatat cgttactcag ttacttatga gatatgcagt gaatgctgta
     2161 ggtatacgtt atgttgataa tacacttgag caatctattt acgagcaaat gaaatgggca
     2221 attgagaagc agggagtatc gcgcctattt aagtttaata agtcaccact taaaattacc
     2281 tatcttccaa gagggaatta tatgatattt cgtggtgctc aaaacccaga acgaatcaag
     2341 tctttaaaag atagcaagtt tccatttgct ataggatgga ttgaagaatt agcagaattt
     2401 aaaacagaag atgaagtcac gactatcacg aactcccttt tacgtggaga gttagatgat
     2461 ggtctttttt ataagttttt ttacagctac aacccaccta agagaaaaca atcttgggta
     2521 aataaaaaat atgagacttc ttttcaaccg gacaatactt ttattcatca ctcgacctat
     2581 cgagataatc cattcatctc taaggaattt ctgaaagaag ttgaagcaac tagagaaagg
     2641 aatccaagaa gggctgagtg ggaatatgat ggaaaagctg tggggtcagg agttgtacct
     2701 tttgataatc tacaagttaa gaaaggttct attacagatg atatggttgc taactttgat
     2761 aacatccgca acggtttgga ctatggatat gcaacggatc ctttagcgtt cgtcagatgg
     2821 cattatgaca aaaagaaaaa cggtatttat gcaatcgatg aaatttacgg cgtgaagatc
     2881 agcaatcgag aatttgcaaa caaagctaaa tctaaaggct accaaaatga ggagatattt
     2941 tcagatagcg cagagccaaa gagtaatgct gaattagtta atgaacatgg tatgaaagga
     3001 ataaaaggcg tgaaaaaagg acctgattct gttgagtacg gtgaacagtg gttagatgat
     3061 ttggctttta tttgtattga tccactacgc actccgaata ttgctaagga attcgagaac
     3121 atcgactatc aaacagatcg tgatggaaat cctaaaccaa ggttagagga taaagataac
     3181 catacgattg atgcgacaag atatgccttc aacgaagata tgtgggccaa aaagaaatca
     3241 accgttacta aagagcagcg gaataaaatc agaagaatgt tttaaggagt gtgagaaatg
     3301 gataaggtaa acgagtttga acatggatct gatatacatt attctagcga cgtaaacaca
     3361 aattatgtaa agtttagcgt agactcaaat cttcactata gatttagctc agcagaagat
     3421 ttacttaacg atttagatac tttagcagca atgataaaac atcatcataa atatcaggta
     3481 aagcggctta gtgtattaga tgattattac aaagctagaa atacaaatat catggataac
     3541 cgtagacgta gagaaaagga aaaagcggat caccgatcag cacataactt tggaaaagtt
     3601 ctttgtacgt ttgatgttgg gtacaacaca ggcaatccta taaaagtgca aatcgaggac
     3661 acaaatcaac aaaaagaaat cgaagagttt aatactaata atgacataga tgggttaaat
     3721 gctgaactct ggcttgatat ggataagtat gggagagcct atgagattat ctatcgagat
     3781 tcagatgata cagattatgt tgatttggct aatgtatttg aaacgtttgt tgtatatgat
     3841 actacagtaa aacgagagcc tattttggct gtacggtatc ctaagacaag attcaacaag
     3901 gatgctgata aacagtacat tcaaccaatc gtatacacaa aagaaaaaag tatcacttat
     3961 gatgagacga cactaacagc aattgagtta aaaaatcccc aggatgaacc gcatgaatat
     4021 aaagaggtac ctattacaga gtattctcct aatcgttttc ggatgggctt gtatgaagat
     4081 gtactatctt tgattgatct atacgatgca gggcagtctg ataccgccaa ctatatgact
     4141 gatctaaacg atgctcttct agttattagt ggcgatattg aagcagcagg actatccaca
     4201 gaggacgcca tcaagcagaa agaagcgaat atgcttttgc ttgagtctgg aactgatgtg
     4261 aacggtaata aaacaagtgt gactgcagga tatatttaca aacaatatga tgtgaacggt
     4321 gtagaagcat acaaagacag agtgcgcaag gatatccacg aaatctcaat ggttcctgat
     4381 cttactgatg acaatttttc cggagtgcaa tcaggagaag caatgaaata taaattattt
     4441 ggatttgaac aaatgacggc aacaaagcaa aggctattca aaaaaggcct tatgcggcgt
     4501 tatcgtcttt tatttagcct aaaatcaagt atttctgaaa tggataactc cgatttgaaa
     4561 ggcttacgta taatatttac gcctaatcta cctaaagcca ttctggaaga gttgaaatct
     4621 ttggttgatg ctggagctga actcagtcaa gagacgatct taggactcgc ttcttttgtt
     4681 ccagatgtac aggcagagtt gaaacgagta aataaagaaa cgcaaaagca gactggtatt
     4741 tttgattcag atgttgaaga agtaattaac aacaaaaaag atgaaacagg ggagtgatta
     4801 aatgaactcc caagaatatt ggatcaaacg ggaaaaagaa tggcaaaagc aacaaattaa
     4861 agatgataaa aagcgcatgg cagaaattaa aagtcgcatg caatacgcgc aagatgcgat
     4921 acaaaaagaa atagacgcgc agtgggatag tttctccaat ggtcagaaaa tcactcgtag
     4981 cgaagcgatg aagcgtgcta gtgaaatgga tgtcaaagca ttcgctcgca aagcaaagaa
     5041 gtatgtcaaa gagaaagatt tttctcctac agcaaaccaa gaattaaagc tatacaatct
     5101 tacgatgcgt gtaaatagat tagagctctt aaaagctaat atcgggcttg aattgatttc
     5161 actgtttaat gaattggata agtacttttc gaatgaatta acaaaagcgg gtttagctga
     5221 attgaagaga caagccggta ttttagaaat gactattact tcaagtggat atgcaaagct
     5281 gatagaacta gtaataaaca gctccttttt gagtgatgac gtgtctttta gtgatcgctt
     5341 atggatgtat caatctgaat tgaaatcaga attagatagg ttgttactga gaagtataac
     5401 gatggggaaa aatcccaagc aacttgcatc taaattggca gaatatttaa cagctgaagg
     5461 acgagaaaac actaagttca acactcaacg tttgatggtg actgaaacga ctagagttca
     5521 ggtaggaatc caagaacgaa gttacagaga tgcaggcatt acccagtaca tctatatagc
     5581 agaaccaaca gcgtgcaaac tatgtatacc gttaaataat caagtttttg atgttgccga
     5641 tatgcagccg ggaagtaacg ctcctaacat gcatccattt tgtcgatgca gtacagcacc
     5701 ttatatagaa cgaatatcaa gtcgttaata caaattaacg gcttcttatt gtgccttctt
     5761 acagcttaca ggcgttaaag agaaagctat tttcggctga ccggcgtaac tggtcaaatt
     5821 tatcgggtag cggcgtaacc gtggaggatt aatcatgaaa aaacgtttat ttatgccaat
     5881 gaacttacaa tttttttctg aaccaggaga tggtggatct ggtgatgagg gacaacaagg
     5941 aaacctacca gctggctcac aagagacacc gaccgaagca aaagaagaaa acaatactgg
     6001 caaaacattt tctcgtgatg aagtagcgaa aatgatcgct gctgagacga ataaagcaaa
     6061 agcagcgtgg gaaaaagaac tagaagcaaa aaaagaagaa gctaaaaagc tggcaaaaat
     6121 gaatgcggaa gaaaaactac agcatgagtt ggaacaaaaa gaagctgaaa tcgctgaatt
     6181 aaagcgtgga caggcactat ctgaaatgac gaaagaagct tctaaaatgc tgacagatgc
     6241 aaatttacca cacgatgatg atttacttgg tctgattgtt tctgatgatg cagatgccac
     6301 aaaacaagct gtagcagtca tcactaactt tgcttctttg attaagagag aaaacgcaag
     6361 acaaacacca ccaaatgaag gtggacaatt tacagcatcg aaaaatacta aagaaacagt
     6421 ggctaaacta gctgctaaaa atcgaattat caaataggag gaaaacttaa tgaaaaagaa
     6481 acaactttta ccaatgaact tgcaaatgtt tgctcaaaca tgggatccag ataatgtctt
     6541 ggtatatgaa acgaaagagg ggaaaattcc tgataaatat aatacgctca ttttgagtga
     6601 agttatggaa aattctaaga tcatgcagtt agcaaaatac gaagaaatga ctgacaaaga
     6661 aaagaaattt gaatactttg cagaaggacc aggcgcatac tgggtgggtg aaggtgaaaa
     6721 aattaaaacg tctaaaccta aatggatgca agccacgatg actgcaaaaa aactcggtgt
     6781 cattcttccg gtttctcgtg aatatttaaa ttataaatta tcagatttct ttgaggagat
     6841 gcagccaaaa attgctgaag ctttctataa aaaatttgat gcagctgcct tattaaataa
     6901 agaaaaccca tttcctcagt cactagacgg atcagttatt agtgcgggga atgtggttga
     6961 aggcggattg acttatgata atatcctagc cttagaagac aaattagcag aaaatgaatt
     7021 tgaacctaat gcgtttattt caaaccgaaa aaatcgtaca gaattacgtt ctgcagctca
     7081 aacagtaggg tcaaatgttg agtttattta tgatcgctct gctaatacaa ttgacggatt
     7141 accagtagta gaccttaagt ctttagataa aggaactctt tacgctggag actttaatta
     7201 catgttttat ggaatcccat ataatatttc atttaagatt tctgaagaag cccaattgtc
     7261 tactttaact aatgaagatg gaaccccagt taacttgttt gagcaagaac tgattgcttt
     7321 gcgtgtaaca atggatgttg gatttatgat tgcaaaagat gaagcatttg ggaagatttc
     7381 cccaaaagcg taacgcctgc taccggtatt gtgccaaatc aaaagacatg gaccggtaaa
     7441 gtaggcgata ctaaaacatt tactatttca gctgtgcctg cagatgctag cgatgcagct
     7501 actgttgttg cagctactac agcaacttca agtgatggag ctatcgcaac agtgaccaaa
     7561 aatgaaaatg gtggttttga tggaacgatt gcagcagaag gatcagcaac attcacattt
     7621 acttctggag aattcactac ttcaatcaat gtgacaggtc aacctgctag ttaggaagta
     7681 aaaatatgac gattgtagag gatattaaaa aacttcttaa aggaacacta gatgaaaagc
     7741 ttgaagttat tgagcgaaga acgaatgagc gtatgaaaac cttgttaaat acgaaagaag
     7801 ttcctaaaga atttgaaaca gttgtatatg aagtatcgtt gaaaagattc aatagaattg
     7861 gtcaagaagg tatgcagtca tattctcaag aaggtttatc tatggctttt cctgattcgg
     7921 atttttcaga gtatcaaaat gagattgacg aatttaagcg taaagatcag gaagagttgt
     7981 acaagccaaa gcgagggagg tttaaattta tatgagattt acagatgaaa ttatatttgt
     8041 taaacgttca tctgactcta aatatgatcc agatctcggt gagtgggttg aaggcaaacc
     8101 agaaagaaca agaacagagg caaacgtgac agatattggc actga
//
LOCUS       DACEOM010000107         8077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEOM010000107 DACEOM010000000
VERSION     DACEOM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.111"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..538)
                     /locus_tag="IW781_002698"
     CDS             complement(17..538)
                     /locus_tag="IW781_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1183668.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="IW781_002699"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="IW781_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1183669.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="IW781_002700"
     CDS             4618..5505
                     /locus_tag="IW781_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1183670.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="IW781_002701"
     CDS             5502..6671
                     /locus_tag="IW781_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183671.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="IW781_002702"
     CDS             6695..8050
                     /locus_tag="IW781_002702"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1183672.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2598 a         1491 c         1830 g         2158 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaa
//
LOCUS       DACEOM010000108         8012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEOM010000108 DACEOM010000000
VERSION     DACEOM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.113"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..376
                     /locus_tag="IW781_002703"
     CDS             <1..376
                     /locus_tag="IW781_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1183673.1"
                     /translation="INPDFIETVNYVTNFQREMRKVEAMTVLATQSPQEMVPENMSSD
                     NFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKSMTNSELESIPQQNQGDAVINFGS
                     KETIRVHFSPNKRQLKLFDGGK"
     gene            376..1581
                     /locus_tag="IW781_002704"
     CDS             376..1581
                     /locus_tag="IW781_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1183674.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMNF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAMKHWGEGGNDNGTITGEWTNPFPGSSLDKSTFSGGQLFGTNPGS
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            1597..2241
                     /locus_tag="IW781_002705"
     CDS             1597..2241
                     /locus_tag="IW781_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183675.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNSSSAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVNEAQSKTAEEFG
                     KLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2252..3058
                     /locus_tag="IW781_002706"
     CDS             2252..3058
                     /locus_tag="IW781_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183676.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMIIGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            3081..3310
                     /locus_tag="IW781_002707"
                     /pseudo
     CDS             3081..3310
                     /locus_tag="IW781_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3469..4404
                     /locus_tag="IW781_002708"
     CDS             3469..4404
                     /locus_tag="IW781_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1183677.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            4448..4717
                     /locus_tag="IW781_002709"
     CDS             4448..4717
                     /locus_tag="IW781_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183678.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            4949..5245
                     /locus_tag="IW781_002710"
     CDS             4949..5245
                     /locus_tag="IW781_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183679.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            5247..5669
                     /locus_tag="IW781_002711"
     CDS             5247..5669
                     /locus_tag="IW781_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183680.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            5688..7280
                     /locus_tag="IW781_002712"
     CDS             5688..7280
                     /locus_tag="IW781_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183681.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            7301..7636
                     /locus_tag="IW781_002713"
     CDS             7301..7636
                     /locus_tag="IW781_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183682.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            7782..7976
                     /locus_tag="IW781_002714"
     CDS             7782..7976
                     /locus_tag="IW781_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183683.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT         2996 a         1184 c         1649 g         2183 t
ORIGIN      
        1 gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg
       61 taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa
      121 tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat
      181 ctttatgcgt atggatgaat ctattttacg tcatatagaa ggattagttg gtaaatcaat
      241 gacgaactca gaactagaat ccattcctca acaaaatcaa ggagacgctg tgatcaattt
      301 tggctcaaaa gaaaccattc gtgttcattt tagtccgaac aaacgtcaat taaaattgtt
      361 tgatggagga aaataatgga aaaccaaaat gaaagtttaa tcaagcagta tgttaagcgt
      421 cgtgcaaagc gacgcttatt tttgtggttg tttggtactt ctgcaggttt gattactatt
      481 ttgatcactg tgttcgtgac cctttttcta atactggcgg cgggttcgat tgataattct
      541 gattcagatt caagcagtgg tggcgaagct tttacaggag aatattcgga aggattaccc
      601 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgctgta
      661 ggtgcagcag tgaaatataa attattacca agtgtgatcc tttcgcaata cggttacgaa
      721 tcagcgtttg gtacgtcggc gtccgcaaga aatgatctca actattttgg gattacgtgg
      781 ttcgatggct gtttgtttcc taaaggaact gcacgaggaa ttggtggtat tgaaggtggc
      841 tggtacatga attttcccaa ctcaaaagct gcattctcct attacggctt tatggtagcc
      901 acacaatcaa attttaatgc atgtgtagga aataaaagcc ctggcgcaag cttattgatc
      961 ttaggacgag gtggttatgc cgctgcagga attactgaag atagtccgta ttatacaggt
     1021 tgtatgtcaa ttatcacttc taataaatta accgaatatg atgaattcgc gatgaaacat
     1081 tggggtgaag gtggtaatga taatggcaca attactggtg aatggacgaa tccattccct
     1141 ggcagcagtt tagataaaag tacctttagc ggtggtcaat tatttggaac aaatccaggc
     1201 agtgaattcc gtccgaatgg cttccatgat ggattagatt ttggttcagt tgatcatcca
     1261 ggaagtgaaa ttcacgcggt gcatggagga aaagtcgtgt acgttggcaa tcctggtatt
     1321 tcaggtttag gtgcttgtgt gatcgtgatt aattacgacg ggttgaatat ggtatatcaa
     1381 gaatttgcga atagtacagg aaattctcgt gtgaaggttg gagatcaggt gaaagttggt
     1441 caagtaatcg ggatccgaga tacagcacac ttgcatttag gtttcactcg aatggattgg
     1501 cgtcaagcac aaggacacgc attcacagat gatggtacat ggattgatcc attaccattt
     1561 ttgaatagca gcaaaaaata gatcaggaga aaaaatatgg aaaataaaac tttaaaaatc
     1621 gtcttaacag ctgcaattag cgttagttta atcggcaata tcgcgttgtt ttggcagctg
     1681 aaagaggaac aagcgacaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgag
     1741 aagctcaaaa aaaattcctc ttcagcgcag gcaaagatcg acaagttaaa tcaaactatt
     1801 aatgagaaaa accaagaaat cgatcagcta aagcaacaag atggcacaaa agcaaaagaa
     1861 gaagtaaatg aagcacaaag taagacggca gaagagttcg gtaaattggc cattgataaa
     1921 tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tagcaaccca agaagtgatt
     1981 gacaaattaa gtcctgcaga cgatgatcac caacatgatg attacggaag ttactccttt
     2041 acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga acaaaatttt
     2101 gtcgtatttg ttgattatac aatcggcaat cctcaattca aagatgtcaa accgcaaaaa
     2161 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcacagac
     2221 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag caagacaacg
     2281 tttcaaaaaa cgaaaaagtt atcttagatg gctgggaatc tttatgatta tcggcttttt
     2341 aattcttgct agtccgattt atacgcataa gaaggtagac ataccagatg aggtaagcac
     2401 gaacgcattt ttaaacacga aggaactgac gatggtttca aaagaaaaag gagcgaacca
     2461 gcaattagtt tttgaatttg cgattgacga tgaagatcaa agcttattaa aagaactggc
     2521 taatcttaat tatcgcgtag aagtaaagac agctaaaggg gattatcaag caataaaagc
     2581 caaagtgatt aaggtgtctg atgattattt tgcagtgaag ctatcgcatg taccagaaga
     2641 atatattgct atgcgattaa ctattattcc tgaaaaaatt gatccaaagg tggatatgca
     2701 agaaccgcaa gatctgattt tttatattca cgaagataag gtcaaagatc acgtgaaaga
     2761 cgaagattat gaacaacatg cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa
     2821 agcagcacaa aaacaaattg aatccttcca agcaaccatt gatttgaatg aagaattagt
     2881 gaagaaacta aaagatcaat tgccttatca agtcgcagaa gatcaggaaa atactgaaaa
     2941 taagattaat agttaccagc aagaaattga aacatcgaaa acgcaaatga aagatcaaga
     3001 agagagcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa atatttagaa
     3061 gaataggtgg agtaaaagaa atgaagcgag cgaacttggt cggcaaagat actttcgctg
     3121 agcagaaagt attgattaaa gcctatctca aagttgtaaa agctaaagaa atatgtcaag
     3181 aagtgaatta tctaacacaa caatatctga gttgaagaat ggtagaaaga gtttagatac
     3241 cttaaaattc agatcatttg aagctctata tatagctgct aaaaaatatt ttacagctga
     3301 taataaataa aaaattaagg tgggttctat gtgaaaaaaa cgtatcaaaa gtttttatgg
     3361 attagctata tcgtcctatt tatcgttggg tttctcgtca gtgtgtccgt gatcgtcgga
     3421 acgctttttc ttttgttctc tgacgaagat ctggtactta aggggttaat gagtacgtta
     3481 tcgtgtgtca tgttacttta tttatgtata cagatcaacg aaattatgcc ccctcattgg
     3541 caagaaaaga agaaagaatt taaggaagaa atcgaaataa aatccaatga ctggattgaa
     3601 tacacatggc ttcctctgaa aagaaatagc aaacgaaagg aattttgggc attgcaatta
     3661 tggccaatag gaaaactttt tatttatatg gcttcactta tggttacttt aatcggttat
     3721 ttggtggtgg tctatggggt ggcctattac ttgttaagtg agccaaaaca gatcgcggcg
     3781 tcaaatattt tcacggcagt aatgacatta atgtgtgtca tgtgcttttc tatttttagt
     3841 tacttactcg tccaatttat caggcaaaat ttaacaatga gtaaactttt caaaagttta
     3901 ttggacttac ttatcttagg cagtatgtta acgactctaa cagatcggtt cgaacaatct
     3961 gaaaaaacgg tagaggggat aaagaacttc ttaacgacga ttgatcaatt tataaatgga
     4021 tatatgtatt tgtttgaagt aggctttatt ctcactttgt ttggttgtgt atttataaaa
     4081 ctcattaaaa gatcaatcga aaaagaaatg gttattgatc taattccgac gctgagcggc
     4141 tatgagtatt acgttgattt tggaaagtat gaggagttga tccatacgga tatcgttcac
     4201 acgagtatcc atgaagtaac cgttaagggc atcagatcta ctttaaaaga tggacaggag
     4261 acgaaaacaa tcgaccgaag aaaagaggtc tacaattttt ataaagttga agcaagtcta
     4321 cgatatggca ttgggaaatt gaggttagat gggagtaata ttcaacaaaa caaaccatat
     4381 gttttaggaa aagtggtgat ttagaacttc ctatgaaaga tgaataaata aggagaatct
     4441 tttgaaaatg attcttcttt acagaattgt gcttatagtt ggcacggagt taaaaatctt
     4501 aaagaaggag cagttattca aagtgaaaga acatcataat cagttactat gtattaaaac
     4561 gggtacgatg aacattcaat accgaacaga atatttagaa ttaacgaatc aaactgattt
     4621 tttgtcgatt cgagagcaac ataagcaaga aaaattaatg gctacttata gccaattgat
     4681 tgatgagtgg gaacaattca aggaatctaa aacataaaat tccatttaaa caccttattt
     4741 tttaaagagc ataaggaaac gtgaccccac ttttaaaaaa agtggttgga taggctgtgc
     4801 ctatcacgtt tctgaaaaaa ataaatgacc ccaaaatacc aatatatagg atagtacaag
     4861 gatagcaaag gatagtaaaa ggatagaggt aggatagcga aaaggatagg atccttttca
     4921 ctatcctata ttatattgga ggatatttat gaatgttaaa attagagatt tagatccaaa
     4981 attcatttct gaaattgatc gaagatgcgt tgaactaagt aatcgtacag gaaataaatg
     5041 gagtcgtaat gattatttaa agctactcgt tgaaaatgat tttgatcgtc ctttgatgga
     5101 atacaaacaa gaaaagtttg accagttatt agaaaagttt tcagaaattc aaagttataa
     5161 cacaaagata ttagaagaat atgttagcca aaataatcgt attatagaaa tcttgattga
     5221 gcaaaataga ggaagtgaac tttgatatgg caaaggttat agacaaacac atttactttc
     5281 accaaacaca tatcgatgta ttggatgaaa ttatttctac tcatgcagag gttaaaaact
     5341 atagtgaagc tgttcgcttt ttatgtatga actatgatcc agaagtggag aaaaaagtaa
     5401 attccaatca agcaaagttg aatggaatga gtaaagaaat ttctatttta accgaaatgg
     5461 tttcagaatt tgcggatctt catttgaaag atacaggaat attaattggt aaaaattcaa
     5521 ctgtttatca agaagcaaaa gaacgagtgg aaggaaaaat taaatccaat caaacaaaaa
     5581 aatattcccc tcagttgaag aaacctgtga atgaatctgt tcatgccaga gtagatccat
     5641 ttgatccaac gaagattcta aaaagataaa aatgaaggag gataataatg actcaatcgc
     5701 cttcagttat tctcacttgt caatttactt tgccgaataa aaagtcattt tctacttata
     5761 ttgactatat tacaagggaa caggctcttg aagaaaagga acgacgtacg cccgaagaag
     5821 aacaggaact tcaacgaatc aagaatgctt tagaggagtt ttatattcct gaaggagaaa
     5881 ctttttcgga gagtaagacg ggtaaagaat tcagtgaaac gtcgaatgaa gctcaatcgt
     5941 tgatggaaca tggaatcaat tttgacaagt tagaggaaca agattttacc aattatcttt
     6001 cttatatgac acgtcactat gcgttagaac aaaagaaaga actgacgcaa agtgaacaaa
     6061 aagaatacca acgcttgaac caagcaatga aaaaatatca aacagaaccc cataaaaaag
     6121 cttcaggaaa tcttcctgga gttttttcta tagcttcaga cgaagtgaaa ggaagtgatc
     6181 tgaaggagat taagcaaatc ttccaaagag gacaacaaaa aggctcaatc atttatcaag
     6241 atgttgtttc tcatgataat tcgtatcttg aaaaaatagg attgtacgat cccaaaacag
     6301 atactttaga tgaagaaggg ttaaaagcag ctggaagaaa aatgatggag acattattcg
     6361 agaaagagca actgaacgaa acaggctact ggatggcaac gattcaccgt aacactaaac
     6421 atatccatat tcattttgca gttgttgaaa aagaaaatac gcgtaaacct tacacggagg
     6481 ttgaaaatgg tgtgagttat atcgtaccga aaggtaagag aagacaagca acattagacg
     6541 caatgaagac caattatgta catgagttag aacgtttttc ttatgaaaag gatcgaatga
     6601 ttggtatgga gaaacgaaat ttattgacgc ggaaaagtga tttgcgtaac accttaacaa
     6661 aagtggtaaa agagccagcg cgttacgatc gacacgcgat gaacttatta aatgaagtat
     6721 ataaaaccct acctgacaaa cgttcggaat ggaattacgg ggatgaaaaa cgaaccaaac
     6781 tttcaccata cacaagagag aaactcgatg agttaaccaa atatgtttta acaaaagaac
     6841 ctgaatatca agaatatata cagatttcaa aagcattgaa agaagaagca aaaagtcttt
     6901 atggggaaag caagagagaa tcaaaggatg cagagaaaaa tgctctcttt gatttaaaaa
     6961 agcgcacagg aaatgccatc ctaacagagc tgaagagaca ggatagtcaa ataaaaccat
     7021 taatagaaag tgttgatcag atgcaatcta tttacaatga acgatccttt cgaacgctta
     7081 aacaattgcc aacgaatgaa tacaaatata gttacgaaga attcatgaag cgcaggaaac
     7141 aattccaaca aagagttgtc acgcagcgga gtttgcgtca attaaatcga acgattaatg
     7201 cccatgaaga aaaataccac gcaagtaaag catacgaaga gcaacaacga agaattcaaa
     7261 tggaacaaga aagacagtaa agacagtagg aggaatagca atgaaacaat atttagatca
     7321 atggaaagtt atagaaggat cattaagaga agaacgaatc gaacaacttc ctgattgttt
     7381 agaaaaagag catttgttcc agataagaga aatgttgaga aacgaacagt ttgatccaaa
     7441 tcaattttta gtcgttgagt acccagctac tggtgtgtat tgttgtaatc atgtgaatgg
     7501 tgaaaaatat tttatcattc aagaatatga gggcaagtta gcaccttatt atactacttg
     7561 ggaaatgaat gaagagggaa tcaataactt tccatgtaaa agcattgaag agtccatttc
     7621 actcacagag tgttagttgt taagtgaata aaagaagtaa aagagtcctg aacgtgattg
     7681 tccaggactc tttttttcaa tgaattgttt tccgccaact cattgagtgg tgcgatttgc
     7741 gactacatta tatcaaaaga aagagaaggg aggaagcaga aatggttcat tcaacgattg
     7801 gagttgttgt tgaaaaaagc agagacaatc tcgtttttgt gacagaaatt caaacaggca
     7861 gatcttttgt cgtcacagac aaagcggcaa aagcttatca aagtagcgat attttaacgt
     7921 taaatctgac tacgaagacg ttcgtcgatg cgacagaaga ttatccattt gtttagtcta
     7981 atggtgataa gtcaagatga aatttaagat tt
//
LOCUS       DACEOM010000109         7804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEOM010000109 DACEOM010000000
VERSION     DACEOM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.114"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            213..1940
                     /locus_tag="IW781_002715"
     CDS             213..1940
                     /locus_tag="IW781_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1183684.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            2012..2305
                     /locus_tag="IW781_002716"
     CDS             2012..2305
                     /locus_tag="IW781_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1183685.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(2397..3257)
                     /locus_tag="IW781_002717"
     CDS             complement(2397..3257)
                     /locus_tag="IW781_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1183686.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            3349..3777
                     /locus_tag="IW781_002718"
     CDS             3349..3777
                     /locus_tag="IW781_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1183687.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            3939..4115
                     /locus_tag="IW781_002719"
     CDS             3939..4115
                     /locus_tag="IW781_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1183688.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            4142..4588
                     /locus_tag="IW781_002720"
     CDS             4142..4588
                     /locus_tag="IW781_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1183689.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(4742..6121)
                     /gene="brnQ"
                     /locus_tag="IW781_002721"
     CDS             complement(4742..6121)
                     /gene="brnQ"
                     /locus_tag="IW781_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1183690.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            6827..7798
                     /locus_tag="IW781_002722"
     CDS             6827..7798
                     /locus_tag="IW781_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1183691.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
BASE COUNT         2637 a         1394 c         1601 g         2172 t
ORIGIN      
        1 tttaaaaaga gtagaagaac gggttcaatt gttcttttac tctttttttt cgcgtgaaat
       61 ttactgaatc tgatttatgc gaacacgtcc aacacctttt gataaaagaa tcctttcttg
      121 atttaagcgt tttttggtcc tcatactttc atatctaata agtgaatgct atacttatta
      181 agaaccatat ttcagaaaga ggtgttattt tcatgtcttg ggaacaagtg tatgaacaat
      241 gggtacatga agaaaattta gaagaaaact taaaaaaaca attaaatgat ctgggtgaag
      301 atcctgaaaa acttgaagat gctttttatg caccgttgga attcggtact gcaggtatgc
      361 ggggaatctt aggacctggg atcaatcgaa tgaacatcta tacggtaaga caagcaacag
      421 aaggactagc acgtttcatg gatacacaag gtgaagaaac aaaacgccga ggtgtcgcta
      481 tcgcttatga ctcacgccat cagtcaccag aatttgcaat ggaagcagcc aaaacactag
      541 cacatcacaa tatcccgtct tatgtattcg aaagtctgcg tccaacacca gagctttctt
      601 ttgcagttcg tcatttgaag acgtttgcgg gaatcatggt aaccgcatct cacaacccag
      661 cggcttacaa tggatataaa gtctacggag aagatggcgg acaaatgcct cctgcagacg
      721 cagatgcatt gacgaaatat gtccgtgaag tcagcaatcc gctaaaagtc gaagtattat
      781 ctgacgaaga agctaaacat agcgggttga tcacgatcat cggagaagaa gtggacgctg
      841 cttatctaga agaaatcaaa gcagtgacga ttgatcgtga attggttgaa acaatgggga
      901 aagatttgaa acttgtgtac acgccacttc atggaacagg gaaaatgttg ggcgaacgtg
      961 cgttgaaaca agctggattc gaacaattcg tactagttcc cgaacaagct gtggcagatc
     1021 cagatttcac tacggtcaaa tcgccaaatc cagaagaaca ttcagctttt gaatatgcta
     1081 tacgacttgg agaaaaagaa ggagctgact tgctgattgc aacagatcct gatgcagacc
     1141 gtttaggcgc agcggttcgt ttgccagatg gttcttatga agtattgact gggaaccaaa
     1201 tcggagcatt gtttgctcaa tatattttgg aagcacatca atcaagaggc actttaccag
     1261 aaaacgcagc ggtattgaaa tcaatcgttt ccagcgaatt gcctacagca attgcggaac
     1321 actacggtgc taccatgttc aatgtgttga cagggtttaa attcattgct gaaaaaatcc
     1381 aacaatttga agaagaccat tcacatactt ttatgtttgg attcgaagaa agttatggtt
     1441 atttagttaa accttttgtt cgcgataaag acgcgatcca agcattgctt ttattagcag
     1501 aagtagcagc ttactataaa aaacaaggca aaacgttgta tgacggcttg caaacgattt
     1561 ttgaaaccta tggctatttc gcagaaaaaa cgatttctgt gacaatgagc ggacaagaag
     1621 gttctgcgaa aatctctgct ttgatggaaa aattccgtaa agaagcaccg acagaatttg
     1681 ccggagcaaa agttattcaa acagaagact ttaaagaatt gacgagaaca gaccgtgaag
     1741 gaaacgtaga aaaactaaca acaccgccat ccgacgtatt gaagtataca ttggaagaaa
     1801 atggctggat cgctatccgc ccttcaggaa ctgagccgaa aatcaagttt tatattggtg
     1861 taaaaggaag ctctacagaa gacgcagccg aaaaagtagc ctcttatgaa gcgattatca
     1921 aaaagatgac agaagaataa aaaacttgag actgatggaa aaagtggtaa actttagaag
     1981 tcagtctttt ttcaagttgg aggtgaatgg attgccagac acgcaaaaat caaaagtaga
     2041 gaaagtcagt caattattca aaatgttgag tgatcccact cgtcttaaaa ttctgctata
     2101 tctgaaagac ggggaacaaa atgtgacagc aatcacccaa gcagtggaaa tggagcagtc
     2161 tgctgtatcg catcaactac gtttattaag agaaaatcat gtcgtcaagt ctcatcgcga
     2221 gggaaaagcg attttgtata gtcttgatga ttctcatgta ctggatattt tgaaccagac
     2281 actcaaacat gtggaacatc aataacgata ggtgaaaata gataaacact ggagtgtgac
     2341 aacgaagaaa tttcgttgtc acactccagt gttttttaag aaaaagacaa aaatgattaa
     2401 tcgctttttt ctgttgtttc agttccataa taactatgat cttctaggtc taaagcattc
     2461 aaaataagcg aagccgtttc ttcaatagat aaagatgcca cattgatcac tacacatcct
     2521 agtttttcat aaagatcatt cgcaaaatcg agttcagctc taattttttc gatatcggaa
     2581 taagcagtat cagggtttaa tccgtaagag cgcatccgtt ctttacggat attatttaaa
     2641 atatctgggt tattcgttag tcccacgatt tttttaggat cgatttccca cagctgtttg
     2701 ggaatatgtg cttggggaat caaaggtaaa ttagcaactt ttaaattttt atttgctaaa
     2761 aacaagctca aaggcgtttt ggaagttcgg gatacgccta gcaaaacgac atctgcttct
     2821 aaaaaaccac gaggatcttt cccatcatcg tattttacag caaattccat tgctttgatc
     2881 cgtttgaaat agttctcatt caataaatgg agggcaccag gttgccgaat tggcttgata
     2941 ccagttaaac gttcaatttc tgatatcggt ggcgtcatca catcgaaact aaataattga
     3001 tgttcttcac aaaaagaacg tgttatctcg atcagttctt gattgattaa tgtatgcagg
     3061 acaagtgctt tttcttctaa tgcatcttgc aatgcctgcc ttaatgtttt ttcatctgtc
     3121 acgaacgttc tgcggaatag gttgaactca acggtaggat actgagccat agtagcttgc
     3181 gctagttttg tcgcagtttc tccagcagag tcggagatga caaaaaaagt aacgatttct
     3241 gtagaatcgg aagacatgga taaaacctca cttgcatttt ttttatgttt attataccat
     3301 actctagaat gcgtacttcg taatcatttt gatttggagg aataagtcat gaaaaaacag
     3361 acattgatcg aacaatcatt agaaacattg aaaaataacg gttttaaata tactaaaaaa
     3421 agagaagcat tgttaactta tttagtgaag cgtaaccgct acgtatctgc taaagaagtt
     3481 tttgactata tgaatcagga attcaaaggg gtaagttatg atacgattta ccggaatctc
     3541 catgattttg cagagatcga tttactggaa gaaaccgatt taaatggtga aatgaagttt
     3601 cgttttcatt gctgtcaggg ggaaatcggc catcaccatc attttatctg tacagtgtgc
     3661 ggaacaacga aagaaatcca tatgtgtcca atgaattatt tcgaagaaca gctaggtgga
     3721 tgcacgatcg aagggcatag attcgagatt ttcggaagat gtgaaaattg tcaataagga
     3781 tttaaataaa ataacatttg ataatttaaa aaaaaagcaa gatttccttg acggtcaaaa
     3841 cagcattaga tataatattt acaggacagt cttgtttttt gagatatatt tcaataacaa
     3901 ttacggttac tttttaagct cggagggagg gaattaacat gtcaaaaaca gtcgttcgca
     3961 aaaacgaatc acttgacgat gctcttcgtc gcttcaaacg ttccgtgtca aaagcgggta
     4021 ctttacaaga atctcgtaaa cgtgaattct acgaaaaacc aagtgtaaga cgcaagaaaa
     4081 aatctgaagc agctagaaaa cgcaaaaaat tctagttctg tgatgaattg gagttggtag
     4141 tgtgtcacta ctaagtagat taaacgatga catgaaagca gcgatgaaag cgaaagataa
     4201 ggaatcttta caagtcatcc gaatgatcaa gtcatctata caaaacgagc agattaaaga
     4261 aggtcacgac ttgacagaag aagaagaact aacggtgttg tctcgcgaaa tgaaacagcg
     4321 acgtgattct ttgcatgagt ttgaagaagc aggacgtgac gatttagctg aaaaagtaaa
     4381 aagtgaaatt gtaatagtcg aaaaatatat gccggaacaa ctttccgacg aagaaattcg
     4441 acaacttgtt caagaagcaa tcactcaaac tggtgcttca tcaatgaaag aatttggcaa
     4501 agttatgggt gcaattatgc ctaaagtcaa aggaaaagca gatggaaacc aagtgaatgc
     4561 gattgtgaaa gaactattac aatcttaatt ttatcattta aactgcggac agtgattgtc
     4621 cgcagttttt tgtgttcgta aaaaaagaac taaaaaacag ctagaagaaa gaataagatt
     4681 ctttcttcta gctgtttttt tcctgtttaa tatctcttat gttaaattgt gtttgatttg
     4741 gttattcttc tggtagttca tttgcttctt gtttataccc tgataggcga taggcgtaat
     4801 aaaagagtaa gctgccagcg taaccaagta acgttgggac catccagcca agtcctaatg
     4861 cagcaaaagg aacgtgatga tgataaaatc ctagtaagct tgctacgaca gtttgctgac
     4921 tcactaatgc aggcgaattt gccaacatat ccaatacggc tggaacggca gaaaagaaca
     4981 tcgttacttg atagacaaat ggactctttt ggaagaattt tgctgttaag gaaagcaaga
     5041 ttaacgcaag tgacaatgga tacaaaagca tcaatactgg taaagaccat tggatgatat
     5101 tatccaatcc ggcattcgca acgacaaaag agacaaaagt cgttaaccgc agccatgtca
     5161 tataactgac tttagggaac agtttatgga aatcttgtgc aaaagaaaca acgagtccca
     5221 tagcggtagt gaagacacct aatgtgacca ttacccctaa gaagctagca ccgaagttac
     5281 ctaaataaaa attagtgatc tgagaaagtg cagttccgcc attttctgat aaaggcatct
     5341 tattcaaact catcgcaccg agaaggacca gaccaaaata aatcatgact tctaacgcaa
     5401 cgctcaggct accagctttt gctgttaatt ctgtgacttt tttatcttta aaaccaagat
     5461 cacgaactgc atggacaaaa gtgacactta aagcaagtaa agctactgca tcaaccgtgt
     5521 tgtatccttc taaaacgcca tttgtcaaag cgttcgtctg ataagaatct gcagcagatt
     5581 ggtttagatt ccccatgggt tggaataaag aaacgacaaa aatgatagct aataatgcaa
     5641 gaaacaagga gtttaaatac tttccgacat atttgaccaa acgtgttgga tgtacagtaa
     5701 ggaaataagc aagtaaaaag aaaagagcag agaataaaaa catacctaat gaatcatatt
     5761 tttcaggcaa aaagggttta gcagccattt caaaagcagt tgctgctgtc cgcggtgtac
     5821 caaagaatgg tccgatcgtt aaatgaacaa ggatcaagaa caaggcagca taccattttc
     5881 cgacaggttt agctaagtca tacagaccat cactttttgt cactactaca gctaagatag
     5941 ctaataatgg gaaaagagcg cctgagatgg caaatcctac accagcgctg atccagtttg
     6001 atccagccat ttgacctaag tggacaggaa agatcagatt acctgacccg aagaacatcc
     6061 cgaagataag tgaactgacc atcaataatt gcttccatga ataagttttt gtttcttcca
     6121 taaatttttc ctccaataat tatcattttg agtaacaaaa taaacgttgc agaaactaga
     6181 aatgccacta atatcagatt ccatgaaaac caccacctta aaatataaaa aaaagccctt
     6241 ttccacttgg gaaaagggct ctagattcta gaacggtacc accttttttc gcttaaaaaa
     6301 gcttcttcat tcacggacgc ttttcgataa agttatccgc tagcctgata atggaggcag
     6361 ccacaatgat ctacgttttc gacattgact cggaaatgat gttcgtctat tcgatttatt
     6421 gtcatttctc acttacatga ctcactggga ataaatcgct gaatagccta ctgttttcct
     6481 cgacgctttg ctgtatattt atttgttgac gttataatac acctgttttt tttgaaatgc
     6541 aagaatattt caaatatttt gttttctttt tttgaaacaa gaaaaatacc cagagaatac
     6601 ttgctaagtt agacggaata cttgtcctct atcttatgca tagcttacat gatcagcttt
     6661 ttttattcaa acgagacctt tagagaatgg aagcagctat tttttaacgt ttcttggtac
     6721 ttatggttaa gcaactcttt tagaaccttt tcttatttgc tatatagttt cttctgagta
     6781 tggtaaaata ggaaagaatg aatcgaaaaa aaggggatgt ctatttttga cggaagagtc
     6841 tagttcttta gagattagat tgacggctgc tgatgatgtc agcatgcttt taggtgcaca
     6901 cgacaagcat ataaaactga ttgaagaaac aacagaaaca accatccata cacgtggaga
     6961 aatcattcag atcattggcg aacaatccca gatttccttg gctgcctcag tcattcggac
     7021 attgcaggaa ttgatcaaac gaggaatcca tgtaagtaca ccagatgtcg tgacggcact
     7081 gaaaatgggg caaaaaggca cattagatta ttttgttgaa atgtatgagg aagagatcgt
     7141 caaagaccgt aatggaaaac caatacgggt aaaaaacagc ggccaaaaga aatatgtcga
     7201 atcggtagcc aaacatgata tcgtatttgg cgtaggacca gccgggacag gaaaaacttt
     7261 tcttgcagta gtattagcaa tcgctgcctt gaaaaaaggt gaagtgcaaa aaatcatttt
     7321 gacgcggcct gctgtggaag caggggaaaa tctaggcttt cttcccgggg atctaaaaga
     7381 aaaagtagat ccttatctgc gtcctgttta tgatgcttta taccaaattt ttggattaga
     7441 tcatacgaac cgattgatgg aacgaggcgt gatcgaaatt gctcctttag cttacatgcg
     7501 ggggcggaca ttggatgatg cattcgttat tttggatgaa gcacagaaca cgacagttgc
     7561 tcagatgaaa atgttcctga cacgtcttgg ttatcaatca aaaatgattg tcaatggaga
     7621 tacgagtcag attgatctgc ctcgagggac aacaagtggt cttgttcatg cagaacgaac
     7681 attaaagcaa atcaagaaaa tcgattttgt taattttgaa gcaagtgacg ttgttcggca
     7741 ccctgtagtt gccgaaatca tcaaagctta tgaaaaggca gatctccatc aagaataagc
     7801 tttg
//
LOCUS       DACEOM010000110         7761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEOM010000110 DACEOM010000000
VERSION     DACEOM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.115"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1094
                     /locus_tag="IW781_002723"
     CDS             147..1094
                     /locus_tag="IW781_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1183692.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1106..2026
                     /locus_tag="IW781_002724"
     CDS             1106..2026
                     /locus_tag="IW781_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1183693.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2067..3524
                     /locus_tag="IW781_002725"
     CDS             2067..3524
                     /locus_tag="IW781_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1183694.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3869..4483
                     /locus_tag="IW781_002726"
     CDS             3869..4483
                     /locus_tag="IW781_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1183695.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4476..6200
                     /locus_tag="IW781_002727"
     CDS             4476..6200
                     /locus_tag="IW781_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1183696.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6197..7648
                     /locus_tag="IW781_002728"
     CDS             6197..7648
                     /locus_tag="IW781_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1183697.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2618 a         1291 c         1543 g         2309 t
ORIGIN      
        1 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
       61 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
      121 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
      181 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
      241 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
      301 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
      361 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
      421 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      481 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      541 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      601 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      661 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      721 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      781 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      841 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      901 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      961 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
     1021 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
     1081 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
     1141 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
     1201 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
     1261 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
     1321 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1381 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1441 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
     1501 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1561 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1621 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1681 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1741 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1801 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1861 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1921 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1981 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     2041 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     2101 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     2161 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     2221 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     2281 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     2341 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2401 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2461 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2521 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2581 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2641 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2701 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2761 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2821 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2881 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2941 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     3001 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     3061 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     3121 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     3181 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     3241 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     3301 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3361 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3421 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3481 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3541 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3601 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3661 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3721 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3781 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3841 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3901 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3961 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     4021 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     4081 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     4141 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
     4201 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
     4261 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
     4321 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
     4381 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
     4441 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
     4501 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
     4561 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
     4621 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
     4681 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
     4741 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
     4801 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
     4861 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
     4921 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
     4981 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
     5041 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
     5101 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
     5161 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
     5221 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
     5281 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
     5341 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
     5401 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
     5461 agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca
     5521 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
     5581 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
     5641 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
     5701 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
     5761 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
     5821 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
     5881 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
     5941 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
     6001 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
     6061 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
     6121 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
     6181 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
     6241 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
     6301 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
     6361 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
     6421 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
     6481 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
     6541 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
     6601 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
     6661 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
     6721 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
     6781 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
     6841 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
     6901 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
     6961 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
     7021 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
     7081 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
     7141 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
     7201 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
     7261 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
     7321 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
     7381 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
     7441 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
     7501 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
     7561 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
     7621 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
     7681 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
     7741 tcatgacctc tcgataaacg g
//
LOCUS       DACEOM010000111         7700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEOM010000111 DACEOM010000000
VERSION     DACEOM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.116"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            544..1107
                     /locus_tag="IW781_002729"
     CDS             544..1107
                     /locus_tag="IW781_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1183698.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IW781_002730"
     CDS             complement(1386..1673)
                     /locus_tag="IW781_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1183699.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IW781_002731"
     CDS             complement(1663..1992)
                     /locus_tag="IW781_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1183700.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IW781_002732"
     CDS             2297..2626
                     /locus_tag="IW781_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183701.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW781_002733"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW781_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1183702.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="IW781_002734"
     CDS             complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="IW781_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1183703.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="IW781_002735"
     CDS             complement(4890..5498)
                     /locus_tag="IW781_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1183704.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IW781_002736"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IW781_002736"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1183705.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="IW781_002737"
     CDS             complement(6528..7496)
                     /locus_tag="IW781_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1183706.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2353 a         1532 c         1280 g         2535 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tagttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatta
//
LOCUS       DACEOM010000112         7619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEOM010000112 DACEOM010000000
VERSION     DACEOM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.117"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(102..626)
                     /locus_tag="IW781_002738"
     CDS             complement(102..626)
                     /locus_tag="IW781_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183707.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="IW781_002739"
     CDS             complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="IW781_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1183708.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1215..2195)
                     /gene="pta"
                     /locus_tag="IW781_002740"
     CDS             complement(1215..2195)
                     /gene="pta"
                     /locus_tag="IW781_002740"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1183709.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2348..3028)
                     /locus_tag="IW781_002741"
     CDS             complement(2348..3028)
                     /locus_tag="IW781_002741"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1183710.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3154..4023
                     /locus_tag="IW781_002742"
     CDS             3154..4023
                     /locus_tag="IW781_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1183711.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4049..4564
                     /locus_tag="IW781_002743"
     CDS             4049..4564
                     /locus_tag="IW781_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183712.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4611..5174)
                     /locus_tag="IW781_002744"
     CDS             complement(4611..5174)
                     /locus_tag="IW781_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183713.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5229..5408)
                     /locus_tag="IW781_002745"
     CDS             complement(5229..5408)
                     /locus_tag="IW781_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183714.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5485..5994)
                     /locus_tag="IW781_002746"
     CDS             complement(5485..5994)
                     /locus_tag="IW781_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183715.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6090..6470)
                     /locus_tag="IW781_002747"
     CDS             complement(6090..6470)
                     /locus_tag="IW781_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1183716.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6800..7549
                     /locus_tag="IW781_002748"
     CDS             6800..7549
                     /locus_tag="IW781_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1183717.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2167 a         1580 c         1354 g         2518 t
ORIGIN      
        1 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
       61 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
      121 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
      181 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
      241 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
      301 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
      361 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
      421 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
      481 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
      541 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
      601 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
      661 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
      721 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
      781 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
      841 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
      901 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
      961 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     1021 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     1081 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     1141 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag
     1201 ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat
     1261 acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga
     1321 attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct
     1381 gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct
     1441 ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg
     1501 tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc
     1561 gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac
     1621 aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc
     1681 acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc
     1741 atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga
     1801 acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac
     1861 gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat
     1921 gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta
     1981 tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt
     2041 atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca
     2101 gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga
     2161 acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata
     2221 tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa
     2281 aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc
     2341 tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga
     2401 gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg
     2461 gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt
     2521 cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt
     2581 caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt
     2641 cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat
     2701 ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag atatttctta
     2761 acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt
     2821 ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt
     2881 ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt
     2941 ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt
     3001 gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca
     3061 cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact
     3121 aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg
     3181 gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt
     3241 tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct
     3301 cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag
     3361 gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc
     3421 tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt
     3481 ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat
     3541 ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg
     3601 cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta
     3661 aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt
     3721 tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat
     3781 aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta
     3841 gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga
     3901 gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg
     3961 gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg
     4021 tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct
     4081 tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt
     4141 tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt
     4201 tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt
     4261 cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg
     4321 tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt
     4381 caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt
     4441 ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta
     4501 ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag
     4561 ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc
     4621 ttttgcatat gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca
     4681 tggcgttcgt ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg
     4741 gagcgattag ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct
     4801 tgtcctctaa aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt
     4861 tctttatcaa tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc
     4921 agtggctgat cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact
     4981 ttaccgtcta tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg
     5041 attgcttctt ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt
     5101 tccatatctt ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc
     5161 gcaaaacgaa tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg
     5221 caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg
     5281 attcttgccg cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt
     5341 tcgactacat tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt
     5401 ttttccatgt tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta
     5461 aaaaagagaa gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc
     5521 atctattaat cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt
     5581 cacttttgct tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt
     5641 gaactcttcc gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg
     5701 tacggttgtt ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg
     5761 gtattcatat gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc
     5821 agatatgtta tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat
     5881 ccgatattcc tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat
     5941 accggtaatc cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca
     6001 ttctcctgtt tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa
     6061 acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct
     6121 ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat cttctctaat cggttctgat
     6181 tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca
     6241 agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt agcaattcca
     6301 aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc tcgttctttg
     6361 cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc acgtgatttc
     6421 tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat atgagcactc
     6481 ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa atgtgtcaga
     6541 ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag
     6601 gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa attatatttt
     6661 caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa gactagtttt
     6721 ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact acatttagtg
     6781 tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc agctaaaagg
     6841 ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg tcatgactct
     6901 tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca cgcttggtgt
     6961 attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag gtattttgtc
     7021 tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta tcggtgaaca
     7081 gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat ggaatcataa
     7141 tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa tcatcagtac
     7201 tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg atcctcggcc
     7261 gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca gtttcttgcg
     7321 ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg tcctttggta
     7381 tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt
     7441 gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag aacgtgaacg
     7501 tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa aaaactgagc
     7561 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaa
//
LOCUS       DACEOM010000113         7577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEOM010000113 DACEOM010000000
VERSION     DACEOM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.118"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(595..1941)
                     /gene="gor"
                     /locus_tag="IW781_002749"
     CDS             complement(595..1941)
                     /gene="gor"
                     /locus_tag="IW781_002749"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1183718.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2075..3316)
                     /locus_tag="IW781_002750"
     CDS             complement(2075..3316)
                     /locus_tag="IW781_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1183719.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3334..4257)
                     /locus_tag="IW781_002751"
     CDS             complement(3334..4257)
                     /locus_tag="IW781_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1183720.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4478..6970)
                     /locus_tag="IW781_002752"
     CDS             complement(4478..6970)
                     /locus_tag="IW781_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1183721.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6977..7444)
                     /locus_tag="IW781_002753"
     CDS             complement(6977..7444)
                     /locus_tag="IW781_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1183722.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2087 a         1646 c         1218 g         2626 t
ORIGIN      
        1 taccgagaaa ctggcttttg aacaccgttt atcagaggtt gtaaaatcag tgttttgtcc
       61 tgagtattag agaaacaaat ggaaaaatag ttcctcatat tttgccgttt tttgagctaa
      121 taccgaagga cagctgattg aacactattt gttcggaaaa agtgactaac tatgacacaa
      181 tttttttcac agtccctttt ttcacttgat ttgattcgta ccagcgaact tgacgagaac
      241 tttcttggtt tggtaactat atggtattct tttaacatcc tcgtttgctt cttctaattt
      301 atttatacaa agtcacactt ttttaaacat ctgttcttcg atacatttct atttctttta
      361 aacatcgata ccaatatttt gaacttaaga gtgcatacca aatggattca tttactcttc
      421 gtcagttacg aagataatca aaatctccaa cttccctact tatttcgttt tgagaattat
      481 cgaagcaatc ttacataaat aactaaaaaa caaaaaacca gaaaatcagt atactgattt
      541 tctggtttta catggtttgt tcaataaaaa gctgttggaa ctgttaatat taaattatcg
      601 catcgttacg aattcttccg ctcctgttgg atggatggct actgtattgt caaagtcttc
      661 tttagttgca cccatcttga tagccacggc aaaaccttgc agcatctcat cgacaccaac
      721 accaatagca tgtaagccga cgattctttc ttcttttccg acacagatca atttcatttc
      781 gcatttttgg cgataatcat ttaatgcaaa gtacattggg gtaaagcgtg aacgataaac
      841 tttcaattca ttttcgccat atttttccaa tgcttcttct tctgtcaaac caattgttgc
      901 aattggtgga tgcgtaaaga cgactgtcgg aacaagttta tagtcaagat aagaatcttt
      961 ctttccatta aataaacgtt ctgataaacg tctaccagct gcgattgcaa caggagtcag
     1021 atctaattta ccgatcacat caccaacagc aaaaatatgt tcatccgttg tattttggta
     1081 tttatctact ttgataaacc cttttttgtc caattccaca tttgtatttt ccagacctaa
     1141 tttttcggta ttcggtacac gtccaccggc aaaaagtaca caatcaccag ttaaggtttc
     1201 accattttca aatagcaccg tatattcgtc attgttttta gtaatttctt tagcaatatg
     1261 atgtgcgtaa gtttttattc cagcttcttg gtacatttgg atcaaattgt ccgaaagcat
     1321 tttatcaaac gttctaagtg gtcgttcttt tcgatatgcc cacatgactt cactacctaa
     1381 accacgaaat acacctgaaa gttcagccgc aatgtatcca gcacctaaaa cgattgctct
     1441 cttaggtaac tctgttaatt caaaaaatcc atttgaatca attgcgtatt ctccaccagg
     1501 aatggtcatt ttacttgcac ggcctccagt agcgatcaag attttaggag cacgataggt
     1561 ttcaccgttt acttcgatcg tttgactatc gataaaagta gcgtaacctt ttatagcctc
     1621 gacatgattg ctatctaatc ctctttgata agcaccgtgt agaaaatcaa tatatttttc
     1681 tcgtctttct actagaccgg cgaaatcaac attttttaaa tctgcttgga taccgtaact
     1741 tgaggcatct cgttcaattg tttccaaaat cgtacttgct tgccacataa cttttttagg
     1801 tacacaaccg acgttgacac atgttccgcc caattcattc gcttcaatca gcaaaacttt
     1861 tgcgccatgc atccctgctc tgtttgctga agcaataccg ccagagccgc caccaattac
     1921 gatgtaatca tatatttgca ttttgttcct ccttattgaa gagatcaatc aaaaccttta
     1981 tttaacgatc ctttttctac agaaaacaag tgtaacactt ttcttgaatt ttgtatgcga
     2041 agatgcttaa aacagggata tatcagcttt ttccttattt acgttttcgg atcatatctc
     2101 caggtttcac cggctgtgcg actggcatag ttaaaatcat cattggattt ggtgctcggt
     2161 caatggattc gccttcttca ttatgcataa cttcaacggt ttgactgaaa tgactgaatc
     2221 ctggaccata aaattcgatt tcatctccta cagaaaacaa gtttctctga cgtatcgtag
     2281 caatctgtgt tgcctcatca taagataata cttcaccgac aaatttatat actgggattt
     2341 tacgacgttc accgaataat tgttcgtttt cagttggtgt attgtagtaa aaacctgtag
     2401 ctaattctcg ttgtgctact ttccataact cgtcgatcca ttcttgcttg cagacatagt
     2461 tttcaggatc ttccatataa ctgtctacag ccgctttata gacatttgac accgtagata
     2521 cataatggat cgatttcatt cttccttcga tcttgaagct gtctacgccg ttttcaatca
     2581 attctggaat atgttcgatc attgacatat ccactgcact catcgagaat tcttcttcca
     2641 cttcgcctgt atcagttaaa ctgtttcgtt cttgaccaaa aggcatatcg aacaatccat
     2701 acttccagcg acaagattga gaacatccgc cacggtttgc atcccgcatc gacatatggt
     2761 tggacaatgt gcatcgacca gaataagaga tacacatggc accatggata aatgcttcga
     2821 tttcggtatc cgtattttta cgaatctctg ccacttcctc cattgaaact tcacgagcta
     2881 aaacgacacg ttccaaccct tcttctttcc agaattccaa tgtctcaaaa ttagttgctg
     2941 aagcttgagt ggataaatgg atcggcagac ctggggcttc tgtcgcacaa atttcgatca
     3001 aagctggatc agaaacaatc actgcagaaa taccgacatc acgcaaactg cggaaaaatt
     3061 cccctgctcc atcttcattt ccttcatggg tgaccatatt agcagcaacg tataccttcg
     3121 catcatgttc ttttgcaaaa gcaactcctt cagccatttc ttcataagta aaattacctg
     3181 cacggcttct taaaccatat gcattcccgc caatatatac tgcatctgct ccataatgga
     3241 tcgcggtttt taacttttcc agtgttcctg ctggcgctag gatttctggt cgtttcaatt
     3301 ttctttttac tgtcatttct ttcatcatac tccttatttg atagcagaag gatcaatata
     3361 aaagaatcct gtatctaatt cacggttttc aggatgcaat ttactgattt cgctagtcat
     3421 tttttccgct ttttcttgat cccagctacc ttctaaaatt tgtttttttg cttcggcgaa
     3481 tagtcgagtg atgtttacga atttttcgcc tggagtaaat aatccgtcca atttccatgt
     3541 acagtaatga tgctgataaa gttcattcaa ttcattcgag agattcagat cattgctggc
     3601 aaaaatatgt gtgccatgac tatcttcata aatagaataa tgtgtctctt cttttttagg
     3661 ttccgagata aaaagacctc tttcacgatc tttttgttcg tcttgtttcg tatagttata
     3721 gtaattctgt agcagtggac gtttggattg atggatgcat gtagcaccat acacaagggt
     3781 ttctacagga atatcgagtt tttcttccat cgccttcatc tcttcaaaag gaacttcacg
     3841 agcaagtact gctccaactg cgcctttttt cgcccagaaa ttgatttgac gtgcgctcgt
     3901 tactaatgtt tcagcatcat aaatatacgg tatacgcaat tcaggatttt ttttcatcgt
     3961 ataaataata cctgtatcac caactgtgat cacatctacg tttatttctt ttaaaaaagc
     4021 caaatattca ggtacatttt tcattttttc aggatgaatg atcccattta cagcaatatt
     4081 cgctttttta ccatacttgt gtgctaattc taccaattgt cgctgttctt ctcgtgtaaa
     4141 tgaagcaggc agacgcaatc caaattgctc ttcaccgaaa tatagtgtat ctgcacctgc
     4201 agcaagcaac tgttctgctt gttcaatagt ttctactgtg gttatgagtt cgatcataat
     4261 tttctcctcc tacaacttca taatagtagc atagctctaa aaactttcca atacttcttc
     4321 tatttaaagt cggatttttg aaaattattt cacaaaaaat caaaataaag ttcgatatat
     4381 tctgtccaaa tataaaaaga gattgtgaca aaaatcttgt cacaacctct taatcaagtc
     4441 ttactattat aaaaattcag ctacacaggc caagtaacta tagggaacct actgtttcct
     4501 ttttaggtcc gcgaacattc aatgtgatct taccttttga agcacctact gttacgtggt
     4561 cacctaaacg aatttctccg ccaagcaaag cctcgctcaa tcgatcttca atttcttttt
     4621 gaagtgcgcg acggattgga cgtgcaccat attcaggatc aaatccagct ttcccgatca
     4681 catcgattgc agatggtgtg attttcactt gaatatcttg ttctttcaat cgtttgataa
     4741 tcgctttgct catgatttgg acgattgtat ggatttcatc tttactcaat gaatggaaga
     4801 cgacagtctc atcgattcga ttcaagaatt caggtcggaa agctttcttt aactcttcta
     4861 agatacgttt ttgcatggct ttgtgatctt tagtaacatc ttggacattg aatccgacat
     4921 ttttttcttc tcgaatttgt gtcgctccga tatttgaagt cataatcatg attgtatttc
     4981 taaagtccac ttttctacct ttagaatctg ttaaatgacc atcatccaat acttgcagaa
     5041 gtaaattgaa aacgtctgga tgtgctttct ccacttcatc taagagaatc actgaatatg
     5101 gtttttgacg aattttttct gtcaactgtc caccttcatc ataccctacg tatcctggag
     5161 gagacccgat taatcggctt gtactgtatt tttccataaa ttctgacata tcaacacgga
     5221 tcagggcatc ttcacttcca aacatgactt ctgaaagtgc tttggccaat tctgtttttc
     5281 cgacaccagt tggtccaagg aacatgaatg aaccaattgg acgatcagga tctttcaatc
     5341 cgcttcgtgc acggcggatc gcacgtgaaa ctgctttgac agcctcatct tgaccaacga
     5401 cacgttcatg cagaagtcct tccagttcta acaagcgttc gctttctttt ttctccaact
     5461 gctgtagagg gacaccagtc cactccgata caactgtagc aacatcttct tctgttacac
     5521 gatcagaata accttgctcc tgttttgctt caattgcaga aactttttgc agctttttac
     5581 gtagtgcttt ttcttgtctg cgtaattgag cagcattttc aaagtcttgt ttttggatcg
     5641 ctgcttcctt ttcttgtact agttcatcga tttctagttt gatcacagtt gattttgtta
     5701 agtgatcggt ttgatccaaa cggactttcg ctgctgattc atcgatcaaa tcaattgctt
     5761 tatcgggtag ttgacgagaa gtgatataac gaacagaaag attgactgct gctcgtacag
     5821 cttcatctgt gatctccact ccatggtgct cttcatatct tgtacgtaat ccttgaagaa
     5881 tcacttctgc ttcttcagga gttggctcat ctacttgaat gcgagcaaaa cgtcgctcta
     5941 atgcagaatc tttttcgata tatttttgat attcatctag ggtcgttgct ccgatcgttt
     6001 gtaattctcc tcgagctaac gccggtttca agatattcga agcatcaatt gctccttctg
     6061 ctccacctgc accaattaag gtatgcaatt catcaatgaa taagattact tgaccatcat
     6121 tatagatttc gtcgatgacc tttttcattc gatcttcaaa ttcaccgcga tatttcgtac
     6181 cagcaaccaa agatcccata tcaagcatca tcaggcgctt ttcttgcatg tcttctggca
     6241 cttcacctaa aatgattttt tgagctaacc cttcagcaat ggctgttttg cctacaccag
     6301 gttcacccac cagtacaggg ttattctttg tccgtcggct aagaatctgg ataagccgtt
     6361 tcacttctct cgaacgacca acaactgggt ccagcttgtt ttcttttgct aatttcgtca
     6421 aatcacgtgc taatgaatct agcgtaggtg ttccttgcgg tgtttgccgt ttatcttgta
     6481 catttacatt tctacgacga ttcattcctt ttccagcaga atcaccagct agccccattt
     6541 tttgtttcag aacttgacgg gcttttgaaa ggctgatacc taagttgatc atgattcgag
     6601 tagccatgat actttcatct tttaataagc tcatcaaaat atgttctgtt ccaacttttt
     6661 gagaaccagt tttcttcact tcgctgctag ctaaagcaaa aacttgtttc attcttggtg
     6721 aatatggaag aaagactcct ttagggtaag aacggatacc accataacca atcagatgtt
     6781 caatttcttc ataaatttcc gtttcattca aatccatttc acgtaatact tttccagcaa
     6841 ttccattttg ctcgaccact aaggctagta aaacatgttc tgaactaact gattgatgtt
     6901 tcaaccgttt tgcttcttct tgtgcaattt ctaatactgc ttgggctctt tcagtaaaaa
     6961 gttcttccat ctaatctcac ttcccatctt cataacttaa acgttccaga aatgctcgga
     7021 gaatccgagc acgtgtatga tcttcatatt cattgaaatt caatgtgttt tttgcaattg
     7081 cactcaacat caagtttcct tctttttttg taattatttg gtcttcataa agtttttgaa
     7141 taatagcaaa agcatttttc tcactgattg tatcatcaaa taactgattt atctgctcaa
     7201 gtaattgttt tttatctgaa atccttactt ttgcaattcg tatataacca ccaccaccac
     7261 gtttgctttc tactagataa ccacgttgga tggtaaaacg ggtattaatc acatagttga
     7321 tttgtgaagg aacacagtta aagaggtctg ccatttcaat ccttcgaatc tcgatcattt
     7381 cactattttc cagaattttc tttatatatg attcaattaa atccgaggta ttctgatgtg
     7441 ccatcttatt cattcccttt gactaatatt gaccttaatt ataacgaatt tgataataga
     7501 aagaaagaag aacgcttttt gttaaataaa tcctttagaa tcaacaaaaa aaaatacgga
     7561 atatactatt ccgtatc
//
LOCUS       DACEOM010000114         6991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEOM010000114 DACEOM010000000
VERSION     DACEOM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.120"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            155..493
                     /locus_tag="IW781_002754"
     CDS             155..493
                     /locus_tag="IW781_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1183723.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEHGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            493..885
                     /locus_tag="IW781_002755"
     CDS             493..885
                     /locus_tag="IW781_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAQ1183724.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQVSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            896..1408
                     /locus_tag="IW781_002756"
     CDS             896..1408
                     /locus_tag="IW781_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAQ1183725.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDD
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQAAVVQYTFK
                     DTVKATSTGA"
     gene            1454..1822
                     /locus_tag="IW781_002757"
     CDS             1454..1822
                     /locus_tag="IW781_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183726.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFIDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDNVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQAE"
     gene            1876..2163
                     /locus_tag="IW781_002758"
     CDS             1876..2163
                     /locus_tag="IW781_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183727.1"
                     /translation="MTNISDIERMTLYEYSVRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGISELSKQDKKFQSLLTKANS"
     gene            2180..5002
                     /locus_tag="IW781_002759"
     CDS             2180..5002
                     /locus_tag="IW781_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1183728.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGVKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTIQEAPGKAADWIKNKWTETKEFFSSIWDGIKEAASSAWEGIVNILAP
                     YVIAIKNVFQPMIDFFTNLWSQIGSIAGSAWEIIKTAVMGPILLLIDLITGNFNQLKE
                     DASMLWTTLTTNIQNIITTFVDIVVGYYTSLKDTVINIWNVLTSTIKDVWNSFTTWIK
                     ETTNNIVNSIKQGWNNLKQGTIDLFNNMIQGAKDLWNSFKAWFINLVIGTKDNIIQGW
                     ENLKQGTIDTFNNLVSGAQEVWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDS
                     FLEGLQNAWKSVQDFVGGIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFND
                     VQNTVGSMADFIAELFNANPDVDIAANLKNANKNIGAQVEHKVNMGGSTKPAVFKINL
                     GRQSFRLFMDDISQAMGEGADINLEF"
     gene            5035..5802
                     /locus_tag="IW781_002760"
     CDS             5035..5802
                     /locus_tag="IW781_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1183729.1"
                     /translation="MYSFKDTTINLNSSKRFLPTSAMMYDGMYLEDLIEGYQTLTVEG
                     REMLSVEVEQQDIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            5799..>6991
                     /locus_tag="IW781_002761"
     CDS             5799..>6991
                     /locus_tag="IW781_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1183730.1"
                     /translation="MSETIYFFDHLQKLIKRKNKRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPSETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPAVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPEATQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTELLKNTYSTLIDSARPLVQFKAEITGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRG"
BASE COUNT         2470 a         1040 c         1511 g         1970 t
ORIGIN      
        1 agtgtggaag aaggggcgaa ggtcattagg acgcagcctc ttttttctat ccctacattt
       61 gactatatcg agattgaagg aaagacttgg caacaaaaaa cagctagaaa tccagcatat
      121 agaaatagtt taattgtgca agaggtggtt cttgatgaag gcacaacttg aatataaagg
      181 aatcgatcag ctgatgcgac atctgaaaaa agcagcaacg cttaatgacg ttcaaaaagt
      241 cgtgaaaagt aatactgctg aaatgactga acgaatgcaa aaaggtgcgc cagtggatac
      301 aggttactta cgaagatcaa taaacatgaa tcttttagaa gctggtttaa ctggtattgt
      361 aggaccgaca gcagactatg ctccttatgt agaacatgga actcgcttta tgtcggccca
      421 gccctatgtt agaccagctt ttaattatca aaaagtcaaa tttatggctg aaatgaaagc
      481 cttggtgaaa tgatgattaa gacaagagat cagtcgattt ttgatgaact ttttaaaata
      541 tcccaaaaca aacttggata taaaacgtac gattacaaaa ctttagagga tgttggttat
      601 ccttttgtgg aatttgagaa cactcagaca atccatgaag taaataaaac tgacattaaa
      661 gggtctgtga ttgtggtttt atccgtttgg ggattacaga agaaacgaaa gcaggtgtca
      721 gatatggcat ctgctctttt taatgaagct agattgatag aagccacaga aggctattat
      781 tgggctttaa attatcaagc aagtggaatt caagtgatgg acgacacaac aacgaataca
      841 ccgctaaaac gggcggttgt cacacttgaa tttagaatta gataggagga agaacatgga
      901 agcattaaaa ggtattgatg tcattttgct ttatcgctta ttgaaaaaag aaactcagga
      961 agctgcttgg aaaatggcat ttcaaacaga acatgaaaat ggattatcaa gagattcaga
     1021 ctctacagtg acaaaagacg gaaacgttca aagtttaagc ccagttgaat atgatttttc
     1081 ggctacttca atagttgcca aaggcgattc tcatgtagat gaaatgaaac aagccttatt
     1141 aaatggcgat atcattgaaa tttgggaaat caacaaagca gaacagggca cagatgatga
     1201 tgcaaataag tacaaagcta cttattacca agcatatgtg tctgaattta ctccatcggc
     1261 tgctgcagag gataacgttg aattaagttt atcatttgca gtaaatggtg ttggtcaaga
     1321 tggttatgca accttgacag aagatcaagc tgctgtcgtt caatatacat tcaaagatac
     1381 cgtgaaagca acttcgacag gagcataaga gggcttagat gctctctttt ttattttagg
     1441 aggatgaaaa acattgaaat taaaaattaa aggtaaagaa tattcgttta aatttggcac
     1501 taaatttgta cgtgaattag acaaagtgat gcctttcatc gatggaaata tggaatttgg
     1561 aatgggactc tcagcaaaag tcttaccgga attacgttct tataatgtca acacgttgtc
     1621 acgagttctg gaaatagcaa atagaactga agatgaatct attacattag atgaattgga
     1681 tgattacatc gatgaagtta aagacattga aaaattgttt gataacgtct taaaagaatt
     1741 agcagagtca aatgcgggaa agttagcggt ccgaaacctg aatcagaaat tgaaagaagc
     1801 ggaaaaacaa caggcggaat agattctgcg cttgcatatg aacaaattct tataaattcc
     1861 tttcgatatt tgggaatgac caatatttca gatattgaaa ggatgacttt atatgaatat
     1921 agcgttcgaa tgactgcagc tcaactatct tggcttgata aagaaaaatt aattcacgaa
     1981 ttagcgtggg caaatcagca agttcaagcg gagaaaaaag taggcaaaaa gacagttcct
     2041 gtatatcgct cctttgaaga attctttaat tatcaaaaaa ttgaagattc gattatggga
     2101 atttccgaac tttcaaaaca agataaaaaa ttccaaagtt tactaactaa agctaactct
     2161 tgaggaaagg aggaaaaaca tggaacaatt ttctgttgaa gccttgttaa aagccacaga
     2221 tagtggattt gtaaagactt ttaaagatgc gcaagatgct gttaagactt ttgaaaagaa
     2281 ttcaaatagt atgacaaccg ctgttggtaa agtgatgcaa ggtactggtg ccgcaatgac
     2341 aaagtatatt accacacctc ttataggagt aggcgtagca gctgctaaag ttggtggtga
     2401 ctttgaagca caaatgagtc gtgtaaaagc tatatcggga gcaactggcg acacattcga
     2461 acagatgaaa cagcaagcga ttgatctagg agcaaaaact gcttttagcg caaaagaatc
     2521 agctgctgga atggaaaact tagcttctgc tggatttagc gcacaagaaa tcatgaaagc
     2581 aatgccgggt cttttagact tagcagctgt atctggaggg gatgtggctc tagcttctga
     2641 aaatactgct actgctttga gaggatttgg tttagaagca agtgaagcag gacatgtcgc
     2701 tgatgtattt gctcgtgctg ctgcggatac aaatgctgaa gttggagaca tgggagaggc
     2761 attgaagtat gttgctcctg tagccaattc aatgggtatt tctttggaag aaactgcagc
     2821 agctattggt attatgagtg acgcaggtat taagggttct caagcaggta caacgttgcg
     2881 aggagcattg tctaggttag caaggccaac aaaggctatg caagatacaa tggataattt
     2941 aggtgtttcg ttttatgatg ctgacggtaa aatgaaacct ttaaaaactc aagtagaatt
     3001 acttaaaaaa gcttttgaag gcctgacgcc tgaacaacaa caaaatgctt tagtaacact
     3061 atatgggcaa gaatcattat cagggatgat ggctttgatt gataaaggac ctgattcatt
     3121 gggcaaatta acaaaatctc tgaaagattc tgatggtgca gctgacgata tggctcggac
     3181 catgcaagat aatatgaatt cttccatcga gcaaatgttt ggagcttttg agtcagcagc
     3241 tattgtaatt caaaagattc tagcaccatc catcaaaaaa gtagcagatg ccatatccgg
     3301 cttagtagaa aaatttgtga gtgctccaga atcaactcaa aaattagtgg ttgccatagg
     3361 agcaattgtc gctgctatag gaccgttaat ttttatgatt ggttcagtaa ttatatggat
     3421 caatagggtg aaagtagctt ttaaagcttt aagtgaaagt tcaaaattgt ttagtggatt
     3481 aagtaaagca atgggtcttc ttacaaatcc ggtttttctg gttatagctg cggtagcact
     3541 ccttgtcgta ggtttcatct atctttggaa tacgagtgaa gattttagaa acttttggat
     3601 tggcttatgg gagggagtca agtctgctgt aagctcggca gtagaatgga ttcagaacgc
     3661 atggaaatct acaggagaat ggtttaacaa tttatggaag tccatcaaag aaggtgcaga
     3721 caatgtttgg actacaattc aagaagctcc tggaaaagcg gcagattgga tcaagaataa
     3781 atggactgaa acaaaagaat tcttttcgag tatatgggat ggcatcaaag aagctgccag
     3841 ttccgcttgg gaaggaattg taaacattct agcaccgtat gttattgcca taaaaaatgt
     3901 ctttcagcca atgattgatt tctttacgaa cctatggtct caaattggat caatcgcagg
     3961 atctgcatgg gaaattataa aaactgctgt aatgggtcca attctacttt tgattgattt
     4021 gataacaggc aattttaatc agctaaaaga agatgcttcg atgctgtgga ctacattaac
     4081 cacaaatatc caaaacatta tcacaacatt tgtagatata gttgttggtt attacacatc
     4141 cttaaaggat actgtgataa atatctggaa tgtgttaact tctaccatca aagatgtgtg
     4201 gaattctttt actacatgga tcaaagagac aactaacaat attgtaaata gtattaaaca
     4261 gggatggaat aacctaaaac aagggacaat cgatctgttt aataatatga ttcaaggagc
     4321 gaaagattta tggaattctt tcaaagcttg gtttattaat ctagttattg gaactaagga
     4381 taacatcatt cagggatggg aaaacctaaa acaaggcact atagatactt tcaacaattt
     4441 agtaagtggc gctcaagaag tgtgggataa tttagtaaat gctgttagtg atactgttga
     4501 tagagtaact ggctggtttg ataacttgaa aaatatcgat ttactagcag ccggaaaagc
     4561 catcatggat agttttctag aagggttaca aaatgcatgg aaatctgtgc aagattttgt
     4621 tggaggtatt ggtgattgga ttcgtgaaca caaaggacct atccaatacg atagaaagct
     4681 attgattcca gctggtcagg ctattatgaa cggtctgaat aaaggtctga caggaggatt
     4741 caatgacgta cagaatactg ttggaagtat ggcagacttt atcgcggaac ttttcaatgc
     4801 aaatcctgat gtagatatag ctgcaaatct gaaaaacgca aataaaaaca ttggtgcaca
     4861 agttgaacat aaagtaaata tgggtggctc tactaaacca gctgtattta aaatcaatct
     4921 tggaagacaa tcgtttagat tgtttatgga cgatatttca caagctatgg gcgaaggtgc
     4981 agacattaat ctagaatttt aggagggaat attttggatc agcgagaaaa taaaatgtac
     5041 tcattcaaag atacaactat taatctcaat agttctaaaa gattccttcc aacgtctgcc
     5101 atgatgtacg atggaatgta tttagaagat ttgattgagg ggtatcaaac actcacggtt
     5161 gaaggtagag aaatgctttc tgtagaagtt gaacagcaag atatacaaat tggttcaatc
     5221 attacaaatc agaaaatacc ttcaagaaca ctaaaaataa catacaagtt agaagacaga
     5281 gatccagaaa aattacagtt taaattcaaa gaactgttga attatttata ccggaatgaa
     5341 gacgtggaaa ttaggtttca tgatgaatta gattattatt actacggtcg ctatacatca
     5401 actgatactg ttccaggaga ctccaactcg attatttcga gttttaatgt attctgtgcg
     5461 gatccactaa agtatacgaa agaatgtgtt agtgatggct atattggaaa tccaatacag
     5521 tttcctataa caccaagaaa aattgaagtt actttatcca tgaataattc aatcaaaatt
     5581 acaaacggag aacaaaatat cacgataact gatgcggcaa taaaaacagg agacgtgttg
     5641 gtttttgatt tttccgatga gcaggtaact gtaaacggag aagattgtac ttctatgatt
     5701 gatttagaaa gtgattttga gaacttttat cttaagcaag gtcagaagat aactagcaat
     5761 aatgggaagc ttaaaatatt ctataggggg gcgacaattt gagtgagaca atttatttct
     5821 ttgatcactt gcaaaaactt attaaaagaa aaaataaaag aagtttaatt gaagtctccc
     5881 aagaaaaaga aattagttct gataagagtg atctaatgaa agatactctt tacgttacga
     5941 caaaatatga taaagaaata gaagatgcaa gatatatggc gattcgtgaa aacgagtcgt
     6001 ctttttcgtt gtatcgaatt actaaagtga gcgacccatc tgaaacatta gagtttacag
     6061 ggttaggatt tgcgacaaat gaattagatg cttacatcat caaagatatt aggccgagtg
     6121 ggcagccctt aaaaaatgtt cttgatcgat tgattgaatt tactgaagga aattggcgcg
     6181 ttggtcacgt agaagcaatg ttaccagcag taactgcaac tttttactat gtctctgtaa
     6241 aagaagcgtt gaaagaattg caaaccttag gtatggaatt tgtctttagg tgttctttga
     6301 attctgatgg aataaaggat aaatggatcg aagtatatga acaaattggt gaagaatcga
     6361 atacacgttt tgtatatggt agtaaagcat taacagttgt aagagagata gatcgaagct
     6421 ccatctcaac ttcaatgata ggtcgcgggc gaggcgaaga ggttggtgac ggatacggta
     6481 gaagaattga attcactgat gttgaatgga aaaagtcgaa tggtgatcct ttagataagc
     6541 ctaaaggcca aaattggctt gaagatccgg aagcaactca aaagtatggt ataccacaaa
     6601 aagatggatc aatgagaaag cgagaaaccg tggtagtgtt tgatgatata gatgatccaa
     6661 cagaattact taaaaatact tattcaacct taatcgattc tgctagaccg ttagtacaat
     6721 tcaaagccga aatcactgga ggagatgtga taggaaatac agtgactatt cacagatacg
     6781 ataaaggtta tcactataaa actcgtattt ataaaactac attcaatcgg cttaccggtc
     6841 aaacgaatat cgaactaggg gataatttaa cacaagatgt tagaaaacaa acggcttcta
     6901 ttgtcaataa tattaatagt ttagaatcta gcaaaatgac attttacgaa tcgacagaga
     6961 ttggaaaata tcaagatgac attatgcgag g
//
LOCUS       DACEOM010000115         6536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEOM010000115 DACEOM010000000
VERSION     DACEOM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.121"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..429)
                     /locus_tag="IW781_002762"
     CDS             complement(<1..429)
                     /locus_tag="IW781_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1183731.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQ"
     gene            984..1556
                     /locus_tag="IW781_002763"
     CDS             984..1556
                     /locus_tag="IW781_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1183732.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1788..2405
                     /locus_tag="IW781_002764"
     CDS             1788..2405
                     /locus_tag="IW781_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1183733.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            2633..2971
                     /locus_tag="IW781_002765"
     CDS             2633..2971
                     /locus_tag="IW781_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183734.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2956..3810
                     /locus_tag="IW781_002766"
     CDS             2956..3810
                     /locus_tag="IW781_002766"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1183735.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3957..4190
                     /locus_tag="IW781_002767"
     CDS             3957..4190
                     /locus_tag="IW781_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAQ1183736.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            4388..6292
                     /locus_tag="IW781_002768"
     CDS             4388..6292
                     /locus_tag="IW781_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1183737.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
BASE COUNT         2081 a         1091 c         1437 g         1927 t
ORIGIN      
        1 ctgttctgtt aagctagaaa cgaaggactt agagacggat ttaccacaaa gttcttccac
       61 aatttttgat actttacgag ttgaaacgcc tgatacatac atttccaaca ttgaagccat
      121 gagggctttt tcgtttcgtt gataacgttc aaacactgtg ggtgaaaaat ggccatcacg
      181 tgttctgggt acttttaatt ctagcgtgcc tacacgtgtc gtaaagctgc gctcataata
      241 gccatttcgt tgactttgtc ggttttctgt tcgttcatat tcttttgctt gaatatattc
      301 tgttcgttga ttttccatta gttgattaaa taccgttgtt aaaatatttt tagaaacgtc
      361 atcctttaca gaatattcaa taatgctttg aatctcttcg cttttcagtg taaaatgtac
      421 ttgggtcatg taaaagtcct cctgggtatg tttttgtcgt taaaaacatt gtaccgtaaa
      481 aggactgtta tatggccttt ttacttttac acaattatac ggactttatc ggtataccct
      541 aaatagacat cataataaca ttgaaaaaca tggttgtcat ttttctagat aaatggcata
      601 aattttcagc gctaagtgac aaaagcaact aatttttcat gagagatttt taaatatctc
      661 aatctatctc caaaattagt tgtttttgta gctatagttg actatgaata tgacaaataa
      721 ttagaaaaaa ctgtgccaat taagtataaa aatgacattt taggccctac tttaatgaaa
      781 tttttgtttg gagggaacga cttcattcct ttgattattt cagttgaatt taatagagaa
      841 gcctggcgtc tattaagaaa taattttctt gatgagcgtc aattttttta acggaaatct
      901 atcctatact aaattcagta aagaaaaagg aggaatcaaa gaaagctatt ttaaacaaac
      961 caaaattata ggaggaataa atcatgactg aaaataaagc tactcaagaa agaacacccc
     1021 aagaaagatt gcaagaagaa caagaacaca aggaacatgt tcatcatacg aaaattaatg
     1081 cagcggccat tgcggatcat cttttaggca acatgcatac attacatgtg aaactgcacc
     1141 aatatcactg gtatgtaaaa ggagccaatt tctttacgtt gcatgaaaaa tttgaagagt
     1201 tgtataacga aaatgaaaaa tggttcgaca aacttgcgga gcgtctaatt acttctgggc
     1261 ataaacctgc ttcgacaacg gttgaatttg aaaaatattc gatgctttcg gaagatgcag
     1321 ttaataaata cgctaaagca gaagacatgg ttgaaaatat tatcgaggac ttcagaagca
     1381 ctcgtgacct aactattcgc gcgattcgtt tagctcaaga cgagggtgac gatgctttag
     1441 aagatacatt gattgctttt aaaaatgact tagacaagaa catttggatg ctgcaagcgt
     1501 tcattggtaa agaagcatta gaagatgatg acgctcttga tgaggatgaa gattaaggta
     1561 aaagtagaag ctgatttagg gattggaatt ctttccagtc ctttttccat tttcttgatg
     1621 tgcatcaatt tttttgtagt aagagactta tataataaaa gcagcgaata taaacaaaat
     1681 aaataaaggg ggagaatgaa ttgactaaac atacccatca tgaaattggc gaccacgcag
     1741 cctgtatccg cttggttcca atattcaatc acttagagga tagcgcgatg aattacattg
     1801 ctgaaaaagc ccacacgaaa cagtatcaaa aaggagagtt tctgtttcgt tcacaagaaa
     1861 aagaggatac tttatatatt atcaatcgtg ggaaaatccg tatttatcga ttggcagact
     1921 ctggcaaaga gcagctagtg cggatcttga accctggtga tttcaccgga gaatggacct
     1981 tgttcaaccc ggatgcagta cacgaggaat atgccgaagc aacccgagat accgttgtct
     2041 gtatgattca gcaaaaagat gtccaagatt ttctggaaca atacccagct atttctttaa
     2101 aactattggc ggaaatgtct aaacggttag aaagttcaga acgtcaaacg acacaagtag
     2161 ccgtggagag tgtcattacc cgtttagttt tgtttttggc agaaaatgtg gaacctgaaa
     2221 tgggcaactc tcccaccatc accctgccga tggcaaaaaa ggacattgct tcctatttag
     2281 gaacgacacc tgagaccatc agtcgcaaat ttggagaatt ggaggacgaa ggattgattc
     2341 aacagctgtc tgggaaaagg atcaagattc aggatttaga tgatttattg ctttacagcg
     2401 aataatcgga cacacttttt tctacacgtt ggtgttctgt cgctcttcat taacaacggg
     2461 acagaactca gtgcttttgc ttccatctgt ccgtttttgg cttttttaag acagaacgac
     2521 cttcattcat tggaaaaata aaagcacaaa catgataacc ctcaagtttt tttgttttat
     2581 ttttctttaa gatagggttg tcgaacaaaa cgtaaaaata agtagggtga atgtgcattg
     2641 tttttgggtg atagttgcca tcacgttgtc agcagcgggg gcattctttg tcgtcccttt
     2701 caccaagaac acgattggcg gcgaagcaaa aacgtctaac gttgttgagg cagataggtt
     2761 agctatggaa aaagaagagg aagttgtaaa aggagccggc gtaatcggac acggaaaagt
     2821 ggcgtattac gaagcgagtg accagggaga taaaagtgta ttcactgttt ggtttccatg
     2881 gagcttagcc ggaatcttgt tcagcatcac catgactaga atgacgcgaa aaattgaaga
     2941 aatggagcaa atagaatggg attcgtatta ggcgttttga atccccatgt ggtggagatt
     3001 ggttctgttg tgattcattg gtatggagtc attattgcag cgggcatatt agcggctctc
     3061 cagttgagca ccaaggaagc aaagaaaaaa ggactggaag aggataccat tatcgatatg
     3121 gccttatggg caattccgat tggactcctc ggcgctcggc tttattatgt gttatttgaa
     3181 cttggttatt atctgcaaaa tccaggacaa atccttgcga tttggaatgg cggcattgcg
     3241 atttatgggg gattgattgc aggagggggt acgctatatt ggtatgcaaa gaaaaaaggt
     3301 acttctttgg cactcgttct tgatatttta gcgcccaatg tattgttagc ccagtccatt
     3361 ggtcgttggg gcaattttat gaatcaggaa gctcatggtg ggccggttac tcgtcaattc
     3421 ttggaaaatc tttatttacc tgaatttatc attgaacaaa tgaatatgaa tggtacctac
     3481 taccatccga cttttttata cgagtcattg tggagtttgt tgggctttgt cctcatcgtc
     3541 actatacgga accgaaaaca gcttttgcgt caaggagaag tcgctttgag ttacgttttg
     3601 tggtactcag tgggtcggtt cttcattgaa ggtatgcgaa cggacagttt atggattggc
     3661 gagtggatcc gcgtttcgca agccttgtct ctggccctgt ttattggcgc gattgtagta
     3721 tggtttatac gcagacaaga ttatccacca atttcttatt attctgatgg caataagggg
     3781 cagaaaaaga ctattaagat ggtgaattga gcaaaaagat aaaaagaagt gcaaaacttg
     3841 atatgcgtca attttcgttt gctaaaagtg agctatacta cagttgtaaa gaggaaaagg
     3901 cagtgttcat taatctgctg ccgataaaat aaaaacacat aaaggagata ggaattatgg
     3961 aaaaagctat attgcaatta gaaacgttgt cttgtccaag ttgtatgcaa aaaattgaag
     4021 gtgccgtgaa atcagttaac ggtattgata aagacagcat taaagtatta ttcaactcca
     4081 gcaaagtcaa aaccaatttt gattcagctg tcacttcgat tgaagagatt caaaaagcta
     4141 tcgaaaacgt aggctatccg gttcttaaag caaaagtcaa ggccgtttaa cagattaaat
     4201 gaactaggca attctggaag aaaagctatt tttccaggat tgcttttttt gttgcaatcc
     4261 gattcgaggt gcttagaaac gataaaagag aactttctaa acttgatgtc agtcaattaa
     4321 ttacttaggc aattatctta tactgtgttt atcaataaaa gagaaattaa atttggaggg
     4381 aaaaagcatg caacaatata tattaagcaa gaaaaatgtt atcaccgtca tcagtggatt
     4441 gttaatcgta ctcggtttct tcagtcactt tgttttagaa aacgtaggac tttcagagtg
     4501 gtctttgatc atcgcctctg tctttgggat tacgccaatt gccattcaag cgtttcaagc
     4561 aatgaaagtc aaagtcatca gtattgatgt cttagtcagt attgcagcga ttggtgcgct
     4621 ttttattcaa aattatgaag aatcggctat tgtcacgttc ttattcttat tcggacacta
     4681 cttggaacaa cgaacattga accaaacgcg atctgctatc aaagaattga ctgaaatggc
     4741 acccgaaagc gccttgaaac aaatggataa tggcgaattt gaagaagtag aagtggatga
     4801 tatagatgaa ggggatatct tgctcgttaa aacgggtgcg aaagttccag tagatggcat
     4861 agtccttacg ggtgaagggc atatcaatga agctagcatt acaggtgaag ctattccggt
     4921 caacaagcta gctgatgcag aagtttttgc aggaaccatt ttagaaaacg gaacgattca
     4981 aatcagagct gaccgagttg gtgaggacac cacatttgga aaaattattg aactcgtgga
     5041 agaagcacaa gattctaaat ccgaagcgga acggttcatt gatcgctttt ctaaatacta
     5101 cacaccagct gtcttggttc tggcaattgt tgtatgggcg ttcagtcaaa atattgagtt
     5161 agcaatcacc atcttggttc taggttgccc aggcgcatta gttatcggag tgcctgtctc
     5221 aaacgttgcc ggtattggga atggtgctcg taacggtgtt cttttaaaag ggagtgaagt
     5281 cattcaagac ttcagtaatg tggatacaat tgtatttgat aagacaggta ctttaactgt
     5341 cggaaaccca accgttgcag cgactgaact gtatgaaaaa gatcctgctg aaacgcttgg
     5401 ctatctggcc agtgtggaac gggaatcaga tcatccatta gcaaaagcgg tcttaaatca
     5461 aattggagaa acaaactttt atcctgttga aggaactgaa gttgtgaaag gcggcggaat
     5521 cgtttcaagt gtagctggac atagagtggc tgttgggaat gtggccttga tggaaaaaga
     5581 aaatgtcact ctcagcaaaa aagtgcaaaa agatgtcaaa cggtttgaac aacaagggaa
     5641 ctctctcgtt ttgacagcgg tcgacggtga attaaaagta ctgatgggca ttcgcgatca
     5701 agtccgtccg ggtgtgaaag ctaatttgca ggaattaaaa gacttgggcg tgaaaaatct
     5761 agtcgttctt tcaggagata accaaggaac cgttgatgtg gtcgccggcg aacttggttt
     5821 gaccgaagct cacggctaca tgttgccgga agacaaatcg gcttatatcg gaaaacttca
     5881 acaacgtggt caaattgtag cctttgttgg agatggcgtt aacgatagtc cgtccttagc
     5941 tttagcagac attggaatcg caatgggaag cggaacggac gtagcgattg aaacatccga
     6001 tgtcgtttta atgaactcga acttcagcaa cttgcctcac gcattaggat tagtaaaagc
     6061 caccgcaaat aatatgaaac aaaatatcgt gatttcagtt ggagtagtgt tggtcttgtt
     6121 gaccagcgtc ttcttcagcg aatggatgaa tatgtctatc ggaatgttgg ttcatgaagg
     6181 tagtatcttg gtggtaattt tcaatggcat gagattaatg aagtataagt tgaaaggtcg
     6241 gaaagaacaa aaagaagtat cagcacctac agagaaagta aaaatatctt aagaataaat
     6301 tagaagaggc tgagacaata gtccatcctt gaaatgacaa aagcggaaac tcagattttt
     6361 aaatctggga ttttccgctt ttttgagttc ttgaaaaaaa ataagagtat agagtatgtt
     6421 ctttataaaa ttaattttcg tgtaaattat taaaaattta ataataaaat ttaaattcct
     6481 attaagttgt aacacatgaa attgcttaaa atagtgtatc aaaaaccttg ttttat
//
LOCUS       DACEOM010000116         6361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEOM010000116 DACEOM010000000
VERSION     DACEOM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.122"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            148..408
                     /locus_tag="IW781_002769"
                     /pseudo
     CDS             148..408
                     /locus_tag="IW781_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            571..1236
                     /locus_tag="IW781_002770"
                     /pseudo
     CDS             571..1236
                     /locus_tag="IW781_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1272..1517
                     /locus_tag="IW781_002771"
     CDS             1272..1517
                     /locus_tag="IW781_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1183738.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1620..2489
                     /locus_tag="IW781_002772"
     CDS             1620..2489
                     /locus_tag="IW781_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1183739.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2470..3204
                     /locus_tag="IW781_002773"
     CDS             2470..3204
                     /locus_tag="IW781_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1183740.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3237..4145
                     /locus_tag="IW781_002774"
     CDS             3237..4145
                     /locus_tag="IW781_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1183741.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4142..4684
                     /gene="sat4"
                     /locus_tag="IW781_002775"
     CDS             4142..4684
                     /gene="sat4"
                     /locus_tag="IW781_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1183742.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4777..5571
                     /locus_tag="IW781_002776"
                     /note="aph(3')-IIIa"
     CDS             4777..5571
                     /locus_tag="IW781_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1183743.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5679..5867)
                     /locus_tag="IW781_002777"
                     /pseudo
     CDS             complement(5679..5867)
                     /locus_tag="IW781_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5847..>6361
                     /locus_tag="IW781_002778"
     CDS             5847..>6361
                     /locus_tag="IW781_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1183744.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2200 a          951 c         1393 g         1817 t
ORIGIN      
        1 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
       61 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
      121 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
      181 attgtattaa aaaaatcgtt ttatggttgt tcaaattatc ctgaatgtaa gtttacttta
      241 gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg taaaagaatt actggaggga
      301 aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa agccctacaa tgccgttgta
      361 aaaattgggg aaaagggata tattgatttt atctcttttt caaaataaac atggggctga
      421 cccaaaagta gctcctatat gaaaaataga ggtctttcca tttagatagt agtggaaaga
      481 cctctattta tcttttgaaa gtgaatacaa aaataaagca cactctgcta taatataggt
      541 gattaatcca tttgagagag gtgctttatc atgtatcaaa attataccat aaaccaactt
      601 tgtttgccta tagatcttga aattaaatta gaagaaaatg attttgctca tgccattgtg
      661 cagtttgttg actctattcc cgatgaagtc tttcttcctt attatcaatc tatgggtaga
      721 ccccaatatc atcctcgtat gctactttcc attattcttt gtgcctatat tcaaggggtt
      781 tattcgggcc gccaaataca aaatatgctg atagatagta ttcggatgcg ctacttatct
      841 caagagcaat tccctaactt tcgtactatt aatcgttttc gcgttcatcc gattatgaac
      901 gatattctag atcattcatt tgttcaattt cgtgagctac tggttcaaag tggcttaatc
      961 agtggcagtg ctctatacat tgatggtaca aaaattgaag cggatgcgaa taaatactct
     1021 ttcgtttgga aaaaaagtat tttaaagtat aaggatagcc ttgatcaaaa agcccttgaa
     1081 aactaccaac aaatggtgga aacaaaaatc ttacctgagt taatagatga tttaaaggat
     1141 gaactttcta tagaagagat tgaaaggatt cgtcaatctt tggaagaaaa ggaaaatcaa
     1201 ttaattgaag cgattgacca aaccgaaaca gtagaagggg gctgacccat tattaataag
     1261 gagtggacaa catgattgat gtaaatgagc tattggaaga agctattaga gaaactgaaa
     1321 atctaaatga tggcgaagtt tttcttgtta aagatttatt caagggatat gtggggaata
     1381 ggatacccag aaaggatcga cttctgcttg gaacgttatt tttaaaccat gttagcaaaa
     1441 tgaatggtaa tttaaaggct attgaaaaga cttcatctaa tcagcagagg tataaaaaga
     1501 caattgataa gttttaacta tcttttgtga aattaaaaaa attataaata tgttgagtag
     1561 aagcaaaaga tttggcttgg aatttttaca gcagtgaatc gataaaagga gcatgcttga
     1621 tggtagataa tattattaaa tcagtagcag agaaattatc ctctctgtct tatatagaag
     1681 gtattgtttt aggtggttca cgtgcaaggg gcacccatac agaggattcg gatatagata
     1741 tcggcatcta ttacaattca gaatcatttg acataaatac tattaatcaa ttcgctacaa
     1801 agctggatga tgagcataga aataaccttg ttgtacctcc cggagcatgg ggtgattgga
     1861 ttaatggcgg cggatggtta gtcataaacg ggtatcatgt ggatttaatt ttacgtgata
     1921 ttaaacgtgt ggaacaaata atgaaagata cagagcacgg aattgttact gccaattatc
     1981 agactgggca tccccatggt tatattagtg caatgtatcg aggagaatta gcgattagca
     2041 aaatactata tgctaagaat gaaagcttat gcgaattaaa aaaacaggca gaaacttatc
     2101 ccaatgcttt gcagaaaagt ttagttaact tttttatgtt tgaagcaggg ttctctttaa
     2161 tgtttgtaaa agcaaattcg ggaacagacg ataaatatta tattgcgggt catgtttttc
     2221 gtatagtttc atgtttaaat caagtgttat ttgcatgtaa taatgcttat tgtatcaacg
     2281 aaaagaaagc tataaaactg cttgaaactt ttgaacataa acctgaaaaa tataccgaga
     2341 aggtaaatca tatttttgaa gtactcggta tctcactttt tgaatgctac gacatgaccg
     2401 agaagcttta taatgaagtg aatgaaattg tatcggagat aaataacttt ttaaacgagg
     2461 agagttcaga tgaaagaaaa caaatatgat gataatatat tttttcaaaa atacagtcaa
     2521 atgagtcgct cgcagaaagg actggctggt gcgggagaat gggagacttt gaaaaagatg
     2581 ctacctgatt ttaagggtaa gcgtgtgctt gatttaggat gcggctatgg atggcactgt
     2641 atatatgcga tggaaaacgg tgcttcctct gtagtaggtg ttgatatttc tcataaaatg
     2701 ctcgaagtag caaaaggaaa aacccatttt ccacagattg aatatgaatg ctgtgccata
     2761 gaagatgtgg atttcccaga ggagagcttt gatgtaatac taagttcgct tgcgtttcat
     2821 tatgtagcag actatgagaa tttaataaaa aagatatata ggatgctgaa ggctggtggc
     2881 aatttagttt ttacagttga acatcctgtt tttactgctc atggaacaca agactggtat
     2941 tataacgaaa aaggagaaat actgcatttc ccggtggaca attattatta tgagggcaaa
     3001 cggacagcta tgtttttgga agaaaaggtt acaaaatatc atagaacact gaccacatat
     3061 ctaaatacac tgctttcaaa tagttttata ataaatcaga ttgtggagcc acagccgcca
     3121 gagaacatga tggatattcc ggggatggcg gatgaaatgc gacgcccaat gatgctgatt
     3181 gtatcggcaa aaaagaagat gtaataatat agaaaaaata aacgaggagt atgtaaatga
     3241 gatcagaaaa agaaatgatg gatttagtac tttctttagc agaacaggat gaacgtattc
     3301 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     3361 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     3421 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     3481 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     3541 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     3601 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     3661 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     3721 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     3781 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     3841 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     3901 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     3961 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     4021 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     4081 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     4141 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     4201 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     4261 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     4321 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     4381 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     4441 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     4501 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     4561 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     4621 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     4681 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     4741 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     4801 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     4861 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     4921 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     4981 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     5041 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     5101 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     5161 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     5221 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     5281 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     5341 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     5401 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     5461 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     5521 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     5581 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     5641 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     5701 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     5761 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     5821 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     5881 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     5941 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     6001 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     6061 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     6121 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     6181 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     6241 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     6301 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     6361 a
//
LOCUS       DACEOM010000117         6281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEOM010000117 DACEOM010000000
VERSION     DACEOM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.123"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(108..680)
                     /locus_tag="IW781_002779"
     CDS             complement(108..680)
                     /locus_tag="IW781_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1183745.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(687..1349)
                     /gene="rpe"
                     /locus_tag="IW781_002780"
     CDS             complement(687..1349)
                     /gene="rpe"
                     /locus_tag="IW781_002780"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1183746.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1365..1880)
                     /locus_tag="IW781_002781"
     CDS             complement(1365..1880)
                     /locus_tag="IW781_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1183747.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1882..2166)
                     /locus_tag="IW781_002782"
     CDS             complement(1882..2166)
                     /locus_tag="IW781_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1183748.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2200..3528)
                     /locus_tag="IW781_002783"
     CDS             complement(2200..3528)
                     /locus_tag="IW781_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1183749.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3542..3997)
                     /locus_tag="IW781_002784"
     CDS             complement(3542..3997)
                     /locus_tag="IW781_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1183750.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(4000..5946)
                     /locus_tag="IW781_002785"
     CDS             complement(4000..5946)
                     /locus_tag="IW781_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1183751.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2066 a         1209 c          876 g         2130 t
ORIGIN      
        1 ctcaaaacta ctgaattatt aagttatgtc taatcaaata aaaaaaccat tctcatattt
       61 gctgaataat tagcatttag agaatggttt tttatcttta aaatctacta ctcaaaatct
      121 gcatgtcgag caaacatcgt ttctgagctt tcacctgttt tatccattaa agataacact
      181 aaactatcaa aggtgagaaa tgctaattgt tcaaaacttg aacccatagg ttgtgaaaaa
      241 gtatcttctt ctcgttgatt ttcatctttg gtaatacctg gtaatacaag aacagaatct
      301 gctaattgac caatcgacga gtcttttttc atggtaacta aagcaatgct tacaccactt
      361 tgatttgctt tggccgctaa attttttaaa ctacctgttt cacctgagcc agaacagata
      421 attaataaat ctccaggttg cgaatgtggt gaactaattt caccgacaag gctgactgaa
      481 aaacctaaat gcattaagcg attcgtaaat gcttgcatgg caattccaga gcgtcctgca
      541 ccagctagaa agatgtgttt agcttgaaca attttctcac taaattgttc taactctaca
      601 gaattaatac gttttgagtt attttgtaat tctaagataa tcgcttctaa atagttttta
      661 ttttggtctt tattggtcat gatcttttac ttcacttcct ctaatgattg atttagttta
      721 tcaatttgtt cttgcggggt atttttccca ttgaagatga aaccgcctga tacaaaaata
      781 tccgcccctg ctttagaaca aactttaatc gtttgattat caatcttgcc atctacttca
      841 atatcatacg tataatcatt ttgtttgcgt attacatcta actgttcgat tttaactact
      901 gtctcttgaa taaaatgctt attctctgtt cctggattga ttgtcattac taatacttga
      961 tcgactaaag ataatactgg ttcaataaaa ctgactggtg ttgcaggatt aaaagtaatc
     1021 cctacttttg ctcctgcttc cttgactaac tgaatgacct tcattgcgtg tttggtagct
     1081 tcaatgtgga aggtaacaat atctgctcct gcttctacaa atcttgtgac aattttttct
     1141 ggtgtatcaa tcatcaaatg aacatcaaac acaacgtctg ttagtggacg aatatcttta
     1201 ataacacttg gtccaaaagc catatctggt acaaaacagc catccattac atcaatatgc
     1261 aaataatcaa cattcttttc ttttaagatt tcaaataact ctttaacttc tgcgataggt
     1321 gcatttaaca atgatggagc aattgtcatt ctctattccc ccgtttattc tcccataatc
     1381 atgagattga ttgttctagt tcgctctctt agttgatccc aatcgacaaa atagacacgg
     1441 cccatttctc ctaataaaag tttaccttct tgaacaatta aagtcattga gttggttcca
     1501 taaatagatg aacgtaaatg tgcatcggta tttagcatga cccatttttc tgcagggtaa
     1561 ttgggatcag ataatcttgt tccaaaatca atgtgttttg gccccggact gttgtattga
     1621 ttttcactcg tcatttttgg aataatcgtt tccattacat tgttgatgtc tacatgtaaa
     1681 taatcatcac caaaaaagtt tgtgtcatgc atcgtttctt caaaaaagag tgagcaagtt
     1741 gtatgcgggc tcgaaattac taacattcca ttctcaatac ctgctgtatc aactgtatct
     1801 aaaagttctt ttcgaatatc atgatacgaa ggacgccctg aaactgtttc aatagtcaaa
     1861 gtgtcatgat gaaaacgcat attaaacacc taatctttct tttacatctg cataaatggc
     1921 atctgtacca atacctgtta ataagcccaa gccattcgtt actttagctt taattacatc
     1981 agggacaatt gttgtactta ctacagcatc atattcatca attttattta gctcatcagc
     2041 aattttagat tgataaattt tcacttgatc tgttaatcct tcttcttcca accattttga
     2101 taattttgct actgcaattg tagaagtagc atgacctgct ccacaaataa ctaataattt
     2161 tttcatattg atctcttctt tcttatttta attttatgat taagctttct tcactttgtt
     2221 catgtagata gaagcaccta ctaaaacaac tcctaatcca cctattccaa ttacaccaaa
     2281 gtaagatgct aataaaacaa acaaccctac tggccataat ccacataaca acatagttac
     2341 taatcctgct gtccccacat cgaaacttgc taatgtaaac acatcagtga taattggtgc
     2401 caaccatgtt gccatatata atgttgtaac aacatacaac gaacatccta ctaatgatct
     2461 cacaatattt cctttaaaat aaggaatcat taaacagatg gcgaaagcaa agaatgctaa
     2521 atctccaaaa ggtaatactt tatttcctgg aagaattagt gctagcaata atgagattgg
     2581 tacaagcaat agagaagttg acataatagt tggatgaccc acagttaacg ctgcatccat
     2641 tccaatgttt acttggcgac catttaactt tttattcgta aattctttcg ccgcttctgc
     2701 aattggcatt aaaccttcca taaacattgc taccatttta ggcataatct tcatcactgc
     2761 cgcagtcata attcctaagt ttccaattcc tgctacgtca tagcctgcca taatacccac
     2821 tacaataccg ataattaggc caataacaac tgtgtcacct aaaacaccga aacgttttgt
     2881 taatgtttct gcatctgcat ctattttatt aaatccagga atacgatcaa aaacccaatt
     2941 tactggtttg gcaaatagta ctcctgataa tcccatcata tgtgtgatag agattcctgg
     3001 atagccgaaa aattcgctaa cagttttttg tgtacgatct gctaagaacc attgtaaaat
     3061 tactgcgaca atcgcgccaa ttaagccaaa ccaaaagttt cccgtaacag attgagtaat
     3121 catacctagg aaaattgcga accaaaagtt ccaaatatca acattcaatg tttttgtcca
     3181 acccaaaacc acaaatatga agttaagaat taatgaaatt ggaatcatta aaattcccaa
     3241 tgatgtagag taagccaaag gtccaccaac acctacgcca atatctagga tattcaggcc
     3301 accaccgtat tgttccccca tttgttgaat tgctgttcct aaaccatctg ataataaact
     3361 taaaactaat cctaaaccaa tagaaccgat accaacagta acgccggaaa caaatgcttt
     3421 accaggtttt aatccaaata atatcccgat tacaaaaata atcagtggta aaaagacgtt
     3481 tgaccctaat ccaataaacc attcaaaggc atttaataca atatccatct ctctattcct
     3541 cctaataaac tcctgcagaa tgtactaact caataaatgc ttctttcgtg gtacatgcaa
     3601 gtaattcatt tactttttct tcattttgaa ataggctaac taagttttgt agtgtatcaa
     3661 tttgttcatg aggttgtgac attgccaaca taaagactaa acgtacaggt atttcatgag
     3721 atttatctgt catatctgaa aataccagtg gttcctttaa tgaggcaaaa cagatttgag
     3781 agctatttac atgtgcggaa tctgtatgtg ggatagcaaa cccaatttga tttacttcta
     3841 atccagttgg aaattcttgt tcacggttgg tgataccttc taagaaacta tctttcacgc
     3901 aaccagcgtt ttttaattgt tcagacatta cttgaaacaa ttctttttga tctttcactt
     3961 ctaattgaaa taaacataat tctttatcta gatacatttt tattctcctt tttgttgaaa
     4021 aattcttagt aatttttcgc tatcatgaac ttgtaacaag cgatcgacca tttctttttg
     4081 cccaataaat tggtaaagtt cttcatatac tcctctaatc tcagatacat cttcttcagc
     4141 taaactaacc ataatgacta atttgacttt tcgatcgccc caattaatcg cattttttaa
     4201 tgctacaata aaaatgttgg attcaatgac cattgatgga ttcgcatgcg gtaaagcaac
     4261 accactttct aaattagtat tccccatttt ctctctagag aagatagaag cacgaaactc
     4321 ttcagtaact aatgaattgg cttctagttc ggaaatcatc aaatccaaac attcttcttt
     4381 gctttccacc tgtactcctg tttttactaa agatgattga acaaatttag atatggtggg
     4441 aactttaacc gctttaggaa tcagttcatt ttgaataaat cgcgaaggtt cttctattaa
     4501 atatttagta taagcttcca taatattcat ataatctgtt ttacttacca atggagatac
     4561 ccttactaag ggtataccac ctgcatcaaa ggaaagagag gaaataatca aatcaacttc
     4621 cccaatttca gctaaatgaa acttatctaa agttgatatt tctacattat cttttgctgg
     4681 taaaaaccgt cgaactttat tgtaaattaa ttgcgcagaa gaaataccgt attggcaaat
     4741 aatgatgata ttatttgctt tggactgatc ttcaatagca atctgaaaat gaatcaaaat
     4801 taaggaaact tcatcgtccg ttaaagtcac atcaaattct tcttcaagga tggacatggc
     4861 gtaccatact atgctaaaga gttctgaata ttgagatttg atattttcta ataacggatt
     4921 ttgaatttta ataccttttt ttaaacgaag gaccattgct ggcacatgat acaataagga
     4981 ttgatataat cgatcatttt gagtcaaatc aattttttca atctcactca ttctttcaat
     5041 caatttatga acgctcatag cgtactcttc attgctggtt tttaaattat ttgtcacacg
     5101 atgtgcaaat agttgacgac atagatattc tttatctgcg tcataaaatg gaagattcaa
     5161 ttttgccttc aattcttcaa ttaaaccatt tgccacaatg tacgtttcca tatagcgaat
     5221 gctattgaag aggaattctt cttcaacagt taaatgatga cctttcagta aacggttaca
     5281 ttggataact aaagtagaaa tcaatgaccg aacatagtaa tcagaaacct tttcagtcag
     5341 ttcactgtaa tcattgaata gcaaatcatg aatgacacta ataacttttg aatcaaacaa
     5401 catatttaaa acttgaaaaa atgtttttga ttccatgtca ttcgcataat agaatacaag
     5461 ttgcttgatt gctcgttgaa ttgcttgttc attaccagta aaactaattc cttctgcaga
     5521 tgactgcaac ttaacttctc cactgccaat aattttattg ataattctca ggtcattgta
     5581 taacgatgtt ttactaacca taaaactttg agacaaatga gttaaagaaa caggcttatc
     5641 accaaacaaa acttctttaa tgatctcgac acgtcttttt tctggagaat aaatatcttt
     5701 ttcaagataa ttttctaccg atgaaatcaa atgttcaacg tttttcctat cacctatgag
     5761 tttgataccc aaagaaggta atcgctcaat tgttacttgg taattaacta cataatcttc
     5821 caacaattca atatcttgat aaatggtctt ggtcgacact gccaatttat cactaaaaaa
     5881 agaagcgggt ttaaagtctt cttgtcgact taataatttt aataaattta catgtctttc
     5941 aatcaaactt gttcacctct taacgctagc atttgttacc tcttttcatt atttattcta
     6001 gcactggaac ccccttccct caattacatc tatgtcaata atacttgtgg aagtttgttg
     6061 taaaggaaat ccaaataatc ttaaaaaatg agaaacaaaa aaggagaaat ccttaaaaga
     6121 gctaagatat tccaatagaa aatactcctt attatggaag taatatgaaa tattacaatt
     6181 gaaaaataaa aacaatataa tggcgtcact aaaattcgta tttttttatt ataatataac
     6241 ttaattacaa atgctcatct atgaacaatt gagtcctaat t
//
LOCUS       DACEOM010000118         6219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEOM010000118 DACEOM010000000
VERSION     DACEOM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.124"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..284
                     /locus_tag="IW781_002786"
     CDS             <1..284
                     /locus_tag="IW781_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1183752.1"
                     /translation="EFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPK
                     SMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(370..1464)
                     /locus_tag="IW781_002787"
     CDS             complement(370..1464)
                     /locus_tag="IW781_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1183753.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1653..2414
                     /locus_tag="IW781_002788"
     CDS             1653..2414
                     /locus_tag="IW781_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1183754.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2529..2969)
                     /gene="fabZ"
                     /locus_tag="IW781_002789"
     CDS             complement(2529..2969)
                     /gene="fabZ"
                     /locus_tag="IW781_002789"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1183755.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3189..3944
                     /gene="fabI"
                     /locus_tag="IW781_002790"
     CDS             3189..3944
                     /gene="fabI"
                     /locus_tag="IW781_002790"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1183756.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(4054..5067)
                     /locus_tag="IW781_002791"
     CDS             complement(4054..5067)
                     /locus_tag="IW781_002791"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1183757.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5232..6152
                     /locus_tag="IW781_002792"
     CDS             5232..6152
                     /locus_tag="IW781_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1183758.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1913 a         1235 c         1109 g         1962 t
ORIGIN      
        1 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
       61 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
      121 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
      181 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
      241 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
      301 aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata
      361 actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc
      421 aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt
      481 catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat
      541 cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta
      601 gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg
      661 aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt
      721 caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc
      781 gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg
      841 tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca
      901 agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct
      961 gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata
     1021 aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac
     1081 gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat
     1141 ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt
     1201 atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat
     1261 atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga
     1321 aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc
     1381 atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata
     1441 aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat
     1501 ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa
     1561 taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag
     1621 ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaagta
     1681 ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac acgacttctt
     1741 ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg actgccagta
     1801 gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc gaaaatgaag
     1861 atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt cccgaacacg
     1921 caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac aacggactga
     1981 ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa ggcgatggca
     2041 ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac cacctttata
     2101 atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac ggcagccagt
     2161 tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac gactatcctc
     2221 aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc cattatactg
     2281 tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat atcgaaacag
     2341 atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata ctgcaagaag
     2401 caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt atgctaggat
     2461 tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg ctatatcagg
     2521 ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca gactttttta
     2581 tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt ggtgatttca
     2641 aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc cttattgatc
     2701 ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag tatcgaacct
     2761 gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc aggaaagtga
     2821 ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt ttttcctggt
     2881 tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat caattccatt
     2941 acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg caattgccat
     3001 tttcattact ttgactatca aagtatcata ttcctaaata tttagcaatc tttttttaat
     3061 ttaagaaaaa atagtttgac aatcaaaaca ttttgactaa taattaagaa atcaagaagt
     3121 tattttagct attattctct atttttttac gactatcaaa tttttatcag aaacaaagga
     3181 gagtcattat gtcatttcta gaaggaaaaa aaatcgtcat tatgggcgtc gctaacaaaa
     3241 aaagcattgc ttggggttgt gctgaagcaa tgttgaaaca aggggcagaa attatctata
     3301 cttaccaaaa tgaacgaatg aaaaaatcat tagtcaaatt ggtcggcgaa gaagcattca
     3361 ctgtagaatg tgatgtttct agcgacgaaa gcatcgaaaa agcaatgaat gccatcggtg
     3421 aatatgctgg ggagatccac ggccttgtcc atgcagttgc ttacgccaac aaagaagaat
     3481 tgtccggaaa tgtgtctgat atttccagag acggctatca attagctcaa gatatcagca
     3541 gctactcttt aattgctgta tcaaaatacg cacaaccgtt tcttgcacca aacagcggga
     3601 tcgttagcat gacttatcta ggttcagaac gcgcaatccc gaattataac atgatgggta
     3661 ttgcgaaagc ttctcttgaa gctgctatcc gttatttggc agctgagttc tcgcctaaag
     3721 gaattcgggt caatggaatc tcagctgggg caatcaaaac attagctgtc acaggtgtta
     3781 aagactatca aaaactgatc cagttatctg aatctcgtac gcctgatcag caaggtgtga
     3841 cgatcgaaga agtcggtaat gcttgtgctt tcttaatcag cccactttcc agcggaatca
     3901 tcggtgatat tatctacgtt gacaaaggtg tccatctttc ataaactttt gttgcattct
     3961 ttaaagtaaa acatataaaa atcaggaaaa aactttttaa agtctcttcc tgattttttc
     4021 tttttacgtt tttttcaact cattactttg aaattattcc acgacttcaa tccagccttc
     4081 tggtgcttct atgtctccaa attgaatacc tgtcaattca tcatacaacc gttttgttac
     4141 tggtcctact tccgtttcac tataaaatac atgaagattg ttattgtgat aaatcccacc
     4201 gattggagaa ataacagccg ctgtaccaca tgcaccagct tctgcaaatt gatctagatc
     4261 atcaatgaaa atatctcctt caattgcttg cagtcctagt cgttcttcag ctaactgcaa
     4321 caaggaatat ttcgtgatac ttggtaagat agagggcgat tgcggagtga tgaattttcc
     4381 gtcttttgta attccaaaga aatttgctgc acctacttct tcgattttcg tatgtgtggc
     4441 tggatcaaga taaacacaat ctgagtaatc cagttctttt gcttctgttc ctggaagtaa
     4501 actcgctgcg taatttccgc cgacttttgc tgcaccagtc cccttaggag cagcacgatc
     4561 gtaatcagat acaataaaat tcgttggttt tagtccacct ttgaaatacg ctcctactgg
     4621 cgtacagaag acgacaaata aatattctgg agctggatgg acaccaatgt tttcaccgac
     4681 cccaatcagt aaagggcgca agtataatgt agcgcctgtt ccatagggcg gcacaaaatc
     4741 ttcatttgct tttaccactt gcttgacagc atcaacgaac atcttttcag ggaccattgg
     4801 catcagcaat cgtctagcac tttgattcat ccgtcgacta ttttgttcag gtcgaaagag
     4861 attgaccttg ccgtcttttc gacgataagc ttttagacct tcaaaacatt gctgaccata
     4921 atgaagcgct ggagagcctt catgaatcgt caggtaatta tcttctgtca attctccttc
     4981 ttcccattgt ccgtctttcc atttggcaat aaaacgccaa ggagttttga tataagaaaa
     5041 acctagattg ttccaatcga tttgcatata cttaccacct tcgttatttt ctccacattt
     5101 tatcattatc catattattt gtcattttat tttcagcttt tttccttcat tatagttatt
     5161 tttgctattt ccttttttga aaacttcttg gtacaataca gaaagaatca aattttagaa
     5221 aagaggattt tatgttttca ttattagcaa ctgccggtac gacagactct tctggcgaga
     5281 cattgacaga tcaagctgtc agcaaagtaa atgcgtttca gcggttttgg gacgggattg
     5341 attgggacgc cattttggct gcctttatcc aaaaaagttt atatctgatt tttttgatcg
     5401 tccttttttg gattcttcac cgtgtgggaa aatatctgat tgataagtct tttaatcagt
     5461 attctaaaaa agccattcta aacgagagcc gcttaaagac tttacatagt cttttaaata
     5521 ctatttttca ttataccctt ggtttctttt ttatctatgc aattttatct gctatcggtg
     5581 tgccgatcgg atcacttttg gctggtgccg gaattgcagg tgtggcaatc ggtcttggtg
     5641 cccaagggtt catgagtgat gttattaccg gattttttat tatcatggaa caacagatgg
     5701 atgttgggga ctacatcaag ctagcaaact taacgattga aggaaccgtc gtatctgttg
     5761 gtatccgtac ccttcagctg aaagcaacgg atggtactgt ccatttcatc ccgaatcgaa
     5821 acatcaccac gatcagcaat ctctctcgtg caaatatgca agtactttta gatattcgga
     5881 ttgttcctga agaaggatat gacaaaatct atgagatcat tgatcgagta aatcgtacat
     5941 tagctgaaaa ataccaagaa gagctccaaa ctgaaccttc tatctttgga ttagtggatc
     6001 ttggaaacag caattttgcg attcgtacgg tctgctacgc attgaacgga aaacaatatg
     6061 ccttaaaaga agagttcttg agctcctatg tcaaagaatt gacagcttct ggtttcacga
     6121 ttccgaacag tccaattgct gtcggaaaat aatttggaca aacgatgcaa cgaatctcac
     6181 taaaaagagc gtgggacaaa agtaaagaac acttttgtc
//
LOCUS       DACEOM010000119         6174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEOM010000119 DACEOM010000000
VERSION     DACEOM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.125"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            155..538
                     /locus_tag="IW781_002793"
     CDS             155..538
                     /locus_tag="IW781_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183759.1"
                     /translation="MSKRKVFQINGLSGLSKKLKKNATMDDVKTVVKVNTAELTEAMQ
                     RETQKVLTGHWEGGKFVKPTGATKRSITMKLSNKGLSGHTGPKTEYSPYLIRGTRFMA
                     KRDFFLPPLRIQKAKFKSDLERLVE"
     gene            539..934
                     /locus_tag="IW781_002794"
     CDS             539..934
                     /locus_tag="IW781_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAQ1183760.1"
                     /translation="MKTREQSIFDEMFKRSNALGYQTYDYKPASAASYPFVEFEDTQT
                     LHSANKSHVLGNVVIVISVWGLHTKRKQVSEMASALFEQAMQVNKSDGYSWMLDTNAS
                     DIQTVTDTSTNTPLKRAIIELNFRLIGGI"
     gene            935..1432
                     /locus_tag="IW781_002795"
     CDS             935..1432
                     /locus_tag="IW781_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAQ1183761.1"
                     /translation="MALKKGIDVILVYRDLEKQAEEAAKTVTYQTEHTFGMSRSTDAT
                     ETKDGTAQTVGAIEYDFSSTALYERGSKTLKMLYDAFMNNKLVEVWIIDKLEPQEGDT
                     GKFAAKYMQVYISSYEETASAEDNVEVSLEYAVQMIHQDGYATLTTEQQNAVQYAFVD
                     TTKQT"
     gene            1465..1812
                     /locus_tag="IW781_002796"
     CDS             1465..1812
                     /locus_tag="IW781_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183762.1"
                     /translation="MELTINNKEYSFIFGFGFIREMNRRYSVVEQGMTMKLGLDSTLV
                     NFFNEDIETLIEMLKVANATESPRVAEKDLIALVGEIGSDKLFDLVLEELKKSEFTKK
                     KTLTVENRIKESK"
     gene            1863..2123
                     /locus_tag="IW781_002797"
     CDS             1863..2123
                     /locus_tag="IW781_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183763.1"
                     /translation="MTDFLDIDRMTMTEYETRLIAYRLKRLDEQELIHYQAWANNQVK
                     ATKKRGKYEVPLFDTFEKFFNKEKLENKILGKDEKVPRFVNS"
     gene            2140..4185
                     /locus_tag="IW781_002798"
     CDS             2140..4185
                     /locus_tag="IW781_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1183764.1"
                     /translation="MESYSVEAILSAVDKNFTSTMKNADSSMSGLNDNTKKTNTSILD
                     IAKGIGVFKLVDSAIGLVKSSMDGAIDRFDTLNKYPVVMQALGYSAEEVDKSMNKLTD
                     GIDGLPTSLDEIVSSAQQLAISTGDLQKGTDTALALNNAFLASGASTADASRGMQQYQ
                     QMLAKGTVDMQSWRTLQETMPIAMDKVAKSFKDQGVNSVNELYDALQDGTITFDDFNN
                     RLIKLNDGVGGFAELAKKNSAGIRTSFSNIKTAVVKGLANVITAIDEGMQNAGLGSIA
                     ENFDKIKNAVNVAFKAITDSIPPAISFLTNLWDTIKPFLPLIMAVVGYIGIYQAVMGT
                     ARKAVELYNGAQKMMNVLMNLNPIGLVIAAVIALVAGFIYLWNTSEEFRNFWIGLWEA
                     IKTATGAAVDWIVSAWEGIKQFFVDTWESIKQGTIDAWNALKTWLSETWDSMKQGAID
                     TWNSVKQFFIDLWESIKTNTINMWNAIKDGVTTAWENTKNAVINTAKSIVDGAAQAWE
                     DMKTGVSNAVDRVKETFDTIRQIDLLQIGKDIIDGLVNGIRSKIDDVVNAVKDVAGSI
                     TGKIKDVLNIHSPSRVMAELGMFTSQGLAEGMLDGARYVDRASSQIADRASNMDIGNR
                     ISAVNSQIQTQVQHEVSYGTNSKPAVFKFNLGRQSFRLFMDDISQAMGEGADINLEF"
     gene            4218..4985
                     /locus_tag="IW781_002799"
     CDS             4218..4985
                     /locus_tag="IW781_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1183765.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLKVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            4982..>6174
                     /locus_tag="IW781_002800"
     CDS             4982..>6174
                     /locus_tag="IW781_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1183766.1"
                     /translation="MSETVYFFDHLQKLIKRKNKRSLIEVSQEKEITSDKSDLMKDTL
                     QVTTTYDKEIEEASFMAVRENKSSFSLYRITKISDPAETLEFTGIGFAPNELDAYIIK
                     DIRPSGEPIKSVLERLIGFTEGNWRVGHVDAMLPAVTATFYYISVKEALKQLQTLGME
                     ILFRCSLDADGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDIEWKKANGDPLDKPKGQNWLEDPDATQKHGIPQKDGSMRKRE
                     TVVVFDDIEDPKELLKNTYATLVESARPLVQFKAEVVGGDVIGNIVTIHRHDKDYHYK
                     TRIYKTTFDRLTGKTTIELGDNLTKDARKQTASIINNINSLESSKMTFYESTEIGKYQ
                     DDIMRG"
BASE COUNT         2188 a         1030 c         1340 g         1616 t
ORIGIN      
        1 gatatacgac aaggggcaaa ggttatcaga accatgcctc tttttgttgt tccagagtat
       61 gatcgcattt tatacgaagg aaaaacgtac aaagatgtca ccacgagaac tccggcatta
      121 agaaatagca ttatcgtcca ggaggtggct tcaaatgagt aagagaaagg tttttcaaat
      181 caacggatta tctggattgt caaagaaact caaaaaaaac gccacgatgg atgatgttaa
      241 aactgttgta aaagtaaata ctgcggaact cacagaagcg atgcaaagag agacgcaaaa
      301 ggtactcaca ggtcactggg aaggcggcaa attcgtcaaa cctactggtg cgactaagcg
      361 aagcattacg atgaagctct ctaacaaagg actttcagga catacgggac caaaaacaga
      421 gtactctccg tatttaatca gaggaacacg atttatggcc aagcgtgatt ttttcttacc
      481 acctttgaga attcagaagg cgaaatttaa atctgattta gaacggttgg tggaatagat
      541 gaagactaga gaacaatcaa tctttgatga aatgtttaaa cgatcgaatg cattggggta
      601 tcaaacctat gactacaaac ctgcgagtgc tgctagctac ccgtttgtag aatttgaaga
      661 cactcaaaca cttcattccg ctaacaaatc tcatgtcttg ggaaatgttg tgattgtaat
      721 ttccgtatgg ggtttgcata caaagcgaaa acaggtgtct gaaatggcgt ctgctttgtt
      781 tgagcaagcg atgcaagtaa ataaatctga cggatattca tggatgctag acacgaatgc
      841 aagcgacata cagacggtaa cagatacaag cacaaacaca ccgcttaaac gagcgattat
      901 tgaattgaat tttagattaa taggaggaat ttaaatggca ctaaaaaaag gtattgacgt
      961 cattttggtc tatcgggact tggaaaagca agctgaagaa gctgccaaaa cagtgactta
     1021 tcaaactgag catacattcg gtatgtcgcg cagcacagat gcaactgaaa ctaaagatgg
     1081 cactgcacaa actgtagggg caattgagta tgatttcagc tctactgctt tatatgaacg
     1141 cggcagcaaa acactcaaaa tgctttacga tgcatttatg aacaacaagt tagtagaagt
     1201 gtggatcatt gacaaactcg aaccacaaga aggcgataca gggaaatttg cagctaaata
     1261 tatgcaagtg tatatctcaa gctacgaaga aacggcttct gccgaagata acgttgaagt
     1321 ttcattggaa tacgccgtgc aaatgattca tcaggacggc tatgcaacac taacaaccga
     1381 acaacaaaat gcagttcaat atgcatttgt ggacacaaca aaacaaactt aattgagtgc
     1441 ttttattttt aggaggatga ataaatggaa ctaacgatta acaacaaaga gtatagcttt
     1501 atttttggat ttgggtttat ccgtgaaatg aaccgcagat attccgttgt agagcaaggg
     1561 atgacaatga agcttggctt agattcaacg ctagtgaatt tctttaacga agatatcgag
     1621 actttgattg aaatgttaaa agtagcaaat gcaacagagt caccacgagt agcagaaaaa
     1681 gatttgattg ccctagttgg tgaaatcggc tcggataaac tctttgattt agtactagaa
     1741 gaattaaaaa agtcggaatt tacaaagaaa aaaacactaa cagtcgaaaa cagaatcaag
     1801 gaaagcaagt agaagaagat ttttatgcca ctgtccagat aaactgtctg cgttatctcg
     1861 ggattactga ctttttagac attgatcgaa tgacaatgac ggaatacgaa acaaggctta
     1921 ttgcttatcg tctcaaaagg ttagacgaac aagagcttat tcattaccaa gcatgggcga
     1981 ataatcaagt taaagctact aaaaaacgtg gtaaatacga ggttccttta tttgatacct
     2041 tcgaaaaatt ctttaacaag gaaaaacttg aaaacaaaat cttgggcaag gatgaaaaag
     2101 taccgagatt tgtaaactcc tgaggaaagg aggaaaacta tggaatcata tagcgttgaa
     2161 gcgatccttt cggctgtcga caagaatttc acgtctacaa tgaagaatgc tgatagttcg
     2221 atgagtgggc ttaacgacaa tacaaaaaag accaatactt ctatcctcga tatcgccaaa
     2281 ggtatcgggg tttttaaatt agtggattca gcaattgggt tagtcaagag ttcgatggat
     2341 ggcgctatcg atcgtttcga tacattgaac aaatacccag tcgtaatgca agcattaggt
     2401 tattccgctg aagaagtcga caagtcaatg aacaaactga cagacggaat agacggatta
     2461 ccaacgagtt tagatgaaat tgtatctagc gctcaacagc tcgctatttc aacgggtgat
     2521 ttgcaaaaag gaacagacac agccctagcc ttaaacaatg cttttttagc atcaggtgct
     2581 tctacagctg atgcaagccg aggaatgcaa caatatcaac aaatgttagc aaaaggtaca
     2641 gtcgatatgc agtcgtggcg aacattacaa gaaactatgc cgattgcaat ggataaagta
     2701 gcaaagtcat tcaaagatca aggtgttaac tcagtaaatg aactttacga tgccttgcaa
     2761 gatggaacaa ttacatttga cgacttcaat aaccgactta taaagttgaa tgatggtgtt
     2821 ggcggatttg ctgaattggc taagaaaaac tcagccggaa taaggacatc attctcaaat
     2881 ataaaaacag cagttgtaaa aggattggct aatgtcatta ctgcgattga tgaaggaatg
     2941 caaaatgctg gacttggatc aattgctgaa aacttcgaca agattaaaaa tgcagtaaat
     3001 gtggctttta aagctattac tgatagcatt ccaccggcta tcagcttctt aactaatttg
     3061 tgggatacta ttaaaccttt cttaccgcta atcatggcag tagtaggata tataggcatt
     3121 taccaggcag tcatgggtac tgctagaaaa gcagttgagc tatacaacgg cgctcaaaaa
     3181 atgatgaacg tcctaatgaa cttaaaccca atcggactcg ttatagcggc ggttatcgct
     3241 ctcgtagcag gattcattta cctgtggaat accagtgaag aatttagaaa cttttggatt
     3301 ggactgtggg aagcaataaa gacagccacg ggtgcagcag tagactggat tgtatcggcg
     3361 tgggagggca ttaaacaatt ctttgtcgac acttgggaaa gcatcaaaca aggcacaatt
     3421 gacgcttgga acgccttaaa aacatggtta tctgaaacat gggatagcat gaaacaaggt
     3481 gcgattgaca cttggaatag cgtgaagcaa ttcttcattg atttgtggga atctattaag
     3541 acaaacacaa tcaatatgtg gaatgcgatc aaagacggtg tcacgactgc ttgggaaaat
     3601 accaagaacg ctgtcattaa tacagcgaag agcattgtag acggtgcagc acaagcttgg
     3661 gaagacatga aaaccggcgt ttcaaatgcc gtagacagag taaaagaaac ctttgataca
     3721 atcagacaaa ttgacctgtt gcaaatcggg aaagatatca tcgatggatt agtaaatgga
     3781 atcagaagca agattgatga tgtagtgaat gcagtcaaag atgttgctgg ttctattact
     3841 ggaaaaatca aagatgtgct gaatattcac tctccttctc gtgtgatggc tgaattaggt
     3901 atgttcactt cgcaaggttt agcagaaggt atgttagatg gcgctcgcta tgttgatcgc
     3961 gcatcttcgc aaattgcaga ccgtgcatcg aatatggata ttggcaatcg tatatcggca
     4021 gtcaatagcc agattcaaac gcaagtccaa cacgaagtca gctacggaac gaatagcaaa
     4081 ccagctgtat ttaaattcaa tcttggaaga caatcgttta gattgtttat ggacgatatt
     4141 tcacaagcta tgggcgaagg tgcagacatt aatctagaat tttaggaggg aatattttgg
     4201 atcagcgaga aaataaaatg tactcattca aagatacaac cattaatctc actagttcta
     4261 aacgattcct tccgacgtct gccatgatgt acgatggaat gtatttagaa gatttgattg
     4321 atggttatca aacacttaag gtggaaggta gagaaatgct ttctgtagaa gttgaacagc
     4381 aagagataca aattggttca atcattacaa atcagaaaat accttcaaga acactaaaaa
     4441 taacatataa actggaagat agagatccag aaaaactaca gtttaaattc aaagaactgt
     4501 tgaattattt ataccggaat gaagacgtgg aaattaggtt tcatgatgaa ttagattatt
     4561 attactacgg tcgctataca tcaactgata ctgttccagg agactccaac tcgattattt
     4621 cgagttttaa tgtattctgt gcggatccac taaagtatac gaaagaatgc gttagtgatg
     4681 gctatattgg aaatccaata cagtttccta taacaccaag aaaaattgaa gttactttat
     4741 ccatgaataa ttcaatcaaa attacaaacg gagaacaaaa tatcacgata actgatgcgg
     4801 caataaaaac aggagacgtg ttggtttttg atttttccga tgagcaggta actgtaaacg
     4861 gagaagattg tacttctatg attgatttag aaagtgattt tgagaacttt tatctcaagc
     4921 aaggtcagaa gataactagc aataatggga agcttaaaat attctatagg ggggcgacaa
     4981 tttgagtgag acagtttatt tctttgatca cttgcaaaaa cttatcaaaa gaaaaaataa
     5041 aagaagttta attgaagtct cccaagaaaa agaaatcacc tctgacaaaa gcgatttaat
     5101 gaaagacacg ctacaagtta caaccacgta tgataaagaa attgaagaag caagttttat
     5161 ggcggttcgt gaaaacaaat cgtctttttc tttgtatcgg ataacaaaga ttagtgatcc
     5221 agccgagact ttagagttta ctgggatagg atttgcacct aatgaattag atgcttatat
     5281 cattaaagat attagaccta gcggtgaacc gataaaaagc gttcttgagc gtttgattgg
     5341 ctttacagag ggaaactggc gtgtcggtca tgtggatgcg atgttgccag cagtgacagc
     5401 aactttttac tatatatcag ttaaagaagc attgaaacaa ttgcagactt taggaatgga
     5461 aattctattt agatgttcct tagatgctga cggtatcaaa gataaatgga ttgaagtata
     5521 tgaacaaatt ggggaggaat ccaatacacg atttgtatac ggaagcaaag ctttaacagt
     5581 cgtcagagaa atcgaccgta gctcaatttc cacttctatg atcggtagag gacgtggaga
     5641 agaggtagga gatggctatg ggcgaagaat tgaatttaca gatatagaat ggaaaaaagc
     5701 caacggagat cccttagata aacctaaagg acaaaattgg cttgaagatc ctgatgcaac
     5761 acaaaagcat ggtatcccac aaaaagatgg ctcaatgagg aaaagggaaa ctgttgtcgt
     5821 tttcgatgac atcgaagatc ctaaagaatt acttaaaaat acttatgcca cattagtgga
     5881 atctgctaga cctttggttc agtttaaagc tgaggtggtt ggaggagatg tgataggcaa
     5941 tatcgttact atacacagac acgataaaga ctatcactat aagactcgta tttataaaac
     6001 tacttttgat cgtctgacag gaaaaactac tatcgagtta ggggataacc taacaaaaga
     6061 tgctaggaag caaacagcat cgattatcaa taatattaat agtctagaat ctagcaaaat
     6121 gacattttac gaatcgacag agattggaaa atatcaagat gacattatgc gagg
//
LOCUS       DACEOM010000120         5948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEOM010000120 DACEOM010000000
VERSION     DACEOM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.127"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..257)
                     /locus_tag="IW781_002801"
                     /pseudo
     CDS             complement(1..257)
                     /locus_tag="IW781_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            278..1480
                     /locus_tag="IW781_002802"
     CDS             278..1480
                     /locus_tag="IW781_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAQ1183767.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2213..3181
                     /locus_tag="IW781_002803"
     CDS             2213..3181
                     /locus_tag="IW781_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1183768.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3192..4007
                     /locus_tag="IW781_002804"
     CDS             3192..4007
                     /locus_tag="IW781_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ1183769.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4075..4785
                     /locus_tag="IW781_002805"
     CDS             4075..4785
                     /locus_tag="IW781_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1183770.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            4821..>5948
                     /locus_tag="IW781_002806"
     CDS             4821..>5948
                     /locus_tag="IW781_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ1183771.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2038 a          896 c         1206 g         1808 t
ORIGIN      
        1 aagctgcgat tgatgtgttg gacaatatga aagcggtcag ttacgatctc cgcatttgga
       61 aagaccgttt taagcaactg acaatagctg gcattcatgt ccatcactag gtacttgacc
      121 tttaaacggg cctttctagg aaagcgcagg aagtattgag ttagtttgta aagtctgcga
      181 tcttcaaggg tcgtaaatag ctgattggtt gtcccatcaa cacaaataaa actcatggaa
      241 ccagaagtgg caacattgtg tacttttctt tgcaatcttg tgtacaaaac tattgcaaaa
      301 cacagcgcat aagaatcctt acaaaacagt agatgaagca acccaattag atcttgttat
      361 agttgttaat cagctcttaa cgggttacga ttcaaaatgg gtcaacaccc tatacttaga
      421 taagtcactt gaatatcaga acataattca ggctttttca cgcacaaatc gtcttaatgg
      481 tcctgaaaaa ccgttcggaa ccattcgtta ttatcgtaaa gcacatacga tggaacagaa
      541 cattgaagag gctattgagt tatactcagg caaacaagca aaagggttat ttgttgaaaa
      601 attatataat aacttagaaa agttgaacga tatattcgaa gaaattaagt ccgtatttaa
      661 tatttctggc atagaaaact tcagtcattt accggaggtt ccaggggaaa ttaagcaatt
      721 tgtaaatctt tataatcaac taatccattg ttatgaatca gcgatgatac agggattcac
      781 atgggcaaaa tcgacttatg aaatccagga ttatgaacct ataacagtag taatcacaga
      841 atatgaattt aatatcttgg cacaacgtta cagcgagata gagagagaac ctagagacgg
      901 aaatgatagt atagcgtatg acattgatta ccaattacct gaaggtagtc gtagtcgtat
      961 taattatgag tatttaaaca caaacttcaa aaaatacatg aatataaaag aagaacgtga
     1021 ttcgaattta attcaatcat tgttaaacga cttacatcgc acatttgcgc aattaccagt
     1081 ggagttacag ccatatgctg agatgattct aaatgatgta aataatgggg aattagttat
     1141 caaagaagga tgggaattta ctgattacct caatgaatat cagcttagtg aagaggacga
     1201 ttttatccaa gatttagtgg attcaaccaa tattaatgag gcattattac gtgagatgtt
     1261 ggatttacgc ttaaccgagg taaacattaa tgaatatagt cgatttgata aattaaaatc
     1321 aagtgttaac gtaggtcatt tcagtggata tatcgagaaa aatttaggta aaatggtaat
     1381 accaatcaag gtaaatatgc taattgacag attattgcgt gcatttttat tagaagatgt
     1441 attcgatgtt acagagtaca taaagaatta ctttaactag agtacaccta catcttatag
     1501 tacataaaaa aattcatctt ttttagttta ataattcacc aaaaaggtga gtaatgccat
     1561 ttttgaaaat tgagcttgtt ttatgtctac atctgtatcc gtatataatc caaaggatta
     1621 taaactcaga agagtcttta ttttttctga atataactac tgtatcaaaa acaatcgatt
     1681 atgagacaaa acaaaatgac ctcttttatt taagaaagga ggtcattttt tgtatcataa
     1741 attgtattaa aatcaatgta tcacttttta atcaatttta agaccataaa ttgcgtacta
     1801 aaagaaattc tgcaattcaa aataaagcat tttaagccat tttcaaattt aagtgcaccc
     1861 atctacccgt caaatcactt tattttccac tccagtagtt tcctaattca actgtgtgca
     1921 aaacgatttc ttctaaacaa atcaagacaa acttataaac cttcatccca gcaccataat
     1981 tatttaaatc ataggcattt ttcgaaatta gtatatgtac tcaagtttca ctgcagatta
     2041 tttatatttt ttagagaaat aaatttttcc tattgacaaa cacaaagaaa gcggatacaa
     2101 tacaattatg tcacatgacg ttatatgacg ttatgtaata tctttacatt taatcagtta
     2161 cttttatttt ggataaaatt aacattttta aaattttaag gaggaaatgt tgatggatgt
     2221 agcaaaaacg gttaacgcaa ttgtagaaaa aaatgatgga gagatttctg gaatttattt
     2281 ggtggcttgt ggcggttcgc tggtagatat gtatgtctca gattattttc ttaaagccga
     2341 agcatcgaag tgtattacag gcttatatac agcgaacgaa tttaaccatg taaccccaaa
     2401 gcaattagat gataaatctg ttgtcattct atgttctcat ggaggaaata cagcagaaac
     2461 ggtaaaagca ggagaagtcg ctcaagctag aggagctgtg actgttggtc tgactcataa
     2521 taaaactgcg gatttattaa aggtttctga tcatagtttc ctttacgaat ggggagatga
     2581 tgcaaaaata gtcaataatc ctatggccat cattcttgat ttaactgtat ctttggtcaa
     2641 tgttaccgaa acatttgaac attacggtaa attcagagaa ggcatgaagg ttatcgatac
     2701 cgtaattgaa aatggcatta agcaggtgca ggatcgtatt aaagtgtttg ctgataagta
     2761 caaagatgaa acaatgtttt atatcttagg tagtggacca tcatatggac atgcttatgg
     2821 tttttcaatt tgctctttga tggaaatgca atggcttgat tcaacttctg ttcattctgg
     2881 agaattcttc catggacctt ttgaagtaac agacaaagat acgaatttta ttctcttgat
     2941 gagcattgga cgcactcgtg ctttagatga acgggcaaaa cgattccttg ataaatatgc
     3001 agaaagagta gaaatcgttg atgcaaagga actaggattg gatatgatat cagatgaagt
     3061 ttctgaattc ttcaatccca ttttattcta cagtattctt tcagaatatc gttcagcttt
     3121 agcagatgca cgcaatcatg atttagatgt tcgtcgctat atggggaaag tggagtacta
     3181 ggaggattaa aatgaaggta ttaggcttag gtgataacgt cgtcgataaa tatgaaaatc
     3241 tgggaattat gtatcctgga ggaaattcat tgaattttgc agtttttgca aaaaaactgg
     3301 gacatgaaag tagctttatg ggagtttttg gctcagatga ggaggcagct catgttttat
     3361 cagcaataaa aaaaatagga ctggacaaca gtcactctcg ctttgaaaaa ggcgagaatg
     3421 gctgtgccag agtgaagatt gatgagggcg accggatttt ccttggcagc aattcgggag
     3481 gtgttactcg tgaatacccg attcagctta ctgaagaaga cagagaatat ttaaaccagt
     3541 ttgaattgat tcatatggga ttatatagcc atgtcaatca tctattagaa gagttacaaa
     3601 atgtttctgg aaagatttct tatgattttt ctgacgactt tacggaagaa gaggttcagc
     3661 gggcaattga taaagttgat tttggcttct tttctataga agacttttcc gatgaagaag
     3721 taaagcagtt tttgatgaag catttttgtg agcgaaatga tgtattgatt gcaacccgag
     3781 gagaaaaaac ggctattggt tatgatggag caaagtttta tgaagtgaac cccgtgctag
     3841 aagaaccagt tgatacaatg gcagcgggag attctttctt aacagctttc ctactttatt
     3901 ttctcgaagg ggacgatata gtcaccgcga tgaaaaaggg gaatgaattt gctgggaagt
     3961 cttgtctggt tgacggttcg ttcggctttg gtattcatta cgattgagct tgttttaaat
     4021 tcctgcattt gataacctaa agaaagaaat cccggtaaag tggaggttaa aaaaatgctg
     4081 gatacaaatg cacaattacc attgtactta caattaaaag aaatattaaa aaataaaatc
     4141 aatacagggg agtatcccga gaatacgaaa attcccgctg aatcagagct gatagaaagt
     4201 tatgaggtca gccgaatcac agtgagaaaa gcagtagaag aactggtaga agaagggtac
     4261 ctacttaaaa aacaagggaa aggaactttt gtcaaccggc ataaagtttt tcgtaaaatt
     4321 gaatatgtta tgggtttcag tgagagttgt aaatctaatg gttttacacc gactagcatt
     4381 gttttggaac gcaagatcat tccagcaact gaagggatgg caaaaaaact cgatatcgct
     4441 caaggtgatg atgttttata tatccagaga aagcgaatgg cggataacat ccccattatg
     4501 ttagaaaaca attatttttc ttatgaaaga tatagtttct taatggagga agagctgact
     4561 gattctttgt atgaagtact taaagtccaa ggtaccttag ccatcaatcc tggggaaact
     4621 gcacttgaaa ttgtcacagc tgatgaccaa attgccaaaa taatggaagt agctgttggg
     4681 acgccctttt tctttatgga tactttaatc aacgatcaaa acaacatacc aattcatgta
     4741 ggtaatcaat attacctggg agaagtatat cgtttttact tatgatttcc tgttttgatt
     4801 tcaaggagta gattttattt atgaagcaag tattgcggtc aaaaatgatt tttgatggag
     4861 agcagccacc tttcaaaggt tatattgtaa tcaatgaagg gatcattgaa aaggtggaaa
     4921 acaactggaa ttacagtgat ctattaaatg aaaacacgga attaattgct tatgatgatc
     4981 aattcgttat gccagcaatt catgataatc atgtattttt cagtggttat ttggctatga
     5041 atgctggcat agatttaagc gatacatctt cagctaacga ggctgtagag cgtattaaag
     5101 ctgtaatgaa ccataaaaaa gcagatgaac caatttatgc tcatggctgg gatgctgccc
     5161 tgtggaaaac tgacccatca gaagagctat tgaatgaaat aaaccacttt ggtccaatca
     5221 cagcgatcga caaaaatcgc agccattgtt ggatgaatgc tattgctaag cagacttatg
     5281 gttttacaga aaaagaaacc agtgctgaaa gtcgagtatt attaattaag gaaatgctag
     5341 caaacaaaaa gcttttagca gaggtttacg ctgaatttga agcattactg ctttcaaaag
     5401 gtgttatatc aatgaaagaa atcgtttttg atgacgcaga atttctttct aatatacctc
     5461 agaggaaaat acttagtaac ctatatgtgc aggcagtcca agaacctcta cagcatgaac
     5521 ttctatatga gtataaggaa aaagttttcc ctgaaaaggt ccgctttggt ggagtgaaga
     5581 tcatggtaga tggtgttgtt gcggatagaa gtggagatat ttacggtgtt tatcagtcag
     5641 gagtagcttc acccgagatt gactatgagg caattaaagc agaagttgct gcattcaatg
     5701 aagtagagat tccttgctgt ttgacaacgg aaggagataa agcgggttat aaagctgcgt
     5761 caattcttgt cgaatttgga aagcggttac ctgaaggcgt ttttaattcg attagtgatt
     5821 tggaaatggt tactcaaaag acagcagaaa aaatgaacga aggacaagta gtagcagagg
     5881 tatatccaca aattctcggc ttgaatcaaa cgttagaaga ggcttacatg tcaactgtta
     5941 taaaaaat
//
LOCUS       DACEOM010000121         5874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEOM010000121 DACEOM010000000
VERSION     DACEOM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.128"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            641..1375
                     /locus_tag="IW781_002807"
     CDS             641..1375
                     /locus_tag="IW781_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1183772.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IW781_002808"
     CDS             1391..2560
                     /locus_tag="IW781_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1183773.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IW781_002809"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IW781_002809"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1183774.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IW781_002810"
     CDS             3650..4576
                     /locus_tag="IW781_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1183775.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IW781_002811"
     CDS             4595..5845
                     /locus_tag="IW781_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1183776.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1966 a          955 c         1203 g         1750 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DACEOM010000122         5733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEOM010000122 DACEOM010000000
VERSION     DACEOM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.129"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..121)
                     /locus_tag="IW781_002812"
     CDS             complement(<1..121)
                     /locus_tag="IW781_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1183777.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASA"
     gene            complement(162..416)
                     /locus_tag="IW781_002813"
     CDS             complement(162..416)
                     /locus_tag="IW781_002813"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1183778.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(527..781)
                     /locus_tag="IW781_002814"
     CDS             complement(527..781)
                     /locus_tag="IW781_002814"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1183779.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(974..1249)
                     /locus_tag="IW781_002815"
     CDS             complement(974..1249)
                     /locus_tag="IW781_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ1183780.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1221..2174)
                     /locus_tag="IW781_002816"
     CDS             complement(1221..2174)
                     /locus_tag="IW781_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1183781.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2786..4279
                     /locus_tag="IW781_002817"
     CDS             2786..4279
                     /locus_tag="IW781_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ1183782.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4414..4710
                     /locus_tag="IW781_002818"
     CDS             4414..4710
                     /locus_tag="IW781_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1183783.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4734..5693)
                     /locus_tag="IW781_002819"
     CDS             complement(4734..5693)
                     /locus_tag="IW781_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1183784.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1828 a          979 c          997 g         1929 t
ORIGIN      
        1 atgctgaagc cgttagttca tattctcgaa tgatttctac acgtggcttt ccagctaagt
       61 aaagattgac gatttgttgc ttgaattctt gtgaaaaagt tcttcgtgtt ctcttagaca
      121 taaaaattcc tcctggtatg ttttcttcta gtctacacac cttaattttt ctgtctagtt
      181 aattgtagcc tatccatgat cggtttatta gacctttgag gtctatattt tttaactaca
      241 atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga tactgaaatc
      301 ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt attcgtaaaa
      361 aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc tgtcaacggt
      421 ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca caacaaagaa
      481 acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag gttttccgag
      541 agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga gcattttctt
      601 ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt aaatataaat
      661 tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac cctcgaacag
      721 aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca tatcgggtca
      781 taaaaattca tcctctcgta gataagttta atttattctt gtctctacga aaagctgtct
      841 aagatttgat actaacacca aatgttgtat acccccaaac aatacacata ttttgaagga
      901 ttaggattaa taaagctaaa aatatagtaa gtatactaaa tatatttact atatctagta
      961 tacttactat attttaatct tttgatcgtc tattatatat aatccgttcc tcgtcgttta
     1021 aagtatcaac ataggcgtcc attgcaagtt tgactatttc atacatagga atatcttttg
     1081 cataactaag atcccgaatg gtttttagta cgggaggatc tacttgaact ggttttcttt
     1141 ctttcgcagt gtactttcgt tcccctaact cgacattaat atcatcttta attgaaatat
     1201 cttcagcacc aattttcttc ttatctttgt tcgttggtaa taatgccatt tagtttaatc
     1261 tcctttccaa gaggtaggat atgattggcc attgagtcag catatttagg tgtataatca
     1321 aatccttcaa tatcaccatg ttgttcatag tattcaattc tttcttctaa ttctgtaaag
     1381 atgtctgcaa aaatagccca ccatcttcga tctacataat catttaattg gataccattc
     1441 tcaccataca tttctaaccg atccgagcct ttaacaatgc ttttaaaaat gtttgattca
     1501 ccatacttat caattgtttt ttgatagttt gcgtgttgag aggaacgtcg cactgagaaa
     1561 agaacaggca aaattccaat gatttgaaga tgagactctt caaaattcgt gacaatcgga
     1621 actaaaactt tattaataaa atcttctgtt ccttccattg cgtaccgttt taattcttgc
     1681 attgcaataa tataatctgc agcagttaag aatgcacgaa ccacattatc tgttgatggt
     1741 gggcagtcga taaagatata atcgaaattt tttctcaatt cttctaaagg tttaatgaag
     1801 gccaatgatc tcttacgacg atcaggatat ttttcggaca agtattcatt aagttcttca
     1861 gatccaggag agccagccat aatatataaa tgtggactta cttgagtaat acctgaaacc
     1921 cagtcccctt ttttgattgc tgcagacatg gatcgatcaa agctagtaat ttgatacgtt
     1981 gatccaatgt tggaagtagt atttctttgt tggtcataat caattagcaa cacgtctttt
     2041 tcaaaaagtt gactagaaac aatcgctaat aaatcattgt ctgtagtctt accaatgccg
     2101 ccttttctac ctgtaagcat tacaactttt cctagtttat ctgatgtttt aaatgcagtc
     2161 cgtaagtttt ccatagttcc ctccaatttc ctatactaaa tatatttagt atacttcata
     2221 tacttaatat attcgatata tctaatatac tccaaattga taagaattca gactttataa
     2281 ggaatatagt ggatatacta aatatatttg ctatattcag tatatcagat atattcaaaa
     2341 tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga gtgaaaagag gtaaacttaa
     2401 ttctatagaa accacagata attcactatt tacaaagtag caaaagcagt ggtactcttt
     2461 aaacagaaaa attacataaa aaaaagagac ggaaataatt gattttagca gaatcaatta
     2521 tttctgaatc acatggttag gacatgtgtt cacgctcgtc tcaagaatat gtaagtagct
     2581 accaactact tatattaaaa gttgatcgga taccttttcc gattgccttt attctaccaa
     2641 aaaaaggcga gcaactcaac agaagaacgt ttatttagtg tacttaaatt aaatattctt
     2701 aaacactgaa gagagaacac atactatggc tgagattaaa gctaaactgt gttctctttt
     2761 tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt tattcagcta ttcttaaaaa
     2821 cggcttacgt acatacaaat atcgaaacag caccatgaaa cctgcaggtc atattgattc
     2881 cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa ttaatgaatg ttgggcgtgg
     2941 tatggtcatt acttcggaag aagctatttt agagaacgaa aagaaactaa cgcattggac
     3001 acctaatacc tatcgctttg gtacttatgc ggatgattat cgaacagtgg ttaaaggtca
     3061 ttctgagaaa aacttaagtc aaatcaacac atttgtagtt gatatcgatt caaaagagaa
     3121 ccatcaaggc gaaattattt tagcctgttt agatcaagtg ggctacatgc caacgctaat
     3181 tttagaatca gatcatggtt atcaagttta ttttgtacta aagacaccag gctatgtcac
     3241 aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa atttcaaaaa caattcgtct
     3301 acagttggct gaaaaattgt ccggagtgga tctaggatgt aaccacttcg gaattgctcg
     3361 attcccacaa gaaaataata ttgtttttaa agaaatgaac tatcagtatt cttttgctga
     3421 ctggattcaa tggtcgatga agcttgaatc agatcaaaaa acaaaagaag atcgagaacg
     3481 aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa gtagatgaac cgtggttcga
     3541 tatgttactt cataaagcaa acatcgtcgg cggagaagga cgtcttggtc gaaataatgt
     3601 gattttcacg ctttctttag cttattactc ttcaggttat gcattggata cttgcgaata
     3661 caatatgttc acattcaacg aacggttaca ggaaccgcta tctgaagcag aattagtaaa
     3721 gattgtacgt agtgcttatt ctggtaatta tcaagcagca agcagagaat tcattattga
     3781 attgtgccag caatgggttt caccaacctt aaaaaataat gatctgttca ttcaacgacg
     3841 tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa tatagtcata aaaatgaatg
     3901 gaaagaagat ctattggctt atctaagcga aaaaagtctt gctagtgagc cttatttgaa
     3961 cctgtcaaaa aaagaattgg cttcacaact aacgatgccc aagcgaacac ttgataagat
     4021 cttaaatgag ttacgagagg agaataaagt cttctacaag gtaacacgag gaagaaatgg
     4081 cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg gttattcaac taagtaaaga
     4141 aaagagacta gattattcaa caaggatcac agaggtattt ggactcaacg agagcttagt
     4201 agaagagaca tttagacaat tatcaacccc cttgaaccct cttaaagaag tgaatctttt
     4261 tgaagtagat acaggttgaa aaataaaagg tgcaaattgc cttatacaat atatctatga
     4321 tacttctgta ggtgttgctc cctttgccta gctacgtgta ggtggtatac ttataactgc
     4381 gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt taaaaaatta tgtctattca
     4441 catcctgtta atgtgtttat cattggtcgt ttgggattat ctgtggaagt gttctgtgag
     4501 ctttatggtt ttaagcaggg gactttgtct tcttgggtta ccagggaaaa aacagttgct
     4561 tctttgccga ttgagtttct tcatgggttg tccttagctt ctggggagac tatggatcag
     4621 gtatatgatt gtttgtgtgt gttagagcag gagtatattg agtataaaat agctaacgag
     4681 ttaagaaaaa ggaagaagta tatccagtag tagtttttat ttactcttaa ttgtcaaatt
     4741 aagttagaca aatcatcaat acttacgtaa ctcaaaaata cttccaaagg tgttcgataa
     4801 ttcagtgatt ttctaggaat attatttcgt ttcgatgcga cagattgaat aaaagattca
     4861 tctactgaat tgaaatccat agatttcaat aaaccatctc tacgtaacaa tccgttagaa
     4921 ttctcgttta ggcctctttg agacggcgtt cctggatcag cgaaataaat ggcaatatca
     4981 ttggcattac tgatctgttt ccaatttgaa aattcctttc cacaatcaaa agtgatggat
     5041 ttgaatagat ttttcggtac agattcaaac caatgattta atttgttttc aatatcaatc
     5101 gcttgtctac cacacggttt caatgtgatg ataacttttg ataatcgctc aactaaggta
     5161 attacagcac ttttatgttt caaacctacg atagtgtcac cttcaaggtg accaaactca
     5221 tttgagaatt ggctataatc tttttcacgt tcatgaatag agcgacggaa agcttgtttt
     5281 ccacgtcttt cttgatgtcc atttggttta cgcttgcctt tcatcggtaa gtcagaagga
     5341 ttaaatagcc cctttttgaa catacgataa atggtacgag cagaacaacg aataggaaac
     5401 gctgcacgac caacaatcac atcgggtgtc catccttgaa caacctttct ttgaatatat
     5461 tctgattgtt cctcaggtaa aacaagcggg cgtctaccac agtttgattt gtttttctta
     5521 tactgttgat agtactctaa ggctgatttt ccttgcttga agaacaggta tactttatgg
     5581 atcgtttgtc ttgaacgatt caagaaatgc gcagttttcg caacagattg atttattttg
     5641 aaataagact ctatcattac aagttcatcc gttgtaagat gggtataggt catttgtact
     5701 cactccttat aattctttgg acggaactat ttt
//
LOCUS       DACEOM010000123         5665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEOM010000123 DACEOM010000000
VERSION     DACEOM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.130"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(72..542)
                     /locus_tag="IW781_002820"
     CDS             complement(72..542)
                     /locus_tag="IW781_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1183785.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IW781_002821"
     CDS             872..2512
                     /locus_tag="IW781_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1183786.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IW781_002822"
     CDS             2666..3988
                     /locus_tag="IW781_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1183787.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="IW781_002823"
     CDS             complement(4056..4490)
                     /locus_tag="IW781_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183788.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5391)
                     /locus_tag="IW781_002824"
     CDS             complement(4555..5391)
                     /locus_tag="IW781_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1183789.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1696 a         1128 c         1038 g         1803 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     4981 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5041 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5101 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5161 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5221 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5281 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5341 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5401 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5461 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5521 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5581 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5641 agaaagaggt cgtgagcctg ccgtt
//
LOCUS       DACEOM010000124         5649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEOM010000124 DACEOM010000000
VERSION     DACEOM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.131"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..251)
                     /locus_tag="IW781_002825"
     CDS             complement(<1..251)
                     /locus_tag="IW781_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1183790.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNL"
     gene            complement(238..345)
                     /locus_tag="IW781_002826"
     CDS             complement(238..345)
                     /locus_tag="IW781_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183791.1"
                     /translation="MERCPRCGSEVRETSWSYCTFCGLPLKEEKNNGNK"
     gene            complement(345..1286)
                     /locus_tag="IW781_002827"
     CDS             complement(345..1286)
                     /locus_tag="IW781_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1183792.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVIEESENEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     DVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQRF
                     IWKRIARDQELIDTITERLVEFWETNVVEGIEPVIDGSEATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESAIRQVDNEIISELGKRAASIGITQRNIISWKLVS
                     TKRMNTKKLAEKYPDIASDEEIYSITESRRLTEKEIK"
     gene            complement(1423..1608)
                     /locus_tag="IW781_002828"
     CDS             complement(1423..1608)
                     /locus_tag="IW781_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183793.1"
                     /translation="MKTVFKMTVKSALLMILVAIVLASINPAYALIYWGTLVAVTAVR
                     ESFKMPTQKRPTSDGNR"
     gene            complement(1605..1883)
                     /locus_tag="IW781_002829"
     CDS             complement(1605..1883)
                     /locus_tag="IW781_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1183794.1"
                     /translation="METLENIFPKKVVLKRNNKRNIEKLTYSVTEAALAITTNPQNVK
                     DLIEMGYIGFLKLGEIRIPKTEVARFLENHMNEDLASEIAKYREERKK"
     gene            complement(1889..2434)
                     /locus_tag="IW781_002830"
     CDS             complement(1889..2434)
                     /locus_tag="IW781_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183795.1"
                     /translation="MFKLLKKSSVIRESLVEVINKSGETKKEIARQINVSQQSLSDWT
                     TLLNTKPVTLENAQALTDHFRDSDFTLQVIHEFFGLFKSIDGDVYRRDPSSLDKLQMI
                     ESDERKQKKQEVEKILLKQVNYLTVDDRQQIIAYAYEFLDEIMVEVTLISALCEILGI
                     DIRKLSEERLSYWVAQGYMKG"
     gene            complement(2647..2880)
                     /locus_tag="IW781_002831"
     CDS             complement(2647..2880)
                     /locus_tag="IW781_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183796.1"
                     /translation="MENKMGQIRSRKGISQSQLASLLKVSQKTISSWEVGRTLPKPSQ
                     MQHLEDIFHVPKEKIFFMAFNYKNELKTRGASK"
     gene            3035..3406
                     /locus_tag="IW781_002832"
     CDS             3035..3406
                     /locus_tag="IW781_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183797.1"
                     /translation="MLKDRIKELRKQHGWTQAELAKKMSVSQQTIGSWEVGRAEPNSE
                     ALTKLAHLFNVSTDYLLSNHKTPEWATKEDIIELDKMLDSNVNMAYGGETLTDEEKQR
                     VKDVLTGLFWEFRKEDKSKEK"
     gene            3413..3841
                     /locus_tag="IW781_002833"
     CDS             3413..3841
                     /locus_tag="IW781_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1183798.1"
                     /translation="MEMDVISLVSKLKQKYNSANPFTICEKMDIQIRYVPFLNNPKGQ
                     FQELLGRSVILLNHELKYSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPRKVEELTNLYGLPENVYRFLI"
     gene            3906..4472
                     /locus_tag="IW781_002834"
     CDS             3906..4472
                     /locus_tag="IW781_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HAQ1183799.1"
                     /translation="MKKIVGLGLILFSSITLGACGNNDSNSDVPKKTTINTSTMVTLE
                     SSNSMDSSTEEKSTSDTTFEDDSSKIVIKNTEELSSQYDPNKKILAIEIQYTNKSDKP
                     QSPWMAFATSIKPIQETDKTEELLNGANGLFPQDYKPDLVKMGDTDVKPDATVDAVVG
                     VEIIYPGSPIIMKDFMDNGSFEKTIPTN"
     gene            4595..5425
                     /locus_tag="IW781_002835"
     CDS             4595..5425
                     /locus_tag="IW781_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183800.1"
                     /translation="MSTFLILVGFFGFLFGVCFLVYSFFSKKKRSKKKISIGILVAFI
                     VMVIGGALAPPTTGQADVHKDAKSSSSSVSVSSESREKDEKKAKELAKKKKAEEQKAK
                     EEAEKKHQEEQKAKEAEKKRQEEINQKTSTAKTTLEQAEANPTRDNYNAALSAIQSIP
                     GGNQELLNRLVNVDSTIKSNEAAEAEQQKQQAAEAQRQAQEQQAAEAQRQAEQQNNSY
                     TVDGQWSIAANGMVFARSDSGKYYSRVTNPNNYQYMTQIDADNAGYSRAPRGNQYARP
                     "
BASE COUNT         1746 a         1069 c          948 g         1886 t
ORIGIN      
        1 agatttaatg ttgcagcagt taacgcacca ctcatgatag acatcggttc actatctgca
       61 agataactgt cattagatac aagagtcata acattcgaca taaaagcatt agcattgtca
      121 tgaagtactt cttcaaattt ctttctcatt gttggtgtat tcattagagc tttaagccct
      181 aactgtcctg gtgcaacttg tttctgtggc ttttctgcca attggttttt taacgattca
      241 tttgttgcca ttgttttttt cctccttcag tggtaatcca caaaacgtac aataactcca
      301 agatgtttct ctaacttcgc ttccacagcg gggacatctt tccatcattt aatctccttt
      361 tctgtcaatc ttctagattc agtaatgcta tagatctctt catcacttgc gatatctgga
      421 tatttctctg ctagtttctt cgtgttcata cgtttagtac taacaagttt ccagctgatg
      481 atgttccttt gtgtaatacc aatacttgcc gcacgttttc ctagctcgct gataatctcg
      541 ttgtctactt gacggatagc tgactcaatt tcttttttcg tccgcttgag ttctcttttt
      601 tgctcgataa gttcatcaaa acgcgatggt agagctgttt gattttcttc tacatctgca
      661 tatttttctt ttaagaagtc agcagtcgct tcacttccgt caattacagg ctcgatacct
      721 tcaactacat ttgtttccca aaattcaacc aagcgttctg taattgtatc gattaattct
      781 tgatctctcg caattcgctt ccaaatgaat ctttgtccgc caatcaacac agcgatataa
      841 caataatctt tgttcaaaac attcatataa tgttgaacct gacagagata gctaagcggg
      901 acttcttctc cttcccactc tttaccaaga aattggttag ctgttttgca ttcaagaatg
      961 gcgttttccc ctactacgtc acgatcaata tttgctctta aaaatggatg taacggatgt
     1021 tcaaacactt ggtttcttct gcgtactttt ttgcctgttc gttcttgaaa ttctttggca
     1081 acaacttctt ctaagacatt gccccaataa gctggttcat tttctgattc ttcaattacg
     1141 acttgtcctg ttttttctag ccagagttga taaggtgatt tccacttatt caatcctaaa
     1201 atcgttccga catcagaacc tccgatacct ttcttacggt cttcgagcca ttcttgatgg
     1261 ctcatttcta aggtagattt actcatcgtc ttccacctct tgatgtgccg tgccccattc
     1321 aggagtcgtt aaatattgat caagcgcttg tccaaaatca ttcattgttt tagccttcct
     1381 ttcgtgctaa aatacagtta agttattttg ttatgttgcc gattagcgat tgccgtcgct
     1441 ggtcggtctt ttttgtgttg gcattttgaa actttctctt acagcagtaa ctgctactaa
     1501 ggttccccaa taaataagtg catatgctgg attaatactt gccagtacga ttgctactag
     1561 aatcataagc aaagcgctct tgacagtcat tttaaataca gttttcattt ctttctctcc
     1621 tctctatatt tagcaatttc gctagcaaga tcttcattca tatgattctc taaaaatcga
     1681 gcgacttcag ttttaggaat tctaatttca ccgagtttca aaaaaccgat gtatcccatc
     1741 tcaatcaaat ctttaacatt ttgaggattt gttgttatag ctaatgccgc ttcagtaact
     1801 gagtatgtta atttttcaat gtttctttta ttgttgcgct tcaagacaac tttttttgga
     1861 aaaatatttt ccaatgtttc catttccatc atcctttcat atatccttgt gctacccagt
     1921 acgacagccg ttcctcacta agcttgcgaa tatcgattcc aagtatttcg cataatgcac
     1981 ttattagtgt tacttccacc atgatttcat ctaaaaattc ataagcatat gcaatgattt
     2041 gttgacgatc atcaacagtt aagtaattta cttgtttaag aagaattttc tctacttctt
     2101 gcttcttctg tttccgctca tctgattcaa tcatttgcaa cttgtctaat gatgaaggat
     2161 ctctcctata aacatcacca tctattgatt taaataaacc aaagaactca tgaatcactt
     2221 gaagagtgaa atctgaatct ctaaaatgat ccgttaacgc ctgagcattt tccaacgtca
     2281 cgggcttcgt attaagcaat gttgtccaat cgcttaatga ctgttgagag acgttgattt
     2341 gtcttgctat ttccttttta gtctcaccac tcttattaat tacttcgact aacgattctc
     2401 gaataacact tgattttttt aacagtttaa acacctcata ttcttattcg cccgtatatc
     2461 aatacgagca atttttttat actattaatt taaagaatca aacgaaagct gcttcatcta
     2521 gttcacgttc aagctctttt tgaacttctt caactaaacg atcaagttga tcatctgtag
     2581 cacacttgat gatgtgaact agtctaggtc tagcatcaag tacgatgttt attttttctt
     2641 gtcgtgtcat ttggaagccc cccttgtttt taactcattt ttgtagttaa aagccatgaa
     2701 aaaaattttt tctttaggaa catgaaaaat atcttctaga tgttgcatct gggaaggctt
     2761 aggcaaggta cgtcctacct cccaagaact gattgttttt tgggatactt ttaataaaga
     2821 cgctagttga gattgagaga tcccttttct agatcttatt tgtcccattt tgttttccat
     2881 acatttccac ctctctttta ctaccttatg tagttataat atactacaaa atttagtaag
     2941 tcaaccacta ttactacttt tttttgtatt aactaaattt cgttgtaccg actacgcttt
     3001 gtagtatata attttattaa aaggaggact tgctgtgtta aaagatcgaa tcaaagagtt
     3061 aagaaagcaa catggctgga cccaagcaga actagcaaaa aaaatgagcg tgtcccaaca
     3121 aactatagga agttgggaag taggtcgtgc tgaacctaat tcagaagcac taactaaatt
     3181 agctcatcta tttaacgtta gtactgacta tctattaagt aatcataaaa ctccagaatg
     3241 ggcgactaaa gaagatataa ttgaattaga taaaatgctc gattccaacg ttaacatggc
     3301 ttatggtggt gaaacattaa cagatgaaga gaaacaacgg gtaaaagatg ttttgacagg
     3361 tctattttgg gaatttagaa aagaagacaa aagtaaagag aagtgatttt ctatggagat
     3421 ggacgtaatt agtctagtta gcaaactgaa gcaaaaatat aattcagcta atccctttac
     3481 tatttgcgaa aaaatggata ttcagattag gtatgttcct tttttgaata atccaaaggg
     3541 acaatttcaa gaactgttag ggcgttcggt tattcttcta aatcacgaac taaagtattc
     3601 tgaagaacgg ttctatattt gtgctcacga actaggtcac gccatctttc atcgtgggtt
     3661 atctagctat tatgtatcta cacgaacatc cagaagcaaa tcagaaagcg aagcgaattg
     3721 ctttgctgct aatctcattg tttctcttta taaagaagac aacgatcaat accctagaaa
     3781 agttgaggaa ttaacaaatt tgtatgggct acctgaaaac gtgtacagat ttttaattta
     3841 aaattggcga ctatcactac ctgccattaa gtgggagtaa attattttat tttttggagg
     3901 aaaatatgaa aaaaatagtt gggttaggat taatcttgtt ttctagtatc acattaggtg
     3961 cttgtggtaa caacgattca aactctgatg tgcctaaaaa aacgactatt aatacttcca
     4021 caatggttac tttagagagc agcaactcta tggattcaag tactgaagaa aaatctacaa
     4081 gtgatacaac ttttgaagac gattcatcaa agattgtaat aaaaaacact gaagaattat
     4141 ctagccaata tgatccaaat aaaaaaatat tagctattga aattcaatat actaataaat
     4201 ctgacaaacc tcaaagtcct tggatggcat tcgctacatc aatcaaacct atacaggaaa
     4261 cagataaaac cgaagaacta ttgaatggag caaatggctt gttccctcaa gactacaaac
     4321 cagatcttgt aaagatgggc gatacagatg ttaagcctga tgctactgtt gatgcagttg
     4381 ttggtgtaga aattatttat ccaggttctc ctattatcat gaaagatttt atggataatg
     4441 gaagttttga aaaaactatc cctactaatt aaaaaaggtt ggccttccgg ctttcttttt
     4501 gttaaggctt tattaagggc gactatcact acctgccttc aagtgggagt aaatacttga
     4561 tttacccagt gatatattca acaaggagga ataaatgagt acgtttttaa ttttagttgg
     4621 gttctttggt tttctatttg gagtatgttt tttagtatat tcttttttct ccaagaagaa
     4681 acgttcaaag aaaaagatta gtattggaat tctagtagct tttatagtta tggtaatcgg
     4741 aggggctctt gctccaccaa caactggaca agcagatgta cacaaagacg ctaaatcttc
     4801 atcttctagt gtatctgtct cttctgaaag ccgagaaaaa gatgaaaaaa aggctaaaga
     4861 attagctaaa aagaaaaagg ccgaagaaca aaaagctaaa gaagaggctg aaaaaaagca
     4921 ccaagaagaa caaaaagcta aagaagctga gaaaaaacgc caagaagaaa ttaatcaaaa
     4981 aacttcaaca gctaaaacca ctctggaaca agctgaagct aatccaacta gagataacta
     5041 caatgcagca ttatcagcta ttcaatctat acctggtgga aaccaagaat tattaaatcg
     5101 cttagtcaat gttgattcaa ctataaaatc taatgaggca gctgaagcag aacaacaaaa
     5161 gcaacaagct gctgaagctc agcgtcaggc tcaagaacag caagctgctg aagctcaacg
     5221 tcaagctgaa caacaaaata attcatacac tgttgatggc cagtggtcta tcgctgcaaa
     5281 tggtatggtt ttcgctcgtt cagatagtgg aaagtactat tctcgtgtta caaatccaaa
     5341 taattatcaa tacatgactc aaatagatgc tgataatgct gggtatagtc gtgcccctcg
     5401 agggaatcaa tacgctcgtc cctaattagt tgcattgtaa tagtttaaga tcagccttcg
     5461 ggcttttctt tttaaacgca aaagaacata agttcgtata cttctattga aaatacggat
     5521 tttacatcta ttccctctct atatgtacca aaagaattta actatcgtac taatgacata
     5581 gcaatatgaa aggactgatt ttatgcgtgg cggtgtgaga aaacgtggaa aacgttggta
     5641 ttattattt
//
LOCUS       DACEOM010000125         5579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEOM010000125 DACEOM010000000
VERSION     DACEOM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.132"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            153..2213
                     /locus_tag="IW781_002836"
     CDS             153..2213
                     /locus_tag="IW781_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183801.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2296..2865
                     /locus_tag="IW781_002837"
     CDS             2296..2865
                     /locus_tag="IW781_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183802.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3040..3846)
                     /locus_tag="IW781_002838"
     CDS             complement(3040..3846)
                     /locus_tag="IW781_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183803.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3984..5498
                     /locus_tag="IW781_002839"
     CDS             3984..5498
                     /locus_tag="IW781_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1183804.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1984 a          967 c         1123 g         1505 t
ORIGIN      
        1 aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag
       61 tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat
      121 gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac
      181 taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta
      241 agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg
      301 ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt
      361 cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat
      421 tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta
      481 acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata
      541 tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt
      601 tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt
      661 cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc
      721 tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa
      781 acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt
      841 ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc
      901 aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag
      961 atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa
     1021 tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag
     1081 actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg
     1141 cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag
     1201 aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg
     1261 atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt
     1321 ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag
     1381 tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt
     1441 atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg
     1501 aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac
     1561 cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca
     1621 aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac
     1681 tccatgctca gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac
     1741 aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc
     1801 caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct
     1861 cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg
     1921 aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac
     1981 agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa
     2041 ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac
     2101 ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag
     2161 gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat
     2221 ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg
     2281 aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga
     2341 ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat
     2401 caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg
     2461 acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa
     2521 agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg
     2581 cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat
     2641 taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc
     2701 ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct
     2761 ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca
     2821 gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata
     2881 agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt
     2941 taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg cagtgacaaa
     3001 agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga tttttttagg
     3061 aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga ggtaaccaaa
     3121 tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag tatccatgat
     3181 tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat ctgcattttg
     3241 actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc cgtctttact
     3301 taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga attgcagtaa
     3361 tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg aaaaacagaa
     3421 ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt tattgagcaa
     3481 ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat gtagaaaagt
     3541 atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg ttgaactaaa
     3601 aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta atatcttttc
     3661 cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg tatttcccca
     3721 gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta ataaaatata
     3781 gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg
     3841 attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt gtgtttacca
     3901 aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac ttaatatagt
     3961 ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta acagccgttg
     4021 cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag acaagttcta
     4081 gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat acagatccaa
     4141 aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca gggatggcag
     4201 cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag atgcctgttg
     4261 ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa
     4321 aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta aaaggtggtg
     4381 gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct gatgcggtcg
     4441 attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga gggatgagtg
     4501 aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta gtggaggggt
     4561 tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat gtgacagaca
     4621 ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa
     4681 aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat
     4741 ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa gaaggatcaa
     4801 ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat atggataaaa
     4861 tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag gttgttcgcg
     4921 gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa
     4981 gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat ttgatttttg
     5041 accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa ggatatgtca
     5101 caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa gaaacactag
     5161 caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct caattcaacc
     5221 gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac
     5281 caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa gtattagata
     5341 aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt ggcttacatg
     5401 gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg cgacaagcgg
     5461 gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag
     5521 gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca ctgtttagt
//
LOCUS       DACEOM010000126         5547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEOM010000126 DACEOM010000000
VERSION     DACEOM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.133"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(620..2770)
                     /locus_tag="IW781_002840"
                     /pseudo
     CDS             complement(620..2770)
                     /locus_tag="IW781_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2916..3491
                     /locus_tag="IW781_002841"
     CDS             2916..3491
                     /locus_tag="IW781_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1183805.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3705..4400
                     /gene="vanR"
                     /locus_tag="IW781_002842"
     CDS             3705..4400
                     /gene="vanR"
                     /locus_tag="IW781_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1183806.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4378..5532
                     /locus_tag="IW781_002843"
     CDS             4378..5532
                     /locus_tag="IW781_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1183807.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1733 a         1076 c         1050 g         1688 t
ORIGIN      
        1 cgtcaacact taaccgcctg ttagaaatac ggttaagttt ttcccactca tttacgatta
       61 tgcctttttt actcaaaaat agctcctcct caacgaaaaa agagattctt ggaaagaatt
      121 tccagagttc acgtaactct aaaaatccac atccaaaaat ctctagcttg aagaaaccta
      181 aaaatattcg tataatgaac ttgagttacg gaactaggtg aagtagtttc cgtaaaggtc
      241 gagagtgcgt caacactgct cgactatttt tttactcttt tttctcattt tctttgatta
      301 tagcactcct ctagaaaaaa taaaaagacc tcagggaatt tcattccccg aaccccttga
      361 gcctcctcct gccgaaatag aatggcgctg ccagcattta tttcgttcag ctatcggcta
      421 cacaatctaa cacgaccgtt ccgctaccag tcgccttaca ggctccattt tcgctcctct
      481 aacgtgtttc accgattgtg ccgataaact aaacgaaata aacgctaaaa ccgctcagaa
      541 cgcaaaatat gccattttag agctattgta gaaaatatag tttgaaatgc aattttcttc
      601 tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt ccagttccat
      661 aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc ataccatctg
      721 acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga tgaaagttta
      781 ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga gatacattgg
      841 ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag ccaatattca
      901 ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct ggttttcgat
      961 tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca tccatgagta
     1021 aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat ttttttgctt
     1081 cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca agagaaaccc
     1141 catctaactt attggaattg gcagctaacc actttaacct ttcattaaag ctgctggttc
     1201 tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc cccttcgatt
     1261 gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt ctgctgccaa
     1321 caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca gccatttcat
     1381 agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc catcgttttg
     1441 aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg ttcattcctc
     1501 ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga aattccaata
     1561 ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc agtaagtcta
     1621 aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa gagacaaagg
     1681 tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc tctcttgctt
     1741 taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc tttttaccgt
     1801 tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta agtatttgcc
     1861 tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat aaatagatgg
     1921 ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc tcgtatcttg
     1981 agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt tgcactgttt
     2041 ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa gtttcgggtg
     2101 aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat tcggatggat
     2161 gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga gatttactaa
     2221 ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt ctttcaagtg
     2281 tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat tcatgcagta
     2341 gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat gtcattcgat
     2401 attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc aaatgatccc
     2461 aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca ccaatctgtt
     2521 tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat ggccaaccgg
     2581 gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc ctccttcgct
     2641 tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt atccattcat
     2701 cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct ctacctctcg
     2761 caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc aattatatat
     2821 caatagagtg tactctattg atacaaatgt agtagactga taaaatcata gttaagagcg
     2881 tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt tatattcgtg
     2941 tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag atcggaatgg
     3001 atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa cttcaaaaag
     3061 tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact cgaatcactc
     3121 gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag gcaagtttaa
     3181 aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc caattcttaa
     3241 ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg agacaacgtg
     3301 aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag aagtatcata
     3361 aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat atgactgtaa
     3421 atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa ttatcagaag
     3481 tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa gaaaaggaaa
     3541 ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa aaaagaatca
     3601 tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg gccctataat
     3661 ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc gataaaatac
     3721 ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta aaaaacgaga
     3781 attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata gacaagtctg
     3841 agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt actatctgtc
     3901 aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa gatacagagg
     3961 tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag ccctttcgcc
     4021 cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag
     4081 taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat gttaacaccc
     4141 atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt tcaatactgc
     4201 gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt catgagatat
     4261 ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc cggcatttgc
     4321 gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta tggggggttg
     4381 gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa actttacatg
     4441 tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc
     4501 cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga cttaaatcac
     4561 ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat ctttatttat
     4621 gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa
     4681 tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga taaacaaatt
     4741 gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa acggactctg
     4801 gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac
     4861 ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag cctgcttgac
     4921 gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac gttggacaaa
     4981 gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta taacctacaa
     5041 acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca gatgaccgat
     5101 gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc ccccgaggat
     5161 ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat tttgaaaaac
     5221 gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct ctccggggat
     5281 gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct agctgccata
     5341 tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg cgcgggactt
     5401 ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta cgcggaaagc
     5461 aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt ggttgataaa
     5521 aggaggtcct aagagatgta tataatt
//
LOCUS       DACEOM010000127         5529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEOM010000127 DACEOM010000000
VERSION     DACEOM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.134"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..427)
                     /locus_tag="IW781_002844"
     CDS             complement(137..427)
                     /locus_tag="IW781_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183808.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IW781_002845"
     CDS             complement(545..805)
                     /locus_tag="IW781_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183809.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IW781_002846"
     CDS             complement(942..1355)
                     /locus_tag="IW781_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1183810.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IW781_002847"
     CDS             complement(1811..2296)
                     /locus_tag="IW781_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183811.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="IW781_002848"
     CDS             complement(2435..2650)
                     /locus_tag="IW781_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1183812.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="IW781_002849"
     CDS             2874..3674
                     /locus_tag="IW781_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1183813.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..>5529
                     /locus_tag="IW781_002850"
     CDS             3693..>5529
                     /locus_tag="IW781_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1183814.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKL"
BASE COUNT         2040 a          865 c          977 g         1647 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaa
//
LOCUS       DACEOM010000128         5265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEOM010000128 DACEOM010000000
VERSION     DACEOM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.135"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..549
                     /locus_tag="IW781_002851"
     CDS             <1..549
                     /locus_tag="IW781_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1183815.1"
                     /translation="MLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDP
                     NGRTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNA
                     MARKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEV
                     ISEENEDVQTGLFDASNPPLNK"
     gene            592..1392
                     /locus_tag="IW781_002852"
     CDS             592..1392
                     /locus_tag="IW781_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1183816.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            1407..2258
                     /locus_tag="IW781_002853"
     CDS             1407..2258
                     /locus_tag="IW781_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1183817.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            2255..2614
                     /locus_tag="IW781_002854"
     CDS             2255..2614
                     /locus_tag="IW781_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1183818.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            2629..2790
                     /locus_tag="IW781_002855"
     CDS             2629..2790
                     /locus_tag="IW781_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1183819.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            2787..3113
                     /locus_tag="IW781_002856"
     CDS             2787..3113
                     /locus_tag="IW781_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183820.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLEKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            3110..3925
                     /locus_tag="IW781_002857"
     CDS             3110..3925
                     /locus_tag="IW781_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1183821.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            4064..4375
                     /locus_tag="IW781_002858"
     CDS             4064..4375
                     /locus_tag="IW781_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183822.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            4372..4602
                     /locus_tag="IW781_002859"
     CDS             4372..4602
                     /locus_tag="IW781_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183823.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            4609..4812
                     /locus_tag="IW781_002860"
     CDS             4609..4812
                     /locus_tag="IW781_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1183824.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            4809..5108
                     /locus_tag="IW781_002861"
     CDS             4809..5108
                     /locus_tag="IW781_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183825.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
BASE COUNT         1928 a          834 c         1239 g         1264 t
ORIGIN      
        1 atgcttggct ataaaggata tatccaatta gcccaacgat caggtaaata caaagcatta
       61 aatgtgatcg aagtttacga aggagaacta aaaagctgga accgactgac agaagagttt
      121 gagtttgatc caaatggtag aacgtctgat gaagtcattg gatatgttgg ctatttcgag
      181 ttactgaatg gattcaaaaa aactgtctat tggaccaaac aagaaatcga agctcatcgg
      241 attgctaaca ataaagatcg cgataagaca aagttaagtg gtgtgtgggc atctgattac
      301 aatgcaatgg cacgaaaaac tgttttgaga aatcttcttt ctaaatgggg aatcttgtcc
      361 atcgaaatgc aagaagccac cacatcggat gagagagtcc aaagggttca agaagacggc
      421 agcattattg ctgaaacaga agttgaagaa gatattcctg aaagaaaaga agcagaggtt
      481 atatctgaag aaaatgaaga tgtacaaact ggattatttg atgcatctaa tccgccgtta
      541 aacaaataat gagggagctt tctcccttgc ttttctagag gagaaatacg aatgaacaca
      601 ggatatgtca aattacatcg aaaagtgatg aattcattcg tttggaccaa tccttatatg
      661 tataaattat ggagcttgtg tttgatgaaa gcgagtcatg agaatcgcaa gatacttttt
      721 aatggaaaag agatacaagt gaacagcgga gaattcgtca cagggcgcga tgctatcaca
      781 tctgagatga acgaaggcgt gaaacgtgaa catcaagtga acagcggttc tgtatggaga
      841 tggctaaaac aatttgaaaa aaacggaatg ttgaacatca aatcaactac gaaatacagc
      901 gttatatcaa taaaaaactg gtctttgtac caaagcagtg aacaacaaat gaacatcaaa
      961 cgcacaacaa gtgaacaaca agtgcacaca aacaagaatg aaaagaatta taagaatgaa
     1021 aagaataata aaacatcatc gtcgcaacca cgcaaaaagc gtgtttacga caccgactca
     1081 gtttactaca ttctcgcgga ggagttattc aaacagattt gtcagaatca ggaaatcaaa
     1141 aagccgaatc tgcaaagttg ggcagataac attcggaaaa tgatcgagat cgacaagcgg
     1201 accgaaaacc aggtacgggg aatgattgaa tggagtcagc acaatgtgtt ttgggcatcg
     1261 aatattttat ctgctaaaaa attgcgagaa aaatacgaca caatggcagc gcaagcaaat
     1321 cgtgattata aaacaaaaca aactaaaacg cttgaatacg agaaatttag cacagatgag
     1381 ttgcctattt gagaggaggc gtaggcatgg aaactgttgg ggaaatcatg gaaaagctga
     1441 tacagaaagt gcttgtccaa cgtggcgaat gtcctgaatg cggacagcct ttgtatggat
     1501 ggcgtacgaa gaaccctgat ggctcagaac gttgtaagcc aacctgtatg caatgtgggt
     1561 ataaagcatt gcgagttcag gaagacttac aaacagaacg aatttacaac gagagcctga
     1621 aagcaagagc aatcaatttt ttcaaaggtg gttctgttgt gcctaatcaa gcgttgtttg
     1681 attgcacatt gcagaattat caaattgtcg atcaagaaac aagacaagcg gttgaagtaa
     1741 ccaaacgctt tgttaattca gtcttgttag gaaatccaag tcaccttgtt ttaactggaa
     1801 aacaaggaac aggtaaaagt cacctagcga tggcagcggc ttgggaagtc ttgaagcgat
     1861 caaactacga taaaaaaatc ttatttatcg ggttacagga aatgctggat caaatcaaat
     1921 tttcgtacaa caatcctgaa ctcagaaaaa cgattgaggg atcgttgatt gcagatatca
     1981 aaacagcgga tttagtcatc attgatgata tcggttcaga actaggaaaa gatgcatcag
     2041 atagtcgagc gtttggcata aacacgctaa attcgttctt ggatgcacga cagaacctag
     2101 caacgattat cacaacgaac ttgcttggtg aagaactgaa aaaagcttac ggtacgagaa
     2161 caatatcaag aatgtttgtc aactctgatg gatttacgat ggtattttct caaacagcag
     2221 acaagcgcat aaaaccagtg aaaggtagta tcgcatgaat aaataccgta ataaaaaaac
     2281 tgttcatcga ggtatcaagt ttgattctat cgcggaagca gagtattacg atctagcctt
     2341 gtggcaagct gaagcgaatg gttggaaagt gaagctccag gaaaggtttg agctgatgcc
     2401 gaagtttgaa ctagagggca agaagtatcg caagatcgag tatattcccg acttcacatt
     2461 ttacaaaagc ggcaagctgg tcaaagtcgt agatgttaag gggatgcaga caaaagactt
     2521 taagatcaag gcaaagttgt tctgtcatca atatcaagtg ccgttgatat tagccaaaaa
     2581 atatcggaat acgttcaagg aagagcgttt ttaacgaggt ggtccatcat gacaacagaa
     2641 gaagtgattc aaatgcgaat tcgaagcctt cggcgtgaga ttgacgatct ggaacgaaca
     2701 aaggcagtga tggtcaatga aacggctaga aaggcaatcg atttgcacat agagaattta
     2761 agaagggaaa tccgtagatt ggaggaatga gcgtggataa ggaagcggct tggcgaaaat
     2821 taatgttgct gattcaagat gagaactggc aagaagatga agcagtggtt gctgaagttc
     2881 agcgtctaga aaagattgct aacggacgta tacgaaaaaa gccagacaaa agaaaacagc
     2941 gcaaagggaa aatcgtcgtt gttttacacg aaggcaaaat tttgatgcaa ggaacagcta
     3001 gtgagctgtc tgcagaaact ggatatacgc gtgggactat tcgaacgtac gcttggcgaa
     3061 atcacaccga tcgaaaaggg cacgaatata agtatttgga ggaagaaaaa tgaacgaaaa
     3121 caaattaatc aaattgggtg tagcaggagc agtaatcgta ggtattggag ttatcggagg
     3181 atttaagttc ttcgaaaaaa tcgataatgg atatgtgggt gtgcgctatt caatgaacgg
     3241 cggtatcaaa gatgaagcac tgacgcaagg tgtgaaattt gtagggattg acaaagtgat
     3301 ccaatatcca attcgcttgc aaactatcca atcaaaaaat atttcagtat ctacaagcga
     3361 cggcaaaaag acaacgattg atatcaaata tgactacaaa gttgattcaa ctaaagcagc
     3421 aaaaatgtac aaggaatttg ggaatatcac ttcggaagat atcgaaagtg gatggttaaa
     3481 atctaagctt caaaaggtcg ctcgtgaagt ttatgcgaaa tatagtctgc ttgatgtcct
     3541 ttcaggagat tcctctaaag ttgaagctga ggtattaacg aactttgcta aatcagttga
     3601 atctaaaggg tttgaagtcg aagacgtaac acttggtgtt ccagatgtcg ataaagaaac
     3661 acaaaaatca atcgatgcga tcattcgagc tggtcaagaa aatgaaaaag cgaagctaga
     3721 cgcagaaact gcaaaaactc aagctgatag tgaagcttac aagaaaacaa aagctgcaga
     3781 agcagaggca gaatctaatc gcaaagtcgc cgaatcagta acagacaatt tgattcgtta
     3841 tgaagaagct caagctcgca aaaagcatgg atgggtaaca gtaaatggag cagatactgt
     3901 agttacggat gaagcaggca aataatatgg gattctttat ggctaaaatt ctcttgttct
     3961 taggtttagt aggagcagca tatctcgtgt atgcgctcct ttcccaaact gatgacaaag
     4021 aagatgacaa caacgatgaa atgaaatttt aggaggagaa ataatggacg aactaatcac
     4081 aaaagtagag cagtgggcta aagacaaggg actggatcaa gcagatccaa aagcacagtt
     4141 tttgaaagta gctgaggaat tcggggaaat agcttcggcg atggcaagaa gtaatgatga
     4201 actatttaaa gatagcgtag gagacgttat cgtcacgctg attatccttt ccatgcaaaa
     4261 agggacaaac gtacaagagt gtttagaaat ggcatacaac gaaatcaaag gacgcacagg
     4321 gaaaatggta gatggtgtat tcgtgaagtc gagtgatttg gaggatgtga aatgaaatac
     4381 gaaataccac taagtgaagc gggcattcaa gcaattatca atggtcggga ggttaacata
     4441 gaacttcctg atggtactga attagtcatc agacaaagtt atttgaaaga tatggcagct
     4501 ccagtattaa ttgatcgttt taacgtgact gattctgtgg tagagaacca cttaaaagaa
     4561 tttcgatcaa gtatagacaa cactttcaga ttagggagtt gattgacaat gaacaccaga
     4621 caccgcagag tagcaaaact aagaaaacag gaactaaatg tactaaagac aaagtttgaa
     4681 aaagaatatg gaatttcagc agaagaaaca tataaagtgg caagtcagtt tgttgctgat
     4741 gcaagtgatg ctattcgtaa gtttgggatt tcgatattaa atgatgatcg taaatgggag
     4801 gaaatgagat gaaactaaaa gacggatttt acgctagtag tcacggcatc ggcggtttaa
     4861 tgctagatat gccgacaaag aaccctaaaa cacgtaagaa accaaaattc aaagttggtg
     4921 acatggttcg ctgtgaagca gaagggttca tctatccgtt tcgtggatat gtagagcatc
     4981 tctataatca ctcagcgatc attcgcattg aaaacacgat ggaatgtgac aagtggttag
     5041 cgaaaagcaa agagaattta gctgtagcga gattggtgga tatggaggtt ataaacaatg
     5101 aagtttaaaa tctttgaaga ggacactcgt tataaattag aaaaagaatt aaacgatttt
     5161 gcgaaaaaca atgagattca gcatatatct ttagcaactt ctaagagagg ttatgcaaat
     5221 tactatgcag ctgttgtgag ctatgtaagt cgagaagtgt aactc
//
LOCUS       DACEOM010000129         5024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEOM010000129 DACEOM010000000
VERSION     DACEOM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.136"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            282..521
                     /locus_tag="IW781_002862"
     CDS             282..521
                     /locus_tag="IW781_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183826.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IW781_002863"
     CDS             complement(883..1143)
                     /locus_tag="IW781_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183827.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IW781_002864"
     CDS             complement(1328..1825)
                     /locus_tag="IW781_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1183828.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IW781_002865"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IW781_002865"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1183829.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IW781_002866"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IW781_002866"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1183830.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..>5024)
                     /locus_tag="IW781_002867"
     CDS             complement(4547..>5024)
                     /locus_tag="IW781_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1183831.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1529 a         1042 c          771 g         1682 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaa
//
LOCUS       DACEOM010000130         4994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEOM010000130 DACEOM010000000
VERSION     DACEOM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.138"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            85..4746
                     /locus_tag="IW781_002868"
     CDS             85..4746
                     /locus_tag="IW781_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAQ1183832.1"
                     /translation="MSYSSYDHDDLELASTMRIERRIYFESGKADLSEMVKLPLAELL
                     SLRAESAAAEQEVFDRLKEQAAAWEEQAGKTLFLDKALEYARTLPVTHTANQWEAPDE
                     YRHIRSNMVYQMDYSISENTRYDSTAKKSVPYSWTLRWGLYTNAPHGNQREKIAGQDR
                     KVFANREELDRYLNGRIKAHDHYFTEISPAIPKEYADCFKVNGCLLPGYTIEGEEPAK
                     TAELPTQEEAAQPQTAATPERREPVNEIFSIFLDNRAEAQTGGPHGYWLSLPTSAEQV
                     QEALKEIHITANNQQDLFIGGFSAPEGKPLELPEDLIKAASVDELNFLAAQLQKLDAV
                     ELAELNAAMQSPAKMQTIGQLLDYAENTDCFVLINAKDNRSLGEYYLNDSGLFVVPDP
                     WKPAIDTDRLGSFIANEEQGTFTDYGYILRTSDEWQRVYEGQPVPEEYRVMAYPAPEL
                     LREESKVQPEAAAPAKTPQPVTPILLNGQNSAERMKEITDRLETGIQELFESERYKAY
                     LTTMSKFHSYSFNNTLLIAMQGGQLVAGYNKWRDDFHRNVKKGEKAIKILAPAPFKAK
                     KEVQKLDAQGRPVMGKDGKPVTEVQEIQVPAFKIVSVFDVSQTEGEPLPSIGVEELTG
                     SVERYGEFFKALEQTSPVPIGFEDIPGGSHGYYHLTEKRIAIQEGMSELQTLKTAIHE
                     IAHSKLHAIDPEAPAIEQADRPDSRTREVQAESVAYAVCQHYGLDTSDYSFGYVAGWS
                     SGKDLKELKASLETIRATAHELITTIDGHLAQLQQQRQTQQEQPQAAPLEQAAEQPDP
                     DSVFSKLSPEQQQEMTDSVKAMLQTLIDADLKSTGEVSQGTKEAAQAQGFTIAGDGTL
                     EQAKAPQEAAYRLESGEYLYIQTSETGYDYTLYGPDYKELDGGQLDNPDLSLAEVGKE
                     ILAIHERPAGAMEPLTGDRLDDFLEATEQANAIPQPQAWNGIDGLLNGKPFMPEASPA
                     DRAAALIELAEKNAPRLGSEERQLIVAYAEAVGDTDKVIGLINRLCEQGYELQKGQMD
                     SFVKSEIESEIAVANAQRQIAQNPAAEPVVTILWSESPHLKDGQQMPLHEAEAVFKEL
                     DSARRHDREQPGYTGHWYDKTKFRIDFTMQGQPDSYEGRQDFGDGDGSLIQHIRGYHK
                     YYAQDESWKNHVLHHEGPEAWEADKAQRDMLLHEFVPYMELHCNLSAMEQEARRPLRS
                     GETLTPEQTAYFNAVLDYVKECRPLLNQGQYHLPEPPQLADFDQSLQDYKKQIEAELE
                     QEAAAAGLTVEEYVASDYEAPAQPNFSIYQVPPGPEGRDFRYRSYEDLQADGLSVDRK
                     NYQLVYTAPLDKDTTLDEIYRRFNIEHPSDYKGHSLSTGDIVVFRQDGKQTAYYVDEG
                     ADYRQVPEFFAQPEKQLTPDECMTGEQIQTPRGRFYLTDRSREQMEAAGYGFHHQSED
                     GRYLIMANGTRAFAIPAQQESHIKTAEMSTEQNYNMIDGMMNNAPSMEELEARAKAGE
                     QISLLDVAEAAKAEAKKPKQTRKTTQKQKKPSIRAQLAAAKEEQKKKPPAREKSKEME
                     V"
     gene            4750..4950
                     /locus_tag="IW781_002869"
     CDS             4750..4950
                     /locus_tag="IW781_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183833.1"
                     /translation="MYFTVEEENLICLYHNADRRKTAANLRAALPDMDKEMAVLACQT
                     ADKLDAMSDADFAAQRFHFTDE"
BASE COUNT         1265 a         1440 c         1445 g          844 t
ORIGIN      
        1 ttattatcgg cccacccagc aggaaatttt tcaaaggttt cctgcttttc ttatgccatt
       61 tttcagaaag gatgtgaacc ccaaatgtct tacagctctt atgaccatga cgatttagaa
      121 ctggccagca ccatgcggat cgagcgccgg atttactttg aaagcggcaa ggccgatctt
      181 tccgaaatgg taaagctgcc ccttgcggag cttctttccc tgcgggcgga aagcgccgcc
      241 gcagaacagg aagtgtttga ccgcctgaaa gagcaggccg ccgcatggga ggaacaggcc
      301 ggaaaaaccc tgtttctgga caaagccctt gaatatgccc ggacgcttcc cgtcacccac
      361 actgcaaacc agtgggaggc cccggacgaa taccgccata tccgcagcaa catggtttac
      421 cagatggatt attccatttc cgagaatacc cggtatgaca gcaccgcaaa aaaatccgtt
      481 ccctactcgt ggacgctccg ctggggcctt tataccaacg cgccccacgg aaaccaacgg
      541 gaaaagatcg ccggacagga tcggaaagtc tttgccaacc gggaagaact ggacagatac
      601 ctgaatggcc ggatcaaagc ccacgaccat tactttaccg aaatttcccc ggccatcccc
      661 aaggaatacg ccgactgttt caaggtcaac ggctgcctgc tccccggcta caccatcgag
      721 ggggaggaac cggcaaagac cgcagagctt cccacacagg aggaagccgc acagccgcag
      781 accgcagcca ccccggaaag gagggaaccc gtaaacgaaa tattttccat ttttcttgat
      841 aaccgcgccg aagcgcagac cggcgggccg catgggtact ggctttccct gccgacttcc
      901 gccgaacagg tgcaggaagc cctaaaggaa atccatatca ccgccaacaa ccagcaggac
      961 ttgttcattg gcggcttttc tgccccggag ggaaaaccgc tggaactgcc ggaggatttg
     1021 ataaaagccg ccagtgtgga tgaactgaac ttccttgccg cccagcttca aaagctggac
     1081 gccgtggagc ttgcggagct gaacgccgcc atgcagtccc cggcaaaaat gcagaccatc
     1141 gggcagcttt tggactacgc cgaaaacacc gattgttttg tcttaatcaa cgccaaggac
     1201 aaccgcagtc tgggggaata ttatctgaat gattccgggc tgtttgtcgt ccccgacccg
     1261 tggaaaccgg ccattgacac cgaccggctg ggaagtttta ttgccaacga ggaacaggga
     1321 acctttaccg attatggcta tatcctgcga accagcgacg aatggcagcg cgtctatgag
     1381 ggacagccgg tgccggagga ataccgggtg atggcctatc ccgcgccgga acttctgcgg
     1441 gaggaaagca aggtgcagcc ggaggcggca gctcccgcaa agaccccgca gcctgtcacc
     1501 cccattctct taaacggcca gaacagcgcc gagcgcatga aagagatcac cgaccggctg
     1561 gaaaccggca tacaggagct tttcgagagt gaacgctaca aggcctatct caccaccatg
     1621 tccaagttcc acagttacag tttcaacaat accctcctga tcgccatgca gggcggacag
     1681 cttgtggcgg gctacaacaa gtggcgggac gattttcacc gcaatgtgaa gaaaggcgaa
     1741 aaggccatca agatactggc cccggccccg ttcaaggcga aaaaggaagt gcaaaagctg
     1801 gatgcacagg gcaggccggt gatgggaaag gacggaaagc ctgttaccga agtacaggaa
     1861 atacaggttc cggcctttaa gatcgtttct gtctttgatg tgagccagac cgagggggaa
     1921 ccgctgccct ctatcggcgt ggaagaactg accggcagcg tggaacgcta cggggaattt
     1981 ttcaaggcgc tggaacagac ctccccggtt cccattggtt ttgaggacat tccgggcggt
     2041 tcccacggct actaccacct gacggaaaag cggatcgcca ttcaggaggg catgagcgaa
     2101 ttacagaccc taaagacggc tatccatgag atcgcccatt ccaagctcca cgccatcgac
     2161 ccggaagccc cggccataga gcaggccgac cgccccgaca gccgcacccg tgaagtgcag
     2221 gccgaaagcg tggcctatgc cgtctgccag cactacgggc tggacacctc cgattattcc
     2281 tttggctatg tggccggttg gagttccggc aaggacttga aagagctgaa agcctccctt
     2341 gaaaccatcc gggccaccgc ccatgaactg attaccacca ttgacggcca ccttgcccag
     2401 cttcagcaac agcggcagac ccagcaggag cagccacagg ccgcgccgtt agaacaggcc
     2461 gccgagcagc cagacccgga cagcgtattt tccaagctgt ccccggagca acagcaggaa
     2521 atgaccgaca gcgtaaaagc catgctgcaa accctgattg acgcggattt gaaatccacc
     2581 ggcgaagtgt cgcagggaac caaggaagcg gcgcaggcgc agggctttac cattgccggg
     2641 gatggaacgc tggaacaggc caaagccccg caggaggccg cctaccgtct ggaaagcggc
     2701 gaatatctgt atatccagac atcggaaacc ggctatgatt acacgctgta cggccccgac
     2761 tacaaagaac tggacggagg ccagcttgac aatcccgacc tgtcccttgc ggaagtcgga
     2821 aaagaaatcc ttgccatcca tgagcgaccg gctggggcga tggaacccct gaccggcgac
     2881 aggctggacg attttctgga agcaaccgaa caggccaacg ccattcccca gccgcaggca
     2941 tggaacggga tcgacggcct tttgaatggg aaaccgttca tgccggaggc gtccccggct
     3001 gaccgggccg ccgccctgat cgagctggcg gagaaaaacg ccccgcgtct gggcagcgag
     3061 gaacggcagt tgatcgtggc ctatgccgag gccgtgggcg atactgacaa ggtgatcggg
     3121 cttatcaacc gcctgtgcga acagggctac gagctgcaaa agggtcagat ggattccttt
     3181 gtgaaaagcg agatcgaaag tgagattgcc gttgccaacg cccaacggca gatcgcgcag
     3241 aatccggcag cggagccggt tgtcacgatc ctttggagcg aaagccccca tctgaaagac
     3301 ggccagcaaa tgccgctgca tgaagccgag gccgttttca aggagctgga cagcgccaga
     3361 cgccatgacc gagagcagcc gggctatacg ggccattggt acgacaagac caagttccgc
     3421 attgatttta ccatgcaggg acagccggac agctacgagg ggcggcagga ttttggcgac
     3481 ggggacggtt ccctgattca gcatatccgg ggctatcaca aatactacgc acaggatgaa
     3541 agctggaaaa accatgtgct gcaccatgaa gggccggagg catgggaggc ggacaaggcc
     3601 cagcgggata tgctactgca tgagtttgtc ccgtatatgg agctgcactg caatctgtcc
     3661 gctatggagc aggaggcccg gcgtccgctg cgatccgggg aaaccctgac gccggaacag
     3721 accgcttatt tcaacgcggt tctggactat gtaaaggagt gccgcccgct tctcaatcag
     3781 ggccagtacc atttgccgga gccgccgcag cttgccgact tcgaccagag cttgcaggac
     3841 tacaaaaaac agatcgaggc agagctggaa caggaggccg ccgctgccgg gctgacggtg
     3901 gaggaatatg ttgcctctga ttatgaagca ccagcgcagc ccaacttttc catctaccaa
     3961 gtgccgcccg gcccggaggg gcgcgacttc cgctatcgtt cctatgagga cttgcaggcg
     4021 gacgggcttt cggtggacag gaagaattac cagcttgtct acaccgcgcc gctggacaag
     4081 gacaccacac tggacgagat ttaccgccgc ttcaacatag aacatccctc cgattacaag
     4141 ggacattccc tctctacggg ggatattgtg gtgttccggc aggatgggaa acagactgcc
     4201 tactatgtgg acgagggggc cgactaccgg caggtgccgg agtttttcgc ccagccggaa
     4261 aagcagctca cgccggacga gtgcatgacc ggcgaacaaa tccagacgcc aaggggccgt
     4321 ttctatctga ccgacaggag ccgggaacag atggaggccg ccgggtatgg attccaccac
     4381 cagtcggagg acggcaggta tcttattatg gcaaacggca caagggcctt tgccatcccc
     4441 gcccagcagg aaagccatat caagacggcg gaaatgtcca ccgagcagaa ctacaacatg
     4501 atcgatggca tgatgaacaa tgccccgtct atggaggaac tggaagcaag ggcaaaagcc
     4561 ggggaacaga tttccctttt ggatgtggcc gaggcagcca aggcggaggc caaaaagccc
     4621 aagcagaccc gaaagacgac gcagaagcaa aagaaaccgt ccatccgggc gcagcttgcc
     4681 gccgccaagg aggaacagaa aaagaaaccc ccggcgcggg aaaaatcaaa agaaatggag
     4741 gtatgaggaa tgtatttcac cgtggaagaa gaaaacctga tctgtctgta ccacaacgcc
     4801 gaccgccgca agacggcggc aaatctccgg gcagctttgc cggatatgga caaggaaatg
     4861 gcggtgctgg cctgccagac cgccgataag ctggacgcca tgagcgacgc cgactttgcg
     4921 gcgcagcggt tccacttcac agacgaataa gcaaaacgcc gggtgcgggg gctgtcaaag
     4981 cctctgcgtc cggc
//
LOCUS       DACEOM010000131         4866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEOM010000131 DACEOM010000000
VERSION     DACEOM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.139"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IW781_002870"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IW781_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1183834.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IW781_002871"
     CDS             complement(3250..3768)
                     /locus_tag="IW781_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1183835.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1605 a          792 c          743 g         1726 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccacttagct tggataggct acaattaact agacagaaaa attaaggtgt gtagactaga
     4861 agaaaa
//
LOCUS       DACEOM010000132         4840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEOM010000132 DACEOM010000000
VERSION     DACEOM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.140"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IW781_002872"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IW781_002872"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1183836.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IW781_002873"
     CDS             1007..1927
                     /locus_tag="IW781_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1183837.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IW781_002874"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IW781_002874"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1183838.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IW781_002875"
     CDS             3233..4711
                     /locus_tag="IW781_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1183839.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1712 a          772 c          926 g         1430 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt
//
LOCUS       DACEOM010000133         4828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEOM010000133 DACEOM010000000
VERSION     DACEOM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.141"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..106
                     /locus_tag="IW781_002876"
     CDS             <1..106
                     /locus_tag="IW781_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1183840.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(329..559)
                     /locus_tag="IW781_002877"
     CDS             complement(329..559)
                     /locus_tag="IW781_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1183841.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(789..1607)
                     /locus_tag="IW781_002878"
     CDS             complement(789..1607)
                     /locus_tag="IW781_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1183842.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1768..2457
                     /locus_tag="IW781_002879"
     CDS             1768..2457
                     /locus_tag="IW781_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1183843.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2471..3973
                     /locus_tag="IW781_002880"
     CDS             2471..3973
                     /locus_tag="IW781_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1183844.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3986..4462
                     /locus_tag="IW781_002881"
     CDS             3986..4462
                     /locus_tag="IW781_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1183845.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4559..4669)
                     /locus_tag="IW781_002882"
                     /pseudo
     CDS             complement(4559..4669)
                     /locus_tag="IW781_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1631 a          690 c          848 g         1659 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagagtata
      121 tttacttcag atggtaaggc aacaaaataa aatacaatag gcagatgaac taataaataa
      181 aatctgtatt aaatacagat aaattaatat aaaaatcaaa aggatcataa acttttaaca
      241 agtgatgaaa tgggtggaaa ttgattctta tagacagttt gtctcataac attgaagtat
      301 tttgcaattt tagcattgaa acactttttt aatcttcaac gttgttctta taaacaagat
      361 gattgggaat cttttgaatt aacactgtat agcttagaac accctttata gaaaacattc
      421 gcttagcaat cttaataatt ccttgtgccc gatgtcgttt agattcagta ggttcctgct
      481 catcgatcat ggaaaaaatt tgaaggatca gatccttaat aaatttattt aagagaggat
      541 gatccattgc ttcatccata ctcgataaac tagtgataat gagctacacg tttttctttc
      601 aagttgatca atagcttcca cgataaaaat atctttagat aaaatcaaga accgtaggaa
      661 ctacagggat atttggtcaa taagagccac cgctattagt aaaaaataag ctatcaagta
      721 tgctctcatc ccaagaattg cctacttcaa tcgttaaaaa ccgttaggtt tagctaagtg
      781 ggggtagttc actcaatact tttatcaata atagagcgca aaactttttc tcttaattca
      841 actgaatcaa tattattgtt ttgttcataa ccatgaacta atagatcaca aatataaagt
      901 tgtgatattt ttccagctaa agatccacca ttaagaaatt cttcaatggc tgtttgtaaa
      961 actaagtctg cagactttcc tataggtgaa tgaatatagt tagttatagc aataattttt
     1021 gcgccatttt ttttagctat tttcaatgaa tcataagtat ctttcgtttt cccagataaa
     1081 gaaaaaatga ttactaaatc attaactgtt aataaagaag cgacttgggc ttgaaaatga
     1141 gggtctagta cagcttttgc ttgaactcct acgcgaagaa acatattttc taaatcaaga
     1201 ctagtatttc cactagatcc cactccaaat atatatatgt tttttgattg agtgatcatt
     1261 ttaatggctt cattcaattt ttcttcgtct aaaagcctaa tagtggaata gagagcttct
     1321 gttaaactat tagcaatttc atcatagtac tttacactag tcggatgttc ttttttttga
     1381 ctaaagtctt cctttgcaat ttcaattttt aaatcagaaa aaccagaaaa accaattttt
     1441 tgacaaaagc gaataatagt agcatcgcca acttttgtgt ttttcttaat atcactcatc
     1501 gtactataaa taatagtttt cccagaattt aaaataaaat cagcgacttt tttttcagac
     1561 ttcgttaaaa atggataatt ttctttaatt aaatagatat aactcataca aactcctcta
     1621 caatttcaac ttttctccat tatactccaa aataataaaa aaaactccta aatactccat
     1681 ttttatcttt acttttatga aagcggatgc tatattgttt ttggagttgt tatccaaaaa
     1741 taataaaaaa ttggagtgtt taggataatg gataaaaaga ggtttttaga tcttatttca
     1801 aaaggtttga ttgtttcctg tcaagcatta cctggtgaac cattttacaa agaggaagaa
     1861 agtttaatgc cttattttgc tttggctgct caaagggctg gagcagttgg tatacgtgca
     1921 aattctgttc gagatattat tgaaattaga aagtgtgtat cattaccaat gattggaatt
     1981 attaaaagag attatccacc agaatctcca ttcattactg ccactatgag agaagtagat
     2041 gaattagtag aaaccggtgt tgaggtaatc gctcttgatt gcactcttag aaatcgacat
     2101 gatggactat ctataaattc ttttattact aaaataaaag aaaaatatcc tcatcagtta
     2161 tttatggcag atatttctac atttgaagaa ggcaaaaatg catataaagc aggaatagat
     2221 tttgtaggaa ctactttaag tggatatact gaggaaagta aaaaacaaga tgggccagat
     2281 atagatttga ttaaaaaatt ggtagaagaa gggatttgtg taattgctga gggaaggatt
     2341 catactccag aacaagcaaa agagataaaa aaaataggag ttgcaggtat tgttgtgggt
     2401 ggtgctatta ctcgaccaca ggaaattgca tcaagatttg ttaaagcatt aagttagggg
     2461 gagaaataaa atgtttaaaa aattttcaca attaggacgc gctttcatgt taccaattgc
     2521 tatattacca gtagccggat tactattggg actgggagga gcgttaacaa atgagagtgc
     2581 tataaatgct tatccatttt tagatcaacc gtggttgcat acaattttaa gtattatgag
     2641 ttatgctgga aatgcagttt ttgcaaacct agcactaatt tttgcaattg gtattgctgt
     2701 aggacttgca aatggagata aaggaacagc tggtttagct ggtggagtag cttatctagt
     2761 atatactgca actattagtg ggtttcttga acttttttca gcaaaagatg caacactaga
     2821 tacaggtgta gtgggatcaa ttgttgttgg tggtactgtt gcatttttac acaatcgtta
     2881 tagaaaaatt gaattaccac aattcttagg tttttttgga ggatcaagat ttattccgat
     2941 tgtttctagt ttagctgcaa tcattattgg ttctttcttt tatttaattt ggccaccaat
     3001 tcaaaatggt ttagtagttg ctggtgaaca aattgcccaa atgggtagtt taggaacttt
     3061 tttatatggt tttttattgc gtttaacagg tgctgttgga ctacaccaca ccatttaccc
     3121 tttgttctgg tatacatctt taggtggtac cgaaactgta gctggatcaa caattgctgg
     3181 agcccaaaat attttctttg cccaactagc tgatccaaac cacactggtc tgtttactta
     3241 tggtactaga ttttttgcag gaagatttgc aacaatgatg tttggattac cagcagcatg
     3301 ttatgcaatg tatcgtgcaa ttcctaagaa aaatcgtaaa aagaatggtg ggctttattt
     3361 tagcggggcc cttacatcat ttttaactgg aattactgaa ccagttgaat atatgttttt
     3421 atttgttgct ccttggcttt atgtgattca tgcattctta gatggattat ctttttattt
     3481 tgctgatatc ttaaatattc gtgtagggaa ttccttttca ggtggtttga tcgacttttt
     3541 attattcggt gtacttcaag ggaatgacaa aactaattgg ttaaaattaa ttccttttgg
     3601 tattgcatgg gcatttatat atttctttgt ttttacattc tttatcaaaa aatttaaggt
     3661 agctattcct ggaatggaag aagaatcaga agttcattct gaaaaagtaa taaacaaaaa
     3721 tacatcatta catgaagagt ctcttatcat tattgaagct cttggtggaa aagaaaatat
     3781 tgagtctgtt gaagcttgtg caactcgttt gcgtgttgct gttacgaatg gggagattat
     3841 tcaaaaacaa acaataaaag acactggtgc aactgctgtc ttcgaagtga agggtggcat
     3901 ccaagctgtt tttggaggga aagctgactt attaagtcaa gaaatcaatc aaatattagg
     3961 aaaagataat taggaggttt ttaatatgtt tgaaattttc aaaaagaaaa aacaagaaac
     4021 tttttatgca ccttgttccg gaaaaatcat ttccatcaca gaagtaaatg atcctgtctt
     4081 ttcacaaaaa ttactaggtg atggttttgc cgtcatacct gatggttcaa aaatttatgc
     4141 tccattggct ggtcgagtaa caaatatatt tcctactaaa catgctatta atataaaaag
     4201 caactcaggc attgattatt tgattcatat tggaattgat acagttgaat tacaaggaga
     4261 gcctttttcg atttgtgttc aagaaaatga tgaggttact cctgaaacct cgttggttga
     4321 tgtggatttt gacaaaataa taaaatctgg taaagatact tctgttatag ttgtttttcc
     4381 ggagaaaaat cagttcaatg atttaataat agataatggt cgaattgctc atgggaattc
     4441 atgtggaaaa atcacttttt aaaaaagtgt atataaaaag ctataaaata agctaaatag
     4501 gatatttgtc aaatgaagtt ggtgaacaga aagaactgtt ttctttctgt tcattttttt
     4561 attttattag taatgattcc ccatgccaaa tcatgatccg taatttaaag ttataaaagc
     4621 ttcgaaatcc attggcattt cgttggaata ccttgatatg attattcaaa tataaaatat
     4681 agagagaaga gagagaggga agatatttat aaagggcaaa gttatgttta ctcaacttag
     4741 gaaatagaaa tagatcaatt tatattctct tgtcaatcga atatgaacct tagcactata
     4801 taccaaatat agtaaaatgc tgaaagat
//
LOCUS       DACEOM010000134         4817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEOM010000134 DACEOM010000000
VERSION     DACEOM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.142"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(207..1169)
                     /locus_tag="IW781_002883"
     CDS             complement(207..1169)
                     /locus_tag="IW781_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1183846.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IW781_002884"
     CDS             complement(1171..2610)
                     /locus_tag="IW781_002884"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1183847.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IW781_002885"
     CDS             2795..4741
                     /locus_tag="IW781_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1183848.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1538 a          822 c          793 g         1664 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagctggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataa
//
LOCUS       DACEOM010000135         4806 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEOM010000135 DACEOM010000000
VERSION     DACEOM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.143"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IW781_002886"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IW781_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1183849.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="IW781_002887"
     CDS             complement(2064..3062)
                     /locus_tag="IW781_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1183850.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="IW781_002888"
     CDS             complement(3288..3941)
                     /locus_tag="IW781_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1183851.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IW781_002889"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IW781_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1183852.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1414 a         1007 c          745 g         1640 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaa
//
LOCUS       DACEOM010000136         4763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEOM010000136 DACEOM010000000
VERSION     DACEOM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.144"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(127..522)
                     /locus_tag="IW781_002890"
     CDS             complement(127..522)
                     /locus_tag="IW781_002890"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ1183853.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(532..1380)
                     /locus_tag="IW781_002891"
     CDS             complement(532..1380)
                     /locus_tag="IW781_002891"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ1183854.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1395..2222)
                     /gene="panB"
                     /locus_tag="IW781_002892"
     CDS             complement(1395..2222)
                     /gene="panB"
                     /locus_tag="IW781_002892"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ1183855.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2234..3094)
                     /locus_tag="IW781_002893"
     CDS             complement(2234..3094)
                     /locus_tag="IW781_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAQ1183856.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3332..4501
                     /locus_tag="IW781_002894"
     CDS             3332..4501
                     /locus_tag="IW781_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1183857.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1551 a          892 c          583 g         1737 t
ORIGIN      
        1 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
       61 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      121 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      181 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      241 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      301 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      361 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      421 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      481 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      541 ttcaaatgta aaattatcta ttaatcttgt ttttcctata aatactgcta ttgcaactaa
      601 aactgatttt tcaatataat ttactctttg taatgataaa gaatcaacaa cttctacata
      661 atcaatcttt gccaaattat aagtatttat atttttactt attatctcaa ttatttttaa
      721 gctgtctctc tctccattat ttaaagcttc cttagctaaa gttaaagatt tatttaaaat
      781 agtagctgaa gatctttcct ctaaacttaa gtatgtgttt ctagaacttt tagctagtcc
      841 atcctcttcc cttataattg gacaccctac aacgtctata tcaatattaa ggtccctaac
      901 cattctttta attacagcta actgttgtgc atccttttct ccaaaataag ctctatctgc
      961 tggtattatg ttaaataatt tacttacaac agtgcaaacc cctctaaaat gtattggcct
     1021 actagctcca cataaacctt ctgttaatcc agttatatta acaaaagtac ttgcattgtc
     1081 acaatacatc tcttcaactt caggattaaa tattgctgtt gctctacctc ttgtgcatac
     1141 ttctatatct ctttctaagt ctcttggata actacctaaa tcttcatttt tcccaaactg
     1201 agttggattt acaaagatac ttacaacaac tttatcattt tcttttgatg cttttctaat
     1261 taaactttca tgaccttcat gtaaatatcc catagtagga actaatccta cctttaagcc
     1321 ttgttttttc cactctttta cttgacttct aacatcattc actgttttaa atataaccat
     1381 atttcttgac ccttttaata taatttttct aaaacatcat cacttatttt aaacccatgt
     1441 tctggacctg gaaaaactcc atcttttact tcctctatat attttgtaaa tgctttattc
     1501 atctcttctg ataaattagc atatttcttc acaaatttag gggtaaaatc actatacatc
     1561 cccaacatat cttgatatac aagaatttgt ccatcacacc ctgcacctgc tccaatacca
     1621 attgtaggta ttgatatctc tttagaaatt aactctgcaa gcttagttgg aacacattct
     1681 aacactacag cgaatgctcc tgctttttct acagctttag cagctcttat taattcttta
     1741 gcagcttcct tgtcctttcc ttgtacctta aatcctccaa atgcattaac agattgaggt
     1801 gttaaaccta tatgcgccat aacaggtata gaagccttta ctatagcctc tattttatcg
     1861 catacttcta tccctccttc taatttaaca acctgagctc gtccttcttt tattaatctt
     1921 cctgcattaa ctactgaatc atatactgaa gtttgataag acataaatgg catatctgca
     1981 acaactaaag tgttcttagc tcctcttgta actgctctag tatgatgaat catgtcttcc
     2041 attgttactg aaagtgtatc ttcatgtcct agacatacca taccaagaga atctcctact
     2101 aatattccat ttatcccagc ttcatcaatt atttttgctg ttgaatagtc ataagctgta
     2161 agcattgtta atttttcatt tcttaattta gattctttaa atgtaactgc tgtatttttc
     2221 atctatttct ctcctaactc tctcctaaat acttttctaa ttcactataa tctctattaa
     2281 tattttttaa catagctatc tctaatacat tttttgataa tagcatatat aaagctctgt
     2341 cttcatctga taaaacttca cagtgacctt taattgttga aatatcacaa cgttcaactg
     2401 gccctgtcaa actttctata attccttttt cttttatatt tctaatatta aattcaatta
     2461 atggatataa agcttcaatt gccatttttt cagtaaatcc acattgaatt agatatgtaa
     2521 cgcttgtatt aataagtcct agcacaaggt tactagatac tactgatgcg gcatgataaa
     2581 gagatttatt ctccttagat acaattacag tactattacc taactttttg aagaaatcac
     2641 ataaaaactt tgcatatttt tcatcgcctt caattgttat aaaagatttt tttaattttt
     2701 tgtatgaatt atatttatct gatattgaaa acataggatg gacagaatag ccataagcac
     2761 catagtcctg tatatttgaa aagatatctg aagatattgc accactgcaa tggcagatta
     2821 atttgttttt gatttgataa tttttaattt cttgccatac ctcctgaatt tgaccatcag
     2881 gagtcgtaat gaaaatagta tcattttcat ctactaattc tcttaagttt aaatattgtt
     2941 tactagatgt aaaattcgaa gctgctaatg acgagtcttc gcttttacta taaaatcctg
     3001 ttacttgtat tttatgttcc tggaaatact tcccgaatga acaacctacc ttgccggctc
     3061 caataaaacc aacgttcata attatcacct ccataaagaa gtgagactac aagtcccatt
     3121 cttacgatat gagcttaaaa ataaaaatta aatcaaatat acagtatgtt tttttcaaat
     3181 atcatccata gttaatttat cataatatcc catattttaa aataaaagat agcgtaaaat
     3241 attttgtgta aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta
     3301 ccacacagaa attttatagg aggactttct catgactaaa tttactatag aaattatgca
     3361 aacactactc aataaagggg atttagatga attatttcag gatcatttag agcgagcgat
     3421 taattcactt ctacaagcag agctaactgc ttttttagat tacgaaaaat atgatcgaaa
     3481 tggattcaat tcaggaaatt cccgtaacgg gaattattca cgtacattta aaacagaata
     3541 tggcgagttg aatttaacta ttcctagaga tcggaatggc gaattttctc aacaaacact
     3601 accagcatat aagcgaacca atgattcatt agaaacgacc attatccaat tatttcaaaa
     3661 aggcattact atggctgaaa tctccaaatt aattgaaaaa atgtatggtc accactatac
     3721 gccacaaaca atctccaaca tgagtaaatt ggtggctgaa gatgttttag cttttaaaga
     3781 aagaacgtta gaagccaatt attccgttat atttatggat gcgacgcata tccctgtgaa
     3841 gcgacagact gtttcgaagg aagcagtgta tattacgatt ggtattcgtt tggatggaac
     3901 caaagaagtt cttgggttta ctattgcccc aactgagtct gcttatattt ggaaagaagt
     3961 tcttcaagat cttagaaaac gtgggttaga agaagtttta ttagtagtga cagacggatt
     4021 aagctgtatt gaagaaagta tccatagtgt gtatccgaat gcccaatttc aacaatgttg
     4081 tgtgcatgta tctagaaata tcgctcataa agttcgtgtt cgaggtcgaa aagaaatttg
     4141 tgaggatttc aaattggttt accaagcgaa ttcaaaagaa gaggcattgg atcacatcga
     4201 ctttatgact aggaaatgga aaaagcagta tccaagagtc gtcaatttac tcttgaatcc
     4261 tgccctatta accttttata atttccctca cgccatcaga cgaacaattt attcgacgaa
     4321 cctgattgaa ggctttaata agcagctaaa acgatatact cgaagaaaag aacaattccc
     4381 taatgaagaa tctctagaga gattcttcat ttctcaattt aatcaatata accaaaaatt
     4441 tttaggtaga attcacaagg gatttaaaga aattcaggat acattagagt cgatgattta
     4501 actgtaatta acagaatgga ttttccattt acatataatt cttgacgcta ccataatcta
     4561 aataaccatc atattcatct actacttata taattgagtt ttattaaact ttttaagttt
     4621 ctattctatc aaatatttaa acaatctctg catccgaaaa gactacctcg tatagtttta
     4681 ctaacaatat ttttattagc gtgttcaaat cctgatttct tgtatcaatt attttctgtt
     4741 cactttcaat aagaaaaaag tct
//
LOCUS       DACEOM010000137         4480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEOM010000137 DACEOM010000000
VERSION     DACEOM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.145"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(301..831)
                     /locus_tag="IW781_002895"
     CDS             complement(301..831)
                     /locus_tag="IW781_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1183858.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            936..1487
                     /locus_tag="IW781_002896"
     CDS             936..1487
                     /locus_tag="IW781_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1183859.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1640..2113)
                     /locus_tag="IW781_002897"
     CDS             complement(1640..2113)
                     /locus_tag="IW781_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1183860.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2251..3723
                     /locus_tag="IW781_002898"
     CDS             2251..3723
                     /locus_tag="IW781_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1183861.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3720..4430
                     /locus_tag="IW781_002899"
     CDS             3720..4430
                     /locus_tag="IW781_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1183862.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1403 a          817 c          864 g         1396 t
ORIGIN      
        1 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttgat aaacggtgtt
       61 caaacggctg cttctcggta ataagcccac tagcctagaa aatatgaaaa gctattttct
      121 aactagcgtt cttattactc aaagcataac gctgatttca caacctcttg ataaacggtg
      181 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaacatgagg aactattttt
      241 ccgtttcttt ctctaatact caggtcagaa cgctgatttc acaacctctc tttcacaacc
      301 ttattttact cgtttataat tttcatcgaa gtattttccg ctacggatat catctggcat
      361 tccatcgtat ggttcttcgc taataacatc ttcattgtct atacctgcat cacccatata
      421 agtgatcgta gcaaattcag gaacaagtaa ttttggataa gtttcatatt tttcacgagc
      481 tgcttccatt tctgcgatat gttcgttttg gaaagtaaca gtgatttctg gatgttcttc
      541 aacccattta gggaaaaaga tcattccgtg aagtttcttt tcatttggca taaagtccaa
      601 tgccacgtct gtccctgttg gctctgtcca agtgattttg aatacgccgt ccgtcaattt
      661 aacgatatca gcttcttggt cttttaccca acggcctttg accattccgc catggatacg
      721 gtaatcaaca gtatggtcat ttttggcata ccattcatat tcccaaccat tgtcatacgt
      781 atagataaag tgagttccaa taaaatcttc caattcttta aattctttca tgtttaagca
      841 tctccttata aattaaaaca tgtaataaac tacatgtttt cgattactca tttatgatag
      901 aatggattta aagatttgtc aaggagggaa aactgatggc aagaaaaaat acacttcgat
      961 atattttgtt agggttatta agtaagaaga agatgacagg atatgaattg aatcaatcgt
     1021 tcaaaaatga aataggagaa ttttggcaag caaaacatag tcagatttat cctgaacttg
     1081 caaaaatgga agagcaagga atcattcagc atcaagtaga gatcacaggt gaaaaattgg
     1141 aaaagaaagt ttatgaatta acagaagaag ggaaggaaca attggcagag tggattcata
     1201 cacccaccaa tgaactaccg gttaatcgag acgaatttgt tctgaaattg tatttcgtca
     1261 aagacgtgaa tgatccggca ttagtagaaa ttattcagca gcagcgtgac cttcatgaag
     1321 aaaaacgact tcatctatta gagcgaaaaa aaacgatctt tcctactgaa aaagaaaaac
     1381 atgaaaatta cggacactac ctgattttag cgcatgcgat caatcgggaa acagaatatg
     1441 caaattggct aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa aatatccgaa
     1501 cagtgatgga gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg gatattctta
     1561 tgttcaaaca ggcctttttt caagtattta tgtagcatct acttattaaa aagcttggta
     1621 cttaggagat tagtggcgtt tagagttctt ttaacaactg ttcgtagtca gctttttcat
     1681 cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg atgtacatct
     1741 tagcattctc ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg tcgatcaatt
     1801 cgatatcttt gattgctttt gaaaaaactt tacggacatg gtgcattttc ttttggcgga
     1861 tttctgcctt ttctgcagct tttgacaaag taaatccgtc atctaaatat tttttgatca
     1921 gttctacttt tatgactgta ggtaaacgat attttctagg acaattcgca tcagcttcga
     1981 tcgaacgaat gaatcccttt tgttcccaat accttaattg acgtggagac acacctacga
     2041 tatcacttaa ttcactgatg ccaacaacca attgatcatt ttccaacaaa cgtttaaggg
     2101 aagccccaac catttttgtc actccttttg tcctcttgct tcccttaatt atatccgtat
     2161 taacttatgt gtcaagtagg ttgacaaata gcgaatttag gactaaagta ttctagtagt
     2221 ttcagaaaaa attcagaaag aaggagaaat atgagtaaca accaacccgt agatatttac
     2281 ggaaaaccat ataatcggac acttttagtt gttgtcttat taattggtac attttgtacc
     2341 gttttgaatc agaccttatt gacgacagct tttcctacat tgatgaaagc tttcgatatt
     2401 tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg gatcatgatc
     2461 ccaataacag catggctgat caataaattc agttcaagaa atttatatct ttctgccatg
     2521 acgatctttt tgatcggaac gattacttgc tttacagcac ctaattttag tacattacta
     2581 atcggtcgtt tgatccaagc ttgcggtgtg ggagtatcga tgccattatt gcaaaatatc
     2641 atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc agggatcgtt
     2701 attggacttg ctcctgcttt aggccctaca ttatctggtt atatcattga taactaccat
     2761 tggcgtgact tgtttggtat ggtactgcca atcgttgttc ttgtcttggt tttagcattc
     2821 ttcctaatga aaagtgttat tccattgtca aatcctagca ttgatatttt atccgctatt
     2881 ttatcaacga ttggattcgg tagtttactt tacggatttt ctagtgtagg agatgacggc
     2941 tggggaagct tgaaagtaat tggtttctta gttgttggtg tgattttcat cgccttgttc
     3001 tcatggcgtc aattacattt ggaacatcca ttcttggaac ttcgtgtatt caaatcatct
     3061 acattcacga ttgcagctgt tttagctggt gtaacaaata tggcgatggt aggggcagaa
     3121 atggtcgtac cattgtatat tcaaaatatt cgtggagaat cagcattcca ttctggcttg
     3181 atgttgttgc cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg tgcgattttc
     3241 gataaatacg gcgcgaaacg tttagccatc acagggatgt tcattttgac agcggcaact
     3301 gcaccatttg ctttcttgac aaaaacgact ccagttttat atatcgtgat cctttatgcg
     3361 atccgtatgt ttgggatctc catggtcatg atgccggtaa cgacttctgg tatgaatgca
     3421 ttgccaaata acttgatcag tcatgggaca gcggtaaata atacattccg tcaaatcgca
     3481 agctcgattg gtacagctat tttgatcagt gtgttgacga atgtgacaaa agcaaatatc
     3541 ccaagcaaat ctgtattgga taatacgcca ttagcttata aagatcaggc aatcaatgcc
     3601 acactgtcag gttaccatgc cgcgtttatc gtagcgatca tctttgggat tcttggcttt
     3661 attattgcct ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg ggataatgca
     3721 tgattattgg agttttgatt gtcagcttgc ttgcttttgc tttaacgaac gtgttcgcta
     3781 aaaaaacatg gcaaaccttt ttatctttga ttttcggact aatcttcgtc gcttcactaa
     3841 gtttgatcgt cgctaatttg tccaatcact ttggaatgga aaaagtaacg gaaacaaaaa
     3901 cagaaaaaat tgtttccagt gctgattccc aaggagcaga tatgcttttg tataaagctt
     3961 tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa gaaaaaccaa
     4021 aagcaactgg tacggataac gaaacaaata aagtagaaaa gacagacggc gatgctgaaa
     4081 aagtgacgaa aactacttat tgggaataca aaaacgacat gtataaattc tggttcaata
     4141 ttgctgataa caatcacgaa tatgacagcc gagtgaatac attcaaaatc ccagagacat
     4201 gggtagaatt aagtacggac caagcagcca agttagctga attagtgaag aaacagcaaa
     4261 gtacgatgga aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa gcaatgactg
     4321 aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac gaaagcctta gctgctgaat
     4381 tccaacaaca agcattcgca aaattagtga aagaagcaaa aggcgaataa tattttgaac
     4441 accatgtagg aggttgtgaa aaagctgctc tgcatcaagc
//
LOCUS       DACEOM010000138         4370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEOM010000138 DACEOM010000000
VERSION     DACEOM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.146"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..243
                     /locus_tag="IW781_002900"
     CDS             <1..243
                     /locus_tag="IW781_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HAQ1183863.1"
                     /translation="YQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGY
                     KRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            255..524
                     /locus_tag="IW781_002901"
     CDS             255..524
                     /locus_tag="IW781_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183864.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            686..988
                     /locus_tag="IW781_002902"
     CDS             686..988
                     /locus_tag="IW781_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183865.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            985..1146
                     /locus_tag="IW781_002903"
     CDS             985..1146
                     /locus_tag="IW781_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1183866.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1143..1448
                     /locus_tag="IW781_002904"
     CDS             1143..1448
                     /locus_tag="IW781_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183867.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            1448..1804
                     /locus_tag="IW781_002905"
     CDS             1448..1804
                     /locus_tag="IW781_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1183868.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            1764..2009
                     /locus_tag="IW781_002906"
     CDS             1764..2009
                     /locus_tag="IW781_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183869.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2006..2425
                     /locus_tag="IW781_002907"
     CDS             2006..2425
                     /locus_tag="IW781_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ1183870.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            2422..2979
                     /locus_tag="IW781_002908"
     CDS             2422..2979
                     /locus_tag="IW781_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1183871.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            2976..3272
                     /locus_tag="IW781_002909"
     CDS             2976..3272
                     /locus_tag="IW781_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183872.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3349..3765
                     /locus_tag="IW781_002910"
     CDS             3349..3765
                     /locus_tag="IW781_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1183873.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         1598 a          657 c         1014 g         1101 t
ORIGIN      
        1 taccaagagc gaatcgaacg actgtcagag tattgtgaat catctggtaa gacttataaa
       61 aactatttgg caactattcg aagttgggca aggaaagaaa aaagtgaacc taagaacgca
      121 agcagtggat acaagcgcac aggaagacga gagaagcttc ctgaatgggc aatcgaccaa
      181 gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa agcaccattc
      241 taagaggtgg aaaaatgaag atcgattatc tagaactaat taatgaaata gcgaattata
      301 aaaagggcga ggaattagac gtcctgagag acgtatatga tcaactcgaa gaagctggaa
      361 ttgaaggaat taagaatgat cgttcgagtt ggagtaagct cagatactat ttcgcacttt
      421 atatcgatac aacacaatta agaaatttag catataccaa attactattt gttgattgtg
      481 ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaatcagat ggacctaaag
      541 acatttacag cacagattga actaatgcat caagaagctt taagacaaag tgtatcgtac
      601 gaagacaagt ggctcaacac gttccatggc ggacgtgaga gcgcacttga tcaagtactc
      661 aaattactga aaggagaatg tcgggatgga taagaaagcg gcaatgcagc gaattatcga
      721 attgacttat tcagaagatt ggcaaaatga caaagaagct gcttcagaag tgatgaggct
      781 tggaagagcg atgtgggcag acaagagcaa caagccaaga ccacgaaaaa tcgcaatttg
      841 gcacggtgac aaacttctag tgatagggac agctgaacag ttagcaagtc tcacaggctt
      901 gcacgagaaa atcgtgagga aaagagcaag gtgtggctac acagacgcta agaagagaac
      961 gtttagatac gtggaggaat catcatgaca acagaagaag tgattcaaat gcgtattcga
     1021 agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg
     1081 gcgagaaagg caatcgattt gcacatagag aatttaagaa gggaaatcca tcgattggag
     1141 gaatgagcgt ggataagaaa gcagcaatga aaagaattgc tgaattaacc aagtcagaat
     1201 cttggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga
     1261 ctgaaaagcc caaacggaga acgccgagaa aaattgcaat ctggcatgat gaccgaattt
     1321 tagtaacagg tactgctgaa cagttatctg aaattactgg attaagcaaa aacattatct
     1381 gggatagagc taggagctta tggattgatt caaaaggacg acagtttagg tatgtggagg
     1441 agagataatg gatctcatta cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa
     1501 gattcagaaa gataaaaaat caaaagaacg agctgaatta gttaagttag aaatggctga
     1561 aacaggagca ggagtgcgaa gtagcaggca ttggaaagca gcagcaaaca ttgaatttta
     1621 ttacaacgaa attcaaaaag ggttcgatca gatgcgtgag ctggatcggc aaacaaattg
     1681 gagcaagaaa cttcatcaag atcgtttcaa atttgtagag aagtataaag aaatattaga
     1741 agagtatttg aggaggacag caaatgataa aaaaactcgt tcaattcagc atggatttat
     1801 atgatatcga atcaggagca acactatctg tggaatcgga ccatctaatc ataaattttg
     1861 gtggaaagcg ccagattatt ttgtgggtag ttgatgatgt actgtttcca gaaattgttc
     1921 atgatttcga agaatcaaaa gcggttgagt ttgaaatagt gaaaaaagta atggaattga
     1981 ttgaaaaata cgaggaggac agcgaatgat accgaagttt agagcgtggg ataaacgaaa
     2041 gaacgtaatg agagatgtag ccgtcttgca ttttactaaa aacggcaaaa caaactttat
     2101 tgaatattgg ataaatccta ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat
     2161 gcaatccaca ggaatgaaag ataagaatgg tgtggatata tttgaagggg atatagtatt
     2221 agtcagcgtg cgaaatggct tcgattactt agataataaa gtctgtattg tcaaaaattc
     2281 aatagattat tccggattag tttgtgccac tgttgatgaa gacttagagt atcaaatttt
     2341 taacacagag ctgtttgaag aatacacgta cgaagtcacc ggaaatatat acgagaatag
     2401 cgagttattg gaggaacagc gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt
     2461 aaagcgatag gaaatgatgc aatagctgct tgctataacg agagtataaa ttcaggtatt
     2521 acgttaatga aaaaaataga cgaaccgcag aaaccagtta ttccacaact cgtggccggt
     2581 tggcttgaga aatctacgga cccttttaca aaagctgaaa aaatagcgta tttaatcaaa
     2641 tctaaagatg gtgattcata ttatttctgt gattggtttg tacgagatgg catagtgacg
     2701 caagagcaag gagaagaatt actcgcttgg gcaacaagac aatcatatga aacactattg
     2761 agcctataca acggctacga ggttgagaaa gagccgttat gggcaataaa gaatgccgat
     2821 ggaaactatc ttactaaatg tgctttatgg ggaaaagatg gagtaaatta tagttttgaa
     2881 tgcaatccat ctcatcgatt gcttttcact gataaagcaa cagcggatgc tgcagcattg
     2941 ttggtgaatg gaacagtgga agaggtggta gaaagatgaa actaaaagac ggattttacg
     3001 ctagcagtca cggtatcggc ggtttaatgc tagatatgcc gacaaagaac cctaaaacac
     3061 gcaagaaatc aaaattcaaa gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt
     3121 atccgttcag aggatacgtt aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa
     3181 atacaatgaa gtgtgatcgt tcgactgcaa aacacaagca ttatttagct gtagcgagat
     3241 tagttgacat ggaagtaatc aagagcaaat aaaaaaagcc ggatcgctcc gactgattca
     3301 ataaatccaa cacatttatt atatcacata aaggagcggt ttgacttgat gcaattgtta
     3361 cgagaggtag atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg
     3421 cgtttggagc ggatggcagg tcgctctttg atagatatta agtcgccgat tataacggat
     3481 atgccgaagg cgccgaagca cggcaataag gcagaagacg cgatcattca gatgatggat
     3541 atagaagcag agagagatgc gattctagcg gctttgatgg ctcttagtct gattagtcgt
     3601 cagatactct actacagctt ctgtgacgta aacaagcact ctaattatga aatagggcaa
     3661 ttgatacgag gatacggcga aaagaatgta gagaagctga aatctatcgc gctgatcgaa
     3721 tttgcagaag catacaaaaa aggcgtgtta gttcagtatc gttaattttg tagggttttt
     3781 gtagggatag tgtagggttt ttgagcggtt taacgtgata ttatggtagt gtcgaaagat
     3841 taggaaacag gacttcgaca aaataaaacg caagggagga aatctccctc atcgttttaa
     3901 ttaagcttcg atagacagca gcggaaatat taagaataag gatgtgaatt tcaactcctt
     3961 ctaaattgtt cttattatct atcatccgtt gttgtctatt aatttatgta ttggagggta
     4021 agaatatgaa attatcgatt gaaggtactt caggagaaat aaaagagttg ctccaagcta
     4081 ttggtggtag caaggagcaa aatataaaaa ttgatgatat ttataaaaaa agtaaagatt
     4141 ttttaattaa cggtcaagct gttgctccta gtttttaaaa tcttcagaaa cagcggaaag
     4201 aaattgatta taaagcttta tatattcaaa atatgcatca aattttgcat ctttcccttc
     4261 aattatttga gatttaacaa tatctctgac aaaaggtaat gttgcaattg ctaaatcatg
     4321 agcgcgttgt tcattggtta acatagtttt caccttcgta tatttatttc
//
LOCUS       DACEOM010000139         4307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEOM010000139 DACEOM010000000
VERSION     DACEOM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.147"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(48..1853)
                     /gene="glmS"
                     /locus_tag="IW781_002911"
     CDS             complement(48..1853)
                     /gene="glmS"
                     /locus_tag="IW781_002911"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1183874.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2262..2552
                     /locus_tag="IW781_002912"
     CDS             2262..2552
                     /locus_tag="IW781_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183875.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2565..3590
                     /gene="sppA"
                     /locus_tag="IW781_002913"
     CDS             2565..3590
                     /gene="sppA"
                     /locus_tag="IW781_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1183876.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3593..4252
                     /locus_tag="IW781_002914"
     CDS             3593..4252
                     /locus_tag="IW781_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1183877.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
BASE COUNT         1320 a          796 c          906 g         1285 t
ORIGIN      
        1 gaacaaggac cagtgtcaga tgtctgacac tggtccttgt tcttttatta ttctactgtc
       61 actgatttcg ctaaattacg tggtttgtca acgtcgaatc cccgtaatag cgttgcgtaa
      121 tatgcaagta attgagtcgg tacgactgtg gttagagtag ttagataagg gtggacagtt
      181 ggtagcacca gctggtcact ttccttttct aatccctctg agacaataac tagtgtttta
      241 gcgccacggc tttcgacttc tttcaggttt cctcgagtat gcgcaccagt cacttcttca
      301 ctgatgattc cgatcactgg tgttccttct tcgatcaaag caatcgttcc atgtttcaat
      361 tcgccagcgg caaaaccttc tgcctggata taactgattt ctttcaattt caaagcagct
      421 tcaagagaaa cgttgtagtc agcaccacgt ccaatgtaaa aggcatttct tgtatcgctg
      481 agatatactt ctgccaactc ttctaaataa tctttttggg cgatgaccga ttccatcgca
      541 ttagcaacta aactcaattc atgagtgata tcgaatacaa gagaagtttc aaattctttt
      601 ttatcaccga tagctttggc taataaagtt agtacagcta tttgtgcagt ataagctttc
      661 gttgatgcta ctgcgatttc gggtccagca tgcaatagca gtgtaaagga tgcttcccgc
      721 gaaagagtag aaccagcaac atttgtaatc gtaagtgaag ggtagccgag acggttgatt
      781 tttaccagca cttggcggct gtctgctgtt tcgccacttt gactcaagaa aataaagaat
      841 ggtttttttg aaagaagagg tgtgttatag ccaaattcac tggctaaatg aacctcaaca
      901 ggaatctgtg tcaatttttc caaagtctgt ttacctgcta accccgcatg gtaactagtt
      961 ccgcaagcaa cgatatagat tcgatcgcta tcagacaatg tatcgaccag cttttgatca
     1021 agagagacac gattgtcttc tgttacgtat ttttgagcga tacgacgcat gataataggc
     1081 tgttcgtcga tttctttcag catataaaaa ggataggtac ctttctcgat atctgaagca
     1141 tctacttgtg cttcgaatga agaacgttct acgatgtcac ctgcaaaatt ttgaataata
     1201 actttttctg atgtgacggt aaccatctct tcatcttcaa tctcaacgaa ttccttcgta
     1261 tgagcaagca tagccattgc atcgctggca atcacattga atccatcgcc gagtccgatt
     1321 aatagtggac ttttattttt agcgacgtaa ataacatcag gtgctgtccg atcaatcaaa
     1381 gcgaaagcgt aagaaccttt gatgacccgc aaagctttta aaaacgcctc ttgcgttgta
     1441 gtatccttcg cgaaagtttc aattagatga gcgataatct cagtatcggt ctcaccaata
     1501 aagtggtcat ttgctaaata agcaatttta agttcttcaa agttttcgat tacgccgttg
     1561 tgaacgagca cgaagcgtcc actttgggaa gtatgtggat gggcgttttc ttcagaaggt
     1621 tgcccgtgag ttgcccatcg tgtatgacca atacctatat ttcctgtagt atcaatattt
     1681 attttgtttt tcaaattttg aattctgcct tgtgctttga ccagataatc agttgtgcca
     1741 tctgagacaa agataccagc tgaatcataa ccacggtatt ctaatttttc tagaccgtca
     1801 atcaatcctg gtgttacatt ttttaatcca atcattccaa caattccgca cataaaaaac
     1861 atcctacttt ctataatgaa ttcgtatgaa tgcttccagc tgcaggaaaa tccttttgtt
     1921 ttatctttgc agacattttt tgtggatttt ctgtagagtg cagtccctag ggccatccgc
     1981 cgaatctttc gataaaccct ctcctcgtca tctgtttaca cagacctggc gctatcctaa
     2041 aaggtataac tatcctcctt tttgtttttt atctggaaac atttaaaatc ataacctttt
     2101 gcgaatgttc ttgtcaacaa aaaaagaata aaaaataaat tggtatatat caaattcttt
     2161 gtcaaatgtc ctttttatgt caaaaaataa tgggttttta tctatcatct ttttcaggag
     2221 ttcggtacaa tagaaagtaa tcggatagag gaggagtaaa aatggattca aaaattgaga
     2281 tcatgacact gggaatgtta aaaaaacaat tgtccaaatt cgaagcatca gctggtgtat
     2341 cagatgatac gaaaatattt ttagatactg gctgggatag catccaagaa atcgcacctg
     2401 atgccttgga agttgctcaa gctcgtgagt ttacggtaga agatgaatta acaaaagagt
     2461 cgttttccgg gtatgcgcgg gaagaaaaag cagagcggtt cgacacttct gagcagtcag
     2521 aaactgtgat cgtcataaaa aacttgtatt agaaaggaga aagaatgaat aaaagacgtt
     2581 gggcagcagt tctgattgct gcaggattat ttgtggtttc gttagtttca gctagtttga
     2641 ctaagcctca ggaaaacgat caaattgagg gattgaacag ttggttatat ggggatgaag
     2701 aactagctcc aaatgtgttg gaagagggag acagtacgca aaaaatcgta aaattatcag
     2761 tagatggaac gattgctgcg ggagggtctt caggtttatt tgcttctgaa ggttacaatc
     2821 ataaaaattt tatggaacaa ctgaaggcga ttgaagaaga tccaacagtc aaaggtatct
     2881 ttttagaagt aaattctcct ggtggcggtg tctacgaaag tgcggaaatc gctaagaaat
     2941 tggatacgat tcgtaaagaa catgatatcc caatgtatgt cagcatgaaa aacatggcag
     3001 ctagcggtgg ctactatatt tctgctcaag cagataaaat ctttgcaaca gaagaaacag
     3061 tgaccggctc aattggtgtg atcatgtcag gattgaatta ttcaggactt ctagaaaaac
     3121 tgggtgtgga agatacaaca gttaaaagcg gagcgttgaa agatatgggt tcaggaacgc
     3181 gaccagagac aaaagaagaa gaagcggtgc ttcaagcata tatcgacagc gcatatagtc
     3241 gttttgtagc aatcgtcagc gagggacgga ataaatctga agaagaagtc aaaaaaattg
     3301 ctgatggaag gatttatgat ggtgtccaag caaaagaagt aggtttagtc gatgaattgg
     3361 gatttcctga agatgcttta aaagctatac gaaaggaaca acagcttgaa gatgcggaac
     3421 tagtagaata ttcttccacg ccatctgggt ttgctagtac atggttagga agtaaactag
     3481 cagaattcca gggattgaag ccttctgaga ctagtcagat cttatccgtt cttgagaatc
     3541 tagggacagc ggaatcacca aaagcaatgt attattacgg aggtgagtaa gcatgtcaga
     3601 gctttcaaat gaaaataagg aacaagaaaa agataagcaa cagcagcctg ctgtaggacc
     3661 tgactccaac ttttcccaaa aggtactgag ctcttttgaa agagagcctt tgacgcccga
     3721 agaaatcaaa aagaaacaac gtaaatggaa aaagtatacg gacacaagaa atgaaccgcc
     3781 aaaagcaaat gattttccta agtatttcta tgcaggtttt tttatccgaa tgtttgcctt
     3841 cttggtcgat cttttgtgta tcggagctat tacgcgttta acacttggta ttgccaaaaa
     3901 tctaggttgg cttaattttt cagattctta tctgagtctc tacggattca cagccttact
     3961 tatttatcta gcgtatttta ttttattgac gaaattcaat catggccaaa cgattgggaa
     4021 aatgatcttt ggtattcgag tcatcagctt tgatgaagaa gaacttagct ggtcaaccgt
     4081 tttagttaga gaaggtgctt gccggttcat tctaaaattt cctttgctga tgttagggta
     4141 tcttccagca gtctttaatc agaaaaagca acatctgggc gattttttca ctaatacaag
     4201 tgttgttacg atcaatctga tcaaagcgtt caaccaggaa gtcaacagct gatgagcaat
     4261 aaaaagcagt aacagttgaa agcaccttct aaaagtcaga tctaaaa
//
LOCUS       DACEOM010000140         4037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEOM010000140 DACEOM010000000
VERSION     DACEOM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.148"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            854..1066
                     /locus_tag="IW781_002915"
     CDS             854..1066
                     /locus_tag="IW781_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183878.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="IW781_002916"
     CDS             complement(1344..3143)
                     /locus_tag="IW781_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1183879.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="IW781_002917"
     CDS             complement(3267..3863)
                     /locus_tag="IW781_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1183880.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1188 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt
     3961 gtaagttcat cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt
     4021 taataacttg ttttttt
//
LOCUS       DACEOM010000141         3925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEOM010000141 DACEOM010000000
VERSION     DACEOM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.149"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(573..1130)
                     /locus_tag="IW781_002918"
     CDS             complement(573..1130)
                     /locus_tag="IW781_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183881.1"
                     /translation="MRNLSNRNKILIIIVVIAVFPLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IW781_002919"
     CDS             complement(1309..1920)
                     /locus_tag="IW781_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183882.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IW781_002920"
     CDS             complement(2033..2290)
                     /locus_tag="IW781_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183883.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IW781_002921"
     CDS             complement(2887..3165)
                     /locus_tag="IW781_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183884.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IW781_002922"
     CDS             complement(3199..3450)
                     /locus_tag="IW781_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183885.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          521 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagagg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DACEOM010000142         3898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEOM010000142 DACEOM010000000
VERSION     DACEOM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.150"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            406..1095
                     /locus_tag="IW781_002923"
     CDS             406..1095
                     /locus_tag="IW781_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1183886.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1082..2290
                     /locus_tag="IW781_002924"
     CDS             1082..2290
                     /locus_tag="IW781_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1183887.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2584..3855
                     /gene="serS"
                     /locus_tag="IW781_002925"
     CDS             2584..3855
                     /gene="serS"
                     /locus_tag="IW781_002925"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1183888.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1282 a          673 c          784 g         1159 t
ORIGIN      
        1 aatcaagctt cgcaagtggt tcttttttaa cgactcagtt atcttatcat gtcatttcca
       61 acatgtcaac aacttatttt ttattattta ataagttatt ggtgttcttt tgaacaaata
      121 ataatatatc aacatcacat gtgaaagtca ataagaaaat gcttatttat caacatttct
      181 agcttacatg atcattggtt agttctggtc ctttttcgtt cgtctttata actccgagag
      241 acgtaaaatc cttttgtttt cagtttaatg ttcatgttct tgtcttattc acaccaacta
      301 gatgtctcac cttctaaaat tttccttaac aaagtgctgc ttttatctat ttctgctttt
      361 ttttgctaga ataatagtaa caatatgact ctggaggatt tatttatgaa aattttgatt
      421 gctgacgatg ataaagaaat cgttgaatta ttaagtatct atgttcacaa tgaggggtat
      481 gaagcagtaa aggcttatga tgggaaagaa gcattatcta aaatcagaac aatccctgat
      541 attgaattac ttattttaga tatcatgatg cctgagatgg atgggatgca agtagtgaaa
      601 gagctacgaa aagaatccca aataccaatc attatgctga cagcaaaaac tactgatatg
      661 gataagatca aaggactggt cgctggtgca gatgattatg tgaccaagcc atttaatccg
      721 ttagaagtaa tggctagagt aaagtcttta ttacggcgta caaaaatgca gattgcccca
      781 gatcagcctg atgtattaga agtaaatgca ttaatcatca ataaagactc tcatgaagtt
      841 aaaacaatcg atgggaaaga aatccagtta acagctttgg aattcggtat cctctacttg
      901 cttgctagtc atcctaatcg agtatttagc gcggatgaaa tttttgagcg tgtttggaaa
      961 caagaaagta ttgtctctgc taaaactgtc atggttcacg ttagtcattt aagagataag
     1021 attgaagaag caactggtgg tgaaaaagtc attcaaaccg tttggggagt gggctataaa
     1081 attgacgcaa gatagtaaac gagcaactga aaaacgccgt cggatcaatt tgacttcaaa
     1141 agaaatcagc gaattgttag cggaaggaat catcacgatt atccttttgc ttctattaaa
     1201 tgtttctatt ctggttgtta tcagttcggt gatcaacagt tcaccttctt tgacgaatgc
     1261 gatttgggat tcaaagaata tttttgcaga acgattgaac actgatttgt tctggaacgg
     1321 ccggaatttt attattccct ttttctttct attagatatt ggtgttttgt attggcgctt
     1381 gattcgaaga tatcgccaaa tgcaattgcg gcatatcatc agcgaacttc actacatcgc
     1441 aaacggaaat tatgatcatc gtatcccttt tgaactgagt ggtgacttaa gccgagtggt
     1501 aacaagtatc aatggattgg tggatagtac cgtagcagcg attgaagacg aacgtaaaat
     1561 cgaaaaatca aaagatgaat tgatcacgaa tgtcagtcat gatatccgta ccccactcac
     1621 gtccatcatt ggatacctag gactgataga agatggccag tatcactcag aagaagatct
     1681 tttaaagtat acgcatactg cttatatcaa agcaaagcag atgaaatctt tagtggacga
     1741 cttatttgaa tacactaagg tacgccagcc tgctgtcccg gtgaattttt ctgcctttga
     1801 catgatccag ttgatcgaac agttagcagc tgattttgag ttggaagctt ctaaaaaaaa
     1861 tatccaaata cttgttcaat caaaagtaga ctcccttatc atggatggcg ataccgaaaa
     1921 acttgtacgt gtcttcaata atcttttgac gaatgcgttg aaatacggaa aaggagcaac
     1981 gaagatcgta atcgaagtag aacgtatcgg ttcagaagtt gttgcaacag tcaaaaataa
     2041 cggagcaatg atcccacaac aggcaatcga taatctattc gaccgttttt atcgtgtaga
     2101 agaatctcgt tcacaagcaa ctggaggaac tggacttggt ttagcaatcg ctcaaagcat
     2161 tgtggctttg cacggcggat acatttatgc aaaatcagat aagcaatgga cttctttcat
     2221 catgcacctt ccaatcaaga aaaatgaaaa attacctatt gatacacagg aagttattga
     2281 cgaaagctga aatattcgct attcttatta caattattag aataaatgaa aaacacagaa
     2341 cccagtacac tttattcttt ttcagagagc tgatggcagg tgtaaatcag caaaggataa
     2401 tttggaagtt ccagttcgtt tacgtgccct ttcgaaataa gaaaggtcgg ttcaacagcc
     2461 gttatgtgtt caacaagtgc agctgacgct gaacttgggt ggtaccgcga atttcagact
     2521 gattcgtccc aagaataatg ggatggatca gtcttttttt attttcaaaa aggaggaaat
     2581 catatgttag atgtaaaaat gatgcgacaa aatattgaag aagtacaaga aaaattgatg
     2641 actcggggcg taaaaaaaga aacattagct gaattcatgg aactagatga aaatcgtcgt
     2701 aagctactag tcaaaagtga agaattaaaa aaatatcgaa atgatgtttc tgctgagatc
     2761 gcccaattga agcgtgcaaa agaagatgct tctgctaaaa tcgctgaaat gaaagaagtc
     2821 ggtggcaaca tcaaagcatt ggatactgaa attgctgaag tggatgaaaa attgaaacaa
     2881 attgcaacta ctttgccaaa ccttccccat gattctgtgc cagttggaac agatgaagag
     2941 gacaatgtag aaatccgtcg ttggagcgaa ccacgtacat ttgcttttga accaaaacca
     3001 cactgggaaa ttgcagaaaa tcttgatatt ttagattttg aacgtggcgc aaaagtagcc
     3061 ggtagccgtt ttgtctatta caaaggatta ggtgctcgat tagaacgtgc tgtctacaac
     3121 ttcatgcttg atcagcatat ttacgaacac ggctatactg aaatgatcac tccttatatc
     3181 gtcaacagta aagcgatgtt tggtacggga caatttccaa aattcaaaga agatgttttc
     3241 caattggaga atactgatct aacattaatc ccaacagctg aagtaccatt aactaactat
     3301 tataatgatg aaattttaga tggtgcagat ttacctattt atttcactgc actaagccct
     3361 gcttttcgtt ctgaagccgg aagtgctggt cgggatacac gaggactgat tcgtcttcac
     3421 caattcaata aagtagaaat ggtcaaattt tcggatgtag agcattctta cgaagaattg
     3481 gaaaaaatga cagctgatgc agaagatatc ttgaaaaaat tgcatttgcc ttatcgtgtg
     3541 atggccttat caactggaga catgggcttt tctgcagcta aaacttatga cttagaagta
     3601 tggatacctg cacaagatac ttatcgtgaa atcagttctt gttctaactg tgaagatttc
     3661 caagcacgcc gcgcaatgat ccgttatcgc gataatgatg gaaaaatcca ctatactcat
     3721 actctgaatg gttcaggact tgcagttggc cgtacagtag ctgccatttt ggaaaactac
     3781 caaaatgaag atggttctgt cacagttcca gaagcgctcg ttccttatat ggggaatctg
     3841 aaagttatta aataaagagg ctgtgaaaaa gccgcttagt ttcgagtatt aagaaaaa
//
LOCUS       DACEOM010000143         3553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEOM010000143 DACEOM010000000
VERSION     DACEOM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.151"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2232)
                     /locus_tag="IW781_002926"
     CDS             complement(<1..2232)
                     /locus_tag="IW781_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183889.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="IW781_002927"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="IW781_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1010 a          782 c          551 g         1210 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttc
//
LOCUS       DACEOM010000144         3480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEOM010000144 DACEOM010000000
VERSION     DACEOM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.152"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..999
                     /locus_tag="IW781_002928"
                     /pseudo
     CDS             1..999
                     /locus_tag="IW781_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1100..1672
                     /locus_tag="IW781_002929"
     CDS             1100..1672
                     /locus_tag="IW781_002929"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1183890.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGW
                     CYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAR
                     EVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYI
                     EGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(2145..2717)
                     /locus_tag="IW781_002930"
     CDS             complement(2145..2717)
                     /locus_tag="IW781_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1183891.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2733..3338)
                     /locus_tag="IW781_002931"
     CDS             complement(2733..3338)
                     /locus_tag="IW781_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ1183892.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1140 a          673 c          583 g         1084 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtcaaaa aaactgttgc cattccatca tttcaaaaga
     1141 gaatctctta aatcaggact tttctactga aacgatctgt gagaaatggg cagctgacat
     1201 tacgtacatt cctactaaga aaaatggttg gtgttactta tcttcaatca tggatttaca
     1261 cacgaaaaga attattagtt atacattttc aaaacgaatg actgtggatt gtgtcattca
     1321 aacattgaat aaagcaaaaa tacattatca cattccagaa ggaatgattc tacacactga
     1381 cttgggcagc caatacacag caagagaagt ggaacaatgg cttaaaacca acaaaataag
     1441 gcattcttat agtagaaaag gaacacctta tgataatgct ggaatcgaat ctttccatgc
     1501 ctcattgaaa aaagaagaag tctacacgac tagctactca gattttgaag aagcaaatcg
     1561 agcgctattt agctacattg aaggatttta taaccgaaat cgaatccata gctcgattca
     1621 ctatctaacc ccacaggaat ttgaagaatt agcaaaagaa aaaatggctt aaccgtctct
     1681 actaaaatgt gtccaagata ttgactcaaa tccagtaata tcacaatagg aatgctgttt
     1741 attatattag cagcagtgct gatttttatg atagttattt ggttcttaaa tgaaagatct
     1801 aaagacaatc aacaccgtag ggatatggag ataaaatcaa tagagtcaat agattattta
     1861 tcgataataa cactaaaaaa gccacgttcc taaacgtgac ttacgctgaa cgatatgtgt
     1921 tgtttggtag acgaacaaat cgttcattat aaatatatga tggtatccta atatagtaag
     1981 cgtggctagc atacctttaa ttactttacc ttttacagtt accatacaca ttttttgagc
     2041 agaaatcaag taaataaaaa aatattatat ttcacaacat gctaatagac tcatatctag
     2101 aacgcattaa gtatttacct gtctggttcg ctgagacttc ttttttaact ttcttcaatt
     2161 ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg ttatagcttc aacttcctta
     2221 tacgatttac ctgaagtaag cagttcatat gcgtgcctct tcttcggtgt gattgttgct
     2281 ttcggacgtc cttctctgaa attaggatta ttctttcttg caaacatttt tccttcttgg
     2341 gtacgttcca caatcatatc acgttccatt tctgctacac tcaacaacgt ccgtgtcacc
     2401 attcgcccca tggacgtatt ttcaatcgtt cctagattca aaaccttgat cgttacgtta
     2461 tcatcgagta aaggttcaat gatctctaat gcttctctcg tgttacgagc aaatcgatca
     2521 agcttcgtaa caactatttc atctccaggc ttcagaccag ccaacagctg attgaatttt
     2581 ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa tattcttctt ctctacgccg
     2641 ttctcaagca actgttgaat ttgttctttt gtactttgag aagcggttga gacacgtgca
     2701 tatccatatt tcgtcatttg gtccacctac ttttacaaat catctatcgt atactcagaa
     2761 tacccttcat agaaatagct gacatcgatt cctttcatat ataactcgcg atcgtcaatt
     2821 ttatcagtca gagcattgga aattagatag cgtatttcca aatcgtttat gggacttcgc
     2881 tccattgcag aaagatagtc tgctttatcg attaagttcc aatctacaac tttttgcaat
     2941 tcttccttta aaatgagatc cagccaaatt ctagtacttc ggccatttcc ttctcgaaat
     3001 gggtgagcga tattcatctc cacatatttc ttgataatct cttcaaaaga agtttgtggc
     3061 atctgatcaa tatgctgtaa cgaatgttct aaatacataa ctggtgcaaa tcgaaaattt
     3121 ccttttgcaa tattgaccga tcggattttt ccggcaaaat cgtaaatatc cgaaaataga
     3181 aattcatgaa tttcagataa acctttaaat gtaccaactt cgatttcatt tatttttcca
     3241 caatcgtaca attctttcgc tttttgttta ctgagttttt cttcaacctt agccaactca
     3301 acttgattgg ttaatcccaa tttattctct aaaatcatgc gaaatctcct taactttatg
     3361 acataagttt atgaaacact ctcaacccca ttatatcgcg attttgttta tgtgtcaaaa
     3421 actattaagt ttctgacccc cgatagattt ttcaagtgtc tctattttgc acagtctagt
//
LOCUS       DACEOM010000145         3442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEOM010000145 DACEOM010000000
VERSION     DACEOM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.153"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            382..1422
                     /locus_tag="IW781_002932"
     CDS             382..1422
                     /locus_tag="IW781_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1183893.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            2269..2601
                     /locus_tag="IW781_002933"
     CDS             2269..2601
                     /locus_tag="IW781_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1183894.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3220..>3442
                     /locus_tag="IW781_002934"
     CDS             3220..>3442
                     /locus_tag="IW781_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1183895.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWF"
BASE COUNT         1268 a          466 c          620 g         1088 t
ORIGIN      
        1 cttgtcttat aagaagaata taatatatac tactgaaata aggtagtata tattactagt
       61 aatggtagta tgttttatat gcataaggta gttcttaatt tattttttgt tttgacactg
      121 gtagtactta attatcttta attttaaaac taaattttat tcatacgttc ttctggtagt
      181 atttaattat aaataatatt tatttaaagt agttcttaat gactttaaag gtagtacata
      241 attcatttta tttctgattt taggtagtat ttaattaaaa aacagtttta atgcgtttgc
      301 attttttatt tatctttgct attttcgaaa agagtaggta attttacata aataaaacaa
      361 cgtgaaagta gaggatctac aatgactgat tttaattttt ttaaggcgga tagagtttac
      421 aacgaattat tttatcaatt tccaaaagtc tttattgttt ctgacgaata caaaaaaatg
      481 aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa gattagagat cgcaatcagc
      541 aaacggcaaa tcgatgaaga aggtaatgtt tattttactt atacgacaaa tgaactatgt
      601 agcgtattaa actgccaaaa acaaaaagcg atagcaatca aaaaagagtt ggaatccttt
      661 ggtttattat tacaaaagca gatgggattt aacaaacagt tagggaaaaa taatcctaat
      721 agactatatc tagcagaatt aaaagtctca gaaaatgata tctacttact cgaaaaattt
      781 gatagagaga atagggaaaa cgttgataaa tcagagggta tgaaaatcat acccaccctc
      841 gacgaaaaat cagacgctga atcccttggg gctcaagagg gtatgaaaat cataccgtgc
      901 caaaacgttg ataaatcaga gggtatgaaa atcataccag aacttaataa taatatatta
      961 gacactaata gacacaatat agacactgaa aaagaccgcc tacaagatca attgttgtta
     1021 gacaattttg agacaattat gacaaacgac agcattgcta cgtttatccc tgaacgagta
     1081 ttaaatttaa taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac tgtccagact
     1141 attcataatg caaagaaaaa agccgaaaca gaaagtggta tttcaataat ttttgaagaa
     1201 ctcgatagtt attatgtcaa tgcagaacaa gaattataca cgacactgtt aaaagcctat
     1261 caaaaattaa aaaccgaaaa agttgaaaat atccagaacc tgatttttgt ctatgtaaaa
     1321 aattggttta tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg tttgaattct
     1381 gaaagcttcc caaaaactat tacgaaagac tggttagagt gaaatttcac tgacaaattg
     1441 attgttgctt catggagtag cattaaaatt aaaaattttt tattatttta attatattac
     1501 aaatgttaca aagaaatagc tattttaata gaatttatga gttaaaatat tatgataaaa
     1561 atcacaaacg tagcttttta cgtcataagt gttaatagtt aataattaat ttccaacaca
     1621 aaaaagagaa caaacgtttg catgttgctg ttttttagtt ataataatac gcgaagagag
     1681 gtaatttcat tttttaaaaa agctaaaaac cttgatatta cttcttttca ggcaatacga
     1741 atattattcg cttacatttt tttgtataaa tttagttgtt ttaaatttaa ttgtctgcta
     1801 cgcttgtgat gaattaattt tcgttctgca cttttggacg ataaatttgg acaattgaaa
     1861 atacaaaaaa aagaaaggcc ctgtgagact cggaaggctg gtaaccttcg tcgacgtcca
     1921 caggtggcat taagacaacc tttaatccct taatgcgttt acctttcagt gttacggtat
     1981 tacccgcaac ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg
     2041 ttaaggttaa aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa
     2101 tgtccgtgtg gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga
     2161 tgcgttccct cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa
     2221 caagaaaaac ttattaaaaa tatttaaagt aagtgggtgg attattttat gtcatataca
     2281 ggaattttga gtctcgaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa
     2341 atcacgaaga aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata
     2401 atccagaaag acaaagtctc agaggaaatg atttattacg ctggtaaaca aaagcaaata
     2461 attttaaaag atcctatccc tttaaaagaa ctatatccaa caatcaaaca tgtttatgac
     2521 caaaacggtg tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg
     2581 atggggcgtt taatcagctg aattgatcat tctcataact tgtattttat cgtttttcgc
     2641 ttgcttgaaa atattgtcga gacttctaaa agaacttaga attactagaa aaataaggag
     2701 tagacttgat ctatcaacaa ttcctttcgg ggggtgacgg ggtaggtttt tacgattggt
     2761 aacgtaaaaa attggtcaat cgagtaaatg cccttgtttg gatagtggtg aaatgaattt
     2821 gtgtaaataa tatgcacaga gtaaacaaga gggaatttag aagttagaca agttagaata
     2881 gcgcctagat taatcatgag cagctactaa gggatctagg ctttgacgca aactcatgga
     2941 ataaggagta ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca
     3001 gtacatgtag atacttgcga ggacgttggt cattggatca atcgagcatt taccgatttc
     3061 tactagggtt tctcgtttcg gacgccataa acgagtcgat attttttatg tatttttaag
     3121 taaacgaaaa acgataaaat atgaacgagt cctgaataaa gtgaaaaaat tgatataaaa
     3181 catagagaaa atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat
     3241 atacgattgg gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt
     3301 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     3361 gatttatact atcaaaaatt gactgataat ccaattggat tcggcttgct acctgaagat
     3421 gatgaaggta atgaatggtt ta
//
LOCUS       DACEOM010000146         3403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEOM010000146 DACEOM010000000
VERSION     DACEOM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.154"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(74..1072)
                     /locus_tag="IW781_002935"
     CDS             complement(74..1072)
                     /locus_tag="IW781_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1183896.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1147..1443)
                     /locus_tag="IW781_002936"
     CDS             complement(1147..1443)
                     /locus_tag="IW781_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1183897.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1436..1729)
                     /locus_tag="IW781_002937"
     CDS             complement(1436..1729)
                     /locus_tag="IW781_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183898.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1875..2426)
                     /locus_tag="IW781_002938"
     CDS             complement(1875..2426)
                     /locus_tag="IW781_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ1183899.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2600..2899)
                     /locus_tag="IW781_002939"
     CDS             complement(2600..2899)
                     /locus_tag="IW781_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ1183900.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2926..3192)
                     /locus_tag="IW781_002940"
     CDS             complement(2926..3192)
                     /locus_tag="IW781_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1183901.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
BASE COUNT          993 a          669 c          461 g         1280 t
ORIGIN      
        1 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
       61 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
      121 ttcttccatc tgtataaaca cgaaattgtt ttccaccagc catggtcgtg tttccatcat
      181 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
      241 tatattcatt tgtgctaatt aaattagtat ttgatacatt tttatcagga gaatcataca
      301 accataaagc tttattattt agcacagatt ctttagcagc ttcccaactt gaaattcctg
      361 aactacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
      421 gtgtttgagg agtttgtcca ttcacaatat cactgaaacc ttgttttaac tgttgatttt
      481 gagtttcgtt aaagtacaga taattatttt cattaccttg attttgttgc gttaaataaa
      541 ctttaggttg tccattttga aaaccgaaaa agtaaacatg ctgccctaaa tattctccag
      601 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
      661 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatcgcca ttaatcacgg
      721 cttgtggtaa aggagtgcca tacagactta cattattctg aagattgtag cttttatatt
      781 cttgacctaa tgtctttccc cattgtgtga caaaagtttc taatttggaa gctttattcg
      841 tatcccacaa agtattctta acctcttcag aagaagattc agtgattgaa cttgcacttg
      901 tttccacaga ttgcttcatt tctgttgtag tttgttcagt tgattgcgtt ttactttttt
      961 tagtctcaat tgttgtatca gtttctatag tcgctttagt cctcgtattc tcagatgtac
     1021 aaccagtgaa taaaattccc attcctatta gtaataacag ccattttttc attatatttc
     1081 cccctttcaa tttctccctc acgtacttat tctatcagac aattaattga tctaaatggt
     1141 aatcttttaa tcgattcttt cccaataatg tcgatagccc gtatggcagc ggtctcttcc
     1201 ttgaggactc tcagatggat taaaactagt agaaatttcc cttccacatc ctctacagcg
     1261 ataagttctc attatttttg ggctattagt attatttttc ttagttagag ctatgagtcc
     1321 aactatgatt accccaactg tcttaattac ttttccttta tctatattcg gatgcgcaag
     1381 catatattta tataaccaat atccaccgta aattaaacca cctaatacaa tcagcttagc
     1441 catgtactcg tctcctttct ttttgtttaa ttactttttg aatccatctt gcagagaaac
     1501 cataaacttc tgtcaattga cggatatcta cagttttttc ctgttctatc atgttttcga
     1561 ttctttttct aactaactca cgatcgtaca ttttcattgg aaaagaaatc tgttgccctc
     1621 gaaaagcaag ataaaattga tagagcaatt ctgaatccag ttccatttcc tcaaaaaagt
     1681 cattcatact tttataagag ttactcaaac aagtttcatc aatctccatc tacacacccc
     1741 ctgttatttt ttacataatg aaaatagcat attactgggt acattgataa ttcatataac
     1801 atgtatcatc acatgtattt ttaaagtcaa aaagcatgta aaactactta taagtaacat
     1861 gagaaaataa aatgttattt ttcttgaaat aataattgac tatctgccaa ttgccattca
     1921 ttacgaacat gaatacttag tgggtgggat aacttacctt ctgcatttat caatactttg
     1981 gtcatcttct tttgtttcgc actcaattga tcgtaaatag tctttgcttg ttcataagcc
     2041 acttgactcg tcgctgtaat cgaaccacaa gcaaaaataa tggtattgat tttcttttct
     2101 gtaaaaacag tcgttaatat ttccaaagat tgttcatcat ttcctcgaag aaatacccga
     2161 ggacttttcg cttttgtgta cgaaaaaaga ttcacagcaa taaaaccagt aaatcctaat
     2221 tgtcggatat tcttttcgat taataacata ctcaaatcat tcgtaaatgg ttctgtatct
     2281 gttggacgaa tagtaatgac tactgctaat ttatcttccg cttcttttcc tttgatcgcc
     2341 cattgtcgtt tgaaaacata gcgatgggta tgatgaggat ctcgccaaac ttctgtttgt
     2401 gtggtcacta attctttttc aatcatctct ttttctccta tgctattttt tgttcaagca
     2461 cttcaattac cattgccgtt ataagtgtca atactagttg aaaatatttc atattctttc
     2521 tccttttttc ttcagaattt gatgaatcgc ggtaaggata tcttctaaac atttcgcaat
     2581 cattctcaaa taatcgatct tattcatccg ttcctccaat ctgactccaa accaatcgtc
     2641 cttctaccag taacccacga acaaattctc gctggctggt aatatctgta atgaaataaa
     2701 ctacatagtc ttttccatct tctggtgcgc aataaatata aagatgtgtc atcactaaac
     2761 gtggtagatg aacatcttca ggaagcatac ccatttcaat ttcctctttt tccatcgtaa
     2821 cggattcata gccccactct tcagaagcag tgaccagctg tgcacacttt tccagtactt
     2881 cgatagccgt ttctaacaag tcctttcgtt cctttctttt agatactagt attcttctgg
     2941 taataacatc gtttgatact cacccgaatc aatggcccat attttttgag acactgattt
     3001 caaacgaagc ttaggtgaaa tcagatactc ttttctttct tccggttctt cttgacgatg
     3061 aaccaacttt tgatttcctt tttccgtaga aaattcaaaa atttgaagat agtctaaagg
     3121 aaattgcttt tgaacgtttt gatcgattag attccaacat aaagcttgta attccaccgg
     3181 caaactgtcc attactccct ttgtgacata acgttatcct ttattttgaa acatttcttt
     3241 tccctccttt gccataaaaa tagcccaatc ttctattaaa gaagattggg cttcatggat
     3301 ataatatata tttgtaaata aagttaattc agttataatt atcaaaacta cacaattatt
     3361 aaagtttttc atatctcttt gtttagtaag tttttctaaa tct
//
LOCUS       DACEOM010000147         3316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEOM010000147 DACEOM010000000
VERSION     DACEOM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.155"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(42..746)
                     /locus_tag="IW781_002941"
     CDS             complement(42..746)
                     /locus_tag="IW781_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183902.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(933..1178)
                     /locus_tag="IW781_002942"
     CDS             complement(933..1178)
                     /locus_tag="IW781_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183903.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1195..2046)
                     /locus_tag="IW781_002943"
     CDS             complement(1195..2046)
                     /locus_tag="IW781_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1183904.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2212..3216
                     /locus_tag="IW781_002944"
     CDS             2212..3216
                     /locus_tag="IW781_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1183905.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1026 a          577 c          642 g         1071 t
ORIGIN      
        1 ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc agtaatcgct
       61 gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta tttccgttcg
      121 agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc taatttacga
      181 ggttcatcag catttttttc attaataact acttcatgtt cattttgttc tgtgtaaaat
      241 ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc attcgtatca
      301 gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc ttctggcaca
      361 ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc atcgtttttc
      421 aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg gaatcgttcg
      481 aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt gtcatcgatt
      541 ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt taagatttga
      601 cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa gctctcttct
      661 tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa ttgatttaat
      721 aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc tttcgattat
      781 aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat acgcaaatta
      841 caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa tggaacggat
      901 tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa ggcaaaaaca
      961 ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat tagcgaatga
     1021 tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct tgcaggaaaa
     1081 tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt ctgggtaact
     1141 tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt cgacttaaaa
     1201 ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat cgatttgttt
     1261 catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga ttctgcttgg
     1321 tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca aagcaacttg
     1381 tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat cgaagatttt
     1441 accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg catactcaac
     1501 tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg gcataatttt
     1561 tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac cgattgcttt
     1621 gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat ttgcttgttc
     1681 ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat ctaaataatc
     1741 tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat agctgtggtt
     1801 tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac tttccttaat
     1861 cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat ggcgataacc
     1921 tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg tttgccatgt
     1981 gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc ttgttaatga
     2041 attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca tttttcgaaa
     2101 aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat gattttgatt
     2161 ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa aatggatatc
     2221 tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc agtcttttca
     2281 caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa gaagatccaa
     2341 aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat gatcgttgaa
     2401 tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt tcagcggtgg
     2461 ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt tgtcttgtat
     2521 gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga ggcttacact
     2581 cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa tcgagcaatt
     2641 ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac tgattggacc
     2701 tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta tttttctgga
     2761 agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt gatccaaaaa
     2821 gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat ggctgatttg
     2881 aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga gatgattgcg
     2941 gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga agtacaagag
     3001 caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc tgagaaaaaa
     3061 tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc agtagatgta
     3121 tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat atccgtgatg
     3181 atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata ttaggtgttc
     3241 aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa gacgaatata
     3301 acaaaaaaag agcgtg
//
LOCUS       DACEOM010000148         3219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEOM010000148 DACEOM010000000
VERSION     DACEOM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.156"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..1438
                     /locus_tag="IW781_002945"
     CDS             50..1438
                     /locus_tag="IW781_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1183906.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3219
                     /locus_tag="IW781_002946"
     CDS             1776..>3219
                     /locus_tag="IW781_002946"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1183907.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaag
//
LOCUS       DACEOM010000149         3144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEOM010000149 DACEOM010000000
VERSION     DACEOM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.157"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            88..984
                     /locus_tag="IW781_002947"
     CDS             88..984
                     /locus_tag="IW781_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183908.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            1033..1407
                     /locus_tag="IW781_002948"
                     /pseudo
     CDS             1033..1407
                     /locus_tag="IW781_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1606..1830
                     /locus_tag="IW781_002949"
                     /pseudo
     CDS             1606..1830
                     /locus_tag="IW781_002949"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1888..2415
                     /locus_tag="IW781_002950"
                     /pseudo
     CDS             1888..2415
                     /locus_tag="IW781_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            2429..>3144
                     /locus_tag="IW781_002951"
     CDS             2429..>3144
                     /locus_tag="IW781_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1183909.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKE"
BASE COUNT         1239 a          451 c          563 g          891 t
ORIGIN      
        1 cctagttttt atgaatgacc gttttgtaaa tctgattgaa atagatttat atgatttcgg
       61 tatatgatat acattaggag gtgtcgaatg gaaataaatc tttttgaggc tggtaatcga
      121 ataaagcaaa tacgagaaaa acatggatat actatggcag acttaggtaa actggttgat
      181 gccaatagtc ctagcacaat taacaattgg gaaaagggaa ataacttacc taatagaaag
      241 cgtcttgaaa aaattgcact attaggtggt acttctgttg aatggataaa gtatggagat
      301 ttttccgatt atgtttatcg cttgacacaa aatatagagg aaaaaattct taaagagtac
      361 ggagagacta cactgaacaa ctaccgtgaa gctcttctag atgaacttac gattgaaaaa
      421 atcacatacg aagaagatac aaagatatta gcagctgccg aaaaaatcaa tacacaaaat
      481 atttttcagg aggtgaagta catgtattct cctgatgaag attatcgaaa tatagaagtc
      541 aaaccagatt taggactgac tgatgacaac aaaaatatta aaatgattgg acaacgtata
      601 aggaaaataa gaaaaaaaga gaatattaca ttagaagaat tcggcaaact attctctcct
      661 acagctgata aagctgttat aagcaactgg gaaaatggta aaaaccttcc taactcagaa
      721 agaattaaaa agattgcaga aattggcgga gtttctgaac tttatcttat gacgggtgtc
      781 gattcaagcg tagcagaatc aatgtatttt ctatctgaag tagcattgga tgctttggaa
      841 cgtctgtcaa ttgaggaagt caatagaata attgaaagct tcgccggtta tcttaatgtg
      901 atttcaaaaa ttgatgatcc agataagcga gaaatatctc tcaatgctat ttgtcaacta
      961 tcacttctcg ataaaatggg ttgattcaca aaaaatattg aaagattact ttactcacga
     1021 ggaggcttag ctatgaaacc taatctatcg ttaactagtg aacgtattag aaaaattatc
     1081 gaatccaaag gtctgacatt agaacgattc ggtgatttaa ttggcgacac accaataagt
     1141 tctgttaata attggcttag aggcgtgtct ttacccaata aaaataggct tttaacaatt
     1201 gcaaatattg gaaacactag tgtagactgg attaaatggg gaactttgga agaatacata
     1261 gcttcatacc ttatagattc aggttatgag ctatatatta aagactttcc agagatacct
     1321 tacaaagtct ttaaagacat tcaagaaaaa tatagtgata cgttttcact agataaagac
     1381 tatgagttat tgaatcctat aataaaattt actatagggg gcgttacttt gaatagaata
     1441 aaattttata ttgattccag taaaaagacc attaaacaaa ttagtacaga aactggtatt
     1501 aactatacaa ctcttacgaa ctattaccgt ggtgagcgaa atcctagaaa tactgaaaca
     1561 tggaatatcc ttgctgatta tttccatgtt cctgtaagtt atttgatggg attagacgaa
     1621 acaaatgttg aactcaaaaa ccttaatatt ggaaacaaca tcaaaaatat tagaaaaagt
     1681 aaaaatatga cacaaactga ttttgctgag ttaatgggtc tatcacgatc atatataggg
     1741 gatttagaaa acaacaggag taatcccagt ataaaaacac tggaagctat tgcaagagca
     1801 ttggacgttc cgatagtagc attaatcaac gattcttcta ctgataatat agtagagccg
     1861 tatgaaagtt tgacagccga aaatatcctt cttaaacaag aaaatgagga attaaaatat
     1921 gaggtagaaa gcttacgaaa aagattgtat gaaatccaac aagtagtaaa tcaaggagct
     1981 gaaaaaatga ttcccaatct aaaagacgta agtaaaagaa tcaaatatat cagaaaaaaa
     2041 ttaggtatga ctatggagca atttggttct catttaagca actcacctaa aagtacaatt
     2101 gctacttggg aaagtggtag aaatatacct tctcagaaaa agttacagct gattgctata
     2161 gtaggagaaa ccaccactga ctggattaaa tggggaactt tagaagagta tataacttca
     2221 taccttatcg gtataggata tgagctatat attaaagact ttccagagat acctcacaaa
     2281 gtctttaaag acattcaaga aaaatatagt gatacgtttt cgttagataa agactatgaa
     2341 ttattggatt ctataataaa aaatatattt acaaaatatt actcaaaaga atttgaagta
     2401 acccaaattc cataagagag gtgattgtat gaagccaaat ccttcagaag caggaaaacg
     2461 aatcaaacaa ttacgccttt catgtggttt tactatggaa gaacttggaa ggaaaataga
     2521 taattcacca cgtgcgacca tatccaactg ggaacgtggg accaatttac caaatccaca
     2581 aaagctgaaa ttattatcaa ctatcactaa ttcaacaatt gactggatta aatggggaac
     2641 tttagaagaa tatataactt cataccttat cgatataggg tatgagctat atattaagga
     2701 ctttccagaa atacctcaca aagtctttaa agacattcaa gaaagatata gtaatacgtt
     2761 ttcgctaaat aaagactatg agttattgaa tcctataata aaaaatatat ttacaaaata
     2821 ttactcaaaa gattttgaag attatcgaga catagaagtt aaaccagacc ctaaaaaggt
     2881 agggagaaaa attcgttcta ttagaaaaaa attaggactt actatgcagg aatttgggta
     2941 cgaggtttct aattctccta gaagcactgt gtcaacatgg gaacatggtg gtaacttacc
     3001 gaacaaagcc aaactaaaaa aaattgcaga cattgcgaat tgtagtgtag aagatttgct
     3061 ttttgatgaa ggctttattt ctgaaaaatc ccaagttaaa ttgataaatg aactcaaaga
     3121 agaaaattca aagctaaaag aaaa
//
LOCUS       DACEOM010000150         2952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEOM010000150 DACEOM010000000
VERSION     DACEOM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.158"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            89..406
                     /locus_tag="IW781_002952"
     CDS             89..406
                     /locus_tag="IW781_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1183910.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="IW781_002953"
     CDS             complement(497..862)
                     /locus_tag="IW781_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183911.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="IW781_002954"
     CDS             1070..1552
                     /locus_tag="IW781_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183912.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /gene="pstS"
                     /locus_tag="IW781_002955"
     CDS             1749..2639
                     /gene="pstS"
                     /locus_tag="IW781_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1183913.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          918 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tg
//
LOCUS       DACEOM010000151         2896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEOM010000151 DACEOM010000000
VERSION     DACEOM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.159"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            77..859
                     /locus_tag="IW781_002956"
     CDS             77..859
                     /locus_tag="IW781_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183914.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPGGIEDDSF
                     VEMMVFADHDLRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTASVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNKSAGMTLIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNDVKITKIEGVK"
     gene            859..>2896
                     /locus_tag="IW781_002957"
     CDS             859..>2896
                     /locus_tag="IW781_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1183915.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNEVLAKVEAVSKNVSSAQNDLTILEDRIDQTNQQIGDLGKLKKMYSNSIDFGG
                     YDYSGNPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGK
                     TYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTAN
                     VNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAP
                     LGENIANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSG
                     DYRYGNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGD
                     TRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFAD
                     GIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGV
                     PLDDEVPDEFIARIVHGMLVGSSGGNSVAKAMYVRKDLGTIVTNSDFVVNEWFTF"
BASE COUNT          983 a          520 c          642 g          751 t
ORIGIN      
        1 acaagaaaag tgacaagcaa aggcgtaggg cgcgcgagag acttcactta taactattaa
       61 ggcggtgaac aattaaatgt tagattttca aagcaaacca aatatttttg aagaaatgag
      121 ttatgaagag gctgtgaaat ggttattgcg tcaagcggct atccattacg acggctcgga
      181 tcacgacgcg cacgtcctag ctactgaaag caacgcgggc tttgctacac cagaaacagt
      241 tatgcaagcg cgtgggcgtt ggttacgcga ttataagttg ccacaaaaat atccgaacat
      301 cttggatatt ccacccggaa aatacgcaac caaagccgga tggggtgcag acaaccccgg
      361 cgggattgaa gatgatagtt ttgttgaaat gatggtattc gcggatcacg atttgagaaa
      421 attaatcgtc gcttttgctc gttatagcgg cgaaatttac atcaaaatga cacataacag
      481 cgaaccggta gagggttata actcgttagg ttggcggcgc gtttacactg cttctgttct
      541 ttttgaagga gaattaagaa aaggacaatc agtcaatctg ccagatgata ctttccgtta
      601 ccaaacgctt cgtatccact acacagacgg tgacggagac tttgtagaag aagtgaaacg
      661 tcaaagatac gcgcggatta caaaagctaa tttatggaac aagagtgcag gaatgacgtt
      721 gatcgagttt gaactgacaa tcgaagcgcg aaagataacg atgtcaaacg gcagagcttt
      781 agatatttca tccggcaatg tttccaatcc cgcaatgagc aacgacgtga aaataactaa
      841 gattgaaggt gtgaaataat ggcgcatgtc atcaaaaaag gctctatcaa agtacctaca
      901 cagccgaaag actatgattt gcaagcaacg gggcttgtat ttaaatcata cgataatcaa
      961 atagcgttag agttcaacgt cgaacaacag aacggcacac cggcggactt gctaggagct
     1021 aacttgcgct tgttgatgtt tatctatgat gaagtagatg gaatgatcac gaaagagcca
     1081 attcctttta tcacgaaaaa cctcatcact gaaagcttct tgaatggaca ggtcgtatat
     1141 atcttgccag aagcgatgaa agcttataac ggtatggtgg aagcttatgt ttacatcgag
     1201 tatccagacg gatcaacaag tgataactta ggcttcacct tccgtatgaa gcgttcagca
     1261 atcgacggac tagcgcaaga taaagcagac tactttattg cagactttca acaattactt
     1321 gatggagtca aacaagaagc aacagatgca gtaaatgagg tactagcaaa ggttgaggct
     1381 gtttctaaaa atgttagttc agcgcaaaat gatctaacta tacttgaaga ccgtattgat
     1441 caaacgaacc agcaaatcgg cgatctcggc aagctgaaaa agatgtacag taacagcatc
     1501 gacttcgggg gctatgatta tagtgggaat ccgaatttaa tgtccaaact aaaatcgagc
     1561 gattttaacg ttggttacca cgggtcacta acttcggata acgaaaagct acattttact
     1621 tctgatggta caggaagcat tattatgttt acgcgtatta atacacctca gcttgctagt
     1681 gggaaaacct atactctgag tgcgaaagtt cgatttgatg aaggaactac aggagctatt
     1741 gataaattac gtttggtgta tcgtacatca ccaggagaaa agatattatt ggaagcaaat
     1801 agtacaaata ttacaacaga tgatgtaggg aaagaaataa caatcaaagg tacagctaac
     1861 gttaattatc aaatcacaaa tttagatcga ttttatatga gtattagctt tgttgacagg
     1921 gataaaataa atggcggatt taagttgtac gacatcaaaa tcgaagaagg ctcaacagcc
     1981 accccatatc agccaaactt acttgatgac ccttactggc taggtaaagc gcctttgggt
     2041 gagaatattg ctaataagtc tgttacgttt ccaatcaaat ctagcgccta cagcctatac
     2101 caagctaaca tggaagaaga atttgtttta ggtcaaacct atacaattac catgaaggca
     2161 accaaaccac ctatccaaac gttcattgtt tacaacgagg atagtggaga ttatagatat
     2221 ggtaacttag agccagtaga agggttagtt gatacatggg gtctgacttt tacaccgcag
     2281 aaggttggag ttaactaccc caaacggtta actattatcc agtatcccca atcaacaaca
     2341 ggtgcatgtc aaattgactg gctcaagatc gaaaaaggcg acacacgaac cccgaatatt
     2401 agtcagttta aatactttgg tgaaggcttg aaagacagta acaatcccaa tgattacagt
     2461 tgggatgtca cacctgaata tactgaaaaa ggcttgaatg atgctgttaa tgtgtatgat
     2521 cctcagagag ttgaaggttt gaaaaacttt gccgatggta ttcaaatagc aggagataaa
     2581 gtgatcagtg aaaatgattg cactgtttat acattaacta aagacaacag tcaatcgttt
     2641 atcgatgggt atgcaacatt tattaaacac ggaaaagaag ttattgtaaa tggtacagta
     2701 aagttcaaaa aagcttatgc ttttggtgta ccacttgatg atgaagtccc agatgaattt
     2761 attgcaagaa ttgttcatgg catgttagtg ggatcttctg gcggaaatag cgttgcaaaa
     2821 gcaatgtatg tacgaaaaga tttaggaaca attgtgacaa atagcgattt tgttgtaaac
     2881 gaatggttta cattca
//
LOCUS       DACEOM010000152         2778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEOM010000152 DACEOM010000000
VERSION     DACEOM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.160"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            150..245
                     /locus_tag="IW781_002958"
     CDS             150..245
                     /locus_tag="IW781_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1183916.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(547..792)
                     /locus_tag="IW781_002959"
     CDS             complement(547..792)
                     /locus_tag="IW781_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183917.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1137..1421
                     /locus_tag="IW781_002960"
     CDS             1137..1421
                     /locus_tag="IW781_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183918.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1424..1840
                     /locus_tag="IW781_002961"
     CDS             1424..1840
                     /locus_tag="IW781_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183919.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1977..2696)
                     /locus_tag="IW781_002962"
     CDS             complement(1977..2696)
                     /locus_tag="IW781_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1183920.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
BASE COUNT          927 a          508 c          420 g          923 t
ORIGIN      
        1 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
       61 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      121 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      181 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      241 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      301 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      361 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      421 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
      481 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
      541 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
      601 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
      661 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
      721 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
      781 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
      841 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
      901 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
      961 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1021 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1081 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1141 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1201 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1261 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1321 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1381 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1441 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     1501 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     1561 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     1621 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     1681 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     1741 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     1801 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     1861 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     1921 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     1981 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2041 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2101 ttgaagtatt tttttagttc tcgtaatgca ggatttaata ctttttgatc aatgttaccc
     2161 atttgatagt attctgggat atccattaag ctcctaaact cttcaatacg aactacataa
     2221 taaccagtag aacggtactg cataagcagt cggaatagag tttttgcata cgtagaacgc
     2281 aacgacgtga aagcagataa ttcaaatcta ctaaattcag ctgttaaacc attaataata
     2341 tattctaggt ccggattaac actgacttca acaaactttt ttgttttatt gatttcaaaa
     2401 ccagtaaaca aaacaaagta gcgaatagaa tcctcatctt cagtacggta agtaagatta
     2461 agcatatctt tatagagctt ttctaaatca ccgacaaagg cttttgtagc agtcatctta
     2521 taatcactta actcttttaa ttcctcgaaa gaaaatctaa cttttttcaa accacgatct
     2581 ttcatacgag cgcataatgc aaaaaataaa tccatctgct tagcattgaa atttttcagc
     2641 ggtactaaat tcaattcatt ttgatatttg actgttaatt ccgttttttc atccattcct
     2701 ttcttccccc cttacaaata aataataacc tttaacaacc aactcgtaaa taggttggtt
     2761 gttattatac aaaaggat
//
LOCUS       DACEOM010000153         2710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEOM010000153 DACEOM010000000
VERSION     DACEOM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.161"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            15..380
                     /locus_tag="IW781_002963"
     CDS             15..380
                     /locus_tag="IW781_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183921.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            397..1008
                     /locus_tag="IW781_002964"
     CDS             397..1008
                     /locus_tag="IW781_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAQ1183922.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            1036..2697
                     /locus_tag="IW781_002965"
     CDS             1036..2697
                     /locus_tag="IW781_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1183923.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPDVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
BASE COUNT          867 a          421 c          572 g          850 t
ORIGIN      
        1 aggtggtgaa gttaatgact gcagaagtat gtacaactac ttgtatacat aaagaaaaag
       61 tagctagtgt taaaaataat ttaaaagata aagacctttc aactttactt gtattaggaa
      121 aatgttttag cgattcttct aggattaaaa ttttctatgc tttagaaacg tataaggaga
      181 tgtgcgtttg tgatttagca gcgatactta atgctagtat tgctacaacg tcacaccatt
      241 tacgtttttt gaaaaagaac ggaatggcga aatcacgtca atatggaaag gtagtttatt
      301 actctttagc gaatgaagag attctttccg ctgtgcaggc ttttctaaag ttgtcagaaa
      361 atagttctat gaaagcttag tttggaggta gttattttgt tacaaaacat tttatcagca
      421 ctcgctgtat atatttctac cagtattgat tatttattta tcttgctgat tattttttct
      481 caaaaccata ctaaaaaagg tctccgtcag attttttttg gtcaatatct tggaacagga
      541 atcttggtag ccataagttt gtttgcagca tatgtgctaa attttattcc tcaagattgg
      601 atcattggtc ttctgggact aattccgatt tatttaggga ttcgtgttgc ttttgtgggc
      661 gaagaggagg aagaagaagg ggaagtcgtt gagaagctag gatctagagg aacgaatcgt
      721 ttgttttgga cagtttcctt aataacgatt gcttctggtg gcgataattt agggatttat
      781 attcctttct ttacctcgtt atctttttca gaaattgtca cttctttaat cgtctttgct
      841 atctctgttg cggttctttg ctatattagt tataaatttg cgaaaatttc ttttgtctct
      901 gaaacactag aaaaatatga acgaatcatt gtacctatcg tatttattgg attagggatt
      961 tttattatga tagaaaatgg aaccatccaa actatattag gtttgtagag atcttattag
     1021 taaaggaagc aaacgatgag tgaaaataaa aagtaccatg aatggatatt agaaggaatc
     1081 gactgtgcaa attgtgcaga caaagtagaa agaggcgtcg ccaaagttgc aggtgttgcc
     1141 aatagtagtg tgaactatat gacagaaacc ttgagtttcg aagtgacaga ggaaaaagaa
     1201 tatcttgttt tatccaatgt gcaacaaaaa gtaaaaaaat tggagcctga tatcacttta
     1261 aaaaataaag tggacggtag gctgattgaa ataaatccgg cattaatcaa gaaaagtaat
     1321 ttaaaagacg ataaaaaaaa ctacgaatgg atattagaag gaatcgattg tgcaaactgt
     1381 gctgccaaag tggaaagagg agttgctaca gttcccgacg ttacaaacag cagcgtcaat
     1441 tttatgacag agacactgag cttcgaggta gcaggtgaaa ataaaactga cgtgttggct
     1501 agtgtaaaac aaacagtagg aactttagaa cctgacgttg ttctgcgaaa aaaatcggac
     1561 ggtacattga ttgaacatgg aactgactct aataaaaatt cgacttctaa caaatcgatt
     1621 tctgataaag atgagacaaa ccaaaagcag acgaatagag cttatctggc cagccttctc
     1681 ggaaataaaa ctaaaattac ccttattcgt ttaatactag gattaggaat attagcgaca
     1741 ggaatatttc tccctatgga tggacaagcc tctcttgggt tgttcattct ggcttattta
     1801 atcaccggtt atgacgttgt aggacgtgcc attagaaata ttttccgagg tcaagtattc
     1861 gacgagaact ttctaatgac cattgcgacg ttaagtgcct tctatattca agagtatccc
     1921 gaagctgtag cagtcatgct attttatcaa gtgggagaag tattccaaga tattgctatt
     1981 tcaaaatctc ggcgctccat tgcggaccta atggatattc gacctgatta tgccaattta
     2041 gtcacaggaa acaacgtaga aaaagttgct cctgaagcaa ttaagattgg aaatattatt
     2101 ctcattcgtc cgggcgaaaa agtgccattg gatggaaaag tagtggaagg tgcttctgcg
     2161 atggacactt ctgctttaac aggcgaatcc gttccaagaa gcgtgaagcc ggaagacact
     2221 attttgagcg gatttattaa taaaaacgga ttgctaaaag tagttgtaga aaagcccttt
     2281 tcggagtcta ctgtgaagaa aattttagat ttggtccaaa atgctagtgg ccggaaagca
     2341 cctactgaac aattcattac caaattttct cgctactata ctccaattgt ggttatagct
     2401 gctgccttac tggcaattgt tccgccattg ctagtaccag gcgctacttt taatgaatgg
     2461 atatataggg ccagtatctt tttagttatt tcatgccctt gtgctttagt cgtttcgatt
     2521 ccagttggtt tctttggtgg gattggttct gcttcccgca aggggatttt agtaaaagaa
     2581 agcaatttcc tcgagggttt gaacgatctt aaatacgtgg taatggacaa aacgggtaca
     2641 ttgacaaaag ggttctgttg caaagtttta aatctactat caaataaggt agaataatag
     2701 aaaaagatag
//
LOCUS       DACEOM010000154         2629 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEOM010000154 DACEOM010000000
VERSION     DACEOM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.162"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            89..313
                     /locus_tag="IW781_002966"
     CDS             89..313
                     /locus_tag="IW781_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1183924.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(605..817)
                     /locus_tag="IW781_002967"
     CDS             complement(605..817)
                     /locus_tag="IW781_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183925.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(820..1032)
                     /locus_tag="IW781_002968"
     CDS             complement(820..1032)
                     /locus_tag="IW781_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183926.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1307..1717
                     /locus_tag="IW781_002969"
     CDS             1307..1717
                     /locus_tag="IW781_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1183927.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1730..2182
                     /locus_tag="IW781_002970"
     CDS             1730..2182
                     /locus_tag="IW781_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1183928.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            2273..>2629
                     /locus_tag="IW781_002971"
     CDS             2273..>2629
                     /locus_tag="IW781_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1183929.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDINLLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLK"
BASE COUNT          958 a          410 c          396 g          865 t
ORIGIN      
        1 gtatatgcca tatcacttct cctttcgttt attgaatttc tccatttcta tttgataaaa
       61 tatttctata gaaatggagg tgaaaactat gggaaaaaat caacatgttg tacctaattc
      121 caatggtggt tggaatgtca aaggtgctgg aaattctaaa gcgactgcac atactaatac
      181 aaaatcagaa gcagtaaaaa tcgctagaga gatttctaaa aatcaaggat ctgagctatt
      241 tattcatggc agagatggaa aaattcaaag ccgtgatagt cacggaaacg atccacaccc
      301 accaaaaggt taatcatatt taggagttaa tcgaatacga taaccgtcag caggagttgc
      361 atcggtcaat gtaacttctg ctactttttt cccttcttct gtttcaataa ttaatcttgt
      421 gtaattgttc tcattcaaaa tccctatttt tgattcttcc ttcatgtcat ccctccttcg
      481 tctctcgccg tcaccacctc caagaagagg agttgccaaa atgaaaaaac gtggactttg
      541 gcaaacttga aaggagagca gcaactcctc ttttcagaag tggtgaattt agatttttta
      601 gctattaaca aacagctcta actctaaatc gatttttaga acgccatgtt ctaaactgtt
      661 catataaatc gacatcaatt gttatttctt tggaagtaac tgcgttatac ccctcactaa
      721 aacgtgattt tttaaattcc ttcattcttc tttgataagt actttcagaa tacttatatt
      781 tctctttaaa ctccgctttg ctcaaacgtt gtaccatttt tacgcctcct gttctgatgg
      841 ctgatcatta gaatctactt ctaacttgat atccaaaagt tcttcgattt gttttaaacg
      901 ttccttaggt tttctttttt cgatgaagat atctcgtaaa taagtaattg atatacccat
      961 tcgtcgagct aactctcttt gactccaacc tttctttact aaagcaatta atacttgctg
     1021 ttttaaagtc atgcttcata cccccttatc gaatattttc taatattttt gtctaataag
     1081 tttgacaaaa actgcaaata tgctattatg taagcataca aaatacagcg cacaaaaagc
     1141 gagcttactg atgtctgggg agactattag tttattgctt ttagtctttg tatgccgtaa
     1201 atatcttgct tacaaaacta gtataactgc aaattctcta tttgtaaata gataatttgc
     1261 agaaaaatac tttttttgtc ggtattttga aaggaatgca tatcctatgc tattagaccg
     1321 tataaaaatc ttattaaaag aacgttcaat gacgttggca gaacttgaaa gaaagttaaa
     1381 tttcggaaat ggaagcatgt ctagatggaa taaaagtact ccatctggag ataaaataca
     1441 aaaagttgca gattacttta atgtatcaac tgattatctt ttaggacgta ctgataaacg
     1501 ccattattat gatttaacag aaaaagataa acaagatatt gctttacaag ctgaaaaatt
     1561 acttgaaggc ttgaattcag atgctgaaac aaattattat ggtgaacctt tagacgatga
     1621 agataaggaa aaattatacg atgccattca acttgcactt gccttaacca aagttaaagc
     1681 aaagaaaaaa ttcactccta aaaaatatcg tcaataggtg ggctttggta tgcatttaaa
     1741 caaatactgg atattagaag aaatagaaaa attatttgta gagtttggat ctgatgcgat
     1801 taatccatat aaaataatca aatattctga tatagattta attgaaactg atttagggaa
     1861 taaaacatta ggacaaacga tacacaataa aagatgttat gtgatattaa ttaatcaaaa
     1921 cctatcacct tctatgaaaa gatttgtatt atggcatgaa atagcccacg ttagactcca
     1981 taaaggtatt tgcacagcag cttttagagc aaacaacctt agttgtatga tttatggtat
     2041 agaagctgaa gctaatatgt ttgcaattga aatgttaact agaacaatag atgaacaaga
     2101 tttatctgat atgagtcagt tccaaattat tgaatattta gggcttcctc ataatcttga
     2161 acatttagta ttacaacatt aaaaataaaa aagcccatgc tttcacatgg actctttcct
     2221 catttctgag taaactctac aataattata tcaaagaaat gaggagtttt gaatgttatt
     2281 attgattgga cttattttat taattatagg aatcactaaa ctgtcgaaag ctaacacaat
     2341 aaaaaaagaa gcttatgaat tagaacaaaa aaatgatgaa aaactaaata aattacatga
     2401 tagagaaaga tttatattaa gtaaggaaga aacactatca gatttaaata aacaaattac
     2461 tatgttaaaa aatgatatta atttattaaa aaaagagaaa gaagatttaa atgatgaaat
     2521 aaatcaaaag tatgatgaaa cattaaaaaa tgtctattct tcacttatcg tttcaaatat
     2581 agaagaaact tcatcttctg aaataaaaaa taagctttct ttgttaaaa
//
LOCUS       DACEOM010000155         2484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEOM010000155 DACEOM010000000
VERSION     DACEOM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.163"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1014..1304)
                     /locus_tag="IW781_002972"
     CDS             complement(1014..1304)
                     /locus_tag="IW781_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183930.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="IW781_002973"
     CDS             complement(1512..2204)
                     /locus_tag="IW781_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1183931.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          734 a          458 c          349 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DACEOM010000156         2397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEOM010000156 DACEOM010000000
VERSION     DACEOM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.164"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            206..2068
                     /locus_tag="IW781_002974"
     CDS             206..2068
                     /locus_tag="IW781_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1183932.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="IW781_002975"
     CDS             2068..>2397
                     /locus_tag="IW781_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1183933.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACEOM010000157         2211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEOM010000157 DACEOM010000000
VERSION     DACEOM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.165"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW781_002976"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW781_002976"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1183934.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          588 a          479 c          405 g          739 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtgtat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt a
//
LOCUS       DACEOM010000158         2197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEOM010000158 DACEOM010000000
VERSION     DACEOM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.166"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(423..1715)
                     /locus_tag="IW781_002977"
     CDS             complement(423..1715)
                     /locus_tag="IW781_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1183935.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1989..>2197)
                     /locus_tag="IW781_002978"
     CDS             complement(1989..>2197)
                     /locus_tag="IW781_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183936.1"
                     /translation="NIVIFTDWLGVLFSNLIPILIIGICGIILSILELFESRNTMNRI
                     FACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          721 a          419 c          351 g          706 t
ORIGIN      
        1 gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag ccctcttgta
       61 tgggctactc tgtgatactt attagccatt aaactatttt acccacatag catatatttt
      121 gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa atctaagata
      181 gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac aactttaaaa
      241 aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg tttaatcatt
      301 catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc caaaaactat
      361 gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg gctattttga
      421 aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt acggaaggta
      481 aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg attaagcacc
      541 atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt cgtgcgtaaa
      601 taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt gacttttctg
      661 aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg aatggcacta
      721 ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc tacagcacca
      781 gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc gtttagagaa
      841 gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt acttggaata
      901 tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac cgatggaaag
      961 atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa aaagacatct
     1021 tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt agattctgtt
     1081 tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc aagaatggtg
     1141 tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat acaatcaacg
     1201 aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa ttcaggaagg
     1261 acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata aatagcttcg
     1321 acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag aaccttccct
     1381 tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa ggaagcttcc
     1441 cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt tttagttcca
     1501 tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc ataacctaaa
     1561 tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac atctttcaaa
     1621 gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga ttcagctaac
     1681 ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct tgatcttatt
     1741 gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta cttacacggt
     1801 ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca atgaaattat
     1861 ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt tgatcctatt
     1921 atccatatct ttttcagttt taatattgat acacattata agaaaaagaa acttttggtt
     1981 aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg caatagatta
     2041 acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc aaaaagttct
     2101 aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag attagaaaat
     2161 aacaccccga gccaatctgt gaaaattact atattta
//
LOCUS       DACEOM010000159         2173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEOM010000159 DACEOM010000000
VERSION     DACEOM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.167"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..335
                     /locus_tag="IW781_002979"
     CDS             <1..335
                     /locus_tag="IW781_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1183937.1"
                     /translation="DESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIIL
                     ADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKV
                     NKMNLEVE"
     gene            335..1711
                     /locus_tag="IW781_002980"
     CDS             335..1711
                     /locus_tag="IW781_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1183938.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1841..>2173
                     /locus_tag="IW781_002981"
     CDS             1841..>2173
                     /locus_tag="IW781_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1183939.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          844 a          269 c          399 g          661 t
ORIGIN      
        1 cagatgaaag tatcttgttt gaactaggtt tagataaaaa acaaataaaa agaaatgtta
       61 tgaaattatc tggtggacag caacaaagag tagctattgc tagagcattg gtatcagatg
      121 ctccaataat actagctgat gagcctactg gtaatctaga cagtgttact gctggagaaa
      181 taattaatat attaaagaca ttagctaaag atagaaataa atgtgtaata gttgtaacac
      241 atagtaagga agtagcagat tctgcggata tcattttaga actaagtggt aaaaagttga
      301 aaaaagtaaa taaaatgaat ttggaggttg aataatgata aaaaatgcat ttgcttatgt
      361 aactagaaaa agtttaaaat cattaattat catattagtt attttatcta tgtcaacttt
      421 aagtctcatt agtttatcta ttaaagatgc tacggacaga gcttcaaaag aaacatttgc
      481 taatataaca aatagttttt ctatggagat aaatagacaa gtaaatccag gaacacctag
      541 aggtggggga aatgtaaaag gtgaagatat taaaaagata tctcaaacag atagtataga
      601 ctcctatgta aaaagaataa acagtgttgc agatttagtt gattatgata ttattgaaac
      661 tcaagagact cttgcgaatc aatcacctga aagagcaaag aattttaaaa gaacagttat
      721 gttaactggg gttaacgact caacaaaaga aacaaaattt gtatcagaag catataaatt
      781 agtagaagga aagcatttag aaaatgaaga taaaaataaa atcttaatgc ataaagattt
      841 agctgaaaaa aataacctta aagttggaga taagataaaa ataaaatcta atttatttga
      901 tgctgataat gagaaaggtg cagatgaaac agtagaagta gaaattaaag gtctattcga
      961 tgggcataat agcggtgtag taagtgcagc acaagaacta tacgaaaata cattaatcac
     1021 tgatattcat acagctgcta aagtttatgg taataccgaa gatacagctg tataccaaga
     1081 tgcaacattc tttgtaaaag gtgataagaa tcttgatagt gtaataaaag atcttgggaa
     1141 attagatata aattggagag aatataattt gattaaaagt tcatcaaatt accctgcatt
     1201 acaacaatct atatcaggta tttactcaat ttcaaataaa ttattttttg gctcattaat
     1261 atttgcagga gttgtagttt cattattatt attcttatgg atgaatgcta gaaagaaaga
     1321 aatagcagta ttactatcat taggtatatc aaaattagaa atttttggtc aattcctaat
     1381 tgagatggta tttatatcaa tccccgcatt cgttggttcc tatttcttag ctcaatatac
     1441 agctgacaag cttgggaata atatattgaa taaggttact ggtgatatag ctaaacaaat
     1501 agctaaacaa tctgcatcta gccaattagg tggtggggca gaagctgaag gatttaacaa
     1561 gacattatca agtttagata tcaatgtatt acctaaattc atgatttatg tagttatatt
     1621 tatgagttta gtacttcttg tatcattgat tatttcttca ttcaatatat taagaaggaa
     1681 tccaaaagaa ttattaattg acaataatta aaattttcgg tgctttttag caccaaaaat
     1741 gagagtgtaa tataaaccgt gtaagtaacc atcaatccta ggcgggtggt tgcttcacgg
     1801 tttttatttt tctaaaataa gatcaagagg tgattacacg atggctagaa agaaaagaaa
     1861 tcctgatgct gagaagttag ctgaatccat tttgaatact tatcaacccg aatctgtaga
     1921 ggatatgcaa gatgttttaa aagatgtgtt tggtccctta tttgaaaaaa tgcttcaagg
     1981 agaattgaat aaccatttag ggtatgacgc ccattctaaa gagccaaaga aacatgacaa
     2041 tcgacgaaac ggttatggca ataagacgct taaaacgagc tttggtaaag taaacattga
     2101 tgttcccaga gaccgggagg cttcttttga acctgagttg attcctaata gaaaacgaga
     2161 tgtctccgac atc
//
LOCUS       DACEOM010000160         2136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEOM010000160 DACEOM010000000
VERSION     DACEOM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.168"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            95..313
                     /locus_tag="IW781_002982"
                     /pseudo
     CDS             95..313
                     /locus_tag="IW781_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            573..827
                     /locus_tag="IW781_002983"
     CDS             573..827
                     /locus_tag="IW781_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1183940.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            828..1145
                     /locus_tag="IW781_002984"
     CDS             828..1145
                     /locus_tag="IW781_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1183941.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1704..>2136
                     /locus_tag="IW781_002985"
     CDS             1704..>2136
                     /locus_tag="IW781_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183942.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT          754 a          307 c          391 g          684 t
ORIGIN      
        1 tagatatttt ttgctacata gagttacttt caatattttt taacgtaatt aggcagaagt
       61 cactcatcct ctataggttt gtgatgaaat tgctattgat ccatttaagt aagagcaaaa
      121 aactggaggt ttatcaggta attggtctaa tggatttaag tatgcggtca ccgtgtatag
      181 agttgcttat gagattcaag aaaataaagt tattccagta ttgctatatg gaacacataa
      241 caatttttat gaattagccg agaacactcg caactttatt cgtgtagatg aatcggcttc
      301 ggttgagtta taactttagc tatatcaatc aaccgaccga catatgttaa atctatatat
      361 aataaaaata cctacttaaa tggatgttaa cggaagtgta tggtgagtag ttcactcgta
      421 ttttgatatc gtttgtatat gttttcagcg ttctaagtgg cttattttgt ccaataataa
      481 tatggagtgg aatcccttaa gaaacaccgt tggcaattgt cgttataaca tgttataatt
      541 tatttatcaa aaactaaaag aagtgtgata acatgggaaa agaactaaag gttagaaaga
      601 tcggcaatag cgtgggagtg atccttccaa gctctcttgg attaaaaagc ggagacacta
      661 tccaagccaa acaagaaggc aacttattca tattagatac tactcaaata gcaaaagaac
      721 acgatcgcaa attgattgaa gaaagtttcc aagactttga gaaaggttta acagtttcag
      781 aaattgaaat ggtaaaagct ttcggaaaat acggctggag tgaatagatg aaagagtaca
      841 cgctagttta ttctaaaagt ttccagacca gccttgaaac gataatagaa gaatgggaaa
      901 atgagcttgc tctttctcca gccagcataa aaagatttgt aggtgcgatc cagaaggctt
      961 tagaactaac caaaaatttt ccaactatgc atgaggaagt ttccgaaatc tatggaatgg
     1021 atatcccaac atatcggatc gtgatcggca aaagctatgc cttattttat cggatcgacc
     1081 atgaaaaaaa agagatactt gtaggacgta ttttccaatc taaacaaatg aaactaggct
     1141 tttaacaata attaggcaaa agtcgctcat cctctacagg tgggtgatta atgccgctcg
     1201 atatggttgt taaccgtcgg ttactcgaag attgatatat aagacttagt acctttttat
     1261 tttgctccat gttatggccg aaggtctaaa gatacaatca ttagtctaga gaaaaattaa
     1321 gaaccgtagg aactacgggg atcgcttggt gaataagaga cactgctatt agtgaaaaaa
     1381 taagatatca agtacgctct attcccaaaa atttcgtgac tttactcatg agaggttcaa
     1441 gtagtatatt tttaatcaaa attgacgtaa cttttaataa atactaccta aataaggtag
     1501 tatttatttt tgtgttttaa aagaggaagt ccaatatatc tatgggaaac ttcttaaata
     1561 ttatgttggt acatgaaaat atacatccct gctagactat ttacaacatt ttactttgta
     1621 cgtattcttg ttttaaaagg aatgggataa aatataggta caataactat ttgatgctaa
     1681 atataattaa tggaggagag atagtgaaca tacttaatga aaaaagatat gtatggtttg
     1741 ctggtaaaaa atatattact actttcaaaa gattatccga gttaatgcag cagactgtta
     1801 aaagtgctat gcctgaagga tacaagttta attttgttca atcttcagta tctactgaaa
     1861 gtatctattt ttcaattctt tctgagagta ggctttttca ttttagaata agttctcatg
     1921 taaagaaaac tgatgttgat atatatacgt tccgtacaaa taattataaa aatttaaatg
     1981 atttgagtag aagtatcagt gaatatctca aggggaatag ctatctcaag attcgatttt
     2041 cacatatatt aattttacta ccatatttga atgagaattt caccctaatt caagtagata
     2101 agtctttcat tgcctcaggt aatcaaggaa atatga
//
LOCUS       DACEOM010000161         2103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEOM010000161 DACEOM010000000
VERSION     DACEOM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.169"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            156..374
                     /locus_tag="IW781_002986"
                     /pseudo
     CDS             156..374
                     /locus_tag="IW781_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            869..1468
                     /locus_tag="IW781_002987"
     CDS             869..1468
                     /locus_tag="IW781_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1183943.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1532..>2103
                     /locus_tag="IW781_002988"
     CDS             1532..>2103
                     /locus_tag="IW781_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183944.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          758 a          326 c          392 g          627 t
ORIGIN      
        1 tttaatttaa tattttgcct caaattagcc acacttttta tagaactaag aggaaaaatg
       61 aagttttctt cctattaata tagttgtttg aaatcttaaa tatagcgttt tacgatgtaa
      121 tgggaagtat ggtgaataaa tcaactatgt tcgtacgaag catttactca actaatttaa
      181 tcgaatcttt caataaacaa atcaagaaat acagccaccg caaggaacag ttccaaaatg
      241 aagagtcgat ggaacgtttc ttagtctcgt cttttgatac ttacaaccaa aaattcctag
      301 gtcgcagtca taaaggtttc caacaggccg aaggcgagct cgaacaaatg ctgagtcaac
      361 cgatggagaa ttagaaacaa tctacagtag ggaagaacca ttgacacaaa attattgacg
      421 ctcccaacta aaagtgtcaa aataatgatg aaatcattta gttcttataa attagaaaag
      481 agaaaataaa tattccatat ctactttatt ggatccgtaa catgactaaa ttctgaatac
      541 tttttaaagc ttatcatttg gtaaaactga atataagtta attatataga ctgaaaattt
      601 tgaattttca gaatagaata acttaaaagt actgacaaaa ttttttaaag gggcaatatt
      661 aatgttctga gataatcaaa tcctaccgaa aatatcaaaa atccaatgtg acacaatcag
      721 attgtgtctc attcgaggga atcacacaat cccacgtggc tctaggcaga attgaatttt
      781 tgcttggggc tttttcctta tattgatttt gaatgagact tattatatgt tataatgcaa
      841 ttaagtatca ttggagaggg cgaagaaaat gaagacggta gaacctattc gagacaaaaa
      901 aaagatcgat gcgatgaagg caatcttggc ttcgggaaaa tatggccaac gaaatttggt
      961 tcttttttcg attgggatca acaccgccta tcgcatttct gatttgcgtc aattgaagct
     1021 ggccgacgtg ctggaaattt cacgcggtcg cgtggtggtc aaagatcgat tagcgatgaa
     1081 ggaacaaaaa acagctaaac ataattcggt ctatatttcc aacaaattgc gaaaaacgat
     1141 tttggagtac gttcaacagg aatttcctga tcaactcctt cagcaagatt ttgacggtta
     1201 tttgttccct agtcgaaaag ggacgaatga accgctcaac cgccaaagtc tgtggcgaat
     1261 cattcatgat gctggcacgg caatcggatt gaaagacatt ggcccacatt ccatgcgaaa
     1321 aacgttcggt tactttctct ataaacaggg gaccaaaaca gaaatcattc agtccttgtt
     1381 gaaccattct tctcagcgcg aaacattgcg gtatattggt attacacaag aagacaaaga
     1441 tacagctgta aaaagcttag atttataata taaaagttaa gtttaaacat ggatagtttt
     1501 aaattagtag attgagtgtt gggaggaaag aatgagtaca ttatatatta taggaaacgg
     1561 ttttgattta agtcatggtt taaaaactag atacactgat ttttcaaaat gggttgaaaa
     1621 aagtcatagt gaaatattcg ataaaataga cacaatgata aatgaaaata atgaggtgag
     1681 aagtacagct gatgataaga taggttggtt taattttgaa agtagtttgc atgagttaat
     1741 tcaaagtcca gtctttgaag attatgtaga agagtctaag gattatctca taggttatgg
     1801 acatgaagat tggtcagatt ctgcccatca tgattttcaa aatatggttt atgattatgt
     1861 tgtttctcta gaagaattac aacaactttt caaggaatgg atacgcaaag aaatacagcc
     1921 gacaatagaa cataaaattc caaaatacca attttcgtcc gatgtactat tctttacatt
     1981 caattataca gatgttttgg aaattctata caagcaaccg aaagaaaaca tatatcatat
     2041 tcatggcagt tatgacgata taataatcgg ccactccgag aattttgaca actttatagg
     2101 aga
//
LOCUS       DACEOM010000162         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEOM010000162 DACEOM010000000
VERSION     DACEOM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.170"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW781_002989"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW781_002989"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1183945.1"
                     /translation="MVNKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IW781_002990"
     CDS             complement(1431..1823)
                     /locus_tag="IW781_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1183946.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          579 a          450 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttatttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACEOM010000163         2029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEOM010000163 DACEOM010000000
VERSION     DACEOM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.171"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            265..597
                     /locus_tag="IW781_002991"
     CDS             265..597
                     /locus_tag="IW781_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183947.1"
                     /translation="MNKEIENLIEELKKECDKENIGLSLSLVNEETFGMIAAGPANLA
                     VMGALLQGKEYIEIAAGNCHCEECKKIREVINAVDDEVSSTQHTFVINNKEDFADVMT
                     RIFKGEFE"
     gene            836..1777
                     /locus_tag="IW781_002992"
     CDS             836..1777
                     /locus_tag="IW781_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1183948.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIARDQELIDTITERLVEFWETNVVEGIEPVIDGSEATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESAIRQVDNEIISELGKRETSIGITQKNIISWKLVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            1779..>2029
                     /locus_tag="IW781_002993"
     CDS             1779..>2029
                     /locus_tag="IW781_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1183949.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNL"
BASE COUNT          750 a          301 c          442 g          536 t
ORIGIN      
        1 caagctgaac taattgcttc tgaacttgag gatttgagaa tgctagaaat aaaaaatagg
       61 gaggctagat tgtgacttta caagagcatc aacgattgat gcaagagtta aatcaggaat
      121 atcacaaaga tgtgaaacct gagttgctag gtaatcaatg gcgtgaacgc cagaagaagt
      181 ggcgagaaat aaaaaaagac caactaacga tgagggtcgc taattggtaa taaataaaaa
      241 tatatatcag gagaattata acatatgaat aaagaaattg aaaatcttat tgaagaattg
      301 aagaaagaat gcgacaaaga aaatattggt ctttctttat cattagtaaa tgaggaaaca
      361 tttggaatga tagctgctgg tccagctaat cttgctgtta tgggtgcttt attgcaagga
      421 aaagaatata tagaaatcgc tgcaggcaat tgccattgtg aagagtgtaa gaaaataaga
      481 gaagtcatta atgcggttga tgatgaagtt tcttccacac aacatacttt tgttattaat
      541 aacaaggaag atttcgctga tgtgatgacc cgtattttca agggggaatt cgaatgatta
      601 aaatcaatac gctttggatt gcactatttg tctttatgct tgtagtaatc tcaaacgcta
      661 atttgtttgt aggctgttta tttgcaggaa tgattacttt gctaagcttg ttagaagatt
      721 ttacaaagaa agaaggcgct aggcatgaac gagttagcaa atcttgacaa ctatttaact
      781 gaccctgatt atgctaaacc accttatgaa gcgccaattg atgaggagga tgaagatgag
      841 taaatctacc ttagaaatga gccatcaaga atggctcgaa gaccgcaaaa gaggtattgg
      901 tggctcagat gttgcaaccg tacttggatt aaacaaatac aaatctcctt atcaattgtg
      961 gcttgagaaa acgggtcaaa ttgaattgaa agatttagaa agcgaaccag cttattgggg
     1021 taatgtttta gaagaagtgg ttgctaaaga gtttcaagaa cggacaggta aaaaggttcg
     1081 cagaagaaac caagtctttg aacatccatt acatccattt ttaagagcaa atattgatcg
     1141 ggaagtagtg ggagaaaatg ccattctgga atgtaaaaca gccaatcaat ttctcggaaa
     1201 agagtgggaa ggtgaagagg taccgcttag ctatctctgt caagttcagc attacatgaa
     1261 cgttttaaac aaagactatt gttatatcgc tgttttgatc ggtggacaaa aattcatttg
     1321 gaagcgaatt gcaagagatc aagaattaat cgatacaatt acagaacgct tggttgaatt
     1381 ttgggaaaca aatgtcgttg aaggtatcga gcctgtaatt gacggaagtg aagcgactgc
     1441 tgacttctta aaagaaaaat atgcagatgt agaagaaaat caaacagctc taccatcacg
     1501 ttttgatgaa cttatcgagc aaaaaagaga actcaagcgg acgaaaaaag aaattgaatc
     1561 agctatccgt caagtagaca acgagattat cagcgagcta ggtaaacgtg aaaccagtat
     1621 cggtatcact caaaaaaaca tcatcagttg gaaacttgtc cgtacgagac gtatgaactc
     1681 gaagaaacta gcagagaaat atccagatgt cgcaaatgat gaagagattt ataacgttac
     1741 tgaatcaaga aggctaaccg aaaaggagat caaataatat ggcaacaaat gaatcgttaa
     1801 aaaatcaatt ggcagaaaag ccacagaaac aagttgcacc aggacagtta gggcttaaag
     1861 ctctaatgaa tacaccaaca atgagaaaga aatttgaaga agtacttcat gacaatgcta
     1921 atgcttttat gtcgaatgtt atgactcttg tatctaatga cagttatctt gcagatagtg
     1981 aaccgatgtc tatcatgagt ggtgcgttaa ctgctgcaac attaaatct
//
LOCUS       DACEOM010000164         2027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEOM010000164 DACEOM010000000
VERSION     DACEOM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.172"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..995)
                     /locus_tag="IW781_002994"
     CDS             complement(<1..995)
                     /locus_tag="IW781_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1183950.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(1013..1567)
                     /gene="srlA"
                     /locus_tag="IW781_002995"
     CDS             complement(1013..1567)
                     /gene="srlA"
                     /locus_tag="IW781_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1183951.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            complement(1555..2025)
                     /locus_tag="IW781_002996"
     CDS             complement(1555..2025)
                     /locus_tag="IW781_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1183952.1"
                     /translation="MVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAIGKRPGKI
                     RSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPSVRAEDRL
                     TQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKRWIS"
BASE COUNT          577 a          457 c          344 g          649 t
ORIGIN      
        1 cagctatata atccaaaact tgctacccaa gcaatgataa ctcgtgggat gccgttaaga
       61 aatcttgaat aaagaactga tggtacacct acttctactg tctcagcttt tgcttcttcc
      121 aaacctagac cgactggaat aaaatcacat gcattttgag tattgatagc aaatagtgcc
      181 ggtaatgcta attggggagg aatatttcct ttcccaatct ctacaccgat caatgttcca
      241 atcacttggc taataactgc ccctggacca agcaaaggag ataaaaatgg cagtgaacaa
      301 ataaagccaa tgattgttaa tccaactgca tttcctgcta aaggtgccat aatgttagca
      361 aatacattac cgatcccaga accttgtatc acaccaatca aaagggaaac gaaagccata
      421 aaagggatca ctgtccggat catcgtatct actgcttctc ttgctgattg attgaatgta
      481 gcaactactt ttcccgcgcc cattccaatt tttgctatcc agttttgctg ctctgctttt
      541 tgctcggtta tctttttaga agtatcatat ttgtactttt tatctggaga tgcagtattt
      601 actgtttctt tgttatcatc agtttccatt agtggctgta tttgttctaa ccctactgct
      661 gaaacataaa tatcttcagt gatatattgt gctaatgggc cactttttcc tgtggaaacg
      721 atattgatgg ttggtatccc ttttttagga taaattccac atctaagagt ccctccacag
      781 tcaacgattg caagcgcgat ttcactatca ggtattgatg ttttgaaccc attgatagac
      841 tccatacctg tcagttcttc tattttatct acgatcattg gcctctcacc tccacctgtg
      901 acatagatga acttgtgttt atcttttgtc ggcctaataa tcaaaggacc tccatacccg
      961 ccgtttcctc ttacgatctt aatatcacca tacatttaaa actctccttt ctctaatcaa
     1021 ctgaaagctc tacttttttg ctaagaacga ttccttgctg tctgcataca aatccagtag
     1081 taaaatcagt tatccaacca ccaataaagt tcatcagcag tcccacaagc aagtatcgga
     1141 tagccagctc catcgaattt agccctaagg tttcgatccc ttgagcaatc cccatccata
     1201 caaacaattc tccaggatta atatggggga atacaccatt gctcgtatga caaaattgag
     1261 cttgagcagc ataatagctt ggtttgtagt attctggaag gaaacgagcc attgtaaatg
     1321 acataggatt tcccaacata aaagcagata agaacggtaa cacaaggtaa cgagtaagcg
     1381 gatttttcgc tgataatttg gctactttcg ttactcggtc ttcacctaaa aaagcaatca
     1441 gcgtattcat tgcaaccatc agcatcaaga caactggtac gattcctgtc atccagctta
     1501 tgaatgtttc tgcacctgtt tggaacagcc tcataaatcc ctcagcaaat tttgttatga
     1561 tatccatcgt ttgtcactcc ttttttcttt gatcgtactt ttccatatcc gaaaattatt
     1621 gagcatagca ataaaaggca atgaaggttg ttcacttttt tcatttcctg cctctattcg
     1681 taagtatact tctctggcat tttctaaagc tgcttgtgtc aaacgatctt ccgctcgaac
     1741 tgagggatgg taacgatcga tgtaatggat atcttcaccc gcatagacag gtaatgaatg
     1801 aaattttgcc aaagatgtaa ccccttgcat ttttctgact tctaaaactt gatcatgatc
     1861 gtctatagct agcataacaa tcgtgcctga acggattttc ccaggtcgtt taccaatcgc
     1921 tacttttcct tttcttcgca gttcgctata tgtttgattg aaatgcttta gttgcttcca
     1981 actgataact gcttgcagca cataagctga agctgcgaaa actatga
//
LOCUS       DACEOM010000165         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEOM010000165 DACEOM010000000
VERSION     DACEOM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.173"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..378
                     /locus_tag="IW781_002997"
     CDS             <1..378
                     /locus_tag="IW781_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ1183953.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IW781_002998"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IW781_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1183954.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IW781_002999"
     CDS             1859..>2003
                     /locus_tag="IW781_002999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1183955.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACEOM010000166         1955 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEOM010000166 DACEOM010000000
VERSION     DACEOM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.174"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            201..1166
                     /locus_tag="IW781_003000"
     CDS             201..1166
                     /locus_tag="IW781_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1183956.1"
                     /translation="MSKNGTIVNEWENLTALSNRRLNVKTFYTPLKIRWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKINQFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKAVVPKEIETWWRLELQ
                     LRRGKATDWHKMVHESLDSFASPHFLPIDTKHTDRIMIAGLMSDHSLWSGLTRPTKYK
                     YRNLLKQESQNDELTNHLRETFSESADNLKQELDTWLLGLDVTEK"
     gene            1366..1568
                     /locus_tag="IW781_003001"
                     /pseudo
     CDS             1366..1568
                     /locus_tag="IW781_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          645 a          293 c          433 g          584 t
ORIGIN      
        1 aaaaaaatag ccgattggtg ttggaagcac ctttcgacta attacggaaa ctatcgcact
       61 agctccgtaa ctcataatca ttatacgaat atttttaggt ttcttcaaga ctagagattc
      121 ttgttaatga attttaaggg ttacgtgaac ttttaaaatt ctttccaaga atctcttttt
      181 tctttgagga ggaacgaaat ttgagtaaaa atggcacgat tgtaaatgag tgggaaaatt
      241 taacagccct atctaacagg cggttaaatg taaaaacttt ttacaccccc ttgaaaatac
      301 ggtggtcaat tgaccgaatt accattgttg gaaaactgaa agaaaatatt tattatcata
      361 ctcaaaatga tgtattgatt ttagactttg aacagttaat gcgactgaat gaaaataatg
      421 gttatgttaa atctgttggt ctaaatggtt ggcaaattgt ggataagcat gatgagaata
      481 ttgcttacat tgaaattttg aaatatcaag aagggcaagg gcgaattgac tttaatccga
      541 ataaaattaa tcagttttta gctagctcga tgaagaattt tatccatgat ttatttttgg
      601 agccgcattt ttcacgtgca gatattgctt gcgatataat tgacgtacca gatgatttca
      661 ttacccaata tcgggtggtt gatcctgtat cctttaaacc aatttatggt cggtctggca
      721 agttagaaac tgcttattgg ggcagtcgtg ctagtgaacg tcaagttcgt atgtacaata
      781 aaaagctgga acaggaacga aaaaaagcag tcgtgccaaa agaaatcgag acttggtggc
      841 gtttagaatt acagttgcgc cgaggaaaag caactgattg gcacaaaatg gttcatgaaa
      901 gtttggatag ttttgctagt ccacactttt taccaataga tacaaaacat acagatagaa
      961 ttatgattgc tggtttaatg tcagaccatt ctttatggtc ggggttaact agaccaacta
     1021 aatacaaata tcgaaatttg ttaaaacaag agagtcagaa tgatgaattg acgaatcatt
     1081 tacgagaaac attttcggaa tcggcagaca atttgaaaca agaattagac acgtggcttt
     1141 tggggttgga cgtgacagag aagtgatacc gaataagaaa agtcagatga gtgagagtgg
     1201 atttgcgtca atgtttagtg acgtaaattc acgataagcg aatattttgc atgaagcaaa
     1261 ataaagcgta aaaggagttg agagcgtgac acgctttcgg ctcctttttt cgttgtcact
     1321 tgtgacagcg aaaaccctcg gagagcgttt ggaattgtga cagccatgtc acaattagga
     1381 aaatgttact ctttgacaaa gagtaacaga aaggtttaca ctctttttat tagcgacagc
     1441 cgaaggaggg ttttaacttg gggttcagta tttcgttcaa aaagggaaca aagaatacaa
     1501 gcatttctca taataatcgt gagttaactg atagccaaaa aatcaatggt ttgtctggta
     1561 gaaaataatt agcagattta caagtgggaa ataaaaagct gaaagaagaa aattgcattt
     1621 caaactacat tttctacaat agctctaaaa cggcatattt tgcgttctga gcggttttag
     1681 cgtttatttc gtttagttat cggcacaatc gttaaaacac gttagaggag cgagaatgga
     1741 gcctgtaagg cgactggtag cggaacggtc gtgttagatt gtgaaagccg atagctgaat
     1801 gaaataaaca gcgggtgcgc ccttccattt cggcaggagg aggctcaagg ggtttgggga
     1861 atgacattcc ccaaggtctt gtgtctttta tactcgggag tatttgtcag tttttgtttt
     1921 ctggtgttat aattatcaaa aatcgaaaaa agagc
//
LOCUS       DACEOM010000167         1899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEOM010000167 DACEOM010000000
VERSION     DACEOM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.175"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..308)
                     /locus_tag="IW781_003002"
     CDS             complement(<1..308)
                     /locus_tag="IW781_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183957.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     "
     gene            complement(283..>1899)
                     /locus_tag="IW781_003003"
     CDS             complement(283..>1899)
                     /locus_tag="IW781_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1183958.1"
                     /translation="IYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISF
                     KQCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQ
                     LVAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGD
                     GGDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGG
                     GNSGGNDNGWNGQYPPGVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANP
                     TADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHA
                     PNGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKD
                     LEIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGE
                     VIVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPS
                     TGEHLHFQFMDEFYPSSSGHFHNARDYIDF"
BASE COUNT          489 a          448 c          358 g          604 t
ORIGIN      
        1 tgccagtcag gctcacttaa ggtgtaaaca agccttgctt gcgctggact gtattcttgt
       61 actttttgtt caacaggttg gttttcgcca aattttgttg tattaataaa aaacggcact
      121 aggccctcga atgtttttag tttgccatgt tccaccactt caacaacgaa cttttgcgtt
      181 aaaacatccc cttgccgaat tcgaaccaaa tttattccgt tgtttggttc ggtggtggat
      241 aggaccattt tatgctgcgt ttctgccatg actatccctc ctttagaaat cgatgtagtc
      301 tcttgcattg tggaaatgac ctgaagaaga tggataaaac tcatccataa attgaaaatg
      361 aagatgttct ccagtggatg gtcctgttgt ccccatcagt ccaatttgct gaccagcagt
      421 taccttctga ccttttgaga cgtctactcg gctttgatgc gcgtatcctg tatacattcc
      481 atcagcgtgt ttaatcactg tccaatttcc ataccagtca aagtaatttg catcacctgc
      541 aacaatcact tcgccatccg ctgaagcaaa aataggtgta ttaggatttc catttacaag
      601 gtcaataccg ttatgaaatt cttgtgctcc tgtgattgga gaagtgcgcc agccaaattc
      661 gctcgtcact gtgattggat ccgcaatcgg cttcatatat ccttttgata caggaatttc
      721 caaatctttg aacttgtcat accattcttg tgaccatgcc gtccgttctg gatgtggatc
      781 acgtggacgt tcaaagttag ccacgaatgc ttgtgctgct gtgttgatat cggtcagatt
      841 catgaattgt gtccatgtgt aaggataagc gctagttgcg atccattgac catttggtgc
      901 atgccacatc aacaatttga actgtgccgt gattgtgtct ggatcatcac tgatgccagc
      961 ctttgtcatt aaattgatca tgtagacacg tccgctagtt gcgccagaag aatcggtcca
     1021 ttgccataca ccataaccga accctggtgc gccattgccc tcatcagcgg ttggattagc
     1081 atctgattct ccttgtgcat ttccaagtaa ggctgcagca gcttgttgtg taaaaccggc
     1141 acctattgcc gttgcccaaa tttgccaata tcgtttatcc cgatcagtag ttactcctgg
     1201 tgggtattgt ccgttccaac cgttatcgtt ccctccggaa ttgcctcctc cattagtgtc
     1261 aatttttgta ccgttaacat agagttcttt tacatctagc cgtccatcta tggttatatt
     1321 tccttctgaa aaattgccgt caccataaag attatatttg cgcttatcag cagtaacatc
     1381 tgctggaatt tgaaaaatag gattccctcg atcgccaccg tccccagcat taatggaaaa
     1441 aatatagttt ggctctttcc agacagcaaa gccgttaatc cctttagaac cataagtaga
     1501 tacaattgag cccaagcgtt ctccatgtac atcatctagt ccagtagact tgacttgttt
     1561 ttcaaaagaa agttctcccc cctctgccac taattggaaa tctttatcgt caaatgtttt
     1621 taaagctacc ccttgcacga ggataccaga cagaatgcct gcgcgaataa aattagcatt
     1681 gaaagttcca tccaacgtcc aagcggtcgt gctatcacca ttgtgtacat cttgaattgt
     1741 ttgccactca ccttttttac attgtttgaa agatatccct gagttatttt ggaccataaa
     1801 aaagcgtgat ctaggaatgt taggtccatc catataaaca gtttcataga tttctctact
     1861 atcactaaca ccagcttcaa ttccatttac ccaataaat
//
LOCUS       DACEOM010000168         1873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEOM010000168 DACEOM010000000
VERSION     DACEOM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.176"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..719)
                     /locus_tag="IW781_003004"
     CDS             complement(<1..719)
                     /locus_tag="IW781_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1183959.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDRPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENA"
     gene            complement(730..927)
                     /locus_tag="IW781_003005"
     CDS             complement(730..927)
                     /locus_tag="IW781_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1183960.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(950..1243)
                     /locus_tag="IW781_003006"
     CDS             complement(950..1243)
                     /locus_tag="IW781_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183961.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRITIHEELKKHDERLDTHAEKLVEHTQQIKTLFREKSR"
     gene            complement(1281..1418)
                     /locus_tag="IW781_003007"
     CDS             complement(1281..1418)
                     /locus_tag="IW781_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1183962.1"
                     /translation="MFSFSDVKMMFDWNCFTADQVRQFVPLCITDEEADKIISKEESA
                     S"
     gene            complement(1420..1866)
                     /locus_tag="IW781_003008"
     CDS             complement(1420..1866)
                     /locus_tag="IW781_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183963.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGLRADGEPL
                     TIADQMFDPKLNQWIVLANALDHNDLNNLKAMYEALEHENDNLKQLNAKLMLNDVAIK
                     QENTTLKEKADSLAQINSKMMLASLQNSKDIAEIKKQLNPESEGGE"
BASE COUNT          508 a          385 c          311 g          669 t
ORIGIN      
        1 tttgcgtttt caatttcccc aaactgatca atatgagcgt agtttgttcc atctgtcagt
       61 gtaggattag ctgcacctgc tctagttgga tcaatgactg gtctatcatc tgaagtagtt
      121 ggattttcat cggtaaatcc atgagctaaa tcatatgcta atttttcttt acttacgccc
      181 atttcagaaa gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat
      241 aaatgtgatt tgattcctgg ttggttataa ggagtgtcca atgtaagcgg aataccgtat
      301 ttcttagcgg aatctctcgc caattcaacg tatgctttat agttcttttc aaaagttgct
      361 ttattatgtg tgtgttgtaa ctcaatctgc acaggactgt tggcattagc atacgaacca
      421 gcaccgtact gtacataacc aggttgtcca acttggtaaa caattccgcc atcacccaca
      481 atgtaagcag tgtaagcact ggtccatgaa cgttgcatat actgcgcttc attgcgtcct
      541 gttgctgttt cattagccgt ttcatgcagt aaaatgtact gattatttgc tatttgtgag
      601 ctaccttcat ttgcgcccaa attaaattca ttattgatcg tataagcaaa accattaatc
      661 ggcaataaaa aaagagccgt taacaggctc atcgcagtaa tagtaatttt ctttttcatt
      721 tgtttcctcc tattttttca aattataagc cgacacacca gtgataacgc ctaaaaatgt
      781 tgctactgca ttgatagtga gtactgtcat atctgttcca ttccatccat aggctttccc
      841 taacgttgct actaacacag aagcagctgg caatactgtt aaaactgtcc atttaatgac
      901 ttgataatac ttatcgggta aaatcatttc ttcttaactc ctttcttttt tacctagatt
      961 tttctctaaa taaagtttta atttgttgcg tgtgttccac caatttttct gcatgtgtat
     1021 ctaatctttc atcatgtttc ttcaattctt catgaatagt aatgcgatca gatttgctcg
     1081 attctaaatc tttagtcagc aaatctaaat tgtgacttac ttttgaaaga gtctcagtaa
     1141 tcttcgagaa agatgcagta attggtttta ttactaataa aatcaaagaa acgatagcgg
     1201 ttattgatcc tgctatcgct ccccattccc ctaaattaat catgtgacaa ctccttgaat
     1261 caaaataaaa agcacatcaa ttaagatgcg ctctcttctt tgctaatgat tttatctgct
     1321 tcttcgtctg taatgcatag tggaacgaat tgtcgaactt ggtcagcagt aaaacaattc
     1381 cagtcaaaca tcattttcac atcgctaaaa ctaaacatac tactcacctc cttctgattc
     1441 tggatttaat tgctttttaa tttctgcaat atccttgctg ttttgaagcg aagcaagcat
     1501 catttttgaa ttgatttgtg ctaaactatc cgctttttct ttcaatgtag tattttcctg
     1561 tttaatcgct acatcgttta gcatgagttt agcatttagc tgttttaggt tgtcgttttc
     1621 atgttccaga gcctcgtaca tcgctttgag attgtttaaa tcgttgtgat ctagtgcgtt
     1681 cgctaaaaca atccattggt tcaatttagg atcaaacatc tgatcagcaa tcgttagcgg
     1741 ttcgccatca gcacgtaatc cttcgagcgg tggctgatcc gtgtaaggaa cggatacaag
     1801 catgtcgtcc aatacttttc ctgcgtactc tccaccagta cgtccgtatt tccaaatgtt
     1861 tttcatttat ttc
//
LOCUS       DACEOM010000169         1863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEOM010000169 DACEOM010000000
VERSION     DACEOM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.177"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            298..594
                     /locus_tag="IW781_003009"
     CDS             298..594
                     /locus_tag="IW781_003009"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1183964.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(517..1397)
                     /locus_tag="IW781_003010"
                     /pseudo
     CDS             complement(517..1397)
                     /locus_tag="IW781_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1473..1628
                     /locus_tag="IW781_003011"
                     /pseudo
     CDS             1473..1628
                     /locus_tag="IW781_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            1647..>1863
                     /locus_tag="IW781_003012"
     CDS             1647..>1863
                     /locus_tag="IW781_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1183965.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKD"
BASE COUNT          578 a          323 c          328 g          634 t
ORIGIN      
        1 ttattctttt cataaatatt gtaaatagaa gtattaaaaa tatatacaaa aagtattatt
       61 tcgagaaaga taaatatttt caaaaacctt ttcataatcc tcaccctttc ttacactgat
      121 aaatcatgcg tctaatattc aaattatact cgatatgctt tagctaatca gccaactctg
      181 tttgtctact atcattatat caatagacaa tatctgctcc cgtctttatg atcgtgccaa
      241 agcaatcaaa agaaaattgc gaatcactta gatcgaaagt ttactcaaac aaaagcattg
      301 tcagcggttg tcacggattt aacttatgtt cgggtcggga caaattgggc ttatatttgt
      361 ttgatgattg atttattcaa tcgtgaaatt attggccatt cttgcggaaa taaaaaagat
      421 gcgcagcttg taaaaagggc cattcaaagc attccttact cacttcaaga aatcgaactc
      481 ttccatacgg atcgtggaaa agaatttgat aaccaaacca ttcagaatct catgaatggt
      541 ttggttatca aattcctttc cacggtctgt atgaaaaagc ttgacgtctg ttaaagaata
      601 cggtatccgt ccaaaggctt cttttaccaa tgaggcatct ttcttctcac cacaggaata
      661 accaataatt tctcgattaa acaaatcaag tattaagcag atataatgcc actttttccc
      721 cacgcgaaca taagtaagat ccgtaacgat ggcttccaat ggctgttctt gtgtaaaggt
      781 ccgatctaat acattcgctg ttttcgcttc attacaagct gttcgttgcc ctttaaaatg
      841 agcgatcgta taggtagatg tcaatccgcg gcgtttcatg attcgaccga ttcggcgccg
      901 actgagttga agcccacgct ttgctaaaca cttcttcaat tttcgggtac cgtaagcctt
      961 tcggtttcga ataaactctt cctgaacgat ctcttctaag tcggactcat tttcgatcgg
     1021 tttcgcttga taatagtagg tttgacgaga aatatttaga attttgcaca tcgctgatat
     1081 ggaatattta tgcttgttgg catcaattac ttgtcttttc gtccgaatat cagcgccgct
     1141 tgctttaaaa tatcattctc catttcgagt tgcttatttt tctttcttag tgcgatcaat
     1201 tctgcttgtt ctggtgttaa gttgtctctt tctttgaatg agcccgttga ttgtgcttgc
     1261 ttcatccatt tatcgaaaga agaaggcgta agctcatact ctcgaataat ttctgcgcga
     1321 ggcttaccag cgagatagag atcgacgatt tgttgtttga attcttttga ataggttctt
     1381 cgttgacgtc ttggcatgaa aaatcctcca gtgtgttttt tattattcta cacaccttat
     1441 tttttctgtc tagtctagtg tagccgattc aggataagga taagtgtatt aattgtggaa
     1501 aatgtaaaaa agtttgccaa atgaataaag attcagttga gaattgcaat catctggagt
     1561 gtatcagatg tggacgatgt aaaaatgctt gtccagtgga tgcaattagt tatggagtaa
     1621 gaaaatagag aggagtagtt aggaaagtga taaaaaatgc tatagcatat attacaagaa
     1681 agaaaaatag aactttaata atttttatca tattaactat agttctttct tgcttgtatt
     1741 cttgtttaac aataatgaaa tcaagcaatg aaatagaaaa ggctttatat gaaagttcta
     1801 attcttcaat atcaataaca aaaaaagatg gtaaatattt taatgttaat caatttaaag
     1861 ata
//
LOCUS       DACEOM010000170         1747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEOM010000170 DACEOM010000000
VERSION     DACEOM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.178"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(191..646)
                     /gene="tnpA"
                     /locus_tag="IW781_003013"
     CDS             complement(191..646)
                     /gene="tnpA"
                     /locus_tag="IW781_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1183966.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            889..1747
                     /locus_tag="IW781_003014"
                     /pseudo
     CDS             889..1747
                     /locus_tag="IW781_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          562 a          345 c          329 g          511 t
ORIGIN      
        1 tgccaaaatt tcatcaccct ttctggacat aagtttctga caccagttaa aatcaagtct
       61 atcagtaacc catacacccg tgaggacggg tgtattttta atccccctag aaggggtcga
      121 cactcgctgc cccttaaaag gggattttta aaagaccgcc aactgctcat cttcactcac
      181 ttacttctct ttatttttta aatggttcta cgtactctct tttacttata atatatcgaa
      241 tgcgatcatc agcttcttgt tcacggatgt atttttcatt tgtcttttga ttcaatccga
      301 ctgtactcac atagtatctc ttcgaccaaa aggatttgtt tccgtattta tatttgagat
      361 ttgcgtgctg ctcgtggatt agtaccgcac tacgaccttt cagatacccc ataaaactgg
      421 atatacatat ttttggtggt actcgaacaa gcatgtgaat atgatctagc attgcatgtg
      481 cttctatgat ttccacatct ttcatttcgc acaacttcct aagtattttt ccaagatctt
      541 ttcgtaattg cccatagatt acttttcgtt gatattttgg tgtaaaaata atatgatact
      601 tacaattcca acgagtatat gctaaacttt tatcgtcatt tgacatgaaa aaactccttt
      661 cgatctgatg agcggttggt agccactcac tagtatatcg aaaggagttt ttatcgctaa
      721 agatacttct tccacgcgta gaaacgcgcg gttttcttgt ttcgcacgct ttacgcacaa
      781 aactgactga agtcattaag aaaataaaag ggtctaaaac ttttaagttt tgtaccgacc
      841 cccaaaagtt agaccaaaaa atctaacttt tggaggtcgg tattttttat ggctaagtat
      901 acttttgaac ttaaattaaa gatcgttcat gattacttgg atggtaaagg cggaagtgat
      961 tatctggcaa agaaatattc cattaaggct ccatcacaag tgaagcgctg gatcaatgcc
     1021 tatcaagaat ttggtgaaga gggtctagta agaaagcgac aaaagaaaaa atattctgtt
     1081 caatttaagc tggatgcgat agagttatat ctaacaagtg agctgtccta tcgtgaagtt
     1141 gccaacacac ttaacatgaa caaccctaat ttgatcgcta gttggatgcg tcaattccgt
     1201 gagggtggga tcgatggact ctcaaaaacg aaaggatgtc cacctatctt gtctaagaaa
     1261 aatgaaccca aaaataagaa aaaatctaca actaaagcaa cgtctaaaga acgtgaacgc
     1321 atcgaggagt tggagaaacg agtccgttcc cttcaaattg aaaatgcctt tttaaaagaa
     1381 ttgaggaagc tgcgaaaaca ggaagcccaa caacgacgaa tgaagcaatc gcacgaatca
     1441 tcgcaagcct ccgaggaccg tttaaattag ttgagcttct agaaacttta aagttcccta
     1501 aggcaatctt tatgtactgg caaaaacgat tggaccgtaa aaatccagat caagagattg
     1561 aagcggaaat gcttaaagtt cgggagaagc acaaggatta tggctgttta cggatgacca
     1621 atgaactaag aaaccgcggt ttctctatca ataaaaagaa agttcagcgc ctgatcaaga
     1681 agctaggaat aaaagtaacg acatacacac gtaaatcacg gcgctacaat tcttatcgag
     1741 gacaagt
//
LOCUS       DACEOM010000171         1736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEOM010000171 DACEOM010000000
VERSION     DACEOM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.179"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(192..1484)
                     /locus_tag="IW781_003015"
     CDS             complement(192..1484)
                     /locus_tag="IW781_003015"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1183967.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          498 a          366 c          303 g          569 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga actttatctt tttagaatgg
     1621 ttattaaaaa agaggttgta acagaagtgg tcagcttcaa gaaataagaa ggaatgtacg
     1681 aaaattgctt ctcaaatttt tgtgaaattt cgacttattt ttcgaagaag ctactt
//
LOCUS       DACEOM010000172         1697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEOM010000172 DACEOM010000000
VERSION     DACEOM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.180"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            158..769
                     /locus_tag="IW781_003016"
     CDS             158..769
                     /locus_tag="IW781_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1183968.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
BASE COUNT          674 a          180 c          343 g          500 t
ORIGIN      
        1 ggttgaattg atagatggac tcaaagaaga aaattcaaag ttaaaagaga agataaaata
       61 ctatgaatgt ttatttgatg gtatagagag tctgctgaac tcacgaaaaa attcctagtt
      121 aattaaacta gagtaaaatt atttgtaggt gttaaatatg ttagttggct atgcacgagt
      181 cagttcagat gatcaaaatt tggaaagaca gcttgaagaa tttagaaaaa ttggtgtcga
      241 aaaagtattt gctgagaaaa aatctggacg taacacagta gaacggccag agttcaataa
      301 aatgttgtca tttgtcagag aaggggatac gcttgttttt gagtcgcttg aacgcttagg
      361 aagaaattct gatgaaatac tagaaacagt atcttttata gataaaaatg gtatgggctt
      421 aatggttttg aatttaccaa ttttagatac aaaatttggt gatccaaact tagaaaaact
      481 tgttagaagc ttagtaatta acattttttc ttggacggct caaaatgaga gggaggaaat
      541 taagagaaaa caaaaacaag gaatagcaat tgcgaagaag aaaggagtct ataaaggaaa
      601 accctacgaa tattcttcta ctgcaaagaa tccacaaaag aggtttatct ataatgaaat
      661 agtaagaatg cttggagaag gtgttccgat taagcgaatt gcagaaaaaa caggaacaaa
      721 tagaaattta gtctatagaa taaagaaaag aataagtgaa tctgattgat tttaagttct
      781 ttgagaaatc aatgttacga gagaaggatt ctaagcctgt ttcaaacttt taaggtgtac
      841 aattatacaa agaagcttct aaacaaaaga attaattttg tggcagtcaa gtaacgacag
      901 aaatagaaaa gaaagaggct atgcgagagt gaatcaacaa gttaattggg ttaatgattt
      961 tgaaaaagaa tttcgactga aattttttgg aaatcctaca gcagaaagta cagaagaaaa
     1021 aaattatgac tggtcagaat tttaggttgc atattcgatt caataagtgt tgaagataat
     1081 agttctctta cgccacagta tacaacgtta gaatatgacg aaaacagatt caatacattt
     1141 ccttgcaata ccattcaaga atcaattgat aagcatgagt gataattgat tatctacagg
     1201 aaggtgtttt ttatgaaact agagatcaaa gaagttgtct gcgattgggg aatttatgta
     1261 gacggagaaa catatccatt tatgattttt aatagtaaag caaatgcaca agagataatg
     1321 cgtatcatgg agttggataa taagcacgaa agatttgatt aagtaaaaaa aatggagaat
     1381 gaatgtgaag cagtggaata ccaaaaaaca tgcgccaata ataagtatat tttaggtgca
     1441 atgggattag gtaaatcagc ttctattaaa caggtctacg aagaatcaca ggagaattgc
     1501 gtatatgaaa attcgaatta aagagtgaga atatgtggga gctaatttat ttagttttat
     1561 acttaatagg aaaatcaact gaatttgtat ataacaataa ggtaatattt atattgttct
     1621 taatagtttg tattatcgta tttgatgtct atgatttact aattcataag aatgaaaata
     1681 gtaatattgg tagaatt
//
LOCUS       DACEOM010000173         1514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEOM010000173 DACEOM010000000
VERSION     DACEOM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.181"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..661
                     /locus_tag="IW781_003017"
     CDS             <1..661
                     /locus_tag="IW781_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAQ1183969.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQ
                     RKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLG
                     Y"
     gene            717..1214
                     /locus_tag="IW781_003018"
     CDS             717..1214
                     /locus_tag="IW781_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1183970.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(1251..>1514)
                     /locus_tag="IW781_003019"
     CDS             complement(1251..>1514)
                     /locus_tag="IW781_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183971.1"
                     /translation="DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVG
                     SDGHYLEHFRFHFDDLFALLKANGVTELAIYQKGKRIMVPLPV"
BASE COUNT          534 a          256 c          267 g          457 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa tccatgaaaa gcttgccctt aagggcatta ctatatccgt
      421 tccgccacgt aaaaacatgg ataaatcaga aaaactagac cacagcttac ttggaaaaca
      481 aagaaaaaca gttgagactg ttttttcttc cttagaaaaa ttaggttgtc aaaatttcaa
      541 ctcacgttct gttaaagggc tagagagtag atttgaaagc atattactgg cttacagtgt
      601 tctattaagt cgagcacaac gacgttttga aggaactttg agatattctt taggatatta
      661 aaattaacta gcaccacggg tgatttatag tatactatgt caaaaggagg agttagatga
      721 acaatcaaat cacactagcg tatttagatt atgatgatct agatgcttta caagttgtaa
      781 gtgttgtcag tttttatgaa tcgtttatcg atggcgcaga tccacttgat atgcaaggat
      841 atttacagac acaattgaca actgaaatat tggcagtaga attggcacag tcaaccagta
      901 aatttattgg tattaaggat catcaaatac tcattggtta tatgaaagtc aatgatgaaa
      961 aagatgctat agagattcaa cggctctatc tgctcaaaga ttatcaaaac aaagggttag
     1021 ggcaacggtt actcgatgaa gcgaatcgtt atgctaaaac gaaacaaaaa cgttatttac
     1081 gtttgactgt ttatgaaaaa aatcatgcag gtattcgttt ttatgaacgt catggattta
     1141 aaaaaatcgg gatcaaacat tttcctttag gaaaacaaga tcgtatttgt ccgattttag
     1201 aaaaagaaat ctaaaaaaac gtcctcactt ttgatgctga ggacgttttt ttagactggt
     1261 aatggaacca taattcgctt tcctttttga taaatcgcta attccgtcac accatttgct
     1321 tttaacagag caaaaagatc atcgaaatgg aaacggaaat gttcaaggta atgcccatct
     1381 gatccaacac taaacaaggt accaccacgt gaaagatata aatcgatgac atattcatat
     1441 aacgctaaat tgtcatacaa gtaagcgctc ttagcattta attcgaatgc caatttattt
     1501 ttgattactt gatc
//
LOCUS       DACEOM010000174         1507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEOM010000174 DACEOM010000000
VERSION     DACEOM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.182"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1361
                     /locus_tag="IW781_003020"
     rRNA            <1..1361
                     /locus_tag="IW781_003020"
                     /product="23S ribosomal RNA"
BASE COUNT          425 a          322 c          445 g          315 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg acttaaccaa taggttaaaa gaaaagtact
     1381 cggaaaaaaa cttatgtgac ttcaaatcca gttttgagtg aacaaaattt actcaataaa
     1441 agataacacc acagtgtggt ggcgatagcg agaaggatac acctgtaacc atgccgaaca
     1501 cagaagt
//
LOCUS       DACEOM010000175         1425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEOM010000175 DACEOM010000000
VERSION     DACEOM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.183"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            92..1387
                     /locus_tag="IW781_003021"
     CDS             92..1387
                     /locus_tag="IW781_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1183972.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          450 a          282 c          267 g          426 t
ORIGIN      
        1 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
       61 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      121 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      181 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      241 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      301 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      361 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      421 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      481 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      541 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      601 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      661 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
      721 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
      781 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
      841 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
      901 taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct attggaaact
      961 cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa
     1021 agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc ccaacttacg
     1081 ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag atcagtcact
     1141 atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata caaccacttt
     1201 acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca gctattcaaa
     1261 cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg cgtacggctt
     1321 tcgcaacttt tacaatttca aactacgaat tatgattcgt catggcaaaa catttttaac
     1381 caaataaaca gaacagaaag aggttttctt ctttctgttc tgtcc
//
LOCUS       DACEOM010000176         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEOM010000176 DACEOM010000000
VERSION     DACEOM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.184"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW781_003022"
     CDS             complement(150..1337)
                     /locus_tag="IW781_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1183973.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEOM010000177         1420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEOM010000177 DACEOM010000000
VERSION     DACEOM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.185"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..742
                     /locus_tag="IW781_003023"
     CDS             200..742
                     /locus_tag="IW781_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183974.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="IW781_003024"
     CDS             898..1347
                     /locus_tag="IW781_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1183975.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
BASE COUNT          559 a          181 c          274 g          406 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa
//
LOCUS       DACEOM010000178         1336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEOM010000178 DACEOM010000000
VERSION     DACEOM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.186"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(146..1129)
                     /locus_tag="IW781_003025"
     CDS             complement(146..1129)
                     /locus_tag="IW781_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1183976.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          377 a          285 c          254 g          420 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     1321 catttgcggt aataag
//
LOCUS       DACEOM010000179         1309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEOM010000179 DACEOM010000000
VERSION     DACEOM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.187"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..>1309
                     /locus_tag="IW781_003026"
     CDS             128..>1309
                     /locus_tag="IW781_003026"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1183977.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
BASE COUNT          410 a          262 c          235 g          402 t
ORIGIN      
        1 ctctataatt tctaggactg atggaaattt tctatctgtc ctagtttttt tgtaaaaaaa
       61 caaaaagaca tacaaaattt ccggtagaat agagtctcct acaacatcat tctgggggaa
      121 ttcttgtatg tccatgaatc attttatcaa acaactcttt actattaaag actcaaatat
      181 ccaaattcaa tcctttgaag aaacttttta taaaagcaga aaaagtatcg ttattgaggg
      241 aactctgatc cgtacctaca gacgttgtcc gtgttgtaaa gattctacaa agcaaatcgt
      301 taaaaatggc aagaagatct ctatgattct cctgaatcgt agcggtaata aacggaccta
      361 tcttcggttg aagaaacagc gatacctctg ccgagcatgt aaaaaatatt ttaccgctcg
      421 gacgtatctc gttaccccat tctgctttat ttctaagcag attcattaca agatcttaga
      481 agaattgaca gagcgtcaat ctataaaagc aattggtaaa cattgcgatg tttctgtgac
      541 aactgttcag cggacattgt ccgccttgtc atcagatatc cgtcagaatc ttgagtattt
      601 acctcggtgt ctattattcg atgagtttcg ttctctttct acatggcacg gtaaattttc
      661 tttttcttgt atggacggtg agaccgggaa actgtttgag atcttaccta gccgccgtaa
      721 aaaagatctc gttgcttttt tcatgcgttt ttctctcaaa gcgaggttgg gtgtacatta
      781 catcgtaacg gatatgaacg ctccttattt ctcactagtt aaagagtgtt ttccaaatgc
      841 acaagtgatc gtcgatcggt ttcacattgt tcaacatctg aaccgcttct tcgatgcggt
      901 tcgaaaacgt gtgatgaaaa cacttgatca aaaagaccca atacaagcta aacagtatcg
      961 ccaattgaaa tccttattca aattactttt aaaatcagag gatcgtttag attacaatac
     1021 actaacgaaa agacgcaatt tcaattggag actattgact gaaacagaag tagttgaccg
     1081 ccttctaaag atttctcctg aacttaaaac agcttatcgt tactaccaag acctgcttac
     1141 tgcgtatcag gaaaaagatc cagatacttt tttccagctg cttcgagcaa tgcctggaga
     1201 agttccattg gaactgcacc atatcaaaaa agcctttttc aaatacgaga aagggattcg
     1261 gctggcatta ctgaagaagt attcgaatgc acgactggaa aatctccat
//
LOCUS       DACEOM010000180         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEOM010000180 DACEOM010000000
VERSION     DACEOM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.188"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1244
                     /locus_tag="IW781_003027"
     CDS             <1..1244
                     /locus_tag="IW781_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1183978.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACEOM010000181         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEOM010000181 DACEOM010000000
VERSION     DACEOM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.189"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            414..947
                     /locus_tag="IW781_003028"
     CDS             414..947
                     /locus_tag="IW781_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1183979.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACEOM010000182         1202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEOM010000182 DACEOM010000000
VERSION     DACEOM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.190"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(36..>1202)
                     /locus_tag="IW781_003029"
                     /note="aph(2'')-Ia"
     CDS             complement(36..>1202)
                     /locus_tag="IW781_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ1183980.1"
                     /translation="DEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVIL
                     DPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLI
                     EHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNL
                     ETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQM
                     HGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGK
                     KCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDI
                     LRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          395 a          206 c           89 g          512 t
ORIGIN      
        1 atgtctttta taatagcttt tcatatatta tataatcaat ctttataagt ccttttataa
       61 atttcttttc taccattttc gataaattcc tgtttaatat ttttaattcc ataaacaata
      121 gtttcaatag gataatattc ttcaactata tcttgatatt cttttgcttt ctcaatatct
      181 atatttccat acattcttaa tatatcttct ccaaaatttg ttcctatttc ttcttcacta
      241 tcttcaagta agtatataaa atcacaatat tcatctataa ttccagaatc tccaaaatca
      301 attattccag ttaatctatt attgccatct aacaatagat gattacaact aaaatcatta
      361 tggcataaac actttttacc ctcaaaaact gttgttgcat ttagtctttc cataaaactt
      421 tctatataat ctttttctat atcagttaaa tcattataaa tagtttcacg caacaatata
      481 tactcttcta atacattttg tttattatca atagtacatt cactaatatc tgtataatct
      541 aaaccgtgca tttgtcttaa aaaactggca atatctcgtt ttaacaaatt ttgttcttct
      601 tctgacatag tagaataaat ttctggtgtt aaaaaagttc ctttaatttc tttataacct
      661 agtatagata attcatcact aatatacgaa tattcaatat taggaatttt tacattagtt
      721 tctaaatttg tatttaaaaa attatatatt gctttttctt ttgcataacc ttttttctta
      781 ttagtactaa attttgtttt aaaaatgtat tcattattaa ctaaatatgc cacactatca
      841 taaccactac cgattatttc aatactatct actttgaaat tatcaaagta atgctcaatt
      901 aaatatttca ttgccttaac atttgtggca ttatcatcat atctatattc cattaaataa
      961 caatcttctt ttttgccctc gtgtaattca tgttctggca aatcttcaat aattctaaaa
     1021 ccagattttt ggtatgccct tattgctctt ggattatttt tatgagggtc taaaataact
     1081 gcattagcat ttctttcttt tttcaaaaat tcaaaaatca atttaatata tcttgtacca
     1141 attcctttac tccaataatt tggctctcct ataaattgat ccataccata gactatctca
     1201 tc
//
LOCUS       DACEOM010000183         1198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEOM010000183 DACEOM010000000
VERSION     DACEOM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.191"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            67..939
                     /locus_tag="IW781_003030"
     CDS             67..939
                     /locus_tag="IW781_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1183981.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          349 a          224 c          283 g          342 t
ORIGIN      
        1 taatttaaat atgatatata gtcatttatt tagtgatttc attctaaaat aaagggagaa
       61 atgtctatgc tatacggtgc aattgaagca ggcggcacaa aatttgtgtg tgccattggc
      121 gatgaagaaa tgacgatcaa ggaaagagtg agctttccga caacgacacc tgaggaaaca
      181 atgcctttag tcattgactt cttcaaacaa taccaagcag atttagcagg gattgggatt
      241 ggctcatttg ggcctatcga tatccatcgc gattccgcca cttatgggta cattacctcg
      301 accccgaaat tggcatggca aaattttgat ttcatcggta ccatgaaaaa agaatttccg
      361 atcccgattt cttggacaac agatgtcaat gcggctgcct atggtgagta tgtcttcggc
      421 agtggcaaag gattgagcag tgtcgtttac tatacgatcg gtacgggtgt tggcggcggt
      481 gccttacaag aagggcgctt tatcgaaggg tttagtcatc cagaaatggg gcatatgctc
      541 gtcgtccctc atccaaaaga tgactttgct ggcagctgtc catttcatgg caactgttta
      601 gaagggatgg ccgctggacc cgccatcgaa aaacgcttag ggaaaaaagg acaagaggtt
      661 gcagaagatg atccttattg ggaaatcgaa gcctcctata ttgctcaatg tgcctacaat
      721 acaaccttga tgttttctcc agatgtgatc atttttggcg gtggtgtgat gaaacaacgc
      781 catatgctgc aaaacgtcca cgacgcattt gcaaaattgg tcaatgggta tgtcgaaaca
      841 cctgagttaa gcaaatatat cgtgacccca gcattagaag acaacgctgg aacgctcggt
      901 tgtttggcat tggctcgaga agcggtctta cattcataaa cgaaaaaagg ctcttcgaca
      961 tttagtgttg gtagagaaag atacaactga aaatttagtc ataaccagtt aggcagagtg
     1021 cttaactggt ttttgtttta cttggaagat caagccttga atgagataaa ttcttgctcg
     1081 aaagttatgg aaatttcgat agccgtaggc cacttgtatt gtgcaatagt tttttacaaa
     1141 aaaaaggggg tgcggatatt tttttggacc tttttaacaa atatatcttt gcttcttc
//
LOCUS       DACEOM010000184         1196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEOM010000184 DACEOM010000000
VERSION     DACEOM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.192"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..>1196
                     /locus_tag="IW781_003031"
     CDS             82..>1196
                     /locus_tag="IW781_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1183982.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKL"
BASE COUNT          396 a          231 c          232 g          337 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttca
//
LOCUS       DACEOM010000185         1167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEOM010000185 DACEOM010000000
VERSION     DACEOM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.193"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..605
                     /locus_tag="IW781_003032"
     CDS             51..605
                     /locus_tag="IW781_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1183983.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            859..>1167
                     /locus_tag="IW781_003033"
     CDS             859..>1167
                     /locus_tag="IW781_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183984.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          478 a          118 c          213 g          358 t
ORIGIN      
        1 aatatatata taatcatgaa ctcagaatat ttttaagaag ggaaacaata gtgagctata
       61 atgtacaacc gttaaggaca aatgaagaga tacaagattt tcagttttgg ctaagaagaa
      121 caagtaatcc agaaagagat agttttttat ttttgtttgg aatcaataat gggttacgta
      181 tgtcagatat tataggatta aaagttggag acatacgagg taaaagtaag cctattattg
      241 ttgaacataa aacaggtaag cgaaaaccga tttttataga taatttgaga gaagagattt
      301 tattatatac tgaaggtaaa gaagaaaatg attggttatt tccaagtcgg caacaaggtc
      361 gacatataac aagggatcgt gtctatcaga tatatgcaga tatagcagaa aaattaggaa
      421 gagatgatat aggaacacat acattaagaa aaacttttgg gtatcactac tacaagaaaa
      481 ctagagacat tgctacctta atgtttattt ttaatcatag ctcgcaagca attacaaaaa
      541 ggtacattgg tataactgaa gatgaaatag gggcatcgtt acgtggtttt aaattgggag
      601 gctaattgag atattaggat atattttttg tgtataatga aaacttagaa ggtgagatta
      661 atgaagataa ctgaattttc ggaagtattt aaaacgttta gtaacattta caatagaata
      721 ccagagatag aacaaaatgt tgtagttgat aatgattatt atatagagaa tgattttaat
      781 aaaccaagaa aatatgacaa cctatacaat tttatacaca atagaacaac aataaataac
      841 aacagtaaca ctcgagtttt gattaattca gcatattttc atattaaata tttaaagaaa
      901 tatgagtatg aagaagttgt attagattat gggctaaata gacagtcatt tttaatttat
      961 gcaataaata acggaaattt gccaagcaat aaaaagaaca taattattga aacatatgcc
     1021 tatctggagg ggggatctaa aaaactaatt aagtcatgta atatagagta tcaattatca
     1081 gtaggagaaa ttaaatgtgt tttcgaagaa gatttagaac ttgacttaaa aaaaattttt
     1141 gccagcattc atgattatat aggtttg
//
LOCUS       DACEOM010000186         1157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEOM010000186 DACEOM010000000
VERSION     DACEOM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.194"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(118..987)
                     /locus_tag="IW781_003034"
     CDS             complement(118..987)
                     /locus_tag="IW781_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1183985.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
BASE COUNT          326 a          240 c          176 g          415 t
ORIGIN      
        1 cgaaaaaccg cgcagtttcc cccggtatac tattttctct ttctatctct agaataagcg
       61 atagtatgac cgttcataaa atgacgagac atgcgacaat cgttaagaaa gaaaaagtta
      121 agcgtcgcat cgaatgtcag ttttattctt ttcttttggc tcactgacga accaccattc
      181 gattcctata gcatcataga ttgtgagtac tgtgtgcttt ttgtctaaga agaaatcaac
      241 tgatagttgc tgcaagtgag caatcagtcc ttccatgctt tcagtatcga tcatgaattt
      301 cagaaaatca agtccaatca cttcatctgt aggcaaatca attgcgcctc cgctagcttc
      361 tgtcaaacca acatggaaat ctccttcatt caatacatgg atgccctctt cttcattatg
      421 taccttcaaa ccaagtacat gattaagaaa ttggcgttct tttgttaagt tagtaacatt
      481 taaatggatt ttttcaaaat aagaacctgt tgatagttta ctgaattttt cttcagattt
      541 tcttaataat gcttcttgat ccattgtttc tggaatttct ctttggtcgt tcttattctc
      601 ttcatggtaa atttccaatt cattctcttc tgggtctacg agcaaaatcc ccatacgtcc
      661 atgatcttct aaagcttctt tgattggata attcatttta cggatacgac aaaggatatc
      721 tgccatttct tctgtgctag ggataatcaa tgaaaaacgg ttcagctttt tgatcacccc
      781 catgtgatca ttcgctctag gactttcttc cagcagcaac atttcacttc cagcttcttc
      841 tgttcctaaa atggctaatt cgttttcttc tcttttcaaa tcaaagccaa caacgttctt
      901 gtaaaaatca atcattttat ctcggtcttt cacccgaatg gctacacttt ttaaataggt
      961 ggcagccccc aattgaaaat ctttcatatt tcttgctccc ctctacgtca agctttattt
     1021 taacgatgga ttcttcttct ttcaagcaat aactttgatt ttgttaaaaa aaatgtgaga
     1081 aaattcgcat tttgcttgtt ttttgtaaat taatgagtaa aaactcttat aaaaagaggt
     1141 tgtgaaaaag ctgttct
//
LOCUS       DACEOM010000187         1128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEOM010000187 DACEOM010000000
VERSION     DACEOM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.195"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            8..454
                     /locus_tag="IW781_003035"
     CDS             8..454
                     /locus_tag="IW781_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183986.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRTDGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLNNVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            456..593
                     /locus_tag="IW781_003036"
     CDS             456..593
                     /locus_tag="IW781_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1183987.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            631..924
                     /locus_tag="IW781_003037"
     CDS             631..924
                     /locus_tag="IW781_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183988.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIRPIVASFTKITKTLSQVSY
                     NLELLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            921..>1128
                     /locus_tag="IW781_003038"
     CDS             921..>1128
                     /locus_tag="IW781_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1183989.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESE"
BASE COUNT          401 a          172 c          241 g          314 t
ORIGIN      
        1 gaaataaatg aaaaacattt ggaaatatgg acgaacaggt ggagagtatg ctggacaagt
       61 gcttgatgat atggttatga ctgttccatt taccgatgtt ccaccactcg aaggaattcg
      121 tactgatggc gaaccgctaa cgattgctga tcagatgttt gatcctaaac tgaaccaatg
      181 gattgtttta gtgaatgtac tagatcacaa cgatttaaac aatctcaaag cgatgtatga
      241 gtcgttagaa aatgagaacg gcgatttaaa acagctcaac gccaaactca tgctaaacaa
      301 tgtagcaatt aaacaggaaa atactgcatt gaaagaaaaa gcggatagtt tagcacaaat
      361 caattcaaaa atgatgcttg cttcgttaca aaatagcaaa gacatttcag aaattaaaga
      421 gcaactaaat ccagcttcaa agggaggtga gtagtatgtt tagttttagc gatgtgaaaa
      481 tgatgtatga ttggggctgt tttactgacg atcaagttcg actattcgtt ccactatgca
      541 ttacagacga agaagcagat aaaatcatta gcaaagaaga gagcgcatct taattgatgt
      601 gctttttatt ttgattcaag gagttgtcac atgattaatt taggggaatg gggagcgata
      661 gcaggatcaa taaccgctat cgtttctttg attttattag taattagacc gattgttgca
      721 tcatttacaa aaattacgaa aacactatca caagtgagtt acaacttgga actattaaca
      781 aaagatttag aatcgagcaa atcagatcga ttgatgattc atgaagaact aaagaaacac
      841 gatgaaagat tagatacaca tgcagaaaaa ttggtagaac acacgcaaca aattaaaact
      901 ttatttaggg agagaagaaa atgaataata aaacgttcga agtactaaaa tggttcgcac
      961 tggtaattat tcccgcacta gctactttcg tggggttagt tggtaaagcg ctcaattggc
     1021 agtacacaga tatctgtgtt gtcatcatta ctggttttgg cgcgttttta gggagtgtgt
     1081 tgggtgtatc aaatcgaacc tacaaaatgt tctcggctga aagcgaag
//
LOCUS       DACEOM010000188         1109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEOM010000188 DACEOM010000000
VERSION     DACEOM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.196"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..>1109
                     /locus_tag="IW781_003039"
     CDS             50..>1109
                     /locus_tag="IW781_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1183990.1"
                     /translation="MANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQ
                     IKDNINVNLESDSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLL
                     ISILFSAIISLIYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDL
                     YSTLLRTIDDLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYK
                     ERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQ
                     KKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYG
                     NNKISNMDKIFDVKFDLNK"
BASE COUNT          506 a           87 c          153 g          363 t
ORIGIN      
        1 tatctttcct aaaacatatt tggagacgag aaaagaaaag attcataata tggcaaatga
       61 aatttctagt aatatgaatg gaaaagaaat aaaatatata gaacaaactt tggagtttta
      121 ttccaagagt agtgaaataa aagtattcat aaaagaaaaa aataataaga atgaaataca
      181 aatcaaagat aatataaatg ttaatttaga aagtgatagt aattctttga taattgaaga
      241 aagagaaata aagcttaatg atggtaaaaa aacaaatcta caatttgttt ctacagctga
      301 tatgcaaaaa gatgcgaaag atttaagcct taaattttta ccatattctt tattaatatc
      361 aattttattt tctgctatta tttctttaat ttatgcaaaa tcaataaaaa ataatataca
      421 agaaataaaa attgttactg ataaaatgat gaaacttgat aaaaaaatga gtttaaaagt
      481 tagctctaat gatgaagttg gagaattaaa acaacaaatt aatgatttgt attctacttt
      541 attaagaaca attgacgatt tggaatttaa aaataaagaa attttaaaat tagaaaagtt
      601 aaaatatgac ttttttaaag gtgcatccca tgaacttaaa acccctcttg ctagtcttaa
      661 aataatactg gaaaacatga aatacaatat tggaaaatat aaagaaagag atatttatat
      721 taatgaatgt attgaaatcg ttgatagttt aacaaaaaat atttctcaaa tattatcagt
      781 tcattctata gaaaatttga ataatgatga agaatatttg aaaataaatg atacattaga
      841 agacatatta aaaaagtatg aaatattagc aaatcaaaag aaaataactg tcaataatta
      901 tatattagac gagaatgttt atataggaaa aacagcttta aagattattc tatctaattt
      961 gattagtaat gcggtaaaat atactgatgt aaatggggta attaatattg ggatagttaa
     1021 tgattggtta tatatagaga attcatacgg taacaataaa atttcgaata tggataaaat
     1081 atttgatgtt aaatttgatt taaataagg
//
LOCUS       DACEOM010000189         1093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEOM010000189 DACEOM010000000
VERSION     DACEOM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.197"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            524..>1093
                     /locus_tag="IW781_003040"
     CDS             524..>1093
                     /locus_tag="IW781_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ1183991.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          369 a          169 c          191 g          364 t
ORIGIN      
        1 agataaattc agttcattaa ccgtttgtcg aacacttacc cgattagaat tttaaatacg
       61 tttttgacta cgttttgaaa tttagttagt actaatattt tataactaga agcaaaaatt
      121 agggtaaatc gctaaaatat gctagtaatt ttcttctatt accagtcata tataaatcct
      181 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
      241 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
      301 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
      361 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
      421 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
      481 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
      541 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
      601 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
      661 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
      721 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
      781 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
      841 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
      901 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
      961 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     1021 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     1081 gctatcaaat gga
//
LOCUS       DACEOM010000190         1063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEOM010000190 DACEOM010000000
VERSION     DACEOM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.198"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(38..994)
                     /locus_tag="IW781_003041"
     CDS             complement(38..994)
                     /locus_tag="IW781_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183992.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DACEOM010000191         1048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEOM010000191 DACEOM010000000
VERSION     DACEOM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.199"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..980
                     /locus_tag="IW781_003042"
     CDS             6..980
                     /locus_tag="IW781_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1183993.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          357 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatt
//
LOCUS       DACEOM010000192         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEOM010000192 DACEOM010000000
VERSION     DACEOM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.200"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>1035)
                     /locus_tag="IW781_003043"
     rRNA            complement(<1..>1035)
                     /locus_tag="IW781_003043"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACEOM010000193         1025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEOM010000193 DACEOM010000000
VERSION     DACEOM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.201"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          378 a          129 c          155 g          363 t
ORIGIN      
        1 tatttatatt aagtactacc gttctaggaa taagataata aaaaaaaact accaaattgc
       61 aattaaatac tacttaaaat gtaaaaaata agaactaccc aaattaggta gttcttaata
      121 agagaatatc agataaattt gtacaagtca taaaaaaatt aatcttaaat ttttaaatat
      181 atatacctgt taattataaa ctactttatt taggtagtac ataattaaca gataaaacta
      241 catgcttgta gtttgtctag ctaatgagtt gcatatgatt tatataaagg tgtgcagcat
      301 aacttagttg aagttaagag aatagaataa atattttttg ttttgctaat aattaaacac
      361 tacctaaata tggtagtgtt taattatata atattcattt tgttacaaat aatcgtatta
      421 taaaagcata acaattggga taactatttt ttagattatt taagtactac ttaaattagg
      481 tagtacttaa attttggttt aaggtagtag gtaattaaga aataatatat atcttctttt
      541 ataatagaag gggagaggcc tagttaaaaa cagcgacaat taataacaat gtatttaatg
      601 cttcacaaaa tccatttggt ggacaatgca ttgttattag cgatgatcta ccgttctaag
      661 tagttttttc tattggttgc attcgtgact aattatggta taattatgag taacaaagag
      721 gtatttcatt atatcatcaa accccatcag cgtgtaggct gatggggttt tttttacgtt
      781 tctatattat agaattaact ggtaggctta ctgtcttttt tggcaatttt gcaatcaagc
      841 cacttgcaga tgtagtgtgc agttacctct gcaaatggct tgattgcaaa aacaaaatca
      901 aaaaggtatt tcattatatc tcctcctccc cttgccagat taggccgggg tagcagacag
      961 caaaactatt ataacataac tgaaatacct aaatgacata tacagatatg ataatttatg
     1021 tattg
//
LOCUS       DACEOM010000194          989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEOM010000194 DACEOM010000000
VERSION     DACEOM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.204"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..957
                     /locus_tag="IW781_003044"
     CDS             <1..957
                     /locus_tag="IW781_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1183994.1"
                     /translation="NAFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNEA
                     QYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKVTF
                     EKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMG
                     VSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENA
                     NLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSFN
                     MRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          347 a          183 c          200 g          259 t
ORIGIN      
        1 aacgcctttg cttacacgat caacaatgaa ttcaatttgg gcgcaaacga aggtagttct
       61 caagtagcaa ataaccaata catcctattg catgaaacag caaacgaaac tgcgacagga
      121 cgaaatgaag cgcagtacat gaaacgttct tggtacaatg cctatacagc gtatattgtt
      181 ggcgatggtg ggatcgttta ccaagttgga caacctggtt atgtacaata tggcgctggt
      241 tcatatgcta atgcaaatag tcctgtgcaa atcgaactgc aacatacgca cgataaagta
      301 acgtttgaaa agaactacaa ggcatacgtt gaattggcta gagattcagc aatgaaatat
      361 ggtattccat taacattaga cacgccttat aaccaaccag gaatcaaatc acatttatgg
      421 gtaacacaaa atatctgggg agatcatact gatccatacg gttatctttc tgaaatgggc
      481 gtaagtaaag aaaaattagc atatgattta gctcatggat ttaccgatga aaatccaaca
      541 acttctgaaa acaagcctgt cattgatcca acacgagctg gtgcagctaa tcctacactg
      601 acagatggaa cgaattacgc ccacattgat cagtttggag aaatcgaaaa tgcaaatttg
      661 catgtagctg gatggcacat tgctaactat aaatacgagt atatcttcat tatggattac
      721 aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagatc agatgtaaat
      781 caagcttata atacttctgg aaatgttggc tatcatgtat cttttaacat gcgtaacttc
      841 cctaataaga aagtctatgt catgatgcgg gcaacgaatg atccagaggg aaacactaaa
      901 ggcggtgcgc aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa
      961 tagctcctcg ttgaggagca ttacataac
//
LOCUS       DACEOM010000195          986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEOM010000195 DACEOM010000000
VERSION     DACEOM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.205"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..986
                     /locus_tag="IW781_003045"
                     /pseudo
     CDS             1..986
                     /locus_tag="IW781_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
BASE COUNT          425 a          129 c          164 g          268 t
ORIGIN      
        1 cggtgcttct tcacttgaga gccaaaaaag gggcagaaaa attagtatga attccaagct
       61 aaacattcct aaaaaactta aagatgagtc tcttgaagaa gaagttattc gtttaagagc
      121 agagaacgca tatttaaaaa agttagaaac cttgattcaa gaacaagatt tgagcaagaa
      181 gaaatcaagg ttaaaacaat ccttcagctc aaaaaagaat ttaaattgaa tctattactt
      241 tcaattgcac aattagcgaa gtcaacttat tattactggg taaaaaaatt agataagcca
      301 gataaatata gcaagataaa gcaagaaatt acagcgattg taaaagaatc aagaaactct
      361 tatggttatc gtagagtcac tttagcgtta aagatgaaag gatatacaat caatcataaa
      421 acagttagaa aattaatgtc ccaaatggga cttacctgtc aaatccgaat aaagcgatat
      481 aaatcttata aaggaacagt aggaaaaatt gccaagaatg tgttaaaacg aaatttttca
      541 gtagatacac ccaataaaaa atgggtgaca gacgtcactg aattcaagat aaaaggaaga
      601 aaaatctatc tatctcctat acttgattta tttaacggag aaataattag ttatagtata
      661 tcaactagtc caacatataa actgattgaa gaaatgcttc aacaagcaat taaaaaaaag
      721 ggaactgagg gttcactgat tctacattca gatcaaggat ggcagtatca gatgccacaa
      781 tatcaaaaaa aattaaaaga gaataacatt attcaaagca tgtctagaaa aggaaattgc
      841 ttagataatt ctgtaataga aaattttttt ggtgtgctta aatcagagtt tttttatcga
      901 gaaaaattcc gatcaattga gatatttcaa agtaaattaa atgagtatat taggtggtat
      961 aataacaaaa ggataaaact aaagtt
//
LOCUS       DACEOM010000196          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEOM010000196 DACEOM010000000
VERSION     DACEOM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.206"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>943
                     /locus_tag="IW781_003046"
     rRNA            2..>943
                     /locus_tag="IW781_003046"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACEOM010000197          909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEOM010000197 DACEOM010000000
VERSION     DACEOM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.207"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>909
                     /locus_tag="IW781_003047"
     CDS             <1..>909
                     /locus_tag="IW781_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1183995.1"
                     /translation="KDAKVVSGEQRINREDLSDEFKNVVSLEATNNTKRNILFSSGVF
                     TIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIF
                     SGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALN
                     KLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWL
                     RERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKVIVDGFINSE
                     DSMISGGSLINNSSFMSNITTLGQSY"
BASE COUNT          370 a           80 c          154 g          305 t
ORIGIN      
        1 taaaagatgc taaagtagtt agcggagaac aaagaataaa tagagaagat ttatctgacg
       61 aatttaagaa tgttgtttca ctcgaagcta caaataatac taaaagaaat attttattta
      121 gtagtggagt atttacaatt aaagaaggta aaaatataga agaaaatgat aagaattcaa
      181 ttattgttca tgaagaattt gctaaacaaa acaatctaaa attaggtgat gaagttgatc
      241 ttgaattact agatattgaa aaaagtggaa aaataaaaag tcataaattt aaaattatag
      301 ggatcttttc tggtaaaaaa caggaaacat atacaggatt atcttctgat tttagtgaaa
      361 atatggtgtt tgtagattat tcaacaagcc aagagatatt aaataattca gaaaataatg
      421 aaattgcaaa taaaatttta atgtattctg gtagtgcaga atctacagac ttagccttaa
      481 acaaattgaa agagcttaaa attgatgagt caaagtattt tgttgaaaaa gattctaatg
      541 cgtttgaaga gtctttagag tcagtgagtg gaataaaata tataataaaa gtaatgactt
      601 attctattat gttaggtggg atggttgttc tttcattaat cttgattcta tggttaaggg
      661 aaagaattta tgaaataggt atattcttat ctattggaac atctaagata caaattataa
      721 tgcaatttat attcgagtta atattcatat cgataccaag tataatatct tccttatttt
      781 taggaaatgt attactaaaa gtaattgtag atggattcat taactcagag gattcaatga
      841 tttccggtgg aagtttaata aataatagta gttttatgtc aaatataaca acacttggac
      901 aaagttatt
//
LOCUS       DACEOM010000198          887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEOM010000198 DACEOM010000000
VERSION     DACEOM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.208"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..506)
                     /locus_tag="IW781_003048"
     CDS             complement(<1..506)
                     /locus_tag="IW781_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1183996.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYK"
BASE COUNT          295 a          154 c          167 g          271 t
ORIGIN      
        1 catttgtacg ttagattttc aagaaaatgt tgctttgtac gaatcaattg cttaataagt
       61 tgatagcgag tacgtgttaa acgctgtaag gccatgtatt tctcttcttt gataggggaa
      121 gttgtgaatc gttgaatacg caagaaatca gcgattctta atgcatcgtt tcgatcagtt
      181 ttattctcat caaacatgcg actaaattgt ttcacccgaa aaggattttc aatcgttaca
      241 agtgtgttga gcttctttag tttctcatct tcatgaaaaa acatggaagg atggaagctg
      301 tacatggacg ttgactccat gccaatcaca atttgatcaa agtggtagct gttattgaat
      361 tcaagaattg tttctcgtat gaatgaagca ccttcgatgt cattggcaac agagatttca
      421 gataagatag acaattgatt gtcgtctgtt aaaaaacaaa catctagttt ttcagagcta
      481 acgtcaatac cgacaaataa tttcatggaa gaaggacctc ctttcgatat aaattttgga
      541 aaatgcttcg atactgtggg atttcccaga aactcactgt atagccacaa cctcgcctaa
      601 tagaatacgg tgataaaaga gctagaagct gccttccaaa ctactatttg aaagtctagt
      661 ccattatcga aacagctaat gagttggtag tgataacagt ggttcgggta acagactaaa
      721 cagggtagac aaacgctaca ggaggaaata gcaaattccc gttggatcct ttccatacat
      781 ccattgtact ctgagaaatc acacaatatc gaaacaaatt ccgttaggaa tgtgttattc
      841 aataaatttt ataatctaga aacattttga ataaaaacta ttagaca
//
LOCUS       DACEOM010000199          887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEOM010000199 DACEOM010000000
VERSION     DACEOM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.209"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..887
                     /locus_tag="IW781_003049"
     CDS             <1..887
                     /locus_tag="IW781_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1183997.1"
                     /translation="VPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKI
                     IKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEE
                     KVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELE
                     VIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEF
                     DVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTF
                     TKLAKAEQYRRSLELSKD"
BASE COUNT          389 a           96 c          148 g          254 t
ORIGIN      
        1 ctgtccctct tattttaaat aaacgagaga aaaataattt aaagaaaaaa atgctcattc
       61 catttgattc agaagtgaaa ggattactca ataaactaac tatttctaat atagactcga
      121 ctagggaaaa aatcataaaa atatttgata aaattaataa tttattcaag aatgacaatg
      181 ctgaattaag gaagggtctt ttagagttta aacttcaaga actagaactt cattactctt
      241 atttagtgaa aattaccgaa gaaaaagaac aacaaaaagc aataaaagaa cagatgatag
      301 aagaggaaaa agttcgtaga gaaattgatc gtgaaaagaa acgtattgat aaagaacaac
      361 gtcaattcaa ttctgaaatt tccaaattaa tcgcatatat gcaaaaagct aatgcagatg
      421 tggaaaaaga attatatgca aataaaattc aagaactgga agaaaaatta aaagaattag
      481 aagttatacg tgaaaatgta ttacaaagag aattaaatac tagagccggt tatgtgtatg
      541 taattagtaa tattgggtct tttggtgaag acatatataa aattggtatg actagaagat
      601 tagaacctat ggatagaata aaagaattga gtagtgcatc ggttccattt gaatttgacg
      661 ttcatgcgat gattttttca gaggacgctc cgtctttgga aactaaattg cataatcatt
      721 ttagaaaaca agaagttaat aaaattaatc aaagaaaaga atttttcaaa gtttcactag
      781 acgaaataga gaaagttgtt ttagaaaatt ataatggaac agtaacattt acaaaattag
      841 ctaaagctga acaatataga cgatctttag aattgtcaaa agattaa
//
LOCUS       DACEOM010000200          876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEOM010000200 DACEOM010000000
VERSION     DACEOM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.210"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(555..869)
                     /locus_tag="IW781_003050"
     CDS             complement(555..869)
                     /locus_tag="IW781_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1183998.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          333 a          127 c          129 g          287 t
ORIGIN      
        1 tggttgttgc cacataaagc cataaacaat caaccagtag aatgcgtggc aacaaacctg
       61 ttatcgcata tcttggagtg tgactattta tgagtgatag tgaagatatg cgtctccata
      121 tatgtatggt tgagacagat aaacatgtat gaaaaacgtt gttaaatcaa cgttttttta
      181 ttttggtcaa ggagagaaaa gtagagaaat acaaaatttt ttgcattgat tttgcattca
      241 aaaaaacatt caaacattta gaaatcccca atacgtattc aggaaaatct attttaaggt
      301 ttacaaaaag aacttttgtt cgtacactct ttttgaggtg aacttttatg ctgatggaag
      361 gaaaaacaca attatggttt aagcatgttc cttcaaataa gttttttaat tataaactct
      421 agagaaatta attcatccta acataaaata attgttcact caaaagtttt tctagaacaa
      481 ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt cctgtactaa
      541 aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag tagttgtgta
      601 ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga aacgtctata
      661 acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc tccgtaaaaa
      721 atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt tctaaaacct
      781 atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa tattgctatt
      841 attaagataa aattactgta aaaatccaaa agctat
//
LOCUS       DACEOM010000201          864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEOM010000201 DACEOM010000000
VERSION     DACEOM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.211"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            171..824
                     /locus_tag="IW781_003051"
     CDS             171..824
                     /locus_tag="IW781_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1183999.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          362 a           80 c          154 g          268 t
ORIGIN      
        1 caacacaaaa tattatagaa gccttttttc taaaggattt caaggtgtct gtttgcattt
       61 attaaaaaaa agaaaaagta ttggcaacag aaggcgtcac caacactaga tattatagag
      121 ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat atgaaaatat
      181 taactgttga agatgataat atgataagag agggaataag tgaatatctt tcagaatttg
      241 gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt aatagcgata
      301 taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa gtgttgaaag
      361 aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt gatgaagaat
      421 ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca ttttcattgc
      481 cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat ttagagaagt
      541 ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata aatggtgaag
      601 aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat aataatggac
      661 aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa gaaactcctt
      721 atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa ttagactgta
      781 taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat taaagatatt
      841 tccaaaaatg ttcatacaaa tatt
//
LOCUS       DACEOM010000202          795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEOM010000202 DACEOM010000000
VERSION     DACEOM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.212"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          307 a           89 c          151 g          248 t
ORIGIN      
        1 aagaacctca agtattgtct taaatcattg ttatgaaaat ggattaaaag agttcatcaa
       61 ggtgtgatta acaagttact tttcattaca tcgaattttt cagaagttgc ttatattggt
      121 gatgatatta atgatttatc atgtatgaca tctgtgaaga agcgggagga atagtagaat
      181 gtccaaaaaa tgccacaaga aatgagattg aattatctga ttttattgta actcacagtg
      241 gtggtgacga atctgtgata gagtttattg agtggatatt ggaatcgata tagtgtagta
      301 gtacagaaaa attatcatac aattaatact aggcataaga atattggcat attctagagt
      361 taaaagtaac tattcgaaaa ggataaggtt agaatttttt gttttgccag tggaagaata
      421 taaaaagggc attggaaatt aatatattag aatgctaagc ggagaaaaaa atatatgaaa
      481 agtacgtcct tgtgatggga tggaaaaata cacttatttc aaacaacgat aatataatta
      541 tgtgaaccga agtatactcg cttctaattc aatcctcaaa tcccgacagt cgcgacttca
      601 aaaaagcgta tagttttaat gtatccccat atgatccgaa aaatggtaat ttacaattaa
      661 cagtttgatg atgttgaaga tgtggaaatg ctgaaattac catgatgaga aacacattaa
      721 ttaaagggaa ttaattaact agaatataat ctgattcaat ttgaattaag caaaaaaagg
      781 ctgagtttta aataa
//
LOCUS       DACEOM010000203          786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEOM010000203 DACEOM010000000
VERSION     DACEOM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.213"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..717
                     /locus_tag="IW781_003052"
     CDS             1..717
                     /locus_tag="IW781_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1184000.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          281 a          151 c          159 g          195 t
ORIGIN      
        1 ttgaaaaaaa ggattttagt aacaattggt ctagcttttt tgttagctgg ttgtaccaac
       61 gggaatgaat cgaaaaaaga atcaacaaaa gaaacagcga aagcaacagt tgcaactact
      121 acaacaacta gcacagagag caagtcaact gcagcaagta cagaaacagc agatacctct
      181 acttcgtcag agtcttccaa aacgactgaa acaactcaaa catctacttc aagttcttct
      241 gtagaggctt catcttcctc ttcagaaagc agcgaacaag ttgaacaaac actgtggaat
      301 gcagacaaat ccgcacaatt agaggcattt atgaccaaat ggggccaaac aatgggacaa
      361 caatacaaaa gttatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
      421 gctattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
      481 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
      541 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
      601 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt caacgagaca
      661 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atggacaagc aaattaatga
      721 gataaacaag gggttgtgac atgtctttta tcacaacccc ttgctcagtt tatttgttag
      781 tctttt
//
LOCUS       DACEOM010000204          771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEOM010000204 DACEOM010000000
VERSION     DACEOM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.214"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          254 a          138 c          114 g          265 t
ORIGIN      
        1 cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt gacagataag
       61 atgagaaatg aaacggtcga tatcatggag aatctgagaa agaattcgta agttttgcca
      121 ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg gttatttttt
      181 actctgtttt caaacaatcc ttgctacttc ttactttctt cctcatccat tttcagaacg
      241 gccatgaagg cttcttttcg caataatcta actgtactat tctctattaa tctaagatag
      301 tctctaagct tctagtatta aaggttataa aggaactaaa taatctcttc tatctttagg
      361 tcatttcaaa acacctgaga aaacctcagt catcggtggc aaaatggtgg caatattttt
      421 ttgctgcatt attaaaaata gtaacaaaaa ctttatcacg atctttccat ttccatcatt
      481 atcttaccaa tttcattttc aattctatct agaactcttt gtatttcaga tctaacattg
      541 taaatatctt caatatactg ttgcgctgta taattatctg gtattttatt atcactagga
      601 ctaggatctt taatttctaa caaccctaat atttggtcgg gatacatatc aattacctta
      661 aatttcaaag aagatgcgta taaccaagat attttaatag aaggaaagac acaattatgg
      721 tttaaatttg atccttcgaa tagatttatc aaagactttt ataaagtatg g
//
LOCUS       DACEOM010000205          753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEOM010000205 DACEOM010000000
VERSION     DACEOM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.215"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>753
                     /locus_tag="IW781_003053"
     CDS             <1..>753
                     /locus_tag="IW781_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ1184001.1"
                     /translation="SLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPR
                     VLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLV
                     TDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKL
                     KKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHE
                     LVEAFRDKDPDLFFSLLAELPETLDDGFREKL"
BASE COUNT          239 a          148 c          145 g          221 t
ORIGIN      
        1 ttctttactg actgaaaaag tatctttatc ttttattgcg aaaaattgtc aggtatcttt
       61 gaccaccgtt attcgtacgt tgaaagagtt taaaagctac ttaccaaagc aatctaagag
      121 gattctcccc agagtattga tggttgatga atttcgttcg cacgcttcca tagaagataa
      181 aatgagcttt atttgcgcag atggcgaaac aggacaatta attgatgtct tgcctacgcg
      241 taaattgcct cgattaacaa gctatttctt aggctgcgct aatcctgaag aagtaaaatt
      301 tttagtcaca gacatgaacg ccgcctactt ccagcttacc aaacgtattc tgccaaatgc
      361 gaaagtagtg attgatcggt ttcatattat caaacacatg aatcaagcct ttaatgagtt
      421 gcgtatccgt gaaatgaatg aacttcgtaa agtaggacag aaaagtcagg cagaaaaact
      481 gaaaaagaac tggcgctttt tgctaaaaaa tcgtgcaaac atcaaccatt atgaatacaa
      541 aacatggaaa agttttcgag caccaaaata cccatttctt acggaagcga tgatgattga
      601 tcgattgctt ggcttttcta cgtccctgaa ggaggcgtat ccctattttc atgaattagt
      661 tgaagccttt cgagacaaag accctgactt atttttctcc ttattggcag aacttcccga
      721 aacgttagat gacggctttc gggaaaagct cca
//
LOCUS       DACEOM010000206          747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEOM010000206 DACEOM010000000
VERSION     DACEOM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.216"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>747
                     /locus_tag="IW781_003054"
     CDS             <1..>747
                     /locus_tag="IW781_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1184002.1"
                     /translation="NGFAYTINNEFNLGVNEGSSQVANNQYILLHETANETATGRNEA
                     QYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKATF
                     EKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMG
                     VSKEKLAYDLAHGFTDDNPTTSEDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENA
                     NLHVAGWHIANYKYEYIFIMDYNTGKELARV"
BASE COUNT          253 a          132 c          160 g          202 t
ORIGIN      
        1 aatgggtttg cctatacgat caacaatgaa tttaatttag gtgtaaatga aggtagctca
       61 caagtagcaa ataatcagta tattttactg catgaaacgg ctaatgaaac agcaacagga
      121 cgcaatgaag cgcaatatat gcaacgttca tggactagtg cttatactgc ttatattgtg
      181 ggagacggtg gaattgttta ccaagttgga caacctggtt atgtacagta cggtgctggt
      241 tcgtatgcta atgctaacag tcccgtgcag attgagttac aacacacaca tgataaagca
      301 acgtttgaga aaaactacaa ggcatacgtt gaattagcta gagattcagc aatgaaatat
      361 ggtattccat taacattgga cacgccttat aaccaaccag gaatcaaatc gcatttatgg
      421 gtaacacaaa atatttgggg cgatcataca gatccttacg gttatctttc tgaaatgggt
      481 gtaagtaaag aaaaactagc ctatgatttg gctcatggtt ttacggatga taatccaact
      541 acttcggagg ataaaccagt cattgatcca actcgagcag gtgctgcaaa tcctacgctg
      601 acagatggaa caaattacgc ccacattgat cagtttgggg aaattgaaaa cgcaaacttg
      661 catgtggctg gatggcacat tgctaactat aaatacgaat atattttcat tatggactac
      721 aatactggga aagaattagc tcgagta
//
LOCUS       DACEOM010000207          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEOM010000207 DACEOM010000000
VERSION     DACEOM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.217"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(626..742)
                     /locus_tag="IW781_003055"
                     /pseudo
     CDS             complement(626..742)
                     /locus_tag="IW781_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          221 a          160 c           91 g          270 t
ORIGIN      
        1 gttcttccat taataatcac aggttctgcc cctggaaact tttgtagata cataaaaata
       61 aaaaagtagc caagaaacac tcatagctta tgaatgtttt tgactcctat tagtactttg
      121 ctcaataccg atttatcagt agttataata tttttcttta tttcatatgc tgttaggctt
      181 ccgtcctttt ttcgccttac ttctgcatta tttcctcttc tgttatctcc aaaacagtaa
      241 gctatgaatt ttgcgcctgt aacatcgttt gcttatcctc tattggttca gccctacgta
      301 tatcgtacat tacctctttt cactttataa aattaaactc ttctactctc atccggtctt
      361 tccaagcaat aacctaacga atcatcactc aaacataaag agaagtgaac tgttgataag
      421 aatttgataa accattccga agtaccaaag caatactgtt tgtcacctag ctgttttcga
      481 actcctactt tactgaatct cacgcgaata tctgatttca tcacaccaat tagctctgta
      541 tttataccat acctaccgag catttgttcg ccaatcgttt ttcaaattct ctttttcaca
      601 aaacaagtta atactttctt aattgtcaaa ttaaattaga cagatcatca atacttacgt
      661 aactcaaaaa tacttccaaa ggtgttcgat aatcgttcga taatccagtg attttctagg
      721 aatattattt cgtttagatg cg
//
LOCUS       DACEOM010000208          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEOM010000208 DACEOM010000000
VERSION     DACEOM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.219"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW781_003056"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW781_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1184003.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IW781_003057"
     CDS             complement(481..675)
                     /locus_tag="IW781_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1184004.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DACEOM010000209          729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEOM010000209 DACEOM010000000
VERSION     DACEOM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.220"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..696
                     /locus_tag="IW781_003058"
     CDS             <1..696
                     /locus_tag="IW781_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1184005.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     RIMIRHGNALIFN"
BASE COUNT          243 a          150 c          128 g          208 t
ORIGIN      
        1 caactgcctt ttttaaaacg ttattttagt catttttcac gagaaattcg tgaagcggtc
       61 gaatggatcg tgattgatat gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca
      121 aaagcacagc tgattattga ccggtttcac atcgtccaac atattggtcg tacctttcgc
      181 aatcatcgaa tcaaagaaac gaaccaacta ttaaaaagca aagaacaaaa acactatcaa
      241 ttaggtaagc aattaaaaag atactggaaa cttcttcaaa aagatgaacg caagcttgat
      301 tatactcgac gcctttggcg acctggcttc aaagctcatt taacagagac cgatattgtg
      361 gatcgcttac tgaaagggag ccctgctttg cgtgtaggct atcagttata tcaagacttt
      421 ctttacgctg taaaggaacg agactacgta tcgtttgaag aactacttac caataatatc
      481 atgcttcccg aaggctatca gacgaccttg agaacgttcc agaaatttct gccacaaatc
      541 aaaaatgctt tacaacaaag ttattcaaat ggcccacttg aatgtctgaa caaccatatc
      601 aaagtgttaa aacgaaatgc atatggcttt cgtagctttt ataatttcaa attgcgcatc
      661 atgattcgtc atggcaacgc attgattttc aactaaaaaa gatcaagagg aactgatcca
      721 cttgatctc
//
LOCUS       DACEOM010000210          710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEOM010000210 DACEOM010000000
VERSION     DACEOM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.221"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..>710
                     /locus_tag="IW781_003059"
     CDS             94..>710
                     /locus_tag="IW781_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1184006.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKY"
BASE COUNT          241 a          129 c          147 g          193 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaatcc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga
//
LOCUS       DACEOM010000211          705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEOM010000211 DACEOM010000000
VERSION     DACEOM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.222"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..675
                     /locus_tag="IW781_003060"
     CDS             <1..675
                     /locus_tag="IW781_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ1184007.1"
                     /translation="HFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTT
                     IYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYL
                     NNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIPA"
BASE COUNT          242 a          123 c          139 g          201 t
ORIGIN      
        1 cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg ctactatctt
       61 cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt
      121 tctcatacga cgatttatcg ttgggtgcaa gaatatggca aactactcta tcaaatttgg
      181 aaaaagaaaa ataaaaaatc cttttattca tggaaaatgg atgaaacgta catcaaaatt
      241 aaaggaaaat ggcattattt gtatcgagcc atcgatgcag atggtttaac cttggatatt
      301 tggttacgta aaaaacggga cacacaagca gcctatgctt ttcttaagcg gttagtgaag
      361 cagtttgatg aaccgaaggt tgtagtcaca gataaagccc cctctattac aagtgccttt
      421 aagaaactaa aagaatacgg cttttatcaa gggacagaac atcgtaccat taaatacctg
      481 aataatttga ttgaacaaga ccatcgtcca gtaaagagac gcaataaatt ctatcgaagt
      541 ttacgcactg cctctaccac gattaaaggc atggaagcca ttcgaggatt atataagaaa
      601 acccgaaaag aaggcactct cttcgggttt tcggtctgta ctgaaatcaa ggtattattg
      661 ggaatcccag cttaaatcat agataccgta agggatttta ttctt
//
LOCUS       DACEOM010000212          664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEOM010000212 DACEOM010000000
VERSION     DACEOM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.223"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>664
                     /locus_tag="IW781_003061"
     CDS             <1..>664
                     /locus_tag="IW781_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1184008.1"
                     /translation="FSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKI
                     AKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQ
                     CLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQ
                     GNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLL
                     RKH"
BASE COUNT          237 a          129 c          120 g          178 t
ORIGIN      
        1 tttagtgcaa ccctcatttc actcatgacg gaagatttca cattggatga acttgctgag
       61 cttcctttag aagctttctg tgatttactt caagaaaaag gaaaaggccg tttcaagcaa
      121 ccagaaaaaa tcgctaaagc aattcaacgt gcaattacca tgagttaccg cctaggtgct
      181 cttgcacaag aatcaattaa tgttgtttta agtgttctcg tacgagaaat acgagcactt
      241 gaaaaaaata tcaaagaatt agataaagcc atcgaacaag taattgttgt cattccagaa
      301 tatcaatgct taaccagtat tccaggcgtt ggtaaagtct atgctgccgg tttaatcgct
      361 gaaattggtc aaatcgaaag atttgaagat cagacaaaat tggcaaaata cgctggatta
      421 agttggaaag tgaaccaatc tggaaactat cagtcgcaaa atacacctct gacaaaacaa
      481 ggaaatcgat attttagata ctacttagtt gaagctgcca actctgtaaa aaattatctt
      541 cctgaataca aagcttttta tcaaagtaaa tataaggagg ttccaaagca tcaacacaaa
      601 cgtgcactcg tcctaaccgc aagaaaattt acgcgtctgg tggatacgct actacgtaaa
      661 cacc
//
LOCUS       DACEOM010000213          628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEOM010000213 DACEOM010000000
VERSION     DACEOM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.224"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..398
                     /locus_tag="IW781_003062"
     CDS             6..398
                     /locus_tag="IW781_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1184009.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..>628
                     /locus_tag="IW781_003063"
     CDS             399..>628
                     /locus_tag="IW781_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1184010.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIY"
BASE COUNT          252 a           44 c          109 g          223 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataa
//
LOCUS       DACEOM010000214          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEOM010000214 DACEOM010000000
VERSION     DACEOM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.225"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(318..581)
                     /locus_tag="IW781_003064"
     CDS             complement(318..581)
                     /locus_tag="IW781_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ1184011.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACEOM010000215          588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEOM010000215 DACEOM010000000
VERSION     DACEOM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.226"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(65..150)
                     /locus_tag="IW781_003065"
     tRNA            complement(65..150)
                     /locus_tag="IW781_003065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="IW781_003066"
     tRNA            complement(167..238)
                     /locus_tag="IW781_003066"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="IW781_003067"
     tRNA            complement(250..322)
                     /locus_tag="IW781_003067"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="IW781_003068"
     tRNA            complement(340..421)
                     /locus_tag="IW781_003068"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="IW781_003069"
     tRNA            complement(429..501)
                     /locus_tag="IW781_003069"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="IW781_003070"
     tRNA            complement(505..577)
                     /locus_tag="IW781_003070"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACEOM010000216          578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEOM010000216 DACEOM010000000
VERSION     DACEOM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.227"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..476
                     /locus_tag="IW781_003071"
     CDS             <1..476
                     /locus_tag="IW781_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ1184012.1"
                     /translation="RGYNSTKNAYFYGLKIHIIVTKTGFPVTYSITDPSVHDVKVLET
                     LSEEANLPNILGDKGYVSHKIHEKLALKGITVLVPSRKNMDKLGKIDHRLLTRQRKAV
                     ETVFSSLERLGFQNFKSRSIQGLESKFESILLSYSILLSRAQQHFEGTLRYSLGY"
BASE COUNT          203 a           94 c          105 g          176 t
ORIGIN      
        1 gtaggggcta taactcaacg aaaaacgctt atttttatgg tcttaaaatt catattatcg
       61 tcactaaaac aggctttcca gttacttatt caatcacgga tcctagtgtt catgatgtga
      121 aagttttaga gactttatcg gaagaagcaa accttccaaa tatccttgga gataaaggct
      181 atgtcagcca taaaatccat gaaaagcttg cccttaaggg cattacagtc ttagttccgt
      241 cacgaaaaaa catggataaa ctagggaaga tagaccatcg attacttact agacaaagaa
      301 aagcagtaga aactgttttt tcttctctcg aaagactagg ttttcaaaat ttcaaatcac
      361 gttctattca agggctagaa agtaagtttg aaagcatatt attatcgtac agtattcttt
      421 taagccgagc acagcaacat tttgaaggga ctttgagata ttctttaggg tattaaaatt
      481 aactagcacc acgggtatat ttttattata cacagcttgt cgcttggata gaactttagt
      541 ataattaaac gaaaaagagg taaatgaaat ggaaatgt
//
LOCUS       DACEOM010000217          576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEOM010000217 DACEOM010000000
VERSION     DACEOM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.228"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          187 a           81 c           81 g          227 t
ORIGIN      
        1 ttttttcttg atgtgatatc cttgtctttt ttgtaatgtt tttaacttat tgcttttgca
       61 aaatttaatt acctgttttc tggatcaacg atagtgtaca ttatgctatc cttgtttgga
      121 ttttaggacg tttaaagagg cactttttta cataacttaa tccatagacc ttttaagcat
      181 gctgtgagat aataataaag aagagtttaa agcgcacccc aaaccacttc cccataagtg
      241 tattacgctt taaactcttt tatatttgaa ttcactaaga agcataccat atttttgaaa
      301 aaaagtgaga aaaaaggctt ataattagga tttagagtaa ttaattagat gtttatttta
      361 taggactgat actatagatt acaaatagta tcgtatttcg caatcttaaa attatcttct
      421 tactgtgaat aagataataa aatttgtatt tgtccttttt aggtaactta ttttaatcta
      481 tatacctttc tggatcaacg aaagtatact ttatatagtc ataacgccga tggtaactat
      541 gcactgtaac gataatgctg ctaaaatatc ttttta
//
LOCUS       DACEOM010000218          548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEOM010000218 DACEOM010000000
VERSION     DACEOM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.230"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            269..490
                     /locus_tag="IW781_003072"
     CDS             269..490
                     /locus_tag="IW781_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1184013.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKN"
BASE COUNT          196 a           85 c           87 g          180 t
ORIGIN      
        1 ccagcctctt tttatctgtc tttcactaat gatcagttgt agataagcaa gttgcttttt
       61 cttacatcat accgcccatc aagacaaacg tttttagctt gtacgtactg gacaaaaatc
      121 ccattatatc aagaaaaaac aacgttgata atacttaaaa ttattaggct agaacggact
      181 agtaagagtt ttttgcccct tttttgcccc tcaaatatcg ctttacagtc agaacataag
      241 ttcgtataat cgttttgagg tgatttttat gatggaagaa tttattagaa aaaatatcag
      301 tgatgaatat gcagattttt atgaacaaag caacgaaaaa gataaattcc agatggatgt
      361 ttcaatttta gctatattag ctttttccga aaataaacaa cctgtaactg caaaaaaaga
      421 aacggtattc tctgaaggca aaataaaaac tcgatatata ttagaggtag aaactaagtt
      481 taaaaactga ttggagtaat ggttatgctt tttaatgaga cacaattatg gtttaaattc
      541 gatccttc
//
LOCUS       DACEOM010000219          533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEOM010000219 DACEOM010000000
VERSION     DACEOM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.231"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..>533
                     /locus_tag="IW781_003073"
     CDS             37..>533
                     /locus_tag="IW781_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1184014.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          196 a           99 c           87 g          151 t
ORIGIN      
        1 taacgacaac caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt
       61 gtgcaaactc tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa
      121 gcgataaaca cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat
      181 cgcactggtt ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc
      241 gaatatggtg aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa
      301 actttaccag cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc
      361 gaaaaaggtg ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac
      421 tatactccac aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt
      481 aaatccagag ccttaaatga taagtatgtc gctattttta tggacgctac tta
//
LOCUS       DACEOM010000220          524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEOM010000220 DACEOM010000000
VERSION     DACEOM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.233"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(46..130)
                     /locus_tag="IW781_003074"
     tRNA            complement(46..130)
                     /locus_tag="IW781_003074"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Leu,seq:caa)
     gene            complement(148..219)
                     /locus_tag="IW781_003075"
     tRNA            complement(148..219)
                     /locus_tag="IW781_003075"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Gln,seq:ttg)
     gene            complement(229..301)
                     /locus_tag="IW781_003076"
     tRNA            complement(229..301)
                     /locus_tag="IW781_003076"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(266..268),aa:His,seq:gtg)
     gene            complement(363..447)
                     /locus_tag="IW781_003077"
     tRNA            complement(363..447)
                     /locus_tag="IW781_003077"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(411..413),aa:Leu,seq:caa)
BASE COUNT          148 a          135 c          123 g          118 t
ORIGIN      
        1 aaaatatagc aactctctat aagaaagttg ctatattgat aattataccg gcggccgggg
       61 tcgaaccggc acgtcctcaa cggacactgg attttgagtc cagcgcgtct gccaattccg
      121 ccacgccggc aaatttaaaa ataataactg gggtagctgg attcgaacca acgcatgagg
      181 gagtcaaagt cccttgcctt accgcttggc tataccccaa gaataaaggg cgaatgatgg
      241 gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg
      301 ccatcataaa agatgttatt taatttctca ataaaaaact aagatgaaga catcttagtc
      361 aataccggcg gccggggtcg aaccggcacg ccctcaacgg gcactggatt ttgagtccag
      421 cgcgtctgcc aattccgcca cgccggcaag ttgatttatt agtactaagg cggtaaccgg
      481 atttgaaccg gtgatgaagg ttttgcagac ctctgcctta ccac
//
LOCUS       DACEOM010000221          515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEOM010000221 DACEOM010000000
VERSION     DACEOM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.234"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(107..>515)
                     /locus_tag="IW781_003078"
     CDS             complement(107..>515)
                     /locus_tag="IW781_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ1184015.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DACEOM010000222          513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEOM010000222 DACEOM010000000
VERSION     DACEOM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.235"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            145..402
                     /locus_tag="IW781_003079"
     CDS             145..402
                     /locus_tag="IW781_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1184016.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          215 a           65 c           71 g          162 t
ORIGIN      
        1 atttttctgt ccacgttcat tttactccaa agttttcctc atttcaaaag aaaatcacag
       61 aatacacgaa gaagcgtttc tatttgtata aactatatca aagttctata cttaaattat
      121 caaataaaaa ggacgtgaag ataaatgtca aatttagaaa acaaagaaga aaaagttgta
      181 aacaaaattg tttctgttgt caataaactg gataaagagt tagacgaact tgatacgtta
      241 tctgaaaatc cagaaaaaaa acataaccta aaaaaatggt tagttgaacg aaaggctatt
      301 catgaaatta aaaaagttct tcatgaagct gataaatatg aaaaatatga tgaaaaagaa
      361 cttgataaag aatttaaaga aatcaatgac ttattactat aattgattta tacgactcat
      421 ttttgagtcg tcagactgca aacaacactc taaatttagg agtttgtcta cagtctgaat
      481 agattttaaa tttattgtcc ataatgttat tat
//
LOCUS       DACEOM010000223          489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEOM010000223 DACEOM010000000
VERSION     DACEOM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.238"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>489
                     /locus_tag="IW781_003080"
     CDS             <1..>489
                     /locus_tag="IW781_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1184017.1"
                     /translation="DILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESHASDIYLIV
                     EEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLTRHTTDVPD
                     KYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFN
                     GR"
BASE COUNT          167 a           92 c           81 g          149 t
ORIGIN      
        1 agatattcta cagtttcaat tccctaacaa acagaggtat aaaattgttg ggagtattcc
       61 ttaccattta agcacacaaa ttattaaaaa agtggttttt gaaagccatg cgtctgacat
      121 ctatctgatt gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg
      181 gttgctcttg cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt
      241 tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt acccgccata ccacagatgt
      301 tccagataaa tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata
      361 tcgtcaactg tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa
      421 tttaagtacc gttacttatg agcaagtatt gtctattttt aatagttatc tattatttaa
      481 cgggaggaa
//
LOCUS       DACEOM010000224          477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEOM010000224 DACEOM010000000
VERSION     DACEOM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.denovo.239"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            70..153
                     /locus_tag="IW781_003081"
     CDS             70..153
                     /locus_tag="IW781_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1184018.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            278..>477
                     /locus_tag="IW781_003082"
     CDS             278..>477
                     /locus_tag="IW781_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1184019.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLS"
BASE COUNT          198 a           63 c           72 g          144 t
ORIGIN      
        1 tttgtgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      121 cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga
      181 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
      241 atttatttac agatgtaata acttcaggag tgattacatg aacaaaaata taaaatattc
      301 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      361 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      421 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatc
//
LOCUS       DACEOM010000225        54907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.009, whole genome shotgun sequence.
ACCESSION   DACEOM010000225 DACEOM010000000
VERSION     DACEOM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.009"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            401..1924
                     /locus_tag="IW781_003083"
     CDS             401..1924
                     /locus_tag="IW781_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1184020.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            2161..3501
                     /locus_tag="IW781_003084"
     CDS             2161..3501
                     /locus_tag="IW781_003084"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1184021.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3654..4391
                     /locus_tag="IW781_003085"
     CDS             3654..4391
                     /locus_tag="IW781_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1184022.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4556..4777)
                     /locus_tag="IW781_003086"
     CDS             complement(4556..4777)
                     /locus_tag="IW781_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1184023.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4808..5839
                     /locus_tag="IW781_003087"
     CDS             4808..5839
                     /locus_tag="IW781_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1184024.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5826..7229
                     /locus_tag="IW781_003088"
     CDS             5826..7229
                     /locus_tag="IW781_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1184025.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            7295..7930
                     /locus_tag="IW781_003089"
     CDS             7295..7930
                     /locus_tag="IW781_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1184026.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTLTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7979..8659
                     /locus_tag="IW781_003090"
     CDS             7979..8659
                     /locus_tag="IW781_003090"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1184027.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            8794..10023
                     /gene="rpsA"
                     /locus_tag="IW781_003091"
     CDS             8794..10023
                     /gene="rpsA"
                     /locus_tag="IW781_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1184028.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            10205..11515
                     /locus_tag="IW781_003092"
     CDS             10205..11515
                     /locus_tag="IW781_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1184029.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            11758..12033
                     /locus_tag="IW781_003093"
     CDS             11758..12033
                     /locus_tag="IW781_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1184030.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            12268..13314
                     /locus_tag="IW781_003094"
     CDS             12268..13314
                     /locus_tag="IW781_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1184031.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            13316..14575
                     /locus_tag="IW781_003095"
     CDS             13316..14575
                     /locus_tag="IW781_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1184032.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            14581..15162
                     /locus_tag="IW781_003096"
     CDS             14581..15162
                     /locus_tag="IW781_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1184033.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(15244..16119)
                     /locus_tag="IW781_003097"
     CDS             complement(15244..16119)
                     /locus_tag="IW781_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1184034.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            16267..16599
                     /locus_tag="IW781_003098"
     CDS             16267..16599
                     /locus_tag="IW781_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1184035.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            16711..17919
                     /locus_tag="IW781_003099"
     CDS             16711..17919
                     /locus_tag="IW781_003099"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1184036.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            18073..18528
                     /locus_tag="IW781_003100"
     CDS             18073..18528
                     /locus_tag="IW781_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1184037.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            18890..19165
                     /locus_tag="IW781_003101"
     CDS             18890..19165
                     /locus_tag="IW781_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1184038.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            19178..21256
                     /locus_tag="IW781_003102"
     CDS             19178..21256
                     /locus_tag="IW781_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1184039.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            21416..23302
                     /locus_tag="IW781_003103"
     CDS             21416..23302
                     /locus_tag="IW781_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1184040.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            23314..24261
                     /locus_tag="IW781_003104"
     CDS             23314..24261
                     /locus_tag="IW781_003104"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1184041.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            24281..24808
                     /locus_tag="IW781_003105"
     CDS             24281..24808
                     /locus_tag="IW781_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1184042.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(24871..25524)
                     /locus_tag="IW781_003106"
     CDS             complement(24871..25524)
                     /locus_tag="IW781_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1184043.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            25656..26498
                     /locus_tag="IW781_003107"
     CDS             25656..26498
                     /locus_tag="IW781_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1184044.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            26656..27552
                     /locus_tag="IW781_003108"
     CDS             26656..27552
                     /locus_tag="IW781_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1184045.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            27555..28268
                     /locus_tag="IW781_003109"
     CDS             27555..28268
                     /locus_tag="IW781_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1184046.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            28288..28806
                     /gene="msrA"
                     /locus_tag="IW781_003110"
     CDS             28288..28806
                     /gene="msrA"
                     /locus_tag="IW781_003110"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1184047.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            28803..29030
                     /locus_tag="IW781_003111"
     CDS             28803..29030
                     /locus_tag="IW781_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1184048.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            29182..29733
                     /gene="lepB"
                     /locus_tag="IW781_003112"
     CDS             29182..29733
                     /gene="lepB"
                     /locus_tag="IW781_003112"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1184049.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29816..30358)
                     /locus_tag="IW781_003113"
     CDS             complement(29816..30358)
                     /locus_tag="IW781_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1184050.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(30595..30993)
                     /locus_tag="IW781_003114"
     CDS             complement(30595..30993)
                     /locus_tag="IW781_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1184051.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            31253..32113
                     /gene="ylqF"
                     /locus_tag="IW781_003115"
     CDS             31253..32113
                     /gene="ylqF"
                     /locus_tag="IW781_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1184052.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            32106..32873
                     /locus_tag="IW781_003116"
     CDS             32106..32873
                     /locus_tag="IW781_003116"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1184053.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            32929..33786
                     /gene="dprA"
                     /locus_tag="IW781_003117"
     CDS             32929..33786
                     /gene="dprA"
                     /locus_tag="IW781_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1184054.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            33908..35986
                     /gene="topA"
                     /locus_tag="IW781_003118"
     CDS             33908..35986
                     /gene="topA"
                     /locus_tag="IW781_003118"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1184055.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            35955..37331
                     /gene="trmFO"
                     /locus_tag="IW781_003119"
     CDS             35955..37331
                     /gene="trmFO"
                     /locus_tag="IW781_003119"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1184056.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            37529..38434
                     /gene="xerC"
                     /locus_tag="IW781_003120"
     CDS             37529..38434
                     /gene="xerC"
                     /locus_tag="IW781_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1184057.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            38471..39019
                     /gene="hslV"
                     /locus_tag="IW781_003121"
     CDS             38471..39019
                     /gene="hslV"
                     /locus_tag="IW781_003121"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1184058.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            39033..40433
                     /gene="hslU"
                     /locus_tag="IW781_003122"
     CDS             39033..40433
                     /gene="hslU"
                     /locus_tag="IW781_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1184059.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            40451..41242
                     /gene="codY"
                     /locus_tag="IW781_003123"
     CDS             40451..41242
                     /gene="codY"
                     /locus_tag="IW781_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1184060.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            41324..42199
                     /locus_tag="IW781_003124"
     CDS             41324..42199
                     /locus_tag="IW781_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1184061.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(42331..42969)
                     /gene="plsY"
                     /locus_tag="IW781_003125"
     CDS             complement(42331..42969)
                     /gene="plsY"
                     /locus_tag="IW781_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1184062.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            43096..43530
                     /locus_tag="IW781_003126"
     CDS             43096..43530
                     /locus_tag="IW781_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1184063.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            43696..45741
                     /gene="parE"
                     /locus_tag="IW781_003127"
     CDS             43696..45741
                     /gene="parE"
                     /locus_tag="IW781_003127"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1184064.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            45753..48203
                     /gene="parC"
                     /locus_tag="IW781_003128"
     CDS             45753..48203
                     /gene="parC"
                     /locus_tag="IW781_003128"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1184065.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            48480..50711
                     /gene="pflB"
                     /locus_tag="IW781_003129"
     CDS             48480..50711
                     /gene="pflB"
                     /locus_tag="IW781_003129"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1184066.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            50955..51716
                     /gene="pflA"
                     /locus_tag="IW781_003130"
     CDS             50955..51716
                     /gene="pflA"
                     /locus_tag="IW781_003130"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1184067.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            51801..52727
                     /locus_tag="IW781_003131"
     CDS             51801..52727
                     /locus_tag="IW781_003131"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1184068.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            52909..53802
                     /locus_tag="IW781_003132"
     CDS             52909..53802
                     /locus_tag="IW781_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1184069.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            53936..54061
                     /locus_tag="IW781_003133"
     CDS             53936..54061
                     /locus_tag="IW781_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1184070.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(54195..54827)
                     /locus_tag="IW781_003134"
     CDS             complement(54195..54827)
                     /locus_tag="IW781_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1184071.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        18999 a         9344 c        11579 g        14985 t
ORIGIN      
        1 aatcaattct gattcatcag tcccataaat tatagagccg aaatttcttg gtcttttttt
       61 ttggaaaaaa atacaagaca tgcaataatg taaacgtttt atttgtttgt tttgagtatt
      121 tttttgattt atgcataaaa atcaatcaat tctttgcaaa tgtgaaattc ttctcctttt
      181 aaaagacgaa aatgtttgtt tataaaaaaa aggaaacgct acaatgaaaa agaagaaagc
      241 tttaaaaacc tgaaaaaaga ggagggaata gggaaaaacg aactttttga acaaaagttt
      301 tacaaaatgc gtgttattat ttttgttctg atactctttt tgataaaata acattagagc
      361 caaaattgtt gtctctaacg gaaaagaaag gaagaagaag atggaacaaa caaaaaagaa
      421 aaaatttcaa atgccttcag cgtatacggt cttatttatt attattgcag taatcgctgt
      481 acttacttgg tttataccag ctgggcacta tagtgtagac gatgcaggca acatcgtcgc
      541 tggatcatat gaacgagtac aacaaaatcc tcaagggctg tgggatattt tcatggcgcc
      601 aatcaatggg atgctaggtg taccagcggg ggagctaact aaagaaacac ctgctgcaat
      661 cccaatctct ttcttcattt tggttgttgg tggattttta ggtattatca acaaaacagg
      721 ggcactagat gccgggattg cttcagttgt gaagaaattc aaaggaaaag aaaaaatgct
      781 tattccagta ttgatgctgt tatttgcatt aggcggctct acttacggaa tggcagaaga
      841 aacgattcca ttctatgctt tactgatccc agtcatgatg gctgtaggct ttgatacagt
      901 agtagctgta gcaatcgtat tagtagggtc tcaagtaggc tgtctagctt caacggtaaa
      961 tccttttgca acaggcgtag cttcacaagc tgttgggatt tcgatgggtg aaggtattgg
     1021 tcttcgattc ttgatgttcg ttattttgtt agctatttct attgtatatg tgtatcgtta
     1081 tgcatcaaag atcgaaaaag atccaacaaa atcattagta tacaaccaac gtaaagaaga
     1141 tatgaagcat ttcgatattg aagcaatcgg tcgtcaagaa gtaatcacga aaaaacaaaa
     1201 acatgttaat gtattgttct ttattacttt tggtatcatg atcatcagtt tgattccttg
     1261 gacaaccttg aatgcgaatt tcacgatttt tgaatcattg acaaactggt taacaaacct
     1321 tccggtacta ggtacggttc taggtaaaag catgcttcca ttaggtgact ggtacttccc
     1381 agaaattact atgttattct tagtaatgtc catcgtagta gcaatcactt atggcatgaa
     1441 agagtctgaa tttgtctctg aatttttagc tggtgcttct gatttgatcg gtgtagcaat
     1501 cgtagtagct gttgctcgtg gtatccaagt ggttatgaac aatggattaa tcacagacac
     1561 catcttacat tggggcgaac aaggattaag ttcattatct tcaagtgtat tcatcattat
     1621 tacatttatt ttctatatcc ctatgtcatt cttgattcct tcaacttccg gcttagctgc
     1681 tgcaacaatg ggaatcatgg gacctatggg tgaatttgcc ggcgtcggta aggatttagt
     1741 aatcactgct tatcaatctg catctggttt agtaaactta attacaccaa catctgctat
     1801 cgtaatgggt gctgtagcaa ttgctcgttt cgatgtttca gtgtggtgga aatttaccgg
     1861 taaattaatt gcgatattat ttgtagccat ctgtgtgatc ctaggtgtag cagcaatgtt
     1921 ctaaacctag acaaatacag gtacaaggtc tatcataaga atgaagaaac cgataaacgg
     1981 ctatcgataa gcacgtggat aagtcaatag ctccattcag tgagtgtttt ttctttcatc
     2041 atttgttaag aacattctgc ttgctgatgg agcaggctta aaacgtcatt atttgagaag
     2101 aagaggttgt gtcggaggaa tttgacatga ccctttttaa catattagga ggataatatc
     2161 atgaaaaaat ttgtgacaga acaacatcat gaacaagcat tagaatcatt gagcgaattg
     2221 atccgaattc cttccgtact agatgaagca gattcaggac aaggacatcc cttcggtaaa
     2281 aaagtgattg aagctttgga taaagtattg gaaatcagtg aaaagctagg atttagaaca
     2341 tttaaagatc cagaaggcta ttacggttat tcagagattg gctctggaga tgaattattc
     2401 ggtatcttgt gtcatatgga cgttgttcca gcaggggacg aaaataattg ggaaacaaaa
     2461 ccattcgatc caactataaa agacggctgg ttagttggtc gcggatccca agatgacaaa
     2521 ggtccttcta ttgcggcaat gtatgccgtc aaagctttga ttgatgctgg cgtggaattc
     2581 aagacacgta ttcgttttat ctttggtaca gatgaagaaa atttatggcg ttgtttagaa
     2641 aaatataacg aaaaagaaga aggaattacg caaggatttg cgccagatgc tgaatttcct
     2701 ttaatttatg cagaaaaagg cttattgcaa gcttacttga caggaccagg aacgaacgag
     2761 ttttctgtaa aagctggagg agcgttgaat gttgttcctg atgcagctcc ttactctgga
     2821 gaaaagcttt ctgaagtaaa agaagcattg aaaaaacacg aattcgattt cgaagaacaa
     2881 ggtgaaggca tcgtcgttct tggtaaaagt atccacgcga aagatgcagc acaaggagtg
     2941 aatgctattt ctcgtctggc aattgctctt tcagaagtat ttgattttgg cccaatcaat
     3001 ttcttaggaa aattagttca agaaaatgca actggtgaag ctgtcgtggg caaaacagaa
     3061 gatgaacaat ccggcgagct aacgatgaac tttgcaagtt tggaaatcac accagaacaa
     3121 acaaaaatcg gtgtagatat gagaatccct gttacattca agaaagatga tttagtagct
     3181 aaattaacta aaacagcaga aaaatatggg ttgacttatg aagaatttga cttccttgat
     3241 tcattatacg tgccactaga cagtgagtta gtgaaaaatc tattggggac atatcgcgat
     3301 attactggag acatgactga accgtttgta tcaggcggcg ctacttttgc tcgtacgatg
     3361 aaccagtgtg tggcattcgg tgcgatgttc cctgatactc ctgactttat gcaccaggcg
     3421 aatgaacgat gggaattaag tagtatgtat aaagcaatgg aaatttatgc agaagctgtg
     3481 tatcgtttat gtgctaaata aaaataaaca tacaaaaagg gcggatattc aaatggatat
     3541 tccgtctttt tttatttgat gctggaaagt ttgcatttct ctcttatttt tttaaggtat
     3601 aatcatactt gattaagaaa aaggggtcac tcagatagaa aaggtgggag aaagtgaaaa
     3661 tatttagacg aatactaatt ggcttagtca tcattttagg attactcggt ggagccggtt
     3721 attactatat acagcaaaat acgtacgaac catcgtcaga agcgcaagtg ttggtaaaag
     3781 aaggaaaaaa caaagaagat tacttatttt ttgaaggaga gaaaaagaat catggaaagt
     3841 ctccattact tattttttat cctggtgctc tcgtagagcc agaaagttat agtatttggg
     3901 cgcaagattt agcaaaaaac ggcttttcag tcgctattgt caaaatgcca cttgatctag
     3961 cagtgatggg cggaaacaaa gcggataagg tcaaagaaga ttttccaaat tccgattatg
     4021 tgatcggtgg ccattcatta ggtggcgtaa tggcaagtcg ttatgtcaat aagaatcatt
     4081 cagacaaaca tttaaaaggg gtattcttca tggcaagtta tcccgacaaa aaaggaacat
     4141 tgacagaatc agctgttcct gttttatcga tcacggcgac aaaagataaa gtattgaatc
     4201 aggaagctta tcaagaagct aagcagtatt taccggaaag aacagagtat gtatcaatca
     4261 atggcggaaa tcatggaggt tttggcagct atggtgagca aaaaggagat ggagaggcag
     4321 aaatttctaa cgcagaccag caaactgtga tcgtcaatac attggaaaac tggctgaaaa
     4381 agttgaaata atataaatgg gtcctatagg gtccgtgaaa aaactagtca attaactata
     4441 gatagcaaat gaatctttca aaataagtcg ggatttcacg gaacaagaga aacagatccc
     4501 ggcttatttt caagtttaga gaacttagtg tacgttttta aagtttcgtt tatatttata
     4561 ttttattttc taataaaatc gctcgaactg gacatttttg atacgctttt tttacatatg
     4621 tttcttcttc cggcatcacg aactgttgat gagcctctgg ttcttgggca aataaaacga
     4681 ttccttcttc atcataatcg aaaatatctg gcgcttcgat ttgacaaaga ccgcatgcaa
     4741 tacagcgttc aggtacaagt ttacatagta gtgacatttc taaccatcct aacttaagga
     4801 gaataatatg attgttttta ttttatcgct attttcatca ggacacaagc tgcggattag
     4861 ttctctttac caactccttg tgggaaaacg aacgacttca gtacttattt atggatttac
     4921 ccacgaactt ttgtttatcc ataacagttt tccagaattg aagcaggata agttttatca
     4981 aattttgcaa aaaattgcgc agcaaggttg gatcgaaata aatgaaaatg aagcaaagct
     5041 aacgcctgca ggggctgata tgttgtccga acatcaaata gaacatactg gtttgcgttt
     5101 tgaccgctat ggcagaacag gggaaactag ttggcggctc attaaatttg ctgttcaagt
     5161 aatttctaat ctggcaacgg gtattcaaga ctatcttcca gcagagacaa gtccttttta
     5221 tactttccaa ctaaaaaaat ggcttagtga aagccaattg ccacgtgaaa ttttgattga
     5281 tactgcgtat gagagtttgg tgcagctgtt ttcggagata ccagaagaag cagcagattt
     5341 tttagctaac caattatctg gaaatgagcg aacagggctg cttccttatc agctggcaaa
     5401 aaacaatgat gagtcagcgg tttatctgca acaaagcaga tgtatccacc ttttgttagc
     5461 acaaatcgaa aaacggccag attcgctttg gcacgctttg atcgattcat tattacagca
     5521 aaatttcaat caaagcatga tgatcaccaa gcagatgttt atgaatggtc agacaattga
     5581 ccagatcatg gcaatccgtc atttaaaaag aggaacagta acagatcatc ttattgagtg
     5641 ggccttgttt tttgatgatt ttccatacga gtggattctc tctgcagaaa caatcgaacg
     5701 tttggaacca aataaggatt ccgtgcgaga gtggcgcttt agcgaatgga atgtagatgg
     5761 acaattagat tatggagaat ttcgtttgta tcaaatctat ctattgagaa aggaagcgat
     5821 acagaatgtt aataaatgaa ttaaaaaaat attttggatt tgattcattt cgtcccggcc
     5881 aagaagaaat tattcaggcg ttgcttgatg gagaagatac tctcgctatt ttgcctacag
     5941 gaacgggaaa aagcctttgc tatcaattga cggggtattt aatggaaggt ctggtcatca
     6001 tcgtgtcacc gctgctttct ctaatggaag atcaagtgac gcaattacaa aaacgagggg
     6061 agaaacgagt agctgctttc aatagtttac tttcgcaatc tgaaagaaga tacgtcctcc
     6121 gtcatctatc gcaatataaa tttttgtttt tgagtcccga aatgttgact aatccttctc
     6181 ttttagagca tctgaaaaaa caaaatattg cgttatatgt agtagatgag gctcattgcg
     6241 tgtctcaatg gggagttgat ttccgtccag aatatcaaca gttaggaaaa atccgaaagc
     6301 ttttaggaaa cccggtcaca ctggcactaa cggcgacagc aacagattta gtagcaagag
     6361 atatacgtca tgtactattt gatcgaccac caaaagagat cagacaatca gtcaatcggc
     6421 aaaatatctc gctttttgtt cggaaaacgc agcaaaaaga acaagaactt gaacacttca
     6481 tggaaagagc acaaggagcg gcaatcattt attgtgcaac aaaaaaagaa gtcgaacgat
     6541 tgtatcattt attcagggaa cgttttacag ttggatacta tcacggtggt cttgatgcag
     6601 ctcaaagaag acaacttcaa cagcagtttg taaaaaatca gctgcagttt ctcattgcta
     6661 cgaatgcatt tggtatgggg atcgataaat ctgatatccg ttatgtggtc cattatgatt
     6721 tgccagacag tctagaaaac tatgtacaag aaatcggtcg agcaggcaga gatcaggaag
     6781 aaagtgcagc aattcttttt tatcaaagtg gggatgaacg tatccattat ttctttaatc
     6841 aactttctag agaacagcga caaagttttg agctttattt ggaatatgcc acagagcaag
     6901 ctccctttga tgagctgcaa aaaaaatgga tggagctgat ccagcagtct gaaaaaccag
     6961 aaaattgggt ggaacggctc aaacgtcagg aaaaagaaaa agaattccgc ctgcagcaga
     7021 tgcttcgcta tattaatgaa gaaaactgcc gcagaaaatt catcttagct tattttggcg
     7081 aaaaactctc cgaaaagccg cagaattgtt gtgatattga tggagcaaaa gcagaacttc
     7141 tgccaaagag gaagcttttt tctaagactg aacaacttca ttgggaaacg attttactaa
     7201 atttatttaa aaaaaacatg gaacattgat tttttaggac aagacacttt ttcctatggt
     7261 ataatacaca cgatagagtt taggaggtaa aacagtgagc agaaaagata gacatcaatc
     7321 tacagaaaat aacgaaacac aagaaccatg ggaacaacca atctacgata cagatgatga
     7381 agtttcatca agaagttctc agagacaaca aaagaaagga aatacattat ttctttcgct
     7441 atttttgatt ttactggtct tatgtatcgc tattccagca ggtgcttatt tctggatcag
     7501 aaatggatca agcaacaatg caacagcagc aacttcttct gaaacttcga catcagtagt
     7561 agaaagttct acaagtacaa caaaagagga gtcaactgag acttcaacag tagaatctag
     7621 tacaatcgcg gaagaatcta ctgtgtctca agatacaggt gtaacgtcag aaagcagtat
     7681 tgcagcagag acaacactga cttcctcaag tgaggcaact tatcaagcag gggattcaag
     7741 cagcaatgca gcagaaacaa caccttcttc atcagctgca gccggttcaa caacgactgt
     7801 tcaagcaggc gaaggtccaa aacaagtggc tgagcgtgca gggatcacca ctgatcaatt
     7861 gtttgaattg aatggcttag atccaaacaa ctttatgctt taccctggtc aagaattaag
     7921 aattaaataa taatagccgc gctttgcggc tttttttctg agaaaaagga gttatatcat
     7981 gacacaaatc agtattgcaa tcgacgggcc tgcttcatct gggaaaagta ccgttgccaa
     8041 gatattagca aaagaattga attatattta tacagatacc ggtgccatgt atcgcgcagt
     8101 aacttattta gctattacga acaatgtatc tttttcagat gaagaagggc tagtttcgct
     8161 gatcaaaaag tatcctatct ctttttcgca acaagaagat ggacagcatg tattcatagc
     8221 atcaaaagat gtgactttag aaatccgtca accagatgtc acaaaagctg tttctgaagt
     8281 ctctgcacat ggaaaagtca gagaagaatt agttgctgtc caacgaaaaa taggggaacg
     8341 cggtggcgtt gtcatggacg gaagagacat cgggacagct gtattaccga atgcagaagt
     8401 gaaaatcttt ttagtagcaa gcgtaagtga gcgtgcagaa agacgtttca aagagaacca
     8461 agaaaaagga attccgactg atcttgaaac actgacgaaa gagatacagg aaagagacca
     8521 ttatgattca acaagggacg tctctccgct gaagcaagca gaggatgctg ttcgtatcga
     8581 tacgactggc aaatcgattc cagaagtcgt tgcagcaata aaagctgttg ttttgaaaaa
     8641 aggttatctt cttttttaaa ttaagaaaaa aaatgaaaaa caattgtttt ttcaaggaaa
     8701 acgaacgaaa tcgctttatt ttttttgaat aatcaattaa actaaaaaca gtaccgtact
     8761 tacggcagaa tgttggttag gaggacaagt gttatgacag aatttgatca aaatcaagag
     8821 aacaaccagt caatggaaga cgcaatgaaa agcgtacaag aagtaaaagt tggcgatgtt
     8881 gttaagggcg aagttttagt cattgaagac aaacaagtga ttgtcggaat cgaaggggct
     8941 ggagttgaag gagtcgttcc ggcaaaagaa ttatcaactt tgccggtaga agatatcagc
     9001 gaagtagtaa aagtcggtga tgtattagat ttagtcgtta tcagtacgat cggcaaagat
     9061 aaagaaaacg gcagctatct actttcaaaa cgccgtttag atgctaaaaa agtttgggaa
     9121 gatatcgaaa gagatttcca agagggcaag gtcatcgaag cacctgttac aaatgttgtc
     9181 aaaggcggtt tagtcgttga cgtcggtgta cgtggttttg tccctgcatc aatggtggaa
     9241 gatcattttg ttgcagactt ttcagactat aaaggcaaaa cattgagctt taaaatcatc
     9301 gaaatcgaac catcagaaaa tcgtttgatc ttatcgcata aagcggtagt ggaaaacgaa
     9361 agagaagaaa agaaaaaaga aattctttca actatccatg agggagatgt tctcagtgga
     9421 cgtgttgcac gtcttactga ttttggcgca tttgtcgacc taggcggaat tgacggtcta
     9481 gtccatgtct cagagatttc acatagtcat gtagcaaaac caagtgatgt attggctgtc
     9541 aatgatgatg taaatgtcaa agtgttgtct atcaatcctg aacaagaacg tgtttccttg
     9601 tcaatcaaag acacacagcc tggaccttgg tcagaaatcg atacaaaagc tccagttggc
     9661 acagtattag acggaacagt taaacgtttg acaagcttcg gtgcctttgt tgaagtgttc
     9721 cctggagtag aagggctagt ccatatctct caaatttcac ataaacatat tgcgacacca
     9781 catgaagtgc ttcatgaagg tgacgaagtg aaagtgaaag tattagagat ccatccagaa
     9841 gaacatcgta ttgcattgag tatcaaagct ttagaagaaa aaccagcttc agaagaagaa
     9901 ccaaaaaatg ttgagtctta tgaattacca gaagaaaata ctggatttac tatgggagac
     9961 attttaggtg atgcgttgaa agaagaagta gaagaaaaag caaccgatga ttctgaaaaa
    10021 taattgattt tgtatctgtg actgagatga aagcagctgc ttttgtctca gtttttcttt
    10081 tgaaaagcga ttagtatttc aataaggaat aaaaatactt cgtttaattg acaatctatg
    10141 cttgaaagtt ggcaatgcat tcgttaaact aagtgagatt ggaaacttgt gaggaggaaa
    10201 taatatggca aatccaacaa ttgcaatcgt tggccgcccg aacgtcggta agtctacgat
    10261 ttttaaccgc atcgctggtg agcgtatctc gattgtcgaa gatactcctg gagtaactcg
    10321 tgatcgtatt tatgcaaaag gtgaatggtt aggtcgagaa ttcagcgtga tcgacacagg
    10381 cggaattgat cttggcgatg aaccttttat ggatcagatc aagcatcaag cagaaatcgc
    10441 gattgaagaa gcagatgtaa tcatttgtgt agtcagcgga cgcgaaggcg tgacagacgc
    10501 agatgagatg gttgcgaaaa ttttatacag aagtaataaa ccagtgattt tagcagtcaa
    10561 taaagtagat aatccagaaa tgagaaacga tatctatgaa ttttattcct tagggctagg
    10621 cgatccttat cctgtttcag gaagccatgg actagggatt ggtgatattt tagatgaagc
    10681 agtgaagtat ttctctgaag agactgaaga agaggacgac gacacgatta agtttagttt
    10741 gattggtcgc ccaaatgttg gtaaatcctc tttgatcaat gcgattttag gagaagaacg
    10801 tgtaattgtt tcggatattg aaggaacgac ccgagacgca atcgacaccc acttcgtttc
    10861 tgaaagtggt cagaaattct taatgataga tacagctggt atgcgtaaac gcggaaaagt
    10921 atacgaaaat actgaaaaat acagcgtgat gcgtgcaatg cgtgccattg accgttctga
    10981 tatcgttttg atggtactga atgctgaaga agggattcgg gaacaagaca agcgagtagc
    11041 gggttacgcc catgaagctg gtcgtggaat tattatcgtc gtgaataaat gggatcttgt
    11101 aaagaaagaa accaatacaa tgcgtgattt cgaacaagag attcgtgatg aattccggta
    11161 tcttgattac gctccaatcg tttttgtttc tgcagtgact aaacaacggt tggaacgtct
    11221 gcctgaaatg atcgaacaag taagtatgaa ccagaatctt cgtatctctt cggctgtgtt
    11281 gaatgatatc attatggatg ctgtagcaat caatccaaca ccaacagata aaggaaaacg
    11341 cctgaagatt ttttatgcta cacaagtcgc tgtcaaacca cctacatttg tcgtttttgt
    11401 caatgaagag gaattgatgc atttttctta tgaacgtttc ttagaaaatc aaatcagaaa
    11461 agcatttact tttgaaggga cgtccatccg aattatccct cgtaggagaa aataggcatt
    11521 ttaccatatc gacgtttttt tttcaaaaga aaaagacagt ttcgtttgtt acgtttctat
    11581 gactaaaaaa agcatgtgaa aattggtaaa aaccttgcta ttaagcggtt catatgatat
    11641 cgtgataaca gaatattgaa gttaatcaat atttcttttt aaacttctgg accactcaga
    11701 aagttttaac taaatgatat ttctatcata tctcttgtgg aggaggtgaa tctatccatg
    11761 gcaaacaaag cagaattaat cgaaaaagtt gcttcagcta ctgatttaac taaaaaagat
    11821 gcaactgcag cagtagatgc tgtattttca actattcaag atgctttagc taacggcgaa
    11881 aaagttcaat taatcggttt tggtaacttt gaagttcgcg aacgcgcagc tcgcaaaggt
    11941 cgtaacccac aaactggtga agaaatcgaa attccagcta gcaaagtacc tgcattcaaa
    12001 ccaggtaaag cattaaaaga cgctgttaaa taagacagtt gaaaaagcgc ttgtggctct
    12061 agggctacaa gtgcttttct ttttatttcc tagcagaaaa ggaataaata agagacagag
    12121 attccgaatc cgtatgtcat acatagaaaa cacatgtatt ttatttaatt tttatttatt
    12181 tgcaagttat gaggtgagct ttgctcttat cttttctaga atttgcttac taatggtaaa
    12241 ataaaaataa gaagaggagg gatgcgtatg gaacttgatt atcaagtaga agatgcgttg
    12301 acgaaagtgt tttacggaga aacgctagtc agtcagctaa gagaactttc cattgatcag
    12361 ccaatcttat ttttaacgaa tcagcgctat tatgatttgt ttgcagataa gatcaacccg
    12421 ttatttgcaa atcaagcaaa caatgactgg tatatttgtg ccaacactca atgcaatcat
    12481 ttaacagaat taaagaacct gcttgatttc gtcaaacgct atcccgaaaa tcaatccatg
    12541 tttatcatcg gatttggaaa tgaagggatc atggatctgg caggtttttt tcaaaaacat
    12601 acaacccttg attcatcact atggatgatc cctgtatcga tccgttctct aagccgagca
    12661 ttaacaccgc agcggacgat tttgcagcta ccaaatcgtg tagtgctgca aaccatcaat
    12721 ctgccagaac gcatcgttta tgatcaaaca atcagcgagc ggcaggtgga cggaaagcag
    12781 attgactttt tgacttttat ctgttgcgga atgatttgtg atcatcgttt tttgcaaaat
    12841 ctgtataaaa actatccaaa taaaaagcaa ttaacgactc ggccgtttgc cgggatgatc
    12901 gaagaaatga tctattttta tcaattagag gcagaaaacc tgtcaaatta tggtaaactg
    12961 tttgaacaag ctttttatct gaccgaaaat ggccatctac tttctgctag tatgaaaaaa
    13021 tttttaggaa tgatgtttca tttcgtgtgg aatctccaag catatccttt tgatttccaa
    13081 ctaaaaaact tttttgtgtg gctggacttg cttggctatc caatccactg gctggaacag
    13141 atttcaaaga tggagtattt ggaaaaagtt ctttttttgg cagaaaaaag caaaaaaata
    13201 cttgttttag aaaaaatagg tataatagac ggataccaac atccaaacga aaaagaatta
    13261 ttgagaacaa tgacggtatt tcaacaaatc gtcagtgaga taagagggaa ttgaaatgaa
    13321 tacaaatagc gaaaaaatgt tgcaagcttt atcagatgaa gacttgtcac aagcacaaat
    13381 ctttttagaa aaagcattaa aagaagaccc agcagatata ttagctgaac taggcgaaga
    13441 attgctggct attggctttt tgatcgaagc aaaacagatt tttgaacaac tgatcaacca
    13501 atacccagaa aatgacggac tgaacatccc gttagcagaa attgcaatcg aagataatga
    13561 gatcgactta gcttttgaat acctggaaaa agtggataag caaagtgatt actatccgca
    13621 aagcttattg gcgatagctg atttgtacca agttatcgga atccctgaag tgagtgaagc
    13681 aaaattgaaa gaagcagctg ggttgcttcc tgatgaacca ttgatccagt ttgcgctagc
    13741 tgaattgtat ttttcagttg atcgtttcaa agaggcagca gtggtttatg aaacattgtt
    13801 ggcaagcggg ataaatgaaa tttcaggaat ctcgatgcag gaacgtttag gacaatcctt
    13861 aagtatgcaa ggagaattcg aagcagctat cgtaccttta gaagaagcac tgaaagaaga
    13921 aaggacagat gatcgactgt tccagctagc ttttacgaat cttcaattga aagaaaatca
    13981 gcaggcaatc aactatctgg aagaacttcg agaagttaat ccgcattatc aatcgttata
    14041 tctatatcta ggacaagctc ttcaagaaga agaaatgatt gaagaagcac aaacagtgtt
    14101 agaagaagga atcaaagaaa atccttacca agtcgaactt tatcacctgg cctcggaaaa
    14161 tgctttccgt ctgcatgata gacagcgagc agaagaactt ttactgaaag ctttagaagt
    14221 aggagacaaa caagatgaaa cattacttac tttaagtaat ctttacttgg atgatgaaag
    14281 atatgaagat gtgatacaag tgatcaatca gatggaagaa actgctaatc cttatgcaga
    14341 atggaaccta gcccatgctt ataatgaatt agaagacttt accgttgcag ctgtacacta
    14401 tgaacaggct tatcatgaac tatcacatga accagatttc ttgaaagagt atgccttttt
    14461 cttaagagaa gaagggcagc tgaaaagagc gcaagaatta ttgacttatt atttaacact
    14521 tgaaccagga gatatggagg cactgtcatt attagatgac ctaacagaga ggtgagaaaa
    14581 atgctaattg atgtacggga aaagaagaat tttttaacgt ggatggtcaa tcatgtatct
    14641 tttagccgaa gagaagtttt ttggatcttg aattatcttg ccaatcatga agcgatttta
    14701 agcaatgtcc actttgttga aggggcgaat tttgcggatc gaggactgca gatcagtgat
    14761 ctatctgttg aaggggaacc catgaaattg tttttgcagg gaaaagaatt tacggacaca
    14821 gaccagattt ttcatgaaat ccgtttgaat tggaaaaagc ctttatatgt ggaatgtata
    14881 ttcgaaaatg cttggcagac aagagaatat ctatctatct tagaagacaa tccattttct
    14941 tcttgggatt cttcagtcag tgaagaagat actgaattaa ttgaacgata ttttcaagaa
    15001 aaagaagaag aggcaaaact aaaaatgctt tatgctcaaa tcgatcaagc actagaagat
    15061 ggagataggg atgctttttt gaaattatct gatgaagtta accgaaagat ccttttgcag
    15121 acacaaaaga aaagtaaccg ggtaagtgaa cataacaatt agttaaaaat agtatagtac
    15181 taaaaaagac aaaggtcagc tggatgtgga atctttcctc atagcctatc tttgtctttt
    15241 ttcttatagt tttttcagta atcttgtctg tggcttcaaa taagtaaagc cttgcccttc
    15301 cacttcatgc acattgatga ttgaaaggaa agctttttcg tcaatctcgt ggacgatcct
    15361 cttagcttca gttaattcac tagagctaac gaccatatag atgatttttt ttgaattacc
    15421 agagaatgcg ccttctccag aaaggaaagt cacacctctt tgcagaccgg tcatcagtag
    15481 tggtgcaatc tcttcgcttt tgttggagac gacaagtaga ccttttgcag aatatccgcc
    15541 atctaagatc gaatcaatca cacgactgaa aacatacgaa acaatcaacg tatacatcat
    15601 acgtttcaag tcgatatagg ttaatgaagc aagtaagacg atgatatcaa atgccaatag
    15661 ggaacgcccc atagtgatgc cgtaattttg ttctaaaatc cgagcgataa tatcactgcc
    15721 gcctgttgtt ccacccatgc ggtagacgat cccgcttcct acacctgcaa ctaaacccgc
    15781 aagtagagaa acgatcagta gatcatgctg taaattgatt tctagtggaa ccctttgcca
    15841 gatccataaa aagaatgaca aagaaagtgt acctagaaca gtataggcca aggaacgttt
    15901 accgaggatc tttcctccta gtaaaatcaa tgggatgttg attaataaag tcgtataagc
    15961 tggatcaata ccaaataaag ctcttaagat caaggtgatc cctgagactc ccccttctgc
    16021 taaatgatta gcgatgttca agtaaacaag accaaatgca taaatacttg ttcctataat
    16081 gataaaaaga acttctttta ctgtttttcg tttgaccata tgaattccag ctttcaatta
    16141 tgtattttca cagaatttcc atttagaaat tccttcctaa atgtagcatg gaaattcgat
    16201 tttctcaatt gctcttatct aggatttgcg ttaaagtttt ggtaagatag ttttggaggg
    16261 aaaaagatgg ataaaaagcg ttctttatac agtatgcagc aggaagtgga tgaatatatc
    16321 cagcaattca aggtggggta tttctctcca ttagctcaga tggctcgatt aactgaagaa
    16381 gtaggtgaat tggcaagaga agtcaaccac acttatggag aaaaaagcaa aaaggcttct
    16441 gaaccagtta actcggtagc agaagaactt ggtgatgttt tattcgttac gatgatcatg
    16501 gctaattcat tgaatatcga cttaactgaa gtttttgaaa aaaacatgga aaaattcaat
    16561 caaagagacc ataatcgctt tgagcgtaag gacgagtaaa caagatgaag tggtcctggc
    16621 cttgaagact ctgaaacgga cttgtcaaac agtcaccatg caactgagtc tttatctcag
    16681 gacaagaaac acgcaggaaa ggttggaaaa atgaaattaa aacaattacc tctggaatac
    16741 caaaaggcaa ttccagttct gaaaaaatta gagaatgcag gatatgaagc ttattttgta
    16801 ggtggaagtg ttcgtgatat cttgctgaac cagccaatcc atgatgtgga tatcgcaaca
    16861 agcgcatttc cagaagaaat caaacagctt tttccaaaaa cgatagatat tgggatcgaa
    16921 catggaactg tgttggtatt agaaaatgat gaacaatatg agatcaccac atttcggacg
    16981 gaatcaactt atcaggacta ccgtagacca gatcatgtcg aattcgttcg ttcgttggca
    17041 gaagatttga aaagaagaga ttttacgatc aatgcgtttg ctttgaaaga agatggagaa
    17101 atcatcgatt tgttcgatgg tctgcaagat ctagaagaac agatcttgcg agctgtaggc
    17161 aatcctcatg agcggttcca tgaagatgcc ttacgaatga tgcgcgggtt gcgttttgtc
    17221 agtcaactag ggttcaaatt agaggaagaa accttctacg ccatcaaaga aaaccacgag
    17281 ctattaggta aaatatctgt agaacgtata aatgtggaat tcatcaagat gctcttggga
    17341 aatgaacggg ccgcgggaat ccatgcattt attgaaacag agtgctacat ttgctgtccc
    17401 ggtttgaaag aagaaggcga agcactttta cgttttgctg aattaccatc cgtcccattg
    17461 aaagaagaga gccaagcatg ggcattattg atcagccaac tggatctaaa agaaaatgag
    17521 attcgttcct ttttgaaatc gtggaagtgt tctaaccaaa tgattaaaga cgttcaagca
    17581 cttgtccaag gactacgaca acgagaaact ggagctttgg aaccattcga attgtacttc
    17641 ttaggcaaaa aacatgcact gcaagtagaa gaattgatga tctattttga cgaaacacct
    17701 cagatggatg ccgtaggaga atcctatgat cgtttaccta tcaagtcatt aagcgaatta
    17761 gcagtagatg gtaaaatctt gctagaagaa actggaaaaa aacctggtaa gtgggttagt
    17821 gaagctttgc aattagctga aaaagccgtc gtagaacaaa gaataaaaaa tgataaaaaa
    17881 caggttttgg attttttatc gaaagaaaaa ttgatttaaa aaacactaga ctgtatctgc
    17941 tcagctttgt tggctactta aagtgggcag atacgtcatt agaagaaaaa acataagatg
    18001 tgaccactta cataaatttg tgctaaactg aggtagtaca agaataaatt acatcataaa
    18061 ggagtctttt taatgagtaa agaaatgaca gcgctaaaat tttattttag aaatggagaa
    18121 acctggacga tcaaccgccg tcatatcggc gatctttgga tcaaacaaat cacaacaagt
    18181 tttggacgaa tcaatggcag tgaatttgtt gaaatccatc cgtgtgctgg atttaaaatc
    18241 gaaatcttcc atgaagggga cgcagttgcg acacatgata tcaacctagg cggactggaa
    18301 atgggcatgt ttaatcgcgc attgaaatac gaagatatcg agcgaatgga aatcctttac
    18361 cgaaatggca cacctgatct tgtgtacttc ccatatctag ataaaggaac agaaggatta
    18421 gacaatcaat atcaatcaac aaaaatcagc gaaaaaacag gtaacttgta tatcgtgatc
    18481 aatccagatc aacgtgtaga agatgtatac ggggaatttt tcgaataaat cgatttttac
    18541 gcaaaaatga ggctgtgaaa gagatgtgtc ttattactac tgataggctg tctcttccac
    18601 accgtttatt cagaataagg gactgtgaca agatttttgt cgcagcccca tttttttgtt
    18661 gtgataaaag aacaagaaag gcaggaatgg gaaagtaaac atccttttgt cagaaacttt
    18721 tatctttctt aagttttact tgatatctga tactatttgg aatagaatga ggcgttgaaa
    18781 aatttatgga aatttctaac tagcaataaa cgatgaaaca atgaaatatt tttcatccaa
    18841 aaagaacttt gctataatga tggaagtttg tgtataggag atctggaaaa tgagagagag
    18901 ttttgaacaa caaaaaaaac tgttgcatga tcgctatggc gccctttcga tggatgaccg
    18961 ccgtcaaata ctttgtaagt tgcgtaaaag aaatatattg atgtatcgtc agttggaacg
    19021 tctgaaacat gatctgcttc gtttggagtc aaaacgtgta cagtgtgaat tagaggggaa
    19081 ccaaacacag gttgaagtag tcgaaaccaa aattttaaag aaaaaagaac aatttttaaa
    19141 aatgcttaca caaaataaaa aataaagggg atggacgatg gaatttgctg agttagttat
    19201 tgtatttgca gtaacgatca ccttatcgaa catagctagc cgcattttgc caatgattcc
    19261 agctccactg atccaaattt tccttggagt ggtattaggt ctgacagagt ggggacaatc
    19321 aatcgatttt gaacctgaat tgttcttagt tatgattatt gcaccattgt tatttagaga
    19381 aggagaaaaa gcagatattt cttcgattct caaaaatttc gggacaattt tgttcttggc
    19441 atttggagga gtcattttga cccttgtcgg ggtaggtgcg acgttatcat tcctgttgcc
    19501 aagtgtccca cttgctgcct gctttgcttt tggagcagcg ttaggtccga cagatgcggt
    19561 agcagtcagt tctctttctg gacgagtgaa tatccctaaa aaggcgatgc atattttgga
    19621 aggagaaggt cttttgaatg atgcatctgg tgttactgcc ttccagtttg ctcttggtgc
    19681 gctgatcacc ggatcttttt cagcagtaaa cgcggggatg agtctagtag tttctagtat
    19741 tgggggagct ttgatcggct ttttgcttgt ctggttcaaa caaaaaataa ttcatttgat
    19801 cgaaaaagca tcagcacaag atgtcacagg gtatctgctg atcgaacttt tgcttccttt
    19861 tctagcctat gtattggcag aggcatttga tgtatctgga atcattgctg cagtggcagc
    19921 aggtattctc caagcttctg gttttaggaa aatctctgtg tttgatgcag aattgtcgag
    19981 cttgtcccac agtacatgga caactattgc ttttacactg aatgcattag tatttatatt
    20041 tttaggaatc gaattgacgc aagtgttctc gccagtatgg ggagacggtt tatatccaaa
    20101 cggtctttta ttagcgatta tcgtattaat ctctgtcatg ctttttgtga tcagattcat
    20161 ttctatcagt cttttttatg tattcaaaga tggcagtaaa aagttcaaaa aacagttgaa
    20221 cgagatcttg atcttaacat ttggcggtgt aaagggaaca gtcagtttgg ctacgatttt
    20281 tattttgcca ttttcaatca ataatatgat gttttaccag cggtctttgc ttttgtttct
    20341 gacggcgggt gtcatcttag tgactttggt catagggatc attgtcttgc ctatgctgac
    20401 ggaaacagag gaagcgaaaa gcacggattt gaatgctttg atgattttag aagaggtcgt
    20461 tgaagttttg cgaaaagaaa taaaggaaat tgatcgtaat acaaaagaat ttttagccac
    20521 tgaagcagtg atagaaaatt atcaagaacg gatacgtgat ctgtacttgg aagatttaac
    20581 cgatgatgaa aagcaagaag tacaagaaat acaagcactg attttgtcga tcgaacggga
    20641 tggattggat gaaagttatc gttcgggcaa gctttcttct aatggttatc gtttttactc
    20701 ccgttttctt tctcgattcg aacattctat cacaagccag attttgtctt ttatcggctt
    20761 ttggttcatt gttgtccgta ggttaatgcg aatcgttctc catccgaaaa tgttttggca
    20821 aagaagatat gctgatcgtc agacatttat tagtgatgaa gatatccaag aaatcagaga
    20881 gacatatcaa aaaaatactc agttgattat tgaaagttta gataacttga ctgatattta
    20941 tgatgaaacg atggttcaat tttttatcca gcaacgcaaa cttgaaggaa taaaaatgaa
    21001 ctcggggaat ttgatttctt cgtggatgat ccagcaagat agtctgttta cgaaaaaaat
    21061 gctaagaggt tattatcttg aaagaaaaat cattgatgaa tacgaagttg ctgaaaaaat
    21121 cacgacgttt tctgcgaaca gctatcgcag gaatatcaat ttgttagaat cttatacgat
    21181 gaacaaacca tctgataact tttctttccg atttgcgttt cgagcaaagt caaaaaaaac
    21241 gagtccaata aaataaaaag gtaaaaattt tcaagtttta taaaaaacaa aacatatcag
    21301 aacaggatcg aatcggtcct gttctttttt tgagaacaca aaaagaacaa ataccaaaaa
    21361 aattttcctg aagttcacta tttttatgat acaatatcca agataaggag ggaacatgtt
    21421 gaaagaatta aaagtaactg acctcaaaaa aacttatggt gaaaaagatt tgtttgacca
    21481 gatttctttt ttggtccacg aaaaagaccg tatcgggttg attgggacga acgggacggg
    21541 taaaacaagt ctcttaaata ttattgccgg tatcgatagc agcgacggtg atcggcaaac
    21601 agtgttttat ccaactgatt accgtattgg ttatctttct caagcagctg aattttccga
    21661 tgaattaact gttctccaag cagtctttca aggaaacagc cccttgatcc aaactgttcg
    21721 agcatatgag gaagcattga tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa
    21781 acgttatgct aaagcagaag aacaaatgaa taaagaagat gcttggacta cagataccaa
    21841 cgcaaagatt attttgcaaa aattaggaat cagtgaatta gataaaaaaa tcagtacttt
    21901 atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg ttgattgatg aacccgattt
    21961 actattacta gacgaaccaa caaaccattt agattatgaa gcgatcgagt ggttggaaaa
    22021 ctatttgaaa caataccgtg gcgcactatt gatggttacc cacgatcgtt attttctaga
    22081 tagagtagca aaccgaatct tcgaactttc atttggaaaa ttatacgagt ataaaggaaa
    22141 ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga
    22201 aaagcgcaag cggctttata aacaggaatt agcttggatg agggctggcg tacaggcacg
    22261 tggcaccaag cagcaagcac ggatcaaccg tttcgaagat ctaaaggaga acttgtacca
    22321 agtaaaccaa gaagatgatt tagagctgaa cctagcgaca caacgtttag ggaaaaaagt
    22381 actggaaatc aaagatggca gttaccgtat aaatgaccaa acactgctag aacatttaga
    22441 tttattgatt caatctcggg aaagactagg tattactggt aaaaatggtg cgggaaaatc
    22501 gacattgctt aatattctag ctggcagaat tcccctggat agcggtacaa tgtctatcgg
    22561 tgaaacggtt catcttgcgt attatacaca agaaaatgaa gaaatggcac cagataaacg
    22621 gatgattgct tacttacaag aagctgctga agaagcaaaa acagcagatg gcagtcaaat
    22681 cggagtagca gaattacttg aacgattttt atttccaaga tttatgcatg ggacactgat
    22741 aggcaaactt tcaggcggag aaaaacgccg gttatttttg ttgaaactgc tgattcagca
    22801 acctaatgtt ttattattgg atgaaccgac caatgatttg gacatagcaa cattgacgat
    22861 tttggaagat tattttagaa gttttccagg tgctgtgatc actgtttccc atgaccgtta
    22921 tttcttggac aaggtagcag ataagttatt agtattccaa ggaaacggaa aacaagaact
    22981 ttattacgga aacatgtcca gttatttact gaagcaaaaa gagacccagc agcctgctga
    23041 aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa aagaagaaat tatcttacat
    23101 ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca gaacttgaag aaaaaatctc
    23161 attgctccaa gaagagatga atcatcaagg agataatttc actcgattac aagaattgca
    23221 aaacgacgta tctgaaacag aagcacaact tgaagaaaaa atggcacggt gggaatactt
    23281 gagtgaatgg gtagaagatt aggaggaaaa aggatggaac aagcgtatct tgacttagga
    23341 agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa caggcacagg cacattaagc
    23401 ttattcggat accaaatgcg gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa
    23461 cgagtacctt ttggattgat caaaagcgaa ttgctttggt ttttaaaagg agatacgaat
    23521 atccgctatc tattacaaaa taaaaaccat atttgggatg aatgggcatt tgaacgctac
    23581 atcaaaagtc cagactataa tggaccagat atgactgatt ttggccatag atgtttgaca
    23641 gatcctgaat tcaatgaaga atataaaaag cagagcaagc ttttttgcga gaagatccta
    23701 accgatgatt cctttgctga aacatacggc gatcttggac acatctacgg ctatcaatgg
    23761 cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca
    23821 atcaaaaaga ctcctgattc tagaagattg atcgtttctg catggaatcc ggaagatgtt
    23881 cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat tttatgttca agagggacgg
    23941 ctaagctgcc aattgtatca acgcagtggg gatgtgtttc taggcgttcc gttcaacatt
    24001 gcaagttatg cgttgctgac tcacctaatt gcacatgaaa caggtttagc ggttggcgaa
    24061 tttgttcaca cattaggaga tgctcatctt tatatcaatc atatcgaaca aatgaaagaa
    24121 caattgagcc gagaaatacg cgcatttccg actttagttt tgaatcagga aaagcagtca
    24181 gtatttgatt ttgagatgga agatatccaa ttagaaggat atgacccaca tccggcgatc
    24241 aaagctccta tcgctgttta gaatcagaaa ggatgagaaa atgttagctg caatttgggc
    24301 acaggatgaa aacggattga ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga
    24361 tctgaaattc ttcaaacaaa tgacagaagc aaatacacta gtgatgggac gaaaaacatt
    24421 cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg attgttttga cgagagattc
    24481 ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga
    24541 aaaagaaaca gacggtatct ttttcatcgc tggagggtca gctgtttatc aagaatttct
    24601 gccattttgt acgatattat accgtacagt catccatcat gtattcgatg gagatgctta
    24661 ttttccacct gttgactggt ctgattggtc actgatcaat actagtcaag gagaaatgga
    24721 tgaaaaaaat cgttatccgc atcaatttga aacgtatcag cgtaatgaga atcctgtaaa
    24781 aagcgggaca ggcgaattga cgatataaaa aagctatgga tagactagct gttatttaga
    24841 gctgtcccat ccatagcttt atttttttta ttaagtaaag aataaaacag agaagtacat
    24901 aaaacctgca cctaacataa caaacaaatg ccaaacaaca tgcatatagc ggatagaatt
    24961 caagctataa aagaatgcgc ctatcgtgta agaaagcccg cctgctacca tcaaaataac
    25021 accagtcatt ccaagagagt gataaagagg aacagcagcc acgacacaga gccaccccat
    25081 aaaaatatag atgatcgtag atacttttga tactctctcc cgtttatgca acgtcaacgc
    25141 tttatatata atgccaagaa tcgcaaacaa ccagattaat acaaataaag tccaacctag
    25201 ccatcctcgg atactcagca aacaaaaagg agtataactt cctgcaatca aaagaaaaat
    25261 cgaactatga tcgaatactt ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa
    25321 caaagtagac gttaaaaata aaagaatcat catcgatcca tagatagcat aagagacaat
    25381 atggagggag gaatgcaagt gggcaccttt tactaaaaga accactagtc ccgcgatact
    25441 caatccagtc ccaattccat gggtgactgc attaaacact tcatttaata tttcatacgt
    25501 tcgattaaat ttcgtttttt ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa
    25561 atcattattt gtctgttata ctaaaaatag aaattgtctt acatgtagtt taacatgaat
    25621 aaggcatctt gttaaagaaa gagagagaga aaataatgac aaatgtaaaa atcgtaacag
    25681 attcttcatg tacgatggaa aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc
    25741 tatccatcat gatcgacgga acagtatatc ccgatgatga ccaactagac ggcgaaagat
    25801 ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac aagccaacca ccaattggag
    25861 aattcatctc gatgtatgat gagctaggag aagacggcag tgaagtgatt tcgatccata
    25921 tgacaaaagg cttgagtgga acagtagaag cagcaagaca agccagtaat ctaacaaaaa
    25981 cgaaagtaac ggttatcgac agtgatacca ctgaccaagg attatctttt caggtaattc
    26041 gagcagctga attagcaaaa gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa
    26101 aagtacaaga caatacaaaa ttatacatcg gaatatccac actagataac ttggtaaaag
    26161 gtggaagaat cagccgaaca actggtttgc tgtctagtgt tttgaacata cgcgtggtga
    26221 tgaatttcgc ccattctgaa ttaatccctg taactaaggg gcgaggaaag aaaacgttcg
    26281 ataaatggtt taataatttg aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg
    26341 gtatttcaca tgcggatgca aaagaattgg cgcaagaatt caaagagggt ttgcaagaat
    26401 tattcccgga catgcatatc cctgtactgc atacgaatcc ggttatcgct acgcatactg
    26461 gtaaagacgc gtttgcaatc atgtattatg tagattagtc gaaaagagga taagccctca
    26521 gcctgaaagg cttgcagctt attctctttt tctttataaa attaggaaaa taattgtttt
    26581 cttttttcat aaagactacc tttttgataa aatttttgat aacattagaa ccaatcgaat
    26641 acaagaggtg aaaaaatgaa attaacgaaa cgacatttga tcctcgtttt gttcccatta
    26701 ttaattgcga tcatcatcta tgcaggttta gctgcttcta taccgaaagc tggcccacta
    26761 atgaaaccag taactactgt gagagaagat ccaaataaaa aagaaaaact tcactacata
    26821 gctatcggag attcgctgac tgaaggagta ggagatcaaa ccaagcaagg aggattcgtt
    26881 ccccttgtcg caaatgatct aaaagaccgt tataatttga ccgcggttga aacagaaaac
    26941 tatggtgtaa atggtgaacg aagtgaccag atcttaaagc gagtaaaaac aaaagaagaa
    27001 attcgaaaca atatcaaaac agctgatttt atcacactga ctgttggcgg taatgatttg
    27061 atgaaagtta tccaaaataa tttattcggg ttaacggtca attcctttaa aaagccactg
    27121 aaaaactatc aggaaaatgt cacagaacta ttggaagaat ttcgatccct caataaaacc
    27181 gcacctattt acattttagg catctacaat ccattttatt tgaactttcc tgagatcact
    27241 gacatgcaga caattgttaa caattggaac aaaggaacag aaacggtgac aaaaaaaatg
    27301 ccgaactgct actttatccc aatcaatgat ttgctttatc aaggattgaa tcaggaagta
    27361 ggtgtcacag aagaaggaac agatcaaaca actagcacga cagaaaatct aaatggcatc
    27421 aaaaataatg tgctctatga agaagatcat ttccatccaa ataatttagg ttatcaattg
    27481 atggcaaatg cagtgagaga tcagctgatc gagacacaaa accaatggtt agagaaggag
    27541 gatgatcaat aaacatgaaa acaagatctg acgtatcttc aaacaaaaca aagaaaacaa
    27601 attactggaa attcgccttc cttatccttc ttggggtagt acttggttcg accgtatttt
    27661 taggcagccg tatttttgct aatcgggaac cagaactccc agaaatacca gctcttacgg
    27721 aaagacaagg agatcctgtc ttaacgatca attcaaataa agaaaaagtc aatcaaatca
    27781 tcagcttttt ccttagtgag tatcaaaaag atagtgatat tgaatataaa ttttatttgg
    27841 aaaatgaagc actactcaat ggtactttcg aagtactagg ttttcctatc gatttctatt
    27901 tatattttga tccgtatgtg atggaaaacg gaaatgtcca actaaaagct agaagtcttt
    27961 ctatcggaac attgagtttg ccaatcaaag atgtgatgaa tatgatcaag agaaactata
    28021 aattaccaga ttggatcgaa gtcaatacag atgatctgac tatcatgctt aggttggatc
    28081 aattccgaat gcaaaacggg atgtatatta aagcagataa aatcgactta gtaaatgatg
    28141 acattcggtt tagtctatat ttgccaaaag atacctctga tgcttcaaac aactcaaata
    28201 ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa agaaaataaa aaatcatcgg
    28261 atgagtagta ttagaggggg aatcataatg gaacgagcaa tatttgcagg aggctgcttc
    28321 tggtgtatga tccagccttt cgacacgctt cctggtatcc atacagtgat gtcaggatat
    28381 actggagggc atgtaccaga tccgacttat gaacaagtaa aaagccaaac gactggacat
    28441 acagaagcag tggagattat ctatgatcca acattgattt catatgaaca attagtagaa
    28501 ctttactggc agcaaactga tccaactgac gctttcggac aattcgagga tcgcggtgac
    28561 aattatcgcc cggtgatctt ttatttgaca gaagaacagc ggatcattgc tgaaaaaagt
    28621 aagaaacagt tagctgaaag tggacgtttc gaacagccaa tcgttacaaa aatcgaacca
    28681 gcccaaccat tttatctagc agaagaagac catcaagagt attataaaaa gcatcctgaa
    28741 aattttaaaa gaaaccatgc acgccgggct gcctttatcg aagaaaattg gaggaacgaa
    28801 gggtgaatcg aagctttttt cattatttaa tgacactgcg aggcggtggc acacatgatg
    28861 ctttatctgt tttcgctaca gaagccggta aagacatcca atttcctaag cacagctcta
    28921 gctacgaaga aatttctaat tatctggaac tgaatgtcga ctatttgccg tccatggata
    28981 tctttgacga agcatgggaa aaatatttag aaaataatca tgacatataa gagatagttt
    29041 tgtactttat gatcaaaaaa gttttttgtc caatagccta attgaaagaa ttatttttat
    29101 ttcagctatc tagtcgcatt tttttgatat ttttcttata ataaagaaag aatgaaaaaa
    29161 agagggatgg attgatagag attgacaaaa aaacaacgct atattaatcg cttctggctt
    29221 gtattcaaat acttacttgt ttctgtattc cttgcgttca tgttgcgggg atttttattc
    29281 atcccggtac cagtggaagg caattcaatg gaaaacgtgt tgaagcaagg tgatatggtt
    29341 gtgatggaaa aattttcgga gatcagacga tttgatattg ttgtttttca attagcagat
    29401 ggcacgattt atatcaaacg agtaatcggt cttccaggag aaaatgtcag ctaccaaaat
    29461 gatcaattga agatcaatgg gaaagtagtt aaggaacctt atctaactaa aaacctgaaa
    29521 tcagatcatg caaatgcatc ctatacgacg gactttacac tacaagagct aacgggacaa
    29581 tccaagcttc cggaagataa ttattttgta cttggagata atcgaagagt ttcaaaagat
    29641 agtcgttcgt ttggcacgat caataaaact gatattctag gtaaagcaag atttgtctat
    29701 tatccacttg atgaaataaa atggatccag tgagaaacaa taaagcgtgc agtctgttga
    29761 gactgcacgc tttattgttt aatccaaaat cagtttacta ccttagtacg taaaatcaaa
    29821 cagctcgttt aaacactttt ctagcctcta aagttttgat aaatgaataa acaaccatta
    29881 tttgaatagg gatttggatc atattcctga tgaatctacc tagaaaaatt actttgaaat
    29941 ctgcaccaaa caaaacagac atcagccaag aattgataaa aaggctgact gggatcataa
    30001 tcaataattg ggcagcgatg acggaaggca ggtttatctt tttatgatat aaaaataagc
    30061 cattaataaa tccaccaagg aaagccgtga taagaaaagc agcttgaaac gttcctgaac
    30121 caaatatcgt gaaatttaaa gtgttcgaca acatttcaag caaaccagcc cagatcggtc
    30181 catatagata acccataagc gccatactta aaaatccgaa tgtgatttga aaataagcag
    30241 gaccaatcgc tatcttgtat aagatgacat caagggctaa taaaagagca gaggtcgaca
    30301 acaatcttgc attcatttcg ggcaatctaa aaacgtcttt tgcaatcaaa gtgttcaaaa
    30361 tattctctcc tttgtcagtt aataataaaa ggataccata aaatcaccaa tgtgtcatga
    30421 cacattggtg atttacatac actggcatct aacaattaaa aaaagggtga gaagactgct
    30481 tttataaact gtaaaaaaga gattgtacga ataaaaaaaa tagaggcgga gaatacttat
    30541 cgtatcatcc gcctttgttt ttttgttata aaaaacgtca gattagttat ctgcttacat
    30601 cttcatccat acgggttcta atgtatttcc gtctagatct tggacttcta atgaaaacat
    30661 ttcttcttca ggcattccca tatctacttc gtggaaagta ccaccatttt cttttgcaat
    30721 ttgagcaaat ttttttactt catccgcatt ttcaacacta aacgaaatca aagcaccact
    30781 tgttttttta ttatcagcta tttctttatc ttttataaat tttttataaa aatcatgaga
    30841 gagaagcatt atccaaaaag tgtcatccca taccattgaa ccagcttctt cagtagaaaa
    30901 ttcttcattt tttttaaaac ccagcttttc ataaaaagca acggaacgat tgacatcagc
    30961 aacaggaaaa ttaacaaaaa ccatactatt catttgtttc gtctcctttc tgttttgttc
    31021 gtctatcatc tcttatttag gaaacgctgt caaaaattta gattcatata tagaaaccaa
    31081 aataaaaatt tgcatcatca aaaaagtgaa gatgtgatag aatgaggact ggttcacttt
    31141 cacttaacta atagatggaa tttctttata ataaaaaagt gaatttatat tgaaaaagaa
    31201 aaaaacaagt aaagccgggg aatattccgg tatgaaagga atggagagaa caatgaccat
    31261 ccaatggttt ccgggacata tggcaaaagc ccgacgagaa gtttctgaaa aaatgaaata
    31321 tgtggacatc gtatttgaat tggtagacgc acgattacct ttatcttctc gtaatccaat
    31381 gctagatcag atcattcagc aaaaacctcg ccttgtttta ttgaataaag cggatctggc
    31441 agatccacaa cagacaaagc tgtggcaaga atattttcgt aaacaaggcc atcttgcatt
    31501 agctatcacg gcacaagaaa gtaaaggaat taaaggtcta gtacctgcgg ctaaagaagc
    31561 attgaaagaa aaacgggcaa gagatgctgc tcgggggatc aagcctcgcg ctattcgggc
    31621 aatggtctta ggtattccaa atgttgggaa atctacgttg atgaatcgac tagtaggcaa
    31681 gaaaattgca cagacaggaa ataagcctgg cgtaacaaaa ggtcagcagt ggcttcgttt
    31741 aggtaatgag ttggaattgt tagacacacc agggatttta tggcccaaat ttgaagatga
    31801 agaaatcggc aaaaaactag cgctgacagg cgcgatcaaa gaccagcttg tccatttaga
    31861 tgatttagcg atttacggat tagacttttt tgcccgatat tattcaggaa gaatcagcga
    31921 tcgttatggt ttatcaatcg agcaagaaga aaaattggca ccggaattac tgatggatat
    31981 tacagcaaaa cgtgggtata aagaagacta tgatcgtggc agcgaattag tcgtgcatga
    32041 tattcgtcaa ggaaaacttg gacgatacac actggatcgt tgcgaagaaa tggagcaaaa
    32101 aagtgatgac tgaatccatc caaagtatca aagaacgatt gaaaagcata cagagaatag
    32161 atgatcctac actagaatca ttaagaaatg atccacgcaa aggcgtgcag caagcactgg
    32221 cttcttttga acgacgattg aaaaaggaag cattaattaa ggaaaaatat gaagaaatgt
    32281 tttgctttga aaagcaaggg tacgcacaag gtcaccaatg gattgccggc attgatgaag
    32341 taggaagagg accattagct ggacctgtcg tggcagctgc agttatcttg ccgaaaaatg
    32401 agatcatatt agggctgaat gactccaaac aattatcaga gaaaaagcga gaacttcttt
    32461 atcaacaagt tcaagaaaaa gccctggcaa tcgggatcgg gatcgttgat cagcagacaa
    32521 tcgatgatgt gaatatttat gaagcaagta aattagcgat gatccaagca gtagaaaaat
    32581 tgtccattgt accaagctat ttgttgatcg atgcgatgac cttggatttg cctattcagc
    32641 aggaaaaaat aatcaaaggg gatgcacgat ctgtgtcgat tgctgctgca agtatcattg
    32701 ctaaagtata tcgagaccaa ttgatgaaag aatatgacgt gctttatcct ggatatggtt
    32761 tctcgcataa tgcaggttat ggaacaaagg aacacttaga aggacttgaa cgacaaggaa
    32821 ttacgccaat ccatcgtaaa agttttgcac ctgtaaaaaa atatttacaa taaaaaacag
    32881 ccgcttttac gtatcttatt agaaagagag acgaaaggcg gttttatgat gtatcaaata
    32941 gaagaaaatt tattgaaact tgcttataca aaaggaatca gtagtcaagg aaaatgggca
    33001 cttgctaact ggatgatgca gtatccttat aaagaaatcg gatttgaaga catcattaag
    33061 ataggtaaaa taaccaaaca ccgggagctg tttcgcaaaa gttggcagga aattcacgct
    33121 aactggggca aaatccaatc gaaacagtcg tttatcactt gctttgatcc caaatatccc
    33181 cctcagctgc ttcacttgac ctatccgcca attgtactgt tttataatgg tgatctatct
    33241 ttactttctt gtaacatgtt aagcgttgtt ggtggaagac acacttctgc tttagccaaa
    33301 aaaacagttt attatttact aaaaccagtg atagaagctg gatatgtgat cgtcagtggc
    33361 tgcgcgaaag ggatagatac atacggtcac caagcagcaa tctctcatgg tggacggaca
    33421 atcgcagtca ttggaacagg gatagagcaa gtctatcctg ctgaaaatcg tgacttgcaa
    33481 gaggtgattg caaatgatca tcttcttttg agtgaatatc ctcctcaatc cggcccgaaa
    33541 aagcattgtt ttccaatgag aaatcggatt atcgcagctt tgtctaaagg aacatgtgtc
    33601 tttgaagcca agaaaaaaag cggctctctg ataacagcac agcaagcatt ggatcttgga
    33661 agaacagttt tttctgttcc aggaaatatt ttaacagaac attctaccgg atgtcatcat
    33721 ttgatccaag acggtgcagt ttgcacggtt tcgggtgaag atatcttggc agaatttaaa
    33781 gaataacacc atacttttca tttgcttttc ttgacaaacg ttttctgaat ggatatgatt
    33841 agctacgatt tgtatgaatc ctcttttaat agtaggaaca cgacaatgga aaggagtcca
    33901 ttcagtaatg gcgtataaat atttagtgat tgtagaatca cctgcaaaag ccaagacaat
    33961 tgagaaatat cttggacgaa actataaagt aatggccagt gttggccata taagagattt
    34021 accgaaaagt aaaatgggta tcgattttga aaacaattat gaaccccatt atatttctat
    34081 acgcggaaaa ggcgatgtca tcaaaagcct gaaagccgca gcaaaaaaag ctcaaaaagt
    34141 ttacttggca agtgacccgg atagagaagg agaagcgatt gcttggcatt tagcgtacct
    34201 acttgggttg gatctgaaag aaaaaaatcg ggtggtcttc aatgaaatca caaaagacgc
    34261 agtcaaagca gcttttaagg aaccaagaac gatcgatgta gatttagtag atgcacagca
    34321 agctcgtcgt accttagaca gaatcgttgg ttattcgatc agtcctattc tctggcgtaa
    34381 ggtcaagaaa gggttaagtg caggacgtgt ccaatctgtc gctttaaaaa ttattattga
    34441 ccgtgaaaaa gagatccgag aatttgttcc agaagaatat tggagcatcg acggtaattt
    34501 taaaaaagct cgcaagaaat tcaaagcaaa tttctgggga atcgacggta agaaaaagaa
    34561 attaccagat gcacaaagtg taaaagaagt cactgctaga attgatggaa cagacttcga
    34621 agttaaaaaa gtagaaaaga aagagcgcaa aagaaaccct gcgaatcctt tcactacaag
    34681 tagcctgcaa caagaagctg cccgtaagtt aaactttcga acaagaaaaa ccatgatggt
    34741 agcacagcaa ttatatgaag gaatcacttt gggtaaacaa ggaacagtcg gcttgattac
    34801 atacatgcgt actgattcta cccggattgc cgatacagca aaagcagagg ccgcagcatt
    34861 tattgaagat acgtatggaa aagaattttc tagacatgat caccgaaaga tcaaaaatgc
    34921 tcaaggcgca caagatgccc atgaagctgt gcgtccaacg agtgtgcttc gtacaccaga
    34981 tgaactcaaa caatatttag ataaagatca attgaaactt tatacattga tctggtcacg
    35041 ttttgtggct agtcagatga caccagctat tttagataca atgaaagtga cattagaaca
    35101 aaatggtgtc atcttcattg cgaacggttc aaaaatcaaa ttcaaaggat tcatgcaagt
    35161 gtatgtggaa ggtagagatg acggcaaaga agagaaagaa aatattcttc cagaacttga
    35221 agaaggcgat gtagtccaat ctgttgatat tgaaccaaaa caacatttta ctcaaccacc
    35281 agcacgtttt agtgaggcta cgttgattcg tgcactagaa gaaaacgggg tagggcgacc
    35341 ttccacttat gcaccaacac ttgatacgat ccaaagacgc tactatgtaa aattgacaca
    35401 aaaaagattt gagccgactg aactaggaga aattgtcaat tcattaatct gtgaattctt
    35461 ccctcaaatc gtcgatatcc atttcacagc agaaatggaa ggagatttgg ataaaattga
    35521 agaaggcacg gaagcgtggg tcaaagtggt tgatcgtttc tataaaccgt ttgaaaaaga
    35581 actgacgaat gcggaagaaa aaatcgaaaa aattcaaatc aaagatgaac cagcaggatt
    35641 cgattgtgat gtctgtggac atccgatggt gataaaatta gggaaatatg ggaagtttta
    35701 tgcttgcagt aattttcctg attgtcggaa tacgaaacca atcgtaaaag aaatcggtgt
    35761 gacttgccca gtatgtcatg aaggacaagt cattgaacga aaatctaaga aaaacagact
    35821 attctatggt tgttcccgtt tcccagaatg tgaattcact tcttgggata aaccagtggg
    35881 cagaaattgt ccaaaatgtg acggatatct ggtcgagaag aaagtcaaag gcggtaaaca
    35941 agtagtctgt gtaaatggag attacgaaga agacatccaa aaataataga aaagaggttc
    36001 tttcattgaa aatggtaaat gttgttggag cagggcttgc cggaagtgaa gctgcttggc
    36061 agatcgcaca agcaggagtc cctgtccgtt tatatgaaat gcgtccagtc aaatcgactg
    36121 aggcgcatca cacaagtaat tttgctgaac tagtatgttc caattcatta agaggaaaca
    36181 gtttggctaa tgcagtaggg ttattgaaag aagaaatgcg ccgattgaat tcggtagtaa
    36241 tcacttcggc agatgaaaca gcagtgccag ctggtggtgc cttagctgtt gaccgcgatt
    36301 ctttttctga aacaattacc cgaaaagtca aagagcatcc attagtaaca gtgataaacg
    36361 aagaaattac tgaaataccg gaaggaatca ccgtagtagc aacaggacct ttgacttctc
    36421 atccattagc agaaagcatc aaagcattca atggttcaga tggtttttat ttctatgatg
    36481 cagctgcacc aatcgtagac aaagctacga tcgatatgga taaagtatat ttgaagtccc
    36541 gctatgacaa aggagaagca gcttacttga actgtccgat gacagaagag gaattcaaag
    36601 ctttccatga ggcactaatc aatgccgaag tggcagaact taaatctttt gagaaagaaa
    36661 aatattttga aggatgtatg ccaattgaag taatggcaca gcgaggattc aaaacgatgc
    36721 ttttcggacc tatgaaacca gttggattgg aagatcctaa aactggcaaa cgtccttatg
    36781 ctgttatcca gctgcgtcaa gacaatgctg cagcttcttt atacaacatc gtgggctttc
    36841 agactcactt gaaatggggc gagcaaaaac gtgttttccg gatgattcct gggttagaaa
    36901 atgctgaatt cgttcgctat ggtgtgatgc accgaaacag ctttatgaat tcgccagaat
    36961 tgctgaaacc gacttaccaa tcaacgaaac gcgaagatct gttctttgca ggtcagatga
    37021 ctggtgtaga aggctatgtt gaaagtgcag cgagcggact acttgctgga atcaacgccg
    37081 cacgcttagc aaaagaaatg gaaccagtcg agtttccaca agaaacagct attggaagta
    37141 tggcttacta cattactcat gcggaaggaa aacatttcca accgatgaat gccaactatg
    37201 gtctgtttcc tgaactgcca gagcgcattc gagacaaaaa agcaagatat gaagcaattg
    37261 cacaacgagc tttaaaagct aatgaggcaa taaaaacaga attagaaaaa actgcaatcc
    37321 caaatgagta aggaaactcc atgaagaggg tgagacggat agtctcgctc tttttcttgc
    37381 gtttttctta agaatttctc aaacaaaatg tgaggaattg cgccaattgt gaacaaactt
    37441 agaaatattg gttaaattct gacaatttat ttgtcttttt ataaacgctg tgctaaatta
    37501 ttctctggga caaaaggaag gtgagaaaat gacagaagcc acgtgggaac aaaaattttt
    37561 gcgttatttg attgtcgaaa gaggatattc agaaaaaacg aaagaagcat atgaagaaga
    37621 cattctccat ttcaaacatt ttttggagga atctggagac gctgatctgc tgaaggcgga
    37681 atattttacg gttcgtgtct atttgagtgc attagctgat catcgtccta gctatagcag
    37741 aaattctatc agtcgcaaga tagccagcct acgttcgttt tatcagtatc ttctcaaaga
    37801 agaagtgata aaagaaaatc cattttctta cgttcatctc aaaaagaaaa atttgagact
    37861 gccacgtttt ttttatgaaa atgaaatgca agcacttttt gacagcgtag caggagatag
    37921 tcctttggat ttgaggaacc gcgctttact ggaagtcctt tacgggtcag gcatccgttt
    37981 aagtgagtgt agcagtttgt cggtatcaga cattgatttt gattcagagg tcatgttgat
    38041 ccatggtaaa gggaacaaag agagatatgc tcctctaggt tcttttgctc aagatgctct
    38101 gcaagaatat ttcagggaag gccgccaggt attgatgaca aaatatcacg aagagcatga
    38161 tatcgttttt gtgaatcatc atggaaaacc aatcacgcct actggaattg aatatgtctt
    38221 gaatcaaatg atcaaaaaaa gcagtttgga tagtgatatc catcctcaca tgctgcgcca
    38281 tacatttgct actcatttat taaataatgg ggcagatttg cggacagtcc aagaattgct
    38341 aggacatgcc aatttatcga ctacccagat ctatgcccat gtaacaaaag aaagtttaca
    38401 aaaaaactat cgttctttcc atccaagagc gtaaaaataa ggaaaagaac aaatttagga
    38461 ggaaacaaac atggtcgaat caccatttca ttcaaccact atctgtgccg tcgaaaaaga
    38521 cgggaaattt gctatggctg gagatggcca agtaacaatg ggtgaatctg ttgtcatgaa
    38581 aggcactgca aagaaagtac gacgtatcta caatggagaa gttgttgtag gtttcgccgg
    38641 aagtgttgca gatgcgttta ctttagaaga aaaatttgaa ggcaaactaa acgaatataa
    38701 cgggaatcta caacgcgcag ctgtcgaatt agctcaagaa tggcggacac agcaatctat
    38761 gcaaaaactt gaagcgatgc tgatcgtaat gaatgataaa gaaatgcttc ttgtttcagg
    38821 tacaggagaa gttatcacac cagatgatgg tatcttagct atcggatcag gagggaattt
    38881 tgcgttggct gcagcccgag caatgaaaaa ctatgctcaa aacgacatga gtgcgaaaga
    38941 aattgcagaa aatgcgttaa atattgccgc agatatctgt atctttacta atcataatat
    39001 tatcgtagaa gaactgtaag aggtgagtag atatgtatga attaaaacaa acaccaagac
    39061 aaatcgtaag tgaattagat gaatatatcg taggacagca cgcagcaaaa aaatctgttg
    39121 ccgttgcttt gcgtaaccgc tatcgccgtt tgcagttaga tgaaaaaatg caacaagata
    39181 ttacaccaaa aaatatgctg atgatcggac ctacaggggt cggtaaaaca gaaatcgcac
    39241 gacgtttggc aaaaatcgtc aatgcaccat ttgtcaaagt cgaagcaaca aaatttactg
    39301 aagtcggtta tgtcggaaga gatgtagaat caatggttcg ggatttagta gaaaatgcga
    39361 tccaaatcgt agaaaaagag cagtacagcc gtgtatacac aagtgctaaa aaaaatgcag
    39421 aaaaacgttt ggtaaaaatc ttggtacctg gtatcaaaaa agaacagaaa caatccacta
    39481 atcaatttga acaaatgatg aatcttttta atgctagtca gcaaaatacc gctgaaccgc
    39541 aagaagaagt gacagaagat attcgaatca atcgccgtac agtcttggaa caactacaaa
    39601 agggtttact ggatagtcgt gaagtaacca ttgaagttga agaccaaaag aaacctgcac
    39661 caatgatgaa caatggattg gagcaaatgg ggatcgacct aaatgaaact ctaggtgctt
    39721 tgacaccgaa gaaaaaagtg gaacggacgg taacagtcaa agaagcttta gagttattga
    39781 tcaaggaaga atcagcaaaa ttagtcaagg atgctgatat ccatagtgag gcaatcaaat
    39841 tagcggaaga aagtggaatt atttttatcg atgaaatcga taagatcact tctaaatcac
    39901 agcaatctgg agaagtatcc agagaaggcg tccaaagaga catattgcca attgtcgaag
    39961 gttcacaagt aaacacaaaa tacgggatca tccaaacaga tcacgtccta ttcattgctt
    40021 caggagcttt ccatttgtct aaaccaagtg atctaattcc agaacttcaa ggacgcttcc
    40081 cgatacgggt agaattagat gatttgacag ctgaagattt tgtgaagatt ctgaccgaac
    40141 caaataatgc tttagtaaaa caatacgtcg cgttgatcgg tacagaaaat gtgtcagtta
    40201 cgtttacaaa agaagcaatt gaacggatcg ctcaaatcgc tttcgatgtc aaccgtgaca
    40261 cagataatat cggcgcacgc agactgcata ccattttgga aaaactacta gaagatctat
    40321 tatttgaagc accagatatg caaatgggag aaataacgat cacagaagca tatgtggatg
    40381 acaaacttgg ctcaattgcg gcaaatgaag atttaagtcg ctatatttta taaaaaatcg
    40441 gaggacgaca atgacagact tgctaacgaa aacccgcaag ataaacacac ttttgcagca
    40501 aaagaatact tttgacatgc aagcagattt gccttacgat aaaatggctg tcacactagg
    40561 tggtattctt gacagcaaca cctatatcat aggaagtgac ggagtattac taggttataa
    40621 tgaacgtcac gatgtgaaca atgaacgaat caaaacaatg tttgttgaga aacaatttcc
    40681 tgaatcttat actgatacag tggcacagtt gatgaaaact gaagctaata tcccaattac
    40741 tagtgatatt actgcatttc cggtagagtc aagagagaaa tatccatttg gtctgacgac
    40801 tgtcgttccg atctttggag ctggcgaacg tttgggtacg attatcttat ctcgtatgga
    40861 acaatcgttt gcagatgacg atctagtctt agcagaatat ggagctacgg ttgtcggtat
    40921 gcagatcctt tatcagaagt ctcgaagcat cgaagcagat gtacggagtg caacggctgt
    40981 ccaaatggca atcaacacat tatcttatag cgaattgaag gctgtacaag ctatctttga
    41041 agcattggat ggagatgaag gtcgtctgac tgcttctaat attgccgata gtatcggcat
    41101 cactcgttct gtgatcgtca atgctttacg aaaattggaa tcagctggaa tcattgaatc
    41161 tcgttcgtta ggcatgaagg gaacttattt aaaagtattg aattcccgtt ttaaagacga
    41221 gttagcaaaa cacagttatt agaagtaagg ggactggggc agatagaaat gtttgttcca
    41281 gccttcccat tttaaaaagt aagtcgaagt tggaggaatg gcaatgactg ttattattca
    41341 aaatgaccag ctgatcgccg aaatcgctga acacggtgcg gaattgatca gtctgaaatc
    41401 aaaagaacat ggtttagagt atatctggca aggtgatcca gcgtattggg gcagacatgc
    41461 accggtttta ttcccttttg ttggacgttt aaaagatgat cagtatgttt atcaagggaa
    41521 aacatatcaa atggggcaac acggatttgc tcgggatatg gattttgaag tcattgaaca
    41581 tggacaagaa atggctagtt tcatcttaag gagtacgccg gagacattag aaaagtatcc
    41641 atttgatttt gaattagtga tcagctatga attaggagga gacggcatca cggttcatta
    41701 tcaagtggca aataccggtg aaaaagaaat gttcttttct atcggtggac atcctgcttt
    41761 caatgtacct cttgaagaag gattgtcatt tgaagatttc tatctggcat tttctccatt
    41821 gaaatcaaga ttacgcctac cattgaaagg cccattcatc gatatggatc aaaagacgtt
    41881 aggtcagaca aatacgaatc tggctttgaa tcgtgaactt tttgagcaag atgccttggt
    41941 ttttgaaaca aggggtctaa atgcgtacgc cattcgaagt gaaaaatctt cccatagcgt
    42001 aactttaagc tataaagata tgccttatgt agggatctgg tctccttatc caaaagaagc
    42061 gcctttcgtt tgtatcgaac cgtggtgcgg aatcgctgac acggtgaaca gtagtggaaa
    42121 cttgatggaa aaagtaggga tcaatcaact aggtgcccat gaaatcttca agacaaaata
    42181 ttccatcaca gtgaagtaag aggttgtgac cgagaagtta gcagggaaca ccgtttacca
    42241 aggtgtgtaa aaaagctatc ctgtaagtaa taagggagtg tgacatcgat ttgtcacact
    42301 cccgttttat ctctttggaa tgaaaaagta tcaggatttt ttcttgctgc tattcaaacc
    42361 aaacgggatt cgactttcag tatggtttct gatacgcttg atattgtctt tatgccgata
    42421 aaagataaag gaagaaacta aaaaggcaat gatcgtcagt aaccaattgg gcttagccaa
    42481 aatcacagga gctacataag gaaggacgat cgtagataaa gtgatcaata ctgcactgat
    42541 catactgctc aaactcacca tacttgtaca aaataaacta acgataaaga tgatacaaga
    42601 ataaacaaaa aatacaggat taaatcctaa aagcattcct gcacttgtcg caacagcttt
    42661 tcctcctttg aacccagcaa aaatcggaaa tgtatggcca agaatggcgc ataccccaaa
    42721 ccaaagaggg ttaatcccag aaaggtggaa gatcatcggc aaacaagtag ccaatgtacc
    42781 ttttaaaata tccataagca ggacaacaat cccggctgtt ttgcctaata cacgaaatgt
    42841 attggtcgtt ccagtattac cgctcccgtg ttgtcggata tcttttttga aaaacaattt
    42901 gccaatccag acaccggaag gaatagaacc taaaagatat gctaaaataa gtaataagat
    42961 caatttcatt ttttaccctc tttcaagttg aaaactatcc tttattttag catgaatagg
    43021 taacaggaac aattgaaaaa atgaaagata caagtataat agatggaata aaaagaaaag
    43081 caaggggtgg cagaaatgtc tgtaaaaaat ccaacacaag aaacgatttt caactatttg
    43141 gaagaagcaa aaaatattgc cgtcgtaggc ttgagcaata aaccagaacg aacaagttac
    43201 atggttgcac aggtattgca agacaatgga tataaaatca taccagtaaa tccattattg
    43261 gctggtgaag aaattctagg cgagaaagtc tatgcgaatt tgacagaaat cgatgatcca
    43321 atcgatatcg tagatgtatt tagacggagt gaatttttac ctgaaatagc aggtgatttt
    43381 ttgaaaactg atgctaaagt gttctgggca caattaggga ttgaaaacaa agaagcagct
    43441 gagatgctaa gaaaagcagg acgtacagat atcgtaatgg atcgttgtat taagattgaa
    43501 cttggtaagt tgaaagaaaa ccaaaaataa aaatgaccac aaagaagaga tgatcgatag
    43561 aggggaatgt cgaaaaagcg gttaggagat aattaaaatt gatttctcgt ctaccctctt
    43621 ttttttcacg accttttcta gtatgataat cgtggtgcgg ttttgccgca aagtattatt
    43681 gaaggagcgt ttgccttggc aaaaaaagta aacaacgaat acaatgactc gtccatccaa
    43741 gtattggaag gattagaggc agttagaaaa agaccaggga tgtacattgg atccactgac
    43801 agccgaggct tgcatcattt agtctatgaa atcgtcgata atgcagttga cgaagcatta
    43861 tcaggttatg gcagtgagat tgatgtgacc atccatgaag acaatagtat aactgttgca
    43921 gatagcggac gtgggatgcc tgtaggaatg catgcttccg gaataccaac agttgaagtg
    43981 atttttactg tgctgcatgc tggcggaaag tttggtcaag gagggtacaa aacttctggt
    44041 ggactgcacg gtgtaggagc cagtgtggta aatgccttat cgaaatggct aattgtaacg
    44101 atcgttagag atggcgtaga gtatcagcag aaatttaaaa acggcggtaa acctgacggt
    44161 actttgaaaa agataggaaa aacaaaaaaa gcaaacggga cgactgtcca ttttttacca
    44221 gatgatacaa tattcagtac aacgaaattt tcatatgaaa ttttagccga acggttaaga
    44281 gaatctgcct ttcttctaaa aggcgttaaa atctctcttt ctgatcttcg tggagaagaa
    44341 ccagtcaagg aaatttttca ttacgaagaa ggaatcaaag aatttgttga ttatctgaat
    44401 gaagaaaaag atacattgac accagttgtt tatttttcag gagaaaaaga aggtatcgaa
    44461 gtagaggtcg cttatcaata caatgacggt tattcagaaa atgttttatc ttttgtcaat
    44521 aacgttcgga caaaagatgg gggaacccat gaagctggta tgaaagcggc gatgacgaaa
    44581 tcttataatg aatatgcgag aaaagttggt ttgctgaaag aacgggacaa gaatctggaa
    44641 ggtagtgatt tcagagaagg gttagctgct gtactttcta tccgtgtacc tgaaaatctt
    44701 ctgcaatttg aagggcaaac aaaagaaaaa cttggtacgc cagttgctcg taccgtagta
    44761 gataacgtca tcagtgaaca aatgggcttt tatttgcagg aaaatagcga aatgagccag
    44821 atgttggtcc gtaaagcaat caaagccaga gaagcgagag aagcagcacg taaagccaga
    44881 gaagaaagcc gaaatgggaa gaagcgtaaa aaaggcgaat ctcttctttc aggaaaattg
    44941 acacccgcac aatcccgaaa ccctaagaaa aatgaattgt atttagtcga aggggattct
    45001 gctggcggat ctgctaaaca aggaagagac agaaagttcc aagcaattct acctttacga
    45061 ggaaaagtca tcaataccga aaaagctaaa atgcaagata tcttgaaaaa tgaagaaatc
    45121 aatacgatga tctatacgat tggtgcagga gtcggtcctg agttttctat cgaagactgt
    45181 aattacgaca aagtaatcat catgaccgat gcggatacag acggcgccca tattcaagta
    45241 ttactgctta ctttctttta tcgttatatg aaaccgctga ttgaagctgg gaaagtatat
    45301 atcgcgttac cgcctttata taaagtatcc aaaggacaag gaaagaagca agtcattgaa
    45361 tatgcgtgga cagatgacga attggcagca atgattaaaa aagtcggcaa aggatatatg
    45421 ctgcagcgat ataaaggact tggagagatg aatgcggaac aattgtggga aacaacgatg
    45481 gatccgactt cccgtacgtt gatccgcgtc cgtatcgatg atgctgcgca agctgaacgt
    45541 cgagtaacca cactgatggg tgacaaagta gaacctcgaa gaaaatggat cgaaaatcac
    45601 gtgcaattta cgctcgaaga agatggcagt atcttagata aaaaagagga tactgaaatc
    45661 tctccttcag tttctaatga tttattagat gaagaacgag cagataaaaa tgaaaacaat
    45721 cagttatttg aagtagaata ggaggtcacc tgatggaaaa tcgacaagaa gtacaagaat
    45781 taactttaga agaagtgatg ggtgaccgct tcggacgtta ttccaaatat attatccaag
    45841 aacgtgcact gcctgatatc cgtgatggat tgaagccagt gcaacgccgg atattatttg
    45901 ccatgaacaa agatggcaat acgtatgaca aaggattccg taaatcagca aaatctgtcg
    45961 gaaatatcat ggggaattat catcctcatg gtgacatcag tatttatgaa gcgatggtcc
    46021 gcatgagcca agactggaag ttgcgggaag tactgatcga aatgcacggg aataacggga
    46081 gtatggacgg tgatccacct gctgcgatgc gttatactga agccagatta tccaagttaa
    46141 gtggagagat gctagcagat atcgagaaag aaacagtcga tctcgtgtgg aattttgatg
    46201 atacagaaaa agaaccaaca gtccttccag ctcgataccc aaaccttctc gtcaatggat
    46261 cgacaggtat atctgctgga tatgcaacag aaatcccgac tcataactta gctgaagtga
    46321 tcgatggcac aatctatatg atcgatcatc cacaagcgag ccttgaaaaa ttgatggaat
    46381 atattcctgg acctgacttt ccaacaggag ggatcttgca aggaaaagat gaaatcaaaa
    46441 aagcttatga aacaggacga ggaaaagtta ttcttcgttc gaaaacaaag atagaggcaa
    46501 tgaagggtaa taaacagcaa atcgtgatca cagaaatccc atatgaagta aataaagcga
    46561 ccttggtgaa aaaaatggat gagatccgtt tgaataagaa aatcgatgga atagctgaag
    46621 tacgagatga aagtgataga acaggtctgc aaattgtcgt cgaattgaaa aaagacgcca
    46681 atgctcaagg aattttgaat tatttgttca aaaacactga gctacagatc aattacaatt
    46741 tcaatatggt cgctatcgac catatgactc cgcatcaagt tggactgaaa gacattttaa
    46801 gaagttatat cgagcatcga aagcaagtca taacaaaacg tagtcaattc gatttagcca
    46861 aagctcaaaa aagacagcat atcgtagaag gattgatgaa agctttatcg attctggatg
    46921 aagtaatcgc aacgattcgt gaaagtaaag acaaaaaaga cgccaaaaag aatttagtcg
    46981 acgtctttca atttacagaa gaacaagcag aagcaattgt cacattgcaa ttataccgtt
    47041 tgacgaacac ggatatcaca gagcttcaaa aggaatccga aagcttgatt gcgcaaatca
    47101 cagagttgaa taaaatcctt tcaaatgaca aagagctttt ctctgttatg aaaaaagaat
    47161 tgcgagaagt gaaaaaaaat tattcttccg ctcgtctaac gacaatcgaa gacgaaatcg
    47221 aagaaatcaa aattgatacg caagtcttag tggcacaaga agacgtaatt gtcagtgtga
    47281 cgcgagaagg ctatgtgaag cgaacaagcc ttcgttctta tagtgcttcc aaaccagaag
    47341 aaatcgggat gcgagaaggc gactacttgc tatactcagg tgaattgagt acattggacc
    47401 atgttcttct gatcacaaat aaagcaaatg tgatctaccg ccctgtccat gaactacccg
    47461 atttgaaatg gaaagatgct ggtgagcaca tttctcaaac aatctccaat ctgtctgtag
    47521 acgaatcgat tttagctgtt ttcccatatc aaaaaattca agcagagaaa acatttgtat
    47581 tcatcagcaa gaatggattg atcaagcaaa cacgaatgac agagttcgaa ccatggcgca
    47641 cctataagag ccgtccgctc agctgtatga agctaaaggc ggaagaagat gaactgattg
    47701 ctgtgtatct gaaagaagat cagccaggct tagatgtttt cttagtaacg catcaaggaa
    47761 tgggtttgcg ctatccttta acagatgttc ccgttgttgg agcaaaggca gctggagtga
    47821 agtctatgaa tttgaaagat ggagatagga ttatcaacgg gctgcttgtg ttagcagaag
    47881 gtgatacgcc gatcgtaatc gttactcagc gaggagctgt taaacgcatg cttgctcaag
    47941 aaatcagcca aacttcacgt gcgaagcggg gagtgacggt catgcgtgag ctgaaaaaaa
    48001 atccgcaccg tattatctat atgtcggaag gaagatcaaa agaaataacc ttgatgaatc
    48061 aaaaagggca gcagttaacg attgacccaa ctgatttccc tataggtgat cgaacatcga
    48121 atggatcatt tgcgatggat gagaaaaaag gtggagaagt catcgaagtt atcgatgctc
    48181 ctgagatttt catcgagtcg taactgttgt agtattttaa tgaagagacg ccatctgtat
    48241 taatacaggt ggcgtctctt tttcattcat ttttaataaa tccttatacg tttgcaaaaa
    48301 aagatgaaag tgttcacaat gcttaaatgt tggtatgtca acgttgattt aaatatatgt
    48361 aaataaaaca taaaaattgt taaataaaaa acaagaaaac ccttgcaaag atagggtgct
    48421 gtgttatact aatcacgtaa aaactgttac ataaaacaga aatttaggag gtccatacaa
    48481 tggagcaatg gaatggattt aaaggtacca aatggaaaga aggcgtagac actagagact
    48541 ttattcaaaa taactacact gaatatcgcg gtgatgacag ctttttggaa ggcccaacag
    48601 aagcaacaga aaaattatgg gatgaattgc aaaagttaaa tgatatccaa ttcgagcgta
    48661 acggtgtcta tgatatggac acagaaatcg tatcaactat tacttcacac gatgcaggct
    48721 atttggataa agatttggaa aaaatcgtcg gtctacagac agacaaacct ttgaaacaag
    48781 cattcatgcc gttcggcgga atcaatatgg ctaacaatgc gctaaaaagc aatggctata
    48841 caccaaacga tgaattgaca catattttca ctgactggcg caaaacacat aatcaaggtg
    48901 tatttgacgc ttatactgct gaaatgcgtg ctgctcgtaa aaacaaaatc attacagggc
    48961 ttccagacgc ttacggtcgc ggacgtatca tcggtgacta tcgtcgtgtg gcattgtatg
    49021 gaatcgacta tttgatggaa caaaagaaaa aagatcatga tatgactggt tatcatacaa
    49081 tgagtgaaga agtgattcgt ttacgtgaag aaatcactga acaatatcgt gcattagcgc
    49141 aaatgaaaga aatggctgcg aaatatggct atgatatttc taaaccagct gcaaatgcaa
    49201 aagaagctgt ccaatggcta tacttcggat atttagcagc aatcaaatca caaaatggtg
    49261 ctgcgatgtc aatcggacgt atctcagcat tccttgatat ctatatccaa cgtgatttgg
    49321 ataatggagt aatcacagaa aaagaagcac aagaattgat cgaccactta gtaatgaaat
    49381 tacgtatggt caaattcgcg cgtacacctg aatataacca attattctca ggattcccta
    49441 tctgggctac tttatccatt gcaggtatgg gattagatgg tcgttcatta gttacaaaga
    49501 atgatttccg tctgcttcat acattaacaa atatgggacc ttcaccagaa ccaaacttga
    49561 ctgttttgta ttctgaacac cttccagaag gattcagaac atatgctagc agaatcgcta
    49621 tcgaaagttc ttcgattcaa ttcgaaaatg ataatcagct tcgtgaacaa tggggttctg
    49681 atgattgtgc gattgcttgc tgtgtatcag gaacagttgt cggaaaagac atgcaattct
    49741 ttggcgctcg tgctaacttg gctaaaacac tattatatgc catcaatggc ggagtcgatg
    49801 aagtgactgg cgcccaagtt ggaccagaat atcgtccaat cacatcagaa atactagact
    49861 ttgaagaagt gaaacataac ttcatgaata tgatggattg gttagcagaa ctatatgtca
    49921 atacgttgaa catcattcat tatatgcatg ataaatattc ttatgaagca gctcagctgg
    49981 ctttgatgga ttctgacttg caacgcacat ttgctacagg tattgctggg atctctcatg
    50041 cggcagacag cttgtcagcg atcaaatatg caaaagtaaa agcaatccgt aatgaaaaag
    50101 gaatagcagt tgattttgaa gtggatggcg acttccctaa atacggaaat gatgatgacc
    50161 gtgcagatga attagtttca tggatcttgg aatacttcat gggacaaatc aaacgtcata
    50221 aaacttaccg caactcaaaa ccaacaactt cattattgac gattacttca aatgttgttt
    50281 acggtaaaaa tacaggaaat actcctgatg gccgtgaagc tggaaaacca cttgcgccag
    50341 gagcaaatcc aagttacaat gcggaacaaa gtgggttatt agctagcctg aactcaacag
    50401 ctaaaatgaa ttacggctgt gcattggatg gaatttcaaa tacccaaaca atcaatcctt
    50461 caggtcttgg aaaagaacaa gatacaagaa tcgaaaactt gcgtaatgtc ttagatggat
    50521 acttctcaaa aggagcttat catttgaatg tcaatgtatt cagtaatgat ttattgcgtg
    50581 atgcaatgga aaatccaatg aaatatccga acttgacgat ccgggtatct ggttatgcag
    50641 ttaaattccg tgacttgaca agagaacaac aattagatgt cttgatgaga acttcacacg
    50701 acagaatgta aaaagttcgt gaaaaaagtt tttcgactta gcgtcgaaag gacgttgaat
    50761 agccatcgca tcaaaatcga ataatcagta tgcttatttg aaagaaacga tttacagggg
    50821 caggacttgt atgttccgtc cctgtaaatt ttacacataa cgaagcggca attgcgaaaa
    50881 attttgttca gaaagcttcg cttcgacagc taacttgtac ttaaaatgaa atcaaagaaa
    50941 agatgtgaac ttaaatggaa gaaaaaacaa tcggctatgt tcattctatc gaaactttcg
    51001 gttcagtaga cggacctgga ctgcgctttg tcgtgtttat gcaaggatgc cgcatgcgtt
    51061 gccaattttg tcacaatcca gatacttgga atattggcgg agggaaagaa tacacagcag
    51121 atgaactttt agataaagca gaacgcttca gaccttactg gggagataaa ggtggtatta
    51181 ctgttagtgg tggagaacct ctattgcaga tcgatttttt gatcgaacta tttaaaaaag
    51241 caaaagagcg gaagatgcat actacattgg atacatgcgg aaaacctttt acgtatgaag
    51301 aacctttctt ttcacgtttt caagaattga tgaagtatac agatttactg ttatttgaca
    51361 tcaaacacat cgataatgaa gagcataaaa aactaacgca ttgggacaat gaaaatattt
    51421 tagaaatggc tcaatatttg tcaaaaatca acaaaccggt ttggattcgc catgtactag
    51481 ttcctgagcg aagtgattat gatgaatatt tgatccgact agacaacttt attcaaacct
    51541 tgtcaaatgt agacagagta gagattcttc cttatcatac gatgggaaaa tacaagtggg
    51601 aaacacttgg tttgaaatat ccattagaag ggatcgaacc acctacaaaa gaacgtgtcg
    51661 aaaatgctaa acgattgctt catacagaag agtatacaaa atatttaacg cgataggtcg
    51721 catcggaaag aagataagta ttgcttcttt ccgttttttt gctattatta aataggagaa
    51781 atcacaaagg agcgaatgac atgtcaaaaa ttttagtttt tggtcaccaa aatccagata
    51841 cagatgcaat cggggcagcc attgcgtttt cacacttgca aaatgaatta ggtaaagaaa
    51901 cagaagcagt tgctctaggt acacctaatg aagaaacaca atacgcatta gaccactttc
    51961 agctatctgc accacgagta gtagagtcag ctaaagcaga aaatgcagat gtcatgcttg
    52021 tggatcataa tgaattccaa caaagtattt cagatatcag cgaagtaaat attttagctg
    52081 tagtagacca ccatcgtatt gcaaactttg aaacagctaa tccattgtac tatcgtgcag
    52141 aaccagttgg atgtacaagt acgattattt taaaactata taaagaaaac caagtaacaa
    52201 ttcctaaaga aatcgcagga atcatgctct cagctatcat ttcagataca ttgttgttca
    52261 aatcaccaac atgtacgcct gaagatgaag cagctgcaaa agaacttgca gcaatcgctg
    52321 aagtcgatct aaacacttat ggacttgaaa tgttgaaagc aggaacaaat ctaagtgata
    52381 aatcagctgc ggttcttctt gatttggatg caaaaagttt cccaatgggc agttccaacg
    52441 ttcgtatcgc tcaagtcaat acagtggacc ttaatgaagt gatggatcga caagcagagc
    52501 tagaagcagc aatggaagat gaaatgaaag caaatggcta cgatctgttc gtattagttg
    52561 tcacaaacat cttagacagt gattcagaat tacttgttgc tggtaaagga aaagaaaaag
    52621 tagaagaagc tttccaaaca actttgacca acaaccgtgc tttgcttaaa ggtgtcgttt
    52681 cacgtaaaaa acaagtcgta cctcaattga cggctgtatt tagctaatca aatggtagta
    52741 tgaagcattc aaaatgaatg gataagcgat tgatataaaa agaaacatag aacatactaa
    52801 aggccgaaga aatggaggag aataacagtc aattatgact cttgttcttc ctccttttta
    52861 cgtaaaagcc ttttaagtaa aataaaacgg atagaaaaga gaacatatat gaactattat
    52921 tcaaaaaata aggaaaactt ttatcaaaaa ctgaccgatg atccgctttt ttcttcattg
    52981 accgattatc tatatgaaca tcgagaacaa gaaacgattc taagagaatt gaaaaaagaa
    53041 ttttcgcaaa ataaattttc ccattttctt gatttattga tcgatgcagg attaatcaag
    53101 agggaagaac gaagatatca tttgaacttt ccaatatttg atcctaaaga ttacttgcaa
    53161 caagccactt cggctgctga aaccatagct gaacaattga agagactctc tgttgatgaa
    53221 caaaaattag cgatgggaga agttatttgg gcgtattgtt tcgaagatga acggaaagaa
    53281 gcgtattttt atggagtaag aaacagccga gagacagaat tgctgaggac aactgcagga
    53341 aatgagaagt accgttttat cacactttcc tctatagaac acttcccgct tactttggct
    53401 aattactttt ttgtacagaa gaatcagctt cctgtaacga aggcattcaa agaattagcc
    53461 gaacttattg gagacgtaaa tgaatcgtac ttttttgatc aaatagaagt tatcgttgac
    53521 aggatacgta agaataaata taaaaatcgc cggcctagta ttttccatca gtctctactt
    53581 gtgacgaata ctataaagga agaagaatca ttcacattag aattacctat tgtcgaaaaa
    53641 aacaattttg agattgaatt accaacattg gatccttcat tgacaatgga ggaaatggcc
    53701 tttcttaaac gacaaatctt ttctgagctg agcaagaaat ttataccgca tgcattttct
    53761 tatatcaaag aatatggaac agttttagtg agtaaaacct aagagagcag taagcaaaaa
    53821 ttatactgac tcttgcagga ggagcctttc tcttccttta tggttaaaag aattatttga
    53881 gtattttgcc tatctaccac ctattagatt cactacatgg tctggaaaca aaggaatgca
    53941 aaaaaatggt gagaaatgtg gaatgacaaa ggaagtagtg ataagaaaag ttagattttt
    54001 aaataatcaa tattatgatt cagtcaagta tggcatttta tgggaagaac tagctgatta
    54061 gattaggaag tccatttgat aatccagaat cactaagaaa cgaatggcga gtcattaata
    54121 atttatccaa tcttgggtta ttcttttaga ttgaagtgtt taaaactaaa aaagcattta
    54181 tgaagtcgta atagtcaggc ttcaaaatgc tttttatttt gtttgttttt tctttgccac
    54241 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
    54301 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
    54361 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
    54421 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
    54481 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
    54541 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
    54601 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
    54661 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
    54721 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
    54781 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
    54841 taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg atgtttttta
    54901 atcaaaa
//
LOCUS       DACEOM010000226        16870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.070, whole genome shotgun sequence.
ACCESSION   DACEOM010000226 DACEOM010000000
VERSION     DACEOM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.070"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            462..1325
                     /locus_tag="IW781_003135"
     CDS             462..1325
                     /locus_tag="IW781_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1184072.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1425..1907
                     /locus_tag="IW781_003136"
     CDS             1425..1907
                     /locus_tag="IW781_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1184073.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2170..2637)
                     /locus_tag="IW781_003137"
     CDS             complement(2170..2637)
                     /locus_tag="IW781_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1184074.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            2760..3611
                     /locus_tag="IW781_003138"
     CDS             2760..3611
                     /locus_tag="IW781_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1184075.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(3862..5820)
                     /locus_tag="IW781_003139"
     CDS             complement(3862..5820)
                     /locus_tag="IW781_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1184076.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5817..6689)
                     /locus_tag="IW781_003140"
     CDS             complement(5817..6689)
                     /locus_tag="IW781_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1184077.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(7031..7903)
                     /locus_tag="IW781_003141"
     CDS             complement(7031..7903)
                     /locus_tag="IW781_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1184078.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            8031..8600
                     /locus_tag="IW781_003142"
     CDS             8031..8600
                     /locus_tag="IW781_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1184079.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8667..9404
                     /locus_tag="IW781_003143"
     CDS             8667..9404
                     /locus_tag="IW781_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1184080.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            9635..10903
                     /locus_tag="IW781_003144"
     CDS             9635..10903
                     /locus_tag="IW781_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1184081.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            11390..11854
                     /locus_tag="IW781_003145"
     CDS             11390..11854
                     /locus_tag="IW781_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1184082.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            11838..12467
                     /locus_tag="IW781_003146"
     CDS             11838..12467
                     /locus_tag="IW781_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1184083.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            12821..14299
                     /gene="msr"
                     /locus_tag="IW781_003147"
     CDS             12821..14299
                     /gene="msr"
                     /locus_tag="IW781_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1184084.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            14346..14792
                     /locus_tag="IW781_003148"
     CDS             14346..14792
                     /locus_tag="IW781_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1184085.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(14991..15506)
                     /locus_tag="IW781_003149"
     CDS             complement(14991..15506)
                     /locus_tag="IW781_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1184086.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            15623..16824
                     /locus_tag="IW781_003150"
                     /pseudo
     CDS             15623..16824
                     /locus_tag="IW781_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         5340 a         2990 c         3498 g         5042 t
ORIGIN      
        1 gaatgtgaga aaaagtaaaa gaatagcgtg ttttaaagat ggaacttttt ttgtttttta
       61 tttatctgac aaaaatgaag tgattttgtt agaaataaat aattgttcca attgataaaa
      121 gaggtatgat gtatcgcaaa gaaaaatcca agcagcgttc tgatcagcag tttgaacaac
      181 aatttgcttt gtacagtagg tagtaagcgg attaactaag ggaataaaag atatttttac
      241 cgctttgttt acttagaatc tgtcttcccc aaaatttatt tttctaaagg taaaacatag
      301 ataggcagat aataccaatt atccgttacg aaacgctgct tgagaatttt ctttagatcc
      361 atacaacatt ttctacatct gactaaattg gttcctgatt catcaagtaa ttgactttct
      421 ttgataatat gataaaaagg caatcaagga ggaagataaa tatgcttaaa gatctgaacc
      481 aattgatcga ttacatcgaa gaacatttga cagaaggact ttcctataaa ataatagccg
      541 aaaatacagg aatctctgaa tatcaattaa aacgcatctt tacgtttatt gcagggatgt
      601 cgctaatgga ctatatcaaa aaccgtagat tggctttggc taatcgcgat cttctagatg
      661 gatcaagagt gacagaaacg gcgtttagat atggatatga aacagtcgaa ggtttttcaa
      721 gagcttttcg tgattggtca ggatatctgc cttcagaaat cagtagatac ggcatacaaa
      781 aatcttttcc acgcttgaca ttttttattg atgtcagagg aggaaaatcg atggaatttc
      841 gtatcgaaaa gaaagaacag ttttatgtgg caggtgtggc gaaaaaagtt cctgtcaaat
      901 ttgaaggagt caatcaagaa atacaaaagt tagcgcagac gatcacggag actcaaaggg
      961 aagagatgca taagttagga gatttatatc caaatcaagt aatcaacgtt tcttatggat
     1021 ttgatggaga ccgcttggaa gaaaagggca gtttgatgca catgattggc tttgctacga
     1081 caaaagaaaa tccgtacgct gatttagaaa tgcttgctat agaagaacac acttgggcca
     1141 tttttcctaa taaagggcca tttccccaaa ctttacaaga tacttggggg aaaatctatg
     1201 cagaatggct tccttcttca gattatgggt tagtcgaagc tcccgagatc tcttttacaa
     1261 aatggggaga ggatttcagt aatgtttaca gtgagatttg gattgcagtg aaagcaaaaa
     1321 aatagttaaa aaataaaaat atccctagaa aaaagagcgt aatcgtgtca tttcccttta
     1381 attttatgta agatgaactt aatataatag aaagggtggg agaaatggca aagtatagga
     1441 tcgaagaaaa accagatttt acggtgacgg gtattggtgt ggaattgaag agtagcaata
     1501 cggattttgt cggtttggct gcagagaaac aagcatttga gcaaaaagtt atcggtaatg
     1561 gaacaataga tgcattgaaa caagctgctg aaaatcatta tttctttaca gtcaacgaac
     1621 cctataacgg gaaaatgatg tactatgtcg gtgttcaaac gagtcaggat gttccaaaca
     1681 ctacgcgaat catcccattc ccaaaaggaa cgtatcttgt tgtaagcgct ttagcagaat
     1741 cgcctgaaga attaagaaat gatttgacga atacggcctt tgggcatgtt ttaagagaag
     1801 tagaagatta cgcctatgtt gggggtccaa atgctgtagt cgaaatggga gttaccaacg
     1861 ggaaatatat gggagaaatc tggataccgg tagtcaataa accatgataa tatagaggtc
     1921 gtgaaagtag cgctttgacc tgagcattag agaggaaaaa tgggaaatag ctttccatat
     1981 tttgcatttt tcagaactaa tgccgagggt cagctacttg aacaccgttt atcaagacgt
     2041 catgacttca gcgctttgac ctgagcatta gagaggaaaa aggggcatga caaggctttt
     2101 gtcccccgga gaataaaaga atttatgtag acgattccga tcagaaaaat gcaggcacag
     2161 tagagagaat tataaaaatc tctctacgag gcctgtgttt tctggtcttt tttctttttc
     2221 caaaacggct attttatgta tcagactatc aagtgtgatc ttagccattt cttcttctgc
     2281 ctttttctgc agccgagcgt agctttcttc taaagcttct tggatattcg caccaacgac
     2341 gcaatttgga tttgtttttg gatcgacttg gataagctgt gtattacctt cgatgctgcg
     2401 atagacatct agtaaagaaa tctgatctgg acttttggca agggaaggct gcggttttcc
     2461 ggtttttgta aagatcaaac cggatttttt gagctgactc atgattttac gtacatttgc
     2521 tgcatttgtt tcgatactac tggcaatcat ctcactagaa agtgatcggg tatcttgaaa
     2581 catttcaata taggcaagta catggattgc atcactaaat tgcagagaat acttcatcag
     2641 ttgtcatcct ttcatttttt ttgcttgact tatcttacca cactctttat gataaaggtg
     2701 taatttataa atgtacagct gtaaatctta acaaataaaa agaataggga gtggcaaata
     2761 tgcattattt agtaacagga gcaactggag gttttggtgg ttatgcgctg gaattcttaa
     2821 aaaagatggt tccacaaaat gaaatctatg tactagtacg tagtgaagaa aaaggagaag
     2881 cactgaaaga agctggattt aatatacgta ttggtgatta tgatgattta gactcaatga
     2941 aagaagcgtt gaaaggaata gatcgattgc tttttgtctc tggcgttcca ggaaatcgtc
     3001 aagccgaaca taagaatgta gtgaaggcag cacaagcagc tggggtatct tatatcgctt
     3061 atacaagttt tgcagatgcc gatcactcga tcagtgtatt agcgccagat caccaattca
     3121 ctgaaaaaat gattaaggat tcaggtattt cccatacatt tttacggaat aattggtatg
     3181 tagagaatga attacctatc attgggcagg ctttaaaaac tggtcaattc gtttatgctg
     3241 caggaaatgg aaaaacaggt tgggcattga aacgtgaata tgcagaagct gcagcaaaag
     3301 tactagtaga aagtgactct ccagaaatct tagaattatc cggcaatcta actgactatg
     3361 aagaattagc caaggcatta gagcgtgcga caggtaaaga gttagaaatt attgaagcaa
     3421 gtgatgcagc gtttgtcgaa aatctaaaag aagccggatt ccctcaagaa gcagctgaca
     3481 tgtttttagc gatccaacat gatattaaaa aggatcagtt agctatttcc tctgatgatt
     3541 tagaaaaagt attgggaaaa ccattaactt ctcttgaagc agcattaaaa gaaatattgg
     3601 atcaggaata atgtataaag aggttgtgaa aaagccgatt agttttgagt atgaagaaaa
     3661 attttgtgaa aaagctgttc tgcatcaagt aataagaaga gtcaaataaa aaatagctct
     3721 tcatatgttt cgtttagctg gacttattac cgcggagttg gcaggagaac gccgttttta
     3781 ttcggaaaaa ggagctgaga caagattttt gtctcagctc ctgtttgttt atcgataaaa
     3841 aagtctgggg aagacgatgg ctcaggcttt gttggaaaca gagccgattt tatcatttgc
     3901 ttttacatca cgtgcgggtt cgatttctaa gctttggact ttgtcgccat ttgtgaatac
     3961 aacgaccata tccgtgtttt tgcctgcaga ttgaatagcg gcaagatcaa ctaaagcaat
     4021 caattgacct cttgcgactt tttgaccttc ttctacatat aaagtgaatg gttcgccttt
     4081 taagtcaaca gtgttgatgc ccatatgaag caagacttcg ataccagcat ctgtttggat
     4141 tccgacagca tgtttcgttg ggaaaatgtt tgtgatcgta ccagcgattg gagagaagat
     4201 ttctccattt tctggtaata cagcatagcc gtcacccatc attttttccg cgaatacatc
     4261 atctgaaact tcagtgattg gaatcagttt cccatttgcg atagcgtaaa tatcttgttc
     4321 gatcgtttca ccggcaaatg tttctgtttt aaccacggtg tttgtttgtg caggtcgtgc
     4381 tgcagcacca ccatgtagtt ttgccatctc atctgcaacg aattgtactt ctgtacctac
     4441 gatgacttgc gcattttttc cgtcgatgat cttcatacca ggtacgccag tggctttgat
     4501 tttattttga tcgatcaggc ctgtatcttt gacttgcaaa cgaaggcgtg tagtacagtt
     4561 atcgatcgaa gttacattgt cggctccgcc taatgcagcg tagattttgc ttgcttgtgc
     4621 cgcatattta ttgtctgaat tagtcgttgc agtatcgtca tctgaaagat ctgcttcctc
     4681 gcggccagga gtcatcagat ggaatttttt gatagcaaag ttaaagccaa agtagtaaat
     4741 cactgctgtt actaatcctt ggacgatcaa catcaatggc tgattcgcaa tcggattttt
     4801 caaagatagg aagtaatcga caaatccggc actaaaggca aatcctgctg tccaatggaa
     4861 gaatgcactc actgctaaag aaatcccagt gaaaattgca tgcaagacat ataatggcca
     4921 agcaacgaac ataaatgaaa attcaagtgg ttctgtcaca ccagtaaaga atgaagcaaa
     4981 agctgctgct agcatcaagg aagctgtttt tgctttgttt tctggacgtg catttctgta
     5041 aatagcatat gctcctgctg gaagcccgaa catcatgatt gggaaaaatc cagcttgata
     5101 cattcctgtt acgccttttg ttccttcaga agccaaaaat ttaccaatat cattgattcc
     5161 tgctacgtca aaccagaaca cggagttcat tgcttgatgc aatcctgtag ggatcagtaa
     5221 acggttgaag aagccataaa gacctgcacc accccagcct aaactcaaaa tgaatttagc
     5281 aaaagaaact aaagcagagt aaaccggagg ccatacgagc agtagcactg cactgatcag
     5341 caacatgaca aaggcagcca agattggcac tagacgcttg ccgctaaaga aagataaagc
     5401 catcggtaat tttgcttcgt ggaaacgatt ataaagagct gccgcaatca atccggcaat
     5461 aataccaata aatacgttac tgatctgagc aaaagatgga tcaactgatt ctggtttgat
     5521 attcaataaa gtagctactg aatcagtttt taaaacattt actggaactt caaatgcgac
     5581 caaacctgcc aaggcagcag cacctgattt gtccactgac atccctagtg ctaacccaac
     5641 ggcaaacagc aaaggtaatt gacctaaaac tgcgttaccg ccagcttgta aaaaatgagc
     5701 gacaatgtcg ttactataag atgcccacca gtttccgatc cctactagta aagcagcagc
     5761 aggtaagacc gcaactggaa gttgaaggga acgtcccatt cgttgtagat atgctttcaa
     5821 tttttttcct cctcatgatt gttaacattt gtttgtacga ttgcgcttct taaacgctcg
     5881 atatggatgg ccaagtaacc cagttcttct ttcgtgatcg cgagctggta ttctttggtc
     5941 agcaacacac ggattttttt agaaatctta tagctttctg ggtattttcg tttgaccatt
     6001 tctacgattt ccgtgtctaa aacagcatat tgttgcgcat gggttcgttg tagaagcagt
     6061 cgtaaatgat tcaccagtct tgaataattc agtgaaaatg gttctgaatg gatatctgtt
     6121 tccaattctg attccactaa atggatgatg tcagatacaa tcgtcacttc acggatactg
     6181 cgatggttat tgttccgacc gcttcgtccg ctgtgaatat ggattgcgat atatcctgct
     6241 tcgtcataag tatatggaat cccaagctct tttgtcagat actctactgc ccattgagca
     6301 atactgaatt catcactgta aagcacttcg atttctctta aaagtttgtt gcggatgacg
     6361 ataccgttat taatgttctc cgcagaaaaa gcaatgtggt cggtcaaagc gatcaataca
     6421 tgttcattca acttttccat caaaacagtt tccgcatgat caatgatcgt ttctgctgca
     6481 aataaaaagc gttcatcaat ctgattaagt aaattttgca acttgatttg accttctggt
     6541 tccatgacaa atatccgttc gacatcacga tcaaaaatca aatcatttct ttttctgtca
     6601 aatccaatcc cttttccaat agctactttt tcctgtccac cgtcatctac caatactgca
     6661 ttttgattaa agatcttttt gattttcatg cgcgcaccct ttttgaagat ttggttttca
     6721 cgtttgctag accagttgat tgcggccaaa caaaaataga cacgaaaaac ctcctaggga
     6781 aaccatgttc ctcagagatt tttcatgcct agtatgatct agtcacatga aacgaattat
     6841 tgttaacgta atcaatttta tatagacagt tatgttttgt caattctttt tttataatta
     6901 tagtaattta ctggaattat cagaaataaa aggaagaaat aaattaagaa acttatgtta
     6961 tttaatcgtt ggatcttttg cttatcttat tcgttgttaa aaagctgaaa gcgaaggaaa
     7021 aaaactctac ttaactgtat ttctccgtta ggacatctaa aaatttacga gcagcaggag
     7081 aaagaggctg gtcttttttc caaatgagat tcagtgaagc ttgtaatggc ggatcgagtg
     7141 ggatgaatat tttttccgtc ccataagtat tgatgatccc atctagagct aatgcaccaa
     7201 aagtcccgtt atcgaccatc aaagaagcat tgtacaataa gttatatgtt ccgacgatcg
     7261 ataactgatc tatgctgaaa cctagccatt cagctagttg actgtccact gacgtttgcc
     7321 gggaaatgat caaaggaaca gttttcagtt cctgcggatg gatgacggtt tgctttgcta
     7381 aaggatgatt tcgatgtatc aaaagtcccc aacgatcttg ggctggcagt cgaaatgact
     7441 ggtatttttg tttgtttgtc ggttcgatta cgagtccaaa atcaagtaat ccgctatcca
     7501 gtttgtctat gatatcatct gcattcccgc tatataaatg aaactggata tcaggatgcg
     7561 ccttgatcaa cgaatgaagg ctatcagaaa tgaaagagat tgctttagtt tcgcctccgc
     7621 caatgtagat ttctccagag atgattgcgt catttgttaa attgttcgcc gttttttcta
     7681 ccaacgacaa gatttctttt ccttttgaat aaagatatcg accagaatcc gtcaactgta
     7741 tctctcggga gccccgagtg aaaagtgtca caccaagttc ttcttctaaa tctttcagtt
     7801 gttttgagag agtaggttgc gtgatatgca gagattttgc tgctcggctg atattttttt
     7861 ctctagctac tgctaaaaaa tacgtcagta tacgaatatc cattttttcc ctcctgtttt
     7921 taaaaaagca taccactaag tatagttaaa aggaatagtt aagtatgttt tataagtatt
     7981 agacatgata agccgtccgt tataaagtaa ggataaaagg agggagcaaa atgacaagcc
     8041 tcatcgaaaa acaaatcgtc aataaagaga ttcgagtaac ggatccgctc tttgaagtga
     8101 tccatcagat ccaagctgaa aacgaagagc ctttagcagc gctgaatact gggtaccatg
     8161 aaccagcaga tatccgcaaa cggctagaaa atatcattag tgacaaagta gatgatacgg
     8221 tgactgttct gctgcctttt tatacagatt tcggcaaaca tattagtata gggaaaaatg
     8281 tctttatcaa tcgtcaagtg atgttcgttg acttaggtgg catctgttta gaagattcag
     8341 tattgattgg tcctcgtgtc aacctcatca cggtgaacca tctaaccgat ccaaaagaac
     8401 gaagaggctt gtcagtaaag ccaatccaca tcaagaaaaa tgcttggatc ggagcaggag
     8461 caactattct gccaggcgta acgattggcg agaatgccat tgttgcagcg aatgcaactg
     8521 ttacgaaaga tgtcccagac aataccatcg ttgcaggaat ccctgccaaa atcgtcaaac
     8581 ctgtcgaacg gattatttga acagtaagat gaaataagag atagtgagta taagcaagag
     8641 atttttcaaa acaggaggag aatagaatga atgatttatc agaaaaagtg attgtcatca
     8701 tgggcgcttc aagcggaatc ggtgaagcaa caacgaagct attagcagaa aaaggggcta
     8761 aactagttat agctgcacgc cgagaagatc gcctaaaagc aataaaagaa tctttaccag
     8821 aagctgagct atatattcaa acagcagatg taagagattt tgcacaagtc caagcagtta
     8881 tcgatttagc gatggaaaag ttcggtcgaa tcgatgtatt gtacaataat gcaggaatca
     8941 tgccaaccgc tccattagtt gaaggtcacc gtgatgaatg gcaaaatatg cttgatatca
     9001 acatcatggg tgtgttaaat ggaatttccg ccgtacttcc aatcatggaa aaacaaaaat
     9061 ctgggcatat catttcaact gattctgtgg cgggacatgt tgtttatcct gattccgccg
     9121 tttattgcgg aacaaaattt gctgtccgtg caatcatgga aggattacgt caagagcaac
     9181 ggcaaaataa catcaaatct acgatcatct ctccaggagc tgttcaaact gaattatatc
     9241 aaaccatcag cgacaaaaag gctgctcttg aacttcatga agcgcaaaaa gaatggggat
     9301 tgacctccga agatatagct tctgctgtag catttgcgat agaaacgcca gaccggatgt
     9361 ctgtcagtaa catgattatc cgtccgacga cacaggaagt ataatccata acggataata
     9421 tccaaagaga attacttgtt aaatagttat tcaaacgacc atcaatttaa ggcgtgaatg
     9481 gagaagaata ttcttcattt acgtcttttt atgtagaaaa agcaaattta tcaaaaagaa
     9541 acaaatgtta tttaaatatg gacattgact tgcttttact tttttagtta cactgttatt
     9601 taggaaaagt ttcataattc aaaggagaaa aaagatgaaa gcaaagaatt tattatttag
     9661 cgttattctt ttaggaaatc tagcagttgc caacacaaca gtgctagctg aagaatcgac
     9721 agaaactgtc gcaactcaga catcgagttc ttcagaaaca ggaattgaaa acgatacaga
     9781 gagtactttg cagtcagcta ctgtctcaga aaatacaatt tcaaccgaag aaccaacaac
     9841 tgaaacaaca gaacaaacac aaaccgaaga cagtacatca gcagaagctc agaacaatcc
     9901 agagaatgca gaagaaatca cgaaccaatt gacagatggg caaacattga cacataccca
     9961 tcaagcagat ttgtctgaat tcgaaaacta tactggctat gctttttatg ctcgctcatc
    10021 tgctaattct caacaagcgt tcattgatag tattgcctca actgcacaaa gtttggcaag
    10081 tgccaatgac ttatatgcat cagtcatgat tgctcaagcc atcgttgaaa gtggctgggg
    10141 gaacagtact ttggcttctg cccctaatta caatctattt gggattaaag gaagctataa
    10201 tggccaatct gttacgatgc ctacttcgga atatgtgaat gggcaatgga ttacggtgaa
    10261 tgccgctttt agaaaatatc cttcgtacaa agaatcattg caagataacg taacggtatt
    10321 gaagacgact tcttttcagc caggcgttta ctactattcc ggtgcatgga aaagcaatac
    10381 gaactcttac aaagatgcta cggcttggct aacaggtcgt tatgccactg cgcctaatta
    10441 tggttctaca ttgaacaatg taatcgaaac ctataatctg acacagtatg atacagcacc
    10501 aacttcaact tctccgatgt atcgtctgta taaccgtcat acaggagaac atttatatac
    10561 attgaatgcc ggagaaaaaa attatctgcc aactgtaggc tgggaatatg aagggattgc
    10621 ttggcaagca ccaaacagcg gacaacctgt ctaccgttta tacaatcctt acagtggaga
    10681 ccatcattac acaatggcac aatctgagat caactcttta acaaaaatcg gttggcgtta
    10741 tgaaggtcta agtttttatt ctggtggttc caagccaatt tatcgactat ttaatccaaa
    10801 tgaaaaaact ggtacccatc actatacctt gagtgctaag gaaagagatt ggctaacacc
    10861 gatgggctgg agatatgaag gaataggatt ttatggttat taaaaagagg tagtgaaaaa
    10921 gctgccctgc atcaagtagt aagaacagcc aaacaaaaac agattttcat gttttttgtt
    10981 tggttgaact tattaccgca gatgcaagtt tttgaacacc gtttactcaa aaaaggttgt
    11041 gacaacatct tgtcataact tttttattac tgtttaagca tactgatcca atttctaata
    11101 ttcgacggga ttcttgcaaa aggcgatttc ggaaattttt ggcggtgtaa aattttctac
    11161 tgcagaagag ctatcttcta tagtttgata agaatcaata atcttggttt tagcgttgtg
    11221 ctttctttat cattttttcc gttcattcta caaaaaattt cttttctcta tacttttcta
    11281 ttgagagcgt tttttttatt acacagctga ttctttgact tttttctttt tttattctat
    11341 tattgacaag tcaatcgttg acgagtcaat tattagaaag gaggaaatta tgtctttaga
    11401 agagattagt caccttttat atcaaatcaa attagccgat caaaaaatcg tccagatctt
    11461 tgaagaaaat atcggtataa gtcttactag atacgagatc atgatgagac tgaaagaaga
    11521 aaatccattg ctacaaagca gtttgcaaga aaagttgcag attgatcaag gagcagttac
    11581 tcgtcatctg aaggtattgg aagcaaatgg ctatgtttcg cgtcagagaa atcctcagaa
    11641 caaccgggaa gtgtatgtag cgctaacaga aaaagcccag aagaaaatcg agcattgtga
    11701 aatggaccat aaagatacag gttccgtgct atccccggct ttcagcgatg tagaaatgaa
    11761 acaatttcta acacttttag ataaattgaa tacccaactt tgcaatataa caacagaaac
    11821 gaaagaggtt aataataatg gaacaaaaaa atgatttttc aacaattatg actggtagaa
    11881 gatccgttcg cgtatatgat gaaacataca aaatcccaca cgaagaaatg ctggaaatga
    11941 tcaaagaagc gacgactgca ccttcttctg tcaatatgca accatggcgc tttgtggtcg
    12001 ctgaaagcga ggaagcaaaa gaaatcttgc gtcctttgat ccgtttcaat acgagacaaa
    12061 atgatacttc ttctgcaatg gtcatgattt ttggcgatat gcaatgttac gaatatggcg
    12121 aagaaatcta taaccaagct tacgaatcag ggaagatgcc aaaagaagtc agagatcaac
    12181 aattagctgc gatcattcct tattacaaga gtttttcaag agaagaaatg aacgatgtag
    12241 tcaaagtcga ttctagctta gcggctatgc aattcatgtt agttgcgcgt gatcatggct
    12301 atgaaacaaa tccaattggt ggattcgaag cagatcagtt ggcagaggca tttggtttgg
    12361 ataaagaccg ttatgttcca gttattattt tatcaatagg aaaagcggta gaagaaggtt
    12421 acgagtctgt tcgtttagca ccagaaaaga tcactacgtt cgaataaaag gttttcagtt
    12481 actattcagt agctattgct ttttttgaat cgtgtgtggt aacctaataa aaaaggaggt
    12541 cggaaaaatg actgcatcga tgaaattacg tttcgaactt ttgaataaca actaaataaa
    12601 ttcaaaagac atgtataaac aacaatttca tggatggagt atgtccattt tttcgaacga
    12661 cagtagaata ggtgaaagct atgacagatt tggtgattta gtcatagtta tttcgttcag
    12721 agtaggaaaa gatgtgatgc agtttttttg caaacagatt tttctgctct tttttagtat
    12781 cgataattgt tgttttacat gaaataggag tgtaaaacat atggaaaatt tagcagtaaa
    12841 tataacaaat ctacaagtca gctttggcaa ccagctagaa ctatctattg attctcttcg
    12901 tgtctatcag caagatcgta tagggatcat aggagaaaat ggtgtcggta aatcgacttt
    12961 gctcaaacta atagccggtg aactttttcc cgatcatggg aaaatccaaa cagagatcac
    13021 cttcaactac ctgcctcaat taacctatct cgctgaagca aaggacctaa atttggaatt
    13081 agccagtcat ttccagttaa gactggaaga aacttcagag cggaaatgga gcggagggga
    13141 agaacgaaag atcgagttga tacgtcttct ttcttcttat gaacaaggga tgcttctaga
    13201 cgagccaaca acccatctag atagaaaaag tattgatcga ctgattgaag agcttcgtta
    13261 ttattatggt acgctggttt ttgttagtca tgatcgctat tttctagatg agctagcatc
    13321 gaaaatctgg gaagtaaaag acggagaaat ccgagagttt tcggggaatt atagtgccta
    13381 tctcactcaa aaggaattgg agaaaaagac tcagctacga gaagcagagt cgatcatgaa
    13441 agagaaaaaa cgattggaaa aatcgatcca agaaaagaaa aaacaagcgg aaaagttgga
    13501 aaaagtgtcc agtaaaaaga aaaagcaaca aatcagaccg gatcggttgt cttcctctaa
    13561 acaaaaagac agtgtacaaa aagccatcca aaagaatgcg aaaacattag agagaagact
    13621 ccaaaaaata ggagaaacaa ccaagccgca gcagatgaaa caaatccgtt ttccagtacc
    13681 aaaatctctt gaactccaca gccgctatcc catcatggga caaaatgtcc aattggaaag
    13741 aagtgggaga acattactgg taaatggtga ttttcagttt tctttaggta aaaaaatcgc
    13801 gattgtcggt gaaaatggtt caggtaagac aaccttatta gaacatatcc gcaaacaagg
    13861 agaaggaatc cttctctctc cgaaagtaag ctttcaagta tatcagcaaa agggttatca
    13921 aatgacatct gaagaatcca tcattcgttt tgtcatgaga caaacagagt tttcagaatc
    13981 gcttgtccgt agtttgctga atcacttagg gtttgctcag gaaactttga cgaaaccgtt
    14041 atgtacatta agtgggggag aagcgacccg tctgacgatt gctttgcttt ttactaagcc
    14101 aagtaatgtg ttgctgttag atgaaccgac taattttatt gatatggcaa cgatcgaagc
    14161 tttagagaag ctaatgcaaa tatatccggg aacgattttg tttacttctc atgattctta
    14221 ctttgtcgag cgtacggctg atgaagttta tgaaataaaa gggcagaaaa taaaaaaagt
    14281 acttacgaga aatttttaaa tgtgctacga tgattttcgt gacaaattta aaggagtggt
    14341 acagattgcc tacaaataaa aaagaaggaa taatttttac aacaatgatg tgttttttga
    14401 tggtcttggg gatgagcgcg tataatttat ggctgcatca agatcttcat ttatcaagtt
    14461 tgttgattgg tctagtgcct ggattcgtgg tggctttcat tttggatgtc ttcatcatcg
    14521 gtgtgctggc gaaaaaaatt gcctttaaac taccagtaaa tccagaaagc aaggtccaaa
    14581 tgatcttaac tatttcatgc ttgatggttt taggaatggt gacatgtatg tctttattcg
    14641 gtgtgatcat ggaacaagga atacctgatc aattattctc tgcttatatg aatgcttgga
    14701 aaatgaatgt gattgccgct ttaccgttgc agttgatcat cgttggcccg ctttcaagaa
    14761 gtgtgctaaa agcaattcaa agtaatgaat gatggaacac tgttatttga aaaaaggcct
    14821 tggcatgctt tttgtcatag tctcttgaat gcagttctag aaaaatagtg atccgaacga
    14881 taggttttgg cctgtcgttc ggatcgcttt ttcttttcca actgtattgc tcttttattg
    14941 aagataattt gctcttcaat agatatttct agaaaggttc tgtttatccc ttactcaaaa
    15001 aactgctgca tttgttctct tacttgctga caccattgga gttcctgctg acaaagcata
    15061 attctgcgct ggatcacaac ataacgtcca aattcttgtt tcttgtctag cgagaggtca
    15121 ttcaattttt tttgattttt ttgcatgatt ttgttcagct gttgttctct agtgaatagc
    15181 agtccagtag ctgttgtttt atcgagcgtc gaaaaagcat agatttttgc tagaaattca
    15241 tctttttgcg taggagcggg agtttcctct ttgatccatt cctctacaag agcacgtcct
    15301 tcatcagtca agtggtagat ctttttttga gagtgctcgt ctttgtccaa aacggtcaca
    15361 taaccctgct ggtctaactt ggcaagcgtt gtatagatct gactgtgatg ggcttcccaa
    15421 aaaattgtga tcagctgctt caattcatac ccagaacgtg gtttggataa aagcacgcat
    15481 aataatatat aggctaaagt attcataaaa atcctacctc tcgtcttatt ttctttatta
    15541 tatcacagta aagtttccac gttttacatg tgcatattta catgtaaaat aaatcatgta
    15601 tgatgctaat agagagaaag ggatggaaat attggagaat caaatagata tacaagaaca
    15661 aacaaatgaa gaaacaaagg ggaaaagttc agcgctagtt gtagcagcta cctgtatgat
    15721 cgcttttatg ggagtgggtc tagtagaccc aatcttaaaa acgattgctg cacagttaaa
    15781 cgctacgcca gctgaaacga ccctgttatt tactagttac atgttagtca ctggtgtggt
    15841 gatgctattt acaggtttct tatctagtcg gatcggtttg aaaaaacatt gatgacagga
    15901 ttgtttatca tcgttgtatt cgctggtctt ggcggattat ctgggaatat cggaatgctg
    15961 attgggttac gtgccggctg gggtgtcggg aatgctttat ttgtctctac tgctttagca
    16021 acgatcgtca gtattttagt tggtaacaca gaaaaagcaa tcatgatgta tgaagcagca
    16081 cttggctgcg gaatggctgt tggccctcta gtcggtgggg tcttgggcag tggttcatgg
    16141 cgttatccat tttttggggt agcatttttg atgttttgcg gttttatagc cctagcggta
    16201 cttttacctg aaactgagaa accaaagaga aaagtaggcc tttgggaagg tgtcaaagcg
    16261 ttaagcaatc ataaacttcg ttcagttgga ctaatcgcat tgttatataa tttcggattc
    16321 tttactttac ttgcgtatgc gccattttta ttgattggct attctgaatt agaagtcggt
    16381 tttgtatttt ttggctgggg tgtgcttctg gctattgctt cgattttcgt tgcaccggct
    16441 cttgagcgga agtttacgac ttatcggaca attttagcag catttgtctt attcattatt
    16501 tgtttgctgt tattaggagc tggaacttct atgccagtcc ttgtaccgat cagcattgtg
    16561 ttagcaggat ttttccaagg aattatcaat acactgttga cgacgattgc tatggaaata
    16621 ccaggtctgc aacgaaacgt tgcttcgtcc agctatagtt ttgtccgctt tttcggtgga
    16681 gctttagcac catttattgc aggtgtgatt ggtgaacata ttggtgaaag ctatgcgttt
    16741 tattttgcag gtgccattgt tcttatcagt gtatgttgta tcatttatca ccgtaattat
    16801 tttgctacaa atgaagaaga ataagaaatc agaggttgtg aaaaagctgt cctgcatcaa
    16861 gtcataagaa
//
LOCUS       DACEOM010000227        15226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.076, whole genome shotgun sequence.
ACCESSION   DACEOM010000227 DACEOM010000000
VERSION     DACEOM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.076"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(134..577)
                     /locus_tag="IW781_003151"
     CDS             complement(134..577)
                     /locus_tag="IW781_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1184087.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IW781_003152"
     CDS             complement(795..2135)
                     /locus_tag="IW781_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1184088.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IW781_003153"
     CDS             2404..3300
                     /locus_tag="IW781_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1184089.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IW781_003154"
     CDS             complement(3450..4391)
                     /locus_tag="IW781_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1184090.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IW781_003155"
     CDS             4517..5638
                     /locus_tag="IW781_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1184091.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IW781_003156"
     CDS             5896..7398
                     /locus_tag="IW781_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1184092.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IW781_003157"
     CDS             7465..7992
                     /locus_tag="IW781_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1184093.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IW781_003158"
     CDS             8203..9264
                     /locus_tag="IW781_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1184094.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IW781_003159"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IW781_003159"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1184095.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IW781_003160"
     CDS             10177..12159
                     /locus_tag="IW781_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1184096.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IW781_003161"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IW781_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1184097.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IW781_003162"
     CDS             12881..14305
                     /locus_tag="IW781_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1184098.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..>15226)
                     /locus_tag="IW781_003163"
     CDS             complement(14486..>15226)
                     /locus_tag="IW781_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1184099.1"
                     /translation="LIKKKSTAVLSVLLVVLLIGTALLAKKYTTIIDPVEMTAQLFSA
                     TSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLVIYSVYLYLLAI
                     IVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSLVLMIACFINSS
                     GASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGNEEIMKQLTHLS
                     TNQMLFGNLAYIVLFLALGYLVFKRKNV"
BASE COUNT         4937 a         2753 c         3180 g         4356 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaa
//
LOCUS       DACEOM010000228        10677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.093, whole genome shotgun sequence.
ACCESSION   DACEOM010000228 DACEOM010000000
VERSION     DACEOM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.093"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            116..1000
                     /locus_tag="IW781_003164"
     CDS             116..1000
                     /locus_tag="IW781_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ1184100.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            1394..1891
                     /gene="dfrG"
                     /locus_tag="IW781_003165"
     CDS             1394..1891
                     /gene="dfrG"
                     /locus_tag="IW781_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1184101.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1963..3915)
                     /locus_tag="IW781_003166"
     CDS             complement(1963..3915)
                     /locus_tag="IW781_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1184102.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3922..4218)
                     /locus_tag="IW781_003167"
     CDS             complement(3922..4218)
                     /locus_tag="IW781_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1184103.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4784..5038
                     /locus_tag="IW781_003168"
     CDS             4784..5038
                     /locus_tag="IW781_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1184104.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            5435..7036
                     /locus_tag="IW781_003169"
     CDS             5435..7036
                     /locus_tag="IW781_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1184105.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            7270..7569
                     /locus_tag="IW781_003170"
     CDS             7270..7569
                     /locus_tag="IW781_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1184106.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            7704..8081
                     /locus_tag="IW781_003171"
     CDS             7704..8081
                     /locus_tag="IW781_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ1184107.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLIC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            8130..10493
                     /locus_tag="IW781_003172"
     CDS             8130..10493
                     /locus_tag="IW781_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1184108.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
BASE COUNT         3859 a         1466 c         1895 g         3457 t
ORIGIN      
        1 gattcagctt atattgcggt taaagacagt aaaagaatcc ctgaagcaac tttagaatat
       61 ttagagccaa ttaaattgtc tgttttttat tcactttatg agaaagaaat cgctgatgaa
      121 gcggtttaag gggagtaaaa tgagtaaaaa aataggcaaa gagtcattat cgctcggatt
      181 gaaaaaagaa attgttaaat ttaaaagtat gtatagagag ccggaattta gactcatttt
      241 tcaattagat atagtgagag ggctgaaaat tagttttagt tgtttaggtt atctggtctg
      301 gctattcatt gctagagtag gatggagcaa tgcttatagc ttttataaac tgttcgccgg
      361 aactgatcga ttaaaagaat cattagaggt gaaagaagcc ttaaatattt taggaagtct
      421 tgctttcagc ctgtttattt ttgctttatt tatcttacaa gtaggaaaaa taatgatgtc
      481 aacaaatgac aattatatta attatttgaa gccttttcaa aaaggaataa aaatcatttg
      541 tagacagggc acagttttat ttctacttag cctgtatgag catgaaattc attttcaaaa
      601 ttattataat acgacaacta aacttggtgc atttttactc ctatatttag ttagtaatat
      661 agctgaaaaa atgctgttta aacatattgt aaagcaagtc ttttctgata ttcaaaagga
      721 aagctattgt ttaaaaacac cttatggaaa tttaaaaaat gttgagctgt ttttatttaa
      781 aatggatagt aagtgggata aatatatcta tagaagtaaa gaagtatttt taactggtag
      841 ttataacaaa actcttcagg agagcctgta cgacacagga gcaatattta ataatgaacc
      901 tatggttttg aaagatttct ttggagatta tgaactagtt tggaatccaa aacagcttag
      961 tgaacagggg gcagaaaatg aatttgctga agttcactaa catagtgtac agaaaaacat
     1021 tatctgaaag aagaacgcct tcaaaagaaa gtacaagaac gattgggccg tacagaagac
     1081 taacgaaaga tcgtaaaaaa aaggaaagac aatgaaaatt aagtagttca gttttggagt
     1141 acaaaatgac aaaatgtact tcaaaatgga ctactttttt cattttaact tacataccaa
     1201 attacataca aaatgattta taaaatgcaa ttgaaaatga taattgaatg aaaatttgtg
     1261 attttgtggt attatgataa tataacttat agaaaaaatt gcttattcaa ctaacgggca
     1321 ggataatgag aaaaggtttt ttgatagaaa taagaaatta gatggtaaat tagttaaaaa
     1381 ggctgtgtta taaatgaaag tttctttgat tgctgcgatg gataagaata gagtgattgg
     1441 caaagagaat gacattcctt ggaggattcc caaggactgg gaatatgtta aaaatactac
     1501 aaagggacat ccgataatat taggtaggaa gaaccttgaa tcaatcggaa gagccttacc
     1561 tgacagaaga aatattattc tgacgagaga taaggggttt acctttaatg gttgtgaaat
     1621 tgttcattca atagaagatg tttttgagtt atgtaaaaac gaagaagaaa tttttatttt
     1681 cggaggagaa cagatttata atttgttttt cccttatgtt gagaaaatgt acatcacaaa
     1741 aatacatcat gaattcgaag gagatacttt ttttccagaa gtgaattatg aggaatggaa
     1801 tgaggtattt gcccaaaaag ggataaagaa tgataaaaat ccgtataact actattttca
     1861 tgtatatgaa agaaaaaact tattgagtta acgggtgcgt taattgaaga agaacagtaa
     1921 agataaagca caagagcttg ggattactcc aagctctttt tgtcatttga aataaattat
     1981 atctttccaa tcaaattgtt tatctgtaat ccctaatctt tgagcaggtg tcaaaacctt
     2041 tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa gttcttagaa ttgtaatcgc
     2101 aaattgagca tacttcggat tgaagttagc gtaaatatag ctcttcttat caccacgagc
     2161 tgttgttaat ggtctttcaa gagatgatat ataacgtctt atctgttgaa taaagctatt
     2221 ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt tcgtttggtt ccagagcaga
     2281 taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc ttagtggcta atggatgttt
     2341 gatagggtta tcggcatatt tgcgatatct ttctccatct ttactaatag cctcttcgtg
     2401 gaattgatga cttgttagta attcagttag ataatgagat gccaaagtcc gtagaaactt
     2461 tgttttgaat cctctgatat ctccccaatc gagcagttct gcttttgctt gtccgaattc
     2521 ttttaagcat tgttttctgc ttttcgtctt gttaacttgg caaataaaat gatgtgcatc
     2581 gctcaacctt aattctttcg tgaatactct ataaaaggcg tttctaatag agaagtcatc
     2641 atcactaatc attcgccatt cgtcggcatt aaccatttgt ttaattaacc aatagtgagc
     2701 agttgtagta taaggagcat caacatttaa accatcgaca aatctactcc tattagtaat
     2761 tttccctaat tcgtgaagat aagcattcct attttcgcta tcgttagcgg atggttcttg
     2821 tggataataa gaccaatcta acctatcatt tttacgacta aaagtattga gatggtcttc
     2881 tttgaatttt cttgtatctt catttaattc atccattgaa atattccaat cataggcaac
     2941 atcagaacgg aacacatatc ttgataagac ctcagcagat acaactatat acgtttgcat
     3001 attaaggtct tcaaaccccg tatatttctt tgaaccttgt cctttcttac ggacgttatt
     3061 taggtagtag tgcatcttgt ccgtattaag ccacatctcg ttaaatttct ttgtttgaag
     3121 gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc ctatacagcc actccagctt
     3181 atgatagtag gttccaactc caattcctaa aatatcacaa gtacgactga ctgaaaccct
     3241 tccaacaacc atcttagcga acattggaag aatggtgttc ttttgttggt gataagtagt
     3301 ggtttcttct ctttttggta gaacattagt gaacttctta cacgccttac attgatagcg
     3361 ttgagatttg cccctacttt tccccctttt gtaaaattgc ttcggctcat tgaatggggt
     3421 tgattcttct tccgagcagc cttctttatg aaattgataa tctggctcaa catcttggat
     3481 gctattgatt tctataaggc gtttaatttc ttcaacaaca gaccaattag ataaaggggt
     3541 tactgtacag ttttgcgata cacctctgcc aataggattg tcattgcagt acataccttt
     3601 atgaccttta tctttggaac taccagtcat tttatatcga cttggtttcc cttttacatc
     3661 tttgaatttg tgctgttctt tcccaaaatt acaacaaaac ggattgatgc agtaattata
     3721 ttgaatggga tattcagaac cgttccattt aaaagacgtt ggcaagaaca acattttgtt
     3781 ataccttgtt gcaaatcgtt ttggagttaa taaatcatat tgtttagccc tatcttttaa
     3841 ttcttcgttt gaaatcggaa ttttaacatc tacaatttca tcttccttcg tggctaatct
     3901 tttaagcttg gtcattgtct atcaacccac tctatctttg atacccattt tttcaacaat
     3961 ccgcttatta cttcgcttgt tggcaatcca ttcaataaac ttcttatcgt ctaaaatgtt
     4021 caataagaat tcatccactg cttcgctttc agtacgctct gcatattcag cgtattgctt
     4081 aactatcacc cttacttgct ctgatatttc ccaatccaca gacttagcgt tcttattttt
     4141 tggctcaaga aattgcatag gaaaatgcac ccctttttgc ttagtaaatt gtaaccattt
     4201 taacacacca atttgcattc ctacaataat tatttcacgt tcgcattttt atatacattt
     4261 taacttacat tttgtggtgt aaattaacaa caaacgttga tttaacaata aaaaacgagt
     4321 atcaaattca gttgcaattt gatactcatt taggtttaca ttttcacttt ttgtactcca
     4381 aaactgaact acttaaatga aaatgtcttt cctttttttt tgactacaag tagcctgaaa
     4441 atgaaatttc aaaagactac aagtagtctt ttgtggcaaa aataaagagc caacgaaagc
     4501 taccaacaat cgttgactcg taagactgtt cgtagtaaga aaaatctttg gcaatccacg
     4561 aagtctttat ttcataattt atataaatga ttatacagtc actttcctga atttgcaagt
     4621 aaaaaagcta agaaatattg tttaagcgtt tccttaaaag aatctttaaa taaatttttt
     4681 tccaattgaa aacatgacta caagtagact atttctttta ttccaagttg actacaagta
     4741 gtcaaggtga tataatatat atatcaagaa aggagaaaca aaaatgtata acgtaggaga
     4801 tcaaatagat gagatcgtaa aaataggtaa tgttaaactt gttaaaagta agtactatat
     4861 ttcagaaact gaaataattt ttaatactga atcaggatgt attctaattt ggaaactgcc
     4921 ttttcccaaa gacgaaacaa cactgaaaaa aataaatact ttaaaaatca gatattgcat
     4981 tcatgaaatt attgataaaa atacttatgt tataaatcaa gtaaaagtga tggggtaaaa
     5041 atcttttaaa aaaactcttg ttaaaagtaa ttctattagc ttataatgtt ggtattagtt
     5101 ctgaagccaa aggaacacta aaaaattcta gtagaaaagt tagttacaga ctctctacta
     5161 tgtatgaatg taacgaaaaa aatagcgaaa aagataatta aataagacta ccaatcttat
     5221 ttaatggaac ttctgtagta agaaaagcga tttggcaatc cacgaagtcc accaagatga
     5281 gctaccaact cattttaatt tttgcacgtt taaagtttag catttaagtg cttactctaa
     5341 agacatagtg taattttagg actccttatt acggaatcta aagttaccct atgtcttttt
     5401 tgctattcaa aattgatagc aaggagaaag tgcaatgttg aatagttgcg agtatgcgga
     5461 cgttttagct aaaattctta aaaatggaat taaacagttt aatagcaaaa atctagttgc
     5521 ttttaaaaag aatagcaata agattggtgc tattgccgga tttacttcta aaacaaacat
     5581 gataaagact aaaggtctta tatatcgttc tattgatcgt gtcttggaag attcagatgt
     5641 attaacacac tggactccta acgtgtattc atggcttggt ggcggtgaaa acaaaacaat
     5701 ttttggtcac aaagaagcta acttaattca gattaatact tttgtagcag atattgacta
     5761 tccagtagga gaaaagaaaa cggatttgaa tttactagtt ctttatctat tggaagaagg
     5821 gttattgcct acactgattg tagatacgcc aaaaggttat cattgctatt ttctgattca
     5881 aaactataac caagaaactg attcatttga taaggcaagc tatatttcta attcaaatga
     5941 ttttaaatct ttgagagttg ctaaaagaat atcagaaaat atcagaaaat ctatccaaaa
     6001 tcgtttacca caagtcgata tgggctgtaa tcattttggt atttttagat ttccaactaa
     6061 aaaaaatatt gtacattatg aacctaattt cgtagatacg tttgaagggt atttgaaatg
     6121 gtcaaaagac tttgaagaaa aagaatatgc tttaaagagg tctaacttgt cattattagc
     6181 tcctaattct actggcaaga aagggtctaa acgacaaatt gatactaaat ggtacgactt
     6241 tttaattcat acggatattg ctgaaggaga tagaaataaa actattttta ccttagccct
     6301 ggcatgtaaa tcaagtggct tatctattgg cgagtgtatg gatcaattgg atcaacttca
     6361 attcaataat gatttgtcct attctgaagt tgagagaaca ataagaagtg cttatcgtgg
     6421 cgattacaaa ggtgctaaaa gttcatatgt caatgaaatt atcgaaaatt atgctacacc
     6481 taccgagttt gaaaaatact caataaatgt aaatagacaa gctgttaaac gtaaagggaa
     6541 tcgtgtcgat ccgtctaatt gggtgaaatt tgctaaacct agagaagaac gaaaatatag
     6601 tcattttgaa gaaagtaaag ctgatttatt agcttactta gaaagaaaac aaagatcatt
     6661 gaaaaaaaat gagttgtttt taagtgtctc tatgggaact atatctgaag aaacacaaat
     6721 tcctgtatct acgttaaagg tgattttaaa agagcttaga acggcgaata cagtgatatt
     6781 acgaacgtct cgtggtcggt atggatcgac taaaatagca acaagcaaat ttgtggaagc
     6841 taaaatatta aatacagtta ttcagaataa aaaagataaa caagtatttg tcttagaatt
     6901 attaggaaca gcaaatagcc acgtagaaac gattttaaga cgttatgaag acaaaacagt
     6961 accgttccat gcgaacatac tgaaagaaag agaaaagagc aaacatgcgc caattagagg
     7021 agctaataca ggttagagtt ctatttattt ttttgatttt taaatagcta aattgccaat
     7081 taacatatct agtgtttctg cccactccta ttcctgtttt agcgtatact aatgattagt
     7141 agttagtact ctcttttatg tatggagtgt atagaactat ttttacttgt gtcttttccc
     7201 ttaatccacg tgtacggaat acagcttata actgcgtatg cagttatgtt tgaaggagga
     7261 agattttctt tggtaattaa gaaatatgtt tactcacacc cgatcaatgt atttattatc
     7321 ggtaagctag gtttatctgt tgaggtgttt tgtgagttat atggatttaa acaaggaact
     7381 ctatcgtctt ggattactag agagcgtgag gtagctgatt tgcctatttc ttttttgtat
     7441 gctttgtctc tagcttcagg agggaatatg aatgaggttt atgattgctt gtgtgagtat
     7501 gaaaaagagt ataaagagta tttgaaaatt tcagccccaa aacggaataa aaaatatatt
     7561 gaagattaga gaaagaagaa aagaataatt tgtttttttg actacaagta gacagatagc
     7621 tactctgttt tttgatagtt tgatattggc tgtttttttg ttagaattaa tatatatcag
     7681 gtaaataaaa aagggggcta ttaatgaata ttgatgggat ttataatgaa tcagatttta
     7741 aaaaaggcat agtagatatt gatagaaaag agtatttatt tcctgatgaa ataggtttat
     7801 ttaaaattgt aggtgaaact gtctgtaggt attgggggaa gaaaggaaat ataattttac
     7861 ttgttgatac gattgaggga agaaagttaa aattttcgtt ttggagtaat acaaaatatt
     7921 gttgtgaacc tgtacctaaa agggaagtag atgtggctaa agaaagtgta cgatttttac
     7981 ctgatcgatc aaaaataact ttaatttgtg aacggacaag taatgctaaa tttacaagaa
     8041 ttagagatat actactttcg actacaagta gtcagtcata gagtaagaac ttttggttaa
     8101 aaatcaattg aagaaagtgg aggaagtata tggcagtagt tatattagca gaaaaaccag
     8161 atcaaggagc aaaatttgct tcagcattgg ccggaaaaaa attatcgaaa aagagtggga
     8221 agtatgaatt taaaagtgaa atatttggag atactattgt tacttgggga ataggtcatt
     8281 tggtaggatt atcattacct gaaaagtatg agtggctacc gaataaagat aaatggcatt
     8341 tagataattt gccttttcta cctaaattca atgaattaaa gtatgaagtc tctaaaggaa
     8401 aaaaccaaca attttcaata gtgaagtcat gtttagaaaa tgcagatgag ataataattg
     8461 ctactgatcc ggatagagaa ggagaaaata ttgcttataa tattttcaaa ttatgcaagc
     8521 agtcgatttt taataaaccg ataaaaagat tatggattaa ttctttggtt caaaaagaaa
     8581 ttatcaaagg atttaaaaat ctgcgaaatt ctgaagaaac gatctcttac ttcaaagaag
     8641 ctaatgctag acaaattgct gactatctag tagggatgaa ttatacagag ttgtattcat
     8701 taaagttgca agaaaaaggt atatcgggtg tggtatcact cggtcgagtt cagacaccgg
     8761 taaacacact tgtggttgaa aacgatttgg caattagaaa ttttagaccg gaaaaatata
     8821 aaacgattga atgtcataca accgataagc aaccaaaagc aatatttaag aatagtcttg
     8881 aatactttaa attagaaaaa tttgaagaag acactaaaaa attcggcctt gattccgcca
     8941 gtactggaat cataaaaaaa attgaggtta gtaaaaagca aacagaacca caaaaattgt
     9001 ttagcttggg tggtattcaa gaatacgcta ataaaaaatg gaaatattct gctaaaaaaa
     9061 cagataaaat tatccaaagt ctatatgatc gtggatattt atcttatcca agaaccgata
     9121 gtgaattgat aactacaaat gaatttgagt atttaaaagc gaatttagac gtgtataaga
     9181 gtgttttagg gatcagtatt gatacacctt gtttagagcc taaaaagcgg tttgtagata
     9241 atgacaaagt attagagcat tatgcactaa taccaacaat ggatattcct gatatagcaa
     9301 atctgacatt agatgaaaaa aatatttatg agatcgttac taggcgtgcg tgtctaatgt
     9361 ttgcagaacc ttacaagtac gaaaatacaa aagttacatt agatgtaaac ggcttagaat
     9421 ttaccacaag tggtaatgta cctatatctt taggttggaa gcaagtagat actaatttag
     9481 atgaatctga tgataaagag gaaacattac ctaaatttca tgaacaagag acaataaata
     9541 tagatattag atttattgat aaattaacta aggaacctaa aaggttgact gaaggtaaat
     9601 tattaggtaa aaccggtatt atggcgaaat tagggctagg aacgcccgct acacgttcta
     9661 atattgtaga cactcttcta aatagggaat atattaagat agaaaacacg aatgtttatc
     9721 ctactccaaa gggatattta ttatgggatt tgacaaagaa taaagaccta ttgattggaa
     9781 aaccagaaaa tacagcgata tgggaagaat cacttgggaa aattgcgcaa ggtagctata
     9841 cacaggaagc ttttttaaga aatatctata actatatcaa taagactgtt tcagatttga
     9901 aaaaagtaga atttacttcg gattatttag aaaagactgt aaatcaaaat aaatttgata
     9961 taggtagtta tcaagtggaa gaaaagaaaa aagtttattc tattttaaat attactagtg
    10021 gtgaacagtt cgtgatattt aaaaatatat caggaaaagc aatcaataaa aaagttcttc
    10081 aagaactatt actaaataat cgtacagata aagtcctttc cggctttaag tcaaaagaag
    10141 gaaaagcttt taatgcaaga ttacttttcg atccaaatgt gatgaaagtg gtaatggagt
    10201 ttgaaaagaa cagtccaaaa ggcgataaaa agagcaaaca cgatacagta aatggttatg
    10261 aaatagttga aaaagcaaaa gtatttgaaa taaaagaatt agcaacaggt gaaagtttta
    10321 ttttctataa aaataatagt gggaaaactt taagttcaaa aacagtcaaa gaattgttag
    10381 aaaatgggaa aactaaagag aaagtaacgg gttttataag taaagacaat aaaaaatata
    10441 gtgcttatct tgtctttgat aataaaaaca aagttataaa gaaagaattt taattcttag
    10501 tggcggaaat catttgtttt tccgcttatt tttattcaaa agtgcccaaa atataggtat
    10561 actgaattaa ggataaatac aaaactagtt agaggacagt tgaaaaaata caaacaaatt
    10621 agattgctat tttgttaaat tggaagtaga ataataccgt tttgatatgt atagtgt
//
LOCUS       DACEOM010000229         8061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.112, whole genome shotgun sequence.
ACCESSION   DACEOM010000229 DACEOM010000000
VERSION     DACEOM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.112"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1564
                     /locus_tag="IW781_003173"
     CDS             128..1564
                     /locus_tag="IW781_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1184109.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IW781_003174"
     CDS             1753..4200
                     /locus_tag="IW781_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1184110.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IW781_003175"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IW781_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1184111.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IW781_003176"
     CDS             complement(5690..6793)
                     /locus_tag="IW781_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1184112.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW781_003177"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW781_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1184113.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DACEOM010000230         7371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.119, whole genome shotgun sequence.
ACCESSION   DACEOM010000230 DACEOM010000000
VERSION     DACEOM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.119"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW781_003178"
     CDS             245..1213
                     /locus_tag="IW781_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1184114.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW781_003179"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW781_003179"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1184115.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW781_003180"
     CDS             2243..2851
                     /locus_tag="IW781_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1184116.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /gene="vanY"
                     /locus_tag="IW781_003181"
     CDS             3279..4190
                     /gene="vanY"
                     /locus_tag="IW781_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1184117.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ"
                     /locus_tag="IW781_003182"
     CDS             4343..4828
                     /gene="vanZ"
                     /locus_tag="IW781_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1184118.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5115..5444)
                     /locus_tag="IW781_003183"
     CDS             complement(5115..5444)
                     /locus_tag="IW781_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1184119.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5749..6078
                     /locus_tag="IW781_003184"
     CDS             5749..6078
                     /locus_tag="IW781_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1184120.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6068..6355
                     /locus_tag="IW781_003185"
     CDS             6068..6355
                     /locus_tag="IW781_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1184121.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(6634..7197)
                     /locus_tag="IW781_003186"
     CDS             complement(6634..7197)
                     /locus_tag="IW781_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1184122.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2394 a         1249 c         1490 g         2238 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacta tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc
     5041 cttaacgttg taaatccgca ttttcctgac ggtaccccca aactaagatt tcatagatag
     5101 attttctgat aactttaaaa aacacgaata gcggaaagga tgtcttcatt cgctaaagag
     5161 taataaacta cttttccatc ttgacgtgat tttgccattc catgtttttt taaaaaacgt
     5221 aaatggtgtg acgttgtagc gacactagca gtaagtattt ctgctaaatc acagacgcac
     5281 atttccttat aggtttctaa agcatagaaa attttaattc tagaaaaatc gctgaaacat
     5341 ttccctaaca caagtaagtt tgaaaaatct ttttgatcca atttattttt tattccattt
     5401 actttttctt tatgtacgac agtcacttgg caaacttttt tcattcatct cacctcacat
     5461 tcaaacgatt atttgaatga agtataccta tttgatcact cttttgtcaa tctaccttaa
     5521 ttccaataca ctattataag caatttcatg tattgaagta aacttacttg caagtgcgtt
     5581 tttgattcca agttaaaaac atattattat tctacataat ttcctaatca aataaaactc
     5641 ctttgaaaat tgttctaaag gagttttatt attttgtatt tctactaaaa atagcgtaca
     5701 atgtaattac taagtaatta cgaaatgtaa aataatggag gaaataatat gattacaaaa
     5761 acaagaaaac aaggaaattc tattatgtta acggtcccta aagaattcga tgtgcccaat
     5821 ggtgttgaag tagaagctaa gcttgtagag aatggtatcc tttatgagtt tgtagagcct
     5881 aaaaaagaat tttttgattt tagtgaagat attttggctg atattctttc agaaggttat
     5941 agcaaacaag agatcttaaa agaatttaga aatagaaaaa atgcgttaac ttctgcattt
     6001 aaatctattg ctgaagatac tgttgcaaat tctaaaccga tgacaaaaga ggagttggca
     6061 gcagaaattg gcttatgaga ttctaccttc taaacacgta ataaagtatc taaaaaaatt
     6121 aaaagagaaa acattaaaag aacaattttt aacgataatt tatgatgaga tagcagttcg
     6181 tcctcattcg ggagaacaaa aaacgggtga tttatcagga atttgggcta ttggattcaa
     6241 gtatgcaggt acgacatatc gcgttgcata tgaaattaaa gataatgcag tgatacctat
     6301 cttactatgt ggaacgcatg aaaattttta tgaacaatta aaaaagatta ggtaagaacc
     6361 caatgtttca acttgagagg atagacgaca ctttaaaaga taatttttaa cagctgagag
     6421 ttgtaatacc ctataatttt aaaccactaa cctatatatt tatgaaaaat agaaaaaatt
     6481 aaaacgcatt ttaagcctct ttcaaattaa aagcttcatc cttatgcaca aagagggctt
     6541 aaaatgcgtt attttatatt gttttttagt aataataaaa aaatcactct aattctccca
     6601 taaatattat agattcctaa agattaaaat agattattcc ctattgtctt ttaagtagcg
     6661 gtagagtgtt gccttgctaa taccagtcat ttccgtaatt tcttttacgc tataaacctg
     6721 acttttatac aattttactg ctcgatcaac atctttctgg ttcactcgtg gacggccacc
     6781 tttacgacct ctcgcacgtg cacttttcaa accttctttg gtacgttcaa caattaaatc
     6841 acgttcgaac tgactaaatg cttggaagac agtcatcatt aagcgtccgt gaggcgtgct
     6901 ggtatcgaag ttttctttca agctcaccag cttaacatgg tgttcttcaa aataggtaac
     6961 aaggtcaatc aaatcctttg tactacgacc caatcgagaa aaactttcta caattaccgt
     7021 atctcccgga cgaattttat cttttaaacg atttagttgg ggacgaaccg ctttggttcc
     7081 cgtcattttc tcagttagga tttcattaca attttgttca ttgagtaagt ctaattgacg
     7141 atttagttct tgtgaacgtg tactcactcg ggcataacca taaatgtatc cactcatgct
     7201 tttcttcctt tcgtacttta attctggctt gattgtatca taaaagggag ttatagatac
     7261 atagttaatg caacaagtta tgatacaaaa tatgacctct tttatttaat aaaagaggtc
     7321 atttcatttt gtctcataat cgattgtttt tgatacgagt gtgtatacga a
//
LOCUS       DACEOM010000231         6088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.126, whole genome shotgun sequence.
ACCESSION   DACEOM010000231 DACEOM010000000
VERSION     DACEOM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.126"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..219
                     /locus_tag="IW781_003187"
     CDS             <1..219
                     /locus_tag="IW781_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAQ1184123.1"
                     /translation="KKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGKETLVKG
                     IKNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            382..1047
                     /locus_tag="IW781_003188"
                     /pseudo
     CDS             382..1047
                     /locus_tag="IW781_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1083..1328
                     /locus_tag="IW781_003189"
     CDS             1083..1328
                     /locus_tag="IW781_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1184124.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1431..2300
                     /locus_tag="IW781_003190"
     CDS             1431..2300
                     /locus_tag="IW781_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1184125.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2281..3015
                     /locus_tag="IW781_003191"
     CDS             2281..3015
                     /locus_tag="IW781_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1184126.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3048..3956
                     /locus_tag="IW781_003192"
     CDS             3048..3956
                     /locus_tag="IW781_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1184127.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3953..4495
                     /gene="sat4"
                     /locus_tag="IW781_003193"
     CDS             3953..4495
                     /gene="sat4"
                     /locus_tag="IW781_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1184128.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4588..5382
                     /locus_tag="IW781_003194"
                     /note="aph(3')-IIIa"
     CDS             4588..5382
                     /locus_tag="IW781_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1184129.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5490..5678)
                     /locus_tag="IW781_003195"
                     /pseudo
     CDS             complement(5490..5678)
                     /locus_tag="IW781_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5658..>6088
                     /locus_tag="IW781_003196"
     CDS             5658..>6088
                     /locus_tag="IW781_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1184130.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAA"
BASE COUNT         2104 a          902 c         1330 g         1752 t
ORIGIN      
        1 aaaaaatcgt tttatggttg ttcaaattat cctgaatgta agtttacttt agctgaacat
       61 tttagaaaga aaaaactaac caaaacgaat gtaaaagaat tactggaggg aaaagaaacc
      121 ctggtaaaag gaatcaaaaa caaagagaaa aagccctaca atgccgttgt aaaaattggg
      181 gaaaagggat atattgattt tatctctttt tcaaaataaa catggggctg acccaaaagt
      241 agctcctata tgaaaaatag aggtctttcc atttagatag tagtggaaag acctctattt
      301 atcttttgaa agtgaataca aaaataaagc acactctgct ataatatagg tgattaatcc
      361 atttgagaga ggtgctttat catgtatcaa aattatacca taaaccaact ttgtttgcct
      421 atagatcttg aaattaaatt agaagaaaat gattttgctc atgccattgt gcagtttgtt
      481 gactctattc ccgatgaagt ctttcttcct tattatcaat ctatgggtag accccaatat
      541 catcctcgta tgctactttc cattattctt tgtgcctata ttcaaggggt ttattcgggc
      601 cgccaaatac aaaatatgct gatagatagt attcggatgc gctacttatc tcaagagcaa
      661 ttccctaact ttcgtactat taatcgtttt cgcgttcatc cgattatgaa cgatattcta
      721 gatcattcat ttgttcaatt tcgtgagcta ctggttcaaa gtggcttaat cagtggcagt
      781 gctctataca ttgatggtac aaaaattgaa gcggatgcga ataaatactc tttcgtttgg
      841 aaaaaaagta ttttaaagta taaggatagc cttgatcaaa aagcccttga aaactaccaa
      901 caaatggtgg aaacaaaaat cttacctgag ttaatagatg atttaaagga tgaactttct
      961 atagaagaga ttgaaaggat tcgtcaatct ttggaagaaa aggaaaatca attaattgaa
     1021 gcgattgacc aaaccgaaac agtagaaggg ggctgaccca ttattaataa ggagtggaca
     1081 acatgattga tgtaaatgag ctattggaag aagctattag agaaactgaa aatctaaatg
     1141 atggcgaagt ttttcttgtt aaagatttat tcaagggata tgtggggaat aggataccca
     1201 gaaaggatcg acttctgctt ggaacgttat ttttaaacca tgttagcaaa atgaatggta
     1261 atttaaaggc tattgaaaag acttcatcta atcagcagag gtataaaaag acaattgata
     1321 agttttaact atcttttgtg aaattaaaaa aattataaat atgttgagta gaagcaaaag
     1381 atttggcttg gaatttttac agcagtgaat cgataaaagg agcatgcttg atggtagata
     1441 atattattaa atcagtagca gagaaattat cctctctgtc ttatatagaa ggtattgttt
     1501 taggtggttc acgtgcaagg ggcacccata cagaggattc ggatatagat atcggcatct
     1561 attacaattc agaatcattt gacataaata ctattaatca attcgctaca aagctggatg
     1621 atgagcatag aaataacctt gttgtacctc ccggagcatg gggtgattgg attaatggcg
     1681 gcggatggtt agtcataaac gggtatcatg tggatttaat tttacgtgat attaaacgtg
     1741 tggaacaaat aatgaaagat acagagcacg gaattgttac tgccaattat cagactgggc
     1801 atccccatgg ttatattagt gcaatgtatc gaggagaatt agcgattagc aaaatactat
     1861 atgctaagaa tgaaagctta tgcgaattaa aaaaacaggc agaaacttat cccaatgctt
     1921 tgcagaaaag tttagttaac ttttttatgt ttgaagcagg gttctcttta atgtttgtaa
     1981 aagcaaattc gggaacagac gataaatatt atattgcggg tcatgttttt cgtatagttt
     2041 catgtttaaa tcaagtgtta tttgcatgta ataatgctta ttgtatcaac gaaaagaaag
     2101 ctataaaact gcttgaaact tttgaacata aacctgaaaa atataccgag aaggtaaatc
     2161 atatttttga agtactcggt atctcacttt ttgaatgcta cgacatgacc gagaagcttt
     2221 ataatgaagt gaatgaaatt gtatcggaga taaataactt tttaaacgag gagagttcag
     2281 atgaaagaaa acaaatatga tgataatata ttttttcaaa aatacagtca aatgagtcgc
     2341 tcgcagaaag gactggctgg tgcgggagaa tgggagactt tgaaaaagat gctacctgat
     2401 tttaagggta agcgtgtgct tgatttagga tgcggctatg gatggcactg tatatatgcg
     2461 atggaaaacg gtgcttcctc tgtagtaggt gttgatattt ctcataaaat gctcgaagta
     2521 gcaaaaggaa aaacccattt tccacagatt gaatatgaat gctgtgccat agaagatgtg
     2581 gatttcccag aggagagctt tgatgtaata ctaagttcgc ttgcgtttca ttatgtagca
     2641 gactatgaga atttaataaa aaagatatat aggatgctga aggctggtgg caatttagtt
     2701 tttacagttg aacatcctgt ttttactgct catggaacac aagactggta ttataacgaa
     2761 aaaggagaaa tactgcattt cccggtggac aattattatt atgagggcaa acggacagct
     2821 atgtttttgg aagaaaaggt tacaaaatat catagaacac tgaccacata tctaaataca
     2881 ctgctttcaa atagttttat aataaatcag attgtggagc cacagccgcc agagaacatg
     2941 atggatattc cggggatggc ggatgaaatg cgacgcccaa tgatgctgat tgtatcggca
     3001 aaaaagaaga tgtaataata tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa
     3061 aagaaatgat ggatttagta ctttctttag cagaacagga tgaacgtatt cgaattgtga
     3121 cccttgaggg gtcacgcgca aatattaata tacctaaaga tgaatttcag gattatgata
     3181 ttacatattt tgtaagtgat atagaaccgt ttatatctaa tgatgactgg cttaatcaat
     3241 ttgggaatat aataatgatg caaaagccgg aggatatgga attattccca cctgaagaaa
     3301 agggattttc ctatcttatg ctatttgatg attacaataa aattgatctt accttattgc
     3361 ccttggaaga gttagataat tacctaaagg gcgataaatt aataaaggtt ctaattgata
     3421 aagattgtag aattaaaagg gacatagttc cgactgatat agattatcat gtaagaaagc
     3481 caagcgcaag ggagtatgat gattgctgca atgaattttg gaatgtaaca ccttatgtta
     3541 ttaaaggatt gtgccgtaag gaaattttat ttgctattga tcattttaat cagattgttc
     3601 gccatgagct gctgagaatg atatcatgga aggtcggcat cgaaacaggc tttaaattaa
     3661 gtgtaggcaa gaactataag tttattgaaa ggtatatatc cgaggatttg tgggagaaac
     3721 ttttgtccac ctaccggatg gattcctatg aaaacatatg ggaagcatta tttctatgcc
     3781 atcaattgtt cagggcggta tccggtgagg tggcggaaag gcttcattat gcctatccgg
     3841 agtatgatag gaatataaca aaatatacca gggacatgta taaaaaatac actggtaaaa
     3901 ccggctgcct ggatagcaca tatgccgctg atatagaaga gaggcgggaa cagtgattac
     3961 agaaatgaaa gcagggcacc tgaaagatat cgataaaccc agcgaaccat ttgaggtgat
     4021 aggtaagatt ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga
     4081 agcgccatat ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg
     4141 ccttgaatat attgacaata ctgataagat aatatatctt tactaccaag acgataaatg
     4201 cgtcggaaaa gttaaactgc gaaaaaattg gaaccggtac gcttatatag aagatatcgc
     4261 cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga
     4321 atgggcaaag cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat
     4381 agcttgtaaa ttctatcata attgtggttt caaaatcggc tccgtcgata ctatgttata
     4441 cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
     4501 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
     4561 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
     4621 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
     4681 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
     4741 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
     4801 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
     4861 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
     4921 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
     4981 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
     5041 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
     5101 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
     5161 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
     5221 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat
     5281 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     5341 gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga tttagatgtc
     5401 taaaaagctt taactacaag ctttttagac atctaatctt ttctgaagta catccgcaac
     5461 tgtccatact ctgatgtttt atatcttttc taaaagttcg ctagatagag ttctatatta
     5521 gagatgtaaa gagttttggt gaagaggttt gtgagtatgg cttttataac cagggtgcaa
     5581 tgagaagcac taacagcagc tagcttaaga acgcatagga tatacttttt atggaagtca
     5641 cagaggagga ataaaatttg aacaggatta acagagtaac ctctattctt attcagctgc
     5701 aatcaaaaaa aataattcct gccaaagaaa ttgcacagcg atttaatata agcttaagga
     5761 cagtttacag agatatccgg acacttgaag aagccggaat accaattgga tctgaggccg
     5821 gaaaaggata ttttcttgta gagggcttcc tacttccgcc ggtaatgttt acagcggcgg
     5881 aagtgggtgc attgattaca gcagggaaat ttttaaattg ccatggagat gaatcattta
     5941 taaaggattt tgattcagct atgtataaaa tcaaatctat tttgaagcat ggtgaaaaaa
     6001 actatgcaca ggagttggag aacagtatta acgtgtacag cacatctgga cagaaaaata
     6061 cattggcgga taacgttatt gcagcaat
//
LOCUS       DACEOM010000232         4997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.137, whole genome shotgun sequence.
ACCESSION   DACEOM010000232 DACEOM010000000
VERSION     DACEOM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.137"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..2180)
                     /locus_tag="IW781_003197"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IW781_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IW781_003198"
     CDS             2326..2901
                     /locus_tag="IW781_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1184131.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IW781_003199"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IW781_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1184132.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IW781_003200"
     CDS             3788..4942
                     /locus_tag="IW781_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1184133.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1561 a          939 c          967 g         1530 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatctta
//
LOCUS       DACEOM010000233         1012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.202, whole genome shotgun sequence.
ACCESSION   DACEOM010000233 DACEOM010000000
VERSION     DACEOM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.202"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..133
                     /locus_tag="IW781_003201"
     CDS             50..133
                     /locus_tag="IW781_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1184134.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            258..995
                     /gene="erm(B)"
                     /locus_tag="IW781_003202"
     CDS             258..995
                     /gene="erm(B)"
                     /locus_tag="IW781_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1184135.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          382 a          167 c          156 g          307 t
ORIGIN      
        1 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
       61 ccaaatgcgt aatgtagata aaacatctac tgttttgaaa cagactaaaa acagtgatta
      121 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      181 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttac agatgtaata
      241 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      301 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      361 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      421 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      481 ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      541 ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      601 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      661 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      721 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      781 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      841 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      901 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
      961 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt tt
//
LOCUS       DACEOM010000234         1012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.203, whole genome shotgun sequence.
ACCESSION   DACEOM010000234 DACEOM010000000
VERSION     DACEOM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.203"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..133
                     /locus_tag="IW781_003203"
     CDS             50..133
                     /locus_tag="IW781_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1184136.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            258..995
                     /gene="erm(B)"
                     /locus_tag="IW781_003204"
     CDS             258..995
                     /gene="erm(B)"
                     /locus_tag="IW781_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1184137.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          382 a          167 c          156 g          307 t
ORIGIN      
        1 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
       61 ccaaatgcgt aatgtagata aaacatctac tgttttgaaa cagactaaaa acagtgatta
      121 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      181 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttac agatgtaata
      241 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      301 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      361 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      421 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaata
      481 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      541 ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      601 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      661 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      721 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      781 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      841 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      901 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
      961 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt tt
//
LOCUS       DACEOM010000235          550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.229, whole genome shotgun sequence.
ACCESSION   DACEOM010000235 DACEOM010000000
VERSION     DACEOM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.229"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..474
                     /locus_tag="IW781_003205"
     CDS             <1..474
                     /locus_tag="IW781_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HAQ1184138.1"
                     /translation="DNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEG
                     KKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGED
                     ILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          240 a           32 c           98 g          180 t
ORIGIN      
        1 gataataaac aaaatgtatt agaagagtat atattgttgc gtgaaactat ttataatgat
       61 ttaactgata tagaaaaaga ttatatagaa agttttatgg aaagactaaa tgcaacaaca
      121 gtttttgagg gtaaaaagtg tttatgccat aatgatttta gttgtaatca tctattgtta
      181 gatggcaata atagattaac tggaataatt gattttggag attctggaat tatagatgaa
      241 tattgtgatt ttatatactt acttgaagat agtgaagaag aaataggaac aaattttgga
      301 gaagatatat taagaatgta tggaaatata gatattgaga aagcaaaaga atatcaagat
      361 atagttgaag aatattatcc tattgaaact attgtttatg gaattaaaaa tattaaacag
      421 gaatttatcg aaaatggtag aaaagaaatt tataaaagga cttataaaga ttgattatat
      481 aatatatgaa aagctattat aaaagacatt gataaagtcc gtataattgt gtaaaagtaa
      541 aaaggccata
//
LOCUS       DACEOM010000236          397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E493
            SAMN07141545-rid9868443.guided.240, whole genome shotgun sequence.
ACCESSION   DACEOM010000236 DACEOM010000000
VERSION     DACEOM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141545
            Sequence Read Archive: SRR5811390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740720.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740720.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:02:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,206
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,112
            CDSs (with protein)               :: 3,112
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141545-rid9868443.guided.240"
                     /isolate="E493"
                     /isolation_source="PERIANAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Dec-1994"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>397
                     /locus_tag="IW781_003206"
     CDS             <1..>397
                     /locus_tag="IW781_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1184139.1"
                     /translation="LYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLK
                     KERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNA
                     TNVKAMKYLIEHYFDNFKVDSIEIIGSGYD"
BASE COUNT          160 a           37 c           74 g          126 t
ORIGIN      
        1 gttatatact gattatcatt atccaaaaac tgatgagata gtctatggta tggatcaatt
       61 tataggagag ccaaattatt ggagtaaagg aattggtaca agatatatta aattgatttt
      121 tgaatttttg aaaaaagaaa gaaatgctaa tgcagttatt ttagaccctc ataaaaataa
      181 tccaagagca ataagggcat accaaaaatc tggttttaga attattgaag atttgccaga
      241 acatgaatta cacgagggca aaaaagaaga ttgttattta atggaatata gatatgatga
      301 taatgccaca aatgttaagg caatgaaata tttaattgag cattactttg ataatttcaa
      361 agtagatagt attgaaataa tcggtagtgg ttatgat
//
